Microscopy ListServer Archives  


File Requested = MicroscopyArchive09.txt
Retrival Software Version=NJZ07060908

From: zaluzec-at-microscopy.com
Date: Thu, 1 Jan 2009 00:00:00 -0600
Subject: [Microscopy] Administrivia: Listserver Archives for 2008

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to yet another year of operation of the Microscopy Listserver.
It was another productive year for all of you, during 2008, the listserver
delivered 2238 messages (} 230 Gb of Email) to ~ 3000 subscribers
around the world, with only minimal hassels (that I know about).

The complete Microscopy Listserver Archives for 2008-1993 are on-line
at http://www.microscopy.com. Those of you that use the archive facility will
notice I have decided to no longer produce monthly snap shots of the posting
for the search engines. Instead you will only be able to search a full year
at a time. I no longer believe these smaller monthly snapshots are important
with the ubiquitous access to high speed network connections.


Cheers,

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jan 1 08:37:35 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n01EbYjw021867
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2009 08:37:34 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c582825929fb-at-[206.69.208.22]}
6, 11 -- Date: Thu, 1 Jan 2009 08:37:32 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
6, 11 -- Subject: Administrivia: Listserver Archives for 2008
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================





From: rosemary.white-at-csiro.au
Date: Thu, 1 Jan 2009 02:28:38 -0600
Subject: [Microscopy] Re: viaWWW: Stains for proteins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mark,

One stain is tannic acid/ferric chloride, it's a good alternative to osmium.
I've usually used this for membranes, but it does stain things like
cytoskeletal proteins and ribosomes pretty well too - it probably binds to
certain charged surfaces or groups, like most fixatives.

You can do this a number of ways, the two commonest ones are to either fix
first in aldehyde (glutaraldehyde or formaldehyde) in appropriate buffer,
rinse well, then stain in 1% tannic acid, rinse well, then stain in 1%
ferric chloride. The tissue will go black, just as if it has been
osmicated. Then continue as usual - dehydrate, embed.

The alternative is to add the tannic acid in with the aldehyde fixative,
which is the way I've usually used it for plant tissues.

The ferric chloride is just made up in distilled water, and you can vary the
concentration of either TA or FeCl3, and vary the time, etc.

I imagine you are fixing animal/human tissue (I work on plants only), so
just go with whatever is the standard TEM protocol and add the TA/FeCl3 as
above. It used to be the case that for reliable results you had to use
Mallinkrodt tannic acid, but the manufacturing process used by other
companies may be better now.

There are a couple of older references to this which I can dig out - the
paper we cite them in is pre-web.....

cheers,
from 2009 already downunder...
Rosemary White

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334





On 1/01/09 7:46 AM, "twigg-at-estd.nrl.navy.mil" {twigg-at-estd.nrl.navy.mil}
wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both twigg-at-estd.nrl.navy.mil as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: twigg-at-estd.nrl.navy.mil
} Name: Mark Twigg
}
} Organization: Naval Research Laboratory
}
} Title-Subject: [Filtered] Stains for proteins
}
} Question: I have a new project which may entail staining proteins so
} that there is more contrast for TEM imaging. One collaborator
} recommended osmium tetroxide, but I read on the web that it is rather
} poisonous and requires special handling. I have not done this sort
} of biological TEM before and I would appreciate any tips or
} references.
}
} Thanks,
}
} Mark
}
} Login Host: 132.250.134.121
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Wed Dec 31 14:38:46 2008
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} mBVKcjqv024150
} 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 31 Dec 2008 14:38:45 -0600
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c58188c4a985-at-[206.69.208.22]}
} 8, 11 -- Date: Wed, 31 Dec 2008 14:38:45 -0600
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Stains for proteins
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================



==============================Original Headers==============================
18, 45 -- From prvs=Rosemary.White=245b0c6e9-at-csiro.au Thu Jan 1 02:28:37 2009
18, 45 -- Received: from vic-MTAout2.csiro.au (vic-MTAout2.csiro.au [150.229.64.38])
18, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n018Salr000397
18, 45 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2009 02:28:37 -0600
18, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
18, 45 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
18, 45 -- s=email; t=1230798516; x=1262334516;
18, 45 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
18, 45 -- mime-version:content-transfer-encoding:content-id:
18, 45 -- content-description:resent-date:resent-from:resent-sender:
18, 45 -- resent-to:resent-cc:resent-message-id:in-reply-to:
18, 45 -- references:list-id:list-help:list-unsubscribe:
18, 45 -- list-subscribe:list-post:list-owner:list-archive;
18, 45 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
18, 45 -- |Subject:=20Re:=20[Microscopy]=20viaWWW:=20Stains=20for
18, 45 -- =20proteins|Date:=20Thu,=201=20Jan=202009=2019:28:30=20+1
18, 45 -- 100|Message-ID:=20 {C582C9DE.254E%rosemary.white-at-csiro.au}
18, 45 -- |To:=20"twigg-at-estd.nrl.navy.mil"=20 {twigg-at-estd.nrl.navy.m
18, 45 -- il} |CC:=20 {microscopy-at-microscopy.com} |MIME-Version:=201.0
18, 45 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200812
18, 45 -- 312046.mBVKkI0o000869-at-ns.microscopy.com} ;
18, 45 -- bh=Qqw7PQ+4CGhNuJOW8npS3wA1KrnF+K90KFwey0QCEbs=;
18, 45 -- b=DqfzuClEsIyTsq37pUIArSoj7yXUOFidNhIa2e2ijQgl4Yywy5G81mwa
18, 45 -- w8GyYUsGJ8pJ2Nyb/im6Xu0zeA7lE8JW1D1Aud75R8VIrEfAc8eK6U6uB
18, 45 -- nSmXET/yLHObf4m;
18, 45 -- X-IronPort-AV: E=Sophos;i="4.36,312,1228050000";
18, 45 -- d="scan'208";a="6556498"
18, 45 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127])
18, 45 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 01 Jan 2009 19:28:34 +1100
18, 45 -- Received: from [152.83.167.123] (152.83.167.123) by
18, 45 -- exvic-htca02.nexus.csiro.au (138.194.81.127) with Microsoft SMTP Server id
18, 45 -- 8.1.311.2; Thu, 1 Jan 2009 19:28:31 +1100
18, 45 -- User-Agent: Microsoft-Entourage/12.10.0.080409
18, 45 -- Date: Thu, 1 Jan 2009 19:28:30 +1100
18, 45 -- Subject: Re: [Microscopy] viaWWW: Stains for proteins
18, 45 -- From: Rosemary White {rosemary.white-at-csiro.au}
18, 45 -- To: "twigg-at-estd.nrl.navy.mil" {twigg-at-estd.nrl.navy.mil}
18, 45 -- CC: {microscopy-at-microscopy.com}
18, 45 -- Message-ID: {C582C9DE.254E%rosemary.white-at-csiro.au}
18, 45 -- Thread-Topic: [Microscopy] viaWWW: Stains for proteins
18, 45 -- Thread-Index: AclriNeUwOTsbYjjT7C/5VQYoMWaywAYhVyZ
18, 45 -- In-Reply-To: {200812312046.mBVKkI0o000869-at-ns.microscopy.com}
18, 45 -- MIME-Version: 1.0
18, 45 -- Content-Type: text/plain; charset="US-ASCII"
18, 45 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================



From: dsherman-at-purdue.edu
Date: Thu, 1 Jan 2009 10:23:12 -0600
Subject: [Microscopy] Re: Developing EM class for undergrads + going digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kristin,

Developing a new class is always difficult but more so when you are still in
the process of setting up the lab. Regarding your digital question, we are
a major research facility that still uses primarily film. Our users come
from all areas of the university and have a very wide range of sample types
requiring an equally wide range of preparation techniques and original
magnifications. I have two equivalent TEMs with one having a 4k digital
camera. The camera is rarely used. Why?

For a number of reasons. One is that TEM is a very poor sampling technique.
We look at extremely small samples and then extrapolate to the whole. A
digital camera, especially a high-resolution bottom mounted one, captures a
very small area of the total screen. This makes sampling worse. Yes you
can reduce primary magnification to capture a larger area but then the
camera is doing the magnification rather than the TEM lenses. This is fine
for very low magnification but is not as desirable for higher mag. You can
take more digital images but this is time consuming and may not be as
informative as a single larger area.

Also, especially when dealing with samples that have a very wide grayscale
range, such as negatively stained samples where you often have intense dark
areas along with quite light ones, it is often much more difficult to
prevent oversaturation with a digital camera due to the enhanced contrast.
It often takes longer to get a good digital image as you try to find correct
camera settings than to take a negative. You have a hardcopy of your data
with a negative that then allows you to scan the negative at whatever
resolution value is desirable to get the enlargement you need without being
limited to the pixel size of the initial digital image.

Yes there are advantaged to going totally digital. Not having to deal with
expense of film and the time for the developing (~20min for 30-40negatives
plus some wash time) is preferable to some. You can check focus on the
screen and toss any image that is not just right. You need to be able to
accurately focus when using film to minimize loss (but I consider this a
part of learning to be a good microscopist). You need less beam exposure so
this can help with fragile samples. It is easier to work with low contrast
samples due to increased camera-provided contrast. I am sure there are many
other advantages as well, providing you can afford the initial significant
expense of purchasing the camera in the first place.

Our compromise is to teach students to recognize focus and learn to stigmate
at high magnification on the screen. Once they "get" this, they can then
focus and stigmate whenever and where ever needed with minimal time and
effort and get a very high percentage of usable negatives. Time on the scope
is normally less than when taking digital images so actual cost (scope time
+ negatives) is about equal. Students then scan their negatives at
resolution desired and have their digital images. We haven't printed at all
in about 4 years and only rarely for about 4 years previously to that.
Consumer scanners with transmitted light (under $700) do fine for this
purpose as do inkjet printers although students prefer to look at images on
screen and rarely print them.

There is certainly a place for digital cameras, especially when you are
taking low mag images and need to get results in a hurry such as in a
diagnostic situation. It is very helpful for the occasional user who has
problems focusing well and can be quite adequate/desirable for other needs,
such as electron diffraction, as you can get a good dynamic range. Just do
not feel that this is a must purchase for an undergraduate course when the
$$ can perhaps better go to other preparation equipment or supplies.

Regarding use of nitrogen...I admit that I am old school. Lots of samples
are very stable under the beam and contamination is not a hugh problem with
modern pumping systems...especially at low magnifications where reductions
in resolution due to contamination are not easily visible. But as soon as
we say that, someone will come along with a sample that is not stable and
you end up with a lot of contamination released into the column and adverse
effects as a result. Part of keeping a common facility up and running is to
train all users to do things in the same methodical manner. This greatly
reduces user error. I would rather err on the side of caution and require
everyone to use nitrogen than hope that students will recognize when it is
desirable. THAT WON"T HAPPEN!

My recommendation is to get two 25L dewars so that one can be off getting
filled while the other is available as needed. You will probably only have
to fill once every few weeks or so and hopefully there is a larger tank some
where on campus to make this possible.

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy




On 12/31/08 7:32 PM, "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi All,
}
} It's been a few years since I've been on the list server - doing those
} recommended postdocs to "broaden my horizons" beyond microscopy (I'm wondering
} if I should have followed that advice!).
}
} I'm gearing up to teach an EM class for biologists to undergrads. The class
} hasn't been taught at my university in eons, so I'm basically starting from
} scratch with a great set of brand new JEOL scopes and a lab full of virgin
} equipment. It would be a dream come true if not for the fact that I've got to
} learn how to use all of this brand new equipment in short order!
}
} I'm sure that I'll be in touch throughout the upcoming semester, but for now,
} I have a few questions on which I'd like your learned advice:
}
} 1. Does anyone have experience with a JEOL JEM1011 TEM? Thoughts on it? Do
} you have an abbreviated user's protocol that you would be willing to share? No
} one here has ever used this brand new (though 5 year old scope), and, though
} JEOL will graciously come and do a training session with me, I'd love any
} input you may have.
}
} 2. Going digital. We are looking at getting a digital image capture system.
} I'm old enough to have been trained in the darkroom long ago. Should I teach
} these students darkroom technique or just assume that they're in the digital
} age and go with either digital capture or digital image manipulation (scanning
} in EM negatives and printing)? Vote and let me know. And, if you've got a
} camera system, scanner or printer that you would recommend, that would be
} great.
}
} 3. Lastly, and this may show the old workhorse microscopes I was weaned on -
} LN2. I've been advised to just forget about using LN2 with this TEM for
} biological applications. Really? Granted, I'll talk to the JEOL rep about
} this, but if you've got thoughts on it, please share. I hear that getting LN2
} into our lab may be a problem, but my gut tells me that I should make ripples
} with this one.
}
} 4. Okay, one more, but it's an easy one. Does anyone have a recommendation for
} a quick and easy animal tissue to use for teaching? I'm a die hard plant
} person (perfusion - ahh!), but I think that not having experience handling and
} looking at animal tissue has been a handicap for me. I don't want that for my
} students. Can I just go pick up some chicken livers or something and not have
} to find something to sacrifice?
}
} That's it for now. I know that there will be more.
}
} Many thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
} k.lennon-at-frostburg.edu
}
}
}
}
} ==============================Original Headers==============================
} 13, 20 -- From kamlennon-at-yahoo.com Wed Dec 31 18:30:31 2008
} 13, 20 -- Received: from web84007.mail.mud.yahoo.com
} (web84007.mail.mud.yahoo.com [68.142.206.177])
} 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n010UVQW011058
} 13, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 31 Dec 2008 18:30:31 -0600
} 13, 20 -- Received: (qmail 23609 invoked by uid 60001); 1 Jan 2009 00:30:30
} -0000
} 13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 13, 20 -- s=s1024; d=yahoo.com;
} 13, 20 --
} h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Con
} tent-Type:Message-ID;
} 13, 20 --
} b=CqDVTUJAqH9OmwKIT05TcFhilN8W5b4TH4VVVw3XGiIuPorbXVxCisHm0it7iPSLaS9sjsY/Lhme
} JNa3JpJo/lOiIKMqBLserDqmQ1+3yFel3/IfzViKtTyyx4AUo6roq5dZ4iDp7nUrjnWr7aoXqkXozP
} M4YrBTri8ScUff9eU=;
} 13, 20 -- X-YMail-OSG:
} KuV9JQgVM1kqFtyeJQ5xhd81MO9MDtVbiXwLAuz7_j.epu5Np3DdPvW8kKX1n_x9ZA--
} 13, 20 -- Received: from [96.239.149.81] by web84007.mail.mud.yahoo.com via
} HTTP; Wed, 31 Dec 2008 16:30:30 PST
} 13, 20 -- X-Mailer: YahooMailWebService/0.7.247.3
} 13, 20 -- Date: Wed, 31 Dec 2008 16:30:30 -0800 (PST)
} 13, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 13, 20 -- Reply-To: kamlennon-at-yahoo.com
} 13, 20 -- Subject: Developing EM class for undergrads + going digital advice?
} 13, 20 -- To: Microscopy-at-Microscopy.com
} 13, 20 -- MIME-Version: 1.0
} 13, 20 -- Content-Type: text/plain; charset=us-ascii
} 13, 20 -- Message-ID: {295847.22686.qm-at-web84007.mail.mud.yahoo.com}
} ==============================End of - Headers==============================


==============================Original Headers==============================
18, 32 -- From dsherman-at-purdue.edu Thu Jan 1 10:23:12 2009
18, 32 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n01GNBma021014
18, 32 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2009 10:23:11 -0600
18, 32 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
18, 32 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n01GNAEQ002896;
18, 32 -- Thu, 1 Jan 2009 11:23:10 -0500
18, 32 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11])
18, 32 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n01GNASW018749;
18, 32 -- Thu, 1 Jan 2009 11:23:10 -0500
18, 32 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
18, 32 -- Thu, 1 Jan 2009 11:23:09 -0500
18, 32 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ;
18, 32 -- Thu, 1 Jan 2009 16:22:35 +0000
18, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119
18, 32 -- Date: Thu, 01 Jan 2009 11:22:34 -0500
18, 32 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going digital
18, 32 -- advice?
18, 32 -- From: Debby Sherman {dsherman-at-purdue.edu}
18, 32 -- To: {kamlennon-at-yahoo.com} , "message to: MSA list" {microscopy-at-microscopy.com}
18, 32 -- Message-ID: {C58257FA.2535C%dsherman-at-purdue.edu}
18, 32 -- Thread-Topic: [Microscopy] Developing EM class for undergrads + going digital
18, 32 -- advice?
18, 32 -- Thread-Index: AclsLSb5DjLGwKQiCUGlT29ZAf6ywQ==
18, 32 -- In-Reply-To: {200901010032.n010WP7K014214-at-ns.microscopy.com}
18, 32 -- Mime-version: 1.0
18, 32 -- Content-type: text/plain;
18, 32 -- charset="US-ASCII"
18, 32 -- Content-transfer-encoding: 7bit
18, 32 -- X-OriginalArrivalTime: 01 Jan 2009 16:23:09.0837 (UTC) FILETIME=[3C560BD0:01C96C2D]
18, 32 -- X-PMX-Version: 5.4.0.320885
18, 32 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 2 Jan 2009 06:12:30 -0600
Subject: [Microscopy] Re: viaWWW: Stains for proteins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm sure the government was regulations and requirements which make using
osmium tetroxide difficult, but like most chemical, osmium tetroxide can be
safely used. At rubber company in Akron we used a water dilution of osmium
tetroxide both as vapor phase to stain thin sections of unsaturated rubber
and added it to latex rubber has a hardener. We worked in a chemical hood,
wore thin nitrile rubber gloves used normal chemical practices and never
experienced a problem.

The vials crystal osmium were scored, broken and dropped into distilled
water (we used a taped wrapped bottle to protect the solution from light)
and small amounts were removed from the bottle with clean pasture pipets.
The used solutions were decanted into a open wide mouth bottle stored in
the back of the hood. The water evaporated, the osmium reacted with dust
and organic material in the air and once a year we properly discarded the
sludge. We also placed the used TEM grids, used vials and latex solutions
in that bottle.

If you get the results you want from other stains that's great. But don't
let scary internet precautions and many of the warnings we read persuade
you from using chemicals.

Too often, people with limited chemical experience and education make
difficult rules not to protect you, but to protect the organization from
imagined legal complications. These people are not charged with solving
the problems your are responsible for and have limited if any sympathy if
you are unable to achieve these goals due to their restrictions.



twigg-at-estd.nrl.na
vy.mil
To
12/31/2008 03:48 frank_karl-at-lincolnelectric.com
PM cc

Subject
Please respond to [Microscopy] viaWWW: Stains for
twigg-at-estd.nrl.na proteins
vy.mil












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both twigg-at-estd.nrl.navy.mil as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: Naval Research Laboratory

Title-Subject: [Filtered] Stains for proteins

Question: I have a new project which may entail staining proteins so
that there is more contrast for TEM imaging. One collaborator
recommended osmium tetroxide, but I read on the web that it is rather
poisonous and requires special handling. I have not done this sort
of biological TEM before and I would appreciate any tips or
references.

Thanks,

Mark

Login Host: 132.250.134.121
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Dec 31 14:38:46 2008
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id mBVKcjqv024150
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 31 Dec 2008
14:38:45 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c58188c4a985-at-[206.69.208.22]}
8, 11 -- Date: Wed, 31 Dec 2008 14:38:45 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Stains for proteins
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
27, 22 -- From frank_karl-at-lincolnelectric.com Fri Jan 2 06:12:27 2009
27, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
27, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02CCRJv027990
27, 22 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2009 06:12:27 -0600
27, 22 -- In-Reply-To: {200812312048.mBVKmOhZ004457-at-ns.microscopy.com}
27, 22 -- Subject: Re: [Microscopy] viaWWW: Stains for proteins
27, 22 -- To: twigg-at-estd.nrl.navy.mil, Microscopy-at-microscopy.com
27, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
27, 22 -- Message-ID: {OF639C253E.DA709F59-ON85257532.0040B9C6-85257532.00430824-at-lincolnelectric.com}
27, 22 -- Date: Fri, 2 Jan 2009 07:12:12 -0500
27, 22 -- From: Frank_Karl-at-lincolnelectric.com
27, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM,
27, 22 -- CD-MIME complete at 01/02/2009 07:12:13 AM,
27, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM,
27, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM,
27, 22 -- Serialize complete at 01/02/2009 07:12:13 AM
27, 22 -- MIME-Version: 1.0
27, 22 -- Content-Type: text/plain;
27, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 2 Jan 2009 06:37:14 -0600
Subject: [Microscopy] Re: Developing EM class for undergrads + going digital advice?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


2. Going digital. We are looking at getting a digital image capture system.
I'm old enough to have been trained in the darkroom long ago. Should I
teach these students darkroom technique or just assume that they're in the
digital age and go with either digital capture or digital image
manipulation (scanning in EM negatives and printing)? Vote and let me know.
And, if you've got a camera system, scanner or printer that you would
recommend, that would be great.

Hi Kristen,
I've used glass plates, cut film and digital imaging. I love the darkroom
work as well as the incredible images one gets from printing negatives with
the right "hardness" of paper, developer time and chemistry. With true
dedication you could take 20 images with the TEM, drop the vacuum, remove
the cassette, replace the cassette with a pre-pumped one (to lower the
water content and make your TEM pump down faster). If the negative
chemistry was at the proper temperature and not used up, you could develop,
dry the negative, then calibrate the enlarger, make sure the paper
chemistry was hot and not used up, determine the correct exposure, (it
always seemed to vary from negative to negative), print, develop, then
repeat with dodging or burning the image to bring out the details you need,
dry the paper, (used RC paper so you don't need a print dryer) and in eight
hours you could have two or three exceptional copies of each of the 20
negatives. (OH! I forgot make sure you record and identify the negatives
and store them so in twenty years someone will have several 100 pounds of
polyester film to dispose of.)

Or you could focus the microscope, move a lever or two, capture the image,
print it on a quality printer, decide if you want the image, correct the
exposure and composition and take another images. Yes, they are not as
nice as "photographs". But with the right paper, and a quality camera
(that means lots of bucks, pounds or yen) you can get an images 99% as good
and all in less then 3 minutes.

Digital, I dislike it, but it is the way to go........,

Stay safe........
Frank Karl.......
microscopist and former darkroom junkie

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
8, 23 -- From frank_karl-at-lincolnelectric.com Fri Jan 2 06:37:14 2009
8, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02CbCiG015828
8, 23 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2009 06:37:13 -0600
8, 23 -- In-Reply-To: {200901010037.n010ba2u023805-at-ns.microscopy.com}
8, 23 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going digital advice?
8, 23 -- Sensitivity:
8, 23 -- To: kamlennon-at-yahoo.com, Microscopy-at-microscopy.com
8, 23 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
8, 23 -- Message-ID: {OF1BAFA938.E4D83FCB-ON85257532.00432A06-85257532.004548CB-at-lincolnelectric.com}
8, 23 -- Date: Fri, 2 Jan 2009 07:36:48 -0500
8, 23 -- From: Frank_Karl-at-lincolnelectric.com
8, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM,
8, 23 -- CD-MIME complete at 01/02/2009 07:36:49 AM,
8, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM,
8, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM,
8, 23 -- Serialize complete at 01/02/2009 07:36:49 AM
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="US-ASCII"
==============================End of - Headers==============================




From: Rod-at-RJAndA.com
Date: Fri, 2 Jan 2009 09:25:56 -0600
Subject: [Microscopy] viaWWW: JEOL top reference holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Rod-at-RJAndA.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Rod-at-RJAndA.com
Name: Rod Johnson

Organization: Rod Johnson & Associates, Inc.

Title-Subject: [Filtered] JEOL top reference holders

Question: Happy New Year!

We purchased a used JEOL 840 this year. We are looking for 1 1/4
inch top reference holders. If you have holders you are no longer
using we would be pleased to pay for them and their shipping.

Regards,

Rod



Login Host: 24.177.172.69
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Jan 2 09:25:56 2009
11, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02FPsJZ001866
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2009 09:25:55 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c583e271b115-at-[206.69.208.22]}
11, 11 -- Date: Fri, 2 Jan 2009 09:25:51 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: Rod-at-RJAndA.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: JEOL top reference holders
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Fri, 2 Jan 2009 16:20:47 -0600
Subject: [Microscopy] January 2009 Microscopy Today Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the January 2009 Microscopy Today table of contents. We will
close the subscription list for this issue on Wednesday, January 7, 2009.
Microscopists in North America and MSA members anywhere qualify for free
subscriptions. Anyone else may subscribe for US$60 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com .

Thank you,
Ron Anderson, Technical Editor
=====================
Tiny Bubbles
Stephen W. Carmichael, Mayo Clinic

New Large Area Silicon Drift Detectors - Fast Analysis without Compromise
Clair Collins, Neil Rowlands, Peter Statham, and James Holland, Oxford
Instruments, High Wycombe, Bucks, England

Microscopy Today New Publication Directions
Ron Anderson and Charles Lyman,*Microscopy Today, Largo, FL and *Lehigh
University Bethlehem, PA

Manufacturer Training of Electron Microscopy and Analysis Techniques
Neil Rowlands, Oxford Instruments, Concord, MA

Remote Microscopy for Education and Outreach
S. Seraphin, S. Hernandez, G. Chandler, D. Bentley*, K. Dorame, M.
Sellers,** Univ. of Arizona, Tucson, AZ, * ** N. Arizona Univ.,
Flagstaff, AZ

The Electron Microscopy Database: an Online Resource for Teaching and
Learning Quantitative Transmission Electron Microscopy
Paul M. Voyles, Department of Materials Science and Engineering,
University of Wisconsin, Madison, Madison, WI

SEM Short Courses for Industry: the Lehigh Microscopy School as an example
Charles E. Lyman, Department of Materials Science and Engineering,
Lehigh University, Bethlehem, PA

Direct Visualisation, Sizing and Counting of Virus and Phage Particles
in Liquids
Bob Carr, and Duncan Griffiths,* NanoSight Ltd., Salisbury, UK,
*NanoSight USA, Costa Mesa, CA

Pioneers in Optics: Ernst Abbe (1840-1905)
Michael W. Davidson, The Florida State Univ., Tallahassee, FL

Single-Molecule DNA Stretching Using Optical Tweezers
Joost van Mameren, Anna Wozniak, and Sid Ragona,* JPK Instruments,
Berlin, Germany, *Ragona Scientific, Pittsford, NY

Event Streamed Spectrum Imaging using Programmed Beam Acquisition in
Biological Microprobe Analysis
P. Ingram,* S. D. Davilla,** & A. LeFurgey*, *Duke Univ. and Veterans
Affairs Med. Ctr, Durham, NC, **4pi Analysis Inc., Durham, NC

RGB-Splitting and Multi-Shot Techniques in Digital
Photomicrography–Utilization of Astronomic RGB-Filters in True Color
Imaging
Jörg Piper, Clinic “Meduna,” Bad Bertrich, Germany

Preventing the Sale of Fraudulent Gemstones using Non-Destructive X-Ray
Fluoresence Spectroscopy
Mary S. Goldman, Dan L. Davis, Robert H. Clifford, Shimadzu Scientific
Instruments Inc., Columbia, MD

Industry News

NetNotes
SPECIMEN PREPARATION - glutaraldehyde shelf life
SPECIMEN PREPARATION – Spurr’s resin
SPECIMEN PREPARATION – processing paraffin specimens for TEM
MICROTOMY – flattening sections
MICROTOMY – wetting the knife
MICROTOMY - coated grids
IMMUNOCYTOCHEMISTRY - fluorescence quenching
IMAGE PROCESSING - reference image subtraction
TEM – image distortion
TEM – comparison with STEM
SEM – backscattering detector image formation
SEM – backscatter detector
SEM - Coating for focused ion beam (FIB) SEM
SEM – active and passive acquisition
EM - SF6 detector
EM - CTF function
EM – pump speed vs. ultimate pressure
EM - plasma cleaner
SEM – oil shale rock sample preparation
SEM - of paper

Dear Abbe

Advertiser's Index


==============================Original Headers==============================
20, 17 -- From randerson20-at-tampabay.rr.com Fri Jan 2 16:20:44 2009
20, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02MKiqJ029455
20, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 2 Jan 2009 16:20:44 -0600
20, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
20, 17 -- by hrndva-omta01.mail.rr.com with ESMTP
20, 17 -- id {20090102222042.RAZV6232.hrndva-omta01.mail.rr.com-at-[127.0.0.1]}
20, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 2 Jan 2009 22:20:42 +0000
20, 17 -- Message-ID: {495E9335.8050806-at-tampabay.rr.com}
20, 17 -- Date: Fri, 02 Jan 2009 17:20:37 -0500
20, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
20, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
20, 17 -- MIME-Version: 1.0
20, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
20, 17 -- Subject: January 2009 Microscopy Today Table of Contents
20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed
20, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: Hobie-at-technicalsalessolutions.com
Date: Sat, 3 Jan 2009 13:21:10 -0600
Subject: [Microscopy] Extra Lab Items

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fellow Microscopy Listers,

We have the following equipment that we would like to find new homes for:
Lynx EL Tissue Processor and supplies
Digital Dryer (brand new, table top)
Arkay CD ­20 dryer
2-Codonics NP 1600 printers (brand new with supplies)

Photos are available upon request.

Priced as a donation to Valley Catholic High School EM Lab and shipping
costs, take one or all!

Thank you,

Hobie

Hobie Richards
Partner, and COO
Technical Sales Solutions, LLC
Portland, OR USA
www.TechnicalSalesSolutions.com
503 781 0428

Skype Hobie-TSS





==============================Original Headers==============================
11, 21 -- From Hobie-at-technicalsalessolutions.com Sat Jan 3 13:21:10 2009
11, 21 -- Received: from host203.com (host203.com [203.194.159.243])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n03JL8xC008261
11, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 13:21:09 -0600
11, 21 -- Received: (qmail 8983 invoked by uid 503); 3 Jan 2009 19:21:05 -0000
11, 21 -- Received: from unknown (HELO ?10.0.1.195?) (Hobie-at-76.115.10.232)
11, 21 -- by host203.com with ESMTPA; 3 Jan 2009 19:21:05 -0000
11, 21 -- User-Agent: Microsoft-Entourage/12.0.0.071130
11, 21 -- Date: Sat, 03 Jan 2009 11:20:58 -0800
11, 21 -- Subject: Extra Lab Items
11, 21 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com}
11, 21 -- To: {Microscopy-at-microscopy.com}
11, 21 -- Message-ID: {C584FA9A.16024%Hobie-at-technicalsalessolutions.com}
11, 21 -- Thread-Topic: Extra Lab Items
11, 21 -- Thread-Index: AcltJOhHkhtkCvXFM0ae5WxF7iwr5wAAVa5wACyKOCs=
11, 21 -- In-Reply-To: {C583CFB3.15FF3%Hobie-at-technicalsalessolutions.com}
11, 21 -- Mime-version: 1.0
11, 21 -- Content-type: text/plain;
11, 21 -- charset="ISO-8859-1"
11, 21 -- Content-Transfer-Encoding: 8bit
11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n03JL8xC008261
==============================End of - Headers==============================




From: as-at-astonmet.com
Date: Sat, 3 Jan 2009 14:52:49 -0600
Subject: [Microscopy] Cleaning House......Tracor Northern Parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tracor Northern:
PAC Control Console Joystick/Keypad
MicroScan Trackball/Slide Controller
2) Programmable Auto Controllers (steel boxes with boards)
Connector Box


We came across the above surplus items we acquired and never used. Not
wanting to throw these out if anyone can use them, we will happily box them
up for you. They will be yours for the cost of shipping.

Alan Stone
ASTON Metallurgical Services Co., Inc.
Wheeling, IL


==============================Original Headers==============================
6, 19 -- From as-at-astonmet.com Sat Jan 3 14:52:49 2009
6, 19 -- Received: from outbound1.mail.tds.net (outbound1.mail.tds.net [216.170.230.91])
6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n03Kqm16023434
6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:49 -0600
6, 19 -- Received: from outaamta01.mail.tds.net (outaamta01.mail.tds.net [216.170.230.31])
6, 19 -- by outbound1.mail.tds.net (8.13.6/8.13.4) with ESMTP id n03KqlMP023000
6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:47 -0600
6, 19 -- Received: from OFFICE.astonmet.com ([69.128.246.226])
6, 19 -- by outaamta01.mail.tds.net with ESMTP
6, 19 -- id {20090103205247.VXVJ26415.outaamta01.mail.tds.net-at-OFFICE.astonmet.com}
6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:47 -0600
6, 19 -- Message-Id: {6.2.0.14.2.20090103144053.02423dd0-at-pop.tds.net}
6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14
6, 19 -- Date: Sat, 03 Jan 2009 14:51:33 -0600
6, 19 -- To: microscopy-at-microscopy.com
6, 19 -- From: Alan Stone {as-at-astonmet.com}
6, 19 -- Subject: Cleaning House......Tracor Northern Parts
6, 19 -- Mime-Version: 1.0
6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: opmills-at-mtu.edu
Date: Sat, 3 Jan 2009 18:51:13 -0600
Subject: [Microscopy] viaWWW: wanted - 4pi SE II electronics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: opmills-at-mtu.edu
Name: Owen Mills

Organization: Michigan Technological University

Title-Subject: [Filtered] wanted - 4pi SE II electronics

Question: We'd like to buy (a donation would not hurt my feelings :} )
a 4pi SE II x-ray system. I really only need the control box, PCI
slot card and cables since we have a working detector and HV power
supply. Please contact me off-line at opmills-at-mtu.edu.

Thanks,

Owen Mills
Mich Tech Univ


Login Host: 24.236.189.168
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Sat Jan 3 18:51:13 2009
9, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n040pBNm021796
9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 18:51:12 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c585b869d98c-at-[206.69.208.22]}
9, 11 -- Date: Sat, 3 Jan 2009 18:51:07 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: wanted - 4pi SE II electronics
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hubner-at-iod.krakow.pl
Date: Mon, 5 Jan 2009 05:48:55 -0600
Subject: [Microscopy] conference Thermec 2009 Berlin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

International Conference on PROCESSING & MANUFACTURING OF ADVANCED MATERIALS
Processing, Fabrication, Properties, Applications
August 25-29, 2009, Technical University-Berlin, Germany
www.thermec.uow.edu.au

best regards
KJ Hübner


____________
Virus checked by G DATA AntiVirus
Version: AVF 19.204 from 29.12.2008
Virus news: www.gdata.pl


==============================Original Headers==============================
5, 26 -- From hubner-at-iod.krakow.pl Mon Jan 5 05:48:55 2009
5, 26 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n05BmsD2031012
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 05:48:55 -0600
5, 26 -- Received: from SERWER_SQL (serwer_sql [149.156.29.202])
5, 26 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id n05BRhvs015278
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 12:27:43 +0100 (CET)
5, 26 -- Received: from [149.156.29.4] (helo=iodkh007)
5, 26 -- by SERWER_SQL with AVK MailGateway;
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 05 Jan 2009 12:50:21 +0100
5, 26 -- Date: Mon, 5 Jan 2009 12:48:53 +0100
5, 26 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl}
5, 26 -- To: "Microscopy list" {microscopy-at-microscopy.com}
5, 26 -- MIME-Version: 1.0
5, 26 -- Message-ID: {8378428096D94ACBA990809C5149F148-at-iodkh007}
5, 26 -- Subject: conference Thermec 2009 Berlin
5, 26 -- Content-Type: text/plain;
5, 26 -- charset="iso-8859-2";
5, 26 -- format="flowed";
5, 26 -- reply-type="original"
5, 26 -- Content-Transfer-Encoding: 8bit
5, 26 -- X-Priority: 3
5, 26 -- X-MSMail-Priority: Normal
5, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
5, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
5, 26 -- X-AVK-Virus-Check: AVF 19.204;29.12.2008
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 5 Jan 2009 10:12:43 -0600
Subject: [Microscopy] Re: Developing EM class for undergrads + going

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kristen,

Hope this isn't too late - CMU shuts down over Christmas.

Good luck with the new class. Undergrads can be much better at things
like EM than people give them credit for (from experience with our EM
classes).
I can't help you with the JEOL, we have a Philips, but more generally:

2. Do both. Shoot film negatives and digital. Debby Sherman has
already provided excellent reasons for keeping the film negatives, so
I won't repeat those. I think it's worthwhile doing digital imaging
in addition because first, many labs are going that way, and you have
an opportunity to train students in the proper use of a digital
system, second, as Debby mentioned, it's cheaper to operate (no
chemicals, etc.) and so a useful system for teaching focus and
stigmation, and for quick checks of sections. If this is an either-or
decision, keep the film. The digital system can be learned whereever
the students go.

4. Crickets. We use cricket tissues in our TEM class. The femur
provides plenty of muscle, which has lots of interesting and easily
identifiable structures. Plus, it allows some basic study of
structure/ function, something that is missing from many microscopy
classes and shouldn't be. Fix the tissue in contracted and stretched
conditions, for example.
Further, there are lots of other interesting tissues: midgut, ventral
nerve cord, brain, Malphigian tubules, and so on. Also, since many
sections will contain tracheoles, there is another chance to discuss
physiology and microanatomy.

Phil

} Hi All,
}
} It's been a few years since I've been on the list server - doing
} those recommended postdocs to "broaden my horizons" beyond
} microscopy (I'm wondering if I should have followed that advice!).
}
} I'm gearing up to teach an EM class for biologists to undergrads.
} The class hasn't been taught at my university in eons, so I'm
} basically starting from scratch with a great set of brand new JEOL
} scopes and a lab full of virgin equipment. It would be a dream come
} true if not for the fact that I've got to learn how to use all of
} this brand new equipment in short order!
}
} I'm sure that I'll be in touch throughout the upcoming semester, but
} for now, I have a few questions on which I'd like your learned
} advice:
}
} 1. Does anyone have experience with a JEOL JEM1011 TEM? Thoughts on
} it? Do you have an abbreviated user's protocol that you would be
} willing to share? No one here has ever used this brand new (though 5
} year old scope), and, though JEOL will graciously come and do a
} training session with me, I'd love any input you may have.
}
} 2. Going digital. We are looking at getting a digital image capture
} system. I'm old enough to have been trained in the darkroom long
} ago. Should I teach these students darkroom technique or just assume
} that they're in the digital age and go with either digital capture
} or digital image manipulation (scanning in EM negatives and
} printing)? Vote and let me know. And, if you've got a camera system,
} scanner or printer that you would recommend, that would be great.
}
} 3. Lastly, and this may show the old workhorse microscopes I was
} weaned on - LN2. I've been advised to just forget about using LN2
} with this TEM for biological applications. Really? Granted, I'll
} talk to the JEOL rep about this, but if you've got thoughts on it,
} please share. I hear that getting LN2 into our lab may be a problem,
} but my gut tells me that I should make ripples with this one.
}
} 4. Okay, one more, but it's an easy one. Does anyone have a
} recommendation for a quick and easy animal tissue to use for
} teaching? I'm a die hard plant person (perfusion - ahh!), but I
} think that not having experience handling and looking at animal
} tissue has been a handicap for me. I don't want that for my
} students. Can I just go pick up some chicken livers or something and
} not have to find something to sacrifice?
}
} That's it for now. I know that there will be more.
}
} Many thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
} k.lennon-at-frostburg.edu

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
8, 26 -- From oshel1pe-at-cmich.edu Mon Jan 5 10:12:43 2009
8, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n05GCh4d019121
8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 10:12:43 -0600
8, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
8, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n05GCa96007385
8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 11:12:42 -0500
8, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
8, 26 -- Mon, 5 Jan 2009 11:12:19 -0500
8, 26 -- Mime-Version: 1.0
8, 26 -- Message-Id: {f06240808c587debf7f93-at-[141.209.160.249]}
8, 26 -- In-Reply-To: {200901010034.n010Y3AT016917-at-ns.microscopy.com}
8, 26 -- References: {200901010034.n010Y3AT016917-at-ns.microscopy.com}
8, 26 -- Date: Mon, 5 Jan 2009 11:12:17 -0500
8, 26 -- To: Microscopy-at-microscopy.com
8, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
8, 26 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going
8, 26 -- digital advice?
8, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 26 -- X-OriginalArrivalTime: 05 Jan 2009 16:12:19.0226 (UTC) FILETIME=[6231AFA0:01C96F50]
8, 26 -- X-Canit-CHI2: 0.00
8, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
8, 26 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
8, 26 -- X-CanItPRO-Stream: default
8, 26 -- X-Canit-Stats-ID: 7064876 - 02ca6d00f197
8, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: matsumot-at-lifesci.ucsb.edu
Date: Mon, 5 Jan 2009 12:56:53 -0600
Subject: [Microscopy] Course announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is an introductory digital microscopy workshop at UCSB (February
-13, 2009). If interested, please check the web site below:

https://www.lifesci.ucsb.edu/mcdb/events/imaging_workshop/index.php

==============================Original Headers==============================
2, 21 -- From matsumot-at-lifesci.ucsb.edu Mon Jan 5 12:56:53 2009
2, 21 -- Received: from lifesci.lifesci.ucsb.edu (lifesci.ucsb.edu [128.111.226.5])
2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n05IupM9005799
2, 21 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 12:56:52 -0600
2, 21 -- Received: from matsumoto-mb.lifesci.ucsb.edu (matsumoto-mb.lifesci.ucsb.edu [128.111.209.183])
2, 21 -- (authenticated bits=0)
2, 21 -- by lifesci.lifesci.ucsb.edu (8.14.3/8.14.1) with ESMTP id n05Iui4i005722
2, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
2, 21 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 10:56:48 -0800 (PST)
2, 21 -- Message-ID: {496257EC.9070501-at-lifesci.ucsb.edu}
2, 21 -- Date: Mon, 05 Jan 2009 10:56:44 -0800
2, 21 -- From: Brian Matsumoto {matsumot-at-lifesci.ucsb.edu}
2, 21 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
2, 21 -- MIME-Version: 1.0
2, 21 -- To: microscopy-at-microscopy.com
2, 21 -- Subject: Course announcement
2, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
2, 21 -- Content-Transfer-Encoding: 7bit
2, 21 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0 (lifesci.lifesci.ucsb.edu [128.111.226.5]); Mon, 05 Jan 2009 10:56:48 -0800 (PST)
2, 21 -- X-Virus-Scanned: ClamAV version 0.94, clamav-milter version 0.94-exp on lifesci
2, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: meulia.1-at-osu.edu
Date: Tue, 6 Jan 2009 09:54:24 -0600
Subject: [Microscopy] antibodies for BiFC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are doing BiFC to study interaction of two nucleoporins in vivo
first and then at the em level using immunogold labeling. Does anyone
know, if there is a GFP antibody available that would only recognize
the two halves when assembled?
Thanks for your help.

Tea
--
***************************************
Tea Meulia, PhD
Research Scientists and Director
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************

==============================Original Headers==============================
5, 21 -- From meulia.1-at-osu.edu Tue Jan 6 09:54:23 2009
5, 21 -- Received: from defang19.it.ohio-state.edu (defang19.it.ohio-state.edu [128.146.216.133])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n06FsNR8008224
5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 09:54:23 -0600
5, 21 -- Received: from defang9.it.ohio-state.edu (defang9.it.ohio-state.edu [128.146.216.78])
5, 21 -- by defang19.it.ohio-state.edu (8.13.7/8.13.1) with ESMTP id n06FsMaT007775
5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 10:54:22 -0500
5, 21 -- Received: from [140.254.186.120] (dhcp-140-254-186-120.osuwireless.ohio-state.edu [140.254.186.120])
5, 21 -- by defang9.it.ohio-state.edu (8.13.7/8.13.1) with ESMTP id n06FsM4c027286
5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 10:54:22 -0500
5, 21 -- Mime-Version: 1.0
5, 21 -- Message-Id: {p06240807c5892d4e19f3-at-[140.254.186.120]}
5, 21 -- Date: Tue, 6 Jan 2009 10:57:16 -0500
5, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 21 -- From: Tea Meulia {meulia.1-at-osu.edu}
5, 21 -- Subject: antibodies for BiFC
5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 21 -- X-Spam-Score: 0.00 () [Tag at 4.50]
5, 21 -- X-CanItPRO-Stream: outbound
5, 21 -- X-Canit-Stats-ID: Bayes signature not available
5, 21 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.146.216.133
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Wed, 7 Jan 2009 13:35:53 -0600
Subject: [Microscopy] Dr. Doug Benson -may he rest in peace

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have just received this message from Ash Prabala, President and CEO of DVC company:

"It is with profound regret that I inform you that Doug Benson, a dear friend and colleague, passed away early this morning at the age of 56. It is very difficult to find a silver lining in this news, but given the tough odds that he faced, at least he was spared a prolonged battle with cancer. Also, several generations of his family were close by and I know that he was greatly comforted by their proximity, and by their warmth and love.

Prior to his pivotal role as Chief Scientist and Director at DVC, Doug was the founder of Inovision Corporation - a highly regarded industry leader in the development of image analysis software. Before that, he was a National Cancer Institute Fellow at the Baylor College of Medicine in Houston, Texas. Douglas obtained a Ph.D. in Biochemistry from North Carolina State University.

I know that there are many in the Life Sciences, Microscopy & Imaging communities who knew Doug well, either personally or through his academic, research and/or business affiliations. Please keep Doug, his wife Louise, and their family members in your thoughts. I feel a deep sense of personal loss and sorrow, but will always remember Doug as a warm, generous, funny, brilliant, caring friend and colleague. I will miss his sense of humor and the twinkle in his eyes when he would recount a joke or a personal anecdote.

Rest In Peace, Doug. You will be missed by all those who had the privilege of knowing you."

Sincerely,

Ash Prabala
President & CEO, DVC Company
10200 Highway 290 West, Austin, TX 78736
Ph: 512-301-9564 x306; Fax: 512-288-2961
E-mail: {mailto:ash-at-dvcco.com} ash-at-dvcco.com
www.dvcco.com




==============================Original Headers==============================
4, 18 -- From bfoster-at-mme1.com Wed Jan 7 13:35:53 2009
4, 18 -- Received: from smtp113.sbc.mail.mud.yahoo.com (smtp113.sbc.mail.mud.yahoo.com [68.142.198.212])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n07JZrw5023462
4, 18 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 13:35:53 -0600
4, 18 -- Message-Id: {200901071935.n07JZrw5023462-at-ns.microscopy.com}
4, 18 -- Received: (qmail 57605 invoked from network); 7 Jan 2009 19:35:53 -0000
4, 18 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-99.168.106.177 with login)
4, 18 -- by smtp113.sbc.mail.mud.yahoo.com with SMTP; 7 Jan 2009 19:35:52 -0000
4, 18 -- X-YMail-OSG: _QWXCe4VM1lwpUW4YfCBRH3wib83Q0BnboKNIU_Z9g4yTodBrKYPTKHNFSVrSOhRIDjyyFVfN6s2Af.1mVnWF6TwzWSrg7nswPVEBTtrSug9vF.XfW33aaNHoj28JIETto6ZJmbO6jiO2mG43ahyunprtVECA0A5ZxVSHUhb6e8iBFcZIM8I6UqMPUcq6Euigzh6Q4kIM1u.Mvmur4cVahUN.rYP
4, 18 -- X-Yahoo-Newman-Property: ymail-3
4, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
4, 18 -- Date: Wed, 07 Jan 2009 13:32:29 -0600
4, 18 -- To: microscopy-at-microscopy.com
4, 18 -- From: Barbara Foster {bfoster-at-mme1.com}
4, 18 -- Subject: Dr. Doug Benson -may he rest in peace
4, 18 -- Cc: Ash Prabala {ash-at-dvcco.com}
4, 18 -- Mime-Version: 1.0
4, 18 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: bfostermme-at-sbcglobal.net
Date: Wed, 7 Jan 2009 13:45:12 -0600
Subject: [Microscopy] Dr. Doug Benson -may he rest in peace

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have just received this message from Ash Prabala, President and CEO of DVC company:

"It is with profound regret that I inform you that Doug Benson, a dear friend and colleague, passed away early this morning at the age of 56. It is very difficult to find a silver lining in this news, but given the tough odds that he faced, at least he was spared a prolonged battle with cancer. Also, several generations of his family were close by and I know that he was greatly comforted by their proximity, and by their warmth and love.

Prior to his pivotal role as Chief Scientist and Director at DVC, Doug was the founder of Inovision Corporation - a highly regarded industry leader in the development of image analysis software. Before that, he was a National Cancer Institute Fellow at the Baylor College of Medicine in Houston, Texas. Douglas obtained a Ph.D. in Biochemistry from North Carolina State University.

I know that there are many in the Life Sciences, Microscopy & Imaging communities who knew Doug well, either personally or through his academic, research and/or business affiliations. Please keep Doug, his wife Louise, and their family members in your thoughts. I feel a deep sense of personal loss and sorrow, but will always remember Doug as a warm, generous, funny, brilliant, caring friend and colleague. I will miss his sense of humor and the twinkle in his eyes when he would recount a joke or a personal anecdote.

Rest In Peace, Doug. You will be missed by all those who had the privilege of knowing you."

Sincerely,

Ash Prabala
President & CEO, DVC Company
10200 Highway 290 West, Austin, TX 78736
Ph: 512-301-9564 x306; Fax: 512-288-2961
E-mail: {mailto:ash-at-dvcco.com} ash-at-dvcco.com
www.dvcco.com




==============================Original Headers==============================
4, 22 -- From bfostermme-at-sbcglobal.net Wed Jan 7 13:45:12 2009
4, 22 -- Received: from smtp103.sbc.mail.mud.yahoo.com (smtp103.sbc.mail.mud.yahoo.com [68.142.198.202])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n07JjB2O001987
4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 13:45:12 -0600
4, 22 -- Message-Id: {200901071945.n07JjB2O001987-at-ns.microscopy.com}
4, 22 -- Received: (qmail 91265 invoked from network); 7 Jan 2009 19:45:11 -0000
4, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
4, 22 -- s=s1024; d=sbcglobal.net;
4, 22 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Cc:Mime-Version:Content-Type;
4, 22 -- b=WCjpFcJuSTpRX37VFu8lbg55i1nK9JHBIeprD672KurTXXqCeMQ3nyhtaDBsSFMuyAny0m3xtRQOzVLiPrzhq3Tq8X7VAjksy0Oj4enDikuAgjJGaksfgZxjKKMMkLcjvTmE7US9aXMG/5R3J6dVpN8iO0zcwirIO5xutQqiC48= ;
4, 22 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login)
4, 22 -- by smtp103.sbc.mail.mud.yahoo.com with SMTP; 7 Jan 2009 19:45:11 -0000
4, 22 -- X-YMail-OSG: 0YAkGukVM1mC2F9P.xdD7ektOrgTdOZ.gLUATGKzoF9Qa9BDk3YUXK.VvRiCQtyyfa7OLOYbTw7aadUcZsX7AV7RtRDJw75EGnRwSfhsNBuC8FZpRUfDlkAKRhMeldqsJMDq5e.BTmcWtk.b6O44eyyhuHzKya84xhjIYQFghwaGvmD6ZC0nplBIsTCXD7NG9edayIANAuEQth8ReSRJqGiZ2kmw
4, 22 -- X-Yahoo-Newman-Property: ymail-3
4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
4, 22 -- Date: Wed, 07 Jan 2009 13:41:44 -0600
4, 22 -- To: microscopy-at-microscopy.com
4, 22 -- From: Barbara Foster {bfostermme-at-sbcglobal.net}
4, 22 -- Subject: Dr. Doug Benson -may he rest in peace
4, 22 -- Cc: Ash Prabala {ash-at-dvcco.com}
4, 22 -- Mime-Version: 1.0
4, 22 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: hale0007-at-mc.duke.edu
Date: Wed, 7 Jan 2009 15:15:09 -0600
Subject: [Microscopy] Electron Microscopy Job Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


NOTE: DO NOT REPLY TO THIS EMAIL. SEE BELOW FOR APPLICATION INFORMATION.

Electron Microscopy Position Available
Official Title: Molecular Technologist II, III, or IV--depending on
qualifications and work experience

Location: Duke University Medical Center, Durham, NC

Requirements: Training and experience in running and maintaining electron
microscopes, proficiency in cutting ultrathin sections and performing
negative
staining. Knowledge of scientific laboratory operation (making solutions,
ordering, typing results, keeping records, etc.). Clinical laboratory and
research experience are a plus.

Laboratory description: The work force consists of the director and 6 EM
technologists who perform pathology (~500 samples/year), virology (~1000
samples/year), and research work; 3 TEMs; 1 SEM; 7 ultramicrotomes?2 with
cryo attachments; plus ancillary specimen preparation equipment.

Job descriptions available at:
https://www.hr.duke.edu/
Click ?Jobs?
Under ?Job Descriptions? click ?Duke University Health System?
Under ?Browse by Job Title? click ?M?
At the bottom click ?Molecular Tech II, III, or IV? (Alternatively, to get
to here, copy and paste:
https://www.hr.duke.edu/jobs/descr_duhs/job_title.php?ID=M

These descriptions are generic, and not all jobs described within (e.g.,
histology) are required of the EM position.

EM Laboratory web site:
http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain

Send resume to:
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Laboratory
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710

Phone: 919 684-3452
Fax: 919 684-3265
Email: saram-at-duke.edu


==============================Original Headers==============================
12, 26 -- From hale0007-at-mc.duke.edu Wed Jan 7 15:15:08 2009
12, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LF8KF020849
12, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 15:15:08 -0600
12, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54])
12, 26 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n07LF6bC2248714
12, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
12, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 16:15:07 -0500
12, 26 -- Importance: Normal
12, 26 -- X-Priority: 3 (Normal)
12, 26 -- In-Reply-To:
12, 26 -- References:
12, 26 -- Subject: Electron Microscopy Job Available
12, 26 -- MIME-Version: 1.0
12, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu}
12, 26 -- To: Microscopy-at-microscopy.com
12, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release
12, 26 -- 8.0.2|August 07, 2008) at 01/07/2009 16:15:03,
12, 26 -- MIME-CD complete at 01/07/2009 16:15:03,
12, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release
12, 26 -- 8.0.2|August 07, 2008) at 01/07/2009 04:15:07 PM
12, 26 -- Message-ID: {OF916EE768.54463420-ON85257537.0074BC4E-85257537.0074BC73-at-notes.duke.edu}
12, 26 -- Date: Wed, 7 Jan 2009 16:15:04 -0500
12, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008
12, 26 -- Content-type: text/plain; charset=US-ASCII
12, 26 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201
==============================End of - Headers==============================




From: dcrippen-at-buckinstitute.org
Date: Wed, 7 Jan 2009 15:31:35 -0600
Subject: [Microscopy] RE: Uranyl Acetate handling, storage, and disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,

A relatively new person in our EHS dept has informed us that UA is a
strong gamma emitter and should be stored and disposed of in a stainless
steel containersAND used in a stainless steel hood (or with other proper
protection measures). This was a surprise to us as all former EHS staff
have told us that though it DOES need to be disposed of with other
radioactive waste, it can be used in a normal hood. We obviously want
to be as safe as possible.

Can you advise on any special handling procedures used for UA?

Many thanks in advance!

danielle

Buck Institute for Age Research



==============================Original Headers==============================
3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009
3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org [64.84.58.24])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LVZR8002117
3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35 -0600
3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 22 -- Content-class: urn:content-classes:message
3, 22 -- MIME-Version: 1.0
3, 22 -- Content-Type: text/plain;
3, 22 -- charset="US-ASCII"
3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal
3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800
3, 22 -- Message-ID: {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org}
3, 22 -- In-Reply-To: {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
3, 22 -- X-MS-Has-Attach:
3, 22 -- X-MS-TNEF-Correlator:
3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal
3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA==
3, 22 -- References: {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org}
3, 22 -- To: {Microscopy-at-Microscopy.Com}
3, 22 -- Content-Transfer-Encoding: 8bit
3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n07LVZR8002117
==============================End of - Headers==============================




From: thoward-at-unm.edu
Date: Wed, 7 Jan 2009 17:14:04 -0600
Subject: [Microscopy] Uranyl Acetate handling, storage, and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm curious - has this new EHS person done actual
measurements (comparing distance from source, shielding,
etc.) on the UA that is commonly used in EM labs, or are
they assuming that you have undepleted UA? Maybe I'm
mistaken, but I thought the UA we buy from the EM supply
companies is not as radioactive as "plain" UA. I know I've
tried to get counts off of it (with a gamma detector) & it
isn't very hot.

Tamara


On Wed, 7 Jan 2009 15:32:23 -0600
dcrippen-at-buckinstitute.org wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear List,
}
} A relatively new person in our EHS dept has informed us
} that UA is a
} strong gamma emitter and should be stored and disposed
} of in a stainless
} steel containersAND used in a stainless steel hood (or
} with other proper
} protection measures). This was a surprise to us as all
} former EHS staff
} have told us that though it DOES need to be disposed of
} with other
} radioactive waste, it can be used in a normal hood. We
} obviously want
} to be as safe as possible.
}
} Can you advise on any special handling procedures used
} for UA?
}
} Many thanks in advance!
}
} danielle
}
} Buck Institute for Age Research
}
}
}
} ==============================Original
} Headers==============================
} 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7
} 15:31:35 2009
} 3, 22 -- Received: from inverness.buckcenter.org
} (webmail.buckinstitute.org [64.84.58.24])
} 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n07LVZR8002117
} 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan
} 2009 15:31:35 -0600
} 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 3, 22 -- Content-class: urn:content-classes:message
} 3, 22 -- MIME-Version: 1.0
} 3, 22 -- Content-Type: text/plain;
} 3, 22 -- charset="US-ASCII"
} 3, 22 -- Subject: RE: Uranyl Acetate handling, storage,
} and disposal
} 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800
} 3, 22 -- Message-ID:
} {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org}
} 3, 22 -- In-Reply-To:
} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
} 3, 22 -- X-MS-Has-Attach:
} 3, 22 -- X-MS-TNEF-Correlator:
} 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage,
} and disposal
} 3, 22 -- Thread-Index:
} AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA==
} 3, 22 -- References:
} {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org}
} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org}
} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
} 3, 22 -- From: "Danielle Crippen"
} {dcrippen-at-buckinstitute.org}
} 3, 22 -- To: {Microscopy-at-Microscopy.Com}
} 3, 22 -- Content-Transfer-Encoding: 8bit
} 3, 22 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n07LVZR8002117
} ==============================End of -
} Headers==============================

***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

==============================Original Headers==============================
5, 25 -- From thoward-at-unm.edu Wed Jan 7 17:14:03 2009
5, 25 -- Received: from unm.edu (f5vs2.unm.edu [64.106.76.41])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07NE2Pt018391
5, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 17:14:02 -0600
5, 25 -- Received: from [192.168.1.34] (HELO unm.edu)
5, 25 -- by regor.unm.edu (CommuniGate Pro SMTP 4.3.6)
5, 25 -- with ESMTP id 147954974; Wed, 07 Jan 2009 16:14:01 -0700
5, 25 -- X-PMX-Host: Theta
5, 25 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, FROM_EDU_TLD 0, RDNS_NXDOMAIN 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, WEBMAIL_SOURCE 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_HTTP_RECEIVED 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0'
5, 25 -- X-PMX-Version: x1 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.1.7.230423
5, 25 -- Received: from [64.234.154.122] (account thoward-at-unm.edu)
5, 25 -- by theta.unm.edu (CommuniGate Pro WebUser 4.3.6)
5, 25 -- with HTTP id 28903545; Wed, 07 Jan 2009 16:14:00 -0700
5, 25 -- From: "Tamara A Howard" {thoward-at-unm.edu}
5, 25 -- Subject: Re: [Microscopy] RE: Uranyl Acetate handling, storage, and
5, 25 -- disposal
5, 25 -- To: dcrippen-at-buckinstitute.org, Microscopy-at-microscopy.com
5, 25 -- X-Mailer: CommuniGate Pro WebUser Interface v.4.3.6
5, 25 -- Date: Wed, 07 Jan 2009 16:14:00 -0700
5, 25 -- Message-ID: {web-28903545-at-theta.unm.edu}
5, 25 -- In-Reply-To: {200901072132.n07LWNVC003859-at-ns.microscopy.com}
5, 25 -- References: {200901072132.n07LWNVC003859-at-ns.microscopy.com}
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Type: text/plain; charset="ISO-8859-1"; format="flowed"
5, 25 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: reganhll-at-gmail.com
Date: Wed, 7 Jan 2009 17:23:15 -0600
Subject: [Microscopy] viaWWW: EM TOMOGRAPHY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both reganhll-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: reganhll-at-gmail.com
Name: JOhnson

Organization: URI

Title-Subject: [Filtered] EM TOMOGRAPHY

Question: I am looking for a book on basics of EM tomograpyh.
Though i am into EM since last 5yrs ...Need to know about EM tomography.

Any suggestions



Login Host: 122.172.147.255
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Jan 7 17:23:14 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07NNEPx031982
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 17:23:14 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c58ae9d173ff-at-[206.69.208.22]}
9, 11 -- Date: Wed, 7 Jan 2009 17:23:12 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: reganhll-at-gmail.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: EM TOMOGRAPHY
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: abesenyo-at-ibilabs.com
Date: Wed, 7 Jan 2009 17:24:17 -0600
Subject: [Microscopy] viaWWW: Uranyl Acetate a Alpha Emitter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abesenyo-at-ibilabs.com
Name: Alex Besenyo PhD

Organization: President

Title-Subject: [Filtered] Uranyl Acetate a Alpha Emitter

Question: There is a posting that needs clarification.

As the sole world wide manufacturer of Uranyl Acetate and other
uranium compounds let me assure everyone that Uranyl Acetate is a
alpha emitter and not a gamma emmiter.

As for storage good house keeping rules apply.

If any one has any direct questions regarding this they can post or
contact me directly.

Alex Besenyo PhD




Login Host: 74.173.69.139
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Wed Jan 7 17:24:17 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07NOEer002161
13, 11 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 17:24:16 -0600
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240802c58aea017f53-at-[206.69.208.22]}
13, 11 -- Date: Wed, 7 Jan 2009 17:24:14 -0600
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: Uranyl Acetate a Alpha Emitter
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Wed, 7 Jan 2009 17:51:53 -0600
Subject: [Microscopy] RE: Uranyl Acetate handling, storage, and disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I worked with Uranium at ICN Pharmaceuticals and Isotope Products Labs
making standards. Uranium is an alpha emitter, which is far more deadly
internally. Also, Uranium is chemically toxic. So, keep it safe.

I doubt you'll find much dose coming off a small vial of U salt. Secondary
x-rays may be produced from the alpha-particles fluorescing the surrounding
matrix. Minimal shielding should suffice.



Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.

Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: dcrippen-at-buckinstitute.org [mailto:dcrippen-at-buckinstitute.org]
Sent: Wednesday, January 07, 2009 1:37 PM
To: dkloos-at-parallaxray.com

Dear List,

A relatively new person in our EHS dept has informed us that UA is a
strong gamma emitter and should be stored and disposed of in a stainless
steel containersAND used in a stainless steel hood (or with other proper
protection measures). This was a surprise to us as all former EHS staff
have told us that though it DOES need to be disposed of with other
radioactive waste, it can be used in a normal hood. We obviously want
to be as safe as possible.

Can you advise on any special handling procedures used for UA?

Many thanks in advance!

danielle

Buck Institute for Age Research



==============================Original Headers==============================
3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009
3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org
[64.84.58.24])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n07LVZR8002117
3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35
-0600
3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 22 -- Content-class: urn:content-classes:message
3, 22 -- MIME-Version: 1.0
3, 22 -- Content-Type: text/plain;
3, 22 -- charset="US-ASCII"
3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal
3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800
3, 22 -- Message-ID:
{69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org}
3, 22 -- In-Reply-To:
{69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
3, 22 -- X-MS-Has-Attach:
3, 22 -- X-MS-TNEF-Correlator:
3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal
3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA==
3, 22 -- References:
{69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org}
{69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org}
{69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org}
3, 22 -- To: {Microscopy-at-Microscopy.Com}
3, 22 -- Content-Transfer-Encoding: 8bit
3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n07LVZR8002117
==============================End of - Headers==============================


==============================Original Headers==============================
14, 30 -- From dkloos-at-parallaxray.com Wed Jan 7 17:51:53 2009
14, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07Nprki026992
14, 30 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 17:51:53 -0600
14, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
14, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
14, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
14, 30 -- id 1LKiBm-0000gH-5m; Wed, 07 Jan 2009 16:51:54 -0700
14, 30 -- Reply-To: {dkloos-at-parallaxray.com}
14, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
14, 30 -- To: {dcrippen-at-buckinstitute.org}
14, 30 -- Cc: {microscopy-at-microscopy.com}
14, 30 -- References: {200901072136.n07Laq35015255-at-ns.microscopy.com}
14, 30 -- Subject: RE: [Microscopy] RE: Uranyl Acetate handling, storage, and disposal
14, 30 -- Date: Wed, 7 Jan 2009 15:51:48 -0800
14, 30 -- Organization: Parallax Research
14, 30 -- Message-ID: {6735FBA29934490C8F3B6EB1137FF376-at-donl}
14, 30 -- MIME-Version: 1.0
14, 30 -- Content-Type: text/plain;
14, 30 -- charset="us-ascii"
14, 30 -- Content-Transfer-Encoding: 7bit
14, 30 -- X-Mailer: Microsoft Office Outlook 11
14, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
14, 30 -- In-Reply-To: {200901072136.n07Laq35015255-at-ns.microscopy.com}
14, 30 -- thread-index: AclxEA7jOgj41WzYT5aTp4V8xWeubQAEkOvQ
14, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
14, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
14, 30 -- X-AntiAbuse: Original Domain - microscopy.com
14, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
14, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: gantz-at-bu.edu
Date: Wed, 7 Jan 2009 18:10:14 -0600
Subject: [Microscopy] Defective Electron Image Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
Today we have encountered what appears to be defective Kodak SO-163
electron image film, 3 1/4 x 4 inch, catalog #8010100. Those of you who
use this film will know that when loading the film into cassettes, if the
notch in the film is at the upper right-hand corner, then the emulsion side
of the film is up. The suspected defective film has the emulsion side down
with the notch at the upper right.

In addition, normally these packets of film have a slight upward curl
(concave) with the emulsion side up and notch to the upper right. This
potentially defective film has a downward curl (convex) with the notch to
the upper right.

On the box of film which is a multipak of 250 sheets, the following
numbers are found on the label:

Emul No. 239 002 07
2010-08
00277396

We do not know how widespread this problem is but we recommend not to use
film of this lot number until more information from Kodak is available.

Mr. Donald Gantz
Research Histologist/Electron Microscopist
Dept. Physiology and Biophysics
Center for Advanced Biomedical Research
Boston University School of Medicine
700 Albany Street
Boston, MA 02118
email: gantz-at-bu.edu
phone: 617-638-4017


==============================Original Headers==============================
7, 25 -- From gantz-at-bu.edu Wed Jan 7 18:10:14 2009
7, 25 -- Received: from relay15.bu.edu (relay15.bu.edu [128.197.27.66])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n080AECu008276
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 18:10:14 -0600
7, 25 -- X-Envelope-From: gantz-at-bu.edu
7, 25 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu [155.41.208.134])
7, 25 -- by relay15.bu.edu (8.13.1/8.13.1) with ESMTP id n0809mVA013635;
7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500
7, 25 -- Received: from gantz (cabr3-dhcp-208-153.bumc.bu.edu [155.41.208.153])
7, 25 -- by biophysics1.bumc.bu.edu (8.13.8/8.13.8) with SMTP id n0809mF4015021;
7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500
7, 25 -- Message-ID: {000b01c97125$69b0c190$99d0299b-at-gantz}
7, 25 -- From: "Don Gantz" {gantz-at-bu.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- Cc: {bullitt-at-bu.edu}
7, 25 -- Subject: Defective Electron Image Film
7, 25 -- Date: Wed, 7 Jan 2009 19:09:45 -0500
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="iso-8859-1"
7, 25 -- Content-Transfer-Encoding: 7bit
7, 25 -- X-Priority: 3
7, 25 -- X-MSMail-Priority: Normal
7, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807
7, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Thu, 8 Jan 2009 03:42:35 -0600
Subject: [Microscopy] Uranyl Acetate handling, storage, and disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Danielle,

Uranium is a natural radioactive element which is "mainly" alpha
emitter. Uranium isotopes also have a very small probablity of
"spontaneous fission", as well (this is not the main concern here of
course).

Since, the decay product of Uranium is also radioactive, you will need
to follow the decay tree to get a better feeling of what kinds of
other elements/particles might be present in Uranyl Acetate.

You may be able to construct the decay tree using the information from

http://atom.kaeri.re.kr/ton/nuc7.html

It will be a tedious calculation to assess the health risk from
handling Uranyl Acetate. It is always best to opt on the side of more
shielding. Go with ALARA (As Low As Reasonably Achievable)
principles.


Hope this helps,
Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Wed, Jan 7, 2009 at 11:36 PM, {dcrippen-at-buckinstitute.org} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear List,
}
} A relatively new person in our EHS dept has informed us that UA is a
} strong gamma emitter and should be stored and disposed of in a stainless
} steel containersAND used in a stainless steel hood (or with other proper
} protection measures). This was a surprise to us as all former EHS staff
} have told us that though it DOES need to be disposed of with other
} radioactive waste, it can be used in a normal hood. We obviously want
} to be as safe as possible.
}
} Can you advise on any special handling procedures used for UA?
}
} Many thanks in advance!
}
} danielle
}
} Buck Institute for Age Research
}
}
}
} ==============================Original Headers==============================
} 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009
} 3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org [64.84.58.24])
} 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LVZR8002117
} 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35 -0600
} 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 3, 22 -- Content-class: urn:content-classes:message
} 3, 22 -- MIME-Version: 1.0
} 3, 22 -- Content-Type: text/plain;
} 3, 22 -- charset="US-ASCII"
} 3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal
} 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800
} 3, 22 -- Message-ID: {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org}
} 3, 22 -- In-Reply-To: {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
} 3, 22 -- X-MS-Has-Attach:
} 3, 22 -- X-MS-TNEF-Correlator:
} 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal
} 3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA==
} 3, 22 -- References: {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org}
} 3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org}
} 3, 22 -- To: {Microscopy-at-Microscopy.Com}
} 3, 22 -- Content-Transfer-Encoding: 8bit
} 3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n07LVZR8002117
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
11, 37 -- From celikaktas-at-gmail.com Thu Jan 8 03:42:35 2009
11, 37 -- Received: from mail-bw0-f12.google.com (mail-bw0-f12.google.com [209.85.218.12])
11, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n089gYUr001455
11, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 03:42:35 -0600
11, 37 -- Received: by bwz5 with SMTP id 5so19380550bwz.18
11, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08 Jan 2009 01:42:34 -0800 (PST)
11, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 37 -- d=gmail.com; s=gamma;
11, 37 -- h=domainkey-signature:received:received:message-id:date:from:to
11, 37 -- :subject:in-reply-to:mime-version:content-type
11, 37 -- :content-transfer-encoding:content-disposition:references;
11, 37 -- bh=F6/+OkqdT9cZx582DnuGX2hhNlDOcxiW8bm465X3GwY=;
11, 37 -- b=VnnH9eF0uwCJJE6/gxg76+P2WFKZqlCa8aBVMhkBg7S0juRa4LjX6VmWkQyPDYFOB5
11, 37 -- 3tC5DekzLSE18qDjMQK3GztI/p34iTeRd/9aFPQpX0S3AmiDY/hYMjD6KUJSYhbqyCjT
11, 37 -- k2JZMiEG7YQXTlJRWqNm7g9Ubl7HS7sEZNzNk=
11, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
11, 37 -- d=gmail.com; s=gamma;
11, 37 -- h=message-id:date:from:to:subject:in-reply-to:mime-version
11, 37 -- :content-type:content-transfer-encoding:content-disposition
11, 37 -- :references;
11, 37 -- b=CoV6WgH7zhYYPfrk+BCYBdXHTY9DPVsv+9IA7A/XwAbrl6RCvrmclEr7MClN5E9uiG
11, 37 -- l3BRTD4Qdlx92HtT8a3+Ii8Guqf681nJuLgJGUCTlxidqc9Lb2HKRAN7PZf1ft2MLWuk
11, 37 -- +HTZU8TLfkxU8cfISdieUXSMkUVH91kuqhX+U=
11, 37 -- Received: by 10.103.160.9 with SMTP id m9mr8665947muo.96.1231407697116;
11, 37 -- Thu, 08 Jan 2009 01:41:37 -0800 (PST)
11, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan 2009 01:41:37 -0800 (PST)
11, 37 -- Message-ID: {1075c5c10901080141m536a64cetd987982e6ea26ad1-at-mail.gmail.com}
11, 37 -- Date: Thu, 8 Jan 2009 11:41:37 +0200
11, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com}
11, 37 -- To: microscopy {Microscopy-at-microscopy.com}
11, 37 -- Subject: Re: [Microscopy] RE: Uranyl Acetate handling, storage, and disposal
11, 37 -- In-Reply-To: {200901072136.n07La7w4013160-at-ns.microscopy.com}
11, 37 -- MIME-Version: 1.0
11, 37 -- Content-Type: text/plain; charset=UTF-8
11, 37 -- Content-Transfer-Encoding: 7bit
11, 37 -- Content-Disposition: inline
11, 37 -- References: {200901072136.n07La7w4013160-at-ns.microscopy.com}
==============================End of - Headers==============================




From: Bruce.Ingber-at-ARS.USDA.GOV
Date: Thu, 8 Jan 2009 09:50:59 -0600
Subject: Re: Uranyl Acetate radiation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I've included a group of emails (not all) from September, 1998 on this
same discussion. We did measure beta and gamma emissions greater than
background levels from the uranyl acetate stock reagent bottles, not
from solutions.

It's still wise to use proper PPE methods especially when weighing and
preparing solutions. Store the reagent bottles inside/behind shielding
in a relatively secluded area and treat old solutions as heavy metal
waste. Don't pour down sinks!

Bruce F. Ingber
USDA-ARS, SRRC
Biologist/Electron Microscopy
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
Bruce.Ingber-at-ars.usda.gov

ph. 504-286-4270
fax 504-286-4217
cel 504-782-6323

-----Original Message-----
X-from: thoward-at-unm.edu [mailto:thoward-at-unm.edu]
Sent: Wednesday, January 07, 2009 5:21 PM
To: Ingber, Bruce

I'm curious - has this new EHS person done actual
measurements (comparing distance from source, shielding,
etc.) on the UA that is commonly used in EM labs, or are
they assuming that you have undepleted UA? Maybe I'm
mistaken, but I thought the UA we buy from the EM supply
companies is not as radioactive as "plain" UA. I know I've
tried to get counts off of it (with a gamma detector) & it
isn't very hot.

Tamara


On Wed, 7 Jan 2009 15:32:23 -0600
dcrippen-at-buckinstitute.org wrote:
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Dear List,
}
} A relatively new person in our EHS dept has informed us
} that UA is a
} strong gamma emitter and should be stored and disposed
} of in a stainless
} steel containersAND used in a stainless steel hood (or
} with other proper
} protection measures). This was a surprise to us as all
} former EHS staff
} have told us that though it DOES need to be disposed of
} with other
} radioactive waste, it can be used in a normal hood. We
} obviously want
} to be as safe as possible.
}
} Can you advise on any special handling procedures used
} for UA?
}
} Many thanks in advance!
}
} danielle
}
} Buck Institute for Age Research
-----Original Message-----
X-from: Warren E Straszheim [mailto:bingber.BAYOU.SRRCDOM]
Sent: Wednesday, September 23, 1998 4:13 PM
To: bingber.BAYOU.SRRCDOM


FWIW

Some years ago I heard an account of a radiation safety inspection as
part
of a larger safety review at a large lab. The lab was involved in coal
research, as were we, and had a fair amount of coal samples stored in
glass
jars. An inspection team came thru and happened to check the jars for
radiation, found some, and instructed the researchers that they would
need
to start following radiation safety procedures.

Now coal can contain minute amounts of uranium in its mineral matter,
but
not enough that should set off a detector. Besides the detectors were
setup
to measure alpha particles, and there was no way that alpha particles
emitted from the contents of a glass jar should be detected on the
outside.

A little digging revealed that there was in fact a little radiation
present, but it was a slight residue left on the jar surface after
washing.
Apparently the jars went through the same washer as did other jars which
had held radioactive materials. I think the radiation safety folks may
have
received additional training after the incident.

You can draw your own moral from the story.

Warren

} From: "Ingber, Bruce F." {bingber-at-commserver.srrc.usda.gov}
} Date: Tue, 22 Sep 1998 10:51:45 -0500

{snip}

} Anyway, while gathering all uranium compounds, our radiation safety
officer checked the compounds using a dosimeter that recorded alpha,
beta,
and gamma radiation. Levels of radiation were found that were just below
OSHA guidelines for maximum daily exposure when the powder was checked
from
several inches away from the dosimeter. Thus, we decided to store the
compounds in an acrylic box behind a beta shield in an isolated
location.
Whenever the actual Uranyl acetate is weighed to prepare dilute
solutions
proper safety precautions are recommended, i.e. use a beta shield, wear
gloves and dust mask, weigh in a low occupancy room, etc.
}
} We repeated our dosimeter readings last month with two different alpha,
beta, and gamma dosimeters taking readings several feet from the
bottles,
removing the two acrylic shields one by one, and then with the cover of
the
UAc exposed (always moving closer to the chemical source). I won't enter
the millirem/rad discussion; suffice to say it's an interesting little
experiment for your support staff especially with the dosimeters left on
audio signal.

{snip}

----------------------------------------------------
Warren E. Straszheim
23 Town Engineering
Iowa State University
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
http://www.marl.iastate.edu

electron microscopy, x-ray analysis, image analysis, computer
applications

-----Original Message-----
X-from: "Woody.N.White-at-mcdermott.com"-at-Sparc5.Microscopy.Com
[mailto:bingber.BAYOU.SRRCDOM]
Sent: Thursday, September 17, 1998 9:28 PM
To: bingber.BAYOU.SRRCDOM


This bounced once, I shall try again...
(Nestor ignore the (not spam) email if this makes it to the
listserver ok)
----------------------------------------------------------------

Well....

The Nuclear Regulatory Commission limits of skin & extremity
(hands, feet) is 50 Rem per year. ...Not something to "shoot" for,

since the dose is also limited to "As Low As Reasonably
Achievable".

The (damage) conversion coefficient from mR from this source (no
alpha if not ingested) to mRem is ~1. 50 Rem = 50,000 mRem. At a

dose rate of 0.6 mR/hr, one would have to hold the container for
many years to receive a one year maximum dose (50,000 / 0.6 per hr
= max hours exposure). At 5 mR/hr, it would be 50,000 / 5. At that

one would have to hold the container for 10,000 hours before
exceeding NRC dose limits. Exposure will also decrease as a
function of the square of the distance from the source.

For medical tests to discover any changes in body chemistry, it
would take about 50 Rem acute whole body exposure.

Less dose is always better, but in realistic terms the dose from
the UA should not be of any concern. If this level is of concern,
do not fly in airplanes, live at high elevations, avoid all medical

radiation, avoid certain beaches, beware of granite buildings, run
from radium dial watches, etc. :)

The real danger is if the UA enters the body where the alpha source

is in direct contact with livings tissue. Radiological bio-assay
(urine/fecal) would be required to detect this.

Woody White
McDermott Technology

-----Original Message-----
X-from: William Tivol [mailto:bingber.BAYOU.SRRCDOM]
Sent: Thursday, September 17, 1998 9:58 PM
To: bingber.BAYOU.SRRCDOM


Dear David,

} Using Bill's number's, you would
} still be well under the limits for occupational exposure if you were
in
} constant contact with .6 millirem/hr for a 2000 hr work year, (correct
me,
} but my references place the limits at 1.25 rem/quarter, 5 rem/year
whole
} body

These limits are for radiation workers. Because we get paid, we
can be exposed to a greater risk. The limit for the general population
is
0.5 rem/year whole body, and I think this limit also applies to women
who
are or may be pregnant. I do not know the status of graduate students;
I'd be inclined to err on the side of caution--especially since it is
fairly
easy to keep exposure to UA low.
Yours,
Bill Tivol
-----Original Message-----
X-from: David Bentley [mailto:bingber.BAYOU.SRRCDOM]
Sent: Thursday, September 17, 1998 8:09 PM
To: bingber.BAYOU.SRRCDOM


Responding with tidbits regarding this thread.

We make up a saturated stock bottle which we draw from, and
replenish
with UA and water (8-10g UA/100 ml) from time to time. The insoluble
material is described in the Merck Index as being due to insoluble basic
salts. It describes Uranyl Acetate as being "freely soluble in water
acidulated with acetic acid" For years, we have followed a modification
of
a procedure from Millonig's 1976 book Laboratory Manual of Biological
Electron Microscopy (pg 53) and added a few drops of acetic acid per
100mls
of stock saturated UA (stored in a brown bottle). This seems to push
the
ppt reaction the other way and give a clear solution. There seems to be
little difference in staining as long as only a few drops of acetic acid
are used. Changing the pH of the stain by much, is risky though as
there
are numerous papers and procedures which modify the effects of UA stain
by
doing so. We have raised the pH to the 4.5-5.5 (any higher and the UA
will
ppt) and gotten improved staining but with unacceptable amounts of ppt
on
the sections.

When compared with the other chemicals in the EM lab, UA would
seem to be
relatively safe when used carefully. Ingestion and inhalation (exposure
to
dust) are our major concern due to heavy metal toxicity as well as the
radiation hazards. Making sure that surfaces are not contaminated, and
cleaning any spillage immediately from bottles and tables before it
dries
are important steps. Wearing gloves, and hand washing after glove
removal
are also important safeguards.

The radiation exposure hazard under most operating conditions
seems
minimal. The least exposure possible is desirable (ALARA), when you
don't
need to handle it, don't be near it. Using Bill's number's, you would
still be well under the limits for occupational exposure if you were in
constant contact with .6 millirem/hr for a 2000 hr work year, (correct
me,
but my references place the limits at 1.25 rem/quarter, 5 rem/year whole
body and 18.75 rem/quarter, 75 rem/year for extremities (Rayburn)) The
other factor to keep in mind is that we are not talking about a whole
body
exposure, but just exposure to the hands. All in all, the amount of
exposure while making up and staining grids seems miniscule.
-----Original Message-----
X-from: ROBIN CROSS [mailto:bingber.BAYOU.SRRCDOM]
Sent: Wednesday, September 16, 1998 7:10 AM
To: bingber.BAYOU.SRRCDOM


The concentration quoted is far higher (btw at what
concentration in water does UA become a saturated solution?)
than normally would be used for EM staining.

} A solution of 10g UA in 15mls H2O was measured with a Geiger counter.

I have been using UA for many years and I recall that whenever I
questioned and investigated its possible radiation implications
I have been assured that it is not dangerous at the
concentrations and quantities we use, provided that it is not
ingested.
Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za - tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/affiliates/emu/em.htm

As an aside, the pretty flowered dinnerware from the 50's, the
vivid
oranges and yellows are from uranium. If you have any, run a Geiger
counter over them, you'll be surprised the number of counts. Also the
mantles from gas and propane lanterns contain radioactive thorium. In
the
past health physicist have suggested using them(sealed in their bags)
for
check sources for counters.

Regardless, because of the toxicity, radiation hazard, as well
as expenses
to purchase(well over $1.00/gm) and dispose of UA, minimizing the amount
needed to be discarded and wasted seems desirable. To the extents
possible, use of minimal amounts, and if considerable staining is done,
making stock saturated solutions which can be diluted to the desired
concentration as needed, are good ways to conserve UA, minimize
radiation
exposure, and inhalation and ingestion hazards.

Now, if we are starting a poll for the chemicals in the EM Lab
that make
us the most anxious, my vote is for cacodylate.
-----Original Message-----
X-from: William Tivol [mailto:bingber.BAYOU.SRRCDOM]
Sent: Wednesday, September 16, 1998 4:34 PM
To: bingber.BAYOU.SRRCDOM


Dear Josephine,
}
} A solution of 10g UA in 15mls H2O was measured with a Geiger counter.
} 500
} counts/sec was generated.
} A supplier had measured 100g UA :-
} 1 Alpha - {2 counts/sec, using a 540 scintillation meter with AP-2
Probe
} 2 Beta - } 500 counts/sec, using a 540 E1 probe coupled to a GM Meter
(this
} determines beta events and some low energy gamma events)
} 3 Gamma dose Rate (energy field) - two measurements done:
} using Mini monitor type R with GM Probe - 0.6mR/hr (mainly gamma)
} and Ionization chamber DMM 95/0500 - 5 mR/hr (Beta and Gamma
energy
} field).
} 4 Specific Activity (U approx. 55%) = 1.04 x 10 { {...} } Bq { {...} } gm

} { {...} } .
}
I calculated approximately the expected activity from 30 g UA
(about 0.1 mole, or 6*10^22 atoms). T_1/2 is 4.4*10^9 y and there are
3.1*10^7 s/y, so the decay rate is 5*10^-18 s^-1, and the activity is
3*10^5 Bq.
The build-up of daughter products with shorter half-lives will
reach steady state at which point the activities of the daughters will
be
the same as that of the parent. Pa 234 has a gamma transition, and
there
are several betas in the chain. The longer-lived isotopes in the chain
have lives of 10^4 to 10^5 y, and these will not be at steady state
(unless
your UA is *very* old ;-) ). 0.6 mR/hr is a significant amount of
exposure,
and, if one were to hold the jars for some minutes, a sizable fraction
of
the allowed annual dose would be attained.

} Can UA be used openly without protection in laboratory?
}
Small amounts can be used, but be sure to wash hands before
eating.
One area of the lab should be used for UA. A quiet area with little
traffic
is best. UA, while not nearly the most dangerous EM reagent, should
still
be treated with respect.
Yours,
Bill Tivol


==============================Original Headers==============================
63, 31 -- From Bruce.Ingber-at-ARS.USDA.GOV Thu Jan 8 09:50:59 2009
63, 31 -- Received: from messagescreen3.ars.usda.gov (messagescreen3.ars.usda.gov [199.133.180.150])
63, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08FowRF024837
63, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 09:50:58 -0600
63, 31 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV (ars.usda.gov [199.133.183.227] (may be forged))
63, 31 -- by messagescreen3.ars.usda.gov (8.13.8/8.13.8) with ESMTP id n08FobJw024001
63, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 09:50:58 -0600
63, 31 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959);
63, 31 -- Thu, 8 Jan 2009 08:48:55 -0700
63, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
63, 31 -- Content-class: urn:content-classes:message
63, 31 -- MIME-Version: 1.0
63, 31 -- Content-Type: text/plain;
63, 31 -- charset="us-ascii"
63, 31 -- Subject: RE: [Microscopy] Uranyl Acetate handling, storage, and
63, 31 -- Date: Thu, 8 Jan 2009 08:48:55 -0700
63, 31 -- Message-ID: {8017F94146BF634DA9414E4B9088525B03E8D4D4-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV}
63, 31 -- X-MS-Has-Attach:
63, 31 -- X-MS-TNEF-Correlator:
63, 31 -- Thread-Topic: [Microscopy] Uranyl Acetate handling, storage, and
63, 31 -- Thread-Index: AclxHrQEWtl0gXUIQCyvDFYeWl60ygAg4LogAAGC4gA=
63, 31 -- References: {200901072321.n07NLLKA030038-at-ns.microscopy.com}
63, 31 -- From: "Ingber, Bruce" {Bruce.Ingber-at-ARS.USDA.GOV}
63, 31 -- To: {microscopy-at-microscopy.com}
63, 31 -- X-OriginalArrivalTime: 08 Jan 2009 15:48:55.0477 (UTC) FILETIME=[9CBBD650:01C971A8]
63, 31 -- X-MessageScreenMessageID: 1231429858.626943.1253.994197512
63, 31 -- X-MessageScreenContentScore: Score of 5 assigned to Content
63, 31 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE
63, 31 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm)
63, 31 -- Content-Transfer-Encoding: 8bit
63, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n08FowRF024837
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Thu, 8 Jan 2009 12:41:25 -0600
Subject: [Microscopy] Defective Electron Image Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mr. Gantz,
I have received boxes of Kodak 4489 film in the past that were marked wrong.
It is obvious in the darkroom when you are loading the film in the holders
that the emulsion was on the wrong side (or, alternatively, that they put
the notch in the wrong place). The emulsion is the brighter side, the back
is the dark side. This happened with two or three boxes, several years ago,
and I informed the Kodak company with their reply card that comes with the
film. I received no reply.
I used the film, with the emulsion side up, as usual and it was just fine.
The emulsion makes the plastic curl slightly towards it, but it usually
flattens out after processing. I don't think the film is faulty, just marked
wrong. Maybe test a few sheets first, then use as usual.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: gantz-at-bu.edu [mailto:gantz-at-bu.edu]
Sent: January 7, 2009 4:14 PM
To: maryflet-at-interchange.ubc.ca

Dear Listers,
Today we have encountered what appears to be defective Kodak SO-163
electron image film, 3 1/4 x 4 inch, catalog #8010100. Those of you who
use this film will know that when loading the film into cassettes, if the
notch in the film is at the upper right-hand corner, then the emulsion side
of the film is up. The suspected defective film has the emulsion side down
with the notch at the upper right.

In addition, normally these packets of film have a slight upward curl
(concave) with the emulsion side up and notch to the upper right. This
potentially defective film has a downward curl (convex) with the notch to
the upper right.

On the box of film which is a multipak of 250 sheets, the following
numbers are found on the label:

Emul No. 239 002 07
2010-08
00277396

We do not know how widespread this problem is but we recommend not to use
film of this lot number until more information from Kodak is available.

Mr. Donald Gantz
Research Histologist/Electron Microscopist
Dept. Physiology and Biophysics
Center for Advanced Biomedical Research
Boston University School of Medicine
700 Albany Street
Boston, MA 02118
email: gantz-at-bu.edu
phone: 617-638-4017


==============================Original Headers==============================
7, 25 -- From gantz-at-bu.edu Wed Jan 7 18:10:14 2009
7, 25 -- Received: from relay15.bu.edu (relay15.bu.edu [128.197.27.66])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n080AECu008276
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 18:10:14
-0600
7, 25 -- X-Envelope-From: gantz-at-bu.edu
7, 25 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu
[155.41.208.134])
7, 25 -- by relay15.bu.edu (8.13.1/8.13.1) with ESMTP id
n0809mVA013635;
7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500
7, 25 -- Received: from gantz (cabr3-dhcp-208-153.bumc.bu.edu
[155.41.208.153])
7, 25 -- by biophysics1.bumc.bu.edu (8.13.8/8.13.8) with SMTP id
n0809mF4015021;
7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500
7, 25 -- Message-ID: {000b01c97125$69b0c190$99d0299b-at-gantz}
7, 25 -- From: "Don Gantz" {gantz-at-bu.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- Cc: {bullitt-at-bu.edu}
7, 25 -- Subject: Defective Electron Image Film
7, 25 -- Date: Wed, 7 Jan 2009 19:09:45 -0500
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="iso-8859-1"
7, 25 -- Content-Transfer-Encoding: 7bit
7, 25 -- X-Priority: 3
7, 25 -- X-MSMail-Priority: Normal
7, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807
7, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807
==============================End of - Headers==============================


==============================Original Headers==============================
16, 32 -- From maryflet-at-interchange.ubc.ca Thu Jan 8 12:41:25 2009
16, 32 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca [137.82.45.7])
16, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08IfOq3010949
16, 32 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 12:41:25 -0600
16, 32 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70])
16, 32 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id E6A591B276
16, 32 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 10:41:23 -0800 (PST)
16, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
16, 32 -- by smtp.interchange.ubc.ca
16, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
16, 32 -- with ESMTP id {0KD60098018Y29-at-smtp.interchange.ubc.ca} for
16, 32 -- microscopy-at-microscopy.com; Thu, 08 Jan 2009 10:41:23 -0800 (PST)
16, 32 -- Date: Thu, 08 Jan 2009 10:40:41 -0800
16, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
16, 32 -- Subject: RE: [Microscopy] Defective Electron Image Film
16, 32 -- In-reply-to: {200901080013.n080DvWV017600-at-ns.microscopy.com}
16, 32 -- To: gantz-at-bu.edu
16, 32 -- Cc: microscopy-at-microscopy.com
16, 32 -- Reply-to: maryflet-at-interchange.ubc.ca
16, 32 -- Message-id: {0KD60098118Y29-at-smtp.interchange.ubc.ca}
16, 32 -- Organization: Materials Eng.
16, 32 -- MIME-version: 1.0
16, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
16, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
16, 32 -- Content-type: text/plain; charset=us-ascii
16, 32 -- Content-transfer-encoding: 7bit
16, 32 -- Thread-index: AclxJgCge6cB0wedSUSS/HPlI4ibvQAlN+xg
16, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.1.8.182828
16, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
16, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, ECARD_WORD 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0
16, 32 -- X-Spam-Level:
16, 32 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: dhitrys-at-qimaging.com
Date: Thu, 8 Jan 2009 16:44:06 -0600
Subject: [Microscopy] PC or Mac preference for Digital Microscopy (4-question survey)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are doing a very quick survey about preferences for computer platforms
used for digital imaging microscopy.  There are only 4 questions (none of
them nasty multi-part!) so will take less than a minute to click on your
choices. 

Your input will help us provide the camera systems you need to take your
work to the next level, so thank you!!

http://www.zoomerang.com/Survey/?p=WEB228P5UWUTCB


--David Hitrys



==============================Original Headers==============================
7, 25 -- From dhitrys-at-qimaging.com Thu Jan 8 16:44:06 2009
7, 25 -- Received: from smtp02.lnh.mail.rcn.net (smtp02.lnh.mail.rcn.net [207.172.157.102])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08Mi6XH008842
7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 16:44:06 -0600
7, 25 -- Received: from mr08.lnh.mail.rcn.net ([207.172.157.28])
7, 25 -- by smtp02.lnh.mail.rcn.net with ESMTP; 08 Jan 2009 17:44:06 -0500
7, 25 -- Received: from smtp01.lnh.mail.rcn.net (smtp01.lnh.mail.rcn.net [207.172.4.11])
7, 25 -- by mr08.lnh.mail.rcn.net (MOS 3.10.4-GA)
7, 25 -- with ESMTP id KOD15676;
7, 25 -- Thu, 8 Jan 2009 17:43:44 -0500 (EST)
7, 25 -- Received: from 209-6-255-165.c3-0.frm-ubr2.sbo-frm.ma.cable.rcn.com (HELO DavidM1330) ([209.6.255.165])
7, 25 -- by smtp01.lnh.mail.rcn.net with ESMTP; 08 Jan 2009 17:43:44 -0500
7, 25 -- From: "David Hitrys" {dhitrys-at-qimaging.com}
7, 25 -- To: {microscopy-at-microscopy.com}
7, 25 -- Subject: PC or Mac preference for Digital Microscopy (4-question survey)
7, 25 -- Date: Thu, 8 Jan 2009 17:43:29 -0500
7, 25 -- Message-ID: {013401c971e2$872f6e70$958e4b50$-at-com}
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="iso-8859-1"
7, 25 -- X-Mailer: Microsoft Office Outlook 12.0
7, 25 -- Thread-Index: Aclx4n3tSxv5TJkiSJePgnsd+7BkFA==
7, 25 -- Content-Language: en-us
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n08Mi6XH008842
==============================End of - Headers==============================




From: abesenyo-at-ibilabs.com
Date: Thu, 8 Jan 2009 18:19:52 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abesenyo-at-ibilabs.com
Name: Alex Besenyo PhD

Organization: ibilabs

Title-Subject: [Filtered] uranyl compounds are alpha emitters only

Question: Question:

Is it true that the stuff we use has been somehow
depleted, so that it isn't as radioactive as "real" uranyl
salts? Or is this yet another old wive's tale of EM?!

Reply:

When we manufacture these compounds we purchase the raw uranium in a
depleted state from the government. There is no chance for error
here. We do not use natural uranium.

This means that the enrichable uranium U-235 has been removed.
The then U-238 which only emitts alpha radiation is procesed.

The term "depleted" means that U-235 has been removed.

If even by the slightest chance that U235 were present then every
alarm would go off in our facility because Beta and Gamma radiation
is detected.

I hope this answers everybodies concerns.

Our products are sold exclusively through a distributor network and
all of them have been instructed on this information.

I only responded when I saw the original post and I had to respond
before it got out of control.

Sincerely
Alex Besenyo PhD


Login Host: 74.173.69.139
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009
17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n090Jp6S032218
17, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52 -0600
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]}
17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: alerch-at-mcw.edu
Date: Thu, 8 Jan 2009 18:20:35 -0600
Subject: [Microscopy] viaWWW: How to find companies that buy/salvage older equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both alerch-at-mcw.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: alerch-at-mcw.edu
Name: Alexandra Lerch-Gaggl

Organization: Medical College of Wisconsin

Title-Subject: [Filtered] How to find companies that buy/salvage
older equipment

Question: We have a Biorad MRC 600 confocal microscope, where we had
the galvanos replaced a while ago. Now it seems that the scanner has
the same problem again. Is there a way that I could find companies or
institutions that salvage such equipment?
Thank you.

Login Host: 141.106.187.194
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:20:34 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n090KWOw000634
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:20:33 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p0624080bc58c48c0b5be-at-[206.69.208.22]}
6, 11 -- Date: Thu, 8 Jan 2009 18:20:30 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: alerch-at-mcw.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: How to find companies that buy/salvage older equipment
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Fri, 9 Jan 2009 01:38:48 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Alex,

Did you have a chance to check the information from

http://atom.kaeri.re.kr/ton/nuc7.html

Let me construct the decay tree. I'm sure everybody in this list can
do it but, you keep insisting that "uranyl compounds are alpha
emitters only" so, I will take the time to do the job and post in to
the list.

Let's start with U-238 which is the starting element in your compound.

1) U-238 decays into Th-234 by Alpha decay

2) Th-234 decays into Pa-234 by Beta decay

3) Pa-234 decays into U-234 by Beta decay

4) U-234 decays into Th-230 by Alpha decay

5) Th-230 decays into Ra-226 by Alpha decay

6) Ra-226 decays into Rn-222 by Alpha decay

7) Rn-222 decays into Po-218 by Alpha decay

8) Po-218 decays into Pb-214 by Alpha decay

9) Pb-214 decays into Bi-214 by Beta decay

10) Bi-214 decays into Po-214 by Beta decay

11) Po-214 decays into Pb-210 by Alpha decay

12) Pb-210 decays into Bi-210 by Beta decay

13) Bi-210 decays into Po-210 by Beta decay

14) Po-210 decays into Pb-206 by Alpha decay

Pb-206 is STABLE so, it is the last element to be produced as a result
of U-238 radioactive decay.

I have constructed the above decay tree using the information from
http://atom.kaeri.re.kr/ton/nuc7.html
While constructing the above decay tree I have used the branch which
has the highest branch ratio (above 99% in each case).

I do not understand why you are trying to keep things "under control"?

By the way, I'm a Nuclear Engineer.

One does not even need to be nuclear engineer to understand this. Even
in high school science classes people learn about radioactive decay
series e.g. A decays into B and B decays into C...

Best,
Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote:
}
} Email: abesenyo-at-ibilabs.com
} Name: Alex Besenyo PhD
}
} Organization: ibilabs
}
} Title-Subject: [Filtered] uranyl compounds are alpha emitters only
}
} Question: Question:
}
} Is it true that the stuff we use has been somehow
} depleted, so that it isn't as radioactive as "real" uranyl
} salts? Or is this yet another old wive's tale of EM?!
}
} Reply:
}
} When we manufacture these compounds we purchase the raw uranium in a
} depleted state from the government. There is no chance for error
} here. We do not use natural uranium.
}
} This means that the enrichable uranium U-235 has been removed.
} The then U-238 which only emitts alpha radiation is procesed.
}
} The term "depleted" means that U-235 has been removed.
}
} If even by the slightest chance that U235 were present then every
} alarm would go off in our facility because Beta and Gamma radiation
} is detected.
}
} I hope this answers everybodies concerns.
}
} Our products are sold exclusively through a distributor network and
} all of them have been instructed on this information.
}
} I only responded when I saw the original post and I had to respond
} before it got out of control.
}
} Sincerely
} Alex Besenyo PhD
}
}
}

==============================Original Headers==============================
28, 37 -- From celikaktas-at-gmail.com Fri Jan 9 01:38:48 2009
28, 37 -- Received: from mail-bw0-f12.google.com (mail-bw0-f12.google.com [209.85.218.12])
28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n097cllZ007406
28, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 01:38:48 -0600
28, 37 -- Received: by bwz5 with SMTP id 5so20323150bwz.18
28, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08 Jan 2009 23:38:46 -0800 (PST)
28, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
28, 37 -- d=gmail.com; s=gamma;
28, 37 -- h=domainkey-signature:received:received:message-id:date:from:to
28, 37 -- :subject:in-reply-to:mime-version:content-type
28, 37 -- :content-transfer-encoding:content-disposition:references;
28, 37 -- bh=x3VBln30706ul3mo8rzkKk8tdVV0mnTtpGId5uaYeEM=;
28, 37 -- b=qBVYz0biX1/wFlDLibyks38NncsgFjqElzGSuPXOiX+ZCB902M5i3lsMmCryurouqZ
28, 37 -- q2vvqo4qq+LQCiFwGfBQltrqfo1jiWYiWnPMwvN3V4b9MDoZd/yjgF76rgbzKDtibrjg
28, 37 -- Ob/1zXIpi/2mHaPBWSu4mzAG29Fqx67ZL1YFg=
28, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
28, 37 -- d=gmail.com; s=gamma;
28, 37 -- h=message-id:date:from:to:subject:in-reply-to:mime-version
28, 37 -- :content-type:content-transfer-encoding:content-disposition
28, 37 -- :references;
28, 37 -- b=DNSaPkpBjjjni4T+G+pPUuxo3M1nntLysfMKUnfOWQUEElcsWGJ3PmM4uVFQBbtTpT
28, 37 -- g+IO1lpZUOd3KJn43j3ed/pgCrIp1p0oTRjteC1sfQN2njKF2xkzfFZtTt2rNMbo4jBE
28, 37 -- /h849iT1rgdv5cvNLmKbW91LhMbKuR486Rzbg=
28, 37 -- Received: by 10.103.217.7 with SMTP id u7mr1380019muq.125.1231486725631;
28, 37 -- Thu, 08 Jan 2009 23:38:45 -0800 (PST)
28, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan 2009 23:38:45 -0800 (PST)
28, 37 -- Message-ID: {1075c5c10901082338y14d2b189m26ca5f198f5a9454-at-mail.gmail.com}
28, 37 -- Date: Fri, 9 Jan 2009 09:38:45 +0200
28, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com}
28, 37 -- To: abesenyo-at-ibilabs.com, microscopy {Microscopy-at-microscopy.com}
28, 37 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters only
28, 37 -- In-Reply-To: {200901090027.n090RYH1022494-at-ns.microscopy.com}
28, 37 -- MIME-Version: 1.0
28, 37 -- Content-Type: text/plain; charset=UTF-8
28, 37 -- Content-Transfer-Encoding: 7bit
28, 37 -- Content-Disposition: inline
28, 37 -- References: {200901090027.n090RYH1022494-at-ns.microscopy.com}
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 9 Jan 2009 08:36:22 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ayten,

Your comments are informative but, to my personal taste, definitely a
bit negative in tone for this list. Your "schooling" of Alex isn't
really necessary and I think we could get your information in a more
positive and collegial way.

Regarding the decay tree, I note from the provided link information that
the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
my high school chemistry but I'm wondering how much Th-234 and
associated beta emission danger we are really dealing with here; seems
like there must be a very small amount of Th-234 produced with such a
long half-life of the original U-238. Maybe you could comment on the
danger of this.

Thanks,

Dale


celikaktas-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Alex,
}
} Did you have a chance to check the information from
}
} http://atom.kaeri.re.kr/ton/nuc7.html
}
} Let me construct the decay tree. I'm sure everybody in this list can
} do it but, you keep insisting that "uranyl compounds are alpha
} emitters only" so, I will take the time to do the job and post in to
} the list.
}
} Let's start with U-238 which is the starting element in your compound.
}
} 1) U-238 decays into Th-234 by Alpha decay
}
} 2) Th-234 decays into Pa-234 by Beta decay
}
} 3) Pa-234 decays into U-234 by Beta decay
}
} 4) U-234 decays into Th-230 by Alpha decay
}
} 5) Th-230 decays into Ra-226 by Alpha decay
}
} 6) Ra-226 decays into Rn-222 by Alpha decay
}
} 7) Rn-222 decays into Po-218 by Alpha decay
}
} 8) Po-218 decays into Pb-214 by Alpha decay
}
} 9) Pb-214 decays into Bi-214 by Beta decay
}
} 10) Bi-214 decays into Po-214 by Beta decay
}
} 11) Po-214 decays into Pb-210 by Alpha decay
}
} 12) Pb-210 decays into Bi-210 by Beta decay
}
} 13) Bi-210 decays into Po-210 by Beta decay
}
} 14) Po-210 decays into Pb-206 by Alpha decay
}
} Pb-206 is STABLE so, it is the last element to be produced as a result
} of U-238 radioactive decay.
}
} I have constructed the above decay tree using the information from
} http://atom.kaeri.re.kr/ton/nuc7.html
} While constructing the above decay tree I have used the branch which
} has the highest branch ratio (above 99% in each case).
}
} I do not understand why you are trying to keep things "under control"?
}
} By the way, I'm a Nuclear Engineer.
}
} One does not even need to be nuclear engineer to understand this. Even
} in high school science classes people learn about radioactive decay
} series e.g. A decays into B and B decays into C...
}
} Best,
} Ayten.
}

==============================Original Headers==============================
7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009
7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09EaMPb032435
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 08:36:22 -0600
7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
7, 23 -- (authenticated bits=0)
7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n09EaLpI010451
7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 09:36:22 -0500
7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu}
7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500
7, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
7, 23 -- Reply-To: dac-at-research.umass.edu
7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
7, 23 -- MIME-Version: 1.0
7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
7, 23 -- only
7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 23 -- Content-Transfer-Encoding: 7bit
7, 23 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: jinsong-wu-at-northwestern.edu
Date: Fri, 9 Jan 2009 09:37:02 -0600
Subject: [Microscopy] viaWWW: cryo-TEM positions at Northwestern Univ

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jinsong-wu-at-northwestern.edu as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jinsong-wu-at-northwestern.edu
Name: jinsong wu

Organization: northwestern university

Title-Subject: [Filtered] cryo-TEM positions at Northwestern Univ.

Question: Two Open Positions
Research Associate or Research Faculty
Cryo-Electron Microscopy of Soft and Biological Structures
Northwestern University, Evanston, IL
Two research associate/research faculty positions are immediately available at
Northwestern University in the area of analytical
cryo- electron microscopy of soft and
biological structures.
Supported by the Keck Foundation, Chicago Biomedical Consortium and other
federally funded research programs, the two positions are created to advance
specimen preparation of biological structures for
electron microscopy, especially
oocytes, and the use of analytical TEM/STEM techniques to monitor bioelemental
distribution across sub-cellular compartments at nanoscale spatial resolution.
The research will principally employ a unique dual-EDS dedicated Hitachi cryo-
STEM (based on HD-2300A platform) to be installed
at Northwestern in summer 2009.
In addition, the Northwestern University Atomic and Nanoscale Characterization
Experimental (NUANCE) Center (www.nuance.northwestern.edu) and Quantitative
Bioelement Imaging Center (QBIC) in the in the
Chemistry of Life Processes Institute
(http://www.clp.northwestern.edu/) have several SEMs/S/TEMs and complementary
confocal, optical, scanning probe and
laser-ablation mass spectrometry capabilities.
The candidates are expected to develop cryo-specimen preparation protocols for
oocytes and conduct analytical studies of
elemental distribution in complex biological
structures using STEM imaging and EDS analysis.
The project is a part of extensive
interdisciplinary collaborative initiatives among
Professors Vinayak P. Dravid (www.northwestern.edu/vpdgroup), Tom OíHalloran
(http://chemgroups.northwestern.edu/ohalloran/), Teresa Woodruff
(http://www.northwestern.edu/neurobiology/faculty/Woodruff2/) and Jonathan
Silverstein (http://home.uchicago.edu/~jcs/) to
unravel the mysteries of nanoscale
chemical architecture of Oocyte at various stages
of fertilization. As a result, there are
ample opportunities for personal and professional
growth at the intersection of life and
physical sciences, medicine and advanced instrumentation.
The positions require a PhD in physical/biological sciences/engineering.
Considerable experience in analytical and cryo-S/TEM is required and hands-on
training in cryo-preparation techniques is highly
desirable. Prior knowledge of imaging
filter/spectral imaging and EELS is desirable but
not mandatory. The positions are
available immediately for at least two years,
with the possibility for extension for
additional period upon mutual agreement.
Salary and compensation would commensurate with experience.
Please forward curriculum vitae with three references to: Mr. Kim McCumber
Email: ohalloran-ofc-at-northwestern.edu

Login Host: 129.105.37.144
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Fri Jan 9 09:37:02 2009
7, 13 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09Fb0XB015374
7, 13 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 09:37:01 -0600
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240800c58d1f8a0e09-at-[206.69.208.22]}
7, 13 -- Date: Fri, 9 Jan 2009 09:36:59 -0600
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: jinsong-wu-at-northwestern.edu (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: cryo-TEM positions at Northwestern Univ
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09Fb0XB015374
==============================End of - Headers==============================




From: cervantes-at-bendres.com
Date: Fri, 9 Jan 2009 12:37:17 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I work in a place that does not permit the use of Uranyl salts for EM
use because of the radiation dangers (and a healthy respect for ALARA),
and am always trying to educate people about what these dangers are with
facts. Thus I was very interested in this thread.

I did have a question on the post by Ayten that I hope someone will
answer: I am not a nuclear engineer, so I readily acknowledge that I am
far from an expert on the subject. But the website Ayten provided gives
the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with
the half-life given in Dale's response). Would that not also make the
amount of beta radiation small?

It was interesting to read in Alex's post about how the presence of
U-235 causes alarms to go off. Commercially available (United States,
Electron Microscopy Sciences, technical data sheet for Uranyl Acetate,
available at www.emsdiasum.com) is listed as 0.1% U235, so there is
*some* U235 present, at least for this supplier (Ted Pella lists the
composition as 0.3-0.4% U235). EMS also gives a reading for Alpha and
Beta radiation for a 100 g sample, and gives a value of .51 uCi/g for
the specific activity (they state a material with a value of } 0.002
uCi/g is considered radioactive).

I would also like to agree with Dale on his remarks on the tone of
Ayten's response. This should be a place that, even though we may
disagree, our mutual respect for one another allows us to be civil and
polite, and fosters healthy debates in cases of contention.

Thank you,
Jessica


____________________
Jessica Cervantes, MS



-----Original Message-----
X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu]
Sent: Friday, January 09, 2009 6:42 AM
To: Cervantes, Jessica

Dear Ayten,

Your comments are informative but, to my personal taste, definitely a
bit negative in tone for this list. Your "schooling" of Alex isn't
really necessary and I think we could get your information in a more
positive and collegial way.

Regarding the decay tree, I note from the provided link information that

the half-life of U-238 is { {4.468E+9 years} } . It has been a while since

my high school chemistry but I'm wondering how much Th-234 and
associated beta emission danger we are really dealing with here; seems
like there must be a very small amount of Th-234 produced with such a
long half-life of the original U-238. Maybe you could comment on the
danger of this.

Thanks,

Dale


celikaktas-at-gmail.com wrote:
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Dear Alex,
}
} Did you have a chance to check the information from
}
} http://atom.kaeri.re.kr/ton/nuc7.html
}
} Let me construct the decay tree. I'm sure everybody in this list can
} do it but, you keep insisting that "uranyl compounds are alpha
} emitters only" so, I will take the time to do the job and post in to
} the list.
}
} Let's start with U-238 which is the starting element in your compound.
}
} 1) U-238 decays into Th-234 by Alpha decay
}
} 2) Th-234 decays into Pa-234 by Beta decay
}
} 3) Pa-234 decays into U-234 by Beta decay
}
} 4) U-234 decays into Th-230 by Alpha decay
}
} 5) Th-230 decays into Ra-226 by Alpha decay
}
} 6) Ra-226 decays into Rn-222 by Alpha decay
}
} 7) Rn-222 decays into Po-218 by Alpha decay
}
} 8) Po-218 decays into Pb-214 by Alpha decay
}
} 9) Pb-214 decays into Bi-214 by Beta decay
}
} 10) Bi-214 decays into Po-214 by Beta decay
}
} 11) Po-214 decays into Pb-210 by Alpha decay
}
} 12) Pb-210 decays into Bi-210 by Beta decay
}
} 13) Bi-210 decays into Po-210 by Beta decay
}
} 14) Po-210 decays into Pb-206 by Alpha decay
}
} Pb-206 is STABLE so, it is the last element to be produced as a result
} of U-238 radioactive decay.
}
} I have constructed the above decay tree using the information from
} http://atom.kaeri.re.kr/ton/nuc7.html
} While constructing the above decay tree I have used the branch which
} has the highest branch ratio (above 99% in each case).
}
} I do not understand why you are trying to keep things "under control"?
}
} By the way, I'm a Nuclear Engineer.
}
} One does not even need to be nuclear engineer to understand this. Even
} in high school science classes people learn about radioactive decay
} series e.g. A decays into B and B decays into C...
}
} Best,
} Ayten.
}

==============================Original
Headers==============================
7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009
7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu
[128.119.101.41])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n09EaMPb032435
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009
08:36:22 -0600
7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu
[128.119.55.30])
7, 23 -- (authenticated bits=0)
7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id
n09EaLpI010451
7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NOT)
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009
09:36:22 -0500
7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu}
7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500
7, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
7, 23 -- Reply-To: dac-at-research.umass.edu
7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US;
rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
7, 23 -- MIME-Version: 1.0
7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are
alpha emitters
7, 23 -- only
7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 23 -- Content-Transfer-Encoding: 7bit
7, 23 -- X-Whitelist: TRUE
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 22 -- From cervantes-at-bendres.com Fri Jan 9 12:37:17 2009
24, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113])
24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09IbGAs002132
24, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 12:37:17 -0600
24, 22 -- Content-class: urn:content-classes:message
24, 22 -- MIME-Version: 1.0
24, 22 -- Content-Type: text/plain;
24, 22 -- charset="us-ascii"
24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 22 -- Subject: RE: [Microscopy] viaWWW: uranyl compounds are alpha emitters
24, 22 -- Date: Fri, 9 Jan 2009 10:37:16 -0800
24, 22 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A040C1F-at-dixie.bri.local}
24, 22 -- In-Reply-To: {200901091442.n09Eg4GH008217-at-ns.microscopy.com}
24, 22 -- X-MS-Has-Attach:
24, 22 -- X-MS-TNEF-Correlator:
24, 22 -- Thread-Topic: [Microscopy] viaWWW: uranyl compounds are alpha emitters
24, 22 -- Thread-Index: AclyaHEzMn7sIVgATIeELTj+MUIUqwAFW70Q
24, 22 -- References: {200901091442.n09Eg4GH008217-at-ns.microscopy.com}
24, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com}
24, 22 -- To: {Microscopy-at-microscopy.com}
24, 22 -- Content-Transfer-Encoding: 8bit
24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09IbGAs002132
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 9 Jan 2009 13:38:14 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 9, 2009, at 10:37 AM, cervantes-at-bendres.com wrote:

} I did have a question on the post by Ayten that I hope someone will
} answer: I am not a nuclear engineer, so I readily acknowledge that I
} am
} far from an expert on the subject. But the website Ayten provided
} gives
} the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with
} the half-life given in Dale's response). Would that not also make the
} amount of beta radiation small?


Dear Jessica,
The amount of Th234 is, indeed, small, but the branching ratio for
U238 -} Th234 is close to 100%--the Handbook of Chemistry and Physics
also lists SF (spontaneous fission), but this is very rare. Assuming
that the process of depleting the U will also get rid of any Th
originally mixed in with the natural U, the long half life of U238
means that the amount of Th234 will be small for the first few billion
years or so. In any case, the ranges of both alpha and beta particles
in glass are smaller than the thickness of the bottle, so a jar of UA
will not have either alpha or beta particles traveling to the outside
world. The Handbook does also list 3 particle energies for U238 alpha
particles, which arise due to the possibility of decay into 3 states
of Th234--the ground state and two excited states. These excited
states will decay to the ground state, usually by emitting a gamma
ray, which will penetrate the jar, a good distance through the air,
and your hand (if you are holding the jar). It is these gammas that
pose the greatest risk from a sealed jar of UA. For the nit-pickers,
the gammas can scatter off atoms in the jar to produce secondary
electrons, some of which may be produced close enough to the outer
surface of the jar to penetrate into the air, so it is not entirely
accurate to say no beta radiation could ever be detected outside the
jar. As previously stated by several listers, the major danger from
UA is ingestion or inhalation. The same properties of alpha radiation
that prevent it from penetrating the dead layer of the skin also
dictate that the entire energy of the alpha decay will be deposited in
a small volume either on the inner surface of the lung or in the
digestive tract and other parts of the body that the U can be
transported to or deposited in. This large amount of energy will
produce many ion pairs, since one ion pair is produced for every ~30
eV of energy deposited, and the decay energy is 4.268 MeV. The damage
done to a cell in which these ions are produced is usually fatal to
the cell, but can also be severe without killing the cell, in which
case, the cell can become cancerous. Therefore, such activities as
weighing UA to make solutions, pipetting UA, which can produce very
small droplets that evaporate leaving a small particle of UA, and
other handling of UA, especially the solid from the jar, should always
be performed with the knowledge that there is risk. ALARA dictates
that these procedures be performed in a hood (into which there is a
substantial flow of air), and that they are only performed in a
limited area, which should be frequently monitored for spilled UA (and
any other radioactive materials that might be used) by measuring with
a detector and by taking swabs in and around the area that will also
be tested for the presence of radiation. Different states and
countries have different laws that determine the minimum amount of
monitoring, and different institutions have more restrictive policies,
so there is no single procedure that is universally followed, but it
is very important that each person follow the procedures EHS sets up
in one's lab, and even more important that everyone takes safety
seriously.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Fri Jan 9 13:38:14 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JcDnK017471
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:38:13 -0600
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 592CC32A786
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:38:13 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id E2E4A32A703
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:38:11 -0800 (PST)
6, 22 -- Message-Id: {B018E32F-06FA-4F4B-BE9C-6D4B5EB5C94E-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200901091837.n09IbP7p002260-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] RE: viaWWW: uranyl compounds are alpha emitters
6, 22 -- Date: Fri, 9 Jan 2009 11:38:11 -0800
6, 22 -- References: {200901091837.n09IbP7p002260-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Fri, 9 Jan 2009 13:44:24 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jessica:

I used to work as a 'radiochemist' making radioactive isotope products and
standards, including Uranium. FYI, you can calculate the specific activity,
A, from the data you cited:

A = yN, (y should be a 'lambda', sorry!), and A is disintigrations per time
(usually sec or min)

y = ln2 / T, where T is half life of isotope. (Convert half-life to seconds
to give disintegrations per second.)

N = number of atoms of that isotope (per gram for specific activity).

There are daughter products from the decay, and as you mentioned, there
might be U-235 as well, though the manufacturer stated it was clean of this.


By the way, after working as a radio / nuclear chemist, I don't subscribe
totally to the ALARA principle. We take about 360mrem/yr background just
living in USA. Also, a long roundtrip plane flight will give you up to
10mrem. The risks are negligible.

Hope that helps.

Don

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.


Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com


-----Original Message-----
X-from: cervantes-at-bendres.com [mailto:cervantes-at-bendres.com]
Sent: Friday, January 09, 2009 10:47 AM
To: dkloos-at-parallaxray.com

I work in a place that does not permit the use of Uranyl salts for EM
use because of the radiation dangers (and a healthy respect for ALARA),
and am always trying to educate people about what these dangers are with
facts. Thus I was very interested in this thread.

I did have a question on the post by Ayten that I hope someone will
answer: I am not a nuclear engineer, so I readily acknowledge that I am
far from an expert on the subject. But the website Ayten provided gives
the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with
the half-life given in Dale's response). Would that not also make the
amount of beta radiation small?

It was interesting to read in Alex's post about how the presence of
U-235 causes alarms to go off. Commercially available (United States,
Electron Microscopy Sciences, technical data sheet for Uranyl Acetate,
available at www.emsdiasum.com) is listed as 0.1% U235, so there is
*some* U235 present, at least for this supplier (Ted Pella lists the
composition as 0.3-0.4% U235). EMS also gives a reading for Alpha and
Beta radiation for a 100 g sample, and gives a value of .51 uCi/g for
the specific activity (they state a material with a value of } 0.002
uCi/g is considered radioactive).

I would also like to agree with Dale on his remarks on the tone of
Ayten's response. This should be a place that, even though we may
disagree, our mutual respect for one another allows us to be civil and
polite, and fosters healthy debates in cases of contention.

Thank you,
Jessica


____________________
Jessica Cervantes, MS



-----Original Message-----
X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu]
Sent: Friday, January 09, 2009 6:42 AM
To: Cervantes, Jessica

Dear Ayten,

Your comments are informative but, to my personal taste, definitely a
bit negative in tone for this list. Your "schooling" of Alex isn't
really necessary and I think we could get your information in a more
positive and collegial way.

Regarding the decay tree, I note from the provided link information that

the half-life of U-238 is { {4.468E+9 years} } . It has been a while since

my high school chemistry but I'm wondering how much Th-234 and
associated beta emission danger we are really dealing with here; seems
like there must be a very small amount of Th-234 produced with such a
long half-life of the original U-238. Maybe you could comment on the
danger of this.

Thanks,

Dale


celikaktas-at-gmail.com wrote:
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Dear Alex,
}
} Did you have a chance to check the information from
}
} http://atom.kaeri.re.kr/ton/nuc7.html
}
} Let me construct the decay tree. I'm sure everybody in this list can
} do it but, you keep insisting that "uranyl compounds are alpha
} emitters only" so, I will take the time to do the job and post in to
} the list.
}
} Let's start with U-238 which is the starting element in your compound.
}
} 1) U-238 decays into Th-234 by Alpha decay
}
} 2) Th-234 decays into Pa-234 by Beta decay
}
} 3) Pa-234 decays into U-234 by Beta decay
}
} 4) U-234 decays into Th-230 by Alpha decay
}
} 5) Th-230 decays into Ra-226 by Alpha decay
}
} 6) Ra-226 decays into Rn-222 by Alpha decay
}
} 7) Rn-222 decays into Po-218 by Alpha decay
}
} 8) Po-218 decays into Pb-214 by Alpha decay
}
} 9) Pb-214 decays into Bi-214 by Beta decay
}
} 10) Bi-214 decays into Po-214 by Beta decay
}
} 11) Po-214 decays into Pb-210 by Alpha decay
}
} 12) Pb-210 decays into Bi-210 by Beta decay
}
} 13) Bi-210 decays into Po-210 by Beta decay
}
} 14) Po-210 decays into Pb-206 by Alpha decay
}
} Pb-206 is STABLE so, it is the last element to be produced as a result
} of U-238 radioactive decay.
}
} I have constructed the above decay tree using the information from
} http://atom.kaeri.re.kr/ton/nuc7.html
} While constructing the above decay tree I have used the branch which
} has the highest branch ratio (above 99% in each case).
}
} I do not understand why you are trying to keep things "under control"?
}
} By the way, I'm a Nuclear Engineer.
}
} One does not even need to be nuclear engineer to understand this. Even
} in high school science classes people learn about radioactive decay
} series e.g. A decays into B and B decays into C...
}
} Best,
} Ayten.
}

==============================Original
Headers==============================
7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009
7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu
[128.119.101.41])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n09EaMPb032435
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009
08:36:22 -0600
7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu
[128.119.55.30])
7, 23 -- (authenticated bits=0)
7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id
n09EaLpI010451
7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NOT)
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009
09:36:22 -0500
7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu}
7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500
7, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
7, 23 -- Reply-To: dac-at-research.umass.edu
7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US;
rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
7, 23 -- MIME-Version: 1.0
7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are
alpha emitters
7, 23 -- only
7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com}
7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 23 -- Content-Transfer-Encoding: 7bit
7, 23 -- X-Whitelist: TRUE
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 22 -- From cervantes-at-bendres.com Fri Jan 9 12:37:17 2009
24, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113])
24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n09IbGAs002132
24, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 12:37:17
-0600
24, 22 -- Content-class: urn:content-classes:message
24, 22 -- MIME-Version: 1.0
24, 22 -- Content-Type: text/plain;
24, 22 -- charset="us-ascii"
24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 22 -- Subject: RE: [Microscopy] viaWWW: uranyl compounds are alpha
emitters
24, 22 -- Date: Fri, 9 Jan 2009 10:37:16 -0800
24, 22 -- Message-ID:
{82C755170EE5C44BA26E35A7F6B3864A040C1F-at-dixie.bri.local}
24, 22 -- In-Reply-To: {200901091442.n09Eg4GH008217-at-ns.microscopy.com}
24, 22 -- X-MS-Has-Attach:
24, 22 -- X-MS-TNEF-Correlator:
24, 22 -- Thread-Topic: [Microscopy] viaWWW: uranyl compounds are alpha
emitters
24, 22 -- Thread-Index: AclyaHEzMn7sIVgATIeELTj+MUIUqwAFW70Q
24, 22 -- References: {200901091442.n09Eg4GH008217-at-ns.microscopy.com}
24, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com}
24, 22 -- To: {Microscopy-at-microscopy.com}
24, 22 -- Content-Transfer-Encoding: 8bit
24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n09IbGAs002132
==============================End of - Headers==============================


==============================Original Headers==============================
41, 30 -- From dkloos-at-parallaxray.com Fri Jan 9 13:44:23 2009
41, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
41, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JiLtE026720
41, 30 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:44:22 -0600
41, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
41, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
41, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
41, 30 -- id 1LLNHN-000AxF-NI; Fri, 09 Jan 2009 12:44:25 -0700
41, 30 -- Reply-To: {dkloos-at-parallaxray.com}
41, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
41, 30 -- To: {cervantes-at-bendres.com}
41, 30 -- Cc: {microscopy-at-microscopy.com}
41, 30 -- References: {200901091847.n09IlB3t015536-at-ns.microscopy.com}
41, 30 -- Subject: RE: [Microscopy] RE: viaWWW: uranyl compounds are alpha emitters
41, 30 -- Date: Fri, 9 Jan 2009 11:44:13 -0800
41, 30 -- Organization: Parallax Research
41, 30 -- Message-ID: {E956386CB52E4F8488CB5A61C09C99D8-at-donl}
41, 30 -- MIME-Version: 1.0
41, 30 -- Content-Type: text/plain;
41, 30 -- charset="us-ascii"
41, 30 -- Content-Transfer-Encoding: 7bit
41, 30 -- X-Mailer: Microsoft Office Outlook 11
41, 30 -- Thread-Index: AclyirXsDrkzft58SYy5CgCv2E93aQABANEw
41, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
41, 30 -- In-Reply-To: {200901091847.n09IlB3t015536-at-ns.microscopy.com}
41, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
41, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
41, 30 -- X-AntiAbuse: Original Domain - microscopy.com
41, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
41, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 9 Jan 2009 13:56:58 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 9, 2009, at 11:44 AM, dkloos-at-parallaxray.com wrote:

} By the way, after working as a radio / nuclear chemist, I don't
} subscribe
} totally to the ALARA principle. We take about 360mrem/yr background
} just
} living in USA. Also, a long roundtrip plane flight will give you up
} to
} 10mrem. The risks are negligible.


Dear Don,
It is very true that there is background radiation, and it is still
not known whether any increases over background cause a proportional
increase in risk, but since the R stands for "reasonably", ALARA
should be followed.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Fri Jan 9 13:56:58 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JuwMj012369
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:56:58 -0600
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id DBC3C328E87
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:56:57 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id DA21532A846
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:56:56 -0800 (PST)
6, 22 -- Message-Id: {D4A7F8CA-339B-441B-ADF1-3F617A2A4FD4-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200901091944.n09JiUPN026950-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters
6, 22 -- Date: Fri, 9 Jan 2009 11:56:56 -0800
6, 22 -- References: {200901091944.n09JiUPN026950-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: kamlennon-at-yahoo.com
Date: Fri, 9 Jan 2009 13:57:15 -0600
Subject: [Microscopy] Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Again Listers,

Thanks to all of you who have responded to my recent query. I'm sure that I will have more as time passes, and I appreciate the fantastic response that I've had from the list.

Since we are on the topic of the dangers of EM work (the uranyl acetate thread), I have a question about cacodylate buffer. I'm getting set to teach an introductory EM course for biologists to undergraduates. Having interviewed two of my three students during the previous semester, I know that I will be starting very much at zero. They had no to little knowledge of what "EM" is or can be used for before I spoke with them - they are exploring this new class. My plan is to take them through the preparation of plant, animal, and some sort of micro sample via traditional chemical fixation methods and keep it as simple as possible. I am inclined to steer clear of cacodylate buffer due to its toxicity and because they have enough to deal with already, and stick with phosphate buffer. However, I have noticed that most if not all of the animal tissue protocols I've been perusing use cac buffer. Is there any reason why I should keep it in the protocol?

Thanks for your advice.
Kristen

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
202 Compton Science Center
Frostburg State University
101 Braddock Road
Frostburg, MD 21532

k.lennon-at-frostburg.edu




==============================Original Headers==============================
7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009
7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09JvEx0012745
7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 13:57:14 -0600
7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9 Jan 2009 19:57:14 -0000
7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 20 -- s=s1024; d=yahoo.com;
7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
7, 20 -- b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=;
7, 20 -- X-YMail-OSG: LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg--
7, 20 -- Received: from [96.239.149.81] by web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009 11:57:13 PST
7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST)
7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
7, 20 -- Reply-To: kamlennon-at-yahoo.com
7, 20 -- Subject: Cac buffer and undergrads - chancy?
7, 20 -- To: Microscopy-at-Microscopy.Com
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain; charset=us-ascii
7, 20 -- Message-ID: {982974.76126.qm-at-web84004.mail.mud.yahoo.com}
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 9 Jan 2009 14:13:13 -0600
Subject: [Microscopy] Re: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kristen,

There are 2 ways of looking at this: first, phosphate and other
buffers work as well as cacodylate, the difference between cac and
PO4, e.g., is mostly preference and what one is used to seeing. So
why use a toxin that can be avoided? Especially since some people
(like me) are sensitive to the arsenic.
The other way is: sometimes cacodylate is needed, and the students
will have to be using other toxic materials in microscopy and
chemistry and biotechnology and ... in other words, they need to
learn how to properly handle such materials, and the sooner they
learn the better, so use it.

There, microambiguity. In our EM courses, I avoid cacodylate (because
I'm sensitive), but we do make it available if needed, or if a
student wants to use it for a project.

Generally, though, when you're teaching about buffers, there are a
lot more to discuss than just PO4 or cacodylate. If the class is
doing aquatic critters (algae, protistans, tiny inverts, and
suchlike), then the best buffer is 0.02 micron filtered water from
where the crittere were collected.

Phil

} Hello Again Listers,
}
} Thanks to all of you who have responded to my recent query. I'm sure
} that I will have more as time passes, and I appreciate the fantastic
} response that I've had from the list.
}
} Since we are on the topic of the dangers of EM work (the uranyl
} acetate thread), I have a question about cacodylate buffer. I'm
} getting set to teach an introductory EM course for biologists to
} undergraduates. Having interviewed two of my three students during
} the previous semester, I know that I will be starting very much at
} zero. They had no to little knowledge of what "EM" is or can be used
} for before I spoke with them - they are exploring this new class. My
} plan is to take them through the preparation of plant, animal, and
} some sort of micro sample via traditional chemical fixation methods
} and keep it as simple as possible. I am inclined to steer clear of
} cacodylate buffer due to its toxicity and because they have enough
} to deal with already, and stick with phosphate buffer. However, I
} have noticed that most if not all of the animal tissue protocols
} I've been perusing use cac buffer. Is there any reason why I should
} keep it in the protocol?
}
} Thanks for your advice.
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} k.lennon-at-frostburg.edu
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
6, 25 -- From oshel1pe-at-cmich.edu Fri Jan 9 14:13:13 2009
6, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KDCn7007223
6, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:13:13 -0600
6, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n09KDBgb017674
6, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:13:11 -0500
6, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Fri, 9 Jan 2009 15:13:02 -0500
6, 25 -- Mime-Version: 1.0
6, 25 -- Message-Id: {f06240804c58d5e6fc0f6-at-[141.209.160.249]}
6, 25 -- In-Reply-To: {200901092000.n09K0PVw024065-at-ns.microscopy.com}
6, 25 -- References: {200901092000.n09K0PVw024065-at-ns.microscopy.com}
6, 25 -- Date: Fri, 9 Jan 2009 15:13:00 -0500
6, 25 -- To: Microscopy-at-microscopy.com
6, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 25 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
6, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:02.0896 (UTC) FILETIME=[ACF1D300:01C97296]
6, 25 -- X-Canit-CHI2: 0.00
6, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
6, 25 -- X-CanItPRO-Stream: default
6, 25 -- X-Canit-Stats-ID: 7251385 - 9ad74a6e6749
6, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 9 Jan 2009 14:13:53 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ayten,

As a nuclear engineer you should know better about dangers of radioactivity (by the way, by training I am a physicist.)

Decay tree says us nothing about these dangers. We should know real levels of radiation. What can you say about them? Is level of radiation at 5 inches from open bottle with 25 g of uranil acetate much greater then the one from granite countertop in someone kitchen? I do not know. Since I do not know, I will be cautious, but just cautious, not paranoid.

Let’s see what World Health Association says:
http://www.who.int/mediacentre/factsheets/fs257/en/

“DEPLETED URANIUM
• On average, approximately 90 µg (micrograms) of uranium exists in the human body from normal intakes of water, food and air. About 66% is found in the skeleton, 16% in the liver, 8% in the kidneys and 10% in other tissues. {90 micrograms of uranium, not depleted uranium}
• The uranium remaining after removal of the enriched fraction contains about 99.8% 238U, 0.2% 235U and 0.001% 234U by mass; this is referred to as depleted uranium or DU.
• Under most circumstances, use of DU will make a negligible contribution to the overall natural background levels of uranium in the environment. Probably the greatest potential for DU exposure will follow conflict where DU munitions are used.
• Average annual intakes of uranium by adults are estimated to be about 0.5mg (500 μg) from ingestion of food and water and 0.6 μg from breathing air.â€

So, uranium is everywhere. We should not bother ourselves with decay tree. If in doubt, we should measure radiation. And, of course, we should remember about toxicity of uranium. Gloves, fume hoods, proper treatment of spillage, etc. And when required, we should collect and dispose used chemicals in proper way.

I beg your pardon if you find my reply a bit harsh. I just followed your style.

Regards,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
} Sent: Friday, January 09, 2009 1:39 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha
} emitters only
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Dear Alex,
}
} Did you have a chance to check the information from
}
} http://atom.kaeri.re.kr/ton/nuc7.html
}
} Let me construct the decay tree. I'm sure everybody in this
} list can do it but, you keep insisting that "uranyl compounds
} are alpha emitters only" so, I will take the time to do the
} job and post in to the list.
}
} Let's start with U-238 which is the starting element in your compound.
}
} 1) U-238 decays into Th-234 by Alpha decay
}
} 2) Th-234 decays into Pa-234 by Beta decay
}
} 3) Pa-234 decays into U-234 by Beta decay
}
} 4) U-234 decays into Th-230 by Alpha decay
}
} 5) Th-230 decays into Ra-226 by Alpha decay
}
} 6) Ra-226 decays into Rn-222 by Alpha decay
}
} 7) Rn-222 decays into Po-218 by Alpha decay
}
} 8) Po-218 decays into Pb-214 by Alpha decay
}
} 9) Pb-214 decays into Bi-214 by Beta decay
}
} 10) Bi-214 decays into Po-214 by Beta decay
}
} 11) Po-214 decays into Pb-210 by Alpha decay
}
} 12) Pb-210 decays into Bi-210 by Beta decay
}
} 13) Bi-210 decays into Po-210 by Beta decay
}
} 14) Po-210 decays into Pb-206 by Alpha decay
}
} Pb-206 is STABLE so, it is the last element to be produced as
} a result of U-238 radioactive decay.
}
} I have constructed the above decay tree using the information
} from http://atom.kaeri.re.kr/ton/nuc7.html
} While constructing the above decay tree I have used the
} branch which has the highest branch ratio (above 99% in each case).
}
} I do not understand why you are trying to keep things "under control"?
}
} By the way, I'm a Nuclear Engineer.
}
} One does not even need to be nuclear engineer to understand
} this. Even in high school science classes people learn about
} radioactive decay series e.g. A decays into B and B decays into C...
}
} Best,
} Ayten.
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
}
} On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote:
} }
} } Email: abesenyo-at-ibilabs.com
} } Name: Alex Besenyo PhD
} }
} } Organization: ibilabs
} }
} } Title-Subject: [Filtered] uranyl compounds are alpha emitters only
} }
} } Question: Question:
} }
} } Is it true that the stuff we use has been somehow
} } depleted, so that it isn't as radioactive as "real" uranyl
} } salts? Or is this yet another old wive's tale of EM?!
} }
} } Reply:
} }
} } When we manufacture these compounds we purchase the raw uranium in a
} } depleted state from the government. There is no chance for error
} } here. We do not use natural uranium.
} }
} } This means that the enrichable uranium U-235 has been removed.
} } The then U-238 which only emitts alpha radiation is procesed.
} }
} } The term "depleted" means that U-235 has been removed.
} }
} } If even by the slightest chance that U235 were present then every
} } alarm would go off in our facility because Beta and Gamma radiation
} } is detected.
} }
} } I hope this answers everybodies concerns.
} }
} } Our products are sold exclusively through a distributor network and
} } all of them have been instructed on this information.
} }
} } I only responded when I saw the original post and I had to respond
} } before it got out of control.
} }
} } Sincerely
} } Alex Besenyo PhD
} }
} }
} }
}
} ==============================Original
} Headers==============================
} 28, 37 -- From celikaktas-at-gmail.com Fri Jan 9 01:38:48 2009
} 28, 37 -- Received: from mail-bw0-f12.google.com
} (mail-bw0-f12.google.com [209.85.218.12])
} 28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n097cllZ007406
} 28, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan
} 2009 01:38:48 -0600
} 28, 37 -- Received: by bwz5 with SMTP id 5so20323150bwz.18
} 28, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08
} Jan 2009 23:38:46 -0800 (PST)
} 28, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 28, 37 -- d=gmail.com; s=gamma;
} 28, 37 --
} h=domainkey-signature:received:received:message-id:date:from:to
} 28, 37 -- :subject:in-reply-to:mime-version:content-type
} 28, 37 --
} :content-transfer-encoding:content-disposition:references;
} 28, 37 -- bh=x3VBln30706ul3mo8rzkKk8tdVV0mnTtpGId5uaYeEM=;
} 28, 37 --
} b=qBVYz0biX1/wFlDLibyks38NncsgFjqElzGSuPXOiX+ZCB902M5i3lsMmCryurouqZ
} 28, 37 --
} q2vvqo4qq+LQCiFwGfBQltrqfo1jiWYiWnPMwvN3V4b9MDoZd/yjgF76rgbzKDtibrjg
} 28, 37 -- Ob/1zXIpi/2mHaPBWSu4mzAG29Fqx67ZL1YFg=
} 28, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 28, 37 -- d=gmail.com; s=gamma;
} 28, 37 --
} h=message-id:date:from:to:subject:in-reply-to:mime-version
} 28, 37 --
} :content-type:content-transfer-encoding:content-disposition
} 28, 37 -- :references;
} 28, 37 --
} b=DNSaPkpBjjjni4T+G+pPUuxo3M1nntLysfMKUnfOWQUEElcsWGJ3PmM4uVFQBbtTpT
} 28, 37 --
} g+IO1lpZUOd3KJn43j3ed/pgCrIp1p0oTRjteC1sfQN2njKF2xkzfFZtTt2rNMbo4jBE
} 28, 37 -- /h849iT1rgdv5cvNLmKbW91LhMbKuR486Rzbg=
} 28, 37 -- Received: by 10.103.217.7 with SMTP id
} u7mr1380019muq.125.1231486725631;
} 28, 37 -- Thu, 08 Jan 2009 23:38:45 -0800 (PST)
} 28, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan
} 2009 23:38:45 -0800 (PST)
} 28, 37 -- Message-ID:
} {1075c5c10901082338y14d2b189m26ca5f198f5a9454-at-mail.gmail.com}
} 28, 37 -- Date: Fri, 9 Jan 2009 09:38:45 +0200
} 28, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com}
} 28, 37 -- To: abesenyo-at-ibilabs.com, microscopy
} {Microscopy-at-microscopy.com}
} 28, 37 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds
} are alpha emitters only
} 28, 37 -- In-Reply-To: {200901090027.n090RYH1022494-at-ns.microscopy.com}
} 28, 37 -- MIME-Version: 1.0
} 28, 37 -- Content-Type: text/plain; charset=UTF-8
} 28, 37 -- Content-Transfer-Encoding: 7bit
} 28, 37 -- Content-Disposition: inline
} 28, 37 -- References: {200901090027.n090RYH1022494-at-ns.microscopy.com}
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
14, 25 -- From DusevichV-at-umkc.edu Fri Jan 9 14:13:53 2009
14, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KDquJ008557
14, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:13:52 -0600
14, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 25 -- Fri, 9 Jan 2009 14:13:44 -0600
14, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 25 -- Content-class: urn:content-classes:message
14, 25 -- MIME-Version: 1.0
14, 25 -- Content-Type: text/plain;
14, 25 -- charset="UTF-8"
14, 25 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only
14, 25 -- Date: Fri, 9 Jan 2009 14:13:44 -0600
14, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AA-at-KC-MSX1.kc.umkc.edu}
14, 25 -- In-Reply-To: {200901090739.n097dQZk008263-at-ns.microscopy.com}
14, 25 -- X-MS-Has-Attach:
14, 25 -- X-MS-TNEF-Correlator:
14, 25 -- Thread-Topic: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only
14, 25 -- thread-index: AclyLWbfA0iBUCGrQXeRBZzilk1+5QAaS2kQ
14, 25 -- References: {200901090739.n097dQZk008263-at-ns.microscopy.com}
14, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
14, 25 -- To: {Microscopy-at-microscopy.com}
14, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:44.0274 (UTC) FILETIME=[C59B9B20:01C97296]
14, 25 -- Content-Transfer-Encoding: 8bit
14, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n09KDquJ008557
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 9 Jan 2009 14:31:19 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I have found this conversation both enlightening and interesting. One
of the things I found interesting is to guess at the location of the
submitter. This gives me a measure of my bias. I will say that we have
flogged this horse to death, but I want to share a bit of my bias.

The total elimination of risk, no matter if you perceive it as
reasonable or not, seems to be a major pre-occupation of our society.
This seems to be driven by people whose livelihood is derived from
telling us about these dangers. One might expect a bias from them.
After all if they are not making you safer, they are not doing their
job.

It is difficult to argue against safety precautions and still seem
rational, it appears to me that we are taking safety to unreasonable
heights. We need to be reminded that nobody gets out of this world
alive. You can stay hidden under your bed covers your entire life and
bad things will still happen to good people.

At one company I am familiar with, the chemists are not allowed to use
toluene. It seems the company believes it just too dangerous to be used
by trained professionals.

I suggest you weight the potential dangers from UA against all the
dangers from that glass of red wine. I suspect the alcohol is more
dangerous.

Having said my 2cents, I’m heading home for grilled meat and a beer.


Living on the edge……
Frank Karl



DusevichV-at-umkc.ed
u
To
01/09/2009 03:21 frank_karl-at-lincolnelectric.com
PM cc

Subject
Please respond to [Microscopy] viaWWW: uranyl
DusevichV-at-umkc.ed compounds are alpha emitters only
u












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear Ayten,

As a nuclear engineer you should know better about dangers of radioactivity
(by the way, by training I am a physicist.)

Decay tree says us nothing about these dangers. We should know real levels
of radiation. What can you say about them? Is level of radiation at 5
inches from open bottle with 25 g of uranil acetate much greater then the
one from granite countertop in someone kitchen? I do not know. Since I do
not know, I will be cautious, but just cautious, not paranoid.

Let’s see what World Health Association says:
http://www.who.int/mediacentre/factsheets/fs257/en/

“DEPLETED URANIUM
• On average, approximately 90 µg (micrograms) of uranium
exists in the human body from normal intakes of water, food and air. About
66% is found in the skeleton, 16% in the liver, 8% in the kidneys and 10%
in other tissues. {90 micrograms of uranium, not depleted uranium}
• The uranium remaining after removal of the enriched fraction
contains about 99.8% 238U, 0.2% 235U and 0.001% 234U by mass; this is
referred to as depleted uranium or DU.
• Under most circumstances, use of DU will make a negligible
contribution to the overall natural background levels of uranium in the
environment. Probably the greatest potential for DU exposure will follow
conflict where DU munitions are used.
• Average annual intakes of uranium by adults are estimated to
be about 0.5mg (500 μg) from ingestion of food and water and 0.6 μg from
breathing air.â€Â

So, uranium is everywhere. We should not bother ourselves with decay tree.
If in doubt, we should measure radiation. And, of course, we should
remember about toxicity of uranium. Gloves, fume hoods, proper treatment of
spillage, etc. And when required, we should collect and dispose used
chemicals in proper way.

I beg your pardon if you find my reply a bit harsh. I just followed your
style.

Regards,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
} Sent: Friday, January 09, 2009 1:39 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha
} emitters only
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Dear Alex,
}
} Did you have a chance to check the information from
}
} http://atom.kaeri.re.kr/ton/nuc7.html
}
} Let me construct the decay tree. I'm sure everybody in this
} list can do it but, you keep insisting that "uranyl compounds
} are alpha emitters only" so, I will take the time to do the
} job and post in to the list.
}
} Let's start with U-238 which is the starting element in your compound.
}
} 1) U-238 decays into Th-234 by Alpha decay
}
} 2) Th-234 decays into Pa-234 by Beta decay
}
} 3) Pa-234 decays into U-234 by Beta decay
}
} 4) U-234 decays into Th-230 by Alpha decay
}
} 5) Th-230 decays into Ra-226 by Alpha decay
}
} 6) Ra-226 decays into Rn-222 by Alpha decay
}
} 7) Rn-222 decays into Po-218 by Alpha decay
}
} 8) Po-218 decays into Pb-214 by Alpha decay
}
} 9) Pb-214 decays into Bi-214 by Beta decay
}
} 10) Bi-214 decays into Po-214 by Beta decay
}
} 11) Po-214 decays into Pb-210 by Alpha decay
}
} 12) Pb-210 decays into Bi-210 by Beta decay
}
} 13) Bi-210 decays into Po-210 by Beta decay
}
} 14) Po-210 decays into Pb-206 by Alpha decay
}
} Pb-206 is STABLE so, it is the last element to be produced as
} a result of U-238 radioactive decay.
}
} I have constructed the above decay tree using the information
} from http://atom.kaeri.re.kr/ton/nuc7.html.
} While constructing the above decay tree I have used the
} branch which has the highest branch ratio (above 99% in each case).
}
} I do not understand why you are trying to keep things "under control"?
}
} By the way, I'm a Nuclear Engineer.
}
} One does not even need to be nuclear engineer to understand
} this. Even in high school science classes people learn about
} radioactive decay series e.g. A decays into B and B decays into C...
}
} Best,
} Ayten.
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
}
} On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote:
} }
} } Email: abesenyo-at-ibilabs.com
} } Name: Alex Besenyo PhD
} }
} } Organization: ibilabs
} }
} } Title-Subject: [Filtered] uranyl compounds are alpha emitters only
} }
} } Question: Question:
} }
} } Is it true that the stuff we use has been somehow
} } depleted, so that it isn't as radioactive as "real" uranyl
} } salts? Or is this yet another old wive's tale of EM?!
} }
} } Reply:
} }
} } When we manufacture these compounds we purchase the raw uranium in a
} } depleted state from the government. There is no chance for error
} } here. We do not use natural uranium.
} }
} } This means that the enrichable uranium U-235 has been removed.
} } The then U-238 which only emitts alpha radiation is procesed.
} }
} } The term "depleted" means that U-235 has been removed.
} }
} } If even by the slightest chance that U235 were present then every
} } alarm would go off in our facility because Beta and Gamma radiation
} } is detected.
} }
} } I hope this answers everybodies concerns.
} }
} } Our products are sold exclusively through a distributor network and
} } all of them have been instructed on this information.
} }
} } I only responded when I saw the original post and I had to respond
} } before it got out of control.
} }
} } Sincerely
} } Alex Besenyo PhD
} }
} }
} }
}
} ==============================Original
} Headers==============================
} 28, 37 -- From celikaktas-at-gmail.com Fri Jan 9 01:38:48 2009
} 28, 37 -- Received: from mail-bw0-f12.google.com
} (mail-bw0-f12.google.com [209.85.218.12])
} 28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n097cllZ007406
} 28, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan
} 2009 01:38:48 -0600
} 28, 37 -- Received: by bwz5 with SMTP id 5so20323150bwz.18
} 28, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08
} Jan 2009 23:38:46 -0800 (PST)
} 28, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 28, 37 -- d=gmail.com; s=gamma;
} 28, 37 --
} h=domainkey-signature:received:received:message-id:date:from:to
} 28, 37 -- :subject:in-reply-to:mime-version:content-type
} 28, 37 --
} :content-transfer-encoding:content-disposition:references;
} 28, 37 -- bh=x3VBln30706ul3mo8rzkKk8tdVV0mnTtpGId5uaYeEM=;
} 28, 37 --
} b=qBVYz0biX1/wFlDLibyks38NncsgFjqElzGSuPXOiX+ZCB902M5i3lsMmCryurouqZ
} 28, 37 --
} q2vvqo4qq+LQCiFwGfBQltrqfo1jiWYiWnPMwvN3V4b9MDoZd/yjgF76rgbzKDtibrjg
} 28, 37 -- Ob/1zXIpi/2mHaPBWSu4mzAG29Fqx67ZL1YFg=
} 28, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 28, 37 -- d=gmail.com; s=gamma;
} 28, 37 --
} h=message-id:date:from:to:subject:in-reply-to:mime-version
} 28, 37 --
} :content-type:content-transfer-encoding:content-disposition
} 28, 37 -- :references;
} 28, 37 --
} b=DNSaPkpBjjjni4T+G+pPUuxo3M1nntLysfMKUnfOWQUEElcsWGJ3PmM4uVFQBbtTpT
} 28, 37 --
} g+IO1lpZUOd3KJn43j3ed/pgCrIp1p0oTRjteC1sfQN2njKF2xkzfFZtTt2rNMbo4jBE
} 28, 37 -- /h849iT1rgdv5cvNLmKbW91LhMbKuR486Rzbg=
} 28, 37 -- Received: by 10.103.217.7 with SMTP id
} u7mr1380019muq.125.1231486725631;
} 28, 37 -- Thu, 08 Jan 2009 23:38:45 -0800 (PST)
} 28, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan
} 2009 23:38:45 -0800 (PST)
} 28, 37 -- Message-ID:
} {1075c5c10901082338y14d2b189m26ca5f198f5a9454-at-mail.gmail.com}
} 28, 37 -- Date: Fri, 9 Jan 2009 09:38:45 +0200
} 28, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com}
} 28, 37 -- To: abesenyo-at-ibilabs.com, microscopy
} {Microscopy-at-microscopy.com}
} 28, 37 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds
} are alpha emitters only
} 28, 37 -- In-Reply-To: {200901090027.n090RYH1022494-at-ns.microscopy.com}
} 28, 37 -- MIME-Version: 1.0
} 28, 37 -- Content-Type: text/plain; charset=UTF-8
} 28, 37 -- Content-Transfer-Encoding: 7bit
} 28, 37 -- Content-Disposition: inline
} 28, 37 -- References: {200901090027.n090RYH1022494-at-ns.microscopy.com}
} ==============================End of -
} Headers==============================
}
}


==============================Original
Headers==============================
14, 25 -- From DusevichV-at-umkc.edu Fri Jan 9 14:13:53 2009
14, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu
[134.193.143.155])
14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n09KDquJ008557
14, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009
14:13:52 -0600
14, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 25 -- Fri, 9 Jan 2009 14:13:44 -0600
14, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 25 -- Content-class: urn:content-classes:message
14, 25 -- MIME-Version: 1.0
14, 25 -- Content-Type: text/plain;
14, 25 -- charset="UTF-8"
14, 25 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha
emitters only
14, 25 -- Date: Fri, 9 Jan 2009 14:13:44 -0600
14, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AA-at-KC-MSX1.kc.umkc.edu}
14, 25 -- In-Reply-To: {200901090739.n097dQZk008263-at-ns.microscopy.com}
14, 25 -- X-MS-Has-Attach:
14, 25 -- X-MS-TNEF-Correlator:
14, 25 -- Thread-Topic: [Microscopy] Re: viaWWW: uranyl compounds are alpha
emitters only
14, 25 -- thread-index: AclyLWbfA0iBUCGrQXeRBZzilk1+5QAaS2kQ
14, 25 -- References: {200901090739.n097dQZk008263-at-ns.microscopy.com}
14, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
14, 25 -- To: {Microscopy-at-microscopy.com}
14, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:44.0274 (UTC) FILETIME=
[C59B9B20:01C97296]
14, 25 -- Content-Transfer-Encoding: 8bit
14, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id
n09KDquJ008557
==============================End of -
Headers==============================

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
38, 24 -- From frank_karl-at-lincolnelectric.com Fri Jan 9 14:31:19 2009
38, 24 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
38, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KVJT7000814
38, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:31:19 -0600
38, 24 -- In-Reply-To: {200901092021.n09KLUr1031349-at-ns.microscopy.com}
38, 24 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters only
38, 24 -- To: DusevichV-at-umkc.edu, Microscopy-at-microscopy.com
38, 24 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
38, 24 -- Message-ID: {OFE5145C76.CBE47B69-ON85257539.00708B12-85257539.0070B254-at-lincolnelectric.com}
38, 24 -- Date: Fri, 9 Jan 2009 15:31:02 -0500
38, 24 -- From: Frank_Karl-at-lincolnelectric.com
38, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM,
38, 24 -- CD-MIME complete at 01/09/2009 03:31:04 PM,
38, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM,
38, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM,
38, 24 -- Serialize complete at 01/09/2009 03:31:04 PM
38, 24 -- MIME-Version: 1.0
38, 24 -- Content-Type: text/plain;
38, 24 -- charset="UTF-8"
38, 24 -- Content-Transfer-Encoding: 8bit
38, 24 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n09KVJT7000814
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Fri, 9 Jan 2009 14:32:36 -0600
Subject: [Microscopy] Re: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kristen

There is no compelling reason to use cacodylate buffers for an
introductory class. If you need to add calcium to the fix you can use
HEPES or PIPES, plenty of literature on these.
Cacodylate was very convenient because it was a one salt buffer and Ca
ions did not precipitate. Hazardous waste disposal concerns have made
its use difficult to justify.

Geoff

kamlennon-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Again Listers,
}
} Thanks to all of you who have responded to my recent query. I'm sure that I will have more as time passes, and I appreciate the fantastic response that I've had from the list.
}
} Since we are on the topic of the dangers of EM work (the uranyl acetate thread), I have a question about cacodylate buffer. I'm getting set to teach an introductory EM course for biologists to undergraduates. Having interviewed two of my three students during the previous semester, I know that I will be starting very much at zero. They had no to little knowledge of what "EM" is or can be used for before I spoke with them - they are exploring this new class. My plan is to take them through the preparation of plant, animal, and some sort of micro sample via traditional chemical fixation methods and keep it as simple as possible. I am inclined to steer clear of cacodylate buffer due to its toxicity and because they have enough to deal with already, and stick with phosphate buffer. However, I have noticed that most if not all of the animal tissue protocols I've been perusing use cac buffer. Is there any reason why I should keep it in the protocol?
}
} Thanks for your advice.
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} k.lennon-at-frostburg.edu
}
}
}
}
} ==============================Original Headers==============================
} 7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009
} 7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09JvEx0012745
} 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 13:57:14 -0600
} 7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9 Jan 2009 19:57:14 -0000
} 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 7, 20 -- s=s1024; d=yahoo.com;
} 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
} 7, 20 -- b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=;
} 7, 20 -- X-YMail-OSG: LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg--
} 7, 20 -- Received: from [96.239.149.81] by web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009 11:57:13 PST
} 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
} 7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST)
} 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 7, 20 -- Reply-To: kamlennon-at-yahoo.com
} 7, 20 -- Subject: Cac buffer and undergrads - chancy?
} 7, 20 -- To: Microscopy-at-Microscopy.Com
} 7, 20 -- MIME-Version: 1.0
} 7, 20 -- Content-Type: text/plain; charset=us-ascii
} 7, 20 -- Message-ID: {982974.76126.qm-at-web84004.mail.mud.yahoo.com}
} ==============================End of - Headers==============================
}
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff-at-umdnj.edu
**********************************************




==============================Original Headers==============================
9, 28 -- From mcauliff-at-umdnj.edu Fri Jan 9 14:32:36 2009
9, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09KWaoa003266
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:32:36 -0600
9, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1])
9, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 80F2F4C05F
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:32:35 -0500 (EST)
9, 28 -- Received: from umdnj.edu (unknown [10.32.15.102])
9, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 1757550C155
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:20:18 -0500 (EST)
9, 28 -- Received: from ([10.32.15.167])
9, 28 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.4223090;
9, 28 -- Fri, 09 Jan 2009 15:20:15 -0500
9, 28 -- MIME-version: 1.0
9, 28 -- Content-transfer-encoding: 7BIT
9, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
9, 28 -- Received: from [127.0.0.1] ([10.32.15.102])
9, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
9, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KD800F280HR8880-at-umduwc01.umdnj.edu} for
9, 28 -- microscopy-at-microscopy.com; Fri, 09 Jan 2009 15:20:15 -0500 (EST)
9, 28 -- Message-id: {4967B1C9.50300-at-umdnj.edu}
9, 28 -- Date: Fri, 09 Jan 2009 15:21:29 -0500
9, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
9, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
9, 28 -- To: kamlennon-at-yahoo.com, microscopy-at-microscopy.com
9, 28 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
9, 28 -- References: {200901091958.n09Jw4H3015451-at-ns.microscopy.com}
9, 28 -- In-reply-to: {200901091958.n09Jw4H3015451-at-ns.microscopy.com}
==============================End of - Headers==============================




From: PWebster-at-hei.org
Date: Fri, 9 Jan 2009 14:45:35 -0600
Subject: [Microscopy] Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I have always found it strange that there is so much concern about the use
of cacodylate in the EM laboratory. I concede that it is dangerous when
handled incorrectly. However, I would think that phosphate buffer containing
2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of
osmium tetroxide. At what point should students start taking responsibility
for handling chemicals safely and when do the trainers bite the bullet and
make sure the training is adequate?

During my training I remember very clearly how I was taught how to handle
pathogenic bacteria and viruses. The instructions were very clear and very
strict, and followed the same basic rules of how I was trained to handle
radioactive material. Basically the instructions were that these agents can
make you sick and even kill you if you don't follow my instructions, so you
have to handle them as follows....

When I moved into electron microscopy, the training I was given to prepare
biological specimens consisted of being given access to a fridge full of
chemicals and a written protocol. The brown spots on my hands appeared after
a couple of hours but my corneas clouded over in about 30 min of my using
the osmium tetroxide. I had been left completely unsupervised to handle
these chemicals without any prior warning of their dangers. Interestingly,
when my supervisor found out about my use of the osmium tetroxide, and what
it had done to me, he blamed me!

With proper training, the chemicals we use in the EM lab are basically very
safe. We use small amounts of them and store them in closed cabinets so they
should not affect our health in any way.

Part of a good laboratory training course is teaching users how to handle
toxic chemicals and how to pipette solutions without creating aerosols.
Making sure that protective gloves are used correctly is another important
aspect of this training.

If we train correctly, then it should be sufficient to warn students that
they are handling materials designed to chemically alter biological
material. At this point, I usually remind them that they are made of
biological material too.

Happy New Year
(My New Years Resolution is very high!)

Best regards,

Paul Webster.

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org







==============================Original Headers==============================
16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009
16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KjYwS028110
16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 14:45:35 -0600
16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ;
16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000
16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119
16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800
16, 18 -- Subject: Cac buffer and undergrads - chancy?
16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org}
16, 18 -- To: {Microscopy-at-Microscopy.Com}
16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org}
16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy?
16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw==
16, 18 -- Mime-version: 1.0
16, 18 -- Content-type: text/plain;
16, 18 -- charset="US-ASCII"
16, 18 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Fri, 9 Jan 2009 15:18:40 -0600
Subject: [Microscopy] Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I naturally agree with Paul that with proper training that all the
chemicals used in an EM are safe but that doesn't make it a good idea to
use them indiscriminately. The comparison to bacteria and viruses is a
red herring since those are typically the subject of interest as opposed
to the selection of a buffer which is discretionary. Microscopists and
other scientists inevitably generate hazardous waste but it is incumbent
on us not to do so unless there is a scientific reason that it is the
only way to do the experiment.

It is true that glutaraldehyde in phosphate buffer is dangerous. But
glutaraldehyde in cacodylate buffer is more dangerous. Accidental
exposure would mean exposure to two dangerous chemicals. In addition,
adding acid to glutaraldehyde in phosphate will change the pH but not
release arsenic gas. Many studies have shown the Good buffers to be
suitable for LM and EM studies. I see no reason for any microscopist to
cling to the use of cacodylate.

Paul's own horror story of poor training and supervision is further
evidence of why one should minimize all unnecessary risks. I doubt any
trained scientist would any chemical they don't fully understand outside
of the hood and without gloves - that's a beginner's mistake. Unless one
can guarantee being present at every step of a new student's processing,
it would only be prudent to use the least toxic formulations. With
experience, those students will be able to judge the risks they wish to
take.


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
Sent: Friday, January 09, 2009 2:46 PM
To: Phillips, Thomas E.

Dear All,

I have always found it strange that there is so much concern about the
use
of cacodylate in the EM laboratory. I concede that it is dangerous when
handled incorrectly. However, I would think that phosphate buffer
containing
2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of
osmium tetroxide. At what point should students start taking
responsibility
for handling chemicals safely and when do the trainers bite the bullet
and
make sure the training is adequate?

During my training I remember very clearly how I was taught how to
handle
pathogenic bacteria and viruses. The instructions were very clear and
very
strict, and followed the same basic rules of how I was trained to handle
radioactive material. Basically the instructions were that these agents
can
make you sick and even kill you if you don't follow my instructions, so
you
have to handle them as follows....

When I moved into electron microscopy, the training I was given to
prepare
biological specimens consisted of being given access to a fridge full of
chemicals and a written protocol. The brown spots on my hands appeared
after
a couple of hours but my corneas clouded over in about 30 min of my
using
the osmium tetroxide. I had been left completely unsupervised to handle
these chemicals without any prior warning of their dangers.
Interestingly,
when my supervisor found out about my use of the osmium tetroxide, and
what
it had done to me, he blamed me!

With proper training, the chemicals we use in the EM lab are basically
very
safe. We use small amounts of them and store them in closed cabinets so
they
should not affect our health in any way.

Part of a good laboratory training course is teaching users how to
handle
toxic chemicals and how to pipette solutions without creating aerosols.
Making sure that protective gloves are used correctly is another
important
aspect of this training.

If we train correctly, then it should be sufficient to warn students
that
they are handling materials designed to chemically alter biological
material. At this point, I usually remind them that they are made of
biological material too.

Happy New Year
(My New Years Resolution is very high!)

Best regards,

Paul Webster.

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org







==============================Original
Headers==============================
16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009
16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54]
(may be forged))
16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n09KjYwS028110
16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009
14:45:35 -0600
16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by
hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV
;
16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000
16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119
16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800
16, 18 -- Subject: Cac buffer and undergrads - chancy?
16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org}
16, 18 -- To: {Microscopy-at-Microscopy.Com}
16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org}
16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy?
16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw==
16, 18 -- Mime-version: 1.0
16, 18 -- Content-type: text/plain;
16, 18 -- charset="US-ASCII"
16, 18 -- Content-transfer-encoding: 7bit
==============================End of -
Headers==============================



==============================Original Headers==============================
29, 29 -- From PhillipsT-at-missouri.edu Fri Jan 9 15:18:40 2009
29, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
29, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09LIdVT010691
29, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:18:39 -0600
29, 29 -- X-IronPort-Anti-Spam-Filtered: true
29, 29 -- X-IronPort-Anti-Spam-Result: ApoEAGhNZ0nRauUp/2dsb2JhbADFJQEJhTmFVQGEP4Ev
29, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
29, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Jan 2009 15:18:38 -0600
29, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
29, 29 -- Fri, 9 Jan 2009 15:18:38 -0600
29, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
29, 29 -- Content-class: urn:content-classes:message
29, 29 -- MIME-Version: 1.0
29, 29 -- Content-Type: text/plain;
29, 29 -- charset="us-ascii"
29, 29 -- Subject: RE: [Microscopy] Cac buffer and undergrads - chancy?
29, 29 -- Date: Fri, 9 Jan 2009 15:18:37 -0600
29, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD640-at-UM-XMAIL06.um.umsystem.edu}
29, 29 -- In-Reply-To: {200901092046.n09KkNk7029228-at-ns.microscopy.com}
29, 29 -- X-MS-Has-Attach:
29, 29 -- X-MS-TNEF-Correlator:
29, 29 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy?
29, 29 -- thread-index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQ
29, 29 -- References: {200901092046.n09KkNk7029228-at-ns.microscopy.com}
29, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
29, 29 -- To: {PWebster-at-hei.org} , {Microscopy-at-microscopy.com}
29, 29 -- X-OriginalArrivalTime: 09 Jan 2009 21:18:38.0694 (UTC) FILETIME=[D6DCF060:01C9729F]
29, 29 -- Content-Transfer-Encoding: 8bit
29, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09LIdVT010691
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Fri, 9 Jan 2009 15:27:19 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I disagree with the assumption that depleted uranium (DU) is not
radioactive and the implication by the word "depleted" that all the
radioactivity has been removed. Here's why.

I had 12 pounds of depleted UAc and a calibrated and certified "pancake"
Geiger counter detector. It had no problem detecting background cosmic
radiation and it had an up to date certification sticker on it. That
amount of DUAc in those containers pegged my Geiger counter from three feet
away. The one pound bottles were brown glass bottles, inside a plastic
bag, inside a "tin" shipping can, and had labels that said, "(depleted
uranium)" on them. So the counted radiation had to be gamma but U does not
emit gamma radiation.

It is the impurities from the decay of U that generate the gamma emitters.
I have no doubt that some of the posters think their materials are not
radioactive and their supplier's material may not be. So we now have two
schools of thought. It's not radioactive and there is radioactivity
present. The only way to know for sure what you have and get a hint of the
history of the manufacturing, is to take a reading on the purchased DUAc
salt with a good quality Geiger counter and see what you get for a reading.
My EH&S and safety people were totally shocked at the DUAc readings and
said, "But this was made from depleted uranium. It's not radioactive." I
relied, "Looks pretty radioactive to me." Like me, they believed the
Geiger counter readings and not the MSDS sheets that didn't address the
gamma emitting impurities, i.e. the amount of impurities from decay.

IMO, the phrase 'depleted uranium' is misleading. There is a shipping
exemption on this radioactive material, I was told. If the amount is one
ounce or less, you don't have to label it or ship it as radioactive. My
shipping clerk refused to ship any amount. So just because the bottle or
packaging you received does not say "radioactive material", that does not
mean small amounts are not radioactive. I think that's where the EM myth
of not being radioactive might comes in. How do you know every last U-235
atom was removed and the uranium was zone refined and/or chemically
purified? You don't. It doesn't say any of that on the bottle(s). Just
measure the gamma radiation.

Paul Beauregard
Senior Research Associate



At 06:20 PM 1/8/09 -0600, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
8, 28 -- From beaurega-at-westol.com Fri Jan 9 15:27:19 2009
8, 28 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09LRJTJ024332
8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:27:19 -0600
8, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
8, 28 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id n09LREO4007130
8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 16:27:15 -0500
8, 28 -- Received: (qmail 9427 invoked by uid 89); 9 Jan 2009 21:27:08 -0000
8, 28 -- Received: from pitts-69-72-21-71.dynamic-dialup.coretel.net (HELO running) (69.72.21.71)
8, 28 -- by mail.winbeam.com with SMTP; 9 Jan 2009 21:27:08 -0000
8, 28 -- Message-Id: {3.0.6.32.20090109162749.00893c10-at-pop3.norton.antivirus}
8, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus
8, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
8, 28 -- Date: Fri, 09 Jan 2009 16:27:49 -0500
8, 28 -- To: microscopy-at-microscopy.com
8, 28 -- From: Beaurega {beaurega-at-westol.com}
8, 28 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters
8, 28 -- only
8, 28 -- Mime-Version: 1.0
8, 28 -- Content-Type: text/plain; charset="us-ascii"
8, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
8, 28 -- X-Winbeam-MailScanner-ID: n09LREO4007130
8, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
8, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted),
8, 28 -- SpamAssassin (not cached, score=-1.9, required 4, autolearn=not spam,
8, 28 -- AWL 0.10, BAYES_00 -2.00)
8, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
8, 28 -- X-Winbeam-MailScanner-Watermark: 1232141236.05045-at-/cuIVPDK8713eRRY8mXR4w
==============================End of - Headers==============================




From: kleopullin-at-pacbell.net
Date: Fri, 9 Jan 2009 15:52:21 -0600
Subject: [Microscopy] Re: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In our introductory and advanced courses we used phosphate buffers for animal tissue. My classmates and I all got knock-you-dead micrographs, better than the ones in our textbooks, of liver, kidney, muscle, brain, gills, lung, and etc., using phosphate buffers, so I think they can be used with animal tissue to obtain good results.

We're required to read the MSDS before using a chemical, and we're tested throughout both semesters on the safe handling of all EM chemicals, on ALL tests, even chemicals we don't used, from day one in lab.

On our first project, first semester, advanced students handled the OsO4 for us, and we made only the buffers and resins. Later in that semester we were handling and even preparing OsO4, and other chemicals, when we needed more or different concentrations, and if our teacher thought we could do so safely.

For our individual projects, later on, we can use the chemical fixation protocol of our choice--we write these up and submit them weeks before the project.

I used cacodylate buffer for my final project for my advanced biological EM course, and it had to be made from scratch. I worked with a partner, and we used a lab area when other students were absent, and had proper changes of gloves, fume hoods and scale arrangements that would not aerosolize anything, clean tools ready to limit handling time, etc., etc. We figured out the logistics and safe handling by ourselves from experience, questions, and the MSDS's.

We also had a great teacher who watched us like hawks before allowing us to mix our own chemicals and told us right away if we handled something incorrectly. Also, we watch and correct each other before our teacher gets the chance. Lab accidents caused by carelessness are a huge waste of time.

Kleo Pullin






--- On Fri, 1/9/09, kamlennon-at-yahoo.com {kamlennon-at-yahoo.com} wrote:

} From: kamlennon-at-yahoo.com {kamlennon-at-yahoo.com}
} Subject: [Microscopy] Cac buffer and undergrads - chancy?
} To: KLeoPullin-at-pacbell.net
} Date: Friday, January 9, 2009, 12:01 PM
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Again Listers,
}
} Thanks to all of you who have responded to my recent query.
} I'm sure that I will have more as time passes, and I
} appreciate the fantastic response that I've had from the
} list.
}
} Since we are on the topic of the dangers of EM work (the
} uranyl acetate thread), I have a question about cacodylate
} buffer. I'm getting set to teach an introductory EM
} course for biologists to undergraduates. Having interviewed
} two of my three students during the previous semester, I
} know that I will be starting very much at zero. They had no
} to little knowledge of what "EM" is or can be used
} for before I spoke with them - they are exploring this new
} class. My plan is to take them through the preparation of
} plant, animal, and some sort of micro sample via traditional
} chemical fixation methods and keep it as simple as possible.
} I am inclined to steer clear of cacodylate buffer due to its
} toxicity and because they have enough to deal with already,
} and stick with phosphate buffer. However, I have noticed
} that most if not all of the animal tissue protocols I've
} been perusing use cac buffer. Is there any reason why I
} should keep it in the protocol?
}
} Thanks for your advice.
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} k.lennon-at-frostburg.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009
} 7, 20 -- Received: from web84004.mail.mud.yahoo.com
} (web84004.mail.mud.yahoo.com [68.142.206.174])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id n09JvEx0012745
} 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan
} 2009 13:57:14 -0600
} 7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9
} Jan 2009 19:57:14 -0000
} 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 7, 20 -- s=s1024; d=yahoo.com;
} 7, 20 --
} h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
} 7, 20 --
} b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=;
} 7, 20 -- X-YMail-OSG:
} LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg--
} 7, 20 -- Received: from [96.239.149.81] by
} web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009
} 11:57:13 PST
} 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
} 7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST)
} 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 7, 20 -- Reply-To: kamlennon-at-yahoo.com
} 7, 20 -- Subject: Cac buffer and undergrads - chancy?
} 7, 20 -- To: Microscopy-at-Microscopy.Com
} 7, 20 -- MIME-Version: 1.0
} 7, 20 -- Content-Type: text/plain; charset=us-ascii
} 7, 20 -- Message-ID:
} {982974.76126.qm-at-web84004.mail.mud.yahoo.com}
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
14, 20 -- From kleopullin-at-pacbell.net Fri Jan 9 15:52:21 2009
14, 20 -- Received: from web83405.mail.sp1.yahoo.com (web83405.mail.sp1.yahoo.com [69.147.64.53])
14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09LqKsh006024
14, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 15:52:21 -0600
14, 20 -- Received: (qmail 85987 invoked by uid 60001); 9 Jan 2009 21:52:20 -0000
14, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 20 -- s=s1024; d=pacbell.net;
14, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
14, 20 -- b=6BGoEGZRiX+t35SsP/ZxdZwTKRcM/eFcrmMo1JyjALCkBsHqApT/5EZqJzjEZ5bCkyUsAPnUQiqztd2oHD+tITdKvXJOC5qBOOgAtzmogoO98XYNlm/vllX758+/6+v2picHm9h4SmFHDJVat4W9mbouXg6062oJnZOz2XHrmdI=;
14, 20 -- X-YMail-OSG: NdCCuD0VM1nODQ9p9gMVoVEdgF8YuA4Qjrm0TyceSJkdBxPoCeIqdeKIThmPxEhjBwfDH6WqSjV1gUcfyJ8GELMxiyC5xnNnEEKy.Oi_ywwTH5Kv92APyUsfSttvFbaPUeoaEqeZqghE8nvhJ0MxbZz9LbFZ4UuQsP_Kmh1pJOQq4F3zj28o_vhDTlka
14, 20 -- Received: from [69.225.11.246] by web83405.mail.sp1.yahoo.com via HTTP; Fri, 09 Jan 2009 13:52:20 PST
14, 20 -- X-Mailer: YahooMailWebService/0.7.218.2
14, 20 -- Date: Fri, 9 Jan 2009 13:52:20 -0800 (PST)
14, 20 -- From: Kleo Pullin {kleopullin-at-pacbell.net}
14, 20 -- Reply-To: kleopullin-at-pacbell.net
14, 20 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
14, 20 -- To: Microscopy-at-Microscopy.Com
14, 20 -- MIME-Version: 1.0
14, 20 -- Content-Type: text/plain; charset=us-ascii
14, 20 -- Message-ID: {479577.85874.qm-at-web83405.mail.sp1.yahoo.com}
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 9 Jan 2009 16:14:35 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 9, 2009, at 1:27 PM, beaurega-at-westol.com wrote:

} I disagree with the assumption that depleted uranium (DU) is not
} radioactive and the implication by the word "depleted" that all the
} radioactivity has been removed.


Dear Paul,
Depleted U is natural U from which almost all U235 has been removed.
Just because U238 does not sustain a fission chain reaction does not
mean that it is not radioactive. I would refer anyone who believes
that U238 is not radioactive to any book or web site where
radioactivity is discussed, or, as you say "Just measure the gamma
radiation."
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Fri Jan 9 16:14:35 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09MEZVe021473
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 16:14:35 -0600
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 36AD9328B97
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:14:35 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 40540328DC7
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:14:34 -0800 (PST)
6, 22 -- Message-Id: {ADAF5D19-031F-46B8-AA19-007568467A9C-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200901092127.n09LRPfo024462-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
6, 22 -- Date: Fri, 9 Jan 2009 14:14:33 -0800
6, 22 -- References: {200901092127.n09LRPfo024462-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: PWebster-at-hei.org
Date: Fri, 9 Jan 2009 17:15:17 -0600
Subject: [Microscopy] Re: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

My message about cacodylate was aimed at stimulating a continuation of this
discussion, not to advocate the use of cacodylate buffer. However, the
toxicity of the compound should not be the only reason for not choosing to
use it. The choice of buffer will also depend on the end result needed.

My point in the first message was to point out that with proper training it
is possible to handle almost any of the chemicals we encounter in a safe
way.

One important caveat that I would add about using phosphate buffer in the
primary fixative is that when mixed with glutaraldehyde and osmium tetroxide
it can form a fine precipitate in the cells.

If using phosphate buffer, and there are plenty of historical references
where it was used successfully for TEM, make sure the cells or tissues are
washed well after glutaraldehyde fixation and before immersion in osmium
tetroxide.

The choice of buffers for EM is another subject that deserves a long
discussion thread. Tom Phillips suggests the use of Good buffers as an
alternative to cacodylate. However, PIPES and HEPES will preserve tissues
and cells in very different ways when compared with each other, as well as
with cacodylate or phosphate buffers. This is partially due to the amount of
extraction that occurs. In addition, PIPES buffer is often used at a pH
where it has almost no buffering capacity, HEPES can preserve tissues so
well that there is almost no extraction of cellular material, which results
in virtually no specimen contrast and TRIS buffer may not work at all due to
its ability to react with the aldehyde. In the end, fixation recipe changes
should be approached with some caution.

Best regards,

Paul.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


} From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
} Date: Fri, 9 Jan 2009 15:18:37 -0600
} To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com}
} Subject: RE: [Microscopy] Cac buffer and undergrads - chancy?
}
} I naturally agree with Paul that with proper training that all the
} chemicals used in an EM are safe but that doesn't make it a good idea to
} use them indiscriminately. The comparison to bacteria and viruses is a
} red herring since those are typically the subject of interest as opposed
} to the selection of a buffer which is discretionary. Microscopists and
} other scientists inevitably generate hazardous waste but it is incumbent
} on us not to do so unless there is a scientific reason that it is the
} only way to do the experiment.
}
} It is true that glutaraldehyde in phosphate buffer is dangerous. But
} glutaraldehyde in cacodylate buffer is more dangerous. Accidental
} exposure would mean exposure to two dangerous chemicals. In addition,
} adding acid to glutaraldehyde in phosphate will change the pH but not
} release arsenic gas. Many studies have shown the Good buffers to be
} suitable for LM and EM studies. I see no reason for any microscopist to
} cling to the use of cacodylate.
}
} Paul's own horror story of poor training and supervision is further
} evidence of why one should minimize all unnecessary risks. I doubt any
} trained scientist would any chemical they don't fully understand outside
} of the hood and without gloves - that's a beginner's mistake. Unless one
} can guarantee being present at every step of a new student's processing,
} it would only be prudent to use the least toxic formulations. With
} experience, those students will be able to judge the risks they wish to
} take.
}
}
} Thomas E. Phillips, Ph.D
} Professor of Biological Sciences
} Chair, MU Faculty Council
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
} 573-882-4712 (office)
} 573-882-0123 (fax)
} phillipst-at-missouri.edu
}
} http://www.biology.missouri.edu/faculty/phillips.html
} http://www.biotech.missouri.edu/mcc/
}
} -----Original Message-----
} From: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
} Sent: Friday, January 09, 2009 2:46 PM
} To: Phillips, Thomas E.
} Subject: [Microscopy] Cac buffer and undergrads - chancy?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} Dear All,
}
} I have always found it strange that there is so much concern about the
} use
} of cacodylate in the EM laboratory. I concede that it is dangerous when
} handled incorrectly. However, I would think that phosphate buffer
} containing
} 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of
} osmium tetroxide. At what point should students start taking
} responsibility
} for handling chemicals safely and when do the trainers bite the bullet
} and
} make sure the training is adequate?
}
} During my training I remember very clearly how I was taught how to
} handle
} pathogenic bacteria and viruses. The instructions were very clear and
} very
} strict, and followed the same basic rules of how I was trained to handle
} radioactive material. Basically the instructions were that these agents
} can
} make you sick and even kill you if you don't follow my instructions, so
} you
} have to handle them as follows....
}
} When I moved into electron microscopy, the training I was given to
} prepare
} biological specimens consisted of being given access to a fridge full of
} chemicals and a written protocol. The brown spots on my hands appeared
} after
} a couple of hours but my corneas clouded over in about 30 min of my
} using
} the osmium tetroxide. I had been left completely unsupervised to handle
} these chemicals without any prior warning of their dangers.
} Interestingly,
} when my supervisor found out about my use of the osmium tetroxide, and
} what
} it had done to me, he blamed me!
}
} With proper training, the chemicals we use in the EM lab are basically
} very
} safe. We use small amounts of them and store them in closed cabinets so
} they
} should not affect our health in any way.
}
} Part of a good laboratory training course is teaching users how to
} handle
} toxic chemicals and how to pipette solutions without creating aerosols.
} Making sure that protective gloves are used correctly is another
} important
} aspect of this training.
}
} If we train correctly, then it should be sufficient to warn students
} that
} they are handling materials designed to chemically alter biological
} material. At this point, I usually remind them that they are made of
} biological material too.
}
} Happy New Year
} (My New Years Resolution is very high!)
}
} Best regards,
}
} Paul Webster.
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} (213) 273 8026
} pwebster-at-hei.org
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009
} 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54]
} (may be forged))
} 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n09KjYwS028110
} 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009
} 14:45:35 -0600
} 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by
} hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV
} ;
} 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000
} 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119
} 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800
} 16, 18 -- Subject: Cac buffer and undergrads - chancy?
} 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org}
} 16, 18 -- To: {Microscopy-at-Microscopy.Com}
} 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org}
} 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy?
} 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw==
} 16, 18 -- Mime-version: 1.0
} 16, 18 -- Content-type: text/plain;
} 16, 18 -- charset="US-ASCII"
} 16, 18 -- Content-transfer-encoding: 7bit
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
12, 20 -- From PWebster-at-hei.org Fri Jan 9 17:15:16 2009
12, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09NFGaA004403
12, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 17:15:16 -0600
12, 20 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ;
12, 20 -- Fri, 9 Jan 2009 23:15:15 +0000
12, 20 -- User-Agent: Microsoft-Entourage/12.15.0.081119
12, 20 -- Date: Fri, 09 Jan 2009 15:15:11 -0800
12, 20 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
12, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
12, 20 -- To: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} ,
12, 20 -- {Microscopy-at-microscopy.com}
12, 20 -- Message-ID: {C58D1A7F.1FB70%PWebster-at-hei.org}
12, 20 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy?
12, 20 -- Thread-Index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQAASC0Ww=
12, 20 -- In-Reply-To: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD640-at-UM-XMAIL06.um.umsystem.edu}
12, 20 -- Mime-version: 1.0
12, 20 -- Content-type: text/plain;
12, 20 -- charset="US-ASCII"
12, 20 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: rp_seifi-at-yahoo.com
Date: Fri, 9 Jan 2009 17:30:58 -0600
Subject: [Microscopy] viaWWW: FISH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PIPES has a pKa of 6.8 which would make it a suitable buffer for 6.3 -
7.3 (and since aldehydes fixation tends to acidify the solution, it
would be good at 7.4 also!). Cacodylate has a pKa of 6.2. I guess it
depends on what pH you are using for your aldehydes fix but most people
use something closer to 7 so I don't see how PIPES pKa is inferior to
cacodylate.



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


-----Original Message-----
X-from: Webster, Paul [mailto:PWebster-at-hei.org]
Sent: Friday, January 09, 2009 5:15 PM
To: Phillips, Thomas E.; Microscopy-at-microscopy.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rp_seifi-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rp_seifi-at-yahoo.com
Name: Reza Pourseify

Organization: Mehr Inst.

Title-Subject: [Filtered] FISH

Question: Hello everybody
I have a problem in my microscope setting.
The line of light is biased to lefthand side in it for some zoom degrees.
How can i correct it.
It is a Nikon, E600 (fluorescence microscope with visible light
harware and a condenser)
Thank you for attention...


Login Host: 79.127.31.226
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Jan 9 17:30:58 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09NUttD031425
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 17:30:57 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c58d8ea21813-at-[206.69.208.22]}
7, 11 -- Date: Fri, 9 Jan 2009 17:30:55 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: rp_seifi-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: FISH
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: PWebster-at-hei.org
Date: Fri, 9 Jan 2009 17:38:47 -0600
Subject: [Microscopy] Re: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again,

One last time - I am not advocating the use of cacodylate and I do agree
that cacodylate as generally used in EM has less buffering capacity than
PIPES. I don't think I ever said that one buffer was inferior to another,
just that choices should be made on the end result, not on whether a
compound was toxic or not.

Regards,

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


} From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
} Date: Fri, 9 Jan 2009 17:22:17 -0600
} To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com}
} Subject: RE: [Microscopy] Cac buffer and undergrads - chancy?
}
} PIPES has a pKa of 6.8 which would make it a suitable buffer for 6.3 -
} 7.3 (and since aldehydes fixation tends to acidify the solution, it
} would be good at 7.4 also!). Cacodylate has a pKa of 6.2. I guess it
} depends on what pH you are using for your aldehydes fix but most people
} use something closer to 7 so I don't see how PIPES pKa is inferior to
} cacodylate.
}
}
}
} Thomas E. Phillips, Ph.D
} Professor of Biological Sciences
} Chair, MU Faculty Council
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
} 573-882-4712 (office)
} 573-882-0123 (fax)
} phillipst-at-missouri.edu
}
} http://www.biology.missouri.edu/faculty/phillips.html
} http://www.biotech.missouri.edu/mcc/
}
}
} -----Original Message-----
} From: Webster, Paul [mailto:PWebster-at-hei.org]
} Sent: Friday, January 09, 2009 5:15 PM
} To: Phillips, Thomas E.; Microscopy-at-microscopy.com
} Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
}
} Hi All,
}
} My message about cacodylate was aimed at stimulating a continuation of
} this
} discussion, not to advocate the use of cacodylate buffer. However, the
} toxicity of the compound should not be the only reason for not choosing
} to
} use it. The choice of buffer will also depend on the end result needed.
}
} My point in the first message was to point out that with proper training
} it
} is possible to handle almost any of the chemicals we encounter in a safe
} way.
}
} One important caveat that I would add about using phosphate buffer in
} the
} primary fixative is that when mixed with glutaraldehyde and osmium
} tetroxide
} it can form a fine precipitate in the cells.
}
} If using phosphate buffer, and there are plenty of historical references
} where it was used successfully for TEM, make sure the cells or tissues
} are
} washed well after glutaraldehyde fixation and before immersion in osmium
} tetroxide.
}
} The choice of buffers for EM is another subject that deserves a long
} discussion thread. Tom Phillips suggests the use of Good buffers as an
} alternative to cacodylate. However, PIPES and HEPES will preserve
} tissues
} and cells in very different ways when compared with each other, as well
} as
} with cacodylate or phosphate buffers. This is partially due to the
} amount of
} extraction that occurs. In addition, PIPES buffer is often used at a pH
} where it has almost no buffering capacity, HEPES can preserve tissues so
} well that there is almost no extraction of cellular material, which
} results
} in virtually no specimen contrast and TRIS buffer may not work at all
} due to
} its ability to react with the aldehyde. In the end, fixation recipe
} changes
} should be approached with some caution.
}
} Best regards,
}
} Paul.
}
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} (213) 273 8026
} pwebster-at-hei.org
}
}
} } From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
} } Date: Fri, 9 Jan 2009 15:18:37 -0600
} } To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com}
} } Subject: RE: [Microscopy] Cac buffer and undergrads - chancy?
} }
} } I naturally agree with Paul that with proper training that all the
} } chemicals used in an EM are safe but that doesn't make it a good idea
} to
} } use them indiscriminately. The comparison to bacteria and viruses is a
} } red herring since those are typically the subject of interest as
} opposed
} } to the selection of a buffer which is discretionary. Microscopists and
} } other scientists inevitably generate hazardous waste but it is
} incumbent
} } on us not to do so unless there is a scientific reason that it is the
} } only way to do the experiment.
} }
} } It is true that glutaraldehyde in phosphate buffer is dangerous. But
} } glutaraldehyde in cacodylate buffer is more dangerous. Accidental
} } exposure would mean exposure to two dangerous chemicals. In addition,
} } adding acid to glutaraldehyde in phosphate will change the pH but not
} } release arsenic gas. Many studies have shown the Good buffers to be
} } suitable for LM and EM studies. I see no reason for any microscopist
} to
} } cling to the use of cacodylate.
} }
} } Paul's own horror story of poor training and supervision is further
} } evidence of why one should minimize all unnecessary risks. I doubt any
} } trained scientist would any chemical they don't fully understand
} outside
} } of the hood and without gloves - that's a beginner's mistake. Unless
} one
} } can guarantee being present at every step of a new student's
} processing,
} } it would only be prudent to use the least toxic formulations. With
} } experience, those students will be able to judge the risks they wish
} to
} } take.
} }
} }
} } Thomas E. Phillips, Ph.D
} } Professor of Biological Sciences
} } Chair, MU Faculty Council
} } Director, Molecular Cytology Core
} } 2 Tucker Hall
} } University of Missouri
} } Columbia, MO 65211-7400
} } 573-882-4712 (office)
} } 573-882-0123 (fax)
} } phillipst-at-missouri.edu
} }
} } http://www.biology.missouri.edu/faculty/phillips.html
} } http://www.biotech.missouri.edu/mcc/
} }
} } -----Original Message-----
} } From: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
} } Sent: Friday, January 09, 2009 2:46 PM
} } To: Phillips, Thomas E.
} } Subject: [Microscopy] Cac buffer and undergrads - chancy?
} }
} }
} }
} }
} }
} ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ------------------------------------------------------------------------
} } ----
} }
} } Dear All,
} }
} } I have always found it strange that there is so much concern about the
} } use
} } of cacodylate in the EM laboratory. I concede that it is dangerous
} when
} } handled incorrectly. However, I would think that phosphate buffer
} } containing
} } 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution
} of
} } osmium tetroxide. At what point should students start taking
} } responsibility
} } for handling chemicals safely and when do the trainers bite the bullet
} } and
} } make sure the training is adequate?
} }
} } During my training I remember very clearly how I was taught how to
} } handle
} } pathogenic bacteria and viruses. The instructions were very clear and
} } very
} } strict, and followed the same basic rules of how I was trained to
} handle
} } radioactive material. Basically the instructions were that these
} agents
} } can
} } make you sick and even kill you if you don't follow my instructions,
} so
} } you
} } have to handle them as follows....
} }
} } When I moved into electron microscopy, the training I was given to
} } prepare
} } biological specimens consisted of being given access to a fridge full
} of
} } chemicals and a written protocol. The brown spots on my hands appeared
} } after
} } a couple of hours but my corneas clouded over in about 30 min of my
} } using
} } the osmium tetroxide. I had been left completely unsupervised to
} handle
} } these chemicals without any prior warning of their dangers.
} } Interestingly,
} } when my supervisor found out about my use of the osmium tetroxide, and
} } what
} } it had done to me, he blamed me!
} }
} } With proper training, the chemicals we use in the EM lab are basically
} } very
} } safe. We use small amounts of them and store them in closed cabinets
} so
} } they
} } should not affect our health in any way.
} }
} } Part of a good laboratory training course is teaching users how to
} } handle
} } toxic chemicals and how to pipette solutions without creating
} aerosols.
} } Making sure that protective gloves are used correctly is another
} } important
} } aspect of this training.
} }
} } If we train correctly, then it should be sufficient to warn students
} } that
} } they are handling materials designed to chemically alter biological
} } material. At this point, I usually remind them that they are made of
} } biological material too.
} }
} } Happy New Year
} } (My New Years Resolution is very high!)
} }
} } Best regards,
} }
} } Paul Webster.
} }
} } Paul Webster, Ph.D
} } House Ear Institute
} } 2100 West Third Street
} } Los Angeles, CA 90057
} } (213) 273 8026
} } pwebster-at-hei.org
} }
} }
} }
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009
} } 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org
} [12.88.48.54]
} } (may be forged))
} } 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n09KjYwS028110
} } 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009
} } 14:45:35 -0600
} } 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by
} } hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server
} HTTP-DAV
} } ;
} } 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000
} } 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119
} } 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800
} } 16, 18 -- Subject: Cac buffer and undergrads - chancy?
} } 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org}
} } 16, 18 -- To: {Microscopy-at-Microscopy.Com}
} } 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org}
} } 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy?
} } 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw==
} } 16, 18 -- Mime-version: 1.0
} } 16, 18 -- Content-type: text/plain;
} } 16, 18 -- charset="US-ASCII"
} } 16, 18 -- Content-transfer-encoding: 7bit
} } ==============================End of -
} } Headers==============================
} }
}


==============================Original Headers==============================
9, 19 -- From PWebster-at-hei.org Fri Jan 9 17:38:47 2009
9, 19 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09NckbP012473
9, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 17:38:47 -0600
9, 19 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ;
9, 19 -- Fri, 9 Jan 2009 23:38:46 +0000
9, 19 -- User-Agent: Microsoft-Entourage/12.15.0.081119
9, 19 -- Date: Fri, 09 Jan 2009 15:38:44 -0800
9, 19 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy?
9, 19 -- From: "Webster, Paul" {PWebster-at-hei.org}
9, 19 -- To: {Microscopy-at-microscopy.com}
9, 19 -- Message-ID: {C58D2004.1FB7C%PWebster-at-hei.org}
9, 19 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy?
9, 19 -- Thread-Index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQAASC0WwAACdVkAAAqzm5
9, 19 -- In-Reply-To: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD675-at-UM-XMAIL06.um.umsystem.edu}
9, 19 -- Mime-version: 1.0
9, 19 -- Content-type: text/plain;
9, 19 -- charset="US-ASCII"
9, 19 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Fri, 9 Jan 2009 18:07:34 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Bill,

Yes, I remember all this being discussed by a few of us, including you,
back a few years ago. One guy essentially called my certified Geiger
counter a liar. Like I saw in the past, I keep seeing web pages (EPA and
Health Canada, for example) that say that DU is still 0.2-0.4 % U-235
versus the original 0.7%. I always remember that it is roughly only half
to 70% depleted. My observation of the phenomena.

Someone strongly pointed out that U is an alpha emitter, not a gamma
emitter, and I was not reading gamma radiation (but I was). You pointed
out that it was the decay impurities that were the gamma emitters and that
was what I was reading on my counter. I agree that U-238 and DU are still
radioactive.

http://www.hc-sc.gc.ca/ewh-semt/pubs/radiation/uranium-eng.php
"It (uranium) consists of three isotopes: uranium-234, uranium-235 and
uranium-238 which are present in amounts of 0.005%, 0.7%, and 99.3%, by
weight, respectively."
"The amounts of uranium-234 and uranium-235 remaining in depleted uranium
metal are about 0.002% and 0.2%, respectively."
Here's my favorite. "depleted uranium is 40% less radioactive than natural
uranium." That means 60% of the radioactivity is still there. I am not
sure that includes gamma but probably.

My main point this time around was that 'whatever was in all my bottles of
DUAc', it was also a gamma emitter. Also, it is impossible to know the
history of the manufacturing of the material and so one should take their
own Geiger counter readings to see what gamma radiation is actually there.
That's the final referee on the raw salt, IMO. It's also a measure of the
impurities, I guess. Of course the dilution level makes the gamma levels
less in the final solutions used in EM.

JMO,

Paul

At 04:14 PM 1/9/09 -0600, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
8, 28 -- From beaurega-at-westol.com Fri Jan 9 18:07:34 2009
8, 28 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0A07XPt026675
8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 18:07:34 -0600
8, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
8, 28 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n0A07Ub9025500
8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 19:07:31 -0500
8, 28 -- Received: (qmail 10525 invoked by uid 89); 10 Jan 2009 00:07:22 -0000
8, 28 -- Received: from pitts-69-72-13-226.dynamic-dialup.coretel.net (HELO running) (69.72.13.226)
8, 28 -- by mail.winbeam.com with SMTP; 10 Jan 2009 00:07:22 -0000
8, 28 -- Message-Id: {3.0.6.32.20090109190806.007dca80-at-pop3.norton.antivirus}
8, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus
8, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
8, 28 -- Date: Fri, 09 Jan 2009 19:08:06 -0500
8, 28 -- To: tivol-at-caltech.edu, microscopy-at-microscopy.com
8, 28 -- From: Beaurega {beaurega-at-westol.com}
8, 28 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters
8, 28 -- In-Reply-To: {200901092214.n09MEsUf022098-at-ns.microscopy.com}
8, 28 -- Mime-Version: 1.0
8, 28 -- Content-Type: text/plain; charset="us-ascii"
8, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
8, 28 -- X-Winbeam-MailScanner-ID: n0A07Ub9025500
8, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
8, 28 -- X-Winbeam-MailScanner-SpamCheck: notspam (whitelisted), spamassassin (cached,
8, 28 -- score=-1.904, required 4, autolearn=not spam, AWL 0.10,
8, 28 -- BAYES_00 -2.00)
8, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
8, 28 -- X-Winbeam-MailScanner-Watermark: 1232150851.88685-at-le6fs513bArGrZgGqeFfmg
==============================End of - Headers==============================




From: tiger3g3-at-yahoo.com
Date: Fri, 9 Jan 2009 22:27:25 -0600
Subject: [Microscopy] RE: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One more consideration is the logistics of it all: are the students
early college and new to science or advanced and already well on a
science path? I say, save the hazardous stuff for the latter group,
generally. Let them get hooked before you trot out the dangers! ;-)
Also: class size matters a lot, and how much other stuff you have to
fit into the class session (hazardous stuff needs a bit of time).
I've found that most students will be fine, but there's always
someone who wasn't paying attention and isn't careful, so you need a
small class and a good lab setup (dangerous stuff not too close to
other stuff). In one class (intro level) we were doing blood draws
(for blood typing) and I was very surprised at how careless they were
(especially when they were distracted by results), so I'd call them
up in small groups, but this took a while and some of them still
managed to get blood where it shouldn't have been. (Nothing serious,
but it kept me on my toes a bit too much for comfort.)

In training my students in microscopy, I try to impart good habits,
figuring, better they should start at the "taking extreme care level"
then when their habits inevitably decay, they'll still have decent
enough habits. It's funny when I forget myself to follow the rules
(always koehler, no food near scopes, start on low power). Just the
other day my own tech glared at me when I walked into the scope room,
and it took me a sec, but i realized that i was holding (and eating)
very crumbly food.

On the other extreme, we once had a stockroom tech here who was very
afraid of all chemicals, despite her degree in biology. She told me
(I have this in email, so I wasn't hallucinating it) that she
couldn't prepare a NaCl solution for me because she hadn't had safety
training yet. Really!

Gisele



==============================Original Headers==============================
6, 27 -- From tiger3g3-at-yahoo.com Fri Jan 9 22:27:25 2009
6, 27 -- Received: from n17.bullet.mail.mud.yahoo.com (n17.bullet.mail.mud.yahoo.com [68.142.206.144])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0A4RPM1014323
6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:27:25 -0600
6, 27 -- Received: from [68.142.194.244] by n17.bullet.mail.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000
6, 27 -- Received: from [68.142.201.66] by t2.bullet.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000
6, 27 -- Received: from [127.0.0.1] by omp418.mail.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000
6, 27 -- X-Yahoo-Newman-Id: 144280.94259.bm-at-omp418.mail.mud.yahoo.com
6, 27 -- Received: (qmail 10754 invoked from network); 10 Jan 2009 04:27:24 -0000
6, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 27 -- s=s1024; d=yahoo.com;
6, 27 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:Mime-Version:X-Sender:Message-Id:In-Reply-To:References:Date:To:From:Subject:Content-Type;
6, 27 -- b=GASZ7cSffEmx323Qb16TrqtdbcP/SnEoR0vEf8//n0B03XAdsDlaKb1HXQ89y9cHb9XRhu8tpYLNUtp1AESktNO8NmwSNyzIkQGyZJGlo7D4TtB5Qk52/jh344F4Au7M1TuRWnIfVkFK2eN0/eNbBqeoXRvLbYubV5NZSV+cKn4= ;
6, 27 -- Received: from unknown (HELO ?98.207.93.115?) (tiger3g3-at-98.207.93.115 with plain)
6, 27 -- by smtp105.plus.mail.sp1.yahoo.com with SMTP; 10 Jan 2009 04:27:24 -0000
6, 27 -- X-YMail-OSG: e7IKwhcVM1l3xBi7aKLaR11lkO5AIcUMmtp5Ce6HYsv2vUvu50c1nRr4X.4sh_tcEIe_bIfMmsW_CxDFCpdpTGN0KwsWDLNEGGPChjfh476nlZsYepenCBKGQzGosezMyVCtpuoGQ8d5N9JFOL_rV.2XvycnRs91p4HPpEQGVrEt45Gi1T6yjLA4oZo-
6, 27 -- X-Yahoo-Newman-Property: ymail-3
6, 27 -- Mime-Version: 1.0
6, 27 -- X-Sender: tiger3g3-at-pop.mail.yahoo.com
6, 27 -- Message-Id: {a05111b44c58dd02ea52e-at-[98.207.93.115]}
6, 27 -- In-Reply-To: {200901092123.n09LNTVK021406-at-ns.microscopy.com}
6, 27 -- References: {200901092123.n09LNTVK021406-at-ns.microscopy.com}
6, 27 -- Date: Fri, 9 Jan 2009 20:27:23 -0800
6, 27 -- To: microscopy-at-microscopy.com
6, 27 -- From: Gisele Eliane Giorgi {tiger3g3-at-yahoo.com}
6, 27 -- Subject: [Microscopy] RE: Cac buffer and undergrads - chancy?
6, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: heckman-at-buckeye-express.com
Date: Sat, 10 Jan 2009 00:50:38 -0600
Subject: [Microscopy] polishing stainless steel and silver metal surfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers - I have samples to prepare for inspection by SEM, and the client wants to know about the grain size. We have never polished metals before. Is there anyone who can advise me what to do, or does this take a metallurgist? Carol Heckman, Bowling Green State University

==============================Original Headers==============================
1, 19 -- From heckman-at-buckeye-express.com Sat Jan 10 00:50:38 2009
1, 19 -- Received: from omta0106.mta.everyone.net (imta-38.everyone.net [216.200.145.38])
1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0A6ocXh001891
1, 19 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 00:50:38 -0600
1, 19 -- Received: from dm24.mta.everyone.net (sj1-slb03-gw2 [172.16.1.96])
1, 19 -- by omta0106.mta.everyone.net (Postfix) with ESMTP id 14B4072C3DD
1, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:51:05 -0800 (PST)
1, 19 -- X-Eon-Dm: dm24
1, 19 -- Received: by resin15.mta.everyone.net (EON-PICKUP)
1, 19 -- id resin15.4962afe0.2912b; Fri, 9 Jan 2009 22:50:37 -0800
1, 19 -- MIME-Version: 1.0
1, 19 -- Content-Type: text/plain; charset="UTF-8"
1, 19 -- Message-Id: {20090109225037.A97AB905-at-resin15.mta.everyone.net}
1, 19 -- Date: Fri, 9 Jan 2009 22:50:37 -0800
1, 19 -- From: {heckman-at-buckeye-express.com}
1, 19 -- To: {microscopy-at-microscopy.com}
1, 19 -- Subject: polishing stainless steel and silver metal surfaces
1, 19 -- X-Eon-Sig: AQK8SgtJaEU99JcqNgEAAAAB,8565da5615434e3c9ae0f09b36273f8f
1, 19 -- X-Originating-Ip: [72.241.42.208]
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Sat, 10 Jan 2009 07:13:49 -0600
Subject: [Microscopy] polishing stainless steel and silver metal surfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Carol,

You might be better off delegating this one to somebody in the materials
science department, but if you want to try it yourself, I can help you with
the silver. You didn't mention what silver alloy you're attempting to
prepare, or it's form, but here's a method that works well for preparing
fine and sterling silver, yellow and white gold alloys, nickel, copper
alloys, and silver solders on rotating wheels at 400 rpm. The type of
lubricant, cloth selection, and pressure are important.

1) Grind through a succession of SiC papers from 240 through 600 grit using
moderate pressure with running water lubricant. Rinse or ultrasonically
clean after each paper. Dry.
2) Coarse polish on a napless cotton cloth with 6 micron diamond (I use a
suspension), and moderate pressure with a commercial diamond extender.
Ultrasonically clean and dry.
3) Fine polish on a short-napped synthetic velvet cloth with 1 micron
diamond (I use a suspension) and deionized water lubricant using moderate to
light pressure. Ultrasonically clean and dry.

You'll have to etch the silver to reveal the grain size after polishing.
Without knowing what silver alloy you're working with, try a 50/50 mixture
of ammonium hydroxide and hydrogen peroxide (30% concentration). The etch
works very fast. It can be diluted with DI water to slow the attack. Apply
by swabbing. Do not store.

Good luck,

Jeff Stewart
Metallographic Lab Manager
Stern-Leach Company
Cookson Precious Metals
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329

-----Original Message-----
X-from: heckman-at-buckeye-express.com [mailto:heckman-at-buckeye-express.com]
Sent: Saturday, January 10, 2009 1:58 AM
To: jeff-at-metallography.com

Listers - I have samples to prepare for inspection by SEM, and the client
wants to know about the grain size. We have never polished metals before.
Is there anyone who can advise me what to do, or does this take a
metallurgist? Carol Heckman, Bowling Green State University

==============================Original Headers==============================
1, 19 -- From heckman-at-buckeye-express.com Sat Jan 10 00:50:38 2009
1, 19 -- Received: from omta0106.mta.everyone.net (imta-38.everyone.net
[216.200.145.38])
1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0A6ocXh001891
1, 19 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 00:50:38
-0600
1, 19 -- Received: from dm24.mta.everyone.net (sj1-slb03-gw2 [172.16.1.96])
1, 19 -- by omta0106.mta.everyone.net (Postfix) with ESMTP id
14B4072C3DD
1, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:51:05
-0800 (PST)
1, 19 -- X-Eon-Dm: dm24
1, 19 -- Received: by resin15.mta.everyone.net (EON-PICKUP)
1, 19 -- id resin15.4962afe0.2912b; Fri, 9 Jan 2009 22:50:37 -0800
1, 19 -- MIME-Version: 1.0
1, 19 -- Content-Type: text/plain; charset="UTF-8"
1, 19 -- Message-Id: {20090109225037.A97AB905-at-resin15.mta.everyone.net}
1, 19 -- Date: Fri, 9 Jan 2009 22:50:37 -0800
1, 19 -- From: {heckman-at-buckeye-express.com}
1, 19 -- To: {microscopy-at-microscopy.com}
1, 19 -- Subject: polishing stainless steel and silver metal surfaces
1, 19 -- X-Eon-Sig:
AQK8SgtJaEU99JcqNgEAAAAB,8565da5615434e3c9ae0f09b36273f8f
1, 19 -- X-Originating-Ip: [72.241.42.208]
==============================End of - Headers==============================
Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.176 / Virus Database: 270.9.13/1825 - Release Date: 12/2/2008
8:44 PM


==============================Original Headers==============================
12, 39 -- From SRS0=BqBZVq=5O=metallography.com=jeff-at-eigbox.net Sat Jan 10 07:13:48 2009
12, 39 -- Received: from bosmailout13.eigbox.net (bosmailout13.eigbox.net [66.96.190.13])
12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0ADDm4T030867
12, 39 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 07:13:48 -0600
12, 39 -- Message-Id: {200901101313.n0ADDm4T030867-at-ns.microscopy.com}
12, 39 -- Received: from bosmailscan16.eigbox.net ([10.20.15.16])
12, 39 -- by bosmailout13.eigbox.net with esmtp (Exim)
12, 39 -- id 1LLdeu-0005st-5e
12, 39 -- for microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:48 -0500
12, 39 -- Received: from bosimpout01.eigbox.net ([10.20.55.1])
12, 39 -- by bosmailscan16.eigbox.net with esmtp (Exim)
12, 39 -- id 1LLdeu-0001p7-3n
12, 39 -- for microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:48 -0500
12, 39 -- Received: from bosauthsmtp04.eigbox.net ([10.20.18.4])
12, 39 -- by bosimpout01.eigbox.net with NO UCE
12, 39 -- id 1kzR1b00205GATN0000000; Sat, 10 Jan 2009 03:59:25 -0500
12, 39 -- X-EN-OrigOutIP: 10.20.18.4
12, 39 -- X-EN-IMPSID: 1kzR1b00205GATN0000000
12, 39 -- Received: from pool-71-184-39-246.bstnma.east.verizon.net ([71.184.39.246] helo=Jeff)
12, 39 -- by bosauthsmtp04.eigbox.net with esmtpa (Exim)
12, 39 -- id 1LLdei-0004z3-GC
12, 39 -- for Microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:37 -0500
12, 39 -- From: "Jeff Stewart" {jeff-at-metallography.com}
12, 39 -- To: {Microscopy-at-microscopy.com}
12, 39 -- Subject: RE: [Microscopy] polishing stainless steel and silver metal surfaces
12, 39 -- Date: Sat, 10 Jan 2009 08:13:26 -0500
12, 39 -- MIME-Version: 1.0
12, 39 -- Content-Type: text/plain;
12, 39 -- charset="us-ascii"
12, 39 -- Content-Transfer-Encoding: 7bit
12, 39 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
12, 39 -- Thread-Index: Acly8LYf6PtvJgQ4TkOQ3DxLbWpvMgAL34gg
12, 39 -- In-Reply-To: {200901100657.n0A6vW0N015262-at-ns.microscopy.com}
12, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
12, 39 -- X-EN-UserInfo: e86f17d72db2b073c5072c937c9a2a3f:f6ee1f837e2a1583e399b28f5cf1cc41
12, 39 -- X-EN-AuthUser: jeff-at-metallography.com
12, 39 -- Sender: "Jeff Stewart" {jeff-at-metallography.com}
12, 39 -- X-EN-OrigIP: 71.184.39.246
12, 39 -- X-EN-OrigHost: pool-71-184-39-246.bstnma.east.verizon.net
==============================End of - Headers==============================




From: as-at-astonmet.com
Date: Sat, 10 Jan 2009 09:26:01 -0600
Subject: [Microscopy] Re: polishing stainless steel and silver metal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you are looking to measure the grain size of the stainless steel,
refer to ASTM E112 for the measurement procedure. You will need to
properly polish and etch first. The alloy will dictate the proper
etchant to use. Try Viella's for 400 series. For 300 series, try
Kalling's II, Glyceregia or electrolytic 10% oxalic.

Do not store glyceregia!

Alan Stone
ASTON


At 12:51 AM 1/10/2009, heckman-at-buckeye-express.com wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.

This confidential message is for the private use of the recipient as
shown. If this has been delivered to you in error, then please return
the message and delete from your files. Thank you.


==============================Original Headers==============================
12, 22 -- From as-at-astonmet.com Sat Jan 10 09:26:00 2009
12, 22 -- Received: from outbound2.mail.tds.net (outbound2.mail.tds.net [216.170.230.92])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AFQ07a015853
12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:26:00 -0600
12, 22 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32])
12, 22 -- by outbound2.mail.tds.net (8.13.6/8.13.4) with ESMTP id n0AFQ0PY000412
12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:26:00 -0600
12, 22 -- Received: from mozart.astonmet.com ([69.11.219.4])
12, 22 -- by outaamta02.mail.tds.net with ESMTP
12, 22 -- id {20090110152559.DTZC4416.outaamta02.mail.tds.net-at-mozart.astonmet.com}
12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:25:59 -0600
12, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 22 -- Date: Sat, 10 Jan 2009 09:25:58 -0600
12, 22 -- To: microscopy-at-microscopy.com
12, 22 -- From: Alan Stone {as-at-astonmet.com}
12, 22 -- Subject: Re: [Microscopy] polishing stainless steel and silver metal
12, 22 -- surfaces
12, 22 -- In-Reply-To: {200901100651.n0A6pis2003576-at-ns.microscopy.com}
12, 22 -- References: {200901100651.n0A6pis2003576-at-ns.microscopy.com}
12, 22 -- Mime-Version: 1.0
12, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
12, 22 -- Message-Id: {20090110152559.DTZC4416.outaamta02.mail.tds.net-at-mozart.astonmet.com}
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Sat, 10 Jan 2009 10:50:09 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I'm sorry about sounding a bit negative in my previous post. I did not
mean to school anybody.

I just wanted to clarify that the radioactive decay process of U-238
continues via further decay of daughter nuclei with different modes of
decay. And people can do their own calculations, as well.

Regards,
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================

On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Ayten,
}
} Your comments are informative but, to my personal taste, definitely a
} bit negative in tone for this list. Your "schooling" of Alex isn't
} really necessary and I think we could get your information in a more
} positive and collegial way.
}
} Regarding the decay tree, I note from the provided link information that
} the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
} my high school chemistry but I'm wondering how much Th-234 and
} associated beta emission danger we are really dealing with here; seems
} like there must be a very small amount of Th-234 produced with such a
} long half-life of the original U-238. Maybe you could comment on the
} danger of this.
}
} Thanks,
}
} Dale
}
}
} celikaktas-at-gmail.com wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear Alex,
} }
} } Did you have a chance to check the information from
} }
} } http://atom.kaeri.re.kr/ton/nuc7.html
} }
} } Let me construct the decay tree. I'm sure everybody in this list can
} } do it but, you keep insisting that "uranyl compounds are alpha
} } emitters only" so, I will take the time to do the job and post in to
} } the list.
} }
} } Let's start with U-238 which is the starting element in your compound.
} }
} } 1) U-238 decays into Th-234 by Alpha decay
} }
} } 2) Th-234 decays into Pa-234 by Beta decay
} }
} } 3) Pa-234 decays into U-234 by Beta decay
} }
} } 4) U-234 decays into Th-230 by Alpha decay
} }
} } 5) Th-230 decays into Ra-226 by Alpha decay
} }
} } 6) Ra-226 decays into Rn-222 by Alpha decay
} }
} } 7) Rn-222 decays into Po-218 by Alpha decay
} }
} } 8) Po-218 decays into Pb-214 by Alpha decay
} }
} } 9) Pb-214 decays into Bi-214 by Beta decay
} }
} } 10) Bi-214 decays into Po-214 by Beta decay
} }
} } 11) Po-214 decays into Pb-210 by Alpha decay
} }
} } 12) Pb-210 decays into Bi-210 by Beta decay
} }
} } 13) Bi-210 decays into Po-210 by Beta decay
} }
} } 14) Po-210 decays into Pb-206 by Alpha decay
} }
} } Pb-206 is STABLE so, it is the last element to be produced as a result
} } of U-238 radioactive decay.
} }
} } I have constructed the above decay tree using the information from
} } http://atom.kaeri.re.kr/ton/nuc7.html
} } While constructing the above decay tree I have used the branch which
} } has the highest branch ratio (above 99% in each case).
} }
} } I do not understand why you are trying to keep things "under control"?
} }
} } By the way, I'm a Nuclear Engineer.
} }
} } One does not even need to be nuclear engineer to understand this. Even
} } in high school science classes people learn about radioactive decay
} } series e.g. A decays into B and B decays into C...
} }
} } Best,
} } Ayten.
} }
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================

==============================Original Headers==============================
7, 34 -- From celikaktas-at-gmail.com Sat Jan 10 10:50:09 2009
7, 34 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.157])
7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AGo8B5031858
7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 10:50:09 -0600
7, 34 -- Received: by fg-out-1718.google.com with SMTP id 13so3751505fge.4
7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 08:50:08 -0800 (PST)
7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 34 -- d=gmail.com; s=gamma;
7, 34 -- h=domainkey-signature:mime-version:received:in-reply-to:references
7, 34 -- :date:message-id:subject:from:to:content-type
7, 34 -- :content-transfer-encoding;
7, 34 -- bh=b7SWPFZhMaWulbg1hGRPoUW6eYkvHtar8baNSsG43Z4=;
7, 34 -- b=m6xReT0PDvt+APSQ6xOAxowj5D8DRGNg/bb+JG79oNc2HYhEhuTh9MtHO4oxtZFgiJ
7, 34 -- 6gUlLRLqq6iO9QomoUY2ApzZvVy5ibE25mjMdYIw36ykLMNyGPxvd0geHyrIE/HcbJTw
7, 34 -- sy3ySqK30W1KHQSrpz3P2BccaH10kqzXJON3w=
7, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 34 -- d=gmail.com; s=gamma;
7, 34 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
7, 34 -- :content-type:content-transfer-encoding;
7, 34 -- b=x6wLqhBvlIB7ko+B5XBu53zczgEI647iuiv9yDkFq+Xm3ov7YKVjwqQ11peUA1lb9N
7, 34 -- k31yM6C+OV/4dQcHT4SGtgbvtbt2wRrDGp4CJpXUZ+sNM91L9SEMCvmkc7tIQJutoUYR
7, 34 -- BjkHZIK2Q5wsp3wzSv9YsQXZX0KASiwGb5WwE=
7, 34 -- MIME-Version: 1.0
7, 34 -- Received: by 10.103.217.5 with SMTP id u5mr9668029muq.42.1231606207534; Sat,
7, 34 -- 10 Jan 2009 08:50:07 -0800 (PST)
7, 34 -- In-Reply-To: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
7, 34 -- References: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
7, 34 -- Date: Sat, 10 Jan 2009 18:50:07 +0200
7, 34 -- Message-ID: {1075c5c10901100850u20dcb242nd050b7702a79e92d-at-mail.gmail.com}
7, 34 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters
7, 34 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
7, 34 -- To: microscopy {Microscopy-at-microscopy.com}
7, 34 -- Content-Type: text/plain; charset=UTF-8
7, 34 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Sat, 10 Jan 2009 14:35:06 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Based on Dale's reported half life for U238, the specific activity for U238
would be 1.2x10exp4 DPS, or about 0.3 uCi/g. This is very small activity
and they're alpha particles, which can be shielded best by plastic to avoid
Bremstrahlung and secondary x-ray production. The daughter products will
accumulate and decay according to their schemes and half-lives, which makes
it more difficult to calculate. (See "Nuclear and Radiochemistry", by
Friedlander, Kennedy, Miller, for a treatise on the differential equations
to calculate activity from multiple decay pathways.)

The best is to just measure it with a survey meter and see what the external
total dose rate in mr/hr is. Don't be afraid if your meter 'pegs' on the
lowest (most sensitive setting). A sample with a field over several mr/hr
over background should be handled according to practices used for handling
radioactive materials.

Hope that helps a bit.

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research

(Ex-Radiochemist)


Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com


-----Original Message-----
X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
Sent: Saturday, January 10, 2009 8:59 AM
To: dkloos-at-parallaxray.com

Dear Listers,

I'm sorry about sounding a bit negative in my previous post. I did not
mean to school anybody.

I just wanted to clarify that the radioactive decay process of U-238
continues via further decay of daughter nuclei with different modes of
decay. And people can do their own calculations, as well.

Regards,
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================

On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote:
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Dear Ayten,
}
} Your comments are informative but, to my personal taste, definitely a
} bit negative in tone for this list. Your "schooling" of Alex isn't
} really necessary and I think we could get your information in a more
} positive and collegial way.
}
} Regarding the decay tree, I note from the provided link information that
} the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
} my high school chemistry but I'm wondering how much Th-234 and
} associated beta emission danger we are really dealing with here; seems
} like there must be a very small amount of Th-234 produced with such a
} long half-life of the original U-238. Maybe you could comment on the
} danger of this.
}
} Thanks,
}
} Dale
}
}
} celikaktas-at-gmail.com wrote:
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } Dear Alex,
} }
} } Did you have a chance to check the information from
} }
} } http://atom.kaeri.re.kr/ton/nuc7.html
} }
} } Let me construct the decay tree. I'm sure everybody in this list can
} } do it but, you keep insisting that "uranyl compounds are alpha
} } emitters only" so, I will take the time to do the job and post in to
} } the list.
} }
} } Let's start with U-238 which is the starting element in your compound.
} }
} } 1) U-238 decays into Th-234 by Alpha decay
} }
} } 2) Th-234 decays into Pa-234 by Beta decay
} }
} } 3) Pa-234 decays into U-234 by Beta decay
} }
} } 4) U-234 decays into Th-230 by Alpha decay
} }
} } 5) Th-230 decays into Ra-226 by Alpha decay
} }
} } 6) Ra-226 decays into Rn-222 by Alpha decay
} }
} } 7) Rn-222 decays into Po-218 by Alpha decay
} }
} } 8) Po-218 decays into Pb-214 by Alpha decay
} }
} } 9) Pb-214 decays into Bi-214 by Beta decay
} }
} } 10) Bi-214 decays into Po-214 by Beta decay
} }
} } 11) Po-214 decays into Pb-210 by Alpha decay
} }
} } 12) Pb-210 decays into Bi-210 by Beta decay
} }
} } 13) Bi-210 decays into Po-210 by Beta decay
} }
} } 14) Po-210 decays into Pb-206 by Alpha decay
} }
} } Pb-206 is STABLE so, it is the last element to be produced as a result
} } of U-238 radioactive decay.
} }
} } I have constructed the above decay tree using the information from
} } http://atom.kaeri.re.kr/ton/nuc7.html
} } While constructing the above decay tree I have used the branch which
} } has the highest branch ratio (above 99% in each case).
} }
} } I do not understand why you are trying to keep things "under control"?
} }
} } By the way, I'm a Nuclear Engineer.
} }
} } One does not even need to be nuclear engineer to understand this. Even
} } in high school science classes people learn about radioactive decay
} } series e.g. A decays into B and B decays into C...
} }
} } Best,
} } Ayten.
} }
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================

==============================Original Headers==============================
7, 34 -- From celikaktas-at-gmail.com Sat Jan 10 10:50:09 2009
7, 34 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com
[72.14.220.157])
7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0AGo8B5031858
7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 10:50:09
-0600
7, 34 -- Received: by fg-out-1718.google.com with SMTP id 13so3751505fge.4
7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 08:50:08
-0800 (PST)
7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 34 -- d=gmail.com; s=gamma;
7, 34 --
h=domainkey-signature:mime-version:received:in-reply-to:references
7, 34 -- :date:message-id:subject:from:to:content-type
7, 34 -- :content-transfer-encoding;
7, 34 -- bh=b7SWPFZhMaWulbg1hGRPoUW6eYkvHtar8baNSsG43Z4=;
7, 34 --
b=m6xReT0PDvt+APSQ6xOAxowj5D8DRGNg/bb+JG79oNc2HYhEhuTh9MtHO4oxtZFgiJ
7, 34 --
6gUlLRLqq6iO9QomoUY2ApzZvVy5ibE25mjMdYIw36ykLMNyGPxvd0geHyrIE/HcbJTw
7, 34 -- sy3ySqK30W1KHQSrpz3P2BccaH10kqzXJON3w=
7, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 34 -- d=gmail.com; s=gamma;
7, 34 --
h=mime-version:in-reply-to:references:date:message-id:subject:from:to
7, 34 -- :content-type:content-transfer-encoding;
7, 34 --
b=x6wLqhBvlIB7ko+B5XBu53zczgEI647iuiv9yDkFq+Xm3ov7YKVjwqQ11peUA1lb9N
7, 34 --
k31yM6C+OV/4dQcHT4SGtgbvtbt2wRrDGp4CJpXUZ+sNM91L9SEMCvmkc7tIQJutoUYR
7, 34 -- BjkHZIK2Q5wsp3wzSv9YsQXZX0KASiwGb5WwE=
7, 34 -- MIME-Version: 1.0
7, 34 -- Received: by 10.103.217.5 with SMTP id
u5mr9668029muq.42.1231606207534; Sat,
7, 34 -- 10 Jan 2009 08:50:07 -0800 (PST)
7, 34 -- In-Reply-To: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
7, 34 -- References: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
7, 34 -- Date: Sat, 10 Jan 2009 18:50:07 +0200
7, 34 -- Message-ID:
{1075c5c10901100850u20dcb242nd050b7702a79e92d-at-mail.gmail.com}
7, 34 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha
emitters
7, 34 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
7, 34 -- To: microscopy {Microscopy-at-microscopy.com}
7, 34 -- Content-Type: text/plain; charset=UTF-8
7, 34 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================


==============================Original Headers==============================
18, 30 -- From dkloos-at-parallaxray.com Sat Jan 10 14:35:05 2009
18, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AKZ5fk021450
18, 30 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 14:35:05 -0600
18, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
18, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
18, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
18, 30 -- id 1LLkY3-0009W7-On; Sat, 10 Jan 2009 13:35:11 -0700
18, 30 -- Reply-To: {dkloos-at-parallaxray.com}
18, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
18, 30 -- To: {celikaktas-at-gmail.com}
18, 30 -- Cc: {microscopy-at-microscopy.com}
18, 30 -- References: {200901101659.n0AGx43H012719-at-ns.microscopy.com}
18, 30 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
18, 30 -- Date: Sat, 10 Jan 2009 12:34:58 -0800
18, 30 -- Organization: Parallax Research
18, 30 -- Message-ID: {E3F6FCD7F3614856949442747BC989B8-at-donl}
18, 30 -- MIME-Version: 1.0
18, 30 -- Content-Type: text/plain;
18, 30 -- charset="us-ascii"
18, 30 -- Content-Transfer-Encoding: 7bit
18, 30 -- X-Mailer: Microsoft Office Outlook 11
18, 30 -- Thread-Index: AclzRMI1owznMu3ZRCWFEukvLFTfPAAHKnng
18, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
18, 30 -- In-Reply-To: {200901101659.n0AGx43H012719-at-ns.microscopy.com}
18, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
18, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
18, 30 -- X-AntiAbuse: Original Domain - microscopy.com
18, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
18, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Sat, 10 Jan 2009 17:06:10 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I'm definitely not an expert in this field but from a bit of reading it
seems that it is anything but a clear and simple story and measurement
is clearly the way to go. In my poking around and reading I did run into
a mention of U-238 giving off gamma rays as a result of alpha emission.
Maybe this explains the gamma ray emission that Paul Beauregard
reported. For what it's worth.......

===============================================
http://en.wikipedia.org/wiki/Alpha_particles

Alpha particles (named after and denoted by the first letter in the
Greek alphabet, α) consist of two protons and two neutrons bound
together into a particle identical to a helium nucleus; hence, it can be
written as He2+ or 42He2+. They are a highly ionizing form of particle
radiation, and have low penetration.

Alpha particles are emitted by radioactive nuclei such as uranium,
thorium, actinium, or radium in a process known as alpha decay. This
sometimes leaves the nucleus in an excited state, with the emission of a
gamma ray removing the excess energy.

==================================================

Cheers!

Dale

dkloos-at-parallaxray.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Based on Dale's reported half life for U238, the specific activity for U238
} would be 1.2x10exp4 DPS, or about 0.3 uCi/g. This is very small activity
} and they're alpha particles, which can be shielded best by plastic to avoid
} Bremstrahlung and secondary x-ray production. The daughter products will
} accumulate and decay according to their schemes and half-lives, which makes
} it more difficult to calculate. (See "Nuclear and Radiochemistry", by
} Friedlander, Kennedy, Miller, for a treatise on the differential equations
} to calculate activity from multiple decay pathways.)
}
} The best is to just measure it with a survey meter and see what the external
} total dose rate in mr/hr is. Don't be afraid if your meter 'pegs' on the
} lowest (most sensitive setting). A sample with a field over several mr/hr
} over background should be handled according to practices used for handling
} radioactive materials.
}
} Hope that helps a bit.
}
} Don Kloos
} VP Sales, Marketing, Business Development
} Parallax Research
}
} (Ex-Radiochemist)
}
}
} Sales & Marketing
} 16478 Beach Blvd. #330
} Westminster, California, 92683-7860 USA
}
} TOLL FREE 1 866 581-XRAY (9729)
} Telephone 1 714 897-9779
} Fax 1 714 897-1421
} Email: dkloos-at-parallaxray.com
} SKYPE: don.kloos
} Website: http://www.parallaxray.com
}
}
} -----Original Message-----
} X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
} Sent: Saturday, January 10, 2009 8:59 AM
} To: dkloos-at-parallaxray.com
} Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I'm sorry about sounding a bit negative in my previous post. I did not
} mean to school anybody.
}
} I just wanted to clarify that the radioactive decay process of U-238
} continues via further decay of daughter nuclei with different modes of
} decay. And people can do their own calculations, as well.
}
} Regards,
} Ayten.
}
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
} On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote:
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} } Dear Ayten,
} }
} } Your comments are informative but, to my personal taste, definitely a
} } bit negative in tone for this list. Your "schooling" of Alex isn't
} } really necessary and I think we could get your information in a more
} } positive and collegial way.
} }
} } Regarding the decay tree, I note from the provided link information that
} } the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
} } my high school chemistry but I'm wondering how much Th-234 and
} } associated beta emission danger we are really dealing with here; seems
} } like there must be a very small amount of Th-234 produced with such a
} } long half-life of the original U-238. Maybe you could comment on the
} } danger of this.
} }
} } Thanks,
} }
} } Dale
} }
} }
} } celikaktas-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} ----------------------------------------------------------------------------
} } } Dear Alex,
} } }
} } } Did you have a chance to check the information from
} } }
} } } http://atom.kaeri.re.kr/ton/nuc7.html
} } }
} } } Let me construct the decay tree. I'm sure everybody in this list can
} } } do it but, you keep insisting that "uranyl compounds are alpha
} } } emitters only" so, I will take the time to do the job and post in to
} } } the list.
} } }
} } } Let's start with U-238 which is the starting element in your compound.
} } }
} } } 1) U-238 decays into Th-234 by Alpha decay
} } }
} } } 2) Th-234 decays into Pa-234 by Beta decay
} } }
} } } 3) Pa-234 decays into U-234 by Beta decay
} } }
} } } 4) U-234 decays into Th-230 by Alpha decay
} } }
} } } 5) Th-230 decays into Ra-226 by Alpha decay
} } }
} } } 6) Ra-226 decays into Rn-222 by Alpha decay
} } }
} } } 7) Rn-222 decays into Po-218 by Alpha decay
} } }
} } } 8) Po-218 decays into Pb-214 by Alpha decay
} } }
} } } 9) Pb-214 decays into Bi-214 by Beta decay
} } }
} } } 10) Bi-214 decays into Po-214 by Beta decay
} } }
} } } 11) Po-214 decays into Pb-210 by Alpha decay
} } }
} } } 12) Pb-210 decays into Bi-210 by Beta decay
} } }
} } } 13) Bi-210 decays into Po-210 by Beta decay
} } }
} } } 14) Po-210 decays into Pb-206 by Alpha decay
} } }
} } } Pb-206 is STABLE so, it is the last element to be produced as a result
} } } of U-238 radioactive decay.
} } }
} } } I have constructed the above decay tree using the information from
} } } http://atom.kaeri.re.kr/ton/nuc7.html
} } } While constructing the above decay tree I have used the branch which
} } } has the highest branch ratio (above 99% in each case).
} } }
} } } I do not understand why you are trying to keep things "under control"?
} } }
} } } By the way, I'm a Nuclear Engineer.
} } }
} } } One does not even need to be nuclear engineer to understand this. Even
} } } in high school science classes people learn about radioactive decay
} } } series e.g. A decays into B and B decays into C...
} } }
} } } Best,
} } } Ayten.
} } }
} } --
} } ===========================
} } Ayten Celik-Aktas, PhD
} } Ankara University
} } Electron Microscopy Laboratory
} } Ankara, Turkey
} } ===========================
}
} ==============================Original Headers==============================
} 7, 34 -- From celikaktas-at-gmail.com Sat Jan 10 10:50:09 2009
} 7, 34 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com
} [72.14.220.157])
} 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0AGo8B5031858
} 7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 10:50:09
} -0600
} 7, 34 -- Received: by fg-out-1718.google.com with SMTP id 13so3751505fge.4
} 7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 08:50:08
} -0800 (PST)
} 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 7, 34 -- d=gmail.com; s=gamma;
} 7, 34 --
} h=domainkey-signature:mime-version:received:in-reply-to:references
} 7, 34 -- :date:message-id:subject:from:to:content-type
} 7, 34 -- :content-transfer-encoding;
} 7, 34 -- bh=b7SWPFZhMaWulbg1hGRPoUW6eYkvHtar8baNSsG43Z4=;
} 7, 34 --
} b=m6xReT0PDvt+APSQ6xOAxowj5D8DRGNg/bb+JG79oNc2HYhEhuTh9MtHO4oxtZFgiJ
} 7, 34 --
} 6gUlLRLqq6iO9QomoUY2ApzZvVy5ibE25mjMdYIw36ykLMNyGPxvd0geHyrIE/HcbJTw
} 7, 34 -- sy3ySqK30W1KHQSrpz3P2BccaH10kqzXJON3w=
} 7, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 7, 34 -- d=gmail.com; s=gamma;
} 7, 34 --
} h=mime-version:in-reply-to:references:date:message-id:subject:from:to
} 7, 34 -- :content-type:content-transfer-encoding;
} 7, 34 --
} b=x6wLqhBvlIB7ko+B5XBu53zczgEI647iuiv9yDkFq+Xm3ov7YKVjwqQ11peUA1lb9N
} 7, 34 --
} k31yM6C+OV/4dQcHT4SGtgbvtbt2wRrDGp4CJpXUZ+sNM91L9SEMCvmkc7tIQJutoUYR
} 7, 34 -- BjkHZIK2Q5wsp3wzSv9YsQXZX0KASiwGb5WwE=
} 7, 34 -- MIME-Version: 1.0
} 7, 34 -- Received: by 10.103.217.5 with SMTP id
} u5mr9668029muq.42.1231606207534; Sat,
} 7, 34 -- 10 Jan 2009 08:50:07 -0800 (PST)
} 7, 34 -- In-Reply-To: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
} 7, 34 -- References: {200901091444.n09Ei6HG011125-at-ns.microscopy.com}
} 7, 34 -- Date: Sat, 10 Jan 2009 18:50:07 +0200
} 7, 34 -- Message-ID:
} {1075c5c10901100850u20dcb242nd050b7702a79e92d-at-mail.gmail.com}
} 7, 34 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha
} emitters
} 7, 34 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
} 7, 34 -- To: microscopy {Microscopy-at-microscopy.com}
} 7, 34 -- Content-Type: text/plain; charset=UTF-8
} 7, 34 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 18, 30 -- From dkloos-at-parallaxray.com Sat Jan 10 14:35:05 2009
} 18, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
} 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AKZ5fk021450
} 18, 30 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 14:35:05 -0600
} 18, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
} 18, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
} 18, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
} 18, 30 -- id 1LLkY3-0009W7-On; Sat, 10 Jan 2009 13:35:11 -0700
} 18, 30 -- Reply-To: {dkloos-at-parallaxray.com}
} 18, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
} 18, 30 -- To: {celikaktas-at-gmail.com}
} 18, 30 -- Cc: {microscopy-at-microscopy.com}
} 18, 30 -- References: {200901101659.n0AGx43H012719-at-ns.microscopy.com}
} 18, 30 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
} 18, 30 -- Date: Sat, 10 Jan 2009 12:34:58 -0800
} 18, 30 -- Organization: Parallax Research
} 18, 30 -- Message-ID: {E3F6FCD7F3614856949442747BC989B8-at-donl}
} 18, 30 -- MIME-Version: 1.0
} 18, 30 -- Content-Type: text/plain;
} 18, 30 -- charset="us-ascii"
} 18, 30 -- Content-Transfer-Encoding: 7bit
} 18, 30 -- X-Mailer: Microsoft Office Outlook 11
} 18, 30 -- Thread-Index: AclzRMI1owznMu3ZRCWFEukvLFTfPAAHKnng
} 18, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 18, 30 -- In-Reply-To: {200901101659.n0AGx43H012719-at-ns.microscopy.com}
} 18, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
} 18, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
} 18, 30 -- X-AntiAbuse: Original Domain - microscopy.com
} 18, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
} 18, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
} ==============================End of - Headers==============================

==============================Original Headers==============================
9, 20 -- From dac-at-research.umass.edu Sat Jan 10 17:06:10 2009
9, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AN69aV006061
9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 17:06:09 -0600
9, 20 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged))
9, 20 -- (authenticated bits=0)
9, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0AN68eV026533
9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 18:06:08 -0500
9, 20 -- Message-ID: {49692A12.7080904-at-research.umass.edu}
9, 20 -- Date: Sat, 10 Jan 2009 18:06:58 -0500
9, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
9, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
9, 20 -- MIME-Version: 1.0
9, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters
9, 20 -- References: {200901102040.n0AKekds029574-at-ns.microscopy.com}
9, 20 -- In-Reply-To: {200901102040.n0AKekds029574-at-ns.microscopy.com}
9, 20 -- Content-Type: text/plain; charset=UTF-8; format=flowed
9, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: przybylowicz-at-tlabs.ac.za
Date: Mon, 12 Jan 2009 08:10:52 -0600
Subject: [Microscopy] viaWWW: Post-doctoral fellow (NMP) - two years contract with

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both przybylowicz-at-tlabs.ac.za as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: przybylowicz-at-tlabs.ac.za
Name: Wojciech Przybylowicz

Organization: iThemba LABS, South Africa

Title-Subject: [Filtered] Post-doctoral fellow (NMP) - two years
contract with extension possibility

Question: Post-doctoral fellow (NMP) - two years contract with
extension possibility

Position: Nuclear microprobe analysis of thin frozen-hydrated
biological specimens

Applications are invited for this position in our Materials Research
Group (MRG) of iThemba LABS, situated ca. 30 km from Cape Town, South
Africa.

Responsibilities will include:
- Adapting the cryo-stage coupled to the MRG nuclear microprobe
for measurements of thin specimens in frozen-hydrated state
- Active involvement in research projects related to biological
applications of ion beam techniques and generating new applications.

Minimum requirements:

- PhD in Biology/Chemistry/Physics with strong emphasis on
cryo-preparation of biological specimens and low temperature electron
microscopy
- Experience in operating SEM/TEM and knowledge of EDS
technique as well as cryo-ultramicrotomy
- Experience in operating a nuclear microprobe and familiarity
with PIXE as an advantage
- Relevant conference presentations and publications as well as
international exposure
- Knowledge of statistical methods
- Computer literacy (Word, Excel, Corel, etc.)

We offer a competitive remuneration package, which includes normal
company benefits.

Forward your detailed CV, accompanied by a covering letter and
supporting documents, to the Human Resource Department; iThemba LABS,
P.O. Box 722, Somerset West 7129, or fax (+27-21-8433756), or via
e-mail to:
mirencia-at-tlabs.ac.za with copy to przybylowicz-at-tlabs.ac.za
For some information of the laboratory, visit our website: www.tlabs.ac.za

I suggest that you contact me for any details of this position.
E-mail: przybylowicz-at-tlabs.ac.za
You may want to read the following publication on earlier work done
by our team in this direction:

Grzegorz Tylko, Jolanta Mesjasz-Przybyłowicz and Wojciech J.
Przybyłowicz. X-ray microanalysis of biological material in the
frozen-hydrated state by PIXE. Microscopy Research and Technique
(2007) 70: 55-68.

Applications close on 28 February 2009


Login Host: 196.21.126.135
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 12 -- From zaluzec-at-microscopy.com Mon Jan 12 08:10:48 2009
17, 12 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
17, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CEAlQ2006692
17, 12 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 08:10:47 -0600
17, 12 -- Mime-Version: 1.0
17, 12 -- Message-Id: {p06240800c590ffbd9427-at-[206.69.208.22]}
17, 12 -- Date: Mon, 12 Jan 2009 08:10:45 -0600
17, 12 -- To: microscopy-at-microscopy.com
17, 12 -- From: przybylowicz-at-tlabs.ac.za (by way of MicroscopyListserver)
17, 12 -- Subject: viaWWW: Post-doctoral fellow (NMP) - two years contract with
17, 12 -- extension possibility
17, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tbargar-at-unmc.edu
Date: Mon, 12 Jan 2009 10:58:03 -0600
Subject: [Microscopy] TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

I have a TEM project, where the researcher wants to observe their monolayer
cell culture and the calcium crystals produced by those cells. The cells
produce extracellular calcium crystals which dissolve readily in water.
The usual fixation, post-fix, dehydration steps won't work . This situation
is unlike anything I have dealt with before, including all my years of
working with marine invertebrates. I am speculating that cryo methods may
be the only answer. As always thanks in advance for any help possible.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


==============================Original Headers==============================
5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009
5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CGw3WY025453
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600
5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600 (CST)
5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35])
5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600 (CST)
5, 20 -- Subject: TEM:cell culture with extracellular calcium
5, 20 -- To: Microscopy-at-microscopy.com
5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006
5, 20 -- Message-ID: {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu}
5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600
5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09
5, 20 -- AM
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Mon, 12 Jan 2009 11:06:12 -0600
Subject: [Microscopy] TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The obvious question would seem to be "How do the extracellular calcium
crystals survive in aqueous tissue culture medium?" Are you sure they
are dissolving or simply being washed away.

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Monday, January 12, 2009 10:59 AM
To: Phillips, Thomas E.


Listers,

I have a TEM project, where the researcher wants to observe their
monolayer
cell culture and the calcium crystals produced by those cells. The cells
produce extracellular calcium crystals which dissolve readily in water.
The usual fixation, post-fix, dehydration steps won't work . This
situation
is unlike anything I have dealt with before, including all my years of
working with marine invertebrates. I am speculating that cryo methods
may
be the only answer. As always thanks in advance for any help possible.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


==============================Original
Headers==============================
5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009
5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu
[192.198.54.127])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0CGw3WY025453
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:03 -0600
5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id
C8AFDA0076
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:02 -0600 (CST)
5, 20 -- Received: from unmcnotes01.unmc.edu
(host-137-197-103-35.unmc.edu [137.197.103.35])
5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id
A012539804F
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:00 -0600 (CST)
5, 20 -- Subject: TEM:cell culture with extracellular calcium
5, 20 -- To: Microscopy-at-microscopy.com
5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006
5, 20 -- Message-ID:
{OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu}
5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600
5, 20 -- X-MIMETrack: Serialize by Router on
UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09
5, 20 -- AM
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-type: text/plain; charset=US-ASCII
==============================End of -
Headers==============================


==============================Original Headers==============================
14, 29 -- From PhillipsT-at-missouri.edu Mon Jan 12 11:06:11 2009
14, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CH6BPt006584
14, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:06:11 -0600
14, 29 -- X-IronPort-Anti-Spam-Filtered: true
14, 29 -- X-IronPort-Anti-Spam-Result: ApoEAM8Ga0nRauUp/2dsb2JhbADITQ0BCYUihUsBhD+BLw
14, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
14, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 12 Jan 2009 11:06:11 -0600
14, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 29 -- Mon, 12 Jan 2009 11:06:11 -0600
14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 29 -- Content-class: urn:content-classes:message
14, 29 -- MIME-Version: 1.0
14, 29 -- Content-Type: text/plain;
14, 29 -- charset="us-ascii"
14, 29 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium
14, 29 -- Date: Mon, 12 Jan 2009 11:06:10 -0600
14, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD766-at-UM-XMAIL06.um.umsystem.edu}
14, 29 -- In-Reply-To: {200901121659.n0CGx0wY026584-at-ns.microscopy.com}
14, 29 -- X-MS-Has-Attach:
14, 29 -- X-MS-TNEF-Correlator:
14, 29 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium
14, 29 -- thread-index: Acl01xIfWxQoeHbdTM+DDiefgUiB8QAANJ1w
14, 29 -- References: {200901121659.n0CGx0wY026584-at-ns.microscopy.com}
14, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
14, 29 -- To: {Microscopy-at-microscopy.com}
14, 29 -- X-OriginalArrivalTime: 12 Jan 2009 17:06:11.0130 (UTC) FILETIME=[117359A0:01C974D8]
14, 29 -- Content-Transfer-Encoding: 8bit
14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CH6BPt006584
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Mon, 12 Jan 2009 11:20:46 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote:

} Someone strongly pointed out that U is an alpha emitter, not a gamma
} emitter, and I was not reading gamma radiation (but I was). You
} pointed
} out that it was the decay impurities that were the gamma emitters
} and that
} was what I was reading on my counter. I agree that U-238 and DU are
} still
} radioactive.

} Here's my favorite. "depleted uranium is 40% less radioactive than
} natural
} uranium." That means 60% of the radioactivity is still there. I am
} not
} sure that includes gamma but probably.


Dear Paul,
Since the activities of the U isotopes are inversely proportional to
their half-lives, and since the half lives of U235 and U234 are about
7 and 20,000 times shorter respectively than U238's, the amount of
radiation from U234 is about equal to that from U238, and that from
U235 is about 5% of that from the others, so your quote that DU has
only 60% the activity of natural U checks out. I pointed out that
~1/4 of the U238 decays are to an excited state of Th234, which is ~50
keV above the ground state, so pure U238 will produce some low-energy
gammas, as will many of the daughters. The bottom line is that direct
measurements performed correctly don't lie, so they are the best way
to settle this issue once and for all.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
7, 22 -- From tivol-at-caltech.edu Mon Jan 12 11:20:46 2009
7, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHKj7C020231
7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:20:46 -0600
7, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
7, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 95B5766E1E4F
7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 09:20:45 -0800 (PST)
7, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
7, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
7, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 7E59866E2F4D
7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 09:20:44 -0800 (PST)
7, 22 -- Message-Id: {C463CBB1-CCFF-43DE-A64B-E5D1522D3FA8-at-caltech.edu}
7, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- In-Reply-To: {200901100007.n0A07fpE026798-at-ns.microscopy.com}
7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
7, 22 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters
7, 22 -- Date: Mon, 12 Jan 2009 09:20:42 -0800
7, 22 -- References: {200901100007.n0A07fpE026798-at-ns.microscopy.com}
7, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: k.sader-at-leeds.ac.uk
Date: Mon, 12 Jan 2009 11:56:49 -0600
Subject: [Microscopy] Nanoplast resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have been searching around for some time and I have not been able to find
a supplier with any Nanoplast (melamine) resin. Does anyone know what
company actually produced Nanoplast, if they are still operating, or if
there is a supplier who still has some?

Thanks,

Kasim Sader



----------------------
Dr Kasim Sader
SuperSTEM
Daresbury Laboratory
Warrington
WA4 4AD




==============================Original Headers==============================
10, 30 -- From K.Sader-at-leeds.ac.uk Mon Jan 12 11:56:48 2009
10, 30 -- Received: from mhost03c.leeds.ac.uk (mhost03c.leeds.ac.uk [129.11.76.167])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHulM7002354
10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 11:56:48 -0600
10, 30 -- Received: from APOLLO1.ds.leeds.ac.uk (apollo1.leeds.ac.uk [129.11.5.4])
10, 30 -- by mhost03c.leeds.ac.uk (8.14.3/8.14.3) with ESMTP id n0CHukS2019389
10, 30 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT)
10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 17:56:46 GMT
10, 30 -- Received: from dexbr1.ds.leeds.ac.uk (129.11.76.19) by APOLLO1.ds.leeds.ac.uk
10, 30 -- (129.11.5.4) with Microsoft SMTP Server id 8.1.311.2; Mon, 12 Jan 2009
10, 30 -- 17:56:45 +0000
10, 30 -- Received: from HERMES4.ds.leeds.ac.uk ([129.11.5.130]) by
10, 30 -- dexbr1.ds.leeds.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 Jan
10, 30 -- 2009 17:56:45 +0000
10, 30 -- Received: from 129.11.40.48 ([129.11.40.48]) by HERMES4.ds.leeds.ac.uk
10, 30 -- ([129.11.5.131]) via Exchange Front-End Server outlook.leeds.ac.uk
10, 30 -- ([129.11.5.3]) with Microsoft Exchange Server HTTP-DAV ; Mon, 12 Jan 2009
10, 30 -- 17:56:45 +0000
10, 30 -- User-Agent: Microsoft-Entourage/11.4.0.080122
10, 30 -- Date: Mon, 12 Jan 2009 17:56:44 +0000
10, 30 -- Subject: Nanoplast resin
10, 30 -- From: Kasim Sader {k.sader-at-leeds.ac.uk}
10, 30 -- To: {Microscopy-at-Microscopy.Com}
10, 30 -- Message-ID: {C59134DC.619C%k.sader-at-leeds.ac.uk}
10, 30 -- Thread-Topic: Nanoplast resin
10, 30 -- Thread-Index: Acl03yEuX9NSRODSEd218gAZ4zduOA==
10, 30 -- MIME-Version: 1.0
10, 30 -- Content-Type: text/plain; charset="US-ASCII"
10, 30 -- Content-Transfer-Encoding: 7bit
10, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:56:45.0245 (UTC) FILETIME=[21EC8ED0:01C974DF]
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Mon, 12 Jan 2009 11:57:58 -0600
Subject: [Microscopy] Re: TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

Ca products will either dissolve or wash away, but will disappear from the
external and internal areas of the cells during aqueous sample
preparation.You really can't avoid this happening. The only way to minimize
the amount of dissolving (but not necessarily washing away if external) of
the Ca product is using HPF and FS.

I say this based on personal experience with internal calcium carbonate
inclusions in cells.

Debby

--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: {tbargar-at-unmc.edu}
} Reply-To: {tbargar-at-unmc.edu}
} Date: Mon, 12 Jan 2009 11:00:03 -0600
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] TEM:cell culture with extracellular calcium
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Listers,
}
} I have a TEM project, where the researcher wants to observe their monolayer
} cell culture and the calcium crystals produced by those cells. The cells
} produce extracellular calcium crystals which dissolve readily in water.
} The usual fixation, post-fix, dehydration steps won't work . This situation
} is unlike anything I have dealt with before, including all my years of
} working with marine invertebrates. I am speculating that cryo methods may
} be the only answer. As always thanks in advance for any help possible.
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}
} ==============================Original Headers==============================
} 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009
} 5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127])
} 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0CGw3WY025453
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600
} 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
} 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600
} (CST)
} 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu
} [137.197.103.35])
} 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600
} (CST)
} 5, 20 -- Subject: TEM:cell culture with extracellular calcium
} 5, 20 -- To: Microscopy-at-microscopy.com
} 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006
} 5, 20 -- Message-ID:
} {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu}
} 5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600
} 5, 20 -- X-MIMETrack: Serialize by Router on
} UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09
} 5, 20 -- AM
} 5, 20 -- MIME-Version: 1.0
} 5, 20 -- Content-type: text/plain; charset=US-ASCII
} ==============================End of - Headers==============================


==============================Original Headers==============================
8, 30 -- From dsherman-at-purdue.edu Mon Jan 12 11:57:57 2009
8, 30 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131])
8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHvvYQ004818
8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:57:57 -0600
8, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
8, 30 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n0CHvvA5020838
8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:57:57 -0500
8, 30 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11])
8, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n0CHvvne000488
8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:57:57 -0500
8, 30 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 30 -- Mon, 12 Jan 2009 12:57:56 -0500
8, 30 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ;
8, 30 -- Mon, 12 Jan 2009 17:57:08 +0000
8, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119
8, 30 -- Date: Mon, 12 Jan 2009 12:57:07 -0500
8, 30 -- Subject: Re: [Microscopy] TEM:cell culture with extracellular calcium
8, 30 -- From: Debby Sherman {dsherman-at-purdue.edu}
8, 30 -- To: {tbargar-at-unmc.edu} , "message to: MSA list" {microscopy-at-microscopy.com}
8, 30 -- Message-ID: {C590EEA3.38C43%dsherman-at-exchange.purdue.edu}
8, 30 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium
8, 30 -- Thread-Index: Acl03y7ktOL8Rky8Tk6hZdzF0JB74Q==
8, 30 -- In-Reply-To: {200901121700.n0CH03iD028637-at-ns.microscopy.com}
8, 30 -- Mime-version: 1.0
8, 30 -- Content-type: text/plain;
8, 30 -- charset="US-ASCII"
8, 30 -- Content-transfer-encoding: 7bit
8, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:57:56.0527 (UTC) FILETIME=[4C6953F0:01C974DF]
8, 30 -- X-PMX-Version: 5.4.0.320885
8, 30 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 12 Jan 2009 12:52:48 -0600
Subject: [Microscopy] TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

It's a long shot, but there are non-aqueous fixation techniques that
were originally developed to preserve biofilms that are usually removed
during conventional processing methods. These use a perfluorocarbon
solvent, such as the 3M company's FC-72, with osmium tetroxide to do the
fixation. If the calcium crystals will survive ethanol dehydration, it
may work. If not, it may be possible to experiment with ways of getting
the cells into resin directly from the solvent. Depends on how much
fuss you want to go through on a long-shot protocol!

One reference is: "Heterogeneity of the Composition and Thickness of
Tracheal Mucus in Rats", 1997, David Sims and Margaret Horne, Am J
Phys--Lung Cell & Mol Phsiol 17:L1036-L1041.

I can come up with more if you want to pursue this. 3M used to sell
this solvent in small quantities, although it's normally sold in drums
for electrical transformers, etc. Don't know if they still have the
smaller units.

For what it's worth...

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com








-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Monday, January 12, 2009 10:59 AM
To: Tindall, Randy D.


Listers,

I have a TEM project, where the researcher wants to observe their
monolayer
cell culture and the calcium crystals produced by those cells. The cells
produce extracellular calcium crystals which dissolve readily in water.
The usual fixation, post-fix, dehydration steps won't work . This
situation
is unlike anything I have dealt with before, including all my years of
working with marine invertebrates. I am speculating that cryo methods
may
be the only answer. As always thanks in advance for any help possible.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


==============================Original
Headers==============================
5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009
5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu
[192.198.54.127])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0CGw3WY025453
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:03 -0600
5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id
C8AFDA0076
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:02 -0600 (CST)
5, 20 -- Received: from unmcnotes01.unmc.edu
(host-137-197-103-35.unmc.edu [137.197.103.35])
5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id
A012539804F
5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
10:58:00 -0600 (CST)
5, 20 -- Subject: TEM:cell culture with extracellular calcium
5, 20 -- To: Microscopy-at-microscopy.com
5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006
5, 20 -- Message-ID:
{OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu}
5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600
5, 20 -- X-MIMETrack: Serialize by Router on
UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09
5, 20 -- AM
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-type: text/plain; charset=US-ASCII
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 30 -- From TindallR-at-missouri.edu Mon Jan 12 12:52:48 2009
24, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CIqmZF030867
24, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:52:48 -0600
24, 30 -- X-IronPort-Anti-Spam-Filtered: true
24, 30 -- X-IronPort-Anti-Spam-Result: ApoEANMga0nRauUo/2dsb2JhbADIdg0BCYU9hXCEQIEvhDI
24, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
24, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 12 Jan 2009 12:52:45 -0600
24, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
24, 30 -- Mon, 12 Jan 2009 12:52:45 -0600
24, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 30 -- Content-class: urn:content-classes:message
24, 30 -- MIME-Version: 1.0
24, 30 -- Content-Type: text/plain;
24, 30 -- charset="us-ascii"
24, 30 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium
24, 30 -- Date: Mon, 12 Jan 2009 12:52:45 -0600
24, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7CE0-at-UM-XMAIL08.um.umsystem.edu}
24, 30 -- In-Reply-To: {200901121659.n0CGxESU027070-at-ns.microscopy.com}
24, 30 -- X-MS-Has-Attach:
24, 30 -- X-MS-TNEF-Correlator:
24, 30 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium
24, 30 -- thread-index: Acl01xlVhTneargmT7eRSno8qDklvwADa9Ug
24, 30 -- References: {200901121659.n0CGxESU027070-at-ns.microscopy.com}
24, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
24, 30 -- To: {tbargar-at-unmc.edu}
24, 30 -- Cc: {microscopy-at-microscopy.com}
24, 30 -- X-OriginalArrivalTime: 12 Jan 2009 18:52:45.0431 (UTC) FILETIME=[F4C04070:01C974E6]
24, 30 -- Content-Transfer-Encoding: 8bit
24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CIqmZF030867
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Mon, 12 Jan 2009 13:11:29 -0600
Subject: [Microscopy] Re: TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds like rapid freezing followed by freeze substitution is
probably the way to go (as Debby Sherman suggested).

If you do not have access to such equipment, another possibility
would be to grow the cells directly on a TEM grid (with a Formvar
film, of course). Cu grids may be toxic to some cells but Ni should
be OK. We've grown cells on grids in the past and it works well once
you work out the concentration of cells (so as not to obliterate the
view).

In our situation, we rinsed to remove culture fluids but you could
plunge freeze the grid and freeze dry it. We made a freeze dryer (of
sorts) by having a copper block machined to accommodate specimens. It
had a lid (to prevent condensation). We loaded the specimens into the
LN2-chilled block and then put it in a vacuum evaporator. In your
case, 8 hr should dry the grid.

JB

} I have a TEM project, where the researcher wants to observe their monolayer
} cell culture and the calcium crystals produced by those cells. The cells
} produce extracellular calcium crystals which dissolve readily in water.
} The usual fixation, post-fix, dehydration steps won't work . This situation
} is unlike anything I have dealt with before, including all my years of
} working with marine invertebrates. I am speculating that cryo methods may
} be the only answer. As always thanks in advance for any help possible.
}
} Tom Bargar
} University of Nebraska Medical Center
}

--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Mon Jan 12 13:11:28 2009
8, 19 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CJBSV3012375
8, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:11:28 -0600
8, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
8, 19 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n0CJBRji028775
8, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:11:28 -0600 (CST)
8, 19 -- Mime-Version: 1.0
8, 19 -- Message-Id: {a06240801c59143799b8c-at-[131.230.177.136]}
8, 19 -- In-Reply-To: {200901121659.n0CGx6Eh026819-at-ns.microscopy.com}
8, 19 -- References: {200901121659.n0CGx6Eh026819-at-ns.microscopy.com}
8, 19 -- Date: Mon, 12 Jan 2009 13:11:24 -0600
8, 19 -- To: Microscopy-at-microscopy.com
8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 19 -- Subject: Re: [Microscopy] TEM:cell culture with extracellular calcium
8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 19 -- X-Spam-Score: 0.00%
8, 19 -- X-MASF: 0.00%
8, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: cervantes-at-bendres.com
Date: Mon, 12 Jan 2009 13:35:22 -0600
Subject: [Microscopy] TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom -
Just FYI: I used the non-aqueous method mentioned by Randy several years
ago, and it worked well for preserving the mucous layer on murine
intestinal tissue. The reference he suggested is a good start. Also,
if you call 3M, they may send you a sample bottle of Fluorinert FC-72,
which may be enough for your needs (and was enough for me to do a trial
run to ensure that the fixation worked).

Jessica

Jessica Cervantes
Bend Research, Inc
Bend, OR


Disclaimer: views expressed in this email are that of the sender and are
not necessarily reflective of Bend Research. This email is intended for
the addressee(s) only, and unauthorized access is prohibited.
____________________



------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

Tom,

It's a long shot, but there are non-aqueous fixation techniques that
were originally developed to preserve biofilms that are usually removed
during conventional processing methods. These use a perfluorocarbon
solvent, such as the 3M company's FC-72, with osmium tetroxide to do the
fixation. If the calcium crystals will survive ethanol dehydration, it
may work. If not, it may be possible to experiment with ways of getting
the cells into resin directly from the solvent. Depends on how much
fuss you want to go through on a long-shot protocol!

One reference is: "Heterogeneity of the Composition and Thickness of
Tracheal Mucus in Rats", 1997, David Sims and Margaret Horne, Am J
Phys--Lung Cell & Mol Phsiol 17:L1036-L1041.

I can come up with more if you want to pursue this. 3M used to sell
this solvent in small quantities, although it's normally sold in drums
for electrical transformers, etc. Don't know if they still have the
smaller units.

For what it's worth...

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com








-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Monday, January 12, 2009 10:59 AM
To: Tindall, Randy D.


Listers,

I have a TEM project, where the researcher wants to observe their
monolayer
cell culture and the calcium crystals produced by those cells. The cells
produce extracellular calcium crystals which dissolve readily in water.
The usual fixation, post-fix, dehydration steps won't work . This
situation
is unlike anything I have dealt with before, including all my years of
working with marine invertebrates. I am speculating that cryo methods
may
be the only answer. As always thanks in advance for any help possible.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu



==============================Original Headers==============================
32, 22 -- From cervantes-at-bendres.com Mon Jan 12 13:35:22 2009
32, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113])
32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CJZLnf026911
32, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:35:22 -0600
32, 22 -- Content-class: urn:content-classes:message
32, 22 -- MIME-Version: 1.0
32, 22 -- Content-Type: text/plain;
32, 22 -- charset="us-ascii"
32, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
32, 22 -- Subject: RE: [Microscopy] RE: TEM:cell culture with extracellular calcium - non-aqueous fixation
32, 22 -- Date: Mon, 12 Jan 2009 11:35:19 -0800
32, 22 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A040C29-at-dixie.bri.local}
32, 22 -- In-Reply-To: {200901121856.n0CIuwoH006559-at-ns.microscopy.com}
32, 22 -- X-MS-Has-Attach:
32, 22 -- X-MS-TNEF-Correlator:
32, 22 -- Thread-Topic: [Microscopy] RE: TEM:cell culture with extracellular calcium - non-aqueous fixation
32, 22 -- Thread-Index: Acl054ygcHZ11egoRkyDVWjg+NhE+wAA3RtA
32, 22 -- References: {200901121856.n0CIuwoH006559-at-ns.microscopy.com}
32, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com}
32, 22 -- To: {Microscopy-at-microscopy.com}
32, 22 -- Content-Transfer-Encoding: 8bit
32, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CJZLnf026911
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Mon, 12 Jan 2009 14:15:44 -0600
Subject: [Microscopy] RE: TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There was an old paper about anhydrous specimen preparation for TEM:
http://www.ncbi.nlm.nih.gov/pubmed/66323

Method used ethylene glycol, cellosolve and propylene oxide. Embedded in
Epon specimens were cut with knife filled with ethylene glycol. A while
ago I used this method and got some results.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
} Sent: Monday, January 12, 2009 10:59 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] TEM:cell culture with extracellular calcium
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
}
} Listers,
}
} I have a TEM project, where the researcher wants to observe
} their monolayer cell culture and the calcium crystals
} produced by those cells. The cells produce extracellular
} calcium crystals which dissolve readily in water.
} The usual fixation, post-fix, dehydration steps won't work .
} This situation is unlike anything I have dealt with before,
} including all my years of working with marine invertebrates.
} I am speculating that cryo methods may be the only answer.
} As always thanks in advance for any help possible.
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}
} ==============================Original
} Headers==============================
} 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20
} -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu
} [192.198.54.127])
} 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0CGw3WY025453
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:03 -0600
} 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
} 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP
} id C8AFDA0076
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:02 -0600 (CST)
} 5, 20 -- Received: from unmcnotes01.unmc.edu
} (host-137-197-103-35.unmc.edu [137.197.103.35])
} 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP
} id A012539804F
} 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:00 -0600 (CST)
} 5, 20 -- Subject: TEM:cell culture with extracellular calcium
} 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer:
} Lotus Notes Release 7.0.1 January 17, 2006 5, 20 --
} Message-ID:
} {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date:
} Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack:
} Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at
} 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0
} 5, 20 -- Content-type: text/plain; charset=US-ASCII
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 26 -- From DusevichV-at-umkc.edu Mon Jan 12 14:15:43 2009
8, 26 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CKFhDZ009316
8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 14:15:43 -0600
8, 26 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 26 -- Mon, 12 Jan 2009 14:15:42 -0600
8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 26 -- Content-class: urn:content-classes:message
8, 26 -- MIME-Version: 1.0
8, 26 -- Content-Type: text/plain;
8, 26 -- charset="us-ascii"
8, 26 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium
8, 26 -- Date: Mon, 12 Jan 2009 14:15:42 -0600
8, 26 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AC-at-KC-MSX1.kc.umkc.edu}
8, 26 -- In-Reply-To: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
8, 26 -- X-MS-Has-Attach:
8, 26 -- X-MS-TNEF-Correlator:
8, 26 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium
8, 26 -- thread-index: Acl01wj0f9lD3FrtTi+bEOThxFeWogAGlcKA
8, 26 -- References: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
8, 26 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 26 -- To: {tbargar-at-unmc.edu} , {microscopy-at-msa.microscopy.com} ,
8, 26 -- {Microscopy-at-microscopy.com}
8, 26 -- X-OriginalArrivalTime: 12 Jan 2009 20:15:42.0772 (UTC) FILETIME=[8B7A3740:01C974F2]
8, 26 -- Content-Transfer-Encoding: 8bit
8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CKFhDZ009316
==============================End of - Headers==============================




From: gnadamson-at-ucdavis.edu
Date: Mon, 12 Jan 2009 15:26:04 -0600
Subject: [Microscopy] Nanoplast resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

My ancient files have yielded a manufacturer for NANOPLAST FB 101. Google Rolf Bachhuber and a 2002 paper comes up with manufacturer in Germany. I'd type it out but my typing isn't to be trusted.

A publication from 'Agar Scientific' in May 1990 has references about melamine resins. That company was in the U.K. at the time.

I have an old Nanoplast kit from Polyscience in the U.S. I know they don't do any manufacturing and I have no idea if they still carry it.

Good luck,

Grete Adamson
EM Med Path
U.C. Davis

-----Original Message-----
X-from: k.sader-at-leeds.ac.uk [mailto:k.sader-at-leeds.ac.uk]
Sent: Monday, January 12, 2009 10:05 AM
To: Adamson, Grete

Hi,

I have been searching around for some time and I have not been able to find
a supplier with any Nanoplast (melamine) resin. Does anyone know what
company actually produced Nanoplast, if they are still operating, or if
there is a supplier who still has some?

Thanks,

Kasim Sader



----------------------
Dr Kasim Sader
SuperSTEM
Daresbury Laboratory
Warrington
WA4 4AD




==============================Original Headers==============================
10, 30 -- From K.Sader-at-leeds.ac.uk Mon Jan 12 11:56:48 2009
10, 30 -- Received: from mhost03c.leeds.ac.uk (mhost03c.leeds.ac.uk [129.11.76.167])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHulM7002354
10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 11:56:48 -0600
10, 30 -- Received: from APOLLO1.ds.leeds.ac.uk (apollo1.leeds.ac.uk [129.11.5.4])
10, 30 -- by mhost03c.leeds.ac.uk (8.14.3/8.14.3) with ESMTP id n0CHukS2019389
10, 30 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT)
10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 17:56:46 GMT
10, 30 -- Received: from dexbr1.ds.leeds.ac.uk (129.11.76.19) by APOLLO1.ds.leeds.ac.uk
10, 30 -- (129.11.5.4) with Microsoft SMTP Server id 8.1.311.2; Mon, 12 Jan 2009
10, 30 -- 17:56:45 +0000
10, 30 -- Received: from HERMES4.ds.leeds.ac.uk ([129.11.5.130]) by
10, 30 -- dexbr1.ds.leeds.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 Jan
10, 30 -- 2009 17:56:45 +0000
10, 30 -- Received: from 129.11.40.48 ([129.11.40.48]) by HERMES4.ds.leeds.ac.uk
10, 30 -- ([129.11.5.131]) via Exchange Front-End Server outlook.leeds.ac.uk
10, 30 -- ([129.11.5.3]) with Microsoft Exchange Server HTTP-DAV ; Mon, 12 Jan 2009
10, 30 -- 17:56:45 +0000
10, 30 -- User-Agent: Microsoft-Entourage/11.4.0.080122
10, 30 -- Date: Mon, 12 Jan 2009 17:56:44 +0000
10, 30 -- Subject: Nanoplast resin
10, 30 -- From: Kasim Sader {k.sader-at-leeds.ac.uk}
10, 30 -- To: {Microscopy-at-Microscopy.Com}
10, 30 -- Message-ID: {C59134DC.619C%k.sader-at-leeds.ac.uk}
10, 30 -- Thread-Topic: Nanoplast resin
10, 30 -- Thread-Index: Acl03yEuX9NSRODSEd218gAZ4zduOA==
10, 30 -- MIME-Version: 1.0
10, 30 -- Content-Type: text/plain; charset="US-ASCII"
10, 30 -- Content-Transfer-Encoding: 7bit
10, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:56:45.0245 (UTC) FILETIME=[21EC8ED0:01C974DF]
==============================End of - Headers==============================


==============================Original Headers==============================
23, 25 -- From gnadamson-at-ucdavis.edu Mon Jan 12 15:26:03 2009
23, 25 -- Received: from Mail.ucdsom.ucdavis.edu (mail.ucdsom.ucdavis.edu [169.237.87.32])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CLQ3pf024857
23, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 15:26:03 -0600
23, 25 -- Received: from Mail.ucdsom.ucdavis.edu ([169.237.87.32]) by
23, 25 -- Mail.ucdsom.ucdavis.edu ([169.237.87.32]) with mapi; Mon, 12 Jan 2009
23, 25 -- 13:26:29 -0800
23, 25 -- From: "Adamson, Grete" {gnadamson-at-ucdavis.edu}
23, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
23, 25 -- CC: "'k.sader-at-leeds.ac.uk'" {k.sader-at-leeds.ac.uk}
23, 25 -- Date: Mon, 12 Jan 2009 13:26:29 -0800
23, 25 -- Subject: RE: [Microscopy] Nanoplast resin
23, 25 -- Thread-Topic: [Microscopy] Nanoplast resin
23, 25 -- Thread-Index: Acl04FtAL+NoPsEYRYC3hsNvOUTuBQAGnZoA
23, 25 -- Message-ID: {FAB730F779268A468497EFF961DBBE11BA914134AD-at-Mail.ucdsom.ucdavis.edu}
23, 25 -- In-Reply-To: {200901121804.n0CI4qU1028997-at-ns.microscopy.com}
23, 25 -- Accept-Language: en-US
23, 25 -- Content-Language: en-US
23, 25 -- X-MS-Has-Attach:
23, 25 -- X-MS-TNEF-Correlator:
23, 25 -- acceptlanguage: en-US
23, 25 -- Content-Type: text/plain; charset="us-ascii"
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CLQ3pf024857
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Mon, 12 Jan 2009 15:59:23 -0600
Subject: [Microscopy] Old film etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings

This is a message I never thought I would be sending.

We are getting out of the film and paper photo business, and have a
lot of 'stuff' to give away or send to the landfill. Anyone with
questions about whether digital imaging for EM is for real, let this
be your wake-up call.

Most of what we have is old, probably not worth the cost to ship,
unless you are desperate, or curious.

We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572.
I don't even know what some of this was used for, if it rings your
bell, let me know and I can tell you more.

We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4
x5 sizes.

A little 4463 and a ton of SO-163. The SO-163 is old.

Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.

Most of this junk has been in a freezer. Some is pretty old and past
its expiration date, but if you are interested, let me know and we can
try to work something out. If I don't hear anything in the next week
or so, its outa here.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
14, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 12 15:59:23 2009
14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0CLxMKe006917
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 15:59:23 -0600
14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
14, 42 -- by localhost (Email Security Appliance) with SMTP id 7D4C918E05E
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT)
14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
14, 42 -- by mailin.deltacollege.edu (Email Security Appliance) with ESMTP id 726D520CAFD
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT)
14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
14, 42 -- with ESMTP id 44930245 for Microscopy-at-microscopy.com; Mon, 12 Jan 2009 13:59:19 -0800
14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
14, 42 -- ESMTP id KDDOD300.CM0 for {Microscopy-at-microscopy.com} ; Mon, 12
14, 42 -- Jan 2009 13:43:51 -0800
14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 13E398F74D38
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:19 -0800 (PST)
14, 42 -- X-Virus-Scanned: amavisd-new at
14, 42 -- X-Spam-Flag: NO
14, 42 -- X-Spam-Score: -2.324
14, 42 -- X-Spam-Level:
14, 42 -- X-Spam-Status: No, score=-2.324 tagged_above=-10 required=6 tests=[AWL=0.175,
14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
14, 42 -- with ESMTP id sAJT+MZ3mFOx for {Microscopy-at-microscopy.com} ;
14, 42 -- Mon, 12 Jan 2009 13:59:18 -0800 (PST)
14, 42 -- Received: from [172.20.4.106] (unknown [172.20.4.106])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id AEBE88F74D32
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:18 -0800 (PST)
14, 42 -- Message-Id: {7C9F6B4C-B8AF-43D7-86A6-495112B71078-at-deltacollege.edu}
14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
14, 42 -- To: Microscopy-at-microscopy.com
14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
14, 42 -- Content-Transfer-Encoding: 7bit
14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
14, 42 -- Subject: Old film etc
14, 42 -- Date: Mon, 12 Jan 2009 13:59:18 -0800
14, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Tue, 13 Jan 2009 01:21:13 -0600
Subject: [Microscopy] Re: Updating Label?... viaWWW: uranyl compounds are

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

It turned out that we have a material which emits alpha, beta and
gamma rays. I think the original labeling for uranyl compounds which
said "alpha emitter" should be changed. Especially, for the reason
that there might be microscopists who are using uranyl compounds in
their labs but, do not follow the Microscopy List.

Is there anybody in this group who is in a position and willing to
contact Nuclear Regulatory Commission on this subject?

Regards,
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Mon, Jan 12, 2009 at 8:26 PM, {tivol-at-caltech.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote:
}
} } Someone strongly pointed out that U is an alpha emitter, not a gamma
} } emitter, and I was not reading gamma radiation (but I was). You
} } pointed
} } out that it was the decay impurities that were the gamma emitters
} } and that
} } was what I was reading on my counter. I agree that U-238 and DU are
} } still
} } radioactive.
}
} } Here's my favorite. "depleted uranium is 40% less radioactive than
} } natural
} } uranium." That means 60% of the radioactivity is still there. I am
} } not
} } sure that includes gamma but probably.
}
}
} Dear Paul,
} Since the activities of the U isotopes are inversely proportional to
} their half-lives, and since the half lives of U235 and U234 are about
} 7 and 20,000 times shorter respectively than U238's, the amount of
} radiation from U234 is about equal to that from U238, and that from
} U235 is about 5% of that from the others, so your quote that DU has
} only 60% the activity of natural U checks out. I pointed out that
} ~1/4 of the U238 decays are to an excited state of Th234, which is ~50
} keV above the ground state, so pure U238 will produce some low-energy
} gammas, as will many of the daughters. The bottom line is that direct
} measurements performed correctly don't lie, so they are the best way
} to settle this issue once and for all.
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}

==============================Original Headers==============================
8, 32 -- From celikaktas-at-gmail.com Tue Jan 13 01:21:11 2009
8, 32 -- Received: from mu-out-0910.google.com (mu-out-0910.google.com [209.85.134.189])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0D7LAJI000663
8, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 01:21:10 -0600
8, 32 -- Received: by mu-out-0910.google.com with SMTP id w9so6630873mue.0
8, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 23:21:09 -0800 (PST)
8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 32 -- d=gmail.com; s=gamma;
8, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
8, 32 -- :from:to:content-type:content-transfer-encoding;
8, 32 -- bh=wjfEMkzAxlZHP279oRoyEi2BHmNZ5qmvW9E+/LODEuc=;
8, 32 -- b=E1VS580eiZKvpEWh5Swi392D4XXRNLvDCVuFEuSp9UqhBV1JvfuOvMIDnVoR1vNznc
8, 32 -- r5L1Z0TtXqt7mJXYCYcuaRGapCY8uHGnXv6W+VlNuOkBmNIfp97CLppTrQ9q9ppekELu
8, 32 -- kwwJ7gZg97W5DDFnjeF/OxGaHUrQ6UD82ohLY=
8, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 32 -- d=gmail.com; s=gamma;
8, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
8, 32 -- :content-transfer-encoding;
8, 32 -- b=ntMPorKNV/TSgRwuNzbJnEnwEgRXpiOCZr8l7jnSDPeacm7aCKVfux+bNhJKw49bri
8, 32 -- JcAVl8XZn7UmecRQPwL7o5bltCdYS+8v59joM0sIvDoKUmY8PGV2YorAmmiQ6/M6j/ll
8, 32 -- JGVoOwQH4/WN2HEF0ny5gehH0tcdQgk08a7+Q=
8, 32 -- MIME-Version: 1.0
8, 32 -- Received: by 10.103.217.7 with SMTP id u7mr3452314muq.125.1231831269725; Mon,
8, 32 -- 12 Jan 2009 23:21:09 -0800 (PST)
8, 32 -- Date: Tue, 13 Jan 2009 10:21:09 +0300
8, 32 -- Message-ID: {1075c5c10901122321l5c772174r4fedda4b0656a5-at-mail.gmail.com}
8, 32 -- Subject: Re: [Microscopy] Updating Label?... viaWWW: uranyl compounds are
8, 32 -- alpha emitters
8, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
8, 32 -- To: microscopy {Microscopy-at-microscopy.com}
8, 32 -- Content-Type: text/plain; charset=UTF-8
8, 32 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 13 Jan 2009 03:38:41 -0600
Subject: [Microscopy] RE: TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

How did you then dry your grids without leaving crystals?

Stéphane



----- Original Message ----
X-from: "DusevichV-at-umkc.edu" {DusevichV-at-umkc.edu}
To: nizets2-at-yahoo.com
Sent: Monday, January 12, 2009 9:19:38 PM

There was an old paper about anhydrous specimen preparation for TEM:
http://www.ncbi.nlm.nih.gov/pubmed/66323

Method used ethylene glycol, cellosolve and propylene oxide. Embedded in
Epon specimens were cut with knife filled with ethylene glycol. A while
ago I used this method and got some results.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax:  (816) 235-5524
Web:    http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
} Sent: Monday, January 12, 2009 10:59 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] TEM:cell culture with extracellular calcium
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy
} Society of America To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
}
} Listers,
}
} I have a TEM project, where the researcher wants to observe
} their monolayer cell culture and the calcium crystals
} produced by those cells. The cells produce extracellular
} calcium crystals which dissolve readily in water.
} The usual fixation, post-fix, dehydration steps won't work .
} This situation is unlike anything I have dealt with before,
} including all my years of working with marine invertebrates.
} I am speculating that cryo methods may be the only answer. 
} As always thanks in advance for any help possible.
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}  
} ==============================Original
} Headers==============================
} 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20
} -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu
} [192.198.54.127])
} 5, 20 --     by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0CGw3WY025453
} 5, 20 --     for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:03 -0600
} 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1])
} 5, 20 --     by Outbound.unmc.edu (Proprietary) with ESMTP
} id C8AFDA0076
} 5, 20 --     for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:02 -0600 (CST)
} 5, 20 -- Received: from unmcnotes01.unmc.edu
} (host-137-197-103-35.unmc.edu [137.197.103.35])
} 5, 20 --     by zixvpm02.unmc.edu (Proprietary) with ESMTP
} id A012539804F
} 5, 20 --     for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 10:58:00 -0600 (CST)
} 5, 20 -- Subject: TEM:cell culture with extracellular calcium
} 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer:
} Lotus Notes Release 7.0.1 January 17, 2006 5, 20 --
} Message-ID:
} {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date:
} Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack:
} Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at
} 01/12/2009 10:59:09 5, 20 --  AM 5, 20 -- MIME-Version: 1.0
} 5, 20 -- Content-type: text/plain; charset=US-ASCII
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 26 -- From DusevichV-at-umkc.edu Mon Jan 12 14:15:43 2009
8, 26 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
8, 26 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CKFhDZ009316
8, 26 --     for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 14:15:43 -0600
8, 26 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 26 --     Mon, 12 Jan 2009 14:15:42 -0600
8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 26 -- Content-class: urn:content-classes:message
8, 26 -- MIME-Version: 1.0
8, 26 -- Content-Type: text/plain;
8, 26 --     charset="us-ascii"
8, 26 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium
8, 26 -- Date: Mon, 12 Jan 2009 14:15:42 -0600
8, 26 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AC-at-KC-MSX1.kc.umkc.edu}
8, 26 -- In-Reply-To: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
8, 26 -- X-MS-Has-Attach:
8, 26 -- X-MS-TNEF-Correlator:
8, 26 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium
8, 26 -- thread-index: Acl01wj0f9lD3FrtTi+bEOThxFeWogAGlcKA
8, 26 -- References: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
8, 26 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 26 -- To: {tbargar-at-unmc.edu} , {microscopy-at-msa.microscopy.com} ,
8, 26 --        {Microscopy-at-microscopy.com}
8, 26 -- X-OriginalArrivalTime: 12 Jan 2009 20:15:42.0772 (UTC) FILETIME=[8B7A3740:01C974F2]
8, 26 -- Content-Transfer-Encoding: 8bit
8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CKFhDZ009316
==============================End of - Headers==============================






==============================Original Headers==============================
24, 23 -- From nizets2-at-yahoo.com Tue Jan 13 03:38:41 2009
24, 23 -- Received: from web110811.mail.gq1.yahoo.com (web110811.mail.gq1.yahoo.com [67.195.13.234])
24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0D9ceIv028716
24, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 03:38:40 -0600
24, 23 -- Received: (qmail 51735 invoked by uid 60001); 13 Jan 2009 09:38:39 -0000
24, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
24, 23 -- s=s1024; d=yahoo.com;
24, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
24, 23 -- b=WZ/fstX2SL7fNwcND9HJOkb+HHnMQWPrIRGFtWIxC3ramQWnjPyaWoF1w0ycmEk/3l6dZqh3ozcBl9MFqDznUQbSVUIamugOS36Tkka07luWlPVRYShYPngi6gAwpvtnsXzzzgSWE4HeT2tmRL1v86xp3sC6nfcF17Pv7Fo8tgI=;
24, 23 -- X-YMail-OSG: o9XbG70VM1mt1OYnAXQRD6U2ubiXED.mno5vQWXKjf16cV.8ucVWjlSr4Z5_DRY7yb6F.tDBPyUc56zh0k9JddAjj8TS9eS2NeEaf9HOCcnSYA2B.HMxdx2tbQKgIdTdKXJdSHsYgz3duwt_WPOiOKuuU.69n1eUA6drW.RSeiccF2lXK_qbPcFGhOUXjVxbMk8ZS4BcF8P2eELKCdY58ID7WbgOGHKK
24, 23 -- Received: from [80.122.101.100] by web110811.mail.gq1.yahoo.com via HTTP; Tue, 13 Jan 2009 01:38:39 PST
24, 23 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1
24, 23 -- References: {200901122019.n0CKJcVt015884-at-ns.microscopy.com}
24, 23 -- Date: Tue, 13 Jan 2009 01:38:39 -0800 (PST)
24, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com}
24, 23 -- Subject: Re: [Microscopy] RE: TEM:cell culture with extracellular calcium
24, 23 -- To: DusevichV-at-umkc.edu
24, 23 -- Cc: microscopy-at-microscopy.com
24, 23 -- MIME-Version: 1.0
24, 23 -- Content-Type: text/plain; charset=iso-8859-1
24, 23 -- Message-ID: {849685.51720.qm-at-web110811.mail.gq1.yahoo.com}
24, 23 -- Content-Transfer-Encoding: 8bit
24, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0D9ceIv028716
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 13 Jan 2009 04:05:07 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the
former being the most significant in terms of biological hazard [assuming
it's internalised]. However even natural uranium is weakly radioactive:
fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion
years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is
enriched for bomb production or for use as reactor fuel, it's the fissile
U-235* that’s wanted. However during enrichment U-234, being somewhat
similar in atomic mass, gets in with the U-235 fraction, so the left over
depleted uranium [DU, used in munitions casing and EM stains] is less
radioactive than even natural uranium. Uranyl salts used for EM staining are
now made from depleted uranium [which offers the lowest radioactivity], but
compared to enriched uranium both are relatively low in radioactivity in any
case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.

Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium
as it has even more U-238 and even less of the more radioactive isotopes
[i.e. the small U-234 fraction accounts for about half the radioactivity of
natural uranium]. U decay emits both alpha particles and beta+gamma rays.
The gamma dose will be quite small [depending on the mass of the uranyl
salts in your keep], but you should be able to detect it above background
with a crackle-crackle type Geiger counter. As gamma dose is a function of
mass & distance, keeping the small uranyl stock bottle well away from where
you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label,
as internal exposure after ingestion/inhalation is it's main toxicological
hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission
reaction occurs and things get scary] is apparently around 15 to 52kg [10 to
17cm diameter volume] - never tried it personally, but that’s a lot of U and
not something a microscopist need be concerned with.

In comparison plutonium-239 has a half-life of 24,000 years [ten times
faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is
187,500 more radioactive than 1g of U-238. There are long range gamma-rays
emitted from uranium as well as alpha-particles [the latter are only of
biological concern if the U is ingested]. Weapons grade enriched uranium has
an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100
times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this].
The gamma-ray activity will be about 40% of this [for enriched uranium]. Out
of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it
was in the stuff I used], thus it is significantly more radioactive compared
to natural [and depleted] uranium.

However for all practical purposes the radiation effects of depleted [and
even natural] uranium can generally be ignored when compared to background
radiation. This is largely the case for uranium, as being a heavy metal
analogue of lead, it is very toxic to life simply as a chemical. Like lead,
mercury and arsenic, uranium serves 'no useful biological function' and all
life-time studies find that kidney damage from uranium ingestion probably
occurs long before any radiation mutagenic effects would be seen [you only
die once, and as the natural U body burden increases the heavy metal
toxicity is likely to get you first].

It is generally considered that lead is far more chemically cyto-toxic than
uranium [largely due to U's far lower solubility and it's quick excretion
rate - although this leads to deposition in the kidney tubules that can
cause kidney damage]. Like lead, it is also excreted via the hair. Lead can
severely affect the nervous system and other biological pathways, but this
isn't seen with uranium [it's damage to kidneys being it's main toxic
effect, although authors of recent DU studies have suggested links to birth
defects and there's the long running controversy over gulf war syndrome]. It
seems that humans have to ingest 10s of grams of natural uranium before
adverse effects are seen in the short term. Animal studies suggest far lower
limits are a wise precaution though with this toxic heavy metal, as damage
has been seen in other organs [e.g. in the lung after inhalation of
'insoluble' enriched UO2 particles]. Natural uranium can deposit on the
bone, but the weak alpha-radiation dose is far too low to induce things like
leukaemia [in all probability]. So it is probably safer to be shot with a
depleted uranium bullet than a lead bullet [but both are best avoided].
Likewise lead shielding is probably potentially more toxic than the uranyl
salts it would be shielding. That's not to say uranium isn't very toxic,
just that soluble lead is even more toxic [hence it's ability to destroy the
Roman Empire from within].

Pelco [who supply the uranyl EM stain] state in there safety sheet that a
material must have a specific activity greater than 74 Becquerel per gram
(Bq/g) in order to be regarded as a radioactive material. One bequeral is
one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted
Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl
acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g],
although the biological hazard from far lower gamma/beta emission rate can
often be considered negligible compared to background or the alpha particle
emission should it become internalised in someone.

So I'd treat uranium with caution as a toxic heavy metal chemical rather
than a radioactive one [as that’s probably its greatest hazard]. Either way
it's hazard is by ingestion or inhalation, so handle with care as outlined
on the supplied safety sheet. External irradiation by the gamma & beta rays
if fairly insignificant from depleted uranium EM stains, and generally most
recommend simply storing in a metal can. It's daughter decay product: Radon
gas, can cause problems in enclosed areas where uranium is abundant in the
soil though.

Regards

Keith J Morris

*U-235 is an interesting isotope and as well as being fissile it can be
measured in extremely minute [trace] quantities using a technique known as
delayed neutron analysis.

http://www.cstl.nist.gov/projects/fy06/fhls0683903.pdf


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com]
Sent: 09 January 2009 00:27
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: abesenyo-at-ibilabs.com
Name: Alex Besenyo PhD

Organization: ibilabs

Title-Subject: [Filtered] uranyl compounds are alpha emitters only

Question: Question:

Is it true that the stuff we use has been somehow
depleted, so that it isn't as radioactive as "real" uranyl
salts? Or is this yet another old wive's tale of EM?!

Reply:

When we manufacture these compounds we purchase the raw uranium in a
depleted state from the government. There is no chance for error
here. We do not use natural uranium.

This means that the enrichable uranium U-235 has been removed.
The then U-238 which only emitts alpha radiation is procesed.

The term "depleted" means that U-235 has been removed.

If even by the slightest chance that U235 were present then every
alarm would go off in our facility because Beta and Gamma radiation
is detected.

I hope this answers everybodies concerns.

Our products are sold exclusively through a distributor network and
all of them have been instructed on this information.

I only responded when I saw the original post and I had to respond
before it got out of control.

Sincerely
Alex Besenyo PhD


Login Host: 74.173.69.139
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009
17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov
[130.202.238.72])
17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n090Jp6S032218
17, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52
-0600
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]}
17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
39, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jan 13 04:05:07 2009
39, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
39, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DA56tP012959
39, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 Jan 2009 04:05:06 -0600
39, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
39, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
39, 23 -- id 1LMg8v-0006zz-ST
39, 23 -- for Microscopy-at-Microscopy.Com; Tue, 13 Jan 2009 10:05:06 +0000
39, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
39, 23 -- To: {Microscopy-at-Microscopy.Com}
39, 23 -- References: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- Subject: RE: [Microscopy] viaWWW:Yes uranyl compounds do emit few gammas, betas as well
39, 23 -- Date: Tue, 13 Jan 2009 10:05:06 -0000
39, 23 -- Message-ID: {08E534FB0C6245979400D75365C7BAAF-at-CytoWhizz}
39, 23 -- MIME-Version: 1.0
39, 23 -- Content-Type: text/plain;
39, 23 -- charset="iso-8859-1"
39, 23 -- X-Mailer: Microsoft Office Outlook 11
39, 23 -- Thread-Index: Aclx8QYXX4MpJfSGTamN32m5ifY3bwAVUa0g
39, 23 -- In-Reply-To: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
39, 23 -- Content-Transfer-Encoding: 8bit
39, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DA56tP012959
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 13 Jan 2009 07:11:23 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all!

Really, I have the feeling that we are making an elephant out of a mouse.
How many times do you have to handle how much of depleted U?
2 times a year? 10 milligrams?
How do you weight your uranium salt? Do you pour all the content of the box on the bench, then take what you need? :-)
As someone said, I am more concerned about the chemical toxicity following ingestion (although even in this case it is probably fewer than few) than the radiations, except if you leave the box of U salt in one pocket of your blue jeans, which I wouldn't recommend (especially if you want children later).

Alternatively, I was infinitely more concerned about the election of Bush as a president than about the radiation of depleted U. Obviously I was right :-D
And there is no Bush-Hazard-Security-Agency, no rules to dispose of Bush in an environment-friendly way and no ALARE rules (as long as reasonably eligible).
Well it is never too late to start.
Oh, is it? :-D

Stéphane



----- Original Message ----
X-from: "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk}
To: nizets2-at-yahoo.com
Sent: Tuesday, January 13, 2009 11:12:29 AM

Hi all,

Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the
former being the most significant in terms of biological hazard [assuming
it's internalised]. However even natural uranium is weakly radioactive:
fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion
years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is
enriched for bomb production or for use as reactor fuel, it's the fissile
U-235* that’s wanted. However during enrichment U-234, being somewhat
similar in atomic mass, gets in with the U-235 fraction, so the left over
depleted uranium [DU, used in munitions casing and EM stains] is less
radioactive than even natural uranium. Uranyl salts used for EM staining are
now made from depleted uranium [which offers the lowest radioactivity], but
compared to enriched uranium both are relatively low in radioactivity in any
case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.

Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium
as it has even more U-238 and even less of the more radioactive isotopes
[i.e. the small U-234 fraction accounts for about half the radioactivity of
natural uranium]. U decay emits both alpha particles and beta+gamma rays.
The gamma dose will be quite small [depending on the mass of the uranyl
salts in your keep], but you should be able to detect it above background
with a crackle-crackle type Geiger counter. As gamma dose is a function of
mass & distance, keeping the small uranyl stock bottle well away from where
you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label,
as internal exposure after ingestion/inhalation is it's main toxicological
hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission
reaction occurs and things get scary] is apparently around 15 to 52kg [10 to
17cm diameter volume] - never tried it personally, but that’s a lot of U and
not something a microscopist need be concerned with. 

In comparison plutonium-239 has a half-life of 24,000 years [ten times
faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is
187,500 more radioactive than 1g of U-238. There are long range gamma-rays
emitted from uranium as well as alpha-particles [the latter are only of
biological concern if the U is ingested]. Weapons grade enriched uranium has
an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100
times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this].
The gamma-ray activity will be about 40% of this [for enriched uranium]. Out
of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it
was in the stuff I used], thus it is significantly more radioactive compared
to natural [and depleted] uranium.

However for all practical purposes the radiation effects of depleted [and
even natural] uranium can generally be ignored when compared to background
radiation. This is largely the case for uranium, as being a heavy metal
analogue of lead, it is very toxic to life simply as a chemical. Like lead,
mercury and arsenic, uranium serves 'no useful biological function' and all
life-time studies find that kidney damage from uranium ingestion probably
occurs long before any radiation mutagenic effects would be seen [you only
die once, and as the natural U body burden increases the heavy metal
toxicity is likely to get you first].

It is generally considered that lead is far more chemically cyto-toxic than
uranium [largely due to U's far lower solubility and it's quick excretion
rate - although this leads to deposition in the kidney tubules that can
cause kidney damage]. Like lead, it is also excreted via the hair. Lead can
severely affect the nervous system and other biological pathways, but this
isn't seen with uranium [it's damage to kidneys being it's main toxic
effect, although authors of recent DU studies have suggested links to birth
defects and there's the long running controversy over gulf war syndrome]. It
seems that humans have to ingest 10s of grams of natural uranium before
adverse effects are seen in the short term. Animal studies suggest far lower
limits are a wise precaution though with this toxic heavy metal, as damage
has been seen in other organs [e.g. in the lung after inhalation of
'insoluble' enriched UO2 particles]. Natural uranium can deposit on the
bone, but the weak alpha-radiation dose is far too low to induce things like
leukaemia [in all probability]. So it is probably safer to be shot with a
depleted uranium bullet than a lead bullet [but both are best avoided].
Likewise lead shielding is probably potentially more toxic than the uranyl
salts it would be shielding. That's not to say uranium isn't very toxic,
just that soluble lead is even more toxic [hence it's ability to destroy the
Roman Empire from within].

Pelco [who supply the uranyl EM stain] state in there safety sheet that a
material must have a specific activity greater than 74 Becquerel per gram
(Bq/g) in order to be regarded as a radioactive material. One bequeral is
one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted
Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl
acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g],
although the biological hazard from far lower gamma/beta emission rate can
often be considered negligible compared to background or the alpha particle
emission should it become internalised in someone.

So I'd treat uranium with caution as a toxic heavy metal chemical rather
than a radioactive one [as that’s probably its greatest hazard]. Either way
it's hazard is by ingestion or inhalation, so handle with care as outlined
on the supplied safety sheet. External irradiation by the gamma & beta rays
if fairly insignificant from depleted uranium EM stains, and generally most
recommend simply storing in a metal can. It's daughter decay product: Radon
gas, can cause problems in enclosed areas where uranium is abundant in the
soil though.

Regards

Keith J Morris

*U-235 is an interesting isotope and as well as being fissile it can be
measured in extremely minute [trace] quantities using a technique known as
delayed neutron analysis.

http://www.cstl.nist.gov/projects/fy06/fhls0683903.pdf


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com]
Sent: 09 January 2009 00:27
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both abesenyo-at-ibilabs.com as well as  the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: abesenyo-at-ibilabs.com
Name: Alex Besenyo PhD

Organization: ibilabs

Title-Subject: [Filtered] uranyl compounds are alpha emitters only

Question: Question:

Is it true that the stuff we use has been somehow
depleted, so that it isn't as radioactive as "real" uranyl
salts? Or is this yet another old wive's tale of EM?!

Reply:

When we manufacture these compounds we purchase the raw uranium in a
depleted state from the government. There is no chance for error
here. We do not use natural uranium.

This means that the enrichable uranium U-235 has been removed.
The then U-238 which only emitts alpha radiation is procesed.

The term "depleted" means that U-235 has been removed.

If even by the slightest chance that U235 were present then every
alarm would go off in our facility because Beta and Gamma radiation
is detected.

I hope this answers everybodies concerns.

Our products are sold exclusively through a distributor network and
all of them have been instructed on this information.

I only responded when I saw the original post and I had to respond
before it got out of control.

Sincerely
Alex Besenyo PhD


  Login Host: 74.173.69.139
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Thu Jan  8 18:19:52 2009
17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov
[130.202.238.72])
17, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n090Jp6S032218
17, 11 --     for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52
-0600
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]}
17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
39, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jan 13 04:05:07 2009
39, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
39, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DA56tP012959
39, 23 --     for {Microscopy-at-Microscopy.Com} ; Tue, 13 Jan 2009 04:05:06 -0600
39, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
39, 23 --     by morse.well.ox.ac.uk with esmtp (Exim 4.52)
39, 23 --     id 1LMg8v-0006zz-ST
39, 23 --     for Microscopy-at-Microscopy.Com; Tue, 13 Jan 2009 10:05:06 +0000
39, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
39, 23 -- To: {Microscopy-at-Microscopy.Com}
39, 23 -- References: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- Subject: RE: [Microscopy] viaWWW:Yes  uranyl compounds do emit few gammas, betas as well
39, 23 -- Date: Tue, 13 Jan 2009 10:05:06 -0000
39, 23 -- Message-ID: {08E534FB0C6245979400D75365C7BAAF-at-CytoWhizz}
39, 23 -- MIME-Version: 1.0
39, 23 -- Content-Type: text/plain;
39, 23 --     charset="iso-8859-1"
39, 23 -- X-Mailer: Microsoft Office Outlook 11
39, 23 -- Thread-Index: Aclx8QYXX4MpJfSGTamN32m5ifY3bwAVUa0g
39, 23 -- In-Reply-To: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
39, 23 -- Content-Transfer-Encoding: 8bit
39, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DA56tP012959
==============================End of - Headers==============================






==============================Original Headers==============================
57, 22 -- From nizets2-at-yahoo.com Tue Jan 13 07:11:23 2009
57, 22 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239])
57, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0DDBMqm009455
57, 22 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 07:11:22 -0600
57, 22 -- Received: (qmail 20923 invoked by uid 60001); 13 Jan 2009 13:11:22 -0000
57, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
57, 22 -- s=s1024; d=yahoo.com;
57, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
57, 22 -- b=AkrMz6GOq46CXKK5pOahDO6ZAfPPx48th9zUS4KULg0103NI1ifS/Zu4WQcIq9wOWLKYF3OO1YZ0dt85zbmE8FcB9bLkv6pP9Hlqm4LU3TxczJUrh6vJ831tTsMFJTsVcPu3d3VnEVudGcCV46iJvZ4S2VZXnhaGLhsxvsG0Tb0=;
57, 22 -- X-YMail-OSG: Zk.PogUVM1kVvQOYpqeXXnlFegUuBHQSs4XK0E87Nd8rz9zPBGBDGggYS18nRO3I5qe6z1y4aJX8L.3g7JMsH0hfefy8cMoX5gdFUcEdg_IpPdXzZl8e9M0hRcD65TsRPyAVZGRvqSOU_m7no6fnPXvwOLR1U7o578vTTjlpHMGglKoYzYIdJ7UAMwm7jQChIxQJCS8dDKLBbKv4ru.Vo4qr7pLEJ.jK
57, 22 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Tue, 13 Jan 2009 05:11:22 PST
57, 22 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1
57, 22 -- References: {200901131012.n0DACTSC020042-at-ns.microscopy.com}
57, 22 -- Date: Tue, 13 Jan 2009 05:11:22 -0800 (PST)
57, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
57, 22 -- Subject: uranyl compounds do emit few gammas, betas as well - Who cares?
57, 22 -- To: microscopy-at-microscopy.com
57, 22 -- MIME-Version: 1.0
57, 22 -- Content-Type: text/plain; charset=utf-8
57, 22 -- Message-ID: {380151.20813.qm-at-web110816.mail.gq1.yahoo.com}
57, 22 -- Content-Transfer-Encoding: 8bit
57, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DDBMqm009455
==============================End of - Headers==============================




From: WAHeeschen-at-dow.com
Date: Tue, 13 Jan 2009 08:36:47 -0600
Subject: [Microscopy] Old film etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks:
Old photo/darkroom supplies, etc., may find a happy home in art schools.
I have a young artist friend who is taking a photography class and the
instructor is insisting that she start with film in order to understand
the fundamentals of photography - particularly for B/W. Without getting
into arguments about log/linear behavior, benefits of one over the
other, etc., the point is that these folks may be able to utilize film
supplies and are likely to be very appreciative of a cheap/free source.
They may not have much use for the Type xxx film, but some of the other
supplies could be valuable.
Best Regards,
Bill
William A. Heeschen
Microscopy, Digital Imaging
1897 Bldg, E-84
Dow Chemical
Midland, MI 48667
mailto:waheeschen-at-dow.com

-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Monday, January 12, 2009 5:04 PM
To: Heeschen, Bill (WA)

Greetings

This is a message I never thought I would be sending.

We are getting out of the film and paper photo business, and have a
lot of 'stuff' to give away or send to the landfill. Anyone with
questions about whether digital imaging for EM is for real, let this
be your wake-up call.

Most of what we have is old, probably not worth the cost to ship,
unless you are desperate, or curious.

We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572.
I don't even know what some of this was used for, if it rings your
bell, let me know and I can tell you more.

We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4
x5 sizes.

A little 4463 and a ton of SO-163. The SO-163 is old.

Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.

Most of this junk has been in a freezer. Some is pretty old and past
its expiration date, but if you are interested, let me know and we can
try to work something out. If I don't hear anything in the next week
or so, its outa here.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original
Headers==============================
14, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 12 15:59:23 2009
14, 42 -- Received: from mailin.deltacollege.edu
(mailin.deltacollege.edu [207.62.178.150])
14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n0CLxMKe006917
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
15:59:23 -0600
14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain
[127.0.0.1])
14, 42 -- by localhost (Email Security Appliance) with SMTP id
7D4C918E05E
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
21:34:33 +0000 (GMT)
14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us
[207.62.178.236])
14, 42 -- by mailin.deltacollege.edu (Email Security Appliance)
with ESMTP id 726D520CAFD
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
21:34:33 +0000 (GMT)
14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
14, 42 -- with ESMTP id 44930245 for Microscopy-at-microscopy.com; Mon,
12 Jan 2009 13:59:19 -0800
14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server
4.15) with
14, 42 -- ESMTP id KDDOD300.CM0 for
{Microscopy-at-microscopy.com} ; Mon, 12
14, 42 -- Jan 2009 13:43:51 -0800
14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id
13E398F74D38
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
13:59:19 -0800 (PST)
14, 42 -- X-Virus-Scanned: amavisd-new at
14, 42 -- X-Spam-Flag: NO
14, 42 -- X-Spam-Score: -2.324
14, 42 -- X-Spam-Level:
14, 42 -- X-Spam-Status: No, score=-2.324 tagged_above=-10 required=6
tests=[AWL=0.175,
14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1])
(amavisd-new, port 10024)
14, 42 -- with ESMTP id sAJT+MZ3mFOx for
{Microscopy-at-microscopy.com} ;
14, 42 -- Mon, 12 Jan 2009 13:59:18 -0800 (PST)
14, 42 -- Received: from [172.20.4.106] (unknown [172.20.4.106])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id
AEBE88F74D32
14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009
13:59:18 -0800 (PST)
14, 42 -- Message-Id:
{7C9F6B4C-B8AF-43D7-86A6-495112B71078-at-deltacollege.edu}
14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
14, 42 -- To: Microscopy-at-microscopy.com
14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
14, 42 -- Content-Transfer-Encoding: 7bit
14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
14, 42 -- Subject: Old film etc
14, 42 -- Date: Mon, 12 Jan 2009 13:59:18 -0800
14, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of -
Headers==============================


==============================Original Headers==============================
21, 34 -- From WAHeeschen-at-dow.com Tue Jan 13 08:36:46 2009
21, 34 -- Received: from mail85.messagelabs.com (mail85.messagelabs.com [216.82.241.211])
21, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DEaks3030832
21, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 08:36:46 -0600
21, 34 -- X-VirusChecked: Checked
21, 34 -- X-Env-Sender: WAHeeschen-at-dow.com
21, 34 -- X-Msg-Ref: server-3.tower-85.messagelabs.com!1231857396!33256808!9
21, 34 -- X-StarScan-Version: 6.0.0; banners=-,-,-
21, 34 -- X-Originating-IP: [216.99.65.22]
21, 34 -- Received: (qmail 24830 invoked from network); 13 Jan 2009 14:36:45 -0000
21, 34 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22)
21, 34 -- by server-3.tower-85.messagelabs.com with RC4-SHA encrypted SMTP; 13 Jan 2009 14:36:45 -0000
21, 34 -- Received: from USMDLMDOWX026.dow.com ([163.198.215.57]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830);
21, 34 -- Tue, 13 Jan 2009 09:36:37 -0500
21, 34 -- Content-class: urn:content-classes:message
21, 34 -- MIME-Version: 1.0
21, 34 -- Content-Type: text/plain;
21, 34 -- charset="US-ASCII"
21, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 34 -- Subject: RE: [Microscopy] Old film etc
21, 34 -- Date: Tue, 13 Jan 2009 09:36:37 -0500
21, 34 -- Message-ID: {9FAC91DDE67EF3448238E5E33D3E5AEF01C6B129-at-USMDLMDOWX026.dow.com}
21, 34 -- In-Reply-To: {200901122204.n0CM47gR016422-at-ns.microscopy.com}
21, 34 -- X-MS-Has-Attach:
21, 34 -- X-MS-TNEF-Correlator:
21, 34 -- Thread-Topic: [Microscopy] Old film etc
21, 34 -- Thread-Index: Acl1AcbMkH3Log7TSe6nWCmmqyEhZgAiFHUg
21, 34 -- References: {200901122204.n0CM47gR016422-at-ns.microscopy.com}
21, 34 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com}
21, 34 -- To: {jkrupp-at-deltacollege.edu}
21, 34 -- Cc: {Microscopy-at-microscopy.com}
21, 34 -- X-OriginalArrivalTime: 13 Jan 2009 14:36:37.0775 (UTC) FILETIME=[5753C9F0:01C9758C]
21, 34 -- Content-Transfer-Encoding: 8bit
21, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DEaks3030832
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 13 Jan 2009 08:54:32 -0600
Subject: [Microscopy] TEM:cell culture with extracellular calcium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ethylene glycol evaporates without leaving crystals. Just like water, but slower.

Vladimir


} Hi!
}
} How did you then dry your grids without leaving crystals?
}
} Stéphane
}
}
}
} ----- Original Message ----
} From: "DusevichV-at-umkc.edu" {DusevichV-at-umkc.edu}
} To: nizets2-at-yahoo.com
} Sent: Monday, January 12, 2009 9:19:38 PM
} Subject: [Microscopy] RE: TEM:cell culture with extracellular calcium
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy
} Society of America To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} There was an old paper about anhydrous specimen preparation for TEM:
} http://www.ncbi.nlm.nih.gov/pubmed/66323
}
} Method used ethylene glycol, cellosolve and propylene oxide.
} Embedded in Epon specimens were cut with knife filled with
} ethylene glycol. A while ago I used this method and got some results.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax:  (816) 235-5524
} Web:    http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
} } Sent: Monday, January 12, 2009 10:59 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] TEM:cell culture with extracellular calcium
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of
} } America To  Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} }
} } Listers,
} }
} } I have a TEM project, where the researcher wants to observe their
} } monolayer cell culture and the calcium crystals produced by those
} } cells. The cells produce extracellular calcium crystals
} which dissolve
} } readily in water.
} } The usual fixation, post-fix, dehydration steps won't work .
} } This situation is unlike anything I have dealt with before,
} including
} } all my years of working with marine invertebrates.
} } I am speculating that cryo methods may be the only answer.
} As always
} } thanks in advance for any help possible.
} }
} } Tom Bargar
} } University of Nebraska Medical Center
} } Core Electron Microscopy Research Facility
} } 986395 Nebraska Medical Center
} } Omaha, NE 68198-6395
} } 402-559-7347
} } tbargar-at-unmc.edu
} }
} }  
} } ==============================Original
} } Headers==============================
} } 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20
} } -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu
} } [192.198.54.127])
} } 5, 20 --     by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP
} } id n0CGw3WY025453 5, 20 --     for
} {Microscopy-at-microscopy.com} ; Mon,
} } 12 Jan
} } 2009 10:58:03 -0600
} } 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM
} [127.0.0.1]) 5, 20
} } --     by Outbound.unmc.edu (Proprietary) with ESMTP id
} C8AFDA0076 5,
} } 20 --     for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} } 2009 10:58:02 -0600 (CST)
} } 5, 20 -- Received: from unmcnotes01.unmc.edu
} } (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 --     by
} } zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F
} 5, 20 --    
} } for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} } 2009 10:58:00 -0600 (CST)
} } 5, 20 -- Subject: TEM:cell culture with extracellular
} calcium 5, 20 --
} } To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer:
} } Lotus Notes Release 7.0.1 January 17, 2006 5, 20 --
} } Message-ID:
} } {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu}
} } 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date:
} } Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack:
} } Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at
} } 01/12/2009 10:59:09 5, 20 --  AM 5, 20 -- MIME-Version: 1.0
} 5, 20 --
} } Content-type: text/plain; charset=US-ASCII
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original
} Headers==============================
} 8, 26 -- From DusevichV-at-umkc.edu Mon Jan 12 14:15:43 2009 8,
} 26 -- Received: from kc-msxproto3.kc.umkc.edu
} (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 8, 26 --     by
} ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0CKFhDZ009316 8, 26 --     for {Microscopy-at-microscopy.com} ;
} Mon, 12 Jan 2009 14:15:43 -0600 8, 26 -- Received: from
} KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto3.kc.umkc.edu with Microsoft
} SMTPSVC(6.0.3790.3959); 8, 26 --     Mon, 12 Jan 2009
} 14:15:42 -0600 8, 26 -- X-MimeOLE: Produced By Microsoft
} Exchange V6.5 8, 26 -- Content-class:
} urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26
} -- Content-Type: text/plain; 8, 26 --     charset="us-ascii"
} 8, 26 -- Subject: RE: [Microscopy] TEM:cell culture with
} extracellular calcium 8, 26 -- Date: Mon, 12 Jan 2009
} 14:15:42 -0600 8, 26 -- Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AC-at-KC-MSX1.kc.umkc.edu}
} 8, 26 -- In-Reply-To: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
} 8, 26 -- X-MS-Has-Attach:
} 8, 26 -- X-MS-TNEF-Correlator:
} 8, 26 -- Thread-Topic: [Microscopy] TEM:cell culture with
} extracellular calcium 8, 26 -- thread-index:
} Acl01wj0f9lD3FrtTi+bEOThxFeWogAGlcKA
} 8, 26 -- References: {200901121658.n0CGwj2i026350-at-ns.microscopy.com}
} 8, 26 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8,
} 26 -- To: {tbargar-at-unmc.edu} ,
} {microscopy-at-msa.microscopy.com} , 8, 26 --       
} {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime:
} 12 Jan 2009 20:15:42.0772 (UTC) FILETIME=[8B7A3740:01C974F2]
} 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 --
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n0CKFhDZ009316
} ==============================End of -
} Headers==============================
}
}
}
}
}


==============================Original Headers==============================
5, 25 -- From DusevichV-at-umkc.edu Tue Jan 13 08:54:31 2009
5, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DEsVsX013464
5, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 08:54:31 -0600
5, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
5, 25 -- Tue, 13 Jan 2009 08:54:31 -0600
5, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 25 -- Content-class: urn:content-classes:message
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Type: text/plain;
5, 25 -- charset="iso-8859-1"
5, 25 -- Subject: RE: [Microscopy] RE: TEM:cell culture with extracellular calcium
5, 25 -- Date: Tue, 13 Jan 2009 08:54:30 -0600
5, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AE-at-KC-MSX1.kc.umkc.edu}
5, 25 -- In-Reply-To: {849685.51720.qm-at-web110811.mail.gq1.yahoo.com}
5, 25 -- X-MS-Has-Attach:
5, 25 -- X-MS-TNEF-Correlator:
5, 25 -- Thread-Topic: [Microscopy] RE: TEM:cell culture with extracellular calcium
5, 25 -- thread-index: Acl1Y6Yv1MtLcyy8RBSCZeh+XaJxNAAKwEYQ
5, 25 -- References: {200901122019.n0CKJcVt015884-at-ns.microscopy.com} {849685.51720.qm-at-web110811.mail.gq1.yahoo.com}
5, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
5, 25 -- To: {microscopy-at-microscopy.com}
5, 25 -- X-OriginalArrivalTime: 13 Jan 2009 14:54:31.0083 (UTC) FILETIME=[D71197B0:01C9758E]
5, 25 -- Content-Transfer-Encoding: 8bit
5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DEsVsX013464
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 13 Jan 2009 08:59:55 -0600
Subject: [Microscopy] RE: Old film etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good point, although not necessarily the case. The Art and journalism
deparments here have gone completely digital. Even strictly art
photography, the kind that uses high silver content paper and so
forth. They didn't even want a Durst enlarger.
Anybody on the list looking for a Durst Laborator enlarger in
excellent condition? With printing easel, extra lenses, etc.?

Phil

}
} Folks:
} Old photo/darkroom supplies, etc., may find a happy home in art schools.
} I have a young artist friend who is taking a photography class and the
} instructor is insisting that she start with film in order to understand
} the fundamentals of photography - particularly for B/W. Without getting
} into arguments about log/linear behavior, benefits of one over the
} other, etc., the point is that these folks may be able to utilize film
} supplies and are likely to be very appreciative of a cheap/free source.
} They may not have much use for the Type xxx film, but some of the other
} supplies could be valuable.
} Best Regards,
} Bill
} William A. Heeschen
} Microscopy, Digital Imaging
} 1897 Bldg, E-84
} Dow Chemical
} Midland, MI 48667
} mailto:waheeschen-at-dow.com
}
} -----Original Message-----
} X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
} Sent: Monday, January 12, 2009 5:04 PM
} To: Heeschen, Bill (WA)
} Subject: [Microscopy] Old film etc
}
---
}
} Greetings
}
} This is a message I never thought I would be sending.
}
} We are getting out of the film and paper photo business, and have a
} lot of 'stuff' to give away or send to the landfill. Anyone with
} questions about whether digital imaging for EM is for real, let this
} be your wake-up call.
}
} Most of what we have is old, probably not worth the cost to ship,
} unless you are desperate, or curious.
}
} We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572.
} I don't even know what some of this was used for, if it rings your
} bell, let me know and I can tell you more.
}
} We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4
} x5 sizes.
}
} A little 4463 and a ton of SO-163. The SO-163 is old.
}
} Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.
}
} Most of this junk has been in a freezer. Some is pretty old and past
} its expiration date, but if you are interested, let me know and we can
} try to work something out. If I don't hear anything in the next week
} or so, its outa here.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 25 -- From oshel1pe-at-cmich.edu Tue Jan 13 08:59:55 2009
4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DExs4e026270
4, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 08:59:55 -0600
4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0DExhQX000442
4, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 09:59:53 -0500
4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Tue, 13 Jan 2009 09:58:51 -0500
4, 25 -- Mime-Version: 1.0
4, 25 -- Message-Id: {f06240805c5925b610675-at-[141.209.160.249]}
4, 25 -- In-Reply-To: {200901131442.n0DEgQst004798-at-ns.microscopy.com}
4, 25 -- References: {200901131442.n0DEgQst004798-at-ns.microscopy.com}
4, 25 -- Date: Tue, 13 Jan 2009 09:58:49 -0500
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 25 -- Subject: [Microscopy] RE: Old film etc
4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 25 -- X-OriginalArrivalTime: 13 Jan 2009 14:58:51.0969 (UTC) FILETIME=[7291A310:01C9758F]
4, 25 -- X-Canit-CHI2: 0.00
4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 25 -- X-CanItPRO-Stream: default
4, 25 -- X-Canit-Stats-ID: 7380497 - 047461f10392
4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 13 Jan 2009 11:40:14 -0600
Subject: [Microscopy] viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hello,
}
} It turned out

No, it does not. Can you read other, more meaningful postings?

Vladimir


that we have a material which emits alpha, beta
} and gamma rays. I think the original labeling for uranyl
} compounds which said "alpha emitter" should be changed.
} Especially, for the reason that there might be microscopists
} who are using uranyl compounds in their labs but, do not
} follow the Microscopy List.
}
} Is there anybody in this group who is in a position and
} willing to contact Nuclear Regulatory Commission on this subject?
}
} Regards,
} Ayten.
}
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
}
} On Mon, Jan 12, 2009 at 8:26 PM, {tivol-at-caltech.edu} wrote:
} }
} }
} }
} }
} --------------------------------------------------------------
} --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} }
} } On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote:
} }
} } } Someone strongly pointed out that U is an alpha emitter,
} not a gamma
} } } emitter, and I was not reading gamma radiation (but I was). You
} } } pointed
} } } out that it was the decay impurities that were the gamma emitters
} } } and that
} } } was what I was reading on my counter. I agree that U-238
} and DU are
} } } still
} } } radioactive.
} }
} } } Here's my favorite. "depleted uranium is 40% less radioactive than
} } } natural
} } } uranium." That means 60% of the radioactivity is still
} there. I am
} } } not
} } } sure that includes gamma but probably.
} }
} }
} } Dear Paul,
} } Since the activities of the U isotopes are inversely
} proportional to
} } their half-lives, and since the half lives of U235 and U234
} are about
} } 7 and 20,000 times shorter respectively than U238's, the amount of
} } radiation from U234 is about equal to that from U238, and that from
} } U235 is about 5% of that from the others, so your quote that DU has
} } only 60% the activity of natural U checks out. I pointed out that
} } ~1/4 of the U238 decays are to an excited state of Th234,
} which is ~50
} } keV above the ground state, so pure U238 will produce some
} low-energy
} } gammas, as will many of the daughters. The bottom line is
} that direct
} } measurements performed correctly don't lie, so they are the best way
} } to settle this issue once and for all.
} } Yours,
} } Bill Tivol, PhD
} } EM Scientist
} } Ultrafast EM Facility
} } Noyes Laboratory, MC 127-72
} } California Institute of Technology
} } Pasadena CA 91125
} } (626) 395-8833
} } tivol-at-caltech.edu
} }
} }
}
} ==============================Original
} Headers==============================
} 8, 32 -- From celikaktas-at-gmail.com Tue Jan 13 01:21:11 2009
} 8, 32 -- Received: from mu-out-0910.google.com
} (mu-out-0910.google.com [209.85.134.189])
} 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0D7LAJI000663
} 8, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan
} 2009 01:21:10 -0600
} 8, 32 -- Received: by mu-out-0910.google.com with SMTP id
} w9so6630873mue.0
} 8, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan
} 2009 23:21:09 -0800 (PST)
} 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 8, 32 -- d=gmail.com; s=gamma;
} 8, 32 --
} h=domainkey-signature:mime-version:received:date:message-id:subject
} 8, 32 -- :from:to:content-type:content-transfer-encoding;
} 8, 32 -- bh=wjfEMkzAxlZHP279oRoyEi2BHmNZ5qmvW9E+/LODEuc=;
} 8, 32 --
} b=E1VS580eiZKvpEWh5Swi392D4XXRNLvDCVuFEuSp9UqhBV1JvfuOvMIDnVoR1vNznc
} 8, 32 --
} r5L1Z0TtXqt7mJXYCYcuaRGapCY8uHGnXv6W+VlNuOkBmNIfp97CLppTrQ9q9ppekELu
} 8, 32 -- kwwJ7gZg97W5DDFnjeF/OxGaHUrQ6UD82ohLY=
} 8, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 8, 32 -- d=gmail.com; s=gamma;
} 8, 32 --
} h=mime-version:date:message-id:subject:from:to:content-type
} 8, 32 -- :content-transfer-encoding;
} 8, 32 --
} b=ntMPorKNV/TSgRwuNzbJnEnwEgRXpiOCZr8l7jnSDPeacm7aCKVfux+bNhJKw49bri
} 8, 32 --
} JcAVl8XZn7UmecRQPwL7o5bltCdYS+8v59joM0sIvDoKUmY8PGV2YorAmmiQ6/M6j/ll
} 8, 32 -- JGVoOwQH4/WN2HEF0ny5gehH0tcdQgk08a7+Q=
} 8, 32 -- MIME-Version: 1.0
} 8, 32 -- Received: by 10.103.217.7 with SMTP id
} u7mr3452314muq.125.1231831269725; Mon,
} 8, 32 -- 12 Jan 2009 23:21:09 -0800 (PST)
} 8, 32 -- Date: Tue, 13 Jan 2009 10:21:09 +0300
} 8, 32 -- Message-ID:
} {1075c5c10901122321l5c772174r4fedda4b0656a5-at-mail.gmail.com}
} 8, 32 -- Subject: Re: [Microscopy] Updating Label?... viaWWW:
} uranyl compounds are
} 8, 32 -- alpha emitters
} 8, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
} 8, 32 -- To: microscopy {Microscopy-at-microscopy.com}
} 8, 32 -- Content-Type: text/plain; charset=UTF-8
} 8, 32 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Tue Jan 13 09:12:59 2009
7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DFCxeO009341
7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 09:12:59 -0600
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Tue, 13 Jan 2009 09:12:59 -0600
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] Re: Updating Label?... viaWWW: uranyl compounds are
7, 25 -- Date: Tue, 13 Jan 2009 09:12:59 -0600
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AF-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200901130722.n0D7M7G5001697-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] Re: Updating Label?... viaWWW: uranyl compounds are
7, 25 -- thread-index: Acl1T6VuVvBucRIWSfWbcChvYMJnGgAQY0fQ
7, 25 -- References: {200901130722.n0D7M7G5001697-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 13 Jan 2009 15:12:59.0426 (UTC) FILETIME=[6BB15020:01C97591]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DFCxeO009341
==============================End of - Headers==============================

From mathewokon-at-sify.com Tue Jan 13 10:44:28 2009
Return-Path: {mathewokon-at-sify.com}
Received: from vetbrands.com.br (smtp-gw.vetbrands.com.br [201.63.46.162])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DGiRNe028666;
Tue, 13 Jan 2009 10:44:28 -0600
BrmaOutput: [41.222.67.140]
Received: from User ([41.222.67.140])
(authenticated bits=0)
by vetbrands.com.br (8.12.11.20060308/8.12.11) with ESMTP id n0DC3hlv024740;
Tue, 13 Jan 2009 10:03:47 -0200
Message-Id: {200901131203.n0DC3hlv024740-at-vetbrands.com.br}
Reply-To: {mathew_okon1-at-yahoo.es}

Indeed, from Pelco's measurements of their solution I estimate that if you held a 25g bottle of their uranyl acetate against your skin for an entire year you would receive about 3.5 times the annual dose* you would get from background radiation sources [over the same year] - about 1,300 mRem. This would largely be from the gamma radiation [as you can assume the beta-rays and alpha particles are blocked by the glass jar, i.e. you kept the lid on, and your skin surface].

Keith

*Annual dose assumed to be 360 mRem [18% man made + 82% natural]. A radiation worker is allowed 5,000 mRem maximum occupational exposure.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 13 January 2009 13:19
To: kjmorris-at-well.ox.ac.uk

Dear all!

Really, I have the feeling that we are making an elephant out of a mouse.
How many times do you have to handle how much of depleted U?
2 times a year? 10 milligrams?
How do you weight your uranium salt? Do you pour all the content of the box on the bench, then take what you need? :-)
As someone said, I am more concerned about the chemical toxicity following ingestion (although even in this case it is probably fewer than few) than the radiations, except if you leave the box of U salt in one pocket of your blue jeans, which I wouldn't recommend (especially if you want children later).

Alternatively, I was infinitely more concerned about the election of Bush as a president than about the radiation of depleted U. Obviously I was right :-D
And there is no Bush-Hazard-Security-Agency, no rules to dispose of Bush in an environment-friendly way and no ALARE rules (as long as reasonably eligible).
Well it is never too late to start.
Oh, is it? :-D

Stéphane



----- Original Message ----
X-from: "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk}
To: nizets2-at-yahoo.com
Sent: Tuesday, January 13, 2009 11:12:29 AM

Hi all,

Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the
former being the most significant in terms of biological hazard [assuming
it's internalised]. However even natural uranium is weakly radioactive:
fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion
years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is
enriched for bomb production or for use as reactor fuel, it's the fissile
U-235* that’s wanted. However during enrichment U-234, being somewhat
similar in atomic mass, gets in with the U-235 fraction, so the left over
depleted uranium [DU, used in munitions casing and EM stains] is less
radioactive than even natural uranium. Uranyl salts used for EM staining are
now made from depleted uranium [which offers the lowest radioactivity], but
compared to enriched uranium both are relatively low in radioactivity in any
case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.

Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium
as it has even more U-238 and even less of the more radioactive isotopes
[i.e. the small U-234 fraction accounts for about half the radioactivity of
natural uranium]. U decay emits both alpha particles and beta+gamma rays.
The gamma dose will be quite small [depending on the mass of the uranyl
salts in your keep], but you should be able to detect it above background
with a crackle-crackle type Geiger counter. As gamma dose is a function of
mass & distance, keeping the small uranyl stock bottle well away from where
you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label,
as internal exposure after ingestion/inhalation is it's main toxicological
hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission
reaction occurs and things get scary] is apparently around 15 to 52kg [10 to
17cm diameter volume] - never tried it personally, but that’s a lot of U and
not something a microscopist need be concerned with.Â

In comparison plutonium-239 has a half-life of 24,000 years [ten times
faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is
187,500 more radioactive than 1g of U-238. There are long range gamma-rays
emitted from uranium as well as alpha-particles [the latter are only of
biological concern if the U is ingested]. Weapons grade enriched uranium has
an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100
times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this].
The gamma-ray activity will be about 40% of this [for enriched uranium]. Out
of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it
was in the stuff I used], thus it is significantly more radioactive compared
to natural [and depleted] uranium.

However for all practical purposes the radiation effects of depleted [and
even natural] uranium can generally be ignored when compared to background
radiation. This is largely the case for uranium, as being a heavy metal
analogue of lead, it is very toxic to life simply as a chemical. Like lead,
mercury and arsenic, uranium serves 'no useful biological function' and all
life-time studies find that kidney damage from uranium ingestion probably
occurs long before any radiation mutagenic effects would be seen [you only
die once, and as the natural U body burden increases the heavy metal
toxicity is likely to get you first].

It is generally considered that lead is far more chemically cyto-toxic than
uranium [largely due to U's far lower solubility and it's quick excretion
rate - although this leads to deposition in the kidney tubules that can
cause kidney damage]. Like lead, it is also excreted via the hair. Lead can
severely affect the nervous system and other biological pathways, but this
isn't seen with uranium [it's damage to kidneys being it's main toxic
effect, although authors of recent DU studies have suggested links to birth
defects and there's the long running controversy over gulf war syndrome]. It
seems that humans have to ingest 10s of grams of natural uranium before
adverse effects are seen in the short term. Animal studies suggest far lower
limits are a wise precaution though with this toxic heavy metal, as damage
has been seen in other organs [e.g. in the lung after inhalation of
'insoluble' enriched UO2 particles]. Natural uranium can deposit on the
bone, but the weak alpha-radiation dose is far too low to induce things like
leukaemia [in all probability]. So it is probably safer to be shot with a
depleted uranium bullet than a lead bullet [but both are best avoided].
Likewise lead shielding is probably potentially more toxic than the uranyl
salts it would be shielding. That's not to say uranium isn't very toxic,
just that soluble lead is even more toxic [hence it's ability to destroy the
Roman Empire from within].

Pelco [who supply the uranyl EM stain] state in there safety sheet that a
material must have a specific activity greater than 74 Becquerel per gram
(Bq/g) in order to be regarded as a radioactive material. One bequeral is
one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted
Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl
acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g],
although the biological hazard from far lower gamma/beta emission rate can
often be considered negligible compared to background or the alpha particle
emission should it become internalised in someone.

So I'd treat uranium with caution as a toxic heavy metal chemical rather
than a radioactive one [as that’s probably its greatest hazard]. Either way
it's hazard is by ingestion or inhalation, so handle with care as outlined
on the supplied safety sheet. External irradiation by the gamma & beta rays
if fairly insignificant from depleted uranium EM stains, and generally most
recommend simply storing in a metal can. It's daughter decay product: Radon
gas, can cause problems in enclosed areas where uranium is abundant in the
soil though.

Regards

Keith J Morris

*U-235 is an interesting isotope and as well as being fissile it can be
measured in extremely minute [trace] quantities using a technique known as
delayed neutron analysis.

http://www.cstl.nist.gov/projects/fy06/fhls0683903.pdf


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone:Â +44 (0)1865 287568
Email:Â kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com]
Sent: 09 January 2009 00:27
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: abesenyo-at-ibilabs.com
Name: Alex Besenyo PhD

Organization: ibilabs

Title-Subject: [Filtered] uranyl compounds are alpha emitters only

Question: Question:

Is it true that the stuff we use has been somehow
depleted, so that it isn't as radioactive as "real" uranyl
salts? Or is this yet another old wive's tale of EM?!

Reply:

When we manufacture these compounds we purchase the raw uranium in a
depleted state from the government. There is no chance for error
here. We do not use natural uranium.

This means that the enrichable uranium U-235 has been removed.
The then U-238 which only emitts alpha radiation is procesed.

The term "depleted" means that U-235 has been removed.

If even by the slightest chance that U235 were present then every
alarm would go off in our facility because Beta and Gamma radiation
is detected.

I hope this answers everybodies concerns.

Our products are sold exclusively through a distributor network and
all of them have been instructed on this information.

I only responded when I saw the original post and I had to respond
before it got out of control.

Sincerely
Alex Besenyo PhD


 Login Host: 74.173.69.139
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009
17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov
[130.202.238.72])
17, 11 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n090Jp6S032218
17, 11 -- Â Â Â for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52
-0600
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]}
17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
39, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jan 13 04:05:07 2009
39, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
39, 23 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DA56tP012959
39, 23 -- Â Â Â for {Microscopy-at-Microscopy.Com} ; Tue, 13 Jan 2009 04:05:06 -0600
39, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
39, 23 -- Â Â Â by morse.well.ox.ac.uk with esmtp (Exim 4.52)
39, 23 -- Â Â Â id 1LMg8v-0006zz-ST
39, 23 -- Â Â Â for Microscopy-at-Microscopy.Com; Tue, 13 Jan 2009 10:05:06 +0000
39, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
39, 23 -- To: {Microscopy-at-Microscopy.Com}
39, 23 -- References: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- Subject: RE: [Microscopy] viaWWW:Yes uranyl compounds do emit few gammas, betas as well
39, 23 -- Date: Tue, 13 Jan 2009 10:05:06 -0000
39, 23 -- Message-ID: {08E534FB0C6245979400D75365C7BAAF-at-CytoWhizz}
39, 23 -- MIME-Version: 1.0
39, 23 -- Content-Type: text/plain;
39, 23 -- Â Â Â charset="iso-8859-1"
39, 23 -- X-Mailer: Microsoft Office Outlook 11
39, 23 -- Thread-Index: Aclx8QYXX4MpJfSGTamN32m5ifY3bwAVUa0g
39, 23 -- In-Reply-To: {200901090027.n090RFFt021207-at-ns.microscopy.com}
39, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
39, 23 -- Content-Transfer-Encoding: 8bit
39, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DA56tP012959
==============================End of - Headers==============================






==============================Original Headers==============================
57, 22 -- From nizets2-at-yahoo.com Tue Jan 13 07:11:23 2009
57, 22 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239])
57, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0DDBMqm009455
57, 22 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 07:11:22 -0600
57, 22 -- Received: (qmail 20923 invoked by uid 60001); 13 Jan 2009 13:11:22 -0000
57, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
57, 22 -- s=s1024; d=yahoo.com;
57, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
57, 22 -- b=AkrMz6GOq46CXKK5pOahDO6ZAfPPx48th9zUS4KULg0103NI1ifS/Zu4WQcIq9wOWLKYF3OO1YZ0dt85zbmE8FcB9bLkv6pP9Hlqm4LU3TxczJUrh6vJ831tTsMFJTsVcPu3d3VnEVudGcCV46iJvZ4S2VZXnhaGLhsxvsG0Tb0=;
57, 22 -- X-YMail-OSG: Zk.PogUVM1kVvQOYpqeXXnlFegUuBHQSs4XK0E87Nd8rz9zPBGBDGggYS18nRO3I5qe6z1y4aJX8L.3g7JMsH0hfefy8cMoX5gdFUcEdg_IpPdXzZl8e9M0hRcD65TsRPyAVZGRvqSOU_m7no6fnPXvwOLR1U7o578vTTjlpHMGglKoYzYIdJ7UAMwm7jQChIxQJCS8dDKLBbKv4ru.Vo4qr7pLEJ.jK
57, 22 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Tue, 13 Jan 2009 05:11:22 PST
57, 22 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1
57, 22 -- References: {200901131012.n0DACTSC020042-at-ns.microscopy.com}
57, 22 -- Date: Tue, 13 Jan 2009 05:11:22 -0800 (PST)
57, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
57, 22 -- Subject: uranyl compounds do emit few gammas, betas as well - Who cares?
57, 22 -- To: microscopy-at-microscopy.com
57, 22 -- MIME-Version: 1.0
57, 22 -- Content-Type: text/plain; charset=utf-8
57, 22 -- Message-ID: {380151.20813.qm-at-web110816.mail.gq1.yahoo.com}
57, 22 -- Content-Transfer-Encoding: 8bit
57, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DDBMqm009455
==============================End of - Headers==============================



==============================Original Headers==============================
69, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jan 13 11:40:13 2009
69, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
69, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DHeCEd031952
69, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 Jan 2009 11:40:12 -0600
69, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
69, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
69, 23 -- id 1LMnFL-0001QN-MX
69, 23 -- for Microscopy-at-Microscopy.Com; Tue, 13 Jan 2009 17:40:11 +0000
69, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
69, 23 -- To: {Microscopy-at-Microscopy.Com}
69, 23 -- References: {200901131318.n0DDIqwG020511-at-ns.microscopy.com}
69, 23 -- Subject: RE: [Microscopy] uranyl compounds do emit few gammas, betas as well - Who cares?
69, 23 -- Date: Tue, 13 Jan 2009 17:40:11 -0000
69, 23 -- Message-ID: {24B91C2D169C48E398B6659F22C2B170-at-CytoWhizz}
69, 23 -- MIME-Version: 1.0
69, 23 -- Content-Type: text/plain;
69, 23 -- charset="utf-8"
69, 23 -- X-Mailer: Microsoft Office Outlook 11
69, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
69, 23 -- Thread-Index: Acl1gXwYNaXi6L9KTy6z4qjW/JomrQAID5Ow
69, 23 -- In-Reply-To: {200901131318.n0DDIqwG020511-at-ns.microscopy.com}
69, 23 -- Content-Transfer-Encoding: 8bit
69, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DHeCEd031952
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Tue, 13 Jan 2009 13:11:45 -0600
Subject: [Microscopy] Re: TEM: sectioning sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Stephane,

They tried examination by SEM but needed better
resolution than we could achieve with our
conventional, older SEMs. I considered using HF
to digest the sand grain but they nixed the idea
since they wanted to show orientation of tubules
relative to the surface. Oh, well.

We did get sections using a diamond knife but the
nanotubes appeared to be round globes rather than
tubes. I'm uncertain if the embedding somehow
messed them up or, more probably, nanotubes had
not formed and we were looking at sphreoidal
materials instead.

John

} Hi John! Sorry for the very late reply but I was
} in well-earned holidays (and yes this year they
} were long). I often cut hard particles under 1
} µm in size with little problem to the knife. It
} is true though that the particles move in the
} resin, leaving holes. I suppose that from a
} given size the damage to the knife becomes a
} real problem. Your particles are probably much
} bigger, which may be a big deal to cut. Now my
} personal opinion: I wonder why TEM would be more
} appropriate than SEM to study the distribution
} of nanotubes at the surface of sand particles.
} And finally, my usual crazy idea: why not try to
} "digest" the sand, leaving only the nanotubes?
} You cannot analyse the interface between
} nanotubes and sand anymore, but if the nanotubes
} are dense enough their organization may be
} conserved. It is better than nothing! Let's
} suppose that the nanotubes are made of carbon,
} they are probably inert to any treatment.
} Following my fellow colleagues (chemists and
} geologists), digesting sand is not an easy task
} though. They suggest something like concentrated
} NaOH. Just my 2 cents, not a lot worth. Stéphane
} ----- Original Message ---- From:
} "bozzola-at-siu.edu" {bozzola-at-siu.edu} To:
} nizets2-at-yahoo.com Sent: Saturday, December 20,
} 2008 12:22:53 AM Subject: [Microscopy] TEM:
} sectioning sand
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America To
} Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} I've cut some hard specimens over the years but
} never sand. We have a researcher who wishes to
} look at a section of a sand grain to study the
} distribution of nanotubes on the surface. Any
} suggestions on sectioning a grain of sand?
} Here's what I am planning: embedding in hard
} Spurr resin old, 50 degree diamond knife 2
} mm/sec cutting speed Thanks, JB --
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} John J. Bozzola, Ph.D., Director Integrated
} Microscopy & Graphics Expertise (IMAGE) Southern
} Illinois University 750 Communications Drive -
} MC 4402 Carbondale, IL 62901 Telephone:
} 618-453-3730
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} ==============================Original
} Headers============================== 8, 17 --
} From bozzola-at-siu.edu Fri Dec 19 17:19:43 2008 8,
} 17 -- Received: from cstmta3.siu.edu
} (cstmta3.siu.edu [131.230.1.3]) 8, 17 -- by
} ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id mBJNJhJX008553 8, 17 -- for
} {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008
} 17:19:43 -0600 8, 17 -- Received: from
} [131.230.177.136] (ws177136.microscope.siu.edu
} [131.230.177.136]) 8, 17 -- by
} cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with
} ESMTP id mBJNJe5s027174 8, 17 -- for
} {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008
} 17:19:42 -0600 (CST) 8, 17 -- Mime-Version: 1.0
} 8, 17 -- Message-Id:
} {a06240812c571db4cd407-at-[131.230.177.136]} 8, 17
} -- Date: Fri, 19 Dec 2008 17:19:36 -0600 8, 17
} -- To: Microscopy-at-microscopy.com 8, 17 -- From:
} "John J. Bozzola" {bozzola-at-siu.edu} 8, 17 --
} Subject: TEM: sectioning sand 8, 17 --
} Content-Type: text/plain; charset="us-ascii" ;
} format="flowed" 8, 17 -- X-Spam-Score: 0.00% 8,
} 17 -- X-MASF: 0.00% 8, 17 -- X-Whitelist: 0.00%
} ==============================End of -
} Headers==============================


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++


==============================Original Headers==============================
10, 22 -- From bozzola-at-siu.edu Tue Jan 13 13:11:45 2009
10, 22 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DJBiOI017929
10, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 13:11:44 -0600
10, 22 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
10, 22 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n0DJBhjc020077
10, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 13:11:44 -0600 (CST)
10, 22 -- Mime-Version: 1.0
10, 22 -- Message-Id: {a06240808c59296ec1624-at-[131.230.177.136]}
10, 22 -- In-Reply-To: {284186.82394.qm-at-web110801.mail.gq1.yahoo.com}
10, 22 -- References: {200812192322.mBJNMruN015105-at-ns.microscopy.com}
10, 22 -- {284186.82394.qm-at-web110801.mail.gq1.yahoo.com}
10, 22 -- Date: Tue, 13 Jan 2009 13:11:42 -0600
10, 22 -- To: Microscopy-at-microscopy.com
10, 22 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
10, 22 -- Subject: Re: [Microscopy] TEM: sectioning sand
10, 22 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 22 -- X-Spam-Score: 0.00%
10, 22 -- X-MASF: 0.00%
10, 22 -- X-Whitelist: 0.00%
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DJBiOI017929
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 14 Jan 2009 03:23:26 -0600
Subject: [Microscopy] Re: RE: Cac buffer and undergrads - chancy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As already said, we have the possibility to use different buffers, and it is a chance!
Because each one has its advantages and drawbacks and is more suited for one application or the other.
I think that for a class this fact is important to teach.
Cacodylate is dangerous and must be manipulated accordingly, but in the end it must be manipulated just like any other hazardous substance! There is no specific manipulation just for cacodylate alone!
In my opinion (just my opinion), cacodylate is mostly used because of inertia force ;-)
In some labs, there is not way, no argumentation which can change the opinion of the boss: he always used cacodylate and always will.
In my opinion (just my opinion), it would be good if we could forget about cacodylate as a "classical" buffer, especially for students. This way the next generation will be more prompt to use other buffers and perhaps keeps the cacodylate buffer just for special application, but not as part of a "classical" protocol.
In the end, teaching is less about perpetuating the same things forever than about learning the basics to allow improvements (I hope this sentence is grammatically correct).

Stéphane





==============================Original Headers==============================
5, 22 -- From nizets2-at-yahoo.com Wed Jan 14 03:23:26 2009
5, 22 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0E9NQ0F027906
5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 03:23:26 -0600
5, 22 -- Received: (qmail 45666 invoked by uid 60001); 14 Jan 2009 09:23:25 -0000
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=yahoo.com;
5, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
5, 22 -- b=V+D0yzi+QYhmvS7FRIWZPMCwbMurltyT/jqfNFjg79+PojhpWJ5J8fMb6atmCBM3yaMZKl6tiXP3cVhJrs6uka7nleXFyGk4FgygfWDSVLrdm0Ad1XEgNHVzGjM9msx6U219KoXYI39lTHGnFiYOyc/rRqJQHoJrcPtsEV4sBLA=;
5, 22 -- X-YMail-OSG: G.AZbNAVM1nB2XGiCf81LO69HKNHF8FBuKsLYkciMpQMnFcPqdmW9xWRTw9HyG5zaBMfjik1kP49iBfeGhTsai1ir0p2.JMEuW3cjDByKwwJWNU6pWqdQdeqwE.hjFS3G9kvPP4U.EJIf9R88s8TQa5V2j7AK3lji_iBQvoebTF0DoPo2mp2pP1D65OIDQ--
5, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Wed, 14 Jan 2009 01:23:25 PST
5, 22 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1
5, 22 -- References: {200901100431.n0A4Ve2v020832-at-ns.microscopy.com}
5, 22 -- Date: Wed, 14 Jan 2009 01:23:25 -0800 (PST)
5, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 22 -- Subject: Re: [Microscopy] RE: Cac buffer and undergrads - chancy?
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=iso-8859-1
5, 22 -- Message-ID: {819009.45662.qm-at-web110805.mail.gq1.yahoo.com}
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0E9NQ0F027906
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Wed, 14 Jan 2009 08:39:45 -0600
Subject: [Microscopy] Looking for SEM With Cryo-stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a user who needs to use an SEM with a cryo-stage. Should be a
short project needing say a single afternoon (freezing, prep,
imaging). Unfortunately we do not have a cryo-stage on our SEM's.

Does anyone have or know of one for use - hopefully within a day's
drive of Oxford Ohio (Think Cincinnati).

Thanks
Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


==============================Original Headers==============================
4, 29 -- From edelmare-at-muohio.edu Wed Jan 14 08:39:42 2009
4, 29 -- Received: from spamfirewall.muohio.edu (walrus.mcs.muohio.edu [134.53.6.27])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0EEdgml021011
4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 08:39:42 -0600
4, 29 -- X-ASG-Debug-ID: 1231943557-235a00b10000-Dem1zR
4, 29 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi
4, 29 -- Received: from mulnx23.mcs.muohio.edu (localhost [127.0.0.1])
4, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 3590FEEB8536
4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500 (EST)
4, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) by spamfirewall.muohio.edu with ESMTP id uomx3cGMe9BoB8aM for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500 (EST)
4, 29 -- X-ASG-Whitelist: Client
4, 29 -- Received: from [192.168.1.23] ([134.53.14.105])
4, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0EEWZE8005130
4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500
4, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
4, 29 -- To: microscopy-at-Microscopy.com
4, 29 -- Date: Wed, 14 Jan 2009 09:32:35 -0500
4, 29 -- MIME-Version: 1.0
4, 29 -- X-ASG-Orig-Subj: Looking for SEM With Cryo-stage
4, 29 -- Subject: Looking for SEM With Cryo-stage
4, 29 -- Message-ID: {496DB133.30031.2EB3BA04-at-edelmare.muohio.edu}
4, 29 -- Priority: normal
4, 29 -- X-mailer: Pegasus Mail for Windows (4.41)
4, 29 -- Content-type: text/plain; charset=US-ASCII
4, 29 -- Content-transfer-encoding: 7BIT
4, 29 -- Content-description: Mail message body
4, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10]
4, 29 -- X-Barracuda-Start-Time: 1231943558
4, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Wed, 14 Jan 2009 16:58:50 -0600
Subject: [Microscopy] Re: RE: Old film etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Phil,
There are still some analog ancients in our world. I found buyers for a
Durst 1200 4 X 5 but not for a floor model !38. Post the equipment on
Craigslist or equivalent.
Larry

oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Good point, although not necessarily the case. The Art and journalism
} deparments here have gone completely digital. Even strictly art
} photography, the kind that uses high silver content paper and so
} forth. They didn't even want a Durst enlarger.
} Anybody on the list looking for a Durst Laborator enlarger in
} excellent condition? With printing easel, extra lenses, etc.?
}
} Phil
}
} } Folks:
} } Old photo/darkroom supplies, etc., may find a happy home in art schools.
} } I have a young artist friend who is taking a photography class and the
} } instructor is insisting that she start with film in order to understand
} } the fundamentals of photography - particularly for B/W. Without getting
} } into arguments about log/linear behavior, benefits of one over the
} } other, etc., the point is that these folks may be able to utilize film
} } supplies and are likely to be very appreciative of a cheap/free source.
} } They may not have much use for the Type xxx film, but some of the other
} } supplies could be valuable.
} } Best Regards,
} } Bill
} } William A. Heeschen
} } Microscopy, Digital Imaging
} } 1897 Bldg, E-84
} } Dow Chemical
} } Midland, MI 48667
} } mailto:waheeschen-at-dow.com
} }
} } -----Original Message-----
} } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
} } Sent: Monday, January 12, 2009 5:04 PM
} } To: Heeschen, Bill (WA)
} } Subject: [Microscopy] Old film etc
} }
} ---
} } Greetings
} }
} } This is a message I never thought I would be sending.
} }
} } We are getting out of the film and paper photo business, and have a
} } lot of 'stuff' to give away or send to the landfill. Anyone with
} } questions about whether digital imaging for EM is for real, let this
} } be your wake-up call.
} }
} } Most of what we have is old, probably not worth the cost to ship,
} } unless you are desperate, or curious.
} }
} } We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572.
} } I don't even know what some of this was used for, if it rings your
} } bell, let me know and I can tell you more.
} }
} } We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4
} } x5 sizes.
} }
} } A little 4463 and a ton of SO-163. The SO-163 is old.
} }
} } Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.
} }
} } Most of this junk has been in a freezer. Some is pretty old and past
} } its expiration date, but if you are interested, let me know and we can
} } try to work something out. If I don't hear anything in the next week
} } or so, its outa here.
} }
} } Jon
} }
} } Jonathan Krupp
} } Delta College
} } 5151Pacific Ave.
} } Stockton, CA 95207
} } 209-954-5284
} } jkrupp-at-deltacollege.edu
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
6, 39 -- From Larry.Ackerman-at-ucsf.edu Wed Jan 14 16:58:50 2009
6, 39 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98])
6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0EMwn25022667
6, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 16:58:49 -0600
6, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with
6, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
6, 39 -- Wed, 14 Jan 2009 14:58:39 -0800
6, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
6, 39 -- X-AuditID: 403623d2-a5611bb000007fb9-ed-496e8a56654a
6, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org (
6, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by
6, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
6, 39 -- C975430C for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 16:59:02
6, 39 -- -0800 (PST)
6, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
6, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830);
6, 39 -- Wed, 14 Jan 2009 14:58:38 -0800
6, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
6, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Wed, 14 Jan
6, 39 -- 2009 14:58:38 -0800
6, 39 -- Message-ID: {496E6E1E.8060502-at-ucsf.edu}
6, 39 -- Date: Wed, 14 Jan 2009 14:58:38 -0800
6, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
6, 39 -- Reply-to: larry.ackerman-at-ucsf.edu
6, 39 -- Organization: UCSF, NeuroAnatomy
6, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 39 -- MIME-Version: 1.0
6, 39 -- To: Microscopy-at-microscopy.com
6, 39 -- Subject: Re: [Microscopy] RE: Old film etc
6, 39 -- References: {200901131505.n0DF53Nl005349-at-ns.microscopy.com}
6, 39 -- In-Reply-To: {200901131505.n0DF53Nl005349-at-ns.microscopy.com}
6, 39 -- X-OriginalArrivalTime: 14 Jan 2009 22:58:38.0895 (UTC)
6, 39 -- FILETIME=[A353B3F0:01C9769B]
6, 39 -- X-Brightmail-Tracker: AAAAAQ05Q1k=
6, 39 -- X-WSS-ID: 6570B1951OO2346768-01-01
6, 39 -- Content-Type: text/plain;
6, 39 -- charset=iso-8859-1;
6, 39 -- format=flowed
6, 39 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: sam.telford-at-tufts.edu
Date: Thu, 15 Jan 2009 18:32:43 -0600
Subject: [Microscopy] viaWWW: infinity dk series digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sam.telford-at-tufts.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sam.telford-at-tufts.edu
Name: sam telford

Organization: tufts university

Title-Subject: [Filtered] infinity dk series digital camera

Question:
Does anyone have any experience with the Infinity DK Series -- Meiji
makes this, I think -- digital setup (particularly the new 5.1 MP
camera) for capturing images from a compound scope? I am
particularly interested in reliability, ease of use, and quality of
images. Are there other setups I should think about for the same
price ($3400)? This would be used for publication quality
documentation of blood smears (X250-X1000) or of histopathology
material.

Login Host: 130.64.245.192
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jan 15 18:32:42 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0G0WfMx004943
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2009 18:32:42 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c595861916d0-at-[206.69.208.22]}
6, 11 -- Date: Thu, 15 Jan 2009 18:32:40 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: sam.telford-at-tufts.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: infinity dk series digital camera
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: radhika_aaryan-at-yahoo.co.in
Date: Thu, 15 Jan 2009 18:33:12 -0600
Subject: [Microscopy] viaWWW: regarding scanning electron microscopic imaging of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both radhika_aaryan-at-yahoo.co.in as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: radhika_aaryan-at-yahoo.co.in
Name: radhika gupta

Organization: Vokkaligarh Sangha Dental College

Title-Subject: [Filtered] regarding scanning electron microscopic
imaging of extracted human teeth

Question: how do i differentiate between the cementum and dentine of
extracted human teeth in the micrographs obtained from scanning
electron microscope? is the cementodentinal structure clearly
demarcated? does cementum have any characteristic apperance when seen
under scanning electro microscope?

Login Host: 220.226.72.23
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Thu Jan 15 18:33:12 2009
6, 12 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0G0XAA1005417
6, 12 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2009 18:33:11 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- Message-Id: {p06240801c59586351d59-at-[206.69.208.22]}
6, 12 -- Date: Thu, 15 Jan 2009 18:33:09 -0600
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: radhika_aaryan-at-yahoo.co.in (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: regarding scanning electron microscopic imaging of
6, 12 -- extracted human teeth
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: idrucker-at-semitool.com
Date: Thu, 15 Jan 2009 18:33:39 -0600
Subject: [Microscopy] viaWWW: SE/Everhart-Thornley Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both idrucker-at-semitool.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: idrucker-at-semitool.com
Name: Ian Drucker

Organization: Semitool Inc.

Title-Subject: [Filtered] SE/Everhart-Thornley Detectors

Question: We're currently using a CDEM detector on an FEI FIB820. We
use this detector for both our Ion and SEM images. It's one of the
older FEI systems and still uses Windows 3.1 with an outdated PC.

Does anyone know of a company that has a SE detector that would be
compatible with our system or possibly be a standalone detector that
would only need a support PC to run it?

Thanks




Login Host: 207.118.55.15
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Jan 15 18:33:39 2009
11, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0G0XbiT006608
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2009 18:33:38 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240802c595864f238a-at-[206.69.208.22]}
11, 11 -- Date: Thu, 15 Jan 2009 18:33:36 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: idrucker-at-semitool.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: SE/Everhart-Thornley Detectors
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: pveril-at-med.uth.gr
Date: Fri, 16 Jan 2009 08:06:50 -0600
Subject: [Microscopy] viaWWW: Looking for a used TEM or SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pveril-at-med.uth.gr as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pveril-at-med.uth.gr
Name: Panagiotis Berillis

Organization: University of Thessaly, Greece

Title-Subject: [Filtered] Looking for a used TEM or SEM

Question: Hi,my department is looking to buy a used but working SEM
or TEM. Please contact me for any information. Thank you.

Login Host: 195.251.17.162
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Jan 16 08:06:49 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GE6kMV009363
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 08:06:48 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c59644e0ce76-at-[206.69.208.22]}
6, 11 -- Date: Fri, 16 Jan 2009 08:06:45 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: pveril-at-med.uth.gr (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Looking for a used TEM or SEM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jsiegmund-at-7thwavelabs.com
Date: Fri, 16 Jan 2009 08:50:21 -0600
Subject: [Microscopy] viaWWW: infinity dk series digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi All,
We have mostly Spot cameras and Olympus cameras in our setups.
Our Pathologists really like the easy to use Spot cameras.
Very intuitive software and wonderful images.




-----Original Message-----
X-from: sam.telford-at-tufts.edu [mailto:sam.telford-at-tufts.edu]
Sent: Thursday, January 15, 2009 6:50 PM
To: Joachim Siegmund

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both sam.telford-at-tufts.edu as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: sam.telford-at-tufts.edu
Name: sam telford

Organization: tufts university

Title-Subject: [Filtered] infinity dk series digital camera

Question:
Does anyone have any experience with the Infinity DK Series -- Meiji
makes this, I think -- digital setup (particularly the new 5.1 MP
camera) for capturing images from a compound scope? I am
particularly interested in reliability, ease of use, and quality of
images. Are there other setups I should think about for the same
price ($3400)? This would be used for publication quality
documentation of blood smears (X250-X1000) or of histopathology
material.

Login Host: 130.64.245.192
------------------------------------------------------------------------
---

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jan 15 18:32:42 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov
[130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0G0WfMx004943
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2009
18:32:42 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c595861916d0-at-[206.69.208.22]}
6, 11 -- Date: Thu, 15 Jan 2009 18:32:40 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: sam.telford-at-tufts.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: infinity dk series digital camera
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================

This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer


==============================Original Headers==============================
19, 29 -- From jsiegmund-at-7thwavelabs.com Fri Jan 16 08:50:21 2009
19, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136])
19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GEoLlH024425
19, 29 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 08:50:21 -0600
19, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1])
19, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id C7A2839800A;
19, 29 -- Fri, 16 Jan 2009 08:50:20 -0600 (CST)
19, 29 -- X-AuditID: c0a80218-a7843bb000000bbe-8f-49709eac5030
19, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29])
19, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 5C363424013;
19, 29 -- Fri, 16 Jan 2009 08:50:20 -0600 (CST)
19, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 29 -- Content-class: urn:content-classes:message
19, 29 -- MIME-Version: 1.0
19, 29 -- Content-Type: text/plain;
19, 29 -- charset="us-ascii"
19, 29 -- Subject: RE: [Microscopy] viaWWW: infinity dk series digital camera
19, 29 -- Date: Fri, 16 Jan 2009 08:50:19 -0600
19, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A658B5B4-at-wave-mail.7thwave.local}
19, 29 -- X-MS-Has-Attach:
19, 29 -- X-MS-TNEF-Correlator:
19, 29 -- Thread-Topic: [Microscopy] viaWWW: infinity dk series digital camera
19, 29 -- Thread-Index: Acl3dExx2JOtrkwNQluVccJslXOnVQAdITwg
19, 29 -- References: {200901160049.n0G0nWLF013465-at-ns.microscopy.com}
19, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com}
19, 29 -- To: {sam.telford-at-tufts.edu} , {microscopy-at-microscopy.com}
19, 29 -- X-Brightmail-Tracker: AAAAAg04csgNOUNZ
19, 29 -- Content-Transfer-Encoding: 8bit
19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0GEoLlH024425
==============================End of - Headers==============================




From: waldenzz-at-gmail.com
Date: Fri, 16 Jan 2009 13:47:59 -0600
Subject: [Microscopy] viaWWW: Mount a dslr on BX51

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both waldenzz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: waldenzz-at-gmail.com
Name: Jonathan Zhang

Organization: University of Washington

Title-Subject: [Filtered] Mount a dslr on BX51

Question:

We are users of an Olympus BX51 Microscope and trying to find a
simple solution to take microscopic pictures. Since the microscope
has a C mount, could we just mount a C-mount adapter and connect it
to a 35 mm SLR camera (like a Nikon D40)?

Thank you very much for your help,

Jonathan


Login Host: 38.97.76.4
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Fri Jan 16 13:47:59 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GJlw9B017233
10, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 13:47:59 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c59694cf8ae2-at-[206.69.208.22]}
10, 11 -- Date: Fri, 16 Jan 2009 13:47:57 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: waldenzz-at-gmail.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Mount a dslr on BX51
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: qxing-at-ameslab.gov
Date: Fri, 16 Jan 2009 15:34:33 -0600
Subject: [Microscopy] viaWWW: X-ray Back Laue collimation system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both qxing-at-ameslab.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: qxing-at-ameslab.gov
Name: Qingfeng Xing

Organization: Ames Laboratory

Title-Subject: [Filtered] X-ray Back Laue collimation system

Question: Dear colleagues:

Does anyone know the requirements for designing a collimation system
for higher accuracy in back Laue X-ray diffraction?

It seems that the geometry is simple. Are there any tricks?

Thank you
Qingfeng

Login Host: 147.155.80.87
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri Jan 16 15:34:33 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GLYWrZ002328
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 15:34:32 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c596adcb660e-at-[206.69.208.22]}
9, 11 -- Date: Fri, 16 Jan 2009 15:34:31 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: qxing-at-ameslab.gov (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: X-ray Back Laue collimation system
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Williams-at-GENECTR.HUNTER.CUNY.EDU
Date: Fri, 16 Jan 2009 16:48:33 -0600
Subject: [Microscopy] Camera and Software Suggestion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for a suggestion for a video camera to attach to a C
mount on a disecting microscope, that can be used to make AVI or MPEG
movies. Also for some simple movie making software, along the lines of
iMovie but for a PC. The idea being to create Avi movies of lab
protocols
Thanks in advance
Lloyd Williams

Sent from my iPhone


==============================Original Headers==============================
3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009
3, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GMmVfW018101
3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33 -0600
3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by
3, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id
3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500
3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by
3, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009
3, 26 -- 17:48:50 -0500
3, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU}
3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
3, 26 -- Date: Fri, 16 Jan 2009 17:48:23 -0500
3, 26 -- Subject: Camera and Software Suggestion
3, 26 -- Thread-Topic: Camera and Software Suggestion
3, 26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g==
3, 26 -- Message-ID: {65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu}
3, 26 -- Accept-Language: en-US
3, 26 -- Content-Language: en-US
3, 26 -- X-MS-Has-Attach:
3, 26 -- X-MS-TNEF-Correlator:
3, 26 -- acceptlanguage: en-US
3, 26 -- Content-Type: text/plain; charset="us-ascii"
3, 26 -- MIME-Version: 1.0
3, 26 -- Content-Transfer-Encoding: 8bit
3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101
==============================End of - Headers==============================




From: hanke-at-mee-inc.com
Date: Fri, 16 Jan 2009 17:07:38 -0600
Subject: [Microscopy] RE: polishing stainless steel and silver metal surfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Previous advice on this topic from Alan and Jeff was excellent. I have a
couple of other caveats that you may want to consider.

One other note is that grain size measurement for these materials in
most forms will be much easier with light microscopy than with SEM.
Subtle topography created by the etching is often difficult to image by
SEM, but readily observed by LM. You will probably not need
magnification greater than about 500X unless you have something like
very fine wire or thin sheet materials. We do grain size with SEM on
stainless steels with very fine grains, but sample preparation and
imaging are critical for accurate results.

Secondly, if the material is in a cold worked condition, it will be very
difficult to see the individual grains. If you are not familiar with the
techniques and structures, you could spend days working on cold-worked
stainless steel thinking that your technique was bad when the actual
microstructure just does not have distinctly delineated grains.
Likewise, some stainless steels have a martensitic structure that may
not exhibit distinct grain boundaries or the grain boundaries may be
difficult to recognize without experience.

Sorry to chime in late on this topic, but thought that this might be
helpful if you haven't got it all figured out already.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

} Listers - I have samples to prepare for inspection by SEM, and the client
} wants to know about the grain size. We have never polished metals before.
} Is there anyone who can advise me what to do, or does this take a
} metallurgist? Carol Heckman, Bowling Green State University


==============================Original Headers==============================
7, 22 -- From hanke-at-mee-inc.com Fri Jan 16 17:07:38 2009
7, 22 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GN7cW8032270
7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 17:07:38 -0600
7, 22 -- Received: (qmail 9846 invoked by uid 508); 16 Jan 2009 17:07:38 -0600
7, 22 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms
7, 22 -- (perlscan: 1.24-st-qms.
7, 22 -- Clear:RC:1(216.14.180.82):.
7, 22 -- Processed in 0.031652 secs); 16 Jan 2009 23:07:38 -0000
7, 22 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com
7, 22 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.031652 secs Process 9841)
7, 22 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82)
7, 22 -- by mail.namisolutions.com with SMTP; 16 Jan 2009 17:07:37 -0600
7, 22 -- Message-ID: {49711337.309-at-mee-inc.com}
7, 22 -- Date: Fri, 16 Jan 2009 17:07:35 -0600
7, 22 -- From: Larry Hanke {hanke-at-mee-inc.com}
7, 22 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: heckman-at-buckeye-express.com, microscopy-at-microscopy.com
7, 22 -- Subject: RE: polishing stainless steel and silver metal surfaces
7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: arnec-at-bio.umass.edu
Date: Sat, 17 Jan 2009 14:42:49 -0600
Subject: [Microscopy] viaWWW: Methods to deglycosylate fixed tissue sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For a really inexpensive approach, I used a Qsee digital surveillance
camera, model QSPSC, that I bought from Fry's electronics that cost about
$70. I took the lens off and it was a C-mount and hooked it up to the
microscope. I took the video out and put it into my video camera and
recorded the image. I then could make a DVD from it. I did this for
essentially the same reason that you are trying to do. The quality good
enough for showing people what you are trying to do under the microscope.
I've hooked this up to a DVD player and a monitor with surprising results.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU
[mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU]
Sent: Friday, January 16, 2009 2:52 PM
To: Walck-at-SouthBayTech.com

I am looking for a suggestion for a video camera to attach to a C mount on a
disecting microscope, that can be used to make AVI or MPEG movies. Also for
some simple movie making software, along the lines of iMovie but for a PC.
The idea being to create Avi movies of lab protocols Thanks in advance Lloyd
Williams

Sent from my iPhone


==============================Original Headers==============================
3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009 3,
26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu
[146.95.150.34])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0GMmVfW018101
3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33
-0600
3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 3,
26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server
(TLS) id 3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500 3, 26 --
Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 3, 26 --
Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009
3, 26 -- 17:48:50 -0500 3, 26 -- From: Lloyd Williams
{Williams-at-GENECTR.HUNTER.CUNY.EDU}
3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 26
-- Date: Fri, 16 Jan 2009 17:48:23 -0500 3, 26 -- Subject: Camera and
Software Suggestion 3, 26 -- Thread-Topic: Camera and Software Suggestion 3,
26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g== 3, 26 -- Message-ID:
{65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu}
3, 26 -- Accept-Language: en-US
3, 26 -- Content-Language: en-US
3, 26 -- X-MS-Has-Attach:
3, 26 -- X-MS-TNEF-Correlator:
3, 26 -- acceptlanguage: en-US
3, 26 -- Content-Type: text/plain; charset="us-ascii"
3, 26 -- MIME-Version: 1.0
3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from
quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101
==============================End of - Headers==============================

Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
5:29 PM


==============================Original Headers==============================
13, 22 -- From walck-at-southbaytech.com Fri Jan 16 21:18:14 2009
13, 22 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82])
13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0H3IEqQ019522
13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 21:18:14 -0600
13, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201])
13, 22 -- (authenticated bits=0)
13, 22 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0H3IAmH029444;
13, 22 -- Fri, 16 Jan 2009 21:18:11 -0600
13, 22 -- From: "Scott Walck" {walck-at-southbaytech.com}
13, 22 -- To: {Microscopy-at-microscopy.com}
13, 22 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU}
13, 22 -- Subject: RE: [Microscopy] Camera and Software Suggestion
13, 22 -- Date: Fri, 16 Jan 2009 19:18:30 -0800
13, 22 -- Message-ID: {8F9BA85574E9478F8728000632C5E4B8-at-dynamicbl8uno3}
13, 22 -- MIME-Version: 1.0
13, 22 -- Content-Type: text/plain;
13, 22 -- charset="us-ascii"
13, 22 -- Content-Transfer-Encoding: 7bit
13, 22 -- X-Mailer: Microsoft Office Outlook 11
13, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
13, 22 -- Thread-Index: Acl4LQY4wMt32pgzQAeGPPImp8S+DQAI7dKg
13, 22 -- In-Reply-To: {200901162251.n0GMplGZ023119-at-ns.microscopy.com}
==============================End of - Headers==============================

From nicgugliucci-at-hotmail.com Sat Jan 17 04:22:13 2009
Return-Path: {nicgugliucci-at-hotmail.com}
Received: from google.com (122.2.169.154.pldt.net [122.2.169.154] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0HAMB50019014
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 17 Jan 2009 04:22:12 -0600
Received: from [148.134.79.59] (HELO google.com)
by gladlay.com; Sat, 17 Jan 2009 18:33:57 -0800
Message-ID: {0000000653A18321278805761}
Reply-To: Allyn Losey {kimgigbank-at-yahoo.com.sg}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: arnec-at-bio.umass.edu
Name: Arne

Title-Subject: [Filtered] Methods to deglycosylate fixed tissue sections

Question: I've been having trouble with an antibody directed against
an epitope with a putative gycosylation site. I'm wondering if
anybody can recommend a preferred method for deglycosylating
(N-linked) proteins in fixed tissue sections. I'm particularly
interested in enzymatic deglycosylation with PNGase F.

Login Host: 159.189.36.78
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Sat Jan 17 14:42:49 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0HKglOY024125
5, 11 -- for {microscopy-at-microscopy.com} ; Sat, 17 Jan 2009 14:42:49 -0600
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240807c597f338aced-at-[206.69.208.22]}
5, 11 -- Date: Sat, 17 Jan 2009 14:42:46 -0600
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: arnec-at-bio.umass.edu (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Methods to deglycosylate fixed tissue sections
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Hobie-at-technicalsalessolutions.com
Date: Sun, 18 Jan 2009 20:23:47 -0600
Subject: [Microscopy] Boekel Digital Incubator ~ Free!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

We still have a Boekel table top digital incubator that needs to find a
home. ~ It is a brand new unit in its original shipping box.

Please contact me for pictures and specs. It will only cost you the
shipping. If you want to make a donation for it to Valley Catholic HS EM
Lab that's ok too.

Thank you,

Hobie

Hobie Richards
Partner, and COO
Technical Sales Solutions, LLC
Portland, OR USA
www.TechnicalSalesSolutions.com
503 781 0428

Skype Hobie-TSS




==============================Original Headers==============================
10, 19 -- From Hobie-at-technicalsalessolutions.com Sun Jan 18 20:23:47 2009
10, 19 -- Received: from host203.com (host203.com [203.194.159.243])
10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0J2Nh1E031231
10, 19 -- for {microscopy-at-microscopy.com} ; Sun, 18 Jan 2009 20:23:45 -0600
10, 19 -- Received: (qmail 1883 invoked by uid 503); 19 Jan 2009 02:23:40 -0000
10, 19 -- Received: from unknown (HELO ?10.0.1.195?) (Hobie-at-76.115.10.232)
10, 19 -- by host203.com with ESMTPA; 19 Jan 2009 02:23:40 -0000
10, 19 -- User-Agent: Microsoft-Entourage/12.0.0.071130
10, 19 -- Date: Sun, 18 Jan 2009 18:23:34 -0800
10, 19 -- Subject: Boekel Digital Incubator ~ Free!
10, 19 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com}
10, 19 -- To: {microscopy-at-microscopy.com}
10, 19 -- Message-ID: {C5992426.16576%Hobie-at-technicalsalessolutions.com}
10, 19 -- Thread-Topic: Boekel Digital Incubator ~ Free!
10, 19 -- Thread-Index: Acl53O1uKv/DV2wDY06DKy6vVaHJXQ==
10, 19 -- Mime-version: 1.0
10, 19 -- Content-type: text/plain;
10, 19 -- charset="US-ASCII"
10, 19 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Mon, 19 Jan 2009 10:24:36 -0600
Subject: [Microscopy] Short Course Announcement: SEM and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Fellow Microscopists,

The College of Microscopy, located in Westmont, IL, is offering the following electron microscopy short courses:

March 16 to 20, 2009 - Scanning Electron Microscopy

March 24 to 26, 2009 - Transmission Electron Microscopy

In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.

www.collegeofmicroscopy.com

Regards,

Elaine

********************************************************************* 
Elaine F. Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL  60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail:      eschumacher-at-mccrone.com
Web Site:  www.mccrone.com




==============================Original Headers==============================
12, 23 -- From eschumacher-at-mccrone.com Mon Jan 19 10:24:36 2009
12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0JGOZfR022953
12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 10:24:36 -0600
12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959);
12, 23 -- Mon, 19 Jan 2009 10:24:06 -0600
12, 23 -- Content-class: urn:content-classes:message
12, 23 -- MIME-Version: 1.0
12, 23 -- Content-Type: text/plain;
12, 23 -- charset="iso-8859-1"
12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 23 -- Subject: Short Course Announcement: SEM and TEM
12, 23 -- Date: Mon, 19 Jan 2009 10:24:05 -0600
12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A701EBB792-at-MCCRONEMSG.tmg.mccrone.com}
12, 23 -- X-MS-Has-Attach:
12, 23 -- X-MS-TNEF-Correlator:
12, 23 -- Thread-Topic: Short Course Announcement: SEM and TEM
12, 23 -- Thread-Index: Acl6Ulj6k6nl7JoySoeTuoktHBriPQ==
12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
12, 23 -- To: {microscopy-at-microscopy.com}
12, 23 -- X-OriginalArrivalTime: 19 Jan 2009 16:24:06.0285 (UTC) FILETIME=[596AD3D0:01C97A52]
12, 23 -- Content-Transfer-Encoding: 8bit
12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0JGOZfR022953
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Mon, 19 Jan 2009 13:42:31 -0600
Subject: [Microscopy] RE: Camera and Software Suggestion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lloyd:

Scott's camera suggestion may be a good one. But rather than the
runnin through another video camera, I would suggest one of the
simple USB video capture devices. I got a "fancy" Diamond
multimedia VC500 a couple of months ago to do similar (Already had
older c-mount video cameras), and it works great. "Fancy" means it
does both composite and s-video, NTSC, PAL, Etc. It cost $35 at
amazon.

Since your at CUNY you might just hit some of the camera stores in
The City and see what they may have in c-mount video camera's.





On 16 Jan 2009 at 22:19, walck-at-southbaytech.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} For a really inexpensive approach, I used a Qsee digital surveillance
} camera, model QSPSC, that I bought from Fry's electronics that cost about
} $70. I took the lens off and it was a C-mount and hooked it up to the
} microscope. I took the video out and put it into my video camera and
} recorded the image. I then could make a DVD from it. I did this for
} essentially the same reason that you are trying to do. The quality good
} enough for showing people what you are trying to do under the microscope.
} I've hooked this up to a DVD player and a monitor with surprising results.
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} Technical Director
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} US Toll Free: 1-800-728-2233
} Tel: (949) 492-2600
} Fax: (949) 492-1499
}
} www.southbaytech.com
} walck-at-southbaytech.com
}
} -----Original Message-----
} X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU
} [mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU]
} Sent: Friday, January 16, 2009 2:52 PM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] Camera and Software Suggestion
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I am looking for a suggestion for a video camera to attach to a C mount on a
} disecting microscope, that can be used to make AVI or MPEG movies. Also for
} some simple movie making software, along the lines of iMovie but for a PC.
} The idea being to create Avi movies of lab protocols Thanks in advance Lloyd
} Williams
}
} Sent from my iPhone
}
}
} ==============================Original Headers==============================
} 3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009 3,
} 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu
} [146.95.150.34])
} 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0GMmVfW018101
} 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33
} -0600
} 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 3,
} 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server
} (TLS) id 3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500 3, 26 --
} Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 3, 26 --
} Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009
} 3, 26 -- 17:48:50 -0500 3, 26 -- From: Lloyd Williams
} {Williams-at-GENECTR.HUNTER.CUNY.EDU}
} 3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 26
} -- Date: Fri, 16 Jan 2009 17:48:23 -0500 3, 26 -- Subject: Camera and
} Software Suggestion 3, 26 -- Thread-Topic: Camera and Software Suggestion 3,
} 26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g== 3, 26 -- Message-ID:
} {65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu}
} 3, 26 -- Accept-Language: en-US
} 3, 26 -- Content-Language: en-US
} 3, 26 -- X-MS-Has-Attach:
} 3, 26 -- X-MS-TNEF-Correlator:
} 3, 26 -- acceptlanguage: en-US
} 3, 26 -- Content-Type: text/plain; charset="us-ascii"
} 3, 26 -- MIME-Version: 1.0
} 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from
} quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101
} ==============================End of - Headers==============================
}
} Internal Virus Database is out of date.
} Checked by AVG - http://www.avg.com
} Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
} 5:29 PM
}
}
} ==============================Original Headers==============================
} 13, 22 -- From walck-at-southbaytech.com Fri Jan 16 21:18:14 2009
} 13, 22 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82])
} 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0H3IEqQ019522
} 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 21:18:14 -0600
} 13, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201])
} 13, 22 -- (authenticated bits=0)
} 13, 22 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0H3IAmH029444;
} 13, 22 -- Fri, 16 Jan 2009 21:18:11 -0600
} 13, 22 -- From: "Scott Walck" {walck-at-southbaytech.com}
} 13, 22 -- To: {Microscopy-at-microscopy.com}
} 13, 22 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU}
} 13, 22 -- Subject: RE: [Microscopy] Camera and Software Suggestion
} 13, 22 -- Date: Fri, 16 Jan 2009 19:18:30 -0800
} 13, 22 -- Message-ID: {8F9BA85574E9478F8728000632C5E4B8-at-dynamicbl8uno3}
} 13, 22 -- MIME-Version: 1.0
} 13, 22 -- Content-Type: text/plain;
} 13, 22 -- charset="us-ascii"
} 13, 22 -- Content-Transfer-Encoding: 7bit
} 13, 22 -- X-Mailer: Microsoft Office Outlook 11
} 13, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 13, 22 -- Thread-Index: Acl4LQY4wMt32pgzQAeGPPImp8S+DQAI7dKg
} 13, 22 -- In-Reply-To: {200901162251.n0GMplGZ023119-at-ns.microscopy.com}
} ==============================End of - Headers==============================


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


==============================Original Headers==============================
13, 24 -- From edelmare-at-muohio.edu Mon Jan 19 13:42:28 2009
13, 24 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70])
13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0JJgSxd011204
13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 13:42:28 -0600
13, 24 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
13, 24 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0JJgU6v018846
13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 14:42:30 -0500
13, 24 -- Received: from [192.168.1.23] ([134.53.14.105])
13, 24 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0JJgRHq029772
13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 14:42:28 -0500
13, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
13, 24 -- To: microscopy-at-Microscopy.com
13, 24 -- Date: Mon, 19 Jan 2009 14:42:27 -0500
13, 24 -- MIME-Version: 1.0
13, 24 -- Subject: [Microscopy] RE: Camera and Software Suggestion
13, 24 -- Message-ID: {49749153.27429.498F08FE-at-edelmare.muohio.edu}
13, 24 -- Priority: normal
13, 24 -- In-reply-to: {200901170319.n0H3JWKH020545-at-ns.microscopy.com}
13, 24 -- References: {200901170319.n0H3JWKH020545-at-ns.microscopy.com}
13, 24 -- X-mailer: Pegasus Mail for Windows (4.41)
13, 24 -- Content-type: text/plain; charset=US-ASCII
13, 24 -- Content-transfer-encoding: 7BIT
13, 24 -- Content-description: Mail message body
13, 24 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Mon, 19 Jan 2009 18:13:06 -0600
Subject: [Microscopy] Calling all former Delta College students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings to all:

I am a new instructor at Delta College in Stockton, California.

I would like to make contact with anyone reading the list who has some
affiliation with Delta.

If you attended Delta in the past, graduated or not, or know someone
who did, could you send me some contact info. I want to make a list of
folks who have left here and are now in the work force. Lots of the
current students are curious about your experience, the kinds of jobs
available, even specifics like hours and pay.

Even if you're not a Delta type, any info about realistic expectations
for our students thinking about jobs, their plans and their future
would be great. What are the skills and qualities that we need to
instill in our students?

As I collect info, I'll put it together in a simple directory for us
to share.

Thanks

Jon


Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
13, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 19 18:13:05 2009
13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0K0CpGT005180
13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 18:13:04 -0600
13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
13, 42 -- by localhost (Email Security Appliance) with SMTP id 5DECD1BA741
13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 23:47:43 +0000 (GMT)
13, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
13, 42 -- by mailin.deltacollege.edu (Email Security Appliance) with ESMTP id 552141689F9
13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 23:47:43 +0000 (GMT)
13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
13, 42 -- with ESMTP id 45104006 for microscopy-at-microscopy.com; Mon, 19 Jan 2009 16:12:45 -0800
13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
13, 42 -- ESMTP id KDQT7A00.UGS for {microscopy-at-microscopy.com} ; Mon, 19
13, 42 -- Jan 2009 15:57:10 -0800
13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 96FF08F74BA5
13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 16:12:45 -0800 (PST)
13, 42 -- X-Virus-Scanned: amavisd-new at
13, 42 -- X-Spam-Flag: NO
13, 42 -- X-Spam-Score: -2.499
13, 42 -- X-Spam-Level:
13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000,
13, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
13, 42 -- with ESMTP id Y1ixOY7peTUu for {microscopy-at-microscopy.com} ;
13, 42 -- Mon, 19 Jan 2009 16:12:41 -0800 (PST)
13, 42 -- Received: from [172.20.8.17] (unknown [172.20.8.17])
13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D59C58F74B26
13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 16:12:41 -0800 (PST)
13, 42 -- Message-Id: {4AAB3392-6686-4E7C-B11B-2019FC8A8513-at-deltacollege.edu}
13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
13, 42 -- To: microscopy-at-microscopy.com
13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 42 -- Content-Transfer-Encoding: 7bit
13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 42 -- Subject: Calling all former Delta College students
13, 42 -- Date: Mon, 19 Jan 2009 16:12:41 -0800
13, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: kamlennon-at-yahoo.com
Date: Tue, 20 Jan 2009 13:35:39 -0600
Subject: [Microscopy] Good fix for bacteria/microorganisms?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

I'm searching for a good chemical fixation protocol for bacteria/microorganisms for an EM class that I'm developing for undergrads. Since the class hasn't been taught at my university for several years (8), it seems that most/all of the microscopy journals have been dropped from the libary. So, if you would share your protocol, I would be ever-grateful.

Many thanks,
Kristen

Kristen A. Lennon, Ph.D.

Lecturer, Department of Biology

202 Compton Science Center

Frostburg State University

101 Braddock Road

Frostburg, MD 21532

301-687-4697

k.lennon-at-frostburg.edu





==============================Original Headers==============================
14, 20 -- From kamlennon-at-yahoo.com Tue Jan 20 13:35:38 2009
14, 20 -- Received: from web84003.mail.mud.yahoo.com (web84003.mail.mud.yahoo.com [68.142.206.173])
14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0KJZab4024990
14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2009 13:35:37 -0600
14, 20 -- Received: (qmail 15715 invoked by uid 60001); 20 Jan 2009 19:35:35 -0000
14, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 20 -- s=s1024; d=yahoo.com;
14, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
14, 20 -- b=3X+spjmYxBHstMM4Bf4f/vJFFwKPOd8u0thFOtWIh0U8jGTzdCIwfJO6e+W8cgypiQ1DZvfRtExVKg3GPrCGfYeSK9qvy2TF21oFvBBRtLwyX6GdFQc3QXykNIVWt2oSqjX/pxu6rn12DSq/AtAgSoAd0R2/Vnf85S4lco7xufI=;
14, 20 -- X-YMail-OSG: x6vDz9AVM1lQIKrJVRdey1aVkpZQbfjUZm0QZ_tNhwf59PsB2ao-
14, 20 -- Received: from [96.239.149.81] by web84003.mail.mud.yahoo.com via HTTP; Tue, 20 Jan 2009 11:35:35 PST
14, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
14, 20 -- Date: Tue, 20 Jan 2009 11:35:35 -0800 (PST)
14, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
14, 20 -- Reply-To: kamlennon-at-yahoo.com
14, 20 -- Subject: Good fix for bacteria/microorganisms?
14, 20 -- To: microscopy-at-microscopy.com
14, 20 -- MIME-Version: 1.0
14, 20 -- Content-Type: text/plain; charset=us-ascii
14, 20 -- Message-ID: {649576.15497.qm-at-web84003.mail.mud.yahoo.com}
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Tue, 20 Jan 2009 14:03:55 -0600
Subject: [Microscopy] Re: Good fix for bacteria/microorganisms?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kristen,

Will your bacteria be negatively stained or will they be fixed inside cells,
like macrophages?

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
connellyps-at-mail.nih.gov
======
} From: {kamlennon-at-yahoo.com}
} Reply-To: {kamlennon-at-yahoo.com}
} Date: Tue, 20 Jan 2009 13:55:56 -0600
} To: {connellyps-at-nhlbi.nih.gov}
} Subject: [Microscopy] Good fix for bacteria/microorganisms?
}
} Hi Listers,
}
} I'm searching for a good chemical fixation protocol for
} bacteria/microorganisms for an EM class that I'm developing for undergrads.
} Since the class hasn't been taught at my university for several years (8), it
} seems that most/all of the microscopy journals have been dropped from the
} libary. So, if you would share your protocol, I would be ever-grateful.
}
} Many thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} 301-687-4697
}
} k.lennon-at-frostburg.edu



==============================Original Headers==============================
6, 27 -- From connellyps-at-nhlbi.nih.gov Tue Jan 20 14:03:53 2009
6, 27 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0KK3qpQ004833
6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2009 14:03:52 -0600
6, 27 -- X-IronPortListener: Outbound_SMTP
6, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116])
6, 27 -- by nihxwayout.hub.nih.gov with ESMTP; 20 Jan 2009 15:03:51 -0500
6, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830);
6, 27 -- Tue, 20 Jan 2009 15:03:46 -0500
6, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.161]) with Microsoft Exchange Server HTTP-DAV ;
6, 27 -- Tue, 20 Jan 2009 20:03:46 +0000
6, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
6, 27 -- Date: Tue, 20 Jan 2009 15:02:57 -0500
6, 27 -- Subject: Re: [Microscopy] Good fix for bacteria/microorganisms?
6, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov}
6, 27 -- To: {kamlennon-at-yahoo.com} ,
6, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
6, 27 -- Message-ID: {C59B9821.2D80%connellyps-at-nhlbi.nih.gov}
6, 27 -- Thread-Topic: [Microscopy] Good fix for bacteria/microorganisms?
6, 27 -- Thread-Index: Acl7OhZYVLjdlOctEd2phwANk2Yv1A==
6, 27 -- In-Reply-To: {200901201955.n0KJtuem000581-at-ns.microscopy.com}
6, 27 -- Mime-version: 1.0
6, 27 -- Content-type: text/plain;
6, 27 -- charset="ISO-8859-1"
6, 27 -- X-OriginalArrivalTime: 20 Jan 2009 20:03:46.0826 (UTC) FILETIME=[340B86A0:01C97B3A]
6, 27 -- Content-Transfer-Encoding: 8bit
6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0KK3qpQ004833
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 21 Jan 2009 07:03:14 -0600
Subject: [Microscopy] Labeling Bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,
I am asking the following on behalf of a colleague:
He wants to label bacteria in order to better see them in microscopy (both fluorescence and SEM). Apparently the morphology is not sufficient to clearly identify them.
In fluorescence, labeling with DAPI worked really well, although the fluorescence bleaches pretty fast.
 
For SEM I thought about staining them with either Osmium, lead or uranyle, which are easy to find in a EM lab. This way there is a good chance to recognize them immediately based on the BSE contrast. If needed an EDX analysis would clear all doubts.
Of course there is a risk that the labeling modifies the interaction with the substrate/material (I thought about labeling them before the binding, otherwise the support may be stained too).
Labeling with antibodies is not an option because it would require to order the antibodies just for this purpose

May I ask your opinion on the question?
Best regards,
Stephane





==============================Original Headers==============================
5, 22 -- From nizets2-at-yahoo.com Wed Jan 21 07:03:14 2009
5, 22 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0LD3Evo030473
5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 07:03:14 -0600
5, 22 -- Received: (qmail 98891 invoked by uid 60001); 21 Jan 2009 13:03:13 -0000
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=yahoo.com;
5, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
5, 22 -- b=dt1hEvJb7DlA8cPy1hwvS6oTOi3Ml9/m50sQC8vcFlpJe9yrcoaTd4wiV76cgFZSytL4m+450g8/MTmwxRJGtJpXq4Yn7QdyD36uJu61sj8n61dKPTBOMs5azsFW178Nr58M68+Q0cUBzH3pmclUHmT2V/XKEHt5wgEUNqQ60i8=;
5, 22 -- X-YMail-OSG: aeKjWUIVM1kI.3GYLfakhaUPAK_nb3G3Ril86bXREmgjDvtNYm3LWrntKcPNbO7sK8.D9TOlgwjwIaZrZR0kil0ezWsBBEF9jLzAw_OgnSMX.pfSefhYqUl9Y6Dz8xnmZjA0QOLZ_xy6T7NlNr2FSsELaof4gnQE0ByMD0wg4gVD7k316lAMwlkSy4AvTw--
5, 22 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Wed, 21 Jan 2009 05:03:13 PST
5, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1
5, 22 -- References: {200901202008.n0KK8a6c013590-at-ns.microscopy.com}
5, 22 -- Date: Wed, 21 Jan 2009 05:03:13 -0800 (PST)
5, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 22 -- Subject: Labeling Bacteria for SEM
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=iso-8859-1
5, 22 -- Message-ID: {778183.98312.qm-at-web110808.mail.gq1.yahoo.com}
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0LD3Evo030473
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 21 Jan 2009 07:36:30 -0600
Subject: [Microscopy] Re: Good fix for bacteria/microorganisms?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kristen,

We use Bacillus subtilus or E. coli in our classes, and fix with
standard Karnovsky's in pH 7.2 buffer, 5 min. steps in EtOH, 30%, 50,
70, 80, 90, 95, 3x100 then CPD or embed. No Prop Ox.
(Or air dry or HMDS for SEM).
Note, for negative stains, we don't fix, except for what fixing the
stain itself does. If you're not planning on doing negative stain in
the class, I would suggest you add that. It's important for clinical
work, and is a good way to get students started on the TEM.
Bozzola and Dysktra both have good negative stain sections, and
Maunsbach & Aufzelius has good comparisons for most TEM methods. If
you don't have that last book, you want it.

Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
4, 25 -- From oshel1pe-at-cmich.edu Wed Jan 21 07:36:29 2009
4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0LDaTAK012838
4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 07:36:29 -0600
4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0LDaRY8022532
4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 08:36:28 -0500
4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Wed, 21 Jan 2009 08:36:11 -0500
4, 25 -- Mime-Version: 1.0
4, 25 -- Message-Id: {f06240809c59cd40ae063-at-[141.209.160.249]}
4, 25 -- In-Reply-To: {200901201952.n0KJqc08031364-at-ns.microscopy.com}
4, 25 -- References: {200901201952.n0KJqc08031364-at-ns.microscopy.com}
4, 25 -- Date: Wed, 21 Jan 2009 08:36:10 -0500
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 25 -- Subject: Re: [Microscopy] Good fix for bacteria/microorganisms?
4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 25 -- X-OriginalArrivalTime: 21 Jan 2009 13:36:11.0969 (UTC) FILETIME=[397BB310:01C97BCD]
4, 25 -- X-Canit-CHI2: 0.00
4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
4, 25 -- X-CanItPRO-Stream: default
4, 25 -- X-Canit-Stats-ID: 7724980 - 95d8e9dab535
4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Wed, 21 Jan 2009 09:16:53 -0600
Subject: [Microscopy] Re: Labeling Bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stéphane (without an ie)

Actually, your question is potentially loaded. What does the colleague
mean by identify microorganisms by microscopy, using fluorescence and
SEM. Simply visualizing by SEM is not a problem. Standard procedures
should work. Striking micrographs of different m.o.s have been published
over the years. I’m not sure what has been done with the projects of
mine over the years - the SEM was all previous to going back to school
and the EM unit was treated in a very cavalier fashion when it came to
recognition. However, at least one resulted in a scanning image of a
Chlamydia trachomatis inclusion body releasing and bursting being placed
on the cover of a book (Sexually transmitted diseases : methods and
protocols, Peeling, RW & Sparling, PF (eds.)). The key in all of this is
not visualizing the m.o., it is identifying it. How do you tell it is
what you see.

Specific antisera should work quite effectively for both
immunofluorescent microscopy, and immunogold labeling with both negative
stain TEM and for SEM. The keys are the quality of the antibody and
accessibility of the target epitope. The epitopes must be on the
external surface if you wish to label them for SEM or NS-TEM and they
will have the greatest chance of success with immunofluorescent
microscopy. I have never done this for SEM, but have for NS-TEM. It has
been over 20 years ago since I did this with N. gonorrhea for negative
stain processing, so I don’t have the notes handy, however, all I did
was give a light fixation (0.1% Glutaraldehyde in PBS), and then react
with gold labeled with an antibody to a major outer protein epitope
using standard protocols, with heavy blocking!!. My memory is that the
target was a porin structure. Also, this was before readily available
commercial gold tagged antibodies, and so I had to make the gold and
label it myself, so it was a very involved process from start to end. In
this case, labeling was not wildly successful, but there was sufficient
specific labeling to clearly identify the external location of the
epitope. At the same time, a monoclonal Ab to C. trachomatis failed
totally using the same protocol. It should be noted that the
investigator never accepted that just because the monoclonal antibody
worked in western blotting of transblotted gels did not mean it would
work in identify targets in a ‘natural’ situation. There was never a
straight answer on whether it worked in IF or EIA of whole m.o.s. The
key here is that you really must stress that success is dependent upon
the ability of the antibodies present to identify the target in its
native state. While that may appear to be a statement of the obvious, it
must be reiterated to all colleagues, collaborators, clients etc that
come in the door, and frequently to ourselves so that we don’t forget it.

Saponin permeablization has worked well for pre-embedding DAB immuno
electron microscopic visualization in my hands. The antibody was good,
and worked well in both IF and TEM. That allowed targeting epitopes of
m.o.s inside cells. At the same time, neither Triton X nor saponin
permeablization allowed immunogold labeling of nucleocapsid proteins of
Lassavirus. Because the antisera to the Z protein was oligopeptide
monospecific (to a 9aa oligopeptide), and I could never get antisera to
the whole protein from that group of collaborators, I do not know if the
failure was due to an ineffective permeablization protocol or
inappropriate antibody. I won’t re-state the obvious from above.

Immunofluorescence is not really the same as pre-embedding immunogold
TEM, even though they may seem to be identical, and my immuno DAB
protocol for labeling was essentially the same as the IF protocol used
in that particular study. This is especially true if your colleague
wants to try targeting internal proteins by IF. It may be a bit more
difficult to access the epitopes. Penetrating the dense, hydrophobic
outer structures of mycobacteria, or of mycoplasm would be a challenge.
Having said that, I have used standard TEM preparative procedures to fix
and embed both for sectioning, and have done immunogold labelling for
internal proteins successfully with other m.o.s (although we did not
realize the protein was internal when they collaborator gave me the
material, and it took a little work to define what was happening when we
saw the results. They were crystal clear once it was established that
the epitope was on the internal face of the membrane.) Because many
others have also had good success with both pre and post embedding
immunogold labeling of m.o.s for internal epitopes, I would suggest that
standard IF fixation and labeling would work. Again, to restate the
obvious, provided the antibodies will recognize the target.

I have to restate the obvious to many people who walk in the door
wanting to discuss either EM or gastroenteric virology. Sorry about
repeating it, but it seems to be an occupational hazard here.

I can dig through the archives and find protocols if you want. Most of
those in the lit should work, that’s where mine came from.

Paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 21 09:16:52 2009
11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0LFGq16030890
11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 09:16:52 -0600
11, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
11, 20 -- (authenticated bits=0)
11, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n0LFGoPM021281;
11, 20 -- Wed, 21 Jan 2009 09:16:51 -0600 (CST)
11, 20 -- Message-ID: {49773C61.3030306-at-umanitoba.ca}
11, 20 -- Date: Wed, 21 Jan 2009 09:16:49 -0600
11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
11, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
11, 20 -- MIME-Version: 1.0
11, 20 -- To: nizets2-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com}
11, 20 -- Subject: Re: [Microscopy] Labeling Bacteria for SEM
11, 20 -- References: {200901211305.n0LD5Q58000922-at-ns.microscopy.com}
11, 20 -- In-Reply-To: {200901211305.n0LD5Q58000922-at-ns.microscopy.com}
11, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 20 -- Content-Transfer-Encoding: 8bit
11, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: pavig-at-asu.edu
Date: Wed, 21 Jan 2009 15:54:43 -0600
Subject: [Microscopy] viaWWW: Quantitating virus particles in cells following TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pavig-at-asu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pavig-at-asu.edu
Name: Pavithra Venkatagopalan

Organization: Arizona State University

Title-Subject: [Filtered] Quantitating virus particles in cells following TEM

Question: Hello,
I have to compare the number of intracellular virus particles in
infected cells using TEM. I would like to know if there is precedent
for such a calculation and if so, what is an unbiased method to
obtain this data.

Thanks

Login Host: 209.147.154.184
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Jan 21 15:54:43 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0LLsfgu029213
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 15:54:42 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c59d4a0ec637-at-[206.69.208.22]}
7, 11 -- Date: Wed, 21 Jan 2009 15:54:40 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: pavig-at-asu.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Quantitating virus particles in cells following TEM
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hyi-at-emory.edu
Date: Wed, 21 Jan 2009 23:30:05 -0600
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:

We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper=
ience comment on how long (or how many blocks) I should expect a Histo diam=
ond knife to last? I have no problem producing high quality semi-thin sect=
ions with glass knives, but am hoping a diamond knife would save us some ti=
me. Thank you in advance.

Hong
Emory EM


This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).


==============================Original Headers==============================
7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009
7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94])
7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498
7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600
7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756
7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500
7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by
7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server
7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500
7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009
7, 32 -- 00:29:58 -0500
7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500
7, 32 -- Subject: Histo diamond knife
7, 32 -- Thread-Topic: Histo diamond knife
7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ==
7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu}
7, 32 -- Accept-Language: en-US
7, 32 -- Content-Language: en
7, 32 -- X-MS-Has-Attach:
7, 32 -- X-MS-TNEF-Correlator:
7, 32 -- acceptlanguage: en-US
7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
7, 32 -- MIME-Version: 1.0
7, 32 -- X-emory.edu-MailScanner: Found to be clean
7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
7, 32 -- X-Spam-Status: No
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 22 Jan 2009 02:41:25 -0600
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Hong!

We have a histo knife, however we don't cut thicker than 500nm and not on a routine basis.
I have been said that like an ultraknife, its lifetime mainly depends on what you cut. Cut butter and it will survive you.
Cut nanoparticles and quantum dots and it will probably not survive your grant. Cutting soft tissue in resin does probably not significantly affect it.
Personally I couldn't imagine regularly semi-thin sectionning without histoknife, it is so comfortable. Maybe I am a luxus freak :-) 

Regards,
Stephane



----- Original Message ----
X-from: "hyi-at-emory.edu" {hyi-at-emory.edu}
To: nizets2-at-yahoo.com
Sent: Thursday, January 22, 2009 6:34:37 AM

Dear All:

    We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
e for semi-thin (0.5-0.7=B5m) sectioning.  Can someone out there with exper=
ience comment on how long (or how many blocks) I should expect a Histo diam=
ond knife to last?  I have no problem producing high quality semi-thin sect=
ions with glass knives, but am hoping a diamond knife would save us some ti=
me.  Thank you in advance.

Hong
Emory EM


This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information.  If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).


==============================Original Headers==============================
7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009
7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94])
7, 32 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498
7, 32 --     for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600
7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
7, 32 --     by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756
7, 32 --     for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500
7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by
7, 32 --  EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server
7, 32 --  (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500
7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
7, 32 --  EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009
7, 32 --  00:29:58 -0500
7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500
7, 32 -- Subject: Histo diamond knife
7, 32 -- Thread-Topic: Histo diamond knife
7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ==
7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu}
7, 32 -- Accept-Language: en-US
7, 32 -- Content-Language: en
7, 32 -- X-MS-Has-Attach:
7, 32 -- X-MS-TNEF-Correlator:
7, 32 -- acceptlanguage: en-US
7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
7, 32 -- MIME-Version: 1.0
7, 32 -- X-emory.edu-MailScanner: Found to be clean
7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
7, 32 -- X-Spam-Status: No
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498
==============================End of - Headers==============================






==============================Original Headers==============================
22, 22 -- From nizets2-at-yahoo.com Thu Jan 22 02:41:24 2009
22, 22 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0M8fO8J012980
22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 02:41:24 -0600
22, 22 -- Received: (qmail 62862 invoked by uid 60001); 22 Jan 2009 08:41:23 -0000
22, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
22, 22 -- s=s1024; d=yahoo.com;
22, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
22, 22 -- b=arER5TCHEvR9cLCGy/Stts2255pSPF+6wxsQAw5DCTUcTxQvNs4cibc6EFv+GwnvxHoCBYwRbkyn7DxspB5qSxEjMf5rV+boK90JSFsUlFw9b3w/LGbsrLdyy0goNk4HGLfs9mH0CixEVfqBLjPlFDmafbqbAdhEYQ8pH5avEvQ=;
22, 22 -- X-YMail-OSG: 98.knsQVM1n7xyKC0afwsbtGUfk4_PDhvvFu_Xs.c_vWUIPV.VfRtFQjDBQRBez96A4u5S.icydUpBr.GBpx.UeMFtxK1j6KAIaPJSSQcvAc17ghYIcQp3Z5bRsOW03oeiZYAKQ8arb1moDp5RosesU1lpvifrHF_FbFIsBu7f.TTn0Z4O_uC6VdNHvNjGkl27vz1kMLBxUV4c9jIzVtAgja4CoF1Qh7
22, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 22 Jan 2009 00:41:23 PST
22, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1
22, 22 -- References: {200901220534.n0M5Ybmx030096-at-ns.microscopy.com}
22, 22 -- Date: Thu, 22 Jan 2009 00:41:23 -0800 (PST)
22, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
22, 22 -- Subject: Re: [Microscopy] Histo diamond knife
22, 22 -- To: microscopy-at-microscopy.com
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain; charset=iso-8859-1
22, 22 -- Message-ID: {897590.62667.qm-at-web110805.mail.gq1.yahoo.com}
22, 22 -- Content-Transfer-Encoding: 8bit
22, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M8fO8J012980
==============================End of - Headers==============================




From: lamiller-at-illinois.edu
Date: Thu, 22 Jan 2009 07:54:05 -0600
Subject: [Microscopy] Re: Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I love our histo knife.

I use an old diamond knife to face the block, then switch out to the
histo knife. Sectioning is done at 0.33 µm.

Life span varies with usage, type of sample ( ie bone or cell culture
phosphate crystals, etc are harder on the knife)

I once had a histo knife that I had cut about , say 500-600 blocks /
year, and I cut big blocks often, it lasted for 7 years!

However , get glass, silicone, bone etc, and you could ruin a knife in
a day.

The time saved is really huge, the quality is very good, especially if
you have to section some to get to "just the right depth".


Well worth the money, and yes when I switched I did have a little bit
of prideful.... I can do glass well and it's an art... thing, that
goes to the wayside quick after the pleasure of working with a diamond
histo knife.

So save old knives, use them to rough cut the already trimmed block,
and you will get even longer life out of your knife.



Lou Ann


{ { { { { { { { {} } } } } } } } } } } } } }
Lou Ann Miller, Service Supervisor
Center for Microscopic Imaging
College of Veterinary Medicine
University of Illinois MC=002

Room 1204 VMBSBld
2001 S Lincoln Ave
Urbana, IL 61821

217-244-1567




On Jan 21, 2009, at 11:37 PM, hyi-at-emory.edu wrote:

}
}
} Dear All:
}
} We have been contemplating about purchasing a 8.0 mm Histo
} diamond knif=
} e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there
} with exper=
} ience comment on how long (or how many blocks) I should expect a
} Histo diam=
} ond knife to last? I have no problem producing high quality semi-
} thin sect=
} ions with glass knives, but am hoping a diamond knife would save us
} some ti=
} me. Thank you in advance.
}
} Hong
} Emory EM
}


==============================Original Headers==============================
22, 19 -- From lamiller-at-illinois.edu Thu Jan 22 07:54:05 2009
22, 19 -- Received: from expredir6.cites.uiuc.edu (expredir6.cites.uiuc.edu [128.174.5.97])
22, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MDs4hX012649
22, 19 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 07:54:05 -0600
22, 19 -- Received: from beowulf.cvm.uiuc.edu (beowulf.cvm.uiuc.edu [130.126.16.163])
22, 19 -- by expredir6.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id n0MDs4tP005337;
22, 19 -- Thu, 22 Jan 2009 07:54:04 -0600 (CST)
22, 19 -- Message-Id: {976ED5E6-1783-4E20-9F13-2EC599CE1B3B-at-illinois.edu}
22, 19 -- From: Lou Ann Miller {lamiller-at-illinois.edu}
22, 19 -- To: microscopy-at-microscopy.com, hyi-at-emory.edu
22, 19 -- In-Reply-To: {200901220537.n0M5bKFP002839-at-ns.microscopy.com}
22, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
22, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
22, 19 -- Subject: Re: [Microscopy] Histo diamond knife
22, 19 -- Date: Thu, 22 Jan 2009 07:54:04 -0600
22, 19 -- References: {200901220537.n0M5bKFP002839-at-ns.microscopy.com}
22, 19 -- X-Mailer: Apple Mail (2.930.3)
22, 19 -- Content-Transfer-Encoding: 8bit
22, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MDs4hX012649
==============================End of - Headers==============================




From: allan.mitchell-at-stonebow.otago.ac.nz
Date: Thu, 22 Jan 2009 08:11:30 -0600
Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both allan.mitchell-at-stonebow.otago.ac.nz as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: allan.mitchell-at-stonebow.otago.ac.nz
Name: Allan Mitchell

Organization: University of otago

Title-Subject: [Filtered] TEM of TiO2 nanotubes

Question: Hi all

I have been working with a researcher here looking at some TiO2
Nanotubes in the TEM. The researcher wants to see if his nanotubes
are hollow or not. We are looking for a core of around 5 nm.

Being a biology lab we do not have any experience with such samples.
I have tried dusting the fragments (provided in powder form from
scrappings off a Ti plate) onto carbon/formvar grids and I have
tried drying them onto a grid from a suspension in ethanol. The
solvent method proved more successful at getting particles onto the
film than the dusting method however in both cases they tend to be
clumped.

The problem I have is the clumps must be heating up in the beam. I
as soon as I start to increase the magnification to explore the edges
the sample disappears and I am left with a hole in the film.

I am pushing a 100kV instrument so I suspect this may have something
to with it also.

A search of the literature indicates that a lot of people are
investigating TiO2 nanotubes in the TEM but nothing I have found so
far talks about how the nanotubes are got onto a grid in a usable
form to image. lots of info about making nanotubes.

Any thoughts or suggestions would be appreciated.

Regards

Allan


Allan Mitchell
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
Department: http://anatomy.otago.ac.nz/

Login Host: 139.80.40.92
---------------------------------------------------------------------------

==============================Original Headers==============================
18, 11 -- From zaluzec-at-microscopy.com Thu Jan 22 08:11:29 2009
18, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
18, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MEBTKc026853
18, 11 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 08:11:29 -0600
18, 11 -- Mime-Version: 1.0
18, 11 -- Message-Id: {p06240801c59e2edb6749-at-[206.69.208.22]}
18, 11 -- Date: Thu, 22 Jan 2009 08:11:28 -0600
18, 11 -- To: microscopy-at-microscopy.com
18, 11 -- From: allan.mitchell-at-stonebow.otago.ac.nz (by way of MicroscopyListserver)
18, 11 -- Subject: viaWWW: TEM of TiO2 nanotubes
18, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Thu, 22 Jan 2009 09:14:12 -0600
Subject: [Microscopy] Re: Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Hong,
Don't contemplate - spend the money, get the knife - make yourself
happy. You will not regret it nor will you go back to using glass.
Consider it a necessary luxury item - you will feel so spoiled every
time you use it. Work productivity will increase tenfold. Everyone
here uses them for 1um thick sections (plant material).

It's the best investment you will make in 2009;-)
Beth


On Jan 22, 2009, at 12:30 AM, hyi-at-emory.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All:
}
} We have been contemplating about purchasing a 8.0 mm Histo
} diamond knif=
} e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there
} with exper=
} ience comment on how long (or how many blocks) I should expect a
} Histo diam=
} ond knife to last? I have no problem producing high quality semi-
} thin sect=
} ions with glass knives, but am hoping a diamond knife would save us
} some ti=
} me. Thank you in advance.
}
} Hong
} Emory EM
}
}
} This e-mail message (including any attachments) is for the sole use of
} the intended recipient(s) and may contain confidential and privileged
} information. If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination,
} distribution
} or copying of this message (including any attachments) is strictly
} prohibited.
}
} If you have received this message in error, please contact
} the sender by reply e-mail message and destroy all copies of the
} original message (including attachments).
}
}
} ==============================Original
} Headers==============================
} 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009
} 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu
} [170.140.52.94])
} 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n0M5U5Rv023498
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05
} -0600
} 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net
} (emoryfloatdmz.cc.emory.edu [170.140.52.254])
} 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id
} n0M5U0qO021756
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00
} -0500
} 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net
} (170.140.30.56) by
} 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with
} Microsoft SMTP Server
} 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500
} 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net
} ([10.128.11.12]) by
} 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi;
} Thu, 22 Jan 2009
} 7, 32 -- 00:29:58 -0500
} 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500
} 7, 32 -- Subject: Histo diamond knife
} 7, 32 -- Thread-Topic: Histo diamond knife
} 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ==
} 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu}
} 7, 32 -- Accept-Language: en-US
} 7, 32 -- Content-Language: en
} 7, 32 -- X-MS-Has-Attach:
} 7, 32 -- X-MS-TNEF-Correlator:
} 7, 32 -- acceptlanguage: en-US
} 7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
} 7, 32 -- MIME-Version: 1.0
} 7, 32 -- X-emory.edu-MailScanner: Found to be clean
} 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
} 7, 32 -- X-Spam-Status: No
} 7, 32 -- Content-Transfer-Encoding: 8bit
} 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n0M5U5Rv023498
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
7, 20 -- From beth-at-plantbio.uga.edu Thu Jan 22 09:14:12 2009
7, 20 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MFECik010739
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 09:14:12 -0600
7, 20 -- Received: from [128.192.26.46] ([128.192.26.46])
7, 20 -- (authenticated user beth-at-plantbio.uga.edu)
7, 20 -- by dogwood.plantbio.uga.edu
7, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits));
7, 20 -- Thu, 22 Jan 2009 10:14:06 -0500
7, 20 -- Message-Id: {5A3544FC-664B-4CD8-98A4-7BBE186A69AA-at-plantbio.uga.edu}
7, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
7, 20 -- To: Yi Hong {hyi-at-emory.edu} , microscopy microscopy {microscopy-at-microscopy.com}
7, 20 -- In-Reply-To: {200901220530.n0M5UwAq024392-at-ns.microscopy.com}
7, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2)
7, 20 -- Subject: Re: [Microscopy] Histo diamond knife
7, 20 -- Date: Thu, 22 Jan 2009 10:14:09 -0500
7, 20 -- References: {200901220530.n0M5UwAq024392-at-ns.microscopy.com}
7, 20 -- X-Mailer: Apple Mail (2.929.2)
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Thu, 22 Jan 2009 12:28:12 -0600
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Hong,
I agree with Beth. Do yourself a favor, get the knife! It will save you alot
of time.
We use diamond knives for all of our thicks. The only time we have to make
glass knives is when we cut something that may have bone or hard material
and then we also use glass to cut thins. If you take care of the knife as
you probably do the ultra-knives, you will get alot of sections off of it.
Pat Kysar
University of California, Davis
Medical School, Pathology
EM Lab

----- Original Message -----
X-from: {beth-at-plantbio.uga.edu}
To: {pekysar-at-ucdavis.edu}
Sent: Thursday, January 22, 2009 7:21 AM

We purchased two for serial sectioning of fish embryos at 2 microns and
are very pleased with them. For serial sections re-aligning for each
fresh glass knife is out
of the question. When one knife develops nicks (after many thousands of
sections) and is being resharpened we use the second knife.

Geoff

hyi-at-emory.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All:
}
} We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
} e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper=
} ience comment on how long (or how many blocks) I should expect a Histo diam=
} ond knife to last? I have no problem producing high quality semi-thin sect=
} ions with glass knives, but am hoping a diamond knife would save us some ti=
} me. Thank you in advance.
}
} Hong
} Emory EM
}
}
} This e-mail message (including any attachments) is for the sole use of
} the intended recipient(s) and may contain confidential and privileged
} information. If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination, distribution
} or copying of this message (including any attachments) is strictly
} prohibited.
}
} If you have received this message in error, please contact
} the sender by reply e-mail message and destroy all copies of the
} original message (including attachments).
}
}
} ==============================Original Headers==============================
} 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009
} 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94])
} 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600
} 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
} 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500
} 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by
} 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server
} 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500
} 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
} 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009
} 7, 32 -- 00:29:58 -0500
} 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500
} 7, 32 -- Subject: Histo diamond knife
} 7, 32 -- Thread-Topic: Histo diamond knife
} 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ==
} 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu}
} 7, 32 -- Accept-Language: en-US
} 7, 32 -- Content-Language: en
} 7, 32 -- X-MS-Has-Attach:
} 7, 32 -- X-MS-TNEF-Correlator:
} 7, 32 -- acceptlanguage: en-US
} 7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
} 7, 32 -- MIME-Version: 1.0
} 7, 32 -- X-emory.edu-MailScanner: Found to be clean
} 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
} 7, 32 -- X-Spam-Status: No
} 7, 32 -- Content-Transfer-Encoding: 8bit
} 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498
} ==============================End of - Headers==============================
}
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
7, 28 -- From mcauliff-at-umdnj.edu Thu Jan 22 09:59:20 2009
7, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124])
7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0MFxJsL030907
7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 09:59:19 -0600
7, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
7, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 48A4EA7C2C
7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 10:59:16 -0500 (EST)
7, 28 -- Received: from umdnj.edu (unknown [10.32.15.102])
7, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 1A32BA7C2F
7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 10:32:21 -0500 (EST)
7, 28 -- Received: from ([10.32.15.171])
7, 28 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.4981654;
7, 28 -- Thu, 22 Jan 2009 10:30:34 -0500
7, 28 -- MIME-version: 1.0
7, 28 -- Content-transfer-encoding: 7BIT
7, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
7, 28 -- Received: from [127.0.0.1] ([10.32.15.102])
7, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
7, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KDV00ICJPQX82C0-at-umduwc02.umdnj.edu} for
7, 28 -- microscopy-at-microscopy.com; Thu, 22 Jan 2009 10:30:34 -0500 (EST)
7, 28 -- Message-id: {49789165.3080400-at-umdnj.edu}
7, 28 -- Date: Thu, 22 Jan 2009 10:31:49 -0500
7, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
7, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
7, 28 -- To: hyi-at-emory.edu, microscopy-at-microscopy.com
7, 28 -- Subject: Re: [Microscopy] Histo diamond knife
7, 28 -- References: {200901220531.n0M5VPPb024975-at-ns.microscopy.com}
7, 28 -- In-reply-to: {200901220531.n0M5VPPb024975-at-ns.microscopy.com}
==============================End of - Headers==============================

From stimulus-at-vodafone.net Thu Jan 22 11:41:54 2009
Return-Path: {stimulus-at-vodafone.net}
Received: from inpac.com.cn ([220.194.209.44])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MHfqjI031697
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 22 Jan 2009 11:41:53 -0600
Received: from User [88.40.105.35] by inpac.com.cn with ESMTP
(SMTPD-10.01) id AF5F03B8; Fri, 23 Jan 2009 01:39:43 +0800

I recommend the Histo diamond knife. At my previous job, we didn't have any complaints sectioning tissues and cell pellets at a half micron thick. By removing the chance of glass dust getting on the ultrathin diamond knife, I believe it lasted longer without nicks as well. When we used glass knives, we would face the block between glass and ultrathin diamind knife work (to remove any rare glass bits) using an old sapphire knife. I was glad to skip that step after switching to the diamond histo knife.

For one project, I sectioned a 4-5mm wide tissue section with the histo knife. I wouldn't have wanted to try that with glass!

~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA



-----Original Message-----
X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu]
Sent: Thursday, January 22, 2009 12:38 AM
To: Sobocinski, Gregg

Dear All:

We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper=
ience comment on how long (or how many blocks) I should expect a Histo diam=
ond knife to last? I have no problem producing high quality semi-thin sect=
ions with glass knives, but am hoping a diamond knife would save us some ti=
me. Thank you in advance.

Hong
Emory EM


==============================Original Headers==============================
15, 27 -- From greggps-at-umich.edu Thu Jan 22 12:28:12 2009
15, 27 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201])
15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MISCcv002582
15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:28:12 -0600
15, 27 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by
15, 27 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Thu, 22 Jan
15, 27 -- 2009 13:28:09 -0500
15, 27 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
15, 27 -- To: "hyi-at-emory.edu" {hyi-at-emory.edu} ,
15, 27 -- "microscopy-at-microscopy.com"
15, 27 -- {microscopy-at-microscopy.com}
15, 27 -- Date: Thu, 22 Jan 2009 13:28:08 -0500
15, 27 -- Subject: RE: [Microscopy] Histo diamond knife
15, 27 -- Thread-Topic: [Microscopy] Histo diamond knife
15, 27 -- Thread-Index: Acl8U4r949TpifdHQNKnQ2lv7VkOUAAadtUw
15, 27 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA3934016D4652-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
15, 27 -- References: {200901220537.n0M5bcNu003401-at-ns.microscopy.com}
15, 27 -- In-Reply-To: {200901220537.n0M5bcNu003401-at-ns.microscopy.com}
15, 27 -- Accept-Language: en-US
15, 27 -- Content-Language: en-US
15, 27 -- X-MS-Has-Attach:
15, 27 -- X-MS-TNEF-Correlator:
15, 27 -- acceptlanguage: en-US
15, 27 -- Content-Type: text/plain; charset="us-ascii"
15, 27 -- MIME-Version: 1.0
15, 27 -- Content-Transfer-Encoding: 8bit
15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MISCcv002582
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thursday, January 22, 2009, 6:36 PM
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I also recommend a histo diamond. I even used one to cut thin sections of intact squid tentacle tip which were several mm wide.

Does any one out there have any experience with the Pella histo diamond? I have a user who is interested in it because it comes in a 10mm edge. I've only used an 8mm Diatome.

Any comments or suggestions would be appreciated.

You can reply offline if you prefer.

Paula :-)

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF)

Core Microscope Facility, room B141

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397

--- On Thu, 1/22/09, greggps-at-umich.edu {greggps-at-umich.edu} wrote:
X-from: greggps-at-umich.edu {greggps-at-umich.edu}

I recommend the Histo diamond knife. At my previous job, we didn't have any
complaints sectioning tissues and cell pellets at a half micron thick. By
removing the chance of glass dust getting on the ultrathin diamond knife, I
believe it lasted longer without nicks as well. When we used glass knives, we
would face the block between glass and ultrathin diamind knife work (to remove
any rare glass bits) using an old sapphire knife. I was glad to skip that step
after switching to the diamond histo knife.

For one project, I sectioned a 4-5mm wide tissue section with the histo knife.
I wouldn't have wanted to try that with glass!

~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA



-----Original Message-----
X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu]
Sent: Thursday, January 22, 2009 12:38 AM
To: Sobocinski, Gregg

Dear All:

We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper=
ience comment on how long (or how many blocks) I should expect a Histo diam=
ond knife to last? I have no problem producing high quality semi-thin sect=
ions with glass knives, but am hoping a diamond knife would save us some ti=
me. Thank you in advance.

Hong
Emory EM


==============================Original Headers==============================
15, 27 -- From greggps-at-umich.edu Thu Jan 22 12:28:12 2009
15, 27 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu
(itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201])
15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0MISCcv002582
15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:28:12
-0600
15, 27 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu
([141.211.3.233]) by
15, 27 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi;
Thu, 22 Jan
15, 27 -- 2009 13:28:09 -0500
15, 27 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
15, 27 -- To: "hyi-at-emory.edu" {hyi-at-emory.edu} ,
15, 27 -- "microscopy-at-microscopy.com"
15, 27 -- {microscopy-at-microscopy.com}
15, 27 -- Date: Thu, 22 Jan 2009 13:28:08 -0500
15, 27 -- Subject: RE: [Microscopy] Histo diamond knife
15, 27 -- Thread-Topic: [Microscopy] Histo diamond knife
15, 27 -- Thread-Index: Acl8U4r949TpifdHQNKnQ2lv7VkOUAAadtUw
15, 27 -- Message-ID:
{9F8ADD9ABC7F264E82EDDE4C10DA3934016D4652-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
15, 27 -- References: {200901220537.n0M5bcNu003401-at-ns.microscopy.com}
15, 27 -- In-Reply-To: {200901220537.n0M5bcNu003401-at-ns.microscopy.com}
15, 27 -- Accept-Language: en-US
15, 27 -- Content-Language: en-US
15, 27 -- X-MS-Has-Attach:
15, 27 -- X-MS-TNEF-Correlator:
15, 27 -- acceptlanguage: en-US
15, 27 -- Content-Type: text/plain; charset="us-ascii"
15, 27 -- MIME-Version: 1.0
15, 27 -- Content-Transfer-Encoding: 8bit
15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n0MISCcv002582
==============================End of - Headers==============================






==============================Original Headers==============================
35, 25 -- From vapatpxs-at-yahoo.com Thu Jan 22 12:42:13 2009
35, 25 -- Received: from n26.bullet.mail.mud.yahoo.com (n26.bullet.mail.mud.yahoo.com [68.142.206.221])
35, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0MIgDRB016839
35, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:42:13 -0600
35, 25 -- Received: from [68.142.200.226] by n26.bullet.mail.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000
35, 25 -- Received: from [68.142.201.244] by t7.bullet.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000
35, 25 -- Received: from [127.0.0.1] by omp405.mail.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000
35, 25 -- X-Yahoo-Newman-Property: ymail-3
35, 25 -- X-Yahoo-Newman-Id: 962966.68788.bm-at-omp405.mail.mud.yahoo.com
35, 25 -- Received: (qmail 40504 invoked by uid 60001); 22 Jan 2009 18:42:12 -0000
35, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
35, 25 -- s=s1024; d=yahoo.com;
35, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Message-ID;
35, 25 -- b=Nyn5x5r38szoBwO80QCw0QfYvcicKMbcv+lXHHwA8LPsA514QXgTbOJcP/TkbcVe7QMrH+E87nUfVVCSGzzjIMTL9YA0ZPFzUVQD0galZi2R0d7XP1olhvmDZp7XAQlxSy+R6kRm807TfGfWHEs93XEZBkP6uPupndSPG1YUJfw=;
35, 25 -- X-YMail-OSG: zFz2rw8VM1nNWh.LXWRMhojFaMDWDSoWIXLfqWd5.V5WjNPCefuyMwTpxTeWqU3KrU3wFcjeeVqErsliP2gOjlIwRQg.JuByP7hBoCDmg7QCk9MTubUpOaZ28dXbMXL8xVWytNcmBfx1.VkKz7Xrj5Kj5WEJKfZR1BHYv0ueKP7ye.A3hVmCV7pFRHnkdgk-
35, 25 -- Received: from [132.239.85.200] by web46115.mail.sp1.yahoo.com via HTTP; Thu, 22 Jan 2009 10:42:12 PST
35, 25 -- X-Mailer: YahooMailWebService/0.7.260.1
35, 25 -- Date: Thu, 22 Jan 2009 10:42:12 -0800 (PST)
35, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
35, 25 -- Subject: Re: [Microscopy] RE: Histo diamond knife
35, 25 -- To: MSA BB {Microscopy-at-microscopy.com}
35, 25 -- In-Reply-To: {200901221836.n0MIaBBj014721-at-ns.microscopy.com}
35, 25 -- MIME-Version: 1.0
35, 25 -- Content-Type: text/plain; charset=us-ascii
35, 25 -- Message-ID: {282390.38574.qm-at-web46115.mail.sp1.yahoo.com}
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 22 Jan 2009 13:26:00 -0600
Subject: [Microscopy] LaB6 problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
since some weeks I am trying to bring a new Kimball LaB6 cathode type
423E90 up to performance.
I am using the cathode in a Philips 525 SEM.
My experience with the cathodes before had been that the heating current
had been with the old cathodes at 11 to 13 max. The new cathode needs to
have 14 to 16 max.
I am using the LaB6 wehnelt cap and set the height (with a 0.5mm wehnelt
aperture) at ca. 0,25mm down from the surface of the aperture disc.

Please have a look at the images:
www.elektronenmikroskopie.info/lab6

With heating current at position 11 I am still getting double contours
in the image (LaB6_3.jpg), which is getting better when I am using a
smaller spotsize (LaB6_2.jpg).
With heating current at position 14 I still have some "feeling" of
double contours in the image (see LaB6_4.jpg), which disapears at
smaller spotsize (LaB6_5.jpg).
Best image so far is LaB6_8.jpg at 10nm spotsize and heating current
position of 16 (which seems for me to be too far up the scale).

...I put the cathode in my EM420 TEM and looked at the cathode image (I
am not able to do this in the SEM...).
At high beam current and at heating current position ca. 12 (which is 2
steps more than on older cathodes) the flat tip of the cathode showed up
nicely, resembling a "cross" and comes to an even illumination at
position 14.

My question is:
Is anybody out there giving me some tips if heating values might be
correct? Is there a problem with the wehnelt distance?
I never experienced such a behavior before...
Is there a chance of cathode charging or an inappropriate value of the
wehnelt voltage? I changed wehnelt values, with no better imaging.

Best regards,
Stefan



--
---------------------------------------------------------------------------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49 - 931 - 7848700 Phone
++49 - 931 - 7848701 Fax
++49 - 175 - 717 70 51 Cell-Phone

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://mail.map24.com/stefan.diller
---------------------------------------------------------------------------------------------------------------------

==============================Original Headers==============================
10, 20 -- From stefan.diller-at-t-online.de Thu Jan 22 13:26:00 2009
10, 20 -- Received: from mailout06.t-online.de (mailout06.t-online.de [194.25.134.19])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MJPxMe032561
10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 13:26:00 -0600
10, 20 -- Received: from fwd07.aul.t-online.de
10, 20 -- by mailout06.sul.t-online.de with smtp
10, 20 -- id 1LQ5Be-0006vV-00; Thu, 22 Jan 2009 20:25:58 +0100
10, 20 -- Received: from [127.0.0.1] (ThoUxBZXwhDImFeCWP50SQqpGtEvj7b8zqfaiygx1FUsZl3f3L2rLA+0TI5HNsDgs4-at-[91.10.209.178]) by fwd07.aul.t-online.de
10, 20 -- with esmtp id 1LQ5Ba-1GbVmi0; Thu, 22 Jan 2009 20:25:54 +0100
10, 20 -- Message-ID: {4978C84C.8000507-at-t-online.de}
10, 20 -- Date: Thu, 22 Jan 2009 20:26:04 +0100
10, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
10, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
10, 20 -- MIME-Version: 1.0
10, 20 -- To: microscopy-at-microscopy.com
10, 20 -- Subject: LaB6 problems
10, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
10, 20 -- Content-Transfer-Encoding: 7bit
10, 20 -- X-ID: ThoUxBZXwhDImFeCWP50SQqpGtEvj7b8zqfaiygx1FUsZl3f3L2rLA+0TI5HNsDgs4
10, 20 -- X-TOI-MSGID: 3052e9f8-e686-4f18-a606-ba0fac4b8829
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 22 Jan 2009 13:54:13 -0600
Subject: [Microscopy] Re: LaB6 problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 22, 2009, at 11:26 AM, stefan.diller-at-t-online.de wrote:

} since some weeks I am trying to bring a new Kimball LaB6 cathode type
} 423E90 up to performance.
} I am using the cathode in a Philips 525 SEM.
} My experience with the cathodes before had been that the heating
} current
} had been with the old cathodes at 11 to 13 max. The new cathode
} needs to
} have 14 to 16 max.
} I am using the LaB6 wehnelt cap and set the height (with a 0.5mm
} wehnelt
} aperture) at ca. 0,25mm down from the surface of the aperture disc.
}
} Please have a look at the images:
} www.elektronenmikroskopie.info/lab6
}
} With heating current at position 11 I am still getting double contours
} in the image (LaB6_3.jpg), which is getting better when I am using a
} smaller spotsize (LaB6_2.jpg).
} With heating current at position 14 I still have some "feeling" of
} double contours in the image (see LaB6_4.jpg), which disapears at
} smaller spotsize (LaB6_5.jpg).
} Best image so far is LaB6_8.jpg at 10nm spotsize and heating current
} position of 16 (which seems for me to be too far up the scale).
}
} ...I put the cathode in my EM420 TEM and looked at the cathode image
} (I
} am not able to do this in the SEM...).
} At high beam current and at heating current position ca. 12 (which
} is 2
} steps more than on older cathodes) the flat tip of the cathode
} showed up
} nicely, resembling a "cross" and comes to an even illumination at
} position 14.
}
} My question is:
} Is anybody out there giving me some tips if heating values might be
} correct? Is there a problem with the wehnelt distance?
} I never experienced such a behavior before...
} Is there a chance of cathode charging or an inappropriate value of the
} wehnelt voltage? I changed wehnelt values, with no better imaging.


Dear Stefan,
If you are comparing the Kimball LaB6 to another brand, then it is
not too surprising that the heating currents are different. Kimball
mounts the LaB6 crystal in a cup, which is heated and transfers the
heat to the filament; whereas, some other brands allow the current to
go through the LaB6 directly. There may well be differences in heat
transfer that require a higher current for the Kimball. In any event,
it is best to operate with the filament saturated (assuming that you
are not interested in operating in tip mode, where only the flat of
the filament emits electrons). If the higher current required makes
the life of the tip too short, then you may want to use a different
brand of tip; however, our experience with Kimball LaB6 tips has been
quite good. Our experience may not be too good a guide, since we have
only TEMs, but I think the requirements for good performance are
pretty much the same--high brightness and good coherence.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Thu Jan 22 13:54:12 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MJsBV2014495
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 13:54:11 -0600
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 2E911329E5E
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:11 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id CEC88328F6E
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:09 -0800 (PST)
6, 22 -- Message-Id: {6705092A-D812-4998-B81C-0596D24E8071-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] LaB6 problems
6, 22 -- Date: Thu, 22 Jan 2009 11:54:09 -0800
6, 22 -- References: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Thu, 22 Jan 2009 14:53:53 -0600
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Hong,
We have four large histoknives, usually one for trimming, two for everyday
use, and one in perfect shape for when one of the others has to go away for
resharpening. They are used to cut sections up to 2 microns thick, of quite
large blockfaces - up to 3 mm across. They get resharpened at least once a
year. Like Stephane, I'll never go back to routine glass knife use, the
diamond knives save so much time. We only go back to glass for training and
if the tissue might damage the diamond (chunks of rock in soil around roots,
for example...).

cheers,
Roseamry


On 22/01/09 7:47 PM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Hong!
}
} We have a histo knife, however we don't cut thicker than 500nm and not on a
} routine basis.
} I have been said that like an ultraknife, its lifetime mainly depends on what
} you cut. Cut butter and it will survive you.
} Cut nanoparticles and quantum dots and it will probably not survive your
} grant. Cutting soft tissue in resin does probably not significantly affect it.
} Personally I couldn't imagine regularly semi-thin sectionning without
} histoknife, it is so comfortable. Maybe I am a luxus freak :-) 
}
} Regards,
} Stephane
}
}
}
} ----- Original Message ----
} X-from: "hyi-at-emory.edu" {hyi-at-emory.edu}
} To: nizets2-at-yahoo.com
} Sent: Thursday, January 22, 2009 6:34:37 AM
} Subject: [Microscopy] Histo diamond knife
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All:
}
}     We have been contemplating about purchasing a 8.0 mm Histo diamond knif=
} e for semi-thin (0.5-0.7=B5m) sectioning.  Can someone out there with exper=
} ience comment on how long (or how many blocks) I should expect a Histo diam=
} ond knife to last?  I have no problem producing high quality semi-thin sect=
} ions with glass knives, but am hoping a diamond knife would save us some ti=
} me.  Thank you in advance.
}
} Hong
} Emory EM
}
}
} This e-mail message (including any attachments) is for the sole use of
} the intended recipient(s) and may contain confidential and privileged
} information.  If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination, distribution
} or copying of this message (including any attachments) is strictly
} prohibited.
}
} If you have received this message in error, please contact
} the sender by reply e-mail message and destroy all copies of the
} original message (including attachments).
}
}
} ==============================Original Headers==============================
} 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009
} 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94])
} 7, 32 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0M5U5Rv023498
} 7, 32 --     for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600
} 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net
} (emoryfloatdmz.cc.emory.edu [170.140.52.254])
} 7, 32 --     by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756
} 7, 32 --     for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500
} 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by
} 7, 32 --  EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP
} Server
} 7, 32 --  (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500
} 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
} 7, 32 --  EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22
} Jan 2009
} 7, 32 --  00:29:58 -0500
} 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500
} 7, 32 -- Subject: Histo diamond knife
} 7, 32 -- Thread-Topic: Histo diamond knife
} 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ==
} 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu}
} 7, 32 -- Accept-Language: en-US
} 7, 32 -- Content-Language: en
} 7, 32 -- X-MS-Has-Attach:
} 7, 32 -- X-MS-TNEF-Correlator:
} 7, 32 -- acceptlanguage: en-US
} 7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
} 7, 32 -- MIME-Version: 1.0
} 7, 32 -- X-emory.edu-MailScanner: Found to be clean
} 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
} 7, 32 -- X-Spam-Status: No
} 7, 32 -- Content-Transfer-Encoding: 8bit
} 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n0M5U5Rv023498
} ==============================End of - Headers==============================
}
}
}
}
}
}
} ==============================Original Headers==============================
} 22, 22 -- From nizets2-at-yahoo.com Thu Jan 22 02:41:24 2009
} 22, 22 -- Received: from web110805.mail.gq1.yahoo.com
} (web110805.mail.gq1.yahoo.com [67.195.13.228])
} 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n0M8fO8J012980
} 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 02:41:24 -0600
} 22, 22 -- Received: (qmail 62862 invoked by uid 60001); 22 Jan 2009 08:41:23
} -0000
} 22, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 22, 22 -- s=s1024; d=yahoo.com;
} 22, 22 --
} h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:C
} ontent-Type:Content-Transfer-Encoding:Message-ID;
} 22, 22 --
} b=arER5TCHEvR9cLCGy/Stts2255pSPF+6wxsQAw5DCTUcTxQvNs4cibc6EFv+GwnvxHoCBYwRbkyn
} 7DxspB5qSxEjMf5rV+boK90JSFsUlFw9b3w/LGbsrLdyy0goNk4HGLfs9mH0CixEVfqBLjPlFDmafb
} qbAdhEYQ8pH5avEvQ=;
} 22, 22 -- X-YMail-OSG:
} 98.knsQVM1n7xyKC0afwsbtGUfk4_PDhvvFu_Xs.c_vWUIPV.VfRtFQjDBQRBez96A4u5S.icydUpB
} r.GBpx.UeMFtxK1j6KAIaPJSSQcvAc17ghYIcQp3Z5bRsOW03oeiZYAKQ8arb1moDp5RosesU1lpvi
} frHF_FbFIsBu7f.TTn0Z4O_uC6VdNHvNjGkl27vz1kMLBxUV4c9jIzVtAgja4CoF1Qh7
} 22, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via
} HTTP; Thu, 22 Jan 2009 00:41:23 PST
} 22, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1
} 22, 22 -- References: {200901220534.n0M5Ybmx030096-at-ns.microscopy.com}
} 22, 22 -- Date: Thu, 22 Jan 2009 00:41:23 -0800 (PST)
} 22, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 22, 22 -- Subject: Re: [Microscopy] Histo diamond knife
} 22, 22 -- To: microscopy-at-microscopy.com
} 22, 22 -- MIME-Version: 1.0
} 22, 22 -- Content-Type: text/plain; charset=iso-8859-1
} 22, 22 -- Message-ID: {897590.62667.qm-at-web110805.mail.gq1.yahoo.com}
} 22, 22 -- Content-Transfer-Encoding: 8bit
} 22, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n0M8fO8J012980
} ==============================End of - Headers==============================




==============================Original Headers==============================
8, 45 -- From prvs=Rosemary.White=2668ccc91-at-csiro.au Thu Jan 22 14:53:53 2009
8, 45 -- Received: from vic-MTAout5.csiro.au (vic-MTAout5.csiro.au [150.229.64.42])
8, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MKrpNA001357
8, 45 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 14:53:52 -0600
8, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
8, 45 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
8, 45 -- s=email; t=1232657632; x=1264193632;
8, 45 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
8, 45 -- mime-version:content-transfer-encoding:content-id:
8, 45 -- content-description:resent-date:resent-from:resent-sender:
8, 45 -- resent-to:resent-cc:resent-message-id:in-reply-to:
8, 45 -- references:list-id:list-help:list-unsubscribe:
8, 45 -- list-subscribe:list-post:list-owner:list-archive;
8, 45 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
8, 45 -- |Subject:=20Re:=20[Microscopy]=20Re:=20Histo=20diamond=20
8, 45 -- knife|Date:=20Fri,=2023=20Jan=202009=2007:53:46=20+1100
8, 45 -- |Message-ID:=20 {C59F280A.2D41%rosemary.white-at-csiro.au}
8, 45 -- |To:=20 {Microscopy-at-microscopy.com} |MIME-Version:=201.0
8, 45 -- |Content-Transfer-Encoding:=20quoted-printable
8, 45 -- |In-Reply-To:=20 {200901220847.n0M8leM2022192-at-ns.microscop
8, 45 -- y.com} ;
8, 45 -- bh=HkhibgjilpmKit7sowfmymVmJ9+puoGXlZglRnsaQTs=;
8, 45 -- b=KRmXnAVvHKJb08akpKyw7h04fcdBhAyZfS0xlMgAJ2qVEAXgzwGeM4gT
8, 45 -- 3q+ed9s6AJRVZb5VgNPrC/xm5d/ghAhxjWF4rd6AmWLtjr49Qmqf/7hvk
8, 45 -- yf7lfqlBWjEyd1m;
8, 45 -- X-IronPort-AV: E=Sophos;i="4.37,308,1231074000";
8, 45 -- d="scan'208";a="7817743"
8, 45 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
8, 45 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 23 Jan 2009 07:53:49 +1100
8, 45 -- Received: from [152.83.167.123] (152.83.167.123) by
8, 45 -- EXNSW-HTCA01.nexus.csiro.au (130.155.117.126) with Microsoft SMTP Server id
8, 45 -- 8.1.311.2; Fri, 23 Jan 2009 07:53:49 +1100
8, 45 -- User-Agent: Microsoft-Entourage/12.10.0.080409
8, 45 -- Date: Fri, 23 Jan 2009 07:53:46 +1100
8, 45 -- Subject: Re: [Microscopy] Re: Histo diamond knife
8, 45 -- From: Rosemary White {rosemary.white-at-csiro.au}
8, 45 -- To: {Microscopy-at-microscopy.com}
8, 45 -- Message-ID: {C59F280A.2D41%rosemary.white-at-csiro.au}
8, 45 -- Thread-Topic: [Microscopy] Re: Histo diamond knife
8, 45 -- Thread-Index: Acl8bhg2qF/jtdUcSk+BEhIpq8eDHgAZWxNq
8, 45 -- In-Reply-To: {200901220847.n0M8leM2022192-at-ns.microscopy.com}
8, 45 -- MIME-Version: 1.0
8, 45 -- Content-Type: text/plain; charset="ISO-8859-1"
8, 45 -- Content-Transfer-Encoding: 8bit
8, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MKrpNA001357
==============================End of - Headers==============================




From: lemon-at-email.arizona.edu
Date: Thu, 22 Jan 2009 15:25:43 -0600
Subject: [Microscopy] Sapphire and Zirconia cover slips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Does anyone know where I can buy zirconia or sapphire (alumina) coverslips
(~0.15mm thick)? I have been unable to find a vendor for these items.

Thank you,

John Lemon
Graduate Student
The University of Arizona
Department of Chemistry


==============================Original Headers==============================
5, 25 -- From lemon-at-email.arizona.edu Thu Jan 22 15:25:41 2009
5, 25 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MLPfiG022083
5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 15:25:41 -0600
5, 25 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238])
5, 25 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id DAC499A01AF
5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 14:25:40 -0700 (MST)
5, 25 -- Received: from localhost (matador.email.arizona.edu [10.0.0.218])
5, 25 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4DFDB9A0417
5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 14:25:40 -0700 (MST)
5, 25 -- Received: from 128.196.212.128 ([128.196.212.128]) by www.email.arizona.edu
5, 25 -- (Horde) with HTTP for {lemon-at-email.arizona.edu} ; Thu, 22 Jan 2009 14:25:40
5, 25 -- -0700
5, 25 -- Message-ID: {20090122142540.3tetno8ogk8owkc4-at-www.email.arizona.edu}
5, 25 -- X-Priority: 3 (Normal)
5, 25 -- Date: Thu, 22 Jan 2009 14:25:40 -0700
5, 25 -- From: lemon-at-email.arizona.edu
5, 25 -- To: Microscopy-at-microscopy.com
5, 25 -- Subject: Sapphire and Zirconia cover slips
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Type: text/plain; charset="ISO-8859-1"
5, 25 -- Content-Disposition: inline
5, 25 -- Content-Transfer-Encoding: 7bit
5, 25 -- User-Agent: Internet Messaging Program (IMP) 4.0-cvs
5, 25 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 22 Jan 2009 17:00:32 -0600
Subject: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote:

} I have been working with a researcher here looking at some TiO2
} Nanotubes in the TEM. The researcher wants to see if his nanotubes
} are hollow or not. We are looking for a core of around 5 nm.
}
} Being a biology lab we do not have any experience with such samples.
} I have tried dusting the fragments (provided in powder form from
} scrappings off a Ti plate) onto carbon/formvar grids and I have
} tried drying them onto a grid from a suspension in ethanol. The
} solvent method proved more successful at getting particles onto the
} film than the dusting method however in both cases they tend to be
} clumped.
}
} The problem I have is the clumps must be heating up in the beam. I
} as soon as I start to increase the magnification to explore the edges
} the sample disappears and I am left with a hole in the film.
}
} I am pushing a 100kV instrument so I suspect this may have something
} to with it also.
}
} A search of the literature indicates that a lot of people are
} investigating TiO2 nanotubes in the TEM but nothing I have found so
} far talks about how the nanotubes are got onto a grid in a usable
} form to image. lots of info about making nanotubes.
}
} Any thoughts or suggestions would be appreciated.


Dear Allan,
I have not had experience with TiO2 nanotubes, but I do have a couple
of suggestions. Since the EtOH suspension method seems to work better
than dusting, but still results in clumping, you might try a more
volatile solvent, or applying the suspension in a higher-temperature
environment, such as a warm room. Faster evaporation of the solvent
should reduce clumping. I suspect that it is charging of the
nanotubes, rather than heating, that is causing problems. Especially
if you are using a slot grid, charge buildup on the film can cause it
to break. I suggest evaporating a layer of carbon onto the grid
before trying to look it, and be sure that the objective aperture is
inserted--backscattering from the aperture can neutralize some of the
built-up charge. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009
6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170
6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600
6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC;
6, 23 -- Thu, 22 Jan 2009 15:00:30 -0800 (PST)
6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F;
6, 23 -- Thu, 22 Jan 2009 15:00:29 -0800 (PST)
6, 23 -- Cc: microscopy-at-microscopy.com
6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu}
6, 23 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz
6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 23 -- Content-Transfer-Encoding: 7bit
6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes
6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800
6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
6, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: r-holdford-at-ti.com
Date: Thu, 22 Jan 2009 18:03:06 -0600
Subject: [Microscopy] LaB6 problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan: Bill is correct. It's been years since I ran a Kimball LaB6 in
an SEM (or any other thermionic tip having gone the FE route) but
Kimball tips need higher saturation currents than other manufacturers.
I don't remember if I even had numbers on my saturation knob, but as
Bill says, run it up to saturation and use it there.

tivol-at-caltech.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} On Jan 22, 2009, at 11:26 AM, stefan.diller-at-t-online.de wrote:
}
}
} } since some weeks I am trying to bring a new Kimball LaB6 cathode type
} } 423E90 up to performance.
} } I am using the cathode in a Philips 525 SEM.
} } My experience with the cathodes before had been that the heating
} } current
} } had been with the old cathodes at 11 to 13 max. The new cathode
} } needs to
} } have 14 to 16 max.
} } I am using the LaB6 wehnelt cap and set the height (with a 0.5mm
} } wehnelt
} } aperture) at ca. 0,25mm down from the surface of the aperture disc.
} }
} } Please have a look at the images:
} } www.elektronenmikroskopie.info/lab6
} }
} } With heating current at position 11 I am still getting double contours
} } in the image (LaB6_3.jpg), which is getting better when I am using a
} } smaller spotsize (LaB6_2.jpg).
} } With heating current at position 14 I still have some "feeling" of
} } double contours in the image (see LaB6_4.jpg), which disapears at
} } smaller spotsize (LaB6_5.jpg).
} } Best image so far is LaB6_8.jpg at 10nm spotsize and heating current
} } position of 16 (which seems for me to be too far up the scale).
} }
} } ...I put the cathode in my EM420 TEM and looked at the cathode image
} } (I
} } am not able to do this in the SEM...).
} } At high beam current and at heating current position ca. 12 (which
} } is 2
} } steps more than on older cathodes) the flat tip of the cathode
} } showed up
} } nicely, resembling a "cross" and comes to an even illumination at
} } position 14.
} }
} } My question is:
} } Is anybody out there giving me some tips if heating values might be
} } correct? Is there a problem with the wehnelt distance?
} } I never experienced such a behavior before...
} } Is there a chance of cathode charging or an inappropriate value of the
} } wehnelt voltage? I changed wehnelt values, with no better imaging.
} }
}
}
} Dear Stefan,
} If you are comparing the Kimball LaB6 to another brand, then it is
} not too surprising that the heating currents are different. Kimball
} mounts the LaB6 crystal in a cup, which is heated and transfers the
} heat to the filament; whereas, some other brands allow the current to
} go through the LaB6 directly. There may well be differences in heat
} transfer that require a higher current for the Kimball. In any event,
} it is best to operate with the filament saturated (assuming that you
} are not interested in operating in tip mode, where only the flat of
} the filament emits electrons). If the higher current required makes
} the life of the tip too short, then you may want to use a different
} brand of tip; however, our experience with Kimball LaB6 tips has been
} quite good. Our experience may not be too good a guide, since we have
} only TEMs, but I think the requirements for good performance are
} pretty much the same--high brightness and good coherence.
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
} ==============================Original Headers==============================
} 6, 22 -- From tivol-at-caltech.edu Thu Jan 22 13:54:12 2009
} 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
} 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MJsBV2014495
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 13:54:11 -0600
} 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
} 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 2E911329E5E
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:11 -0800 (PST)
} 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
} 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
} 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id CEC88328F6E
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:09 -0800 (PST)
} 6, 22 -- Message-Id: {6705092A-D812-4998-B81C-0596D24E8071-at-caltech.edu}
} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
} 6, 22 -- To: microscopy-at-microscopy.com
} 6, 22 -- In-Reply-To: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com}
} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 6, 22 -- Content-Transfer-Encoding: 7bit
} 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 6, 22 -- Subject: Re: [Microscopy] LaB6 problems
} 6, 22 -- Date: Thu, 22 Jan 2009 11:54:09 -0800
} 6, 22 -- References: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com}
} 6, 22 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================
}
}
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA
Texas Instruments, Inc.
13536 N. Central Expressway MS 940
Dallas, TX 75243
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
4, 22 -- From r-holdford-at-ti.com Thu Jan 22 18:03:05 2009
4, 22 -- Received: from comal.ext.ti.com (comal.ext.ti.com [198.47.26.152])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N034TR004877
4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 18:03:05 -0600
4, 22 -- Received: from dlep33.itg.ti.com ([157.170.170.112])
4, 22 -- by comal.ext.ti.com (8.13.7/8.13.7) with ESMTP id n0N02vp3015082;
4, 22 -- Thu, 22 Jan 2009 18:03:02 -0600
4, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1])
4, 22 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id n0N02vIS001360;
4, 22 -- Thu, 22 Jan 2009 18:02:57 -0600 (CST)
4, 22 -- Message-ID: {49790931.4090402-at-ti.com}
4, 22 -- Date: Thu, 22 Jan 2009 18:02:57 -0600
4, 22 -- From: Becky Holdford {r-holdford-at-ti.com}
4, 22 -- Organization: SC Packaging Development -- FA Development
4, 22 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
4, 22 -- MIME-Version: 1.0
4, 22 -- To: stefan.diller-at-t-online.de, MSA Listserver {Microscopy-at-microscopy.com}
4, 22 -- Subject: Re: [Microscopy] Re: LaB6 problems
4, 22 -- References: {200901221954.n0MJsOfh014754-at-ns.microscopy.com}
4, 22 -- In-Reply-To: {200901221954.n0MJsOfh014754-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 22 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 22 Jan 2009 18:51:18 -0600
Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The method below was sent to the list as a method to solve the same type
of problem in the context of SEM, but it should also work for TEM
samples on a carbon film. Echoes of the ethernet - old friends come back
to visit.

Dale

Sent by } Henk Colijn
} colijn.1-at-osu.edu
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Oldrich Benada wrote:
} =====================================================
} I need some advice. I was asked to do some analysis of silica particles
} (size distribution) for chemist in our institute. Particle size should be in
} the range of 3 to 6 um. I do not have any experiences with such sample.
} Could someone give me a tip how to prepare sample for TEM (or SEM)?
} ======================================================
}
} The problem is that those pesky silica particles don't know that they are
} supposed to separate and stay away from each other when dispersed in a
} liquid followed by a droplet of this liquid suspension being placed on a
} solid surface. They tend to agglomerate very quickly leading to a difficult-
} to-analyze situation, especially using automated means of analysis. You are
} correct in that the size range expected could be on the order of 3-6 nm.
}
} This is the ideal application for the camphor/naphthalene method which I
} described several years ago. Credit for the technique, or at least the one
} who taught it to me was an innovative microscopist then working at the
} DuPont Experimental Station in Wilmington, DE by the name of Robert P.
} Schatz, in about 1968, now deceased. Take a 60% camphor/40% naphthalene
} mixture and heat it to twenty or so degrees above room temperature on a hot
} plate in a small beaker or flask, the two organics are miscible in each
} other and this is the eutectic composition.
}
} Once a clear liquid, add a small amount of the silica (not more than 0.1%),
} which disperses quite readily. Then, using a pipette, take out some liquid
} and put a drop onto a carbon coated glass slide, at which time the drop is
} instantly frozen solid (it is at room temperature). Put the slide into your
} vacuum evaporator to pump out all night, and the "magic" is that the solid
} eutectic sublimes at room temperature at a rate that by morning, it is
} completely gone, leaving the silica particles uniformly dispersed on the
} carbon film!
}
} The rest is obvious. You can pick this up on a grid, as is, or in order to
} bring out more contrast, Pt/C shadow, probably using an angle not more than
} 30 degrees. You can float the "replica" off of the slide directly onto a
} grid and viola! you have particles completely dispersed, virtually no
} doublets or triplets, and a field quite amenable for automated image
} analysis (as a bonus).
}
} One important further suggestion: Some times these silica particles tend to
} fuse together as little "chains". If you suspect this is happening, be sure
} to take the micrographs as stereo pairs because you can in fact capture this
} three dimensional spatial information.
}
} Disclaimer: We do not sell either the camphor or naphthalene so have no
} vested interest in whether people use this method or not. It is just a
} really neat method for the preparation of fine particle samples in this size
} range. We are obviously set up to use this method as a service for others,
} however.
}
} Chuck




tivol-at-caltech.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote:
}
} } I have been working with a researcher here looking at some TiO2
} } Nanotubes in the TEM. The researcher wants to see if his nanotubes
} } are hollow or not. We are looking for a core of around 5 nm.
} }
} } Being a biology lab we do not have any experience with such samples.
} } I have tried dusting the fragments (provided in powder form from
} } scrappings off a Ti plate) onto carbon/formvar grids and I have
} } tried drying them onto a grid from a suspension in ethanol. The
} } solvent method proved more successful at getting particles onto the
} } film than the dusting method however in both cases they tend to be
} } clumped.
} }
} } The problem I have is the clumps must be heating up in the beam. I
} } as soon as I start to increase the magnification to explore the edges
} } the sample disappears and I am left with a hole in the film.
} }
} } I am pushing a 100kV instrument so I suspect this may have something
} } to with it also.
} }
} } A search of the literature indicates that a lot of people are
} } investigating TiO2 nanotubes in the TEM but nothing I have found so
} } far talks about how the nanotubes are got onto a grid in a usable
} } form to image. lots of info about making nanotubes.
} }
} } Any thoughts or suggestions would be appreciated.
}
}
} Dear Allan,
} I have not had experience with TiO2 nanotubes, but I do have a couple
} of suggestions. Since the EtOH suspension method seems to work better
} than dusting, but still results in clumping, you might try a more
} volatile solvent, or applying the suspension in a higher-temperature
} environment, such as a warm room. Faster evaporation of the solvent
} should reduce clumping. I suspect that it is charging of the
} nanotubes, rather than heating, that is causing problems. Especially
} if you are using a slot grid, charge buildup on the film can cause it
} to break. I suggest evaporating a layer of carbon onto the grid
} before trying to look it, and be sure that the objective aperture is
} inserted--backscattering from the aperture can neutralize some of the
} built-up charge. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
} ==============================Original Headers==============================
} 6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009
} 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
} 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170
} 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600
} 6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
} 6, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC;
} 6, 23 -- Thu, 22 Jan 2009 15:00:30 -0800 (PST)
} 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
} 6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
} 6, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F;
} 6, 23 -- Thu, 22 Jan 2009 15:00:29 -0800 (PST)
} 6, 23 -- Cc: microscopy-at-microscopy.com
} 6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu}
} 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu}
} 6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz
} 6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 6, 23 -- Content-Transfer-Encoding: 7bit
} 6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes
} 6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800
} 6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
} 6, 23 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================

==============================Original Headers==============================
7, 20 -- From dac-at-research.umass.edu Thu Jan 22 18:51:17 2009
7, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N0pHJ5019356
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 18:51:17 -0600
7, 20 -- Received: from [192.168.1.103] (static.unknown.charter.com [96.39.6.64] (may be forged))
7, 20 -- (authenticated bits=0)
7, 20 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0N0pGdN004141
7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 19:51:16 -0500
7, 20 -- Message-ID: {497914BD.4020505-at-research.umass.edu}
7, 20 -- Date: Thu, 22 Jan 2009 19:52:13 -0500
7, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
7, 20 -- MIME-Version: 1.0
7, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes
7, 20 -- References: {200901222307.n0MN7LOl030357-at-ns.microscopy.com}
7, 20 -- In-Reply-To: {200901222307.n0MN7LOl030357-at-ns.microscopy.com}
7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Fri, 23 Jan 2009 02:51:15 -0600
Subject: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Allan!

I second all the suggestions of Bill, which you really should try.
I would like to add some suggestions.
Take carbon-coated grids (without formvar, or dissolve formvar from your grids). Heat the grids themselves and deposit a drop of your suspension (in methanol f.ex) on the grid, the solvent will evaporate immediately.
Now one possility is that the nanotubes are already clumped in solution. In this case perhaps you can ultrasonicate them. I don't know if the treatment may be deleterious to the structure, but if try you'll know.
Perhaps you start with agglomerated nanotubes. If you just want to see the internal core, I suppose you don't mind about breaking the tubes. In this case you may want to crush them in a mill or in a mortar to obtain a fine powder.
And, last but not least: just sort the powder yourself! By centrifuging the suspension, you'll pellet the bigger agglomerates and keep the finest powder in suspension. You can do it in ethanol. You'll just have to adjust the parameters to reach the optimal sorting for your needs.  I suspect that centrifuging at 200g for 30 min would keep particulates of approx. 500 nm in suspension.

Best regards,

Stephane

PS: not sure about it, but in case post-coating with carbon does not suffice, I wouldn't be surprised if thin coating with gold would prevent charging without much disturbing the primary electrons.

 


----- Original Message ----
X-from: "tivol-at-caltech.edu" {tivol-at-caltech.edu}
To: nizets2-at-yahoo.com
Sent: Friday, January 23, 2009 12:06:18 AM


On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote:

} I have been working with a researcher here looking at some TiO2
} Nanotubes in the TEM.  The researcher wants to see if his nanotubes
} are hollow or not.  We are looking for a core of around 5 nm.
}
} Being a biology lab we do not have any experience with such samples.
} I have tried dusting the fragments (provided in powder form from
} scrappings off a Ti plate) onto  carbon/formvar grids and I have
} tried drying them onto a grid from a suspension in ethanol.  The
} solvent method proved more successful at getting particles onto the
} film than the dusting method however in both cases they tend to be
} clumped.
}
} The problem I have is the clumps must be heating up in the beam.  I
} as soon as I start to increase the magnification to explore the edges
} the sample disappears and I am left with a hole in the film.
}
} I am pushing a 100kV instrument so I suspect this may have something
} to with it also.
}
} A search of the literature indicates that a lot of people are
} investigating  TiO2 nanotubes in the TEM but nothing I have found so
} far talks about how the nanotubes are got onto a grid in a usable
} form to image.  lots of info about making nanotubes.
}
} Any thoughts or suggestions would be appreciated.


Dear Allan,
    I have not had experience with TiO2 nanotubes, but I do have a couple 
of suggestions.  Since the EtOH suspension method seems to work better 
than dusting, but still results in clumping, you might try a more 
volatile solvent, or applying the suspension in a higher-temperature 
environment, such as a warm room.  Faster evaporation of the solvent 
should reduce clumping.  I suspect that it is charging of the 
nanotubes, rather than heating, that is causing problems.  Especially 
if you are using a slot grid, charge buildup on the film can cause it 
to break.  I suggest evaporating a layer of carbon onto the grid 
before trying to look it, and be sure that the objective aperture is 
inserted--backscattering from the aperture can neutralize some of the 
built-up charge.  Good luck.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009
6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170
6, 23 --     for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600
6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 23 --     by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC;
6, 23 --     Thu, 22 Jan 2009 15:00:30 -0800 (PST)
6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 23 --     by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F;
6, 23 --     Thu, 22 Jan 2009 15:00:29 -0800 (PST)
6, 23 -- Cc: microscopy-at-microscopy.com
6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu}
6, 23 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz
6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 23 -- Content-Transfer-Encoding: 7bit
6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes
6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800
6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com}
6, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================






==============================Original Headers==============================
23, 23 -- From nizets2-at-yahoo.com Fri Jan 23 02:51:14 2009
23, 23 -- Received: from web110803.mail.gq1.yahoo.com (web110803.mail.gq1.yahoo.com [67.195.13.226])
23, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0N8pDoE015170
23, 23 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 02:51:14 -0600
23, 23 -- Received: (qmail 1088 invoked by uid 60001); 23 Jan 2009 08:51:13 -0000
23, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 23 -- s=s1024; d=yahoo.com;
23, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
23, 23 -- b=d7Yg7HjoJDFJDDnuPeBZ0EWxSIekKoXRF7kvph17C9cxKji57Ol7UeNO5wX0lNkZw/Q8hsXQLwT1vheBcJ/0euB76RKWWqPM12zN8CVuOwroq4r47J583aBlRuBZtW1CoQSL+6hQINvM+Z/3HcKfbLnCSk+rjWOGEtCsd8EZDlg=;
23, 23 -- X-YMail-OSG: S2IVPL4VM1mcckkpPaikPtjfhOR6JNgirF8DNcROOHE2Hzwgnj.WWTwApHKOtDAmGgMU3vNvlEsVsazHnEmIWSiCes8fFm6KdsycqOlRcPxCCdPqvjfRN5QQ9_F.C6FIfg555X52zMuBuLg3bH9KiJDoK9uwIhjb39VzgvOR.M8ZNJXJ1yLYMMPhmZMytHq8vy3V3CXIjo0VO2e_OaRLNovA.LQ6I8ql
23, 23 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Fri, 23 Jan 2009 00:51:13 PST
23, 23 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1
23, 23 -- References: {200901222306.n0MN6IeD028777-at-ns.microscopy.com}
23, 23 -- Date: Fri, 23 Jan 2009 00:51:13 -0800 (PST)
23, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 23 -- Subject: Re: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes
23, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz
23, 23 -- Cc: microscopy-at-microscopy.com
23, 23 -- MIME-Version: 1.0
23, 23 -- Content-Type: text/plain; charset=iso-8859-1
23, 23 -- Message-ID: {461322.852.qm-at-web110803.mail.gq1.yahoo.com}
23, 23 -- Content-Transfer-Encoding: 8bit
23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0N8pDoE015170
==============================End of - Headers==============================




From: elena.belluso-at-unito.it
Date: Fri, 23 Jan 2009 03:58:54 -0600
Subject: [Microscopy] ICC asbestos session

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are pleased to invite you to submit an abstract to session HE1 at the
14th International Clay Conference scheduled in June 14th-20th 2009 in
Castellaneta Marina, near Bari, in Southeast Italy =
(http://www.14icc.org/ ).

The abstract deadline has been extended to January 31.


Please note that contributions to 14th International Clay Conference may =
be submitted for publication in a special issue of Applied Clay Science. =
They will pass the usual peer review process and the issue is expected to
be published before the end of 2010.

With ours best wishes for the New Year, the convenors Elena Belluso
(elena.belluso-at-unito.it) and Mickey Gunter(mgunter-at-uidaho.edu)


Session HE1 "Asbestos Monitoring & Analytical Methods"

Monitoring, identification, and quantification of asbestos are essential
aspects of dealing with these minerals. These investigations are very
important to the regulatory community because special precautions must =
be taken when asbestos is found in significant amounts.

Different techniques of monitoring and analysis are necessary depending =
on where the asbestos occurs: air, water, soils, rocks, biological =
materials, asbestos-containing materials and their transformation
products.

Besides, for asbestos use in health-based studies it is important to =
apply several complementary analytical methods.

This session will: 1) present the state of the art in monitoring and
techniques actually considered the most suitable for the different =
asbestos containing materials; 2) compare various investigation protocols;
3) exchange information about the advances in this topic; and 4) develop
interdisciplinary collaborations.

Convenors: Elena Belluso (elena.belluso-at-unito.it) and Mickey Gunter
(mgunter-at-uidaho.edu)


-----------------------------------------------------------------------------------
Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography
Dipartimento di Scienze Mineralogiche e Petrologiche
Universita' degli Studi di Torino
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28
e-mail: elena.belluso-at-unito.it
http://www.dsmp.unito.it
-----------------------------------------------------------------------------------
"I've... seen things you people wouldn't believe.
Attack ships on fire off the shoulder of Orion.
I watched C-beams... glitter in the dark near the Tanhauser Gate.
All those... moments will be lost... in time...,
like... tears... in... rain."
Blade Runner




==============================Original Headers==============================
18, 29 -- From elena.belluso-at-unito.it Fri Jan 23 03:58:54 2009
18, 29 -- Received: from mail-out.unito.it (opterone.unito.it [130.192.119.88])
18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N9wrhx030275
18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 03:58:54 -0600
18, 29 -- Received: (from root-at-localhost)
18, 29 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) id n0N9wrKt026227
18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 10:58:53 +0100
18, 29 -- Received: from mail.unito.it (giove.unito.it [130.192.119.45])
18, 29 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) with SMTP id n0N9wpOK026202
18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 10:58:51 +0100
18, 29 -- X-Icontrol: Sent by Inrete Icontrol
18, 29 -- Received: from 130.192.111.68
18, 29 -- (SquirrelMail authenticated user ebelluso-at-unito.it)
18, 29 -- by mail.unito.it with HTTP;
18, 29 -- Fri, 23 Jan 2009 10:58:51 +0100 (CET)
18, 29 -- Message-ID: {1973.130.192.111.68.1232704731.squirrel-at-mail.unito.it}
18, 29 -- Date: Fri, 23 Jan 2009 10:58:51 +0100 (CET)
18, 29 -- Subject: ICC asbestos session
18, 29 -- From: elena.belluso-at-unito.it
18, 29 -- To: Microscopy-at-microscopy.com
18, 29 -- User-Agent: SquirrelMail/1.4.2
18, 29 -- MIME-Version: 1.0
18, 29 -- Content-Type: text/plain;charset=iso-8859-1
18, 29 -- Content-Transfer-Encoding: 8bit
18, 29 -- X-Priority: 3
18, 29 -- Importance: Normal
18, 29 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv
18, 29 -- X-Inrete-Amavisjob-Service-Runned: 6 (n0N9wpOK026202)
18, 29 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (n0N9wpOK026202)
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 23 Jan 2009 06:15:39 -0600
Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Allan,
I've had a number of users in the past looking at nanotubes here in
my lab, and one of them was interested in the internal microstructure. We
ended up embedding the nanotubes in resin and thin-sectioning them. It
worked pretty well, but the structure they were looking at was a little
grosser than your's. If you think it's worth a shot, let me know and I'll
dig out my files and find out exactly how we did it.
Cheers,
Jules

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
3, 23 -- From bafg3-at-sussex.ac.uk Fri Jan 23 06:15:38 2009
3, 23 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NCFc5d016333
3, 23 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 06:15:38 -0600
3, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:2710)
3, 23 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
3, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} )
3, 23 -- id KDXBIW-000J5M-5W
3, 23 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009 12:18:32 +0000
3, 23 -- Date: Fri, 23 Jan 2009 12:15:32 -0000
3, 23 -- To: microscopy-at-microscopy.com
3, 23 -- Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes
3, 23 -- Message-ID: {57318378.1232712932-at-ls0130.lifesci.susx.ac.uk}
3, 23 -- Originator-Info: login-token=Mulberry:01Tty6mrA9IAGCHBr0t0B8Ty8NwkElwa83fFEl;
3, 23 -- token_authority=postmaster-at-central.susx.ac.uk
3, 23 -- X-Mailer: Mulberry/2.0.8 (Win32)
3, 23 -- MIME-Version: 1.0
3, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed
3, 23 -- Content-Transfer-Encoding: 7bit
3, 23 -- Content-Disposition: inline
3, 23 -- X-Sussex: true
3, 23 -- X-Sussex-transport: remote_smtp_rew
3, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 23 Jan 2009 09:16:45 -0600
Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Re: TEM of TiO2 nanotubes

Dear Allan (and All),
Apologies, but I should have clarified that the nanotubes that I
embedded previously for thin-sectioning were carbon, not TiO2 (it's Friday
and am a bit tired - should have read the subject header more carefully!).
I have no experience of dealing with the latter, so could not say whether
the embedding route would work or not.
Cheers,
Jules

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
4, 23 -- From bafg3-at-sussex.ac.uk Fri Jan 23 09:16:45 2009
4, 23 -- Received: from sivits.uscs.susx.ac.uk (sivits.uscs.susx.ac.uk [139.184.14.88])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NFGixI004435
4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 09:16:45 -0600
4, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:1721)
4, 23 -- by sivits.uscs.susx.ac.uk with esmtp (Exim 4.64)
4, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} )
4, 23 -- id KDXJTO-0004X9-5S
4, 23 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009 15:17:48 +0000
4, 23 -- Date: Fri, 23 Jan 2009 15:16:43 -0000
4, 23 -- To: microscopy-at-microscopy.com
4, 23 -- Subject: [Microscopy] viaWWW: TEM of TiO2 nanotubes
4, 23 -- Message-ID: {68189610.1232723803-at-ls0130.lifesci.susx.ac.uk}
4, 23 -- Originator-Info: login-token=Mulberry:01OYPHtpvkLR1twycQoudn9jwpregy3ZreZ/gy;
4, 23 -- token_authority=postmaster-at-central.susx.ac.uk
4, 23 -- X-Mailer: Mulberry/2.0.8 (Win32)
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- Content-Disposition: inline
4, 23 -- X-Sussex: true
4, 23 -- X-Sussex-transport: remote_smtp_rew
4, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 23 Jan 2009 09:54:30 -0600
Subject: [Microscopy] TEM: TAAB Low Viscosity Resin Post-Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I've recently been using TAAB Low Viscosity (TLV) resin (since the
sad demise of Spurr resin!) for embedding standard double-fixed cells and
tissues and sometimes have problems achieving good contrast with routine
uranyl acetate (UA) and Pb citrate post-staining. Has anyone else
experienced the same problem and found a way to get over it? I guess
alcoholic UA might be the way to go, but not sure how you rinse the grids
after this.
Thanks in advance for any advice,
Julian

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
3, 23 -- From bafg3-at-sussex.ac.uk Fri Jan 23 09:54:29 2009
3, 23 -- Received: from sivits.uscs.susx.ac.uk (sivits.uscs.susx.ac.uk [139.184.14.88])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NFsSFU019603
3, 23 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 09:54:29 -0600
3, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:2025)
3, 23 -- by sivits.uscs.susx.ac.uk with esmtp (Exim 4.64)
3, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} )
3, 23 -- id KDXLKJ-000DOV-IR
3, 23 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009 15:55:31 +0000
3, 23 -- Date: Fri, 23 Jan 2009 15:54:26 -0000
3, 23 -- To: microscopy-at-microscopy.com
3, 23 -- Subject: TEM: TAAB Low Viscosity Resin Post-Staining
3, 23 -- Message-ID: {70453044.1232726066-at-ls0130.lifesci.susx.ac.uk}
3, 23 -- Originator-Info: login-token=Mulberry:01nI/Gpv7wLA3QzyYABfPKAjsZEPQxzf3qygQx;
3, 23 -- token_authority=postmaster-at-central.susx.ac.uk
3, 23 -- X-Mailer: Mulberry/2.0.8 (Win32)
3, 23 -- MIME-Version: 1.0
3, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed
3, 23 -- Content-Transfer-Encoding: 7bit
3, 23 -- Content-Disposition: inline
3, 23 -- X-Sussex: true
3, 23 -- X-Sussex-transport: remote_smtp_rew
3, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 23 Jan 2009 09:57:50 -0600
Subject: [Microscopy] Biological TEM: TAAB Low Viscosity (TLV) resin-embedded specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I've recently been using TAAB Low Viscosity (TLV) resin (since the
sad demise of Spurr resin!) for embedding standard double-fixed cells and
tissues and sometimes have problems achieving good contrast with routine
uranyl acetate (UA) and Pb citrate post-staining. Has anyone else
experienced the same problem and found a way to get over it? I guess
alcoholic UA might be the way to go, but not sure how you rinse the grids
after this.
Thanks in advance for any advice,
Julian

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
3, 24 -- From bafg3-at-sussex.ac.uk Fri Jan 23 09:57:50 2009
3, 24 -- Received: from sivits.uscs.susx.ac.uk (sivits.uscs.susx.ac.uk [139.184.14.88])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NFvnrr023009
3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 09:57:49 -0600
3, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:2132)
3, 24 -- by sivits.uscs.susx.ac.uk with esmtp (Exim 4.64)
3, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
3, 24 -- id KDXLQ4-000E70-GX
3, 24 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009 15:58:52 +0000
3, 24 -- Date: Fri, 23 Jan 2009 15:57:47 -0000
3, 24 -- To: microscopy-at-microscopy.com
3, 24 -- Subject: Biological TEM: TAAB Low Viscosity (TLV) resin-embedded specimen
3, 24 -- section post-staining
3, 24 -- Message-ID: {70653603.1232726267-at-ls0130.lifesci.susx.ac.uk}
3, 24 -- Originator-Info: login-token=Mulberry:01R+B5y6lB3zJ7INklsER1U+4+u0Xk8qg7dVXk;
3, 24 -- token_authority=postmaster-at-central.susx.ac.uk
3, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- Content-Disposition: inline
3, 24 -- X-Sussex: true
3, 24 -- X-Sussex-transport: remote_smtp_rew
3, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Fri, 23 Jan 2009 10:06:56 -0600
Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dr. Besenyo:

Thank you! Thank you very very much! For explaining where the
terminology "Depleted" comes from - I know that a number of us have
been wondering that for a long time.

As well as taking the time to explain the general process for the
manufacture of our Uranyl acetate.


On 8 Jan 2009 at 19:20, abesenyo-at-ibilabs.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: abesenyo-at-ibilabs.com
} Name: Alex Besenyo PhD
}
} Organization: ibilabs
}
} Title-Subject: [Filtered] uranyl compounds are alpha emitters only
}
} Question: Question:
}
} Is it true that the stuff we use has been somehow
} depleted, so that it isn't as radioactive as "real" uranyl
} salts? Or is this yet another old wive's tale of EM?!
}
} Reply:
}
} When we manufacture these compounds we purchase the raw uranium in a
} depleted state from the government. There is no chance for error
} here. We do not use natural uranium.
}
} This means that the enrichable uranium U-235 has been removed.
} The then U-238 which only emitts alpha radiation is procesed.
}
} The term "depleted" means that U-235 has been removed.
}
} If even by the slightest chance that U235 were present then every
} alarm would go off in our facility because Beta and Gamma radiation
} is detected.
}
} I hope this answers everybodies concerns.
}
} Our products are sold exclusively through a distributor network and
} all of them have been instructed on this information.
}
} I only responded when I saw the original post and I had to respond
} before it got out of control.
}
} Sincerely
} Alex Besenyo PhD
}
}
} Login Host: 74.173.69.139
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009
} 17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
} 17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n090Jp6S032218
} 17, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52 -0600
} 17, 11 -- Mime-Version: 1.0
} 17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]}
} 17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600
} 17, 11 -- To: microscopy-at-microscopy.com
} 17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver)
} 17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only
} 17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


==============================Original Headers==============================
10, 25 -- From edelmare-at-muohio.edu Fri Jan 23 10:06:56 2009
10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.69])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NG6tDK014674
10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 23 Jan 2009 10:06:55 -0600
10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0NG6v7U000367;
10, 25 -- Fri, 23 Jan 2009 11:06:57 -0500
10, 25 -- Received: from [192.168.1.23] ([134.53.14.105])
10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0NG6j0H031024;
10, 25 -- Fri, 23 Jan 2009 11:06:45 -0500
10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
10, 25 -- To: "abesenyo-at-ibilabs.com" {abesenyo-at-ibilabs.com}
10, 25 -- Date: Fri, 23 Jan 2009 11:06:44 -0500
10, 25 -- MIME-Version: 1.0
10, 25 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters only
10, 25 -- CC: microscopy-at-Microscopy.com
10, 25 -- Message-ID: {4979A4C4.10991.5D62DA3C-at-edelmare.muohio.edu}
10, 25 -- Priority: normal
10, 25 -- In-reply-to: {200901090020.n090KkVO001188-at-ns.microscopy.com}
10, 25 -- References: {200901090020.n090KkVO001188-at-ns.microscopy.com}
10, 25 -- X-mailer: Pegasus Mail for Windows (4.41)
10, 25 -- Content-type: text/plain; charset=US-ASCII
10, 25 -- Content-transfer-encoding: 7BIT
10, 25 -- Content-description: Mail message body
10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Fri, 23 Jan 2009 11:51:56 -0600
Subject: [Microscopy] Fwd: Biological TEM: TAAB Low Viscosity (TLV) resin-embedded specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Julian,

I haven't used TAAB but have experience with Spurr and the recent low
viscosity epoxy sold in the US by Electron Microscopy Sciences. Both
are harder to stain than either "normal", Epon/Araldite like epoxies
or acrylics. The reason is the higher degree of cross-linking.

(Not something you are asking, but do you really need to use a low
viscosity resin? Besides the staining issues, they are also more of a
health hazard.)

Yes, alcoholic UA will stain better, and you are correct, some caution
is needed when rinsing afterwards. My favorite UA procedure for such
cases is to use the stock of saturated UA in water and dilute it
before each staining 1:1 with methanol. This way you don't need to
worry about precipitate and still have high enough concentration of UA
in 50% methanol, fresh each time. Stain for 5-10 minutes. I rinse
first on a drop of 50% methanol, then 25% methanol, then a few
droplets of water gently flowing from a disposable 3 ml pipette. It is
a good general practice to avoid overwashing, both with UA and Pb
staining. Too much washing won't get rid of the precipitate formed on
sections but will destain.

Whenever I have such contrast issues, I also use Venable-Coggeshall
[spelling?] recipe for the lead stain. I keep pre-weighed amounts in
15 ml Falcon tubes and make it fresh every such time (1-2 hours ahead
of staining). Making it fresh is important - a stronger stain than
"average" Reynolds, it does not store well. It really helps.

Finally, it helps to include en bloc UA treatment before embedding -
either 2% UA in water before dehydration or 1.5% at the 70% ethanol
step.

This topic has been covered quite a few times on this list. A good
place to search the archives is http://www.biotech.ufl.edu/EM/tips/
You'll find more discussion there.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and not
his employer. These opinions and experiences do not represent a
consensus of the NIH scientific community.
On the good side, this message is not confidential and can be freely
shared and reproduced.



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All,
} I've recently been using TAAB Low Viscosity (TLV) resin
} (since the
} sad demise of Spurr resin!) for embedding standard double-fixed
} cells and
} tissues and sometimes have problems achieving good contrast with
} routine
} uranyl acetate (UA) and Pb citrate post-staining. Has anyone else
} experienced the same problem and found a way to get over it? I guess
} alcoholic UA might be the way to go, but not sure how you rinse the
} grids
} after this.
} Thanks in advance for any advice,
} Julian
}
} Dr. Julian R. Thorpe
} (Office 2C9/Lab 2C11-13)
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
} Tel.: ext +44 (0)1273 877585
} int 7585
}
} URLs:
} (home)
} http://www.sussex.ac.uk/biology/profile2686.html
} (lab)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} (research)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
}
} ==============================Original
} Headers==============================
} 3, 24 -- From bafg3-at-sussex.ac.uk Fri Jan 23 09:57:50 2009
} 3, 24 -- Received: from sivits.uscs.susx.ac.uk
} (sivits.uscs.susx.ac.uk [139.184.14.88])
} 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n0NFvnrr023009
} 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009
} 09:57:49 -0600
} 3, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:
} 2132)
} 3, 24 -- by sivits.uscs.susx.ac.uk with esmtp (Exim 4.64)
} 3, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
} 3, 24 -- id KDXLQ4-000E70-GX
} 3, 24 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009
} 15:58:52 +0000
} 3, 24 -- Date: Fri, 23 Jan 2009 15:57:47 -0000
} 3, 24 -- To: microscopy-at-microscopy.com
} 3, 24 -- Subject: Biological TEM: TAAB Low Viscosity (TLV) resin-
} embedded specimen
} 3, 24 -- section post-staining
} 3, 24 -- Message-ID: {70653603.1232726267-at-ls0130.lifesci.susx.ac.uk}
} 3, 24 -- Originator-Info: login-token=Mulberry:01R
} +B5y6lB3zJ7INklsER1U+4+u0Xk8qg7dVXk;
} 3, 24 -- token_authority=postmaster-at-central.susx.ac.uk
} 3, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
} 3, 24 -- MIME-Version: 1.0
} 3, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
} 3, 24 -- Content-Transfer-Encoding: 7bit
} 3, 24 -- Content-Disposition: inline
} 3, 24 -- X-Sussex: true
} 3, 24 -- X-Sussex-transport: remote_smtp_rew
} 3, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
13, 23 -- From vladislav_speransky-at-nih.gov Fri Jan 23 11:51:55 2009
13, 23 -- Received: from out3.smtp.messagingengine.com (out3.smtp.messagingengine.com [66.111.4.27])
13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NHptkR032232
13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 11:51:55 -0600
13, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
13, 23 -- by out1.fastmail.fm (Postfix) with ESMTP id 1639125590A
13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:51:55 -0500 (EST)
13, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
13, 23 -- by compute2.internal (MEProxy); Fri, 23 Jan 2009 12:51:55 -0500
13, 23 -- X-Sasl-enc: FXVmF70t0dxZCDCFl1RNa5IvlWm6KzyeCgDcCBcn7gbo 1232733114
13, 23 -- Received: from [192.168.0.5] (unknown [96.241.28.40])
13, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id B493720495
13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:51:54 -0500 (EST)
13, 23 -- Message-Id: {0291FDBD-A5DB-44E1-9AF8-7DA14EB03EFC-at-nih.gov}
13, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
13, 23 -- To: Microscopy-at-microscopy.com
13, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 23 -- Content-Transfer-Encoding: 7bit
13, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 23 -- Subject: Fwd: [Microscopy] Biological TEM: TAAB Low Viscosity (TLV) resin-embedded specimen
13, 23 -- Date: Fri, 23 Jan 2009 12:51:53 -0500
13, 23 -- References: {200901231558.n0NFwfqe025306-at-ns.microscopy.com}
13, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: Williams-at-GENECTR.HUNTER.CUNY.EDU
Date: Fri, 23 Jan 2009 12:04:37 -0600
Subject: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am hoping someone on the list can help me
I need to record video from a microscope plus a simultaneous audio commentary, and output both to a AVI/MPG/MOV file. The camera must fit on a microscope C-mount.
I have posted on this before and got recommendations of cameras that produce AVI files via  USB connection, but incorporating live audio so far, is an unresolved problem.
It would seem there is a lot of software that will add a  sound track later but not live.

I can think of two approaches
 1 software: Take a fairly inexpensive camera (Lumenera for instance) interface to a computer, + a standard microphone, and have some software create the AVI  file from both inputs. - So far I haven't found software that will do this at least in real time.

2 Hardware: Take a camera that has built in sound, basically a camcorder, and mount it on a microscope via a C-mount. - I don't know of any camcorders that have c-mount adapters, also I'm not sure if they can be connected to a computer via USB and produce AVI files.

I would rather not go the route of outputting a TV signal to the computer and the adding a digital frame grabbing card to convert the video signal to digital.

Suggestions would be really appreciated

Thanks
Lloyd






Dr. Lloyd Williams
Dept of Biology
Hunter College
695 Park Ave
New York, NY 10021
ph (212) 650 3872
fx (212) 650 3656



==============================Original Headers==============================
14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009
14, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34])
14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NI4Y5A013756
14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34 -0600
14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by
14, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id
14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500
14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by
14, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009
14, 26 -- 13:04:55 -0500
14, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU}
14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
14, 26 -- Date: Fri, 23 Jan 2009 13:04:54 -0500
14, 26 -- Subject: Realtime Audio and Vieo For a C-mount Microscope
14, 26 -- Thread-Topic: Realtime Audio and Vieo For a C-mount Microscope
14, 26 -- Thread-Index: Acl9gio1II/B79NXTe+PQqYZ6YQzbA==
14, 26 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu}
14, 26 -- Accept-Language: en-US
14, 26 -- Content-Language: en-US
14, 26 -- X-MS-Has-Attach:
14, 26 -- X-MS-TNEF-Correlator:
14, 26 -- acceptlanguage: en-US
14, 26 -- Content-Type: text/plain; charset="iso-8859-1"
14, 26 -- MIME-Version: 1.0
14, 26 -- Content-Transfer-Encoding: 8bit
14, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756
==============================End of - Headers==============================




From: joelsheffield-at-gmail.com
Date: Fri, 23 Jan 2009 13:35:52 -0600
Subject: [Microscopy] Re: Realtime Audio and Vieo For a C-mount Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been thinking.....I have a jvc digital movie camera/recorder with a firewire output that I can
connect directly to my computer, and record the results with Windows Media Maker (I think).
This includes both the video and the audio.

As another, inexpensive alternative, what about the "Flip Camera"? It has a usb connector, and
might work. The trick is to interface it with the microscope, but I would imagine that you could
mount it on the eyepiece and adjust focus to match.

Joel





On 23 Jan 2009 at 12:11, Williams-at-GENECTR.HUNTER.CUNY.EDU wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I am hoping someone on the list can help me
} I need to record video from a microscope plus a simultaneous audio commentary, and output both to a AVI/MPG/MOV file. The camera must fit on a microscope C-mount.
} I have posted on this before and got recommendations of cameras that produce AVI files via  USB connection, but incorporating live audio so far, is an unresolved problem.
} It would seem there is a lot of software that will add a  sound track later but not live.
}
} I can think of two approaches
}  1 software: Take a fairly inexpensive camera (Lumenera for instance) interface to a computer, + a standard microphone, and have some software create the AVI  file from both inputs. - So far I haven't found software that will do this at least in real time.
}
} 2 Hardware: Take a camera that has built in sound, basically a camcorder, and mount it on a microscope via a C-mount. - I don't know of any camcorders that have c-mount adapters, also I'm not sure if they can be connected to a computer via USB and produce AVI files.
}
} I would rather not go the route of outputting a TV signal to the computer and the adding a digital frame grabbing card to convert the video signal to digital.
}
} Suggestions would be really appreciated
}
} Thanks
} Lloyd
}
}
}
}
}
}
} Dr. Lloyd Williams
} Dept of Biology
} Hunter College
} 695 Park Ave
} New York, NY 10021
} ph (212) 650 3872
} fx (212) 650 3656
}
}
}
} ==============================Original Headers==============================
} 14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009
} 14, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34])
} 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NI4Y5A013756
} 14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34 -0600
} 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by
} 14, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id
} 14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500
} 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by
} 14, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009
} 14, 26 -- 13:04:55 -0500
} 14, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU}
} 14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 14, 26 -- Date: Fri, 23 Jan 2009 13:04:54 -0500
} 14, 26 -- Subject: Realtime Audio and Vieo For a C-mount Microscope
} 14, 26 -- Thread-Topic: Realtime Audio and Vieo For a C-mount Microscope
} 14, 26 -- Thread-Index: Acl9gio1II/B79NXTe+PQqYZ6YQzbA==
} 14, 26 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu}
} 14, 26 -- Accept-Language: en-US
} 14, 26 -- Content-Language: en-US
} 14, 26 -- X-MS-Has-Attach:
} 14, 26 -- X-MS-TNEF-Correlator:
} 14, 26 -- acceptlanguage: en-US
} 14, 26 -- Content-Type: text/plain; charset="iso-8859-1"
} 14, 26 -- MIME-Version: 1.0
} 14, 26 -- Content-Transfer-Encoding: 8bit
} 14, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756
} ==============================End of - Headers==============================


--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs



==============================Original Headers==============================
13, 43 -- From joelsheffield-at-gmail.com Fri Jan 23 13:35:52 2009
13, 43 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.144])
13, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NJZpjk031195
13, 43 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 13:35:51 -0600
13, 43 -- Received: by qw-out-1920.google.com with SMTP id 4so1045808qwk.54
13, 43 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 11:35:51 -0800 (PST)
13, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 43 -- d=gmail.com; s=gamma;
13, 43 -- h=domainkey-signature:received:received:from:to:date:mime-version
13, 43 -- :subject:message-id:priority:in-reply-to:references:x-mailer
13, 43 -- :content-type:content-transfer-encoding:content-description;
13, 43 -- bh=2n1YesDXumMSS28mxhX9nRmsuMeXJg5Z/y/aM39mq1k=;
13, 43 -- b=hABYOBBq6VdId/oWPsrlu7zYdId9BtgmsHUdAapFB/qWY/wQ+iNLP6XYchbX6WaElP
13, 43 -- DsQB1nA1xr5URxhqcTFZq2w/4nkrZ80komcuOwBPh/HkQlMCNMGkXZnUYqUpuqU1+itE
13, 43 -- l0/gU/o5L2ToHyjD56iejIMZjcvUXbJbkqXo8=
13, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
13, 43 -- d=gmail.com; s=gamma;
13, 43 -- h=from:to:date:mime-version:subject:message-id:priority:in-reply-to
13, 43 -- :references:x-mailer:content-type:content-transfer-encoding
13, 43 -- :content-description;
13, 43 -- b=b3O4txafhkHQFmnlsRh+OyCsdtcJLEHZHNWmRm2T8iwRRtA9eWIQe75jQL8cgPy0eV
13, 43 -- ZV4hEnccIKk+IqEiI0FWbCnS4zcYB/qLmQH3MH88Z/OpBI+UGjSnPQeeUoDloDhyHml9
13, 43 -- RJbKzfjie/LBOJPJXInuN+MR+FfbV6u9vSdDc=
13, 43 -- Received: by 10.229.73.209 with SMTP id r17mr2177478qcj.30.1232739351146;
13, 43 -- Fri, 23 Jan 2009 11:35:51 -0800 (PST)
13, 43 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40])
13, 43 -- by mx.google.com with ESMTPS id 30sm11136473yxk.47.2009.01.23.11.35.49
13, 43 -- (version=SSLv3 cipher=RC4-MD5);
13, 43 -- Fri, 23 Jan 2009 11:35:50 -0800 (PST)
13, 43 -- From: joelsheffield-at-gmail.com
13, 43 -- To: Williams-at-GENECTR.HUNTER.CUNY.EDU, microscopy-at-microscopy.com
13, 43 -- Date: Fri, 23 Jan 2009 14:35:52 -0500
13, 43 -- MIME-Version: 1.0
13, 43 -- Subject: Re: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope
13, 43 -- Message-ID: {4979D5C8.9197.2BA61D9B-at-joelsheffield.gmail.com}
13, 43 -- Priority: normal
13, 43 -- In-reply-to: {200901231811.n0NIB664027188-at-ns.microscopy.com}
13, 43 -- References: {200901231811.n0NIB664027188-at-ns.microscopy.com}
13, 43 -- X-mailer: Pegasus Mail for Windows (4.41)
13, 43 -- Content-type: text/plain; charset=ISO-8859-1
13, 43 -- Content-description: Mail message body
13, 43 -- Content-Transfer-Encoding: 8bit
13, 43 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n0NJZpjk031195
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Sat, 24 Jan 2009 03:01:53 +1100
Subject: [Microscopy] Histo diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------ Forwarded Message
X-from: "Malis, Tom" {Tom.Malis-at-NRCan-RNCan.gc.ca}


Hi Hong,
We have four large histoknives, usually one for trimming, two for everyday
use, and one in perfect shape for when one of the others has to go away for
resharpening. They are used to cut sections up to 2 microns thick, of quite
large blockfaces - up to 3 mm across. They get resharpened at least once a
year. Like Stephane, I'll never go back to routine glass knife use, the
diamond knives save so much time. We only go back to glass for training and
if the tissue might damage the diamond (chunks of rock in soil around roots,
for example...).

cheers,
Roseamry


On 22/01/09 7:47 PM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
{http://www.microscopy.com/MicroscopyListserver}
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
{http://www.microscopy.com/MicroscopyListserver/FAQ.html}
} ----------------------------------------------------------------------
} ------
}
} Hi Hong!
}
} We have a histo knife, however we don't cut thicker than 500nm and not
} on a routine basis.
} I have been said that like an ultraknife, its lifetime mainly depends
} on what you cut. Cut butter and it will survive you.
} Cut nanoparticles and quantum dots and it will probably not survive
} your grant. Cutting soft tissue in resin does probably not significantly
affect it.
} Personally I couldn't imagine regularly semi-thin sectionning without
} histoknife, it is so comfortable. Maybe I am a luxus freak :-)
}
} Regards,
} Stephane
}
}
}
} ----- Original Message ----
} X-from: "hyi-at-emory.edu" {hyi-at-emory.edu}
} To: nizets2-at-yahoo.com
} Sent: Thursday, January 22, 2009 6:34:37 AM
} Subject: [Microscopy] Histo diamond knife
}
}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor:  The Microscopy Society
} of America To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
{http://www.microscopy.com/MicroscopyListserver}
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
{http://www.microscopy.com/MicroscopyListserver/FAQ.html}
} ----------------------------------------------------------------------
} ------
}
} Dear All:
}
}     We have been contemplating about purchasing a 8.0 mm Histo diamond
} knif= e for semi-thin (0.5-0.7=B5m) sectioning.  Can someone out there
} with exper= ience comment on how long (or how many blocks) I should
} expect a Histo diam= ond knife to last?  I have no problem producing
} high quality semi-thin sect= ions with glass knives, but am hoping a
} diamond knife would save us some ti= me.  Thank you in advance.
}
} Hong
} Emory EM
}
}
} This e-mail message (including any attachments) is for the sole use of
} the intended recipient(s) and may contain confidential and privileged
} information.  If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination,
} distribution or copying of this message (including any attachments) is
} strictly prohibited.
}
} If you have received this message in error, please contact the sender
} by reply e-mail message and destroy all copies of the original message
} (including attachments).
}
}
} ==============================Original
} Headers==============================
} 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 7, 32 --
} Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) 7,
} 32 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n0M5U5Rv023498
} 7, 32 --     for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009
} 23:30:05 -0600 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net
} (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 --     by
} mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 7, 32 --    
} for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 7, 32
} -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by 7,
} 32 --  EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft



From: walck-at-southbaytech.com
Date: Fri, 23 Jan 2009 14:16:40 -0600
Subject: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I replied to a similar question last week where I used a Q-see camera that I
hooked up to my video camera. I can then either record it on the video
camera or route it to the computer or a digital recorder. What I didn't say
is that I also hooked up audio with it. The camera that I am using is a
Canon Elura 85. I use the same cord that came with the camera to hook to
the camera and audio with RCA plugs on one end and the three connector
mini-plug that goes into the camera. I set the camera on VCR mode and
there's no problem. I can talk in my gravelly voice and can be heard while
viewing the recorded show. That is a problem.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU
[mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU]
Sent: Friday, January 23, 2009 10:07 AM
To: Walck-at-SouthBayTech.com

I am hoping someone on the list can help me I need to record video from a
microscope plus a simultaneous audio commentary, and output both to a
AVI/MPG/MOV file. The camera must fit on a microscope C-mount.
I have posted on this before and got recommendations of cameras that produce
AVI files via  USB connection, but incorporating live audio so far, is an
unresolved problem.
It would seem there is a lot of software that will add a  sound track later
but not live.

I can think of two approaches
 1 software: Take a fairly inexpensive camera (Lumenera for instance)
interface to a computer, + a standard microphone, and have some software
create the AVI  file from both inputs. - So far I haven't found software
that will do this at least in real time.

2 Hardware: Take a camera that has built in sound, basically a camcorder,
and mount it on a microscope via a C-mount. - I don't know of any camcorders
that have c-mount adapters, also I'm not sure if they can be connected to a
computer via USB and produce AVI files.

I would rather not go the route of outputting a TV signal to the computer
and the adding a digital frame grabbing card to convert the video signal to
digital.

Suggestions would be really appreciated

Thanks
Lloyd






Dr. Lloyd Williams
Dept of Biology
Hunter College
695 Park Ave
New York, NY 10021
ph (212) 650 3872
fx (212) 650 3656



==============================Original Headers==============================
14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009 14,
26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu
[146.95.150.34])
14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0NI4Y5A013756
14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34
-0600
14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 14,
26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server
(TLS) id 14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500 14, 26 --
Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 14, 26 --
Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009
14, 26 -- 13:04:55 -0500 14, 26 -- From: Lloyd Williams
{Williams-at-GENECTR.HUNTER.CUNY.EDU}
14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 26
-- Date: Fri, 23 Jan 2009 13:04:54 -0500 14, 26 -- Subject: Realtime Audio
and Vieo For a C-mount Microscope 14, 26 -- Thread-Topic: Realtime Audio and
Vieo For a C-mount Microscope 14, 26 -- Thread-Index:
Acl9gio1II/B79NXTe+PQqYZ6YQzbA== 14, 26 -- Message-ID:
{A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu}
14, 26 -- Accept-Language: en-US
14, 26 -- Content-Language: en-US
14, 26 -- X-MS-Has-Attach:
14, 26 -- X-MS-TNEF-Correlator:
14, 26 -- acceptlanguage: en-US
14, 26 -- Content-Type: text/plain; charset="iso-8859-1"
14, 26 -- MIME-Version: 1.0
14, 26 -- Content-Transfer-Encoding: 8bit 14, 26 -- X-MIME-Autoconverted:
from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756
==============================End of - Headers==============================

Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
5:29 PM



==============================Original Headers==============================
25, 23 -- From walck-at-southbaytech.com Fri Jan 23 14:16:39 2009
25, 23 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82])
25, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKGb3C027111
25, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:16:38 -0600
25, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201])
25, 23 -- (authenticated bits=0)
25, 23 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0NKGXue004584;
25, 23 -- Fri, 23 Jan 2009 14:16:34 -0600
25, 23 -- From: "Scott Walck" {walck-at-southbaytech.com}
25, 23 -- To: {Microscopy-at-microscopy.com}
25, 23 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU}
25, 23 -- Subject: RE: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope
25, 23 -- Date: Fri, 23 Jan 2009 12:16:50 -0800
25, 23 -- Message-ID: {ADF988ACE1504B72A4AD2742ABEC7803-at-dynamicbl8uno3}
25, 23 -- MIME-Version: 1.0
25, 23 -- Content-Type: text/plain;
25, 23 -- charset="iso-8859-1"
25, 23 -- X-Mailer: Microsoft Office Outlook 11
25, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 23 -- Thread-Index: Acl9hXQtpzSUhkiNSKydCvZDh9oUZAAEP+QA
25, 23 -- In-Reply-To: {200901231807.n0NI7OFa018790-at-ns.microscopy.com}
25, 23 -- Content-Transfer-Encoding: 8bit
25, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NKGb3C027111
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 23 Jan 2009 14:21:31 -0600
Subject: [Microscopy] Nematode permeabilization for fixation and embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have seen some mentions here of methods to enhance fixation and
embedding of nematodes. I just watched my vinegar eels (T. acetii) swim
happily for } 1hr in the [2% glutaraldehyde + 4% paraformaldhyde + 0.2M
cacodylate, pH 7.4] fixative that was reportedly used in some work with
beautiful results - that work reported a 1hr fixation; surely something
is wrong here. I have seen mention of slicing with a razor blade (can it
really be done? How? These vinegar eels are tiny and very wiggly);
penetrating with a pulled micropipette; cryo fixation; enzymatic
digestion of the cuticle (is this done after fixation?); and finally,
laser beams - what type of laser is required - can this be done with a
confocal or does it need a higher power or other wavelengths? I have
some early/mid-1970's papers that have beautiful ultrastructure with no
special methods mentioned at all - maybe not complete disclosure of the
details?

I am wondering if electroporation might be any use to allow quicker
permeabilization of a bulk sample. Does anyone know if electroporation
has ever been used - is this a terrible idea?

I would greatly appreciate any protocols, or references to books or
papers dealing with the nitty-gritty details of such tiny impermeable
preparations.

Thanks!

Dale Callaham

==============================Original Headers==============================
6, 20 -- From dac-at-research.umass.edu Fri Jan 23 14:21:30 2009
6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKLUjK004106
6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:21:30 -0600
6, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
6, 20 -- (authenticated bits=0)
6, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0NKLT93017629
6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 15:21:30 -0500
6, 20 -- Message-ID: {497A2712.6060305-at-research.umass.edu}
6, 20 -- Date: Fri, 23 Jan 2009 15:22:42 -0500
6, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
6, 20 -- Reply-To: dac-at-research.umass.edu
6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
6, 20 -- MIME-Version: 1.0
6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 20 -- Subject: Nematode permeabilization for fixation and embedding
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 23 Jan 2009 14:51:11 -0600
Subject: [Microscopy] Searching for beryllium in all the wrong places.....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been working with a polished copper bar and found some inclusions.
EDS shows nothing but copper and of course it can't detect beryllium. It
occurred to me that beryllium has such a low backscatter coefficient as
compared to copper and areas enriched in berllium should appear dark in
compo mode.

Would it be reasonable to say that any dark areas in BS imaging what show
only copper could have beryllium enrichment? And while I'm on the subject,
does any one have a copy of the condensed micro-chemical test for beryllium
that Dr. McCone published years ago in the Microscope? If so could I get a
copy?

Thanks,
Frank Karl
Lincoln Electric

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009
6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKpA31022903
6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:51:10 -0600
6, 21 -- Subject: Searching for beryllium in all the wrong places.....
6, 21 -- To: Microscopy-at-microscopy.com
6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
6, 21 -- Message-ID: {OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectric.com}
6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500
6, 21 -- From: Frank_Karl-at-lincolnelectric.com
6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM,
6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: text/plain;
6, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 23 Jan 2009 14:56:05 -0600
Subject: [Microscopy] RE: viaWWW: TEM of TiO2 nanotubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suggest using alcohol (better adhesion) for deposition of
nanotubes on carbon film (only carbon, to decrease charging). Usually
you can find a lot of nanotubes poking out of edges of clamps, so good
dispersion of specimens in not a mandatory thing. You can also try to
deposit (with alcohol) clamps of nanotubes directly on Cu grid without
any film. Sometimes it can give better results (if you can find these
clamps tracing outline of grid) - contrast and resolution are better
without film.

Good luck,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Title-Subject: [Filtered] TEM of TiO2 nanotubes
}
} Question: Hi all
}
} I have been working with a researcher here looking at some
} TiO2 Nanotubes in the TEM. The researcher wants to see if
} his nanotubes are hollow or not. We are looking for a core
} of around 5 nm.
}
} Being a biology lab we do not have any experience with such samples.
} I have tried dusting the fragments (provided in powder form
} from scrappings off a Ti plate) onto carbon/formvar grids
} and I have tried drying them onto a grid from a suspension in
} ethanol. The solvent method proved more successful at
} getting particles onto the film than the dusting method
} however in both cases they tend to be clumped.
}
} The problem I have is the clumps must be heating up in the
} beam. I as soon as I start to increase the magnification to
} explore the edges the sample disappears and I am left with a
} hole in the film.
}
} I am pushing a 100kV instrument so I suspect this may have
} something to with it also.
}
} A search of the literature indicates that a lot of people are
} investigating TiO2 nanotubes in the TEM but nothing I have
} found so far talks about how the nanotubes are got onto a
} grid in a usable form to image. lots of info about making nanotubes.
}
} Any thoughts or suggestions would be appreciated.
}
} Regards
}
} Allan
}
}
} Allan Mitchell
} Otago Centre for Electron Microscopy
} Department of Anatomy and Structural Biology School of
} Medical Sciences P.O. Box 913 Dunedin New Zealand
}
} Phone (03) 479 5642 or 479 7301
} Fax (03) 479 5086 or 479 7254
}
} EM Centre: http://ocem.otago.ac.nz/
} Confocal Centre: http://occm.otago.ac.nz/
} Department: http://anatomy.otago.ac.nz/
}
} Login Host: 139.80.40.92
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 18, 11 -- From zaluzec-at-microscopy.com Thu Jan 22 08:11:29
} 2009 18, 11 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
} 18, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0MEBTKc026853
} 18, 11 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan
} 2009 08:11:29 -0600
} 18, 11 -- Mime-Version: 1.0
} 18, 11 -- Message-Id: {p06240801c59e2edb6749-at-[206.69.208.22]}
} 18, 11 -- Date: Thu, 22 Jan 2009 08:11:28 -0600 18, 11 -- To:
} microscopy-at-microscopy.com 18, 11 -- From:
} allan.mitchell-at-stonebow.otago.ac.nz (by way of
} MicroscopyListserver) 18, 11 -- Subject: viaWWW: TEM of TiO2
} nanotubes 18, 11 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Fri Jan 23 14:56:05 2009
8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp1.exchange.umkc.edu [134.193.143.155])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKu4SQ031946
8, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:56:05 -0600
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Fri, 23 Jan 2009 14:56:04 -0600
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="us-ascii"
8, 25 -- Subject: RE: [Microscopy] viaWWW: TEM of TiO2 nanotubes
8, 25 -- Date: Fri, 23 Jan 2009 14:56:03 -0600
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BD-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200901221411.n0MEBvx8027718-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] viaWWW: TEM of TiO2 nanotubes
8, 25 -- thread-index: Acl8m2YmfonpwTiFSwivxJg30W7fNQA/8LLg
8, 25 -- References: {200901221411.n0MEBvx8027718-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {allan.mitchell-at-stonebow.otago.ac.nz} , {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 23 Jan 2009 20:56:04.0271 (UTC) FILETIME=[0158E3F0:01C97D9D]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NKu4SQ031946
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Fri, 23 Jan 2009 14:58:00 -0600
Subject: [Microscopy] Re: Nematode permeabilization for fixation and embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dale-
I've never worked with your beasties, but have been doing both EM and
confocal on sea squirt (Ciona intestinalis) larvae with one of the
PIs here. She uses electroporation to get her GFP constructs into
the very young larvae then lets them mature to the stage she is
interested in studying...so that method along does not kill them
(usually), but does "open them up" to things.
For the EM studies, we used 2.5% glut, 4% pfa in buffer at 4 degrees
overnight, then proceeded pretty much as normal, except that we did 2
changes at each alcohol level and prolonged the infiltration (over 2
days). We used Spurr's at the time.
The C. elegans people love high pressure freezing followed by freeze
substitution. When it works, it is gorgeous.
Lee








} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
12, 25 -- From lcgould-at-med.cornell.edu Fri Jan 23 14:58:00 2009
12, 25 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKvxE4005719
12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:58:00 -0600
12, 25 -- Received: from mpx3.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120])
12, 25 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0NKvnIv026213;
12, 25 -- Fri, 23 Jan 2009 15:57:58 -0500 (EST)
12, 25 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
12, 25 -- by mpx3.med.cornell.edu
12, 25 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
12, 25 -- with ESMTPA id {0KDX001MEZKIKQ40-at-mpx3.med.cornell.edu} ; Fri,
12, 25 -- 23 Jan 2009 15:57:55 -0500 (EST)
12, 25 -- Date: Fri, 23 Jan 2009 15:57:52 -0500
12, 25 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
12, 25 -- Subject: Re: [Microscopy] Nematode permeabilization for fixation and embedding
12, 25 -- In-reply-to: {200901232027.n0NKRjPE020899-at-ns.microscopy.com}
12, 25 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
12, 25 -- To: dac-at-research.umass.edu, Microscopy Listserver {microscopy-at-microscopy.com}
12, 25 -- Message-id: {p0623090ac59fde44e0ea-at-[140.251.48.23]}
12, 25 -- MIME-version: 1.0
12, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed
12, 25 -- Content-transfer-encoding: 7BIT
12, 25 -- References: {200901232027.n0NKRjPE020899-at-ns.microscopy.com}
12, 25 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.1.23.204648
12, 25 -- X-PerlMx-Spam: Gauge=, IIIIIII, Probability=7%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P2 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0'
==============================End of - Headers==============================




From: Joseph_Oparowski-at-bose.com
Date: Fri, 23 Jan 2009 15:13:12 -0600
Subject: [Microscopy] Searching for beryllium in all the wrong places.....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,

Yes, beryllium rich phases should exhibit lower atomic number contrast via BSe. If you have a beryllium-copper alloy that has been precipitation hardened, the CuBe phase should be dispersed evenly throughout the matrix as numerous spherical precipitates. They should also appear gray in optical brightfield and full wave polarized light if I recall.

If you are seeing isolated inclusions, they may be oxides, which will appear ruby red in full wave polarized light.

Care to provide a link to an image?


Joseph M. Oparowski
Research Engineer
Transducer Research
The Mountain M/S 470
Framingham, MA 01701-9168

Phone: (508) 766-1371
Fax: (508) 518-6515
email: joseph_oparowski-at-bose.com

-----Original Message-----
X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com]
Sent: Friday, January 23, 2009 3:59 PM
To: Oparowski, Joseph


I've been working with a polished copper bar and found some inclusions.
EDS shows nothing but copper and of course it can't detect beryllium. It
occurred to me that beryllium has such a low backscatter coefficient as
compared to copper and areas enriched in berllium should appear dark in
compo mode.

Would it be reasonable to say that any dark areas in BS imaging what show
only copper could have beryllium enrichment? And while I'm on the subject,
does any one have a copy of the condensed micro-chemical test for beryllium
that Dr. McCone published years ago in the Microscope? If so could I get a
copy?

Thanks,
Frank Karl
Lincoln Electric

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009
6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKpA31022903
6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:51:10 -0600
6, 21 -- Subject: Searching for beryllium in all the wrong places.....
6, 21 -- To: Microscopy-at-microscopy.com
6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
6, 21 -- Message-ID: {OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectric.com}
6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500
6, 21 -- From: Frank_Karl-at-lincolnelectric.com
6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM,
6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: text/plain;
6, 21 -- charset="US-ASCII"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 26 -- From Joseph_Oparowski-at-bose.com Fri Jan 23 15:13:12 2009
20, 26 -- Received: from usmamx02.bose.com (usmamx02.bose.com [139.68.146.59])
20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NLDBYE031580
20, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 23 Jan 2009 15:13:11 -0600
20, 26 -- X-PMWin-Version: 3.0.1.0, Antivirus-Engine: 2.80.0, Antivirus-Data: 4.35E
20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
20, 26 -- Received: from usmaexht02.bose.com ([10.100.10.8]) by usmamx02.bose.com with Microsoft SMTPSVC(6.0.3790.3959); Fri, 23 Jan 2009 16:13:11 -0500
20, 26 -- Received: from usmaexmb01.bose.com ([10.101.20.21]) by usmaexht02.bose.com ([10.102.20.24]) with mapi; Fri, 23 Jan 2009 16:13:11 -0500
20, 26 -- From: "Oparowski, Joseph" {Joseph_Oparowski-at-bose.com}
20, 26 -- To: {Microscopy-at-Microscopy.Com}
20, 26 -- Date: Fri, 23 Jan 2009 16:13:09 -0500
20, 26 -- Subject: FW: [Microscopy] Searching for beryllium in all the wrong places.....
20, 26 -- Thread-Topic: [Microscopy] Searching for beryllium in all the wrong places.....
20, 26 -- thread-index: Acl9nX62f6VSMWucTuS9daFPGU57owAAIKxQ
20, 26 -- Message-ID: {85D771575E0D11479A44687ED1A2F6006372E8-at-usmaexmb01.bose.com}
20, 26 -- Accept-Language: en-US
20, 26 -- Content-Language: en-US
20, 26 -- X-MS-Has-Attach:
20, 26 -- X-MS-TNEF-Correlator:
20, 26 -- acceptlanguage: en-US
20, 26 -- Content-Type: text/plain;
20, 26 -- charset="us-ascii"
20, 26 -- MIME-Version: 1.0
20, 26 -- X-OriginalArrivalTime: 23 Jan 2009 21:13:11.0545 (UTC) FILETIME=[65A67690:01C97D9F]
20, 26 -- Content-Transfer-Encoding: 8bit
20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NLDBYE031580
==============================End of - Headers==============================




From: p.ingram-at-voice.cellbio.duke.edu
Date: Fri, 23 Jan 2009 15:54:32 -0600
Subject: [Microscopy] Re: Searching for beryllium in all the wrong

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,

Most assuredly it is possible to detect Be with EDS - which
obviously must have a UTW detector. Although we were using biological
materials that are mostly carbon, it is absolutely no problem with
higher Z materials such as Al. I haven't tried Cu but as long as
there are no other overlapping peaks around 0.11 Kev it should not be
a problem. One caveat: you do have to be careful to tweak the
"threshold" for the pulse processor to minimize the potential
detector "noise" that can creep in! Here is a reference of ours from
a few years ago:

Butnor KJ, Sporn TA, Ingram P, Gunasegaram S, Pinto JF, Roggli VL.
Beryllium detection in human lung tissue using electron probe X-ray
microanalysis, Mod Pathol. 2003 Nov;16(11):1171-7

Feel free to contact me off-line if you would like to discuss further.

Peter




}
}
} I've been working with a polished copper bar and found some inclusions.
} EDS shows nothing but copper and of course it can't detect beryllium. It
} occurred to me that beryllium has such a low backscatter coefficient as
} compared to copper and areas enriched in berllium should appear dark in
} compo mode.
}
} Would it be reasonable to say that any dark areas in BS imaging what show
} only copper could have beryllium enrichment? And while I'm on the subject,
} does any one have a copy of the condensed micro-chemical test for beryllium
} that Dr. McCone published years ago in the Microscope? If so could I get a
} copy?
}
} Thanks,
} Frank Karl
} Lincoln Electric
}
}


--
Peter Ingram
Sr. Physicist
Adj. Professor of Pathology,
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu
http://152.3.54.111/AEM_LAB.html

==============================Original Headers==============================
12, 22 -- From p.ingram-at-voice.cellbio.duke.edu Fri Jan 23 15:54:32 2009
12, 22 -- Received: from smtp.duke.edu (smtp-02.oit.duke.edu [152.3.174.84])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NLsWhe014291
12, 22 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 15:54:32 -0600
12, 22 -- Received: from smtp.duke.edu (localhost.localdomain [127.0.0.1])
12, 22 -- by localhost (Postfix) with SMTP id 31E72510BDE;
12, 22 -- Fri, 23 Jan 2009 16:54:32 -0500 (EST)
12, 22 -- Received: from [152.3.54.111] (dhcp111.fel.duke.edu [152.3.54.111])
12, 22 -- by smtp.duke.edu (Postfix) with ESMTP id 7A71C510B9E;
12, 22 -- Fri, 23 Jan 2009 16:54:31 -0500 (EST)
12, 22 -- Mime-Version: 1.0
12, 22 -- Message-Id: {a05200f00c59fea45d738-at-[152.3.54.111]}
12, 22 -- In-Reply-To: {200901232053.n0NKrbRH027333-at-ns.microscopy.com}
12, 22 -- References: {200901232053.n0NKrbRH027333-at-ns.microscopy.com}
12, 22 -- Date: Fri, 23 Jan 2009 16:54:29 -0500
12, 22 -- To: Frank_Karl-at-lincolnelectric.com
12, 22 -- From: Peter Ingram {p.ingram-at-voice.cellbio.duke.edu}
12, 22 -- Subject: Re: [Microscopy] Searching for beryllium in all the wrong
12, 22 -- places.....
12, 22 -- Cc: microscopy-at-microscopy.com
12, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
12, 22 -- X-PMX-Version: 5.4.2.338381, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.1.23.213726
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Fri, 23 Jan 2009 15:59:43 -0600
Subject: [Microscopy] Searching for beryllium in all the wrong

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank;
It would be a big surprise to me to see beryllium in a bar, as
it is normally used to precipitation harden copper for springs. Also,
the precipitates would be expected to be very small and well dispersed,
not in inclusions.

John Mardinly,
Numonyx

-----Original Message-----
X-from: Frank_Karl-at-lincolnelectric.com
[mailto:Frank_Karl-at-lincolnelectric.com]
Sent: Friday, January 23, 2009 12:58 PM
To: MARDINLY, A


I've been working with a polished copper bar and found some inclusions.
EDS shows nothing but copper and of course it can't detect beryllium.
It
occurred to me that beryllium has such a low backscatter coefficient as
compared to copper and areas enriched in berllium should appear dark in
compo mode.

Would it be reasonable to say that any dark areas in BS imaging what
show
only copper could have beryllium enrichment? And while I'm on the
subject,
does any one have a copy of the condensed micro-chemical test for
beryllium
that Dr. McCone published years ago in the Microscope? If so could I
get a
copy?

Thanks,
Frank Karl
Lincoln Electric

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original
Headers==============================
6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009
6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com
[64.109.211.115])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0NKpA31022903
6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009
14:51:10 -0600
6, 21 -- Subject: Searching for beryllium in all the wrong places.....
6, 21 -- To: Microscopy-at-microscopy.com
6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
6, 21 -- Message-ID:
{OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectr
ic.com}
6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500
6, 21 -- From: Frank_Karl-at-lincolnelectric.com
6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM,
6, 21 -- Itemize by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM,
6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: text/plain;
6, 21 -- charset="US-ASCII"
==============================End of -
Headers==============================



==============================Original Headers==============================
14, 29 -- From A.MARDINLY-at-numonyx.com Fri Jan 23 15:59:43 2009
14, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38])
14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NLxgN0024318
14, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 23 Jan 2009 15:59:43 -0600
14, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23])
14, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 0300C414460;
14, 29 -- Fri, 23 Jan 2009 16:26:52 -0500 (EST)
14, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
14, 29 -- Fri, 23 Jan 2009 16:59:41 -0500
14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 29 -- Content-class: urn:content-classes:message
14, 29 -- MIME-Version: 1.0
14, 29 -- Content-Type: text/plain;
14, 29 -- charset="us-ascii"
14, 29 -- Subject: RE: [Microscopy] Searching for beryllium in all the wrong places.....
14, 29 -- Date: Fri, 23 Jan 2009 16:59:40 -0500
14, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9D60A71-at-EXDRESBENMX012.numonyx.local}
14, 29 -- In-Reply-To: {200901232057.n0NKvqmA005293-at-ns.microscopy.com}
14, 29 -- X-MS-Has-Attach:
14, 29 -- X-MS-TNEF-Correlator:
14, 29 -- Thread-Topic: [Microscopy] Searching for beryllium in all the wrong places.....
14, 29 -- Thread-Index: Acl9nVd8ZnX5e4STQCiwd7fzN8KeHAACBpBA
14, 29 -- References: {200901232057.n0NKvqmA005293-at-ns.microscopy.com}
14, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
14, 29 -- To: {Frank_Karl-at-lincolnelectric.com}
14, 29 -- Cc: {Microscopy-at-Microscopy.com}
14, 29 -- X-OriginalArrivalTime: 23 Jan 2009 21:59:41.0752 (UTC) FILETIME=[E4BE4380:01C97DA5]
14, 29 -- Content-Transfer-Encoding: 8bit
14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NLxgN0024318
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 23 Jan 2009 17:17:47 -0600
Subject: [Microscopy] viaWWW: TAAB Low Viscosity (TLV) Resin Post-Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.r.thorpe-at-sussex.ac.uk as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.r.thorpe-at-sussex.ac.uk
Name: Julian Thorpe

Organization: University of Sussex

Title-Subject: [Filtered] TAAB Low Viscosity (TLV) Resin Post-Staining

Question: Dear All,
I've recently been using TAAB Low Viscosity (TLV) resin (since
Spurr resin became unavailable, sadly!). Anyway, I've had some
problems with some specimens in achieving good contrast with routine
uranyl acetate (UA) and Pb citrate post-staining. Has anyone else
found this to be the case? If so, and you've managed to get around
this problem, I'd be happy to hear from you. I guess alcoholic UA
might be the way to go, but I've often had problems rinsing this off
properly.
Thanks for any advice in advance,
Julian

Dr. Julian R. Thorpe
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

Login Host: 139.184.30.134
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Jan 23 17:17:47 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NNHkkQ011266
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 17:17:46 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c5a000888df6-at-[206.69.208.22]}
8, 11 -- Date: Fri, 23 Jan 2009 17:17:44 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: TAAB Low Viscosity (TLV) Resin Post-Staining
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kmaclellan-at-nibsc.ac.uk
Date: Fri, 23 Jan 2009 17:18:25 -0600
Subject: [Microscopy] viaWWW: First announcement: Workshop on Structural &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kmaclellan-at-nibsc.ac.uk as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kmaclellan-at-nibsc.ac.uk
Name: Kirsty MacLellan

Organization: nibsc

Title-Subject: [Filtered] First announcement:
Workshop on Structural & Computational Biomedical
Informatics and Cryo-EM

Question: Professor Wah Chiu Baylor College of
Medicine, JEOL (UK) Ltd and The National
Institute for Biological Standards and Control
are holding the Workshop on Structural &
Computational Biomedical Informatics and Cryo-EM.
The workshop is a 5 day residential course on
June 8th ñJune 12th 2009. Topics to be covered
include: Specimen preparation, electron optics
involved, configuration of the TEM and
understanding specimen beam interaction, Data
size ñ Assessment and Data Processing, Automated
Data Collection for Single Particle, Auto-data
collection for Tomography, JADAS and EMAN 2 with
hands on practical experience in the microscopy
suite and the computer lab.

Further details and registration forms can be
found at
http://ncmi.bcm.edu/ncmi/events/workshops/workshops_97.
Early registration is essential as places are
limited.


Login Host: 212.219.216.54
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 14 -- From zaluzec-at-microscopy.com Fri Jan 23 17:18:25 2009
9, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NNIOKv011854
9, 14 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 17:18:25 -0600
9, 14 -- Mime-Version: 1.0
9, 14 -- Message-Id: {p06240801c5a0009f9360-at-[206.69.208.22]}
9, 14 -- Date: Fri, 23 Jan 2009 17:18:23 -0600
9, 14 -- To: microscopy-at-microscopy.com
9, 14 -- From: kmaclellan-at-nibsc.ac.uk (by way of MicroscopyListserver)
9, 14 -- Subject: viaWWW: First announcement: Workshop on Structural &
9, 14 -- Computational Biomedical Informatics and Cryo-EM
9, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 14 -- Content-Transfer-Encoding: 8bit
9, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NNIOKv011854
==============================End of - Headers==============================




From: chyun-at-uark.edu
Date: Fri, 23 Jan 2009 17:19:03 -0600
Subject: [Microscopy] viaWWW: Convert EELS data from text file format to 'Gatan DM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both chyun-at-uark.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: chyun-at-uark.edu
Name: Changbae Hyun

Organization: University of Arkansas

Title-Subject: [Filtered] Convert EELS data from
text file format to 'Gatan DM imageDocument3í

Question:
Is there a way to convert a EELS data from text
file to Gatan DM imageDocument3 file format? I
have electron counts vs. energy and I want to use
Gatan's Digital Micrograph program to analyze
EELS data. If anyone knows the way to convert,
please let me know.

Thanks,
Changbae Hyun
PostDoc fellow
University of Arkansas
800 W Dickson St, RM226
Fayetteville, AR 72701
e-mail:chyun-at-uark.edu
tel:479-575-8764

Login Host: 130.184.202.76
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 14 -- From zaluzec-at-microscopy.com Fri Jan 23 17:19:02 2009
8, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NNJ2VW013842
8, 14 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 17:19:02 -0600
8, 14 -- Mime-Version: 1.0
8, 14 -- Message-Id: {p06240802c5a000c69ca0-at-[206.69.208.22]}
8, 14 -- Date: Fri, 23 Jan 2009 17:19:00 -0600
8, 14 -- To: microscopy-at-microscopy.com
8, 14 -- From: chyun-at-uark.edu (by way of MicroscopyListserver)
8, 14 -- Subject: viaWWW: Convert EELS data from text file format to 'Gatan DM
8, 14 -- =?iso-8859-1?Q?imageDocument3=ED?=
8, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 14 -- Content-Transfer-Encoding: 8bit
8, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NNJ2VW013842
==============================End of - Headers==============================




From: rao.karavadi-at-rutgers.edu
Date: Fri, 23 Jan 2009 17:19:35 -0600
Subject: [Microscopy] viaWWW: Multiple EELS acquisition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rao.karavadi-at-rutgers.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rao.karavadi-at-rutgers.edu
Name: Rao Karavadi

Organization: Rutgers University

Title-Subject: [Filtered] Multiple EELS acquisition

Question: Hi all

I am looking for a Digital Micrograph script file, where we can
acquire Multiple EELS spectrum with regular intervals of time. I am
interested to study radiation damage by Electron beam on different
materials which requires collections of EELS spectra with regular
intervals of time.

I truly appreciate your response

Thanks
Rao Karavadi
Rutgers: The State University of New Jersey
607 Taylor Road
Piscataway, NJ 08854
USA


Login Host: 128.6.64.150
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Fri Jan 23 17:19:35 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NNJY7e015671
10, 11 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 17:19:34 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c5a000f2a6dd-at-[206.69.208.22]}
10, 11 -- Date: Fri, 23 Jan 2009 17:19:33 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: rao.karavadi-at-rutgers.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Multiple EELS acquisition
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Fri, 23 Jan 2009 18:10:38 -0600
Subject: [Microscopy] Re: Nematode permeabilization for fixation and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dale,
The standard method for fixing drosophila embryos which have an
impermeable cuticle is to use fix with heptane, a partition method.
Briefly, put 5ml of your fix and 5ml of heptane in a 20ml vial. Add your
sample, screw the cap on very well and agitate vigorously for 20--30
minutes. As the embryos fix they swell and usually break the cuticle.
The cuticle free embryos will sink to the bottom in the fix once you
stop shaking the vial. Those fixed embryos can be pipetted into another
clean vial and fixed longer or rinsed in buffer followed by the standard
EM preparation protocols. If this doesn't work--it doesn't work for
drosophila larvae, then cool the samples on ice until they stop wiggling
and then slice off one end with a razor blade. I like to use a piece of
dental wax or polyethylene plastic for the slicing surface. The scoop
the samples into fix usually for overnight.
Best wishes
Larry

dac-at-research.umass.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi all,
}
} I have seen some mentions here of methods to enhance fixation and
} embedding of nematodes. I just watched my vinegar eels (T. acetii) swim
} happily for } 1hr in the [2% glutaraldehyde + 4% paraformaldhyde + 0.2M
} cacodylate, pH 7.4] fixative that was reportedly used in some work with
} beautiful results - that work reported a 1hr fixation; surely something
} is wrong here. I have seen mention of slicing with a razor blade (can it
} really be done? How? These vinegar eels are tiny and very wiggly);
} penetrating with a pulled micropipette; cryo fixation; enzymatic
} digestion of the cuticle (is this done after fixation?); and finally,
} laser beams - what type of laser is required - can this be done with a
} confocal or does it need a higher power or other wavelengths? I have
} some early/mid-1970's papers that have beautiful ultrastructure with no
} special methods mentioned at all - maybe not complete disclosure of the
} details?
}
} I am wondering if electroporation might be any use to allow quicker
} permeabilization of a bulk sample. Does anyone know if electroporation
} has ever been used - is this a terrible idea?
}
} I would greatly appreciate any protocols, or references to books or
} papers dealing with the nitty-gritty details of such tiny impermeable
} preparations.
}
} Thanks!
}
} Dale Callaham
}
} ==============================Original Headers==============================
} 6, 20 -- From dac-at-research.umass.edu Fri Jan 23 14:21:30 2009
} 6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
} 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKLUjK004106
} 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:21:30 -0600
} 6, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
} 6, 20 -- (authenticated bits=0)
} 6, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0NKLT93017629
} 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 15:21:30 -0500
} 6, 20 -- Message-ID: {497A2712.6060305-at-research.umass.edu}
} 6, 20 -- Date: Fri, 23 Jan 2009 15:22:42 -0500
} 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
} 6, 20 -- Reply-To: dac-at-research.umass.edu
} 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
} 6, 20 -- MIME-Version: 1.0
} 6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} 6, 20 -- Subject: Nematode permeabilization for fixation and embedding
} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 6, 20 -- Content-Transfer-Encoding: 7bit
} 6, 20 -- X-Whitelist: TRUE
} ==============================End of - Headers==============================
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
6, 39 -- From Larry.Ackerman-at-ucsf.edu Fri Jan 23 18:10:37 2009
6, 39 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98])
6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0O0Aaw9001773
6, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 18:10:37 -0600
6, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with
6, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
6, 39 -- Fri, 23 Jan 2009 16:10:25 -0800
6, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
6, 39 -- X-AuditID: 403623d2-ac61fbb000007fb9-63-497a78ab53a6
6, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org (
6, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by
6, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
6, 39 -- 4F016129D; Fri, 23 Jan 2009 18:10:51 -0800 (PST)
6, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
6, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830);
6, 39 -- Fri, 23 Jan 2009 16:10:24 -0800
6, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
6, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Fri, 23 Jan
6, 39 -- 2009 16:10:24 -0800
6, 39 -- Message-ID: {497A5C6F.5010206-at-ucsf.edu}
6, 39 -- Date: Fri, 23 Jan 2009 16:10:23 -0800
6, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
6, 39 -- Reply-to: larry.ackerman-at-ucsf.edu
6, 39 -- Organization: UCSF, NeuroAnatomy
6, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 39 -- MIME-Version: 1.0
6, 39 -- To: dac-at-research.umass.edu, Microscopy-at-microscopy.com
6, 39 -- Subject: Re: [Microscopy] Nematode permeabilization for fixation and
6, 39 -- embedding
6, 39 -- References: {200901232026.n0NKQfjr018546-at-ns.microscopy.com}
6, 39 -- In-Reply-To: {200901232026.n0NKQfjr018546-at-ns.microscopy.com}
6, 39 -- X-OriginalArrivalTime: 24 Jan 2009 00:10:24.0214 (UTC)
6, 39 -- FILETIME=[2736FF60:01C97DB8]
6, 39 -- X-Brightmail-Tracker: AAAAAQ1Okks=
6, 39 -- X-WSS-ID: 656483FB1SS902737-01-01
6, 39 -- Content-Type: text/plain;
6, 39 -- charset=iso-8859-1;
6, 39 -- format=flowed
6, 39 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Aleksandr.Mironov-at-manchester.ac.uk
Date: Sat, 24 Jan 2009 06:57:08 -0600
Subject: [Microscopy] Re: TAAB Low Viscosity (TLV) Resin Post-Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are using TAAB LV for several years since Spurr's became
unavailble. We had never any problems with staining or infiltraton if
propylen oxide is used as intermediate. Only once 2-3 years ago there
was a batch that was not possible to cut with glass knives but only
with diamond ones. Just another happy customer - no any commercial
interest.
Both aqueous and alcohol solution of UA work well. Reynolds Pb citrate
gives very good contrast.

Sincerely,
Alex

--
Dr. Aleksandr Mironov MD, PhD
Experimental Officer
D.1527, M.Smith Building
EM Core Facility, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-5645
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
http://www.ls.manchester.ac.uk/research/facilities/electronmicroscopy/


==============================Original Headers==============================
5, 36 -- From Aleksandr.Mironov-at-manchester.ac.uk Sat Jan 24 06:57:08 2009
5, 36 -- Received: from serenity.mcc.ac.uk (serenity.mcc.ac.uk [130.88.200.93])
5, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0OCv7T7018355
5, 36 -- for {microscopy-at-microscopy.com} ; Sat, 24 Jan 2009 06:57:08 -0600
5, 36 -- Received: from kelvin.its.manchester.ac.uk ([130.88.25.195])
5, 36 -- by serenity.mcc.ac.uk with esmtps (TLSv1:AES256-SHA:256)
5, 36 -- (Exim 4.69 (FreeBSD))
5, 36 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} )
5, 36 -- id 1LQi4Q-0007Vl-Or
5, 36 -- for microscopy-at-microscopy.com; Sat, 24 Jan 2009 12:57:06 +0000
5, 36 -- Received: from webmail-w4.its.manchester.ac.uk ([10.2.61.7]:41312 helo=webmail.manchester.ac.uk)
5, 36 -- by kelvin.its.manchester.ac.uk with esmtpa (Exim 4.69)
5, 36 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} )
5, 36 -- id 1LQi4Q-0005z3-LX
5, 36 -- for microscopy-at-microscopy.com; Sat, 24 Jan 2009 12:57:06 +0000
5, 36 -- Received: from cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com
5, 36 -- (cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com [80.2.178.23]) by
5, 36 -- webmail.manchester.ac.uk (Horde Framework) with HTTP; Sat, 24 Jan 2009
5, 36 -- 12:57:06 +0000
5, 36 -- Message-ID: {20090124125706.13054u04revo1gys-at-webmail.manchester.ac.uk}
5, 36 -- X-Priority: 3 (Normal)
5, 36 -- Date: Sat, 24 Jan 2009 12:57:06 +0000
5, 36 -- From: "Aleksandr Mironov" {Aleksandr.Mironov-at-manchester.ac.uk}
5, 36 -- To: microscopy-at-microscopy.com
5, 36 -- Subject: [Microscopy] Re: TAAB Low Viscosity (TLV) Resin Post-Staining
5, 36 -- MIME-Version: 1.0
5, 36 -- Content-Type: text/plain;
5, 36 -- charset=windows-1251;
5, 36 -- DelSp="Yes";
5, 36 -- format="flowed"
5, 36 -- Content-Disposition: inline
5, 36 -- Content-Transfer-Encoding: 7bit
5, 36 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.6)
5, 36 -- X-Authenticated-Sender: Aleksandr Mironov from webmail-w4.its.manchester.ac.uk (webmail.manchester.ac.uk) [10.2.61.7]:41312
5, 36 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk
5, 36 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details.
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Mon, 26 Jan 2009 12:24:16 -0600
Subject: [Microscopy] RE: viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:

Thank you all very much for so many advices on Histo diamond knife. I
must have become one of those people I used to laugh at who live on the old
time glory. ;-)

Hong
Emory EM
(404) 712-8491


This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).


==============================Original Headers==============================
7, 32 -- From hyi-at-emory.edu Sat Jan 24 08:38:38 2009
7, 32 -- Received: from mr4.cc.emory.edu (mr4.cc.emory.edu [170.140.52.93])
7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0OEccPY011535
7, 32 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Jan 2009 08:38:38 -0600
7, 32 -- Received: from EXCHEDGE4.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
7, 32 -- by mr4.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0OEcWTJ029951
7, 32 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Jan 2009 09:38:32 -0500
7, 32 -- Received: from EXCHHUB2.Enterprise.emory.net (170.140.30.54) by
7, 32 -- EXCHEDGE4.enterprise.emory.net (170.140.52.36) with Microsoft SMTP Server
7, 32 -- (TLS) id 8.1.336.0; Sat, 24 Jan 2009 09:38:26 -0500
7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
7, 32 -- EXCHHUB2.Enterprise.emory.net ([170.140.30.54]) with mapi; Sat, 24 Jan 2009
7, 32 -- 09:38:32 -0500
7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 32 -- Date: Sat, 24 Jan 2009 09:38:31 -0500
7, 32 -- Subject: Thank you
7, 32 -- Thread-Topic: Thank you
7, 32 -- Thread-Index: Acl+MW1btmTdhjLgDU6c5b5e6ffXtg==
7, 32 -- Message-ID: {C5A09217.14A8%hyi-at-emory.edu}
7, 32 -- Accept-Language: en-US
7, 32 -- Content-Language: en
7, 32 -- X-MS-Has-Attach:
7, 32 -- X-MS-TNEF-Correlator:
7, 32 -- acceptlanguage: en-US
7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
7, 32 -- MIME-Version: 1.0
7, 32 -- X-emory.edu-MailScanner: Found to be clean
7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
7, 32 -- X-Spam-Status: No
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0OEccPY011535
==============================End of - Headers==============================

From swoge-at-gmx.de Sat Jan 24 11:25:16 2009
Return-Path: {swoge-at-gmx.de}
Received: from google.com (86-63-99-118.sta.asta-net.com.pl [86.63.99.118])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0OHPFDS005839
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 24 Jan 2009 11:25:15 -0600
Received: from [110.143.58.79] (HELO google.com)
by kind-hoosegow.net; Sat, 24 Jan 2009 18:25:19 +0100

The DVD recordings of the Tutorials presented at M&M 2008
are now available. They are $15.00 plus shipping. I can accept
checks, credit cards and institutional PO's. I prefer orders by
email or snail mail. Telephone orders are also accepted, but they
make me cranky and prone to errors.
The full instructions for ordering are at this web address
http://microscopy.org/MSAUnits/Education/VideoCatalogue.html.

Please request Priority Mail if you are in a hurry. Otherwise
they go by Media Mail or First Class mail, whichever is lowest.


The following are the new titles:

307 - Cryo-Fluorescence: A Tool for Correlative Cryo-Light and
Cryo-Electron Microscopy, Presented by Cindi L. Schwartz

308 - Live Cell Imaging Limitations. Presented by Simon Watkins

309 - Electron Backscatter Diffraction: Operation and
Applications. Presented by: David Field

310 Electron-Probe Microanalysis (EPMA): An Overview for
Beginners
and a Status Report for Experts. Presented by: Paul Carpenter

311- Lorentz Microscopy -- A Versatile Technique for Studying
Magnetic Multilayers, Elements and Nanowires Presented by : John
Chapman

312-Stereological Characterization of the Geometry of Three
Dimensional Microstructures. Presented by: Robert. T. DeHoff

313 ???Image J, A Useful Tool for Image Processing and Analysis.
Presented by : Joel B. Sheffield

314 Job Hunting for Scientific Professionals. Presented by : Bev
Maleeff

Greg Erdos
5410 SE 185th Ave
Micanopy FL 32667
gwe-at-ufl.edu
--
Greg Erdos
Assistant Director Emeritus
Biotechnology Program, UF
Micanopy FL


==============================Original Headers==============================
14, 24 -- From gwe-at-ufl.edu Sat Jan 24 14:15:38 2009
14, 24 -- Received: from smtp.ufl.edu (smtp04.osg.ufl.edu [128.227.74.71])
14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0OKFcDo014693
14, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Jan 2009 14:15:38 -0600
14, 24 -- Received: from osgjas01.cns.ufl.edu (osgjas01.cns.ufl.edu [128.227.74.131])
14, 24 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n0OKFbNq014792
14, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Jan 2009 15:15:37 -0500
14, 24 -- Message-ID: {1698358944.165111232828137648.JavaMail.osg-at-osgjas01.cns.ufl.edu}
14, 24 -- Date: Sat, 24 Jan 2009 15:15:37 -0500 (EST)
14, 24 -- From: gregerdos {gwe-at-ufl.edu}
14, 24 -- Reply-To: gwe-at-ufl.edu
14, 24 -- To: Microscopy-at-microscopy.com
14, 24 -- Subject: New Tutorial DVDs
14, 24 -- MIME-Version: 1.0
14, 24 -- Content-Type: text/plain; format=flowed; charset=us-ascii
14, 24 -- Content-Transfer-Encoding: 7bit
14, 24 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
14, 24 -- X-Originating-IP: 74.178.52.20 [74.178.52.20]
14, 24 -- X-Virus-Scanned: ClamAV 0.92/8899/Sat Jan 24 08:06:33 2009 on mailfilter05.cns.ufl.edu
14, 24 -- X-Virus-Status: Clean
14, 24 -- X-Spam-Status: hits=0.1, required=5, tests=RDNS_NONE
14, 24 -- X-UFL-Spam-Status: hits=0.1, required=5, tests=RDNS_NONE
14, 24 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
14, 24 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
==============================End of - Headers==============================

From hmasuno-at-ehime-chs.ac.jp Sat Jan 24 15:23:16 2009
Return-Path: {hmasuno-at-ehime-chs.ac.jp}
Received: from google.com (host86-162-215-141.range86-162.btcentralplus.com [86.162.215.141])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0OLNFnc003057
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 24 Jan 2009 15:23:15 -0600
Received: from [84.193.55.86] (HELO google.com)
by icy-dipthebill.cn; Sat, 24 Jan 2009 21:23:07 +0000

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: emer.ryan-at-dit.ie
Name: Emer Ryan

Organization: CREST DIT DUBLIN

Title-Subject: [Filtered] Looking for carbon

Question: Hello all,

I have been given a sample to analyse. The sample is non conducting
and are sputtered with gold. I've run an EDX and found various
elements,nothing too interesting, but the spectrum includes carbon. I
have explained to the requester that although carbon is present, I
cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is
depositing carbon during analysis. I note that folk are growing
carbon whiskers on AFM tips to produce sharper tips but putting the
tip in a SEM for a few minutes; the older the SEM, the better i.e.
the SEM deposits carbon.....
What is the general opinion regarding detecting carbon using EDX?
Appreciated,
Emer.




Login Host: 147.252.66.48
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 2009
10, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QDvfm8009249
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 07:57:42 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]}
10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Looking for carbon
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From itas-at-jumpy.it Mon Jan 26 08:27:12 2009
Return-Path: {itas-at-jumpy.it}
Received: from google.com ([89.251.42.166])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QERBWK023851
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 26 Jan 2009 08:27:11 -0600
Received: from [101.62.91.135] (HELO google.com)
by misty-jakeloo.co.uk; Mon, 26 Jan 2009 15:28:52 +0100
Message-ID: {00000003CE00E892016095919}
Reply-To: Tansy Keilbach {1239boler.reene-at-gmail.com}



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie]
} Sent: Monday, January 26, 2009 7:59 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: Looking for carbon
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both emer.ryan-at-dit.ie as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: emer.ryan-at-dit.ie
} Name: Emer Ryan
}
} Organization: CREST DIT DUBLIN
}
} Title-Subject: [Filtered] Looking for carbon
}
} Question: Hello all,
}
} I have been given a sample to analyse. The sample is non
} conducting and are sputtered with gold. I've run an EDX and
} found various elements,nothing too interesting, but the
} spectrum includes carbon. I have explained to the requester
} that although carbon is present, I cannot discount that the
} EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during
} analysis. I note that folk are growing carbon whiskers on AFM
} tips to produce sharper tips but putting the tip in a SEM for
} a few minutes; the older the SEM, the better i.e.
} the SEM deposits carbon.....
} What is the general opinion regarding detecting carbon using EDX?
} Appreciated,
} Emer.
}
}
}
}
} Login Host: 147.252.66.48
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43
} 2009 10, 11 -- Received: from [206.69.208.22]
} (msdvpn8.msd.anl.gov [130.202.238.72])
} 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0QDvfm8009249
} 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan
} 2009 07:57:42 -0600
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]}
} 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 10, 11 -- To:
} microscopy-at-microscopy.com 10, 11 -- From: emer.ryan-at-dit.ie
} (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW:
} Looking for carbon 10, 11 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Mon Jan 26 12:24:16 2009
7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QIOFP0000862
7, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 12:24:15 -0600
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Mon, 26 Jan 2009 12:24:14 -0600
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] viaWWW: Looking for carbon
7, 25 -- Date: Mon, 26 Jan 2009 12:23:56 -0600
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BE-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200901261358.n0QDweMe010127-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] viaWWW: Looking for carbon
7, 25 -- thread-index: Acl/vjH5z202usteT22licQ7ReHP+QAJP/Eg
7, 25 -- References: {200901261358.n0QDweMe010127-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {emer.ryan-at-dit.ie} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 26 Jan 2009 18:24:14.0774 (UTC) FILETIME=[4AE73560:01C97FE3]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QIOFP0000862
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Mon, 26 Jan 2009 12:30:43 -0600
Subject: [Microscopy] RE: viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Emer,

Why did you use EDS when you have probe, presumably with WDS? WDS is
much better for detection of carbon. If you suspect you detect deposited
carbon, you can see (with WDS) if carbon peak is growing with time.
Besides, most probes with diffusion pumps are equipped with liquid
nitrogen trap. If you have one, it can decrease rate of carbon
deposition dramatically.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie]
} Sent: Monday, January 26, 2009 7:59 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: Looking for carbon
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both emer.ryan-at-dit.ie as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: emer.ryan-at-dit.ie
} Name: Emer Ryan
}
} Organization: CREST DIT DUBLIN
}
} Title-Subject: [Filtered] Looking for carbon
}
} Question: Hello all,
}
} I have been given a sample to analyse. The sample is non
} conducting and are sputtered with gold. I've run an EDX and
} found various elements,nothing too interesting, but the
} spectrum includes carbon. I have explained to the requester
} that although carbon is present, I cannot discount that the
} EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during
} analysis. I note that folk are growing carbon whiskers on AFM
} tips to produce sharper tips but putting the tip in a SEM for
} a few minutes; the older the SEM, the better i.e.
} the SEM deposits carbon.....
} What is the general opinion regarding detecting carbon using EDX?
} Appreciated,
} Emer.
}
}
}
}
} Login Host: 147.252.66.48
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43
} 2009 10, 11 -- Received: from [206.69.208.22]
} (msdvpn8.msd.anl.gov [130.202.238.72])
} 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n0QDvfm8009249
} 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan
} 2009 07:57:42 -0600
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]}
} 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 10, 11 -- To:
} microscopy-at-microscopy.com 10, 11 -- From: emer.ryan-at-dit.ie
} (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW:
} Looking for carbon 10, 11 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Mon Jan 26 12:30:43 2009
8, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QIUg7w006964
8, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 12:30:42 -0600
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Mon, 26 Jan 2009 12:30:41 -0600
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="us-ascii"
8, 25 -- Subject: RE: [Microscopy] viaWWW: Looking for carbon
8, 25 -- Date: Mon, 26 Jan 2009 12:30:20 -0600
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BF-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200901261358.n0QDweMe010127-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] viaWWW: Looking for carbon
8, 25 -- thread-index: Acl/vjH5z202usteT22licQ7ReHP+QAJd3fw
8, 25 -- References: {200901261358.n0QDweMe010127-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {emer.ryan-at-dit.ie} , {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 26 Jan 2009 18:30:41.0740 (UTC) FILETIME=[318D88C0:01C97FE4]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QIUg7w006964
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 26 Jan 2009 13:44:12 -0600
Subject: [Microscopy] Re: viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is the specimen sitting on a Carbon sticky tab?
If so, and your beam gets close or on it, you will
get C.

C is so ubiquitous that I've rarely seen a specta
that does not have some amount of C. Your coater
is probably an oil diaphragm pumped system and will
back flush some amount of hydrocarbon into the chamber
and onto the specimen. Then, taking the specimen out
of the coater and exposing it to atmosphere (again) will
put more C on it. Then, into the SEM chamber. If roughing
pump is oil dual vane mechanical, then likely some back
streaming there.

You can try some experiments to baseline your inherent C.

1. Take a clean, unused Al stub and put in SEM. Run at
5KV and collect a spectra. Save it. Then do this at 10KV
and save, 15KV and save 20KV and save. At this point, you
ought not see polymerization squares/rectangles from the beam.

2. Take another clean, used Al stub and coat it normally.
Do the same runs as in #1.

3. Note if you get polymerization patterns.

4. The C from #1 is arguably your background C and can be
subtracted from specimen spectra readings. Be sure to collect
for the same time, same WD, same DT and same probe conditions.
For simple situations, I collect for 60 seconds. For more
critical applications, I use 120 seconds. At the standard 10eV
per bin, the longer time fills in the peaks and I think improves
overall results. Plus it eliminates the collection time variable
and standardizes the collection by at least that one variable.

5. Get a stainless set of standards and check them at different
KV and see what C you get for each. subtract the background
and check correlation for the standard.

C is all over the place so it is hard to make disappear. Based
on some communications with an MSA lister, I also noted that O
is a joker.

Light element analysis is tricky. Depending on the specimen,
I would analyze at 5KV and collect spectra. Then move up the KV.
As more of the heavier elements' main peaks show up, the quant
will change.

A suggestion. See if it works for you. Sample storage is also
a big issue (though it did not occur to me until a couple of
years ago, sigh). Store samples under vacuum or purge with N2.
A Sample Saver is a great way to do this since you can purge it
or pump it with the stubs in place and then close it down. Or
you can store specimens in a vacuum oven. Sometimes I don't
particularly like the ovens when heated since oxides tend to
form immediately upon removal. This is a killer for EBSD.

Let us know how it goes. C is a constant battle for me. Glad
I'm not alone. I Probably could write more but then it takes
on a short novel!

gary g.




At 05:59 AM 1/26/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
21, 20 -- From gary-at-gaugler.com Mon Jan 26 13:44:11 2009
21, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
21, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0QJiBCk031268
21, 20 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 13:44:11 -0600
21, 20 -- Message-Id: {200901261944.n0QJiBCk031268-at-ns.microscopy.com}
21, 20 -- Received: (qmail 1662 invoked from network); 26 Jan 2009 11:39:15 -0800
21, 20 -- Received: by simscan 1.1.0 ppid: 1659, pid: 1660, t: 0.1074s
21, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
21, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
21, 20 -- by smtp1 with SMTP; 26 Jan 2009 11:39:14 -0800
21, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
21, 20 -- Date: Mon, 26 Jan 2009 11:44:07 -0800
21, 20 -- To: emer.ryan-at-dit.ie
21, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
21, 20 -- Subject: Re: [Microscopy] viaWWW: Looking for carbon
21, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
21, 20 -- In-Reply-To: {200901261359.n0QDxuN5011708-at-ns.microscopy.com}
21, 20 -- References: {200901261359.n0QDxuN5011708-at-ns.microscopy.com}
21, 20 -- Mime-Version: 1.0
21, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: ray.twesten-at-sbcglobal.net
Date: Mon, 26 Jan 2009 14:03:23 -0600
Subject: [Microscopy] viaWWW: Multiple EELS acquisition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Rao,
There is a script that will do what you need in the DM Script Database
maintained by TU-Graz. The URL is:
http://www.felmi-zfe.tugraz.at/dm_scripts/dm_scripts/freeware/programs/Multi
ple-EELS-Acquisition.htm

This script was written by David Mitchell. I have not tried it personally,
but David's scripts tend to be of the highest quality, work out of the box,
and are typically easy to follow and modify if needed. The description of
the script follows:

Multiple EELS Acquisition
script version: 1.4 platform: PC DM version: 3.7.x modified: 2005-02-08
hardware:JEOL 2010F, GIF 2000
contact: David Mitchell (ANSTO Materials)

description / instruction
This script will capture multiple EELS spectra and store them in a 3D EELS
data cube. The EELS data cube is then processed to sum the spectra.
Alignment and summation of spectra can be done with or without energy shift
correction (to compensate for any energy drift). EELS data cubes can also be
processed offline using the script 'EELS Cube Data Extraction', available at
this site. Offline processing permits individual spectra to be extracted and
spectra to be omitted from the summation. To use this script set up the
spectrometer to display an EELS spectrum on the CCD - then run the script -
enter exposure time, number of frames and any delay between frame capture.
To remove X-ray spikes from the data, use the script 'EELS Cube Data
Editor'.


The URL for the TU-Graz database is:
http://www.felmi-zfe.tugraz.at/dm_scripts/welcome.html


This database is not supported or maintained by Gatan. It is provided as a
community service by the kind folks at FELMI-ZFE

Best regards,
Ray


Ray D. Twesten, Ph.D.
Product Manager – Analytical Instruments
  Gatan, Inc.
Tel. +1 (925) 224-7392


-----Original Message-----
X-from: rao.karavadi-at-rutgers.edu [mailto:rao.karavadi-at-rutgers.edu]
Sent: Friday, January 23, 2009 3:32 PM
To: ray.twesten-at-sbcglobal.net

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rao.karavadi-at-rutgers.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rao.karavadi-at-rutgers.edu
Name: Rao Karavadi

Organization: Rutgers University

Title-Subject: [Filtered] Multiple EELS acquisition

Question: Hi all

I am looking for a Digital Micrograph script file, where we can
acquire Multiple EELS spectrum with regular intervals of time. I am
interested to study radiation damage by Electron beam on different
materials which requires collections of EELS spectra with regular
intervals of time.

I truly appreciate your response

Thanks
Rao Karavadi
Rutgers: The State University of New Jersey
607 Taylor Road
Piscataway, NJ 08854
USA


Login Host: 128.6.64.150
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Fri Jan 23 17:19:35 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n0NNJY7e015671
10, 11 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 17:19:34
-0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c5a000f2a6dd-at-[206.69.208.22]}
10, 11 -- Date: Fri, 23 Jan 2009 17:19:33 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: rao.karavadi-at-rutgers.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Multiple EELS acquisition
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
28, 28 -- From ray.twesten-at-sbcglobal.net Mon Jan 26 14:03:23 2009
28, 28 -- Received: from smtp126.sbc.mail.sp1.yahoo.com (smtp126.sbc.mail.sp1.yahoo.com [69.147.65.185])
28, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0QK3Nrx013115
28, 28 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 14:03:23 -0600
28, 28 -- Received: (qmail 66317 invoked from network); 26 Jan 2009 20:03:22 -0000
28, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
28, 28 -- s=s1024; d=sbcglobal.net;
28, 28 -- h=Received:X-Yahoo-Newman-Property:From:To:Cc:References:In-Reply-To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language;
28, 28 -- b=fxzWWh25fIOcWxHequgnqJ4tussZVz8ASIHrz4TOXJFHVJd+is6zONdeIRgiOUsqoNqGizBddPh45xcwiwOfAm2UOWYZUafZoYLYmkZlM/O63sRp55yQVXUgfVDW28SoFwAeFeT3bdmgdpCJtdVqvULV471FSzTaG306Qaxcv5c= ;
28, 28 -- Received: from unknown (HELO TWESTEDLTE6400) (ray.twesten-at-209.3.42.11 with login)
28, 28 -- by smtp126.sbc.mail.sp1.yahoo.com with SMTP; 26 Jan 2009 20:03:21 -0000
28, 28 -- X-Yahoo-Newman-Property: ymail-3
28, 28 -- From: "Ray Twesten" {ray.twesten-at-sbcglobal.net}
28, 28 -- To: {rao.karavadi-at-rutgers.edu}
28, 28 -- Cc: {microscopy-at-microscopy.com}
28, 28 -- References: {200901232332.n0NNWKJl027316-at-ns.microscopy.com}
28, 28 -- In-Reply-To: {200901232332.n0NNWKJl027316-at-ns.microscopy.com}
28, 28 -- Subject: RE: [Microscopy] viaWWW: Multiple EELS acquisition
28, 28 -- Date: Mon, 26 Jan 2009 12:03:21 -0800
28, 28 -- Message-ID: {005101c97ff1$23bfd470$6b3f7d50$-at-twesten-at-sbcglobal.net}
28, 28 -- MIME-Version: 1.0
28, 28 -- Content-Type: text/plain;
28, 28 -- charset="iso-8859-1"
28, 28 -- X-Mailer: Microsoft Office Outlook 12.0
28, 28 -- Thread-Index: Acl9stZQ79BBLNyCSr6oeJjwX1+88wCPGPAA
28, 28 -- Content-Language: en-us
28, 28 -- Content-Transfer-Encoding: 8bit
28, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QK3Nrx013115
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Mon, 26 Jan 2009 14:17:29 -0600
Subject: [Microscopy] viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would run comparative samples to verify or disprove your findings. I
would try to find samples as similar in nature as possible, but even if
you can't match textures you could compare the chemistry.

You could examine graphite as one extreme, polymers such as polyethylene
or polystyrene, and glass or metals as the other extreme.

The peak-to-background ratios are important. I would encourage scaling
the spectra to match the background intensities. You could use the
spectrum from graphite to see what 100% carbon should look like. The
polyethylene might show a little less carbon since it has hydrogen which
you cannot see. Glass or pure metals should not have any carbon, so the
only carbon you see there should be that due to contamination build up.
And build-up will grow with time. You should be able to compare
successive spectra and see growth in the carbon peak.

I would offer those results to your client and see what most resembles
their sample. If they have only low levels of carbon in their spectra,
it may be hard to say much about the source of that carbon. Maybe it was
really present. Maybe it was contamination from the scope. Maybe it was
contamination from the sample. Give it a try and see what happens.

Warren S.

-----Original Message-----
X-from: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie]
Sent: Monday, January 26, 2009 7:59 AM
To: wesaia-at-iastate.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both emer.ryan-at-dit.ie as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: emer.ryan-at-dit.ie
Name: Emer Ryan

Organization: CREST DIT DUBLIN

Title-Subject: [Filtered] Looking for carbon

Question: Hello all,

I have been given a sample to analyse. The sample is non conducting
and are sputtered with gold. I've run an EDX and found various
elements,nothing too interesting, but the spectrum includes carbon. I
have explained to the requester that although carbon is present, I
cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is
depositing carbon during analysis. I note that folk are growing
carbon whiskers on AFM tips to produce sharper tips but putting the
tip in a SEM for a few minutes; the older the SEM, the better i.e.
the SEM deposits carbon.....
What is the general opinion regarding detecting carbon using EDX?
Appreciated,
Emer.




Login Host: 147.252.66.48
------------------------------------------------------------------------
---

==============================Original
Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 2009
10, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov
[130.202.238.72])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0QDvfm8009249
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009
07:57:42 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]}
10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Looking for carbon
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
21, 36 -- From wesaia-at-iastate.edu Mon Jan 26 14:17:28 2009
21, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QKHRLV027938
21, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 14:17:27 -0600
21, 36 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
21, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n0QKHMWO004962;
21, 36 -- Mon, 26 Jan 2009 14:17:22 -0600
21, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
21, 36 -- id 075e_6ff3e3fe_ebe4_11dd_86cf_00137253420a;
21, 36 -- Mon, 26 Jan 2009 14:03:45 -0600
21, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
21, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n0QKHH0i031785;
21, 36 -- Mon, 26 Jan 2009 14:17:17 -0600
21, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
21, 36 -- Mon, 26 Jan 2009 14:17:21 -0600
21, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 36 -- Content-class: urn:content-classes:message
21, 36 -- MIME-Version: 1.0
21, 36 -- Content-Type: text/plain;
21, 36 -- charset="us-ascii"
21, 36 -- Subject: RE: [Microscopy] viaWWW: Looking for carbon
21, 36 -- Date: Mon, 26 Jan 2009 14:20:09 -0600
21, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270395C4AA-at-maire.eng.iastate.edu}
21, 36 -- In-Reply-To: {200901261358.n0QDwk2H010314-at-ns.microscopy.com}
21, 36 -- X-MS-Has-Attach:
21, 36 -- X-MS-TNEF-Correlator:
21, 36 -- Thread-Topic: [Microscopy] viaWWW: Looking for carbon
21, 36 -- Thread-Index: Acl/vjafmPdzmZWCQJSc6DzJb3u+swAM4Iug
21, 36 -- References: {200901261358.n0QDwk2H010314-at-ns.microscopy.com}
21, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
21, 36 -- To: {emer.ryan-at-dit.ie} , {Microscopy-at-microscopy.com}
21, 36 -- X-OriginalArrivalTime: 26 Jan 2009 20:17:21.0806 (UTC) FILETIME=[184A1AE0:01C97FF3]
21, 36 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.1.26.200149
21, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0'
21, 36 -- Content-Transfer-Encoding: 8bit
21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QKHRLV027938
==============================End of - Headers==============================




From: Don.Becker-at-bruker-axs.com
Date: Mon, 26 Jan 2009 15:19:12 -0600
Subject: [Microscopy] Immediate Sales Opening at Bruker AXS Microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bruker AXS Inc., a leading global provider of advanced X-ray solutions for the life and advanced materials sciences, is seeking an experienced
Regional Sales Manager - Microanalysis for the Southeast Territory

The Regional Sales Manager secures sales for BAXS Microanalysis Products in the southeast U.S. and act as company rep to existing customer base and prospective customers. Responsibilities include: prospecting for new customers, following up sales leads provided by the company, presenting and demonstrating company products, supplying quotations and technical information, formulating sales strategies, as well as negotiating and securing sales orders. In addition, the Regional Sales Manager will maintain contact with existing customers to assure their satisfaction, develop good working relationships with OEM salespeople, collect and report market information and provide routine sales forecasts.

Territory includes NC, SC, GA, FL, TN, AL, MS, AR, and LA. At least 50% travel is required. Bachelor's degree (B.S.or B.A.) from four-year college or university; or five years experience and/or training; or equivalent combination of education and experience. Three to five years experience in scientific equipment sales is a must. This position requires excellent verbal communication and interaction skills.

Bruker AXS Inc. offers a competitive salary and comprehensive benefits package including health and dental insurance, Flexible Spending Account, company sponsored life and disability insurance, 401(k) plan with company matching components, stock option plan, and vacation and sick/personal days.

Qualified applicants should submit resume and salary requirements in confidence to:


Bruker AXS Inc.
Attn: Don Becker, Sales Manager
1239 Parkway Ave. Suite 203
Ewing, NJ 08628
FAX#: 609-771-4411
E-mail: don.becker-at-bruker-axs.com

Equal Opportunity Employer


------------------------------------------------------------------------------------
Don Becker
U.S. Sales Manager - Microanalysis
Bruker AXS Inc.
1239 Parkway Avenue, Suite 203
Ewing, NJ 08628
Tel: +1 (609) 771-4400
Fax: +1 (609) 771-4411
don.becker-at-bruker-axs.com
www.bruker-axs.com
------------------------------------------------------------------------------------



==============================Original Headers==============================
11, 28 -- From Don.Becker-at-bruker-axs.com Mon Jan 26 15:19:11 2009
11, 28 -- Received: from mail1.bruker-axs.com (mail2.bruker-axs.com [12.18.13.126])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QLJAen017452
11, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 15:19:11 -0600
11, 28 -- Received: from mail1.bruker-axs.com ([172.16.0.32]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959);
11, 28 -- Mon, 26 Jan 2009 15:19:29 -0600
11, 28 -- X-Ninja-PIM: Scanned by Ninja
11, 28 -- X-Ninja-AttachmentFiltering: (no action)
11, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959);
11, 28 -- Mon, 26 Jan 2009 15:19:07 -0600
11, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by
11, 28 -- msnmail1.bruker-axs.com ([172.16.0.53]) with mapi; Mon, 26 Jan 2009 15:19:06
11, 28 -- -0600
11, 28 -- From: "Becker, Don" {Don.Becker-at-bruker-axs.com}
11, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
11, 28 -- Date: Mon, 26 Jan 2009 15:19:05 -0600
11, 28 -- Subject: Immediate Sales Opening at Bruker AXS Microanalysis
11, 28 -- Thread-Topic: Immediate Sales Opening at Bruker AXS Microanalysis
11, 28 -- Thread-Index: Acl/+7glyZxdzV9LSri0uywtf0YPAA==
11, 28 -- Message-ID: {6CBB09E530A398488E07976781F692030FB0A14909-at-msnmail1.bruker-axs.com}
11, 28 -- Accept-Language: en-US
11, 28 -- Content-Language: en-US
11, 28 -- acceptlanguage: en-US
11, 28 -- Content-Type: text/plain; charset="iso-8859-1"
11, 28 -- MIME-Version: 1.0
11, 28 -- X-OriginalArrivalTime: 26 Jan 2009 21:19:07.0254 (UTC) FILETIME=[B8E89960:01C97FFB]
11, 28 -- Content-Transfer-Encoding: 8bit
11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QLJAen017452
==============================End of - Headers==============================




From: nicholas.ritchie-at-nist.gov
Date: Mon, 26 Jan 2009 16:01:34 -0600
Subject: [Microscopy] Announcing the MAS Microanalysis of Particles Topical Conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

*************************************************
* MICROBEAM ANALYSIS SOCIETY *
* *
* Microanalysis of Particles 2009 *
* Topical Conference *
* *
* April 21-23, 2009 *
* Westmont, Il *
* *
************************************************

This conference takes a pragmatic approach to a
subtle subject - the microanalysis of particles.
Techniques covered include EDS, WDS, AEM, SIMS,
ESCA, CL, XRD, synchrotron XRF. We have invited
some of the most well known names in the industry
to present extended talks in the morning. In
the afternoon, attendees will be able to select
among various hands-on modules. Both novices
and experienced analysts will benefit.

For additional details:

http://www.microbeamanalysis.org/meetings/topical/Particles2009/program.htm

To sign up:

http://www.microbeamanalysis.org/meetings/topical/Particles2009/registration.htm

----------------------------------------------------------------------

Confirmed Invited Speakers:

Bob Anderhalt, EDAX - Improved quantitative analysis of particles with
topography using multiple EDS detectors

John Armstrong, Carnegie Institution for Science - ZAF corrections for
particle analysis

Paul Carpenter, Washington University of St. Louis - Electron-probe
microanalysis of particles and heterogeneous materials

John Fournelle, University of Wisconsin - Madison - Evaluating
atmospheric particles with combination of EDS, WDS and EBSD techniques

Nicholas Ritchie, NIST - Using DTSA-II to simulate and interpret
energy dispersive spectra from particles

Volker Rose, Argonne National Laboratory - New ways to see a smaller
world: The Hard X-ray Nanoprobe at Argonne National Laboratory

Elaine Schumacher, McCrone Associates, Inc. - Transmission electron
microscopy for high resolution single-particle analysis

Craig Schwandt, McCrone Associates, Inc. - Microanalysis of particles:
An overview

Joseph Swider, McCrone Associates, Inc. - Powder micro X-ray
diffraction of particles

Ed Vicenzi, Smithsonian Institution - Microanalysis of inclusions

Robert Winarski, Argonne National Laboratory - The X-ray nanoprobe

Nestor Zaluzec, Argonne National Laboratory - Characterization of
nanoscale particles in the analytical electron microscope: Past, present
and future


==============================Original Headers==============================
19, 22 -- From nicholas.ritchie-at-nist.gov Mon Jan 26 16:01:34 2009
19, 22 -- Received: from smtp.nist.gov (rimp2.nist.gov [129.6.16.227])
19, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QM1XHU001966
19, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 16:01:34 -0600
19, 22 -- Received: from smsd-fw.nist.gov (smsd-fw.nist.gov [129.6.126.23])
19, 22 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id n0QM1Oc8023808
19, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 17:01:24 -0500
19, 22 -- Received: from no.name.available by smsd-fw.nist.gov
19, 22 -- via smtpd (for smtpint.nist.gov [129.6.16.228]) with ESMTP; Mon, 26 Jan 2009 17:06:20 -0500
19, 22 -- Message-ID: {497E32B4.7060101-at-nist.gov}
19, 22 -- Date: Mon, 26 Jan 2009 17:01:24 -0500
19, 22 -- From: Nicholas Ritchie {nicholas.ritchie-at-nist.gov}
19, 22 -- Reply-To: nicholas.ritchie-at-nist.gov
19, 22 -- Organization: NIST
19, 22 -- User-Agent: Thunderbird 2.0.0.18 (Macintosh/20081105)
19, 22 -- MIME-Version: 1.0
19, 22 -- To: Microscopy-at-microscopy.com
19, 22 -- Subject: Announcing the MAS Microanalysis of Particles Topical Conference
19, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
19, 22 -- Content-Transfer-Encoding: 7bit
19, 22 -- X-NIST-MailScanner: Found to be clean
19, 22 -- X-NIST-MailScanner-From: nicholas.ritchie-at-nist.gov
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 26 Jan 2009 17:05:48 -0600
Subject: [Microscopy] viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pls change #2 to "... un-used Al stub ..."

gary g.

At 11:45 AM 1/26/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
7, 20 -- From gary-at-gaugler.com Mon Jan 26 17:05:48 2009
7, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0QN5lAg019325
7, 20 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 17:05:47 -0600
7, 20 -- Message-Id: {200901262305.n0QN5lAg019325-at-ns.microscopy.com}
7, 20 -- Received: (qmail 28562 invoked from network); 26 Jan 2009 15:00:51 -0800
7, 20 -- Received: by simscan 1.1.0 ppid: 28555, pid: 28556, t: 0.1671s
7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
7, 20 -- by smtp1 with SMTP; 26 Jan 2009 15:00:51 -0800
7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
7, 20 -- Date: Mon, 26 Jan 2009 15:05:44 -0800
7, 20 -- To: gary-at-gaugler.com
7, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Looking for carbon
7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
7, 20 -- In-Reply-To: {200901261945.n0QJjcZB001291-at-ns.microscopy.com}
7, 20 -- References: {200901261945.n0QJjcZB001291-at-ns.microscopy.com}
7, 20 -- Mime-Version: 1.0
7, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: y.han-at-sheffield.ac.uk
Date: Tue, 27 Jan 2009 15:49:22 -0600
Subject: [Microscopy] viaWWW: preparation of plan-view TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: y.han-at-sheffield.ac.uk
Name: Yisong Han

Organization: University of Sheffield

Title-Subject: [Filtered] preparation of plan-view TEM samples

Question: Dear All,

Does anybody know how to protect plan-view TEM samples, which are
thinned from one side only, from contamination during ion beam
milling? Thanks very much in advance.

Yisong

Login Host: 143.167.204.169
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]}
8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: aochalsk-at-uottawa.ca
Date: Tue, 27 Jan 2009 15:49:54 -0600
Subject: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX confocal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both aochalsk-at-uottawa.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: aochalsk-at-uottawa.ca
Name: Andrew Ochalski

Organization: University of Ottawa

Title-Subject: [Filtered] Hardware/Software for Olympus Fluoview FVX confocal

Question: Hi all,

Four years ago, we were given an older confocal microscope for
educational purposes, specifically an Olympus Fluoview FVX. The
software, Fluoview 2.1 as well as the scan/stage controller and scan
head all work together in a Win NT environment. We have none of the
original software disks and the (older) PC on which the whole lot is
running is constantly crashing (blue screen of death)and is probably
not long for this world. Olympus used to sell an upgrade package
compatible with WinXP, but Olympus is out of them and they are not
being made any more. If anyone has an upgraded system that they are
no longer using or, for any reason have a version of the controller,
card and updated software that they would consider selling (or giving
away), we would be thrilled to hear from you.

Andrew Ochalski,
Technical Supervisor,
Carsen Advanced Educational Microscopy Resource Centre
Biology Department
University of Ottawa
30 Marie Curie
Ottawa, ON
CANADA
K1N 6N5



Login Host: 137.122.148.65
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:53 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnq5V018607
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:53 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c5a531eb8978-at-[206.69.208.22]}
10, 11 -- Date: Tue, 27 Jan 2009 15:49:52 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: aochalsk-at-uottawa.ca (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Hardware/Software for Olympus Fluoview FVX confocal
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Tue, 27 Jan 2009 16:26:54 -0600
Subject: [Microscopy] Re: viaWWW: preparation of plan-view TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Evaporate salt (NaCl) on the side you want to protect. Dissolve the salt
in water when you are finished ion milling and the contamination will
flush away.

Ron Anderson

y.han-at-sheffield.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: y.han-at-sheffield.ac.uk
} Name: Yisong Han
}
} Organization: University of Sheffield
}
} Title-Subject: [Filtered] preparation of plan-view TEM samples
}
} Question: Dear All,
}
} Does anybody know how to protect plan-view TEM samples, which are
} thinned from one side only, from contamination during ion beam
} milling? Thanks very much in advance.
}
} Yisong
}
} Login Host: 143.167.204.169
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216
} 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]}
} 8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}
}


==============================Original Headers==============================
4, 19 -- From randerson20-at-tampabay.rr.com Tue Jan 27 16:26:54 2009
4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMQr5q014197
4, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Jan 2009 16:26:54 -0600
4, 19 -- Received: from [127.0.0.1] (really [24.73.73.214])
4, 19 -- by hrndva-omta06.mail.rr.com with ESMTP
4, 19 -- id {20090127222653.TIOS10307.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} ;
4, 19 -- Tue, 27 Jan 2009 22:26:53 +0000
4, 19 -- Message-ID: {497F8A24.9020105-at-tampabay.rr.com}
4, 19 -- Date: Tue, 27 Jan 2009 17:26:44 -0500
4, 19 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
4, 19 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
4, 19 -- MIME-Version: 1.0
4, 19 -- To: y.han-at-sheffield.ac.uk, Listserver {Microscopy-at-Microscopy.Com}
4, 19 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples
4, 19 -- References: {200901272149.n0RLnXJs018374-at-ns.microscopy.com}
4, 19 -- In-Reply-To: {200901272149.n0RLnXJs018374-at-ns.microscopy.com}
4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jsiegmund-at-7thwavelabs.com
Date: Tue, 27 Jan 2009 16:52:57 -0600
Subject: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Email: jsiegmund-at-7thwavelabs.com
Name: Joachim Siegmund
Organization: SeventhWave Labs

On the software side, ImageJ with the UCSD plugin might work for you:
http://rsb.info.nih.gov/ij/plugins/ucsd.html

On the driver side, you can try whether or not the win2000 drivers work
with winXP ... sometimes they do. There is also a compatibility mode in
XP for old software, I think.

Joachim


-----Original Message-----
X-from: aochalsk-at-uottawa.ca [mailto:aochalsk-at-uottawa.ca]
Sent: Tuesday, January 27, 2009 4:02 PM
To: Joachim Siegmund

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both aochalsk-at-uottawa.ca as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: aochalsk-at-uottawa.ca
Name: Andrew Ochalski

Organization: University of Ottawa

Title-Subject: [Filtered] Hardware/Software for Olympus Fluoview FVX
confocal

Question: Hi all,

Four years ago, we were given an older confocal microscope for
educational purposes, specifically an Olympus Fluoview FVX. The
software, Fluoview 2.1 as well as the scan/stage controller and scan
head all work together in a Win NT environment. We have none of the
original software disks and the (older) PC on which the whole lot is
running is constantly crashing (blue screen of death)and is probably
not long for this world. Olympus used to sell an upgrade package
compatible with WinXP, but Olympus is out of them and they are not
being made any more. If anyone has an upgraded system that they are
no longer using or, for any reason have a version of the controller,
card and updated software that they would consider selling (or giving
away), we would be thrilled to hear from you.

Andrew Ochalski,
Technical Supervisor,
Carsen Advanced Educational Microscopy Resource Centre
Biology Department
University of Ottawa
30 Marie Curie
Ottawa, ON
CANADA
K1N 6N5



Login Host: 137.122.148.65
------------------------------------------------------------------------
---

==============================Original
Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:53 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n0RLnq5V018607
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009
15:49:53 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c5a531eb8978-at-[206.69.208.22]}
10, 11 -- Date: Tue, 27 Jan 2009 15:49:52 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: aochalsk-at-uottawa.ca (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Hardware/Software for Olympus Fluoview FVX
confocal
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================

This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer


==============================Original Headers==============================
22, 29 -- From jsiegmund-at-7thwavelabs.com Tue Jan 27 16:52:57 2009
22, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136])
22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMqvJ3028688
22, 29 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 16:52:57 -0600
22, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1])
22, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id EF4AD39800A;
22, 29 -- Tue, 27 Jan 2009 16:52:56 -0600 (CST)
22, 29 -- X-AuditID: c0a80218-a6841bb000000bbe-63-497f9048ba21
22, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29])
22, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 86013424013;
22, 29 -- Tue, 27 Jan 2009 16:52:56 -0600 (CST)
22, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
22, 29 -- Content-class: urn:content-classes:message
22, 29 -- MIME-Version: 1.0
22, 29 -- Content-Type: text/plain;
22, 29 -- charset="us-ascii"
22, 29 -- Subject: RE: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX confocal
22, 29 -- Date: Tue, 27 Jan 2009 16:52:56 -0600
22, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A660AC86-at-wave-mail.7thwave.local}
22, 29 -- X-MS-Has-Attach:
22, 29 -- X-MS-TNEF-Correlator:
22, 29 -- Thread-Topic: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX confocal
22, 29 -- Thread-Index: AcmAyvBuSyjctnk4REaRT06YHHto6QABbsAg
22, 29 -- References: {200901272202.n0RM2O5x012850-at-ns.microscopy.com}
22, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com}
22, 29 -- To: {aochalsk-at-uottawa.ca} , {microscopy-at-microscopy.com}
22, 29 -- X-Brightmail-Tracker: AAAAAQ1k0lE=
22, 29 -- Content-Transfer-Encoding: 8bit
22, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0RMqvJ3028688
==============================End of - Headers==============================




From: cljohnson33-at-gmail.com
Date: Tue, 27 Jan 2009 17:23:42 -0600
Subject: [Microscopy] viaWWW: preparation of plan-view TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here are a couple possibilities:

1. Paint the side you want to protect with a layer of clear fingernail
polish and dissolve in acetone after ion milling is finished.

2. I used to cut out a 3mm disk of a very thin sheet of mica and place
that under the specimen. Any redeposited material will stick to the
mica disk.

Craig.

-------------------------------
Craig L. Johnson
TEM Scientist
Centralized Research Facility
Drexel University College of Engineering
-------------------------------

}
} On Tue, Jan 27, 2009 at 5:31 PM, {randerson20-at-tampabay.rr.com} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Evaporate salt (NaCl) on the side you want to protect. Dissolve the salt
} } in water when you are finished ion milling and the contamination will
} } flush away.
} }
} } Ron Anderson
} }
} } y.han-at-sheffield.ac.uk wrote:
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at
} } } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} } } ---------------------------------------------------------------------------
} } } Remember this posting is most likely not from a Subscriber, so when replying
} } } please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
} } } ---------------------------------------------------------------------------
} } }
} } } Email: y.han-at-sheffield.ac.uk
} } } Name: Yisong Han
} } }
} } } Organization: University of Sheffield
} } }
} } } Title-Subject: [Filtered] preparation of plan-view TEM samples
} } }
} } } Question: Dear All,
} } }
} } } Does anybody know how to protect plan-view TEM samples, which are
} } } thinned from one side only, from contamination during ion beam
} } } milling? Thanks very much in advance.
} } }
} } } Yisong
} } }
} } } Login Host: 143.167.204.169
} } } ---------------------------------------------------------------------------
} } }
} } } ==============================Original Headers==============================
} } } 8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009
} } } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} } } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216
} } } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600
} } } 8, 11 -- Mime-Version: 1.0
} } } 8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]}
} } } 8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600
} } } 8, 11 -- To: microscopy-at-microscopy.com
} } } 8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
} } } 8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples
} } } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } } ==============================End of - Headers==============================
} } }
} } }
} } }
} }
} }
} } ==============================Original Headers==============================
} } 4, 19 -- From randerson20-at-tampabay.rr.com Tue Jan 27 16:26:54 2009
} } 4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
} } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMQr5q014197
} } 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Jan 2009 16:26:54 -0600
} } 4, 19 -- Received: from [127.0.0.1] (really [24.73.73.214])
} } 4, 19 -- by hrndva-omta06.mail.rr.com with ESMTP
} } 4, 19 -- id {20090127222653.TIOS10307.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} ;
} } 4, 19 -- Tue, 27 Jan 2009 22:26:53 +0000
} } 4, 19 -- Message-ID: {497F8A24.9020105-at-tampabay.rr.com}
} } 4, 19 -- Date: Tue, 27 Jan 2009 17:26:44 -0500
} } 4, 19 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
} } 4, 19 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
} } 4, 19 -- MIME-Version: 1.0
} } 4, 19 -- To: y.han-at-sheffield.ac.uk, Listserver {Microscopy-at-Microscopy.Com}
} } 4, 19 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples
} } 4, 19 -- References: {200901272149.n0RLnXJs018374-at-ns.microscopy.com}
} } 4, 19 -- In-Reply-To: {200901272149.n0RLnXJs018374-at-ns.microscopy.com}
} } 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 4, 19 -- Content-Transfer-Encoding: 7bit
} } ==============================End of - Headers==============================
} }
}

==============================Original Headers==============================
6, 35 -- From cljohnson33-at-gmail.com Tue Jan 27 17:23:42 2009
6, 35 -- Received: from yx-out-1718.google.com (yx-out-1718.google.com [74.125.44.153])
6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RNNfZJ010259
6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 17:23:42 -0600
6, 35 -- Received: by yx-out-1718.google.com with SMTP id 36so2657613yxh.0
6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:23:41 -0800 (PST)
6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 35 -- d=gmail.com; s=gamma;
6, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
6, 35 -- :date:message-id:subject:from:to:content-type
6, 35 -- :content-transfer-encoding;
6, 35 -- bh=6VxQLoCgeWnQS8yLUFCYyTw7jp0qu6v+g0cCcnOLvA0=;
6, 35 -- b=k9rnm3IgtR60LMtKTyTljOQHdKV3K0xJsBwn0NswiGFfOS6+fN500bewkQpY3AWWGK
6, 35 -- +PZQIURVw8oS276GHrNyojShHZANz8w+T7VFUqZKcMKj0We9AyqWby1DcI7abGmA2FOo
6, 35 -- z3+TcrZFON497Alyh8UyAnN/xb1DG3Dp4e0g0=
6, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
6, 35 -- d=gmail.com; s=gamma;
6, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
6, 35 -- :content-type:content-transfer-encoding;
6, 35 -- b=npdd4MHtTjbBlBHfN/GbYFG2AvOLID1KX44HgAsWSh0me/wy7fT4OOiUSOZxiM2G+1
6, 35 -- uCWLbxFS1e7mPq+CuhNOEMmU4Y3uf3fTe7Ii56sKmHKO8OP13Jx5DEJ4ZwPIwn2aNy2x
6, 35 -- y9WzcFo5mGsmWWnui1GPLx7+nLfse8VKvUaNQ=
6, 35 -- MIME-Version: 1.0
6, 35 -- Received: by 10.150.11.14 with SMTP id 14mr347575ybk.101.1233098621512; Tue,
6, 35 -- 27 Jan 2009 15:23:41 -0800 (PST)
6, 35 -- In-Reply-To: {611c9b6f0901271522o7998ab43p2aed2e4e16cd429a-at-mail.gmail.com}
6, 35 -- References: {200901272231.n0RMVQkI024095-at-ns.microscopy.com}
6, 35 -- {611c9b6f0901271522o7998ab43p2aed2e4e16cd429a-at-mail.gmail.com}
6, 35 -- Date: Tue, 27 Jan 2009 18:23:41 -0500
6, 35 -- Message-ID: {611c9b6f0901271523t5b603428iad45ff9f8cc0e361-at-mail.gmail.com}
6, 35 -- Subject: Re: [Microscopy] Re: viaWWW: preparation of plan-view TEM samples
6, 35 -- From: Craig Johnson {cljohnson33-at-gmail.com}
6, 35 -- To: microscopy-at-microscopy.com
6, 35 -- Content-Type: text/plain; charset=ISO-8859-1
6, 35 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: contact-at-integrityscientific.com
Date: Wed, 28 Jan 2009 03:47:08 -0600
Subject: [Microscopy] Re: viaWWW: preparation of plan-view TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just to add to Ron Anderson's and Craig Johnson's posts - if you use
glycopthalate wax (which is often sold under a trade name of QuickStick
or something like that), you can put a small amount into a few ml of
acetone and paint it on the surface you want to protect. The wax is
totally soluble in acetone and should leave no residue (okay, I guess a
small amount of amorphous carbon on the atomic scale). I'm not sure
that nail varnish would do the same.

Richard Beanland

y.han-at-sheffield.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: y.han-at-sheffield.ac.uk
} Name: Yisong Han
}
} Organization: University of Sheffield
}
} Title-Subject: [Filtered] preparation of plan-view TEM samples
}
} Question: Dear All,
}
} Does anybody know how to protect plan-view TEM samples, which are
} thinned from one side only, from contamination during ion beam
} milling? Thanks very much in advance.
}
} Yisong
}
} Login Host: 143.167.204.169
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216
} 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]}
} 8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}


==============================Original Headers==============================
4, 29 -- From contact-at-integrityscientific.com Wed Jan 28 03:47:07 2009
4, 29 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0S9l7Y4015263
4, 29 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 03:47:07 -0600
4, 29 -- Received: from localhost (localhost [127.0.0.1])
4, 29 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n0S9l48e017243;
4, 29 -- Wed, 28 Jan 2009 09:47:04 GMT
4, 29 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk
4, 29 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1])
4, 29 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024)
4, 29 -- with LMTP id Y+Lij45W7ERS; Wed, 28 Jan 2009 09:46:59 +0000 (GMT)
4, 29 -- Received: from [137.205.164.101] (hosts-137-205-164-101 [137.205.164.101])
4, 29 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n0S9kies017068;
4, 29 -- Wed, 28 Jan 2009 09:46:44 GMT
4, 29 -- X-Envelope-From: contact-at-integrityscientific.com
4, 29 -- Message-ID: {4980297E.2010504-at-integrityscientific.com}
4, 29 -- Date: Wed, 28 Jan 2009 09:46:38 +0000
4, 29 -- From: Richard Beanland {contact-at-integrityscientific.com}
4, 29 -- Reply-To: contact-at-integrityscientific.com
4, 29 -- Organization: Integrity Scientific Ltd
4, 29 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
4, 29 -- MIME-Version: 1.0
4, 29 -- To: y.han-at-sheffield.ac.uk, microscopy-at-microscopy.com
4, 29 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples
4, 29 -- References: {200901272159.n0RLxEV4005110-at-ns.microscopy.com}
4, 29 -- In-Reply-To: {200901272159.n0RLxEV4005110-at-ns.microscopy.com}
4, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 29 -- Content-Transfer-Encoding: 7bit
4, 29 -- X-DCC-Warwick-Metrics: bluebell; whitelist
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 28 Jan 2009 06:44:33 -0600
Subject: [Microscopy] Re: viaWWW: Looking for carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

You wrote "The sample is non conducting and are sputtered with gold. ".
Depending of how thick and how grainy your gold coating is, the C signal
you see will only partially reflect the C present in the sample.
Absorbtion of the carbon line by the gold layer may be strong. A little
peak could reflect a low surface contamiantion as welle as a high carbon
presence in the sample.

An other way to test your sample, would be to do EDS at low voltage
(less then 5 kev, better 3 keV) without coating. It is possible (more or
less easy) to find conditions where one have no or low charging, without
coating. At low energy, one have too a much better emission yield on low
energy x-ray lines.

If contamination occurs, you should see it well at low voltage too, and
better with an in lens SE detector (of coarse not on the JXA).

As other have said, low energy lines are difficulte, C and N in
particular (but Be or B too !).

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



emer.ryan-at-dit.ie a écrit :
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: emer.ryan-at-dit.ie
} Name: Emer Ryan
}
} Organization: CREST DIT DUBLIN
}
} Title-Subject: [Filtered] Looking for carbon
}
} Question: Hello all,
}
} I have been given a sample to analyse. The sample is non conducting
} and are sputtered with gold. I've run an EDX and found various
} elements,nothing too interesting, but the spectrum includes carbon. I
} have explained to the requester that although carbon is present, I
} cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is
} depositing carbon during analysis. I note that folk are growing
} carbon whiskers on AFM tips to produce sharper tips but putting the
} tip in a SEM for a few minutes; the older the SEM, the better i.e.
} the SEM deposits carbon.....
} What is the general opinion regarding detecting carbon using EDX?
} Appreciated,
} Emer.
}
}
}
}
} Login Host: 147.252.66.48
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 2009
} 10, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
} 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QDvfm8009249
} 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 07:57:42 -0600
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]}
} 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600
} 10, 11 -- To: microscopy-at-microscopy.com
} 10, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver)
} 10, 11 -- Subject: viaWWW: Looking for carbon
} 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
11, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Jan 28 06:44:33 2009
11, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156])
11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SCiWJd002805
11, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 06:44:33 -0600
11, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
11, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n0SCiRAt016098
11, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 13:44:27 +0100 (CET)
11, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
11, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
11, 32 -- (No client certificate requested)
11, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 378263EC005
11, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 28 Jan 2009 13:44:15 +0100 (CET)
11, 32 -- Message-ID: {4980531E.4010005-at-ipcms.u-strasbg.fr}
11, 32 -- Date: Wed, 28 Jan 2009 13:44:14 +0100
11, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
11, 32 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
11, 32 -- MIME-Version: 1.0
11, 32 -- To: Microscopy-at-microscopy.com
11, 32 -- Subject: Re: [Microscopy] viaWWW: Looking for carbon
11, 32 -- References: {200901261404.n0QE4e50017078-at-ns.microscopy.com}
11, 32 -- In-Reply-To: {200901261404.n0QE4e50017078-at-ns.microscopy.com}
11, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 32 -- Content-Transfer-Encoding: 8bit
11, 32 -- X-IPCMS-MailScanner: Found to be clean
11, 32 -- X-IPCMS-MailScanner-SpamScore: s
11, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
11, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.156]); Wed, 28 Jan 2009 13:44:27 +0100 (CET)
11, 32 -- X-Virus-Scanned: ClamAV 0.94.2/8914/Wed Jan 28 07:40:00 2009 on mr6.u-strasbg.fr
11, 32 -- X-Virus-Status: Clean
11, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
11, 32 -- autolearn=disabled version=3.2.5
11, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr6.u-strasbg.fr
==============================End of - Headers==============================




From: gary-at-perfendo.com
Date: Wed, 28 Jan 2009 07:32:09 -0600
Subject: [Microscopy] viaWWW: book request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gary-at-perfendo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gary-at-perfendo.com
Name: Gary B. Carr

Organization: Pacific Endodontic Research Foundation

Title-Subject: [Filtered] book request

Question: Does anyone have a copy of: Biomedical Electron Microscopy:
Illustrated Methods and Interpretations by Maunsbach
that they would like to sell me?

858-558-3636

Thanks in advance,
Gary


Login Host: 74.7.224.162
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 07:32:08 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SDW7x7017867
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 07:32:08 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240803c5a60ebf4c3c-at-[206.69.208.22]}
9, 11 -- Date: Wed, 28 Jan 2009 07:32:07 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: gary-at-perfendo.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: book request
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jrcastel-at-quim.ucm.es
Date: Wed, 28 Jan 2009 07:32:32 -0600
Subject: [Microscopy] viaWWW: Sample sensitivity under de e-beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jrcastel-at-quim.ucm.es as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jrcastel-at-quim.ucm.es
Name: Julio Ramirez-Castellanos

Organization: Complutense Univ. Madrid

Title-Subject: [Filtered] Sample sensitivity under de e-beam

Question: I am trying to examine a GeO2 sample by HRTEM, however, the
sample is very unstable under de electron beam .... what can I do
.... thanks.

Login Host: 147.96.6.115
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 07:32:32 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SDWVmt018357
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 07:32:31 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c5a60edd532c-at-[206.69.208.22]}
6, 11 -- Date: Wed, 28 Jan 2009 07:32:30 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: jrcastel-at-quim.ucm.es (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Sample sensitivity under de e-beam
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 28 Jan 2009 09:42:28 -0600
Subject: [Microscopy] Demise of Spurr's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There was a recent string on the listserv concerning substitutes for the
late, lamented Spurr's resin. Really? Is Spurr's gone? We just
ordered some from our usual suppliers and I haven't heard anything about
its being discontinued. Have I missed something (not impossible or
even unlikely some days)?

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
6, 27 -- From TindallR-at-missouri.edu Wed Jan 28 09:42:27 2009
6, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SFgRA9017634
6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 09:42:27 -0600
6, 27 -- X-IronPort-Anti-Spam-Filtered: true
6, 27 -- X-IronPort-Anti-Spam-Result: ApoEANQLgEnRauUo/2dsb2JhbAC+SgEJhTyIR4QagS0G
6, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
6, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 28 Jan 2009 09:42:27 -0600
6, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Wed, 28 Jan 2009 09:42:27 -0600
6, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5
6, 27 -- Content-class: urn:content-classes:message
6, 27 -- MIME-Version: 1.0
6, 27 -- Content-Type: text/plain;
6, 27 -- charset="us-ascii"
6, 27 -- Subject: Demise of Spurr's?
6, 27 -- Date: Wed, 28 Jan 2009 09:42:26 -0600
6, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D3D-at-UM-XMAIL08.um.umsystem.edu}
6, 27 -- X-MS-Has-Attach:
6, 27 -- X-MS-TNEF-Correlator:
6, 27 -- Thread-Topic: Demise of Spurr's?
6, 27 -- Thread-Index: AcmBXwTN8cOZv1rDTk25PFQ4yTYXJg==
6, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
6, 27 -- To: {microscopy-at-microscopy.com}
6, 27 -- X-OriginalArrivalTime: 28 Jan 2009 15:42:27.0274 (UTC) FILETIME=[059BC2A0:01C9815F]
6, 27 -- Content-Transfer-Encoding: 8bit
6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SFgRA9017634
==============================End of - Headers==============================




From: Matt.Laframboise-at-Kraft.com
Date: Wed, 28 Jan 2009 10:06:33 -0600
Subject: [Microscopy] Job Opportunity: Scientist/Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scientist/Microscopist (# 932058)

Kraft Foods, Global, Inc. is currently seeking a Scientist/Microscopist
to work in our R&D facility in Glenview,IL.

The candidate will thrive on the opportunity to resolve problems/build
knowledge in the Food R&D arena by the application of advanced
microscopy. The candidate will work in a team with other structural
scientists (light microscopy, Confocal, SEM,TEM) and chemical and
physical scientists.

Utilize expertise in spatial resolution ranging from light microscopy to
electron microscopy combined with spectral imaging in order to resolve
problems and build knowledge in Food/Packaging. PhD or equivalent in
biology/biochemicstry with a proficiency in advanced microscopy
techniques including Energy Filtering TEM, SEM x-ray microanalysis, and
histochemistry are expected along with both solo and team work
capablities. The position requires the ability to work on several
projects simultaneously and communicate finding to other scientists,
product developers and managers.

PhD in Bio/Chem or Equivalent
Minimum 1-3 years of experience in Biology/Life Sciences
Minimum 1-3 years of experience in Chemistry
High skill level in EFTEM & SEM
Experience in EM sample preparation methods for diverse foods
Computer skills in image analysis and digital imaging
Food Sciences

KRAFT Foods is the World's second largest food and Beverage Company. For
more than 100 years we have been dedicated to helping people around the
world eat and live better. Hundreds of millions of times a day in over
150 countries consumers reach for their favorite Kraft Brands. Kraft has
approximately 98,000 employees in 68 countries.

Diversity generates new ideas that yield the innovation we need for
company growth, competitive advantage, and industry leadership. KRAFT
strives to create a rich and stimulating work climate to help you
develop your skills and advance your career- team environments designed
to promote and reward individuality, innovation, leadership, and strong
business results based on a solid understanding of the global
organization.

Kraft Foods offers a competitive compensation and benefits package
including health care coverage, generous 401k match, annual incentive
bonus and paid time off.

Candidates who are interested in applying for this position should do so
via the Kraft Foods applicant tracking system, by cutting and pasting
the following link into their web browser:

Matt LaFramboise
Kraft Foods
R&D Recruiter
847.646.9241
Matt.laframboise-at-kraft.com


==============================Original Headers==============================
11, 26 -- From Matt.Laframboise-at-Kraft.com Wed Jan 28 10:06:33 2009
11, 26 -- Received: from mail5.kraft.com (mail5.kraft.com [206.228.222.60])
11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SG6VPE032117
11, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:06:32 -0600
11, 26 -- X-IronPort-AV: E=McAfee;i="5300,2777,5508"; a="95208484"
11, 26 -- Received: from unknown (HELO kftusoktulxbh03.KRFT.Net) ([10.53.184.16])
11, 26 -- by mail5.kraft.com with ESMTP; 28 Jan 2009 11:02:36 -0500
11, 26 -- Received: from KFTUSPAWBAXMB31.KRFT.Net ([10.53.184.64]) by kftusoktulxbh03.KRFT.Net with Microsoft SMTPSVC(6.0.3790.3959);
11, 26 -- Wed, 28 Jan 2009 10:02:36 -0600
11, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 26 -- Content-class: urn:content-classes:message
11, 26 -- MIME-Version: 1.0
11, 26 -- Content-Type: text/plain;
11, 26 -- charset="us-ascii"
11, 26 -- Subject: Job Opportunity: Scientist/Microscopist
11, 26 -- Date: Wed, 28 Jan 2009 11:02:35 -0500
11, 26 -- Message-ID: {0A7A37452676694E97DBE0232CF6FA3A4DE425-at-KFTUSPAWBAXMB31.KRFT.Net}
11, 26 -- X-MS-Has-Attach:
11, 26 -- X-MS-TNEF-Correlator:
11, 26 -- Thread-Topic: Job Opportunity: Scientist/Microscopist
11, 26 -- Thread-Index: AcmBYdWz/H5+Ay93SNm8OLAmrxTSVA==
11, 26 -- From: {Matt.Laframboise-at-Kraft.com}
11, 26 -- To: {Microscopy-at-microscopy.com}
11, 26 -- X-OriginalArrivalTime: 28 Jan 2009 16:02:36.0271 (UTC) FILETIME=[D63A0FF0:01C98161]
11, 26 -- Content-Transfer-Encoding: 8bit
11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SG6VPE032117
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Wed, 28 Jan 2009 10:45:12 -0600
Subject: [Microscopy] Re: Demise of Spurr's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In accordance with "List" protocol, I am copying this to the List. I
sent it to Randy offline with some attachments that can't go to the
list. The essential attachment, the new formulation is included at the
bottom of this message.

Dale
-----------------------

Hi Randy,

I think I was responsible for starting the most recent thread on Spurr's
resin. At the time I had bought a Spurr's Kit from EMS and had problems
with it. I noticed that one of the components had been changed and was
far more viscous than the original component. I checked the included
docs and it gave the same mixture as the original and made no mention of
the viscosity - that was from the bottle.
------------------------------------------------
Note that one of the components of the Spurr's resin (VCD = ERL-4206) is
no longer made and is replaced by a new component (ERL-4221) that is
much more viscous and has a different WPE value. E. Ann Ellis has
published new formulations to use with the new resin components.
---------------------------------------------------
Several people told me of Ann Ellis' work/publications on this, and the
corrected formulation. See attached documents.
----------------------------
Corrected Formulation for Spurr Low Viscosity Embedding Medium Using the
Replacement Epoxide ERL 4221. E. Ann Ellis
Microsc Microanal 12(Supp 2), 2006
Microscopy and Imaging Center, BSBW 119/MS 2257, Texas A&M University,
College Station,
TX 77843-2257
-------------------------------
E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem
of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low
Viscosity Embedding Formulations"
---------------------------------------------

Eventually Stacie at EMS chimed in and said they had been aware of it
and she produced some data.. that had not been present in the catalog,
nor included with the kits they sold as Spurr's resin.....

Hope this helps.

Dale

------------------------------------------------
Spurr-replacement "New Formulation"

E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem
of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in
Spurr Low Viscosity Embedding Formulations"



All formulas are for a "10g batch" - with reference to (epoxy + acid
anhydride)

Epoxy Anhyride Catalyst Comments
-------- -------- --------- -------- ------

ERL 4221 NSA DER-736 DMAE weights are in grams

4.10 5.90 0.95 0.10 Hard
4.10 5.90 1.43 0.10 Standard
4.10 5.90 1.90 0.10 Soft


Low Viscosity Formula (Half the viscosity of the "Refomulated Spurr's")
-------------------------
ERL 4221 2.22g
Quetol-651 1.40g
NSA 6.38g
DER-736 1.43g
BDMA 0.2g
Some think BDMA should be less, 0.1g

I (DAC) used 0.09g DMAE (5 drops from a Samco #241 pipet) and it set
nicely in 16h -at- 70C




TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} There was a recent string on the listserv concerning substitutes for the
} late, lamented Spurr's resin. Really? Is Spurr's gone? We just
} ordered some from our usual suppliers and I haven't heard anything about
} its being discontinued. Have I missed something (not impossible or
} even unlikely some days)?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original Headers==============================
} 6, 27 -- From TindallR-at-missouri.edu Wed Jan 28 09:42:27 2009
} 6, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SFgRA9017634
} 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 09:42:27 -0600
} 6, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 6, 27 -- X-IronPort-Anti-Spam-Result: ApoEANQLgEnRauUo/2dsb2JhbAC+SgEJhTyIR4QagS0G
} 6, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
} 6, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 28 Jan 2009 09:42:27 -0600
} 6, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 6, 27 -- Wed, 28 Jan 2009 09:42:27 -0600
} 6, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5
} 6, 27 -- Content-class: urn:content-classes:message
} 6, 27 -- MIME-Version: 1.0
} 6, 27 -- Content-Type: text/plain;
} 6, 27 -- charset="us-ascii"
} 6, 27 -- Subject: Demise of Spurr's?
} 6, 27 -- Date: Wed, 28 Jan 2009 09:42:26 -0600
} 6, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D3D-at-UM-XMAIL08.um.umsystem.edu}
} 6, 27 -- X-MS-Has-Attach:
} 6, 27 -- X-MS-TNEF-Correlator:
} 6, 27 -- Thread-Topic: Demise of Spurr's?
} 6, 27 -- Thread-Index: AcmBXwTN8cOZv1rDTk25PFQ4yTYXJg==
} 6, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 6, 27 -- To: {microscopy-at-microscopy.com}
} 6, 27 -- X-OriginalArrivalTime: 28 Jan 2009 15:42:27.0274 (UTC) FILETIME=[059BC2A0:01C9815F]
} 6, 27 -- Content-Transfer-Encoding: 8bit
} 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SFgRA9017634
} ==============================End of - Headers==============================


==============================Original Headers==============================
20, 20 -- From dac-at-research.umass.edu Wed Jan 28 10:45:12 2009
20, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SGjABR015008
20, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:45:11 -0600
20, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged))
20, 20 -- (authenticated bits=0)
20, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SGj8Gh003537
20, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
20, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 11:45:08 -0500
20, 20 -- Message-ID: {49808BD1.20005-at-research.umass.edu}
20, 20 -- Date: Wed, 28 Jan 2009 11:46:09 -0500
20, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
20, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
20, 20 -- MIME-Version: 1.0
20, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
20, 20 -- Subject: Re: [Microscopy] Demise of Spurr's?
20, 20 -- References: {200901281548.n0SFmUpb025875-at-ns.microscopy.com}
20, 20 -- In-Reply-To: {200901281548.n0SFmUpb025875-at-ns.microscopy.com}
20, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
20, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Wed, 28 Jan 2009 10:48:56 -0600
Subject: [Microscopy] Re: Demise of Spurr's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

--On 28 January 2009 09:53 -0600 TindallR-at-missouri.edu wrote:
} -------------------------------------------------------------------------
} --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------

} There was a recent string on the listserv concerning substitutes for the
} late, lamented Spurr's resin. Really? Is Spurr's gone? We just
} ordered some from our usual suppliers and I haven't heard anything about
} its being discontinued. Have I missed something (not impossible or
} even unlikely some days)?
}
} Cheers,
} Randy

Hi Randy,
I'd be immensely grateful if you can provide me with a source. It's
not available (it being the VCD - vinylcyclohexene dioxide - component of
the mixture) here in the UK for sure, and the low viscosity resin
substitutes I've tried are nowhere near as good for ease of sectioning and
staining compared with Spurr resin.
I'm almost tempted to ask you to let me know the source
individually and not via the server, because I reckon the stocks will
disappear rapidly....but I won't be that mean!
Regards,
Jules

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG

==============================Original Headers==============================
4, 24 -- From bafg3-at-sussex.ac.uk Wed Jan 28 10:48:56 2009
4, 24 -- Received: from karpinski.uscs.susx.ac.uk (karpinski.uscs.susx.ac.uk [139.184.14.85])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SGmtnW017822
4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:48:55 -0600
4, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:4865)
4, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64)
4, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
4, 24 -- id KE6XIJ-000JRA-B2
4, 24 -- for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 16:51:55 +0000
4, 24 -- Date: Wed, 28 Jan 2009 16:48:53 -0000
4, 24 -- To: Microscopy-at-microscopy.com
4, 24 -- Subject: Re: [Microscopy] Demise of Spurr's?
4, 24 -- Message-ID: {505719039.1233161332-at-ls0130.lifesci.susx.ac.uk}
4, 24 -- In-Reply-To: {200901281553.n0SFr91A031204-at-ns.microscopy.com}
4, 24 -- Originator-Info: login-token=Mulberry:01jMl4vu4q3iXACdgY9S26PO0xAC7j5e0kuj7j;
4, 24 -- token_authority=postmaster-at-central.susx.ac.uk
4, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
4, 24 -- Content-Transfer-Encoding: 7bit
4, 24 -- Content-Disposition: inline
4, 24 -- X-Sussex: true
4, 24 -- X-Sussex-transport: remote_smtp_rew
4, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Wed, 28 Jan 2009 10:58:09 -0600
Subject: [Microscopy] Demise of Spurr's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,
Yes, I'd love the attachments if they give the corrected formula
for the new resin mix. I did try it before and it wasn't good for me!
Appreciate your (and Dale's) help,
Jules

--On 28 January 2009 10:52 -0600 "Tindall, Randy D."
{TindallR-at-missouri.edu} wrote:

} Hi Jules,
}
} Hopefully the Dale Callahan post clears this up. I think we get the
} same formulation as you, but they still call it "Spurr's". I can send
} you Dale's attachments, if you like.
}
} Good luck,
} Randy
}
} -----Original Message-----
} From: j.r.thorpe-at-sussex.ac.uk [mailto:j.r.thorpe-at-sussex.ac.uk]
} Sent: Wednesday, January 28, 2009 10:50 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Re: Demise of Spurr's?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} --On 28 January 2009 09:53 -0600 TindallR-at-missouri.edu wrote:
} }
} ------------------------------------------------------------------------
} -
} } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ------------------------------------------------------------------------
} -
}
} } There was a recent string on the listserv concerning substitutes for
} the
} } late, lamented Spurr's resin. Really? Is Spurr's gone? We just
} } ordered some from our usual suppliers and I haven't heard anything
} about
} } its being discontinued. Have I missed something (not impossible or
} } even unlikely some days)?
} }
} } Cheers,
} } Randy
}
} Hi Randy,
} I'd be immensely grateful if you can provide me with a source.
} It's
} not available (it being the VCD - vinylcyclohexene dioxide - component
} of
} the mixture) here in the UK for sure, and the low viscosity resin
} substitutes I've tried are nowhere near as good for ease of sectioning
} and
} staining compared with Spurr resin.
} I'm almost tempted to ask you to let me know the source
} individually and not via the server, because I reckon the stocks will
} disappear rapidly....but I won't be that mean!
} Regards,
} Jules
}
} Dr. Julian R. Thorpe
} (Office 2C9/Lab 2C11-13)
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
}
} ==============================Original
} Headers==============================
} 4, 24 -- From bafg3-at-sussex.ac.uk Wed Jan 28 10:48:56 2009
} 4, 24 -- Received: from karpinski.uscs.susx.ac.uk
} (karpinski.uscs.susx.ac.uk [139.184.14.85])
} 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n0SGmtnW017822
} 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009
} 10:48:55 -0600
} 4, 24 -- Received: from ls0130.lifesci.susx.ac.uk
} ([139.184.162.69]:4865)
} 4, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64)
} 4, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
} 4, 24 -- id KE6XIJ-000JRA-B2
} 4, 24 -- for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 16:51:55
} +0000
} 4, 24 -- Date: Wed, 28 Jan 2009 16:48:53 -0000
} 4, 24 -- To: Microscopy-at-microscopy.com
} 4, 24 -- Subject: Re: [Microscopy] Demise of Spurr's?
} 4, 24 -- Message-ID: {505719039.1233161332-at-ls0130.lifesci.susx.ac.uk}
} 4, 24 -- In-Reply-To: {200901281553.n0SFr91A031204-at-ns.microscopy.com}
} 4, 24 -- Originator-Info:
} login-token=Mulberry:01jMl4vu4q3iXACdgY9S26PO0xAC7j5e0kuj7j;
} 4, 24 -- token_authority=postmaster-at-central.susx.ac.uk
} 4, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
} 4, 24 -- MIME-Version: 1.0
} 4, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
} 4, 24 -- Content-Transfer-Encoding: 7bit
} 4, 24 -- Content-Disposition: inline
} 4, 24 -- X-Sussex: true
} 4, 24 -- X-Sussex-transport: remote_smtp_rew
} 4, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
} ==============================End of -
} Headers==============================



Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
7, 24 -- From bafg3-at-sussex.ac.uk Wed Jan 28 10:58:09 2009
7, 24 -- Received: from karpinski.uscs.susx.ac.uk (karpinski.uscs.susx.ac.uk [139.184.14.85])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SGw8h3001318
7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:58:08 -0600
7, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:4948)
7, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64)
7, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
7, 24 -- id KE6XXW-00014C-H1
7, 24 -- for microscopy-at-microscopy.com; Wed, 28 Jan 2009 17:01:08 +0000
7, 24 -- Date: Wed, 28 Jan 2009 16:58:06 -0000
7, 24 -- To: microscopy-at-microscopy.com
7, 24 -- Subject: RE: [Microscopy] Re: Demise of Spurr's?
7, 24 -- Message-ID: {506272204.1233161886-at-ls0130.lifesci.susx.ac.uk}
7, 24 -- In-Reply-To: {91108EF9255B394CBF8B7E3789814A4103CD7D47-at-UM-XMAIL08.um.umsystem.edu}
7, 24 -- Originator-Info: login-token=Mulberry:01y7rY0y0bPjr/+jgtNB75LU1GPx9D+iwV+S9D;
7, 24 -- token_authority=postmaster-at-central.susx.ac.uk
7, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
7, 24 -- MIME-Version: 1.0
7, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
7, 24 -- Content-Transfer-Encoding: 7bit
7, 24 -- Content-Disposition: inline
7, 24 -- X-Sussex: true
7, 24 -- X-Sussex-transport: remote_smtp_rew
7, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Wed, 28 Jan 2009 12:45:41 -0600
Subject: [Microscopy] Re: Demise of Spurr's?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is a link to the information you are looking for on Ann's work:

http://www.laddresearch.com/kitinstr/spurrkitNewformula.pdf

Dr. Charles Duvic
Chief Chemist

Disclaimer: Ladd Research is a supply house for microscopy supplies
including Spurr's Kits.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com



At 12:04 PM 1/28/2009, j.r.thorpe-at-sussex.ac.uk wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
16, 27 -- From jd-at-laddresearch.com Wed Jan 28 12:45:41 2009
16, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29])
16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SIjfdG028389
16, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:45:41 -0600
16, 27 -- Received: from 1LSFPu-0001wV-WC by bean.electric.net with emc1-ok (Exim 4.69)
16, 27 -- (envelope-from {jd-at-laddresearch.com} )
16, 27 -- id 1LSFPv-0001y1-Uh; Wed, 28 Jan 2009 10:45:39 -0800
16, 27 -- Received: by emcmailer; Wed, 28 Jan 2009 10:45:39 -0800
16, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
16, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256)
16, 27 -- (Exim 4.69)
16, 27 -- (envelope-from {jd-at-laddresearch.com} )
16, 27 -- id 1LSFPu-0001wV-WC; Wed, 28 Jan 2009 10:45:39 -0800
16, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
16, 27 -- Date: Wed, 28 Jan 2009 13:45:29 -0500
16, 27 -- To: j.r.thorpe-at-sussex.ac.uk
16, 27 -- From: jd {jd-at-laddresearch.com}
16, 27 -- Subject: Re: [Microscopy] Demise of Spurr's?
16, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
16, 27 -- In-Reply-To: {200901281704.n0SH4QP2019055-at-ns.microscopy.com}
16, 27 -- References: {200901281704.n0SH4QP2019055-at-ns.microscopy.com}
16, 27 -- Mime-Version: 1.0
16, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
16, 27 -- X-Outbound-IP: 216.204.198.170
16, 27 -- X-Env-From: jd-at-laddresearch.com
16, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
16, 27 -- Message-Id: {E1LSFPv-0001y1-Uh-at-bean.electric.net}
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 28 Jan 2009 13:02:10 -0600
Subject: [Microscopy] Pre-embedding staining of embedding media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

I have a question regarding "pre-embedding staining" of 812 or Spurr's
resin.

TEM is a useful tool for study of interaction of dental adhesives with
dentin (which is mostly mineral). Unfortunately for me embedding resin
and dental adhesive resin have about the same electron density, making
it difficult, if not impossible, to tell them apart. It makes difficult
to observe depth of infiltration, porosity, etc. So far for studies like
these I have cut sections from not embedded teeth with mixed results.

I would greatly appreciate any suggestions about changing electron
density of embedding media.

Many thanks in advance,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
10, 23 -- From DusevichV-at-umkc.edu Wed Jan 28 13:02:10 2009
10, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SJ29Aq010249
10, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 13:02:10 -0600
10, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 23 -- Wed, 28 Jan 2009 13:02:09 -0600
10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 23 -- Content-class: urn:content-classes:message
10, 23 -- MIME-Version: 1.0
10, 23 -- Content-Type: text/plain;
10, 23 -- charset="us-ascii"
10, 23 -- Subject: Pre-embedding staining of embedding media
10, 23 -- Date: Wed, 28 Jan 2009 13:02:08 -0600
10, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7C1-at-KC-MSX1.kc.umkc.edu}
10, 23 -- X-MS-Has-Attach:
10, 23 -- X-MS-TNEF-Correlator:
10, 23 -- Thread-Topic: Pre-embedding staining of embedding media
10, 23 -- thread-index: AcmBeusXCv65/7XGRuix7tM50ZGBBQ==
10, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
10, 23 -- To: {Microscopy-at-microscopy.com}
10, 23 -- X-OriginalArrivalTime: 28 Jan 2009 19:02:09.0137 (UTC) FILETIME=[EB5AF610:01C9817A]
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SJ29Aq010249
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Wed, 28 Jan 2009 14:30:27 -0600
Subject: [Microscopy] LKB Knifemaker 7800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Years ago (1980s), I followed someone else's lead to dope epoxy resin
with iodoform to increase its average atomic number.

I was studying minerals in coal and standard epoxy resins provided
practically no contrast with coal in backscattered electron images. We
ended up dissolving about 15 wt.% iodoform in epoxy resin. We later
added the hardener and the epoxy behaved in much the same way as the
original two-part epoxy. That is, hardness and polymerization were
similar.

The resulting epoxy offered significant contrast with coal and allowed
us to proceed with automated image analyses.

Be advised that iodoform is rather nasty and needs to be handled with
care.

For what it's worth, I began working with iodoform shortly after the
Right-to-know laws were passed. Suppliers had recently started including
MSDSs with all their chemicals. I was still bemused that my
chemical-grade calcium carbonate "should be disposed of in an approved,
chemical-waste landfill" when my iodoform arrived. I had to do some more
of my own research to determine if iodoform was really as nasty as the
MSDS said or not. (It is.) Someone was crying wolf about the calcium
carbonate while a contractor was laying tons of the stuff just outside
our building as a base for the parking lot.

Warren S.

-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: Wednesday, January 28, 2009 1:03 PM
To: wesaia-at-iastate.edu

Dear friends:

This is a message asking for help adjusting our LKB Knifemaker(s).

I am teaching an ultramicrotomy class to 20 community college students
and we use glass knives. My job is to make sure the instruments are
adjusted so they can make good knives.

We have 6, yes that's six, LKB Knifemakers in various states of
(dis)repair. I need to build at least one good one from all of these.

Most of them were donated, some are better than others, I can probably
figure out what to do, but just wanted to check with any experts on
the list.

In another life, I used an LKB Knifemaker that worked pretty well.
That one was serviced by an LKB tech once and a while and, as usual,
had all the adjustments taped down with notes saying things like 'Do
not adjust'. Since I could at least follow directions, I never tried
to change the adjustments.

Now in my new life, I have 6 things that look like Knifemakers sitting
on the bench and 20 sets of eyes looking at me expecting these things
to work perfectly every time. I have done the expected Googling and
found a few remarks about adjusting Knifemakers, but before I start
tinkering, I wanted to check with the list to see if someone has the
magic formula to insure success. Experience trumps google every time.

Thanks

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009
13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937
13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600
13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B
13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F
13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
13, 42 -- with ESMTP id 45280969 for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 12:30:23 -0800
13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
13, 42 -- ESMTP id KE76WG00.4MS for {Microscopy-at-microscopy.com} ; Wed, 28
13, 42 -- Jan 2009 12:14:40 -0800
13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 9F5C78F74D04
13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
13, 42 -- X-Virus-Scanned: amavisd-new at
13, 42 -- X-Spam-Flag: NO
13, 42 -- X-Spam-Score: -2.499
13, 42 -- X-Spam-Level:
13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
13, 42 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
13, 42 -- with ESMTP id R34HTgsbNAlK for {Microscopy-at-microscopy.com} ;
13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST)
13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74])
13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5
13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu}
13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
13, 42 -- To: Microscopy-at-microscopy.com
13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 42 -- Content-Transfer-Encoding: 7bit
13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 42 -- Subject: LKB Knifemaker 7800
13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800
13, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 28 Jan 2009 15:38:42 -0600
Subject: [Microscopy] Re: LKB Knifemaker 7800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon,

Another idea, especially since you have 6 of these miserable things.
Rebuild one or two to make knives by the balanced-break method. How
to do this is detailed by Herbert Hagler, Chap. 5, "Ultramicrotomy
for Biological Electron Microscopy" pp67-96 in
Electron MIcroscopy: Methods and Protocols, John Kuo, ed., 2nd
edition. Specifically, Note 1 pg 91 ff.
I only have one knife-maker and it works well enough to torment
students, so I haven't done this. If I had 2, I would've by now.

Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
5, 26 -- From oshel1pe-at-cmich.edu Wed Jan 28 15:38:42 2009
5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLcgmJ023273
5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:38:42 -0600
5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0SLccFS005101;
5, 26 -- Wed, 28 Jan 2009 16:38:38 -0500
5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 26 -- Wed, 28 Jan 2009 16:38:15 -0500
5, 26 -- Mime-Version: 1.0
5, 26 -- Message-Id: {f06240812c5a67f74468f-at-[141.209.160.249]}
5, 26 -- In-Reply-To: {200901282033.n0SKXsOq010961-at-ns.microscopy.com}
5, 26 -- References: {200901282033.n0SKXsOq010961-at-ns.microscopy.com}
5, 26 -- Date: Wed, 28 Jan 2009 16:38:12 -0500
5, 26 -- To: jkrupp-at-deltacollege.edu
5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 26 -- Subject: Re: [Microscopy] LKB Knifemaker 7800
5, 26 -- Cc: Microscopy-at-microscopy.com
5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 26 -- X-OriginalArrivalTime: 28 Jan 2009 21:38:15.0225 (UTC) FILETIME=[B9FA9290:01C98190]
5, 26 -- X-Canit-CHI2: 0.00
5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
5, 26 -- X-CanItPRO-Stream: default
5, 26 -- X-Canit-Stats-ID: 8097891 - 412a4c8ab3d3
5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Wed, 28 Jan 2009 15:44:31 -0600
Subject: [Microscopy] Re: LKB Knifemaker 7800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,

The magic formula is the manual. You didn't mention having one. It goes
over all the features of the knives and adjustments to get good ones. It
is really very simple once you get into it - only about 4 or 5 things to
tweek.

These things are fairly rugged. There is a trick to getting the movable
overhead clamping part off; You position the clamping lever at ~45o to
the front (or back??) and lift and it slides up and off. You may need to
clean the mating faces well with solvent to remove the sticky grease you
may find there. These parts are brass and only need to be clean. The
scoring part that you pull out is the same (clean) but I do use some
silicone lube lightly on it; never anything that would get gummy, and
some very light grease on the cam that the scoring wheel follows (inside
the silver outer tube; again here start with it clean, and use sparingly
a light grease only on the cam face, not the scoring wheel.

Note that the score wheel can be set for cutting squares from strips
(the parallel lines) or scoring squares for knives - the square with a
line symbol; these settings determine where the scoring wheel drops onto
the glass and where it lifts off, very important.

The twiddle knobs at the top and bottom of the glass-position clamping
forks position the glass square so that the { {fixed} } score falls on it
just so. At the top and bottom there are 2 adjustments one is side-side
and one clamps the metal fork that contacts the knife corner to set the
position of the glass for and aft; the front one sets the position and
the rear fork only applies pressure. You can change the angle of the
score relative to the corners, and control where the score lifts off the
square at the near corner by adjusting the clamping forks side-to-side
and fore and aft; the side-side can be done at both top and bottom to
accentuate your frustration but eventually place the score in the
correct position. Adjustment for a score to a position very close to the
near corner is critical for a controlled break; the break should come
out on the right-hand side of the near corner and within 0.5mm of the
corner - and this will be the cutting edge of the good knife. In good
adjustment you will get 2 knives that are fairly symmetrical but not
completely. The other opposite cutting edge will be less controlled and
that knife is rarely as good, but usually just fine for facing up blocks
or semi-thins.

Scoring wheel must be sharp. Easily replaced. I have a source for them
if you need new ones. They are a standard size still available. Google
works too.

Also the scoring pressure must be light - thus the need for the sharp
wheel; too much pressure, too deep and the score is broad and knives
will not break smoothly and be erratic in shape. A gentle breaking
pressure and slow break is preferred for the best knives.

And all glass is not created equal. If you don't have a stock see if
someone can make a recommendation. I have a lot of old LKB glass and it
is still very good. People have brought newer glass and some of it does
not break so well.

Let me know if you need a copy of the instructions w/pictures - the
originals with drawings of the score marks, angles, etc. are very helpful.


Dale

jkrupp-at-deltacollege.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear friends:
}
} This is a message asking for help adjusting our LKB Knifemaker(s).
}
} I am teaching an ultramicrotomy class to 20 community college students
} and we use glass knives. My job is to make sure the instruments are
} adjusted so they can make good knives.
}
} We have 6, yes that's six, LKB Knifemakers in various states of
} (dis)repair. I need to build at least one good one from all of these.
}
} Most of them were donated, some are better than others, I can probably
} figure out what to do, but just wanted to check with any experts on
} the list.
}
} In another life, I used an LKB Knifemaker that worked pretty well.
} That one was serviced by an LKB tech once and a while and, as usual,
} had all the adjustments taped down with notes saying things like 'Do
} not adjust'. Since I could at least follow directions, I never tried
} to change the adjustments.
}
} Now in my new life, I have 6 things that look like Knifemakers sitting
} on the bench and 20 sets of eyes looking at me expecting these things
} to work perfectly every time. I have done the expected Googling and
} found a few remarks about adjusting Knifemakers, but before I start
} tinkering, I wanted to check with the list to see if someone has the
} magic formula to insure success. Experience trumps google every time.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original Headers==============================
} 13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009
} 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
} 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937
} 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600
} 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
} 13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B
} 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
} 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
} 13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F
} 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
} 13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} 13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 13, 42 -- with ESMTP id 45280969 for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 12:30:23 -0800
} 13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} 13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
} 13, 42 -- ESMTP id KE76WG00.4MS for {Microscopy-at-microscopy.com} ; Wed, 28
} 13, 42 -- Jan 2009 12:14:40 -0800
} 13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 9F5C78F74D04
} 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} 13, 42 -- X-Virus-Scanned: amavisd-new at
} 13, 42 -- X-Spam-Flag: NO
} 13, 42 -- X-Spam-Score: -2.499
} 13, 42 -- X-Spam-Level:
} 13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
} 13, 42 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
} 13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
} 13, 42 -- with ESMTP id R34HTgsbNAlK for {Microscopy-at-microscopy.com} ;
} 13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} 13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74])
} 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5
} 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} 13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu}
} 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} 13, 42 -- To: Microscopy-at-microscopy.com
} 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 13, 42 -- Content-Transfer-Encoding: 7bit
} 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 13, 42 -- Subject: LKB Knifemaker 7800
} 13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800
} 13, 42 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================

==============================Original Headers==============================
12, 20 -- From dac-at-research.umass.edu Wed Jan 28 15:44:31 2009
12, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLiUnn000740
12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:44:31 -0600
12, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged))
12, 20 -- (authenticated bits=0)
12, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SLiTJx031617
12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 16:44:30 -0500
12, 20 -- Message-ID: {4980D1FA.6080608-at-research.umass.edu}
12, 20 -- Date: Wed, 28 Jan 2009 16:45:30 -0500
12, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
12, 20 -- MIME-Version: 1.0
12, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
12, 20 -- Subject: Re: [Microscopy] LKB Knifemaker 7800
12, 20 -- References: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com}
12, 20 -- In-Reply-To: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com}
12, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
12, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: guosheng.liu-at-usask.ca
Date: Wed, 28 Jan 2009 18:22:49 -0600
Subject: [Microscopy] viaWWW: looking for "free" parts for Philips 505 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both guosheng.liu-at-usask.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: guosheng.liu-at-usask.ca
Name: Guosheng Liu

Organization: Biology Dept, Univ of Saskatchewan

Title-Subject: [Filtered] looking for "free" parts for Philips 505 SEM

Question: Dear All:

Our old Philips 505 SEM stopped working for a while and I still want
to re-energize it at a low cost if possible.

Due to no immediate budget available for ordering new ones, I am
wondering if there is a similar model SEM sitting around but we can
salvage some parts (see following) from it before it is disposed.
Detailed salvaging /shipping fees could be discussed later.

5322-271-34165 Micro-switch
5322-282-54055 Thermal switch 60C
5322-282-50036 Thermal switch 112C
5322-695-14541 PV10P Valve
5322-695-14542 Heating element
SCA module A2 board TV scan generator.

Thank you!

Guosheng

Login Host: 128.233.123.40
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:22:48 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0MmdU022194
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:22:48 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240800c5a6a72f0719-at-[206.69.208.22]}
11, 11 -- Date: Wed, 28 Jan 2009 18:22:47 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: guosheng.liu-at-usask.ca (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: looking for "free" parts for Philips 505 SEM
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sue.tyler-at-noaa.gov
Date: Wed, 28 Jan 2009 18:23:26 -0600
Subject: [Microscopy] viaWWW: Paragon stain TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sue.tyler-at-noaa.gov
Name: Sue Tyler

Organization: Cooperative Oxford Laboratory

Title-Subject: [Filtered] Paragon stain TEM

Question: I have checked the listserver for Paragon staining of epoxy
resin and found several very helpful comments. I tried using
Martin's procedure without success. I came across another comment
about etching the slides first... Does anyone have experience with
this?

I have since tried Richardson's stain and it just doesn't give enough
differentiation. I am staining coral tissues that have been
decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in
1% Osmium and embedded in Spurr's.

Hope you can help.
Sue

Login Host: 205.156.36.37
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:23:26 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0NPRl022677
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:23:26 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c5a6a764137a-at-[206.69.208.22]}
8, 11 -- Date: Wed, 28 Jan 2009 18:23:25 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Paragon stain TEM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 28 Jan 2009 22:01:19 -0600
Subject: [Microscopy] Re: viaWWW: Paragon stain TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Sue-

I can't find my (antique) notes on etching at the moment, but I can sort
of remember, and it might get you started while someone else comes up with
something. I was etching epoxy resins with saturated ethanolic NaOH to do
PAS.

Make a solution of saturated ethanolic NaOH by dumping a lot of NaOH
pellets in a bottle of absolute ethanol, like an inch and a half in a pint
bottle. Put it aside for a couple of weeks until it looks like cognac.
Soak slides in this solution in a coplin jar for (and this is where I
can't remember - Two hours? Two days?). Sections used to easily come off
those old, plain slides, so I was careful not to agitate. I think they
would stay on better with Superfrost Plus or treated slides. Go ahead and
start making your cognac .. er.. etching solution and I'll look for my
PAS protocol.

Aloha from chilly Hawaii (OK, so it's 67F)
Tina


} Organization: Cooperative Oxford Laboratory
}
} Title-Subject: [Filtered] Paragon stain TEM
}
} Question: I have checked the listserver for Paragon staining of epoxy
} resin and found several very helpful comments. I tried using
} Martin's procedure without success. I came across another comment
} about etching the slides first... Does anyone have experience with
} this?
}
} I have since tried Richardson's stain and it just doesn't give enough
} differentiation. I am staining coral tissues that have been
} decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in
} 1% Osmium and embedded in Spurr's.
}
} Hope you can help.
} Sue
}
} Login Host: 205.156.36.37
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:23:26 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0NPRl022677
} 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:23:26 -0600
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c5a6a764137a-at-[206.69.208.22]}
} 8, 11 -- Date: Wed, 28 Jan 2009 18:23:25 -0600
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Paragon stain TEM
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
8, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 28 22:01:19 2009
8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T41IS7022534
8, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 22:01:18 -0600
8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0T41Eox020137
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
8, 21 -- Wed, 28 Jan 2009 18:01:14 -1000 (HST)
8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0T41BjA020134;
8, 21 -- Wed, 28 Jan 2009 18:01:13 -1000 (HST)
8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
8, 21 -- Date: Wed, 28 Jan 2009 18:01:10 -1000 (HST)
8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 21 -- X-Sender: tina-at-halia
8, 21 -- To: sue.tyler-at-noaa.gov
8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 21 -- Subject: Re: [Microscopy] viaWWW: Paragon stain TEM
8, 21 -- In-Reply-To: {200901290024.n0T0OCU6025080-at-ns.microscopy.com}
8, 21 -- Message-ID: {Pine.GSO.4.21.0901281752000.20054-100000-at-halia}
8, 21 -- MIME-Version: 1.0
8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: smithj-at-winthrop.edu
Date: Thu, 29 Jan 2009 07:52:47 -0600
Subject: [Microscopy] viaWWW: etching epoxy sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I prefer KOH in MeOH. We use 5' etch, followed by 2 changes of absolute
MeOH. We published a protocol for Iron Hematoxylin/Eosin/Alcian blue
(which gives pretty differentiated staining on marine inverts) a while
back in Microskopie; I can send a .doc file to anyone interested.
If you use PAS after etching, watch for staining artifact--epoxy
embedding generates a bunch of 'em, at least with our invert material.
Julian

tina-at-pbrc.hawaii.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi, Sue-
}
} I can't find my (antique) notes on etching at the moment, but I can sort
} of remember, and it might get you started while someone else comes up with
} something. I was etching epoxy resins with saturated ethanolic NaOH to do
} PAS.
}
} Make a solution of saturated ethanolic NaOH by dumping a lot of NaOH
} pellets in a bottle of absolute ethanol, like an inch and a half in a pint
} bottle. Put it aside for a couple of weeks until it looks like cognac.
} Soak slides in this solution in a coplin jar for (and this is where I
} can't remember - Two hours? Two days?). Sections used to easily come off
} those old, plain slides, so I was careful not to agitate. I think they
} would stay on better with Superfrost Plus or treated slides. Go ahead and
} start making your cognac .. er.. etching solution and I'll look for my
} PAS protocol.
}
} Aloha from chilly Hawaii (OK, so it's 67F)
} Tina
}
}
}
} } Organization: Cooperative Oxford Laboratory
} }
} } Title-Subject: [Filtered] Paragon stain TEM
} }
} } Question: I have checked the listserver for Paragon staining of epoxy
} } resin and found several very helpful comments. I tried using
} } Martin's procedure without success. I came across another comment
} } about etching the slides first... Does anyone have experience with
} } this?
} }
} } I have since tried Richardson's stain and it just doesn't give enough
} } differentiation. I am staining coral tissues that have been
} } decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in
} } 1% Osmium and embedded in Spurr's.
} }
} } Hope you can help.
} } Sue
} }
} } Login Host: 205.156.36.37
} } ---------------------------------------------------------------------------
} }
} } ==============================Original Headers==============================
} } 8, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:23:26 2009
} } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0NPRl022677
} } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:23:26 -0600
} } 8, 11 -- Mime-Version: 1.0
} } 8, 11 -- Message-Id: {p06240801c5a6a764137a-at-[206.69.208.22]}
} } 8, 11 -- Date: Wed, 28 Jan 2009 18:23:25 -0600
} } 8, 11 -- To: microscopy-at-microscopy.com
} } 8, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver)
} } 8, 11 -- Subject: viaWWW: Paragon stain TEM
} } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } ==============================End of - Headers==============================
} }
} }
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
} ==============================Original Headers==============================
} 8, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 28 22:01:19 2009
} 8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
} 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T41IS7022534
} 8, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 22:01:18 -0600
} 8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
} 8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0T41Eox020137
} 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
} 8, 21 -- Wed, 28 Jan 2009 18:01:14 -1000 (HST)
} 8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0T41BjA020134;
} 8, 21 -- Wed, 28 Jan 2009 18:01:13 -1000 (HST)
} 8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
} 8, 21 -- Date: Wed, 28 Jan 2009 18:01:10 -1000 (HST)
} 8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} 8, 21 -- X-Sender: tina-at-halia
} 8, 21 -- To: sue.tyler-at-noaa.gov
} 8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
} 8, 21 -- Subject: Re: [Microscopy] viaWWW: Paragon stain TEM
} 8, 21 -- In-Reply-To: {200901290024.n0T0OCU6025080-at-ns.microscopy.com}
} 8, 21 -- Message-ID: {Pine.GSO.4.21.0901281752000.20054-100000-at-halia}
} 8, 21 -- MIME-Version: 1.0
} 8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
} ==============================End of - Headers==============================
}
}


--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)


==============================Original Headers==============================
5, 22 -- From smithj-at-winthrop.edu Thu Jan 29 07:52:47 2009
5, 22 -- Received: from messenger.winthrop.edu (messenger.winthrop.edu [199.79.254.177])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0TDqjh3022849
5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 07:52:46 -0600
5, 22 -- Received: from berlin.win.winthrop.edu (berlin.win.winthrop.edu [10.2.0.22])
5, 22 -- by messenger.winthrop.edu (MOS 3.8.6-GA)
5, 22 -- with ESMTP id GOG69277;
5, 22 -- Thu, 29 Jan 2009 08:52:43 -0500 (EST)
5, 22 -- Received: from lsb333-10.acc.winthrop.edu ([10.3.84.33]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959);
5, 22 -- Thu, 29 Jan 2009 08:51:38 -0500
5, 22 -- Message-ID: {4981B469.2010901-at-winthrop.edu}
5, 22 -- Date: Thu, 29 Jan 2009 08:51:37 -0500
5, 22 -- From: Julian Smith III {smithj-at-winthrop.edu}
5, 22 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
5, 22 -- MIME-Version: 1.0
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- Subject: Re: [Microscopy] Re: viaWWW: etching epoxy sections
5, 22 -- References: {200901290408.n0T48rie001107-at-ns.microscopy.com}
5, 22 -- In-Reply-To: {200901290408.n0T48rie001107-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- X-OriginalArrivalTime: 29 Jan 2009 13:51:38.0611 (UTC) FILETIME=[B51C0C30:01C98218]
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 29 Jan 2009 09:54:28 -0600
Subject: [Microscopy] LKB Knifemaker 7800 scoring wheels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Several people have now asked about the scoring wheel source. I have not
bought from this company, but I had found this when I searched a while
back. http://www.glasscuttingwheels.com/cw.html (what else would you
expect?)

I have measured the LKB wheels and they are 5.0mm dia, with a 1.4mm hole
and 1.1mm thick, so the wheels are correct. The edge angle this company
recommends for ~6mm thick glass is 144degrees and this is what the LKB
wheels look like; I can photograph and measure the angle with ImageJ if
anyone requests it. The axles may be more of an issue - the original
ones are 6.5mm long and that size is not avaialble. The mechanism can
only accommodate a 6.5mm length (it is precisely 7.0mm wide); they offer
a 4mm and a 9.25mmlength, but a machinist could easily grind the longer
one to length. The 4mm would just hold the wheel but not well. The old
axles in my box show significant wear so they probably should be
replaced as a pair, the way they were sold by LKB.

I still have a number of original, new, wheels and axles, if we need
them to make measurements.

Hope this helps.

Dale

Taylor, Jeannette wrote:
} Dear Dale, would you please send me your source for new scoring wheels? We have an LKB Knifemaker 7800 and it works fine but the scoring wheel needs to be replaced as it is getting dull.
}
} Thanks, Jeannette
}
} Jeannette Taylor
} Technologist II
} Robert P. Apkarian Integrated Electron Microscopy Core
} Emory University
} 1515 Dickey Drive
} Atlanta, Georgia 30322
}
} jvtaylo-at-emory.edu
} 404-712-8674
}
}
} This e-mail message (including any attachments) is for the sole use of
} the intended recipient(s) and may contain confidential and privileged
} information. If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination, distribution
} or copying of this message (including any attachments) is strictly
} prohibited.
}
} If you have received this message in error, please contact
} the sender by reply e-mail message and destroy all copies of the
} original message (including attachments).

==============================Original Headers==============================
6, 22 -- From dac-at-research.umass.edu Thu Jan 29 09:54:27 2009
6, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0TFsPFn008406
6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 09:54:27 -0600
6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
6, 22 -- (authenticated bits=0)
6, 22 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0TFsNJB005632
6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 10:54:23 -0500
6, 22 -- Message-ID: {4981D181.7040201-at-research.umass.edu}
6, 22 -- Date: Thu, 29 Jan 2009 10:55:45 -0500
6, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
6, 22 -- Reply-To: dac-at-research.umass.edu
6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
6, 22 -- MIME-Version: 1.0
6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 22 -- Subject: Re: [Microscopy] Re: LKB Knifemaker 7800 scoring wheels
6, 22 -- References: {200901282144.n0SLiteV001833-at-ns.microscopy.com} {99BAF15EB8A4F348949A061CACFD22B90119F3E75AFF-at-EXCHANGE20.Enterprise.emory.net}
6, 22 -- In-Reply-To: {99BAF15EB8A4F348949A061CACFD22B90119F3E75AFF-at-EXCHANGE20.Enterprise.emory.net}
6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Thu, 29 Jan 2009 16:56:05 -0600
Subject: [Microscopy] Re: viaWWW: etching epoxy sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ah, I found my old protocol, although I'll bet Julian's is more current,
and may give you the differentiation you're looking for.

Add 100-150g anhydrous NaOH pellets to 250 ml freshly opened bottle of
absolute ethanol. Allow to stand until cognac or deep rust-brown color,
shaking occasionally, for about a week. Keeps 4-5 weeks. Store in plastic
bottle, if possible. To remove resin from sections, immerse slides in
solution for an hour or more, checking to see when resin is etched away (I
remember this being about two hours for 0.5 micrometer thick sections).
Drain well, but don't blot. Immerse slides in 4 changes of absolute
ethanol, the proceed with staining, clearing, and mounting. This was
originally for PAS (Periodic Acid Schiff) on gecko reproductive
tissue. Now I want to try Julian's recipe on coral reproductive tissue...

Aloha,
Tina

} I prefer KOH in MeOH. We use 5' etch, followed by 2 changes of absolute
} MeOH. We published a protocol for Iron Hematoxylin/Eosin/Alcian blue
} (which gives pretty differentiated staining on marine inverts) a while
} back in Microskopie; I can send a .doc file to anyone interested.
} If you use PAS after etching, watch for staining artifact--epoxy
} embedding generates a bunch of 'em, at least with our invert material.
} Julian
}
} --
} Julian P.S. Smith III
} Director, Winthrop Microscopy Facility
} Dept. of Biology
} Winthrop University
} 520 Cherry Rd.
} Rock Hill, SC 29733
}
} 803-323-2111 x6427 (vox)
} 803-323-3448 (fax)
} 803-524-2347 (cell)

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
6, 21 -- From tina-at-pbrc.hawaii.edu Thu Jan 29 16:56:05 2009
6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0TMu4NB029533
6, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 16:56:05 -0600
6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0TMu1EQ023345
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
6, 21 -- Thu, 29 Jan 2009 12:56:02 -1000 (HST)
6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0TMu0tE023342;
6, 21 -- Thu, 29 Jan 2009 12:56:01 -1000 (HST)
6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
6, 21 -- Date: Thu, 29 Jan 2009 12:56:00 -1000 (HST)
6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
6, 21 -- X-Sender: tina-at-halia
6, 21 -- To: smithj-at-winthrop.edu
6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 21 -- Subject: Re: [Microscopy] viaWWW: etching epoxy sections
6, 21 -- In-Reply-To: {200901291354.n0TDs0P9024319-at-ns.microscopy.com}
6, 21 -- Message-ID: {Pine.GSO.4.21.0901291246440.23318-100000-at-halia}
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: ggt-at-phys.uni-sofia.bg
Date: Thu, 29 Jan 2009 18:23:26 -0600
Subject: [Microscopy] viaWWW: Hitachi S-550 schematics documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ggt-at-phys.uni-sofia.bg as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ggt-at-phys.uni-sofia.bg
Name: Gichka Georgieva

Organization: Sofia University

Title-Subject: [Filtered] Hitachi S-550 schematics documentation

Question: I need the full documentation (schematics especially) of a
SEM Hitachi S550 for upgrading and repair.

Can anyone help me to find it?

Thank you in advance.


Login Host: 62.44.98.162
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Jan 29 18:23:26 2009
9, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0U0NOiB016688
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 18:23:25 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c5a7f8ec310b-at-[206.69.208.22]}
9, 11 -- Date: Thu, 29 Jan 2009 18:23:23 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: ggt-at-phys.uni-sofia.bg (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Hitachi S-550 schematics documentation
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: eric-at-unquantum.net
Date: Thu, 29 Jan 2009 18:24:10 -0600
Subject: [Microscopy] viaWWW: Genie software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both eric-at-unquantum.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: eric-at-unquantum.net
Name: Eric Reiter

Organization: Unqunatum

Title-Subject: [Filtered] Genie software

Question: I have a Aptec series 5000 x-ray spectroscopy card:
Looking for

Aptec spectroscopy software, or
Canberra Genie software
for older 486-like computers in DOS or up to windows 2000.

Would like to buy this pulse height analysis software.

Login Host: 69.27.185.15
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jan 29 18:24:10 2009
8, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0U0O66m017366
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 18:24:09 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240803c5a7f90d38b6-at-[206.69.208.22]}
8, 11 -- Date: Thu, 29 Jan 2009 18:24:05 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: eric-at-unquantum.net (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Genie software
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 30 Jan 2009 10:53:10 -0600
Subject: [Microscopy] SEM holder for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

As there is again some demand here for observations of TEM grids by SEM,
I took out of the drawer the home made TEM grids holders I had machined
a while ago. And non of them is really practical to use. The first was
made with an EM300 tip, and works right, but it's a one shot and for
limited to the jeol 840 serie stage. The second is delicate to use, and
I fear to bent the grids each time I take them away from the holder.

So what kind of holder do other use for TEM grids (only SEM
observations, no STEM), which allows very short WD and an easy way to
mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues,
but certainly not at all sources. And users advices are very usfull !

I'm interested in any ideas and/or squetches for home manufacturing in
our workshop, and/or for documentions, users advices and manufacturer
doc and quoting etc.

Thanks in advance, and have a good WE !

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 30 10:53:10 2009
8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151])
8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0UGr9OB014699
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 10:53:09 -0600
8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
8, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n0UGr8Sr086858
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 17:53:08 +0100 (CET)
8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
8, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 29 -- (No client certificate requested)
8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 766C43EC02E
8, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 17:52:58 +0100 (CET)
8, 29 -- Message-ID: {49833069.2050502-at-ipcms.u-strasbg.fr}
8, 29 -- Date: Fri, 30 Jan 2009 17:52:57 +0100
8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
8, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
8, 29 -- MIME-Version: 1.0
8, 29 -- To: Microscopy-at-microscopy.com
8, 29 -- Subject: SEM holder for TEM grids
8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 29 -- Content-Transfer-Encoding: 8bit
8, 29 -- X-IPCMS-MailScanner: Found to be clean
8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.151]); Fri, 30 Jan 2009 17:53:08 +0100 (CET)
8, 29 -- X-Virus-Scanned: ClamAV 0.94.2/8924/Fri Jan 30 16:10:48 2009 on mr1.u-strasbg.fr
8, 29 -- X-Virus-Status: Clean
8, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
8, 29 -- autolearn=disabled version=3.2.5
8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr1.u-strasbg.fr
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 30 Jan 2009 11:03:10 -0600
Subject: [Microscopy] SEM holder for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

As there is again some demand here for observations of TEM grids by SEM,
I took out of the drawer the home made TEM grids holders I had machined
a while ago. And non of them is really practical to use. The first was
made with an EM300 tip, and works right, but it's a one shot and for
limited to the jeol 840 serie stage. The second is delicate to use, and
I fear to bent the grids each time I take them away from the holder.

So what kind of holder do other use for TEM grids (only SEM
observations, no STEM), which allows very short WD and an easy way to
mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues,
but certainly not at all sources. And users advices are very usfull !

I'm interested in any ideas and/or squetches for home manufacturing in
our workshop, and/or for documentions, users advices and manufacturer
doc and quoting etc.

Thanks in advance, and have a good WE !

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 30 11:03:10 2009
8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153])
8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0UH394k027846
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 11:03:10 -0600
8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
8, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n0UH38SA065122
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 18:03:08 +0100 (CET)
8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
8, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 29 -- (No client certificate requested)
8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 766C43EC02E
8, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 17:52:58 +0100 (CET)
8, 29 -- Message-ID: {49833069.2050502-at-ipcms.u-strasbg.fr}
8, 29 -- Date: Fri, 30 Jan 2009 17:52:57 +0100
8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
8, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
8, 29 -- MIME-Version: 1.0
8, 29 -- To: Microscopy-at-microscopy.com
8, 29 -- Subject: SEM holder for TEM grids
8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 29 -- Content-Transfer-Encoding: 8bit
8, 29 -- X-IPCMS-MailScanner: Found to be clean
8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 30 Jan 2009 18:03:08 +0100 (CET)
8, 29 -- X-Virus-Scanned: ClamAV 0.94.2/8924/Fri Jan 30 16:10:48 2009 on mr3.u-strasbg.fr
8, 29 -- X-Virus-Status: Clean
8, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
8, 29 -- autolearn=disabled version=3.2.5
8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr3.u-strasbg.fr
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 30 Jan 2009 12:04:56 -0600
Subject: [Microscopy] Re: TEM; diffraction pattern analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers,
I was asked recently about accuracy of measuring electron
diffraction patterns, and seemed to remember something in the MSA
listserver from about 10 years ago, forgetting that I was the one who
asked the question in the first place!

I'm not going for the record of longest running thread on the list, but
I do think it's worth asking the question again, particularly now there
are much better digital cameras (and aberration corrected machines), as
well as about 2^6 times more processing power in the standard computer -
which means 32-bit image processing is feasible, for example.

I don't expect any corrections to previous postings (Jan 1999) on the
basic physics (I hope) but it would be interesting to see how things
have moved on. Also I received 21 answers in 3 days back then, it will
be interesting to see if it's still as lively now.

And are there any other questions which were asked a decade ago which
should be re-visited?


Thanks

Richard Beanland

*Date: Tue, 05 Jan 1999 10:31:53 +0000 (GMT)


On Jan 30, 2009, at 9:06 AM, contact-at-integrityscientific.com wrote:

} I would like to get some information on TEM diffraction pattern
} analysis. Specifically;
}
} 1) What software is available for analysis of diffraction patterns
} (both
} ring patterns and spot patterns)? What kind of accuracy can be
} obtained
} - are we getting close to the accuracy of X-ray diffractometry yet, or
} are there fundamental reasons such as lens aberrations, smaller Bragg
} angles, and accuracy of measurement which mean that we'll never get
} there?
}
} 2) What are the typical procedures people use for, say, measuring
} camera
} length or identifying unknown phases using diffraction?
}
Dear Richard,
I certainly do not know all the existing software, but I do know that
there is the SP operation in SPIDER that can identify lattice points,
find the centers and radii of rings, and determine intensity values.
When I was doing SAED to determine the structure of phthalocyanines, I
wrote a script that performed a background subtraction in two steps.
The first step put boxes around all the spots, replaced the pixels
inside the boxes with values that were the average of the edges of the
boxes, then took a radial average (the center of which was the center
of the lattice). This was then subtracted from the ED pattern, which
got rid of the non-linear background and didn't have to be exact,
since the second step was a bilinear background subtraction. The
resulting intensities were sufficiently accurate to give reliable
structure determinations. I published several articles with Doug
Dorset and Jim Turner about this in the early '90s. Neither lens
aberrations, nor small Bragg angles present practical problems for
structure determinations, but dynamical scattering is a serious issue,
which was overcome in my work by operating at 1200 kV, which reduced
dynamical effects to a manageable level. Although I have never done
any CBED, I have heard reports at M&M detailing the information that
can be obtained, and these said that the low-order spots gave the most
accurate data on such features as the distribution of electrons in
chemical bonds. these data were more accurate than could be obtained
by any other method, including X-ray diffraction. John Spence is the
expert on this, so you may want to contact him.
I evaporated gold onto the phthalocyanines and took SAED patterns
from which the lattice constants of the phthalocyanines were
determined. (This also determines the camera length.) Having both
the gold and the specimen on the same grid controls for changes in
camera length that may occur with variations in specimen height or
other scope parameters. The phases were found by direct methods--in
the case of the phthalocyanines, the Sayre equation and triplet
formulas were sufficient, but either the tangent formula or maximum
entropy methods should work also. I authored a paper in Acta Cryst.
showing that these methods will work for electron diffraction, since
they are a consequence of the unitary nature of scattering processes.
The references in that paper are to work done by several people to
which I added a small amount.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Fri Jan 30 12:04:56 2009
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0UI4uCN025190
4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 12:04:56 -0600
4, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
4, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 600B432AD0C
4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 10:04:55 -0800 (PST)
4, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
4, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
4, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 15B1232AD40
4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 10:04:53 -0800 (PST)
4, 22 -- Message-Id: {1AD75A53-9FBE-4391-B672-7666D51C636D-at-caltech.edu}
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- To: microscopy-at-microscopy.com
4, 22 -- In-Reply-To: {200901301706.n0UH6XUF003345-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
4, 22 -- Subject: Re: [Microscopy] TEM; diffraction pattern analysis
4, 22 -- Date: Fri, 30 Jan 2009 10:04:53 -0800
4, 22 -- References: {200901301706.n0UH6XUF003345-at-ns.microscopy.com}
4, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 30 Jan 2009 18:22:49 -0600
Subject: [Microscopy] Re: SEM holder for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bonjour Jacques,

You probably already saw these in your search, but how about:

1. Grid holders, used in rotary shadowing might
be useful. Edwards, for example, makes a nice
holder that keeps 11 grids in place on a circular
holder. The holder could be inserted into the
SEM and much like a conventional stub. You can
see this device (E0857 1000 = 3 mm grid holder
used with Rotatilt 3) on page 2 and 3 of the
following brochure:

www.edwards.co.il/catalog/14/140200.pdf

2. The Grid Stick holder. It's a metal strip
designed to hold grids in place for TEM staining.
It does use a glue to tack the grids in place,
however. Otherwise, it looks reasonable. You need
to modify it to suit your needs. You can pursue
this at:

www.emsdiasum.com/microscopy/technical/datasheet/71178.aspx

3. Something like the Leica cryo-grid holder
might work. This is a clamp that holds onto the
edge of the grid and is held in place by a
tightened screw. You may be able to make one
using a single-edge razor blade. If the blade is
attached to a large stub by means of a screw
(such that the blade is flat against the stub
surface), the edge of the blade could be used to
hold the grids onto the stub. You can see the
cryo-grid holder here:

www.leica-ag.com/pdfs.nsf/(ALLIDs)/A02F29FF6B35118BC1256D5D001F8B32/$FILE/Leica_EMFCS-Brochure_EN.pdf

Check out the following refrence:

René Haas, Gilbert De Murcia . 1985. A simple
device for accurate and large scale rotary
shadowing of spread biological specimens. Journal
of Electron Microscopy Technique.
Volume 2, Issue 5 , Pages 519 - 520.

Good luck.

John Bozzola


} As there is again some demand here for observations of TEM grids by SEM,
} I took out of the drawer the home made TEM grids holders I had machined
} a while ago. And non of them is really practical to use. The first was
} made with an EM300 tip, and works right, but it's a one shot and for
} limited to the jeol 840 serie stage. The second is delicate to use, and
} I fear to bent the grids each time I take them away from the holder.

--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++


==============================Original Headers==============================
18, 21 -- From bozzola-at-siu.edu Fri Jan 30 18:22:49 2009
18, 21 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0V0MnaA019864
18, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 18:22:49 -0600
18, 21 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
18, 21 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n0V0Mluf020605
18, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 18:22:48 -0600 (CST)
18, 21 -- Mime-Version: 1.0
18, 21 -- Message-Id: {a0624081dc5a8e7022bdf-at-[131.230.177.136]}
18, 21 -- In-Reply-To: {200901301654.n0UGsMt8016167-at-ns.microscopy.com}
18, 21 -- References: {200901301654.n0UGsMt8016167-at-ns.microscopy.com}
18, 21 -- Date: Fri, 30 Jan 2009 18:22:53 -0600
18, 21 -- To: Microscopy-at-microscopy.com
18, 21 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
18, 21 -- Subject: Re: [Microscopy] SEM holder for TEM grids
18, 21 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
18, 21 -- X-Spam-Score: 0.00%
18, 21 -- X-MASF: 0.00%
18, 21 -- X-Whitelist: 0.00%
18, 21 -- Content-Transfer-Encoding: 8bit
18, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0V0MnaA019864
==============================End of - Headers==============================




From: blotocka-at-gmail.com
Date: Fri, 30 Jan 2009 19:06:18 -0600
Subject: [Microscopy] viaWWW: removing calcium oxalate crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both blotocka-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: blotocka-at-gmail.com
Name: Barbara £otocka

Organization: Warsaw Agricultural University

Title-Subject: [Filtered] removing calcium oxalate crystals

Question: Question:
Is there any procedure that allows to remove
(dissolve) calcium oxalate crystals from plant
samples prior to embedding?
I have to cut semi- and ultrathin sections of a
plant organ that is virtually studded with
druses. The hand sections look absolutely
stunning in polarization, but both paraffin and
hard-grade epoxy sections are scratched to shreds.
I considered soaking the osmium-contrasted
samples in some acidic buffer prior to
dehydration - but perhaps someone solved this
problem already?

Will be grateful for comments :-)
Barbara

Login Host: 89.77.150.78
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Fri Jan 30 19:06:18 2009
8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0V16Gsr005017
8, 13 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 19:06:18 -0600
8, 13 -- Mime-Version: 1.0
8, 13 -- Message-Id: {p06240801c5a954733f09-at-[206.69.208.22]}
8, 13 -- Date: Fri, 30 Jan 2009 19:06:15 -0600
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: blotocka-at-gmail.com (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: removing calcium oxalate crystals
8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 13 -- Content-Transfer-Encoding: 8bit
8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0V16Gsr005017
==============================End of - Headers==============================




From: andrewb-at-vsl.cua.edu
Date: Fri, 30 Jan 2009 20:23:11 -0600
Subject: [Microscopy] Re: SEM holder for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jacques,
I had a holder made in our shop some years back out of a 10mm dia
cylindrical carbon SEM stub which would accomodates 4 TEM grids. One could
start with a 25mm dia carbon stub and accomodate many more. Fabrication
involved milling 4 equallly spaced 3.1mm dia depressions 0.5-1.0mm deep in the
top of the stubso that the edge of each depression was about 1mm from the edge
of the stub.Then, a smaller diameter (~2.5mm dia) depression was bored about
10mm deep inthe center of each original depression to provide a small flange to
support the TEM grid and to act as as a beam "sink." A small radial notch about 0.5
mm wide was cut from the outer perimeter of the stub into the depression down to
a bit below the flange to allow tweezers to be used to place and remove each
grid. In my JSM-35 the evacuation of the airlock was gentle enough that no
retainers were needed to hold the grids in place. Later, we bored vents from
the side of the stub into the bottom of each inner depression to allow an
alternate path for the air to escape during pumpdown, and some small split
rings (similar to the old Philips circlip which can't be used here because it
is too stiff) were used to retain each grid.The rings were made by tightly wrapping
many turns of #32 tinned copper wire around a 3/32" drill bit and then
slitting the helix while still on the bit with a sharp razor blade along the
bit's length. Some adjustment and flattening was necessary to make the clip
fit snugly into the depression. These latter modifications were necessary
because when the holder was used in microscopes with a more vigorous pump-
down, the grids were sometimes blown out of the holder. If you don't need the
"sink" under the grid depression, you can omit it and possibly avoid the blowout
problem in a much simpler way. Perhaps this is more trouble than you want to go
to, but if you're interested contact me off list, and I can take a picture of the holder
to send to you.


On 30 Jan 2009 at 11:02, jacques.faerber-at-ipcms.u-strasbg.fr wrote:

}
} Hi all
}
} As there is again some demand here for observations of TEM grids by
} SEM, I took out of the drawer the home made TEM grids holders I had
} machined a while ago. And non of them is really practical to use. The
} first was made with an EM300 tip, and works right, but it's a one
} shot and for limited to the jeol 840 serie stage. The second is
} delicate to use, and I fear to bent the grids each time I take them
} away from the holder.
}
} So what kind of holder do other use for TEM grids (only SEM
} observations, no STEM), which allows very short WD and an easy way to
} mount/umount 5-6 grids in a batch ? I've soon looked in some
} catalogues, but certainly not at all sources. And users advices are
} very usfull !
}
} I'm interested in any ideas and/or squetches for home manufacturing in
} our workshop, and/or for documentions, users advices and manufacturer
} doc and quoting etc.
}
} Thanks in advance, and have a good WE !
}
} --
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
Sincerely yours,
Andy Buechele
Andrew C. Buechele, Ph.D.
The Catholic University of America - VSL
409 Hannan Hall
Washington, D.C. 20064
Phone: 202-319-4995 Fax: 202-319-4469




==============================Original Headers==============================
7, 30 -- From andrewb-at-vsl.cua.edu Fri Jan 30 20:23:11 2009
7, 30 -- Received: from mail.vsl.cua.edu (interface.vsl.cua.edu [136.242.188.2])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0V2N9gX021493
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 20:23:10 -0600
7, 30 -- Received: from localhost (localhost [127.0.0.1])
7, 30 -- by mail.vsl.cua.edu (Postfix) with ESMTP id 4C6459C1D09
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 21:23:09 -0500 (EST)
7, 30 -- X-Virus-Scanned: amavisd-new at vsl.cua.edu
7, 30 -- Received: from mail.vsl.cua.edu ([127.0.0.1])
7, 30 -- by localhost (pandora.vsl.cua.edu [127.0.0.1]) (amavisd-new, port 10024)
7, 30 -- with ESMTP id sWSt5SgzHNzj for {Microscopy-at-Microscopy.Com} ;
7, 30 -- Fri, 30 Jan 2009 21:23:07 -0500 (EST)
7, 30 -- Received: from [136.242.189.103] (unknown [136.242.189.103])
7, 30 -- by mail.vsl.cua.edu (Postfix) with ESMTP id 217349C1CFD
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 21:23:07 -0500 (EST)
7, 30 -- From: "Andrew Buechele" {andrewb-at-vsl.cua.edu}
7, 30 -- To: Microscopy-at-Microscopy.Com
7, 30 -- Date: Fri, 30 Jan 2009 21:23:06 -0500
7, 30 -- MIME-Version: 1.0
7, 30 -- Subject: Re: [Microscopy] SEM holder for TEM grids
7, 30 -- Reply-to: andrewb-at-vsl.cua.edu
7, 30 -- Message-ID: {49836FBA.10981.1F562EB-at-andrewb.vsl.cua.edu}
7, 30 -- Priority: normal
7, 30 -- In-reply-to: {200901301702.n0UH2vuU027567-at-ns.microscopy.com}
7, 30 -- References: {200901301702.n0UH2vuU027567-at-ns.microscopy.com}
7, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
7, 30 -- Content-type: text/plain; charset=ISO-8859-1
7, 30 -- Content-description: Mail message body
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n0V2N9gX021493
==============================End of - Headers==============================




From: sekkio-at-mac.com
Date: Sun, 1 Feb 2009 03:01:59 -0600
Subject: [Microscopy] July 11-15 2009 - Win in Science come to Genoa

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

EBSA 2009 is the 7th Congress of the European Biophysical Societies'
Association (EBSA), formed in 1984, with the objectives to advance and
disseminate knowledge of the principles, recent developments and
applications of biophysics, and to foster the exchange of scientific
information among biophysicists. EBSA2009 provides special incentives
for young investigators. The Congress will also celebrate 25 years of
EBSA.

Have a look to the Pleanry speakers list and Scientific program.
Bursaries for students will be available and Accommodations at 17
Euros per night including breakfast, too.

Visit www.ebsa2009.org.

All the best
Alby
----------------------------------------------------
"Water slowly flowed under the sky" (Cesare Pavese)
-----------------------------------------------------
Alberto Diaspro,
LAMBS IFOM IEO -MicroSCoBio, NBT-IIT, IBF-CNR
Department of Physics, University of Genoa,
Via Dodecaneso 33, 16146 Genoa, Italy -
fax +39-010314218 - tel +39 0103536426/309;
URLs: www.lambs.it;

----------------------------------------------

==============================Original Headers==============================
5, 20 -- From sekkio-at-mac.com Sun Feb 1 03:01:58 2009
5, 20 -- Received: from asmtpout018.mac.com (asmtpout018.mac.com [17.148.16.93])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1191wfY011645
5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 1 Feb 2009 03:01:58 -0600
5, 20 -- MIME-version: 1.0
5, 20 -- Content-transfer-encoding: 7BIT
5, 20 -- Content-type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 20 -- Received: from [10.0.1.3]
5, 20 -- (host49-140-dynamic.50-82-r.retail.telecomitalia.it [82.50.140.49])
5, 20 -- by asmtp018.mac.com
5, 20 -- (Sun Java(tm) System Messaging Server 6.3-7.03 (built Aug 7 2008; 32bit))
5, 20 -- with ESMTPSA id {0KED00BR2QF6R510-at-asmtp018.mac.com} for
5, 20 -- microscopy-at-microscopy.com; Sun, 01 Feb 2009 01:01:58 -0800 (PST)
5, 20 -- Message-id: {6AC04C2B-5B53-43C8-B8C4-0473D2A36F73-at-mac.com}
5, 20 -- From: Alberto Diaspro {sekkio-at-mac.com}
5, 20 -- To: microscopy-at-microscopy.com, by MicroscopyListServer {venu-at-purdue.edu}
5, 20 -- Reply-to: Alberto Diaspro {diaspro-at-fisica.unige.it} , staff-at-ebsa2009.org
5, 20 -- Subject: July 11-15 2009 - Win in Science come to Genoa
5, 20 -- Date: Sun, 01 Feb 2009 10:01:53 +0100
5, 20 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: mgengle-at-email.uky.edu
Date: Mon, 2 Feb 2009 14:16:42 -0600
Subject: [Microscopy] glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

MSA's Project MICRO (Microscopy In Curriculum - Research Outreach)
supports education about the microworld for middle school students.
That means a curriculum manual for teachers, volunteers to help in
classrooms, and advice and support in an extensive web page on MSA's
site: http://www.microscopy.org/ProjectMICRO. That page includes a
reviewed listing of over 150 children's books, other media, and
websites about microscopy and the microworld. Nanotechnology isn't
microscopy, but it certainly is part of the microworld. So MICRO
has just added a new database of all the books for the same age group
that MICRO has been able to find. Please take a look! The same
information will appear in Microscopy Today later this year.
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
1, 16 -- From schooley-at-mcn.org Sun Feb 1 15:03:06 2009
1, 16 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31])
1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n11L36bM028531
1, 16 -- for {microscopy-at-microscopy.com} ; Sun, 1 Feb 2009 15:03:06 -0600
1, 16 -- Received: from [66.81.64.252]
1, 16 -- by dns4.mcn.org with esmtpa (Exim 4.69)
1, 16 -- (envelope-from {schooley-at-mcn.org} )
1, 16 -- id 1LTjT7-0007Q2-3x
1, 16 -- for microscopy-at-microscopy.com; Sun, 01 Feb 2009 13:03:05 -0800
1, 16 -- Mime-Version: 1.0
1, 16 -- Message-Id: {a06200700c5abbeb7b1fe-at-[66.81.65.205]}
1, 16 -- Date: Sun, 1 Feb 2009 13:13:09 -0800
1, 16 -- To: microscopy-at-microscopy.com
1, 16 -- From: Caroline Schooley {schooley-at-mcn.org}
1, 16 -- Subject: Microscopy education
1, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From reginarus-at-shaw.ca.an Mon Feb 2 01:33:17 2009
Return-Path: {reginarus-at-shaw.ca.an}
Received: from google.com (p54A26945.dip.t-dialin.net [84.162.105.69])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n127XFm7003465
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 01:33:16 -0600
Received: from [175.131.115.172] (HELO google.com)
by scentedmeat.de; Mon, 2 Feb 2009 08:24:49 +0100

Hi All,

Regarding sources for the scoring wheel for LKB knifebreakers, I
received information that Leica Microsystems can also supply these
parts...................

the usual disclaimer, I have no connection with Leica...... Dale

} You can buy them from Leica.
} The order number is 16 70 52 27, 'scoring wheels for LKB knifemaker'
} Yes the part number is for wheel and axle set, 3 parts each per pack.
...............
} The rubber cushion we suggest not to use as it is not necessry.
} I did not get my email onto the Listserver as it bounced back !
}
} Pleased to be of assistance.
} Best Wishes,
} Ian
}
} Ian Lamswood
} Marketing Manager
} Hernalser Hauptstrasse 219
} 1170 Wien (Austria)
} From: Dale Callaham {dac-at-research.umass.edu}
} To: Ian Lamswood {ianlamswood44-at-yahoo.co.uk}
} Sent: Friday, 30 January, 2009 17:42:51
} Subject: Re: [Microscopy] LKB Knifemaker 7800 scoring wheels
}
} Hi Ian,
}
} Thanks! That solves a lot of problems. Is this part number for a wheel+axle set? Are other components also avaialble? The rubber shock cushion is another part people will likely want to replace regularly since they get stiff/dead over time. Has Leica taken over the LKB Knifebreaker service also?
}
} Did your message go to the Microscopy List? You should echo it there so people know that Leica is a source for parts.
}
} Dale





Kim Davidson wrote:
} Hi Dale,
}
} Would you share your source for the scoring wheel please?
}
} Thanks,
}
} kim
}
} ----------------------------------
} Kim Davidson
} Department of Biomedical Sciences
} Neurosciences Division
} 1617 Campus Delivery
} Colorado State University
} Fort Collins, CO 80523-1617
}
} 970-491-7389 (Phone)
} 970-491-7907 (FAX)
}
} kim.davidson-at-colostate.edu
} ----------------------------------
}
}
}
} dac-at-research.umass.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi Jon,
} }
} } The magic formula is the manual. You didn't mention having one. It goes
} } over all the features of the knives and adjustments to get good ones. It
} } is really very simple once you get into it - only about 4 or 5 things to
} } tweek.
} }
} } These things are fairly rugged. There is a trick to getting the movable
} } overhead clamping part off; You position the clamping lever at ~45o to
} } the front (or back??) and lift and it slides up and off. You may need to
} } clean the mating faces well with solvent to remove the sticky grease you
} } may find there. These parts are brass and only need to be clean. The
} } scoring part that you pull out is the same (clean) but I do use some
} } silicone lube lightly on it; never anything that would get gummy, and
} } some very light grease on the cam that the scoring wheel follows (inside
} } the silver outer tube; again here start with it clean, and use sparingly
} } a light grease only on the cam face, not the scoring wheel.
} }
} } Note that the score wheel can be set for cutting squares from strips
} } (the parallel lines) or scoring squares for knives - the square with a
} } line symbol; these settings determine where the scoring wheel drops onto
} } the glass and where it lifts off, very important.
} }
} } The twiddle knobs at the top and bottom of the glass-position clamping
} } forks position the glass square so that the { {fixed} } score falls on it
} } just so. At the top and bottom there are 2 adjustments one is side-side
} } and one clamps the metal fork that contacts the knife corner to set the
} } position of the glass for and aft; the front one sets the position and
} } the rear fork only applies pressure. You can change the angle of the
} } score relative to the corners, and control where the score lifts off the
} } square at the near corner by adjusting the clamping forks side-to-side
} } and fore and aft; the side-side can be done at both top and bottom to
} } accentuate your frustration but eventually place the score in the
} } correct position. Adjustment for a score to a position very close to the
} } near corner is critical for a controlled break; the break should come
} } out on the right-hand side of the near corner and within 0.5mm of the
} } corner - and this will be the cutting edge of the good knife. In good
} } adjustment you will get 2 knives that are fairly symmetrical but not
} } completely. The other opposite cutting edge will be less controlled and
} } that knife is rarely as good, but usually just fine for facing up blocks
} } or semi-thins.
} }
} } Scoring wheel must be sharp. Easily replaced. I have a source for them
} } if you need new ones. They are a standard size still available. Google
} } works too.
} }
} } Also the scoring pressure must be light - thus the need for the sharp
} } wheel; too much pressure, too deep and the score is broad and knives
} } will not break smoothly and be erratic in shape. A gentle breaking
} } pressure and slow break is preferred for the best knives.
} }
} } And all glass is not created equal. If you don't have a stock see if
} } someone can make a recommendation. I have a lot of old LKB glass and it
} } is still very good. People have brought newer glass and some of it does
} } not break so well.
} }
} } Let me know if you need a copy of the instructions w/pictures - the
} } originals with drawings of the score marks, angles, etc. are very helpful.
} }
} }
} } Dale
} }
} } jkrupp-at-deltacollege.edu wrote:
} }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Dear friends:
} } }
} } } This is a message asking for help adjusting our LKB Knifemaker(s).
} } }
} } } I am teaching an ultramicrotomy class to 20 community college students
} } } and we use glass knives. My job is to make sure the instruments are
} } } adjusted so they can make good knives.
} } }
} } } We have 6, yes that's six, LKB Knifemakers in various states of
} } } (dis)repair. I need to build at least one good one from all of these.
} } }
} } } Most of them were donated, some are better than others, I can probably
} } } figure out what to do, but just wanted to check with any experts on
} } } the list.
} } }
} } } In another life, I used an LKB Knifemaker that worked pretty well.
} } } That one was serviced by an LKB tech once and a while and, as usual,
} } } had all the adjustments taped down with notes saying things like 'Do
} } } not adjust'. Since I could at least follow directions, I never tried
} } } to change the adjustments.
} } }
} } } Now in my new life, I have 6 things that look like Knifemakers sitting
} } } on the bench and 20 sets of eyes looking at me expecting these things
} } } to work perfectly every time. I have done the expected Googling and
} } } found a few remarks about adjusting Knifemakers, but before I start
} } } tinkering, I wanted to check with the list to see if someone has the
} } } magic formula to insure success. Experience trumps google every time.
} } }
} } } Thanks
} } }
} } } Jon
} } }
} } } Jonathan Krupp
} } } Delta College
} } } 5151Pacific Ave.
} } } Stockton, CA 95207
} } } 209-954-5284
} } } jkrupp-at-deltacollege.edu
} } }
} } }
} } }
} } }
} } } ==============================Original Headers==============================
} } } 13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009
} } } 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
} } } 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937
} } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600
} } } 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
} } } 13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B
} } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
} } } 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
} } } 13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F
} } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT)
} } } 13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} } } 13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} } } 13, 42 -- with ESMTP id 45280969 for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 12:30:23 -0800
} } } 13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} } } 13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
} } } 13, 42 -- ESMTP id KE76WG00.4MS for {Microscopy-at-microscopy.com} ; Wed, 28
} } } 13, 42 -- Jan 2009 12:14:40 -0800
} } } 13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} } } 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 9F5C78F74D04
} } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} } } 13, 42 -- X-Virus-Scanned: amavisd-new at
} } } 13, 42 -- X-Spam-Flag: NO
} } } 13, 42 -- X-Spam-Score: -2.499
} } } 13, 42 -- X-Spam-Level:
} } } 13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
} } } 13, 42 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
} } } 13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} } } 13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
} } } 13, 42 -- with ESMTP id R34HTgsbNAlK for {Microscopy-at-microscopy.com} ;
} } } 13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} } } 13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74])
} } } 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5
} } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST)
} } } 13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu}
} } } 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} } } 13, 42 -- To: Microscopy-at-microscopy.com
} } } 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} } } 13, 42 -- Content-Transfer-Encoding: 7bit
} } } 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} } } 13, 42 -- Subject: LKB Knifemaker 7800
} } } 13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800
} } } 13, 42 -- X-Mailer: Apple Mail (2.930.3)
} } } ==============================End of - Headers==============================
} } }
} }
} } ==============================Original Headers==============================
} } 12, 20 -- From dac-at-research.umass.edu Wed Jan 28 15:44:31 2009
} } 12, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
} } 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLiUnn000740
} } 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:44:31 -0600
} } 12, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged))
} } 12, 20 -- (authenticated bits=0)
} } 12, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SLiTJx031617
} } 12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} } 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 16:44:30 -0500
} } 12, 20 -- Message-ID: {4980D1FA.6080608-at-research.umass.edu}
} } 12, 20 -- Date: Wed, 28 Jan 2009 16:45:30 -0500
} } 12, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
} } 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
} } 12, 20 -- MIME-Version: 1.0
} } 12, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
} } 12, 20 -- Subject: Re: [Microscopy] LKB Knifemaker 7800
} } 12, 20 -- References: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com}
} } 12, 20 -- In-Reply-To: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com}
} } 12, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 12, 20 -- Content-Transfer-Encoding: 7bit
} } ==============================End of - Headers==============================
} }

==============================Original Headers==============================
9, 23 -- From dac-at-research.umass.edu Mon Feb 2 10:53:02 2009
9, 23 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Gr0pe031194
9, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 10:53:01 -0600
9, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
9, 23 -- (authenticated bits=0)
9, 23 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n12GquFU010769
9, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
9, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 11:52:56 -0500
9, 23 -- Message-ID: {49872541.5070403-at-research.umass.edu}
9, 23 -- Date: Mon, 02 Feb 2009 11:54:25 -0500
9, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
9, 23 -- Reply-To: dac-at-research.umass.edu
9, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
9, 23 -- MIME-Version: 1.0
9, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 23 -- Subject: Re: [Microscopy] Re: LKB Knifemaker 7800 - another source for scoring
9, 23 -- wheels.
9, 23 -- References: {200901282145.n0SLjs5Y005102-at-ns.microscopy.com} {4980D3F3.3040804-at-lamar.colostate.edu}
9, 23 -- In-Reply-To: {4980D3F3.3040804-at-lamar.colostate.edu}
9, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 23 -- Content-Transfer-Encoding: 7bit
9, 23 -- X-Whitelist: TRUE
==============================End of - Headers==============================

From vicodin-at-hotmail.com Mon Feb 2 12:23:53 2009
Return-Path: {vicodin-at-hotmail.com}
Received: from google.com (adsl190-28-129-18.epm.net.co [190.28.129.18])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12INqNX018858
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 12:23:52 -0600
Received: from [151.196.113.82] (HELO google.com)
by soggy-putdown.co.uk; Mon, 2 Feb 2009 13:23:53 -0500

Hi listers,
We're having an issue with our EM grade glutaraldehyde. The last three batches of 2ml ampoules I've ordered have been very viscous and then form a sticky ppt when mixed with buffer. We have some 10ml ampoules that are fine. They have different lot numbers which makes me think it's batch dependent but before I return yet another box of glut, I was wondering if anyone else has had these issues. I've also noticed that the glut that isn't so thick becomes cloudy when first mixed and never has been before. If anyone has any ideas or has experienced the same things I'd like to hear from you.
Thank you,
Mary Gail Engle


Mary Gail Engle
Sr Research Facility Manager
Electron Microscopy & Imaging Facility
HSRB rm 001
Ph (859) 323-6108
FAX (859) 323-8089
BBSRB rm o74
Ph (859)323-2701
FAX (859) 257-1581
University of KY
Lexington, KY 40536




==============================Original Headers==============================
5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009
5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75])
5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12KGgDV025817
5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 14:16:42 -0600
5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52])
5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500
5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB02.ad.uky.edu
5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500
5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500
5, 24 -- Subject: glutaraldehyde
5, 24 -- Thread-Topic: glutaraldehyde
5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw==
5, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu}
5, 24 -- Accept-Language: en-US
5, 24 -- Content-Language: en-US
5, 24 -- X-MS-Has-Attach:
5, 24 -- X-MS-TNEF-Correlator:
5, 24 -- acceptlanguage: en-US
5, 24 -- Content-Type: text/plain; charset="us-ascii"
5, 24 -- MIME-Version: 1.0
5, 24 -- Content-Transfer-Encoding: 8bit
5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12KGgDV025817
==============================End of - Headers==============================




From: thoward-at-unm.edu
Date: Mon, 2 Feb 2009 15:01:12 -0600
Subject: [Microscopy] Re: glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've noticed the same thing with a recent box of 2-ml
ampoules, but I ran a test batch of cells up and they
appear to be fine by TEM. However, I put that lot away to
be used for X-gal & SEM (no TEM), just in case there is
something horribly wrong with it. I'm currently working
off of an older box that we had hidden away.

It will be interesting to see the responses on this one!

Tamara

On Mon, 2 Feb 2009 14:18:04 -0600
mgengle-at-email.uky.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi listers,
} We're having an issue with our EM grade glutaraldehyde.
} The last three batches of 2ml ampoules I've ordered have
} been very viscous and then form a sticky ppt when mixed
} with buffer. We have some 10ml ampoules that are fine.
} They have different lot numbers which makes me think
} it's batch dependent but before I return yet another box
} of glut, I was wondering if anyone else has had these
} issues. I've also noticed that the glut that isn't so
} thick becomes cloudy when first mixed and never has been
} before. If anyone has any ideas or has experienced the
} same things I'd like to hear from you.
} Thank you,
} Mary Gail Engle
}
}
} Mary Gail Engle
} Sr Research Facility Manager
} Electron Microscopy & Imaging Facility
} HSRB rm 001
} Ph (859) 323-6108
} FAX (859) 323-8089
} BBSRB rm o74
} Ph (859)323-2701
} FAX (859) 257-1581
} University of KY
} Lexington, KY 40536
}
}
}
}
} ==============================Original
} Headers==============================
} 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42
} 2009
} 5, 24 -- Received: from ironporta.uky.edu
} (ironporta.uky.edu [128.163.184.75])
} 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n12KGgDV025817
} 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb
} 2009 14:16:42 -0600
} 5, 24 -- Received: from ex7hb02.ad.uky.edu
} ([128.163.187.52])
} 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009
} 15:16:41 -0500
} 5, 24 -- Received: from EX7FM03.ad.uky.edu
} ([128.163.187.12]) by EX7HB02.ad.uky.edu
} 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009
} 15:16:40 -0500
} 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} 5, 24 -- To: "'microscopy-at-microscopy.com'"
} {microscopy-at-microscopy.com}
} 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500
} 5, 24 -- Subject: glutaraldehyde
} 5, 24 -- Thread-Topic: glutaraldehyde
} 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw==
} 5, 24 -- Message-ID:
} {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu}
} 5, 24 -- Accept-Language: en-US
} 5, 24 -- Content-Language: en-US
} 5, 24 -- X-MS-Has-Attach:
} 5, 24 -- X-MS-TNEF-Correlator:
} 5, 24 -- acceptlanguage: en-US
} 5, 24 -- Content-Type: text/plain; charset="us-ascii"
} 5, 24 -- MIME-Version: 1.0
} 5, 24 -- Content-Transfer-Encoding: 8bit
} 5, 24 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n12KGgDV025817
} ==============================End of -
} Headers==============================

***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

==============================Original Headers==============================
5, 24 -- From thoward-at-unm.edu Mon Feb 2 15:01:12 2009
5, 24 -- Received: from unm.edu (f5vs2.unm.edu [64.106.76.41])
5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12L1B3o008999
5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:01:12 -0600
5, 24 -- Received: from [192.168.1.33] (HELO unm.edu)
5, 24 -- by phact.unm.edu (CommuniGate Pro SMTP 4.3.6)
5, 24 -- with ESMTP id 162720042; Mon, 02 Feb 2009 14:01:11 -0700
5, 24 -- X-PMX-Host: Sabik
5, 24 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, FROM_EDU_TLD 0, RDNS_NXDOMAIN 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, WEBMAIL_SOURCE 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_HTTP_RECEIVED 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0'
5, 24 -- X-PMX-Version: x1 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.2.2.204659
5, 24 -- Received: from [64.234.154.122] (account thoward-at-unm.edu)
5, 24 -- by sabik.unm.edu (CommuniGate Pro WebUser 4.3.6)
5, 24 -- with HTTP id 29360172; Mon, 02 Feb 2009 14:01:10 -0700
5, 24 -- From: "Tamara A Howard" {thoward-at-unm.edu}
5, 24 -- Subject: Re: [Microscopy] glutaraldehyde
5, 24 -- To: mgengle-at-email.uky.edu, microscopy-at-microscopy.com
5, 24 -- X-Mailer: CommuniGate Pro WebUser Interface v.4.3.6
5, 24 -- Date: Mon, 02 Feb 2009 14:01:10 -0700
5, 24 -- Message-ID: {web-29360172-at-sabik.unm.edu}
5, 24 -- In-Reply-To: {200902022018.n12KI4Ju027619-at-ns.microscopy.com}
5, 24 -- References: {200902022018.n12KI4Ju027619-at-ns.microscopy.com}
5, 24 -- MIME-Version: 1.0
5, 24 -- Content-Type: text/plain; charset="ISO-8859-1"; format="flowed"
5, 24 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: rcmoretz-at-gmail.com
Date: Mon, 2 Feb 2009 15:02:56 -0600
Subject: [Microscopy] Re: glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mary:

Have you contacted the vendor? I always found the vendors to be very
responsive when I had an issue with a product.

Roger Moretz, Ph.D., ret.

On Mon, Feb 2, 2009 at 3:21 PM, {mgengle-at-email.uky.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi listers,
} We're having an issue with our EM grade glutaraldehyde. The last three batches of 2ml ampoules I've ordered have been very viscous and then form a sticky ppt when mixed with buffer. We have some 10ml ampoules that are fine. They have different lot numbers which makes me think it's batch dependent but before I return yet another box of glut, I was wondering if anyone else has had these issues. I've also noticed that the glut that isn't so thick becomes cloudy when first mixed and never has been before. If anyone has any ideas or has experienced the same things I'd like to hear from you.
} Thank you,
} Mary Gail Engle
}
}
} Mary Gail Engle
} Sr Research Facility Manager
} Electron Microscopy & Imaging Facility
} HSRB rm 001
} Ph (859) 323-6108
} FAX (859) 323-8089
} BBSRB rm o74
} Ph (859)323-2701
} FAX (859) 257-1581
} University of KY
} Lexington, KY 40536
}
}
}
}
} ==============================Original Headers==============================
} 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009
} 5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75])
} 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12KGgDV025817
} 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 14:16:42 -0600
} 5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52])
} 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500
} 5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB02.ad.uky.edu
} 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500
} 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} 5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500
} 5, 24 -- Subject: glutaraldehyde
} 5, 24 -- Thread-Topic: glutaraldehyde
} 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw==
} 5, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu}
} 5, 24 -- Accept-Language: en-US
} 5, 24 -- Content-Language: en-US
} 5, 24 -- X-MS-Has-Attach:
} 5, 24 -- X-MS-TNEF-Correlator:
} 5, 24 -- acceptlanguage: en-US
} 5, 24 -- Content-Type: text/plain; charset="us-ascii"
} 5, 24 -- MIME-Version: 1.0
} 5, 24 -- Content-Transfer-Encoding: 8bit
} 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12KGgDV025817
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
5, 35 -- From rcmoretz-at-gmail.com Mon Feb 2 15:02:56 2009
5, 35 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.170])
5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12L2tBo012298
5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:02:56 -0600
5, 35 -- Received: by wf-out-1314.google.com with SMTP id 27so1629475wfd.21
5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 02 Feb 2009 13:02:55 -0800 (PST)
5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 35 -- d=gmail.com; s=gamma;
5, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
5, 35 -- :date:message-id:subject:from:to:content-type
5, 35 -- :content-transfer-encoding;
5, 35 -- bh=WSd1ILRNs0JUKk3BGbDoGZbPQg73jTmj5+G1wpvyER8=;
5, 35 -- b=goXedM+MxU3qv66rPAh4B80DqqjEGWNBG6+tMWeSUs0pFAnvruheILpHxfrUXBB99Q
5, 35 -- MYetJ1iKG4UB5jClAAX49cRW4lo+kMU+Sh8Hprw3szdduERt/8b/MygVRf7edD25cgk7
5, 35 -- 4ccZ6j2i5s/6C0kniNATy6EEow3KPDRaMmngY=
5, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 35 -- d=gmail.com; s=gamma;
5, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
5, 35 -- :content-type:content-transfer-encoding;
5, 35 -- b=jwlmard5D3hDXrPoonRaFJL7JbnnoYpwZQ0INW1lp0pNPLYxKWqpCkuwoHt1JLr5nJ
5, 35 -- pHWhxeOWl+nG7nT5dSRYE4Kz6Jeis2pX9nz3/qdvfPrksv4OeWjpiS5azzQ+5mx03B2G
5, 35 -- trjCE4y+FqKFIKJhhVqxPAAvpqJ/gcXZ6ONvI=
5, 35 -- MIME-Version: 1.0
5, 35 -- Received: by 10.143.160.17 with SMTP id m17mr1972586wfo.298.1233608575351;
5, 35 -- Mon, 02 Feb 2009 13:02:55 -0800 (PST)
5, 35 -- In-Reply-To: {200902022021.n12KLkho032711-at-ns.microscopy.com}
5, 35 -- References: {200902022021.n12KLkho032711-at-ns.microscopy.com}
5, 35 -- Date: Mon, 2 Feb 2009 16:02:55 -0500
5, 35 -- Message-ID: {950e3cfd0902021302x2987fb2ai7ad92b26047598ae-at-mail.gmail.com}
5, 35 -- Subject: Re: [Microscopy] glutaraldehyde
5, 35 -- From: Roger Moretz {rcmoretz-at-gmail.com}
5, 35 -- To: mgengle-at-email.uky.edu, Microscopy Listserv {Microscopy-at-microscopy.com}
5, 35 -- Content-Type: text/plain; charset=ISO-8859-1
5, 35 -- Content-Transfer-Encoding: 8bit
5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12L2tBo012298
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Mon, 2 Feb 2009 15:46:02 -0600
Subject: [Microscopy] Re: glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In response to some of your comments, these vials are 70% glut, in the sealed ampoules, not opened until ready to use. I've been doing EM since 1971 (oops, telling my age) and have always prepared fix the same way and have never had a problem until now. We keep it in the refrigerator and these last three orders are from 2 weeks ago and maybe 6 months ago so they're fairly new. The vendor was kind enough to replace them but the latest batches are doing the same thing. Like one of the people who wrote, we're hoarding our last good box.

Mary Gail Engle
Sr Research Facility Manager
Electron Microscopy & Imaging Facility
HSRB rm 001
Ph (859) 323-6108
FAX (859) 323-8089
BBSRB rm o74
Ph (859)323-2701
FAX (859) 257-1581
University of KY
Lexington, KY 40536




==============================Original Headers==============================
4, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 15:14:07 2009
4, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12LE641004052
4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:14:07 -0600
4, 24 -- Received: from ex7hb01.ad.uky.edu ([128.163.187.50])
4, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 16:14:06 -0500
4, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB01.ad.uky.edu
4, 24 -- ([128.163.187.50]) with mapi; Mon, 2 Feb 2009 16:14:03 -0500
4, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
4, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
4, 24 -- Date: Mon, 2 Feb 2009 16:14:03 -0500
4, 24 -- Subject: glutaraldehyde
4, 24 -- Thread-Topic: glutaraldehyde
4, 24 -- Thread-Index: AcmFeysaDIX0dKIGT+6ZNyQVZTbDMg==
4, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5726-at-EX7FM03.ad.uky.edu}
4, 24 -- Accept-Language: en-US
4, 24 -- Content-Language: en-US
4, 24 -- X-MS-Has-Attach:
4, 24 -- X-MS-TNEF-Correlator:
4, 24 -- acceptlanguage: en-US
4, 24 -- Content-Type: text/plain; charset="us-ascii"
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Transfer-Encoding: 8bit
4, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12LE641004052
==============================End of - Headers==============================

From hotdak.netpionavaro2001-at-hotmail.com Mon Feb 2 15:43:05 2009
Return-Path: {hotdak.netpionavaro2001-at-hotmail.com}
Received: from google.com (5ad569a1.bb.sky.com [90.213.105.161])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Lh3sk018553
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 15:43:04 -0600
Received: from [181.56.37.82] (HELO google.com)
by busy-gee.cn; Mon, 2 Feb 2009 21:43:03 +0000
Message-ID: {00000007F4216231691959478}
Reply-To: Richie Whitehead {wiborg.ken17148-at-gmail.com}

Hi Mary Gail,

We've heard of similar problems with EM grade glutaraldehyde. Based
on our experience with glutaraldehyde we think it could be
temperature issues during production.

When we started doing glutaraldehyde over 50 years ago we hit a
number of snags. It took us several years to get our production down pat.

It's not always easy providing assistance to one's competitor but,
because of the importance of this issue, if you have whoever supplied
the product call us we'll do what we can.

John Arnott

Disclaimer: Ladd Research supplies EM products including glutaraldehyde

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 03:22 PM 2/2/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
15, 25 -- From jd-at-laddresearch.com Mon Feb 2 15:46:02 2009
15, 25 -- Received: from cernan.electric.net (cernan.electric.net [72.35.23.19])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Lk2bS018685
15, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:46:02 -0600
15, 25 -- Received: from 1LU6cD-0000S1-VZ by cernan.electric.net with emc1-ok (Exim 4.69)
15, 25 -- (envelope-from {jd-at-laddresearch.com} )
15, 25 -- id 1LU6cE-0000TP-Td; Mon, 02 Feb 2009 13:46:02 -0800
15, 25 -- Received: by emcmailer; Mon, 02 Feb 2009 13:46:02 -0800
15, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
15, 25 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256)
15, 25 -- (Exim 4.69)
15, 25 -- (envelope-from {jd-at-laddresearch.com} )
15, 25 -- id 1LU6cD-0000S1-VZ; Mon, 02 Feb 2009 13:46:02 -0800
15, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
15, 25 -- Date: Mon, 02 Feb 2009 16:45:58 -0500
15, 25 -- To: mgengle-at-email.uky.edu
15, 25 -- From: jd {jd-at-laddresearch.com}
15, 25 -- Subject: Re: [Microscopy] glutaraldehyde
15, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
15, 25 -- Mime-Version: 1.0
15, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
15, 25 -- X-Outbound-IP: 216.204.198.170
15, 25 -- X-Env-From: jd-at-laddresearch.com
15, 25 -- X-Virus-Status: Scanned by VirusSMART (c)
15, 25 -- Message-Id: {E1LU6cE-0000TP-Td-at-cernan.electric.net}
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Mon, 2 Feb 2009 16:13:26 -0600
Subject: [Microscopy] glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We had this problem too, but solved it by making sure both the concentrated
glut. and the buffer it's going into are warmed a little, then no problems
mixing them and no precipitate forms when the dilute fixative is put back in
the fridge. Not sure of the chemistry behind this, but it is worse when
going into cold phosphate buffer rather than cold PIPES buffer. And the
viscous stuff in 2 ml ampoules is 70% glut., as mentioned by someone else.
We mainly do light microscopy and have not noticed that this fixative does a
different job to earlier batches (usually something else is the problem, not
the fixative....).
cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 3/02/09 8:50 AM, "jd-at-laddresearch.com" {jd-at-laddresearch.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Mary Gail,
}
} We've heard of similar problems with EM grade glutaraldehyde. Based
} on our experience with glutaraldehyde we think it could be
} temperature issues during production.
}
} When we started doing glutaraldehyde over 50 years ago we hit a
} number of snags. It took us several years to get our production down pat.
}
} It's not always easy providing assistance to one's competitor but,
} because of the importance of this issue, if you have whoever supplied
} the product call us we'll do what we can.
}
} John Arnott
}
} Disclaimer: Ladd Research supplies EM products including glutaraldehyde
}
} Ladd Research
} 83 Holly Court
} Williston, VT 05495
}
} On-line Catalog: www.laddresearch.com
}
} Telephone: 1-802-658-4961 (anywhere)
} Toll Free 1-800-451-3406 (US)
} Fax: 1-802-660-8859
}
} e-mail: sales-at-laddresearch.com
}
} At 03:22 PM 2/2/2009, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi listers,
} } We're having an issue with our EM grade glutaraldehyde. The last
} } three batches of 2ml ampoules I've ordered have been very viscous
} } and then form a sticky ppt when mixed with buffer. We have some
} } 10ml ampoules that are fine. They have different lot numbers which
} } makes me think it's batch dependent but before I return yet another
} } box of glut, I was wondering if anyone else has had these
} } issues. I've also noticed that the glut that isn't so thick
} } becomes cloudy when first mixed and never has been before. If
} } anyone has any ideas or has experienced the same things I'd like to
} } hear from you.
} } Thank you,
} } Mary Gail Engle
} }
} }
} } Mary Gail Engle
} } Sr Research Facility Manager
} } Electron Microscopy & Imaging Facility
} } HSRB rm 001
} } Ph (859) 323-6108
} } FAX (859) 323-8089
} } BBSRB rm o74
} } Ph (859)323-2701
} } FAX (859) 257-1581
} } University of KY
} } Lexington, KY 40536
} }
} }
} }
} }
} } ==============================Original Headers==============================
} } 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009
} } 5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu
} } [128.163.184.75])
} } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n12KGgDV025817
} } 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009
} } 14:16:42 -0600
} } 5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52])
} } 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500
} } 5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by
} } EX7HB02.ad.uky.edu
} } 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500
} } 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} } 5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} } 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500
} } 5, 24 -- Subject: glutaraldehyde
} } 5, 24 -- Thread-Topic: glutaraldehyde
} } 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw==
} } 5, 24 -- Message-ID:
} } {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu}
} } 5, 24 -- Accept-Language: en-US
} } 5, 24 -- Content-Language: en-US
} } 5, 24 -- X-MS-Has-Attach:
} } 5, 24 -- X-MS-TNEF-Correlator:
} } 5, 24 -- acceptlanguage: en-US
} } 5, 24 -- Content-Type: text/plain; charset="us-ascii"
} } 5, 24 -- MIME-Version: 1.0
} } 5, 24 -- Content-Transfer-Encoding: 8bit
} } 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n12KGgDV025817
} } ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 15, 25 -- From jd-at-laddresearch.com Mon Feb 2 15:46:02 2009
} 15, 25 -- Received: from cernan.electric.net (cernan.electric.net
} [72.35.23.19])
} 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n12Lk2bS018685
} 15, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:46:02 -0600
} 15, 25 -- Received: from 1LU6cD-0000S1-VZ by cernan.electric.net with emc1-ok
} (Exim 4.69)
} 15, 25 -- (envelope-from {jd-at-laddresearch.com} )
} 15, 25 -- id 1LU6cE-0000TP-Td; Mon, 02 Feb 2009 13:46:02 -0800
} 15, 25 -- Received: by emcmailer; Mon, 02 Feb 2009 13:46:02 -0800
} 15, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
} 15, 25 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256)
} 15, 25 -- (Exim 4.69)
} 15, 25 -- (envelope-from {jd-at-laddresearch.com} )
} 15, 25 -- id 1LU6cD-0000S1-VZ; Mon, 02 Feb 2009 13:46:02 -0800
} 15, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 15, 25 -- Date: Mon, 02 Feb 2009 16:45:58 -0500
} 15, 25 -- To: mgengle-at-email.uky.edu
} 15, 25 -- From: jd {jd-at-laddresearch.com}
} 15, 25 -- Subject: Re: [Microscopy] glutaraldehyde
} 15, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
} 15, 25 -- Mime-Version: 1.0
} 15, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} 15, 25 -- X-Outbound-IP: 216.204.198.170
} 15, 25 -- X-Env-From: jd-at-laddresearch.com
} 15, 25 -- X-Virus-Status: Scanned by VirusSMART (c)
} 15, 25 -- Message-Id: {E1LU6cE-0000TP-Td-at-cernan.electric.net}
} ==============================End of - Headers==============================



==============================Original Headers==============================
8, 44 -- From prvs=Rosemary.White=2772c1f18-at-csiro.au Mon Feb 2 16:13:26 2009
8, 44 -- Received: from act-MTAout4.csiro.au (act-MTAout4.csiro.au [150.229.7.41])
8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12MDOxa000591
8, 44 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 16:13:25 -0600
8, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
8, 44 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
8, 44 -- s=email; t=1233612805; x=1265148805;
8, 44 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
8, 44 -- mime-version:content-transfer-encoding:content-id:
8, 44 -- content-description:resent-date:resent-from:resent-sender:
8, 44 -- resent-to:resent-cc:resent-message-id:in-reply-to:
8, 44 -- references:list-id:list-help:list-unsubscribe:
8, 44 -- list-subscribe:list-post:list-owner:list-archive;
8, 44 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
8, 44 -- |Subject:=20Re:=20[Microscopy]=20Re:=20glutaraldehyde
8, 44 -- |Date:=20Tue,=203=20Feb=202009=2009:13:21=20+1100
8, 44 -- |Message-ID:=20 {C5ADBB31.30A5%rosemary.white-at-csiro.au}
8, 44 -- |To:=20"jd-at-laddresearch.com"=20 {jd-at-laddresearch.com} ,=20 {
8, 44 -- microscopy-at-microscopy.com} |MIME-Version:=201.0
8, 44 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200902
8, 44 -- 022150.n12LooAJ027841-at-ns.microscopy.com} ;
8, 44 -- bh=qJzC9UZLZRjOC6Uqk8Q89HIOQra7BKkwuWN5sZ8Dm7A=;
8, 44 -- b=dCWODSE5GeEFYmSpe4HnBr3xWN+ZnpllOaqx5FPif4i7PqARx0d7WHXG
8, 44 -- Gh743mK2AqEHVYvmDvQjsGN7c0wrZYLArIJl09+etaYI3UueNDZaH7xsX
8, 44 -- Cc+zwdydAG1FO05;
8, 44 -- X-IronPort-AV: E=Sophos;i="4.37,367,1231074000";
8, 44 -- d="scan'208";a="20079862"
8, 44 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
8, 44 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 03 Feb 2009 09:13:23 +1100
8, 44 -- Received: from [152.83.167.123] (152.83.167.123) by
8, 44 -- EXNSW-HTCA01.nexus.csiro.au (130.155.117.126) with Microsoft SMTP Server id
8, 44 -- 8.1.311.2; Tue, 3 Feb 2009 09:13:23 +1100
8, 44 -- User-Agent: Microsoft-Entourage/12.10.0.080409
8, 44 -- Date: Tue, 3 Feb 2009 09:13:21 +1100
8, 44 -- Subject: Re: [Microscopy] Re: glutaraldehyde
8, 44 -- From: Rosemary White {rosemary.white-at-csiro.au}
8, 44 -- To: "jd-at-laddresearch.com" {jd-at-laddresearch.com} , {microscopy-at-microscopy.com}
8, 44 -- Message-ID: {C5ADBB31.30A5%rosemary.white-at-csiro.au}
8, 44 -- Thread-Topic: [Microscopy] Re: glutaraldehyde
8, 44 -- Thread-Index: AcmFgFZFPF2SdrHRQXKJHCKzpl9O6wAAx7o3
8, 44 -- In-Reply-To: {200902022150.n12LooAJ027841-at-ns.microscopy.com}
8, 44 -- MIME-Version: 1.0
8, 44 -- Content-Type: text/plain; charset="US-ASCII"
8, 44 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jean-paul.bailon-at-polymtl.ca
Date: Tue, 3 Feb 2009 11:31:19 -0600
Subject: [Microscopy] SEM holder for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bonjour Jacques,

Have a look at a recent paper signed by W. Vanderline in the journal
"Microscopy Today" (vol. 16, n° 6, Nov. 2008, p 28-35). A holder for doing
STEM-in-SEM is described in details. It is actually sold under license by
Ernest Fullam Inc. (items 18300 or 18301)
(http://www.fullam.com/Semacces.htm#holders). Your machine shop would be
probably inspirited by this holder if you choose a home-made approach which
is probably less costly.

Cordiales salutations.

Jean-Paul Baïlon

++++++++++++++++++++++++++++++++++++++
Prof. Jean-Paul Baïlon jean-paul.bailon-at-polymtl.ca
{mailto:jean-paul.bailon-at-polymtl.ca}
Génie mécanique Tél: +1(514) 340 4711, p. 4260
École Polytechnique Fax: +1(514) 340 4468
CP 6079, Succursale Centre-Ville
Montréal (QC) Canada H3C 3A7
++++++++++++++++++++++++++++++++++++++






==============================Original Headers==============================
6, 25 -- From jean-paul.bailon-at-polymtl.ca Tue Feb 3 11:31:19 2009
6, 25 -- Received: from smtp.polymtl.ca (smtp.polymtl.ca [132.207.4.11])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13HVJmo026643
6, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 11:31:19 -0600
6, 25 -- Received: from jpbailon (jmdorlot.metal.polymtl.ca [132.207.53.29])
6, 25 -- by smtp.polymtl.ca (8.14.3/8.14.3) with ESMTP id n13HVEpP016413
6, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 12:31:18 -0500
6, 25 -- Return-Receipt-To: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca}
6, 25 -- Reply-To: {jean-paul.bailon-at-polymtl.ca}
6, 25 -- From: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- Subject: SEM holder for TEM grids
6, 25 -- Date: Tue, 3 Feb 2009 12:31:16 -0500
6, 25 -- Organization: Ecole Polytechnique de Montreal
6, 25 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAIv5ENKzUdMRv4cAUAR8QvvCgAAAEAAAADtcZNkqWKRPgzrJpcA5rAABAAAAAA==-at-polymtl.ca}
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="iso-8859-1"
6, 25 -- X-Mailer: Microsoft Office Outlook 11
6, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
6, 25 -- Thread-Index: AcmGJTf3jka5Cir0SnGH0zURrNYIag==
6, 25 -- Disposition-Notification-To: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca}
6, 25 -- X-Poly-FromMTA: (jmdorlot.metal.polymtl.ca [132.207.53.29]) at Tue, 3 Feb 2009 17:31:14 +0000
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13HVJmo026643
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Tue, 3 Feb 2009 13:20:40 -0600
Subject: [Microscopy] Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I've been having trouble with 3mm/1/8"carbon rods for my Edwards 306 coater since running
out of my stash of Union Carbide National Carbon Company "Spectroscopic Electrodes" a
while ago.

I don't have a reliably calibrated vacuum gauge in the 306, but I suspect that the vacuum
achieved isn't that great, however, it's as good as it was when I was getting great coating
from the previous rods.

I bought some "graphite" rods from one supplier, but they needed a higher current and
temperature than the 306 could achieve. I then bought some "amorphous carbon" rods, but
the success rate isn't very high, there just doesn't seem to be much coating produced.

As I understand it, all rods contain a greater or lesser ratio of graphite and amorphous
carbon, and the greater the graphite content, the higher the temperature required for coating.

Does anyone know of a supplier of rods identical or similar to the old Union Carbide National
Carbon Company ones?

cheers
Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
9, 26 -- From r.sims-at-auckland.ac.nz Tue Feb 3 13:20:40 2009
9, 26 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13JKZ8w012509
9, 26 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Feb 2009 13:20:39 -0600
9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1])
9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 385979E9C4
9, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 4 Feb 2009 08:20:34 +1300 (NZDT)
9, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
9, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
9, 26 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
9, 26 -- with ESMTP id YtGqwpgaEG92 for {Microscopy-at-Microscopy.Com} ;
9, 26 -- Wed, 4 Feb 2009 08:20:34 +1300 (NZDT)
9, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1D0AD9E9BE
9, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 4 Feb 2009 08:20:33 +1300 (NZDT)
9, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
9, 26 -- To: Microscopy-at-Microscopy.Com
9, 26 -- Date: Wed, 04 Feb 2009 08:20:33 +1300
9, 26 -- MIME-Version: 1.0
9, 26 -- Subject: Carbon Rods
9, 26 -- Message-ID: {49894FD1.10600.48DDDE-at-r.sims.auckland.ac.nz}
9, 26 -- Priority: normal
9, 26 -- X-mailer: Pegasus Mail for Windows (4.41)
9, 26 -- Content-type: text/plain; charset=US-ASCII
9, 26 -- Content-transfer-encoding: 7BIT
9, 26 -- Content-description: Mail message body
==============================End of - Headers==============================




From: mgengle-at-email.uky.edu
Date: Tue, 3 Feb 2009 14:26:50 -0600
Subject: [Microscopy] glutaraldehyde issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before.

Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems.

So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal.


Mary Gail Engle
Sr Research Facility Manager
Electron Microscopy & Imaging Facility
HSRB rm 001
Ph (859) 323-6108
FAX (859) 323-8089
BBSRB rm o74
Ph (859)323-2701
FAX (859) 257-1581
University of KY
Lexington, KY 40536




==============================Original Headers==============================
8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009
8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089
8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600
8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55])
8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500
8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu
8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500
8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500
8, 24 -- Subject: glutaraldehyde issue
8, 24 -- Thread-Topic: glutaraldehyde issue
8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ==
8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu}
8, 24 -- Accept-Language: en-US
8, 24 -- Content-Language: en-US
8, 24 -- X-MS-Has-Attach:
8, 24 -- X-MS-TNEF-Correlator:
8, 24 -- acceptlanguage: en-US
8, 24 -- Content-Type: text/plain; charset="us-ascii"
8, 24 -- MIME-Version: 1.0
8, 24 -- Content-Transfer-Encoding: 8bit
8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Tue, 3 Feb 2009 15:02:48 -0600
Subject: [Microscopy] Re: glutaraldehyde issue - vendor responsibility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't know who the vendor is, but I be very reluctant to ever order
from them again. It is a terrible thing to ship product when it is known
that production issues exist. The time that people waste on compromised
fixations and troubleshooting the problem is a huge loss and sometimes
the loss is not recoverable. To {not} disclose the vendor leaves many
others at risk of wasted time and lost experiments. We pay dearly for
what should be trusted chemicals to do our work and vendors shipping bad
product really should not be protected from exposure. They know the lot
numbers and who received their product and yet I haven't heard that
anyone got a notice of recall, or an email to this list to get the word
out. We owe it to each other as fellow microscopists to keep the shades
open and let in the light.

Dale Callaham



mgengle-at-email.uky.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear listers,
}
} I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before.
}
} Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems.
}
} So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal.
}
}
} Mary Gail Engle
} Sr Research Facility Manager
} Electron Microscopy & Imaging Facility
} HSRB rm 001
} Ph (859) 323-6108
} FAX (859) 323-8089
} BBSRB rm o74
} Ph (859)323-2701
} FAX (859) 257-1581
} University of KY
} Lexington, KY 40536
}
}
}
}
} ==============================Original Headers==============================
} 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009
} 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76])
} 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089
} 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600
} 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55])
} 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500
} 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu
} 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500
} 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500
} 8, 24 -- Subject: glutaraldehyde issue
} 8, 24 -- Thread-Topic: glutaraldehyde issue
} 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ==
} 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu}
} 8, 24 -- Accept-Language: en-US
} 8, 24 -- Content-Language: en-US
} 8, 24 -- X-MS-Has-Attach:
} 8, 24 -- X-MS-TNEF-Correlator:
} 8, 24 -- acceptlanguage: en-US
} 8, 24 -- Content-Type: text/plain; charset="us-ascii"
} 8, 24 -- MIME-Version: 1.0
} 8, 24 -- Content-Transfer-Encoding: 8bit
} 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089
} ==============================End of - Headers==============================

==============================Original Headers==============================
5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009
5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913
5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600
5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
5, 22 -- (authenticated bits=0)
5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347
5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500
5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu}
5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500
5, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
5, 22 -- Reply-To: dac-at-research.umass.edu
5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
5, 22 -- MIME-Version: 1.0
5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility
5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: rcmoretz-at-gmail.com
Date: Tue, 3 Feb 2009 16:13:38 -0600
Subject: [Microscopy] glutaraldehyde issue - vendor responsibility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Most times the vendors don't realize there's a problem unless a client
lets them know. I have always felt that communication with the vendor
is an essential part working in a lab. Alerting the vendor to a
potential problem helps them assist you and the rest of their customer
base. A simple or single event should not preclude your doing
business with them again, nor should it be cause to poison the rest of
the microscopy community against them.

Roger Moretz, Ph.D., retired

On Tue, Feb 3, 2009 at 4:07 PM, {dac-at-research.umass.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I don't know who the vendor is, but I be very reluctant to ever order
} from them again. It is a terrible thing to ship product when it is known
} that production issues exist. The time that people waste on compromised
} fixations and troubleshooting the problem is a huge loss and sometimes
} the loss is not recoverable. To {not} disclose the vendor leaves many
} others at risk of wasted time and lost experiments. We pay dearly for
} what should be trusted chemicals to do our work and vendors shipping bad
} product really should not be protected from exposure. They know the lot
} numbers and who received their product and yet I haven't heard that
} anyone got a notice of recall, or an email to this list to get the word
} out. We owe it to each other as fellow microscopists to keep the shades
} open and let in the light.
}
} Dale Callaham
}
}
}
} mgengle-at-email.uky.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear listers,
} }
} } I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before.
} }
} } Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems.
} }
} } So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal.
} }
} }
} } Mary Gail Engle
} } Sr Research Facility Manager
} } Electron Microscopy & Imaging Facility
} } HSRB rm 001
} } Ph (859) 323-6108
} } FAX (859) 323-8089
} } BBSRB rm o74
} } Ph (859)323-2701
} } FAX (859) 257-1581
} } University of KY
} } Lexington, KY 40536
} }
} }
} }
} }
} } ==============================Original Headers==============================
} } 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009
} } 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76])
} } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089
} } 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600
} } 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55])
} } 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500
} } 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu
} } 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500
} } 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} } 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} } 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500
} } 8, 24 -- Subject: glutaraldehyde issue
} } 8, 24 -- Thread-Topic: glutaraldehyde issue
} } 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ==
} } 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu}
} } 8, 24 -- Accept-Language: en-US
} } 8, 24 -- Content-Language: en-US
} } 8, 24 -- X-MS-Has-Attach:
} } 8, 24 -- X-MS-TNEF-Correlator:
} } 8, 24 -- acceptlanguage: en-US
} } 8, 24 -- Content-Type: text/plain; charset="us-ascii"
} } 8, 24 -- MIME-Version: 1.0
} } 8, 24 -- Content-Transfer-Encoding: 8bit
} } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089
} } ==============================End of - Headers==============================
}
} ==============================Original Headers==============================
} 5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009
} 5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
} 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913
} 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600
} 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
} 5, 22 -- (authenticated bits=0)
} 5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347
} 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500
} 5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu}
} 5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500
} 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
} 5, 22 -- Reply-To: dac-at-research.umass.edu
} 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
} 5, 22 -- MIME-Version: 1.0
} 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} 5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility
} 5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
} 5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 5, 22 -- Content-Transfer-Encoding: 7bit
} 5, 22 -- X-Whitelist: TRUE
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
4, 35 -- From rcmoretz-at-gmail.com Tue Feb 3 16:13:38 2009
4, 35 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.175])
4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13MDZU2029130
4, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:13:36 -0600
4, 35 -- Received: by wf-out-1314.google.com with SMTP id 27so2169806wfd.21
4, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 03 Feb 2009 14:13:33 -0800 (PST)
4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 35 -- d=gmail.com; s=gamma;
4, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
4, 35 -- :date:message-id:subject:from:to:content-type
4, 35 -- :content-transfer-encoding;
4, 35 -- bh=/Fowp56ZgMcuY8RW3JjhMlAi6C3YiR4+XopmyD1uzaE=;
4, 35 -- b=RcW80n4jgzhBUD3s0C93fhw4ipc02xDD0ugzcRK4+qxsNTJrb242hrg5+SJxhnGTXT
4, 35 -- BOmph5SmeYelC4zm0XC2emNxIkLwbb6vCccf4iWhDlk9fBN/kWzEdjU8zy3KAfajlzwo
4, 35 -- axQTuw3y4uKkL4J+WmGibrYWZ9vEMmBl1Vzog=
4, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 35 -- d=gmail.com; s=gamma;
4, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
4, 35 -- :content-type:content-transfer-encoding;
4, 35 -- b=MMbSN8pi4bcY6hjjBYfKPSeRm+h/wj32GpjAO2/sw803q2S2BziYUAqxu92ESAbKt2
4, 35 -- uAI8gxDG47WaMqkaNCdLOo4Swjj8eH/4Ts10DQlw3EAQIJ4uuECRGioKX1/kQno9Enoz
4, 35 -- gmR7AqiLwHAJglgSZdxRbMC4tJEHcobHAHZgQ=
4, 35 -- MIME-Version: 1.0
4, 35 -- Received: by 10.142.230.9 with SMTP id c9mr2555530wfh.101.1233699213853; Tue,
4, 35 -- 03 Feb 2009 14:13:33 -0800 (PST)
4, 35 -- In-Reply-To: {200902032107.n13L7LXP019781-at-ns.microscopy.com}
4, 35 -- References: {200902032107.n13L7LXP019781-at-ns.microscopy.com}
4, 35 -- Date: Tue, 3 Feb 2009 17:13:33 -0500
4, 35 -- Message-ID: {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com}
4, 35 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility
4, 35 -- From: Roger Moretz {rcmoretz-at-gmail.com}
4, 35 -- To: dac-at-research.umass.edu, Microscopy Listserv {Microscopy-at-microscopy.com}
4, 35 -- Content-Type: text/plain; charset=ISO-8859-1
4, 35 -- Content-Transfer-Encoding: 8bit
4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13MDZU2029130
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Tue, 3 Feb 2009 16:56:52 -0600
Subject: [Microscopy] glutaraldehyde issue - vendor responsibility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger,

I agree with you completely. I think the vendor should be the first one
contacted in the event of a problem, before the list. But where Mary
Gail said they were aware they had production problems and had still
shipped product with no warnings - hadn't let her know, or anyone else
most likely, this is a breach of trust to their consumers and clearly
has led to some compromised work and wasted time. I would lose trust in
such a vendor. I don't think it is good enough to ship product and hope
it works out and wait for the "field testers" to complain;
glutaraldehyde is a chemical and can be tested and in the case that
anything seems wrong it shouldn't be shipped to unsuspecting users. That
isn't the way to build trust.

Dale

Roger Moretz wrote:
} Most times the vendors don't realize there's a problem unless a client
} lets them know. I have always felt that communication with the vendor
} is an essential part working in a lab. Alerting the vendor to a
} potential problem helps them assist you and the rest of their customer
} base. A simple or single event should not preclude your doing
} business with them again, nor should it be cause to poison the rest of
} the microscopy community against them.
}
} Roger Moretz, Ph.D., retired
}
} On Tue, Feb 3, 2009 at 4:07 PM, {dac-at-research.umass.edu} wrote:
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I don't know who the vendor is, but I be very reluctant to ever order
} } from them again. It is a terrible thing to ship product when it is known
} } that production issues exist. The time that people waste on compromised
} } fixations and troubleshooting the problem is a huge loss and sometimes
} } the loss is not recoverable. To {not} disclose the vendor leaves many
} } others at risk of wasted time and lost experiments. We pay dearly for
} } what should be trusted chemicals to do our work and vendors shipping bad
} } product really should not be protected from exposure. They know the lot
} } numbers and who received their product and yet I haven't heard that
} } anyone got a notice of recall, or an email to this list to get the word
} } out. We owe it to each other as fellow microscopists to keep the shades
} } open and let in the light.
} }
} } Dale Callaham
} }
} }
} }
} } mgengle-at-email.uky.edu wrote:
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Dear listers,
} } }
} } } I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before.
} } }
} } } Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems.
} } }
} } } So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal.
} } }
} } }
} } } Mary Gail Engle
} } } Sr Research Facility Manager
} } } Electron Microscopy & Imaging Facility
} } } HSRB rm 001
} } } Ph (859) 323-6108
} } } FAX (859) 323-8089
} } } BBSRB rm o74
} } } Ph (859)323-2701
} } } FAX (859) 257-1581
} } } University of KY
} } } Lexington, KY 40536
} } }
} } }
} } }
} } }
} } } ==============================Original Headers==============================
} } } 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009
} } } 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76])
} } } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089
} } } 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600
} } } 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55])
} } } 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500
} } } 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu
} } } 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500
} } } 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
} } } 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} } } 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500
} } } 8, 24 -- Subject: glutaraldehyde issue
} } } 8, 24 -- Thread-Topic: glutaraldehyde issue
} } } 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ==
} } } 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu}
} } } 8, 24 -- Accept-Language: en-US
} } } 8, 24 -- Content-Language: en-US
} } } 8, 24 -- X-MS-Has-Attach:
} } } 8, 24 -- X-MS-TNEF-Correlator:
} } } 8, 24 -- acceptlanguage: en-US
} } } 8, 24 -- Content-Type: text/plain; charset="us-ascii"
} } } 8, 24 -- MIME-Version: 1.0
} } } 8, 24 -- Content-Transfer-Encoding: 8bit
} } } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089
} } } ==============================End of - Headers==============================
} } ==============================Original Headers==============================
} } 5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009
} } 5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
} } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913
} } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600
} } 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
} } 5, 22 -- (authenticated bits=0)
} } 5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347
} } 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500
} } 5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu}
} } 5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500
} } 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
} } 5, 22 -- Reply-To: dac-at-research.umass.edu
} } 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
} } 5, 22 -- MIME-Version: 1.0
} } 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} } 5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility
} } 5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
} } 5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com}
} } 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 5, 22 -- Content-Transfer-Encoding: 7bit
} } 5, 22 -- X-Whitelist: TRUE
} } ==============================End of - Headers==============================
} }

==============================Original Headers==============================
4, 22 -- From dac-at-research.umass.edu Tue Feb 3 16:56:52 2009
4, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13MuqBY011702
4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:56:52 -0600
4, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
4, 22 -- (authenticated bits=0)
4, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13Mup43030634
4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 17:56:51 -0500
4, 22 -- Message-ID: {4988CC0E.3060201-at-research.umass.edu}
4, 22 -- Date: Tue, 03 Feb 2009 17:58:22 -0500
4, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
4, 22 -- Reply-To: dac-at-research.umass.edu
4, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
4, 22 -- MIME-Version: 1.0
4, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
4, 22 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility
4, 22 -- References: {200902032107.n13L7LXP019781-at-ns.microscopy.com} {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com}
4, 22 -- In-Reply-To: {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com}
4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Tue, 3 Feb 2009 22:27:59 -0600
Subject: [Microscopy] AFM - Broken Multimode AFM Scanner wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'd like to buy a broken vertical engage scanner for the NanoScope Multimode
AFM:
--Model EV or JV scanner, broken or working, whether or not it is labeled
with a "V".

A vertical engage scanner has a single screw at its base (and built-in
springs on its sides), as shown in this photo.
http://www.asmicro.com/images/4576-EV-Vertical%20Engage%20AFM%20Scanner%20LR.JPG

In contrast, an ordinary scanner has 3 screws at its base (2 adjusted
manually, one coupled to the stepper motor) and uses separate springs to
secure the optical head.

Please contact me offline for further details.
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
5, 24 -- From donc-at-asmicro.com Tue Feb 3 22:27:59 2009
5, 24 -- Received: from smtp108.sbc.mail.re2.yahoo.com (smtp108.sbc.mail.re2.yahoo.com [68.142.229.97])
5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n144RvHP005290
5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 22:27:58 -0600
5, 24 -- Received: (qmail 17032 invoked from network); 4 Feb 2009 04:27:56 -0000
5, 24 -- Received: from unknown (HELO asm15) (donc-at-68.51.122.238 with login)
5, 24 -- by smtp108.sbc.mail.re2.yahoo.com with SMTP; 4 Feb 2009 04:27:56 -0000
5, 24 -- X-YMail-OSG: ApE4jYkVM1mmv2YSjBsMmiJ04u74fsSfLl_N4bbhSin8xjYYAUQqyow_.Our4D0R8vR4KZr6qdNFCveiwnh4Vjn4hheoIYm_ENHCTBLb7CXT02fD0AmBqp1VdXfzavlpjhAaqz.gmRVqHT56Qa8gdQyQxjvM3RnTULuS08jVvZBqD8kQc2e1waxRUGo1QBoRAcxlsxsRENsEoLgZjU5m53NA4PqrgPaoL2_oe4ZUh4zHwA--
5, 24 -- X-Yahoo-Newman-Property: ymail-3
5, 24 -- Message-ID: {A8754912B42A434BB5B7245AE2A34716-at-asm15}
5, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
5, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
5, 24 -- Subject: AFM - Broken Multimode AFM Scanner wanted
5, 24 -- Date: Tue, 3 Feb 2009 23:27:43 -0500
5, 24 -- MIME-Version: 1.0
5, 24 -- Content-Type: text/plain;
5, 24 -- format=flowed;
5, 24 -- charset="iso-8859-1";
5, 24 -- reply-type=original
5, 24 -- Content-Transfer-Encoding: 7bit
5, 24 -- X-Priority: 3
5, 24 -- X-MSMail-Priority: Normal
5, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Tue, 3 Feb 2009 23:45:30 -0600
Subject: [Microscopy] SEM, Topcon Specs.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anybody have specs (Resolution, voltage, etc...) on an old Topcon
ABT-55 and an ABT-SX-40A?

Even if you don't have published specs, I would appreciate anecdotal specs...

Thanks,

Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
5, 31 -- From kraftpiano-at-gmail.com Tue Feb 3 23:45:30 2009
5, 31 -- Received: from mail-qy0-f17.google.com (mail-qy0-f17.google.com [209.85.221.17])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n145jSMC021418
5, 31 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 23:45:29 -0600
5, 31 -- Received: by qyk10 with SMTP id 10so3339883qyk.18
5, 31 -- for {microscopy-at-microscopy.com} ; Tue, 03 Feb 2009 21:45:27 -0800 (PST)
5, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
5, 31 -- :from:to:content-type:content-transfer-encoding;
5, 31 -- bh=vMqD0pkVICNnXUK4RRnRoD5eZjr7pY4HWWkD4kUchJ4=;
5, 31 -- b=xmjH2ektr1XaUSxs70yhEiu5LtGgcH7xWCzwx/DoBRYAIRWJO0dm/hoNtXzPUnTfXJ
5, 31 -- AScisubKULwZ5F0H2cXyHngP8JRpgZRYl9cWT4ajvAbkcYCG1k3LWkWtGtKzG2u/hSoH
5, 31 -- 8kQiUB9XZucNWPlPvplAIUnaGRUdODGlesQRU=
5, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
5, 31 -- :content-transfer-encoding;
5, 31 -- b=I0TPoJSp9nvAsYZ0qx7BMtwShqx0xF7nqlfyKaa8fYYPrmMuffnxgcNVORwF1VufwI
5, 31 -- P7EDZU/wBn34GEoh2ezNhETCFduQ4MdmY4sblBGuIp7CEAT1j+mjkJU92MuUcNL7FVQX
5, 31 -- aW3ptBw1B6hj+cDKfiWSCoz6lZaz81DCU2pK4=
5, 31 -- MIME-Version: 1.0
5, 31 -- Received: by 10.214.243.18 with SMTP id q18mr9728111qah.362.1233726327249;
5, 31 -- Tue, 03 Feb 2009 21:45:27 -0800 (PST)
5, 31 -- Date: Wed, 4 Feb 2009 00:45:27 -0500
5, 31 -- Message-ID: {25e2b0d20902032145g375881fayc64e3a827c38c089-at-mail.gmail.com}
5, 31 -- Subject: SEM, Topcon Specs.
5, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
5, 31 -- To: microscopy-at-microscopy.com
5, 31 -- Content-Type: text/plain; charset=ISO-8859-1
5, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Wed, 4 Feb 2009 08:10:58 -0600
Subject: [Microscopy] glutaraldehyde issue - vendor responsibility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I really have to join Roger in this. I started in EM about 2 years
before Mary Gail Engle. While most of my work is now gastroenteric
virology, EM is still part of the job description. In all this time I
have yet to find a vendor who did not view their responsibility to the
community very seriously. In fact, the only problem I've ever seen has
to do with Kodak's decision to continue providing paper and chemistry,
but that is a corporate decision I can at least understand, if not endorse.

Don't tar and feather the vendor for one event.

Paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Feb 4 08:10:58 2009
6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n14EAwTs021341
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 08:10:58 -0600
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 -- (authenticated bits=0)
6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n14EAuAw006486;
6, 20 -- Wed, 4 Feb 2009 08:10:56 -0600 (CST)
6, 20 -- Message-ID: {4989A1F0.5030208-at-umanitoba.ca}
6, 20 -- Date: Wed, 04 Feb 2009 08:10:56 -0600
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: dac-at-research.umass.edu
6, 20 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility
6, 20 -- References: {200902032104.n13L4dRO015834-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200902032104.n13L4dRO015834-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Wed, 4 Feb 2009 11:48:39 -0600
Subject: [Microscopy] Re: [3dem] GATAN freezing machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 4, 2009, at 8:37 AM, Chen Xu wrote:

} 2. Neither one does single side blotting (nicely, if possible).


Dear Chen Xu,
Blotting one face of the grid is not easy on the Vitrobot, but
blotting from the lower edge can be done by putting a piece of filter
paper in a reverse-action tweezer and inserting it through the side
port. This method can be used whenever one-sided blotting is
necessary, and it is even better than one-sided blotting if the
specimen binds to filter paper better than it does to the surface of
the grid. I am pretty sure this technique can be used with the CP3 as
well--I only saw one at M&M, so I don't have any personal experience
with it.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Wed Feb 4 11:48:39 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n14Hmdor013415
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 11:48:39 -0600
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 94755329117
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 09:48:38 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 0E4793293EB
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 09:48:33 -0800 (PST)
6, 22 -- Message-Id: {778E5D9C-E330-4C44-AD6A-C6BBE48E841A-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {20090204163754.GA23883-at-brandeis.edu}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [3dem] GATAN freezing machine
6, 22 -- Date: Wed, 4 Feb 2009 09:48:33 -0800
6, 22 -- References: {4989A725.4020401-at-spring8.or.jp} {20090204163754.GA23883-at-brandeis.edu}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Wed, 4 Feb 2009 14:24:03 -0600
Subject: [Microscopy] viaWWW: Parallax Research is looking for reps to hire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dkloos-at-parallaxray.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: dkloos-at-parallaxray.com
Name: Don Kloos

Organization: Parallax Research, Inc.

Title-Subject: [Filtered] Parallax Research is looking for reps to hire

Question: Hello All:

I need your help. Parallax Research is seeking to hire
manufacturer's reps and distributors to handle its new EDS and WDS
x-ray microanalysis products in North America, Europe, Asia, and
other regions of the world. If you know of an individual or company
in your area that you like to deal with and can recommend them,
please let me know.

Thank you for your time.

Don Kloos
Parallax Research.
Email: dkloos-at-parallaxray.com.
Phone: 1 866 581-XRAY, or +1 714 897-9779.
www.parallaxray.com



Login Host: 24.136.64.169
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Feb 4 14:24:02 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n14KO2Ja000532
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 14:24:02 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c5afa9d3f3e1-at-[206.69.208.22]}
11, 11 -- Date: Wed, 4 Feb 2009 14:24:01 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dkloos-at-parallaxray.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Parallax Research is looking for reps to hire
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Wed, 4 Feb 2009 21:07:59 -0600
Subject: [Microscopy] Puzzling EDX results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

We were analyzing some samples of tin solder with varying but small amounts
of lead. The solder was electroplated as a film (~7-10µm) on copper. We
need to know % of lead in the samples with reasonable accuracy. The amounts
of Pb varied from about 0.5 to 3% based on information from another
quantifying technique.

We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and
then repeated this on the same area two other times under identical
conditions and immediately following the previous spectrum. Each time the
ratio of Sn to Pb varied significantly. We are at a loss to explain why
there was such a difference in the ratios from the identical area. Could
this be due to something going on due to the repeated sampling of the same
area?

Any idea as to why we had such poor reproducibility?

Thanks,
Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy






==============================Original Headers==============================
12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009
12, 30 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128])
12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1537wD4026640
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58 -0600
12, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1537wpL022381
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500
12, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8])
12, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500
12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500
12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ;
12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000
12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119
12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500
12, 30 -- Subject: Puzzling EDX results
12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu}
12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu}
12, 30 -- Thread-Topic: Puzzling EDX results
12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA==
12, 30 -- Mime-version: 1.0
12, 30 -- Content-type: text/plain;
12, 30 -- charset="ISO-8859-1"
12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC) FILETIME=[F2BAF2B0:01C9873E]
12, 30 -- X-PMX-Version: 5.4.0.320885
12, 30 -- X-PerlMx-Virus-Scanned: Yes
12, 30 -- Content-Transfer-Encoding: 8bit
12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1537wD4026640
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Wed, 4 Feb 2009 22:05:22 -0600
Subject: [Microscopy] Puzzling EDX results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby:

I had many years experience analyzing electroplated SnPb solders on Cu using
table-top micro-xrf instruments (Seiko, Fischer, CMI, Thermo, MXRF units).
It's obviously not quite the same as SEM/EDS in terms of excitation, but
here's some ideas that may help:

1) Sn Ka and Pb La intensities vary with solder thickness AND composition.
If your sample is not infinite with respect to these energies, you will get
varying results depending on where you measure (or what angle). In your
case, the layer is almost all Sn, so the film infinite thickness is about
50um or more with respect to Sn Ka.
2) If you're using Sn La and Pb Ma (or even Pb La to a lesser extent) then
the film infinite thickness is much lower. I'm not sure what lines you are
using, but you are accelerating at 30KV, enough to excite Sn Ka.
3) Solder is notoriously non-uniform in plated and hot-flow processes. I
found in studies that the composition varied with depth as well. In
addition, an intermetallic Sn-Cu layer begins forming at the Sn-Cu layer
interface which can affect results, especially for thinner layers. Are you
measuring EXACTLY the same area and getting drastically different results?
4) If it is just reproducibility, what are your net countrates for Sn and
Pb? Peak/background?
5) In SnPb/Cu on circuit boards, the presence of Br in the FR4 / G10
substrates interfered with Pb La considerably and somewhat with Pb Ma.
Consider the interference from Br.
6) How are you quantifying the composition and thickness? (They're related
unless you're measuring an 'infinitely thick' layer and/or using the lower
energy lines.)

Call me if you want to talk.

Regards,

Don

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.

Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Wednesday, February 04, 2009 7:17 PM
To: dkloos-at-parallaxray.com

Hi all,

We were analyzing some samples of tin solder with varying but small amounts
of lead. The solder was electroplated as a film (~7-10µm) on copper. We
need to know % of lead in the samples with reasonable accuracy. The amounts
of Pb varied from about 0.5 to 3% based on information from another
quantifying technique.

We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and
then repeated this on the same area two other times under identical
conditions and immediately following the previous spectrum. Each time the
ratio of Sn to Pb varied significantly. We are at a loss to explain why
there was such a difference in the ratios from the identical area. Could
this be due to something going on due to the repeated sampling of the same
area?

Any idea as to why we had such poor reproducibility?

Thanks,
Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy






==============================Original Headers==============================
12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009
12, 30 -- Received: from mailhub128.itcs.purdue.edu
(mailhub128.itcs.purdue.edu [128.210.5.128])
12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1537wD4026640
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58
-0600
12, 30 -- Received: from mailhub126.itcs.purdue.edu
(mailhub126.itcs.purdue.edu [128.210.5.126])
12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n1537wpL022381
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58
-0500
12, 30 -- Received: from 1061exfe01a.itap.purdue.edu
(1061exfe01a.itap.purdue.edu [128.210.1.8])
12, 30 -- by mailhub126.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58
-0500
12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by
1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500
12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl
([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu
([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ;
12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000
12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119
12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500
12, 30 -- Subject: Puzzling EDX results
12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu}
12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu}
12, 30 -- Thread-Topic: Puzzling EDX results
12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA==
12, 30 -- Mime-version: 1.0
12, 30 -- Content-type: text/plain;
12, 30 -- charset="ISO-8859-1"
12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC)
FILETIME=[F2BAF2B0:01C9873E]
12, 30 -- X-PMX-Version: 5.4.0.320885
12, 30 -- X-PerlMx-Virus-Scanned: Yes
12, 30 -- Content-Transfer-Encoding: 8bit
12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1537wD4026640
==============================End of - Headers==============================



==============================Original Headers==============================
26, 31 -- From dkloos-at-parallaxray.com Wed Feb 4 22:05:22 2009
26, 31 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1545LaG009350
26, 31 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:05:21 -0600
26, 31 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
26, 31 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
26, 31 -- (envelope-from {dkloos-at-parallaxray.com} )
26, 31 -- id 1LUvUa-0005QY-JR; Wed, 04 Feb 2009 21:05:32 -0700
26, 31 -- Reply-To: {dkloos-at-parallaxray.com}
26, 31 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
26, 31 -- To: {dsherman-at-purdue.edu}
26, 31 -- Cc: {microscopy-at-microscopy.com}
26, 31 -- References: {200902050316.n153GpuM007423-at-ns.microscopy.com}
26, 31 -- Subject: RE: [Microscopy] Puzzling EDX results
26, 31 -- Date: Wed, 4 Feb 2009 20:05:16 -0800
26, 31 -- Organization: Parallax Research
26, 31 -- Message-ID: {4EE6E5D005D146CF99708D3736DE3B3E-at-donl}
26, 31 -- MIME-Version: 1.0
26, 31 -- Content-Type: text/plain;
26, 31 -- charset="iso-8859-1"
26, 31 -- X-Mailer: Microsoft Office Outlook 11
26, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
26, 31 -- Thread-Index: AcmHQDcr3qoMYnyrTO2bXd1E+DCaogAA1A+g
26, 31 -- In-Reply-To: {200902050316.n153GpuM007423-at-ns.microscopy.com}
26, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
26, 31 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
26, 31 -- X-AntiAbuse: Original Domain - microscopy.com
26, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
26, 31 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
26, 31 -- Content-Transfer-Encoding: 8bit
26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1545LaG009350
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 4 Feb 2009 22:10:51 -0600
Subject: [Microscopy] Re: Puzzling EDX results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What was the morphology of the analyzed area? Spikes of
Sn will change after being whacked by a 30 KV beam.
Why did you use 30KV? For all Ma peaks, 5-6KV out to
do the job. You could double check this at 20KV for
La peaks.

The geometry of the specimen will make a difference
and so will the effects of being scanned. Did the scanned
areas show polymerization rectangles? If so, that pushes
up H, C and O. So all other elements degrade in %. Of
course not due to EDS non-detected H.

I would suggest trying 6KV, then 10KV and finally 15KV.
See what you get. Did you detect any Cu? Is the sample
surely grounded? Is the probe current near the same from
one collection to another? If not, why not? That will make
a big difference.

If I did not say so here, I have to build a spectra for
special specimens that start at 3KV and wind up at 20KV.
This also assumes that your specimen is at the EDS analytical
WD.

Can you supply a sample for off-site comparison? I'm working
on Pb and Pb-free components (mostly ICs). The RHOS thing is
somewhat nebulous. IMO, definitely not precise.

gary g.


At 07:10 PM 2/4/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
13, 22 -- From gary-at-gaugler.com Wed Feb 4 22:10:51 2009
13, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n154AocG021523
13, 22 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:10:50 -0600
13, 22 -- Message-Id: {200902050410.n154AocG021523-at-ns.microscopy.com}
13, 22 -- Received: (qmail 25760 invoked from network); 4 Feb 2009 20:11:40 -0800
13, 22 -- Received: by simscan 1.1.0 ppid: 25756, pid: 25757, t: 0.1444s
13, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
13, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
13, 22 -- by smtp1 with SMTP; 4 Feb 2009 20:11:40 -0800
13, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
13, 22 -- Date: Wed, 04 Feb 2009 20:10:45 -0800
13, 22 -- To: dsherman-at-purdue.edu
13, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
13, 22 -- Subject: Re: [Microscopy] Puzzling EDX results
13, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
13, 22 -- In-Reply-To: {200902050310.n153A4PZ029112-at-ns.microscopy.com}
13, 22 -- References: {200902050310.n153A4PZ029112-at-ns.microscopy.com}
13, 22 -- Mime-Version: 1.0
13, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
13, 22 -- Content-Transfer-Encoding: 8bit
13, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n154AocG021523
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Wed, 4 Feb 2009 23:10:09 -0600
Subject: [Microscopy] Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

So, I thought I sent the following message to the list, but it never
showed up. I looked at again and wondered if I should resend it, at
first I wasn't going to do it, but then I decided, why not. After
reviewing it I thought it might be a little naive, but I am curious
about what you think.

Here it is:

Greetings

At the risk of being labeled a heretic, I'd like to know the current
state of biological SEM.

Here's the deal, I am teaching a class on bio SEM and just sat down to
prepare a lecture on modern biological SEM. I have lots of nice books
with cool SEM pictures, but I noticed they are all about 20 years old.
Heck, I even did some nice bio SEM, but that was, well, 20 years ago.

I tried to cruise the web to see if I could get up to date on what's
happening, but web access to journals from here is pretty lame. So is
the offering of ultrastructural type journals at our library, like,
there aren't any.

I do get a few journals and I can access a few on the web, but most of
the papers in the searchable index from JCB etc. that have SEM are,
well, pretty old. Is there much modern research using SEM being done
in biology these days? Mostly I see the SEM being applied to materials
type research. Not that the biology work that has been done is not
elegant, it just seems like a lot of it is finding its way on to
journal covers or 'coffee table' picture books after being colorized
in Photoshop.

I am OK with that, and if the kind of SEM that I know, simple fix,
dehydr, and CPD is the state of the art, then I will feel better
getting my students to do these things. But if there are sources for
something new, I would like to be able to tell them about it. I mean,
after all,how many fly eyes, bee's knees, and pollen grains can you
look at?

Out on a limb, and with my asbestos suit fully zipped,

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009
14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n155A6nj005730
14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 -0600
14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
14, 42 -- by localhost (Email Security Appliance) with SMTP id 221DF1BA6DD_98A6F4BB
14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 (GMT)
14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
14, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 177F01BA6D5_98A6F4BF
14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 (GMT)
14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
14, 42 -- with ESMTP id 45413886 for microscopy-at-microscopy.com; Wed, 04 Feb 2009 21:10:01 -0800
14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
14, 42 -- ESMTP id KEKTM900.61V for {microscopy-at-microscopy.com} ; Wed, 4
14, 42 -- Feb 2009 20:54:09 -0800
14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 648B68F74E48
14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:01 -0800 (PST)
14, 42 -- X-Virus-Scanned: amavisd-new at
14, 42 -- X-Spam-Flag: NO
14, 42 -- X-Spam-Score: -2.499
14, 42 -- X-Spam-Level:
14, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000,
14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
14, 42 -- with ESMTP id VKwKzh5c2Zca for {microscopy-at-microscopy.com} ;
14, 42 -- Wed, 4 Feb 2009 21:10:01 -0800 (PST)
14, 42 -- Received: from [172.20.3.16] (unknown [172.20.3.16])
14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 007508F74E1E
14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:00 -0800 (PST)
14, 42 -- Message-Id: {A7C0E8F7-7669-4A7A-A038-0F6A54694846-at-deltacollege.edu}
14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
14, 42 -- To: microscopy-at-microscopy.com
14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
14, 42 -- Content-Transfer-Encoding: 7bit
14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
14, 42 -- Subject: Current biological SEM
14, 42 -- Date: Wed, 4 Feb 2009 21:10:00 -0800
14, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Thu, 5 Feb 2009 07:17:29 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jonathan,
You ask a relevant question. As a biologist who has gotten
deeply into SEM in the last decade, I am surprised that there is not
more use on our side of the fence. I suspect that most of the recent
advances in SEM tech have been driven my materials folks and that the
biologists simply don't know about them. Let me give you my
perspective on where I think the advances are.

1. Environmental SEM. It is now possible to examine a fully hydrated
specimen in the SEM. The sample needs to be frozen or at least cold
to minimize evaporation but otherwise not processed at all. I am
aware of people who use ESEM to study root hairs and also floral
meristems. I suspect animal scientists use also. The point is that
fixation crtical point dry and coating are all avoided and instead
the sample is directly viewed. The drawback is that once the sample
is out of the machine it cannot be viewed again later. And the
resolution of an ESEM is no better than a conventional tungsten
filament model.

2. Wet chambers. A rather recent (5 years or so?) development are
thin membranes that the beam can pass but gas cannot. This allows
fully hydrated samples to be viewed at ambient temp and pressure in
chambers built with the top surface being that special membrane. Thus
living cells can be grown on the membrane (inside the membrane) and
viewed in the SEM while they are alive. It is of course a question
how much radiation damage the beam will induce. Still the cells are
certanly viable for a while. I have seen papers using this tech on
animal tissue culture cells. There was quite a lot of excitement when
these chambers hit the market but I don't know how successful they
have proven in the "real" world.

The above points are geared for eliminating fixation and looking at
samples as close to living as possible. This is clearly difficult
given the fundamental high-vacuum nature of the SEM beast. The other
direction where advances lay is in the high vac, high resolution end
of things. Here the advances in SEM design have been phenomenal. The
field emission gun allows resolution to be obtained that is almost as
good as the best TEMs and certainly far beyond what the good old fly
eye SEM was capable of seeing.

3. High resolution. With the field emission gun, macromolecules can
be resolved. I among others have taken advantage of this to study the
ultrastructure of the plant cell wall. Cellulose microfibrils are on
order of 10 nm and these can be readily imaged with FESEM. What is
particularly useful for me is that I can put my plant stem 1 mm by 10
mm in to instrument, get good low mag survey views and crank up the
magnficiation to the ultrastructual level in any cell on the sample
that is appropriate. The easy moving between low and high
magnifications is extremely helpful where extensive sampling is
needed or when rare features need to be found. Other biological
systems that have been profitably studied with FESEM include the
cytoskeleton, bacterial biofilms, biomineralization, and nuclear
pore complexes. All of these cases involve complex three dimensional
structures and the FESEM lets you image them at high resolution in
the intact (or semi-intact) state.

4. Low voltage. Along with the sharper probe of the FESEM comes the
ability to work at really low accelerating voltage. In the recent
vintage FESEM's it is easy to image at 1 kV and possible to go down
even as low as a tenth of that. This has several advantages. First,
many samples are destroyed or damaged at 30 kV or even at 10 kV.
Second, the lower the beam voltage the more exclusively the image
comes from the surface. This increases the resolution in the depth
direction.

Both low voltage and the smaller probe size allow much thinner metal
coats to be applied, and in some cases no coat at all. For the work I
have been doing on the cell wall, I use ca. 1 to 2 nm of Pt. This is
an order of magnitude less than typical gold coats for conventional
tungsten SEM work.

5. Backscattered imaging. Of particular importance to biologists is
being able to image backscattered electrons. This allows the
localization of gold probes, as in gold conjugated secondary
antibodies. With the latest instrumentation, one can colect electrons
from selected energy (or angle) levels and mix signals from several
detectors. Thus, the SE detector can show the topography of the
sample while the BSE detector can pick up the signal from the gold
and the two are overlain seamlessly. The sensitivity of the detectors
is such that the gold can be imaged at high contrast even with a
sample lightly covered with platinum. I took advantage of this (with
slightly older not quite so performant technology) to detect gamma
tubulin in the plant cell cortex. Others have localized antigens on
the plasma membrane and cell wall, to name a few.

There have been parallel developments in elemental analysis (EDS and
related modes) and I expect biologists have taken advantage of these
too but it is not my area so I don't know. Perhaps a helpful netizen
will chime in.

Summarizing, you are right, recent use of SEM in biology is a little
bit burried. However these uses will be well worth your digging up.
Good luck with your course,
Tobias

}
}
} Greetings
}
} At the risk of being labeled a heretic, I'd like to know the current
} state of biological SEM.
}
} Here's the deal, I am teaching a class on bio SEM and just sat down to
} prepare a lecture on modern biological SEM. I have lots of nice books
} with cool SEM pictures, but I noticed they are all about 20 years old.
} Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
}
} I tried to cruise the web to see if I could get up to date on what's
} happening, but web access to journals from here is pretty lame. So is
} the offering of ultrastructural type journals at our library, like,
} there aren't any.
}
} I do get a few journals and I can access a few on the web, but most of
} the papers in the searchable index from JCB etc. that have SEM are,
} well, pretty old. Is there much modern research using SEM being done
} in biology these days? Mostly I see the SEM being applied to materials
} type research. Not that the biology work that has been done is not
} elegant, it just seems like a lot of it is finding its way on to
} journal covers or 'coffee table' picture books after being colorized
} in Photoshop.
}
} I am OK with that, and if the kind of SEM that I know, simple fix,
} dehydr, and CPD is the state of the art, then I will feel better
} getting my students to do these things. But if there are sources for
} something new, I would like to be able to tell them about it. I mean,
} after all,how many fly eyes, bee's knees, and pollen grains can you
} look at?
}
} Out on a limb, and with my asbestos suit fully zipped,
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
12, 21 -- From baskin-at-bio.umass.edu Thu Feb 5 02:05:58 2009
12, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1585wa3024378
12, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 02:05:58 -0600
12, 21 -- Received: from [133.48.48.239] (dh48-239.nibb.ac.jp [133.48.48.239])
12, 21 -- (authenticated bits=0)
12, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n1585pIE020660
12, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
12, 21 -- Thu, 5 Feb 2009 03:05:54 -0500 (EST)
12, 21 -- Mime-Version: 1.0
12, 21 -- Message-Id: {p06240538c5b0451e1d83-at-[133.48.48.239]}
12, 21 -- In-Reply-To: {200902050510.n155AuFl006549-at-ns.microscopy.com}
12, 21 -- References: {200902050510.n155AuFl006549-at-ns.microscopy.com}
12, 21 -- Date: Thu, 5 Feb 2009 17:05:50 +0900
12, 21 -- To: jkrupp-at-deltacollege.edu
12, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
12, 21 -- Subject: Re: [Microscopy] Current biological SEM
12, 21 -- Cc: microscopy-at-microscopy.com
12, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
12, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Thu, 05 Feb 2009 03:05:56 -0500 (EST)
12, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================

From felo-at-chello.pl Thu Feb 5 05:22:56 2009
Return-Path: {felo-at-chello.pl}
Received: from google.com (dhcp-077-250-106-149.chello.nl [77.250.106.149])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15BMtEa030198
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 5 Feb 2009 05:22:55 -0600
Received: from [118.162.44.132] (HELO google.com)
by luckybuyadrink.com; Thu, 5 Feb 2009 12:20:53 +0530

Jon,

A quick answer:
"Biological Low-Voltage Scanning Electron Microscopy", H. Schatten
and j. Pawley, eds. Springer, 2008.
Lots of good information in there.
The major advances in Biological SEM have been in cryo methods and
high resolution (which pretty much means low voltage) methds. SEM
using gold-conjugated antibodies and other ligands are being done.
There are also environmental SEM and fluid-chamber SEM using
backscattered detectors.
Correlation studies of e.g. gold-conjugated primary antibodies
followed by fluorescently labeled secondaries should (I hope) become
more common.
I suspect most of the advances are in the primary literature, but not
necessarily in the microscopy journals. Rather, in journals like
"Immunity", etc.
Then again, one reason old texts are still good is that there isn't
necessarily a need for new methods, "just" new studies.

Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
5, 25 -- From oshel1pe-at-cmich.edu Thu Feb 5 07:17:29 2009
5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15DHTsk001733
5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 07:17:29 -0600
5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n15DHNNo013319
5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:17:25 -0500
5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 25 -- Thu, 5 Feb 2009 08:16:54 -0500
5, 25 -- Mime-Version: 1.0
5, 25 -- Message-Id: {f06240800c5b0957767e7-at-[141.209.160.249]}
5, 25 -- In-Reply-To: {200902050513.n155DH5n011616-at-ns.microscopy.com}
5, 25 -- References: {200902050513.n155DH5n011616-at-ns.microscopy.com}
5, 25 -- Date: Thu, 5 Feb 2009 08:16:51 -0500
5, 25 -- To: Microscopy-at-microscopy.com
5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 25 -- Subject: Re: [Microscopy] Current biological SEM
5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 25 -- X-OriginalArrivalTime: 05 Feb 2009 13:16:54.0390 (UTC) FILETIME=[03B56960:01C98794]
5, 25 -- X-Canit-CHI2: 0.00
5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
5, 25 -- X-CanItPRO-Stream: default
5, 25 -- X-Canit-Stats-ID: 8474343 - 8f4ddedec40c
5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: delannoy-at-jhmi.edu
Date: Thu, 5 Feb 2009 08:19:51 -0600
Subject: [Microscopy] Hepes OR Pipes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listservers,
What concentration of Hepes or Pipes would you use for buffering a standard TEM fixation (and subsequent washes), 0.1M, 50mM or 20 mM?
Thanks for all responses.

Michael Delannoy

==============================Original Headers==============================
2, 27 -- From delannoy-at-jhmi.edu Thu Feb 5 08:19:50 2009
2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EJmkX017907
2, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:19:50 -0600
2, 27 -- X-IronPort-AV: E=Sophos;i="4.37,385,1231131600";
2, 27 -- d="scan'208";a="186662157"
2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Feb 2009 09:20:03 -0500
2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by jesmail.johnshopkins.edu
2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006))
2, 27 -- with ESMTP id {0KEL00JV0JTEKC10-at-jesmail.johnshopkins.edu} for
2, 27 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:20:02 -0500 (EST)
2, 27 -- Received: from [10.181.192.191] (Forwarded-For: [10.16.66.59])
2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Thu, 05 Feb 2009 09:20:02 -0500
2, 27 -- Date: Thu, 05 Feb 2009 09:20:02 -0500
2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
2, 27 -- Subject: Hepes OR Pipes
2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 27 -- Message-id: {fc16d6674f2c.498aaf42-at-johnshopkins.edu}
2, 27 -- MIME-version: 1.0
2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006)
2, 27 -- Content-type: text/plain; charset=us-ascii
2, 27 -- Content-language: en
2, 27 -- Content-transfer-encoding: 7BIT
2, 27 -- Content-disposition: inline
2, 27 -- X-Accept-Language: en
2, 27 -- Priority: normal
==============================End of - Headers==============================




From: lherault-at-bu.edu
Date: Thu, 5 Feb 2009 08:42:36 -0600
Subject: [Microscopy] Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our lab deals with Dental biomaterials and we have an SEM. The biological
part comes into play for tissue engineering, e.g. seeing if cells are in
scaffolds, are they happy with the surface they are on, etc. We've also
looked at bacteria in microleakage studies. Some of these problems are
unsolved but improved upon in 20 years. From what I've seen of research,
the more diverse the background you have, the more apt you are to come up
with a better solution to a problem. Remember Edison, the inventor of the
phonograph and practical light bulb? He had to come up with a complete
system to make the light bulb work outside of the lab. He studied how the
gas company distributed gas, an already tried and true system. And the
woman who invented the circular saw blade did so because she was using a
spinning wheel while the males of her family were pushing and pulling a saw
through wood. It's connections of old and new.

Ron L

-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Thursday, February 05, 2009 12:13 AM
To: lherault-at-bu.edu

So, I thought I sent the following message to the list, but it never
showed up. I looked at again and wondered if I should resend it, at
first I wasn't going to do it, but then I decided, why not. After
reviewing it I thought it might be a little naive, but I am curious
about what you think.

Here it is:

Greetings

At the risk of being labeled a heretic, I'd like to know the current
state of biological SEM.

Here's the deal, I am teaching a class on bio SEM and just sat down to
prepare a lecture on modern biological SEM. I have lots of nice books
with cool SEM pictures, but I noticed they are all about 20 years old.
Heck, I even did some nice bio SEM, but that was, well, 20 years ago.

I tried to cruise the web to see if I could get up to date on what's
happening, but web access to journals from here is pretty lame. So is
the offering of ultrastructural type journals at our library, like,
there aren't any.

I do get a few journals and I can access a few on the web, but most of
the papers in the searchable index from JCB etc. that have SEM are,
well, pretty old. Is there much modern research using SEM being done
in biology these days? Mostly I see the SEM being applied to materials
type research. Not that the biology work that has been done is not
elegant, it just seems like a lot of it is finding its way on to
journal covers or 'coffee table' picture books after being colorized
in Photoshop.

I am OK with that, and if the kind of SEM that I know, simple fix,
dehydr, and CPD is the state of the art, then I will feel better
getting my students to do these things. But if there are sources for
something new, I would like to be able to tell them about it. I mean,
after all,how many fly eyes, bee's knees, and pollen grains can you
look at?

Out on a limb, and with my asbestos suit fully zipped,

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009
14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu
[207.62.178.150])
14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n155A6nj005730
14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07
-0600


==============================Original Headers==============================
22, 22 -- From lherault-at-bu.edu Thu Feb 5 08:42:34 2009
22, 22 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EgX5d032184
22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:42:34 -0600
22, 22 -- X-Envelope-From: lherault-at-bu.edu
22, 22 -- Received: from BUSDM801203 ([155.41.214.83])
22, 22 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n15EgOoE002292
22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:42:24 -0500
22, 22 -- From: "Ron L" {lherault-at-bu.edu}
22, 22 -- To: {microscopy-at-microscopy.com}
22, 22 -- References: {200902050512.n155Cjea010347-at-ns.microscopy.com}
22, 22 -- Subject: RE: [Microscopy] Current biological SEM
22, 22 -- Date: Thu, 5 Feb 2009 09:42:22 -0500
22, 22 -- Message-ID: {002401c9879f$f49d5030$53d6299b-at-ad.bu.edu}
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain;
22, 22 -- charset="us-ascii"
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Mailer: Microsoft Office Outlook 11
22, 22 -- In-Reply-To: {200902050512.n155Cjea010347-at-ns.microscopy.com}
22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
22, 22 -- Thread-index: AcmHUGgTbUE/6JKoSnSCkEhcCgwrDgATlNQg
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Thu, 5 Feb 2009 08:47:43 -0600
Subject: [Microscopy] Hepes OR Pipes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use 30 mM HEPES, 70 mM NaCl, 1 mM CaCl2, pH 7.4 which is roughly the
same osmolarity as half-strength Karnovsky's. I have checked the pH
after fixation and it seems stable. Tom



-----Original Message-----
X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu]
Sent: Thursday, February 05, 2009 8:22 AM
To: Phillips, Thomas E.

Listservers,
What concentration of Hepes or Pipes would you use for buffering a
standard TEM fixation (and subsequent washes), 0.1M, 50mM or 20 mM?
Thanks for all responses.

Michael Delannoy

==============================Original
Headers==============================
2, 27 -- From delannoy-at-jhmi.edu Thu Feb 5 08:19:50 2009
2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu
[162.129.8.141])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n15EJmkX017907
2, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009
08:19:50 -0600
2, 27 -- X-IronPort-AV: E=Sophos;i="4.37,385,1231131600";
2, 27 -- d="scan'208";a="186662157"
2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Feb 2009
09:20:03 -0500
2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by
jesmail.johnshopkins.edu
2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5
2006))
2, 27 -- with ESMTP id {0KEL00JV0JTEKC10-at-jesmail.johnshopkins.edu} for
2, 27 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:20:02 -0500
(EST)
2, 27 -- Received: from [10.181.192.191] (Forwarded-For: [10.16.66.59])
2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Thu, 05 Feb 2009
09:20:02 -0500
2, 27 -- Date: Thu, 05 Feb 2009 09:20:02 -0500
2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
2, 27 -- Subject: Hepes OR Pipes
2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 27 -- Message-id: {fc16d6674f2c.498aaf42-at-johnshopkins.edu}
2, 27 -- MIME-version: 1.0
2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built
May 24 2006)
2, 27 -- Content-type: text/plain; charset=us-ascii
2, 27 -- Content-language: en
2, 27 -- Content-transfer-encoding: 7BIT
2, 27 -- Content-disposition: inline
2, 27 -- X-Accept-Language: en
2, 27 -- Priority: normal
==============================End of -
Headers==============================


==============================Original Headers==============================
11, 29 -- From PhillipsT-at-missouri.edu Thu Feb 5 08:47:42 2009
11, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EldYo004448
11, 29 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:47:40 -0600
11, 29 -- X-IronPort-Anti-Spam-Filtered: true
11, 29 -- X-IronPort-Anti-Spam-Result: ApoEAO6KiknRauUp/2dsb2JhbADDP4UziEeEFgaCM4Mi
11, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
11, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Feb 2009 08:48:30 -0600
11, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
11, 29 -- Thu, 5 Feb 2009 08:48:30 -0600
11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 29 -- Content-class: urn:content-classes:message
11, 29 -- MIME-Version: 1.0
11, 29 -- Content-Type: text/plain;
11, 29 -- charset="US-ASCII"
11, 29 -- Subject: RE: [Microscopy] Hepes OR Pipes
11, 29 -- Date: Thu, 5 Feb 2009 08:48:32 -0600
11, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05DF21EA-at-UM-XMAIL06.um.umsystem.edu}
11, 29 -- In-Reply-To: {200902051422.n15EMQqQ019086-at-ns.microscopy.com}
11, 29 -- X-MS-Has-Attach:
11, 29 -- X-MS-TNEF-Correlator:
11, 29 -- Thread-Topic: [Microscopy] Hepes OR Pipes
11, 29 -- Thread-Index: AcmHnTq5/3MiWONmQma9rYEPvbHj0AAA3WQA
11, 29 -- References: {200902051422.n15EMQqQ019086-at-ns.microscopy.com}
11, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
11, 29 -- To: {delannoy-at-jhmi.edu} , {microscopy-at-microscopy.com}
11, 29 -- X-OriginalArrivalTime: 05 Feb 2009 14:48:30.0235 (UTC) FILETIME=[CF7CCEB0:01C987A0]
11, 29 -- Content-Transfer-Encoding: 8bit
11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15EldYo004448
==============================End of - Headers==============================




From: gas19-at-chrysler.com
Date: Thu, 5 Feb 2009 08:59:52 -0600
Subject: [Microscopy] viaWWW: Puzzling EDX results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gas19-at-chrysler.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gas19-at-chrysler.com
Name: Gerald Shulke

Organization: Chrysler LLC

Title-Subject: [Microscopy] Puzzling EDX results

Question: Debby,

Was there any drift to the SEM image during the analysis? My
assumption is that there was a slight drift in the signal over the
120 second collection time, and you moved to a slightly different
chemistry in the sample. The solder probably does not have a very
homogenous microstructure, so your image shifted to a different
microstructural feature. Check to make sure your sample is
adequately grounded and maybe select a larger area to collect the
spectrum from, so there is more of an averaging of all of the
microstructural constituents. Most likely there are small globules
of lead, so you're drifting over these regions during the analysis.
BSE should show the segregations.

Gerald Shulke

Login Host: 129.9.163.233
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Feb 5 08:59:51 2009
8, 11 -- Received: from [206.69.208.26] ([206.69.208.26])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Exowd023992
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:59:51 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c5b0afab3b63-at-[146.139.72.120]}
8, 11 -- Date: Thu, 5 Feb 2009 09:01:24 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: gas19-at-chrysler.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Puzzling EDX results
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: j.janssen-at-nki.nl
Date: Thu, 5 Feb 2009 10:01:09 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.janssen-at-nki.nl
Name: Hans Janssen

Organization: Netherlands Cancer Institute

Title-Subject: [Filtered] How to get rid of a magnetic field

Question: We want to move our EMís to a building
close to tram tracks. At pre-installation
measurements by FEI a distorting field of 10
mGauss (4.4 mGauss is the limit for a Tecnai12)
was found. This building was a former MRI
location; in the walls are steel (2cm thick)
plating and a copper Faraday cage both with large
holes in the ceiling. Can you give me advice on
preventing this magnetic field? Is restoring the
Faraday cage and/or the steel cage enough? Or do
we need a mu-ferro or a Helmholtz cage? What will
be the approximate costs of these?

Best regards, Hans


Login Host: 194.171.7.39
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009
9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832
9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600
9, 13 -- Mime-Version: 1.0
9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]}
9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600
9, 13 -- To: microscopy-at-microscopy.com
9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver)
9, 13 -- Subject: viaWWW: How to get rid of a magnetic field
9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 13 -- Content-Transfer-Encoding: 8bit
9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832
==============================End of - Headers==============================




From: vlynch-at-mail.wsu.edu
Date: Thu, 5 Feb 2009 10:05:33 -0600
Subject: [Microscopy] Philips TEM CM-200 Manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Does anyone have a manual for a Philips CM-200 FEG-TEM? We recently
moved this TEM to our facility and it is now operational again, but
the service rep says he cannot find a manual or any documentation and
that the company just recently threw out all of the copies they had.
If you have a manual could we get a copy?
Sincerly,
Valerie
--
Valerie Lynch-Holm, Ph.D.
Franceschi Microscopy & Imaging Center
133 Abelson Hall
Washington State University
Pullman, Wa 99164-4210
509-335-3025

==============================Original Headers==============================
1, 20 -- From vlynch-at-mail.wsu.edu Thu Feb 5 10:05:32 2009
1, 20 -- Received: from mx.wsu.edu.pph5a.pphosted.com (mx.wsu.edu.pph5a.pphosted.com [208.86.201.34])
1, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G5VxN015328
1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:05:32 -0600
1, 20 -- Received: from jaguar.it.wsu.edu (jaguar.it.wsu.edu [134.121.0.73])
1, 20 -- by mx.wsu.edu.pph5a.pphosted.com (8.14.1/8.14.1) with ESMTP id n15G5gkN026596
1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:05:43 -0800
1, 20 -- Received: from [134.121.42.104] (vrf.emc.wsu.edu [134.121.42.104])
1, 20 -- by jaguar.it.wsu.edu (8.12.11.20060308/8.12.11) with ESMTP id n15G5glJ010027
1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:05:42 -0800
1, 20 -- Mime-Version: 1.0
1, 20 -- Message-Id: {a06200700c5b0bd9eaa7e-at-[134.121.42.104]}
1, 20 -- Date: Thu, 5 Feb 2009 08:05:41 -0800
1, 20 -- To: Microscopy-at-microscopy.com
1, 20 -- From: Valerie Lynch-Holm {vlynch-at-mail.wsu.edu}
1, 20 -- Subject: Philips TEM CM-200 Manual?
1, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
1, 20 -- X-Virus-Scanned: by amavisd-milter (http://amavis.org/)
1, 20 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-05_03:2009-02-05,2009-02-05,2009-02-05 signatures=0
1, 20 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0902050088
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Thu, 5 Feb 2009 10:30:51 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon,
I agree. Biological SEM has changed and materials applications are more
plentiful. However, in my opinion and experience, there is still room for
the "old ways". Delta has a wonderful library of old SEM proceedings and
there are many good papers in them. (Let's hope that "old" doesn't mean
"obsolete" or we'd all be in trouble.). It does seems that environmental,
low vacuum and high resolution SEM are most relevant nowadays, however, many
biological labs don't have the luxury of owning them.
As a technician who is responsible for SEM processing and training on a
standard SEM, there is still a call for different biological preps. Your
students would benefit from learning how to process cells (suspensions or
grown on filters), OTO, cryo fracture, maceration, backscatter, replicas
etc. Basic theory and hands on are crucial.
When I was a student, we used the book "Preparation of Biological Specimens
for Scanning Electron Microscopy" published by Scanning Electron Microscopy,
Inc. and compiled by Judy Murphy and Godfried M. Roomans. It's from 1984 but
it still has allot of ideas for your class. I'm not sure if it's still in
print but there used to be one there at Delta. The more protocols they
learn, the better equipped they will be when they graduate.
Pat Kysar
University of California, Davis
Medical School, Pathology
EM Lab

----- Original Message -----
X-from: {baskin-at-bio.umass.edu}
To: {pekysar-at-ucdavis.edu}
Sent: Thursday, February 05, 2009 12:15 AM

Jon,

The type of microscopy has to fit the question. SEM can be a very valuable
biological tool if the question is appropriate. For instance, we do a lot
of cryoSEM so that we do not have the artifacts (shrinkage, etc) that go
with critical point drying.

I have one investigator who is interested in chemical crystal formation in
plants. She does cryo and fractures the plant tissue to reveal the crystals
in their native state without any possibility of extraction during aqueous
fixation, etc.

Other uses revolve around food processing, biomedical products, and
pharmaceutical applications with samples such as starch, collagen, and
synthetic hydrogels. Documenting bacteria growth on food products before
and after treatments to eliminate the bacteria would be meaningless if you
had to process the sample as that would likely also remove bacteria.
CryoSEM would preserve them on the sample.

Some samples can be done with traditional critical point drying even though
there may be some distortion. An example would be to monitor cell growth on
various substrates. Cells would be expected to shrink but their overall
distribution would stay the same. Many plant tissues do well with CPD but
others (very young plants) require cryoSEM.

And the list goes on for applications to biological (or hydrated samples in
general...)

Debby

--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: {jkrupp-at-deltacollege.edu}
} Reply-To: {jkrupp-at-deltacollege.edu}
} Date: Wed, 4 Feb 2009 23:12:05 -0600
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] Current biological SEM
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} So, I thought I sent the following message to the list, but it never
} showed up. I looked at again and wondered if I should resend it, at
} first I wasn't going to do it, but then I decided, why not. After
} reviewing it I thought it might be a little naive, but I am curious
} about what you think.
}
} Here it is:
}
} Greetings
}
} At the risk of being labeled a heretic, I'd like to know the current
} state of biological SEM.
}
} Here's the deal, I am teaching a class on bio SEM and just sat down to
} prepare a lecture on modern biological SEM. I have lots of nice books
} with cool SEM pictures, but I noticed they are all about 20 years old.
} Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
}
} I tried to cruise the web to see if I could get up to date on what's
} happening, but web access to journals from here is pretty lame. So is
} the offering of ultrastructural type journals at our library, like,
} there aren't any.
}
} I do get a few journals and I can access a few on the web, but most of
} the papers in the searchable index from JCB etc. that have SEM are,
} well, pretty old. Is there much modern research using SEM being done
} in biology these days? Mostly I see the SEM being applied to materials
} type research. Not that the biology work that has been done is not
} elegant, it just seems like a lot of it is finding its way on to
} journal covers or 'coffee table' picture books after being colorized
} in Photoshop.
}
} I am OK with that, and if the kind of SEM that I know, simple fix,
} dehydr, and CPD is the state of the art, then I will feel better
} getting my students to do these things. But if there are sources for
} something new, I would like to be able to tell them about it. I mean,
} after all,how many fly eyes, bee's knees, and pollen grains can you
} look at?
}
} Out on a limb, and with my asbestos suit fully zipped,
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original Headers==============================
} 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009
} 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu
} [207.62.178.150])
} 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n155A6nj005730
} 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 -0600
} 14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain
} [127.0.0.1])
} 14, 42 -- by localhost (Email Security Appliance) with SMTP id
} 221DF1BA6DD_98A6F4BB
} 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000
} (GMT)
} 14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us
} [207.62.178.236])
} 14, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id
} 177F01BA6D5_98A6F4BF
} 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000
} (GMT)
} 14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} 14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 14, 42 -- with ESMTP id 45413886 for microscopy-at-microscopy.com; Wed, 04 Feb
} 2009 21:10:01 -0800
} 14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} 14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15)
} with
} 14, 42 -- ESMTP id KEKTM900.61V for {microscopy-at-microscopy.com} ;
} Wed, 4
} 14, 42 -- Feb 2009 20:54:09 -0800
} 14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 648B68F74E48
} 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:01 -0800
} (PST)
} 14, 42 -- X-Virus-Scanned: amavisd-new at
} 14, 42 -- X-Spam-Flag: NO
} 14, 42 -- X-Spam-Score: -2.499
} 14, 42 -- X-Spam-Level:
} 14, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
} tests=[AWL=0.000,
} 14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
} 14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new,
} port 10024)
} 14, 42 -- with ESMTP id VKwKzh5c2Zca for {microscopy-at-microscopy.com} ;
} 14, 42 -- Wed, 4 Feb 2009 21:10:01 -0800 (PST)
} 14, 42 -- Received: from [172.20.3.16] (unknown [172.20.3.16])
} 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 007508F74E1E
} 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:00 -0800
} (PST)
} 14, 42 -- Message-Id: {A7C0E8F7-7669-4A7A-A038-0F6A54694846-at-deltacollege.edu}
} 14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} 14, 42 -- To: microscopy-at-microscopy.com
} 14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 14, 42 -- Content-Transfer-Encoding: 7bit
} 14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 14, 42 -- Subject: Current biological SEM
} 14, 42 -- Date: Wed, 4 Feb 2009 21:10:00 -0800
} 14, 42 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================


==============================Original Headers==============================
11, 31 -- From dsherman-at-purdue.edu Thu Feb 5 10:30:49 2009
11, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130])
11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15GUj2v008005
11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:30:48 -0600
11, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
11, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n15GVLJB009976
11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:31:21 -0500
11, 31 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10])
11, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n15GVLGp025477
11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:31:21 -0500
11, 31 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
11, 31 -- Thu, 5 Feb 2009 11:31:21 -0500
11, 31 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ;
11, 31 -- Thu, 5 Feb 2009 16:30:26 +0000
11, 31 -- User-Agent: Microsoft-Entourage/12.15.0.081119
11, 31 -- Date: Thu, 05 Feb 2009 11:30:20 -0500
11, 31 -- Subject: Re: [Microscopy] Current biological SEM
11, 31 -- From: Debby Sherman {dsherman-at-purdue.edu}
11, 31 -- To: {jkrupp-at-deltacollege.edu} ,
11, 31 -- "message to: MSA list" {microscopy-at-microscopy.com}
11, 31 -- Message-ID: {C5B07E4C.3A89D%dsherman-at-exchange.purdue.edu}
11, 31 -- Thread-Topic: [Microscopy] Current biological SEM
11, 31 -- Thread-Index: AcmHrwkwaSMlGsvNS0+x3tSPJp4weg==
11, 31 -- In-Reply-To: {200902050512.n155C5DG008911-at-ns.microscopy.com}
11, 31 -- Mime-version: 1.0
11, 31 -- Content-type: text/plain;
11, 31 -- charset="US-ASCII"
11, 31 -- Content-transfer-encoding: 7bit
11, 31 -- X-OriginalArrivalTime: 05 Feb 2009 16:31:21.0084 (UTC) FILETIME=[2D99A3C0:01C987AF]
11, 31 -- X-PMX-Version: 5.4.0.320885
11, 31 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 5 Feb 2009 10:35:45 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hans,
I can't answer your questions about the field and shielding, but it strikes
me that you're also going to have a serious problem with vibration from the
trams and it may trump the field problem.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl]
Sent: Thursday, February 05, 2009 11:07 AM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.janssen-at-nki.nl
Name: Hans Janssen

Organization: Netherlands Cancer Institute

Title-Subject: [Filtered] How to get rid of a magnetic field

Question: We want to move our EMís to a building
close to tram tracks. At pre-installation
measurements by FEI a distorting field of 10
mGauss (4.4 mGauss is the limit for a Tecnai12)
was found. This building was a former MRI
location; in the walls are steel (2cm thick)
plating and a copper Faraday cage both with large
holes in the ceiling. Can you give me advice on
preventing this magnetic field? Is restoring the
Faraday cage and/or the steel cage enough? Or do
we need a mu-ferro or a Helmholtz cage? What will
be the approximate costs of these?

Best regards, Hans


Login Host: 194.171.7.39
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009
9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n15G17MS011832
9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09
-0600
9, 13 -- Mime-Version: 1.0
9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]}
9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600
9, 13 -- To: microscopy-at-microscopy.com
9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver)
9, 13 -- Subject: viaWWW: How to get rid of a magnetic field
9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 13 -- Content-Transfer-Encoding: 8bit
9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n15G17MS011832
==============================End of - Headers==============================




==============================Original Headers==============================
21, 25 -- From kenconverse-at-qualityimages.biz Thu Feb 5 10:35:30 2009
21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15GZPeJ011759
21, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:35:27 -0600
21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
21, 25 -- with ESMTP
21, 25 -- id {20090205163620.HKZI23506.cdptpa-omta04.mail.rr.com-at-Ken} ;
21, 25 -- Thu, 5 Feb 2009 16:36:20 +0000
21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 25 -- To: {j.janssen-at-nki.nl} , "MSA Listserver" {microscopy-at-microscopy.com}
21, 25 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field
21, 25 -- Date: Thu, 5 Feb 2009 10:36:14 -0500
21, 25 -- Message-ID: {9A8F23892CB340BA8B03AB7048159FEE-at-Ken}
21, 25 -- MIME-Version: 1.0
21, 25 -- Content-Type: text/plain;
21, 25 -- charset="iso-8859-1"
21, 25 -- X-Priority: 3 (Normal)
21, 25 -- X-MSMail-Priority: Normal
21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 25 -- Importance: Normal
21, 25 -- Thread-Index: AcmHq8m0BcyacC7XQ2iypTQc+cCrEAABL/Eg
21, 25 -- In-Reply-To: {200902051606.n15G6mV9017413-at-ns.microscopy.com}
21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 25 -- Content-Transfer-Encoding: 8bit
21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15GZPeJ011759
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Thu, 5 Feb 2009 10:37:41 -0600
Subject: [Microscopy] Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ron and Listers,

In regards to the thread on Bio SEM and also the past thread from Frank Karl on trying to “see†Beryllium with EDS I would like to ask the community if the concept of the EDS optic to improve light element analysis would benefit applications in Bio SEM?

Dr Peter Ingram mentioned past use of EDS to find Be in human lung tissues, I believe that an application such as this would have benefited from the ability of acquiring up to 10 times the Be signal by the addition of this device.

I also believe that other applications exist and the optic qualifies as a new tool to increase the capabilities in many applications across the board including many in Bio SEM analysis.

We are in the midst of preparing a paper for the M&M conference and are looking for applications and samples that may benefit from this product. If you have any applications, samples, questions, or would like to talk about this please contact me offline.

--
Joe Ullmer

JoeXray LLC
7958 Dubois Road
Carlisle, OHIO 45005
OFFICE / FAX: 937 550-9224
Cell: 937 520-3811
joexray-at-cinci.rr.com
www.joexray.net



---- lherault-at-bu.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Our lab deals with Dental biomaterials and we have an SEM. The biological
} part comes into play for tissue engineering, e.g. seeing if cells are in
} scaffolds, are they happy with the surface they are on, etc. We've also
} looked at bacteria in microleakage studies. Some of these problems are
} unsolved but improved upon in 20 years. From what I've seen of research,
} the more diverse the background you have, the more apt you are to come up
} with a better solution to a problem. Remember Edison, the inventor of the
} phonograph and practical light bulb? He had to come up with a complete
} system to make the light bulb work outside of the lab. He studied how the
} gas company distributed gas, an already tried and true system. And the
} woman who invented the circular saw blade did so because she was using a
} spinning wheel while the males of her family were pushing and pulling a saw
} through wood. It's connections of old and new.
}
} Ron L
}
} -----Original Message-----
} X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
} Sent: Thursday, February 05, 2009 12:13 AM
} To: lherault-at-bu.edu
} Subject: [Microscopy] Current biological SEM
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} So, I thought I sent the following message to the list, but it never
} showed up. I looked at again and wondered if I should resend it, at
} first I wasn't going to do it, but then I decided, why not. After
} reviewing it I thought it might be a little naive, but I am curious
} about what you think.
}
} Here it is:
}
} Greetings
}
} At the risk of being labeled a heretic, I'd like to know the current
} state of biological SEM.
}
} Here's the deal, I am teaching a class on bio SEM and just sat down to
} prepare a lecture on modern biological SEM. I have lots of nice books
} with cool SEM pictures, but I noticed they are all about 20 years old.
} Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
}
} I tried to cruise the web to see if I could get up to date on what's
} happening, but web access to journals from here is pretty lame. So is
} the offering of ultrastructural type journals at our library, like,
} there aren't any.
}
} I do get a few journals and I can access a few on the web, but most of
} the papers in the searchable index from JCB etc. that have SEM are,
} well, pretty old. Is there much modern research using SEM being done
} in biology these days? Mostly I see the SEM being applied to materials
} type research. Not that the biology work that has been done is not
} elegant, it just seems like a lot of it is finding its way on to
} journal covers or 'coffee table' picture books after being colorized
} in Photoshop.
}
} I am OK with that, and if the kind of SEM that I know, simple fix,
} dehydr, and CPD is the state of the art, then I will feel better
} getting my students to do these things. But if there are sources for
} something new, I would like to be able to tell them about it. I mean,
} after all,how many fly eyes, bee's knees, and pollen grains can you
} look at?
}
} Out on a limb, and with my asbestos suit fully zipped,
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original Headers==============================
} 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009
} 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu
} [207.62.178.150])
} 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n155A6nj005730
} 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07
} -0600
}
}
} ==============================Original Headers==============================
} 22, 22 -- From lherault-at-bu.edu Thu Feb 5 08:42:34 2009
} 22, 22 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62])
} 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EgX5d032184
} 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:42:34 -0600
} 22, 22 -- X-Envelope-From: lherault-at-bu.edu
} 22, 22 -- Received: from BUSDM801203 ([155.41.214.83])
} 22, 22 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n15EgOoE002292
} 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:42:24 -0500
} 22, 22 -- From: "Ron L" {lherault-at-bu.edu}
} 22, 22 -- To: {microscopy-at-microscopy.com}
} 22, 22 -- References: {200902050512.n155Cjea010347-at-ns.microscopy.com}
} 22, 22 -- Subject: RE: [Microscopy] Current biological SEM
} 22, 22 -- Date: Thu, 5 Feb 2009 09:42:22 -0500
} 22, 22 -- Message-ID: {002401c9879f$f49d5030$53d6299b-at-ad.bu.edu}
} 22, 22 -- MIME-Version: 1.0
} 22, 22 -- Content-Type: text/plain;
} 22, 22 -- charset="us-ascii"
} 22, 22 -- Content-Transfer-Encoding: 7bit
} 22, 22 -- X-Mailer: Microsoft Office Outlook 11
} 22, 22 -- In-Reply-To: {200902050512.n155Cjea010347-at-ns.microscopy.com}
} 22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
} 22, 22 -- Thread-index: AcmHUGgTbUE/6JKoSnSCkEhcCgwrDgATlNQg
} ==============================End of - Headers==============================


==============================Original Headers==============================
11, 21 -- From joexray-at-cinci.rr.com Thu Feb 5 10:37:41 2009
11, 21 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Gbdx6013092
11, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:37:40 -0600
11, 21 -- Received: from hrndva-web17-z02 ([10.128.132.168])
11, 21 -- by hrndva-smta01.mail.rr.com with ESMTP
11, 21 -- id {20090205163836.RXHD4991.hrndva-smta01.mail.rr.com-at-hrndva-web17-z02} ;
11, 21 -- Thu, 5 Feb 2009 16:38:36 +0000
11, 21 -- Message-ID: {20090205163836.DOBTT.16831.root-at-hrndva-web17-z02}
11, 21 -- Date: Thu, 5 Feb 2009 11:38:36 -0500
11, 21 -- From: {joexray-at-cinci.rr.com}
11, 21 -- To: lherault-at-bu.edu
11, 21 -- Subject: Re: [Microscopy] RE: Current biological SEM
11, 21 -- Cc: Microscopy-at-microscopy.com
11, 21 -- In-Reply-To: {200902051458.n15Ewr8S022318-at-ns.microscopy.com}
11, 21 -- MIME-Version: 1.0
11, 21 -- Content-Type: text/plain; charset=utf-8
11, 21 -- X-Priority: 3 (Normal)
11, 21 -- Sensitivity: Normal
11, 21 -- Content-Transfer-Encoding: 8bit
11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15Gbdx6013092
==============================End of - Headers==============================




From: bob.price-at-uscmed.sc.edu
Date: Thu, 5 Feb 2009 10:45:40 -0600
Subject: [Microscopy] viaWWW: Basic Confocal Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bob.price-at-uscmed.sc.edu as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bob.price-at-uscmed.sc.edu
Name: Bob Price

Organization: University of South Carolina School of Medicine

Title-Subject: [Filtered] Basic Confocal Microscopy Workshop

Question: The University of South Carolina School
of Medicine will again be hosting a Basic
Confocal Microscopy Workshop. This yearís
workshop will be from June 15-19, 2009 and will
include a series of lectures on the theory and
applications of confocal microscopy, specimen
preparation, processing confocal images in
Photoshop, and 3D reconstructions using AMIRA.
Students will be able to process triple labeled
samples (cell cultures and sections) on site or
bring their own samples to the workshop.

Faculty will include Drs. Jay Jerome
(Vanderbilt), Ralph Albrecht (Univ
Wisconsin-Madison), John Mackenzie (North
Carolina State Univ), Tom TrusK (Medical Univ
South Carolina) and myself. Instruments and
applications experts from Leica, Nikon, Olympus,
Perkin Elmer, Photometrics, and Zeiss are
expected to be available for hands on training
and imaging of samples.

Please register early as the last 4 workshops
have been overbooked. Deadline for registration
is June 1 as long as slots are available.

For further information and registration go to:
http://dba.med.sc.edu/irf/price/irf/irf.htm or
contact Anna McNeal (Anna.McNeal-at-uscmed.sc.edu)

Bob

Bob Price
Research Professor
Dept Cell Biol and Anat
USC School of Medicine
6439 Garner's Ferry Road
Columbia, SC 29208

Tel: 803-733-3392
Admin Tel: 803-253-5822
Fax: 803-733-3212


Login Host: 129.252.69.40
---------------------------------------------------------------------------


==============================Original Headers==============================
14, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:45:40 2009
14, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15GjaUY026662
14, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:45:39 -0600
14, 13 -- Mime-Version: 1.0
14, 13 -- Message-Id: {p06240800c5b0c8651f8c-at-[206.69.208.22]}
14, 13 -- Date: Thu, 5 Feb 2009 10:46:49 -0600
14, 13 -- To: microscopy-at-microscopy.com
14, 13 -- From: bob.price-at-uscmed.sc.edu (by way of MicroscopyListserver)
14, 13 -- Subject: viaWWW: Basic Confocal Microscopy Workshop
14, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
14, 13 -- Content-Transfer-Encoding: 8bit
14, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15GjaUY026662
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Thu, 5 Feb 2009 11:25:27 -0600
Subject: [Microscopy] Puzzling EDX results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Note: EDX map not attached to Listserver copy)
Dear Debbie,
There are a number of ways that this analysis may be compromised. I have
attached a low magnification EDX map of the surface of standard solder. The
lead is green and the tin is red. You can see how the phases segregate and
the composition would vary greatly, depending on where and how large an area
you analysed. The size of the various phases and grains will depend on the
cooling rate of the solder.
The other consideration is the state of the surface. EDX can only give
reasonable analysis if the sample surface is flat and normal to the beam, so
the x-ray take-off angle and solid angle are well-known and accurately
entered into the EDX software. I always say that if you lie to the computer,
he will give you the wrong answer. Everyone wants to get the numbers out,
but they can be wildly inaccurate or imprecise if the geometry is wrong or
unknown. If you are looking at a round wire, make sure that you are looking
at the top, where it approximates a flat, normal-to-the-beam surface. In
your case, I would take five to ten readings in different spots along the
wire and average them, to get the overall solder content.
I have the same problem when I look at leaded brass, where the lead is not
alloyed with the brass, but remains in discreet little balls to act as a
lubricant in machining. The analysis shows either no lead or 10% lead, when
you hit a ball.
Good luck. Explaining the pitfalls of the magic EDX machine is the hardest
part of the job.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: February 4, 2009 7:15 PM
To: maryflet-at-interchange.ubc.ca

Hi all,

We were analyzing some samples of tin solder with varying but small amounts
of lead. The solder was electroplated as a film (~7-10µm) on copper. We
need to know % of lead in the samples with reasonable accuracy. The amounts
of Pb varied from about 0.5 to 3% based on information from another
quantifying technique.

We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and
then repeated this on the same area two other times under identical
conditions and immediately following the previous spectrum. Each time the
ratio of Sn to Pb varied significantly. We are at a loss to explain why
there was such a difference in the ratios from the identical area. Could
this be due to something going on due to the repeated sampling of the same
area?

Any idea as to why we had such poor reproducibility?

Thanks,
Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy






==============================Original Headers==============================
12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009
12, 30 -- Received: from mailhub128.itcs.purdue.edu
(mailhub128.itcs.purdue.edu [128.210.5.128])
12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1537wD4026640
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58
-0600
12, 30 -- Received: from mailhub126.itcs.purdue.edu
(mailhub126.itcs.purdue.edu [128.210.5.126])
12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n1537wpL022381
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58
-0500
12, 30 -- Received: from 1061exfe01a.itap.purdue.edu
(1061exfe01a.itap.purdue.edu [128.210.1.8])
12, 30 -- by mailhub126.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609
12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58
-0500
12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by
1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500
12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl
([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu
([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ;
12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000
12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119
12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500
12, 30 -- Subject: Puzzling EDX results
12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu}
12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu}
12, 30 -- Thread-Topic: Puzzling EDX results
12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA==
12, 30 -- Mime-version: 1.0
12, 30 -- Content-type: text/plain;
12, 30 -- charset="ISO-8859-1"
12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC)
FILETIME=[F2BAF2B0:01C9873E]
12, 30 -- X-PMX-Version: 5.4.0.320885
12, 30 -- X-PerlMx-Virus-Scanned: Yes
12, 30 -- Content-Transfer-Encoding: 8bit
12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1537wD4026640
==============================End of - Headers==============================



==============================Original Headers==============================
23, 31 -- From maryflet-at-interchange.ubc.ca Thu Feb 5 11:25:25 2009
23, 31 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5])
23, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15HPNNF008069
23, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:25:24 -0600
23, 31 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
23, 31 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id 7332B1820E
23, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:27:32 -0800 (PST)
23, 31 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
23, 31 -- by smtp.interchange.ubc.ca
23, 31 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
23, 31 -- with ESMTP id {0KEL00FYDSHV75-at-smtp.interchange.ubc.ca} for
23, 31 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:27:32 -0800 (PST)
23, 31 -- Date: Thu, 05 Feb 2009 09:27:21 -0800
23, 31 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
23, 31 -- Subject: FW: [Microscopy] Puzzling EDX results
23, 31 -- To: microscopy-at-microscopy.com
23, 31 -- Reply-to: maryflet-at-interchange.ubc.ca
23, 31 -- Message-id: {0KEL00FYESHV75-at-smtp.interchange.ubc.ca}
23, 31 -- Organization: Materials Eng.
23, 31 -- MIME-version: 1.0
23, 31 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 31 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
23, 31 -- Content-type: text/plain; charset=iso-8859-1
23, 31 -- Thread-index: AcmHP+h99ywAcSieSmaj/M7083feXwAdA7tAAAC6EoA=
23, 31 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.2.5.171326
23, 31 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
23, 31 -- X-PerlMx-Spam: Probability=8%, Report=__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0
23, 31 -- X-Spam-Level:
23, 31 -- X-Spam-Flag: No
23, 31 -- Content-Transfer-Encoding: 8bit
23, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15HPNNF008069
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Thu, 5 Feb 2009 10:47:13 -0600
Subject: [Microscopy] viaWWW: Parallax Research is looking for reps to hire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello (again) All:

I need to clarify my request yesterday regarding hiring reps and
distributors, based partly on the response I received.

Parallax is looking to hire INDEPENDENT manufacturer's reps and distributors
for our new x-ray microanalysis products (LoMAX EDS optic, WDS, EDS, x-ray
components). If you know such a person, or company, in your area (or
others) that you are familiar with and like, I would greatly appreciate a
recommendation. Please give them my contact info, or provide me their
contact info. We are interested in talking to anyone who is interested.

Thank you again for your time and attention.

Regards,

Don


Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.

Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com


-----Original Message-----
X-from: dkloos-at-parallaxray.com [mailto:dkloos-at-parallaxray.com]
Sent: Wednesday, February 04, 2009 12:32 PM
To: dkloos-at-parallaxray.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dkloos-at-parallaxray.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: dkloos-at-parallaxray.com
Name: Don Kloos

Organization: Parallax Research, Inc.

Title-Subject: [Filtered] Parallax Research is looking for reps to hire

Question: Hello All:

I need your help. Parallax Research is seeking to hire
manufacturer's reps and distributors to handle its new EDS and WDS
x-ray microanalysis products in North America, Europe, Asia, and
other regions of the world. If you know of an individual or company
in your area that you like to deal with and can recommend them,
please let me know.

Thank you for your time.

Don Kloos
Parallax Research.
Email: dkloos-at-parallaxray.com.
Phone: 1 866 581-XRAY, or +1 714 897-9779.
www.parallaxray.com



Login Host: 24.136.64.169
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Feb 4 14:24:02 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n14KO2Ja000532
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 14:24:02
-0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c5afa9d3f3e1-at-[206.69.208.22]}
11, 11 -- Date: Wed, 4 Feb 2009 14:24:01 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dkloos-at-parallaxray.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Parallax Research is looking for reps to hire
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
24, 30 -- From dkloos-at-parallaxray.com Thu Feb 5 10:47:11 2009
24, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Gl8U7028798
24, 30 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:47:10 -0600
24, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
24, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
24, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
24, 30 -- id 1LV7Or-0007P2-KO; Thu, 05 Feb 2009 09:48:25 -0700
24, 30 -- Reply-To: {dkloos-at-parallaxray.com}
24, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
24, 30 -- To: {dkloos-at-parallaxray.com}
24, 30 -- Cc: {microscopy-at-microscopy.com}
24, 30 -- References: {200902042032.n14KW4WP013753-at-ns.microscopy.com}
24, 30 -- Subject: RE: [Microscopy] viaWWW: Parallax Research is looking for reps to hire
24, 30 -- Date: Thu, 5 Feb 2009 08:48:17 -0800
24, 30 -- Organization: Parallax Research
24, 30 -- Message-ID: {ADEEA35FEA604A2DAF4123C8C7845A63-at-donl}
24, 30 -- MIME-Version: 1.0
24, 30 -- Content-Type: text/plain;
24, 30 -- charset="US-ASCII"
24, 30 -- Content-Transfer-Encoding: 7bit
24, 30 -- X-Mailer: Microsoft Office Outlook 11
24, 30 -- In-reply-to: {200902042032.n14KW4WP013753-at-ns.microscopy.com}
24, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 30 -- Thread-Index: AcmHB6kzNd+EzppESJy4fs1acClo5gAqN67Q
24, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
24, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
24, 30 -- X-AntiAbuse: Original Domain - microscopy.com
24, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
24, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Thu, 5 Feb 2009 11:42:51 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hans,

Situation with EMI strongly depends on what kind of field are you dealing
with, what is the frequency of magnetic interference. DC field,
low-frequency (like 50Hz from power line) and high frequency (RF and HF
portion of spurious fields from sparks) all behave differently. To get
better idea about the nature of the problem you need to do EMI survey and
obtain a plot of field vs. frequency. If this was already done then posting
a link to the plot of the results could be helpful.

Without going too deep into the details, restoring faraday cage will help
with RF, but would do nothing for 50Hz or DC field. Because of substantial
thickness of the steel walls (2cm) restoring continuity of magnetic cage
will measurably weaken your 50Hz and higher frequency magnetic noise. By the
way - is the floor also shielded by the same 2cm steel? If your field is DC
then it may actually be coming from magnetized steel cage itself (Curious:
is it really steel and not a magnetically soft iron?) In this case you can
try to de-magnetize it.

About four years ago I had great experience with magnetic field cancellation
system from http://www.spicerconsulting.com/ - talk to them about your
particular case and try to see if they can arrange a demo setup at your site
to see how much improvement would you get in your specific case. No interest
here, just a (former) but very satisfied customer.

Keep in mind that magnetic cancellation systems have certain response time.
They great in canceling steady-state or slow-changing AC fields, but it
takes some time to adjust cancellation in response to rapid change of the
interference. Therefore when and if you get occasional strong spark from the
tram or arc, welder some of it will get through the cancellation and may
potentially ruin that particular image you were taking.

Hope this helps, if you have more questions please feel free to contact on
or off-list.

Cheers,
Valery Ray

============================
www.partbeamsystech.com
PBS&T, MEO Engineering Co, Inc.
290 Broadway St., Suite 298
Methuen, MA 01844
Phone: (978) 296-5063

-----Original Message-----
X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl]
Sent: Thursday, February 05, 2009 11:03 AM
To: vray-at-partbeamsystech.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.janssen-at-nki.nl
Name: Hans Janssen

Organization: Netherlands Cancer Institute

Title-Subject: [Filtered] How to get rid of a magnetic field

Question: We want to move our EMís to a building
close to tram tracks. At pre-installation
measurements by FEI a distorting field of 10
mGauss (4.4 mGauss is the limit for a Tecnai12)
was found. This building was a former MRI
location; in the walls are steel (2cm thick)
plating and a copper Faraday cage both with large
holes in the ceiling. Can you give me advice on
preventing this magnetic field? Is restoring the
Faraday cage and/or the steel cage enough? Or do
we need a mu-ferro or a Helmholtz cage? What will
be the approximate costs of these?

Best regards, Hans


Login Host: 194.171.7.39
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009
9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n15G17MS011832
9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09
-0600
9, 13 -- Mime-Version: 1.0
9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]}
9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600
9, 13 -- To: microscopy-at-microscopy.com
9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver)
9, 13 -- Subject: viaWWW: How to get rid of a magnetic field
9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 13 -- Content-Transfer-Encoding: 8bit
9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n15G17MS011832
==============================End of - Headers==============================



==============================Original Headers==============================
24, 26 -- From vray-at-partbeamsystech.com Thu Feb 5 11:42:50 2009
24, 26 -- Received: from smtp100.biz.mail.re2.yahoo.com (smtp100.biz.mail.re2.yahoo.com [206.190.52.46])
24, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n15Hgmjd024807
24, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:42:49 -0600
24, 26 -- Message-Id: {200902051742.n15Hgmjd024807-at-ns.microscopy.com}
24, 26 -- Received: (qmail 74115 invoked from network); 5 Feb 2009 17:45:29 -0000
24, 26 -- Received: from unknown (HELO cp1198275a) (vray-at-75.67.14.43 with login)
24, 26 -- by smtp100.biz.mail.re2.yahoo.com with SMTP; 5 Feb 2009 17:45:29 -0000
24, 26 -- X-YMail-OSG: 322IYPAVM1nxyVFrJLWbhPO4NGejQsrDZyW24Wx_SNz0fFzkvshuxXy6chafZLvbiACdkNH9fJhdnE0wcPH0z4Jmh6hEc2e5b9cWuPGLFW7nZ4I_GImp3hM3qZefrkTWCSu6LHVYf_J43dLdrDUixVz9x8IhZjQwhOEbI56KoH2ZChoF9TjKGPSVUyOvBmR_GB2v3A4nqZ8oQykTpp3BnXXicb7TnCZE
24, 26 -- X-Yahoo-Newman-Property: ymail-3
24, 26 -- Reply-To: {vray-at-partbeamsystech.com}
24, 26 -- From: "Valery Ray" {vray-at-partbeamsystech.com}
24, 26 -- To: {j.janssen-at-nki.nl}
24, 26 -- Cc: {microscopy-at-microscopy.com}
24, 26 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field
24, 26 -- Date: Thu, 5 Feb 2009 12:48:22 -0500
24, 26 -- Organization: PBST / MEO Engineering
24, 26 -- MIME-Version: 1.0
24, 26 -- Content-Type: text/plain;
24, 26 -- charset="iso-8859-1"
24, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
24, 26 -- Thread-Index: AcmHqz47KwN3pd6URkidOnTRaHrKGAACSVQg
24, 26 -- In-Reply-To: {200902051602.n15G2vR4013419-at-ns.microscopy.com}
24, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 26 -- Content-Transfer-Encoding: 8bit
24, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15Hgmjd024807
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Thu, 5 Feb 2009 12:38:30 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Regarding the 'biosem' and 'envirosem' topic, can somebody explain how light
element analysis is (or isn't) done with EDS and WDS in these circumstances?

Can light elements be analyzed in these environments and is this even done?

Thanks,

Don

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.



Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu]
Sent: Thursday, February 05, 2009 12:15 AM
To: dkloos-at-parallaxray.com

Jonathan,
You ask a relevant question. As a biologist who has gotten
deeply into SEM in the last decade, I am surprised that there is not
more use on our side of the fence. I suspect that most of the recent
advances in SEM tech have been driven my materials folks and that the
biologists simply don't know about them. Let me give you my
perspective on where I think the advances are.

1. Environmental SEM. It is now possible to examine a fully hydrated
specimen in the SEM. The sample needs to be frozen or at least cold
to minimize evaporation but otherwise not processed at all. I am
aware of people who use ESEM to study root hairs and also floral
meristems. I suspect animal scientists use also. The point is that
fixation crtical point dry and coating are all avoided and instead
the sample is directly viewed. The drawback is that once the sample
is out of the machine it cannot be viewed again later. And the
resolution of an ESEM is no better than a conventional tungsten
filament model.

2. Wet chambers. A rather recent (5 years or so?) development are
thin membranes that the beam can pass but gas cannot. This allows
fully hydrated samples to be viewed at ambient temp and pressure in
chambers built with the top surface being that special membrane. Thus
living cells can be grown on the membrane (inside the membrane) and
viewed in the SEM while they are alive. It is of course a question
how much radiation damage the beam will induce. Still the cells are
certanly viable for a while. I have seen papers using this tech on
animal tissue culture cells. There was quite a lot of excitement when
these chambers hit the market but I don't know how successful they
have proven in the "real" world.

The above points are geared for eliminating fixation and looking at
samples as close to living as possible. This is clearly difficult
given the fundamental high-vacuum nature of the SEM beast. The other
direction where advances lay is in the high vac, high resolution end
of things. Here the advances in SEM design have been phenomenal. The
field emission gun allows resolution to be obtained that is almost as
good as the best TEMs and certainly far beyond what the good old fly
eye SEM was capable of seeing.

3. High resolution. With the field emission gun, macromolecules can
be resolved. I among others have taken advantage of this to study the
ultrastructure of the plant cell wall. Cellulose microfibrils are on
order of 10 nm and these can be readily imaged with FESEM. What is
particularly useful for me is that I can put my plant stem 1 mm by 10
mm in to instrument, get good low mag survey views and crank up the
magnficiation to the ultrastructual level in any cell on the sample
that is appropriate. The easy moving between low and high
magnifications is extremely helpful where extensive sampling is
needed or when rare features need to be found. Other biological
systems that have been profitably studied with FESEM include the
cytoskeleton, bacterial biofilms, biomineralization, and nuclear
pore complexes. All of these cases involve complex three dimensional
structures and the FESEM lets you image them at high resolution in
the intact (or semi-intact) state.

4. Low voltage. Along with the sharper probe of the FESEM comes the
ability to work at really low accelerating voltage. In the recent
vintage FESEM's it is easy to image at 1 kV and possible to go down
even as low as a tenth of that. This has several advantages. First,
many samples are destroyed or damaged at 30 kV or even at 10 kV.
Second, the lower the beam voltage the more exclusively the image
comes from the surface. This increases the resolution in the depth
direction.

Both low voltage and the smaller probe size allow much thinner metal
coats to be applied, and in some cases no coat at all. For the work I
have been doing on the cell wall, I use ca. 1 to 2 nm of Pt. This is
an order of magnitude less than typical gold coats for conventional
tungsten SEM work.

5. Backscattered imaging. Of particular importance to biologists is
being able to image backscattered electrons. This allows the
localization of gold probes, as in gold conjugated secondary
antibodies. With the latest instrumentation, one can colect electrons
from selected energy (or angle) levels and mix signals from several
detectors. Thus, the SE detector can show the topography of the
sample while the BSE detector can pick up the signal from the gold
and the two are overlain seamlessly. The sensitivity of the detectors
is such that the gold can be imaged at high contrast even with a
sample lightly covered with platinum. I took advantage of this (with
slightly older not quite so performant technology) to detect gamma
tubulin in the plant cell cortex. Others have localized antigens on
the plasma membrane and cell wall, to name a few.

There have been parallel developments in elemental analysis (EDS and
related modes) and I expect biologists have taken advantage of these
too but it is not my area so I don't know. Perhaps a helpful netizen
will chime in.

Summarizing, you are right, recent use of SEM in biology is a little
bit burried. However these uses will be well worth your digging up.
Good luck with your course,
Tobias

}
}
} Greetings
}
} At the risk of being labeled a heretic, I'd like to know the current
} state of biological SEM.
}
} Here's the deal, I am teaching a class on bio SEM and just sat down to
} prepare a lecture on modern biological SEM. I have lots of nice books
} with cool SEM pictures, but I noticed they are all about 20 years old.
} Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
}
} I tried to cruise the web to see if I could get up to date on what's
} happening, but web access to journals from here is pretty lame. So is
} the offering of ultrastructural type journals at our library, like,
} there aren't any.
}
} I do get a few journals and I can access a few on the web, but most of
} the papers in the searchable index from JCB etc. that have SEM are,
} well, pretty old. Is there much modern research using SEM being done
} in biology these days? Mostly I see the SEM being applied to materials
} type research. Not that the biology work that has been done is not
} elegant, it just seems like a lot of it is finding its way on to
} journal covers or 'coffee table' picture books after being colorized
} in Photoshop.
}
} I am OK with that, and if the kind of SEM that I know, simple fix,
} dehydr, and CPD is the state of the art, then I will feel better
} getting my students to do these things. But if there are sources for
} something new, I would like to be able to tell them about it. I mean,
} after all,how many fly eyes, bee's knees, and pollen grains can you
} look at?
}
} Out on a limb, and with my asbestos suit fully zipped,
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of
Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
12, 21 -- From baskin-at-bio.umass.edu Thu Feb 5 02:05:58 2009
12, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu
[128.119.55.19])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1585wa3024378
12, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 02:05:58
-0600
12, 21 -- Received: from [133.48.48.239] (dh48-239.nibb.ac.jp
[133.48.48.239])
12, 21 -- (authenticated bits=0)
12, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id
n1585pIE020660
12, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NO);
12, 21 -- Thu, 5 Feb 2009 03:05:54 -0500 (EST)
12, 21 -- Mime-Version: 1.0
12, 21 -- Message-Id: {p06240538c5b0451e1d83-at-[133.48.48.239]}
12, 21 -- In-Reply-To: {200902050510.n155AuFl006549-at-ns.microscopy.com}
12, 21 -- References: {200902050510.n155AuFl006549-at-ns.microscopy.com}
12, 21 -- Date: Thu, 5 Feb 2009 17:05:50 +0900
12, 21 -- To: jkrupp-at-deltacollege.edu
12, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
12, 21 -- Subject: Re: [Microscopy] Current biological SEM
12, 21 -- Cc: microscopy-at-microscopy.com
12, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
12, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by
milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Thu, 05 Feb 2009
03:05:56 -0500 (EST)
12, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================


==============================Original Headers==============================
24, 30 -- From dkloos-at-parallaxray.com Thu Feb 5 12:38:30 2009
24, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15IcRBg016553
24, 30 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 12:38:29 -0600
24, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
24, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
24, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
24, 30 -- id 1LV97V-000H2t-7T; Thu, 05 Feb 2009 11:38:37 -0700
24, 30 -- Reply-To: {dkloos-at-parallaxray.com}
24, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
24, 30 -- To: {baskin-at-bio.umass.edu}
24, 30 -- Cc: {microscopy-at-microscopy.com}
24, 30 -- References: {200902050815.n158FAEq005140-at-ns.microscopy.com}
24, 30 -- Subject: RE: [Microscopy] Re: Current biological SEM
24, 30 -- Date: Thu, 5 Feb 2009 10:38:28 -0800
24, 30 -- Organization: Parallax Research
24, 30 -- Message-ID: {05E0E59A3FE44A72BFCE9304DD82DEA8-at-donl}
24, 30 -- MIME-Version: 1.0
24, 30 -- Content-Type: text/plain;
24, 30 -- charset="US-ASCII"
24, 30 -- Content-Transfer-Encoding: 7bit
24, 30 -- X-Mailer: Microsoft Office Outlook 11
24, 30 -- In-reply-to: {200902050815.n158FAEq005140-at-ns.microscopy.com}
24, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 30 -- Thread-Index: AcmHad3XYhxaS9V2SGaRaUEq+6j6jwAVrkcA
24, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
24, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
24, 30 -- X-AntiAbuse: Original Domain - microscopy.com
24, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
24, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: vlynch-at-mail.wsu.edu
Date: Thu, 5 Feb 2009 15:11:03 -0600
Subject: [Microscopy] RE: Philips TEM CM-200 Manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Jerzy,
Thank you for your response. We are in contact with our local FEI
service group they are the ones who told us that Philips recently
destroyed all it's manual copies for the Philips CM200. The service
engineer we are working with says he does not have acess to or cannot
find any form of publication about our scope. We are trying to write
operating protocols for general use, x-ray diffraction and EDAX and
we are trying to learn how to operate it so we can teach/help our
students, faculty and clients. If you have any step by step
instruction for x-ray diffraction and EDAX that would be very helpful.
Valerie

} Valerie,
}
} There are multiple large-volumes (300page and up) manuals for the
} CM-series instruments: Mechanical, Electronics, and couple of shorter
} publications from Philips electron Optics on operations. I suggest you
} contact your local FEI service group; they have CDs with those manuals
} in PDF format issued to service engineers, perhaps you can get a copy of
} them. I have Cm300FEG and many of the features between these instruments
} are similar, if you need specific stuff I might be able to scan few
} pages and e-mail them to you.
}
} I think the FEI call number for service is 1-888-ION-MILL.
}
} Jerzy
} ***************************************************
} Jerzy Gazda Ph.D.
} Section Manager - TEM, FIB, SEM
} Cerium Laboratories LLC
} 5204 E. Ben White Blvd. MS-512
} Austin, TX 78741
}
} (512) 934-5185 vm
} (512) 622-6600 pgr
}
} www.ceriumlabs.com
}
} -----Original Message-----
} From: vlynch-at-mail.wsu.edu [mailto:vlynch-at-mail.wsu.edu]
} Sent: Thursday, February 05, 2009 10:19 AM
} To: Gazda, Jerzy
} Subject: [Microscopy] Philips TEM CM-200 Manual?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America


==============================Original Headers==============================
3, 24 -- From vlynch-at-mail.wsu.edu Thu Feb 5 15:11:03 2009
3, 24 -- Received: from mx.wsu.edu.pph5a.pphosted.com (mx.wsu.edu.pph5a.pphosted.com [208.86.201.34])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15LB0ih006200
3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 15:11:02 -0600
3, 24 -- Received: from jaguar.it.wsu.edu (jaguar.it.wsu.edu [134.121.0.73])
3, 24 -- by mx.wsu.edu.pph5a.pphosted.com (8.14.1/8.14.1) with ESMTP id n15LAt26003077
3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 13:10:56 -0800
3, 24 -- Received: from [134.121.42.104] (vrf.emc.wsu.edu [134.121.42.104])
3, 24 -- by jaguar.it.wsu.edu (8.12.11.20060308/8.12.11) with ESMTP id n15LAtCY011566
3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 13:10:55 -0800
3, 24 -- Mime-Version: 1.0
3, 24 -- Message-Id: {a06200708c5b104172f0d-at-[134.121.42.104]}
3, 24 -- In-Reply-To:
3, 24 -- {B8013C1F41F45F4886A4F71F775DF1587383BF-at-USAUSEXMBPF1.spansion.com}
3, 24 -- References: {200902051618.n15GIswj003085-at-ns.microscopy.com}
3, 24 -- {B8013C1F41F45F4886A4F71F775DF1587383BF-at-USAUSEXMBPF1.spansion.com}
3, 24 -- Date: Thu, 5 Feb 2009 13:10:53 -0800
3, 24 -- To: Microscopy-at-microscopy.com
3, 24 -- From: Valerie Lynch-Holm {vlynch-at-mail.wsu.edu}
3, 24 -- Subject: RE: [Microscopy] Philips TEM CM-200 Manual?
3, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 24 -- X-Virus-Scanned: by amavisd-milter (http://amavis.org/)
3, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-05_03:2009-02-05,2009-02-05,2009-02-05 signatures=0
3, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0902050126
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Thu, 5 Feb 2009 15:52:55 -0600
Subject: [Microscopy] Scanner for Plate film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I like to scan plate film (Philips EM300) , can you please suggest whic
one is suitable for scaaning the EM films.

Thank you very much for your help.

V.Kabilan
UMDNJ-NJDS
Newark, USA


==============================Original Headers==============================
4, 26 -- From velliyka-at-umdnj.edu Thu Feb 5 15:52:55 2009
4, 26 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n15Lqs22022389
4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 15:52:54 -0600
4, 26 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
4, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 599E6A7B62
4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:52:52 -0500 (EST)
4, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
4, 26 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 6888BA7B28
4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:52:31 -0500 (EST)
4, 26 -- Received: from ([10.32.15.171])
4, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6189864;
4, 26 -- Thu, 05 Feb 2009 16:52:27 -0500
4, 26 -- MIME-version: 1.0
4, 26 -- Content-transfer-encoding: 7BIT
4, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
4, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
4, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
4, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KEM00EB04RFY9A0-at-umduwc02.umdnj.edu} for
4, 26 -- Microscopy-at-microscopy.com; Thu, 05 Feb 2009 16:52:27 -0500 (EST)
4, 26 -- Message-id: {7fd8e9f8590e21c7591a62ceac910a1c-at-umdnj.edu}
4, 26 -- To: Microscopy-at-microscopy.com
4, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
4, 26 -- Subject: Scanner for Plate film
4, 26 -- Date: Thu, 05 Feb 2009 04:55:32 -0500
4, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Thu, 5 Feb 2009 16:09:50 -0600
Subject: [Microscopy] RE: Scanner for Plate film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I prefer the Epson Perfection V750-M Pro Scanner. It costs around $700,
but the extra money is worth it for the extra software and calibration
standard that is included. It makes a pretty decent stand-in for a
stereoscopic microscope, too.

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.

-----Original Message-----


} I like to scan plate film (Philips EM300) , can you please suggest whic

} one is suitable for scaaning the EM films.
}
} Thank you very much for your help.
}
} V.Kabilan
} UMDNJ-NJDS
} Newark, USA



This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
11, 34 -- From Robert.Zonis-at-Sanford.com Thu Feb 5 16:09:50 2009
11, 34 -- Received: from mail142.messagelabs.com (mail142.messagelabs.com [216.82.249.99])
11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15M9nuW004441
11, 34 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:09:50 -0600
11, 34 -- X-VirusChecked: Checked
11, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
11, 34 -- X-Msg-Ref: server-8.tower-142.messagelabs.com!1233871787!65085875!1
11, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
11, 34 -- X-Originating-IP: [198.176.16.25]
11, 34 -- Received: (qmail 12669 invoked from network); 5 Feb 2009 22:09:47 -0000
11, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
11, 34 -- by server-8.tower-142.messagelabs.com with RC4-SHA encrypted SMTP; 5 Feb 2009 22:09:47 -0000
11, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
11, 34 -- Thu, 5 Feb 2009 16:09:46 -0600
11, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 34 -- Content-class: urn:content-classes:message
11, 34 -- MIME-Version: 1.0
11, 34 -- Content-Type: text/plain;
11, 34 -- charset="us-ascii"
11, 34 -- Subject: RE: [Microscopy] Scanner for Plate film
11, 34 -- Date: Thu, 5 Feb 2009 16:07:49 -0600
11, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401950A46-at-naoaksasebe02.nr.ad.newellco.com}
11, 34 -- In-Reply-To: {200902052159.n15LxEks031584-at-ns.microscopy.com}
11, 34 -- X-MS-Has-Attach:
11, 34 -- X-MS-TNEF-Correlator:
11, 34 -- Thread-Topic: [Microscopy] Scanner for Plate film
11, 34 -- Thread-Index: AcmH3P7d8bcKCLdLQA+8rheHYN/2EQAAGMCA
11, 34 -- References: {200902052159.n15LxEks031584-at-ns.microscopy.com}
11, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
11, 34 -- To: {velliyka-at-umdnj.edu}
11, 34 -- Cc: {microscopy-at-microscopy.com}
11, 34 -- X-OriginalArrivalTime: 05 Feb 2009 22:09:46.0856 (UTC) FILETIME=[74C88E80:01C987DE]
11, 34 -- Content-Transfer-Encoding: 8bit
11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15M9nuW004441
==============================End of - Headers==============================




From: sptinling-at-ucdavis.edu
Date: Thu, 5 Feb 2009 16:14:10 -0600
Subject: [Microscopy] Image Processing -- Ethics and Validity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I am new here and this is my first response after
reading Netnotes in Microscopy Today for
years. Thanks for all the great
information. Please pardon any initial unintended errors in protocol.
I am an old dog learning new tricks so I would
like members of this discussion to consider
reconciling a couple of concepts. 1. Materials
and Methods – complete reporting. 2. Significant
figures (mathematically speaking).
I was trained for EM and photography back in the
days of glass plates and drum dryers with
Pacasol. I now work in an age where we have
discarded all our film photography materials and
work with digital cameras and software. In the
old days, dogging and burning were standard
techniques in the dark room to get your EM
picture to look perfect. No white spots from
dust were allowed, and bits of stain in the lumen
of an open perfused blood vessel were eliminated
with a conveniently placed label and arrow. That
was never considered as altering the significance
of the data which the image conveyed and that was
by an instructor who always stressed complete
descriptions in the materials and methods section
of everything that was done, all equipment used,
etc. No one ever reported that the left corner
of Fig.1 was dogged for 3 seconds while printing
on grade 3 paper with the developer at 22 degrees
centigrade. I have done enough EM to know that
sometimes the picture you end up with has
aesthetic defects. I am equally sure that most
in not all the published EM (or LM, SEM, etc)
images have that conveniently placed arrow or
label somewhere. Should that have been reported
then and or now? How is it any different if you
remove what you know to be dirt or a stain
particle from an image by any digital subtraction
or addition or the rubber stamps method then by
placing that convenient label or arrow over it
and then merging the layers? You still have
changed pixel values. I personally do not see
the difference nor do I think it necessary to
state in the figure legend or an appendix all the
aesthetic alterations to an image. Also, I can
not image a journal allowing you to. Even with
online publishing I am constantly being told by
the journals to make things shorter.

As for the data contained in the digital image,
the amount contained in individual pixel values
and pixels per image goes way beyond the number
of significant figures relevant to the data being
presented by the image. And before you even get
to Photoshop, the camera software lets you alter
the image intensity, number of bits in an image
(8, 12, 16) etc. To me it makes perfect sense to
have a dark and light background image (as
previously mentioned) and apply them to the image
in question to eliminate defects in the optical
path and report that in the M&M. However, if
the point of the picture in question depends on
the number of an individual pixel or the numbers
of pixels in an image, then it makes sense to
include any alterations in the M&M
section. However, I suspect that for a very
large percentage of the images published, a
rubber stamped alteration of a capillary lumen
for aesthetic purposes would be immaterial even
though it changed the original values of the
pixels in question. I would never consider that
scientific fraud. Even when the pixels in
question might have significance, when does the
issue of reporting significant figures come in to
the issue? I can accept that there are images
produced where the data from the image are the
pixel values. But, as I am mostly biologically
oriented that does not happen very often to
me. There are lots of papers out there in the
early days of immunohistochemistry which tried to
equate stain intensity (the number of a given
pixel) with amount of pathology (numbers of
pixels equal to or greater than a given pixel
value per image with the right RGB or CMY or HIS,
etc values) only to discover that the errors
derived from trying to mix those two parameters
yielded only false results. As my EM instructor
always pointed out, everything we report is an
“artifact”. So, if pixel values are that
important, when do we apply the test of
significant figures to the pixel data. If I
publish 1 picture from the skin of 1 test animal
showing “representative” damage to the basal
lamina in an experimental group (lets say 15
control and 15 experimental animals) then anyone
who accepts my picture might further question the
M&M section, for number of images taken per
section, per sample, per animal and further ask
if the data were derived from a sterologic
process of sampling or whether I just got
lucky. I rarely see any of this data
reported. I realize as was said earlier that the
day is coming when there will be enough space on
the journal’s server for them to require that the
original unaltered image be placed there along
with the published one (in which you placed that
convenient arrow) but then how do we know that an
unaltered image was placed there in the first
place? We do not -- we trust in the integrity
within our community, and although fraud does
occur is it at such a rate that it is really an
issue? I suppose the well trained individual
could download the original of a “questionable”
published image and subject it to an analysis
which would show pixel alterations and that would
be good if one suspected deliberate fraud. My
guess is that it would be a very rare thing. To
close, I am a firm believer in complete M&M
reporting and that would include how I took my
image – camera, microscope, software, and
techniques used, but I can not see burdening
readers with the morally appropriate mea culpa
that I typed a figure label or placed an arrow
over an aesthetic defect and merged the layers to
make a picture more aesthetically pleasing, or
that I “rubberstamped” a piece of dirt. Thanks
for considering these issues. Steve

Steven P. Tinling Ph.D.
Director of Research
Otolaryngology Research Lab
University of California, Davis
1515 Newton Ct., Rm. 209
Davis, CA. 95616-4859
Phone: 530-754-5045
Fax: 530-754-5046



==============================Original Headers==============================
5, 22 -- From sptinling-at-ucdavis.edu Thu Feb 5 16:14:10 2009
5, 22 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15ME9O8011215
5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb 2009 16:14:10 -0600
5, 22 -- Received: from MOTO-2951.ucdavis.edu ([128.120.223.66])
5, 22 -- (authenticated bits=0)
5, 22 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n15ME3sK026356
5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb 2009 14:14:04 -0800 (PST)
5, 22 -- Message-Id: {200902052214.n15ME3sK026356-at-warsaw.ucdavis.edu}
5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
5, 22 -- Date: Thu, 05 Feb 2009 14:14:28 -0800
5, 22 -- To: Microscopy-at-Microscopy.Com
5, 22 -- From: Steve Tinling {sptinling-at-ucdavis.edu}
5, 22 -- Subject: Image Processing -- Ethics and Validity
5, 22 -- Mime-Version: 1.0
5, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
5, 22 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av5
5, 22 -- X-Virus-Status: Clean
5, 22 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15ME9O8011215
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Thu, 5 Feb 2009 16:21:45 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The perspective from an institution focused almost entirely on human
medical and related biological research studies puts SEM in the far
background. Whereas, there was once several SEM instruments on campus
there is now only one old, mostly inaccesible instrument in the Dental
School. I might hear an inquiry about SEM resources once a year and I
have to direct those investigators to a lab at another institution that
enjoys a full spectrum of departments and science fields. The vast
majority of funding and interest primarily focuses on genetic and
molecular questions. The SEM does not provide much useful data for these
studies though it is invaluable for descriptive illustrations. I think
this is supported by the dearth of SEM data in mamalian biology studies
in high profile publications such as Science, Nature and Cell. In
contrast light microscopy, particularly laser scanning confocal
microscopy, is booming. I estimate that there are over 30 heavily used
confocal systems at this university and light micrographs are commonly
found in research publications. So as much as I pine for the good old
days the reality that I experience is that SEM is a minor methodology in
current biomedical research.
--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
4, 39 -- From Larry.Ackerman-at-ucsf.edu Thu Feb 5 16:21:43 2009
4, 39 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97])
4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15MLgMi029033
4, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:21:43 -0600
4, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with
4, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
4, 39 -- Thu, 05 Feb 2009 14:21:31 -0800
4, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
4, 39 -- X-AuditID: 403623d2-abe1ebb000007fb9-fb-498b82a9d657
4, 39 -- Received: from exbhmcb02.ucsfmedicalcenter.org (
4, 39 -- exbhmcb02.ucsfmedicalcenter.org [64.54.46.223]) by
4, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
4, 39 -- B18DC1426 for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:22:01
4, 39 -- -0800 (PST)
4, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
4, 39 -- exbhmcb02.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830);
4, 39 -- Thu, 5 Feb 2009 14:21:31 -0800
4, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
4, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 5 Feb
4, 39 -- 2009 14:21:31 -0800
4, 39 -- Message-ID: {498B666B.3080508-at-ucsf.edu}
4, 39 -- Date: Thu, 05 Feb 2009 14:21:31 -0800
4, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
4, 39 -- Reply-to: larry.ackerman-at-ucsf.edu
4, 39 -- Organization: UCSF, NeuroAnatomy
4, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
4, 39 -- MIME-Version: 1.0
4, 39 -- To: Microscopy-at-microscopy.com
4, 39 -- Subject: Re: [Microscopy] Current biological SEM
4, 39 -- References: {200902051657.n15GvPdW014913-at-ns.microscopy.com}
4, 39 -- In-Reply-To: {200902051657.n15GvPdW014913-at-ns.microscopy.com}
4, 39 -- X-OriginalArrivalTime: 05 Feb 2009 22:21:31.0459 (UTC)
4, 39 -- FILETIME=[18C27130:01C987E0]
4, 39 -- X-Brightmail-Tracker: AAAAAQNEgKM=
4, 39 -- X-WSS-ID: 6595B9E11SS1928881-01-01
4, 39 -- Content-Type: text/plain;
4, 39 -- charset=iso-8859-1;
4, 39 -- format=flowed
4, 39 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 5 Feb 2009 16:46:57 -0600
Subject: [Microscopy] Kodak, Ilford and Agfa Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Warren,

I have a concern regarding the separation of the iodoform and epoxy prior
to and following curing. Iodoform, being a small molecule, conceivable
could diffuse out of the epoxy resin into small voids faster than the
curing epoxy resin. If variable diffusion of iodoform and epoxy did occur,
might it not lead to poor infiltration of the sample? In your work, have
you seen any indication or are you aware of any instance in which iodoform
phase separates from the epoxy resin?

Thanks,

Gary M. Brown
Microscopy Specialist
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com

"Happiness equals reality minus expectations" Tom Magliozzi





wesaia-at-iastate
.edu
To
gary.m.brown-at-exxonmobil.com
01/28/09 02:27 cc
PM
Subject
[Microscopy] RE: Pre-embedding
Please respond staining of embedding media
to
wesaia-at-iastate
.edu











----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Years ago (1980s), I followed someone else's lead to dope epoxy resin
with iodoform to increase its average atomic number.

I was studying minerals in coal and standard epoxy resins provided
practically no contrast with coal in backscattered electron images. We
ended up dissolving about 15 wt.% iodoform in epoxy resin. We later
added the hardener and the epoxy behaved in much the same way as the
original two-part epoxy. That is, hardness and polymerization were
similar.

The resulting epoxy offered significant contrast with coal and allowed
us to proceed with automated image analyses.

Be advised that iodoform is rather nasty and needs to be handled with
care.

For what it's worth, I began working with iodoform shortly after the
Right-to-know laws were passed. Suppliers had recently started including
MSDSs with all their chemicals. I was still bemused that my
chemical-grade calcium carbonate "should be disposed of in an approved,
chemical-waste landfill" when my iodoform arrived. I had to do some more
of my own research to determine if iodoform was really as nasty as the
MSDS said or not. (It is.) Someone was crying wolf about the calcium
carbonate while a contractor was laying tons of the stuff just outside
our building as a base for the parking lot.

Warren S.

-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: Wednesday, January 28, 2009 1:03 PM
To: wesaia-at-iastate.edu

Hi Listers,
}  
} I have a lot of well aged photographic paper.  I don't
} know how old paper can be and still work, but I have one
} vintage box from 1975 and several from the 80's
}  
} If you miss the disco era and think you could use this
} paper let me know. 
}  
} My lab is being closed down for repairs starting Tuesday. 
} So it you act fast and pay to have it shipped I can send it
} you on Monday.  Otherwise you have to wait until the A/C
} repairs are completed (84 degree confocal rooms anyone) some
} time in March.

Paula

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
}  
}  






==============================Original Headers==============================
7, 27 -- From vapatpxs-at-yahoo.com Thu Feb 5 16:46:57 2009
7, 27 -- Received: from n77.bullet.mail.sp1.yahoo.com (n77.bullet.mail.sp1.yahoo.com [98.136.44.45])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n15MktgF028489
7, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:46:56 -0600
7, 27 -- Received: from [69.147.84.145] by n77.bullet.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000
7, 27 -- Received: from [69.147.84.116] by t8.bullet.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000
7, 27 -- Received: from [127.0.0.1] by omp208.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000
7, 27 -- X-Yahoo-Newman-Property: ymail-3
7, 27 -- X-Yahoo-Newman-Id: 697000.91640.bm-at-omp208.mail.sp1.yahoo.com
7, 27 -- Received: (qmail 40266 invoked by uid 60001); 5 Feb 2009 22:46:53 -0000
7, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 27 -- s=s1024; d=yahoo.com;
7, 27 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
7, 27 -- b=AZy5v26uwobEylQ/9SdM2Pit5pwdjQ/7PMpvQMoyKnRhPDMi05YUniHKstAiHy3UlzpQteQgUSrNGlRK9BEjSQEdai+lvG8acfLl4upUK8xK7VerCKzaP+zffMiT/vh0+OItFcIafvh+2cJNjiGRZLkSdlCjqDEDf65kMZzdii8=;
7, 27 -- X-YMail-OSG: xrWb3hQVM1kYUJj5Q.7IrtF9aUt3d7WYuSGPcUUcc6saJnf5piUHk5buHwDJPRS8Cl7Abzd87ITXkpJ.nStPftObYsbyReeJ2MbzJGBA9f9H2Btlr3p0IgWa3gmTziX0KSdfocWljHuL3sgsO1B_ESyjTD8-
7, 27 -- Received: from [152.132.10.194] by web46102.mail.sp1.yahoo.com via HTTP; Thu, 05 Feb 2009 14:46:53 PST
7, 27 -- X-Mailer: YahooMailWebService/0.7.260.1
7, 27 -- Date: Thu, 5 Feb 2009 14:46:53 -0800 (PST)
7, 27 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
7, 27 -- Reply-To: vapatpxs-at-yahoo.com
7, 27 -- Subject: Kodak, Ilford and Agfa Paper
7, 27 -- To: MSA BB {Microscopy-at-microscopy.com}
7, 27 -- MIME-Version: 1.0
7, 27 -- Content-Type: text/plain; charset=iso-8859-1
7, 27 -- Message-ID: {369567.39596.qm-at-web46102.mail.sp1.yahoo.com}
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15MktgF028489
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Thu, 5 Feb 2009 16:59:38 -0600
Subject: [Microscopy] Current Biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I did not notice any separation for my work. I was use fairly high beam
currents on an old W-gun SEM. I don't think I had the resolution to see
any separation. Maybe someone could detect that with these new SEMs. I
have not had occasion to go back and check.

If someone is interested in checking the stuff out, I still have some
polymerized pellets or could prepare more. I still have iodoform on
hand.

Warren S.

-----Original Message-----
X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com]
Sent: Thursday, February 05, 2009 4:28 PM
To: wesaia-at-iastate.edu


Years ago (1980s), I followed someone else's lead to dope epoxy resin
with iodoform to increase its average atomic number.

I was studying minerals in coal and standard epoxy resins provided
practically no contrast with coal in backscattered electron images. We
ended up dissolving about 15 wt.% iodoform in epoxy resin. We later
added the hardener and the epoxy behaved in much the same way as the
original two-part epoxy. That is, hardness and polymerization were
similar.

The resulting epoxy offered significant contrast with coal and allowed
us to proceed with automated image analyses.

Be advised that iodoform is rather nasty and needs to be handled with
care.

For what it's worth, I began working with iodoform shortly after the
Right-to-know laws were passed. Suppliers had recently started including
MSDSs with all their chemicals. I was still bemused that my
chemical-grade calcium carbonate "should be disposed of in an approved,
chemical-waste landfill" when my iodoform arrived. I had to do some more
of my own research to determine if iodoform was really as nasty as the
MSDS said or not. (It is.) Someone was crying wolf about the calcium
carbonate while a contractor was laying tons of the stuff just outside
our building as a base for the parking lot.

Warren S.

-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: Wednesday, January 28, 2009 1:03 PM
To: wesaia-at-iastate.edu

Hi Jon,

We have an old Hitachi S-520 SEM still operational.
Once or twice a year we are asked to look at paediatric hair specimens for
defects. Of course, babies can't tell what's wrong with them so looking at
hair samples can sometimes give you a clue as to what is the problem.
Ie, the hair defect may be one part of a wider syndrome.

Regards,

John Brealey

Senior Medical Scientist, Electron Microscopy Unit

T 08 8222 6612
F 08 8222 6425

www.sapathology.sa.gov.au

SA Pathology (TQEH)
Quality Pathology supporting Training and Research

This email may contain confidential information, which also may be legally
privileged. Only the intended recipient(s) may access, use, distribute or
copy this e-mail. If this e-mail is received in error, please inform the
sender by return e-mail and delete the original. If there are doubts about
the validity of this message, please contact the sender by telephone. It is
the recipient's responsibility to check the e-mail and any attached files
for viruses.



==============================Original Headers==============================
11, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 5 16:59:38 2009
11, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Mxapu015166
11, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:59:37 -0600
11, 27 -- X-IronPort-AV: E=Sophos;i="4.37,388,1231075800";
11, 27 -- d="scan'208";a="9016862"
11, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88])
11, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 06 Feb 2009 09:29:34 +1030
11, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
11, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id
11, 27 -- 8.1.263.0; Fri, 6 Feb 2009 09:30:20 +1030
11, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au
11, 27 -- (Postfix) with ESMTP id 2872534C13 for {Microscopy-at-microscopy.com} ; Fri, 6
11, 27 -- Feb 2009 09:29:34 +1030 (CST)
11, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
11, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
11, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
11, 27 -- Subject: Current Biological SEM
11, 27 -- Date: Fri, 6 Feb 2009 09:29:33 +1030
11, 27 -- Organization: IMVS
11, 27 -- Message-ID: {008d01c987e5$68ea35d0$5954150a-at-41347i}
11, 27 -- MIME-Version: 1.0
11, 27 -- Content-Type: text/plain; charset="us-ascii"
11, 27 -- Content-Transfer-Encoding: 7bit
11, 27 -- X-Mailer: Microsoft Office Outlook 11
11, 27 -- Thread-Index: AcmH5WjGJuYRPqLnSJqOKySzQVOteA==
11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Thu, 5 Feb 2009 17:02:00 -0600
Subject: [Microscopy] Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Larry et al,

As I replied to Jon (forgot to change the address to the server), we do a
fair bit of SEM, quite a bit of that is cryoSEM, and mostly of plants, with
a few insects now and then. The reason is to avoid extraction and other
artefacts - dehydration causes irreversible extraction of lots of soluble
material from tissues, esp. cell walls but also from plant vacuoles. We
also do analysis of mainly light element distribution and quantity in
specific tissues and cells. See recent review by McCully et al. (2009)
Functional Plant Biology 36:97 for more details. CryoSEM is also very good
for very soft material - for example, rotting tissues in which you want to
look at the disease organism in situ - which would fall apart with any other
preparation method.

As Tobias outlined, another couple of techniques we use much more these days
are environmental SEM, now that this technology is more mature and
reproducible, though still fiddly to get right at very low vacuum; and
FESEM.

The entomologists still look at bee's knees and fly eyes, though they tend
to look at details of the rear ends for taxonomic analysis.....

But I agree that with improvements to confocal imaging, and with
superresolution imaging almost consumer-ready - STED and cousins, light
microscopy will continue to explode, mainly because of the ability to
differentially tag cell components and cell functions in a huge variety of
ways.

cheers,
Rosemary


Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 6/02/09 9:28 AM, "larry.ackerman-at-ucsf.edu" {larry.ackerman-at-ucsf.edu}
wrote:


The perspective from an institution focused almost entirely on human
medical and related biological research studies puts SEM in the far
background. Whereas, there was once several SEM instruments on campus
there is now only one old, mostly inaccesible instrument in the Dental
School. I might hear an inquiry about SEM resources once a year and I
have to direct those investigators to a lab at another institution that
enjoys a full spectrum of departments and science fields. The vast
majority of funding and interest primarily focuses on genetic and
molecular questions. The SEM does not provide much useful data for these
studies though it is invaluable for descriptive illustrations. I think
this is supported by the dearth of SEM data in mamalian biology studies
in high profile publications such as Science, Nature and Cell. In
contrast light microscopy, particularly laser scanning confocal
microscopy, is booming. I estimate that there are over 30 heavily used
confocal systems at this university and light micrographs are commonly
found in research publications. So as much as I pine for the good old
days the reality that I experience is that SEM is a minor methodology in
current biomedical research.
--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400



==============================Original Headers==============================
17, 43 -- From prvs=Rosemary.White=2805e70b7-at-csiro.au Thu Feb 5 17:01:55 2009
17, 43 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37])
17, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15N1rqf021841
17, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 17:01:54 -0600
17, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
17, 43 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
17, 43 -- s=email; t=1233874915; x=1265410915;
17, 43 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
17, 43 -- mime-version:content-transfer-encoding:content-id:
17, 43 -- content-description:resent-date:resent-from:resent-sender:
17, 43 -- resent-to:resent-cc:resent-message-id:in-reply-to:
17, 43 -- references:list-id:list-help:list-unsubscribe:
17, 43 -- list-subscribe:list-post:list-owner:list-archive;
17, 43 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
17, 43 -- |Subject:=20Re:=20[Microscopy]=20Re:=20=20Current=20biolo
17, 43 -- gical=20SEM|Date:=20Fri,=206=20Feb=202009=2010:01:48=20+1
17, 43 -- 100|Message-ID:=20 {C5B1BB0C.32E0%rosemary.white-at-csiro.au}
17, 43 -- |To:=20 {Microscopy-at-microscopy.com} |MIME-Version:=201.0
17, 43 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200902
17, 43 -- 052228.n15MShna011475-at-ns.microscopy.com} ;
17, 43 -- bh=M96YpCybnweY1Zl6FfhdDehAki/vPyQtm2NJ3559F4A=;
17, 43 -- b=iGZMzw+dOJ3eg4JI9BTUaLX5bF1actAFs1tm03KdzUZM7Q2nmJZFczY7
17, 43 -- kyKXH05no3fKPLrO0kpcIwdXOqUS1GryjMirvgaqy8THoXpCdLy09PQ7a
17, 43 -- s09URxkvKzyagM7;
17, 43 -- X-IronPort-AV: E=Sophos;i="4.37,388,1231074000";
17, 43 -- d="scan'208";a="20394994"
17, 43 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
17, 43 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 06 Feb 2009 10:01:51 +1100
17, 43 -- Received: from [152.83.167.123] (152.83.167.123) by
17, 43 -- EXNSW-HTCA01.nexus.csiro.au (130.155.117.126) with Microsoft SMTP Server id
17, 43 -- 8.1.336.0; Fri, 6 Feb 2009 10:01:50 +1100
17, 43 -- User-Agent: Microsoft-Entourage/12.10.0.080409
17, 43 -- Date: Fri, 6 Feb 2009 10:01:48 +1100
17, 43 -- Subject: Re: [Microscopy] Re: Current biological SEM
17, 43 -- From: Rosemary White {rosemary.white-at-csiro.au}
17, 43 -- To: {Microscopy-at-microscopy.com}
17, 43 -- Message-ID: {C5B1BB0C.32E0%rosemary.white-at-csiro.au}
17, 43 -- Thread-Topic: [Microscopy] Re: Current biological SEM
17, 43 -- Thread-Index: AcmH4SC4qjBcFUzbTuChXgrfDhKARwABJhpb
17, 43 -- In-Reply-To: {200902052228.n15MShna011475-at-ns.microscopy.com}
17, 43 -- MIME-Version: 1.0
17, 43 -- Content-Type: text/plain; charset="US-ASCII"
17, 43 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Elliott-at-arizona.edu
Date: Thu, 5 Feb 2009 19:06:16 -0600
Subject: [Microscopy] Re: Scanner for Plate film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am currently using the Epson PhotoPerfection V700 Pro Scanner and
am very happy with it.
Good for 35mm slides also.
David


On Feb 5, 2009, at 2:55 PM, velliyka-at-umdnj.edu wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} I like to scan plate film (Philips EM300) , can you please suggest
} whic
} one is suitable for scaaning the EM films.
}
} Thank you very much for your help.
}
} V.Kabilan
} UMDNJ-NJDS
} Newark, USA
}
}
} ==============================Original
} Headers==============================
} 4, 26 -- From velliyka-at-umdnj.edu Thu Feb 5 15:52:55 2009
} 4, 26 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU
} [130.219.34.124])
} 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} id n15Lqs22022389
} 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 15:52:54
} -0600
} 4, 26 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
} 4, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 599E6A7B62
} 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009
} 16:52:52 -0500 (EST)
} 4, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
} 4, 26 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 6888BA7B28
} 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009
} 16:52:31 -0500 (EST)
} 4, 26 -- Received: from ([10.32.15.171])
} 4, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6189864;
} 4, 26 -- Thu, 05 Feb 2009 16:52:27 -0500
} 4, 26 -- MIME-version: 1.0
} 4, 26 -- Content-transfer-encoding: 7BIT
} 4, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
} 4, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
} 4, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging
} Server 6.3-6.03 (built
} 4, 26 -- Mar 14 2008; 32bit)) with ESMTP id
} {0KEM00EB04RFY9A0-at-umduwc02.umdnj.edu} for
} 4, 26 -- Microscopy-at-microscopy.com; Thu, 05 Feb 2009 16:52:27
} -0500 (EST)
} 4, 26 -- Message-id: {7fd8e9f8590e21c7591a62ceac910a1c-at-umdnj.edu}
} 4, 26 -- To: Microscopy-at-microscopy.com
} 4, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
} 4, 26 -- Subject: Scanner for Plate film
} 4, 26 -- Date: Thu, 05 Feb 2009 04:55:32 -0500
} 4, 26 -- X-Mailer: Apple Mail (2.624)
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
5, 22 -- From Elliott-at-arizona.edu Thu Feb 5 19:06:14 2009
5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1616E6a024843
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 5 Feb 2009 19:06:14 -0600
5, 22 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238])
5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id AB2639B7036
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 5 Feb 2009 18:06:12 -0700 (MST)
5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126])
5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 9939F9B6C98
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 5 Feb 2009 18:06:10 -0700 (MST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1)
5, 22 -- In-Reply-To: {200902052155.n15LtvSD026813-at-ns.microscopy.com}
5, 22 -- References: {200902052155.n15LtvSD026813-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
5, 22 -- Message-Id: {22308C5F-9DB9-4420-A869-011D47455C79-at-arizona.edu}
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- From: David Elliott {Elliott-at-arizona.edu}
5, 22 -- Subject: Re: [Microscopy] Scanner for Plate film
5, 22 -- Date: Thu, 5 Feb 2009 18:06:07 -0700
5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com}
5, 22 -- X-Mailer: Apple Mail (2.753.1)
5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: naomi_mccallum-at-health.qld.gov.au
Date: Fri, 6 Feb 2009 00:05:22 -0600
Subject: [Microscopy] Re: Current Biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon

We're in a similar situation to John. Most of our Diagnostic Pathology requests are for abnormalities associated with hair. Occasionally we also get some really interesting specimens for EDS for metals. E.g. Patients who self-prescribe homeopathic Silver solutions and end up with Agyria (the skin turns a silver blue colour...permanently!)

Regards


Naomi McCallum
Supervising Scientist
Electron Microscope Unit
Anatomical Pathology & Cytopathology
PATHOLOGY QUEENSLAND
Central Laboratory
RBWH

Ph +61 7 3636 8057
Fax +61 7 3636 8908
Naomi_McCallum-at-health.qld.gov.au


} } } {john.brealey-at-imvs.sa.gov.au} 6/02/2009 9:09 am } } }



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi Jon,

We have an old Hitachi S-520 SEM still operational.
Once or twice a year we are asked to look at paediatric hair specimens for
defects. Of course, babies can't tell what's wrong with them so looking at
hair samples can sometimes give you a clue as to what is the problem.
Ie, the hair defect may be one part of a wider syndrome.

Regards,

John Brealey

Senior Medical Scientist, Electron Microscopy Unit

T 08 8222 6612
F 08 8222 6425

www.sapathology.sa.gov.au

SA Pathology (TQEH)
Quality Pathology supporting Training and Research

This email may contain confidential information, which also may be legally
privileged. Only the intended recipient(s) may access, use, distribute or
copy this e-mail. If this e-mail is received in error, please inform the
sender by return e-mail and delete the original. If there are doubts about
the validity of this message, please contact the sender by telephone. It is
the recipient's responsibility to check the e-mail and any attached files
for viruses.



==============================Original Headers==============================
11, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 5 16:59:38 2009
11, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Mxapu015166
11, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:59:37 -0600
11, 27 -- X-IronPort-AV: E=Sophos;i="4.37,388,1231075800";
11, 27 -- d="scan'208";a="9016862"
11, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88])
11, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 06 Feb 2009 09:29:34 +1030
11, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
11, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id
11, 27 -- 8.1.263.0; Fri, 6 Feb 2009 09:30:20 +1030
11, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au
11, 27 -- (Postfix) with ESMTP id 2872534C13 for {Microscopy-at-microscopy.com} ; Fri, 6
11, 27 -- Feb 2009 09:29:34 +1030 (CST)
11, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
11, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
11, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
11, 27 -- Subject: Current Biological SEM
11, 27 -- Date: Fri, 6 Feb 2009 09:29:33 +1030
11, 27 -- Organization: IMVS
11, 27 -- Message-ID: {008d01c987e5$68ea35d0$5954150a-at-41347i}
11, 27 -- MIME-Version: 1.0
11, 27 -- Content-Type: text/plain; charset="us-ascii"
11, 27 -- Content-Transfer-Encoding: 7bit
11, 27 -- X-Mailer: Microsoft Office Outlook 11
11, 27 -- Thread-Index: AcmH5WjGJuYRPqLnSJqOKySzQVOteA==
11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================


********************************************************************************
This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.
**********************************************************************************



==============================Original Headers==============================
25, 27 -- From prvs=1288c7ae5c=naomi_mccallum-at-health.qld.gov.au Fri Feb 6 00:05:21 2009
25, 27 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114])
25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1665IPt018101
25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 00:05:20 -0600
25, 27 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53])
25, 27 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id n16653s9005548
25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:05:03 +1000
25, 27 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by
25, 27 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server)
25, 27 -- with ESMTP id
25, 27 -- {T8c58be87fdc0a803351874-at-gwd-mail06.remote.health.qld.gov.au} for
25, 27 -- {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:05:03 +1000
25, 27 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with
25, 27 -- Novell_GroupWise; Fri, 06 Feb 2009 16:05:03 +1000
25, 27 -- Message-Id: {498C5EC0.88BE.00AA.0-at-health.qld.gov.au}
25, 27 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
25, 27 -- Date: Fri, 06 Feb 2009 16:04:49 +1000
25, 27 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au}
25, 27 -- To: {Microscopy-at-microscopy.com}
25, 27 -- Subject: Re: [Microscopy] Current Biological SEM
25, 27 -- References: {200902052309.n15N9dP0015150-at-ns.microscopy.com}
25, 27 -- In-Reply-To: {200902052309.n15N9dP0015150-at-ns.microscopy.com}
25, 27 -- Mime-Version: 1.0
25, 27 -- Content-Type: text/plain; charset="us-ascii"
25, 27 -- Content-Disposition: inline
25, 27 -- Content-Transfer-Encoding: 8bit
25, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1665IPt018101
==============================End of - Headers==============================




From: smalinskas-at-yahoo.com
Date: Fri, 6 Feb 2009 07:50:19 -0600
Subject: [Microscopy] Re: Image Processing -- Ethics and Validity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe we had similar discussion on this topic some years back, as it related to evidence in litigation. I believe the concensus is that it is okay to adjust images for presentation or publication purposes as long as the data that is represented isn't significantly altered to show something that it should not be. This is why we adjust the contrast and brightness of digital images, and even back in the old days of print photography we "burned and dogged" the print. We did this to compensate for shortcomings of the equipment and technique used to gather documentation. In the case of legal matters, weight is placed on testimony of the expert witness, not on the picture that was taken.

The real value of the image is in the research, viewing, and interpretation of what the investigator saw before taking the image. The investigator (hopefully) knows the limitations of the equipment, tweaked the instrument to look at the image in different ways, and came up with his conclusion long before taking the picture.

I don't think any reasonable person would chastise somebody's photographic evidence or scientific documentation because the contrast was altered or an errant blemish was covered up in the name of producing an eye-pleasing presentation.

Stu Smalinskas, P.E.
Metallurgist
Plymouth, Michigan
SKF USA

--- On Thu, 2/5/09, sptinling-at-ucdavis.edu {sptinling-at-ucdavis.edu} wrote:

} From: sptinling-at-ucdavis.edu {sptinling-at-ucdavis.edu}
} Subject: [Microscopy] Image Processing -- Ethics and Validity
} To: smalinskas-at-yahoo.com
} Date: Thursday, February 5, 2009, 5:15 PM
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All,
} I am new here and this is my first response after
} reading Netnotes in Microscopy Today for
} years. Thanks for all the great
} information. Please pardon any initial unintended errors
} in protocol.
} I am an old dog learning new tricks so I would
} like members of this discussion to consider
} reconciling a couple of concepts. 1. Materials
} and Methods – complete reporting. 2. Significant
} figures (mathematically speaking).
} I was trained for EM and photography back in the
} days of glass plates and drum dryers with
} Pacasol. I now work in an age where we have
} discarded all our film photography materials and
} work with digital cameras and software. In the
} old days, dogging and burning were standard
} techniques in the dark room to get your EM
} picture to look perfect. No white spots from
} dust were allowed, and bits of stain in the lumen
} of an open perfused blood vessel were eliminated
} with a conveniently placed label and arrow. That
} was never considered as altering the significance
} of the data which the image conveyed and that was
} by an instructor who always stressed complete
} descriptions in the materials and methods section
} of everything that was done, all equipment used,
} etc. No one ever reported that the left corner
} of Fig.1 was dogged for 3 seconds while printing
} on grade 3 paper with the developer at 22 degrees
} centigrade. I have done enough EM to know that
} sometimes the picture you end up with has
} aesthetic defects. I am equally sure that most
} in not all the published EM (or LM, SEM, etc)
} images have that conveniently placed arrow or
} label somewhere. Should that have been reported
} then and or now? How is it any different if you
} remove what you know to be dirt or a stain
} particle from an image by any digital subtraction
} or addition or the rubber stamps method then by
} placing that convenient label or arrow over it
} and then merging the layers? You still have
} changed pixel values. I personally do not see
} the difference nor do I think it necessary to
} state in the figure legend or an appendix all the
} aesthetic alterations to an image. Also, I can
} not image a journal allowing you to. Even with
} online publishing I am constantly being told by
} the journals to make things shorter.
}
} As for the data contained in the digital image,
} the amount contained in individual pixel values
} and pixels per image goes way beyond the number
} of significant figures relevant to the data being
} presented by the image. And before you even get
} to Photoshop, the camera software lets you alter
} the image intensity, number of bits in an image
} (8, 12, 16) etc. To me it makes perfect sense to
} have a dark and light background image (as
} previously mentioned) and apply them to the image
} in question to eliminate defects in the optical
} path and report that in the M&M. However, if
} the point of the picture in question depends on
} the number of an individual pixel or the numbers
} of pixels in an image, then it makes sense to
} include any alterations in the M&M
} section. However, I suspect that for a very
} large percentage of the images published, a
} rubber stamped alteration of a capillary lumen
} for aesthetic purposes would be immaterial even
} though it changed the original values of the
} pixels in question. I would never consider that
} scientific fraud. Even when the pixels in
} question might have significance, when does the
} issue of reporting significant figures come in to
} the issue? I can accept that there are images
} produced where the data from the image are the
} pixel values. But, as I am mostly biologically
} oriented that does not happen very often to
} me. There are lots of papers out there in the
} early days of immunohistochemistry which tried to
} equate stain intensity (the number of a given
} pixel) with amount of pathology (numbers of
} pixels equal to or greater than a given pixel
} value per image with the right RGB or CMY or HIS,
} etc values) only to discover that the errors
} derived from trying to mix those two parameters
} yielded only false results. As my EM instructor
} always pointed out, everything we report is an
} “artifactâ€. So, if pixel values are that
} important, when do we apply the test of
} significant figures to the pixel data. If I
} publish 1 picture from the skin of 1 test animal
} showing “representative†damage to the basal
} lamina in an experimental group (lets say 15
} control and 15 experimental animals) then anyone
} who accepts my picture might further question the
} M&M section, for number of images taken per
} section, per sample, per animal and further ask
} if the data were derived from a sterologic
} process of sampling or whether I just got
} lucky. I rarely see any of this data
} reported. I realize as was said earlier that the
} day is coming when there will be enough space on
} the journal’s server for them to require that the
} original unaltered image be placed there along
} with the published one (in which you placed that
} convenient arrow) but then how do we know that an
} unaltered image was placed there in the first
} place? We do not -- we trust in the integrity
} within our community, and although fraud does
} occur is it at such a rate that it is really an
} issue? I suppose the well trained individual
} could download the original of a “questionableâ€
} published image and subject it to an analysis
} which would show pixel alterations and that would
} be good if one suspected deliberate fraud. My
} guess is that it would be a very rare thing. To
} close, I am a firm believer in complete M&M
} reporting and that would include how I took my
} image – camera, microscope, software, and
} techniques used, but I can not see burdening
} readers with the morally appropriate mea culpa
} that I typed a figure label or placed an arrow
} over an aesthetic defect and merged the layers to
} make a picture more aesthetically pleasing, or
} that I “rubberstamped†a piece of dirt. Thanks
} for considering these issues. Steve
}
} Steven P. Tinling Ph.D.
} Director of Research
} Otolaryngology Research Lab
} University of California, Davis
} 1515 Newton Ct., Rm. 209
} Davis, CA. 95616-4859
} Phone: 530-754-5045
} Fax: 530-754-5046
}
}
}
} ==============================Original
} Headers==============================
} 5, 22 -- From sptinling-at-ucdavis.edu Thu Feb 5 16:14:10
} 2009
} 5, 22 -- Received: from warsaw.ucdavis.edu
} (warsaw.ucdavis.edu [128.120.32.41])
} 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n15ME9O8011215
} 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb
} 2009 16:14:10 -0600
} 5, 22 -- Received: from MOTO-2951.ucdavis.edu
} ([128.120.223.66])
} 5, 22 -- (authenticated bits=0)
} 5, 22 -- by warsaw.ucdavis.edu
} (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n15ME3sK026356
} 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA
} bits=256 verify=NO)
} 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb
} 2009 14:14:04 -0800 (PST)
} 5, 22 -- Message-Id:
} {200902052214.n15ME3sK026356-at-warsaw.ucdavis.edu}
} 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 5, 22 -- Date: Thu, 05 Feb 2009 14:14:28 -0800
} 5, 22 -- To: Microscopy-at-Microscopy.Com
} 5, 22 -- From: Steve Tinling {sptinling-at-ucdavis.edu}
} 5, 22 -- Subject: Image Processing -- Ethics and Validity
} 5, 22 -- Mime-Version: 1.0
} 5, 22 -- Content-Type: text/plain;
} charset="iso-8859-1"; format=flowed
} 5, 22 -- X-Virus-Scanned: ClamAV version 0.93.3,
} clamav-milter version 0.93.3 on av5
} 5, 22 -- X-Virus-Status: Clean
} 5, 22 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41
} 5, 22 -- Content-Transfer-Encoding: 8bit
} 5, 22 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n15ME9O8011215
} ==============================End of -
} Headers==============================





==============================Original Headers==============================
9, 23 -- From smalinskas-at-yahoo.com Fri Feb 6 07:50:19 2009
9, 23 -- Received: from web34405.mail.mud.yahoo.com (web34405.mail.mud.yahoo.com [66.163.178.154])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16DoJ7D005023
9, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 6 Feb 2009 07:50:19 -0600
9, 23 -- Received: (qmail 32083 invoked by uid 60001); 6 Feb 2009 13:50:18 -0000
9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
9, 23 -- s=s1024; d=yahoo.com;
9, 23 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
9, 23 -- b=jQMlXOx1tr350MM3DeHYqfMh4xyxzHhsHYJHkn8472pz5jm2azP+6NIcq98D+VQkm8IGtWRB3SPrxy7Y1G1vUcxkDpyhtHzrmFCpP7+MUireuFEqkhcp5GI3B2NtzFl7rhW59LS4/xlm81dOGwVR343EgZTgV6rcSdmeQonsWEE=;
9, 23 -- X-YMail-OSG: M69shaoVM1ko3RemqbTIgpXlrpu2CHDz88FYhvYt.jTt_XrOUnsdTrSgX4jbFHCKqIrLxSvF6AsqMpFMqsNUcR09itz.p3kbCSFps0lkAS9Md0BmbpwQqzyJ28boR3aEfI3t4fsZv75hJ4x32F4lvLike59T8HyuCxokbQ_Me.QlG9jQtdgiGT0PBxWgLNwXbC4dOcT5_SXH3Om6iGKuqH0ug4Kzl3s-
9, 23 -- Received: from [141.151.33.213] by web34405.mail.mud.yahoo.com via HTTP; Fri, 06 Feb 2009 05:50:18 PST
9, 23 -- X-Mailer: YahooMailWebService/0.7.260.1
9, 23 -- Date: Fri, 6 Feb 2009 05:50:18 -0800 (PST)
9, 23 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 23 -- Reply-To: smalinskas-at-yahoo.com
9, 23 -- Subject: Re: [Microscopy] Image Processing -- Ethics and Validity
9, 23 -- To: sptinling-at-ucdavis.edu, microscopy-at-ns.microscopy.com
9, 23 -- In-Reply-To: {200902052215.n15MFm6M015702-at-ns.microscopy.com}
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain; charset=utf-8
9, 23 -- Message-ID: {559072.30236.qm-at-web34405.mail.mud.yahoo.com}
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16DoJ7D005023
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Fri, 6 Feb 2009 07:54:21 -0600
Subject: [Microscopy] Re:Scanner for plate film: Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Good morning, I like to thank every one who respond to me and It
would be a great help. I am new to this forum and it is wonderful and
supportive forum for microscopy . I would like to introduce myself as
my name is Kabilan V.Gounder working in UMDNJ-NJDS in New Jersey and i
am running an EM facility in our school. I am happy to join in this
group. Have nice day.

Sincerely
V.Kabilan


==============================Original Headers==============================
3, 26 -- From velliyka-at-umdnj.edu Fri Feb 6 07:54:21 2009
3, 26 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16DsLG2010495
3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 07:54:21 -0600
3, 26 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1])
3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 1AEDB4BE60
3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:54:21 -0500 (EST)
3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
3, 26 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 6D47A4BEA0
3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:54:06 -0500 (EST)
3, 26 -- Received: from ([10.32.15.171])
3, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6261191;
3, 26 -- Fri, 06 Feb 2009 08:54:01 -0500
3, 26 -- MIME-version: 1.0
3, 26 -- Content-transfer-encoding: 7BIT
3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
3, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
3, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KEN00I1TDA1VT70-at-umduwc02.umdnj.edu} for
3, 26 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 08:54:01 -0500 (EST)
3, 26 -- Message-id: {c2866f9cc5838564f89ef176565b8432-at-umdnj.edu}
3, 26 -- To: microscopy-at-microscopy.com
3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
3, 26 -- Subject: Re:Scanner for plate film: Thanks
3, 26 -- Date: Thu, 05 Feb 2009 20:57:06 -0500
3, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Fri, 6 Feb 2009 08:39:54 -0600
Subject: [Microscopy] RE: Scanner for Plate film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I followed this thread on the list with great interest as I was setting up
our new integrated imaging facility. I also chose the Epson perfection 750
and we're very satisfied with the results

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca

-----Original Message-----
X-from: Robert.Zonis-at-Sanford.com [mailto:Robert.Zonis-at-Sanford.com]
Sent: Thursday, February 05, 2009 5:18 PM
To: rjharris-at-uwo.ca

I prefer the Epson Perfection V750-M Pro Scanner. It costs around $700,
but the extra money is worth it for the extra software and calibration
standard that is included. It makes a pretty decent stand-in for a
stereoscopic microscope, too.

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.

-----Original Message-----


} I like to scan plate film (Philips EM300) , can you please suggest whic

} one is suitable for scaaning the EM films.
}
} Thank you very much for your help.
}
} V.Kabilan
} UMDNJ-NJDS
} Newark, USA



This message may contain information that is confidential and/or protected
by law. If the reader of this message is not the intended recipient, you are
hereby notified that any dissemination, distribution, copying or
communication of this message is strictly prohibited. If you have received
this communication in error, please contact the sender immediately and
delete the message. Please note that although we will take all commercially
reasonable efforts to prevent viruses from being transmitted from our
systems, it is the responsibility of the recipient to check for and prevent
adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
11, 34 -- From Robert.Zonis-at-Sanford.com Thu Feb 5 16:09:50 2009
11, 34 -- Received: from mail142.messagelabs.com (mail142.messagelabs.com
[216.82.249.99])
11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n15M9nuW004441
11, 34 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:09:50
-0600
11, 34 -- X-VirusChecked: Checked
11, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
11, 34 -- X-Msg-Ref:
server-8.tower-142.messagelabs.com!1233871787!65085875!1
11, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
11, 34 -- X-Originating-IP: [198.176.16.25]
11, 34 -- Received: (qmail 12669 invoked from network); 5 Feb 2009 22:09:47
-0000
11, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com)
(198.176.16.25)
11, 34 -- by server-8.tower-142.messagelabs.com with RC4-SHA encrypted
SMTP; 5 Feb 2009 22:09:47 -0000
11, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64])
by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
11, 34 -- Thu, 5 Feb 2009 16:09:46 -0600
11, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 34 -- Content-class: urn:content-classes:message
11, 34 -- MIME-Version: 1.0
11, 34 -- Content-Type: text/plain;
11, 34 -- charset="us-ascii"
11, 34 -- Subject: RE: [Microscopy] Scanner for Plate film
11, 34 -- Date: Thu, 5 Feb 2009 16:07:49 -0600
11, 34 -- Message-ID:
{66260BA266051B4FA0EC9EA3B33E6A9401950A46-at-naoaksasebe02.nr.ad.newellco.com}
11, 34 -- In-Reply-To: {200902052159.n15LxEks031584-at-ns.microscopy.com}
11, 34 -- X-MS-Has-Attach:
11, 34 -- X-MS-TNEF-Correlator:
11, 34 -- Thread-Topic: [Microscopy] Scanner for Plate film
11, 34 -- Thread-Index: AcmH3P7d8bcKCLdLQA+8rheHYN/2EQAAGMCA
11, 34 -- References: {200902052159.n15LxEks031584-at-ns.microscopy.com}
11, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
11, 34 -- To: {velliyka-at-umdnj.edu}
11, 34 -- Cc: {microscopy-at-microscopy.com}
11, 34 -- X-OriginalArrivalTime: 05 Feb 2009 22:09:46.0856 (UTC)
FILETIME=[74C88E80:01C987DE]
11, 34 -- Content-Transfer-Encoding: 8bit
11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n15M9nuW004441
==============================End of - Headers==============================



==============================Original Headers==============================
21, 28 -- From rjharris-at-uwo.ca Fri Feb 6 08:39:53 2009
21, 28 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Edpwg001996
21, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:39:52 -0600
21, 28 -- MIME-version: 1.0
21, 28 -- Content-type: text/plain; charset=iso-8859-1
21, 28 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41])
21, 28 -- by zeppo.mail.uwo.pri
21, 28 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
21, 28 -- with ESMTP id {0KEN00B6AFEEBIH0-at-zeppo.mail.uwo.pri} for
21, 28 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 09:39:50 -0500 (EST)
21, 28 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
21, 28 -- by zeppo.mail.uwo.pri
21, 28 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
21, 28 -- with ESMTPSA id {0KEN001DEFEEB640-at-zeppo.mail.uwo.pri} for
21, 28 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 09:39:50 -0500 (EST)
21, 28 -- From: Richard Harris {rjharris-at-uwo.ca}
21, 28 -- To: Microscopy List {microscopy-at-microscopy.com}
21, 28 -- References: {200902052217.n15MHrsT021497-at-ns.microscopy.com}
21, 28 -- In-reply-to: {200902052217.n15MHrsT021497-at-ns.microscopy.com}
21, 28 -- Subject: RE: [Microscopy] RE: Scanner for Plate film
21, 28 -- Date: Fri, 06 Feb 2009 09:39:47 -0500
21, 28 -- Message-id: {003001c98868$c4d72460$4e856d20$-at-ca}
21, 28 -- X-Mailer: Microsoft Office Outlook 12.0
21, 28 -- Thread-index: AcmH35tQiGhrYILiR8KQNmcC3uzwYgAiNdzQ
21, 28 -- Content-language: en-us
21, 28 -- Content-Transfer-Encoding: 8bit
21, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16Edpwg001996
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 6 Feb 2009 09:28:37 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Weellll... I have to agree that SEM isn't used much in biomedical
research, but disagree about why.
SEM is not used much in biological work, biomedical in particular,
but I think it is used more than indicated here, and that it is under
used.
I worked on many SEM biomedical/bioengineering projects at UW-Madison
involving cultured cells, biofllms in catheters, implanted medical
devices, bone fractures, and others.
And using gold-conjugated antibodies to study cell-surface receptors.
SEM is a much better method for this than TEM or confocal, as the
entire cell surface can be sampled at high resolution, instead of
just very thin cross-sectional slices that could easily miss the
receptors, or instead of at light microscope resolution. Studies like
this can raise and answer questions like: are the receptors
distributed at random on the cell surface or in patterns? Is there
any relation between receptor distribution and cell surface
structures like ruffles, etc.?
And so on.
There are many biomedical molecular/genetic questions that could be
addressed with SEM, there just are few people thinking of them. The
problem isn't "how useful is the technique", but how people are
thinking and how much they know about what techniques are available.
Most molecular/genetic people know little or nothing of microscopy,
or EM, much less SEM, so they never consider them useful techniques.
That just means they don't know much about the technique, not that
it's not useful.

Phl

} The perspective from an institution focused almost entirely on human
} medical and related biological research studies puts SEM in the far
} background. Whereas, there was once several SEM instruments on campus
} there is now only one old, mostly inaccesible instrument in the Dental
} School. I might hear an inquiry about SEM resources once a year and I
} have to direct those investigators to a lab at another institution that
} enjoys a full spectrum of departments and science fields. The vast
} majority of funding and interest primarily focuses on genetic and
} molecular questions. The SEM does not provide much useful data for these
} studies though it is invaluable for descriptive illustrations. I think
} this is supported by the dearth of SEM data in mamalian biology studies
} in high profile publications such as Science, Nature and Cell. In
} contrast light microscopy, particularly laser scanning confocal
} microscopy, is booming. I estimate that there are over 30 heavily used
} confocal systems at this university and light micrographs are commonly
} found in research publications. So as much as I pine for the good old
} days the reality that I experience is that SEM is a minor methodology in
} current biomedical research.
} --
} Larry Ackerman, Specialist
} UCSF, Dept. of Anatomy, Rm S1347
} 513 Parnassus Ave., Box 0452
} San Francisco, CA 94143
}
} larry.ackerman-at-ucsf.edu
}
} 415-476-4400
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
3, 25 -- From oshel1pe-at-cmich.edu Fri Feb 6 09:28:37 2009
3, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FSatC017446
3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:28:36 -0600
3, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
3, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n16FSY48026412
3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 10:28:35 -0500
3, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
3, 25 -- Fri, 6 Feb 2009 10:28:03 -0500
3, 25 -- Mime-Version: 1.0
3, 25 -- Message-Id: {f0624080dc5b204816b90-at-[141.209.160.249]}
3, 25 -- In-Reply-To: {200902052225.n15MPXlK005895-at-ns.microscopy.com}
3, 25 -- References: {200902052225.n15MPXlK005895-at-ns.microscopy.com}
3, 25 -- Date: Fri, 6 Feb 2009 10:28:00 -0500
3, 25 -- To: Microscopy-at-microscopy.com
3, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 25 -- Subject: [Microscopy] Re: Current biological SEM
3, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 25 -- X-OriginalArrivalTime: 06 Feb 2009 15:28:03.0112 (UTC) FILETIME=[803EDA80:01C9886F]
3, 25 -- X-Canit-CHI2: 0.00
3, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
3, 25 -- X-CanItPRO-Stream: default
3, 25 -- X-Canit-Stats-ID: 8545392 - d52e9df7ecec
3, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 6 Feb 2009 09:43:05 -0600
Subject: [Microscopy] Re: Current biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with Phil on this, and it may be partially due to the EM
community not reaching out to researchers with information about the
capabilities of our techniques.

It's easy, for us at least, to fall into the routine of just reacting to
the researchers coming through the door with their projects and a
preconceived idea of the best way to approach them. We often have
clients come in with completely inappropriate protocols that they want
us to follow, simply because they have seen them in a journal article
and believe that they are universally applicable. Quite often they may
be completely uninformed about other, more appropriate, techniques.

Being a bit more proactive in getting information out in the form of
workshops, brown bag lectures, newsletters, etc., might do wonders in
renewing interest in things like use of SEM technologies in biological
research.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Friday, February 06, 2009 9:30 AM
To: Tindall, Randy D.

Weellll... I have to agree that SEM isn't used much in biomedical
research, but disagree about why.
SEM is not used much in biological work, biomedical in particular,
but I think it is used more than indicated here, and that it is under
used.
I worked on many SEM biomedical/bioengineering projects at UW-Madison
involving cultured cells, biofllms in catheters, implanted medical
devices, bone fractures, and others.
And using gold-conjugated antibodies to study cell-surface receptors.
SEM is a much better method for this than TEM or confocal, as the
entire cell surface can be sampled at high resolution, instead of
just very thin cross-sectional slices that could easily miss the
receptors, or instead of at light microscope resolution. Studies like
this can raise and answer questions like: are the receptors
distributed at random on the cell surface or in patterns? Is there
any relation between receptor distribution and cell surface
structures like ruffles, etc.?
And so on.
There are many biomedical molecular/genetic questions that could be
addressed with SEM, there just are few people thinking of them. The
problem isn't "how useful is the technique", but how people are
thinking and how much they know about what techniques are available.
Most molecular/genetic people know little or nothing of microscopy,
or EM, much less SEM, so they never consider them useful techniques.
That just means they don't know much about the technique, not that
it's not useful.

Phl

} The perspective from an institution focused almost entirely on human
} medical and related biological research studies puts SEM in the far
} background. Whereas, there was once several SEM instruments on campus
} there is now only one old, mostly inaccesible instrument in the Dental
} School. I might hear an inquiry about SEM resources once a year and I
} have to direct those investigators to a lab at another institution that
} enjoys a full spectrum of departments and science fields. The vast
} majority of funding and interest primarily focuses on genetic and
} molecular questions. The SEM does not provide much useful data for
these
} studies though it is invaluable for descriptive illustrations. I think
} this is supported by the dearth of SEM data in mamalian biology studies
} in high profile publications such as Science, Nature and Cell. In
} contrast light microscopy, particularly laser scanning confocal
} microscopy, is booming. I estimate that there are over 30 heavily used
} confocal systems at this university and light micrographs are commonly
} found in research publications. So as much as I pine for the good old
} days the reality that I experience is that SEM is a minor methodology
in
} current biomedical research.
} --
} Larry Ackerman, Specialist
} UCSF, Dept. of Anatomy, Rm S1347
} 513 Parnassus Ave., Box 0452
} San Francisco, CA 94143
}
} larry.ackerman-at-ucsf.edu
}
} 415-476-4400
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original
Headers==============================
3, 25 -- From oshel1pe-at-cmich.edu Fri Feb 6 09:28:37 2009
3, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n16FSatC017446
3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009
09:28:36 -0600
3, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
3, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id
n16FSY48026412
3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009
10:28:35 -0500
3, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by
egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
3, 25 -- Fri, 6 Feb 2009 10:28:03 -0500
3, 25 -- Mime-Version: 1.0
3, 25 -- Message-Id: {f0624080dc5b204816b90-at-[141.209.160.249]}
3, 25 -- In-Reply-To: {200902052225.n15MPXlK005895-at-ns.microscopy.com}
3, 25 -- References: {200902052225.n15MPXlK005895-at-ns.microscopy.com}
3, 25 -- Date: Fri, 6 Feb 2009 10:28:00 -0500
3, 25 -- To: Microscopy-at-microscopy.com
3, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 25 -- Subject: [Microscopy] Re: Current biological SEM
3, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 25 -- X-OriginalArrivalTime: 06 Feb 2009 15:28:03.0112 (UTC)
FILETIME=[803EDA80:01C9886F]
3, 25 -- X-Canit-CHI2: 0.00
3, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00]
L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
3, 25 -- X-CanItPRO-Stream: default
3, 25 -- X-Canit-Stats-ID: 8545392 - d52e9df7ecec
3, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on
141.209.20.25
==============================End of -
Headers==============================


==============================Original Headers==============================
17, 30 -- From TindallR-at-missouri.edu Fri Feb 6 09:43:05 2009
17, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Fh4E7031692
17, 30 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:43:04 -0600
17, 30 -- X-IronPort-Anti-Spam-Filtered: true
17, 30 -- X-IronPort-Anti-Spam-Result: ApoEAFPpi0nRauUo/2dsb2JhbADBGgEJhSGIR4JhgTUGgjODIg
17, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
17, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 06 Feb 2009 09:43:04 -0600
17, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
17, 30 -- Fri, 6 Feb 2009 09:43:04 -0600
17, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 30 -- Content-class: urn:content-classes:message
17, 30 -- MIME-Version: 1.0
17, 30 -- Content-Type: text/plain;
17, 30 -- charset="us-ascii"
17, 30 -- Subject: RE: [Microscopy] Re: Current biological SEM
17, 30 -- Date: Fri, 6 Feb 2009 09:43:02 -0600
17, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D9D-at-UM-XMAIL08.um.umsystem.edu}
17, 30 -- In-Reply-To: {200902061529.n16FTl07019090-at-ns.microscopy.com}
17, 30 -- X-MS-Has-Attach:
17, 30 -- X-MS-TNEF-Correlator:
17, 30 -- Thread-Topic: [Microscopy] Re: Current biological SEM
17, 30 -- Thread-Index: AcmIb79qbAkuuFpFRZuZFqNg7e4BJwAAElvg
17, 30 -- References: {200902061529.n16FTl07019090-at-ns.microscopy.com}
17, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
17, 30 -- To: {oshel1pe-at-cmich.edu}
17, 30 -- Cc: {microscopy-at-microscopy.com}
17, 30 -- X-OriginalArrivalTime: 06 Feb 2009 15:43:04.0472 (UTC) FILETIME=[997F7980:01C98871]
17, 30 -- Content-Transfer-Encoding: 8bit
17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16Fh4E7031692
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Fri, 6 Feb 2009 14:43:02 -0600
Subject: [Microscopy] Nikon coolpix and Image J

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

While brightness, contrast and gamma are fine to adjust. I would not
feel comfortable in modifying the image to "cover up" a
blemish. Assuming you are using a rubber stamp tool or something
similar, you are modifying the information in the original
image. You are then changing the information content of the
picture. In my (not so humble) opinion, this is a dangerous step to take.

Cheers,
Henk



At 08:52 AM 02/06/09, you wrote:


} {...snip...}
} I don't think any reasonable person would chastise somebody's
} photographic evidence or scientific documentation because the
} contrast was altered or an errant blemish was covered up in the name
} of producing an eye-pleasing presentation.
}
} Stu Smalinskas, P.E.
} Metallurgist
} Plymouth, Michigan
} SKF USA

{...snip...}


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009
12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FlnoU009705
12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:47:49 -0600
12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST)
12, 26 -- Received: from HOC1.ecr6.ohio-state.edu
12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
12, 26 -- (PMDF V6.3-x18 #31556)
12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri,
12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST)
12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500
12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and Validity
12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
12, 26 -- To: smalinskas-at-yahoo.com
12, 26 -- Cc: Microscopy-at-microscopy.com
12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu}
12, 26 -- MIME-version: 1.0
12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
==============================End of - Headers==============================

From primasa-at-silosprima.com.ar Fri Feb 6 11:27:25 2009
Return-Path: {primasa-at-silosprima.com.ar}
Received: from google.com (78-141-65-141.minet.sk [78.141.65.141] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16HROAE000333
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 6 Feb 2009 11:27:25 -0600
Received: from [80.99.105.175] (HELO google.com)
by striped-sap.cn; Fri, 6 Feb 2009 18:27:06 +0100

Dear Listers
Has anyone used Image J and a Nikon Coolpix to acquire images to a
computer??? I am looking for an inexpensive image capture solution for my
Microscopy, Imaging and Analysis course and this combo (if it works) looks
like it would fit the bill...

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca





==============================Original Headers==============================
7, 27 -- From rjharris-at-uwo.ca Fri Feb 6 14:43:00 2009
7, 27 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16KgxXN020552
7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:42:59 -0600
7, 27 -- MIME-version: 1.0
7, 27 -- Content-type: text/plain; charset=iso-8859-1
7, 27 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39])
7, 27 -- by harpo.mail.uwo.pri
7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 27 -- with ESMTP id {0KEN00JJ3W7NJNE0-at-harpo.mail.uwo.pri} for
7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST)
7, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
7, 27 -- by harpo.mail.uwo.pri
7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 27 -- with ESMTPSA id {0KEN00FUMW7NCN00-at-harpo.mail.uwo.pri} for
7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST)
7, 27 -- From: Richard Harris {rjharris-at-uwo.ca}
7, 27 -- To: Microscopy List Server {microscopy-at-microscopy.com} ,
7, 27 -- MSA Listserver {Microscopy-at-MSA.Microscopy.Com}
7, 27 -- Subject: Nikon coolpix and Image J
7, 27 -- Date: Fri, 06 Feb 2009 15:43:01 -0500
7, 27 -- Message-id: {003401c9889b$80b14760$8213d620$-at-ca}
7, 27 -- X-Mailer: Microsoft Office Outlook 12.0
7, 27 -- Thread-index: AcmIm4CIq+YuzxOzQbugKKhlmR1E0g==
7, 27 -- Content-language: en-us
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16KgxXN020552
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Fri, 6 Feb 2009 14:53:08 -0600
Subject: [Microscopy] RE: Philips EM300 plate film: no image on negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I just changed the filament for Philips EM300 and saturated, the
illumination looks good and I observe the samples. But the problem is
when I develop the negatives, it looks empty. There was no image,
Please let me know what is the problem and why the film is empty.
Thanks for your help.

Kabilan
UMDNJ-NJDS
Newark, NJ, USA


==============================Original Headers==============================
4, 28 -- From velliyka-at-umdnj.edu Fri Feb 6 14:53:08 2009
4, 28 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125])
4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16Kr7Yo001511
4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:53:08 -0600
4, 28 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1])
4, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id DE1A24BE74
4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:53:06 -0500 (EST)
4, 28 -- Received: from umdnj.edu (unknown [10.32.15.102])
4, 28 -- by zix02.umdnj.edu (Proprietary) with ESMTP id E3D1C4BE7C
4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:51:10 -0500 (EST)
4, 28 -- Received: from ([10.32.15.171])
4, 28 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.6590949;
4, 28 -- Fri, 06 Feb 2009 15:50:41 -0500
4, 28 -- MIME-version: 1.0
4, 28 -- Content-transfer-encoding: 7BIT
4, 28 -- Content-type: text/plain; charset=US-ASCII; format=flowed
4, 28 -- Received: from [10.4.62.229] ([10.32.15.102])
4, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
4, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KEN00KD5WKHMN20-at-umduwc02.umdnj.edu} for
4, 28 -- Microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:50:41 -0500 (EST)
4, 28 -- In-reply-to: {20050228.151138.2728.0.fgask99200-at-juno.com}
4, 28 -- References: {20050228.151138.2728.0.fgask99200-at-juno.com}
4, 28 -- Message-id: {a49997ced5731f407b847097f4ccecf1-at-umdnj.edu}
4, 28 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
4, 28 -- Subject: RE: Philips EM300 plate film: no image on negatives
4, 28 -- Date: Fri, 06 Feb 2009 03:53:48 -0500
4, 28 -- To: Microscopy-at-microscopy.com
4, 28 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: jsiegmund-at-7thwavelabs.com
Date: Fri, 6 Feb 2009 15:10:08 -0600
Subject: [Microscopy] Nikon coolpix and Image J

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Rick,
There is a Twain plugin for ImageJ, that should work with the most common interfaces (...is there a Nikon Twain Driver?). Also, if you save your images to the harddrive, like with Nikon Transfer or your camera software, you can import and batch import the images into ImageJ.

Joachim



-----Original Message-----
X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
Sent: Friday, February 06, 2009 2:53 PM
To: Joachim Siegmund

Dear Listers
Has anyone used Image J and a Nikon Coolpix to acquire images to a
computer??? I am looking for an inexpensive image capture solution for my
Microscopy, Imaging and Analysis course and this combo (if it works) looks
like it would fit the bill...

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca





==============================Original Headers==============================
7, 27 -- From rjharris-at-uwo.ca Fri Feb 6 14:43:00 2009
7, 27 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16KgxXN020552
7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:42:59 -0600
7, 27 -- MIME-version: 1.0
7, 27 -- Content-type: text/plain; charset=iso-8859-1
7, 27 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39])
7, 27 -- by harpo.mail.uwo.pri
7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 27 -- with ESMTP id {0KEN00JJ3W7NJNE0-at-harpo.mail.uwo.pri} for
7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST)
7, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
7, 27 -- by harpo.mail.uwo.pri
7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 27 -- with ESMTPSA id {0KEN00FUMW7NCN00-at-harpo.mail.uwo.pri} for
7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST)
7, 27 -- From: Richard Harris {rjharris-at-uwo.ca}
7, 27 -- To: Microscopy List Server {microscopy-at-microscopy.com} ,
7, 27 -- MSA Listserver {Microscopy-at-MSA.Microscopy.Com}
7, 27 -- Subject: Nikon coolpix and Image J
7, 27 -- Date: Fri, 06 Feb 2009 15:43:01 -0500
7, 27 -- Message-id: {003401c9889b$80b14760$8213d620$-at-ca}
7, 27 -- X-Mailer: Microsoft Office Outlook 12.0
7, 27 -- Thread-index: AcmIm4CIq+YuzxOzQbugKKhlmR1E0g==
7, 27 -- Content-language: en-us
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16KgxXN020552
==============================End of - Headers==============================

This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer


==============================Original Headers==============================
20, 29 -- From jsiegmund-at-7thwavelabs.com Fri Feb 6 15:10:08 2009
20, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136])
20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16LA8c3016319
20, 29 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:10:08 -0600
20, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1])
20, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 0480839800A;
20, 29 -- Fri, 6 Feb 2009 15:10:08 -0600 (CST)
20, 29 -- X-AuditID: c0a80218-a303abb000000bbe-fc-498ca72f4a8b
20, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29])
20, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 8E5C3424013;
20, 29 -- Fri, 6 Feb 2009 15:10:07 -0600 (CST)
20, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 29 -- Content-class: urn:content-classes:message
20, 29 -- MIME-Version: 1.0
20, 29 -- Content-Type: text/plain;
20, 29 -- charset="iso-8859-1"
20, 29 -- Subject: RE: [Microscopy] Nikon coolpix and Image J
20, 29 -- Date: Fri, 6 Feb 2009 15:10:07 -0600
20, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A660B294-at-wave-mail.7thwave.local}
20, 29 -- X-MS-Has-Attach:
20, 29 -- X-MS-TNEF-Correlator:
20, 29 -- Thread-Topic: [Microscopy] Nikon coolpix and Image J
20, 29 -- Thread-Index: AcmInPMN3IKszekdQmOcIyZ8l0IoRwAAUX8g
20, 29 -- References: {200902062053.n16KrLgG002039-at-ns.microscopy.com}
20, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com}
20, 29 -- To: {rjharris-at-uwo.ca} , {microscopy-at-microscopy.com}
20, 29 -- X-Brightmail-Tracker: AAAAAA==
20, 29 -- Content-Transfer-Encoding: 8bit
20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16LA8c3016319
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Fri, 6 Feb 2009 15:23:42 -0600
Subject: [Microscopy] Re: Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Richie,

Based on our experience graphite rods do require about 18 to 20 amps
of current and a good vacuum.

We also think that the stub and the carbon point must be of the same
material for optimum results.

Regards,
John Arnott

Disclaimer: Ladd Research distributes EM supplies including carbon rods

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 02:25 PM 2/3/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
14, 27 -- From jd-at-laddresearch.com Fri Feb 6 15:23:42 2009
14, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16LNgaH030503
14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:23:42 -0600
14, 27 -- Received: from 1LVYAm-0001jM-WB by bean.electric.net with emc1-ok (Exim 4.69)
14, 27 -- (envelope-from {jd-at-laddresearch.com} )
14, 27 -- id 1LVYAn-0001kG-U3; Fri, 06 Feb 2009 13:23:41 -0800
14, 27 -- Received: by emcmailer; Fri, 06 Feb 2009 13:23:41 -0800
14, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
14, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256)
14, 27 -- (Exim 4.69)
14, 27 -- (envelope-from {jd-at-laddresearch.com} )
14, 27 -- id 1LVYAm-0001jM-WB; Fri, 06 Feb 2009 13:23:41 -0800
14, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 27 -- Date: Fri, 06 Feb 2009 16:23:38 -0500
14, 27 -- To: r.sims-at-auckland.ac.nz
14, 27 -- From: jd {jd-at-laddresearch.com}
14, 27 -- Subject: Re: [Microscopy] Carbon Rods
14, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
14, 27 -- In-Reply-To: {200902031925.n13JPuFe021262-at-ns.microscopy.com}
14, 27 -- References: {200902031925.n13JPuFe021262-at-ns.microscopy.com}
14, 27 -- Mime-Version: 1.0
14, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
14, 27 -- X-Outbound-IP: 216.204.198.170
14, 27 -- X-Env-From: jd-at-laddresearch.com
14, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
14, 27 -- Message-Id: {E1LVYAn-0001kG-U3-at-bean.electric.net}
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 6 Feb 2009 15:55:41 -0600
Subject: [Microscopy] RE: Philips EM300 plate film: no image on negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} I just changed the filament for Philips EM300 and saturated, the
} illumination looks good and I observe the samples. But the problem is
} when I develop the negatives, it looks empty. There was no image,
} Please let me know what is the problem and why the film is empty.
} Thanks for your help.

Hello,

I'm sure that more than a few of us have had a similar experience.
Many different situations may cause this. Here are some things to
check:

1. Were the negatives put into the holder facing in the correct
direction (emulsion side up)? Recentley, someone remarked that the
negative sheets have been incorrectly notched so that (if you use the
notch for orientation), that may be the problem. Best way to check is
to take one holder into the light and see if the film was put in
properly.

2. Are the developer and fixer still active (not exhausted)? If in
doubt, prepare new solutions of everything.

3. Is the shutter working in the TEM? This is either a mechanical or
magnetic deflector. Best way to check is to keep the viewing screen
down and press the exposure button to see if illumination is
projected onto the viewing screen for the proper amount of time.

4. Is the exposure meter set properly in the TEM? If unsure, try
different sensitivity settings.

I'm sure others will have even more ideas, but this will get you started.

JB
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
11, 19 -- From bozzola-at-siu.edu Fri Feb 6 15:55:41 2009
11, 19 -- Received: from cstmta4.siu.edu (cstmta4.siu.edu [131.230.1.4])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Ltf4B012953
11, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:55:41 -0600
11, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
11, 19 -- by cstmta4.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n16Ltcp3001084
11, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:55:41 -0600 (CST)
11, 19 -- Mime-Version: 1.0
11, 19 -- Message-Id: {a06240803c5b258391c06-at-[131.230.177.136]}
11, 19 -- In-Reply-To: {200902062053.n16KrmwM003369-at-ns.microscopy.com}
11, 19 -- References: {200902062053.n16KrmwM003369-at-ns.microscopy.com}
11, 19 -- Date: Fri, 6 Feb 2009 15:55:37 -0600
11, 19 -- To: Microscopy-at-microscopy.com
11, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
11, 19 -- Subject: [Microscopy] RE: Philips EM300 plate film: no image on negatives
11, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
11, 19 -- X-Spam-Score: 0.00%
11, 19 -- X-MASF: 0.00%
11, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: ahlst007-at-umn.edu
Date: Fri, 6 Feb 2009 16:37:24 -0600
Subject: [Microscopy] Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richie, John, and listers,

I too, am looking for a new supply of 1/8"
carbon rods that don't require such high
currents, and therefore produce such high heat
loads, compared to the "older" ones that I have
left. These older ones only need about 16-18
amps to evaporate from a 3mm long x 0.8mm
diameter tip that I sharpen on it. These
low-current rods are a very dark black, and I
think a bit softer than the lighter gray silvery
ones that I also have and which take the high
currents to evaporate. This later type seems to
be all that's available out there now.

In fact, I contacted John at Ladd about a year
ago and he told me those black ones, which were
originally purchased from Ladd years ago (Ladd
part # 30267), are no longer available. I would
ask him again now what kind of carbon rods is he
talking about that only take 18-20 amps as he
mentioned?

I have a small stash of the black ones left, so
I hope to find some more before I run out. I did
try to contact the McMaster-Carr company about
their carbon rods. The smallest ones they have
are too big in diameter for our EM applications,
but I never got a response back from them.

Gib

--
Gilbert (Gib) Ahlstrand, Electron Microscopist,
Imaging Center, University of Minnesota
123 Snyder Hall
St. Paul, MN 55108
http://www.cbs.umn.edu/ic


jd-at-laddresearch.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Richie,
}
} Based on our experience graphite rods do require about 18 to 20 amps
} of current and a good vacuum.
}
} We also think that the stub and the carbon point must be of the same
} material for optimum results.
}
} Regards,
} John Arnott
}
} Disclaimer: Ladd Research distributes EM supplies including carbon rods
}
} Ladd Research
} 83 Holly Court
} Williston, VT 05495
}
} On-line Catalog: www.laddresearch.com
}
} Telephone: 1-802-658-4961 (anywhere)
} Toll Free 1-800-451-3406 (US)
} Fax: 1-802-660-8859
}
} e-mail: sales-at-laddresearch.com
}
} At 02:25 PM 2/3/2009, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi
} }
} } I've been having trouble with 3mm/1/8"carbon rods for my Edwards 306
} } coater since running
} } out of my stash of Union Carbide National Carbon Company
} } "Spectroscopic Electrodes" a
} } while ago.
} }
} } I don't have a reliably calibrated vacuum gauge in the 306, but I
} } suspect that the vacuum
} } achieved isn't that great, however, it's as good as it was when I
} } was getting great coating
} } from the previous rods.
} } I bought some "graphite" rods from one supplier, but they needed a
} } higher current and
} } temperature than the 306 could achieve. I then bought some
} } "amorphous carbon" rods, but
} } the success rate isn't very high, there just doesn't seem to be much
} } coating produced.
} }
} } As I understand it, all rods contain a greater or lesser ratio of
} } graphite and amorphous
} } carbon, and the greater the graphite content, the higher the
} } temperature required for coating.
} }
} } Does anyone know of a supplier of rods identical or similar to the
} } old Union Carbide National
} } Carbon Company ones?
} }
} } cheers
} } Ritchie
} }
} } --
} } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} } Microanalyst Fax : 64 9 3737435
} } Department of Geology email : r.sims-at-auckland.ac.nz
} } The University of Auckland
} } Private Bag 92019
} } Auckland
} } New Zealand

==============================Original Headers==============================
8, 22 -- From ahlst007-at-umn.edu Fri Feb 6 16:37:24 2009
8, 22 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16MbNV3028220
8, 22 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Feb 2009 16:37:23 -0600
8, 22 -- Received: from x-160-94-173-220.cbs.umn.edu (x-160-94-173-220.cbs.umn.edu [160.94.173.220])
8, 22 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
8, 22 -- Fri, 6 Feb 2009 16:37:17 -0600 (CST)
8, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-220.cbs.umn.edu [160.94.173.220] #+LO+TS+AU
8, 22 -- X-Umn-Classification: local
8, 22 -- Message-ID: {498CBBE3.9080603-at-umn.edu}
8, 22 -- Date: Fri, 06 Feb 2009 16:38:27 -0600
8, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu}
8, 22 -- Reply-To: ahlst007-at-umn.edu
8, 22 -- Organization: Imaging Center UM
8, 22 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
8, 22 -- MIME-Version: 1.0
8, 22 -- To: Microscopy-at-Microscopy.com
8, 22 -- Subject: Re: [Microscopy] Re: Carbon Rods
8, 22 -- References: {200902062125.n16LPxGR002965-at-ns.microscopy.com}
8, 22 -- In-Reply-To: {200902062125.n16LPxGR002965-at-ns.microscopy.com}
8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 22 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Sally.Stowe-at-anu.edu.au
Date: Fri, 6 Feb 2009 16:42:26 -0600
Subject: [Microscopy] Re: Image Processing -- Ethics and Validity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
I'm sure Henk is absolutely right. The line between science and advertising
or entertainment may be more accessible at a computer console than in a
darkroom, but it is no less clear.

I think we should be able to debate when it is permissible to cross that
line, recognising that sometimes it is almost impossible not to, rather
than attempting to re-position it to a more convenient location.

A label always obscures information, but after all it has to be somewhere.
Its placement may in some circumstances be dependent upon the conscience of
the author. Placing a lable over gold particles in an immunostained
"negative control" section would be clearly misleading, covering a small
"irrelevant" blemish would be seen as allowable by almost everyone.

Rubberstamping, however is intrinsically, actively, misleading.


cheers
Sally

Sally Stowe
Centre for Visual Sciences, ANU



On Sat, February 7, 2009 2:47 am, colijn.1-at-osu.edu wrote:
}

}
}
} -------------------------------------------------------------------------
} ---
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
}
} Hi all,
}
}
} While brightness, contrast and gamma are fine to adjust. I would not
} feel comfortable in modifying the image to "cover up" a blemish. Assuming
} you are using a rubber stamp tool or something similar, you are modifying
} the information in the original image. You are then changing the
} information content of the picture. In my (not so humble) opinion, this
} is a dangerous step to take.
}
} Cheers,
} Henk
}
}
}
}
} At 08:52 AM 02/06/09, you wrote:
}
}
}
} } {...snip...}
} } I don't think any reasonable person would chastise somebody's
} } photographic evidence or scientific documentation because the contrast
} } was altered or an errant blemish was covered up in the name of producing
} } an eye-pleasing presentation.
} }
} } Stu Smalinskas, P.E.
} } Metallurgist
} } Plymouth, Michigan
} } SKF USA
} }
}
} {...snip...}
}
}
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all
} at once. Lately it doesn't seem to be working.
}
}
} ==============================Original
} Headers==============================
} 12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009
} 12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU
} (er6s1.ecr6.ohio-state.edu [164.107.76.2])
} 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n16FlnoU009705 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009
} 09:47:49 -0600
} 12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
} 12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
} 12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for
} Microscopy-at-microscopy.com;
} 12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST)
} 12, 26 -- Received: from HOC1.ecr6.ohio-state.edu
} 12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
} er6s1.ecr6.ohio-state.edu 12, 26 -- (PMDF V6.3-x18 #31556)
} 12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri,
} 12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST)
} 12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500
} 12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
} 12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and
} Validity
} 12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
} 12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
} 12, 26 -- To: smalinskas-at-yahoo.com
} 12, 26 -- Cc: Microscopy-at-microscopy.com
} 12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu}
} 12, 26 -- MIME-version: 1.0
} 12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
} 12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
} 12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
} ==============================End of -
} Headers==============================
}
}



==============================Original Headers==============================
13, 25 -- From sally.stowe-at-anu.edu.au Fri Feb 6 16:42:26 2009
13, 25 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70])
13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16MgPSV007505
13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:42:26 -0600
13, 25 -- Received: from mail.rsbs.anu.edu.au (localhost.localdomain [127.0.0.1])
13, 25 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP id 1F1C935C20C
13, 25 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 09:42:16 +1100 (EST)
13, 25 -- Received: from 202.45.99.119
13, 25 -- (SquirrelMail authenticated user a274988)
13, 25 -- by mail.rsbs.anu.edu.au with HTTP;
13, 25 -- Sat, 7 Feb 2009 09:42:16 +1100 (EST)
13, 25 -- Message-ID: {3050.202.45.99.119.1233960136.squirrel-at-mail.rsbs.anu.edu.au}
13, 25 -- In-Reply-To: {200902061547.n16FlvsM009979-at-ns.microscopy.com}
13, 25 -- References: {200902061547.n16FlvsM009979-at-ns.microscopy.com}
13, 25 -- Date: Sat, 7 Feb 2009 09:42:16 +1100 (EST)
13, 25 -- Subject: Re: Image Processing -- Ethics and Validity
13, 25 -- From: Sally.Stowe-at-anu.edu.au
13, 25 -- To: microscopy-at-microscopy.com
13, 25 -- User-Agent: SquirrelMail/1.5.1
13, 25 -- MIME-Version: 1.0
13, 25 -- Content-Type: text/plain;charset=iso-8859-1
13, 25 -- Content-Transfer-Encoding: 8bit
13, 25 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information
13, 25 -- X-RSBS-MailScanner: Found to be clean
13, 25 -- X-MailScanner-From: sally.stowe-at-anu.edu.au
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 6 Feb 2009 17:41:07 -0600
Subject: [Microscopy] Re: Carbon Rods versus Braids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Interesting discussion. I remember, quite well, the dark/black carbon
rods from Union Carbide. In fact, I checked out our stock and we have
several of the dark/black rods, which are softer and have the
appearence of being "dusted" with a black coating. Maybe they are
more crumbly and this represents surface material being shed. The
majority of our stock, UltraCarbon, is very hard, silver/gray in
appearance and has a slick surface. I know these require a lot of
current to evaporate.

Have you evaluated the braided carbon strands for use in your
application? We have been using them for some time now to produce
carbon coatings to support plastic substrates, to strengthen sections
and for conductivity coatings in X-ray analytical studies.They seem
to be OK but I have not compared the quality of the coatings to the
dark/black carbon. Something to add to my to-do list.

JB

} I too, am looking for a new supply of 1/8"
} carbon rods that don't require such high
} currents, and therefore produce such high heat
} loads, compared to the "older" ones that I have
} left. These older ones only need about 16-18
} amps to evaporate from a 3mm long x 0.8mm
} diameter tip that I sharpen on it. These
} low-current rods are a very dark black, and I
} think a bit softer than the lighter gray silvery
} ones that I also have and which take the high
} currents to evaporate. This later type seems to
} be all that's available out there now.
}
} In fact, I contacted John at Ladd about a year
} ago and he told me those black ones, which were
} originally purchased from Ladd years ago (Ladd
} part # 30267), are no longer available. I would
} ask him again now what kind of carbon rods is he
} talking about that only take 18-20 amps as he
} mentioned?
}
} I have a small stash of the black ones left, so
} I hope to find some more before I run out. I did
} try to contact the McMaster-Carr company about
} their carbon rods. The smallest ones they have
} are too big in diameter for our EM applications,
} but I never got a response back from them.
}


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Fri Feb 6 17:41:06 2009
8, 19 -- Received: from cstmta4.siu.edu (cstmta4.siu.edu [131.230.1.4])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Nf65I025153
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 17:41:06 -0600
8, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
8, 19 -- by cstmta4.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n16Nf5bi011455
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 17:41:06 -0600 (CST)
8, 19 -- Mime-Version: 1.0
8, 19 -- Message-Id: {a06240805c5b275c30868-at-[131.230.177.136]}
8, 19 -- In-Reply-To: {200902062238.n16Mc47f029587-at-ns.microscopy.com}
8, 19 -- References: {200902062238.n16Mc47f029587-at-ns.microscopy.com}
8, 19 -- Date: Fri, 6 Feb 2009 17:41:03 -0600
8, 19 -- To: Microscopy-at-microscopy.com
8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 19 -- Subject: Re: [Microscopy] Carbon Rods versus Braids
8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 19 -- X-Spam-Score: 0.00%
8, 19 -- X-MASF: 0.00%
8, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Fri, 6 Feb 2009 17:54:35 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hans;
What is the frequency of the field? 10mGauss should strike fear in your heart. Around trains, the high field can accompany the arrival of the train, and the effect it has on the image is that the whole microscope goes out of alignment. Best to look for a different building! If you get bludgeoned by management, try a Spicer field cancellation system:

http://www.spicerconsulting.com/index.htm

they are represented in the Netherlands by

Elektronen-Optik-Service GmbH
Zum Lonnenhohl 46
44319 Dortmund
GERMANY

Tel: +49 (0)231 722 11 22

http://www.eos-do.de/

I know that our FEI FIBs have Spicer systems built in, so FEI may also be able to supply a system. Remember that these systems do not eliminate varying magnetic fields; they attenuate them. They are also frequency dependent.

John Mardinly,
Numonyx


-----Original Message-----
X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl]
Sent: Thursday, February 05, 2009 8:19 AM
To: MARDINLY, A

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.janssen-at-nki.nl
Name: Hans Janssen

Organization: Netherlands Cancer Institute

Title-Subject: [Filtered] How to get rid of a magnetic field

Question: We want to move our EMís to a building
close to tram tracks. At pre-installation
measurements by FEI a distorting field of 10
mGauss (4.4 mGauss is the limit for a Tecnai12)
was found. This building was a former MRI
location; in the walls are steel (2cm thick)
plating and a copper Faraday cage both with large
holes in the ceiling. Can you give me advice on
preventing this magnetic field? Is restoring the
Faraday cage and/or the steel cage enough? Or do
we need a mu-ferro or a Helmholtz cage? What will
be the approximate costs of these?

Best regards, Hans


Login Host: 194.171.7.39
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009
9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832
9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600
9, 13 -- Mime-Version: 1.0
9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]}
9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600
9, 13 -- To: microscopy-at-microscopy.com
9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver)
9, 13 -- Subject: viaWWW: How to get rid of a magnetic field
9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 13 -- Content-Transfer-Encoding: 8bit
9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832
==============================End of - Headers==============================



==============================Original Headers==============================
24, 29 -- From A.MARDINLY-at-numonyx.com Fri Feb 6 17:54:34 2009
24, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16NsYBM006872
24, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Feb 2009 17:54:34 -0600
24, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
24, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id BEFE21E8422;
24, 29 -- Fri, 6 Feb 2009 17:03:53 -0500 (EST)
24, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
24, 29 -- Fri, 6 Feb 2009 18:54:33 -0500
24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 29 -- Content-class: urn:content-classes:message
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Type: text/plain;
24, 29 -- charset="iso-8859-1"
24, 29 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field
24, 29 -- Date: Fri, 6 Feb 2009 18:53:37 -0500
24, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9DD9D3A-at-EXDRESBENMX012.numonyx.local}
24, 29 -- In-Reply-To: {200902051618.n15GIrfQ003072-at-ns.microscopy.com}
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- Thread-Topic: [Microscopy] viaWWW: How to get rid of a magnetic field
24, 29 -- Thread-Index: AcmHrZuX9Xe3jDGQQTSzo2BWfJRoQwBB0zAQ
24, 29 -- References: {200902051618.n15GIrfQ003072-at-ns.microscopy.com}
24, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
24, 29 -- To: {j.janssen-at-nki.nl}
24, 29 -- Cc: {Microscopy-at-Microscopy.com}
24, 29 -- X-OriginalArrivalTime: 06 Feb 2009 23:54:33.0786 (UTC) FILETIME=[427FB1A0:01C988B6]
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16NsYBM006872
==============================End of - Headers==============================




From: pwje-at-sympatico.ca
Date: Fri, 6 Feb 2009 20:02:34 -0600
Subject: [Microscopy] Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello to all,
I have been through the Carbon Rod problems with an old Denton which only
had a 10 Amp power supply.
We ended up changing the power supply for a 20 Amp unit and using Carbon
Rods from

Carbone of America
900 Harrison Street
Bay City, MI 48708

Ask for SPK type carbon rods they are a little softer than some of the other
types of compound rod.

Attention Jamie 989 894 2911
I found her to be very helpful and knowledgeable in solving this problem.
My customer hasn't called me back complaining about poor coating or
sputtering since the changes.

Good luck
Peter Earl
Electrovac Technologies


==============================Original Headers==============================
6, 27 -- From pwje-at-sympatico.ca Fri Feb 6 20:02:34 2009
6, 27 -- Received: from blu0-omc3-s6.blu0.hotmail.com (blu0-omc3-s6.blu0.hotmail.com [65.55.116.81])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1722XMu025315
6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 20:02:34 -0600
6, 27 -- Received: from BLU0-SMTP75 ([65.55.116.74]) by blu0-omc3-s6.blu0.hotmail.com with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Fri, 6 Feb 2009 18:02:33 -0800
6, 27 -- X-Originating-IP: [76.65.17.200]
6, 27 -- X-Originating-Email: [pwje-at-sympatico.ca]
6, 27 -- Message-ID: {BLU0-SMTP75CEE35B43BEAF798EDFC6B9BE0-at-phx.gbl}
6, 27 -- Received: from peterf0x4h0jpn ([76.65.17.200]) by BLU0-SMTP75.blu0.hotmail.com over TLS secured channel with Microsoft SMTPSVC(6.0.3790.2668);
6, 27 -- Fri, 6 Feb 2009 18:02:32 -0800
6, 27 -- From: "Peter Earl" {pwje-at-sympatico.ca}
6, 27 -- To: {microscopy-at-microscopy.com}
6, 27 -- Subject: Carbon Rods
6, 27 -- Date: Fri, 6 Feb 2009 21:02:32 -0500
6, 27 -- MIME-Version: 1.0
6, 27 -- Content-Type: text/plain;
6, 27 -- format=flowed;
6, 27 -- charset="Windows-1252";
6, 27 -- reply-type=original
6, 27 -- Content-Transfer-Encoding: 7bit
6, 27 -- X-Priority: 3
6, 27 -- X-MSMail-Priority: Normal
6, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
6, 27 -- Disposition-Notification-To: "Peter Earl" {pwje-at-sympatico.ca}
6, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
6, 27 -- X-OriginalArrivalTime: 07 Feb 2009 02:02:32.0538 (UTC) FILETIME=[236443A0:01C988C8]
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Sat, 7 Feb 2009 07:02:28 -0600
Subject: [Microscopy] Re: Image Processing -- Ethics and Validity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My very humble opinion:

There exist some software packages that document every manipulation ever
done on an image. In some contexts this might be useful for a lab or
organization to implement. This is possible to do this with notebooks as
well. In spite of any such systems I think it will always be possible
for a worker to be intentionally deceptive with digital images like any
other media.

Brightness, contrast and gamma have been listed as legitimate
adjustments. Rossner and Yamada (The Journal of Cell Biology,
Volume 166, Number 1, July 5, 2004 11–15;
http://www.jcb.org/cgi/doi/10.1083/jcb.200406019 (an excellent reference
paper for all persons starting with imaging work)) list gamma as
altering the image in a way that requires disclosure. In the film and
paper era we altered gamma freely, since films almost absolutely had
gamma curves not equal to zero and by choice of paper you could
compensate this characteristic to your taste and most people could tell
if the print looked normal, or hard - with lost tones, but this was all
considered fair play. Worse was the reciprocity failure of silver-based
films, especially color ones, at exposures that might be used for
fluorescence photos - at exposures greater than one second and worse for
longer exposures. With reciprocity failure, 2 objects differing only
slightly in brightness, would be greatly different in brightness in the
captured image, thus automatically cleaning up slightly dimmer backgrounds.

The human eye has the capacity for tremendous dynamic range so it sees
into the shadows. Most media used today to present images do not have
more than 8-bit range, and monitors where people work with the images
have no more typically. So a sample/specimen with details in the dark
areas that the eye can adjust to separate, if recorded with even a
12-bit camera, the final image presented on the monitor or printed will
have to be adjusted to lose some shades, or have the gamma adjusted to
present brightnesses in a non-linear way to separate the darker areas.

At the bright end, the human eye is not so good and saturates at high
intensities and can't separate close values well, where a CCD properly
exposed can capture these values linearly and they can be displayed
unaltered in contrast or gamma and make close, bright values discernable
in a way that the eye has difficulty to perceive. So here it is not
unethical to present what we CAN'T directly/easily observe.

Most people using a digital camera should know that almost no chip has a
perfect pixel array, and most have maps stored to "correct defective
pixels", and this may or may not be obvious or accessible to the user.
This operation absolutely fabricates data to fill in the bad pixels. The
Diagnostic Imaging Spot cameras I have used have a checkbox so that
this can be disabled by choice; most people want a nice picture and
leave it checked. Most people know not to make the essence of a
publication about the value of one pixel.

What I tell people working at our facilities is that the captured and
presented image should substantially represent what another researcher
would see if they were to observe the same slide through the microscope;
anything to change this simple rule should be explained in the
presentation of the micrograph. If this is the case there will never be
any error of falsification.

It really does, in the end, come down to the integrity of the
researcher. Original raw data always needs to be saved. Manipulations
need to be documented so the operations could be repeated exactly, if
challenged, and the methods to create any image that would not be
directly observable by another should disclosed to the public as
processing since it does contribute to what is presented as "data".

Dale

colijn.1-at-osu.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi all,
}
} While brightness, contrast and gamma are fine to adjust. I would not
} feel comfortable in modifying the image to "cover up" a
} blemish. Assuming you are using a rubber stamp tool or something
} similar, you are modifying the information in the original
} image. You are then changing the information content of the
} picture. In my (not so humble) opinion, this is a dangerous step to take.
}
} Cheers,
} Henk
}
}
}
} At 08:52 AM 02/06/09, you wrote:
}
}
} } {...snip...}
} } I don't think any reasonable person would chastise somebody's
} } photographic evidence or scientific documentation because the
} } contrast was altered or an errant blemish was covered up in the name
} } of producing an eye-pleasing presentation.
} }
} } Stu Smalinskas, P.E.
} } Metallurgist
} } Plymouth, Michigan
} } SKF USA
}
} {...snip...}
}
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all
} at once. Lately it doesn't seem to be working.
}
}
} ==============================Original Headers==============================
} 12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009
} 12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
} 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FlnoU009705
} 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:47:49 -0600
} 12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
} 12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
} 12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
} 12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST)
} 12, 26 -- Received: from HOC1.ecr6.ohio-state.edu
} 12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
} 12, 26 -- (PMDF V6.3-x18 #31556)
} 12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri,
} 12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST)
} 12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500
} 12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
} 12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and Validity
} 12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
} 12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
} 12, 26 -- To: smalinskas-at-yahoo.com
} 12, 26 -- Cc: Microscopy-at-microscopy.com
} 12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu}
} 12, 26 -- MIME-version: 1.0
} 12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
} 12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
} 12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com}
} ==============================End of - Headers==============================

==============================Original Headers==============================
10, 20 -- From dac-at-research.umass.edu Sat Feb 7 07:02:28 2009
10, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n17D2QWe032390
10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 07:02:27 -0600
10, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged))
10, 20 -- (authenticated bits=0)
10, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n17D2NlT020950
10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 08:02:24 -0500
10, 20 -- Message-ID: {498D86A2.90006-at-research.umass.edu}
10, 20 -- Date: Sat, 07 Feb 2009 08:03:30 -0500
10, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
10, 20 -- MIME-Version: 1.0
10, 20 -- To: Microscopy List {microscopy-at-microscopy.com}
10, 20 -- Subject: Re: [Microscopy] Image Processing -- Ethics and Validity
10, 20 -- References: {200902061551.n16FpwSc021122-at-ns.microscopy.com}
10, 20 -- In-Reply-To: {200902061551.n16FpwSc021122-at-ns.microscopy.com}
10, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Sat, 7 Feb 2009 15:34:11 -0600
Subject: [Microscopy] MSA Policy on Digital Image Processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Along this thread many years ago the Microscopy Society of America
issued a position on image manipulation processing and storage.
It is documented here:

http://microscopy.org/MSAUnits/Education/ImagePolicy.html

the text of which is reproduced below.

"Ethical digital imaging requires that the original uncompressed
image file be stored on archival media (e.g., CD-R) without any image
manipulation or processing operation. All parameters of the
production and acquisition of this file, as well as any subsequent
processing steps, must be documented and reported to ensure
reproducibility.

Generally, acceptable (non-reportable) imaging operations include
gamma correction, histogram stretching, and brightness and contrast
adjustments. All other operations (such as Unsharp-Masking, Gaussian
Blur, etc.) must be directly identified by the author as part of the
experimental methodology. However, for diffraction data or any other
image data that is used for subsequent quantification, all imaging
operations must be reported."

This policy was formulated by the Digital Image Processing & Ethics
Group of the MSA Education Committee and was adopted as MSA policy at
the Summer Council meeting August 2-3, 2003.


Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Sat Feb 7 15:34:10 2009
9, 11 -- Received: from [10.5.0.80] (msdvpn8.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n17LY2dO001998
9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 15:34:08 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p0624080ac5b3adc1d3a8-at-[10.5.0.80]}
9, 11 -- Date: Sun, 8 Feb 2009 08:03:59 +1030
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
9, 11 -- Subject: MSA Policy on Digital Image Processing
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rhsia-at-umaryland.edu
Date: Sun, 8 Feb 2009 15:01:03 -0600
Subject: [Microscopy] viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rhsia-at-umaryland.edu
Name: Ru-ching Hsia

Organization: U Maryland

Title-Subject: [Filtered] Core Facility data/sample storage

Question: Dear all,
I have been the director of a new EM Core
facility for just more than a year now. Besides
dealing with the wide variety of samples and EM
projects brought into our facility, I found
myself confronted with a huge amount of image
data, specimen blocks and grids, much more than
I am used to. After keeping all the blocks and
grids of each project for a year, we are running
out of grid boxes and have not figured out a best
storage and tracking system for grids. I know
there are many EM facility directors regularly
contribute to the List, I would like to find out
what are the policies of other EM Core Facilities
concerning keeping user/clientís embedded blocks,
grids and image data?
Do you keep all the blocks and grids after each
project or do you give everything back to your
clients after the completion of the project?
If you keep the grids and blocks, how long do you
keep them? What is the best way to store and
index the grids from different projects? We find
it easier to keep all the grids from one project
in one grid box but that means we need more than
50 grid boxes a year.
How about the images? Do you keep them all? Is
there any particular software that you use to
catalogue all the image files?
Before I sign off, I would like to thank everyone
in the list who generously shares his/her
invaluable knowledge here. I was not trained as
an electron microscopist, but was landed this job
by a total twist of fate. The list and the
archive have been a great resource for me. Thank
you all.
Sincerely,
Ru-ching


Login Host: 173.64.120.125
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009
8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov [130.202.238.72])
8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n18L0v6e017516
8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00 -0600
8, 13 -- Mime-Version: 1.0
8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]}
8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: Core Facility data/sample storage
8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 13 -- Content-Transfer-Encoding: 8bit
8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n18L0v6e017516
==============================End of - Headers==============================




From: kamlennon-at-yahoo.com
Date: Sun, 8 Feb 2009 16:44:09 -0600
Subject: [Microscopy] teaching: stopping points for preparing tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

I'm wading through my first semester of teaching EM to undergrads and am in need of some advice regarding preparing animal tissue for TEM. Given the set-up of my class (that was not designed by me, mind you), we are forced sometimes to stop part-way through a protocol and store the tissue for a day or so. In my experience with plant tissue, I've done this at various steps, however, I need your advice on stopping points for animal tissue. Any advice on when tissue can be stored (and when it cannot), would be much appreciated. For reference, we are doing a "standard" primary fixation in glut/phosphate buffer, secondary in OsO4/phosphate buffer, dehydration in either EtOH or acetone and embedding in Spurr's.

Thanks in advance for sharing your wisdom,
Kristen Lennon

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
202 Compton Science Center
Frostburg State University
101 Braddock Road
Frostburg, MD 21532

301-687-4697
k.lennon-at-frostburg.edu





==============================Original Headers==============================
7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009
7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n18Mi9uL003740
7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09 -0600
7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009 22:44:08 -0000
7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 20 -- s=s1024; d=yahoo.com;
7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP+uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe+WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C+TdSbza5Vd8gWep0=;
7, 20 -- X-YMail-OSG: Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi337dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC_z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6SRWqAFwIcHyUrRUQ--
7, 20 -- Received: from [96.239.128.209] by web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST
7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST)
7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
7, 20 -- Reply-To: kamlennon-at-yahoo.com
7, 20 -- Subject: teaching: stopping points for preparing tissue?
7, 20 -- To: microscopy-at-microscopy.com
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain; charset=us-ascii
7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com}
==============================End of - Headers==============================




From: Elliott-at-arizona.edu
Date: Sun, 8 Feb 2009 18:05:34 -0600
Subject: [Microscopy] Re: teaching: stopping points for preparing tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had best luck stopping at 70% EtOH. It is the only stopping
point where I don't see problems. That said, my samples are not your
samples.....
David


On Feb 8, 2009, at 3:47 PM, kamlennon-at-yahoo.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Hi Listers,
}
} I'm wading through my first semester of teaching EM to undergrads
} and am in need of some advice regarding preparing animal tissue for
} TEM. Given the set-up of my class (that was not designed by me,
} mind you), we are forced sometimes to stop part-way through a
} protocol and store the tissue for a day or so. In my experience
} with plant tissue, I've done this at various steps, however, I need
} your advice on stopping points for animal tissue. Any advice on
} when tissue can be stored (and when it cannot), would be much
} appreciated. For reference, we are doing a "standard" primary
} fixation in glut/phosphate buffer, secondary in OsO4/phosphate
} buffer, dehydration in either EtOH or acetone and embedding in
} Spurr's.
}
} Thanks in advance for sharing your wisdom,
} Kristen Lennon
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} 301-687-4697
} k.lennon-at-frostburg.edu
}
}
}
}
}
} ==============================Original
} Headers==============================
} 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009
} 7, 20 -- Received: from web84004.mail.mud.yahoo.com
} (web84004.mail.mud.yahoo.com [68.142.206.174])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} id n18Mi9uL003740
} 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09
} -0600
} 7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009
} 22:44:08 -0000
} 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 7, 20 -- s=s1024; d=yahoo.com;
} 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-
} To:Subject:To:MIME-Version:Content-Type:Message-ID;
} 7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP
} +uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe
} +WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C
} +TdSbza5Vd8gWep0=;
} 7, 20 -- X-YMail-OSG:
} Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi33
} 7dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC
} _z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6
} SRWqAFwIcHyUrRUQ--
} 7, 20 -- Received: from [96.239.128.209] by
} web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST
} 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
} 7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST)
} 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 7, 20 -- Reply-To: kamlennon-at-yahoo.com
} 7, 20 -- Subject: teaching: stopping points for preparing tissue?
} 7, 20 -- To: microscopy-at-microscopy.com
} 7, 20 -- MIME-Version: 1.0
} 7, 20 -- Content-Type: text/plain; charset=us-ascii
} 7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com}
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
5, 22 -- From Elliott-at-arizona.edu Sun Feb 8 18:05:33 2009
5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1905X2x019149
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 18:05:33 -0600
5, 22 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238])
5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 18A819B97D0
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:33 -0700 (MST)
5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126])
5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7513E9B97B9
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:32 -0700 (MST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1)
5, 22 -- In-Reply-To: {200902082247.n18MlA4R008086-at-ns.microscopy.com}
5, 22 -- References: {200902082247.n18MlA4R008086-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
5, 22 -- Message-Id: {A8761809-081C-4B60-A8A9-24CFE82D3397-at-arizona.edu}
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- From: David Elliott {Elliott-at-arizona.edu}
5, 22 -- Subject: Re: [Microscopy] teaching: stopping points for preparing tissue?
5, 22 -- Date: Sun, 8 Feb 2009 17:05:30 -0700
5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com}
5, 22 -- X-Mailer: Apple Mail (2.753.1)
5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Sun, 8 Feb 2009 23:12:42 -0600
Subject: [Microscopy] Current Biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Andrea,

Our SEM studies of hair are purely morphological.
We look for things like twists, kinks, knots, longitudinal impressions, or
even invaginations of the hair shaft (so-called "bamboo hair").
We also look at the cuticle for signs of loss or fracture.
Hair shaft abnormalities may be associated with skin disorders or inborn
errors of metabolism. Bear in mind that exogenous factors can also affect
hair morphology.

We also look at hair using polarised light which may produce lovely banding
patterns, eg, the disease called trichothiodystrophy produces alternating
light and dark bands under polarised light (so-called "tiger-tail" pattern).
It's thought to be the result of low sulphur content within the hair.

Regards,

John

Hi John,

I am curious about the baby hair you look at. Are you looking for
morphological abnormalities, elemental composition, or both? I have never
heard of this before.

Thanks,
Andrea

Andrea Blake Brothers
Senior Scientist, MMCC
Microscopy & Microanalysis
Characterization Center
andrea.brothers-at-biovail.com

(703) 480-5879 office
(703) 480-5943 fax

BIOVAIL
3701 Concorde Parkway
Chantilly, VA 20151

The information contained in this e-mail message may be privileged and
confidential information and is intended only for the use of the individual
and/or entity identified in the address of this message. If the reader of
this message is not the intended recipient, or an employee or agent
responsible to deliver it to the intended recipient, you are hereby
requested not to distribute or copy this communication. If you have received
this communication in error, please notify us immediately by calling us
collect at (703) 480-6000, or by so advising us by return e-mail. In this
circumstance, we request that you delete the original message from your
system.


-----Original Message-----
X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au]
Sent: Thursday, February 05, 2009 6:01 PM
To: Andrea Brothers

Hi Jon,

We have an old Hitachi S-520 SEM still operational.
Once or twice a year we are asked to look at paediatric hair specimens for
defects. Of course, babies can't tell what's wrong with them so looking at
hair samples can sometimes give you a clue as to what is the problem.
Ie, the hair defect may be one part of a wider syndrome.

Regards,

John Brealey

Senior Medical Scientist, Electron Microscopy Unit

T 08 8222 6612
F 08 8222 6425

www.sapathology.sa.gov.au

SA Pathology (TQEH)
Quality Pathology supporting Training and Research


==============================Original Headers==============================
29, 27 -- From john.brealey-at-imvs.sa.gov.au Sun Feb 8 23:12:42 2009
29, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
29, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n195CdcQ013965
29, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 23:12:41 -0600
29, 27 -- X-IronPort-AV: E=Sophos;i="4.37,403,1231075800";
29, 27 -- d="scan'208";a="9491193"
29, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
29, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 09 Feb 2009 15:42:36 +1030
29, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
29, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
29, 27 -- 8.1.263.0; Mon, 9 Feb 2009 15:41:45 +1030
29, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au
29, 27 -- (Postfix) with ESMTP id 6FE2734BA4 for {Microscopy-at-microscopy.com} ; Mon, 9
29, 27 -- Feb 2009 15:42:36 +1030 (CST)
29, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
29, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
29, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
29, 27 -- Subject: SEM of Hair
29, 27 -- Date: Mon, 9 Feb 2009 15:42:35 +1030
29, 27 -- Organization: IMVS
29, 27 -- Message-ID: {000001c98a75$05166410$5954150a-at-41347i}
29, 27 -- MIME-Version: 1.0
29, 27 -- Content-Type: text/plain; charset="us-ascii"
29, 27 -- Content-Transfer-Encoding: 7bit
29, 27 -- X-Mailer: Microsoft Office Outlook 11
29, 27 -- Thread-Index: AcmKdQTt+EepJkF0Qx23XNV1d8kjjw==
29, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Mon, 9 Feb 2009 00:50:31 -0600
Subject: [Microscopy] Fwd: teaching: stopping points for preparing tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The point after GA fixation and 2-3 rinses is a good early point.
After OsO4 and rinses is not bad too. Lower concentration alcohols is
a big NO. 70% is OK to leave overnight in the fridge and convenient to
combine with UA staining, making it 1.5% UA in 70% ethanol.

In general, any excessive time in alcohol or acetone causes
extraction, so samples should not be left there for days. Same for
diluted epoxy. Acetone has been reported to be less extractive during
dehydration than ethanol, but it is harder to handle.

There is one more point worth mentioning. I've never seen it described
anywhere, but once had to fix an important sample on the eve of
vacation. I reasoned that undiluted ethanol in the freezer (-20C)
should minimize any extraction and will also not protect from freezing
damage. A week later (yes, US vacations...) I embedded the samples,
and all was perfect.

Vlad

________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

} Hi Listers,
}
} I'm wading through my first semester of teaching EM to undergrads
} and am in need of some advice regarding preparing animal tissue for
} TEM. Given the set-up of my class (that was not designed by me, mind
} you), we are forced sometimes to stop part-way through a protocol
} and store the tissue for a day or so. In my experience with plant
} tissue, I've done this at various steps, however, I need your advice
} on stopping points for animal tissue. Any advice on when tissue can
} be stored (and when it cannot), would be much appreciated. For
} reference, we are doing a "standard" primary fixation in glut/
} phosphate buffer, secondary in OsO4/phosphate buffer, dehydration in
} either EtOH or acetone and embedding in Spurr's.
}
} Thanks in advance for sharing your wisdom,
} Kristen Lennon
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} 202 Compton Science Center
} Frostburg State University
} 101 Braddock Road
} Frostburg, MD 21532
}
} 301-687-4697
} k.lennon-at-frostburg.edu
}
}
}
}
}
} ==============================Original
} Headers==============================
} 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009
} 7, 20 -- Received: from web84004.mail.mud.yahoo.com
} (web84004.mail.mud.yahoo.com [68.142.206.174])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with



From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 30 Jan 2009 11:06:57 -0600
Subject: [Microscopy] SEM holder for TEM grids : Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to all which respond to my inquiry.

If there are no wonders, there are nevertheless some interesting (and
simple) idees to follow up.

Jacques


-------- Message original --------
Sujet: [Microscopy] SEM holder for TEM grids

Hi all

As there is again some demand here for observations of TEM grids by SEM,
I took out of the drawer the home made TEM grids holders I had machined
a while ago. And non of them is really practical to use. The first was
made with an EM300 tip, and works right, but it's a one shot and for
limited to the jeol 840 serie stage. The second is delicate to use, and
I fear to bent the grids each time I take them away from the holder.

So what kind of holder do other use for TEM grids (only SEM
observations, no STEM), which allows very short WD and an easy way to
mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues,
but certainly not at all sources. And users advices are very usfull !

I'm interested in any ideas and/or squetches for home manufacturing in
our workshop, and/or for documentions, users advices and manufacturer
doc and quoting etc.

Thanks in advance, and have a good WE !

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 30 11:03:10 2009
8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153])
8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0UH394k027846
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 11:03:10 -0600
8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
8, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n0UH38SA065122
8, 29 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 18:03:08 +0100 (CET)
8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
8, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 29 -- (No client certificate requested)
8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 766C43EC02E
8, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 17:52:58 +0100 (CET)
8, 29 -- Message-ID: {49833069.2050502-at-ipcms.u-strasbg.fr}
8, 29 -- Date: Fri, 30 Jan 2009 17:52:57 +0100
8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
8, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
8, 29 -- MIME-Version: 1.0
8, 29 -- To: Microscopy-at-microscopy.com
8, 29 -- Subject: SEM holder for TEM grids
8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 29 -- Content-Transfer-Encoding: 8bit
8, 29 -- X-IPCMS-MailScanner: Found to be clean
8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 30 Jan 2009 18:03:08 +0100 (CET)
8, 29 -- X-Virus-Scanned: ClamAV 0.94.2/8924/Fri Jan 30 16:10:48 2009 on mr3.u-strasbg.fr
8, 29 -- X-Virus-Status: Clean
8, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
8, 29 -- autolearn=disabled version=3.2.5
8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr3.u-strasbg.fr
==============================End of - Headers==============================


--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
21, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Feb 9 06:59:01 2009
21, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.158])
21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19Cwxhc021988
21, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 06:59:00 -0600
21, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
21, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n19CwtnQ055380
21, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:58:57 +0100 (CET)
21, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
21, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
21, 29 -- (No client certificate requested)
21, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 6B0B63EC002
21, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 13:58:46 +0100 (CET)
21, 29 -- Message-ID: {49902885.9090608-at-ipcms.u-strasbg.fr}
21, 29 -- Date: Mon, 09 Feb 2009 13:58:45 +0100
21, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
21, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
21, 29 -- MIME-Version: 1.0
21, 29 -- To: Microscopy-at-microscopy.com
21, 29 -- Subject: SEM holder for TEM grids : Thanks
21, 29 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed
21, 29 -- Content-Transfer-Encoding: 8bit
21, 29 -- X-IPCMS-MailScanner: Found to be clean
21, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
21, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.158]); Mon, 09 Feb 2009 13:58:57 +0100 (CET)
21, 29 -- X-Virus-Scanned: ClamAV 0.94.2/8967/Mon Feb 9 09:38:55 2009 on mr8.u-strasbg.fr
21, 29 -- X-Virus-Status: Clean
21, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
21, 29 -- autolearn=disabled version=3.2.5
21, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr8.u-strasbg.fr
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 9 Feb 2009 08:34:08 -0600
Subject: [Microscopy] viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ru-ching,

I would strongly recommend having your users take and store their own samples themselves, and the earlier you start having them do it, the better.

For years, we had been keeping samples in storage for our clients, until the volume became overwhelming. Samples, grids,blocks, etc. were piled everywhere. We are now in the very slow process of getting the samples back to those researchers who still want them. The problem is that in some cases, especially high-volume users, the resistance to taking their own samples to their own labs is, well, pretty intense. They got too used to having us do it for them, but then what happens is they might come in and want to review a project from years ago. If we can't find the exact set of specimens, blocks, or grids on short notice, it can get dicey.

We are considering instituting a storage fee to provide a little nudge to get people to take their own stuff back. Fees are very effective behavior modifiers and can be used not only for this, but for discouraging late-shows or no-shows on scopes, messes being left behind by sloppy users, failure to fill out forms and logbooks, etc. We generally find we only have to charge a behavior-mod fee (BMF) once or twice and that behavior has been modded.

Regarding images, we generally archive them here in digital form, which is not a huge problem with large, cheap hard-drives readily available. We used to back up images on CD's and DVD's, but now we generally leave on big hard drives backing up our primary hard drives on the scope computers. That said, we have very rarely been asked to dig up old images, so it may not really be necessary. It would not be unreasonable, in my opinion, to make the user responsible for this aspect of their work, too.

Every lab is different and we all operate under different sets of instructions, but this is how we go about things in our facility.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu]
Sent: Sunday, February 08, 2009 3:02 PM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rhsia-at-umaryland.edu
Name: Ru-ching Hsia

Organization: U Maryland

Title-Subject: [Filtered] Core Facility data/sample storage

Question: Dear all,
I have been the director of a new EM Core
facility for just more than a year now. Besides
dealing with the wide variety of samples and EM
projects brought into our facility, I found
myself confronted with a huge amount of image
data, specimen blocks and grids, much more than
I am used to. After keeping all the blocks and
grids of each project for a year, we are running
out of grid boxes and have not figured out a best
storage and tracking system for grids. I know
there are many EM facility directors regularly
contribute to the List, I would like to find out
what are the policies of other EM Core Facilities
concerning keeping user/clientís embedded blocks,
grids and image data?
Do you keep all the blocks and grids after each
project or do you give everything back to your
clients after the completion of the project?
If you keep the grids and blocks, how long do you
keep them? What is the best way to store and
index the grids from different projects? We find
it easier to keep all the grids from one project
in one grid box but that means we need more than
50 grid boxes a year.
How about the images? Do you keep them all? Is
there any particular software that you use to
catalogue all the image files?
Before I sign off, I would like to thank everyone
in the list who generously shares his/her
invaluable knowledge here. I was not trained as
an electron microscopist, but was landed this job
by a total twist of fate. The list and the
archive have been a great resource for me. Thank
you all.
Sincerely,
Ru-ching


Login Host: 173.64.120.125
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009
8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov [130.202.238.72])
8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n18L0v6e017516
8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00 -0600
8, 13 -- Mime-Version: 1.0
8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]}
8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: Core Facility data/sample storage
8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 13 -- Content-Transfer-Encoding: 8bit
8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n18L0v6e017516
==============================End of - Headers==============================


==============================Original Headers==============================
25, 30 -- From TindallR-at-missouri.edu Mon Feb 9 08:34:08 2009
25, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19EY7ef007304
25, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 08:34:07 -0600
25, 30 -- X-IronPort-Anti-Spam-Filtered: true
25, 30 -- X-IronPort-Anti-Spam-Result: ApoEAOnNj0nRauUp/2dsb2JhbADCKiMBAQEHhAWISoJjAQGBNQaFdA
25, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
25, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 09 Feb 2009 08:34:06 -0600
25, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
25, 30 -- Mon, 9 Feb 2009 08:34:06 -0600
25, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
25, 30 -- Content-class: urn:content-classes:message
25, 30 -- MIME-Version: 1.0
25, 30 -- Content-Type: text/plain;
25, 30 -- charset="iso-8859-1"
25, 30 -- Subject: RE: [Microscopy] viaWWW: Core Facility data/sample storage
25, 30 -- Date: Mon, 9 Feb 2009 08:34:05 -0600
25, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DA6-at-UM-XMAIL08.um.umsystem.edu}
25, 30 -- In-Reply-To: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
25, 30 -- X-MS-Has-Attach:
25, 30 -- X-MS-TNEF-Correlator:
25, 30 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample storage
25, 30 -- Thread-Index: AcmKMI0d/imrqTxSTNe2aPrAmz2cEQAkPpmw
25, 30 -- References: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
25, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
25, 30 -- To: {rhsia-at-umaryland.edu}
25, 30 -- Cc: {microscopy-at-microscopy.com}
25, 30 -- X-OriginalArrivalTime: 09 Feb 2009 14:34:06.0042 (UTC) FILETIME=[760A6BA0:01C98AC3]
25, 30 -- Content-Transfer-Encoding: 8bit
25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19EY7ef007304
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Mon, 9 Feb 2009 08:53:04 -0600
Subject: [Microscopy] Re: viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ru-ching-
I've run a core facility for 20 years and I have
almost every sample I've ever prepared...blocks
and grids. The exceptions have been when
investigators have left this institution and were
planning on continuing their work at their new
home. I have given them their materials and
noted the transfer in my records.
My record system is a little redundant because it
has, over the years, gone from paper only, to
Excel to our on-line information management
system (LIMS).
Briefly:
Each request that comes in is logged. Our log
numbers consist of the last 2-digits of the
current calendar year, followed by next number in
sequence eg: 09-003 is followed by 09-004, etc..
This number is entered on the protocol sheet that
is created for each request, as well as on a
"Log'in" sheet that is kept on a clip board.
Also noted are the date, requester's name, head
of lab, type of sample (TEM, SEM, paraffin, cryo,
etc). There are columns for checking off when
the work is completed and when it has been billed.
We prepare a protocol sheet for each request on
which we detail how the sample(s) has been
handled.
Here's the redundancy: the samples are logged
into our LIMS, and I still enter it into the
Excel sheet, because it is the easiest to search.
As far as storage: Now that everyone is going to
digital imaging I will have to come up with a new
system. For years, I have stored my old grid
boxes and the white slider "match boxes" in which
I store my blocks, in the 500-sheet boxes from
8x10 photographic paper. They work out to hold
about 3-4 years worth of grid boxes (of the style
I use) and about 2 years worth of slide boxes.
Those are stashed on the top shelves of the wall
cabinets in my lab. (since I am a bit "vertically
challenged" I don't keep anything I need
ready-access to that high up!).
So yes, I have hundreds of grid boxes sitting in
storage, with samples going back to my
predecessor (back to 1982). the boxes are
labelled by month and year. I can always find
old grids when they are requested.
I have always given the negatives (& in the old
days, prints) back to the investigator. I now
keep copies of the scanned negatives or digital
images on a hard drive in the lab. The
investigator gets them on CD.
If you'd like more details, just write.
Good luck,
Lee
} ---------------------------------------------------------------------------
}
} Email: rhsia-at-umaryland.edu
} Name: Ru-ching Hsia
}
} Organization: U Maryland
}
} Title-Subject: [Filtered] Core Facility data/sample storage
}
} Question: Dear all,
} I have been the director of a new EM Core
} facility for just more than a year now. Besides
} dealing with the wide variety of samples and EM
} projects brought into our facility, I found
} myself confronted with a huge amount of image
} data, specimen blocks and grids, much more than
} I am used to. After keeping all the blocks and
} grids of each project for a year, we are running
} out of grid boxes and have not figured out a best
} storage and tracking system for grids. I know
} there are many EM facility directors regularly
} contribute to the List, I would like to find out
} what are the policies of other EM Core Facilities
} concerning keeping user/clientís embedded blocks,
} grids and image data?
} Do you keep all the blocks and grids after each
} project or do you give everything back to your
} clients after the completion of the project?
} If you keep the grids and blocks, how long do you
} keep them? What is the best way to store and
} index the grids from different projects? We find
} it easier to keep all the grids from one project
} in one grid box but that means we need more than
} 50 grid boxes a year.
} How about the images? Do you keep them all? Is
} there any particular software that you use to
} catalogue all the image files?
} Before I sign off, I would like to thank everyone
} in the list who generously shares his/her
} invaluable knowledge here. I was not trained as
} an electron microscopist, but was landed this job
} by a total twist of fate. The list and the
} archive have been a great resource for me. Thank
} you all.
} Sincerely,
} Ru-ching

--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org


==============================Original Headers==============================
4, 26 -- From lcgould-at-med.cornell.edu Mon Feb 9 08:53:01 2009
4, 26 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19Er0wH021537
4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 08:53:01 -0600
4, 26 -- Received: from mpx3.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120])
4, 26 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n19Eqw5r020643;
4, 26 -- Mon, 9 Feb 2009 09:52:58 -0500 (EST)
4, 26 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
4, 26 -- by mpx3.med.cornell.edu
4, 26 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
4, 26 -- with ESMTPA id {0KET00GC5009PP40-at-mpx3.med.cornell.edu} ; Mon,
4, 26 -- 09 Feb 2009 09:52:58 -0500 (EST)
4, 26 -- Date: Mon, 09 Feb 2009 09:52:55 -0500
4, 26 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
4, 26 -- Subject: Re: [Microscopy] viaWWW: Core Facility data/sample storage
4, 26 -- In-reply-to: {200902082110.n18LA9s2031773-at-ns.microscopy.com}
4, 26 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
4, 26 -- To: rhsia-at-umaryland.edu, Microscopy Listserver {microscopy-at-microscopy.com}
4, 26 -- Message-id: {p06230902c5b5eb64b6e5-at-[140.251.48.23]}
4, 26 -- MIME-version: 1.0
4, 26 -- Content-type: text/plain; charset=iso-8859-1; format=flowed
4, 26 -- References: {200902082110.n18LA9s2031773-at-ns.microscopy.com}
4, 26 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.9.144026
4, 26 -- X-PerlMx-Spam: Gauge=, IIIIIII, Probability=7%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0'
4, 26 -- Content-Transfer-Encoding: 8bit
4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19Er0wH021537
==============================End of - Headers==============================




From: nicholls-at-uic.edu
Date: Mon, 9 Feb 2009 09:10:33 -0600
Subject: [Microscopy] viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ru-ching

I agree totally with Randy. Once the project has been completed we expect
the users to take and store their own specimens. We also expect all users
to archive and store their own data. We started, 11 years ago, by also
storing them in digital form before taking them off instrument computers,
but after a few years the number of CD-R disks was becoming impossible.
There were only a very few occasions that users asked for the archived
images so we no longer do it.

Regards

Alan

At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Interim Associate Director - RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu


==============================Original Headers==============================
10, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:10:33 2009
10, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19FAWmG003455
10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:33 -0600
10, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241])
10, 20 -- (authenticated bits=0)
10, 20 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id n19FAVIM026822
10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:32 -0600
10, 20 -- Message-Id: {6.2.1.2.2.20090209085934.021d0600-at-mail.uic.edu}
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
10, 20 -- Date: Mon, 09 Feb 2009 09:10:30 -0600
10, 20 -- To: Microscopy-at-microscopy.com
10, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
10, 20 -- Subject: Re: [Microscopy] RE: viaWWW: Core Facility data/sample storage
10, 20 -- In-Reply-To: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
10, 20 -- References: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
10, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 9 Feb 2009 09:32:15 -0600
Subject: [Microscopy] RE: Philips EM300 plate film: no image on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As John Bozzola mentioned, there can be many reasons for a blank
negative, but one very valuable tool in diagnosing the cause of imaging
problems on negatives is the scope data that the instrument itself
flashes onto the negative. This would be the mag marker, kV
information, magnification, or whatever your particular microscope
records on the film.

For example, if the entire negative is blank, including the scope
information, then either the scope is completely failing to record
anything, the film was loaded emulsion side down, your chemicals are
bad, or you accidentally put the film in fixer before the developer
(been there, done that).

If the scope information looks normal, but the sample image is missing
or really light, then the problem is with the recording of the sample in
the scope, not with the developing side of things. Your exposure time
may be too brief or the sample exposure isn't happening at all.

If both the scope data and sample image are very light, then the problem
is almost always exhausted chemicals, overly diluted developer, very
cold developer, or developing times that are too short. Sometimes this
can also indicate improper exposure settings for the microscope, but I
think the mag marker, etc., exposure is generally set at the factory and
checked by the service engineers during set-up. I have personally never
seen it go wrong. Another possible cause is way outdated film, but this
would normally be accompanied by an overall gray fog and lousy contrast.

Hope some of this is useful.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Friday, February 06, 2009 3:57 PM
To: Tindall, Randy D.

}
} I just changed the filament for Philips EM300 and saturated, the
} illumination looks good and I observe the samples. But the problem is
} when I develop the negatives, it looks empty. There was no image,
} Please let me know what is the problem and why the film is empty.
} Thanks for your help.

=============


==============================Original Headers==============================
18, 29 -- From TindallR-at-missouri.edu Mon Feb 9 09:32:15 2009
18, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19FWD3Q017928
18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:32:14 -0600
18, 29 -- X-IronPort-Anti-Spam-Filtered: true
18, 29 -- X-IronPort-Anti-Spam-Result: ApoEAIvaj0nRauUo/2dsb2JhbADCfgEJhBSISoJYC4E3Bg
18, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
18, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Feb 2009 09:32:10 -0600
18, 29 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
18, 29 -- Mon, 9 Feb 2009 09:32:10 -0600
18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
18, 29 -- Content-class: urn:content-classes:message
18, 29 -- MIME-Version: 1.0
18, 29 -- Content-Type: text/plain;
18, 29 -- charset="us-ascii"
18, 29 -- Subject: RE: [Microscopy] RE: Philips EM300 plate film: no image on negatives
18, 29 -- Date: Mon, 9 Feb 2009 09:32:10 -0600
18, 29 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DA8-at-UM-XMAIL08.um.umsystem.edu}
18, 29 -- In-Reply-To: {200902062156.n16LulTb014586-at-ns.microscopy.com}
18, 29 -- X-MS-Has-Attach:
18, 29 -- X-MS-TNEF-Correlator:
18, 29 -- Thread-Topic: [Microscopy] RE: Philips EM300 plate film: no image on negatives
18, 29 -- Thread-Index: AcmIpc9Jb7kqJsWoTCiTowXM0NJ9zACIwEgA
18, 29 -- References: {200902062156.n16LulTb014586-at-ns.microscopy.com}
18, 29 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
18, 29 -- To: {microscopy-at-microscopy.com}
18, 29 -- X-OriginalArrivalTime: 09 Feb 2009 15:32:10.0904 (UTC) FILETIME=[932E2980:01C98ACB]
18, 29 -- Content-Transfer-Encoding: 8bit
18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19FWD3Q017928
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Mon, 9 Feb 2009 09:33:52 -0600
Subject: [Microscopy] RE: Philips EM300 plate film: no image on negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, I would like to thank every one for your valuable suggestions. I
tried all of them and find the following results.

As I told, I Lift the large screen and without transporting a film
cassette I pressed the exposure button.

I did not see an image on the small screen and I did not even see light
beam also. So please let me know if any one know the solution for this
problem or should I need service engineer to do this.

Sincerely
Kabilan
UMDNJ-NJDS
Newark, NJ, USA


==============================Original Headers==============================
5, 26 -- From velliyka-at-umdnj.edu Mon Feb 9 09:33:52 2009
5, 26 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19FXojn019721
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:33:51 -0600
5, 26 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1])
5, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 7E07E4C087
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:33:46 -0500 (EST)
5, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
5, 26 -- by zix04.umdnj.edu (Proprietary) with ESMTP id BECD650C023
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:32:05 -0500 (EST)
5, 26 -- Received: from ([10.32.15.171])
5, 26 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.6716359;
5, 26 -- Mon, 09 Feb 2009 10:27:25 -0500
5, 26 -- MIME-version: 1.0
5, 26 -- Content-transfer-encoding: 7BIT
5, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
5, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
5, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
5, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KET00BY41LPNSA0-at-umduwc02.umdnj.edu} for
5, 26 -- Microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:27:25 -0500 (EST)
5, 26 -- Message-id: {1fac03542de90fc883d302d713f7ebe0-at-umdnj.edu}
5, 26 -- To: Microscopy-at-microscopy.com
5, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
5, 26 -- Subject: RE: Philips EM300 plate film: no image on negatives
5, 26 -- Date: Sun, 08 Feb 2009 22:30:38 -0500
5, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: nicholls-at-uic.edu
Date: Mon, 9 Feb 2009 09:41:23 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hans

You do not say if the problem is ac field or a dc field. The existing
Faraday cage would help attenuate constant dc fields and would also
significantly reduce high frequency ac fields but would probably not
significantly attenuate 50/60Hz fields.

The chances are that if it is due to the trams it is a varying dc field
that occurs as each tram enters the section and starts to draw power. Field
cancelation systems work best when the field to be cancelled does not vary,
that is it is always the same frequency with always the same amplitude and
shape - we have an IDE Helmholtz coil system that allowed our field
emission TEM/STEM to be used while facilities spent several months looking
for the source of a 40mG pure, constant 60Hz field which suddenly appeared
one winter! This system cost ~$40K ten years ago.

In a previous life I was involved with a dedicated STEM being installed in
the former East Germany. The lab there had tram tracks on one side of the
building with a power feeder for the trams running along another wall. In
this case the installation engineer used a Hall effect probe to detect the
dc field shift and fed the opposite offset into a set of alignment coils.
This worked and stopped the image annoyingly jumping side to side as the
trams entered the section. The cost in this case was a few dollars in parts
and a few hours of labor!

The instrument you want to move is a Tecnai12? If this is used for life
science work at relatively low magnifications ( {100K) then the effect of
the field may not be noticeable. The microscope would not meet performance
specifications but you would not be using it anywhere near that limit. This
would not be ideal, but may be cheaper!

In general the best approach to fields, wether dc or ac is to avoid them!

Good luck

Alan



}
} -----Original Message-----
} X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl]
} Sent: Thursday, February 05, 2009 8:19 AM
} Subject: [Microscopy] viaWWW: How to get rid of a magnetic field
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Interim Associate Director - RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu


==============================Original Headers==============================
15, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:41:23 2009
15, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184])
15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19FfM3I005185
15, 20 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:41:22 -0600
15, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241])
15, 20 -- (authenticated bits=0)
15, 20 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id n19FfLJM004640
15, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
15, 20 -- Mon, 9 Feb 2009 09:41:21 -0600
15, 20 -- Message-Id: {6.2.1.2.2.20090209091207.021d0370-at-mail.uic.edu}
15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
15, 20 -- Date: Mon, 09 Feb 2009 09:41:20 -0600
15, 20 -- To: j.janssen-at-nki.nl, microscopy-at-microscopy.com
15, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
15, 20 -- Subject: Re: [Microscopy] RE: viaWWW: How to get rid of a magnetic field
15, 20 -- In-Reply-To: {200902062355.n16NtCg3008051-at-ns.microscopy.com}
15, 20 -- References: {200902062355.n16NtCg3008051-at-ns.microscopy.com}
15, 20 -- Mime-Version: 1.0
15, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
15, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Mon, 9 Feb 2009 09:56:19 -0600
Subject: [Microscopy] teaching: stopping points for preparing tissue?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Buffer after glutaraldehyde or buffer after osmium are the best places
to stop and store tissue. Storage in any concentration of alcohol (or
acetone or prop.oxide) is NOT a good idea, cytoplasm will be extracted.
This is well documented in the literature. If you can get to pure resin
put the vials in the refrigerator overnight. Just remember to let things
warm to room temp. before opening to avoid condensation.

Geoff

Elliott-at-arizona.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I have had best luck stopping at 70% EtOH. It is the only stopping
} point where I don't see problems. That said, my samples are not your
} samples.....
} David
}
}
} On Feb 8, 2009, at 3:47 PM, kamlennon-at-yahoo.com wrote:
}
}
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/
} } MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } Hi Listers,
} }
} } I'm wading through my first semester of teaching EM to undergrads
} } and am in need of some advice regarding preparing animal tissue for
} } TEM. Given the set-up of my class (that was not designed by me,
} } mind you), we are forced sometimes to stop part-way through a
} } protocol and store the tissue for a day or so. In my experience
} } with plant tissue, I've done this at various steps, however, I need
} } your advice on stopping points for animal tissue. Any advice on
} } when tissue can be stored (and when it cannot), would be much
} } appreciated. For reference, we are doing a "standard" primary
} } fixation in glut/phosphate buffer, secondary in OsO4/phosphate
} } buffer, dehydration in either EtOH or acetone and embedding in
} } Spurr's.
} }
} } Thanks in advance for sharing your wisdom,
} } Kristen Lennon
} }
} } Kristen A. Lennon, Ph.D.
} } Lecturer, Department of Biology
} } 202 Compton Science Center
} } Frostburg State University
} } 101 Braddock Road
} } Frostburg, MD 21532
} }
} } 301-687-4697
} } k.lennon-at-frostburg.edu
} }
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009
} } 7, 20 -- Received: from web84004.mail.mud.yahoo.com
} } (web84004.mail.mud.yahoo.com [68.142.206.174])
} } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} } id n18Mi9uL003740
} } 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09
} } -0600
} } 7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009
} } 22:44:08 -0000
} } 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} } 7, 20 -- s=s1024; d=yahoo.com;
} } 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-
} } To:Subject:To:MIME-Version:Content-Type:Message-ID;
} } 7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP
} } +uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe
} } +WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C
} } +TdSbza5Vd8gWep0=;
} } 7, 20 -- X-YMail-OSG:
} } Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi33
} } 7dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC
} } _z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6
} } SRWqAFwIcHyUrRUQ--
} } 7, 20 -- Received: from [96.239.128.209] by
} } web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST
} } 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1
} } 7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST)
} } 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} } 7, 20 -- Reply-To: kamlennon-at-yahoo.com
} } 7, 20 -- Subject: teaching: stopping points for preparing tissue?
} } 7, 20 -- To: microscopy-at-microscopy.com
} } 7, 20 -- MIME-Version: 1.0
} } 7, 20 -- Content-Type: text/plain; charset=us-ascii
} } 7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com}
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original Headers==============================
} 5, 22 -- From Elliott-at-arizona.edu Sun Feb 8 18:05:33 2009
} 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169])
} 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1905X2x019149
} 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 18:05:33 -0600
} 5, 22 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238])
} 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 18A819B97D0
} 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:33 -0700 (MST)
} 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126])
} 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7513E9B97B9
} 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:32 -0700 (MST)
} 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1)
} 5, 22 -- In-Reply-To: {200902082247.n18MlA4R008086-at-ns.microscopy.com}
} 5, 22 -- References: {200902082247.n18MlA4R008086-at-ns.microscopy.com}
} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
} 5, 22 -- Message-Id: {A8761809-081C-4B60-A8A9-24CFE82D3397-at-arizona.edu}
} 5, 22 -- Content-Transfer-Encoding: 7bit
} 5, 22 -- From: David Elliott {Elliott-at-arizona.edu}
} 5, 22 -- Subject: Re: [Microscopy] teaching: stopping points for preparing tissue?
} 5, 22 -- Date: Sun, 8 Feb 2009 17:05:30 -0700
} 5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com}
} 5, 22 -- X-Mailer: Apple Mail (2.753.1)
} 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
} ==============================End of - Headers==============================
}
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
7, 28 -- From mcauliff-at-umdnj.edu Mon Feb 9 09:56:18 2009
7, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124])
7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19FuGMx027287
7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:56:17 -0600
7, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
7, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 8EDA2A7B5B
7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:56:15 -0500 (EST)
7, 28 -- Received: from umdnj.edu (unknown [10.32.15.102])
7, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 1FD61A7B6B
7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:53:55 -0500 (EST)
7, 28 -- Received: from ([10.32.15.171])
7, 28 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6442831;
7, 28 -- Mon, 09 Feb 2009 10:49:24 -0500
7, 28 -- MIME-version: 1.0
7, 28 -- Content-transfer-encoding: 7BIT
7, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
7, 28 -- Received: from [127.0.0.1] ([10.32.15.102])
7, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
7, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KET00BCN2MCNSD0-at-umduwc02.umdnj.edu} for
7, 28 -- microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:49:24 -0500 (EST)
7, 28 -- Message-id: {499050D4.2010900-at-umdnj.edu}
7, 28 -- Date: Mon, 09 Feb 2009 10:50:44 -0500
7, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
7, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
7, 28 -- To: Elliott-at-arizona.edu, microscopy-at-microscopy.com
7, 28 -- Subject: Re: [Microscopy] Re: teaching: stopping points for preparing tissue?
7, 28 -- References: {200902090006.n1906fhk020682-at-ns.microscopy.com}
7, 28 -- In-reply-to: {200902090006.n1906fhk020682-at-ns.microscopy.com}
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Mon, 9 Feb 2009 10:49:49 -0600
Subject: [Microscopy] viaWWW: How to get rid of a magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alan;
Did they find the source of the 40mG field? Can you reveal what it was and the why and how of how it suddenly appeared?

John Mardinly,
Numonyx


-----Original Message-----
X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu]
Sent: Monday, February 09, 2009 7:51 AM
To: MARDINLY, A

Hans

You do not say if the problem is ac field or a dc field. The existing
Faraday cage would help attenuate constant dc fields and would also
significantly reduce high frequency ac fields but would probably not
significantly attenuate 50/60Hz fields.

The chances are that if it is due to the trams it is a varying dc field
that occurs as each tram enters the section and starts to draw power. Field
cancelation systems work best when the field to be cancelled does not vary,
that is it is always the same frequency with always the same amplitude and
shape - we have an IDE Helmholtz coil system that allowed our field
emission TEM/STEM to be used while facilities spent several months looking
for the source of a 40mG pure, constant 60Hz field which suddenly appeared
one winter! This system cost ~$40K ten years ago.

In a previous life I was involved with a dedicated STEM being installed in
the former East Germany. The lab there had tram tracks on one side of the
building with a power feeder for the trams running along another wall. In
this case the installation engineer used a Hall effect probe to detect the
dc field shift and fed the opposite offset into a set of alignment coils.
This worked and stopped the image annoyingly jumping side to side as the
trams entered the section. The cost in this case was a few dollars in parts
and a few hours of labor!

The instrument you want to move is a Tecnai12? If this is used for life
science work at relatively low magnifications ( {100K) then the effect of
the field may not be noticeable. The microscope would not meet performance
specifications but you would not be using it anywhere near that limit. This
would not be ideal, but may be cheaper!

In general the best approach to fields, wether dc or ac is to avoid them!

Good luck

Alan



}
} -----Original Message-----
} X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl]
} Sent: Thursday, February 05, 2009 8:19 AM
} Subject: [Microscopy] viaWWW: How to get rid of a magnetic field
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Interim Associate Director - RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu


==============================Original Headers==============================
15, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:41:23 2009
15, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184])
15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19FfM3I005185
15, 20 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:41:22 -0600
15, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241])
15, 20 -- (authenticated bits=0)
15, 20 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id n19FfLJM004640
15, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
15, 20 -- Mon, 9 Feb 2009 09:41:21 -0600
15, 20 -- Message-Id: {6.2.1.2.2.20090209091207.021d0370-at-mail.uic.edu}
15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
15, 20 -- Date: Mon, 09 Feb 2009 09:41:20 -0600
15, 20 -- To: j.janssen-at-nki.nl, microscopy-at-microscopy.com
15, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
15, 20 -- Subject: Re: [Microscopy] RE: viaWWW: How to get rid of a magnetic field
15, 20 -- In-Reply-To: {200902062355.n16NtCg3008051-at-ns.microscopy.com}
15, 20 -- References: {200902062355.n16NtCg3008051-at-ns.microscopy.com}
15, 20 -- Mime-Version: 1.0
15, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
15, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================



==============================Original Headers==============================
24, 29 -- From A.MARDINLY-at-numonyx.com Mon Feb 9 10:49:48 2009
24, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19Gnko1010897
24, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Feb 2009 10:49:47 -0600
24, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
24, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id A60F414411D;
24, 29 -- Mon, 9 Feb 2009 09:58:29 -0500 (EST)
24, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
24, 29 -- Mon, 9 Feb 2009 11:49:41 -0500
24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 29 -- Content-class: urn:content-classes:message
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Type: text/plain;
24, 29 -- charset="iso-8859-1"
24, 29 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field
24, 29 -- Date: Mon, 9 Feb 2009 11:49:39 -0500
24, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9DDA13B-at-EXDRESBENMX012.numonyx.local}
24, 29 -- In-Reply-To: {200902091551.n19FpEgv024934-at-ns.microscopy.com}
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- Thread-Topic: [Microscopy] viaWWW: How to get rid of a magnetic field
24, 29 -- Thread-Index: AcmKzkF0uyVH5YtkSbqYBaxcHR+RWAAB+Dug
24, 29 -- References: {200902091551.n19FpEgv024934-at-ns.microscopy.com}
24, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
24, 29 -- To: {nicholls-at-uic.edu}
24, 29 -- Cc: {Microscopy-at-Microscopy.com}
24, 29 -- X-OriginalArrivalTime: 09 Feb 2009 16:49:41.0085 (UTC) FILETIME=[66E778D0:01C98AD6]
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19Gnko1010897
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Mon, 9 Feb 2009 12:03:52 -0600
Subject: [Microscopy] Re: viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's beginning to sound as if the mechanical shutter which closes,
opens, then closes again for the exposure has failed. It's a long time
since I've used a 300 but I'm sure I had a similar problem with an
even older AEI 801 and if I remember rightly a lead had become
dislodged from the vicinity of the shutter mechanism at the back of
the microscope column.

A lot of the older shutters have a characteristic noise as the
mechanism works, this may give a clue if it's stopped happening.

If you have an image that is visible before photography and nothing
happens during the exposure time, then the only other thing worth
trying is perhaps experimenting with exposure times to see if it's
just one setting that has failed.

You may well need to contact an engineer so unless you already know
one who services Philips EM300s, this might be a good time to find
someone because you may need him/her again.

Good luck

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
Fleming Building
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: velliyka-at-umdnj.edu

I agree with giving sample blocks, grids, data, etc to the investigators
immediately after completing the project. We cannot/should not use them and
we should not be responsible for their safe keeping. That is the
responsibility of the investigators.

We do backup service project digital images and hold them for a few months.
Then I will write DVDs and store those. We also backup independent user
digital images for a while but periodically ask them to clear all images off
our computers. They are told that they should copy images onto portable
media immediately after each session. We hold no responsibility for loss if
a hard drive fails, etc and will not go to extraordinary effort to reclaim
lost data.

Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: Alan Nicholls {nicholls-at-uic.edu}
} Reply-To: Alan Nicholls {nicholls-at-uic.edu}
} Date: Mon, 9 Feb 2009 09:13:26 -0600
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] viaWWW: Core Facility data/sample storage
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Ru-ching
}
} I agree totally with Randy. Once the project has been completed we expect
} the users to take and store their own specimens. We also expect all users
} to archive and store their own data. We started, 11 years ago, by also
} storing them in digital form before taking them off instrument computers,
} but after a few years the number of CD-R disks was becoming impossible.
} There were only a very few occasions that users asked for the archived
} images so we no longer do it.
}
} Regards
}
} Alan
}
} At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote:
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi Ru-ching,
} }
} } I would strongly recommend having your users take and store their own
} } samples themselves, and the earlier you start having them do it, the better.
} }
} } For years, we had been keeping samples in storage for our clients, until
} } the volume became overwhelming. Samples, grids,blocks, etc. were piled
} } everywhere. We are now in the very slow process of getting the samples
} } back to those researchers who still want them. The problem is that in
} } some cases, especially high-volume users, the resistance to taking their
} } own samples to their own labs is, well, pretty intense. They got too used
} } to having us do it for them, but then what happens is they might come in
} } and want to review a project from years ago. If we can't find the exact
} } set of specimens, blocks, or grids on short notice, it can get dicey.
} }
} } We are considering instituting a storage fee to provide a little nudge to
} } get people to take their own stuff back. Fees are very effective behavior
} } modifiers and can be used not only for this, but for discouraging
} } late-shows or no-shows on scopes, messes being left behind by sloppy
} } users, failure to fill out forms and logbooks, etc. We generally find we
} } only have to charge a behavior-mod fee (BMF) once or twice and that
} } behavior has been modded.
} }
} } Regarding images, we generally archive them here in digital form, which is
} } not a huge problem with large, cheap hard-drives readily available. We
} } used to back up images on CD's and DVD's, but now we generally leave on
} } big hard drives backing up our primary hard drives on the scope
} } computers. That said, we have very rarely been asked to dig up old
} } images, so it may not really be necessary. It would not be unreasonable,
} } in my opinion, to make the user responsible for this aspect of their work,
} } too.
} }
} } Every lab is different and we all operate under different sets of
} } instructions, but this is how we go about things in our facility.
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&
} } NavType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu]
} } Sent: Sunday, February 08, 2009 3:02 PM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: Core Facility data/sample storage
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} } ---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when replying
} } please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver
} } ---------------------------------------------------------------------------
} }
} } Email: rhsia-at-umaryland.edu
} } Name: Ru-ching Hsia
} }
} } Organization: U Maryland
} }
} } Title-Subject: [Filtered] Core Facility data/sample storage
} }
} } Question: Dear all,
} } I have been the director of a new EM Core
} } facility for just more than a year now. Besides
} } dealing with the wide variety of samples and EM
} } projects brought into our facility, I found
} } myself confronted with a huge amount of image
} } data, specimen blocks and grids, much more than
} } I am used to. After keeping all the blocks and
} } grids of each project for a year, we are running
} } out of grid boxes and have not figured out a best
} } storage and tracking system for grids. I know
} } there are many EM facility directors regularly
} } contribute to the List, I would like to find out
} } what are the policies of other EM Core Facilities
} } concerning keeping user/clientís embedded blocks,
} } grids and image data?
} } Do you keep all the blocks and grids after each
} } project or do you give everything back to your
} } clients after the completion of the project?
} } If you keep the grids and blocks, how long do you
} } keep them? What is the best way to store and
} } index the grids from different projects? We find
} } it easier to keep all the grids from one project
} } in one grid box but that means we need more than
} } 50 grid boxes a year.
} } How about the images? Do you keep them all? Is
} } there any particular software that you use to
} } catalogue all the image files?
} } Before I sign off, I would like to thank everyone
} } in the list who generously shares his/her
} } invaluable knowledge here. I was not trained as
} } an electron microscopist, but was landed this job
} } by a total twist of fate. The list and the
} } archive have been a great resource for me. Thank
} } you all.
} } Sincerely,
} } Ru-ching
} }
} }
} } Login Host: 173.64.120.125
} } ---------------------------------------------------------------------------
} }
} }
} } ==============================Original Headers==============================
} } 8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009
} } 8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov
} } [130.202.238.72])
} } 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n18L0v6e017516
} } 8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00
} } -0600
} } 8, 13 -- Mime-Version: 1.0
} } 8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]}
} } 8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030
} } 8, 13 -- To: microscopy-at-microscopy.com
} } 8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver)
} } 8, 13 -- Subject: viaWWW: Core Facility data/sample storage
} } 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} } 8, 13 -- Content-Transfer-Encoding: 8bit
} } 8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n18L0v6e017516
} } ==============================End of - Headers==============================
} }
} }
} } ==============================Original Headers==============================
} } 25, 30 -- From TindallR-at-missouri.edu Mon Feb 9 08:34:08 2009
} } 25, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
} } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} } 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n19EY7ef007304
} } 25, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 08:34:07
} } -0600
} } 25, 30 -- X-IronPort-Anti-Spam-Filtered: true
} } 25, 30 -- X-IronPort-Anti-Spam-Result:
} } ApoEAOnNj0nRauUp/2dsb2JhbADCKiMBAQEHhAWISoJjAQGBNQaFdA
} } 25, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} } ([209.106.229.41])
} } 25, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 09 Feb
} } 2009 08:34:06 -0600
} } 25, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by
} } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } 25, 30 -- Mon, 9 Feb 2009 08:34:06 -0600
} } 25, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } 25, 30 -- Content-class: urn:content-classes:message
} } 25, 30 -- MIME-Version: 1.0
} } 25, 30 -- Content-Type: text/plain;
} } 25, 30 -- charset="iso-8859-1"
} } 25, 30 -- Subject: RE: [Microscopy] viaWWW: Core Facility data/sample storage
} } 25, 30 -- Date: Mon, 9 Feb 2009 08:34:05 -0600
} } 25, 30 -- Message-ID:
} } {91108EF9255B394CBF8B7E3789814A4103CD7DA6-at-UM-XMAIL08.um.umsystem.edu}
} } 25, 30 -- In-Reply-To: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
} } 25, 30 -- X-MS-Has-Attach:
} } 25, 30 -- X-MS-TNEF-Correlator:
} } 25, 30 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample
} } storage
} } 25, 30 -- Thread-Index: AcmKMI0d/imrqTxSTNe2aPrAmz2cEQAkPpmw
} } 25, 30 -- References: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
} } 25, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} } 25, 30 -- To: {rhsia-at-umaryland.edu}
} } 25, 30 -- Cc: {microscopy-at-microscopy.com}
} } 25, 30 -- X-OriginalArrivalTime: 09 Feb 2009 14:34:06.0042 (UTC)
} } FILETIME=[760A6BA0:01C98AC3]
} } 25, 30 -- Content-Transfer-Encoding: 8bit
} } 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n19EY7ef007304
} } ==============================End of - Headers==============================
}
} Alan W Nicholls, PhD
} Interim Associate Director - RRC
} Director of Research Service Facility (Electron Microscopy)
} Research Resources Center - East (M/C 337)
} Room 110 Science and Engineering South Building
} The University of Illinois at Chicago
} 845 West Taylor St
} Chicago, IL 60607-7058
}
} Tel: 312 996 1227
} Fax: 312 996 8091
} Office: Room 110
}
} Web site www.rrc.uic.edu
}
}
} ==============================Original Headers==============================
} 10, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:10:33 2009
} 10, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu
} [128.248.155.184])
} 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n19FAWmG003455
} 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:33 -0600
} 10, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241])
} 10, 20 -- (authenticated bits=0)
} 10, 20 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id
} n19FAVIM026822
} 10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:32 -0600
} 10, 20 -- Message-Id: {6.2.1.2.2.20090209085934.021d0600-at-mail.uic.edu}
} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
} 10, 20 -- Date: Mon, 09 Feb 2009 09:10:30 -0600
} 10, 20 -- To: Microscopy-at-microscopy.com
} 10, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
} 10, 20 -- Subject: Re: [Microscopy] RE: viaWWW: Core Facility data/sample
} storage
} 10, 20 -- In-Reply-To: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
} 10, 20 -- References: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
} 10, 20 -- Mime-Version: 1.0
} 10, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
} 10, 20 -- Content-Transfer-Encoding: 8bit
} ==============================End of - Headers==============================



==============================Original Headers==============================
7, 32 -- From dsherman-at-purdue.edu Mon Feb 9 12:03:51 2009
7, 32 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130])
7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19I3peM009333
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:03:51 -0600
7, 32 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127])
7, 32 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n19I3pNl009425
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500
7, 32 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9])
7, 32 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n19I3ol2002766
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500
7, 32 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 32 -- Mon, 9 Feb 2009 13:03:51 -0500
7, 32 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ;
7, 32 -- Mon, 9 Feb 2009 18:03:50 +0000
7, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119
7, 32 -- Date: Mon, 09 Feb 2009 13:03:49 -0500
7, 32 -- Subject: Re: [Microscopy] viaWWW: Core Facility data/sample storage
7, 32 -- From: Debby Sherman {dsherman-at-purdue.edu}
7, 32 -- To: Alan Nicholls {nicholls-at-uic.edu} ,
7, 32 -- "message to: MSA list" {microscopy-at-microscopy.com}
7, 32 -- Message-ID: {C5B5DA35.3AA90%dsherman-at-exchange.purdue.edu}
7, 32 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample storage
7, 32 -- Thread-Index: AcmK4MIRSkmQsvSMRgyyuluGmbg8tw==
7, 32 -- In-Reply-To: {200902091513.n19FDQRU006632-at-ns.microscopy.com}
7, 32 -- Mime-version: 1.0
7, 32 -- Content-type: text/plain;
7, 32 -- charset="ISO-8859-1"
7, 32 -- X-OriginalArrivalTime: 09 Feb 2009 18:03:51.0185 (UTC) FILETIME=[C35ED810:01C98AE0]
7, 32 -- X-PMX-Version: 5.4.0.320885
7, 32 -- X-PerlMx-Virus-Scanned: Yes
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19I3peM009333
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Mon, 9 Feb 2009 12:04:01 -0600
Subject: [Microscopy] Re: Philips EM300 plate film: no image on negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 9, 2009, at 9:43 AM, malcolm.haswell-at-sunderland.ac.uk wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} It's beginning to sound as if the mechanical shutter which closes,
} opens, then closes again for the exposure has failed. It's a long time
} since I've used a 300 but I'm sure I had a similar problem with an
} even older AEI 801 and if I remember rightly a lead had become
} dislodged from the vicinity of the shutter mechanism at the back of
} the microscope column.
}
} A lot of the older shutters have a characteristic noise as the
} mechanism works, this may give a clue if it's stopped happening.
}
} If you have an image that is visible before photography and nothing
} happens during the exposure time, then the only other thing worth
} trying is perhaps experimenting with exposure times to see if it's
} just one setting that has failed.
}
} You may well need to contact an engineer so unless you already know
} one who services Philips EM300s, this might be a good time to find
} someone because you may need him/her again.
}

Right, this sounds a lot like a problem I had on a TEM once.
Eventually I learned to look at the edge of the viewing screen to see
if the shutter was opening. Dumb me didn't really get how the shutter
was working during the exposure. As Malcolm describes, you should be
able to see the shutter close (dark screen) before the screen goes up,
a brief blink of light when the shutter opens, followed by another
dark screen before the screen goes down.

If the scope is also equipped with a digital camera, there is always
the chance that someone has diddled the shutter circuit and there is a
mistake someplace. It happened to me when a camera was removed and the
wire to the shutter was not taken away.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
10, 44 -- From jkrupp-at-deltacollege.edu Mon Feb 9 12:04:00 2009
10, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
10, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19I40hB009462
10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:04:00 -0600
10, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
10, 44 -- by localhost (Email Security Appliance) with SMTP id EC9F0213F8C_9906AA5B
10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 17:40:53 +0000 (GMT)
10, 44 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
10, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id BE1EC16E67F_9906AA5F
10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 17:40:53 +0000 (GMT)
10, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
10, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
10, 44 -- with ESMTP id 45484822 for Microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:03:58 -0800
10, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
10, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
10, 44 -- ESMTP id KET84200.QE7 for {Microscopy-at-microscopy.com} ; Mon, 9
10, 44 -- Feb 2009 09:48:02 -0800
10, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1])
10, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 7EBAA8F744D3
10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:03:58 -0800 (PST)
10, 44 -- X-Virus-Scanned: amavisd-new at
10, 44 -- X-Spam-Flag: NO
10, 44 -- X-Spam-Score: -2.48
10, 44 -- X-Spam-Level:
10, 44 -- X-Spam-Status: No, score=-2.48 tagged_above=-10 required=6 tests=[AWL=0.019,
10, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1]
10, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
10, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
10, 44 -- with ESMTP id wCI-evphjeCg for {Microscopy-at-microscopy.com} ;
10, 44 -- Mon, 9 Feb 2009 10:03:57 -0800 (PST)
10, 44 -- Received: from [172.20.2.193] (unknown [172.20.2.193])
10, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C16978F73E7F
10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:03:57 -0800 (PST)
10, 44 -- Message-Id: {5A413F68-AC4C-43F0-9A4B-0BE408EAB0BF-at-deltacollege.edu}
10, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
10, 44 -- To: Microscopy-at-microscopy.com
10, 44 -- In-Reply-To: {200902091743.n19HhJ3b006840-at-ns.microscopy.com}
10, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 44 -- Content-Transfer-Encoding: 7bit
10, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3)
10, 44 -- Subject: Re: [Microscopy] Philips EM300 plate film: no image on negatives
10, 44 -- Date: Mon, 9 Feb 2009 10:03:57 -0800
10, 44 -- References: {200902091743.n19HhJ3b006840-at-ns.microscopy.com}
10, 44 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Mon, 9 Feb 2009 13:56:52 -0600
Subject: [Microscopy] TEM; diffraction pattern analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard, Bill,

We need to make some distinctions here. One might hope to extract
from an electron diffraction pattern:
(a) The positions of each diffraction peak. This gives a measure of
the d-spacings (lattice constants) in the crystal
(b) The intensity of each diffraction peak. This may be used through
some form of refinement technique or direct methods to work out the
atomic positions within the lattice.


Within case (a), we should further distinguish absolute from relative
measurements. Absolute measurements occur when we try to determine
the lattice constants of an unknown crystal based on its pattern.
Relative measurements occur when we try to compare measurements within
a single specimen.

It seems to me that the absolute error in measuring a crystal d-
spacing from a transmission electron diffraction pattern in a TEM has
not changed much in the last decade. The major sources of error are
still the same:
(i) The position of the specimen varies slightly from specimen to
specimen. This change results in a change in objective lens strength
for focussed electron diffraction patterns. The change in focus
changes the effective camera length.
(ii) Hysteresis in the ferromagnetic pole pieces of the intermediate
and projector lenses means that the precise camera length is a
function of each of the intermediate lens settings for the last
several changes of image magnification, and image/diffraction modes.
(iii) Distortions associated with off-axis aberrations in the
intermediate and projector lenses are likely to cause errors when
large g's are used to decrease relative error.
For modern microscopes, reasonably well tuned up, this absolute error
is about 1%.

Relative errors measured under the most favorable conditions are also
pretty much what they were ten years ago. In my opinion, the best
measurement of relative error is done using CBED HOLZ lines from a
single crystal of optimum thickness. Changes of lattice constant
within such a crystal (as might occur from alloying) can be detected
at the 10^-5 level. The HOLZ lines are very narrow, so that slight
shifts can be measured very accurately. The dark HOLZ lines in the
forward disc come down the column near the optic axis so that they are
not much affected by off-axis aberrations. One pitfall with CBED is
that dynamical effects may cause HOLZ line shifts for reasons other
than lattice parameter changes.

For less-than optimal conditions, relative error can be minimized by
making the beam very parallel and going to large g. We tend to see
splitting at higher orders (large g) because (i) the peaks are
intrinsically narrower, (ii) the small shifts accumulate, and (iii)
there is less diffuse background. Use of large g reduces relative
errors, but only up to a point. At very high scattering angle the
signal becomes too weak, and off-axis aberrations start to become
important.

In any case, for parallel-beam diffraction, there will ultimately be a
need to separate two closely spaced diffraction maxima. Detectors
with good spatial discrimination are essential. However pixel size in
CCD cameras has not diminished much over the last decade.
Manufacturers assume that you will be able to use your intermediate
and projector lenses to produce adequate magnifications or camera
lengths. Software analysis of diffraction patterns recorded with
digital cameras might give some modest improvement in background
subtraction or peak separation provided that the fundamental signal is
adequate. Using the best available intermediate lenses, digital
cameras, and image analysis software might allow you to achieve
relative errors as low as 1 X 10^-4, but normally one should expect
something somewhat larger, say 5 X 10^-4.



b) Intensity measurements for structure determinations have to deal
with (i) data collection, (ii) dynamical effects, and (iii) the phase
problem in crystallography.
By data collection, I refer to (i) the difficulties encountered in
trying to extract the elastically scattered electrons from the diffuse
background, and (ii) issues of deviation from Bragg angle. One can
energy-filter the diffraction pattern, but that will not eliminate
those electrons that create phonons in the specimen and lose tenths of
eV's. Low T and high KV help but do not totally solve this problem.
Because electron diffraction requires thin specimens, the diffraction
equations are relaxed such that intensity occurs off the Bragg angle.
Measuring the total intensity of a beam requires some form of
integration over a volume of reciprocal space. Off the top of my
head, I'm not aware of anyone trying to do that successfully.
Electrons undergo multiple diffractions (phase shifts greater than
2*pi) within thicker specimens. This means that measured intensity is
not simply related to the square of the structure factor. The
thickness and precise orientation of the specimen have to be factored
in, and they are generally not sufficiently well known. Thus, many of
the techniques developed for weak scatterers, like X-rays, do not
work. This is fundamental physics; technology has not touched it.
Finally, even if we can measure intensity and assume that it is
proportional to the structure factor times its complex conjugate, we
still do not measure the phase of the structure factor. X-ray people
make up for this lack of phase information using a variety of
techniques that, ultimately, rely on the redundancy of diffraction
data. They either create model structures and simulate the
diffraction patterns or (ii) use direct methods.
As Bill mentioned, Dorset and others have managed to overcome these
difficulties and solve some organic structures. In effect they work
under conditions that make electrons behave more like X-rays. At very
high energies, with very-low-atomic-number specimens, electrons are
not likely to be so dynamic, diffuse scatter will be low, and the
deviations from Bragg are not very different among the beams used for
analysis. Under these conditions, the approximations and
calculational algorithms developed for X-rays can work.

For those of us who work with thicker, small-unit-call, higher-atomic-
number, specimens at 200keV, our best hope for structure determination
is to use high resolution images. Aberration correctors and/or focal
series reconstructions may be helpful. But your question was directed
toward electron diffraction...

This has gotten rather long. Perhaps it might compete for the longest
single contribution to the microscopy list server.

(In fact, I'm not sure that I know how to upload this to the list
server. It's well past 5 pm on a Friday; I'm disinclined to remind
myself how to do it. If either of you think this might be of more
general interest, feel free to put it up for me.)

Lew Rabenberg
University of Texas

On Jan 30, 2009, at 12:06 PM, tivol-at-caltech.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} On Jan 30, 2009, at 9:06 AM, contact-at-integrityscientific.com wrote:
}
} } I would like to get some information on TEM diffraction pattern
} } analysis. Specifically;
} }
} } 1) What software is available for analysis of diffraction patterns
} } (both
} } ring patterns and spot patterns)? What kind of accuracy can be
} } obtained
} } - are we getting close to the accuracy of X-ray diffractometry yet,
} } or
} } are there fundamental reasons such as lens aberrations, smaller Bragg
} } angles, and accuracy of measurement which mean that we'll never get
} } there?
} }
} } 2) What are the typical procedures people use for, say, measuring
} } camera
} } length or identifying unknown phases using diffraction?
} }
} Dear Richard,
} I certainly do not know all the existing software, but I do know that
} there is the SP operation in SPIDER that can identify lattice points,
} find the centers and radii of rings, and determine intensity values.
} When I was doing SAED to determine the structure of phthalocyanines, I
} wrote a script that performed a background subtraction in two steps.
} The first step put boxes around all the spots, replaced the pixels
} inside the boxes with values that were the average of the edges of the
} boxes, then took a radial average (the center of which was the center
} of the lattice). This was then subtracted from the ED pattern, which
} got rid of the non-linear background and didn't have to be exact,
} since the second step was a bilinear background subtraction. The
} resulting intensities were sufficiently accurate to give reliable
} structure determinations. I published several articles with Doug
} Dorset and Jim Turner about this in the early '90s. Neither lens
} aberrations, nor small Bragg angles present practical problems for
} structure determinations, but dynamical scattering is a serious issue,
} which was overcome in my work by operating at 1200 kV, which reduced
} dynamical effects to a manageable level. Although I have never done
} any CBED, I have heard reports at M&M detailing the information that
} can be obtained, and these said that the low-order spots gave the most
} accurate data on such features as the distribution of electrons in
} chemical bonds. these data were more accurate than could be obtained
} by any other method, including X-ray diffraction. John Spence is the
} expert on this, so you may want to contact him.
} I evaporated gold onto the phthalocyanines and took SAED patterns
} from which the lattice constants of the phthalocyanines were
} determined. (This also determines the camera length.) Having both
} the gold and the specimen on the same grid controls for changes in
} camera length that may occur with variations in specimen height or
} other scope parameters. The phases were found by direct methods--in
} the case of the phthalocyanines, the Sayre equation and triplet
} formulas were sufficient, but either the tangent formula or maximum
} entropy methods should work also. I authored a paper in Acta Cryst.
} showing that these methods will work for electron diffraction, since
} they are a consequence of the unitary nature of scattering processes.
} The references in that paper are to work done by several people to
} which I added a small amount.
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}

==============================Original Headers==============================
17, 22 -- From tivol-at-caltech.edu Mon Feb 9 13:56:52 2009
17, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19Juqlc013114
17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:56:52 -0600
17, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
17, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id D47CE32A288
17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 11:56:51 -0800 (PST)
17, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
17, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
17, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 46B5E329F96
17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 11:56:50 -0800 (PST)
17, 22 -- Message-Id: {E2EDD153-8281-4558-A305-39155A41037B-at-caltech.edu}
17, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
17, 22 -- To: microscopy-at-microscopy.com
17, 22 -- In-Reply-To: {200901301806.n0UI6rlk028308-at-ns.microscopy.com}
17, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
17, 22 -- Content-Transfer-Encoding: 7bit
17, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
17, 22 -- Subject: Re: [Microscopy] Re: TEM; diffraction pattern analysis
17, 22 -- Date: Mon, 9 Feb 2009 11:56:49 -0800
17, 22 -- References: {200901301806.n0UI6rlk028308-at-ns.microscopy.com}
17, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: dcromey-at-email.arizona.edu
Date: Mon, 9 Feb 2009 14:18:24 -0600
Subject: [Microscopy] Re: viaWWW: Core Facility data/sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Eons ago, when I worked in a clinical TEM lab, we stored everything forever.
To save space and the cost of expensive grid boxes, we would archive the
grids by putting them in labeled small beem capsules, that were stored in
the same white slide box as the tissue blocks. That allowed us to reuse the
grid boxes, which we labeled by writing on scotch tape attached to the
sliding lid.

The research TEM lab here has investigators take their
blocks/grids/slides/negatives back and people are responsible for the
long-term archiving of their digital images.

Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA

office: AHSC 4212 email: Cromey-at-Arizona.edu
voice: 520-626-2824 fax: 520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Monday, February 09, 2009 11:05 AM
To: dcromey-at-email.arizona.edu

I agree with giving sample blocks, grids, data, etc to the investigators
immediately after completing the project. We cannot/should not use them and
we should not be responsible for their safe keeping. That is the
responsibility of the investigators.

We do backup service project digital images and hold them for a few months.
Then I will write DVDs and store those. We also backup independent user
digital images for a while but periodically ask them to clear all images off
our computers. They are told that they should copy images onto portable
media immediately after each session. We hold no responsibility for loss if
a hard drive fails, etc and will not go to extraordinary effort to reclaim
lost data.

Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: Alan Nicholls {nicholls-at-uic.edu}
} Reply-To: Alan Nicholls {nicholls-at-uic.edu}
} Date: Mon, 9 Feb 2009 09:13:26 -0600
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] viaWWW: Core Facility data/sample storage
}
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Ru-ching
}
} I agree totally with Randy. Once the project has been completed we expect
} the users to take and store their own specimens. We also expect all users
} to archive and store their own data. We started, 11 years ago, by also
} storing them in digital form before taking them off instrument computers,
} but after a few years the number of CD-R disks was becoming impossible.
} There were only a very few occasions that users asked for the archived
} images so we no longer do it.
}
} Regards
}
} Alan
}
} At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote:
}
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } Hi Ru-ching,
} }
} } I would strongly recommend having your users take and store their own
} } samples themselves, and the earlier you start having them do it, the
better.
} }
} } For years, we had been keeping samples in storage for our clients, until
} } the volume became overwhelming. Samples, grids,blocks, etc. were piled
} } everywhere. We are now in the very slow process of getting the samples
} } back to those researchers who still want them. The problem is that in
} } some cases, especially high-volume users, the resistance to taking their
} } own samples to their own labs is, well, pretty intense. They got too
used
} } to having us do it for them, but then what happens is they might come in
} } and want to review a project from years ago. If we can't find the exact
} } set of specimens, blocks, or grids on short notice, it can get dicey.
} }
} } We are considering instituting a storage fee to provide a little nudge to
} } get people to take their own stuff back. Fees are very effective
behavior
} } modifiers and can be used not only for this, but for discouraging
} } late-shows or no-shows on scopes, messes being left behind by sloppy
} } users, failure to fill out forms and logbooks, etc. We generally find we
} } only have to charge a behavior-mod fee (BMF) once or twice and that
} } behavior has been modded.
} }
} } Regarding images, we generally archive them here in digital form, which
is
} } not a huge problem with large, cheap hard-drives readily available. We
} } used to back up images on CD's and DVD's, but now we generally leave on
} } big hard drives backing up our primary hard drives on the scope
} } computers. That said, we have very rarely been asked to dig up old
} } images, so it may not really be necessary. It would not be unreasonable,
} } in my opinion, to make the user responsible for this aspect of their
work,
} } too.
} }
} } Every lab is different and we all operate under different sets of
} } instructions, but this is how we go about things in our facility.
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} }
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week
&
} } NavType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu]
} } Sent: Sunday, February 08, 2009 3:02 PM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: Core Facility data/sample storage
} }
} }
} }
} }
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} }
---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when
replying
} } please copy both rhsia-at-umaryland.edu as well as the MIcroscopy
Listserver
} }
---------------------------------------------------------------------------
} }
} } Email: rhsia-at-umaryland.edu
} } Name: Ru-ching Hsia
} }
} } Organization: U Maryland
} }
} } Title-Subject: [Filtered] Core Facility data/sample storage
} }
} } Question: Dear all,
} } I have been the director of a new EM Core
} } facility for just more than a year now. Besides
} } dealing with the wide variety of samples and EM
} } projects brought into our facility, I found
} } myself confronted with a huge amount of image
} } data, specimen blocks and grids, much more than
} } I am used to. After keeping all the blocks and
} } grids of each project for a year, we are running
} } out of grid boxes and have not figured out a best
} } storage and tracking system for grids. I know
} } there are many EM facility directors regularly
} } contribute to the List, I would like to find out
} } what are the policies of other EM Core Facilities
} } concerning keeping user/clientís embedded blocks,
} } grids and image data?
} } Do you keep all the blocks and grids after each
} } project or do you give everything back to your
} } clients after the completion of the project?
} } If you keep the grids and blocks, how long do you
} } keep them? What is the best way to store and
} } index the grids from different projects? We find
} } it easier to keep all the grids from one project
} } in one grid box but that means we need more than
} } 50 grid boxes a year.
} } How about the images? Do you keep them all? Is
} } there any particular software that you use to
} } catalogue all the image files?
} } Before I sign off, I would like to thank everyone
} } in the list who generously shares his/her
} } invaluable knowledge here. I was not trained as
} } an electron microscopist, but was landed this job
} } by a total twist of fate. The list and the
} } archive have been a great resource for me. Thank
} } you all.
} } Sincerely,
} } Ru-ching
} }
} }
} } Login Host: 173.64.120.125
} }
---------------------------------------------------------------------------
} }
} }
} } ==============================Original
Headers==============================
} } 8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009
} } 8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov
} } [130.202.238.72])
} } 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n18L0v6e017516
} } 8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00
} } -0600
} } 8, 13 -- Mime-Version: 1.0
} } 8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]}
} } 8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030
} } 8, 13 -- To: microscopy-at-microscopy.com
} } 8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver)
} } 8, 13 -- Subject: viaWWW: Core Facility data/sample storage
} } 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} } 8, 13 -- Content-Transfer-Encoding: 8bit
} } 8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n18L0v6e017516
} } ==============================End of -
Headers==============================
} }
} }
} } ==============================Original
Headers==============================
} } 25, 30 -- From TindallR-at-missouri.edu Mon Feb 9 08:34:08 2009
} } 25, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
} } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} } 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n19EY7ef007304
} } 25, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 08:34:07
} } -0600
} } 25, 30 -- X-IronPort-Anti-Spam-Filtered: true
} } 25, 30 -- X-IronPort-Anti-Spam-Result:
} } ApoEAOnNj0nRauUp/2dsb2JhbADCKiMBAQEHhAWISoJjAQGBNQaFdA
} } 25, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} } ([209.106.229.41])
} } 25, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 09 Feb
} } 2009 08:34:06 -0600
} } 25, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by
} } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } 25, 30 -- Mon, 9 Feb 2009 08:34:06 -0600
} } 25, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } 25, 30 -- Content-class: urn:content-classes:message
} } 25, 30 -- MIME-Version: 1.0
} } 25, 30 -- Content-Type: text/plain;
} } 25, 30 -- charset="iso-8859-1"
} } 25, 30 -- Subject: RE: [Microscopy] viaWWW: Core Facility data/sample
storage
} } 25, 30 -- Date: Mon, 9 Feb 2009 08:34:05 -0600
} } 25, 30 -- Message-ID:
} } {91108EF9255B394CBF8B7E3789814A4103CD7DA6-at-UM-XMAIL08.um.umsystem.edu}
} } 25, 30 -- In-Reply-To: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
} } 25, 30 -- X-MS-Has-Attach:
} } 25, 30 -- X-MS-TNEF-Correlator:
} } 25, 30 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample
} } storage
} } 25, 30 -- Thread-Index: AcmKMI0d/imrqTxSTNe2aPrAmz2cEQAkPpmw
} } 25, 30 -- References: {200902082102.n18L2RAF019107-at-ns.microscopy.com}
} } 25, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} } 25, 30 -- To: {rhsia-at-umaryland.edu}
} } 25, 30 -- Cc: {microscopy-at-microscopy.com}
} } 25, 30 -- X-OriginalArrivalTime: 09 Feb 2009 14:34:06.0042 (UTC)
} } FILETIME=[760A6BA0:01C98AC3]
} } 25, 30 -- Content-Transfer-Encoding: 8bit
} } 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n19EY7ef007304
} } ==============================End of -
Headers==============================
}
} Alan W Nicholls, PhD
} Interim Associate Director - RRC
} Director of Research Service Facility (Electron Microscopy)
} Research Resources Center - East (M/C 337)
} Room 110 Science and Engineering South Building
} The University of Illinois at Chicago
} 845 West Taylor St
} Chicago, IL 60607-7058
}
} Tel: 312 996 1227
} Fax: 312 996 8091
} Office: Room 110
}
} Web site www.rrc.uic.edu
}
}
} ==============================Original
Headers==============================
} 10, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:10:33 2009
} 10, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu
} [128.248.155.184])
} 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n19FAWmG003455
} 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:33 -0600
} 10, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu
[131.193.156.241])
} 10, 20 -- (authenticated bits=0)
} 10, 20 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id
} n19FAVIM026822
} 10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NOT)
} 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:10:32 -0600
} 10, 20 -- Message-Id: {6.2.1.2.2.20090209085934.021d0600-at-mail.uic.edu}
} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
} 10, 20 -- Date: Mon, 09 Feb 2009 09:10:30 -0600
} 10, 20 -- To: Microscopy-at-microscopy.com
} 10, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
} 10, 20 -- Subject: Re: [Microscopy] RE: viaWWW: Core Facility data/sample
} storage
} 10, 20 -- In-Reply-To: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
} 10, 20 -- References: {200902091435.n19EZYVk008542-at-ns.microscopy.com}
} 10, 20 -- Mime-Version: 1.0
} 10, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
} 10, 20 -- Content-Transfer-Encoding: 8bit
} ==============================End of -
Headers==============================



==============================Original Headers==============================
7, 32 -- From dsherman-at-purdue.edu Mon Feb 9 12:03:51 2009
7, 32 -- Received: from mailhub130.itcs.purdue.edu
(mailhub130.itcs.purdue.edu [128.210.5.130])
7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n19I3peM009333
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:03:51
-0600
7, 32 -- Received: from mailhub127.itcs.purdue.edu
(mailhub127.itcs.purdue.edu [128.210.5.127])
7, 32 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n19I3pNl009425
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51
-0500
7, 32 -- Received: from 1061exfe02a.itap.purdue.edu
(1061exfe02a.itap.purdue.edu [128.210.1.9])
7, 32 -- by mailhub127.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n19I3ol2002766
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51
-0500
7, 32 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by
1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 32 -- Mon, 9 Feb 2009 13:03:51 -0500
7, 32 -- Received: from 128.210.161.94 ([128.210.161.94]) by
EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server
exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV
;
7, 32 -- Mon, 9 Feb 2009 18:03:50 +0000
7, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119
7, 32 -- Date: Mon, 09 Feb 2009 13:03:49 -0500
7, 32 -- Subject: Re: [Microscopy] viaWWW: Core Facility data/sample
storage
7, 32 -- From: Debby Sherman {dsherman-at-purdue.edu}
7, 32 -- To: Alan Nicholls {nicholls-at-uic.edu} ,
7, 32 -- "message to: MSA list" {microscopy-at-microscopy.com}
7, 32 -- Message-ID: {C5B5DA35.3AA90%dsherman-at-exchange.purdue.edu}
7, 32 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample
storage
7, 32 -- Thread-Index: AcmK4MIRSkmQsvSMRgyyuluGmbg8tw==
7, 32 -- In-Reply-To: {200902091513.n19FDQRU006632-at-ns.microscopy.com}
7, 32 -- Mime-version: 1.0
7, 32 -- Content-type: text/plain;
7, 32 -- charset="ISO-8859-1"
7, 32 -- X-OriginalArrivalTime: 09 Feb 2009 18:03:51.0185 (UTC)
FILETIME=[C35ED810:01C98AE0]
7, 32 -- X-PMX-Version: 5.4.0.320885
7, 32 -- X-PerlMx-Virus-Scanned: Yes
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n19I3peM009333
==============================End of - Headers==============================




==============================Original Headers==============================
22, 28 -- From dcromey-at-email.arizona.edu Mon Feb 9 14:18:23 2009
22, 28 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169])
22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19KIMMA027429
22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 14:18:22 -0600
22, 28 -- Received: from gandalfs_amavis (amavis8.email.arizona.edu [10.0.0.236])
22, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0499B9BA7BF
22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 13:18:22 -0700 (MST)
22, 28 -- Received: from RASTER (hepatic1.cba.arizona.edu [128.196.157.6])
22, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 8FCEF9BA7C9
22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 13:18:19 -0700 (MST)
22, 28 -- Reply-To: {cromey-at-arizona.edu}
22, 28 -- From: "Doug Cromey" {dcromey-at-email.arizona.edu}
22, 28 -- To: "Microscopy listserv" {Microscopy-at-Microscopy.Com}
22, 28 -- References: {200902091804.n19I4edN011036-at-ns.microscopy.com}
22, 28 -- In-Reply-To: {200902091804.n19I4edN011036-at-ns.microscopy.com}
22, 28 -- Subject: RE: [Microscopy] Re: viaWWW: Core Facility data/sample storage
22, 28 -- Date: Mon, 9 Feb 2009 13:18:18 -0700
22, 28 -- Organization: University of Arizona
22, 28 -- Message-ID: {003601c98af3$8d324de0$a796e9a0$-at-arizona.edu}
22, 28 -- MIME-Version: 1.0
22, 28 -- Content-Type: text/plain;
22, 28 -- charset="iso-8859-1"
22, 28 -- X-Mailer: Microsoft Office Outlook 12.0
22, 28 -- Thread-Index: AcmK4OQKYRUd5ruSS1yj29DwEiPrLQAEqLTg
22, 28 -- Content-Language: en-us
22, 28 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
22, 28 -- Content-Transfer-Encoding: 8bit
22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19KIMMA027429
==============================End of - Headers==============================




From: twigg-at-estd.nrl.navy.mil
Date: Mon, 9 Feb 2009 14:32:28 -0600
Subject: [Microscopy] viaWWW: Staining Proteins for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both twigg-at-estd.nrl.navy.mil as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: Naval Research Laboratory

Title-Subject: [Filtered] Staining Proteins for TEM

Question: Over a month ago I sent out a message indicating that I
would like to know how to stain proteins for enhanced contrast in TEM
imaging. I have no prior experience with imaging biological samples,
so I would appreciate any helpful comments. I received a number of
helpful suggestions, including the observation that I would benefit
by giving more of the details of my proposed experiment so that the
people could give me advice more specific to my needs.

So, in an attempt to compose a better description of my problem, let
me begin by saying that I am studying the Cytochrome c protein. 12
mg of the protein is dissolved in 1 mL of 100 mM Tris buffer. For
part of this project I would just like to look at a drop of this
solution on a holy carbon grid. So I would like to know how to stain
the proteins that I have deposited on this carbon grid. One of my
collaborators has suggested osmium tetroxide as the staining agent.
I am using a 300 kV TEM to image the proteins.

In the second part of this project I will be trying to image the
proteins within a carbon nanotube array. I have already succeeding
in imaging this structure, as reported in our recent paper (Lebedev
et al. Langmuir 24 (2008) 8871), using the gold nanoparticles
attached to the protein to at least mark the presence of the proteins
within the nanotubes.

For the moment, I would be happy to just image the proteins on a
carbon grid. Latter on I will concern myself with imaging the
proteins within the carbon nanotubes.



Login Host: 132.250.134.121
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Mon Feb 9 14:32:27 2009
11, 11 -- Received: from [192.168.43.136] (msdvpn072.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19KWOLf009491
11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 14:32:26 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c5b6433a8d95-at-[192.168.43.136]}
11, 11 -- Date: Tue, 10 Feb 2009 07:02:23 +1030
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Staining Proteins for TEM
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Mon, 9 Feb 2009 18:04:06 -0600
Subject: [Microscopy] Current Biological SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mansour,

I'm sorry but I don't have any decent images.
I can recommend the following for good pics...

1. A review in the journal Dermatology
Itin, et al. Hair Shaft Abnormalities - Clues to Diagnosis and Treatment.
Dermatology 2005;211:63-71

2. The skin chapter in the following book
Papadimitriou, et al. Diagnostic Ultrastructure of Non-Neoplastic Diseases.


Hi John,
Would it be possible to have these images.
Best regards,
Mansour

-----Original Message-----
X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au]
Sent: Monday, February 09, 2009 8:17 AM
To: Shafei, Mansour A


Hi Andrea,

Our SEM studies of hair are purely morphological.
We look for things like twists, kinks, knots, longitudinal impressions, or
even invaginations of the hair shaft (so-called "bamboo hair").
We also look at the cuticle for signs of loss or fracture.
Hair shaft abnormalities may be associated with skin disorders or inborn
errors of metabolism. Bear in mind that exogenous factors can also affect
hair morphology.

We also look at hair using polarised light which may produce lovely banding
patterns, eg, the disease called trichothiodystrophy produces alternating
light and dark bands under polarised light (so-called "tiger-tail" pattern).
It's thought to be the result of low sulphur content within the hair.

Regards,

John

Hi John,

I am curious about the baby hair you look at. Are you looking for
morphological abnormalities, elemental composition, or both? I have never
heard of this before.

Thanks,
Andrea

Andrea Blake Brothers
Senior Scientist, MMCC
Microscopy & Microanalysis
Characterization Center
andrea.brothers-at-biovail.com

(703) 480-5879 office
(703) 480-5943 fax

BIOVAIL
3701 Concorde Parkway
Chantilly, VA 20151

The information contained in this e-mail message may be privileged and
confidential information and is intended only for the use of the individual
and/or entity identified in the address of this message. If the reader of
this message is not the intended recipient, or an employee or agent
responsible to deliver it to the intended recipient, you are hereby
requested not to distribute or copy this communication. If you have received
this communication in error, please notify us immediately by calling us
collect at (703) 480-6000, or by so advising us by return e-mail. In this
circumstance, we request that you delete the original message from your
system.


-----Original Message-----
X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au]
Sent: Thursday, February 05, 2009 6:01 PM
To: Andrea Brothers

Hi Jon,

We have an old Hitachi S-520 SEM still operational.
Once or twice a year we are asked to look at paediatric hair specimens for
defects. Of course, babies can't tell what's wrong with them so looking at
hair samples can sometimes give you a clue as to what is the problem.
Ie, the hair defect may be one part of a wider syndrome.

Regards,

John Brealey

Senior Medical Scientist, Electron Microscopy Unit

T 08 8222 6612
F 08 8222 6425

www.sapathology.sa.gov.au

SA Pathology (TQEH)
Quality Pathology supporting Training and Research




==============================Original Headers==============================
47, 27 -- From john.brealey-at-imvs.sa.gov.au Mon Feb 9 18:04:05 2009
47, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13])
47, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1A041Hw029815
47, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 18:04:04 -0600
47, 27 -- X-IronPort-AV: E=Sophos;i="4.37,407,1231075800";
47, 27 -- d="scan'208";a="9535711"
47, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88])
47, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 10 Feb 2009 10:33:58 +1030
47, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
47, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id
47, 27 -- 8.1.263.0; Tue, 10 Feb 2009 10:34:45 +1030
47, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au
47, 27 -- (Postfix) with ESMTP id 6A53134DCE for {Microscopy-at-microscopy.com} ; Tue, 10
47, 27 -- Feb 2009 10:33:58 +1030 (CST)
47, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
47, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
47, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
47, 27 -- Subject: SEM of Hair
47, 27 -- Date: Tue, 10 Feb 2009 10:33:58 +1030
47, 27 -- Organization: IMVS
47, 27 -- Message-ID: {000601c98b13$122cdfa0$5954150a-at-41347i}
47, 27 -- MIME-Version: 1.0
47, 27 -- Content-Type: text/plain; charset="us-ascii"
47, 27 -- Content-Transfer-Encoding: 7bit
47, 27 -- X-Mailer: Microsoft Office Outlook 11
47, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
47, 27 -- thread-index: AcmLExIETaDV/NAJTvGug3UrtZtXQg==
==============================End of - Headers==============================




From: minwen-at-u.washington.edu
Date: Mon, 9 Feb 2009 23:07:46 -0600
Subject: [Microscopy] viaWWW: Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both minwen-at-u.washington.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: minwen-at-u.washington.edu
Name: Min Spencer

Organization: UW Seattle

Title-Subject: [Filtered] Cacodylate Buffer

Question: We do the perfusion for EM of brain. We use Cacodylate
Buffer. I knew that the Cacodylate Buffer is not good to the
environment. Can we change to the PBS or other buffers?

Thanks

Min Spencer

Login Host: 128.208.64.65
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Mon Feb 9 23:07:46 2009
8, 11 -- Received: from [203.35.254.75] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1A57XJ4019355
8, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 23:07:40 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c5b6bc04d7d3-at-[192.168.43.136]}
8, 11 -- Date: Tue, 10 Feb 2009 15:37:29 +1030
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: minwen-at-u.washington.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Cacodylate Buffer
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Mon, 9 Feb 2009 23:30:39 -0600
Subject: [Microscopy] Fwd: viaWWW: Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good idea, only it is not PBS but PB. The difference is no NaCl in PB
and more phosphate - usually 0.05-0.1M, but use the same concentration
and pH as in your cacodylate protocol. PB most commonly used for EM
fixation is of both salts being Na salts, often referred to as
Sorensen's. You can look the specifics up in many sources.

"Performance" difference is said to be that cacodylate tends to give a
more clear view (= more extraction), and PB *sometimes* causes a
precipitate, either interacting with UA or for unclear reason.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.


} Email: minwen-at-u.washington.edu
} Name: Min Spencer
}
} Organization: UW Seattle
}
} Title-Subject: [Filtered] Cacodylate Buffer
}
} Question: We do the perfusion for EM of brain. We use Cacodylate
} Buffer. I knew that the Cacodylate Buffer is not good to the
} environment. Can we change to the PBS or other buffers?
}
} Thanks
}
} Min Spencer
}
} Login Host: 128.208.64.65
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Mon Feb 9 23:07:46 2009
} 8, 11 -- Received: from [203.35.254.75] (msdvpn072.msd.anl.gov
} [130.202.238.72])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n1A57XJ4019355
} 8, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009
} 23:07:40 -0600
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c5b6bc04d7d3-at-[192.168.43.136]}
} 8, 11 -- Date: Tue, 10 Feb 2009 15:37:29 +1030
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: minwen-at-u.washington.edu (by way of
} MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Cacodylate Buffer
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
8, 23 -- From vladislav_speransky-at-nih.gov Mon Feb 9 23:30:39 2009
8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1A5Udxa001231
8, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 23:30:39 -0600
8, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id CD97B29055B
8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 00:30:38 -0500 (EST)
8, 23 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
8, 23 -- by compute1.internal (MEProxy); Tue, 10 Feb 2009 00:30:38 -0500
8, 23 -- X-Sasl-enc: G6PHINPA0+O9+eZxcGrNxaFAcTDZVNXkj0Holf6Uzce6 1234243838
8, 23 -- Received: from [192.168.0.5] (pool-173-66-189-48.washdc.fios.verizon.net [173.66.189.48])
8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id 7CE3831375
8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 00:30:38 -0500 (EST)
8, 23 -- Message-Id: {D8C79492-7112-4F51-BCC3-3F53BDDDC8F6-at-nih.gov}
8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
8, 23 -- Subject: Fwd: [Microscopy] viaWWW: Cacodylate Buffer
8, 23 -- Date: Tue, 10 Feb 2009 00:30:37 -0500
8, 23 -- References: {200902100508.n1A58rot020685-at-ns.microscopy.com}
8, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 10 Feb 2009 13:38:55 -0600
Subject: [Microscopy] Re: Microscopy Reference Books/Atlases

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to purchase some good microscopy reference books or atlases. I
know this topic has come up before, but I would like to know what people
recommend.

Thanks!
Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood-at-partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.



==============================Original Headers==============================
9, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:38:54 2009
9, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AJcsPA030977
9, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:38:54 -0600
9, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800";
9, 26 -- d="scan'208";a="59281141"
9, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO PHSXCON1.partners.org) ([132.183.130.40])
9, 26 -- by phsmgmx11.partners.org with ESMTP; 10 Feb 2009 14:38:54 -0500
9, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959);
9, 26 -- Tue, 10 Feb 2009 14:38:53 -0500
9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 26 -- Content-class: urn:content-classes:message
9, 26 -- MIME-Version: 1.0
9, 26 -- Subject: [Microscopy] Re: Microscopy Reference Books/Atlases
9, 26 -- Date: Tue, 10 Feb 2009 14:38:53 -0500
9, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9703E237F1-at-PHSXMB30.partners.org}
9, 26 -- X-MS-Has-Attach:
9, 26 -- X-MS-TNEF-Correlator:
9, 26 -- Thread-Topic: [Microscopy] Re: Microscopy Reference Books/Atlases
9, 26 -- Thread-Index: AcmLtzSGVT+XsttPShyD1IJxxj+s1w==
9, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG}
9, 26 -- To: {Microscopy-at-microscopy.com}
9, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:38:53.0927 (UTC) FILETIME=[34E1F770:01C98BB7]
9, 26 -- Content-Type: text/plain; charset="us-ascii"
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1AJcsPA030977
==============================End of - Headers==============================




From: MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 10 Feb 2009 13:42:41 -0600
Subject: [Microscopy] Re: JB-4 Embedding Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need to use JB-4 embedding resin for a project. I will be embedding and
cross-sectioning cells grown in a polymer. I have never used JB-4 and would
like to hear from members who have used it: protocols, staining, etc.

Thanks!
Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood-at-partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.



==============================Original Headers==============================
8, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:42:41 2009
8, 26 -- Received: from phsmgmx10.partners.org (phsmgmx10.partners.org [155.52.251.101])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AJgfAq003368
8, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:42:41 -0600
8, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800";
8, 26 -- d="scan'208";a="209582819"
8, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO PHSXCON1.partners.org) ([132.183.130.40])
8, 26 -- by phsmgmx10.partners.org with ESMTP; 10 Feb 2009 14:42:41 -0500
8, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959);
8, 26 -- Tue, 10 Feb 2009 14:42:40 -0500
8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 26 -- Content-class: urn:content-classes:message
8, 26 -- MIME-Version: 1.0
8, 26 -- Subject: [Microscopy] Re: JB-4 Embedding Resin
8, 26 -- Date: Tue, 10 Feb 2009 14:42:40 -0500
8, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9703E237F2-at-PHSXMB30.partners.org}
8, 26 -- X-MS-Has-Attach:
8, 26 -- X-MS-TNEF-Correlator:
8, 26 -- Thread-Topic: [Microscopy] Re: JB-4 Embedding Resin
8, 26 -- Thread-Index: AcmLt7v89s6G+kLCREiQOlGR5l1tyA==
8, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG}
8, 26 -- To: {Microscopy-at-microscopy.com}
8, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:42:41.0111 (UTC) FILETIME=[BC4B7E70:01C98BB7]
8, 26 -- Content-Type: text/plain; charset="us-ascii"
8, 26 -- Content-Transfer-Encoding: 8bit
8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1AJgfAq003368
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Tue, 10 Feb 2009 14:53:17 -0600
Subject: [Microscopy] Re: Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gib,

We have a limited number of carbon rods left. As you indicated they
are dark and hard.

For our coated grids we use the highly purified graphite (soft,
silvery gray). It coats in our evaporator at between 18 and 20
amps. It produces a very smooth film for our carbon and formvar coated grids.

We have some left over carbon rods. Let me know.

John Arnott

Disclaimer: Ladd Research sells EM supplies including carbon rods

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 05:41 PM 2/6/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
15, 27 -- From jd-at-laddresearch.com Tue Feb 10 14:53:16 2009
15, 27 -- Received: from anders.electric.net (anders.electric.net [72.35.23.15])
15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AKrFPe029651
15, 27 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 14:53:16 -0600
15, 27 -- Received: from 1LWzbU-00069I-T6 by anders.electric.net with emc1-ok (Exim 4.69)
15, 27 -- (envelope-from {jd-at-laddresearch.com} )
15, 27 -- id 1LWzbU-0006Aq-V3; Tue, 10 Feb 2009 12:53:12 -0800
15, 27 -- Received: by emcmailer; Tue, 10 Feb 2009 12:53:12 -0800
15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
15, 27 -- by anders.electric.net with esmtps (TLSv1:AES256-SHA:256)
15, 27 -- (Exim 4.69)
15, 27 -- (envelope-from {jd-at-laddresearch.com} )
15, 27 -- id 1LWzbU-00069I-T6; Tue, 10 Feb 2009 12:53:12 -0800
15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
15, 27 -- Date: Tue, 10 Feb 2009 15:53:05 -0500
15, 27 -- To: ahlst007-at-umn.edu
15, 27 -- From: jd {jd-at-laddresearch.com}
15, 27 -- Subject: Re: [Microscopy] Carbon Rods
15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
15, 27 -- In-Reply-To: {200902062241.n16MfBwv004470-at-ns.microscopy.com}
15, 27 -- References: {200902062241.n16MfBwv004470-at-ns.microscopy.com}
15, 27 -- Mime-Version: 1.0
15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
15, 27 -- X-Outbound-IP: 216.204.198.170
15, 27 -- X-Env-From: jd-at-laddresearch.com
15, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
15, 27 -- Message-Id: {E1LWzbU-0006Aq-V3-at-anders.electric.net}
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Tue, 10 Feb 2009 15:11:04 -0600
Subject: [Microscopy] Carbon Rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Andrea,

As to your question:

What's the difference between carbon and graphite rods? Do certain
ones make better thin films than others?

Both carbon and graphite are produced in a furnace from carbon powder.

Carbon is formed at about 900 degrees C which results in a harder,
amorphous structure. It has a darker appearance and is
harder. Graphite is formed at about 2500 degrees C which results in
a crystalline structure. It is softer (slippery feeling) and
softer. It writes a bit like a pencil.

We use a highly purified graphite for our thin film. It produces a
smoother film and works well in our evaporator at a current of 18 to 20 amps.

We also have carbon rods and technical grade graphite available. The
technical grade is less pure but works in metal coating and other
less critical applications.

John Arnott

Disclaimer: Ladd Research sells evaporators, coated grids and carbon rods

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com




==============================Original Headers==============================
16, 25 -- From jd-at-laddresearch.com Tue Feb 10 15:11:04 2009
16, 25 -- Received: from cernan.electric.net (cernan.electric.net [72.35.23.19])
16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ALB4t2011391
16, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 15:11:04 -0600
16, 25 -- Received: from 1LWzsl-0007aA-Tm by cernan.electric.net with emc1-ok (Exim 4.69)
16, 25 -- (envelope-from {jd-at-laddresearch.com} )
16, 25 -- id 1LWzsl-0007bQ-W0; Tue, 10 Feb 2009 13:11:03 -0800
16, 25 -- Received: by emcmailer; Tue, 10 Feb 2009 13:11:03 -0800
16, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
16, 25 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256)
16, 25 -- (Exim 4.69)
16, 25 -- (envelope-from {jd-at-laddresearch.com} )
16, 25 -- id 1LWzsl-0007aA-Tm; Tue, 10 Feb 2009 13:11:03 -0800
16, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
16, 25 -- Date: Tue, 10 Feb 2009 16:10:57 -0500
16, 25 -- To: andrea.nans-at-gmail.com
16, 25 -- From: Ladd Research {jd-at-laddresearch.com}
16, 25 -- Subject: Carbon Rods
16, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
16, 25 -- Mime-Version: 1.0
16, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
16, 25 -- X-Outbound-IP: 216.204.198.170
16, 25 -- X-Env-From: jd-at-laddresearch.com
16, 25 -- X-Virus-Status: Scanned by VirusSMART (c)
16, 25 -- Message-Id: {E1LWzsl-0007bQ-W0-at-cernan.electric.net}
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Tue, 10 Feb 2009 17:13:20 -0600
Subject: [Microscopy] Geological Point Counter for Modal Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here's one for the geologists:

James Swift and Son (or their descendents) seem to no longer manufacture their esteemed
Point Counter.

Meiji offer one as an accessory to their range of polarising microsopes, but I haven't found
out yet whether the control/counting unit is electromechanical like the old Swift model, or
electronic.

It seems to me that it should be easy enough to marry an the old Swift one to a PC, with
suitable software and electrical interface to the stepper.

Has anyone done this, or does anyone know of a vendor of the appropriate software and/or
interface?

cheers

Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
9, 26 -- From r.sims-at-auckland.ac.nz Tue Feb 10 17:13:19 2009
9, 26 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ANDFox031924
9, 26 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 17:13:19 -0600
9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1])
9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id CE770198C1
9, 26 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:13:13 +1300 (NZDT)
9, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
9, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
9, 26 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
9, 26 -- with ESMTP id pASNCUXhQZt5 for {microscopy-at-Microscopy.Com} ;
9, 26 -- Wed, 11 Feb 2009 12:13:13 +1300 (NZDT)
9, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id AA1C41987B
9, 26 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:13:13 +1300 (NZDT)
9, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
9, 26 -- To: microscopy-at-Microscopy.Com
9, 26 -- Date: Wed, 11 Feb 2009 12:13:14 +1300
9, 26 -- MIME-Version: 1.0
9, 26 -- Subject: Geological Point Counter for Modal Analysis
9, 26 -- Message-ID: {4992C0DA.7961.CDCF70-at-r.sims.auckland.ac.nz}
9, 26 -- Priority: normal
9, 26 -- X-mailer: Pegasus Mail for Windows (4.41)
9, 26 -- Content-type: text/plain; charset=US-ASCII
9, 26 -- Content-transfer-encoding: 7BIT
9, 26 -- Content-description: Mail message body
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Tue, 10 Feb 2009 17:23:16 -0600
Subject: [Microscopy] Woops! Geological Point Counter for Modal Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Memory lapse!

I forgot that in 2005 I discovered that both appropriate software and a digitally-controlled
stage are, in fact, available from Conwy Valley Systems Ltd, in the UK, so no-one needs to
bring them to my attention.

I will, however, still appreciate hearing from anyone who has successfully interfaced one of
the old Swift stages to a PC

cheers
rtch




On 10 Feb 2009 at 17:14, r.sims-at-auckland.ac.nz wrote:




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Here's one for the geologists:

James Swift and Son (or their descendents) seem to no longer manufacture their esteemed
Point Counter.

Meiji offer one as an accessory to their range of polarising microsopes, but I haven't found
out yet whether the control/counting unit is electromechanical like the old Swift model, or
electronic.

It seems to me that it should be easy enough to marry an the old Swift one to a PC, with
suitable software and electrical interface to the stepper.

Has anyone done this, or does anyone know of a vendor of the appropriate software and/or
interface?

cheers

Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



==============================Original Headers==============================
22, 28 -- From r.sims-at-auckland.ac.nz Tue Feb 10 17:23:15 2009
22, 28 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35])
22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ANNF5h013401
22, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 17:23:15 -0600
22, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
22, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 60D3A48148C
22, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:23:14 +1300 (NZDT)
22, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
22, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
22, 28 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
22, 28 -- with ESMTP id HqDg9Av8c9OQ for {microscopy-at-Microscopy.Com} ;
22, 28 -- Wed, 11 Feb 2009 12:23:14 +1300 (NZDT)
22, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
22, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 4662748142D
22, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:23:14 +1300 (NZDT)
22, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
22, 28 -- To: microscopy-at-Microscopy.Com
22, 28 -- Date: Wed, 11 Feb 2009 12:23:15 +1300
22, 28 -- MIME-Version: 1.0
22, 28 -- Subject: Woops! Geological Point Counter for Modal Analysis
22, 28 -- Message-ID: {4992C333.27967.D6FA3D-at-r.sims.auckland.ac.nz}
22, 28 -- Priority: normal
22, 28 -- In-reply-to: {200902102314.n1ANEMCP000813-at-ns.microscopy.com}
22, 28 -- References: {200902102314.n1ANEMCP000813-at-ns.microscopy.com}
22, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
22, 28 -- Content-type: text/plain; charset=US-ASCII
22, 28 -- Content-transfer-encoding: 7BIT
22, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Tue, 10 Feb 2009 18:31:30 -0600
Subject: [Microscopy] SEM Cooling question.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was just tossing around a few thoughts, and I was was wondering if
anyone has experience cooling two instruments with one chiller. My
thinking is that you should be able to daisy-chain the diffusion pumps
together and achieve proper chilling, but the trade-off would be a
decrease in the required temperature coming out of the chiller. Does
the flow rate have to be augmented somehow as well?

Perhaps the better solution is to put a Y connector in the chiller
lines and run the two in parallel?

--Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
4, 31 -- From kraftpiano-at-gmail.com Tue Feb 10 18:31:30 2009
4, 31 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0VTJp029396
4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:31:30 -0600
4, 31 -- Received: by rv-out-0708.google.com with SMTP id k29so86197rvb.30
4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 16:31:29 -0800 (PST)
4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
4, 31 -- :from:to:content-type:content-transfer-encoding;
4, 31 -- bh=wKBgQ589KW6IQrXye9biIk/WzhipxgGu8sfjdx0bmWY=;
4, 31 -- b=hsp0HV96GZB3nvJ7YkW+rdivO4kcOyywL4lxN44p4VeLazBNFdAx87cTMDVHMfehRj
4, 31 -- vsFAWun8ORtL6LAXG5uDET57r6zJiM3nRPT7Msk5CNT2EIQQh6u6nHhUa69Z+lL59xzG
4, 31 -- QOGf1J6ea0UWea673IAF1YRxnqFZTAddrmoTY=
4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
4, 31 -- :content-transfer-encoding;
4, 31 -- b=igGn5V5s+hHIwnY353+jrgHHVWM7ppUa1nocNAkJQ4UwoQEZLQsF7CH4dJt6+qIfhe
4, 31 -- CTaRtL5U4bA/eubxrKlm80HfXukEm0I0iFzGRoUuzE1x4iRSFqt2+JClg9vOBG/ZoTBr
4, 31 -- aT+95zDnyoTP2Rl5+Zr6mTzz023V070yxKrCc=
4, 31 -- MIME-Version: 1.0
4, 31 -- Received: by 10.141.100.15 with SMTP id c15mr3519rvm.222.1234312289521; Tue,
4, 31 -- 10 Feb 2009 16:31:29 -0800 (PST)
4, 31 -- Date: Tue, 10 Feb 2009 19:31:29 -0500
4, 31 -- Message-ID: {25e2b0d20902101631y3200acd6lf0389f51ef9a2499-at-mail.gmail.com}
4, 31 -- Subject: SEM Cooling question.
4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
4, 31 -- To: microscopy-at-microscopy.com
4, 31 -- Content-Type: text/plain; charset=ISO-8859-1
4, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Tue, 10 Feb 2009 18:46:42 -0600
Subject: [Microscopy] Re: SEM Cooling question.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Justin

I run a few instruments off the one cooler, they are in parallel, with individual flow-control
valves and water-flowmeters.

Remember not to have your cooled water below the dew-point!

cheers
rtch


On 10 Feb 2009 at 18:32, kraftpiano-at-gmail.com wrote:

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I was just tossing around a few thoughts, and I was was wondering if
anyone has experience cooling two instruments with one chiller. My
thinking is that you should be able to daisy-chain the diffusion pumps
together and achieve proper chilling, but the trade-off would be a
decrease in the required temperature coming out of the chiller. Does
the flow rate have to be augmented somehow as well?

Perhaps the better solution is to put a Y connector in the chiller
lines and run the two in parallel?

--Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
12, 28 -- From r.sims-at-auckland.ac.nz Tue Feb 10 18:46:42 2009
12, 28 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35])
12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0kfaV010910
12, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 18:46:41 -0600
12, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 9680D481FDB;
12, 28 -- Wed, 11 Feb 2009 13:46:40 +1300 (NZDT)
12, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
12, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
12, 28 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
12, 28 -- with ESMTP id ERS0R92fPwWI; Wed, 11 Feb 2009 13:46:40 +1300 (NZDT)
12, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
12, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 7963F481FD9;
12, 28 -- Wed, 11 Feb 2009 13:46:40 +1300 (NZDT)
12, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
12, 28 -- To: kraftpiano-at-gmail.com
12, 28 -- Date: Wed, 11 Feb 2009 13:46:41 +1300
12, 28 -- MIME-Version: 1.0
12, 28 -- Subject: Re: [Microscopy] SEM Cooling question.
12, 28 -- CC: microscopy-at-Microscopy.Com
12, 28 -- Message-ID: {4992D6C1.2468.1235DA9-at-r.sims.auckland.ac.nz}
12, 28 -- Priority: normal
12, 28 -- In-reply-to: {200902110032.n1B0WR4c030716-at-ns.microscopy.com}
12, 28 -- References: {200902110032.n1B0WR4c030716-at-ns.microscopy.com}
12, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
12, 28 -- Content-type: text/plain; charset=US-ASCII
12, 28 -- Content-transfer-encoding: 7BIT
12, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 10 Feb 2009 18:57:50 -0600
Subject: [Microscopy] SEM Cooling question.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Justin,
Daisy-chaining is not a good idea. Most systems are also cooling some of
the electronics and that needs to be done with the cooler water. Also,
having very warm water at the top of the second DP (and its water baffle)
would be very counter-productive by allowing a great deal more
back-streaming.

A "Y" is fine as long as you put 2 flowmeters on the outlets so that you can
be sure each instrument is getting the proper flow. If one has a higher
resistance to flow (due to mineral or corrosion build up, or just a
different design), it could end up with insufficient flow.

The biggest question is whether or not the chiller has the cooling capacity
for 2 instruments. A pump can be fairly easy to upgrade, but the BTU
capacity of the chiller is fixed (and sometimes less than advertised). If
you're anywhere near the max for the chiller, in terms of BTUs, either get a
second chiller or get a larger chiller. It's not that unusual to run 2
systems off one chiller, but they must run in parallel with separate
controls and the chiller must have enough BTU capacity.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Tuesday, February 10, 2009 7:34 PM
To: kenconverse-at-qualityimages.biz

I was just tossing around a few thoughts, and I was was wondering if
anyone has experience cooling two instruments with one chiller. My
thinking is that you should be able to daisy-chain the diffusion pumps
together and achieve proper chilling, but the trade-off would be a
decrease in the required temperature coming out of the chiller. Does
the flow rate have to be augmented somehow as well?

Perhaps the better solution is to put a Y connector in the chiller
lines and run the two in parallel?

--Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
4, 31 -- From kraftpiano-at-gmail.com Tue Feb 10 18:31:30 2009
4, 31 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com
[209.85.198.249])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1B0VTJp029396
4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:31:30
-0600
4, 31 -- Received: by rv-out-0708.google.com with SMTP id k29so86197rvb.30
4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 16:31:29
-0800 (PST)
4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 31 -- d=gmail.com; s=gamma;
4, 31 --
h=domainkey-signature:mime-version:received:date:message-id:subject
4, 31 -- :from:to:content-type:content-transfer-encoding;
4, 31 -- bh=wKBgQ589KW6IQrXye9biIk/WzhipxgGu8sfjdx0bmWY=;
4, 31 --
b=hsp0HV96GZB3nvJ7YkW+rdivO4kcOyywL4lxN44p4VeLazBNFdAx87cTMDVHMfehRj
4, 31 --
vsFAWun8ORtL6LAXG5uDET57r6zJiM3nRPT7Msk5CNT2EIQQh6u6nHhUa69Z+lL59xzG
4, 31 -- QOGf1J6ea0UWea673IAF1YRxnqFZTAddrmoTY=
4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
4, 31 -- :content-transfer-encoding;
4, 31 --
b=igGn5V5s+hHIwnY353+jrgHHVWM7ppUa1nocNAkJQ4UwoQEZLQsF7CH4dJt6+qIfhe
4, 31 --
CTaRtL5U4bA/eubxrKlm80HfXukEm0I0iFzGRoUuzE1x4iRSFqt2+JClg9vOBG/ZoTBr
4, 31 -- aT+95zDnyoTP2Rl5+Zr6mTzz023V070yxKrCc=
4, 31 -- MIME-Version: 1.0
4, 31 -- Received: by 10.141.100.15 with SMTP id
c15mr3519rvm.222.1234312289521; Tue,
4, 31 -- 10 Feb 2009 16:31:29 -0800 (PST)
4, 31 -- Date: Tue, 10 Feb 2009 19:31:29 -0500
4, 31 -- Message-ID:
{25e2b0d20902101631y3200acd6lf0389f51ef9a2499-at-mail.gmail.com}
4, 31 -- Subject: SEM Cooling question.
4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
4, 31 -- To: microscopy-at-microscopy.com
4, 31 -- Content-Type: text/plain; charset=ISO-8859-1
4, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




==============================Original Headers==============================
18, 25 -- From kenconverse-at-qualityimages.biz Tue Feb 10 18:57:50 2009
18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0vogI024700
18, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:57:50 -0600
18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
18, 25 -- with ESMTP
18, 25 -- id {20090211005749.FZEM18810.cdptpa-omta02.mail.rr.com-at-Ken} ;
18, 25 -- Wed, 11 Feb 2009 00:57:49 +0000
18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
18, 25 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com}
18, 25 -- Subject: RE: [Microscopy] SEM Cooling question.
18, 25 -- Date: Tue, 10 Feb 2009 19:57:44 -0500
18, 25 -- Message-ID: {4212AD7FF6364BA585A485CE87AE457A-at-Ken}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain;
18, 25 -- charset="us-ascii"
18, 25 -- X-Priority: 3 (Normal)
18, 25 -- X-MSMail-Priority: Normal
18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
18, 25 -- In-Reply-To: {200902110034.n1B0Y520000916-at-ns.microscopy.com}
18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
18, 25 -- Thread-Index: AcmL4HGB81kGdvShRtSDGHtlesxOaAAAXAaw
18, 25 -- Importance: Normal
18, 25 -- Content-Transfer-Encoding: 8bit
18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1B0vogI024700
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 10 Feb 2009 20:03:05 -0600
Subject: [Microscopy] Re: SEM Cooling question.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Justin,

Running the 2 in parallel is better since the cooling water will be at
the same temperature for both systems. The flow rate through a DP
should be set so that the water coming out should be slightly warm to
the touch. I think ours run ~1 gal per minute. Most chillers have
pumps that can supply a fairly large water flow. They then have a
pressure valve which shunts the excess flow back into the tank (kind of
like the fuel pump in your car). An inline flow meter is a really
useful device. It is an immediate indication if you have any kind of
blockage restricting flow (e.g. algae, corrosion, etc). I also put a 1
- 5um cartridge water filter in the waterline just before it enters the
instrument.

Flow through the lenses and electronics is a bit more critical. We
usually set the flow so that the outside temperature of the column is
right at room temperature. You have to balance the flow rate and the
temperature for things to work right. Too high a flow rate (warmer
water) and you get vibration which can affect the image. Too low a flow
rate (cooler water) and the water flow meter will shut the lenses down.
For our TEMs, we fint that a water temperature around 60- 65 deg F is
about right.

Cheers,
Henk

kraftpiano-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was just tossing around a few thoughts, and I was was wondering if
} anyone has experience cooling two instruments with one chiller. My
} thinking is that you should be able to daisy-chain the diffusion pumps
} together and achieve proper chilling, but the trade-off would be a
} decrease in the required temperature coming out of the chiller. Does
} the flow rate have to be augmented somehow as well?
}
} Perhaps the better solution is to put a Y connector in the chiller
} lines and run the two in parallel?
}
} --Justin.
}
} --
} "America believes in education; the average professor earns more money
} in a year than a professional athlete earns in a whole week." Evan
} Esar
}
} ==============================Original Headers==============================
} 4, 31 -- From kraftpiano-at-gmail.com Tue Feb 10 18:31:30 2009
} 4, 31 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249])
} 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0VTJp029396
} 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:31:30 -0600
} 4, 31 -- Received: by rv-out-0708.google.com with SMTP id k29so86197rvb.30
} 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 16:31:29 -0800 (PST)
} 4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 4, 31 -- d=gmail.com; s=gamma;
} 4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
} 4, 31 -- :from:to:content-type:content-transfer-encoding;
} 4, 31 -- bh=wKBgQ589KW6IQrXye9biIk/WzhipxgGu8sfjdx0bmWY=;
} 4, 31 -- b=hsp0HV96GZB3nvJ7YkW+rdivO4kcOyywL4lxN44p4VeLazBNFdAx87cTMDVHMfehRj
} 4, 31 -- vsFAWun8ORtL6LAXG5uDET57r6zJiM3nRPT7Msk5CNT2EIQQh6u6nHhUa69Z+lL59xzG
} 4, 31 -- QOGf1J6ea0UWea673IAF1YRxnqFZTAddrmoTY=
} 4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 4, 31 -- d=gmail.com; s=gamma;
} 4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
} 4, 31 -- :content-transfer-encoding;
} 4, 31 -- b=igGn5V5s+hHIwnY353+jrgHHVWM7ppUa1nocNAkJQ4UwoQEZLQsF7CH4dJt6+qIfhe
} 4, 31 -- CTaRtL5U4bA/eubxrKlm80HfXukEm0I0iFzGRoUuzE1x4iRSFqt2+JClg9vOBG/ZoTBr
} 4, 31 -- aT+95zDnyoTP2Rl5+Zr6mTzz023V070yxKrCc=
} 4, 31 -- MIME-Version: 1.0
} 4, 31 -- Received: by 10.141.100.15 with SMTP id c15mr3519rvm.222.1234312289521; Tue,
} 4, 31 -- 10 Feb 2009 16:31:29 -0800 (PST)
} 4, 31 -- Date: Tue, 10 Feb 2009 19:31:29 -0500
} 4, 31 -- Message-ID: {25e2b0d20902101631y3200acd6lf0389f51ef9a2499-at-mail.gmail.com}
} 4, 31 -- Subject: SEM Cooling question.
} 4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} 4, 31 -- To: microscopy-at-microscopy.com
} 4, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} 4, 31 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
one. Lately it doesn't seem to be working."

==============================Original Headers==============================
7, 26 -- From colijn.1-at-osu.edu Tue Feb 10 20:03:04 2009
7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B2343N007544
7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 20:03:04 -0600
7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
7, 26 -- id {01N5CIUF1V1C8WX0KW-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
7, 26 -- Tue, 10 Feb 2009 21:03:03 -0500 (EST)
7, 26 -- Received: from [192.168.1.104]
7, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
7, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
7, 26 -- with ESMTPA id {01N5CIU8NH388WWFKH-at-ecr6.ohio-state.edu} for
7, 26 -- microscopy-at-microscopy.com; Tue, 10 Feb 2009 21:02:58 -0500 (EST)
7, 26 -- Date: Tue, 10 Feb 2009 21:02:54 -0500
7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
7, 26 -- Subject: Re: [Microscopy] SEM Cooling question.
7, 26 -- In-reply-to: {200902110032.n1B0Wxg7031615-at-ns.microscopy.com}
7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
7, 26 -- To: microscopy-at-microscopy.com
7, 26 -- Message-id: {499231CE.8040208-at-osu.edu}
7, 26 -- MIME-version: 1.0
7, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
7, 26 -- Content-transfer-encoding: 7bit
7, 26 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
7, 26 -- References: {200902110032.n1B0Wxg7031615-at-ns.microscopy.com}
==============================End of - Headers==============================




From: djs49-at-cam.ac.uk
Date: Wed, 11 Feb 2009 06:42:48 -0600
Subject: [Microscopy] Advanced Electron Microscopy School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please note this two week course being held in Scandinavia at the end of
June, beginning of July 2009. It is open to anyone with a background in TEM
and/or STEM.

http://www.fei.com/events/MicroscopySchool/overview.aspx

Closing date for applications is March 20th 2009.

==============================Original Headers==============================
4, 25 -- From djs49-at-hermes.cam.ac.uk Wed Feb 11 06:42:47 2009
4, 25 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BCgkJe013604
4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 06:42:47 -0600
4, 25 -- X-Cam-AntiVirus: not scanned (internal relaying)
4, 25 -- X-Cam-SpamDetails: not scanned
4, 25 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
4, 25 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:47605)
4, 25 -- by ppsw-1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:25)
4, 25 -- with esmtpa (EXTERNAL:djs49) id 1LXEQP-0003QP-66 (Exim 4.70) for Microscopy-at-microscopy.com
4, 25 -- (return-path {djs49-at-hermes.cam.ac.uk} ); Wed, 11 Feb 2009 12:42:45 +0000
4, 25 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk)
4, 25 -- with local (PRAYER:djs49) id 1LXEQP-0005IB-SQ (Exim 4.67) for Microscopy-at-microscopy.com
4, 25 -- (return-path {djs49-at-hermes.cam.ac.uk} ); Wed, 11 Feb 2009 12:42:45 +0000
4, 25 -- Received: from [81.107.41.55] by webmail.hermes.cam.ac.uk
4, 25 -- with HTTP (Prayer-1.3.1); 11 Feb 2009 12:42:45 +0000
4, 25 -- Date: 11 Feb 2009 12:42:45 +0000
4, 25 -- From: Dr Debbie Stokes {djs49-at-cam.ac.uk}
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- Subject: Advanced Electron Microscopy School
4, 25 -- Message-ID: {Prayer.1.3.1.0902111242450.10599-at-hermes-2.csi.cam.ac.uk}
4, 25 -- X-Mailer: Prayer v1.3.1
4, 25 -- Mime-Version: 1.0
4, 25 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1
4, 25 -- Sender: Dr Debbie Stokes {djs49-at-hermes.cam.ac.uk}
==============================End of - Headers==============================




From: RHsia-at-umaryland.edu
Date: Wed, 11 Feb 2009 07:20:34 -0600
Subject: [Microscopy] cryo sample preparation and correlative LM & EM techniques workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

Leica-Microsystems is sponsoring a “Cryo Sample Preparation and Correlative Light and Electron Microscopy Techniques†workshop at the University of Maryland, Baltimore on March 16th & 17th, 2009.

The purpose of this workshop is to discuss the rapidly evolving new techniques for high pressure freezing, freeze substitution and cryo-sectioning. The use of correlative LM and EM techniques will be shown via a “live†video format. There will be a special emphasis on step by step procedures. Topics of discussion will include:

-How to optimize freezing for a wide variety of samples
-Strategies for freeze substitution
-How to cut cryo sections of frozen hydrated and sucrose infiltrated samples
-Live cell imaging
-Tomography

Individuals participating in this session will leave with a working knowledge of these cryo-techniques that they can apply immediately to their own research, whether it involves tomography, cryo-sectioning, EM immuno-labeling, or if they just want to have the best available preservation of cellular fine structure.

It is possible for registered users to try out the equipment with their own samples. Please go to our website (http://www.dental.umaryland.edu/Core-imaging/EMCF%20events/cryo-workshop/Workshop%20announcement ) for the tentative program and registration information.

Participation is FREE for all registered users. Register ASAP (http://www.dental.umaryland.edu/Core-imaging/EMCF%20events/cryo-workshop/Workshop%20announcement ).

We hope to see you there.

Best regards,



Ru-ching
 
Ru-ching Hsia, Ph.D å¤å¦‚è
Associate Professor
Director, Core Imaging Facility
http://www.dental.umaryland.edu/Core-imaging
Department of Microbial Pathogenesis
University of Maryland Dental School
Rm 9202, 650 West Baltimore Street
Baltimore, MD 21201
Tel: 410-706 7992
Fax:: 410-706 0193
E-mail: rhsia-at-umaryland.edu




==============================Original Headers==============================
15, 20 -- From RHsia-at-umaryland.edu Wed Feb 11 07:20:33 2009
15, 20 -- Received: from cits-exch1.campus.umaryland.edu (cits-exch1.campus.umaryland.edu [134.192.1.123])
15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BDKWMn028889
15, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 07:20:33 -0600
15, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
15, 20 -- Content-class: urn:content-classes:message
15, 20 -- MIME-Version: 1.0
15, 20 -- Content-Type: text/plain;
15, 20 -- charset="utf-8"
15, 20 -- Subject: cryo sample preparation and correlative LM & EM techniques workshop
15, 20 -- Date: Wed, 11 Feb 2009 08:20:30 -0500
15, 20 -- Message-ID: {3BBD3EDE51DB2043ABF3E819B1CD532A08B4A939-at-cits-exch1.campus.umaryland.edu}
15, 20 -- X-MS-Has-Attach:
15, 20 -- X-MS-TNEF-Correlator:
15, 20 -- Thread-Topic: cryo sample preparation and correlative LM & EM techniques workshop
15, 20 -- thread-index: AcmMS4GQlva0cVrZSKibsXyLdmQlhg==
15, 20 -- From: "Hsia, Ru-Ching" {RHsia-at-umaryland.edu}
15, 20 -- To: {Microscopy-at-microscopy.com}
15, 20 -- Content-Transfer-Encoding: 8bit
15, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n1BDKWMn028889
==============================End of - Headers==============================




From: hale0007-at-mc.duke.edu
Date: Wed, 11 Feb 2009 09:58:08 -0600
Subject: [Microscopy] EM tech position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



PLEASE DO NOT REPLY TO THIS EMAIL; SEE CONTACT INFORMATION BELOW.
Electron Microscopy Technician Position Available

Location: Duke University Medical Center, Durham, NC

Requirements: BS degree. US citizen or green card. Training and experience
in running electron microscopes, proficiency in cutting ultrathin sections
and performing negative staining. Knowledge of scientific laboratory
operation (making solutions, ordering, typing results, keeping records,
etc.). Clinical laboratory and research experience are a plus.

Laboratory description: The work force consists of the director and 6 EM
technologists who perform pathology (500 samples/year), virology (1000
samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes—2 with
cryo attachments, plus ancillary specimen preparation equipment.

EM Laboratory web site:
http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain

Send resume to:
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Laboratory
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Phone: 919 684-3452
Fax: 919 684-3265
Email: saram-at-duke.edu


==============================Original Headers==============================
9, 28 -- From hale0007-at-mc.duke.edu Wed Feb 11 09:58:07 2009
9, 28 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BFw5dt019310
9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 09:58:07 -0600
9, 28 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54])
9, 28 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n1BFw0Ek2584674
9, 28 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 10:58:00 -0500
9, 28 -- Importance: Normal
9, 28 -- X-Priority: 3 (Normal)
9, 28 -- In-Reply-To:
9, 28 -- References:
9, 28 -- Subject: EM tech position available
9, 28 -- MIME-Version: 1.0
9, 28 -- From: Michael J Hale {hale0007-at-mc.duke.edu}
9, 28 -- To: Microscopy-at-microscopy.com
9, 28 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 28 -- 8.0.2|August 07, 2008) at 02/11/2009 10:57:51,
9, 28 -- MIME-CD complete at 02/11/2009 10:57:51,
9, 28 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 28 -- 8.0.2|August 07, 2008) at 02/11/2009 10:57:59 AM
9, 28 -- Message-ID: {OF57FD88DC.E84078B3-ON8525755A.0057B1B7-8525755A.0057B1D1-at-notes.duke.edu}
9, 28 -- Date: Wed, 11 Feb 2009 10:57:51 -0500
9, 28 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008
9, 28 -- Content-type: text/plain; charset=UTF-8
9, 28 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n1BFw5dt019310
==============================End of - Headers==============================




From: contact-at-integrityscientific.com
Date: Wed, 11 Feb 2009 11:12:25 -0600
Subject: [Microscopy] How many TEM labs are there worldwide?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
first to summarise the thread on electron diffraction - although I
didn't get many replies, I think Lew Rabenberg's reply is an excellent
essay on the subject and summarises the current state of play very well
indeed. Thank you Lew, and Bill Tivol, for your responses.

And now another question. Does anyone have an estimate for how many
active TEM labs there are worldwide (covering all subject areas)? My
guess is 'a few thousand'.. but perhaps someone has a better estimate?

Many thanks

Richard Beanland


==============================Original Headers==============================
5, 28 -- From contact-at-integrityscientific.com Wed Feb 11 11:12:24 2009
5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk (mail-relay-3.csv.warwick.ac.uk [137.205.128.9])
5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BHCLS8003869
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 11:12:23 -0600
5, 28 -- Received: from localhost (localhost [127.0.0.1])
5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHCELS008044
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:14 GMT
5, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk
5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk ([127.0.0.1])
5, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024)
5, 28 -- with LMTP id TLF9uQ5mqozL for {microscopy-at-microscopy.com} ;
5, 28 -- Wed, 11 Feb 2009 17:12:08 +0000 (GMT)
5, 28 -- Received: from [137.205.164.175] (hosts-137-205-164-175 [137.205.164.175])
5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHC8xu007968
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:08 GMT
5, 28 -- X-Envelope-From: contact-at-integrityscientific.com
5, 28 -- Message-ID: {499306E4.1050502-at-integrityscientific.com}
5, 28 -- Date: Wed, 11 Feb 2009 17:12:04 +0000
5, 28 -- From: Richard Beanland {contact-at-integrityscientific.com}
5, 28 -- Reply-To: contact-at-integrityscientific.com
5, 28 -- Organization: Integrity Scientific Ltd
5, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
5, 28 -- MIME-Version: 1.0
5, 28 -- To: microscopy-at-microscopy.com
5, 28 -- Subject: How many TEM labs are there worldwide?
5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 28 -- Content-Transfer-Encoding: 7bit
5, 28 -- X-DCC-Warwick-Metrics: anemone; whitelist
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Wed, 11 Feb 2009 12:25:29 -0600
Subject: [Microscopy] How many TEM labs are there worldwide?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,
I think your estimate is low. The University of Michigan of Ann Arbor has at least 4, probably more EM facilities. The TEM specification narrows it down a little, but we have one Anatomy and Cell biology teaching EM lab, a pay-per use research lab, a Biology EM lab, and a Geology EM lab. Now add all the hospitals, pharmaceutical companies, and some biotech startups and you have quite a list. I'm not even partially qualified to estimate materials EM labs.

How do you want to define "EM lab"? Would larger universities count as one lab, or several?

An EM sales rep might have some better numbers than our guesses, but I'm glad you've brought it up, since I'd like to know what you discover.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: contact-at-integrityscientific.com [mailto:contact-at-integrityscientific.com]
Sent: Wednesday, February 11, 2009 12:27 PM
To: Sobocinski, Gregg

Dear All,
first to summarise the thread on electron diffraction - although I
didn't get many replies, I think Lew Rabenberg's reply is an excellent
essay on the subject and summarises the current state of play very well
indeed. Thank you Lew, and Bill Tivol, for your responses.

And now another question. Does anyone have an estimate for how many
active TEM labs there are worldwide (covering all subject areas)? My
guess is 'a few thousand'.. but perhaps someone has a better estimate?

Many thanks

Richard Beanland


==============================Original Headers==============================
5, 28 -- From contact-at-integrityscientific.com Wed Feb 11 11:12:24 2009
5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk (mail-relay-3.csv.warwick.ac.uk [137.205.128.9])
5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BHCLS8003869
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 11:12:23 -0600
5, 28 -- Received: from localhost (localhost [127.0.0.1])
5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHCELS008044
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:14 GMT
5, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk
5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk ([127.0.0.1])
5, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024)
5, 28 -- with LMTP id TLF9uQ5mqozL for {microscopy-at-microscopy.com} ;
5, 28 -- Wed, 11 Feb 2009 17:12:08 +0000 (GMT)
5, 28 -- Received: from [137.205.164.175] (hosts-137-205-164-175 [137.205.164.175])
5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHC8xu007968
5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:08 GMT
5, 28 -- X-Envelope-From: contact-at-integrityscientific.com
5, 28 -- Message-ID: {499306E4.1050502-at-integrityscientific.com}
5, 28 -- Date: Wed, 11 Feb 2009 17:12:04 +0000
5, 28 -- From: Richard Beanland {contact-at-integrityscientific.com}
5, 28 -- Reply-To: contact-at-integrityscientific.com
5, 28 -- Organization: Integrity Scientific Ltd
5, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
5, 28 -- MIME-Version: 1.0
5, 28 -- To: microscopy-at-microscopy.com
5, 28 -- Subject: How many TEM labs are there worldwide?
5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 28 -- Content-Transfer-Encoding: 7bit
5, 28 -- X-DCC-Warwick-Metrics: anemone; whitelist
==============================End of - Headers==============================


==============================Original Headers==============================
14, 25 -- From greggps-at-umich.edu Wed Feb 11 12:25:29 2009
14, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BIPSHe020393
14, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 12:25:28 -0600
14, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by
14, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Wed, 11 Feb
14, 25 -- 2009 13:25:25 -0500
14, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
14, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
14, 25 -- Date: Wed, 11 Feb 2009 13:25:24 -0500
14, 25 -- Subject: RE: [Microscopy] How many TEM labs are there worldwide?
14, 25 -- Thread-Topic: [Microscopy] How many TEM labs are there worldwide?
14, 25 -- Thread-Index: AcmMbf+uaB3m491ZTKiEUSK2B2IllgABpXQg
14, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA3934018D7625-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
14, 25 -- References: {200902111727.n1BHREdk016647-at-ns.microscopy.com}
14, 25 -- In-Reply-To: {200902111727.n1BHREdk016647-at-ns.microscopy.com}
14, 25 -- Accept-Language: en-US
14, 25 -- Content-Language: en-US
14, 25 -- X-MS-Has-Attach:
14, 25 -- X-MS-TNEF-Correlator:
14, 25 -- acceptlanguage: en-US
14, 25 -- Content-Type: text/plain; charset="us-ascii"
14, 25 -- MIME-Version: 1.0
14, 25 -- Content-Transfer-Encoding: 8bit
14, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BIPSHe020393
==============================End of - Headers==============================




From: p.ingram-at-voice.cellbio.duke.edu
Date: Wed, 11 Feb 2009 12:48:26 -0600
Subject: [Microscopy] Re: How many TEM labs are there worldwide?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

This would probably give you a place to start - if you care to lay
out a few thousand dollars. Or place a phone call and speak very
very nicely to someone there!

http://www.researchandmarkets.com/product/62b701/worldwide_optical_transmission_tem_and_scan


Good luck.

PI
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
Peter Ingram
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu


==============================Original Headers==============================
9, 21 -- From p.ingram-at-voice.cellbio.duke.edu Wed Feb 11 12:48:26 2009
9, 21 -- Received: from smtp.duke.edu (smtp-01.oit.duke.edu [152.3.174.14])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BImOOC002420
9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 12:48:25 -0600
9, 21 -- Received: from smtp.duke.edu (localhost.localdomain [127.0.0.1])
9, 21 -- by localhost (Postfix) with SMTP id 56E08308B4D;
9, 21 -- Wed, 11 Feb 2009 13:48:22 -0500 (EST)
9, 21 -- Received: from [152.3.54.111] (dhcp111.fel.duke.edu [152.3.54.111])
9, 21 -- by smtp.duke.edu (Postfix) with ESMTP id 9D45E308B38;
9, 21 -- Wed, 11 Feb 2009 13:48:21 -0500 (EST)
9, 21 -- Mime-Version: 1.0
9, 21 -- Message-Id: {a05200f04c5b8ccb6847e-at-[152.3.54.111]}
9, 21 -- In-Reply-To: {200902111720.n1BHK98j008617-at-ns.microscopy.com}
9, 21 -- References: {200902111720.n1BHK98j008617-at-ns.microscopy.com}
9, 21 -- Date: Wed, 11 Feb 2009 13:48:00 -0500
9, 21 -- To: contact-at-integrityscientific.com
9, 21 -- From: Peter Ingram {p.ingram-at-voice.cellbio.duke.edu}
9, 21 -- Subject: Re: [Microscopy] How many TEM labs are there worldwide?
9, 21 -- Cc: microscopy-at-microscopy.com
9, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
9, 21 -- X-PMX-Version: 5.4.2.338381, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.11.183434
==============================End of - Headers==============================




From: JJasso1-at-neo.rr.com
Date: Wed, 11 Feb 2009 14:05:20 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both JJasso1-at-neo.rr.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: JJasso1-at-neo.rr.com
Name: Jerry Jasso

Title-Subject: [Filtered] MSA Local Affiliate Contact Information

Question: Dear All,

I am trying to reach the membership committee for Capital District
Microscopy & Microanalysis Society to join this local affiliate.
Unfortunately, the president listed at the MSA website cannot be
reached. Does anyone have this contact information?

Best regards,
Jerry Jasso
(330) 771-7647

Login Host: 71.79.186.96
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 15 -- From zaluzec-at-microscopy.com Wed Feb 11 14:05:20 2009
7, 15 -- Received: from vbn.inter-touch.net ([203.35.254.66])
7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BK5Ijg020054
7, 15 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 14:05:19 -0600
7, 15 -- Received: from [10.5.0.10] (unknown [10.5.0.10])
7, 15 -- by vbn.inter-touch.net (Postfix) with ESMTP id 8146D23194
7, 15 -- for {microscopy-at-microscopy.com} ; Thu, 12 Feb 2009 06:35:15 +1030 (CST)
7, 15 -- Mime-Version: 1.0
7, 15 -- Message-Id: {p06240806c5b8dfea8ed2-at-[10.5.0.10]}
7, 15 -- Date: Thu, 12 Feb 2009 06:35:13 +1030
7, 15 -- To: microscopy-at-microscopy.com
7, 15 -- From: JJasso1-at-neo.rr.com (by way of MicroscopyListserver)
7, 15 -- Subject: [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate
7, 15 -- Contact Information
7, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: terry-at-AsylumResearch.com
Date: Wed, 11 Feb 2009 14:49:42 -0600
Subject: [Microscopy] AFM Applications Scientist Job Opening in the UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Asylum Research is seeking an Applications Scientist to assist current
and potential customers in the use of its Atomic Force Microscopes
(AFM) in the Oxford, UK office. Tasks will include performing
demonstrations of the microscopes based in the Oxford laboratory,
provision of technical support both in the field and office, and
presentation of technical material at customer sites and conferences/
trade shows. The Applications Scientist will also be responsible for
customer equipment installations and assisting with new product
development and applications as dictated by customer requirements.
Travel will be about 20%.

The successful candidate will have a solid academic background (PhD
preferred) and will have a minimum of five years practical and
theoretical AFM experience, preferably encompassing work in the fields
of physics, materials, and/or life sciences. The candidate must have
excellent communications skills, both written and oral, and must be
able to understand customer needs, gain customer feedback and develop
a strong understanding of competitive technology and products.

Asylum Research provides a competitive salary and comprehensive
benefits. Interested candidates should email their resume to jobs-at-AsylumResearch.com
. Please include the job title “Applications Scientist UK” in the
subject line in the email along with salary requirements and references.

==============================Original Headers==============================
3, 14 -- From terry-at-AsylumResearch.com Wed Feb 11 14:49:42 2009
3, 14 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129])
3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BKnexe003079
3, 14 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 14:49:41 -0600
3, 14 -- From: Terry Mehr {terry-at-AsylumResearch.com}
3, 14 -- To: Microscopy-at-microscopy.com
3, 14 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes
3, 14 -- Mime-Version: 1.0 (Apple Message framework v930.3)
3, 14 -- Subject: AFM Applications Scientist Job Opening in the UK
3, 14 -- Date: Wed, 11 Feb 2009 12:49:37 -0800
3, 14 -- X-Mailer: Apple Mail (2.930.3)
3, 14 -- Message-ID: {1LXM1a-0007H4-Iw-at-exchange.AsylumResearch.com}
3, 14 -- Content-Transfer-Encoding: 8bit
3, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BKnexe003079
==============================End of - Headers==============================




From: ALawrence-at-entomology.msstate.edu
Date: Fri, 13 Feb 2009 07:46:02 -0600
Subject: [Microscopy] M&M 2009 5 months and counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Capital District Microscopy & Microanalysis Society, located in the
Albany, NY area, is alive and well. We have just relocated and
re-established our website (www.cdmms.org). Info about the Society, how
to join, and who to contact can be found on the web page. We will get
the information updated on the MSA and MAS (Microbeam Analysis Society)
websites shortly. We welcome all within reach of our venues (and any
beyond as well) to participate/join.

Thanks.

Jim McGee, President-elect, CDMMS

************************************
James J. McGee
BMPC- KAPL
Mail Bin 149
PO Box 1072
Schenectady, NY 12301-1072

Tel: 518-395-4612
Fax: 518-395-4340
email: mcgeejj-at-kapl.gov
************************************

-----Original Message-----
X-from: JJasso1-at-neo.rr.com [mailto:JJasso1-at-neo.rr.com]
Sent: Wednesday, February 11, 2009 3:17 PM
To: McGee, James

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at http://microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both JJasso1-at-neo.rr.com as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: JJasso1-at-neo.rr.com
Name: Jerry Jasso

Title-Subject: [Filtered] MSA Local Affiliate Contact Information

Question: Dear All,

I am trying to reach the membership committee for Capital District
Microscopy & Microanalysis Society to join this local affiliate.
Unfortunately, the president listed at the MSA website cannot be
reached. Does anyone have this contact information?

Best regards,
Jerry Jasso
(330) 771-7647

Login Host: 71.79.186.96
------------------------------------------------------------------------
---

==============================Original
Headers==============================
7, 15 -- From zaluzec-at-microscopy.com Wed Feb 11 14:05:20 2009 7, 15 --
Received: from vbn.inter-touch.net ([203.35.254.66])
7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n1BK5Ijg020054
7, 15 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009
14:05:19 -0600
7, 15 -- Received: from [10.5.0.10] (unknown [10.5.0.10])
7, 15 -- by vbn.inter-touch.net (Postfix) with ESMTP id
8146D23194
7, 15 -- for {microscopy-at-microscopy.com} ; Thu, 12 Feb 2009
06:35:15 +1030 (CST)
7, 15 -- Mime-Version: 1.0
7, 15 -- Message-Id: {p06240806c5b8dfea8ed2-at-[10.5.0.10]}
7, 15 -- Date: Thu, 12 Feb 2009 06:35:13 +1030 7, 15 -- To:
microscopy-at-microscopy.com 7, 15 -- From: JJasso1-at-neo.rr.com (by way of
MicroscopyListserver) 7, 15 -- Subject: [Filtered]
MicroscopyListserverviaWWW: MSA Local Affiliate 7, 15 -- Contact
Information 7, 15 -- Content-Type: text/plain; charset="us-ascii" ;
format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
17, 29 -- From mcgeejj-at-kapl.gov Wed Feb 11 15:23:22 2009
17, 29 -- Received: from ibetpms06.bias2000.bettis.gov (bettis.gov [65.170.176.212])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BLNJ7O018516
17, 29 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 15:23:21 -0600
17, 29 -- Received: from atlas.bias2000.bettis.gov (unverified) by ibetpms06.bias2000.bettis.gov
17, 29 -- (Clearswift SMTPRS 5.2.9) with SMTP id {T8c728f068aac1002861dc4-at-ibetpms06.bias2000.bettis.gov} ;
17, 29 -- Wed, 11 Feb 2009 16:23:17 -0500
17, 29 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830);
17, 29 -- Wed, 11 Feb 2009 16:23:16 -0500
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: RE: [Microscopy] [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate
17, 29 -- Date: Wed, 11 Feb 2009 16:23:16 -0500
17, 29 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB8301E6C06C-at-ibetpex02.bias2000.bettis.gov}
17, 29 -- In-Reply-To: {200902112016.n1BKGlk3029298-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: [Microscopy] [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate
17, 29 -- Thread-Index: AcmMhdPLzHO37QXfSIyYE5AXnbvzcAACCEcg
17, 29 -- References: {200902112016.n1BKGlk3029298-at-ns.microscopy.com}
17, 29 -- From: "McGee, James" {mcgeejj-at-kapl.gov}
17, 29 -- To: {microscopy-at-microscopy.com}
17, 29 -- Cc: {JJasso1-at-neo.rr.com}
17, 29 -- X-OriginalArrivalTime: 11 Feb 2009 21:23:16.0976 (UTC) FILETIME=[F45D2700:01C98C8E]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BLNJ7O018516
==============================End of - Headers==============================

From mailbases-at-mail.ru Wed Feb 11 16:54:56 2009
Return-Path: {mailbases-at-mail.ru}
Received: from google.com ([41.250.228.216])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BMso0n002960
for {microscopylistserverarchive-at-microscopy.com} ; Wed, 11 Feb 2009 16:54:55 -0600
Received: from [162.61.56.83] (HELO google.com)
by bright-hombre.org; Wed, 11 Feb 2009 22:54:49 +0000

Greetings,

To all students (or those of you who might have students) attending the
M&M 2009 meeting in Richmond, please consider the student bursary
program offered by MSA. The purpose of these bursaries is to encourage
students to attend the annual MSA/MAS Microscopy and Microanalysis
meeting, where they can meet and interact with the established
microscopy community while defraying some meeting costs.

The students work for 20 hours (or up to 40 hours) during the meeting
and pre-meeting events and are paid $10 an hour. The jobs involve such
things as providing support in the different symposia (helping with
audio-visual needs, maintaining an attendance count, and helping
speakers set up for their presentation), staffing the MSA Megabooth or
volunteer office, monitoring use of the Internet Café, and helping with
poster set-up and take-down.

Once the final program has been established, each bursary will be
contacted and allowed to choose the times and activities they would like
to work. Many times they end up *working* sessions they would
attend anyway. There is an added bonus of a $10 cash meal allotment for
each morning and/or afternoon sessions worked.

If anyone would like to participate in the bursary program, please
check the *I wish to apply for a student bursary* box in section 2
of the registration form. Bursary space is limited, so sign-up early.
Applicants for the bursaries must be members of MSA or MAS, and enrolled
as students at a recognized educational institution. Don*t forget to
check the MSA website for special discounted hotel rates especially for
students as well as other scholarships to help defray even more meeting
costs.

For those *non-students* we could always use volunteers to help
with the above mentioned meeting activities as well. Although not paid
on an hourly basis as the student bursaries, volunteers do receive some
compensation along with the same cash allotment for meals. Plus they
also have the opportunity to interact more with the microscopy community
as they assist with meeting tasks.

If anyone has any questions about the bursary/volunteer program, or
would like to participate, please contact me.

Amanda Lawrence
Electron Microscope Center
Mississippi State University
662-325-3019
alawrence-at-entomology.msstate.edu




==============================Original Headers==============================
11, 23 -- From ALawrence-at-entomology.msstate.edu Fri Feb 13 07:46:02 2009
11, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DDk1gM001733
11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 07:46:01 -0600
11, 23 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186])
11, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n1DDk0Ku023002
11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL)
11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 07:46:00 -0600
11, 23 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu
11, 23 -- with Novell_GroupWise; Fri, 13 Feb 2009 07:46:00 -0600
11, 23 -- Message-Id: {49952532.B26A.00E6.0-at-entomology.msstate.edu}
11, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
11, 23 -- Date: Fri, 13 Feb 2009 07:45:54 -0600
11, 23 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu}
11, 23 -- To: {microscopy-at-microscopy.com}
11, 23 -- Subject: M&M 2009 5 months and counting
11, 23 -- References: {49951C86020000E600057275-at-mailhost2.groupwise.msstate.edu}
11, 23 -- {49951C96020000E600057278-at-mailhost2.groupwise.msstate.edu}
11, 23 -- {49952532020000E60005728C-at-mailhost2.groupwise.msstate.edu}
11, 23 -- Mime-Version: 1.0
11, 23 -- Content-Type: text/plain; charset=ISO-8859-15
11, 23 -- Content-Transfer-Encoding: 8bit
11, 23 -- Content-Disposition: inline
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Fri, 13 Feb 2009 10:43:04 -0600
Subject: [Microscopy] Re: Microscopy Reference Books/Atlases

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Peggy,

No-one seems to have replied so:-


In terms of the operation of the optical microscope so much is on-line is
these days, see our web-links:
http://www.well.ox.ac.uk/cytogenetics/websites.shtml

Not sure what type of books you mean, but for fun coffee table reading [in a
school sixth form or path lab anyway] try

Inside the body - Fantastic images from beneath the skin by Susan Greenfield
(Foreword)

Unseen companions - Big views of tiny creature by Adrian Warren, David
Spears, and Madeleine Spears

I use these occasionally for schools outreach programs.



Plus there's the microscope to telescope images from:

Heaven and Earth: Unseen by the Naked Eye (Photography)

Microcosmos: Discovering the World Through Microscopic Images from 40x to
100,000x Magnification

But these above two books are a bit thick with tiddly photo-sizes for the
superb images contained.



Our web-site has a few 'serious' book suggestions but these are all for
light microscopy [as that's what we do].
http://www.well.ox.ac.uk/cytogenetics/reading.shtml


Keith


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


-----Original Message-----
X-from: MSHERWOOD-at-PARTNERS.ORG [mailto:MSHERWOOD-at-PARTNERS.ORG]
Sent: 10 February 2009 19:48
To: kjmorris-at-well.ox.ac.uk


We are looking to purchase some good microscopy reference books or atlases.
I
know this topic has come up before, but I would like to know what people
recommend.

Thanks!
Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood-at-partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in
error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.



==============================Original Headers==============================
9, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:38:54 2009
9, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org
[155.52.251.65])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1AJcsPA030977
9, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:38:54
-0600
9, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800";
9, 26 -- d="scan'208";a="59281141"
9, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO
PHSXCON1.partners.org) ([132.183.130.40])
9, 26 -- by phsmgmx11.partners.org with ESMTP; 10 Feb 2009 14:38:54 -0500
9, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by
PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959);
9, 26 -- Tue, 10 Feb 2009 14:38:53 -0500
9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 26 -- Content-class: urn:content-classes:message
9, 26 -- MIME-Version: 1.0
9, 26 -- Subject: [Microscopy] Re: Microscopy Reference Books/Atlases
9, 26 -- Date: Tue, 10 Feb 2009 14:38:53 -0500
9, 26 -- Message-ID:
{073AE2BEA1C2BA4A8837AB6C4B943D9703E237F1-at-PHSXMB30.partners.org}
9, 26 -- X-MS-Has-Attach:
9, 26 -- X-MS-TNEF-Correlator:
9, 26 -- Thread-Topic: [Microscopy] Re: Microscopy Reference Books/Atlases
9, 26 -- Thread-Index: AcmLtzSGVT+XsttPShyD1IJxxj+s1w==
9, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG}
9, 26 -- To: {Microscopy-at-microscopy.com}
9, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:38:53.0927 (UTC)
FILETIME=[34E1F770:01C98BB7]
9, 26 -- Content-Type: text/plain; charset="us-ascii"
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1AJcsPA030977
==============================End of - Headers==============================


==============================Original Headers==============================
37, 22 -- From kjmorris-at-well.ox.ac.uk Fri Feb 13 10:43:04 2009
37, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
37, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DGh3ZC023627
37, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Feb 2009 10:43:03 -0600
37, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
37, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
37, 22 -- id 1LY183-0001mZ-20
37, 22 -- for Microscopy-at-Microscopy.Com; Fri, 13 Feb 2009 16:43:03 +0000
37, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
37, 22 -- To: {Microscopy-at-Microscopy.Com}
37, 22 -- References: {200902101947.n1AJlorf019372-at-ns.microscopy.com}
37, 22 -- Subject: RE: [Microscopy] Re: Microscopy Reference Books/Atlases
37, 22 -- Date: Fri, 13 Feb 2009 16:43:02 -0000
37, 22 -- Message-ID: {A3C150EB9B6744019DB9AD177D8EE350-at-CytoWhizz}
37, 22 -- MIME-Version: 1.0
37, 22 -- Content-Type: text/plain;
37, 22 -- charset="us-ascii"
37, 22 -- Content-Transfer-Encoding: 7bit
37, 22 -- X-Mailer: Microsoft Office Outlook 11
37, 22 -- In-Reply-To: {200902101947.n1AJlorf019372-at-ns.microscopy.com}
37, 22 -- Thread-Index: AcmLuHUAwJOCNNBUQhqqqOk/YbZrZACMcWKw
37, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
==============================End of - Headers==============================




From: Matt.Laframboise-at-Kraft.com
Date: Fri, 13 Feb 2009 12:23:49 -0600
Subject: [Microscopy] Job Opportunity: Scientist, Physical Characterization, Tarrytown, NY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scientist-Physical Characterization, Analytical Sciences

Kraft Foods Global, Inc. is currently seeking Scientist-Physical
Characterization, Analytical Sciences to work within our Tarrytown, NY
facility.

Key responsibilities include the following:
* Leverage a strong multidisciplinary scientific background and powder
characterization expertise to support various R&D projects for a broad
range of food systems
* Apply current and develop new and innovative techniques to
characterize and solve practical problems related to food powders
* Develop a fundamental understanding of food powder properties to
provide solutions in the areas of ingredients, formulation, processing,
product quality and product performance: powder stability (e.g.: caking,
sorption isotherms, glass transition), dispersion in liquids,
flowability, segregation/demixing, dusting, process monitoring/control
and mixing phenomena.
* Develop and apply a broad physical characterization expertise
* This position will be located in Tarrytown, NY and will require travel
1 day per week to our East Hanover, NJ facility.
* Build strong working relationships with clients/customers and build
projects and programs to serve their needs
* Build, maintain and leverage strong internal and external network of
technical experts and resources to speedily execute and serve project
needs
* Collaborate across R&D as a cross-functional team member and develop
technical leadership
* Communicate effectively technical findings to project team and
management
Qualified candidates should possess the following:
* MS or PhD in Science or Engineering with a strong proven scientific
and experimental background in powder characterization
* 0-3 years of experience in powder characterization, including thermal
analysis, powder flow and caking, powder surface chemistry and particle
size & shape, with a passion to develop & apply scientific findings to
solve practical problems
* Demonstrated in-depth multidisciplinary scientific knowledge in two or
more of the following areas: Physical Chemistry, Interfacial Colloid
Science, Polymer Science & Process Engineering
* Breadth and depth of scientific knowledge in physical characterization
and the ability to develop new areas of expertise quickly and
effectively
* Ability to build and speedily invoke recent scientific advances and
global network of experts and resources to rapidly advance projects
* Strong and proven interpersonal skills, with the ability to build
strong customer/client relationships
* Ability to work together in cross-functional teams in various roles
* Excellent project management, written and verbal communication skills

If you are interested in applying for this opportunity, please cut and
paste the following link into your web browser to apply directly via the
www.kraftfoods.com/careers web site:

https://www.kraftcareers.com/JobSearch/JobCenterViewCndt.asp?JobAd_Id=93
1944

Or send your resume to:
Matt LaFramboise
Kraft R&D Recruiter
Matt.laframboise-at-kraft.com


Kraft Foods Global, Inc. is an equal opportunity employer m/f/d/v


==============================Original Headers==============================
10, 26 -- From Matt.Laframboise-at-Kraft.com Fri Feb 13 12:23:49 2009
10, 26 -- Received: from mail3.kraft.com (mail3.kraft.com [206.228.222.62])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DINnkg009117
10, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 12:23:49 -0600
10, 26 -- X-IronPort-AV: E=McAfee;i="5300,2777,5525"; a="94689248"
10, 26 -- Received: from unknown (HELO kftusoktulxbh03.KRFT.Net) ([10.53.184.16])
10, 26 -- by mail3.kraft.com with ESMTP; 13 Feb 2009 13:22:48 -0500
10, 26 -- Received: from KFTUSPAWBAXMB31.KRFT.Net ([10.53.184.64]) by kftusoktulxbh03.KRFT.Net with Microsoft SMTPSVC(6.0.3790.3959);
10, 26 -- Fri, 13 Feb 2009 12:22:16 -0600
10, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 26 -- Content-class: urn:content-classes:message
10, 26 -- MIME-Version: 1.0
10, 26 -- Content-Type: text/plain;
10, 26 -- charset="us-ascii"
10, 26 -- Subject: Job Opportunity: Scientist, Physical Characterization, Tarrytown, NY
10, 26 -- Date: Fri, 13 Feb 2009 13:22:15 -0500
10, 26 -- Message-ID: {0A7A37452676694E97DBE0232CF6FA3A66F44F-at-KFTUSPAWBAXMB31.KRFT.Net}
10, 26 -- X-MS-Has-Attach:
10, 26 -- X-MS-TNEF-Correlator:
10, 26 -- Thread-Topic: Job Opportunity: Scientist, Physical Characterization, Tarrytown, NY
10, 26 -- Thread-Index: AcmOB/8C14pRXSQ9QWS6hax5SrLxgg==
10, 26 -- From: {Matt.Laframboise-at-Kraft.com}
10, 26 -- To: {Microscopy-at-microscopy.com}
10, 26 -- X-OriginalArrivalTime: 13 Feb 2009 18:22:16.0310 (UTC) FILETIME=[FFBA8960:01C98E07]
10, 26 -- Content-Transfer-Encoding: 8bit
10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1DINnkg009117
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Fri, 13 Feb 2009 15:16:09 -0600
Subject: [Microscopy] RMC MT6000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm looking for a trimming stand for a MT6000. Does anyone have one
floating around that I could have/buy or know where I might get one?

Thanks,

Andy Bowling



---------------------------
Andrew J. Bowling, PhD
Dow AgroSciences
9330 Zionsville Rd
Indianapolis, IN 46268
317-337-3878
ajbowling-at-dow.com





==============================Original Headers==============================
10, 31 -- From AJBowling-at-dow.com Fri Feb 13 15:16:08 2009
10, 31 -- Received: from mail64.messagelabs.com (mail64.messagelabs.com [216.82.249.227])
10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DLG8nT029797
10, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 15:16:08 -0600
10, 31 -- X-VirusChecked: Checked
10, 31 -- X-Env-Sender: AJBowling-at-dow.com
10, 31 -- X-Msg-Ref: server-10.tower-64.messagelabs.com!1234559766!80653857!1
10, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,-
10, 31 -- X-Originating-IP: [216.99.65.24]
10, 31 -- Received: (qmail 16668 invoked from network); 13 Feb 2009 21:16:07 -0000
10, 31 -- Received: from mail2.dow.com (HELO USMDLMDOWS002.dow.com) (216.99.65.24)
10, 31 -- by server-10.tower-64.messagelabs.com with RC4-SHA encrypted SMTP; 13 Feb 2009 21:16:07 -0000
10, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USMDLMDOWS002.dow.com with Microsoft SMTPSVC(6.0.3790.1830);
10, 31 -- Fri, 13 Feb 2009 16:16:06 -0500
10, 31 -- Content-class: urn:content-classes:message
10, 31 -- MIME-Version: 1.0
10, 31 -- Content-Type: text/plain;
10, 31 -- charset="us-ascii"
10, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 31 -- Subject: RMC MT6000
10, 31 -- Date: Fri, 13 Feb 2009 16:16:05 -0500
10, 31 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1D4A629-at-USMDLMDOWX032.dow.com}
10, 31 -- X-MS-Has-Attach:
10, 31 -- X-MS-TNEF-Correlator:
10, 31 -- Thread-Topic: RMC MT6000
10, 31 -- Thread-Index: AcmOIEf5idvi380ATTex6HOsUPOzYw==
10, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
10, 31 -- To: {microscopy-at-microscopy.com}
10, 31 -- X-OriginalArrivalTime: 13 Feb 2009 21:16:06.0319 (UTC) FILETIME=[487F9BF0:01C98E20]
10, 31 -- Content-Transfer-Encoding: 8bit
10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1DLG8nT029797
==============================End of - Headers==============================




From: wall1-at-llnl.gov
Date: Fri, 13 Feb 2009 19:22:29 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Image J pluins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wall1-at-llnl.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wall1-at-llnl.gov
Name: Mark Wall

Organization: Lawrence Livermore National Lab

Title-Subject: [Filtered] Image J pluins

Question: Is anyone willing to help me off-line on using Image
plugins for making stacks and using other plugins, such as Z-stack
and stack focuser. It appears that I get a Java errors when trying to
us the stack focuser on a created stack.

thank you in advance,

Mark Wall
925 423-7162


Login Host: 128.115.27.11
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri Feb 13 19:22:28 2009
9, 11 -- Received: from [10.128.101.156] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1E1MOhq018216
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 19:22:26 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c5bbcd4113a8-at-[10.5.0.16]}
9, 11 -- Date: Sat, 14 Feb 2009 12:22:21 +1100
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: wall1-at-llnl.gov (by way of MicroscopyListserver)
9, 11 -- Subject: [Filtered] MicroscopyListserverviaWWW: Image J pluins
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 16 Feb 2009 09:34:53 -0600
Subject: [Microscopy] SEM: Breaking wafers accurately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

This has been the year of challenging projects and the year is still
young. Now another one has come over the transom.

One of our researchers is experimenting with photo-resist patterns on
6-inch silicon wafers. Our task is to image cross-sections of tiny
little lines contained in rows of tiny little dots about the size of TEM
grids. Imaging mags are around 100-200kX, so we're talking little here.
Getting these wafers to break accurately is proving to be a real bear
and it may be that the break is distorting the cross-sections.

I'm using a diamond scribe to score the wafers as accurately as I can,
but scribing the back of the wafer to get an accurate break on the front
is proving to be difficult.

There must be lots of industry folks out there who do this on a daily
basis. Would someone be willing to share a few tiny little hints?

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009
10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GFYqGp008710
10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53 -0600
10, 27 -- X-IronPort-Anti-Spam-Filtered: true
10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG
10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb 2009 09:34:50 -0600
10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600
10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 27 -- Content-class: urn:content-classes:message
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain;
10, 27 -- charset="us-ascii"
10, 27 -- Subject: SEM: Breaking wafers accurately
10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600
10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM-XMAIL08.um.umsystem.edu}
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- Thread-Topic: SEM: Breaking wafers accurately
10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww==
10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 27 -- To: {microscopy-at-microscopy.com}
10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC) FILETIME=[1B6248B0:01C9904C]
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1GFYqGp008710
==============================End of - Headers==============================




From: jwheckman-at-earthlink.net
Date: Mon, 16 Feb 2009 10:07:39 -0600
Subject: [Microscopy] RE: SEM: Breaking wafers accurately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy-

I more than occasionally make cleaves through Si wafers, with several types
of resist, with the intent of cross sectioning very small features for SEM.
Typically these are scribed on the front side with a diamond scribe and
cleaved with polymer glaziers-pliers. If need to hit a very small
(sub-micron) feature exactly, I make a series of reductional cleaves
winding up with a SELA microcleaver to get the final cross section. Resist
is one of the harder samples from which to get a good, undistorted
specimen. Then you need to get an undistorted image.... I don't cleave TEM
samples.

Hope this helps,

John


} [Original Message]
} From: {TindallR-at-missouri.edu}
} To: {jwheckman-at-earthlink.net}
} Date: 2/16/2009 8:41:57 AM
} Subject: [Microscopy] SEM: Breaking wafers accurately
}
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Dear Collective,
}
} This has been the year of challenging projects and the year is still
} young. Now another one has come over the transom.
}
} One of our researchers is experimenting with photo-resist patterns on
} 6-inch silicon wafers. Our task is to image cross-sections of tiny
} little lines contained in rows of tiny little dots about the size of TEM
} grids. Imaging mags are around 100-200kX, so we're talking little here.
} Getting these wafers to break accurately is proving to be a real bear
} and it may be that the break is distorting the cross-sections.
}
} I'm using a diamond scribe to score the wafers as accurately as I can,
} but scribing the back of the wafer to get an accurate break on the front
} is proving to be difficult.
}
} There must be lots of industry folks out there who do this on a daily
} basis. Would someone be willing to share a few tiny little hints?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original
Headers==============================
} 10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009
} 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
(mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1GFYqGp008710
} 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53
-0600
} 10, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 10, 27 -- X-IronPort-Anti-Spam-Result:
ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG
} 10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
([209.106.229.41])
} 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb 2009
09:34:50 -0600
} 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by
um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600
} 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 10, 27 -- Content-class: urn:content-classes:message
} 10, 27 -- MIME-Version: 1.0
} 10, 27 -- Content-Type: text/plain;
} 10, 27 -- charset="us-ascii"
} 10, 27 -- Subject: SEM: Breaking wafers accurately
} 10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600
} 10, 27 -- Message-ID:
{91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM-XMAIL08.um.umsystem.edu}
} 10, 27 -- X-MS-Has-Attach:
} 10, 27 -- X-MS-TNEF-Correlator:
} 10, 27 -- Thread-Topic: SEM: Breaking wafers accurately
} 10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww==
} 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 10, 27 -- To: {microscopy-at-microscopy.com}
} 10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC)
FILETIME=[1B6248B0:01C9904C]
} 10, 27 -- Content-Transfer-Encoding: 8bit
} 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1GFYqGp008710
} ==============================End of -
Headers==============================



==============================Original Headers==============================
8, 24 -- From jwheckman-at-earthlink.net Mon Feb 16 10:07:39 2009
8, 24 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GG7dIn023416
8, 24 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 10:07:39 -0600
8, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 24 -- s=dk20050327; d=earthlink.net;
8, 24 -- b=Fi17hy5cKxlM4gPrgseI2DKwqgrk5+gNRePctuDqNMfbP0GzmaxDGgY5R87gUd2/;
8, 24 -- h=Received:Message-ID:X-Priority:Reply-To:X-Mailer:From:To:Subject:Date:MIME-Version:Content-type:X-ELNK-Trace:X-Originating-IP;
8, 24 -- Received: from [173.25.22.129] (helo=earthlink.net)
8, 24 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
8, 24 -- (envelope-from {jwheckman-at-earthlink.net} )
8, 24 -- id 1LZ60Q-0006hJ-Ic; Mon, 16 Feb 2009 11:07:38 -0500
8, 24 -- Message-ID: {380-2200921161686609-at-earthlink.net}
8, 24 -- X-Priority: 3
8, 24 -- Reply-To: jwheckman-at-earthlink.net
8, 24 -- X-Mailer: EarthLink MailBox 2005.3.14.0 (Windows)
8, 24 -- From: "John Heckman" {jwheckman-at-earthlink.net}
8, 24 -- To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} , microscopy-at-microscopy.com
8, 24 -- Subject: RE: [Microscopy] SEM: Breaking wafers accurately
8, 24 -- Date: Mon, 16 Feb 2009 09:08:06 -0700
8, 24 -- MIME-Version: 1.0
8, 24 -- Content-type: text/plain; charset=US-ASCII
8, 24 -- X-ELNK-Trace: 535e151077d6e67c85338a7d01cb3b6a7e972de0d01da9402928fed5e8eb26326fb49aaeafadd567350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
8, 24 -- X-Originating-IP: 173.25.22.129
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Mon, 16 Feb 2009 13:31:51 -0600
Subject: [Microscopy] TEM: diffraction pattern question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Some of our SAD diffraction patterns are showing rings that are
discontinuous. Only a small segment of the rings are sharp (say, a
wedge comprising 25% of the circle) and they taper off to
nothingness, so that directly opposite the sharpest rings, we see
nothing at all. I was wondering what might be causing this (uneven
specimen thickness, specimen tilted, illumination misalignment,
astigmatism). When we diffract a gold standard, no problem, so I
conclude that it must be specimen related.

Any ideas or suggestions to help us solve this SAD situation (sorry,
I couldn't resist....)?
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
4, 17 -- From bozzola-at-siu.edu Mon Feb 16 13:31:51 2009
4, 17 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GJVpLR012804
4, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:31:51 -0600
4, 17 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
4, 17 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n1GJVngs024603
4, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:31:50 -0600 (CST)
4, 17 -- Mime-Version: 1.0
4, 17 -- Message-Id: {a06240801c5bf6d5388ee-at-[131.230.177.136]}
4, 17 -- Date: Mon, 16 Feb 2009 13:31:54 -0600
4, 17 -- To: Microscopy-at-microscopy.com
4, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
4, 17 -- Subject: TEM: diffraction pattern question
4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 17 -- X-Spam-Score: 0.00%
4, 17 -- X-MASF: 0.00%
4, 17 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: jtugglenator-at-gmail.com
Date: Mon, 16 Feb 2009 13:44:33 -0600
Subject: [Microscopy] viaWWW: TEM on Antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jtugglenator-at-gmail.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: jtugglenator-at-gmail.com
Name: Jay Tuggle

Title-Subject: [Filtered] TEM on Antibodies

Question: Wondered if anyone had a fairly simple and effective method
for looking a antibodies on the TEM. Most of the sources i have are
pretty old and i think out dated.

Login Host: 204.119.248.137
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Mon Feb 16 13:44:33 2009
5, 11 -- Received: from [130.194.133.34] ([203.153.194.12])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GJiUUq026877
5, 11 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:44:32 -0600
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c5bf728e383b-at-[130.194.133.34]}
5, 11 -- Date: Tue, 17 Feb 2009 06:44:27 +1100
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: jtugglenator-at-gmail.com (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: TEM on Antibodies
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Mon, 16 Feb 2009 14:08:20 -0600
Subject: [Microscopy] TEM of bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bacteria can be difficult to infiltrate with resin. Even using Spurr's, and
increased infiltration times, I often end up with holes in the center of
some of the bacteria. Are there any good tricks for improving the
infiltration of bacteria?

Ralph Common
Dept. of Physiology
Michigan State University


==============================Original Headers==============================
4, 24 -- From rcommon-at-msu.edu Mon Feb 16 14:08:19 2009
4, 24 -- Received: from sys52.mail.msu.edu (sys52.mail.msu.edu [35.9.75.232])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GK8Jlo009180
4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 14:08:19 -0600
4, 24 -- Received: from [35.9.122.125] (helo=emlab)
4, 24 -- by sys52.mail.msu.edu with esmtpsa (Exim 4.63 #12)
4, 24 -- (TLSv1:RC4-MD5:128)
4, 24 -- id 1LZ9lK-0004y0-Tg
4, 24 -- for Microscopy-at-microscopy.com; Mon, 16 Feb 2009 15:08:18 -0500
4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
4, 24 -- To: {Microscopy-at-microscopy.com}
4, 24 -- Subject: TEM of bacteria
4, 24 -- Date: Mon, 16 Feb 2009 15:08:18 -0500
4, 24 -- Message-ID: {001801c99072$4f829840$7d7a0923-at-msu.edu}
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: text/plain;
4, 24 -- charset="iso-8859-1"
4, 24 -- Content-Transfer-Encoding: 7bit
4, 24 -- X-Priority: 3 (Normal)
4, 24 -- X-MSMail-Priority: Normal
4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
4, 24 -- Importance: Normal
4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
4, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Mon, 16 Feb 2009 15:49:23 -0600
Subject: [Microscopy] TEM: diffraction pattern question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,
The SAD is showing you the crystallinity of your sample, where a single,
large selected crystal will give you a regular spot pattern, a large number
of selected crystals will give you sharp rings, like in your gold standard,
and diffuse light with no pattern suggests an amorphous material. You would
get half a ring pattern if your SAD aperture was on the edge of the sample,
the other half of the pattern would be black. You might also get half a
pattern if your aperture was on the thin edge of a thick particle, where the
beam could not penetrate the thicker part. You also might get half a pattern
if your SAD aperture was bridging a crystalline and amorphous area of your
sample or sampling an amorphous oxide layer. Does the pattern change when
you move about the sample? Is the part with no pattern diffuse white or
black? I always go back to the image to see what I am selecting, when I get
a result I don't expect.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: February 16, 2009 11:39 AM
To: maryflet-at-interchange.ubc.ca

Some of our SAD diffraction patterns are showing rings that are
discontinuous. Only a small segment of the rings are sharp (say, a
wedge comprising 25% of the circle) and they taper off to
nothingness, so that directly opposite the sharpest rings, we see
nothing at all. I was wondering what might be causing this (uneven
specimen thickness, specimen tilted, illumination misalignment,
astigmatism). When we diffract a gold standard, no problem, so I
conclude that it must be specimen related.

Any ideas or suggestions to help us solve this SAD situation (sorry,
I couldn't resist....)?
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
4, 17 -- From bozzola-at-siu.edu Mon Feb 16 13:31:51 2009
4, 17 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1GJVpLR012804
4, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:31:51
-0600
4, 17 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu
[131.230.177.136])
4, 17 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id
n1GJVngs024603
4, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:31:50
-0600 (CST)
4, 17 -- Mime-Version: 1.0
4, 17 -- Message-Id: {a06240801c5bf6d5388ee-at-[131.230.177.136]}
4, 17 -- Date: Mon, 16 Feb 2009 13:31:54 -0600
4, 17 -- To: Microscopy-at-microscopy.com
4, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
4, 17 -- Subject: TEM: diffraction pattern question
4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 17 -- X-Spam-Score: 0.00%
4, 17 -- X-MASF: 0.00%
4, 17 -- X-Whitelist: 0.00%
==============================End of - Headers==============================


==============================Original Headers==============================
13, 32 -- From maryflet-at-interchange.ubc.ca Mon Feb 16 15:49:21 2009
13, 32 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15])
13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GLnLaf027334
13, 32 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 15:49:21 -0600
13, 32 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
13, 32 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP id EFCE81F3E3
13, 32 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 13:49:19 -0800 (PST)
13, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
13, 32 -- by smtp.interchange.ubc.ca
13, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
13, 32 -- with ESMTP id {0KF600LRAHY648-at-smtp.interchange.ubc.ca} for
13, 32 -- microscopy-at-microscopy.com; Mon, 16 Feb 2009 13:49:19 -0800 (PST)
13, 32 -- Date: Mon, 16 Feb 2009 13:48:59 -0800
13, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
13, 32 -- Subject: RE: [Microscopy] TEM: diffraction pattern question
13, 32 -- In-reply-to: {200902161939.n1GJdDEp022336-at-ns.microscopy.com}
13, 32 -- To: bozzola-at-siu.edu
13, 32 -- Cc: microscopy-at-microscopy.com
13, 32 -- Reply-to: maryflet-at-interchange.ubc.ca
13, 32 -- Message-id: {0KF600LRBHY648-at-smtp.interchange.ubc.ca}
13, 32 -- Organization: Materials Eng.
13, 32 -- MIME-version: 1.0
13, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
13, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
13, 32 -- Content-type: text/plain; charset=us-ascii
13, 32 -- Content-transfer-encoding: 7bit
13, 32 -- Thread-index: AcmQbj9TCO182JIlTJKLVFidYxNXNwAEKhWw
13, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.2.16.213422
13, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
13, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0
13, 32 -- X-Spam-Level:
13, 32 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: milesd-at-us.ibm.com
Date: Mon, 16 Feb 2009 17:31:38 -0600
Subject: [Microscopy] Re: SEM: Breaking wafers accurately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

You may already be aware of this, but if not...

All of the silicon wafers I work with, and cleave into pieces, have
preferred cleavage planes along the crystal lattice. The directions, and
angles will depend on the type of wafer you have. The wafers I work with,
which are for integrated circuit fabrication, have two very nice cleavage
planes, at 90 degrees apart, which works nicely for cleaving cross
sections of the circuitry. I can cleave a straight line across a 300mm
(12 inch) wafer by making a little scratch, maybe 1/8 inch long, in line
with the cleavage plane, on the edge of the wafer. I then lay the scribe
down on the bench top, with about 1/4 inch of the approximately 1/8 inch
diameter shaft, under the edge of the wafer, lined up with the scratch
mark. Then I hold one side of the wafer down to the bench top, and gently
press the other side down over the scribe shaft. The crack starts at the
scratch, and goes across the wafer. Occasionally, it will pick up a curve
along the way, but most of the time it is as straight as an arrow.

All bets are off, if you have amorphous wafers.

Hope this helped,
Darrell


TindallR-at-missouri.edu wrote on 02/16/2009 10:36:41 AM:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Dear Collective,
}
} This has been the year of challenging projects and the year is still
} young. Now another one has come over the transom.
}
} One of our researchers is experimenting with photo-resist patterns on
} 6-inch silicon wafers. Our task is to image cross-sections of tiny
} little lines contained in rows of tiny little dots about the size of TEM
} grids. Imaging mags are around 100-200kX, so we're talking little here.
} Getting these wafers to break accurately is proving to be a real bear
} and it may be that the break is distorting the cross-sections.
}
} I'm using a diamond scribe to score the wafers as accurately as I can,
} but scribing the back of the wafer to get an accurate break on the front
} is proving to be difficult.
}
} There must be lots of industry folks out there who do this on a daily
} basis. Would someone be willing to share a few tiny little hints?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original
Headers==============================
} 10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009
} 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
} (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n1GFYqGp008710
} 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53
-0600
} 10, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 10, 27 -- X-IronPort-Anti-Spam-Result:
} ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG
} 10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} ([209.106.229.41])
} 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb
} 2009 09:34:50 -0600
} 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.
} 34]) by um-nsmtpout1.um.umsystem.edu with Microsoft
SMTPSVC(6.0.3790.3959);
} 10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600
} 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 10, 27 -- Content-class: urn:content-classes:message
} 10, 27 -- MIME-Version: 1.0
} 10, 27 -- Content-Type: text/plain;
} 10, 27 -- charset="us-ascii"
} 10, 27 -- Subject: SEM: Breaking wafers accurately
} 10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600
} 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM-
} XMAIL08.um.umsystem.edu}
} 10, 27 -- X-MS-Has-Attach:
} 10, 27 -- X-MS-TNEF-Correlator:
} 10, 27 -- Thread-Topic: SEM: Breaking wafers accurately
} 10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww==
} 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 10, 27 -- To: {microscopy-at-microscopy.com}
} 10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC)
} FILETIME=[1B6248B0:01C9904C]
} 10, 27 -- Content-Transfer-Encoding: 8bit
} 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.
} microscopy.com id n1GFYqGp008710
} ==============================End of -
Headers==============================


==============================Original Headers==============================
9, 28 -- From milesd-at-us.ibm.com Mon Feb 16 17:31:37 2009
9, 28 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GNVbRg014117
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 17:31:37 -0600
9, 28 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234])
9, 28 -- by e6.ny.us.ibm.com (8.13.1/8.13.1) with ESMTP id n1GNWMUe005942
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:32:22 -0500
9, 28 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217])
9, 28 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v9.1) with ESMTP id n1GNVa9K187620
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500
9, 28 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1])
9, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id n1GNVanW004499
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500
9, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50])
9, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id n1GNVaNR004491
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500
9, 28 -- In-Reply-To: {200902161536.n1GFafH8009749-at-ns.microscopy.com}
9, 28 -- To: Microscopy-at-Microscopy.Com
9, 28 -- MIME-Version: 1.0
9, 28 -- Subject: Re: [Microscopy] SEM: Breaking wafers accurately
9, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006
9, 28 -- Message-ID: {OF70376F20.871A81D0-ON8525755F.007E7D80-8525755F.0081356C-at-us.ibm.com}
9, 28 -- From: Darrell Miles {milesd-at-us.ibm.com}
9, 28 -- Date: Mon, 16 Feb 2009 18:31:38 -0500
9, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1 HF8|December 19, 2008) at
9, 28 -- 02/16/2009 18:31:40,
9, 28 -- Serialize complete at 02/16/2009 18:31:40
9, 28 -- Content-Type: text/plain; charset="US-ASCII"
==============================End of - Headers==============================




From: leswes-at-shaw.ca
Date: Tue, 17 Feb 2009 09:55:19 -0600
Subject: [Microscopy] Re: SEM: Breaking wafers accurately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On 17-Feb-09, at 7:50 AM, Lesley Weston wrote:

} Ask the researcher, or if necessary the wafer manufacturer, to tell
} you which crystal orientation is used in making the original wafers
} before they are etched, then scribe along a line appropriate to
} that angle. It makes a big difference; scribing and then breaking
} along the wrong angle shatters the wafer. I used to scribe part-way
} on the front of the wafer so that I could see where I was, and the
} break would continue past the score to give nice cross-sections -
} same idea as making glass knives for sectioning.
}
} Lesley Weston.
}
}
} On 16-Feb-09, at 7:39 AM, TindallR-at-missouri.edu wrote:
}
} }
} }
} }
} } ---------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/
} } MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ---------------------------------------------------------------------
} } -------
} }
} } Dear Collective,
} }
} } This has been the year of challenging projects and the year is still
} } young. Now another one has come over the transom.
} }
} } One of our researchers is experimenting with photo-resist patterns on
} } 6-inch silicon wafers. Our task is to image cross-sections of tiny
} } little lines contained in rows of tiny little dots about the size
} } of TEM
} } grids. Imaging mags are around 100-200kX, so we're talking little
} } here.
} } Getting these wafers to break accurately is proving to be a real bear
} } and it may be that the break is distorting the cross-sections.
} }
} } I'm using a diamond scribe to score the wafers as accurately as I
} } can,
} } but scribing the back of the wafer to get an accurate break on the
} } front
} } is proving to be difficult.
} }
} } There must be lots of industry folks out there who do this on a daily
} } basis. Would someone be willing to share a few tiny little hints?
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?
} } Op=Splash&Amount=
} } Week&NavType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }






==============================Original Headers==============================
8, 22 -- From leswes-at-shaw.ca Tue Feb 17 09:55:18 2009
8, 22 -- Received: from idcmail-mo1so.shaw.ca (idcmail-mo1so.shaw.ca [24.71.223.10])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HFtGd4029183
8, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 09:55:17 -0600
8, 22 -- Received: from pd3ml2so-ssvc.prod.shaw.ca ([10.0.141.138])
8, 22 -- by pd4mo1so-svcs.prod.shaw.ca with ESMTP; 17 Feb 2009 08:55:12 -0700
8, 22 -- X-Cloudmark-SP-Filtered: true
8, 22 -- X-Cloudmark-SP-Result: v=1.0 c=0 a=Zx37jsudAAAA:8 a=KKXDUkkEAAAA:8 a=T6abDhuPAAAA:8 a=fMNaZ91lPsFT39mxYYsA:9 a=92rRVBJz9-OWWpaKKowA:7 a=SSRIeVQFFbXejdZ12ejksDrAm-UA:4 a=aDP_jk9KaZEA:10 a=aw0-s5nGupUA:10 a=NROiimMUsE4A:10 a=KEUJ_xxvRqkA:10 a=RaRL5AVhiQEA:10 a=O27iSwohtSwA:10 a=njno5oAFA7sA:10
8, 22 -- Received: from unknown (HELO [96.49.149.2]) ([96.49.149.2])
8, 22 -- by pd3ml2so-dmz.prod.shaw.ca with ESMTP; 17 Feb 2009 08:55:12 -0700
8, 22 -- In-Reply-To: {17F1115D-BE49-4B32-8FB4-AB871E6593C5-at-shaw.ca}
8, 22 -- References: {200902161539.n1GFdnLr012682-at-ns.microscopy.com} {17F1115D-BE49-4B32-8FB4-AB871E6593C5-at-shaw.ca}
8, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1)
8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
8, 22 -- Message-Id: {D3F433C8-4F7D-4F5B-9A2D-4D2BD2BE8A66-at-shaw.ca}
8, 22 -- Cc: Microscopy-at-microscopy.com
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- From: Lesley Weston {leswes-at-shaw.ca}
8, 22 -- Subject: Re: [Microscopy] SEM: Breaking wafers accurately
8, 22 -- Date: Tue, 17 Feb 2009 07:55:05 -0800
8, 22 -- To: Lesley Weston {leswes-at-shaw.ca}
8, 22 -- X-Mailer: Apple Mail (2.753.1)
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Tue, 17 Feb 2009 10:16:17 -0600
Subject: [Microscopy] EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, all.

We want to be able to closely examine the fibers in cross-sections of various types and grades of paper.

The first question we need to answer is what material to embed with, and which type of microtome would be best to use to make the cross-sections? The second question is how important is sputter-coating and does anyone have a recommendation regarding the material to coat with? Lastly, to avoid re-inventing the wheel, does anyone have images of this type that they'd be willing to share?

Robert Zonis
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635
robert.zonis-at-sanford.com
 
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.

This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
7, 31 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 10:16:17 2009
7, 31 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGGEOp003353
7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:16:16 -0600
7, 31 -- X-VirusChecked: Checked
7, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
7, 31 -- X-Msg-Ref: server-10.tower-192.messagelabs.com!1234887369!28438663!2
7, 31 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
7, 31 -- X-Originating-IP: [198.176.16.25]
7, 31 -- Received: (qmail 11161 invoked from network); 17 Feb 2009 16:16:10 -0000
7, 31 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
7, 31 -- by server-10.tower-192.messagelabs.com with RC4-SHA encrypted SMTP; 17 Feb 2009 16:16:10 -0000
7, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
7, 31 -- Tue, 17 Feb 2009 10:16:09 -0600
7, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 31 -- Content-class: urn:content-classes:message
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain;
7, 31 -- charset="iso-8859-1"
7, 31 -- Subject: EM - Cross-sections of paper grades
7, 31 -- Date: Tue, 17 Feb 2009 10:13:30 -0600
7, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401B6C726-at-naoaksasebe02.nr.ad.newellco.com}
7, 31 -- X-MS-Has-Attach:
7, 31 -- X-MS-TNEF-Correlator:
7, 31 -- Thread-Topic: EM - Cross-sections of paper grades
7, 31 -- Thread-Index: AcmRGqSgtbQ6aTrPT5mR+wwnF0kfiQ==
7, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
7, 31 -- To: {Microscopy-at-microscopy.com}
7, 31 -- X-OriginalArrivalTime: 17 Feb 2009 16:16:09.0357 (UTC) FILETIME=[0B2023D0:01C9911B]
7, 31 -- Content-Transfer-Encoding: 8bit
7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HGGEOp003353
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Tue, 17 Feb 2009 10:35:07 -0600
Subject: [Microscopy] RE: TEM: diffraction pattern question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Arced diffraction problem

John,

I think that earlier respondents have missed the point of your query.
From my reading of your question, the answer is that you have a
textured sample. With a polycrystalline sample you get uniform rings
only if the grains are randomly oriented. If the grains have a
preferred orientation then the rings will have intensity that varies
round the diameter of each ring. See Hirsch et al 1965 or 1977
pages116-117; Williams and Carter 1996 pages 273-275 or Reimer 1984
pages 406-407

Good luck,
Alwyn Eades

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
6, 21 -- From jae5-at-lehigh.edu Tue Feb 17 10:35:07 2009
6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZ5vV023318
6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:06 -0600
6, 21 -- Received: from [127.0.0.1] (r054054.mat.Lehigh.EDU [128.180.54.54])
6, 21 -- (authenticated bits=0)
6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n1HGYZlb031426
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:35:03 -0500
6, 21 -- Message-ID: {499AE71F.8040104-at-lehigh.edu}
6, 21 -- Date: Tue, 17 Feb 2009 11:34:39 -0500
6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
6, 21 -- Organization: Lehigh University
6, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 21 -- MIME-Version: 1.0
6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
6, 21 -- Subject: RE: TEM: diffraction pattern question
6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu
6, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: tpepper-at-iastate.edu
Date: Tue, 17 Feb 2009 10:35:22 -0600
Subject: [Microscopy] A challenge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I love being a biological service facility! I have a student from electrical
engineering that has 'polymer' fibers that are water soluble and sensitive to
temperatures below freezing. He wants TEM cross-sections of the fibers. 0_o

The gauntlet has been thrown, any ideas? Cryo's out due to temp issue, so can
one section dry at room temp (provided the fibers don't dissolve in any resin)?
Sorry I don't know the constitution of the fibers, just the few physical
issues with them-they apper to be thinner than, but similar in appearence to,
spiderweb...
(I love challenges!)

Thanks in advance!

Tracey Pepper
Microscopy and NanoImaging Facility
Iowa State University
Ames, IA 50011-1020
(515) 294-3872



==============================Original Headers==============================
6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009
6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZKEj023698
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:20 -0600
6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n1HGZI0S017865
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:18 -0600
6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6;
6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600
6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32])
6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id n1HGZGrc024171
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:16 -0600
6, 21 -- To: microscopy-at-microscopy.com
6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu}
6, 21 -- Subject: A challenge
6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST)
6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU Version mp9.11
6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu}
6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.17.162227
6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0, FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __SANE_MSGID 0'
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Tue, 17 Feb 2009 10:48:45 -0600
Subject: [Microscopy] Re: EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

Both JEOL and Gatan show cross sections of paper in some of the
product ads. Check their websites for info.


Roseann Csencsits, PhD
Scientist in Charge - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548







On Feb 17, 2009, at 8:30 AM, Robert.Zonis-at-Sanford.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi, all.
}
} We want to be able to closely examine the fibers in cross-sections
} of various types and grades of paper.
}
} The first question we need to answer is what material to embed with,
} and which type of microtome would be best to use to make the cross-
} sections? The second question is how important is sputter-coating
} and does anyone have a recommendation regarding the material to coat
} with? Lastly, to avoid re-inventing the wheel, does anyone have
} images of this type that they'd be willing to share?
}
} Robert Zonis
} Sanford L.P. - A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
} robert.zonis-at-sanford.com
}
} This message is intended for the Microscopy Listserv. Permission is
} specifically granted to the Microscopy Society of America to publish
} some or all of this message in the Microscopy Today journal.
}
} This message may contain information that is confidential and/or
} protected by law. If the reader of this message is not the intended
} recipient, you are hereby notified that any dissemination,
} distribution, copying or communication of this message is strictly
} prohibited. If you have received this communication in error, please
} contact the sender immediately and delete the message. Please note
} that although we will take all commercially reasonable efforts to
} prevent viruses from being transmitted from our systems, it is the
} responsibility of the recipient to check for and prevent adverse
} action by viruses on its own systems.
}
} ______________________________________________________________________
} This email has been scanned by the MessageLabs Email Security System.
} For more information please visit http://www.messagelabs.com/email
} ______________________________________________________________________
}
}
} ==============================Original
} Headers==============================
} 7, 31 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 10:16:17 2009
} 7, 31 -- Received: from mail192.messagelabs.com
} (mail192.messagelabs.com [216.82.241.243])
} 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n1HGGEOp003353
} 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:16:16
} -0600
} 7, 31 -- X-VirusChecked: Checked
} 7, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
} 7, 31 -- X-Msg-Ref: server-10.tower-192.messagelabs.com!1234887369!
} 28438663!2
} 7, 31 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
} 7, 31 -- X-Originating-IP: [198.176.16.25]
} 7, 31 -- Received: (qmail 11161 invoked from network); 17 Feb 2009
} 16:16:10 -0000
} 7, 31 -- Received: from naehub2.newellco.com (HELO
} naehub2.newellco.com) (198.176.16.25)
} 7, 31 -- by server-10.tower-192.messagelabs.com with RC4-SHA
} encrypted SMTP; 17 Feb 2009 16:16:10 -0000
} 7, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com
} ([10.217.158.64]) by naehub2.newellco.com with Microsoft
} SMTPSVC(6.0.3790.1830);
} 7, 31 -- Tue, 17 Feb 2009 10:16:09 -0600
} 7, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 7, 31 -- Content-class: urn:content-classes:message
} 7, 31 -- MIME-Version: 1.0
} 7, 31 -- Content-Type: text/plain;
} 7, 31 -- charset="iso-8859-1"
} 7, 31 -- Subject: EM - Cross-sections of paper grades
} 7, 31 -- Date: Tue, 17 Feb 2009 10:13:30 -0600
} 7, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401B6C726-at-naoaksasebe02.nr.ad.newellco.com
} }
} 7, 31 -- X-MS-Has-Attach:
} 7, 31 -- X-MS-TNEF-Correlator:
} 7, 31 -- Thread-Topic: EM - Cross-sections of paper grades
} 7, 31 -- Thread-Index: AcmRGqSgtbQ6aTrPT5mR+wwnF0kfiQ==
} 7, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
} 7, 31 -- To: {Microscopy-at-microscopy.com}
} 7, 31 -- X-OriginalArrivalTime: 17 Feb 2009 16:16:09.0357 (UTC)
} FILETIME=[0B2023D0:01C9911B]
} 7, 31 -- Content-Transfer-Encoding: 8bit
} 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n1HGGEOp003353
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
13, 26 -- From RCsencsits-at-lbl.gov Tue Feb 17 10:48:44 2009
13, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGmg21021076
13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:48:43 -0600
13, 26 -- X-Ironport-SBRS: 2.3
13, 26 -- X-IronPort-Anti-Spam-Filtered: true
13, 26 -- X-IronPort-Anti-Spam-Result: AoEGAIx5mkmAAykYe2dsb2JhbACBSZMAAQEWIgVbrksJjw+CXoE1Bg
13, 26 -- X-IronPort-AV: E=Sophos;i="4.38,224,1233561600";
13, 26 -- d="scan'208";a="117160986"
13, 26 -- Received: from mta1.lbl.gov ([128.3.41.24])
13, 26 -- by ironport1.lbl.gov with ESMTP; 17 Feb 2009 08:48:32 -0800
13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246])
13, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n1HGmVpn013486;
13, 26 -- Tue, 17 Feb 2009 08:48:31 -0800 (PST)
13, 26 -- Cc: Microscopy-at-microscopy.com
13, 26 -- Message-Id: {A5B7D447-1D60-4069-A996-FC53803C7890-at-lbl.gov}
13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
13, 26 -- To: Robert.Zonis-at-Sanford.com
13, 26 -- In-Reply-To: {200902171630.n1HGURrM018899-at-ns.microscopy.com}
13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 26 -- Content-Transfer-Encoding: 7bit
13, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 26 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades
13, 26 -- Date: Tue, 17 Feb 2009 08:48:28 -0800
13, 26 -- References: {200902171630.n1HGURrM018899-at-ns.microscopy.com}
13, 26 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 17 Feb 2009 10:58:54 -0600
Subject: [Microscopy] A challenge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wow, that's a tough one. Ranks right up there with our guy who wants to
see the size of water droplets in diesel fuel.

Anyway, I guess the first thing I'd try is to test the fibers in some
resins to see if they stay together. If they don't dissolve, I'd try
embedding them and dry-cutting rather large sections with a glass knife,
then using a clamshell (hinged) grid to pop them into the TEM without
the need to adhere them to anything.

Good luck!

Cheers,
Randy

Electron Microscopy Core Staff
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304 / 4777
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan

Note: This mailing is intended for past and present users of the
Electron Microscopy Core Facility and for those with an interest in
electron microscopy. Please let us know if you receive more than one
copy of these announcements. If you would like to be removed from this
list, please reply to this message and request to be unsubscribed. Thank
you.




-----Original Message-----
X-from: tpepper-at-iastate.edu [mailto:tpepper-at-iastate.edu]
Sent: Tuesday, February 17, 2009 10:37 AM
To: Tindall, Randy D.

Hello,
I love being a biological service facility! I have a student from
electrical
engineering that has 'polymer' fibers that are water soluble and
sensitive to
temperatures below freezing. He wants TEM cross-sections of the fibers.
0_o

The gauntlet has been thrown, any ideas? Cryo's out due to temp issue,
so can
one section dry at room temp (provided the fibers don't dissolve in any
resin)?
Sorry I don't know the constitution of the fibers, just the few physical

issues with them-they apper to be thinner than, but similar in
appearence to,
spiderweb...
(I love challenges!)

Thanks in advance!

Tracey Pepper
Microscopy and NanoImaging Facility
Iowa State University
Ames, IA 50011-1020
(515) 294-3872



==============================Original
Headers==============================
6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009
6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu
[129.186.140.14])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n1HGZKEj023698
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:20 -0600
6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu
[129.186.1.48])
6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with
SMTP id n1HGZI0S017865
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:18 -0600
6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by
devirus-11.iastate.edu with smtp
6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6;
6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600
6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32])
6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id
n1HGZGrc024171
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:16 -0600
6, 21 -- To: microscopy-at-microscopy.com
6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu}
6, 21 -- Subject: A challenge
6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST)
6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU
Version mp9.11
6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu}
6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393,
Antispam-Data: 2009.2.17.162227
6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0,
BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0,
FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0,
__MIME_TEXT_ONLY 0, __SANE_MSGID 0'
==============================End of -
Headers==============================


==============================Original Headers==============================
21, 30 -- From TindallR-at-missouri.edu Tue Feb 17 10:58:54 2009
21, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGwqQ3002803
21, 30 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:58:53 -0600
21, 30 -- X-IronPort-Anti-Spam-Filtered: true
21, 30 -- X-IronPort-Anti-Spam-Result: ApoEABt8mknRauUo/2dsb2JhbADCToE+AQmGRohKglIMgTUGhhA
21, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
21, 30 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 17 Feb 2009 10:58:51 -0600
21, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
21, 30 -- Tue, 17 Feb 2009 10:58:51 -0600
21, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 30 -- Content-class: urn:content-classes:message
21, 30 -- MIME-Version: 1.0
21, 30 -- Content-Type: text/plain;
21, 30 -- charset="us-ascii"
21, 30 -- Subject: RE: [Microscopy] A challenge
21, 30 -- Date: Tue, 17 Feb 2009 10:58:50 -0600
21, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7E11-at-UM-XMAIL08.um.umsystem.edu}
21, 30 -- In-Reply-To: {200902171637.n1HGb41P027702-at-ns.microscopy.com}
21, 30 -- X-MS-Has-Attach:
21, 30 -- X-MS-TNEF-Correlator:
21, 30 -- Thread-Topic: [Microscopy] A challenge
21, 30 -- Thread-Index: AcmRHfi8z7B9/rL5Tm+yTCNGPLmEKAAAqLfA
21, 30 -- References: {200902171637.n1HGb41P027702-at-ns.microscopy.com}
21, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
21, 30 -- To: {tpepper-at-iastate.edu}
21, 30 -- Cc: {microscopy-at-microscopy.com}
21, 30 -- X-OriginalArrivalTime: 17 Feb 2009 16:58:51.0223 (UTC) FILETIME=[021DDE70:01C99121]
21, 30 -- Content-Transfer-Encoding: 8bit
21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HGwqQ3002803
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 17 Feb 2009 11:14:43 -0600
Subject: [Microscopy] Re: EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What sort of EM do you want to do? Your first
question is about embedding for ultramicrotomy
and transmission EM, your second question is
about coating for scanning EM.
If the latter, don't embed, just cryofracture the
paper in liquid nitrogen, mount on edge (support
the paper with silver-paint coated pieces of
applicator stick or the like), or on a stub with
a tilted surface, and sputter-coat with gold or
better, gold-palladium. If you have a low-voltage
or environmental/low-vacuum SEM, you may not need
to coat. Are the fibers wood (or other plant) or
polymer or ... ?
This works for both coated and uncoated papers.
Just be sure the samples are dry.

Phil

} Hi, all.
}
} We want to be able to closely examine the fibers
} in cross-sections of various types and grades of
} paper.
}
} The first question we need to answer is what
} material to embed with, and which type of
} microtome would be best to use to make the
} cross-sections? The second question is how
} important is sputter-coating and does anyone
} have a recommendation regarding the material to
} coat with? Lastly, to avoid re-inventing the
} wheel, does anyone have images of this type that
} they'd be willing to share?
}
} Robert Zonis
} Sanford L.P.Ý- A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
} robert.zonis-at-sanford.com
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009
4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHEftn017299
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:14:42 -0600
4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n1HHEaiF017173
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:14:38 -0500
4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500
4, 27 -- Mime-Version: 1.0
4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]}
4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500
4, 27 -- To: Microscopy-at-microscopy.com
4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades
4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC) FILETIME=[253F5440:01C99123]
4, 27 -- X-Canit-CHI2: 0.00
4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
4, 27 -- X-CanItPRO-Stream: default
4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df
4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
4, 27 -- Content-Transfer-Encoding: 8bit
4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHEftn017299
==============================End of - Headers==============================




From: ZZhang-at-uwyo.edu
Date: Tue, 17 Feb 2009 11:27:11 -0600
Subject: [Microscopy] A challenge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tracey:

I am a biologist too. I did help a user from Chemical Engineering to
section a polymer. I simply throw the polymer (pre-stained with Osmium)
into the Spurr resin, let it be "infiltrated" for a couple of hours, and
put it in the oven to polymerize. The sections turned pretty well.

Give it a try, it might work.

Zhaojie Zhang
Director, Microscopy Core Facility
University of Wyoming
Laramie, WY 82071
zzhng-at-uwyo.edu


-----Original Message-----
X-from: tpepper-at-iastate.edu [mailto:tpepper-at-iastate.edu]
Sent: Tuesday, February 17, 2009 9:41 AM
To: Z.J. Zhang

Hello,
I love being a biological service facility! I have a student from
electrical
engineering that has 'polymer' fibers that are water soluble and
sensitive to
temperatures below freezing. He wants TEM cross-sections of the fibers.
0_o

The gauntlet has been thrown, any ideas? Cryo's out due to temp issue,
so can
one section dry at room temp (provided the fibers don't dissolve in any
resin)?
Sorry I don't know the constitution of the fibers, just the few physical

issues with them-they apper to be thinner than, but similar in
appearence to,
spiderweb...
(I love challenges!)

Thanks in advance!

Tracey Pepper
Microscopy and NanoImaging Facility
Iowa State University
Ames, IA 50011-1020
(515) 294-3872



==============================Original
Headers==============================
6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009
6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu
[129.186.140.14])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n1HGZKEj023698
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:20 -0600
6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu
[129.186.1.48])
6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with
SMTP id n1HGZI0S017865
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:18 -0600
6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by
devirus-11.iastate.edu with smtp
6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6;
6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600
6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32])
6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id
n1HGZGrc024171
6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
10:35:16 -0600
6, 21 -- To: microscopy-at-microscopy.com
6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu}
6, 21 -- Subject: A challenge
6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST)
6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU
Version mp9.11
6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu}
6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393,
Antispam-Data: 2009.2.17.162227
6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0,
BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0,
FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0,
__MIME_TEXT_ONLY 0, __SANE_MSGID 0'
==============================End of -
Headers==============================


==============================Original Headers==============================
18, 32 -- From ZZhang-at-uwyo.edu Tue Feb 17 11:27:10 2009
18, 32 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHR9JQ031609
18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:27:10 -0600
18, 32 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208])
18, 32 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id n1HHOOXN017103;
18, 32 -- Tue, 17 Feb 2009 10:27:03 -0700 (MST)
18, 32 -- (envelope-from ZZhang-at-uwyo.edu)
18, 32 -- Received: from TELEGRAPH1.uwyo.edu (10.84.60.121) by ponyexpress-ht1.uwyo.edu
18, 32 -- (10.84.60.208) with Microsoft SMTP Server id 8.1.340.0; Tue, 17 Feb 2009
18, 32 -- 10:26:50 -0700
18, 32 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by TELEGRAPH1.uwyo.edu
18, 32 -- with Microsoft SMTPSVC(6.0.3790.3959); Tue, 17 Feb 2009 10:26:49 -0700
18, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
18, 32 -- Content-Class: urn:content-classes:message
18, 32 -- MIME-Version: 1.0
18, 32 -- Content-Type: text/plain; charset="US-ASCII"
18, 32 -- Subject: RE: [Microscopy] A challenge
18, 32 -- Date: Tue, 17 Feb 2009 10:26:48 -0700
18, 32 -- Message-ID: {D0162B092A0F46429960E910DE8B3C4902F5476D-at-TELEGRAPH5.uwyo.edu}
18, 32 -- In-Reply-To: {200902171641.n1HGf14x006121-at-ns.microscopy.com}
18, 32 -- X-MS-Has-Attach:
18, 32 -- X-MS-TNEF-Correlator:
18, 32 -- Thread-Topic: [Microscopy] A challenge
18, 32 -- Thread-Index: AcmRHolWhQE/Vu6/SgSXWBf4/AN3DQABKkhw
18, 32 -- References: {200902171641.n1HGf14x006121-at-ns.microscopy.com}
18, 32 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
18, 32 -- To: {microscopy-at-microscopy.com}
18, 32 -- CC: {tpepper-at-iastate.edu}
18, 32 -- X-OriginalArrivalTime: 17 Feb 2009 17:26:49.0595 (UTC) FILETIME=[EA811CB0:01C99124]
18, 32 -- Content-Transfer-Encoding: 8bit
18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHR9JQ031609
==============================End of - Headers==============================




From: Jacqueline.Ayotte-at-ticona.com
Date: Tue, 17 Feb 2009 11:45:56 -0600
Subject: [Microscopy] EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Robert,

You want to view a cross section of the papers, but do not necessarily need to create a thin section?
If so, mount the papers in epoxy and polish to achieve a cross section.
You may not need to coat if you have an SEM capable of low voltage and/or low vacuum.

What is your end objective - that would help in determining the best sample prep.

I have examined cross sections of paper before using embedding and polishing, however it might not be suitable for your purposes.

We can discuss more off-line if you wish and share images.

Jacqueline

Jacqueline Ayotte
Microscopist - Advanced Materials Characterization
Ticona
8040 Dixie Highway
} Florence KY 41042
} 859-372-3139
} fax 859-372-3184
} jacqueline.ayotte-at-ticona.com
The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver

What sort of EM do you want to do? Your first question is about embedding for ultramicrotomy and transmission EM, your second question is about coating for scanning EM.
If the latter, don't embed, just cryofracture the paper in liquid nitrogen, mount on edge (support the paper with silver-paint coated pieces of applicator stick or the like), or on a stub with a tilted surface, and sputter-coat with gold or better, gold-palladium. If you have a low-voltage or environmental/low-vacuum SEM, you may not need to coat. Are the fibers wood (or other plant) or polymer or ... ?
This works for both coated and uncoated papers.
Just be sure the samples are dry.

Phil

} Hi, all.
}
} We want to be able to closely examine the fibers in cross-sections of
} various types and grades of paper.
}
} The first question we need to answer is what material to embed with,
} and which type of microtome would be best to use to make the
} cross-sections? The second question is how important is sputter-coating
} and does anyone have a recommendation regarding the material to coat
} with? Lastly, to avoid re-inventing the wheel, does anyone have images
} of this type that they'd be willing to share?
}
} Robert Zonis
} Sanford L.P.Ý- A Newell Rubbermaid Company Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
} robert.zonis-at-sanford.com
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009 4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHEftn017299
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:14:42 -0600
4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n1HHEaiF017173
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:14:38 -0500
4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500
4, 27 -- Mime-Version: 1.0
4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]}
4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC) FILETIME=[253F5440:01C99123] 4, 27 -- X-Canit-CHI2: 0.00 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
4, 27 -- X-CanItPRO-Stream: default
4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 4, 27 -- Content-Transfer-Encoding: 8bit 4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHEftn017299 ==============================End of - Headers==============================


==============================Original Headers==============================
16, 31 -- From Jacqueline.Ayotte-at-ticona.com Tue Feb 17 11:45:34 2009
16, 31 -- Received: from exprod7og106.obsmtp.com (exprod7og106.obsmtp.com [64.18.2.165])
16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1HHjUsu013517
16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:45:32 -0600
16, 31 -- Received: from source ([148.163.79.14]) by exprod7ob106.postini.com ([64.18.6.12]) with SMTP
16, 31 -- ID DSNKSZr3sTgJhZC9gGIrSbv0Ov60aofupNoK-at-postini.com; Tue, 17 Feb 2009 09:45:33 PST
16, 31 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959);
16, 31 -- Tue, 17 Feb 2009 11:52:48 -0600
16, 31 -- Received: from amalnxmbx1.SW.CZDS.BZ ([148.163.82.91]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
16, 31 -- Tue, 17 Feb 2009 11:45:13 -0600
16, 31 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxmbx1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
16, 31 -- Tue, 17 Feb 2009 11:45:12 -0600
16, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 31 -- Content-class: urn:content-classes:message
16, 31 -- MIME-Version: 1.0
16, 31 -- Content-Type: text/plain;
16, 31 -- charset="iso-8859-1"
16, 31 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades
16, 31 -- Date: Tue, 17 Feb 2009 12:45:10 -0500
16, 31 -- Message-ID: {A13B1B747CE9A748BE639EB5A1ACE126013D7229-at-amfloxmbx1.SW.CZDS.BZ}
16, 31 -- In-Reply-To: {200902171724.n1HHOWK5031290-at-ns.microscopy.com}
16, 31 -- X-MS-Has-Attach:
16, 31 -- X-MS-TNEF-Correlator:
16, 31 -- Thread-Topic: [Microscopy] Re: EM - Cross-sections of paper grades
16, 31 -- Thread-Index: AcmRJJxAYhx2j44lR7y2CBw5iJwSgAAAWU2w
16, 31 -- References: {200902171724.n1HHOWK5031290-at-ns.microscopy.com}
16, 31 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com}
16, 31 -- To: {Microscopy-at-microscopy.com}
16, 31 -- X-OriginalArrivalTime: 17 Feb 2009 17:45:12.0928 (UTC) FILETIME=[7C245E00:01C99127]
16, 31 -- Content-Transfer-Encoding: 8bit
16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHjUsu013517
==============================End of - Headers==============================




From: tom-at-TomKaye.com
Date: Tue, 17 Feb 2009 11:52:01 -0600
Subject: [Microscopy] Re: EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ah! Great idea, thanks! I had problems embedding dollar bills.

I love this list., no bickering no fighting, just good info.

Tom Kaye



-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Tuesday, February 17, 2009 10:20 AM
To: tom-at-tomkaye.com

What sort of EM do you want to do? Your first
question is about embedding for ultramicrotomy
and transmission EM, your second question is
about coating for scanning EM.
If the latter, don't embed, just cryofracture the
paper in liquid nitrogen, mount on edge (support
the paper with silver-paint coated pieces of
applicator stick or the like), or on a stub with
a tilted surface, and sputter-coat with gold or
better, gold-palladium. If you have a low-voltage
or environmental/low-vacuum SEM, you may not need
to coat. Are the fibers wood (or other plant) or
polymer or ... ?
This works for both coated and uncoated papers.
Just be sure the samples are dry.

Phil

} Hi, all.
}
} We want to be able to closely examine the fibers
} in cross-sections of various types and grades of
} paper.
}
} The first question we need to answer is what
} material to embed with, and which type of
} microtome would be best to use to make the
} cross-sections? The second question is how
} important is sputter-coating and does anyone
} have a recommendation regarding the material to
} coat with? Lastly, to avoid re-inventing the
} wheel, does anyone have images of this type that
} they'd be willing to share?
}
} Robert Zonis
} Sanford L.P.Ý- A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
} robert.zonis-at-sanford.com
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009
4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1HHEftn017299
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
11:14:42 -0600
4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id
n1HHEaiF017173
4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009
12:14:38 -0500
4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by
egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500
4, 27 -- Mime-Version: 1.0
4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]}
4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com}
4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500
4, 27 -- To: Microscopy-at-microscopy.com
4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades
4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC)
FILETIME=[253F5440:01C99123]
4, 27 -- X-Canit-CHI2: 0.00
4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00]
L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
4, 27 -- X-CanItPRO-Stream: default
4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df
4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
4, 27 -- Content-Transfer-Encoding: 8bit
4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1HHEftn017299
==============================End of - Headers==============================


==============================Original Headers==============================
15, 22 -- From tom-at-TomKaye.com Tue Feb 17 11:52:01 2009
15, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com [66.102.127.13])
15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHq0Jo021955
15, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:52:00 -0600
15, 22 -- Received: from TKdual ([67.134.109.68]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959);
15, 22 -- Tue, 17 Feb 2009 11:51:58 -0600
15, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com}
15, 22 -- To: {microscopy-at-microscopy.com}
15, 22 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades
15, 22 -- Date: Tue, 17 Feb 2009 10:53:53 -0700
15, 22 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGOEFIADAB.tom-at-tomkaye.com}
15, 22 -- MIME-Version: 1.0
15, 22 -- Content-Type: text/plain;
15, 22 -- charset="iso-8859-1"
15, 22 -- Content-Transfer-Encoding: 8bit
15, 22 -- X-Priority: 3 (Normal)
15, 22 -- X-MSMail-Priority: Normal
15, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0)
15, 22 -- Importance: Normal
15, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
15, 22 -- In-Reply-To: {200902171720.n1HHKEKo023615-at-ns.microscopy.com}
15, 22 -- X-OriginalArrivalTime: 17 Feb 2009 17:51:58.0364 (UTC) FILETIME=[6DCCFDC0:01C99128]
==============================End of - Headers==============================




From: smalinskas-at-yahoo.com
Date: Tue, 17 Feb 2009 13:00:00 -0600
Subject: [Microscopy] Re: EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That helps a lot, Phil. I couldn't figure out how to get a clean
cross-section without a microtome, so I asked about TEM techniques. As a
novice at TEM and sputter coating, I was hoping that I'd be able to run
both scanning and transmission EM with samples from the same block of
embedded paper sample - it sounds like that's not possible.

We do have a low-vacuum SEM on-site here, but I just assumed I'd have to
do most or all of this study with a STEM at our local university. If
that liquid nitrogen technique gives me good edges, I'll be able to do
this here.

We want to look at standard, ordinary wood-fiber paper, with and without
various types of ink written on it, hopefully being able to see how the
inks interact with and penetrate into the paper fibers and sizing
agents.

Robert Zonis

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Tuesday, February 17, 2009 11:22 AM
To: Zonis, Robert

Robert,

I remember JEOL recently sending me a newsletter on this subject. If you go to their web site, www.jeolusa.com, you can get information on cross-sectioning paper for SEM examination. They also have images of paper cross sections you can look at. On their site, navigate by clicking on Products/Sample Preparation Equipment/Cross Section Polisher. On that page you can click on their Image Gallery. Naturally, this is all centered around the Cross Section Polisher that they are marketing.

Disclaimer: I have no financial interests in JEOL. I'm only a satisfied user of their products.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan


--- On Tue, 2/17/09, Robert.Zonis-at-Sanford.com {Robert.Zonis-at-Sanford.com} wrote:
}
} Hi, all.
}
} We want to be able to closely examine the fibers in
} cross-sections of various types and grades of paper.
}
} The first question we need to answer is what material to
} embed with, and which type of microtome would be best to use
} to make the cross-sections? The second question is how
} important is sputter-coating and does anyone have a
} recommendation regarding the material to coat with? Lastly,
} to avoid re-inventing the wheel, does anyone have images of
} this type that they'd be willing to share?
}
} Robert Zonis
} Sanford L.P. - A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
} robert.zonis-at-sanford.com
}  





==============================Original Headers==============================
9, 23 -- From smalinskas-at-yahoo.com Tue Feb 17 13:00:00 2009
9, 23 -- Received: from web34404.mail.mud.yahoo.com (web34404.mail.mud.yahoo.com [66.163.178.153])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1HIxvWL025101
9, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 17 Feb 2009 12:59:59 -0600
9, 23 -- Received: (qmail 62770 invoked by uid 60001); 17 Feb 2009 18:59:55 -0000
9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
9, 23 -- s=s1024; d=yahoo.com;
9, 23 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
9, 23 -- b=163EFy8gZwjqHzNn6SvhZJ7w87kcHF21wd6FtLBYOP/BA1r8dhA0C7+CCCnpTLMnuuO9kcOcXjXaKFXsmAEMl2jw/lgR0cjEBII1jF/fpgu2WvoBK0EYDnZgmaA9nmFoAcDhDqpQZ/n3h147ayGZgESLeCTi38520e2DN5iUYrs=;
9, 23 -- X-YMail-OSG: bCGw8VwVM1klOXHiR5GrghrkcQixLyzkzjmtMeiEYIBFVhAruL8zejea8C5iIgh7q9SxlVc91XcwzNsMGBeMsq_GXe_5Vspc5RNFNQN5r6dDMD5.78j1wz1Ujzwfcur19RopWG0dI.0gQOutoLpfB4UPl1tlBpFdUdZZ7F8dN0ed5lIO.vGPjM1wStElFzrds6xc7QjDjMeA_lDyXjOWH1e_jqldngQ-
9, 23 -- Received: from [141.151.33.213] by web34404.mail.mud.yahoo.com via HTTP; Tue, 17 Feb 2009 10:59:55 PST
9, 23 -- X-Mailer: YahooMailWebService/0.7.260.1
9, 23 -- Date: Tue, 17 Feb 2009 10:59:55 -0800 (PST)
9, 23 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 23 -- Reply-To: smalinskas-at-yahoo.com
9, 23 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades
9, 23 -- To: Robert.Zonis-at-Sanford.com, microscopy-at-ns.microscopy.com
9, 23 -- In-Reply-To: {200902171618.n1HGIibW006216-at-ns.microscopy.com}
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain; charset=iso-8859-1
9, 23 -- Message-ID: {305999.62206.qm-at-web34404.mail.mud.yahoo.com}
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HIxvWL025101
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Tue, 17 Feb 2009 13:29:07 -0600
Subject: [Microscopy] Post-Doc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This posting is from one of my users. Please reply directly to Dr.
Hailiang Dong's contact information below.

====

Geology: Postdoctoral Fellow (position is pending funding) to perform
mineralogical characterizations using X-ray diffraction, scanning and
transmission electron microscopy (SEM and TEM), and mineral-microbe
interactions; install and set up new TEM; work in a team in the field
including site characterization and core collection as well as
numerical modeling.

Require: Ph.D in mineralogy or materials science; extensive
experience in TEM, electron energy loss spectroscopy (EELS),
mineral-microbe interactions (microbial reduction and oxidation of
metals), and wet chemistry; good oral and written communication
skills.

Desire: Experience in synchrotron-based techniques, Mossbauer
spectroscopy, molecular microbiology, microbial ecology, and/or
metagenomics and microarrays. This is a one-year appointment with
re-appointment subject to continued funding and satisfactory
performance.

Send letter of application, curriculum vitae, and names and contact
information for three references to:

Dr. Hailiang Dong
Department of Geology
Miami University
Oxford, OH 45056.

Contact phone number is 513-529-2517
Email is dongh-at-muohio.edu.

Review of applications will begin on April 1, 2009 and will continue
until the position is filled.

Miami University is an affirmative action/equal opportunity employer
with smoke-free campuses. For information regarding campus crime and
safety, visit www.muohio.edu/righttoknow.
Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


==============================Original Headers==============================
12, 22 -- From edelmare-at-muohio.edu Tue Feb 17 13:29:05 2009
12, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HJT4rB007605
12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 13:29:04 -0600
12, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11])
12, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n1HJT6fE019007
12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 14:29:06 -0500
12, 22 -- Received: from [192.168.1.23] ([134.53.14.105])
12, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n1HJT3NO022524
12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 14:29:03 -0500
12, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
12, 22 -- To: microscopy-at-Microscopy.com
12, 22 -- Date: Tue, 17 Feb 2009 14:29:03 -0500
12, 22 -- MIME-Version: 1.0
12, 22 -- Subject: Post-Doc Position
12, 22 -- Message-ID: {499AC9AF.17804.68FBC15-at-edelmare.muohio.edu}
12, 22 -- Priority: normal
12, 22 -- X-mailer: Pegasus Mail for Windows (4.41)
12, 22 -- Content-type: text/plain; charset=US-ASCII
12, 22 -- Content-transfer-encoding: 7BIT
12, 22 -- Content-description: Mail message body
12, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Tue, 17 Feb 2009 15:40:06 -0600
Subject: [Microscopy] Re: EM - Cross-sections of paper grades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

A group across the road at the ANU were looking at ink penetration into
paper, I read about it in one of their monthly research blurbs. I guess the
list doesn't accept attachments, but you can check out their stuff at
http://www.anu.edu.au/CSEM/newsletters/2003/Nov03.pdf and there's a bit more
here:
http://www.anu.edu.au/CSEM/newsletters/2005/MMMar05.pdf

The upshot was essentially as suggested by Phil - snap-freeze at different
stages of ink penetration and observe, still frozen, by SEM - the snag is
that it does require a cryostage in the microscope, though if you have the
sample attached to a big enough lump of metal that's very cold, the sample
will stay cold for quite a while.

Aha, I found a reference: Roberts R, Senden T, Knackstedt M, Lyne MB (2003)
Spreading of aqueous liquids in unsized papers is by film flow. Journal of
Pulp and Paper Science 29: 123-131

May be a bit off track, but I remember they had a lot of fun with this...

cheers,
Roseamry

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 18/02/09 5:13 AM, "Robert.Zonis-at-Sanford.com" {Robert.Zonis-at-Sanford.com}
wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} That helps a lot, Phil. I couldn't figure out how to get a clean
} cross-section without a microtome, so I asked about TEM techniques. As a
} novice at TEM and sputter coating, I was hoping that I'd be able to run
} both scanning and transmission EM with samples from the same block of
} embedded paper sample - it sounds like that's not possible.
}
} We do have a low-vacuum SEM on-site here, but I just assumed I'd have to
} do most or all of this study with a STEM at our local university. If
} that liquid nitrogen technique gives me good edges, I'll be able to do
} this here.
}
} We want to look at standard, ordinary wood-fiber paper, with and without
} various types of ink written on it, hopefully being able to see how the
} inks interact with and penetrate into the paper fibers and sizing
} agents.
}
} Robert Zonis
}
} This message is intended for the Microscopy Listserv. Permission is
} specifically granted to the Microscopy Society of America to publish
} some or all of this message in the Microscopy Today journal.
}
}
} -----Original Message-----
} X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
} Sent: Tuesday, February 17, 2009 11:22 AM
} To: Zonis, Robert
} Subject: [Microscopy] Re: EM - Cross-sections of paper grades
}
}
} What sort of EM do you want to do? Your first
} question is about embedding for ultramicrotomy
} and transmission EM, your second question is
} about coating for scanning EM.
} If the latter, don't embed, just cryofracture the
} paper in liquid nitrogen, mount on edge (support
} the paper with silver-paint coated pieces of
} applicator stick or the like), or on a stub with
} a tilted surface, and sputter-coat with gold or
} better, gold-palladium. If you have a low-voltage
} or environmental/low-vacuum SEM, you may not need
} to coat. Are the fibers wood (or other plant) or
} polymer or ... ?
} This works for both coated and uncoated papers.
} Just be sure the samples are dry.
}
} Phil
}
}
} This message may contain information that is confidential and/or protected by
} law. If the reader of this message is not the intended recipient, you are
} hereby notified that any dissemination, distribution, copying or communication
} of this message is strictly prohibited. If you have received this
} communication in error, please contact the sender immediately and delete the
} message. Please note that although we will take all commercially reasonable
} efforts to prevent viruses from being transmitted from our systems, it is the
} responsibility of the recipient to check for and prevent adverse action by
} viruses on its own systems.
}
} ______________________________________________________________________
} This email has been scanned by the MessageLabs Email Security System.
} For more information please visit http://www.messagelabs.com/email
} ______________________________________________________________________
}
}
} ==============================Original Headers==============================
} 14, 34 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 12:06:25 2009
} 14, 34 -- Received: from mail96.messagelabs.com (mail96.messagelabs.com
} [216.82.254.19])
} 14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n1HI6NIO009186
} 14, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:06:24 -0600
} 14, 34 -- X-VirusChecked: Checked
} 14, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
} 14, 34 -- X-Msg-Ref: server-2.tower-96.messagelabs.com!1234893978!55739307!3
} 14, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
} 14, 34 -- X-Originating-IP: [198.176.16.26]
} 14, 34 -- Received: (qmail 19039 invoked from network); 17 Feb 2009 18:06:20
} -0000
} 14, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com)
} (198.176.16.26)
} 14, 34 -- by server-2.tower-96.messagelabs.com with RC4-SHA encrypted SMTP;
} 17 Feb 2009 18:06:20 -0000
} 14, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by
} naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
} 14, 34 -- Tue, 17 Feb 2009 12:06:19 -0600
} 14, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 14, 34 -- Content-class: urn:content-classes:message
} 14, 34 -- MIME-Version: 1.0
} 14, 34 -- Content-Type: text/plain;
} 14, 34 -- charset="US-ASCII"
} 14, 34 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades
} 14, 34 -- Date: Tue, 17 Feb 2009 12:03:14 -0600
} 14, 34 -- Message-ID:
} {66260BA266051B4FA0EC9EA3B33E6A9401B6CA4A-at-naoaksasebe02.nr.ad.newellco.com}
} 14, 34 -- In-Reply-To: {200902171722.n1HHME0D026703-at-ns.microscopy.com}
} 14, 34 -- X-MS-Has-Attach:
} 14, 34 -- X-MS-TNEF-Correlator:
} 14, 34 -- Thread-Topic: [Microscopy] Re: EM - Cross-sections of paper grades
} 14, 34 -- Thread-Index: AcmRJFwPAdBdcY/AR2SFHvrxwphFVQAAm65Q
} 14, 34 -- References: {200902171722.n1HHME0D026703-at-ns.microscopy.com}
} 14, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
} 14, 34 -- To: {oshel1pe-at-cmich.edu}
} 14, 34 -- Cc: {Microscopy-at-microscopy.com}
} 14, 34 -- X-OriginalArrivalTime: 17 Feb 2009 18:06:19.0704 (UTC)
} FILETIME=[6F330B80:01C9912A]
} 14, 34 -- Content-Transfer-Encoding: 8bit
} 14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n1HI6NIO009186
} ==============================End of - Headers==============================



==============================Original Headers==============================
13, 46 -- From prvs=2925deefe=Rosemary.White-at-csiro.au Tue Feb 17 15:40:05 2009
13, 46 -- Received: from vic-MTAout3.csiro.au (vic-MTAout3.csiro.au [150.229.64.39])
13, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HLe3Dx027301
13, 46 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 15:40:04 -0600
13, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
13, 46 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
13, 46 -- s=email; t=1234906805; x=1266442805;
13, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
13, 46 -- mime-version:content-transfer-encoding:content-id:
13, 46 -- content-description:resent-date:resent-from:resent-sender:
13, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
13, 46 -- references:list-id:list-help:list-unsubscribe:
13, 46 -- list-subscribe:list-post:list-owner:list-archive;
13, 46 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
13, 46 -- |Subject:=20Re:=20[Microscopy]=20=20EM=20-=20Cross-sectio
13, 46 -- ns=20of=20paper=20grades|Date:=20Wed,=2018=20Feb=202009
13, 46 -- =2008:39:59=20+1100|Message-ID:=20 {C5C179DF.37FD%rosemary
13, 46 -- .white-at-csiro.au} |To:=20"Robert.Zonis-at-Sanford.com"=20 {Robe
13, 46 -- rt.Zonis-at-Sanford.com} ,=0D=0A=09 {Microscopy-at-microscopy.com
13, 46 -- } |MIME-Version:=201.0|Content-Transfer-Encoding:=207bit
13, 46 -- |In-Reply-To:=20 {200902171813.n1HIDBXf018399-at-ns.microscop
13, 46 -- y.com} ;
13, 46 -- bh=TiR8U0W0uxFosqaW9y5Z/Jmi5O+6dNlXGAFxIW0Vw/c=;
13, 46 -- b=aHDF4vttpwkWqI9tP4ck235KrFkn4Rxv9580Sv0I1Gln+NxSUPm+U4/+
13, 46 -- IcNgqvzq+FfvueKC7OAP+0k79st3vWzZAmYbiGIb5ft+cdURWYXqpm/Nk
13, 46 -- Cf00OUYnR+d3uOa;
13, 46 -- X-IronPort-AV: E=Sophos;i="4.38,225,1233493200";
13, 46 -- d="scan'208";a="9636926"
13, 46 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
13, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 18 Feb 2009 08:40:01 +1100
13, 46 -- Received: from [152.83.167.123] (152.83.167.123) by
13, 46 -- exvic-htca01.nexus.csiro.au (138.194.81.126) with Microsoft SMTP Server id
13, 46 -- 8.1.340.0; Wed, 18 Feb 2009 08:40:00 +1100
13, 46 -- User-Agent: Microsoft-Entourage/12.10.0.080409
13, 46 -- Date: Wed, 18 Feb 2009 08:39:59 +1100
13, 46 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades
13, 46 -- From: Rosemary White {rosemary.white-at-csiro.au}
13, 46 -- To: "Robert.Zonis-at-Sanford.com" {Robert.Zonis-at-Sanford.com} ,
13, 46 -- {Microscopy-at-microscopy.com}
13, 46 -- Message-ID: {C5C179DF.37FD%rosemary.white-at-csiro.au}
13, 46 -- Thread-Topic: [Microscopy] EM - Cross-sections of paper grades
13, 46 -- Thread-Index: AcmRK2miy7CImIrFQuyLfywxlS1LwAAHN51Y
13, 46 -- In-Reply-To: {200902171813.n1HIDBXf018399-at-ns.microscopy.com}
13, 46 -- MIME-Version: 1.0
13, 46 -- Content-Type: text/plain; charset="US-ASCII"
13, 46 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jacqueline.ayotte-at-ticona.com
Date: Tue, 17 Feb 2009 15:57:28 -0600
Subject: [Microscopy] viaWWW: Microtoming glass filled polymers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jacqueline.ayotte-at-ticona.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jacqueline.ayotte-at-ticona.com
Name: Jacqueline Ayotte

Organization: Ticona Engineering Polymers

Title-Subject: [Filtered] Microtoming glass filled polymers

Question: I am interested in being able to microtome glass filled
polymers. I currently microtome using glass knives, which works well
for unfilled polymeric materials but not glass filled. My end goal is
to view crystalline morphology of glass filled polymers as I
currently do with unfilled polymers.

Does anyone currently do this and what equipment are you using?

Thank you for any information or pointers.

Jacqueline

Login Host: 63.241.226.130
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Feb 17 15:57:28 2009
9, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HLvPqE009193
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 15:57:27 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c5c0e3357f30-at-[130.194.133.34]}
9, 11 -- Date: Wed, 18 Feb 2009 08:57:21 +1100
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: jacqueline.ayotte-at-ticona.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Microtoming glass filled polymers
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DGambaro-at-memc.it
Date: Wed, 18 Feb 2009 05:08:22 -0600
Subject: [Microscopy] SEM - Need help on Topcon sm510 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

We have a SEM Topcon SM510 that needs some help: our monitor board burned.
I am wondering if anyone else suffered such a disaster and can suggest us
where we can repair the CRT or buy another equivalent monitor ?
Feel free to contact me off list.

Thanks for your help
Best Regards

Daniela Gambaro



==============================Original Headers==============================
7, 24 -- From DGambaro-at-memc.it Wed Feb 18 05:08:22 2009
7, 24 -- Received: from mail.memc.it (host18-139-static.43-88-b.business.telecomitalia.it [88.43.139.18])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IB8Lj1009599
7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 05:08:22 -0600
7, 24 -- Received: from novlnml1.eur.memc.com ([167.170.160.16])
7, 24 -- by mail.memc.it (Lotus Domino Release 6.5.6FP3)
7, 24 -- with ESMTP id 2009021812082100-11084 ;
7, 24 -- Wed, 18 Feb 2009 12:08:21 +0100
7, 24 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 24 -- MIME-Version: 1.0
7, 24 -- Subject: SEM - Need help on Topcon sm510 repair
7, 24 -- To: {Microscopy-at-microscopy.com}
7, 24 -- From: DGambaro-at-memc.it
7, 24 -- Message-ID: {OFA7E4DE46.6EBED0FE-ONC1257561.003989C6-C1257561.003D30B2-at-memc.com}
7, 24 -- Date: Wed, 18 Feb 2009 12:08:21 +0100
7, 24 -- X-MIMETrack: Serialize by Router on NOVLNML1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at
7, 24 -- 18/02/2009 12.08.20,
7, 24 -- Serialize complete at 18/02/2009 12.08.20,
7, 24 -- Itemize by SMTP Server on NOVLNHB1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at
7, 24 -- 18/02/2009 12.08.21,
7, 24 -- Serialize by Router on NOVLNHB1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at
7, 24 -- 18/02/2009 12.08.22,
7, 24 -- Serialize complete at 18/02/2009 12.08.22
7, 24 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: rbeavers-at-mail.smu.edu
Date: Wed, 18 Feb 2009 12:43:47 -0600
Subject: [Microscopy] Laser Surface Profiler

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Group,

Trying to locate a lab in the North Texas area that has Laser 3D Surface profile capabilities. Need to profile surface of a laser treated polyimide film on glass substrate to nanometer resolution. SEM images have been very difficult to achieve due to charging and small feature size. Thinking a surface profiler or AFM may give better results.

Prefer University labs but will be glad to hear from commercial labs as well.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
7, 26 -- From rbeavers-at-mail.smu.edu Wed Feb 18 12:43:46 2009
7, 26 -- Received: from sxet1p1.systems.smu.edu (sxet1p1.systems.smu.edu [129.119.65.146])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IIhhhn011183
7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:43:46 -0600
7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by
7, 26 -- sxet1p1.systems.smu.edu (129.119.65.146) with Microsoft SMTP Server (TLS) id
7, 26 -- 8.1.340.0; Wed, 18 Feb 2009 12:43:50 -0600
7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Wed, 18 Feb 2009
7, 26 -- 12:43:40 -0600
7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 26 -- Date: Wed, 18 Feb 2009 12:43:39 -0600
7, 26 -- Subject: Laser Surface Profiler
7, 26 -- Thread-Topic: Laser Surface Profiler
7, 26 -- Thread-Index: AcmR+NCtXIRxFtvWRPKhtObGHr6YEg==
7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB053347F142-at-SXMBXC.systems.smu.edu}
7, 26 -- Accept-Language: en-US
7, 26 -- Content-Language: en-US
7, 26 -- X-MS-Has-Attach:
7, 26 -- X-MS-TNEF-Correlator:
7, 26 -- acceptlanguage: en-US
7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
7, 26 -- MIME-Version: 1.0
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1IIhhhn011183
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Wed, 18 Feb 2009 13:22:08 -0600
Subject: [Microscopy] Laser Surface Profiler

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Couple of year ago I used services from Optipro www.optipro.com, they sell
laser profiling equipment and did provide lab services for a reasonable fee;
not in TX though, NY.

I worked with Lynda Bechtold lynda-at-optipro.com

No commercial interest, just a satisfied customer :)

Valery Ray

============================
www.partbeamsystech.com
PBS&T, MEO Engineering Co, Inc.
290 Broadway St., Suite 298
Methuen, MA 01844
Phone: (978) 296-5063


-----Original Message-----
X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
Sent: Wednesday, February 18, 2009 1:48 PM
To: vray-at-partbeamsystech.com

Group,

Trying to locate a lab in the North Texas area that has Laser 3D Surface
profile capabilities. Need to profile surface of a laser treated polyimide
film on glass substrate to nanometer resolution. SEM images have been very
difficult to achieve due to charging and small feature size. Thinking a
surface profiler or AFM may give better results.

Prefer University labs but will be glad to hear from commercial labs as
well.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
7, 26 -- From rbeavers-at-mail.smu.edu Wed Feb 18 12:43:46 2009
7, 26 -- Received: from sxet1p1.systems.smu.edu (sxet1p1.systems.smu.edu
[129.119.65.146])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1IIhhhn011183
7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:43:46
-0600
7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by
7, 26 -- sxet1p1.systems.smu.edu (129.119.65.146) with Microsoft SMTP
Server (TLS) id
7, 26 -- 8.1.340.0; Wed, 18 Feb 2009 12:43:50 -0600
7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Wed, 18 Feb
2009
7, 26 -- 12:43:40 -0600
7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 26 -- Date: Wed, 18 Feb 2009 12:43:39 -0600
7, 26 -- Subject: Laser Surface Profiler
7, 26 -- Thread-Topic: Laser Surface Profiler
7, 26 -- Thread-Index: AcmR+NCtXIRxFtvWRPKhtObGHr6YEg==
7, 26 -- Message-ID:
{7A6FE75608A3624E872147993C8B36BB053347F142-at-SXMBXC.systems.smu.edu}
7, 26 -- Accept-Language: en-US
7, 26 -- Content-Language: en-US
7, 26 -- X-MS-Has-Attach:
7, 26 -- X-MS-TNEF-Correlator:
7, 26 -- acceptlanguage: en-US
7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
7, 26 -- MIME-Version: 1.0
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1IIhhhn011183
==============================End of - Headers==============================



==============================Original Headers==============================
20, 26 -- From vray-at-partbeamsystech.com Wed Feb 18 13:22:08 2009
20, 26 -- Received: from smtp108.biz.mail.re2.yahoo.com (smtp108.biz.mail.re2.yahoo.com [206.190.52.47])
20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1IJM63B026685
20, 26 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 13:22:07 -0600
20, 26 -- Message-Id: {200902181922.n1IJM63B026685-at-ns.microscopy.com}
20, 26 -- Received: (qmail 3741 invoked from network); 18 Feb 2009 19:22:04 -0000
20, 26 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.106.16 with login)
20, 26 -- by smtp108.biz.mail.re2.yahoo.com with SMTP; 18 Feb 2009 19:22:04 -0000
20, 26 -- X-YMail-OSG: LFPYeiEVM1kPdrQGo7aG.yFJOOwBa_IXD7U8E5vVfihPd8u3yCSQy0KM_2b8MN0Q8vjRGBrDQ_MD9Ic71UjiEYoF4v0SufDHBXYRpRexrEXpBV3iQ5l2vgk0R5oWFmpybVf18O2mdZ62R26eBQvpa5xBiO7._tvuxFoXO03sCEg4YPyrI1nkr24Yu5T9VXCwu2isao2fLJw7FK95uPG7TZf4
20, 26 -- X-Yahoo-Newman-Property: ymail-3
20, 26 -- Reply-To: {vray-at-partbeamsystech.com}
20, 26 -- From: "Valery Ray" {vray-at-partbeamsystech.com}
20, 26 -- To: {rbeavers-at-mail.smu.edu}
20, 26 -- Cc: {microscopy-at-microscopy.com}
20, 26 -- Subject: RE: [Microscopy] Laser Surface Profiler
20, 26 -- Date: Wed, 18 Feb 2009 14:24:57 -0500
20, 26 -- Organization: PBST / MEO Engineering
20, 26 -- MIME-Version: 1.0
20, 26 -- Content-Type: text/plain;
20, 26 -- charset="iso-8859-1"
20, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
20, 26 -- Thread-Index: AcmR+V0Ama8AvtQtTeCfdtTcSAmpkgABCnTg
20, 26 -- In-Reply-To: {200902181847.n1IIlYnL012819-at-ns.microscopy.com}
20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 26 -- Content-Transfer-Encoding: 8bit
20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1IJM63B026685
==============================End of - Headers==============================




From: cell.toli-at-gmail.com
Date: Wed, 18 Feb 2009 14:01:06 -0600
Subject: [Microscopy] Rare Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello I am in search for 4 inch wafers for a e-beam lithography research
project, I have been searching for the past six months with no luck.

Dopant Orient. Resistance (ohm-cm)
Phosphorus 1-0-0 0.1 Boron 1-0-0 0.1 Phosphorus 1-1-1 0.1 Boron 1-1-1
0.1 Side -Silicon w/ no film/coating (natural oxide up to 50nm ok) Other
side - galvanic contact pad to base w/ AL coating

Wafer thickness cannot exceed 1mm Wafer thickness cannot be less than 0.3mm

Also am seeking for micro silica of the above wafers, if available. This
is an ASAP matter, please contact me soon.

Could anyone provide possible sources to locate odd and specialty wafers?

Best Regards,

Anatoli Oleynik
Specialty Consultant
ProBiz Consulting

==============================Original Headers==============================
7, 37 -- From cell.toli-at-gmail.com Wed Feb 18 14:01:05 2009
7, 37 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.150])
7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IK14s0009391
7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 14:01:04 -0600
7, 37 -- Received: by qw-out-1920.google.com with SMTP id 14so20699qwa.54
7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:01:03 -0800 (PST)
7, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 37 -- d=gmail.com; s=gamma;
7, 37 -- h=domainkey-signature:received:received:message-id:date:from
7, 37 -- :user-agent:mime-version:to:subject:content-type
7, 37 -- :content-transfer-encoding;
7, 37 -- bh=d4aFgc5r/lyem2WGLj/OerFnq7wDSc9PYNA2RgU5+QI=;
7, 37 -- b=jBRIpDZLX70J5HI5A94DutjpwyO9sgTv+agfgj/vP5C+KjDfo44g7WrnXu927m9JBD
7, 37 -- rv3qiX5d5bTO+FkSvYS0Ezj10xAyYDxuOZdmT0FbsURBasLe0hgGVlFJqoPBQ9J+mk3p
7, 37 -- 5BNU5DEukMBK5bAHyJWV4nKzBz50K8dAtdhFI=
7, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 37 -- d=gmail.com; s=gamma;
7, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject
7, 37 -- :content-type:content-transfer-encoding;
7, 37 -- b=TIoNrfjN/UVBg/eeDcyZ/zMRUg9eUY+pn5wRqGtHlMgD9xsqZ1nZ5CcUDLqTJ2dAvr
7, 37 -- ZXm0LBtGr7oZqX/0LolkgYtG0Asq9zEWlcxVhr0suNvjLYEGqPX2Tysed3iUM5A7TtwJ
7, 37 -- WCxfGvFYKDfjPFNemWI+E+F6pqRWUhwyLOUJQ=
7, 37 -- Received: by 10.229.85.17 with SMTP id m17mr3260973qcl.43.1234987259004;
7, 37 -- Wed, 18 Feb 2009 12:00:59 -0800 (PST)
7, 37 -- Received: from ?127.0.0.1? (cpe-065-190-187-114.nc.res.rr.com [65.190.187.114])
7, 37 -- by mx.google.com with ESMTPS id 8sm417133ywg.54.2009.02.18.12.00.58
7, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
7, 37 -- Wed, 18 Feb 2009 12:00:58 -0800 (PST)
7, 37 -- Message-ID: {499C68FC.9020500-at-gmail.com}
7, 37 -- Date: Wed, 18 Feb 2009 15:01:00 -0500
7, 37 -- From: Anatoli Oleynik {cell.toli-at-gmail.com}
7, 37 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105)
7, 37 -- MIME-Version: 1.0
7, 37 -- To: Microscopy-at-microscopy.com
7, 37 -- Subject: Rare Wafers
7, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 37 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: susan.trant-at-viha.ca
Date: Wed, 18 Feb 2009 15:55:15 -0600
Subject: [Microscopy] viaWWW: Knife Makers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both susan.trant-at-viha.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: susan.trant-at-viha.ca
Name: Susan Trant

Organization: Vancouver Island Health Authority

Title-Subject: [Filtered] Knife Makers

Question: I have an older LKB Bromma 7800 knife maker. I am looking
for a vendor for the scoring blades. Does anyone know where I can
purchase some more?

Sue Trant
EM Technologist
Vancouver Island Health Authority


Login Host: 207.194.133.9
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Feb 18 15:55:14 2009
8, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ILt9hg027769
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 15:55:13 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p0624080ec5c234255aa5-at-[130.194.133.34]}
8, 11 -- Date: Thu, 19 Feb 2009 08:55:04 +1100
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: susan.trant-at-viha.ca (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Knife Makers
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Wed, 18 Feb 2009 18:47:55 -0600
Subject: [Microscopy] viaWWW: TEM Philips CM120 Holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: David Mitchell

Organization: EM Unit, University of Sydney

Title-Subject: [Filtered] TEM Philips CM120 Holders

Question: We have a venerable Philips TEM: CM120 Biofilter. This has
been used mainly for biological work, but we have some materials folk
wanting to look at ferromagnetic materials. For this we need holders
with screw inserts, to ensure the foils don't get dislodged by the
field (we've already had one disappear in the microscope, from the
standard spring loaded holder). Being such an old model, I was
wondering if any users had compatible holders from decommissioned
machines they might be interested in passing on? We also have an even
older CM12, and similar holders for this might also help us out. I am
happy to discuss shipping charges - please email me directly. Thanks
in advance and regards.

Dave Mitchell
TEM Manager, EMU, University of Sydney

Login Host: 129.78.220.7
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Feb 18 18:47:54 2009
7, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1J0lqpu015370
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 18:47:54 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p0624080fc5c25c90d3a7-at-[130.194.133.34]}
7, 11 -- Date: Thu, 19 Feb 2009 11:47:43 +1100
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: TEM Philips CM120 Holders
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Thu, 19 Feb 2009 11:00:58 -0600
Subject: [Microscopy] Re: [a] Rare Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anatoli Oleynik asked about a source for unusual Silicon and silica wafers.
Try contacting Chris Baker of University Wafers at:
Visit http://www.wafersale.com or email chris-at-wafersale.com
or call Call 1-800-216-8349 / Fax 888-832-0340



disclaimer: I have no relationship with this company, even as a customer.
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================
----- Original Message -----
From: cell.toli-at-gmail.com
To: donc-at-asmicro.com
Sent: Wednesday, February 18, 2009 3:06 PM
Subject: [a] [Microscopy] Rare Wafers





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Hello I am in search for 4 inch wafers for a e-beam lithography research
project, I have been searching for the past six months with no luck.

Dopant Orient. Resistance (ohm-cm)
Phosphorus 1-0-0 0.1 Boron 1-0-0 0.1 Phosphorus 1-1-1 0.1 Boron 1-1-1
0.1 Side -Silicon w/ no film/coating (natural oxide up to 50nm ok) Other
side - galvanic contact pad to base w/ AL coating

Wafer thickness cannot exceed 1mm Wafer thickness cannot be less than
0.3mm

Also am seeking for micro silica of the above wafers, if available. This
is an ASAP matter, please contact me soon.

Could anyone provide possible sources to locate odd and specialty wafers?

Best Regards,

Anatoli Oleynik
Specialty Consultant
ProBiz Consulting

==============================Original
Headers==============================
7, 37 -- From cell.toli-at-gmail.com Wed Feb 18 14:01:05 2009
7, 37 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com
[74.125.92.150])
7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1IK14s0009391
7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 14:01:04 -0600
7, 37 -- Received: by qw-out-1920.google.com with SMTP id 14so20699qwa.54
7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009
12:01:03 -0800 (PST)
7, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 37 -- d=gmail.com; s=gamma;
7, 37 --
h=domainkey-signature:received:received:message-id:date:from
7, 37 -- :user-agent:mime-version:to:subject:content-type
7, 37 -- :content-transfer-encoding;
7, 37 -- bh=d4aFgc5r/lyem2WGLj/OerFnq7wDSc9PYNA2RgU5+QI=;
7, 37 --
b=jBRIpDZLX70J5HI5A94DutjpwyO9sgTv+agfgj/vP5C+KjDfo44g7WrnXu927m9JBD
7, 37 --
rv3qiX5d5bTO+FkSvYS0Ezj10xAyYDxuOZdmT0FbsURBasLe0hgGVlFJqoPBQ9J+mk3p
7, 37 -- 5BNU5DEukMBK5bAHyJWV4nKzBz50K8dAtdhFI=
7, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 37 -- d=gmail.com; s=gamma;
7, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject
7, 37 -- :content-type:content-transfer-encoding;
7, 37 --
b=TIoNrfjN/UVBg/eeDcyZ/zMRUg9eUY+pn5wRqGtHlMgD9xsqZ1nZ5CcUDLqTJ2dAvr
7, 37 --
ZXm0LBtGr7oZqX/0LolkgYtG0Asq9zEWlcxVhr0suNvjLYEGqPX2Tysed3iUM5A7TtwJ
7, 37 -- WCxfGvFYKDfjPFNemWI+E+F6pqRWUhwyLOUJQ=
7, 37 -- Received: by 10.229.85.17 with SMTP id
m17mr3260973qcl.43.1234987259004;
7, 37 -- Wed, 18 Feb 2009 12:00:59 -0800 (PST)
7, 37 -- Received: from ?127.0.0.1? (cpe-065-190-187-114.nc.res.rr.com
[65.190.187.114])
7, 37 -- by mx.google.com with ESMTPS id
8sm417133ywg.54.2009.02.18.12.00.58
7, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
7, 37 -- Wed, 18 Feb 2009 12:00:58 -0800 (PST)
7, 37 -- Message-ID: {499C68FC.9020500-at-gmail.com}
7, 37 -- Date: Wed, 18 Feb 2009 15:01:00 -0500
7, 37 -- From: Anatoli Oleynik {cell.toli-at-gmail.com}
7, 37 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105)
7, 37 -- MIME-Version: 1.0
7, 37 -- To: Microscopy-at-microscopy.com
7, 37 -- Subject: Rare Wafers
7, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 37 -- Content-Transfer-Encoding: 7bit
==============================End of -
Headers==============================


==============================Original Headers==============================
18, 25 -- From donc-at-asmicro.com Thu Feb 19 11:00:58 2009
18, 25 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1JH0vrf029985
18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 11:00:57 -0600
18, 25 -- Received: (qmail 59861 invoked from network); 19 Feb 2009 17:00:57 -0000
18, 25 -- Received: from unknown (HELO asm15) (donc-at-76.240.194.228 with login)
18, 25 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 19 Feb 2009 17:00:56 -0000
18, 25 -- X-YMail-OSG: usQbrN0VM1mjAkpGFX8JohEOdoXLuo6e2QrFqK4U4kJp3.ITYR1HcR8.CYOd20mGiSl3ZITmNKU2CHxK4CrkRdzqBz_.1p.Wx9r1aCQ47eKE_hvJcnQsMA5Os7dZbkxQDV_YDeij7m9_bGbiyZGxlcZGARldlVYoyhgea2GHcApRakdduRtZapmcXMjJRvWrLGNhtt_O3ak5RAgJqKMdyHwJcQzz
18, 25 -- X-Yahoo-Newman-Property: ymail-3
18, 25 -- Message-ID: {E12AC932284949C79C69D20CE5B324CD-at-asm15}
18, 25 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
18, 25 -- To: {cell.toli-at-gmail.com} , "Microscopy List" {microscopy-at-microscopy.com}
18, 25 -- References: {200902182006.n1IK6352014039-at-ns.microscopy.com}
18, 25 -- Subject: Re: [a] [Microscopy] Rare Wafers
18, 25 -- Date: Thu, 19 Feb 2009 12:00:40 -0500
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain;
18, 25 -- format=flowed;
18, 25 -- charset="iso-8859-1";
18, 25 -- reply-type=original
18, 25 -- Content-Transfer-Encoding: 7bit
18, 25 -- X-Priority: 3
18, 25 -- X-MSMail-Priority: Normal
18, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Thu, 19 Feb 2009 18:12:11 -0600
Subject: [Microscopy] Position Open: Director, CAMCOR Transmission Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Director, CAMCOR Transmission Electron Microscopy Facility
The Center for Advanced Materials Characterization in Oregon, the
Materials Science Institute (MSI) at the University of Oregon (UO) in
Eugene in partnership with the Oregon Nanoscience and
Microtechnologies Institute (ONAMI) is currently seeking applications
for a Director for CAMCOR's Transmission Electron Microscopy (TEM)
Facility. The position of Director for the CAMCOR TEM Facility
requires a high energy, responsible, efficient and meticulous
manager/analyst that is focused on ensuring that the facility is
reliably operational and available to all faculty, students and
commercial users on a daily basis. The Director is required to manage
the facility budget and ensure that a sound business model is
implemented. A vision for making the facility a leader in the
northwest and passion for expanding the impact of the facility among
ONAMI researchers is desired. A PhD in chemistry, physics, materials
science, or related field is required or five years experience as
facilities director of a TEM facility. Expertise in electron
diffraction, imaging techniques, analytical techniques, and
high-resolution electron microscopy (HREM) is required. Experience
working with aberration corrected TEM is preferred. The successful
candidate will have the ability to work effectively with faculty,
staff and students from a variety of diverse backgrounds. Applicants
should submit a cover letter, curriculum vitae and arrange for three
reference letters to be sent electronically to Carol Hanson at
carolhan-at-uoregon.edu. Review will begin 3/20/09 and position will
remain open until filled. For full position announcement, see

http://hr.uoregon.edu/jobs/unclassified.php?subtype=academic

An equal-opportunity, affirmative-action institution committed to
cultural diversity and compliance with the Americans with Disabilities Act.

POSITION DESCRIPTION
Terms of Appointment
Position title: Director, CAMCOR Transmission Electron Microscopy Facility
Appointment percent: 0.8 fte
Type of appointment: Fixed-term
Annual base rate/range: $70,000-100,000
Annual Basis: 12
Starting date: April 2009

Essential Functions
The Center for Advanced Materials Characterization in Oregon, the
Materials Science Institute (MSI) at the University of Oregon (UO) in
Eugene in partnership with the Oregon Nanoscience and
Microtechnologies Institute (ONAMI) is currently seeking applications
for a Director for CAMCOR's Transmission Electron Microscopy (TEM) Facility.

The position of Director for the CAMCOR TEM Facility requires a high
energy, responsible, efficient and meticulous manager/analyst that is
focused on ensuring that the facility is reliably operational and
available to all faculty, students and commercial users on a daily
basis. The Director is required to manage the facility budget and
ensure that a sound business model is implemented. A vision for
making the facility a leader in the northwest and passion for
expanding the impact of the facility among ONAMI researchers is desired.

Minimum and Preferred Qualifications
A PhD in chemistry, physics, materials science, or related field is
required or five years experience as facilities director of a TEM
facility. Expertise in electron diffraction, imaging techniques,
analytical techniques, and high-resolution electron microscopy (HREM)
is required. Experience working with aberration corrected TEM is preferred.

Description of the University and the Community
Located 110 miles south of Portland, the University of Oregon has an
enrollment of ~20,000 students. The Eugene metro area (pop. 215,000)
is in a region noted for its dynamic quality of life and progressive
cultural environment. We are about an hour's drive from the Pacific
coast and the crest of the Cascade Mountains. The University is an
AAU research institution and a member of the Pac-10 conference.

Commitment to Affirmative Action and Equal Opportunity
The successful candidate will have the ability to work effectively
with faculty, staff and students from a variety of diverse backgrounds.

Application Procedure and Closing Dates
Applicants should submit a cover letter, curriculum vitae and arrange
for three reference letters to be sent electronically to Carol Hanson
at carolhan-at-uoregon.edu. Review will begin 3/20/09 and position will
remain open until filled.

JOB DESCRIPTION

The position of Director for the CAMCOR TEM Facility requires a high
energy, responsible, efficient and meticulous manager/analyst that is
focused on ensuring that the facility is reliably operational and
available to all faculty, students and commercial users on a daily
basis. The Director is required to manage the facility budget, ensure
that a sound business model is implemented that provides the
necessary income level sufficient to cover operating costs and
salaries and provide vision for the facility. The director will need
to know how to get many types ofTEM data, understand what types of
TEM data are most useful for specific problems, and work with
professors and industries from different disciplines and sell the use
of TEM as important to their work.

The main areas of responsibility are: Technical, Management,
Training, Budgetary, Promotion, Scientific, Education, Operational,
Documentation, Service and Cooperation. The following are some
specific examples of these:
1. Technical
- Familiar with all procedures and techniques
- Operate all instruments and equipment professionally

2. Management
- Oversee budget and purchases
- Student and staff employees

3. Training
- Train student, staff and instrument and laboratory users
- Assist faculty and other users

4. Promotion
- Improve lab visibility though various mechanisms
- Workshops
- Web pages
- Commercial visits and lectures

5. Scientific
- Attend scientific conferences
- Publish peer reviewed papers

6. Education
- Teach UofO courses

7. Operational
- Ensure day to day operational reliability and staffing

8. Documentation
- Keep log and sample notebooks
- Write lecture notes, training and reference manuals
- Technical notes

9. Service
- Provide "fee for service" for commercial work
- Provide "public" access
- Provide tours and guided explanations to misc visitors other UofO classes

10. Cooperation
- Work with faculty to determine technical goals, write new
instrumentation proposals, recruit new faculty and graduate students

Specific examples:
1. Ensure that state-of-the-art instrumentation is available and
replaced on a cyclical basis by organizing the design and writing of
government and private grant proposals. This requires working with
faculty to identify analytical needs of the UofO faculty research
programs that are suitable for a multi-user facility and ideally
offer a capability that other four year colleges and commercial
interests can also utilize, to provide additional outside income.
2. Design instrumentation specification and performance guidelines
for instrument purchases. Work with faculty and the UofO business
office and legal office to obtain scientific instrumentation that is
optimized for UofO research needs and pushes the vendor engineering
to the current limit of ultimate performance. At the same time, work
with multiple vendors to obtain the lowest possible cost to UofO.
3. Manage and train both part-time students and full-time staff to
ensure that all analytical practices are adhered to in a rigorous and
quantitative manner. Ensure that all procedures are properly
documented and available.
4. Manage the daily operation of the facility by ensuring that all
consumables and supplies are freely available and ready for immediate
usage. See that all computers, scanners, microscopes and tools are
supplied and updated as necessary.
5. Ensure that all software applications for both data and image
acquisition and also off-line reprocessing are specified and
configured to provide the maximum efficiency and ease of use for the
best possible utility. Work with all software vendors to implement
improvements and suggestions in the accuracy, usability and technical
aspects of the software.
6. Design and teach courses in the relevant technical areas of
facility to promote students' theoretical and practical understanding
of transmission electron microscopy (TEM), electron diffraction,
imaging techniques, analytical techniques, and high-resolution
electron microscopy (HREM). Update and publish lecture notes,
research papers and technical "whitepapers" to improve and advance
knowledge in the field.
7. Attend conferences and give presentations and posters to promote
the facility and also to learn new advances in the field and new
ideas that might prove useful to the facility. Maintain professional
contacts with other laboratory managers and exchange ideas and
technical know-how.
8. Ensure that all instruments are properly serviced, maintained and
upgraded or improved if possible. Work with UofO CAMCOR and TSA
service technicians to ensure that the instruments are given priority
repairs and regular maintenance to provide maximum reliability.
9. Implement automation procedures for all data acquisition to the
maximum extent possible to ensure round the clock instrument usage.
This may require working with OEM and third party software vendors to
negotiate new capabilities in the application software to achieve this goal.
10. Promote, advertise and circulate literature and "glossy"
brochures to all interested UofO and OUS faculty in general to
maintain maximum utilization of the instrument schedule. Maintain a
web-based user schedule for users to improve scheduling efficiency.
11. Maintain supplies and equipment for efficient sample.
12. Operate all instruments for commercial fee for service usage as
scheduled or on an emergency basis for time critical work. Process
all data to highest levels of professional skill and accuracy.
Prepare documentation of all procedures, work, analysis configuration
and acquisition and data reprocessing providing precision estimates
and accuracy to the best of ability. Ensure that all commercial
samples and data are protected and secured.


==============================Original Headers==============================
25, 20 -- From donovan-at-uoregon.edu Thu Feb 19 18:12:11 2009
25, 20 -- Received: from smtp.uoregon.edu (mserv3.uoregon.edu [128.223.142.101])
25, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K0CArZ030200
25, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 18:12:10 -0600
25, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
25, 20 -- (authenticated bits=0)
25, 20 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n1K0C9h5018158
25, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
25, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 16:12:10 -0800
25, 20 -- Message-Id: {200902200012.n1K0C9h5018158-at-smtp.uoregon.edu}
25, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
25, 20 -- Date: Thu, 19 Feb 2009 16:11:54 -0800
25, 20 -- To: microscopy-at-microscopy.com
25, 20 -- From: John Donovan {donovan-at-uoregon.edu}
25, 20 -- Subject: Position Open: Director, CAMCOR Transmission Electron
25, 20 -- Microscopy Facility, University of Oregon, Eugene, Oregon
25, 20 -- Mime-Version: 1.0
25, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
25, 20 -- X-Virus-Scanned: ClamAV 0.94.2/9015/Thu Feb 19 10:30:06 2009 on mserv3
25, 20 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Thu, 19 Feb 2009 19:23:45 -0600
Subject: [Microscopy] SEM of Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Naomi,

X-from your question it appears you are more advanced than us when it comes to
studying hair specimens. We receive one or two hair specimens per year so
our experience is quite limited.
We don't follow any diagnostic algorithm and I can't comment on your
question about telogen effluvium. Maybe someone else here on the forum can
help.
We examine the hair shaft and cuticle for structural defects by SEM and
examine the hair shaft through polarised light by LM. Other than that we
don't have any advanced methodology.

Regards,

John Brealey
Supervising Scientist
EM Unit
Queen Elizabeth Hospital
SA Pathology
South Australia


Hi John

It was interesting to follow your comments on the Microscopy Listserver as
we are in a similar position here at Pathology Queensland. At present out
pathologist is requesting Whole Mount for polarised LM and then SEM on every
specimen that walks through the door.

I was curious to know if you follow a diagnostic algorithm for Hair
specimens. Eg for Telogen Effluvium is it considered necessary to go to SEM
or just tally ratio of root bulbs at LM?

Thank you for your contributions
Naomi

Naomi McCallum
Supervising Scientist
Electron Microscope Unit
Anatomical Pathology & Cytopathology
PATHOLOGY QUEENSLAND
Central Laboratory
RBWH



==============================Original Headers==============================
8, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 19:23:44 2009
8, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13])
8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K1Nhk2013286
8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 19:23:44 -0600
8, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000";
8, 27 -- d="scan'208";a="11377363"
8, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88])
8, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 11:53:42 +1030
8, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
8, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id
8, 27 -- 8.1.263.0; Fri, 20 Feb 2009 11:54:32 +1030
8, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
8, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 87A8F34C34 for
8, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 11:53:42 +1030 (CST)
8, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
8, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
8, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 27 -- Subject: SEM of Hair
8, 27 -- Date: Fri, 20 Feb 2009 11:53:43 +1030
8, 27 -- Organization: IMVS
8, 27 -- Message-ID: {000001c992f9$de8af350$7d8a140a-at-41347i}
8, 27 -- MIME-Version: 1.0
8, 27 -- Content-Type: text/plain; charset="us-ascii"
8, 27 -- Content-Transfer-Encoding: 7bit
8, 27 -- X-Mailer: Microsoft Office Outlook 11
8, 27 -- Thread-Index: AcmS+d5kqvxuZq0JRruEkNNJHrRNMA==
8, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Thu, 19 Feb 2009 20:00:16 -0600
Subject: [Microscopy] SEM of Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kristen,
That's a good exercise to do.
It's more interesting when you can be the guinea pig, ie, when you examine
your own hair.
Our method is really basic - we cut the hair with a sharp blade and
carefully place the pieces on a double-sided sticky tab that is stuck to an
SEM stub. Then we coat with gold in a sputter coater.

Regards,

John

Hi John,

I've been following this a bit and am a total novice in examining hair
samples with SEM. I'm always looking for good exercise for my undergrad
students to do with SEM. Would you mind sharing how you prep the samples for
SEM?

Thanks,
Kristen


--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au
{john.brealey-at-imvs.sa.gov.au} wrote:

} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au}
} Subject: [Microscopy] SEM of Hair
} To: kamlennon-at-yahoo.com
} Date: Thursday, February 19, 2009, 7:30 PM
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Hi Naomi,
}
} X-from your question it appears you are more advanced than us when it
} comes to studying hair specimens. We receive one or two hair
} specimens per year so our experience is quite limited.
} We don't follow any diagnostic algorithm and I can't comment on your
} question about telogen effluvium. Maybe someone else here on the
} forum can help.
} We examine the hair shaft and cuticle for structural defects by SEM
} and examine the hair shaft through polarised light by LM.
} Other than that we
} don't have any advanced methodology.
}
} Regards,
}
} John Brealey
} Supervising Scientist
} EM Unit
} Queen Elizabeth Hospital
} SA Pathology
} South Australia
}
}
} Hi John
}
} It was interesting to follow your comments on the Microscopy
} Listserver as we are in a similar position here at Pathology
} Queensland.
} At present out
} pathologist is requesting Whole Mount for polarised LM and then SEM on
} every specimen that walks through the door.
}
} I was curious to know if you follow a diagnostic algorithm for Hair
} specimens. Eg for Telogen Effluvium is it considered necessary to go
} to SEM or just tally ratio of root bulbs at LM?
}
} Thank you for your contributions
} Naomi
}
} Naomi McCallum
} Supervising Scientist
} Electron Microscope Unit
} Anatomical Pathology & Cytopathology
} PATHOLOGY QUEENSLAND
} Central Laboratory
} RBWH
}
}


==============================Original Headers==============================
10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009
10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K20Ep0027815
10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15 -0600
10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000";
10, 27 -- d="scan'208";a="11464458"
10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 12:30:14 +1030
10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030
10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for
10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030 (CST)
10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
10, 27 -- Subject: SEM of Hair
10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030
10, 27 -- Organization: IMVS
10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i}
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain; charset="us-ascii"
10, 27 -- Content-Transfer-Encoding: 7bit
10, 27 -- X-Mailer: Microsoft Office Outlook 11
10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g==
==============================End of - Headers==============================




From: naomi_mccallum-at-health.qld.gov.au
Date: Thu, 19 Feb 2009 22:29:35 -0600
Subject: [Microscopy] Fwd: SEM of Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

A tip that I learned from a certain SEM guru was that you get a better cross-section of the hair shaft if you fracture in liquid nitrogen. He described this technique of putting the hairs through the centre of a straw and filling the straw with liquid carbon. After freezing in Liq N2 you hit the straw with a sharp blade to fracture. There was some discussion about getting the right type of straw to make this work.

I tried this but didn't persist long enough to master it without bubbles in the straw, so I modified the technique a little. We use jewellers wire and crimps to make a 'brush' from multiple hairs (another local technique). Then we coat the hair in a sucrose blocking media used for histologic frozen sections; freeze in liq N2; fracture and then wash off the media. It works well for TS of hair shaft, you get a true fracture and not "a study in cutting techniques" (I believe that was the description).

We still use the method described below as well, mounting the LS and TS brush on the same stub.

Regards
Naomi

} } } {john.brealey-at-imvs.sa.gov.au} 20/02/2009 12:06 pm } } }



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi Kristen,
That's a good exercise to do.
It's more interesting when you can be the guinea pig, ie, when you examine
your own hair.
Our method is really basic - we cut the hair with a sharp blade and
carefully place the pieces on a double-sided sticky tab that is stuck to an
SEM stub. Then we coat with gold in a sputter coater.

Regards,

John

Hi John,

I've been following this a bit and am a total novice in examining hair
samples with SEM. I'm always looking for good exercise for my undergrad
students to do with SEM. Would you mind sharing how you prep the samples for
SEM?

Thanks,
Kristen


--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au
{john.brealey-at-imvs.sa.gov.au} wrote:

} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au}
} Subject: [Microscopy] SEM of Hair
} To: kamlennon-at-yahoo.com
} Date: Thursday, February 19, 2009, 7:30 PM
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Hi Naomi,
}
} X-from your question it appears you are more advanced than us when it
} comes to studying hair specimens. We receive one or two hair
} specimens per year so our experience is quite limited.
} We don't follow any diagnostic algorithm and I can't comment on your
} question about telogen effluvium. Maybe someone else here on the
} forum can help.
} We examine the hair shaft and cuticle for structural defects by SEM
} and examine the hair shaft through polarised light by LM.
} Other than that we
} don't have any advanced methodology.
}
} Regards,
}
} John Brealey
} Supervising Scientist
} EM Unit
} Queen Elizabeth Hospital
} SA Pathology
} South Australia
}
}
} Hi John
}
} It was interesting to follow your comments on the Microscopy
} Listserver as we are in a similar position here at Pathology
} Queensland.
} At present out
} pathologist is requesting Whole Mount for polarised LM and then SEM on
} every specimen that walks through the door.
}
} I was curious to know if you follow a diagnostic algorithm for Hair
} specimens. Eg for Telogen Effluvium is it considered necessary to go
} to SEM or just tally ratio of root bulbs at LM?
}
} Thank you for your contributions
} Naomi
}
} Naomi McCallum
} Supervising Scientist
} Electron Microscope Unit
} Anatomical Pathology & Cytopathology
} PATHOLOGY QUEENSLAND
} Central Laboratory
} RBWH
}
}


==============================Original Headers==============================
10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009
10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K20Ep0027815
10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15 -0600
10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000";
10, 27 -- d="scan'208";a="11464458"
10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 12:30:14 +1030
10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030
10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for
10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030 (CST)
10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
10, 27 -- Subject: SEM of Hair
10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030
10, 27 -- Organization: IMVS
10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i}
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain; charset="us-ascii"
10, 27 -- Content-Transfer-Encoding: 7bit
10, 27 -- X-Mailer: Microsoft Office Outlook 11
10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g==
==============================End of - Headers==============================

********************************************************************************
This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.
**********************************************************************************



==============================Original Headers==============================
22, 26 -- From prvs=13026700a6=naomi_mccallum-at-health.qld.gov.au Thu Feb 19 22:29:34 2009
22, 26 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114])
22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K4TWut012703
22, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 22:29:34 -0600
22, 26 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53])
22, 26 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id n1K4Sfqo004341
22, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:42 +1000
22, 26 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by
22, 26 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server)
22, 26 -- with ESMTP id
22, 26 -- {T8ca07f5847c0a80335e00-at-gwd-mail06.remote.health.qld.gov.au} for
22, 26 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:41 +1000
22, 26 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with
22, 26 -- Novell_GroupWise; Fri, 20 Feb 2009 14:28:41 +1000
22, 26 -- Message-Id: {499EBD0F.88BE.00AA.0-at-health.qld.gov.au}
22, 26 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
22, 26 -- Date: Fri, 20 Feb 2009 14:28:36 +1000
22, 26 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au}
22, 26 -- To: {Microscopy-at-microscopy.com}
22, 26 -- Subject: Fwd: [Microscopy] SEM of Hair
22, 26 -- References: {200902200206.n1K26CgH008879-at-ns.microscopy.com}
22, 26 -- Mime-Version: 1.0
22, 26 -- Content-Type: text/plain; charset="us-ascii"
22, 26 -- Content-Disposition: inline
22, 26 -- Content-Transfer-Encoding: 8bit
22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1K4TWut012703
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Fri, 20 Feb 2009 03:29:09 -0600
Subject: [Microscopy] Fwd: SEM of Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All

As the SEM Guru mentioned by Naomi, I thought I could add a little to the
proceedings on hair/fibres and on the earlier paper question.

Naomi has hinted at the criteria for cross sections by fracture but I will
expand. The SEM is very clever; it recognises that a material was cut with
a blunt scalpel blade and the difference between the cut from a blunt blade
and from a sharp blade. With that in mind we know that scientists often
need to quickly cross section a material with a result that is a true
representation of the material how, well crack it! Some materials will
crack naturally having been cooled in liquid nitrogen, but others will not;
hair is a good example of a difficult material to truly fracture.

When a material will not fracture naturally you need to stiffen the
material. If the material is not soluble in water we use a water soluble
carbon solution as the stiffener. Drill a fine (1/8th inch) hole though two
stubs placed face to face. Place your fibres/material through the hole and
fill the additional space with the carbon solution. When dry plunge the
unit into liquid nitrogen and when the liquid stops boiling take the unit
out and crack it by striking the interface with a single edged blade. Take
great care not to cut through the unit, simple crack it open.

Once the two units are back at room temperature and the condensation has
dispersed they are usable in the light microscope and the SEM.

The most complex use of the technique so far was to view two types of
freezer bag, one was failing once frozen! We rolled strips of each bag into
a coil and fractured with support as described. Well, we found that the
bags were made up of layers, 4 in the poor bag 5 in the good bag. The extra
layer, even though you could see through the bag, was aluminium;
interesting?

Hope this helps and by the way we use the straw technique mentioned in cryo
systems only! Guru I may be but clearly in that case I have led people
astray; its age!

Steve

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com

-----Original Message-----
X-from: naomi_mccallum-at-health.qld.gov.au
[mailto:naomi_mccallum-at-health.qld.gov.au]
Sent: 20 February 2009 04:31
To: protrain-at-emcourses.com

Hi all

A tip that I learned from a certain SEM guru was that you get a better
cross-section of the hair shaft if you fracture in liquid nitrogen. He
described this technique of putting the hairs through the centre of a straw
and filling the straw with liquid carbon. After freezing in Liq N2 you hit
the straw with a sharp blade to fracture. There was some discussion about
getting the right type of straw to make this work.

I tried this but didn't persist long enough to master it without bubbles in
the straw, so I modified the technique a little. We use jewellers wire and
crimps to make a 'brush' from multiple hairs (another local technique).
Then we coat the hair in a sucrose blocking media used for histologic frozen
sections; freeze in liq N2; fracture and then wash off the media. It works
well for TS of hair shaft, you get a true fracture and not "a study in
cutting techniques" (I believe that was the description).

We still use the method described below as well, mounting the LS and TS
brush on the same stub.

Regards
Naomi

} } } {john.brealey-at-imvs.sa.gov.au} 20/02/2009 12:06 pm } } }



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi Kristen,
That's a good exercise to do.
It's more interesting when you can be the guinea pig, ie, when you examine
your own hair.
Our method is really basic - we cut the hair with a sharp blade and
carefully place the pieces on a double-sided sticky tab that is stuck to an
SEM stub. Then we coat with gold in a sputter coater.

Regards,

John

Hi John,

I've been following this a bit and am a total novice in examining hair
samples with SEM. I'm always looking for good exercise for my undergrad
students to do with SEM. Would you mind sharing how you prep the samples for
SEM?

Thanks,
Kristen


--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au
{john.brealey-at-imvs.sa.gov.au} wrote:

} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au}
} Subject: [Microscopy] SEM of Hair
} To: kamlennon-at-yahoo.com
} Date: Thursday, February 19, 2009, 7:30 PM
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Hi Naomi,
}
} X-from your question it appears you are more advanced than us when it
} comes to studying hair specimens. We receive one or two hair
} specimens per year so our experience is quite limited.
} We don't follow any diagnostic algorithm and I can't comment on your
} question about telogen effluvium. Maybe someone else here on the
} forum can help.
} We examine the hair shaft and cuticle for structural defects by SEM
} and examine the hair shaft through polarised light by LM.
} Other than that we
} don't have any advanced methodology.
}
} Regards,
}
} John Brealey
} Supervising Scientist
} EM Unit
} Queen Elizabeth Hospital
} SA Pathology
} South Australia
}
}
} Hi John
}
} It was interesting to follow your comments on the Microscopy
} Listserver as we are in a similar position here at Pathology
} Queensland.
} At present out
} pathologist is requesting Whole Mount for polarised LM and then SEM on
} every specimen that walks through the door.
}
} I was curious to know if you follow a diagnostic algorithm for Hair
} specimens. Eg for Telogen Effluvium is it considered necessary to go
} to SEM or just tally ratio of root bulbs at LM?
}
} Thank you for your contributions
} Naomi
}
} Naomi McCallum
} Supervising Scientist
} Electron Microscope Unit
} Anatomical Pathology & Cytopathology
} PATHOLOGY QUEENSLAND
} Central Laboratory
} RBWH
}
}


==============================Original Headers==============================
10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009
10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au
[203.26.121.14])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1K20Ep0027815
10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15
-0600
10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000";
10, 27 -- d="scan'208";a="11464458"
10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au)
([10.9.18.85])
10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009
12:30:14 +1030
10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP
Server id
10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030
10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125])
by
10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for
10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030
(CST)
10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
10, 27 -- Subject: SEM of Hair
10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030
10, 27 -- Organization: IMVS
10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i}
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain; charset="us-ascii"
10, 27 -- Content-Transfer-Encoding: 7bit
10, 27 -- X-Mailer: Microsoft Office Outlook 11
10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g==
==============================End of - Headers==============================

****************************************************************************
****
This email, including any attachments sent with it, is confidential and for
the sole use of the intended recipient(s). This confidentiality is not
waived or lost, if you receive it and you are not the intended recipient(s),
or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this
email is strictly prohibited. The information contained in this email,
including any attachment sent with it, may be subject to a statutory duty of
confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email
in error, you are asked to immediately notify the sender by telephone
collect on Australia +61 1800 198 175 or by return email. You should also
delete this email, and any copies, from your computer system network and
destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or
take any action(s) that relies on it; any form of disclosure, modification,
distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email
does not contain malicious software, Queensland Health does not accept
responsibility for the consequences if any person's computer inadvertently
suffers any disruption to services, loss of information, harm or is infected
with a virus, other malicious computer programme or code that may occur as a
consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender
and not the views of the Queensland Government.
****************************************************************************
******



==============================Original Headers==============================
22, 26 -- From prvs=13026700a6=naomi_mccallum-at-health.qld.gov.au Thu Feb 19
22:29:34 2009
22, 26 -- Received: from gwd-mailedge05.health.qld.gov.au
(smtp3.health.qld.gov.au [165.86.81.114])
22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1K4TWut012703
22, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 22:29:34
-0600
22, 26 -- Received: from gwd-mail06.remote.health.qld.gov.au
(gwd-mail06.remote.health.qld.gov.au [192.168.3.53])
22, 26 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with
ESMTP id n1K4Sfqo004341
22, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:42
+1000
22, 26 -- Received: from health-cs11.health.qld.gov.au (unverified
[10.17.112.31]) by
22, 26 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP
Server)
22, 26 -- with ESMTP id
22, 26 -- {T8ca07f5847c0a80335e00-at-gwd-mail06.remote.health.qld.gov.au}
for
22, 26 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:41 +1000
22, 26 -- Received: from CORPORATE-GWIA01-MTA by
health-cs11.health.qld.gov.au with
22, 26 -- Novell_GroupWise; Fri, 20 Feb 2009 14:28:41 +1000
22, 26 -- Message-Id: {499EBD0F.88BE.00AA.0-at-health.qld.gov.au}
22, 26 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
22, 26 -- Date: Fri, 20 Feb 2009 14:28:36 +1000
22, 26 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au}
22, 26 -- To: {Microscopy-at-microscopy.com}
22, 26 -- Subject: Fwd: [Microscopy] SEM of Hair
22, 26 -- References: {200902200206.n1K26CgH008879-at-ns.microscopy.com}
22, 26 -- Mime-Version: 1.0
22, 26 -- Content-Type: text/plain; charset="us-ascii"
22, 26 -- Content-Disposition: inline
22, 26 -- Content-Transfer-Encoding: 8bit
22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n1K4TWut012703
==============================End of - Headers==============================


==============================Original Headers==============================
37, 27 -- From protrain-at-emcourses.com Fri Feb 20 03:29:09 2009
37, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
37, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K9T8VF002380
37, 27 -- for {microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 03:29:08 -0600
37, 27 -- Received: from 5ac8bf20.bb.sky.com ([90.200.191.32]:4756 helo=HP6220)
37, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
37, 27 -- (envelope-from {protrain-at-emcourses.com} )
37, 27 -- id 1LaRgs-0005Bw-JQ by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 20 Feb 2009 11:29:03 +0200
37, 27 -- Reply-To: {protrain-at-emcourses.com}
37, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com}
37, 27 -- To: {naomi_mccallum-at-health.qld.gov.au}
37, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
37, 27 -- References: {200902200430.n1K4UYTl014110-at-ns.microscopy.com}
37, 27 -- Subject: RE: [Microscopy] Fwd: SEM of Hair
37, 27 -- Date: Fri, 20 Feb 2009 09:28:41 -0000
37, 27 -- Organization: Protrain
37, 27 -- Message-ID: {003901c9933d$ad5b4de0$0200a8c0-at-HP6220}
37, 27 -- MIME-Version: 1.0
37, 27 -- Content-Type: text/plain;
37, 27 -- charset="us-ascii"
37, 27 -- Content-Transfer-Encoding: 7bit
37, 27 -- X-Mailer: Microsoft Office Outlook 11
37, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
37, 27 -- Thread-Index: AcmTFAWjDrrRW1vERqyTtsCF/XOxsAAJgpIg
37, 27 -- In-Reply-To: {200902200430.n1K4UYTl014110-at-ns.microscopy.com}
37, 27 -- X-Scan-Signature: e61ed4b52b61ed9601f755c09be3f9a3{293}}
37, 27 -- X-Trace: smtp01.dial-up.net 1LaRgs-0005Bw-JQ 6cb117f5a30fc6b35b332f22f9d3c1ea
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 20 Feb 2009 07:47:58 -0600
Subject: [Microscopy] RE: Fwd: SEM of Hair : carbon solution ??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve, Naomi

Maybe a awkward question, but I'm not chemist : what do you call "a
water soluble carbon solution" or "liquid carbon" ?

Do you think at carbon paint, something like Leit C or a suspension of
carbon black in water ?

Jacques

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
7, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Feb 20 07:47:57 2009
7, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KDluXN026656
7, 29 -- for {microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 07:47:57 -0600
7, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
7, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n1KDlr5K038068
7, 29 -- for {microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:47:54 +0100 (CET)
7, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
7, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
7, 29 -- (No client certificate requested)
7, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 1457A3EC002
7, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Feb 2009 14:47:46 +0100 (CET)
7, 29 -- Message-ID: {499EB482.6050707-at-ipcms.u-strasbg.fr}
7, 29 -- Date: Fri, 20 Feb 2009 14:47:46 +0100
7, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
7, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
7, 29 -- MIME-Version: 1.0
7, 29 -- To: Microscopy-at-microscopy.com
7, 29 -- Subject: RE: Fwd: SEM of Hair : carbon solution ??
7, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-IPCMS-MailScanner: Found to be clean
7, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
7, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 20 Feb 2009 14:47:54 +0100 (CET)
7, 29 -- X-Virus-Scanned: ClamAV 0.94.2/9016/Fri Feb 20 05:03:22 2009 on mr3.u-strasbg.fr
7, 29 -- X-Virus-Status: Clean
7, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
7, 29 -- autolearn=disabled version=3.2.5
7, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr3.u-strasbg.fr
==============================End of - Headers==============================




From: gul417-at-mail.usask.ca
Date: Fri, 20 Feb 2009 10:17:07 -0600
Subject: [Microscopy] TEM of fossil tooth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I have a researcher who will be wanting to cut ultrathin sections of
fossil tooth pieces (size ~ 3x5mm) for TEM observation.

Since most of my TEM experience come from plant materials, I'm
wondering if there are some special requirement/tricks for sample
preparation (e.g. embedding), microtome (knife selection, cryo or RT)
and post-staining etc.

I googled the internet but could not find much info/protocols to
follow. Any suggestion and advice are greatly appreciated.

Thanks in advance.

Guosheng
Dept of Biology
U of Saskatchewan
Canada

==============================Original Headers==============================
6, 20 -- From gul417-at-mail.usask.ca Fri Feb 20 10:17:05 2009
6, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KGH2Sc014574
6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Feb 2009 10:17:04 -0600
6, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
6, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
6, 20 -- id {0KFD00C01GXVCQ-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
6, 20 -- for Microscopy-at-Microscopy.Com; Fri, 20 Feb 2009 10:16:16 -0600 (CST)
6, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31])
6, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
6, 20 -- with SMTP id {0KFD00KGRH7479-at-usask.ca} for Microscopy-at-Microscopy.Com; Fri,
6, 20 -- 20 Feb 2009 10:16:16 -0600 (CST)
6, 20 -- Date: Fri, 20 Feb 2009 10:16:16 -0600 (CST)
6, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
6, 20 -- Subject: TEM of fossil tooth
6, 20 -- To: Microscopy-at-Microscopy.Com
6, 20 -- Message-id: {2208155.1235146576267.JavaMail.gul417-at-mail.usask.ca}
6, 20 -- MIME-version: 1.0
6, 20 -- Content-type: text/plain; charset=UTF-8
6, 20 -- Content-transfer-encoding: 7BIT
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Fri, 20 Feb 2009 14:28:39 -0600
Subject: [Microscopy] TEM of fossil tooth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Use a FIB with lift-out. Especially if the fossils are valuable and you
don't want to consume the tooth in making a TEM sample.

John Mardinly,
Numonyx

-----Original Message-----
X-from: gul417-at-mail.usask.ca [mailto:gul417-at-mail.usask.ca]
Sent: Friday, February 20, 2009 8:37 AM
To: MARDINLY, A

Dear All,

I have a researcher who will be wanting to cut ultrathin sections of
fossil tooth pieces (size ~ 3x5mm) for TEM observation.

Since most of my TEM experience come from plant materials, I'm
wondering if there are some special requirement/tricks for sample
preparation (e.g. embedding), microtome (knife selection, cryo or RT)
and post-staining etc.

I googled the internet but could not find much info/protocols to
follow. Any suggestion and advice are greatly appreciated.

Thanks in advance.

Guosheng
Dept of Biology
U of Saskatchewan
Canada

==============================Original
Headers==============================
6, 20 -- From gul417-at-mail.usask.ca Fri Feb 20 10:17:05 2009
6, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n1KGH2Sc014574
6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Feb 2009
10:17:04 -0600
6, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
6, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
6, 20 -- id {0KFD00C01GXVCQ-at-usask.ca} (original mail from
gul417-at-mail.usask.ca)
6, 20 -- for Microscopy-at-Microscopy.Com; Fri, 20 Feb 2009 10:16:16 -0600
(CST)
6, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31])
6, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built
Jul 3 2007))
6, 20 -- with SMTP id {0KFD00KGRH7479-at-usask.ca} for
Microscopy-at-Microscopy.Com; Fri,
6, 20 -- 20 Feb 2009 10:16:16 -0600 (CST)
6, 20 -- Date: Fri, 20 Feb 2009 10:16:16 -0600 (CST)
6, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
6, 20 -- Subject: TEM of fossil tooth
6, 20 -- To: Microscopy-at-Microscopy.Com
6, 20 -- Message-id:
{2208155.1235146576267.JavaMail.gul417-at-mail.usask.ca}
6, 20 -- MIME-version: 1.0
6, 20 -- Content-type: text/plain; charset=UTF-8
6, 20 -- Content-transfer-encoding: 7BIT
==============================End of -
Headers==============================



==============================Original Headers==============================
15, 29 -- From A.MARDINLY-at-numonyx.com Fri Feb 20 14:28:38 2009
15, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38])
15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KKScgY006822
15, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 20 Feb 2009 14:28:38 -0600
15, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
15, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id A682624801A;
15, 29 -- Fri, 20 Feb 2009 14:50:28 -0500 (EST)
15, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
15, 29 -- Fri, 20 Feb 2009 15:28:37 -0500
15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
15, 29 -- Content-class: urn:content-classes:message
15, 29 -- MIME-Version: 1.0
15, 29 -- Content-Type: text/plain;
15, 29 -- charset="us-ascii"
15, 29 -- Subject: RE: [Microscopy] TEM of fossil tooth
15, 29 -- Date: Fri, 20 Feb 2009 15:28:09 -0500
15, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9E19622-at-EXDRESBENMX012.numonyx.local}
15, 29 -- In-Reply-To: {200902201636.n1KGaiIM026897-at-ns.microscopy.com}
15, 29 -- X-MS-Has-Attach:
15, 29 -- X-MS-TNEF-Correlator:
15, 29 -- Thread-Topic: [Microscopy] TEM of fossil tooth
15, 29 -- Thread-Index: AcmTeXWAbT4oTPQ/S4GosfWMMsXn5AAIBIDg
15, 29 -- References: {200902201636.n1KGaiIM026897-at-ns.microscopy.com}
15, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
15, 29 -- To: {gul417-at-mail.usask.ca}
15, 29 -- Cc: {Microscopy-at-Microscopy.com}
15, 29 -- X-OriginalArrivalTime: 20 Feb 2009 20:28:37.0884 (UTC) FILETIME=[CF972BC0:01C99399]
15, 29 -- Content-Transfer-Encoding: 8bit
15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1KKScgY006822
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Sat, 21 Feb 2009 15:48:21 -0600
Subject: [Microscopy] Looking for manual & parts...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all, I just got an ISI ABT-55, and while the manual doesn't look
too different from the SX40 I've got, it looks different enough to
warrant getting an English copy. You see, the manual I have is
entirely in Japanese. I will, of course, offer the Japanese version
as trade for a copy of an English version... The perfect gift for
anyone looking to learn another language!

Also, if anybody has one of these that is mothballed or headed that
way, please contact me off list, I am in need of a few ABT-55 specific
odds and ends.

Thanks,

Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
5, 31 -- From kraftpiano-at-gmail.com Sat Feb 21 15:48:20 2009
5, 31 -- Received: from mail-qy0-f20.google.com (mail-qy0-f20.google.com [209.85.221.20])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1LLmKWl018696
5, 31 -- for {microscopy-at-microscopy.com} ; Sat, 21 Feb 2009 15:48:20 -0600
5, 31 -- Received: by qyk13 with SMTP id 13so2333571qyk.18
5, 31 -- for {microscopy-at-microscopy.com} ; Sat, 21 Feb 2009 13:48:20 -0800 (PST)
5, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
5, 31 -- :from:to:content-type:content-transfer-encoding;
5, 31 -- bh=CjJPGi0F2aPEDlVLb952S0xu74Zlf2uvALyf04bxtdw=;
5, 31 -- b=g3hX3iFonGU4NL1PKbWaFcquQ6xOe2uEVBZqU7DW8J24PLNMEGOT6bzcEH/yn951p/
5, 31 -- LRTygztVPcea9sgSNVKmJxYN85lSwO+/LHg7AjclK5T7+dtB+QlrWmrLzU+VuktWp49T
5, 31 -- EQ4+zTYhG8QWPYnkBdcm/2HvB4VjA5vqM2UVU=
5, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
5, 31 -- :content-transfer-encoding;
5, 31 -- b=xUaZBJtQWSXrH+Tc34DmPTpqAtQoRotJLH1eSjP1PIpnjc+Jbmnktq5Ia64OBlLEEx
5, 31 -- OUB4YNQlJM1JPzmAG1aZOwQh6ATNsO7QoVvRBxAhtc9hmZNW+MUwfNCuDCfTkHe7R9Sb
5, 31 -- XD9VFvyecJRlIveHStDOc9xZFKb4VRgkQKFAs=
5, 31 -- MIME-Version: 1.0
5, 31 -- Received: by 10.224.37.17 with SMTP id v17mr3699273qad.128.1235252900403; Sat,
5, 31 -- 21 Feb 2009 13:48:20 -0800 (PST)
5, 31 -- Date: Sat, 21 Feb 2009 16:48:19 -0500
5, 31 -- Message-ID: {25e2b0d20902211348k27dd23a5mf8b3d39f80cccd6c-at-mail.gmail.com}
5, 31 -- Subject: Looking for manual & parts...
5, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
5, 31 -- To: microscopy-at-microscopy.com
5, 31 -- Content-Type: text/plain; charset=ISO-8859-1
5, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: slc6-at-lehigh.edu
Date: Mon, 23 Feb 2009 14:53:51 -0600
Subject: [Microscopy] viaWWW: Lehigh Microscopy School Courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both slc6-at-lehigh.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: slc6-at-lehigh.edu
Name: Sharon Coe

Organization: Lehigh Microscopy School

Title-Subject: [Filtered] Lehigh Microscopy School Courses

Question: There is still time to register for the 2009 Lehigh
Microscopy School which will be held May 31-June 12, 2009. This will
be the 39th year of course offerings which include: SEM and X-ray
Microanalysis (May 31-June 5), Introduction to SEM and EDS for the
New Operator (May 31), Scanning Probe Microscopy: From Fundamentals
to Advanced Applications (June 8-11), Problem Solving with SEM, X-ray
Microanalysis, and Electron Backscatter Patterns (June 8-12),
Quantitative X-ray Microanalysis: Problem Solving using EDS and WDS
Techniques (June 8-12), Scanning Transmission Electron Microscopy:
From Fundamentals to Advanced Applications (June 8-11), Focused Ion
Beam (FIB) Instrumentation and Applications (June 8-11). Complete
course descriptions and registration form are available at
www.Lehigh.edu/microscopy. Contact Sharon Coe
(Sharon.coe-at-Lehigh.edu) for more information.

Login Host: 128.180.55.124
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Feb 23 14:53:51 2009
6, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1NKrf9O025757
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Feb 2009 14:53:49 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240838c5c8bd407fb0-at-[130.194.133.34]}
6, 11 -- Date: Tue, 24 Feb 2009 07:53:32 +1100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: slc6-at-lehigh.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Lehigh Microscopy School Courses
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: smythen-at-musc.edu
Date: Mon, 23 Feb 2009 14:54:07 -0600
Subject: [Microscopy] viaWWW: 3d reconstruction software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both smythen-at-musc.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: smythen-at-musc.edu
Name: Nancy Smythe

Organization: Medical University of South Carolina

Title-Subject: [Filtered] 3d reconstruction software

Question: Is there a software that most people are using to
reconstruct serial sections both at the em and lm levels? I used on
once maybe 10 years ago but don't remember who developed it.

Login Host: 128.23.149.163
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Feb 23 14:54:07 2009
6, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1NKs1J0025834
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Feb 2009 14:54:05 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240839c5c8bd5283b7-at-[130.194.133.34]}
6, 11 -- Date: Tue, 24 Feb 2009 07:53:52 +1100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: smythen-at-musc.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: 3d reconstruction software
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: srijay-at-stanford.edu
Date: Mon, 23 Feb 2009 16:19:53 -0600
Subject: [Microscopy] viaWWW: processing nerve cells on ACLAR for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both srijay-at-stanford.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: srijay-at-stanford.edu
Name: Jay

Organization: Stanford University

Title-Subject: [Filtered] processing nerve cells on ACLAR for TEM

Question: Hi,

I have a couple of questions-

1. What is the best way to sterilise Aclar discs to grow nerve cells.

2. After infiltration step how can the discs be embedded for thin sectioning?

I appreciate your input!

thanks,
Jay

Login Host: 171.65.115.77
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Mon Feb 23 16:19:52 2009
11, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1NMJlDA022959
11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Feb 2009 16:19:50 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p0624083bc5c8d16a3982-at-[130.194.133.34]}
11, 11 -- Date: Tue, 24 Feb 2009 09:19:39 +1100
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: srijay-at-stanford.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: processing nerve cells on ACLAR for TEM
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Mon, 23 Feb 2009 17:09:30 -0600
Subject: [Microscopy] viaWWW: Tannic Acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Tannic Acid

Question: Is it possible that tannic acid - the powder itself - in a
plastic jar, can expire?

We are having some issues with getting good contrast. I have been
following a published protocol for imaging cilia in the kidneys of
zebrafish.

Just trying to systematically see if there is something different
that we are doing and wondering if it could be that bottle of tannic
acid on the shelf.

Thanks, Peggy

Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Feb 23 17:09:30 2009
9, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1NN9GWG005605
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Feb 2009 17:09:20 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p0624083dc5c8dcfbef74-at-[130.194.133.34]}
9, 11 -- Date: Tue, 24 Feb 2009 10:09:07 +1100
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Tannic Acid
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Mon, 23 Feb 2009 17:41:41 -0600
Subject: [Microscopy] viaWWW: Tannic Acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The type of tannic acid makes a big difference. There are low and high
molecular weight forms depending on its source. Go the EMS website and
the technical data sheet for their tannic acid has a nice description of
this issue and literature citations. (No commercial interest - just a
happy user). Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: mbisher-at-princeton.edu [mailto:mbisher-at-princeton.edu]
Sent: Monday, February 23, 2009 5:11 PM
To: Phillips, Thomas E.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Tannic Acid

Question: Is it possible that tannic acid - the powder itself - in a
plastic jar, can expire?

We are having some issues with getting good contrast. I have been
following a published protocol for imaging cilia in the kidneys of
zebrafish.

Just trying to systematically see if there is something different
that we are doing and wondering if it could be that bottle of tannic
acid on the shelf.

Thanks, Peggy

Login Host: 128.112.160.214
------------------------------------------------------------------------
---

==============================Original
Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Feb 23 17:09:30 2009
9, 11 -- Received: from [130.194.133.34] (msdvpn072.msd.anl.gov
[130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n1NN9GWG005605
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Feb 2009
17:09:20 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p0624083dc5c8dcfbef74-at-[130.194.133.34]}
9, 11 -- Date: Tue, 24 Feb 2009 10:09:07 +1100
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Tannic Acid
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 29 -- From PhillipsT-at-missouri.edu Mon Feb 23 17:41:41 2009
20, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1NNfdMf020033
20, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2009 17:41:40 -0600
20, 29 -- X-IronPort-Anti-Spam-Filtered: true
20, 29 -- X-IronPort-Anti-Spam-Result: ApoEAGPCoknRauUo/2dsb2JhbADIFQEBAQeFGIhKAYJfAQGBLwaGKw
20, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
20, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 23 Feb 2009 17:41:35 -0600
20, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
20, 29 -- Mon, 23 Feb 2009 17:41:35 -0600
20, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 29 -- Content-class: urn:content-classes:message
20, 29 -- MIME-Version: 1.0
20, 29 -- Content-Type: text/plain;
20, 29 -- charset="us-ascii"
20, 29 -- Subject: RE: [Microscopy] viaWWW: Tannic Acid
20, 29 -- Date: Mon, 23 Feb 2009 17:41:34 -0600
20, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD0604D2FD-at-UM-XMAIL06.um.umsystem.edu}
20, 29 -- In-Reply-To: {200902232310.n1NNAU70006720-at-ns.microscopy.com}
20, 29 -- X-MS-Has-Attach:
20, 29 -- X-MS-TNEF-Correlator:
20, 29 -- Thread-Topic: [Microscopy] viaWWW: Tannic Acid
20, 29 -- Thread-Index: AcmWC/ARSbp1krsORJaNUlaIupW9IwAA92GQ
20, 29 -- References: {200902232310.n1NNAU70006720-at-ns.microscopy.com}
20, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
20, 29 -- To: {mbisher-at-princeton.edu} , {Microscopy-at-microscopy.com}
20, 29 -- X-OriginalArrivalTime: 23 Feb 2009 23:41:35.0963 (UTC) FILETIME=[43E712B0:01C99610]
20, 29 -- Content-Transfer-Encoding: 8bit
20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1NNfdMf020033
==============================End of - Headers==============================




From: mzemyan-at-schaferlabs.com
Date: Tue, 24 Feb 2009 13:02:52 -0600
Subject: [Microscopy] SIMS engineer/scientist opening at Schafer Corporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Announcement of Senior Engineer/Scientist opening at Schafer Corporation.

Schafer Vallecitos Laboratory is seeking a Senior Engineer / Scientist to
add to our mass spectrometry group. SVL performs materials characterization
and related analytical services on commercial and government contracts. The
activities of the group include chemical and elemental analysis of materials
on a production basis, maintenance of several mass spectrometers and
ancillary equipment, development of new or improved MS analysis techniques,
and quality assurance of analytical data.

Responsibilities:
The Senior Engineer / Scientist will provide theoretical and practical
knowledge in the design, operation and maintenance of secondary ion mass
spectrometry (SIMS) instruments and analysis of the data they produce.


For details on this opening, see http://jobs-schafer.icims.com/jobs/1515/job
(Do not reply to this message.)



==============================Original Headers==============================
5, 19 -- From mzemyan-at-schaferlabs.com Tue Feb 24 13:02:52 2009
5, 19 -- Received: from mail.schaferlabs.com (mx1.schaferlabs.com [64.168.91.154])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1OJ2nEI004055
5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 13:02:51 -0600
5, 19 -- Received: from mzemyanws ([10.10.10.1])
5, 19 -- by mail.schaferlabs.com (8.12.11.20060308/8.12.11) with ESMTP id n1OJ2lUp027339
5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 11:02:47 -0800
5, 19 -- Message-Id: {200902241902.n1OJ2lUp027339-at-mail.schaferlabs.com}
5, 19 -- From: "Mike Zemyan" {mzemyan-at-schaferlabs.com}
5, 19 -- To: {Microscopy-at-microscopy.com}
5, 19 -- Subject: SIMS engineer/scientist opening at Schafer Corporation
5, 19 -- Date: Tue, 24 Feb 2009 11:02:48 -0800
5, 19 -- MIME-Version: 1.0
5, 19 -- Content-Type: text/plain;
5, 19 -- charset="us-ascii"
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353
5, 19 -- thread-index: AcmWsnwjAXc2sjAQRYSuluTi3XIkZw==
5, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Tue, 24 Feb 2009 16:03:16 -0600
Subject: [Microscopy] Cleaning aperture strip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a number of very pricey aperture strips that need cleaned. The best
way would be with a plasma cleaner. Does anyone have one who would be
willing to try to clean a strip or can you recommend somewhere that I could
send the strips for cleaning?

Debby

--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


==============================Original Headers==============================
5, 29 -- From dsherman-at-purdue.edu Tue Feb 24 16:03:16 2009
5, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131])
5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1OM3FhK023718
5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:03:15 -0600
5, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
5, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1OM3FOG011542
5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 17:03:15 -0500
5, 29 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11])
5, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1OM3FFo025403
5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 17:03:15 -0500
5, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
5, 29 -- Tue, 24 Feb 2009 17:03:14 -0500
5, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ;
5, 29 -- Tue, 24 Feb 2009 22:02:42 +0000
5, 29 -- User-Agent: Microsoft-Entourage/12.15.0.081119
5, 29 -- Date: Tue, 24 Feb 2009 17:02:40 -0500
5, 29 -- Subject: Cleaning aperture strip
5, 29 -- From: Debby Sherman {dsherman-at-purdue.edu}
5, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
5, 29 -- Message-ID: {C5C9D8B0.3B1D1%dsherman-at-exchange.purdue.edu}
5, 29 -- Thread-Topic: Cleaning aperture strip
5, 29 -- Thread-Index: AcmWy5w0YbLFc2iDQwmDVw4N2g/D8Q==
5, 29 -- Mime-version: 1.0
5, 29 -- Content-type: text/plain;
5, 29 -- charset="US-ASCII"
5, 29 -- Content-transfer-encoding: 7bit
5, 29 -- X-OriginalArrivalTime: 24 Feb 2009 22:03:14.0670 (UTC) FILETIME=[B0DEF8E0:01C996CB]
5, 29 -- X-PMX-Version: 5.4.0.320885
5, 29 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 24 Feb 2009 16:03:52 -0600
Subject: [Microscopy] Temporary Clinical Electron Microscopy Job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

Anyone interested in a temporary clinical TEM job?

Reply to April, see e-mail below.

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Tue, 2/24/09, April Sachau {asachau-at-titanmed.com} wrote:

} From: April Sachau {asachau-at-titanmed.com}
} Subject: RE: [Histonet] Electron Microscopy
} To: vapatpxs-at-yahoo.com
} Date: Tuesday, February 24, 2009, 8:38 PM
} I am working with a hospital here in Nebraska, and their EM
} histo tech
} has just left for an emergency maternity leave. They need
} some full
} time coverage while she is gone. Do you know of anyone
} that might be
} willing to work out of town for 3-4 months??? I would be
} able to pay
} for housing, travel...etc along with a competitive salary.
} I appreciate
} your help! (Also, I have been approved to give anyone
} referring a
} qualified individual a $250.00 referral fee!)
}
}
} April Sachau
} Titan Medical Group
} Staff Supervisor
} Phone (866) 332-9600 Ext. 1023
} Fax (402) 332-5181
} asachau-at-titanmed.com
}
} see us on the web at www.titanmed.com
}
} -----Original Message-----
} From: Va Paula Sicurello [mailto:vapatpxs-at-yahoo.com]
} Sent: Tuesday, February 24, 2009 11:44 AM
} To: April Sachau
} Subject: Re: [Histonet] Electron Microscopy
}
} Hi April,
}
} I have extensive experience with TEM and SEM.
}
} You can call me at the number below.
}
} Paula Sicurello
} VA Medical Center San Diego
} Veterans Medical Research Foundation (VMRF)
} Core Microscope Facility, room B141
} 3350 La Jolla Village Dr., MC151
} San Diego, CA 92161
} 858-552-8585 x2397
}
}
} --- On Tue, 2/24/09, April Sachau
} {asachau-at-titanmed.com} wrote:
}
} } From: April Sachau {asachau-at-titanmed.com}
} } Subject: [Histonet] Electron Microscopy
} } To: histonet-at-lists.utsouthwestern.edu
} } Date: Tuesday, February 24, 2009, 5:04 PM
} } Hello Histoland!
} }
} } I am seeking an individual with Electron Microscopy
} } experience... please
} } contact me if you have it or know of someone that
} does.
} } Thanks!
} }
} }
} } April Sachau
} } Titan Medical Group
} } Staff Supervisor
} } Phone (866) 332-9600 Ext. 1023
} } Fax (402) 332-5181
} } asachau-at-titanmed.com
} }
} } see us on the web at www.titanmed.com
} }
} } _______________________________________________
} } Histonet mailing list
} } Histonet-at-lists.utsouthwestern.edu
} }
} http://lists.utsouthwestern.edu/mailman/listinfo/histonet





==============================Original Headers==============================
10, 26 -- From vapatpxs-at-yahoo.com Tue Feb 24 16:03:51 2009
10, 26 -- Received: from n67.bullet.mail.sp1.yahoo.com (n67.bullet.mail.sp1.yahoo.com [98.136.44.47])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1OM3pZh024205
10, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:03:51 -0600
10, 26 -- Received: from [69.147.84.145] by n67.bullet.mail.sp1.yahoo.com with NNFMP; 24 Feb 2009 21:55:00 -0000
10, 26 -- Received: from [68.142.194.243] by t8.bullet.mail.sp1.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000
10, 26 -- Received: from [68.142.201.68] by t1.bullet.mud.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000
10, 26 -- Received: from [127.0.0.1] by omp420.mail.mud.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000
10, 26 -- X-Yahoo-Newman-Property: ymail-3
10, 26 -- X-Yahoo-Newman-Id: 781934.61652.bm-at-omp420.mail.mud.yahoo.com
10, 26 -- Received: (qmail 44816 invoked by uid 60001); 24 Feb 2009 21:54:59 -0000
10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 26 -- s=s1024; d=yahoo.com;
10, 26 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID;
10, 26 -- b=o3kFtHPrePKfshrsTbTurONZNb4/0/Pk8uROC2UIIfz+s+lKxGB4BxcIhnchSPjswQt2maimYvoTxxC+qyQrlvFVU316IzWTEqMXhTIKVXmH7dEogtUE2ze0yJddOhiAHS+CN2B2uhepnRyz1TC2v/QhwIoKM8jsIQ4LQVaT970=;
10, 26 -- X-YMail-OSG: 4Q.NNHYVM1lT46sNf3CHX8GpgGcG363lFkk0v48qPCyl8Yv.yMBxyOcGM9j2snc5a43DlGYfe3Yn3vIeBkN09KMDuh1UytYd9OQhoBUVpJ2zImjPfu4tgafzynDHj5VTEhK6hfU8Aw40CDxiOVfsVtg6V9WbTl89a0_nqeC6zE2UC_E22Ok65C0mUqm6dl3Gxc.jZmFB6boEkvc-
10, 26 -- Received: from [132.239.85.26] by web46114.mail.sp1.yahoo.com via HTTP; Tue, 24 Feb 2009 13:54:59 PST
10, 26 -- X-Mailer: YahooMailWebService/0.7.260.1
10, 26 -- Date: Tue, 24 Feb 2009 13:54:59 -0800 (PST)
10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
10, 26 -- Reply-To: vapatpxs-at-yahoo.com
10, 26 -- Subject: Temporary Clinical Electron Microscopy Job
10, 26 -- To: MSA BB {Microscopy-at-microscopy.com}
10, 26 -- MIME-Version: 1.0
10, 26 -- Content-Type: text/plain; charset=us-ascii
10, 26 -- Message-ID: {248525.44422.qm-at-web46114.mail.sp1.yahoo.com}
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Tue, 24 Feb 2009 16:32:55 -0600
Subject: [Microscopy] AFM: recommendations for imaging wet DNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for recommendations for AFM systems to image wet DNA and
other bio-polymers.

We are looking for a complete system. Experienced users and vendors
are welcome to respond with particulars (capabilities, pricing,
advantages over competiting systems, etc.)

Thanks,
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
5, 19 -- From bozzola-at-siu.edu Tue Feb 24 16:32:55 2009
5, 19 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1OMWqL6019153
5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:32:54 -0600
5, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
5, 19 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n1OMWka4000140
5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:32:49 -0600 (CST)
5, 19 -- Mime-Version: 1.0
5, 19 -- Message-Id: {a0624080dc5ca24b87e53-at-[131.230.177.136]}
5, 19 -- In-Reply-To: {200902242204.n1OM4fre026401-at-ns.microscopy.com}
5, 19 -- References: {200902242204.n1OM4fre026401-at-ns.microscopy.com}
5, 19 -- Date: Tue, 24 Feb 2009 16:32:46 -0600
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
5, 19 -- Subject: AFM: recommendations for imaging wet DNA
5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 19 -- X-Spam-Score: 0.00%
5, 19 -- X-MASF: 0.00%
5, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: abrun-at-hsc.unt.edu
Date: Tue, 24 Feb 2009 21:27:32 -0600
Subject: [Microscopy] viaWWW: Epon/PAS stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abrun-at-hsc.unt.edu
Name: Anne-Marie Brun

Organization: UNT HSC at Fort Worth Texas 76107, USA

Title-Subject: [Filtered] Epon/PAS stain

Question: Has anyone ever stained for PAS on Epon sections before? If
not on Epon then what type of plastic did you use to do a PAS stain?
I know it is done on paraffin embedded material, but an investigator
wants the PAS staining done on Epon sections. ----?
Thanks for your help
Anne-Marie

Login Host: 129.120.96.228
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:32 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3RVv3007908
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:31 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5ca6b1378b7-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:29 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Epon/PAS stain
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: analytic-at-rawbw.com
Date: Tue, 24 Feb 2009 21:27:59 -0600
Subject: [Microscopy] viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both analytic-at-rawbw.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: analytic-at-rawbw.com
Name: Margo Gill-Linscott

Organization: Analyticus, Inc.

Title-Subject: [Filtered] broken window on EDX detector

Question: I understand that once a 'window' on an EDX detector is
broken there is no way to repair it and the crystal is destroyed. Is
this true and does this apply to all types of detectors?

Login Host: 198.144.223.152
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:58 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3Rt9m008169
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:58 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c5ca6b297dc1-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:55 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: broken window on EDX detector
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 24 Feb 2009 22:58:40 -0600
Subject: [Microscopy] Re: viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Uh...yes. If the window is fractured, then you
are SOL. This means that you need to get the
detector repaired. This is not an insignificant
cost. But it makes the difference working and
not working.

Why did the window fail? This usually happens
if the chamber is vented too quickly or for some
other actions that are detrimental to the window.

The "standard" windows are MoxD and are .3u thick.
These are classified as SUTW (Super Ultra Thin Window)
and are sourced from UT.

I would strongly suggest that you look into why
the window/film failed. You must not repeat this
scenario.

gary g.



At 07:31 PM 2/24/2009, you wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Tue Feb 24 22:58:39 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1P4wbfi004513
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 22:58:38 -0600
9, 20 -- Message-Id: {200902250458.n1P4wbfi004513-at-ns.microscopy.com}
9, 20 -- Received: (qmail 15402 invoked from network); 24 Feb 2009 20:56:59 -0800
9, 20 -- Received: by simscan 1.1.0 ppid: 15395, pid: 15398, t: 0.1083s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 24 Feb 2009 20:56:59 -0800
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Tue, 24 Feb 2009 20:58:30 -0800
9, 20 -- To: analytic-at-rawbw.com
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: broken window on EDX detector
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200902250331.n1P3V9uG012757-at-ns.microscopy.com}
9, 20 -- References: {200902250331.n1P3V9uG012757-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: steve.parry-at-uwa.edu.au
Date: Tue, 24 Feb 2009 23:32:30 -0600
Subject: [Microscopy] SEM - XL30 computer problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We are having problems with our XL30, where the screen freezes and
turns red! Has anyone had similar problems and found what caused it???
We think it is the graphics card, which after re-seating it, the
problem will go away for about a week, then it returns!
We have been told we can get a new card for AUD 15,000, but thought I
would see if anyone had any other ideas first. Does anyone have an
old XL30 computer they could let us have the cards out of??

Thanks

Steve Parry
Centre for Microscopy, Characterisation & Analysis, (M010)
The University of Western Australia,
35 Stirling Highway, Crawley,
WA 6009, Australia.

Phone: +61-8-6488-8057 [24 hour voicemail attached]
Fax: +61-8-6488 -1087
Email: steve.parry-at-uwa.edu.au
http://cmca.uwa.edu.au/



==============================Original Headers==============================
7, 31 -- From steve.parry-at-uwa.edu.au Tue Feb 24 23:32:29 2009
7, 31 -- Received: from mailext2.its.uwa.edu.au (mailext2.its.uwa.edu.au [130.95.128.60])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P5WSHQ018918
7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 23:32:28 -0600
7, 31 -- Received: from kas30pipe.localhost (localhost.localdomain [127.0.0.1])
7, 31 -- by panacea.uwa.edu.au (Postfix) with ESMTP id 32DD327E13
7, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 14:32:25 +0900 (WST)
7, 31 -- Received: from panacea (localhost.localdomain [127.0.0.1])
7, 31 -- by panacea.prekas (Postfix) with SMTP id 1675527E23
7, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 14:32:24 +0900 (WST)
7, 31 -- X-UWA-Client-IP: 130.95.124.138 (UWA)
7, 31 -- Received: from [130.95.124.138] (unknown [130.95.124.138])
7, 31 -- by panacea.input (Postfix) with ESMTP id 0157427E21
7, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 14:32:24 +0900 (WST)
7, 31 -- Mime-Version: 1.0 (Apple Message framework v753.1)
7, 31 -- Content-Transfer-Encoding: 7bit
7, 31 -- Message-Id: {25607907-E79E-4025-B3AB-D33CF6AA38FE-at-uwa.edu.au}
7, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
7, 31 -- To: Microscopy-at-microscopy.com
7, 31 -- From: Steve Parry {steve.parry-at-uwa.edu.au}
7, 31 -- Subject: SEM - XL30 computer problems
7, 31 -- Date: Wed, 25 Feb 2009 14:32:24 +0900
7, 31 -- X-Mailer: Apple Mail (2.753.1)
7, 31 -- X-SpamTest-Envelope-From: steve.parry-at-uwa.edu.au
7, 31 -- X-SpamTest-Group-ID: 00000000
7, 31 -- X-SpamTest-Info: Profiles 7470 [Feb 25 2009]
7, 31 -- X-SpamTest-Method: none
7, 31 -- X-SpamTest-Rate: 0
7, 31 -- X-SpamTest-Status: Not detected
7, 31 -- X-SpamTest-Status-Extended: not_detected
7, 31 -- X-SpamTest-Version: SMTP-Filter Version 3.0.0 [0278], KAS30/Release
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 24 Feb 2009 23:57:07 -0600
Subject: [Microscopy] Re: SEM - XL30 computer problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What???? Why not replace the video card? I
assume that you do not have a maintenance contract.

If you are using or stuck at WinNT, it is crappy.
What are you running for the OS?

gary g.



At 09:34 PM 2/24/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 20 -- From gary-at-gaugler.com Tue Feb 24 23:57:07 2009
11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1P5v5m5000606
11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 23:57:06 -0600
11, 20 -- Message-Id: {200902250557.n1P5v5m5000606-at-ns.microscopy.com}
11, 20 -- Received: (qmail 6254 invoked from network); 24 Feb 2009 22:00:36 -0800
11, 20 -- Received: by simscan 1.1.0 ppid: 6251, pid: 6252, t: 0.1035s
11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 20 -- by smtp1 with SMTP; 24 Feb 2009 22:00:36 -0800
11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 20 -- Date: Tue, 24 Feb 2009 21:57:00 -0800
11, 20 -- To: steve.parry-at-uwa.edu.au
11, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 20 -- Subject: Re: [Microscopy] SEM - XL30 computer problems
11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 20 -- In-Reply-To: {200902250534.n1P5Yp7i021319-at-ns.microscopy.com}
11, 20 -- References: {200902250534.n1P5Yp7i021319-at-ns.microscopy.com}
11, 20 -- Mime-Version: 1.0
11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Wed, 25 Feb 2009 00:14:08 -0600
Subject: [Microscopy] viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The EDS cannot operate with a broken light element window and the EDS will
have to be sent back for repairs to the original vendor or a detector repair
company. The latter is usually less expensive. This might run from $4K -
10K. I am familiar with PulsTor / ATT and also possibly Sematech Systems
might do this or know of someone. There may be others, as well. Here's some
contact info:

PulseTor / AAT
Jim Nicolino
1816 St. Johns Bluff Road
Suite 305
Jacksonville, FL 32246
(904)646-3069
Jim Nicolino [JNicolino-at-comcast.net]

SEMTech Solutions, Inc.
6 Executive Park Drive
N. Billerica, MA 01862
Mark Reynolds
[mreynolds-at-semtechsolutions.com]
www.sts-elionix.com
Tel: (978) 663-9822 x235
Cell: (978) 828-7648
Fax: (978) 663-9823

I hope that helps.



Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.



Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: analytic-at-rawbw.com [mailto:analytic-at-rawbw.com]
Sent: Tuesday, February 24, 2009 7:44 PM
To: dkloos-at-parallaxray.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both analytic-at-rawbw.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: analytic-at-rawbw.com
Name: Margo Gill-Linscott

Organization: Analyticus, Inc.

Title-Subject: [Filtered] broken window on EDX detector

Question: I understand that once a 'window' on an EDX detector is
broken there is no way to repair it and the crystal is destroyed. Is
this true and does this apply to all types of detectors?

Login Host: 198.144.223.152
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:58 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1P3Rt9m008169
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:58
-0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c5ca6b297dc1-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:55 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: broken window on EDX detector
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
19, 30 -- From dkloos-at-parallaxray.com Wed Feb 25 00:14:07 2009
19, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P6E5mr014522
19, 30 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 00:14:06 -0600
19, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
19, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
19, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
19, 30 -- id 1LcD1v-000Jeo-GR; Tue, 24 Feb 2009 23:14:03 -0700
19, 30 -- Reply-To: {dkloos-at-parallaxray.com}
19, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
19, 30 -- To: {analytic-at-rawbw.com}
19, 30 -- Cc: {microscopy-at-microscopy.com}
19, 30 -- References: {200902250344.n1P3iEOR001481-at-ns.microscopy.com}
19, 30 -- Subject: RE: [Microscopy] viaWWW: broken window on EDX detector
19, 30 -- Date: Tue, 24 Feb 2009 22:13:57 -0800
19, 30 -- Organization: Parallax Research
19, 30 -- Message-ID: {10EBC7CC5AE4459C951BEDDA785E777F-at-donl}
19, 30 -- MIME-Version: 1.0
19, 30 -- Content-Type: text/plain;
19, 30 -- charset="us-ascii"
19, 30 -- Content-Transfer-Encoding: 7bit
19, 30 -- X-Mailer: Microsoft Office Outlook 11
19, 30 -- Thread-Index: AcmW+1227vOslqw6Se+IU2S7fmdh+gAE15+Q
19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 30 -- In-Reply-To: {200902250344.n1P3iEOR001481-at-ns.microscopy.com}
19, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
19, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
19, 30 -- X-AntiAbuse: Original Domain - microscopy.com
19, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
19, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: a.d.mckinnon-at-abdn.ac.uk
Date: Wed, 25 Feb 2009 03:41:40 -0600
Subject: [Microscopy] viaWWW: Epon/PAS stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Anne-Marie,

You would probably have to etch the resin to get PAS to work on epon sections (see recent lister communications re epoxy resin etching methods). You might also consider using methenamine silver as an alternative to get a more strongly defined end result, particularly if you are using semi-thin sections of 2 microns or less.
Alternatively if you were prepared to switch resin, PAS works on any acrylic resin but strongest with methyl methacrylate as you can easily remove the resin prior to staining.

Best regards,

Alastair

Alastair McKinnon
IMS Histology & EM Facility Manager, R2.21
University of Aberdeen, Institute of Medical Science
Foresterhill, Aberdeen, AB25 2ZD
tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab)
fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk
http://www.abdn.ac.uk/ims/histology/


-----Original Message-----
X-from: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu]
Sent: 25 February 2009 03:37
To: Mckinnon, Alastair D.

This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abrun-at-hsc.unt.edu
Name: Anne-Marie Brun

Organization: UNT HSC at Fort Worth Texas 76107, USA

Title-Subject: [Filtered] Epon/PAS stain

Question: Has anyone ever stained for PAS on Epon sections before? If not on Epon then what type of plastic did you use to do a PAS stain?
I know it is done on paraffin embedded material, but an investigator wants the PAS staining done on Epon sections. ----?
Thanks for your help
Anne-Marie

Login Host: 129.120.96.228
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:32 2009 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3RVv3007908
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:31 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5ca6b1378b7-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:29 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: Epon/PAS stain 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


The University of Aberdeen is a charity registered in Scotland, No SC013683.


==============================Original Headers==============================
21, 31 -- From a.d.mckinnon-at-abdn.ac.uk Wed Feb 25 03:41:38 2009
21, 31 -- Received: from mailhub4.abdn.ac.uk (mailhub4.abdn.ac.uk [139.133.7.214])
21, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P9fYXg002183
21, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 03:41:35 -0600
21, 31 -- Received: from ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83] helo=mail.abdn.ac.uk)
21, 31 -- by mailhub4.abdn.ac.uk with esmtp (Exim 4.69)
21, 31 -- (envelope-from {a.d.mckinnon-at-abdn.ac.uk} )
21, 31 -- id 1LcGGj-00085d-Ri; Wed, 25 Feb 2009 09:41:33 +0000
21, 31 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.82]) by
21, 31 -- ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83]) with mapi; Wed, 25 Feb 2009
21, 31 -- 09:41:33 +0000
21, 31 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk}
21, 31 -- To: "'abrun-at-hsc.unt.edu'" {abrun-at-hsc.unt.edu}
21, 31 -- CC: "'Microscopy-at-microscopy. com (Microscopy-at-microscopy.com)'"
21, 31 -- {Microscopy-at-microscopy.com}
21, 31 -- Date: Wed, 25 Feb 2009 09:41:32 +0000
21, 31 -- Subject: RE: [Microscopy] viaWWW: Epon/PAS stain
21, 31 -- Thread-Topic: [Microscopy] viaWWW: Epon/PAS stain
21, 31 -- Thread-Index: AcmW+mJPfRmEdFCbTLiTV5zWUAhCDQAMFtgw
21, 31 -- Message-ID: {A3317CA649BC8C4998EED327D66A8D95A8C4C87A42-at-VMAILB.uoa.abdn.ac.uk}
21, 31 -- References: {200902250337.n1P3bCK2021502-at-ns.microscopy.com}
21, 31 -- In-Reply-To: {200902250337.n1P3bCK2021502-at-ns.microscopy.com}
21, 31 -- Accept-Language: en-US
21, 31 -- Content-Language: en-US
21, 31 -- X-MS-Has-Attach:
21, 31 -- X-MS-TNEF-Correlator:
21, 31 -- acceptlanguage: en-US
21, 31 -- Content-Type: text/plain; charset="us-ascii"
21, 31 -- MIME-Version: 1.0
21, 31 -- Content-Transfer-Encoding: 8bit
21, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1P9fYXg002183
==============================End of - Headers==============================




From: lamiller-at-illinois.edu
Date: Wed, 25 Feb 2009 06:46:27 -0600
Subject: [Microscopy] OK, one last try, PAS: url to pdf

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://treefrog.cvm.uiuc.edu/methods/PAS_EPOXY_CMI_UIUC.pdf





{ { { { { { { { {} } } } } } } } } } } } } }
Lou Ann Miller, Service Supervisor
Center for Microscopic Imaging
College of Veterinary Medicine
University of Illinois MC=002

Room 1204 VMBSBld
2001 S Lincoln Ave
Urbana, IL 61821

217-244-1567
http://treefrog.cvm.uiuc.edu






==============================Original Headers==============================
13, 16 -- From lamiller-at-illinois.edu Wed Feb 25 06:46:26 2009
13, 16 -- Received: from expredir5.cites.uiuc.edu (expredir5.cites.uiuc.edu [128.174.5.96])
13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PCkPCQ023780
13, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 06:46:26 -0600
13, 16 -- Received: from beowulf.cvm.uiuc.edu (beowulf.cvm.uiuc.edu [130.126.16.163])
13, 16 -- by expredir5.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id n1PCkOPv027122
13, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 06:46:24 -0600 (CST)
13, 16 -- Message-Id: {8E54D6D8-1D2E-4071-BDA6-7853FBCDA1F7-at-illinois.edu}
13, 16 -- From: Lou Ann Miller {lamiller-at-illinois.edu}
13, 16 -- To: Microscopy-at-microscopy.com
13, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
13, 16 -- Content-Transfer-Encoding: 7bit
13, 16 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 16 -- Subject: OK, one last try, PAS: url to pdf
13, 16 -- Date: Wed, 25 Feb 2009 06:46:24 -0600
13, 16 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: smalinskas-at-yahoo.com
Date: Wed, 25 Feb 2009 07:35:28 -0600
Subject: [Microscopy] Re: Cleaning aperture strip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby,

I've always cleaned mine using diamond polishing compound. Since we are a metallographic laboratory, we have the polishing materials readily available. I use a 3-micron diamond solution with a cloth or felt pad. With the tip of my finger I swirl the aperture on the pad until the deposits are gone. This is somewhat risky, as an aperture corner can catch and fold onto itself. But this hasn't happened to me yet in the 15 years I've been doing this. I was introduced to this technique by Ken Converse of Quality Images.

Service techs are taught to hold one end of the aperture and use a Q-tip with diamond solution, rubbing in one direction only, away from where you're holding it. This polishes the aperture strip without the danger of folding.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan

--- On Tue, 2/24/09, dsherman-at-purdue.edu {dsherman-at-purdue.edu} wrote:
} Hi all,
}
} I have a number of very pricey aperture strips that need
} cleaned. The best
} way would be with a plasma cleaner. Does anyone have one
} who would be
} willing to try to clean a strip or can you recommend
} somewhere that I could
} send the strips for cleaning?
}
} Debby
}
} --
} Debby Sherman, Director Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www.agriculture.purdue.edu/microscopy/
}




==============================Original Headers==============================
8, 22 -- From smalinskas-at-yahoo.com Wed Feb 25 07:35:28 2009
8, 22 -- Received: from web34401.mail.mud.yahoo.com (web34401.mail.mud.yahoo.com [66.163.178.150])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1PDZQq2006674
8, 22 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 25 Feb 2009 07:35:27 -0600
8, 22 -- Received: (qmail 96695 invoked by uid 60001); 25 Feb 2009 13:35:25 -0000
8, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1235568924; bh=JDGGC3tPXclQ5i3508oRXSEfOL7Ay9VcFslokmFDruo=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type; b=v+vtv9kjIi/R4wBFW2pcQkbTgeqkqx16/gNohZ1i8x/WONXx/SNrCtyTHbv9s7nGIb0kdHPxAeHYxP5yPstHPU7vdi//I+AdL547+jGP/xnkUMHLjtnz6oMX3OFIGIaAll2/0eS65jqiHm7rOkON8LHR3EhV7ZFyLxKZN5ihHBw=
8, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 22 -- s=s1024; d=yahoo.com;
8, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type;
8, 22 -- b=3Gt/8YYXjjFNF0Of5MITzSCI9zHUtZipnSxp8s7JHvqno9iCGsMLD36Mk3cbi49fn4A7OmR1hl4pxxzxNRTtsmgbVALVQQKbWxaOwJysTXYNIdWC9vj7yMkti6MEBqDmDf1mAQl0GoJX7qcMHSse6/c4TMF6ysfdLNB708/rfNk=;
8, 22 -- Message-ID: {962274.93598.qm-at-web34401.mail.mud.yahoo.com}
8, 22 -- X-YMail-OSG: frSV7.kVM1kFvr0NEVYddebvYATysbcmuaULPCFa_fThOjy3Uxpcux2HNROyzY9vltdQ5CmHfTGYi4lTI1RluPNPNAARl2Z5JHog5xo7BGW6imQg14Osn2go5g1pjYBwxcM8xnfcNZ7rm7O7_HzJwqre2SqMqi2LIzI4IJ3_3WV3nWUcOtLRfmqVC6asV7h8rK01jZPuppLqR.qLzOEX0sPVgW5.QUE-
8, 22 -- Received: from [141.151.33.213] by web34401.mail.mud.yahoo.com via HTTP; Wed, 25 Feb 2009 05:35:24 PST
8, 22 -- X-Mailer: YahooMailWebService/0.7.289.1
8, 22 -- Date: Wed, 25 Feb 2009 05:35:24 -0800 (PST)
8, 22 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
8, 22 -- Reply-To: smalinskas-at-yahoo.com
8, 22 -- Subject: Re: [Microscopy] Cleaning aperture strip
8, 22 -- To: dsherman-at-purdue.edu, microscopy-at-ns.microscopy.com
8, 22 -- In-Reply-To: {200902242205.n1OM5J1H027772-at-ns.microscopy.com}
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: ahmadsam-at-sabic.com
Date: Wed, 25 Feb 2009 08:11:15 -0600
Subject: [Microscopy] viaWWW: Power Supply Board for Computer with XL-30 Embaded with

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmadsam-at-sabic.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmadsam-at-sabic.com
Name: Dr Shahreer Ahmad

Organization: SABIC Technology Center

Title-Subject: [Filtered] Power Supply Board for Computer with XL-30
Embaded with EDAX 4i system

Question: Can anyone help me to get a power supply board Part Number
5335 171 03030 : PC Power supply, PS2, 350W. specially, if a system
is not in use with someone. Manufacturer declined to supply as the
system in obsolete.

Best regards.

Dr. Shahreer Ahmad,
SABIC Technology Center,
P.O.Box 11669,
Al-Jubail 31961,
Saudi Arabia
Phone: +966-3-359-9175
Cell: +966-508491532
Email: ahmadsam-at-sabic.com


Login Host: 89.147.0.109
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Wed Feb 25 08:11:14 2009
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PEBD1b021784
9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:11:13 -0600
9, 12 -- Mime-Version: 1.0
9, 12 -- Message-Id: {p06240802c5cb01edd485-at-[206.69.208.22]}
9, 12 -- Date: Wed, 25 Feb 2009 08:11:12 -0600
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: ahmadsam-at-sabic.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: Power Supply Board for Computer with XL-30 Embaded with
9, 12 -- EDAX 4i system
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nama1213-at-hotmail.com
Date: Wed, 25 Feb 2009 08:11:50 -0600
Subject: [Microscopy] viaWWW: Cryosectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nama1213-at-hotmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nama1213-at-hotmail.com
Name: Dr. Naemah Al-Mansour

Organization: Kuwait University

Title-Subject: [Filtered] Cryosectioning

Question: Dear respected EMU users
Does anyone know a standard technique to prepare frozen sections from
fresh plant, liver and kidney samples? Usually I fix tissue in a
suitable fixative depending on tissue type and objective of study
(e.g. diagnostic, routine light microscopy work, TEM ...etc.) but
would like to know best way for preparation of fresh frozen sections
using cryostat instruments. I would be grateful if anyone can help
or give suggestions on that matter stating any manual to follow for
successful work in biological sample preparation. I really appreciate
your help

Best regards
Dr. Naemah Al-Mansour
Stress Ecophysiologist
Kuwait University - Faculty of Science
Department of Biological Sciences
Office 59
POBOX 5969 Safat 13060
State of Kuwait

Office: 24985603




Login Host: 78.89.1.123
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Feb 25 08:11:48 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PEBjIK022435
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:11:46 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c5cb020bdb7b-at-[206.69.208.22]}
11, 11 -- Date: Wed, 25 Feb 2009 08:11:44 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: nama1213-at-hotmail.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Cryosectioning
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lamiller-at-illinois.edu
Date: Wed, 25 Feb 2009 08:12:18 -0600
Subject: [Microscopy] viaWWW: Epoxy Section PAS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lamiller-at-illinois.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lamiller-at-illinois.edu
Name: Lou Ann Miller

Organization: University of Illinois

Title-Subject: [Filtered] Epoxy Section PAS

Question:
PAS on EPOXY Sections


Sometimes it is desired to verify components in an epoxy section for =20
PAS positive. Because the TEM epoxy blocks have ran the tissue through =20=

many different processes than the histology tissues go through, it is =20=

necessary to run the procedure differently, and understand the =20
coloration of the final product may be different.

The first problem is the hardness of the epoxy, stains, especially at =20=

room temperature do not always stain epoxified sections well. The =20
second is that the fixative used in regular Transmission Electron =20
Microscopy contains gluteraldehyde which will cause false positive =20
staining if not delt with. The use of saturated Potassium Hydroxide =20
in Methanaol, used first off on the section to etch it, seems to take =20=

care of both of these issues.

The tissue has also gone many reactions and exposures to heavy metals =20=

as well, osmium, and uranyl acetate in the processing. These chemicals =20=

change the tissue in a way that histology tissues are not, pretty =20
bright magenta pinks are not seen. Instead a darker gray area is seen, =20=

in very thick places or sections, some of the pink may show through =20
somewhat.

It is because of this coloration, that for some instances, a counter =20
stain is not used, especially the normal 0.5% Tolluidine Blue with 1% =20=

Borate, as it will cover and obliterate the staining.


Materials needed:
Hotplate
beakers
dispopipets
kimwipes
staining pad


Chemicals Used
Saturated Potassium Hydroxide in Methanol
1 part KOH pellets, 2 parts MeOH, let set 1-3 days before using, will =20=

have a remaining slurry of KOH on the bottom

0.5 % Periodic Acid

Shift Reagent - Borrowed from Regular Histology Lab for their PAS

Water

Procedure

1. Cut epoxy sections, for this procedure, 0.33 to 0.5 microns =20
thick, and dry down well on a hotplate. Our thermolyne hotplate is set =20=

at 120. Dry at least one hour on the hotplate.

2. Remove slides and set the hotplate dow to 60 to cool.

3. At room temperature, with the slide level, apply the saturated KOH-=20=

MeOH mixture and allow to incubate for 20-40 minutes.

4. Rinse off gently with water, and allow to sit in water rinse baths =20=

for at least 10-15 minutes.


5. Air dry off the slide gently ( hose to air outlet with filter on =20
end) and place on the 60 set hotplate.

6. Immediately flood the slide with 0.5% Periodic Acid. Let set for =20=

40 minutes, periodically checking to be sure the slide does not =20
evaporate the solution away. Add more solution as needed.

7. Rinse off gently with water, and allow to sit in water rinse baths =20=

for at least 10-15 minutes.

8. Air dry off the slide gently ( hose to air outlet with filter on =20
end) and place on the 60 set hotplate.


9. Immediately flood the slide with the standard Shift=92s reagent from =20=

Histology. Allow to stay on the hotplate for 40-60 minutes, again =20
checking that the solution does not evaporate and adding more solution =20=

as necessary.

10. Rinse with water well, and soak in water baths for up to 15 minutes.

11. Dry slide and observe.





References:

Hayat, M.A., =93Stains and Cytochemical Methods=94, Plenum Press, New =20=

York, 1993, p 64





|--|--|--|--|--|--|--|--|----|--|--|--|--|--|--|--|--|--|--|--|--|--|
Lou Ann Miller, Service Supervisor
Center for Microscopic Imaging
College of Veterinary Medicine
University of Illinois MC=3D002

Room 1204 VMBSBld
2001 S Lincoln Ave
Urbana, IL 61821

217-244-1567
http://treefrog.cvm.uiuc.edu


Login Host: 130.126.16.163
---------------------------------------------------------------------------

==============================Original Headers==============================
63, 11 -- From zaluzec-at-microscopy.com Wed Feb 25 08:12:17 2009
63, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
63, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PECF8r023811
63, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:12:17 -0600
63, 11 -- Mime-Version: 1.0
63, 11 -- Message-Id: {p06240804c5cb022fe3df-at-[206.69.208.22]}
63, 11 -- Date: Wed, 25 Feb 2009 08:12:15 -0600
63, 11 -- To: microscopy-at-microscopy.com
63, 11 -- From: lamiller-at-illinois.edu (by way of MicroscopyListserver)
63, 11 -- Subject: viaWWW: Epoxy Section PAS
63, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dfish6101-at-gmail.com
Date: Wed, 25 Feb 2009 08:13:54 -0600
Subject: [Microscopy] viaWWW: SEM - XL30 computer problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dfish6101-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dfish6101-at-gmail.com
Name: David Fisher

Organization: Superior Technical Services

Title-Subject: [Filtered] SEM - XL30 computer problems

Question: Steve,
Tell more about your computer and the video card you are talking about.

-OS?
-VGA card (typically ATI Mach 64) PCI or ISA?
-Overlay card (typically Coreco MX)?
-Which one are you reseating?

Is the "red screen" you mention a Windows memory dump screen similar
to the Blue Screen Of Death?

We have identified similar problems being caused by the power supply.
Either very noisy AC going into the computer, or a weak PC supply,
specifically the 3.3V.

More details will be helpful, as we may have surplus spares.
Although the video boards are rare and valuable you should be able to
solve your problem for significantly less than $15k AU.

Dave
David Fisher
Superior Technical Services
16307 NE Cameron Blvd.
Portland, OR 97230
(503) 764-1456 x3

Login Host: 24.97.255.26
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Feb 25 08:13:54 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PEDq1T029488
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:13:53 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240805c5cb0285f824-at-[206.69.208.22]}
11, 11 -- Date: Wed, 25 Feb 2009 08:13:50 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dfish6101-at-gmail.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: SEM - XL30 computer problems
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Wed, 25 Feb 2009 08:45:58 -0600
Subject: [Microscopy] Re: viaWWW: Cryosectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Al-Mansour-
For a number of years, we have used a variation on the protocol outlined in:
Barthel & Raymond (1990) J. Histochem. Cytochem 38(9) 1283-1388
the authors were working with eyes, which are expecially difficult due.

Briefly, after fixation with pfa (2% or 4% depending on your
requirements) we cryoprotect in a 30% sucrose in PBS, then
encapsulate and freeze in a 1:2 mixture of the 30% sucrose and OCT.
This yields smooth-cutting blocks and remarkable good structure.
My facility is in a medical college, so we deal exclusively with
(mammalian) tissue samples. I can't tell you anything about working
with plant material.

Lee




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
10, 25 -- From lcgould-at-med.cornell.edu Wed Feb 25 08:45:57 2009
10, 25 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PEjv0M012678
10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:45:57 -0600
10, 25 -- Received: from mpx2.med.cornell.edu (pc113142-13.med.cornell.edu [140.251.11.119])
10, 25 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n1PEjHoG003402;
10, 25 -- Wed, 25 Feb 2009 09:45:55 -0500 (EST)
10, 25 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
10, 25 -- by mpx2.med.cornell.edu
10, 25 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
10, 25 -- with ESMTPA id {0KFM00I7PMCENX50-at-mpx2.med.cornell.edu} ; Wed,
10, 25 -- 25 Feb 2009 09:45:51 -0500 (EST)
10, 25 -- Date: Wed, 25 Feb 2009 09:45:45 -0500
10, 25 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
10, 25 -- Subject: Re: [Microscopy] viaWWW: Cryosectioning
10, 25 -- In-reply-to: {200902251426.n1PEQZGR006428-at-ns.microscopy.com}
10, 25 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
10, 25 -- To: Microscopy Listserver {microscopy-at-microscopy.com} , nama1213-at-hotmail.com
10, 25 -- Message-id: {p06230905c5cb08c7ed30-at-[140.251.48.23]}
10, 25 -- MIME-version: 1.0
10, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed
10, 25 -- Content-transfer-encoding: 7BIT
10, 25 -- References: {200902251426.n1PEQZGR006428-at-ns.microscopy.com}
10, 25 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.25.143428
10, 25 -- X-PerlMx-Spam: Gauge=, IIIIIII, Probability=7%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_GREETING 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0'
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 25 Feb 2009 08:47:54 -0600
Subject: [Microscopy] viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Margo,
I believe that the "common knowledge" is not always correct. My
understanding is that the SiLi crystal is usually destroyed by having the
bias applied when warm. One of the ways to avoid that is to always turn the
bias off before unplugging the detector. The bias supply will drain the
voltage off, whereas unplugging the bias will leave a small, but very
efficient, capacitor charged to the bias voltage for, perhaps, longer than
it takes the detector to warm up.

The broken window may actually be your only problem, although still
expensive to repair, in part because the window is expensive and the dewar
must be repumped and leak-checked.

My customers have had good experiences with Jim Nicholino. No financial
interest, but I like my customers to be happy.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: analytic-at-rawbw.com [mailto:analytic-at-rawbw.com]
Sent: Tuesday, February 24, 2009 10:33 PM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both analytic-at-rawbw.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: analytic-at-rawbw.com
Name: Margo Gill-Linscott

Organization: Analyticus, Inc.

Title-Subject: [Filtered] broken window on EDX detector

Question: I understand that once a 'window' on an EDX detector is
broken there is no way to repair it and the crystal is destroyed. Is
this true and does this apply to all types of detectors?

Login Host: 198.144.223.152
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:58 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1P3Rt9m008169
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:58
-0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c5ca6b297dc1-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:55 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: broken window on EDX detector
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
20, 25 -- From kenconverse-at-qualityimages.biz Wed Feb 25 08:47:54 2009
20, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PElsM1016611
20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 08:47:54 -0600
20, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
20, 25 -- with ESMTP
20, 25 -- id {20090225144753.QKWD20000.cdptpa-omta03.mail.rr.com-at-Ken} ;
20, 25 -- Wed, 25 Feb 2009 14:47:53 +0000
20, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
20, 25 -- To: {analytic-at-rawbw.com} , "MSA Listserver" {microscopy-at-microscopy.com}
20, 25 -- Subject: RE: [Microscopy] viaWWW: broken window on EDX detector
20, 25 -- Date: Wed, 25 Feb 2009 09:47:47 -0500
20, 25 -- Message-ID: {BE1038D693CE4DF7B541C2EF78019490-at-Ken}
20, 25 -- MIME-Version: 1.0
20, 25 -- Content-Type: text/plain;
20, 25 -- charset="US-ASCII"
20, 25 -- X-Priority: 3 (Normal)
20, 25 -- X-MSMail-Priority: Normal
20, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
20, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 25 -- Thread-Index: AcmW+dRSt42OJVhhSVq/XVetIxEOjQAXOuAQ
20, 25 -- Importance: Normal
20, 25 -- In-Reply-To: {200902250333.n1P3XTvp015874-at-ns.microscopy.com}
20, 25 -- Content-Transfer-Encoding: 8bit
20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1PElsM1016611
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Wed, 25 Feb 2009 09:07:05 -0600
Subject: [Microscopy] Re: viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Margo,

If a window on a detector blows, it depends upon how it blew will
determine if the crystal is damaged or not.

If the detector never sees atmosphere and is undamaged, it can run with
the broken window without a problem.

Another source for fixing EDS detectors is Garry Baerwalt of Max Detectors
in Middletown Wisconsin. I don't remember the address but his email
address is garry-at-maxdetector.com

Good luck,

dj


On Tue, 24 Feb 2009, analytic-at-rawbw.com wrote:

} Email: analytic-at-rawbw.com
} Name: Margo Gill-Linscott
}
} Organization: Analyticus, Inc.
}
} Title-Subject: [Filtered] broken window on EDX detector
}
} Question: I understand that once a 'window' on an EDX detector is
} broken there is no way to repair it and the crystal is destroyed. Is
} this true and does this apply to all types of detectors?
}
} Login Host: 198.144.223.152
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:58 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3Rt9m008169
} 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:58 -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240801c5ca6b297dc1-at-[206.69.208.22]}
} 6, 11 -- Date: Tue, 24 Feb 2009 21:27:55 -0600
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: broken window on EDX detector
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
9, 19 -- From dljones-at-bestweb.net Wed Feb 25 09:07:05 2009
9, 19 -- Received: from smtp1.bestweb.net (smtp1.bestweb.net [209.94.103.41])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PF75Jh008510
9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 09:07:05 -0600
9, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202])
9, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 19 -- (No client certificate requested)
9, 19 -- by smtp1.bestweb.net (Postfix) with ESMTPS id 72735125434;
9, 19 -- Wed, 25 Feb 2009 10:07:04 -0500 (EST)
9, 19 -- Date: Wed, 25 Feb 2009 14:39:34 +0000 (UTC)
9, 19 -- From: dljones {dljones-at-bestweb.net}
9, 19 -- To: analytic-at-rawbw.com
9, 19 -- cc: microscopy-at-microscopy.com
9, 19 -- Subject: Re: [Microscopy] viaWWW: broken window on EDX detector
9, 19 -- In-Reply-To: {200902250335.n1P3Z9ol018420-at-ns.microscopy.com}
9, 19 -- Message-ID: {Pine.BSF.4.64.0902251422380.94009-at-monet.bestweb.net}
9, 19 -- References: {200902250335.n1P3Z9ol018420-at-ns.microscopy.com}
9, 19 -- MIME-Version: 1.0
9, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: vlynch-at-mail.wsu.edu
Date: Wed, 25 Feb 2009 10:29:47 -0600
Subject: [Microscopy] Philips TEM CM-200 Manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jesse,
Thank you for the clarification. And please relay my thanks to Sheri
Kurland, Mark Huller and Steve Gazda for their excellent service and
the amazing speed at which they found and sent us a manual for our
CM200.
Sincerly,
Valerie

} Val,
}
} Just to clarify, it was EDAX that destroyed the last of their manuals
} for your detector. We still have copies of ours. I'm working on
} finding them for you though. Most are in use out at customers' sites or
} for internal reference.
}
} Jesse Doerr
}
} Field Service Engineer
} FEI Company
} (503)267-9620
} jesse.doerr-at-fei.com
}
} -----Original Message-----
} From: vlynch-at-mail.wsu.edu [mailto:vlynch-at-mail.wsu.edu]
} Sent: Thursday, February 05, 2009 1:25 PM
} To: Doerr, Jesse
} Subject: [Microscopy] RE: Philips TEM CM-200 Manual?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America


==============================Original Headers==============================
3, 24 -- From vlynch-at-mail.wsu.edu Wed Feb 25 10:29:46 2009
3, 24 -- Received: from mx.wsu.edu.pph4a.pphosted.com (mx.wsu.edu.pph4a.pphosted.com [208.84.65.34])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PGThAt025934
3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 10:29:45 -0600
3, 24 -- Received: from bobcat.it.wsu.edu (bobcat.it.wsu.edu [134.121.0.132])
3, 24 -- by mx.wsu.edu.pph4a.pphosted.com (8.14.1/8.14.1) with ESMTP id n1PGTbD0003706;
3, 24 -- Wed, 25 Feb 2009 08:29:37 -0800
3, 24 -- Received: from [134.121.42.104] (vrf.emc.wsu.edu [134.121.42.104])
3, 24 -- by bobcat.it.wsu.edu (8.12.11.20060308/8.12.11) with ESMTP id n1PGTafL030740;
3, 24 -- Wed, 25 Feb 2009 08:29:37 -0800
3, 24 -- Mime-Version: 1.0
3, 24 -- Message-Id: {a06200701c5cb204762b0-at-[134.121.42.104]}
3, 24 -- In-Reply-To: {A6E613CC1F9FD34B9A5EEE7DC4590AE3A0DE44-at-hlexc04.w2k.feico.com}
3, 24 -- References: {200902052125.n15LP10P018229-at-ns.microscopy.com}
3, 24 -- {A6E613CC1F9FD34B9A5EEE7DC4590AE3A0DE44-at-hlexc04.w2k.feico.com}
3, 24 -- Date: Wed, 25 Feb 2009 08:29:35 -0800
3, 24 -- To: "Doerr, Jesse" {Jesse.Doerr-at-fei.com}
3, 24 -- From: Valerie Lynch-Holm {vlynch-at-mail.wsu.edu}
3, 24 -- Subject: RE: [Microscopy] RE: Philips TEM CM-200 Manual?
3, 24 -- Cc: microscopy-at-microscopy.com
3, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 24 -- X-Virus-Scanned: by amavisd-milter (http://amavis.org/)
3, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-25_14:2009-02-10,2009-02-25,2009-02-25 signatures=0
3, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0902250103
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 25 Feb 2009 11:25:54 -0600
Subject: [Microscopy] viaWWW: broken window on EDX detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I may have to stand correctable here but
my experience says that the bias will
automatically shut off if the FET and detector
crystal are not cold enough. When the window
breaks, that ought to cause the -750V to shut
off.

The detector is a reverse biased drifted Silicon
diode and the reverse bias creates a big depletion
region. The output from the detector crystal is
fed to a FET amplifier transistor which is also
cooled to reduce noise. Its signal is then sent
up stream to room temperature electronics and on
to the pulse processor in the PC.

Since the detector probe is under vacuum, venting
the SEM chamber too fast or pulling open the door
before full venting will likely crack the EDS
window. AFIK, all makers use Moxtec windows, mostly
the .3u thick SUTW. The detectors with no windows
are a separate subject.

gary g.


At 06:49 AM 2/25/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Wed Feb 25 11:25:54 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1PHPscQ010023
10, 20 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 11:25:54 -0600
10, 20 -- Message-Id: {200902251725.n1PHPscQ010023-at-ns.microscopy.com}
10, 20 -- Received: (qmail 5507 invoked from network); 25 Feb 2009 09:24:16 -0800
10, 20 -- Received: by simscan 1.1.0 ppid: 5500, pid: 5503, t: 0.1905s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 25 Feb 2009 09:24:15 -0800
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Wed, 25 Feb 2009 09:25:48 -0800
10, 20 -- To: kenconverse-at-qualityimages.biz
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] RE: viaWWW: broken window on EDX detector
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200902251449.n1PEnHU4021273-at-ns.microscopy.com}
10, 20 -- References: {200902251449.n1PEnHU4021273-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: nicholas.ritchie-at-nist.gov
Date: Wed, 25 Feb 2009 12:44:08 -0600
Subject: [Microscopy] Microanalysis of Particles 2009 - Registration closes 1-Mar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suppose the new detectors might have a circuit to shut off HV,
but I can certainly say that many old detectors did not. We have such a
one still in service. We lost one crystal because it warmed up under
improper conditions.

I suppose it would be the pressure pulse that would rupture the window.
We have not had one fail due to anyone slamming the SEM chamber closed
or pulling it open - yet. I don't care to run the experiment on my
nickel. Maybe the guys at Moxtek have run those experiments as part of
their R&D.

Warren Straszheim

-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Wednesday, February 25, 2009 11:27 AM
To: wesaia-at-iastate.edu

/*****************************************************************
* Microbeam Analysis Society *
* presents *
* Microanalysis of Particles 2009 *
* Topical Conference *
* *
* 20-23 April 2009 *
* Westmont, IL *
*****************************************************************/

Download the full program:
http://www.microbeamanalysis.org/meetings/topical/Particles2009/Program.pdf

Register for the meeting: (by 1-Mar-2009)
http://www.microbeamanalysis.org/meetings/topical/Particles2009/registration.htm

Synopsis:

Particles represent a microanalytical challenge. Whether
your particles are desired or undesired, natural or man-made,
nano-, micro- or milli-scaled, no one technique is optimal
for determining all the important physical properties.
A knowledgable analyst must understand the strengths and
weaknesses of each available technique. This conference is
designed to provide just such an understanding for microbeam
techniques including EDS, WDS, AEM, SIMS, XRD, synchrotron
XRF and numerical simulation. The target audience includes
scientists in industry, government, academia and commercial
laboratories. The speakers will provide enough background for
a motivated novice but will aim the majority of the content
at practitioners who wish to learn new techniques and to pick
up advanced skills.



==============================Original Headers==============================
7, 22 -- From nicholas.ritchie-at-nist.gov Wed Feb 25 12:44:08 2009
7, 22 -- Received: from smtp.nist.gov (rimp2.nist.gov [129.6.16.227])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PIi8NJ008795
7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 12:44:08 -0600
7, 22 -- Received: from [129.6.126.23] (smsd-fw.nist.gov [129.6.126.23])
7, 22 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id n1PIi4rP010317
7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:44:04 -0500
7, 22 -- Received: from no.name.available by [129.6.126.23]
7, 22 -- via smtpd (for [129.6.16.228] [129.6.16.228]) with ESMTP; Wed, 25 Feb 2009 13:52:38 -0500
7, 22 -- Message-ID: {49A59174.9070100-at-nist.gov}
7, 22 -- Date: Wed, 25 Feb 2009 13:44:04 -0500
7, 22 -- From: Nicholas Ritchie {nicholas.ritchie-at-nist.gov}
7, 22 -- Reply-To: nicholas.ritchie-at-nist.gov
7, 22 -- Organization: NIST
7, 22 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: Microscopy-at-microscopy.com
7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-NIST-MailScanner: Found to be clean
7, 22 -- X-NIST-MailScanner-From: nicholas.ritchie-at-nist.gov
7, 22 -- Subject: Microanalysis of Particles 2009 - Registration closes 1-Mar
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Wed, 25 Feb 2009 13:19:55 -0600
Subject: [Microscopy] Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This thread brought up a question.

Suppose someone wants to mothball a LN2 chilled detector for a period of time.

Is this feasible without significant damage?

What precautions should be taken (bias off and voltage drained) prior
to warming up, etc?

What are the negative aspects of doing this (loss of resolution, presumably)?

Thanks for the information.
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Wed Feb 25 13:19:55 2009
8, 19 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PJJtov024308
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:55 -0600
8, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
8, 19 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n1PJJrDS001332
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:54 -0600 (CST)
8, 19 -- Mime-Version: 1.0
8, 19 -- Message-Id: {a06240802c5cb492177ae-at-[131.230.177.136]}
8, 19 -- In-Reply-To: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- References: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- Date: Wed, 25 Feb 2009 13:19:52 -0600
8, 19 -- To: Microscopy-at-microscopy.com
8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 19 -- Subject: [Microscopy] Another EDX detector question
8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 19 -- X-Spam-Score: 0.00%
8, 19 -- X-MASF: 0.00%
8, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Wed, 25 Feb 2009 13:36:42 -0600
Subject: [Microscopy] Re: Laser Surface Profiler

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roy Beavers asked about measuring the height profile of a polyimide
laser-treated surface with nanometer resolution. He wrote: "SEM images have
been very difficult to achieve due to charging and small feature size.
Thinking a surface profiler or AFM may give better results."

We have been providing commercial AFM analysis services for 19 years. We
have unique capabilities, which include the ability to measure small heights
with fine resolution (to 0.2 nm, i.e. single atomic steps) and to measure
extremely tall objects (to 24 um) with resolution about 1 nm.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================
----- Original Message -----
From: rbeavers-at-mail.smu.edu
To: donc-at-asmicro.com
Sent: Wednesday, February 18, 2009 1:54 PM
Subject: [a] [Microscopy] Laser Surface Profiler





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Group,

Trying to locate a lab in the North Texas area that has Laser 3D Surface
profile capabilities. Need to profile surface of a laser treated polyimide
film on glass substrate to nanometer resolution. SEM images have been very
difficult to achieve due to charging and small feature size. Thinking a
surface profiler or AFM may give better results.

Prefer University labs but will be glad to hear from commercial labs as
well.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu


regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
16, 24 -- From donc-at-asmicro.com Wed Feb 25 13:36:41 2009
16, 24 -- Received: from smtp121.sbc.mail.re3.yahoo.com (smtp121.sbc.mail.re3.yahoo.com [66.196.96.94])
16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1PJaffn006063
16, 24 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:36:41 -0600
16, 24 -- Received: (qmail 80788 invoked from network); 25 Feb 2009 19:36:41 -0000
16, 24 -- Received: from unknown (HELO asm15) (donc-at-76.240.194.228 with login)
16, 24 -- by smtp121.sbc.mail.re3.yahoo.com with SMTP; 25 Feb 2009 19:36:40 -0000
16, 24 -- X-YMail-OSG: cY6U49AVM1lNLUGonUcIjLVD9SuidUvA0rMG9P8OdFMBj6aNeQjbjTvWnqRYC38Ays5d.PihTnCRLNyYQQ3w.zAwqtJSBOJPUPM4DuW_mFWSjjFyVP_6wz.9FH06Nepivw6UwbcE7qtUfc1YLduyLlt_zfLG8KAl68qpBIKZXLTOI3eqWzTLtkLz_w1un9FyKl2VkQNK6fOUvA7ReaKHgjoZ.JXlyci2eZM-
16, 24 -- X-Yahoo-Newman-Property: ymail-3
16, 24 -- Message-ID: {2B54D8F9A61647F49E28B33430BDEA42-at-asm15}
16, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
16, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
16, 24 -- Subject: Re: [Microscopy] Laser Surface Profiler
16, 24 -- Date: Wed, 25 Feb 2009 14:36:20 -0500
16, 24 -- MIME-Version: 1.0
16, 24 -- Content-Type: text/plain;
16, 24 -- format=flowed;
16, 24 -- charset="iso-8859-1";
16, 24 -- reply-type=original
16, 24 -- Content-Transfer-Encoding: 7bit
16, 24 -- X-Priority: 3
16, 24 -- X-MSMail-Priority: Normal
16, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
16, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 25 Feb 2009 13:49:27 -0600
Subject: [Microscopy] Re: Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Suppose someone wants to mothball a LN2 chilled detector for a period of time.
} Is this feasible without significant damage?
} What precautions should be taken (bias off and voltage drained) prior
} to warming up, etc?
} ?What are the negative aspects of doing this (loss of resolution, presumably)?

Hi John

I did this over a 1-month vacation period once, having been assured by the manufacturer that
it was perfectly OK.

The vacuum and resolution both deteriorated to the point of being unusable for quantitative
work.

After this had happened, and on further rather annoyed questioning, the manufacturer said
that some deterioration in performance was to be expected.

I chose to replace it, with one from a different maker!

I will never, never do this again unless it is 100% unavoidable.

cheers

Ritchie




--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
15, 30 -- From r.sims-at-auckland.ac.nz Wed Feb 25 13:49:26 2009
15, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34])
15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PJnP3r020068
15, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Feb 2009 13:49:26 -0600
15, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id A4D551A2C9;
15, 30 -- Thu, 26 Feb 2009 08:49:24 +1300 (NZDT)
15, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
15, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
15, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
15, 30 -- with ESMTP id R01SrwJsNjjA; Thu, 26 Feb 2009 08:49:24 +1300 (NZDT)
15, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
15, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
15, 30 -- (No client certificate requested)
15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 7ED311A284;
15, 30 -- Thu, 26 Feb 2009 08:49:23 +1300 (NZDT)
15, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
15, 30 -- To: bozzola-at-siu.edu
15, 30 -- Date: Thu, 26 Feb 2009 08:51:29 +1300
15, 30 -- MIME-Version: 1.0
15, 30 -- Subject: Re: [Microscopy] Another EDX detector question
15, 30 -- CC: Microscopy-at-Microscopy.Com
15, 30 -- Message-ID: {49A65811.27551.45F397-at-r.sims.auckland.ac.nz}
15, 30 -- Priority: normal
15, 30 -- In-reply-to: {200902251920.n1PJKcS4025632-at-ns.microscopy.com}
15, 30 -- References: {200902251920.n1PJKcS4025632-at-ns.microscopy.com}
15, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
15, 30 -- Content-type: text/plain; charset=US-ASCII
15, 30 -- Content-transfer-encoding: 7BIT
15, 30 -- Content-description: Mail message body
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 25 Feb 2009 13:59:15 -0600
Subject: [Microscopy] Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,
Hopefully others with more expertise in this area will chime in because my
information is old.

My understanding is that when Kevex brought out their windowless detector
(too many years ago), what they found was that warming, in and of itself,
was not a problem as long as the bias was gone. The big problem was
condensable contaminants that would be captured by the zeolites in the
dewar, released upon warming and then contaminating the SiLi crystal. They
were perfectly willing to ship those detectors warm, but the appendage pump
had a battery operated controller that shipped with it. Basically they were
far more concerned about the quality if the vacuum than the temperature of
the crystal.

We've all heard lots of stories about how one person's detector warmed up
once and was trashed, while others repeatedly warm their detectors and keep
right on truckin'. What I take from Kevex's experience is that it's kind of
a crap shoot and depends upon what is in the vacuum part of the dewar,
whether from manufacturing residues or from leaking and what condensables
might have entered through the leak(s).

The other slight risk with warming is that if you've had a bad leak for a
while, when the zeolites warm up, all the gasses they've captured are going
to be released, possibly going to considerable positive pressure and blowing
the window. It's probably not a concern unless your lN2 consumption is very
high, indicating a poor vacuum in the dewar, but thin windows make it more
of a concern than Be windows.

Is it PGT that made the LEAP detector? The dewar looks nothing like your
standard dewar, doesn't hold much lN2, and functions for years going warm
and cold, warm and cold. It may be that they got rid of the zeolites (hence
the sorption pumping) to eliminate the possibility of contaminating the
crystal upon warming. Maybe someone who has some info can comment on that.

I think the answer to your question is, "It's a crap-shoot." May the force
be with you.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Wednesday, February 25, 2009 2:22 PM
To: kenconverse-at-qualityimages.biz

This thread brought up a question.

Suppose someone wants to mothball a LN2 chilled detector for a period of
time.

Is this feasible without significant damage?

What precautions should be taken (bias off and voltage drained) prior
to warming up, etc?

What are the negative aspects of doing this (loss of resolution,
presumably)?

Thanks for the information.
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Wed Feb 25 13:19:55 2009
8, 19 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1PJJtov024308
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:55
-0600
8, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu
[131.230.177.136])
8, 19 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id
n1PJJrDS001332
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:54
-0600 (CST)
8, 19 -- Mime-Version: 1.0
8, 19 -- Message-Id: {a06240802c5cb492177ae-at-[131.230.177.136]}
8, 19 -- In-Reply-To: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- References: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- Date: Wed, 25 Feb 2009 13:19:52 -0600
8, 19 -- To: Microscopy-at-microscopy.com
8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 19 -- Subject: [Microscopy] Another EDX detector question
8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 19 -- X-Spam-Score: 0.00%
8, 19 -- X-MASF: 0.00%
8, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




==============================Original Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Wed Feb 25 13:59:15 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PJxEBq031361
25, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:59:14 -0600
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20090225195913.ZHOE20000.cdptpa-omta03.mail.rr.com-at-Ken} ;
25, 25 -- Wed, 25 Feb 2009 19:59:13 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: {bozzola-at-siu.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] Another EDX detector question
25, 25 -- Date: Wed, 25 Feb 2009 14:59:08 -0500
25, 25 -- Message-ID: {8769BB04EB5B45DCB461C784BBA81958-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="US-ASCII"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- Thread-Index: AcmXflHAu9xcsgraQs+YTApKmLuF6AAAX2TQ
25, 25 -- Importance: Normal
25, 25 -- In-Reply-To: {200902251921.n1PJLuQe028356-at-ns.microscopy.com}
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1PJxEBq031361
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 25 Feb 2009 15:34:02 -0600
Subject: [Microscopy] Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, that was what I concluded, the vacuum is everything, and in a windowed detector,
warming up can allow gases to be desorbed from the zeolites (or whatever "getter" is used).

If the vacuum deteriorates, the internals run a bit warmer because the thermal insulation is
compromised.

JEOL had for a while an integrated EDS detector which could be warmed and recooled
repeatedly because it used the SEM vacuum to revacuate the detector, as I understood it.
Seemed like a pretty good idea to me but I don't know if it was good in practice or if they still
offer it. Is that what that LEAP detector was?

cheers
Ritchie



On 25 Feb 2009 at 14:00, kenconverse-at-qualityimages.biz wrote:




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi John,
Hopefully others with more expertise in this area will chime in because my
information is old.

My understanding is that when Kevex brought out their windowless detector
(too many years ago), what they found was that warming, in and of itself,
was not a problem as long as the bias was gone. The big problem was
condensable contaminants that would be captured by the zeolites in the
dewar, released upon warming and then contaminating the SiLi crystal. They
were perfectly willing to ship those detectors warm, but the appendage pump
had a battery operated controller that shipped with it. Basically they were
far more concerned about the quality if the vacuum than the temperature of
the crystal.

We've all heard lots of stories about how one person's detector warmed up
once and was trashed, while others repeatedly warm their detectors and keep
right on truckin'. What I take from Kevex's experience is that it's kind of
a crap shoot and depends upon what is in the vacuum part of the dewar,
whether from manufacturing residues or from leaking and what condensables
might have entered through the leak(s).

The other slight risk with warming is that if you've had a bad leak for a
while, when the zeolites warm up, all the gasses they've captured are going
to be released, possibly going to considerable positive pressure and blowing
the window. It's probably not a concern unless your lN2 consumption is very
high, indicating a poor vacuum in the dewar, but thin windows make it more
of a concern than Be windows.

Is it PGT that made the LEAP detector? The dewar looks nothing like your
standard dewar, doesn't hold much lN2, and functions for years going warm
and cold, warm and cold. It may be that they got rid of the zeolites (hence
the sorption pumping) to eliminate the possibility of contaminating the
crystal upon warming. Maybe someone who has some info can comment on that.

I think the answer to your question is, "It's a crap-shoot." May the force
be with you.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Wednesday, February 25, 2009 2:22 PM
To: kenconverse-at-qualityimages.biz

This thread brought up a question.

Suppose someone wants to mothball a LN2 chilled detector for a period of
time.

Is this feasible without significant damage?

What precautions should be taken (bias off and voltage drained) prior
to warming up, etc?

What are the negative aspects of doing this (loss of resolution,
presumably)?

Thanks for the information.
--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original
Headers==============================
8, 19 -- From bozzola-at-siu.edu Wed Feb 25 13:19:55 2009
8, 19 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1PJJtov024308
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:55
-0600
8, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu
[131.230.177.136])
8, 19 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id
n1PJJrDS001332
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:54
-0600 (CST)
8, 19 -- Mime-Version: 1.0
8, 19 -- Message-Id: {a06240802c5cb492177ae-at-[131.230.177.136]}
8, 19 -- In-Reply-To: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- References: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
8, 19 -- Date: Wed, 25 Feb 2009 13:19:52 -0600
8, 19 -- To: Microscopy-at-microscopy.com
8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 19 -- Subject: [Microscopy] Another EDX detector question
8, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 19 -- X-Spam-Score: 0.00%
8, 19 -- X-MASF: 0.00%
8, 19 -- X-Whitelist: 0.00%
==============================End of -
Headers==============================




==============================Original
Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Wed Feb 25 13:59:15 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com
[75.180.132.122])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n1PJxEBq031361
25, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:59:14 -0600
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20090225195913.ZHOE20000.cdptpa-omta03.mail.rr.com-at-Ken} ;
25, 25 -- Wed, 25 Feb 2009 19:59:13 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: {bozzola-at-siu.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] Another EDX detector question
25, 25 -- Date: Wed, 25 Feb 2009 14:59:08 -0500
25, 25 -- Message-ID: {8769BB04EB5B45DCB461C784BBA81958-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="US-ASCII"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- Thread-Index: AcmXflHAu9xcsgraQs+YTApKmLuF6AAAX2TQ
25, 25 -- Importance: Normal
25, 25 -- In-Reply-To: {200902251921.n1PJLuQe028356-at-ns.microscopy.com}
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id
n1PJxEBq031361
==============================End of -
Headers==============================--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
38, 30 -- From r.sims-at-auckland.ac.nz Wed Feb 25 15:34:02 2009
38, 30 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
38, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PLXuKV019050
38, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Feb 2009 15:34:01 -0600
38, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
38, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1E0739D335;
38, 30 -- Thu, 26 Feb 2009 10:33:56 +1300 (NZDT)
38, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
38, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
38, 30 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
38, 30 -- with ESMTP id j-hcuw5rsZZC; Thu, 26 Feb 2009 10:33:55 +1300 (NZDT)
38, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
38, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
38, 30 -- (No client certificate requested)
38, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id E38E59D2FE;
38, 30 -- Thu, 26 Feb 2009 10:33:55 +1300 (NZDT)
38, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
38, 30 -- To: kenconverse-at-qualityimages.biz
38, 30 -- Date: Thu, 26 Feb 2009 10:36:01 +1300
38, 30 -- MIME-Version: 1.0
38, 30 -- Subject: Re: [Microscopy] RE: Another EDX detector question
38, 30 -- CC: Microscopy-at-Microscopy.Com
38, 30 -- Message-ID: {49A67091.4232.A5A7A7-at-r.sims.auckland.ac.nz}
38, 30 -- Priority: normal
38, 30 -- In-reply-to: {200902252000.n1PK0Jv7000820-at-ns.microscopy.com}
38, 30 -- References: {200902252000.n1PK0Jv7000820-at-ns.microscopy.com}
38, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
38, 30 -- Content-type: text/plain; charset=US-ASCII
38, 30 -- Content-transfer-encoding: 7BIT
38, 30 -- Content-description: Mail message body
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 25 Feb 2009 16:55:25 -0600
Subject: [Microscopy] Re: Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have three EDS systems in my lab, and several times I did let them go
to room temperature, each time unplugging them completely. No damage at
all.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy
}
}
} } Suppose someone wants to mothball a LN2 chilled detector for
} a period of time.
} } Is this feasible without significant damage?
} } What precautions should be taken (bias off and voltage
} drained) prior
} } to warming up, etc?
} } ?What are the negative aspects of doing this (loss of
} resolution, presumably)?
}
} Hi John
}
} I did this over a 1-month vacation period once, having been assured by

} the manufacturer that it was perfectly OK.
}
} The vacuum and resolution both deteriorated to the point of being
} unusable for quantitative work.
}
} After this had happened, and on further rather annoyed questioning,
} the manufacturer said that some deterioration in performance was to be

} expected.
}
} I chose to replace it, with one from a different maker!
}
} I will never, never do this again unless it is 100% unavoidable.
}
} cheers
}
} Ritchie
}
}
}
}
} --
} Ritchie Sims Ph D Phone : 64 9
} 3737599 ext 87713
} Microanalyst Fax :
} 64 9 3737435
} Department of Geology email :
} r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
} ==============================Original
} Headers==============================
} 15, 30 -- From r.sims-at-auckland.ac.nz Wed Feb 25 13:49:26 2009 15, 30
} -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz
} [130.216.12.34])
} 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n1PJnP3r020068
} 15, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Feb
} 2009 13:49:26 -0600
} 15, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP
} id A4D551A2C9;
} 15, 30 -- Thu, 26 Feb 2009 08:49:24 +1300 (NZDT)
} 15, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
} 15, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
} 15, 30 -- by localhost (larry.its.auckland.ac.nz
} [127.0.0.1]) (amavisd-new, port 10024)
} 15, 30 -- with ESMTP id R01SrwJsNjjA; Thu, 26 Feb 2009
} 08:49:24 +1300 (NZDT)
} 15, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz
} [130.216.59.18])
} 15, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
} 15, 30 -- (No client certificate requested)
} 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP
} id 7ED311A284;
} 15, 30 -- Thu, 26 Feb 2009 08:49:23 +1300 (NZDT)
} 15, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 30
} -- To: bozzola-at-siu.edu 15, 30 -- Date: Thu, 26 Feb 2009
} 08:51:29 +1300 15, 30 -- MIME-Version: 1.0 15, 30 -- Subject:
} Re: [Microscopy] Another EDX detector question 15, 30 -- CC:
} Microscopy-at-Microscopy.Com 15, 30 -- Message-ID:
} {49A65811.27551.45F397-at-r.sims.auckland.ac.nz}
} 15, 30 -- Priority: normal
} 15, 30 -- In-reply-to: {200902251920.n1PJKcS4025632-at-ns.microscopy.com}
} 15, 30 -- References: {200902251920.n1PJKcS4025632-at-ns.microscopy.com}
} 15, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 30 --
} Content-type: text/plain; charset=US-ASCII 15, 30 --
} Content-transfer-encoding: 7BIT 15, 30 --
} Content-description: Mail message body
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 23 -- From DusevichV-at-umkc.edu Wed Feb 25 16:55:24 2009
7, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PMtNDc002882
7, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 16:55:24 -0600
7, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 23 -- Wed, 25 Feb 2009 16:55:23 -0600
7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 23 -- Content-class: urn:content-classes:message
7, 23 -- MIME-Version: 1.0
7, 23 -- Content-Type: text/plain;
7, 23 -- charset="US-ASCII"
7, 23 -- Subject: Re: Another EDX detector question
7, 23 -- Date: Wed, 25 Feb 2009 16:55:22 -0600
7, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7E0-at-KC-MSX1.kc.umkc.edu}
7, 23 -- X-MS-Has-Attach:
7, 23 -- X-MS-TNEF-Correlator:
7, 23 -- Thread-Topic: Re: Another EDX detector question
7, 23 -- thread-index: AcmXgkvTxziBaRRYTMuSR9KTxkwD0wAFF3NwAAFZkWA=
7, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 23 -- To: {Microscopy-at-microscopy.com}
7, 23 -- X-OriginalArrivalTime: 25 Feb 2009 22:55:23.0233 (UTC) FILETIME=[240D8D10:01C9979C]
7, 23 -- Content-Transfer-Encoding: 8bit
7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1PMtNDc002882
==============================End of - Headers==============================




From: cannonmp-at-comcast.net
Date: Wed, 25 Feb 2009 19:33:51 -0600
Subject: [Microscopy] viaWWW: EDS Detectors and Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cannonmp-at-comcast.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cannonmp-at-comcast.net
Name: Bart Cannon

Organization: Cannon Microprobe

Title-Subject: [Filtered] EDS Detectors and Dewars

Question:
I thought that my experiences with SiLi x-ray detectors and dewars might be
of interest.

I have a 1980 Kevex EDS detector. It had been at room temp for six years
when I bought it in 1990. I dismantled the cryo-tip and extended it and its
vacuum enclosure by nearly a foot in order to accomodate an extreme geometry
on my ARL SEMQ electron probe.

At the same time, I constructed some valving and tubulation in order to make
the dewar vacuum continuous with my system vacuum when a valve was opened.

Since 1990 I have routinely let my dewar go dry several times a week. I
only add enough LN2 to complete a day's work. When the dewar goes dry, I
pump it down.

Last year there was a five day period during which my dewar went dry and I
had left the bias on (-640 volts). I felt the end had come, but I pumped
down the system and added LN2. Everything was fine and my 29 year old
detector was back to better than 160 ev resolution at Mn ka.

I have a friend with detector experience. His comment was that I have one
of the good ones.

Bart Cannon
Cannon Microprobe



Login Host: 206.69.208.22
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Wed Feb 25 19:33:51 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q1Xms6021218
14, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 19:33:50 -0600
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240804c5cba1cc4d61-at-[206.69.208.22]}
14, 11 -- Date: Wed, 25 Feb 2009 19:33:47 -0600
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: cannonmp-at-comcast.net (by way of Nestor J. Zaluzec)
14, 11 -- Subject: viaWWW: EDS Detectors and Dewars
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 25 Feb 2009 19:53:44 -0600
Subject: [Microscopy] Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I second Vladimir's experience. I had used an EDAX
Sapphire Dewar 204 detector and let it sit at room
temperature for weeks. No problem. SafeFill was
turned on when the detector was needed--it loads
the LN2. Again, no problem.

Nowadays, the EDAX Apollo 40 SDD makes a huge difference.
I figure that all or most SDD maker's SDD systems will
be vastly superior to legacy Si(Li) EDS detectors.

There are argumentative issues with SDD specs but the
fact remains, IMO, that SDD will kill Si(Li) and LN2
systems over time. The current generation of SDD is
too phenomenal to dismiss. Money is always an issue.
ROI and up-time are also factors. The SDD in and of
itself was evolutionary and now it appears to me to
be revolutionary with what I think is the third generation
of SDD detector chips. The differences from Si(Li) are
stunning!

As a side note, the EDAX Apollo 40 has a small ion pump
to maintain vacuum. This is very nice. I had suggested
this some time ago for the Si(Li) flavors of detectors.
I have no idea if they did this based on my suggestion.
But they did do it. Thus, the getters issue is gone.
This was an issue with non-LN2 detectors...old history.

DISCLAIMER: I have no financial interest in EDAX/Ametek/TSL
other than being a very satisfied customer of their products
and service.

gary g.




At 02:57 PM 2/25/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
14, 20 -- From gary-at-gaugler.com Wed Feb 25 19:53:44 2009
14, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1Q1rh8b002917
14, 20 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 19:53:43 -0600
14, 20 -- Message-Id: {200902260153.n1Q1rh8b002917-at-ns.microscopy.com}
14, 20 -- Received: (qmail 26504 invoked from network); 25 Feb 2009 17:52:04 -0800
14, 20 -- Received: by simscan 1.1.0 ppid: 26500, pid: 26502, t: 0.1716s
14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
14, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
14, 20 -- by smtp2 with SMTP; 25 Feb 2009 17:52:04 -0800
14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 20 -- Date: Wed, 25 Feb 2009 17:53:38 -0800
14, 20 -- To: DusevichV-at-umkc.edu
14, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
14, 20 -- Subject: Re: [Microscopy] Re: Another EDX detector question
14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
14, 20 -- In-Reply-To: {200902252257.n1PMvE4j005317-at-ns.microscopy.com}
14, 20 -- References: {200902252257.n1PMvE4j005317-at-ns.microscopy.com}
14, 20 -- Mime-Version: 1.0
14, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gerd.leitinger-at-medunigraz.at
Date: Thu, 26 Feb 2009 02:14:12 -0600
Subject: [Microscopy] TEM: Membrane proteins in post-embedding immunogold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all:

I have a question concerning immunogold labelling of membrane proteins on tissue embedded in LR White:
We labelled a positive control - a cytoplasmatic protein - on muscle tissue embedded in LR White and tested two antibodies against membrane proteins.

The positive control worked extremely well, but the two membrane proteins do not show such a clear labelling- it is useful but there is a lot of background and unspecific labelling in the nucleoplasm.

Is it possible that the membranes are partially washed out or effected by the dehydration process?

Which procedure do you recommend (pre-embedding? or post- embedding with special dehydration steps?) for labelling membrane proteins?

thank you

Gerd


--
Dr. Gerd Leitinger

Laboratory Coordinator
Core Facility Ultrastructure Analysis
Center for Medical Research (ZMF)
Medical University of Graz

Postal address:
Institute of Cell Biology, Histology and Embryology
Harrachgasse 21
8010 Graz
Austria
Tel. +43 316 380 4237
Fax. +43 316 380 9625
Mailto: Gerd.Leitinger-at-medunigraz.at




==============================Original Headers==============================
12, 22 -- From gerd.leitinger-at-medunigraz.at Thu Feb 26 02:14:12 2009
12, 22 -- Received: from si069.medunigraz.at (si082.meduni-graz.at [193.170.105.82])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q8ECKj028517
12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 02:14:12 -0600
12, 22 -- X-AuditID: 0ac80c45-aa347bb000000f5d-b2-49a64f545bca
12, 22 -- Received: from si062.medunigraz.at (si062.meduni-graz.at [10.200.12.62])
12, 22 -- by si069.medunigraz.at (Symantec Mail Security) with ESMTP id 3ADDF4DC002
12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 09:14:12 +0100 (CET)
12, 22 -- Received: from connect_62-MTA by si062.medunigraz.at
12, 22 -- with Novell_GroupWise; Thu, 26 Feb 2009 09:14:12 +0100
12, 22 -- Message-Id: {49A65D59.E434.00B0.0-at-medunigraz.at}
12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
12, 22 -- Date: Thu, 26 Feb 2009 09:13:53 +0100
12, 22 -- From: "Gerd Leitinger" {gerd.leitinger-at-medunigraz.at}
12, 22 -- To: {Microscopy-at-microscopy.com}
12, 22 -- Subject: TEM: Membrane proteins in post-embedding immunogold
12, 22 -- Mime-Version: 1.0
12, 22 -- Content-Type: text/plain; charset=US-ASCII
12, 22 -- Content-Disposition: inline
12, 22 -- X-Brightmail-Tracker: AAAAAA==
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1Q8ECKj028517
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Thu, 26 Feb 2009 02:39:00 -0600
Subject: [Microscopy] Re: TEM: Membrane proteins in post-embedding immunogold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gerd,

It has been reported that membrane proteins can be extracted from
membranes, mechanically, although I can not recall any proteins having
been washed out. Certainly they may be affected by fixation and
dehydration.
Which approach? There are many approaches that can get good results in
immunolabeling, but I think first you might want to look at your
results as they are and try to understand where signal and background
originate from.
Can you describe the species of primary antibodies and what kind of
gold conjugate (protein A.. secondary antibody) you used in both
cases? What are the negative controls like, i.e. the ones without
primary?
What source (species) was the tissue from?


Jan Leunissen

Aurion
http://www.aurion.nl


On 26/02/2009, at 9:14 PM, gerd.leitinger-at-medunigraz.at wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear all:
}
} I have a question concerning immunogold labelling of membrane
} proteins on tissue embedded in LR White:
} We labelled a positive control - a cytoplasmatic protein - on muscle
} tissue embedded in LR White and tested two antibodies against
} membrane proteins.
}
} The positive control worked extremely well, but the two membrane
} proteins do not show such a clear labelling- it is useful but there
} is a lot of background and unspecific labelling in the nucleoplasm.
}
} Is it possible that the membranes are partially washed out or
} effected by the dehydration process?
}
} Which procedure do you recommend (pre-embedding? or post- embedding
} with special dehydration steps?) for labelling membrane proteins?
}
} thank you
}
} Gerd
}
}
} --
} Dr. Gerd Leitinger
}
} Laboratory Coordinator
} Core Facility Ultrastructure Analysis
} Center for Medical Research (ZMF)
} Medical University of Graz
}
} Postal address:
} Institute of Cell Biology, Histology and Embryology
} Harrachgasse 21
} 8010 Graz
} Austria
} Tel. +43 316 380 4237
} Fax. +43 316 380 9625
} Mailto: Gerd.Leitinger-at-medunigraz.at
}
}
}
}
} ==============================Original
} Headers==============================
} 12, 22 -- From gerd.leitinger-at-medunigraz.at Thu Feb 26 02:14:12 2009
} 12, 22 -- Received: from si069.medunigraz.at (si082.meduni-graz.at
} [193.170.105.82])
} 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n1Q8ECKj028517
} 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009
} 02:14:12 -0600
} 12, 22 -- X-AuditID: 0ac80c45-aa347bb000000f5d-b2-49a64f545bca
} 12, 22 -- Received: from si062.medunigraz.at (si062.meduni-graz.at
} [10.200.12.62])
} 12, 22 -- by si069.medunigraz.at (Symantec Mail Security) with
} ESMTP id 3ADDF4DC002
} 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009
} 09:14:12 +0100 (CET)
} 12, 22 -- Received: from connect_62-MTA by si062.medunigraz.at
} 12, 22 -- with Novell_GroupWise; Thu, 26 Feb 2009 09:14:12 +0100
} 12, 22 -- Message-Id: {49A65D59.E434.00B0.0-at-medunigraz.at}
} 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
} 12, 22 -- Date: Thu, 26 Feb 2009 09:13:53 +0100
} 12, 22 -- From: "Gerd Leitinger" {gerd.leitinger-at-medunigraz.at}
} 12, 22 -- To: {Microscopy-at-microscopy.com}
} 12, 22 -- Subject: TEM: Membrane proteins in post-embedding immunogold
} 12, 22 -- Mime-Version: 1.0
} 12, 22 -- Content-Type: text/plain; charset=US-ASCII
} 12, 22 -- Content-Disposition: inline
} 12, 22 -- X-Brightmail-Tracker: AAAAAA==
} 12, 22 -- Content-Transfer-Encoding: 8bit
} 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n1Q8ECKj028517
} ==============================End of -
} Headers==============================




==============================Original Headers==============================
11, 19 -- From leunissen-at-aurion.nl Thu Feb 26 02:38:59 2009
11, 19 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q8cv7S010253
11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 02:38:58 -0600
11, 19 -- Received: from [192.168.1.50] (really [122.57.247.59]) by fep01.xtra.co.nz
11, 19 -- with ESMTP
11, 19 -- id {20090226083851.HNVO7189.fep01.xtra.co.nz-at-[192.168.1.50]}
11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:38:51 +1300
11, 19 -- Message-Id: {6546CB94-4E4D-4AFF-AED2-3FEACEBC939D-at-aurion.nl}
11, 19 -- From: Jan Leunissen {leunissen-at-aurion.nl}
11, 19 -- To: microscopy-at-microscopy.com
11, 19 -- In-Reply-To: {200902260814.n1Q8EVGe028864-at-ns.microscopy.com}
11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 19 -- Subject: Re: [Microscopy] TEM: Membrane proteins in post-embedding immunogold
11, 19 -- Date: Thu, 26 Feb 2009 21:38:50 +1300
11, 19 -- References: {200902260814.n1Q8EVGe028864-at-ns.microscopy.com}
11, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Thu, 26 Feb 2009 03:58:52 -0600
Subject: [Microscopy] Re: Epon/PAS stain ...?? (Sorry for longness)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Note Added by Wolfgang M.: [ cited right now from most recently done search via PubMed: Microsc Acta. 1980 May;83(2):111-6. ]
Predecessor of that journal was:
MICROSCOPICA ACTA , journal of microscopic equipment, methods and applications Stuttgart(Germany): Hirzel Verlag; Vorgänger[predecessor]: Zeitschrift fuer wissenschaftliche Mikroskopie und mikroskopische Technik
(==}
http://www2.hu-berlin.de/IGAFA/cgi-bin/js0730.cgi?equ=a&search=Zeitschrift_für_wissenschaftliche_Mikroskopie_und_mikroskopische_Technik ).
This Journal unfortunately neither has been published online nor does have a successor title including that particular volume/issue (but see below: Micron = sucsessor /merger of Micron AND Microscopica acta).
Perhaps in an excellent, old fashioned big library you will get the particular issue /article.

BUT - cited also this way in PubMed, unfortunately, the bibliographic data given by Dieter Bosshart and Pub Med seem to be wrong(?), because no such "high" volume No of the online- } Micron and Microscopica Acta { ever has been printed...
Cf:
PubMed: searching for } Microscop Acta. { you will find that the last Vol/Issue No available is:
Microsc Acta. 1983 May;87(3) so it seems that the production/publication of the particular journal has been discontinued in 1983(see below).
If you search PubMed for } Microsc Acta. { you'll get 353 , if you search for
} Microscopica Acta { you'll get 462 results.
Definition by PubMed/NIH/NLM ID:
Abbrev: Microsc Acta
ISSN: 0044-376X (Print) Title Abbreviation: Microsc Acta Publication Start Year: 1971 Publication End Year: 1983 Current Indexing Status: Not currently indexed for MEDLINE. Version Currently Indexed: Print Publisher: S Hirzel Verlag Continuation Notes: Continues Zeitschrift für Wissenschaftliche Mikroskopie und mikroskopische Technik. Merged with {Micron to form Micron and microscopica acta} . Language: English, German Place of Publication: Germany Subject Term(s): Diagnostic Imaging
NLM ID: 1306037 {http://www.ncbi.nlm.nih.gov/sites/entrez?Db=nlmcatalog&doptcmdl=Expanded&cmd=search&Term=1306037[NlmId]}
Unfortunately the "publication history" is a little bit tricky here,
cf. PubMed, search phrase: 9312850[NlmId] (= } Micron {) , searchphrase: "Micron and microscopia acta"[TITLE] NOT 9312850[NLM Unique ID] : no items found,
BUT:
Micron online (= successor of "Micron & Micr.Acta") see: http://www.sciencedirect.com/science/journal/09684328
Copyright © 2009 Elsevier Ltd. All rights reserved:
Publication History: Incorporating Electron Microscopy Reviews {/science/journal/08920354} and Micron and Microscopica Acta {/science/journal/07396260}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {

Apologies:
Due to some problems in connecting with the MSA-Listserver-forum duringe the last 2 days this my message comes a little bit late...


Good morning,

Dear Anne-Marie Brun,
your quest (at least for me) is likely a very special one.

It might be wise to tell your "investigator" that he is asking for your doing "extravagant work" to please him.

Needless to remind that PAS staining (I mean "real PAS- = } periodic acid -Schiff-reaction- { Staining" like done in histochemistry on paraffin or cryosections) IS NOT POSSIBLE to facilitate ON regular EPON / EPOXY SECTIONS (with some exceptions, provided that initially blocking of (free) aldehydes after fixation has been done).

This on the one hand is due to the primary/secondary fixation usually used in electron microscopic specimen preparations (i.e. FA [formaldehyde]-GA, GA=[glutaraldehyde] fixation, OsO4-postfix) as well as on the other hand due to the unfavorable properties of the resin used: hydrophobic [and firmly polymerized] epoxid/Epon.

In routine TEM-processing, after the primary fixation as done usually, nobody (perhaps only somebody) will block free aldehydes (which are left from FA-GA-fixation) in the tissue after fixation (e.g. by Na-(sodium-)borohydride-solution, or PBS containing 50 mM glycine , or dimedone, or IMO - better/best - 0.05M NH4Cl = ammoniumchloride in 0.1 M washing buffer 10-25 min at Room-Temp) on a routine basis (some -many?- do it when immunolabelling IEM is the final task).

Without using "Aldehyde-blocking" solutions you will get "false-positive" results of PAS staining.

Exceptions for realizing "PAS"-staining on 100% EPON-/solely epoxy-resin sections (perhaps/certainly my opinion(s), not exhaustive) are:

- Etching of resin prior to staining (e.g. sodium(Na)or potassium (K)-ethylate-solution, and other special "ripened" solvents) certainly is necessary / a prerequisite for.

There are a lot of (old and older) papers/articles on that [mostly 1970ies/1980ies] which not only were published because of demands in finding "real" classical histochemical stainings on (epoxy) resin sections but also due to the advent of "immuno...." applications in (T)EM...this was before the advent of } hydro {philic acrylic resins like LR WHITE, LR GOLD, UNICRYL, and the LOWICRYLS.

Some (many old) publications deal with the routine use of resin combinations like epon - araldite...which supposedly (and in hands of "specialists", who certainly will catch the speaker's eye in this MSA thread most sucessfully) provide almost true "histochemical" stainings (also after "etching" sections or block faces).

- Use of methacrylate resin embedded tissue: for this task I can provide you with a recipe out from the famous (but exhausted/out of print) booklet of BURNS and BRETSCHNEIDER (1981) - Thin is in: Plastic Embedding of Tissue for Light Microscopy....

- and, last but not least you might fail also due to using not the correct staining solutions.... (hydrophobic as well as hydrophilic resins at large perhaps need other formulae or application steps than formerly paraffin-embedded or cryo-preserved material sections handled for LM-histochemistry.

In 2006 there was some traffic on the MSA-Listserver (and I am confident that in the ARCHIVES a lot of requests deal with the question "PAS-Staining of resin sections") and ONE good reply was (by Dieter D. Bosshardt, Switzerland):

{ { Some years ago, I did PAS staining of Epon sections (0.5-1.0 micron thick). There is a good reference explaining the procedure:
Schroeder HE, Rossinsky K, Müller W (1980) An established routine method for differential staining of epoxy-embedded tissue sections. } {Microscopica Acta} 83,111-116.
Hope this helps.
Best regards, Dieter
--
Dieter D. Bosshardt, Ph.D.
University of Bern
School of Dental Medicine
Department of Periodontology & Fixed Prosthodontics
Post Box 64
Freiburgstrasse 7
CH-3010 Bern 10
Switzerland
http://dent.unibe.ch
Phone: +41-31-632 86 05
Fax: +41-31-632 49 31
mailto:dieter.bosshardt-at-zmk.unibe.ch

=======================================================================================================

For now, I stop here and shall see what other listers will offer...and meanwhile I shall try to get a reprint of the article mentioned above....

Best wishes and regards,

Wolfgang Muss

OR Dr. phil. Wolfgang Muss
Head of EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively (same Lab, same address)

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {

-------------------------------------------------------------------------
Forthcoming in 2009:
+++2009, June 11th - June 13th: 36th Ann. SCUR Meeting (SCUR meets Florence)+++
Host: Francesca Prignano and her team
We cordially invite you to participate actively in the meeting.
Visit: Next Meetings at:
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/index_ger.html

FIRST ANNOUNCEMENT:
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/e1003/SCUR2009_First_Announcement.pdf
(PDF 650KB)

(Call for papers and detailed informations for Registration and Paper Submission to be included on this website as early as possible).
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/e772/index_ger.html




} -----Ursprüngliche Nachricht-----
} Von: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu]
} Gesendet: Mittwoch, 25. Februar 2009 04:36
} An: Muß Wolfgang
} Betreff: [Microscopy] Epon/PAS stain ...??
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------
} This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber,so when replying
} please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
} Email: abrun-at-hsc.unt.edu
} Name: Anne-Marie Brun
} Organization: UNT HSC at Fort Worth Texas 76107, USA
} Title-Subject:
} Epon/PAS stain
} Question:
}
} Has anyone ever stained for PAS on Epon sections before?
}
} If not on Epon then what type of plastic did you use to do a
} PAS stain?
} I know it is done on paraffin embedded material, but an
} investigator wants the PAS staining done on Epon sections.
}
} ----?
} Thanks for your help
} Anne-Marie
}
} Login Host: 129.120.96.228
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:32 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n1P3RVv3007908
} 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb
} 2009 21:27:31 -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240800c5ca6b1378b7-at-[206.69.208.22]}
} 6, 11 -- Date: Tue, 24 Feb 2009 21:27:29 -0600
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: Epon/PAS stain
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of Headers==============================
}



==============================Original Headers==============================
35, 35 -- From W.Muss-at-salk.at Thu Feb 26 03:58:51 2009
35, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
35, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q9wooi026478
35, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 03:58:50 -0600
35, 35 -- Received: from localhost (localhost [127.0.0.1])
35, 35 -- by hermes.salk.at (Postfix) with ESMTP id F194FC386E;
35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100 (CET)
35, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
35, 35 -- Received: from hermes.salk.at ([127.0.0.1])
35, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
35, 35 -- with ESMTP id kD7SJvbS6wAL; Thu, 26 Feb 2009 10:58:48 +0100 (CET)
35, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
35, 35 -- by hermes.salk.at (Postfix) with ESMTP id 7E876C383D;
35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100 (CET)
35, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100
35, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
35, 35 -- Content-class: urn:content-classes:message
35, 35 -- MIME-Version: 1.0
35, 35 -- Content-Type: text/plain;
35, 35 -- charset="iso-8859-1"
35, 35 -- Subject: [Microscopy] Re: Epon/PAS stain ...?? (Sorry for longness)
35, 35 -- Date: Thu, 26 Feb 2009 10:58:48 +0100
35, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0881-at-N1RZ116.lksdom21.lks.local}
35, 35 -- X-MS-Has-Attach:
35, 35 -- X-MS-TNEF-Correlator:
35, 35 -- Thread-Topic: [Microscopy] Re: Epon/PAS stain ...?? (Sorry for longness)
35, 35 -- Thread-Index: AcmX+NG+gYN9Cap5S3uzXxIql6UFOg==
35, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
35, 35 -- To: {microscopy-at-microscopy.com} , {wij.muss-at-aon.at} ,
35, 35 -- =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
35, 35 -- X-OriginalArrivalTime: 26 Feb 2009 09:58:48.0642 (UTC) FILETIME=[D1ECF220:01C997F8]
35, 35 -- X-Scanned-By: SALK-Content-Filter
35, 35 -- Content-Transfer-Encoding: 8bit
35, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1Q9wooi026478
==============================End of - Headers==============================




From: Karen.Fleming-at-jhu.edu
Date: Thu, 26 Feb 2009 08:30:21 -0600
Subject: [Microscopy] viaWWW: Attaching a camera to a Nikon SMZ-2T phototube

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Karen.Fleming-at-jhu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Karen.Fleming-at-jhu.edu
Name: Karen Fleming

Organization: Johns Hopkins

Title-Subject: [Filtered] Attaching a camera to a Nikon SMZ-2T phototube

Question: Does anyone have an older SMZ-2T stereo microscope with a
coolpix camera setup on the phototube? The camera on ours was stolen
a few years ago, and I'm trying to figure out what I need to restore
picture taking abilities. I minimally want to achieve lab-notebook
quality pictures of protein crystals. This is for a new class I'm
teaching and of course there's no real budget for purchasing a whole
new setup, so I'm trying to find replacement parts at minimal cost.

What remains on the microscope is the phototube itself, which I think
has a C-mount male thread on the top. This is a metal beige piece
that I think is the V-T phototube, and it is removable.

Inside the phototube is a removable TV lens (1X/16).

The old camera was a Nikon Coolpix 990 with a 28mm thread. It seems
pretty straightforward to purchase a used camera on Ebay, but from
extensively consulting Mr. Google, I think I'm also missing a relay
lens that is supposed to go between the camera and the phototube. The
lens I think I might be missing is the MDC-A relay lens, which is no
longer being made by Nikon

Can anyone confirm this thinking? I'm not exactly sure that the
phototube is a C-mount, but I do know that it has male threads I
can't screw 28mm threads directly onto it. The threads on the
potential c-mount seem more widely spread than the threads on a
female 28mm camera filter.

If this is a C-mount does anyone know if the "Nikon camera lens
adaptor for C-mount microscope" item on ebay would be an appropriate
MDC-A substitute.
http://cgi.ebay.com/NIKON-CAMERA-LENS-ADAPTER-FOR-C-MOUNT-MICROSCOPE_W0QQitemZ250378986591QQcmdZViewItemQQptZLH_DefaultDomain_0?hash=item250378986591&_trksid=p3286.c0.m14&_trkparms=72%3A1205|66%3A2|65%3A12|39%3A1|240%3A1318|301%3A1|293%3A1|294%3A50


Login Host: 69.138.189.238
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Thu Feb 26 08:30:20 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1QEUKCU018660
12, 11 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 08:30:20 -0600
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240801c5cc57ea7f22-at-[206.69.208.22]}
12, 11 -- Date: Thu, 26 Feb 2009 08:30:16 -0600
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: Karen.Fleming-at-jhu.edu (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Attaching a camera to a Nikon SMZ-2T phototube
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Thu, 26 Feb 2009 11:30:32 -0600
Subject: [Microscopy] Phase telescope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an Olympus centering telescope for a BH-2 phase contrast
microscope that has frozen. Has anyone had any luck getting one of these
un-stuck?

Thanks,

Andy Bowling


==============================Original Headers==============================
4, 31 -- From AJBowling-at-dow.com Thu Feb 26 11:30:32 2009
4, 31 -- Received: from mail164.messagelabs.com (mail164.messagelabs.com [216.82.253.131])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1QHUViJ011530
4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 11:30:31 -0600
4, 31 -- X-VirusChecked: Checked
4, 31 -- X-Env-Sender: AJBowling-at-dow.com
4, 31 -- X-Msg-Ref: server-4.tower-164.messagelabs.com!1235669425!16997483!5
4, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,-
4, 31 -- X-Originating-IP: [204.136.184.21]
4, 31 -- Received: (qmail 12635 invoked from network); 26 Feb 2009 17:30:30 -0000
4, 31 -- Received: from mail3.dow.com (HELO USFRPMDOWS001.dow.com) (204.136.184.21)
4, 31 -- by server-4.tower-164.messagelabs.com with RC4-SHA encrypted SMTP; 26 Feb 2009 17:30:30 -0000
4, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USFRPMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830);
4, 31 -- Thu, 26 Feb 2009 11:30:27 -0600
4, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
4, 31 -- Content-class: urn:content-classes:message
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- charset="us-ascii"
4, 31 -- Subject: Phase telescope
4, 31 -- Date: Thu, 26 Feb 2009 12:30:26 -0500
4, 31 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1DA6474-at-USMDLMDOWX032.dow.com}
4, 31 -- X-MS-Has-Attach:
4, 31 -- X-MS-TNEF-Correlator:
4, 31 -- Thread-Topic: Phase telescope
4, 31 -- Thread-Index: AcmYN+lCKx0Et6jfSY2nPb6qKQH1GA==
4, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
4, 31 -- To: {microscopy-at-microscopy.com}
4, 31 -- X-OriginalArrivalTime: 26 Feb 2009 17:30:27.0673 (UTC) FILETIME=[EA34EC90:01C99837]
4, 31 -- Content-Transfer-Encoding: 8bit
4, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1QHUViJ011530
==============================End of - Headers==============================




From: maloneyb-at-fiu.edu
Date: Thu, 26 Feb 2009 13:36:06 -0600
Subject: [Microscopy] ISI DS130 operating manual and/or schematics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group: would anyone by chance of a copy of this SEM's manual?
Thanks so much
Barbara


==============================Original Headers==============================
2, 22 -- From maloneyb-at-fiu.edu Thu Feb 26 13:36:06 2009
2, 22 -- Received: from fiumailsmtp.fiu.edu (fiuht01.ad.fiu.edu [131.94.75.139])
2, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1QJa5i5030575
2, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 13:36:05 -0600
2, 22 -- Received: from FIUMB02CCR.ad.fiu.edu ([192.168.152.35]) by fiuht01.ad.fiu.edu
2, 22 -- ([192.168.251.10]) with mapi; Thu, 26 Feb 2009 14:36:02 -0500
2, 22 -- From: Barbara Maloney {maloneyb-at-fiu.edu}
2, 22 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
2, 22 -- Date: Thu, 26 Feb 2009 14:33:13 -0500
2, 22 -- Subject: ISI DS130 operating manual and/or schematics
2, 22 -- Thread-Topic: ISI DS130 operating manual and/or schematics
2, 22 -- Thread-Index: AQHJmEl1JJBiC/Exrk6YC3MJp085Pw==
2, 22 -- Message-ID: {C4B460B46F1B5E42A09CA563ECD4DE530810D97CC3-at-FIUMB02CCR.ad.fiu.edu}
2, 22 -- Accept-Language: en-US
2, 22 -- Content-Language: en-US
2, 22 -- X-MS-Has-Attach:
2, 22 -- X-MS-TNEF-Correlator:
2, 22 -- acceptlanguage: en-US
2, 22 -- Content-Type: text/plain; charset="us-ascii"
2, 22 -- MIME-Version: 1.0
2, 22 -- Content-Transfer-Encoding: 8bit
2, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1QJa5i5030575
==============================End of - Headers==============================




From: ekanayake.a-at-pg.com
Date: Thu, 26 Feb 2009 19:57:00 -0600
Subject: [Microscopy] viaWWW: Images of plant cell walls & protein bodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ekanayake.a-at-pg.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ekanayake.a-at-pg.com
Name: Athula Ekanayake

Organization: Procter & Gamble

Title-Subject: [Filtered] Images of plant cell walls & protein bodies

Question: I am teaching a class on food proteins to Culinary school
undergrads and want to show some SEM images of protein bodies in
plant cells.
Do you have any suggetsions for a good website to get some clean images.

thanks

Login Host: 192.44.136.103
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Feb 26 19:57:00 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1R1uxWM024231
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 19:56:59 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c5ccf8d536ee-at-[206.69.208.22]}
7, 11 -- Date: Thu, 26 Feb 2009 19:56:56 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ekanayake.a-at-pg.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Images of plant cell walls & protein bodies
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Hobie-at-technicalsalessolutions.com
Date: Thu, 26 Feb 2009 21:17:15 -0600
Subject: [Microscopy] Cryo Stage for Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List Servers-

I will swap you a cold stage for a Hitachi SEM in exchange for a donation to
the Valley Catholic HS EM Lab.

See image of cryo stage using the following link (SEM is not available):

http://www.technicalsalessolutions.com/Instruments/SEM/Hitachi%20S-2300%20C
ryo.htm

I will email additional images of the stage upon request.

Thank you,

Hobie

Hobie Richards
Partner, and COO
Technical Sales Solutions, LLC
Portland, OR USA
www.TechnicalSalesSolutions.com
503 781 0428

Skype Hobie-TSS




==============================Original Headers==============================
12, 19 -- From Hobie-at-technicalsalessolutions.com Thu Feb 26 21:17:15 2009
12, 19 -- Received: from host203.com (host203.com [203.194.159.243])
12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1R3HDqG007223
12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:17:14 -0600
12, 19 -- Received: (qmail 557 invoked by uid 503); 27 Feb 2009 03:17:11 -0000
12, 19 -- Received: from unknown (HELO ?10.0.1.195?) (Hobie-at-71.236.222.78)
12, 19 -- by host203.com with ESMTPA; 27 Feb 2009 03:17:11 -0000
12, 19 -- User-Agent: Microsoft-Entourage/12.0.0.071130
12, 19 -- Date: Thu, 26 Feb 2009 19:17:09 -0800
12, 19 -- Subject: Cryo Stage for Hitachi SEM
12, 19 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com}
12, 19 -- To: {microscopy-at-microscopy.com}
12, 19 -- Message-ID: {C5CC9B35.175D5%Hobie-at-technicalsalessolutions.com}
12, 19 -- Thread-Topic: Cryo Stage for Hitachi SEM
12, 19 -- Thread-Index: AcmYid/V+68fSI09z0K/vOEZCgcpIw==
12, 19 -- Mime-version: 1.0
12, 19 -- Content-type: text/plain;
12, 19 -- charset="US-ASCII"
12, 19 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 26 Feb 2009 21:42:47 -0600
Subject: [Microscopy] Re: Phase telescope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've had this happen on old BH focusing telescopes.
The juice/oil...whatever solidifies over time.

This probably sounds ludicrous, but if you heat
the unit in an over to about 100F, usually the
stuck goo will let go. The key is to release the
goo without damaging the optics.

The common solvent for the good seems to be acetone.
But, in an heated environment, it will be gone quickly.
So, you might have to try both approaches. Heat first
and try to release then introduce acetone. Once released,
put new oil on the threads. I have found that the best
oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000
Clock/Watchmaker 1045.

This little bottle will last a lifetime. I have no idea if
this is still available. If you cannot get it, I can send a
few drops of this to you and that should serve you well
into the future.

gary g.


At 09:31 AM 2/26/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009
11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1R3giiv021379
11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:42:45 -0600
11, 20 -- Message-Id: {200902270342.n1R3giiv021379-at-ns.microscopy.com}
11, 20 -- Received: (qmail 14792 invoked from network); 26 Feb 2009 19:46:27 -0800
11, 20 -- Received: by simscan 1.1.0 ppid: 14789, pid: 14790, t: 0.1064s
11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 20 -- by smtp1 with SMTP; 26 Feb 2009 19:46:27 -0800
11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 20 -- Date: Thu, 26 Feb 2009 19:42:34 -0800
11, 20 -- To: AJBowling-at-dow.com
11, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 20 -- Subject: Re: [Microscopy] Phase telescope
11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 20 -- In-Reply-To: {200902261731.n1QHVwG6013931-at-ns.microscopy.com}
11, 20 -- References: {200902261731.n1QHVwG6013931-at-ns.microscopy.com}
11, 20 -- Mime-Version: 1.0
11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Fri, 27 Feb 2009 14:05:31 -0600
Subject: [Microscopy] Re: Phase telescope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ok, so I put the telescope in the oven at ~100F for an hour, just as
Gary suggested, and this worked like a charm! I unscrewed it enough to
expose some threads and I put a drop of Liquid Wrench with Teflon on
them, worked it in a bit, and wiped off the liberated green goo with a
kimwipe (I repeated this a few times). I tested it again after putting
it in the fridge for a while to make sure that the grease wouldn't set
when it cooled down, and it appears to be totally fixed. One final note,
I had already tried to heat the telescope in the same oven set at 60
degrees and it didn't work. Heating it higher was definitely required.

Thanks!

Andy Bowling

-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Thursday, February 26, 2009 10:49 PM
To: Bowling, Andrew (AJ)

I've had this happen on old BH focusing telescopes.
The juice/oil...whatever solidifies over time.

This probably sounds ludicrous, but if you heat
the unit in an over to about 100F, usually the
stuck goo will let go. The key is to release the
goo without damaging the optics.

The common solvent for the good seems to be acetone.
But, in an heated environment, it will be gone quickly.
So, you might have to try both approaches. Heat first
and try to release then introduce acetone. Once released,
put new oil on the threads. I have found that the best
oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000
Clock/Watchmaker 1045.

This little bottle will last a lifetime. I have no idea if
this is still available. If you cannot get it, I can send a
few drops of this to you and that should serve you well
into the future.

gary g.


At 09:31 AM 2/26/2009, you wrote:



} -----------------------------------------------------------------------
-----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


==============================Original
Headers==============================
11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009
11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n1R3giiv021379
11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009
21:42:45 -0600
11, 20 -- Message-Id: {200902270342.n1R3giiv021379-at-ns.microscopy.com}
11, 20 -- Received: (qmail 14792 invoked from network); 26 Feb 2009
19:46:27 -0800
11, 20 -- Received: by simscan 1.1.0 ppid: 14789, pid: 14790, t: 0.1064s
11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 20 -- by smtp1 with SMTP; 26 Feb 2009 19:46:27 -0800
11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 20 -- Date: Thu, 26 Feb 2009 19:42:34 -0800
11, 20 -- To: AJBowling-at-dow.com
11, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 20 -- Subject: Re: [Microscopy] Phase telescope
11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 20 -- In-Reply-To: {200902261731.n1QHVwG6013931-at-ns.microscopy.com}
11, 20 -- References: {200902261731.n1QHVwG6013931-at-ns.microscopy.com}
11, 20 -- Mime-Version: 1.0
11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 33 -- From AJBowling-at-dow.com Fri Feb 27 14:05:30 2009
20, 33 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.242.179])
20, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1RK5S2Q006669
20, 33 -- for {microscopy-at-microscopy.com} ; Fri, 27 Feb 2009 14:05:29 -0600
20, 33 -- X-VirusChecked: Checked
20, 33 -- X-Env-Sender: AJBowling-at-dow.com
20, 33 -- X-Msg-Ref: server-11.tower-86.messagelabs.com!1235765113!7036768!12
20, 33 -- X-StarScan-Version: 6.0.0; banners=-,-,-
20, 33 -- X-Originating-IP: [216.99.65.22]
20, 33 -- Received: (qmail 31310 invoked from network); 27 Feb 2009 20:05:26 -0000
20, 33 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22)
20, 33 -- by server-11.tower-86.messagelabs.com with RC4-SHA encrypted SMTP; 27 Feb 2009 20:05:26 -0000
20, 33 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830);
20, 33 -- Fri, 27 Feb 2009 15:05:25 -0500
20, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 33 -- Content-class: urn:content-classes:message
20, 33 -- MIME-Version: 1.0
20, 33 -- Content-Type: text/plain;
20, 33 -- charset="us-ascii"
20, 33 -- Subject: [Microscopy] Re: Phase telescope
20, 33 -- Date: Fri, 27 Feb 2009 15:05:24 -0500
20, 33 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1DA68F2-at-USMDLMDOWX032.dow.com}
20, 33 -- In-Reply-To: {200902270348.n1R3mcPD001894-at-ns.microscopy.com}
20, 33 -- X-MS-Has-Attach:
20, 33 -- X-MS-TNEF-Correlator:
20, 33 -- Thread-Topic: [Microscopy] Re: Phase telescope
20, 33 -- Thread-Index: AcmYjkhMpTymfnlBTUSLPw4w7yWfzgAaQIWg
20, 33 -- References: {200902270348.n1R3mcPD001894-at-ns.microscopy.com}
20, 33 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
20, 33 -- To: {microscopy-at-microscopy.com}
20, 33 -- X-OriginalArrivalTime: 27 Feb 2009 20:05:25.0052 (UTC) FILETIME=[BA4A1FC0:01C99916]
20, 33 -- Content-Transfer-Encoding: 8bit
20, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1RK5S2Q006669
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Fri, 27 Feb 2009 16:02:41 -0600
Subject: [Microscopy] Re: Phase telescope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You are, of course, correct. I tried 60C first and got nothing, but then
boosted the oven to around 100C and had success!

Good thing it's Friday!

andy b



________________________________

From: Straszheim, Warren E [M S E] [mailto:wesaia-at-iastate.edu]
Sent: Friday, February 27, 2009 3:36 PM
To: Bowling, Andrew (AJ)
Subject: RE: [Microscopy] Re: Phase telescope


What were those temperatures? You say you tried 60 degrees. That
must have been C, because 60F is below ambient around here, at least
when I have my furnace running.

60C = 140F and that is warmer than what Gary suggested.

However, if it works, that is the important thing. You may just
want to clarify it for the rest of us for future reference.

Warren S.

________________________________

From: AJBowling-at-dow.com
Sent: Fri 2/27/2009 2:07 PM
To: wesaia-at-iastate.edu
Subject: [Microscopy] Re: Phase telescope



------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html

------------------------------------------------------------------------
----

Ok, so I put the telescope in the oven at ~100F for an hour,
just as
Gary suggested, and this worked like a charm! I unscrewed it
enough to
expose some threads and I put a drop of Liquid Wrench with
Teflon on
them, worked it in a bit, and wiped off the liberated green goo
with a
kimwipe (I repeated this a few times). I tested it again after
putting
it in the fridge for a while to make sure that the grease
wouldn't set
when it cooled down, and it appears to be totally fixed. One
final note,
I had already tried to heat the telescope in the same oven set
at 60
degrees and it didn't work. Heating it higher was definitely
required.

Thanks!

Andy Bowling

-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Thursday, February 26, 2009 10:49 PM
To: Bowling, Andrew (AJ)
Subject: [Microscopy] Re: Phase telescope





------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society
of
America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html

------------------------------------------------------------------------
----

I've had this happen on old BH focusing telescopes.
The juice/oil...whatever solidifies over time.

This probably sounds ludicrous, but if you heat
the unit in an over to about 100F, usually the
stuck goo will let go. The key is to release the
goo without damaging the optics.

The common solvent for the good seems to be acetone.
But, in an heated environment, it will be gone quickly.
So, you might have to try both approaches. Heat first
and try to release then introduce acetone. Once released,
put new oil on the threads. I have found that the best
oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000
Clock/Watchmaker 1045.

This little bottle will last a lifetime. I have no idea if
this is still available. If you cannot get it, I can send a
few drops of this to you and that should serve you well
into the future.

gary g.


At 09:31 AM 2/26/2009, you wrote:




} -----------------------------------------------------------------------
-----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society
of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------
-----
}
} I have an Olympus centering telescope for a BH-2 phase contrast
} microscope that has frozen. Has anyone had any luck getting one
of
these
} un-stuck?
}
} Thanks,
}
} Andy Bowling
}
}
} ==============================Original
Headers==============================
} 4, 31 -- From AJBowling-at-dow.com Thu Feb 26 11:30:32 2009
} 4, 31 -- Received: from mail164.messagelabs.com
} (mail164.messagelabs.com [216.82.253.131])
} 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
with
} ESMTP id n1QHUViJ011530
} 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb
2009
} 11:30:31 -0600
} 4, 31 -- X-VirusChecked: Checked
} 4, 31 -- X-Env-Sender: AJBowling-at-dow.com
} 4, 31 -- X-Msg-Ref:
server-4.tower-164.messagelabs.com!1235669425!16997483!5
} 4, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,-
} 4, 31 -- X-Originating-IP: [204.136.184.21]
} 4, 31 -- Received: (qmail 12635 invoked from network); 26 Feb
2009
} 17:30:30 -0000
} 4, 31 -- Received: from mail3.dow.com (HELO
USFRPMDOWS001.dow.com)
} (204.136.184.21)
} 4, 31 -- by server-4.tower-164.messagelabs.com with RC4-SHA
} encrypted SMTP; 26 Feb 2009 17:30:30 -0000
} 4, 31 -- Received: from USMDLMDOWX032.dow.com
([163.198.215.63]) by
} USFRPMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830);
} 4, 31 -- Thu, 26 Feb 2009 11:30:27 -0600
} 4, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 4, 31 -- Content-class: urn:content-classes:message
} 4, 31 -- MIME-Version: 1.0
} 4, 31 -- Content-Type: text/plain;
} 4, 31 -- charset="us-ascii"
} 4, 31 -- Subject: Phase telescope
} 4, 31 -- Date: Thu, 26 Feb 2009 12:30:26 -0500
} 4, 31 -- Message-ID:
} {B72477374D7A74408DAC63801A0FDEA1DA6474-at-USMDLMDOWX032.dow.com}
} 4, 31 -- X-MS-Has-Attach:
} 4, 31 -- X-MS-TNEF-Correlator:
} 4, 31 -- Thread-Topic: Phase telescope
} 4, 31 -- Thread-Index: AcmYN+lCKx0Et6jfSY2nPb6qKQH1GA==
} 4, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
} 4, 31 -- To: {microscopy-at-microscopy.com}
} 4, 31 -- X-OriginalArrivalTime: 26 Feb 2009 17:30:27.0673 (UTC)

} FILETIME=[EA34EC90:01C99837]
} 4, 31 -- Content-Transfer-Encoding: 8bit
} 4, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by

} ns.microscopy.com id n1QHUViJ011530
} ==============================End of -
Headers==============================


==============================Original
Headers==============================
11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009
11, 20 -- Received: from smtp1.mc.surewest.net
(qsmtp.mc.surewest.net
[66.60.130.145])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)



From: gary-at-gaugler.com
Date: Fri, 27 Feb 2009 18:32:43 -0600
Subject: [Microscopy] Re: Re: Phase telescope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Whoa, I said 100F, not C. That would over 200F
Did you do F or C?

Like I said, the trick is to heat it up to loosen
the goo without damaging the optics. Usually 100F
works. If not, then about 125F. If that does not
work, then I put methanol around the periphery to
get it into the threads. Let it set for a day and
then do the oven at 100F. If that still does not
work, then I try to get acetone into the threads
and back to the over after a day. So far, along this
sequence, somewhere, the fix works.

I have not had to do this on phase units. But the
problem is still the same--hard goo that used to
be grease or oil.

gary g.


At 02:04 PM 2/27/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Fri Feb 27 18:32:43 2009
10, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1S0WeRR013235
10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Feb 2009 18:32:41 -0600
10, 20 -- Message-Id: {200902280032.n1S0WeRR013235-at-ns.microscopy.com}
10, 20 -- Received: (qmail 26885 invoked from network); 27 Feb 2009 16:36:30 -0800
10, 20 -- Received: by simscan 1.1.0 ppid: 26879, pid: 26881, t: 0.2924s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp1 with SMTP; 27 Feb 2009 16:36:30 -0800
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Fri, 27 Feb 2009 16:32:30 -0800
10, 20 -- To: AJBowling-at-dow.com
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] Re: Phase telescope
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200902272204.n1RM49kL028253-at-ns.microscopy.com}
10, 20 -- References: {200902272204.n1RM49kL028253-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jgschwen-at-rcn.com
Date: Sat, 28 Feb 2009 10:52:55 -0600
Subject: [Microscopy] Re: Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

The detector Ken referred to is the Compact Detector Unit (CDU) made
by EDAX.

http://www.edax.com/products/sku.cfm?ProductCAtegory_Id=4247&Product_Id=1009&SKU_Id=1037

As with all EDAX's modern SiLi detectors, it was designed to
automatically turn itself off when the LN2 was gone and the unit
started warming up. The CDU can also cool down and stabilize very
quickly, making it practical to leave at room temperature when not in
use.

Bottom line: things are going to vary according to detector design and
manufacturer. Your best bet is going to be contacting the company
which made the detector and ask them.

Jeff Gschwend


On Feb 25, 2009, at 1:21 PM, bozzola-at-siu.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This thread brought up a question.
}
} Suppose someone wants to mothball a LN2 chilled detector for a
} period of time.
}
} Is this feasible without significant damage?
}
} What precautions should be taken (bias off and voltage drained) prior
} to warming up, etc?
}
} What are the negative aspects of doing this (loss of resolution,
} presumably)?
}
} Thanks for the information.
} --
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} John J. Bozzola, Ph.D., Director
} Integrated Microscopy & Graphics Expertise (IMAGE)
} Southern Illinois University
} 750 Communications Drive - MC 4402
} Carbondale, IL 62901
} Telephone: 618-453-3730
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} ==============================Original
} Headers==============================
} 8, 19 -- From bozzola-at-siu.edu Wed Feb 25 13:19:55 2009
} 8, 19 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu
} [131.230.1.2])
} 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n1PJJtov024308
} 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:55
} -0600
} 8, 19 -- Received: from [131.230.177.136]
} (ws177136.microscope.siu.edu [131.230.177.136])
} 8, 19 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP
} id n1PJJrDS001332
} 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:54
} -0600 (CST)
} 8, 19 -- Mime-Version: 1.0
} 8, 19 -- Message-Id: {a06240802c5cb492177ae-at-[131.230.177.136]}
} 8, 19 -- In-Reply-To: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
} 8, 19 -- References: {200902251738.n1PHcCHW025499-at-ns.microscopy.com}
} 8, 19 -- Date: Wed, 25 Feb 2009 13:19:52 -0600
} 8, 19 -- To: Microscopy-at-microscopy.com
} 8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
} 8, 19 -- Subject: [Microscopy] Another EDX detector question
} 8, 19 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} 8, 19 -- X-Spam-Score: 0.00%
} 8, 19 -- X-MASF: 0.00%
} 8, 19 -- X-Whitelist: 0.00%
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
11, 24 -- From jgschwen-at-rcn.com Sat Feb 28 10:52:54 2009
11, 24 -- Received: from smtp02.lnh.mail.rcn.net (smtp02.lnh.mail.rcn.net [207.172.157.102])
11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1SGqs9D025986
11, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 28 Feb 2009 10:52:54 -0600
11, 24 -- Received: from mr02.lnh.mail.rcn.net ([207.172.157.22])
11, 24 -- by smtp02.lnh.mail.rcn.net with ESMTP; 28 Feb 2009 11:52:54 -0500
11, 24 -- Received: from smtp01.lnh.mail.rcn.net (smtp01.lnh.mail.rcn.net [207.172.4.11])
11, 24 -- by mr02.lnh.mail.rcn.net (MOS 3.10.4-GA)
11, 24 -- with ESMTP id POF69152;
11, 24 -- Sat, 28 Feb 2009 11:52:48 -0500 (EST)
11, 24 -- X-Auth-ID: jgschwen
11, 24 -- Received: from ip-69-33-68-33.chi.megapath.net ([69.33.68.33])
11, 24 -- by smtp01.lnh.mail.rcn.net with ESMTP; 28 Feb 2009 11:52:49 -0500
11, 24 -- Message-Id: {F77F6994-DDF7-405C-A7C5-47B15851FCD8-at-rcn.com}
11, 24 -- From: Jeff Gschwend {jgschwen-at-rcn.com}
11, 24 -- To: Microscopy-at-microscopy.com
11, 24 -- In-Reply-To: {200902251921.n1PJL6qm026803-at-ns.microscopy.com}
11, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 24 -- Content-Transfer-Encoding: 7bit
11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 24 -- Subject: Re: [Microscopy] Another EDX detector question
11, 24 -- Date: Sat, 28 Feb 2009 10:52:47 -0600
11, 24 -- References: {200902251921.n1PJL6qm026803-at-ns.microscopy.com}
11, 24 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: tiger3g3-at-yahoo.com
Date: Sat, 28 Feb 2009 20:23:13 -0600
Subject: [Microscopy] Merritt College Microscopy Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Re microscopy programs: we also offer a great, new certificate in
bioscience microscopy at Merritt College in Oakland. The focus (pun
intended) is on fluorescence, including confocal microscopy. It's a
one year program, night and weekends, and includes an internship and
a practicum. We have over 2 million dollars of scopes, all sorts of
systems and software, including two confocals, and a tissue culture
facility. Our instructors all have bio PhDs, including myself (and I
took the Woods Hole intensive in microscopy). We're a community
college so the cost is only $20/unit! We currently have 70 students
in the program and the first group is graduating and on the job
market. Check it out at: www.merritt.edu/microscopy

On a side note: we're thinking of setting up mini-courses for folks
already working in the field. Please let me know if there's interest
and what topics would be prefered!

We're also open to leasing time on our scopes, in return for taking
on a student as an intern (or just for a small fee instead)!

Also: graduation of the first group of students is Sat, March 28th,
6-10pm. All are welcome: it's a great chance to get to know the
program. The students will talk about their experiences, and
there'll be food and live music. It's in the student lounge, R
building of the campus: just come!

Last, but not least, if you need to hire a brilliant, motivated,
enthusiastic, hard-working, well-training microscopist, please let me
know! Our students are trained in both the theory and practice of
microscopy, and they have lots of hands-on experience. They're great
at ciritical thinking, troubleshooting, and juggling multiple
demands. Many had previous careers in computers, management,
graphics, photography, etc.

regards,
Gisele Giorgi, PhD
Director, Merritt Microscopy Program
Merritt College
12500 Campus Drive
Oakland, CA 94619
510-436-2618
ggiorgi-at-peralta.edu
www.merritt.edu/microscopy


At 7:56 AM -0600 11/24/08, zaluzec-at-microscopy.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
10, 28 -- From tiger3g3-at-yahoo.com Sat Feb 28 20:23:13 2009
10, 28 -- Received: from n56.bullet.mail.sp1.yahoo.com (n56.bullet.mail.sp1.yahoo.com [98.136.44.52])
10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n212NBIf021153
10, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Feb 2009 20:23:12 -0600
10, 28 -- Received: from [69.147.84.144] by n56.bullet.mail.sp1.yahoo.com with NNFMP; 01 Mar 2009 02:23:10 -0000
10, 28 -- Received: from [68.142.194.244] by t6.bullet.mail.sp1.yahoo.com with NNFMP; 01 Mar 2009 02:23:10 -0000
10, 28 -- Received: from [68.142.201.247] by t2.bullet.mud.yahoo.com with NNFMP; 01 Mar 2009 02:23:10 -0000
10, 28 -- Received: from [127.0.0.1] by omp408.mail.mud.yahoo.com with NNFMP; 01 Mar 2009 02:23:10 -0000
10, 28 -- X-Yahoo-Newman-Id: 138434.10361.bm-at-omp408.mail.mud.yahoo.com
10, 28 -- Received: (qmail 63860 invoked from network); 1 Mar 2009 02:23:09 -0000
10, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 28 -- s=s1024; d=yahoo.com;
10, 28 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:Mime-Version:X-Sender:Message-Id:In-Reply-To:References:Date:To:From:Subject:Content-Type;
10, 28 -- b=0Uk4KktfR4fFByhzsKIIMg34poaC5ElEK53eGE+5yfMG+4aqNPNxQWzxHRBT4pDvKES00A2PfDGlwAGL9eIAbKdXwIEVE+7OytB+boO/x9M40Uuk+9tjnVuQpgxfBLBQOuQ4OMgwTGr/Hq9Jtd5QmeAQ7c6MDfDVZ7c0wsqbUOw= ;
10, 28 -- Received: from unknown (HELO ?192.168.1.100?) (tiger3g3-at-98.207.93.115 with plain)
10, 28 -- by smtp123.plus.mail.sp1.yahoo.com with SMTP; 1 Mar 2009 02:23:09 -0000
10, 28 -- X-YMail-OSG: oGStBlAVM1nJFurMumsC317gTUEQuNHMypJVXtFqv_sv.4NkIpeT55OoUNMt8k7jFP73qqxBqw6YQjenyWCPHlvgoofGJxUDu9EQa3Fa4y68ERWbkhGlcPRfpk1yC_M_hRQibdYino_x.4cXzMcLOCIZGCvkxWCjI81SCnXxAK0bZg1rGDJYUzEmhBGKKh65qslfspkFVbiQo5Zngpk9N4Oa1xbusYVt_qgYb7dl5RcD.Gx8238KMnyCbE2biVi55a8U9pa0D7gAYriXxZ2xQWPAWGdJnw--
10, 28 -- X-Yahoo-Newman-Property: ymail-3
10, 28 -- Mime-Version: 1.0
10, 28 -- X-Sender: tiger3g3-at-pop.mail.yahoo.com
10, 28 -- Message-Id: {a05111b03c5cf9e1a9689-at-[192.168.1.100]}
10, 28 -- In-Reply-To: {200811241356.mAODuQRg027026-at-ns.microscopy.com}
10, 28 -- References: {200811241356.mAODuQRg027026-at-ns.microscopy.com}
10, 28 -- Date: Sat, 28 Feb 2009 18:23:08 -0800
10, 28 -- To: microscopy-at-microscopy.com
10, 28 -- From: Gisele Eliane Giorgi {tiger3g3-at-yahoo.com}
10, 28 -- Subject: [Microscopy] Merritt College Microscopy Program
10, 28 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Sat, 28 Feb 2009 22:21:41 -0600
Subject: [Microscopy] Another EDX detector question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You are right on.

The EDAX units automatically shut off if the detector
tip temperature is not at spec. This prompts a call
to their service folks. They are Johnny on the spot.

From my experience, letting EDAX or legacy Rontec (UHV) detectors
go dry for extended periods of time makes no difference.
However, the Rontec UHV units have wimpy Dewars. So, that is
the way that they are. But they work. They got bought up
by some other company...nothing new about this.

So you are right about contacting and getting credible responses
(problematic) from the company. For new procurements, a list of
specific requirements ought to be very helpful.

Disclaimer: No financial interest in EDAX/TSL or Ametek other
than being a very satisfied customer.

Dr. Gary Gaugler



At 08:54 AM 2/28/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
13, 20 -- From gary-at-gaugler.com Sat Feb 28 22:21:41 2009
13, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n214LeR3002139
13, 20 -- for {microscopy-at-microscopy.com} ; Sat, 28 Feb 2009 22:21:40 -0600
13, 20 -- Message-Id: {200903010421.n214LeR3002139-at-ns.microscopy.com}
13, 20 -- Received: (qmail 30220 invoked from network); 28 Feb 2009 20:25:43 -0800
13, 20 -- Received: by simscan 1.1.0 ppid: 30217, pid: 30218, t: 0.1475s
13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
13, 20 -- by smtp1 with SMTP; 28 Feb 2009 20:25:43 -0800
13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
13, 20 -- Date: Sat, 28 Feb 2009 20:21:37 -0800
13, 20 -- To: jgschwen-at-rcn.com
13, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
13, 20 -- Subject: Re: [Microscopy] Re: Another EDX detector question
13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
13, 20 -- In-Reply-To: {200902281654.n1SGsel2028385-at-ns.microscopy.com}
13, 20 -- References: {200902281654.n1SGsel2028385-at-ns.microscopy.com}
13, 20 -- Mime-Version: 1.0
13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Sun, 1 Mar 2009 11:22:19 -0600
Subject: [Microscopy] Tote bags

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For more years than I care to remember, I have been attending the M and
M annual meeting. Typically, I have come home each year with at least
one tote bag – gifts from the manufacturers. These bags have been
squirreled away (pack rat that I am) in a pile in a closet, causing me
to wonder why I continue to accept these gifts.

Recently during Spring cleaning looking at the pile of many bags, it
occurred to me that these bags are very similar to the bags that are now
on sale in every supermarket, to reduce greenhouse gases through not
using plastic bags. So I have started using M and M tote bags when I
go to the supermarket. They are great.

Am I the last to realize this? Has everyone else been doing this for ages?

--
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu

==============================Original Headers==============================
4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009
4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21HMJSC025229
4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 11:22:19 -0600
4, 21 -- Received: from [192.168.1.101] (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77])
4, 21 -- (authenticated bits=0)
4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n21HMGc0017150
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 12:22:18 -0500
4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu}
4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500
4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
4, 21 -- Reply-To: jae5-at-lehigh.edu
4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
4, 21 -- MIME-Version: 1.0
4, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
4, 21 -- Subject: Tote bags
4, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed
4, 21 -- Content-Transfer-Encoding: 8bit
4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu
4, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Sun, 1 Mar 2009 11:28:48 -0600
Subject: [Microscopy] Re: Tote bags

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is the sole reason that I collect them. I found that I had to
shorten the handles on the big white Zeiss bags, and due to their
size I have to instruct the bagger to put only the lightweight
items in them


On Sun Mar 01 12:23:12 EST 2009, jae5-at-lehigh.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} For more years than I care to remember, I have been attending the
} M and M annual meeting. Typically, I have come home each year
} with at least one tote bag ?? gifts from the manufacturers.
} These bags have been squirreled away (pack rat that I am) in a
} pile in a closet, causing me to wonder why I continue to accept
} these gifts.
}
} Recently during Spring cleaning looking at the pile of many bags,
} it occurred to me that these bags are very similar to the bags
} that are now on sale in every supermarket, to reduce greenhouse
} gases through not using plastic bags. So I have started using M
} and M tote bags when I go to the supermarket. They are great.
}
} Am I the last to realize this? Has everyone else been doing this
} for ages?
}
} -- Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
} ==============================Original
} Headers==============================
} 4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009
} 4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu
} [128.180.2.160])
} 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n21HMJSC025229
} 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009
} 11:22:19 -0600
} 4, 21 -- Received: from [192.168.1.101]
} (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77])
} 4, 21 -- (authenticated bits=0)
} 4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id
} n21HMGc0017150
} 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NOT)
} 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009
} 12:22:18 -0500
} 4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu}
} 4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500
} 4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
} 4, 21 -- Reply-To: jae5-at-lehigh.edu
} 4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
} 4, 21 -- MIME-Version: 1.0
} 4, 21 -- To: "MicroscopyListserver (E-mail)"
} {Microscopy-at-microscopy.com}
} 4, 21 -- Subject: Tote bags
} 4, 21 -- Content-Type: text/plain; charset=windows-1252;
} format=flowed
} 4, 21 -- Content-Transfer-Encoding: 8bit
} 4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter
} version 0.94.2 on rain.cc.lehigh.edu
} 4, 21 -- X-Virus-Status: Clean
} ==============================End of -
} Headers==============================
}
}



--
Greg Erdos
Assistant Director Emeritus
Micanopy FL


==============================Original Headers==============================
8, 22 -- From gwe-at-ufl.edu Sun Mar 1 11:28:47 2009
8, 22 -- Received: from smtp.ufl.edu (smtp03.osg.ufl.edu [128.227.74.70])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21HSlJP002465
8, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 11:28:47 -0600
8, 22 -- Received: from osgjas04.cns.ufl.edu (osgjas04.cns.ufl.edu [128.227.74.134])
8, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n21HSg8I032238;
8, 22 -- Sun, 1 Mar 2009 12:28:42 -0500
8, 22 -- Message-ID: {1464278855.260781235928522088.JavaMail.osg-at-osgjas04.cns.ufl.edu}
8, 22 -- Date: Sun, 1 Mar 2009 12:28:42 -0500 (EST)
8, 22 -- From: greg erdos {gwe-at-ufl.edu}
8, 22 -- Reply-To: gwe-at-ufl.edu
8, 22 -- To: jae5-at-lehigh.edu, Microscopy-at-microscopy.com
8, 22 -- Subject: Re: [Microscopy] Tote bags
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
8, 22 -- X-Originating-IP: 74.179.109.75 [74.179.109.75]
8, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-27_15:2009-02-26,2009-02-27,2009-03-01 signatures=0
8, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=6 spamscore=6 ipscore=0 phishscore=0 bulkscore=1 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903010110
8, 22 -- X-Spam-Level: *
8, 22 -- X-UFL-Spam-Level: *
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Sun, 1 Mar 2009 17:19:27 -0600
Subject: [Microscopy] TEM: Hot Stage Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

We have recently taken delivery of a new hot stage for our TEM. We are
interested in understanding the likely accuracy of the temperature read out.

In previous hot stage work, I have simply accepted the indicated
temperatures and put a 'reality factor' of +/-30deg C through it. This is a
reflection of poor thermal conductivity (for carbon support films at lower
temperatures {500degC), surface energy, residual stress, beam heating and
specimen preparation factors affecting the temperature at which phase
transformations, melting, recrystallisation etc occurs in the TEM.

However, we have some folk here who are interested in accurate temperature
work and so if anyone has developed or used readily available materials as
temperature calibration materials, I'd be interested in hearing from you.

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au






==============================Original Headers==============================
15, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 1 17:19:26 2009
15, 24 -- Received: from sanaa.ucc.usyd.edu.au (sanaa.ucc.usyd.edu.au [129.78.64.145])
15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21NJNFx001817
15, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 17:19:24 -0600
15, 24 -- Received: from sanaa.ucc.usyd.edu.au (localhost [127.0.0.1])
15, 24 -- by localhost (Postfix) with SMTP id 359F01775AA
15, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 10:19:21 +1100 (EST)
15, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19])
15, 24 -- by sanaa.ucc.usyd.edu.au (Postfix) with ESMTP id 28A131775A7
15, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 10:19:21 +1100 (EST)
15, 24 -- Received: from 172.17.185.136 ([172.17.185.136]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ;
15, 24 -- Sun, 1 Mar 2009 23:19:20 +0000
15, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618
15, 24 -- Date: Mon, 02 Mar 2009 10:19:20 +1100
15, 24 -- Subject: TEM: Hot Stage Calibration
15, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
15, 24 -- To: {Microscopy-at-microscopy.com}
15, 24 -- Message-ID: {C5D16328.34C%david.mitchell-at-emu.usyd.edu.au}
15, 24 -- Thread-Topic: TEM: Hot Stage Calibration
15, 24 -- Thread-Index: AcmaxCYVZJVpLAa3Ed6onQAjMrpsqg==
15, 24 -- Mime-version: 1.0
15, 24 -- Content-type: text/plain;
15, 24 -- charset="US-ASCII"
15, 24 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Sun, 1 Mar 2009 17:54:19 -0600
Subject: [Microscopy] Re: Tote bags

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Every year there is a local drive here in Santa Clara to donate
backpacks to students who cannot afford to purchase their own. I always
donate my MSA backpacks to students. It gets them a free backpack and
advertises Microscopy to everyone in the schools. I would hope that a
lot of MSA members would be able to make use of this way off finding a
second life for MSA backpacks as well.

John Mardinly,
Numonyx


-----Original Message-----
X-from: gwe-at-ufl.edu [mailto:gwe-at-ufl.edu]
Sent: Sunday, March 01, 2009 9:34 AM
To: MARDINLY, A

It is the sole reason that I collect them. I found that I had to
shorten the handles on the big white Zeiss bags, and due to their
size I have to instruct the bagger to put only the lightweight
items in them


On Sun Mar 01 12:23:12 EST 2009, jae5-at-lehigh.edu wrote:

}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} For more years than I care to remember, I have been attending the
} M and M annual meeting. Typically, I have come home each year
} with at least one tote bag ?? gifts from the manufacturers.
} These bags have been squirreled away (pack rat that I am) in a
} pile in a closet, causing me to wonder why I continue to accept
} these gifts.
}
} Recently during Spring cleaning looking at the pile of many bags,
} it occurred to me that these bags are very similar to the bags
} that are now on sale in every supermarket, to reduce greenhouse
} gases through not using plastic bags. So I have started using M
} and M tote bags when I go to the supermarket. They are great.
}
} Am I the last to realize this? Has everyone else been doing this
} for ages?
}
} -- Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
} ==============================Original
} Headers==============================
} 4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009
} 4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu
} [128.180.2.160])
} 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n21HMJSC025229
} 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009
} 11:22:19 -0600
} 4, 21 -- Received: from [192.168.1.101]
} (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77])
} 4, 21 -- (authenticated bits=0)
} 4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id
} n21HMGc0017150
} 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NOT)
} 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009
} 12:22:18 -0500
} 4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu}
} 4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500
} 4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
} 4, 21 -- Reply-To: jae5-at-lehigh.edu
} 4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
} 4, 21 -- MIME-Version: 1.0
} 4, 21 -- To: "MicroscopyListserver (E-mail)"
} {Microscopy-at-microscopy.com}
} 4, 21 -- Subject: Tote bags
} 4, 21 -- Content-Type: text/plain; charset=windows-1252;
} format=flowed
} 4, 21 -- Content-Transfer-Encoding: 8bit
} 4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter
} version 0.94.2 on rain.cc.lehigh.edu
} 4, 21 -- X-Virus-Status: Clean
} ==============================End of -
} Headers==============================
}
}



--
Greg Erdos
Assistant Director Emeritus
Micanopy FL


==============================Original
Headers==============================
8, 22 -- From gwe-at-ufl.edu Sun Mar 1 11:28:47 2009
8, 22 -- Received: from smtp.ufl.edu (smtp03.osg.ufl.edu
[128.227.74.70])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n21HSlJP002465
8, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009
11:28:47 -0600
8, 22 -- Received: from osgjas04.cns.ufl.edu (osgjas04.cns.ufl.edu
[128.227.74.134])
8, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id
n21HSg8I032238;
8, 22 -- Sun, 1 Mar 2009 12:28:42 -0500
8, 22 -- Message-ID:
{1464278855.260781235928522088.JavaMail.osg-at-osgjas04.cns.ufl.edu}
8, 22 -- Date: Sun, 1 Mar 2009 12:28:42 -0500 (EST)
8, 22 -- From: greg erdos {gwe-at-ufl.edu}
8, 22 -- Reply-To: gwe-at-ufl.edu
8, 22 -- To: jae5-at-lehigh.edu, Microscopy-at-microscopy.com
8, 22 -- Subject: Re: [Microscopy] Tote bags
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
8, 22 -- X-Originating-IP: 74.179.109.75 [74.179.109.75]
8, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure
engine=1.12.7400:2.4.4,1.2.40,4.0.166
definitions=2009-02-27_15:2009-02-26,2009-02-27,2009-03-01 signatures=0
8, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=6
spamscore=6 ipscore=0 phishscore=0 bulkscore=1 adultscore=0
classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000
definitions=main-0903010110
8, 22 -- X-Spam-Level: *
8, 22 -- X-UFL-Spam-Level: *
==============================End of -
Headers==============================



==============================Original Headers==============================
17, 29 -- From A.MARDINLY-at-numonyx.com Sun Mar 1 17:54:18 2009
17, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21NsIWP018767
17, 29 -- for {Microscopy-at-Microscopy.com} ; Sun, 1 Mar 2009 17:54:18 -0600
17, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23])
17, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id AD8812480F9;
17, 29 -- Sun, 1 Mar 2009 18:14:21 -0500 (EST)
17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
17, 29 -- Sun, 1 Mar 2009 18:54:15 -0500
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: RE: [Microscopy] Re: Tote bags
17, 29 -- Date: Sun, 1 Mar 2009 18:54:13 -0500
17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9E68C46-at-EXDRESBENMX012.numonyx.local}
17, 29 -- In-Reply-To: {200903011734.n21HY1vI019716-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: [Microscopy] Re: Tote bags
17, 29 -- Thread-Index: Acmak+vWQh3jKl3jSDiVJFsHz630IQANJi6Q
17, 29 -- References: {200903011734.n21HY1vI019716-at-ns.microscopy.com}
17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
17, 29 -- To: {gwe-at-ufl.edu}
17, 29 -- Cc: {Microscopy-at-Microscopy.com}
17, 29 -- X-OriginalArrivalTime: 01 Mar 2009 23:54:15.0254 (UTC) FILETIME=[06F42F60:01C99AC9]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n21NsIWP018767
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Mon, 2 Mar 2009 04:05:02 -0600
Subject: [Microscopy] ESEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello
We have a FEI XL30 ESEM-FEG dating back a few years now and are
beginning to encounter problems with sourcing spare parts. Just
wondering if anyone else out there is running an xl30 esem and if so are
you having similar problems now they are no longer made,or does everyone
have a Quanta these days?

Thanks
Nikki Weston
School M3
University of Nottingham

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Mon Mar 2 04:05:01 2009
5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5])
5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22A4ve7017438
5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 04:04:58 -0600
5, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11])
5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 32 -- id 1Le4yO-0007mK-P5
5, 32 -- for microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:02:08 +0000
5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 32 -- Mon, 2 Mar 2009 10:01:48 +0000
5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
5, 32 -- Content-class: urn:content-classes:message
5, 32 -- MIME-Version: 1.0
5, 32 -- Content-Type: text/plain;
5, 32 -- charset="us-ascii"
5, 32 -- Subject: ESEM users
5, 32 -- Date: Mon, 2 Mar 2009 10:01:48 -0000
5, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94EF23C7-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 32 -- X-MS-Has-Attach:
5, 32 -- X-MS-TNEF-Correlator:
5, 32 -- Thread-Topic: ESEM users
5, 32 -- Thread-Index: AcmbHeakr1F3obFISgOwE1S60jpp0A==
5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 32 -- To: {microscopy-at-microscopy.com}
5, 32 -- X-OriginalArrivalTime: 02 Mar 2009 10:01:48.0940 (UTC) FILETIME=[E70B3CC0:01C99B1D]
5, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
5, 32 -- X-UoN-MailScanner: Found to be clean
5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 32 -- X-Spam-Status: No
5, 32 -- Content-Transfer-Encoding: 8bit
5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22A4ve7017438
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Mon, 2 Mar 2009 04:59:46 -0600
Subject: [Microscopy] ESEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nikki, no problems with our tungsten gun XL30 ESEM re support so far.

On a related theme we were worrying about our Philips CM10 TEM which
ceases to be supported, I believe, in 2011. Unofficially should be able
to keep them going for a long time as there is a large customer base in
the UK.

Dave

-----Original Message-----
X-from: Nicola.Weston-at-nottingham.ac.uk
[mailto:Nicola.Weston-at-nottingham.ac.uk]
Sent: 02 March 2009 10:19
To: David Patton

Hello
We have a FEI XL30 ESEM-FEG dating back a few years now and are
beginning to encounter problems with sourcing spare parts. Just
wondering if anyone else out there is running an xl30 esem and if so are
you having similar problems now they are no longer made,or does everyone
have a Quanta these days?

Thanks
Nikki Weston
School M3
University of Nottingham

This message has been checked for viruses but the contents of an
attachment
may still contain software viruses, which could damage your computer
system:
you are advised to perform your own checks. Email communications with
the
University of Nottingham may be monitored as permitted by UK
legislation.



==============================Original
Headers==============================
5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Mon Mar 2 04:05:01 2009
5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk
[128.243.44.5])
5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n22A4ve7017438
5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009
04:04:58 -0600
5, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11])
5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 32 -- id 1Le4yO-0007mK-P5
5, 32 -- for microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:02:08
+0000
5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231])
by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 32 -- Mon, 2 Mar 2009 10:01:48 +0000
5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
5, 32 -- Content-class: urn:content-classes:message
5, 32 -- MIME-Version: 1.0
5, 32 -- Content-Type: text/plain;
5, 32 -- charset="us-ascii"
5, 32 -- Subject: ESEM users
5, 32 -- Date: Mon, 2 Mar 2009 10:01:48 -0000
5, 32 -- Message-ID:
{1E0CCC81FDE82D4C8DDE1A8F44080D94EF23C7-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 32 -- X-MS-Has-Attach:
5, 32 -- X-MS-TNEF-Correlator:
5, 32 -- Thread-Topic: ESEM users
5, 32 -- Thread-Index: AcmbHeakr1F3obFISgOwE1S60jpp0A==
5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 32 -- To: {microscopy-at-microscopy.com}
5, 32 -- X-OriginalArrivalTime: 02 Mar 2009 10:01:48.0940 (UTC)
FILETIME=[E70B3CC0:01C99B1D]
5, 32 -- X-UoN-MailScanner-Information: Please contact
staff-it-helpline-at-nottingham.ac.uk for more information
5, 32 -- X-UoN-MailScanner: Found to be clean
5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 32 -- X-Spam-Status: No
5, 32 -- Content-Transfer-Encoding: 8bit
5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n22A4ve7017438
==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
18, 34 -- From David.Patton-at-uwe.ac.uk Mon Mar 2 04:59:45 2009
18, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65])
18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n22Axiin032408
18, 34 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 04:59:45 -0600
18, 34 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp
18, 34 -- id 2346_3c61125a_0719_11de_b5bc_00142221cca9;
18, 34 -- Mon, 02 Mar 2009 10:59:43 +0000
18, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
18, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
18, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
18, 34 -- 2005)) with SMTP id {0KFV00AA2L7JLD-at-mta02.uwe.ac.uk} for
18, 34 -- microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:59:43 +0000 (GMT)
18, 34 -- Date: Mon, 02 Mar 2009 10:58:41 +0000
18, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk}
18, 34 -- Subject: RE: [Microscopy] ESEM users & TEMs after official support ends!
18, 34 -- In-reply-to: {200903021019.n22AJFVq023443-at-ns.microscopy.com}
18, 34 -- To: microscopy-at-microscopy.com
18, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB046778D5-at-egen-uwe01}
18, 34 -- MIME-version: 1.0
18, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
18, 34 -- Content-type: text/plain; charset=us-ascii
18, 34 -- Content-class: urn:content-classes:message
18, 34 -- Thread-topic: [Microscopy] ESEM users & TEMs after official support ends!
18, 34 -- Thread-index: AcmbIF09/sklQly5RVyM8jz7jRHMZAABK9IQ
18, 34 -- X-MS-Has-Attach:
18, 34 -- X-MS-TNEF-Correlator:
18, 34 -- References: {200903021019.n22AJFVq023443-at-ns.microscopy.com}
18, 34 -- X-NAIMIME-Disclaimer: 1
18, 34 -- X-NAIMIME-Modified: 1
18, 34 -- X-NAI-Spam-Score: 0
18, 34 -- X-NAI-Spam-Rules: 1 Rules triggered
18, 34 -- RV3221=0
18, 34 -- Content-Transfer-Encoding: 8bit
18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22Axiin032408
==============================End of - Headers==============================




From: y.han-at-sheffield.ac.uk
Date: Mon, 2 Mar 2009 08:16:11 -0600
Subject: [Microscopy] viaWWW: Sample drifting in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: y.han-at-sheffield.ac.uk
Name: Yisong Han

Organization: University of Sheffield

Title-Subject: [Filtered] Sample drifting in TEM

Question: Dear All,

I had a chance to look at a bulk ceramic which contains Pb, Mg and W.
I knew it has a phase transition temperature at around 40 degree C. I
indeed saw domain movement with the electron beam and I guess the
phase transition occurred due to a heating effect by the beam and I
was not surprised about this.

But I still noticed that the shadow of the objective aperture moved
around a lot while I was moving the sample. Also the diffraction
spots were highly distorted from what we normally see and this could
not be corrected by a combined adjustment of C2 and diffraction
focus. I know this sample should be cooled or heated to avoid such a
transition taking place. I was wondering if anybody has encountered
such a situation and can give an explanation about the drifting of
the objective aperture and distortion of diffraction spots. Is this
material is slightly magnetic or something else... Thanks very much
for your attention.

Yisong

Login Host: 143.167.204.40
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Mar 2 08:16:10 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22EG9L6021109
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 08:16:10 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240803c5d19a9525ba-at-[206.69.208.22]}
9, 11 -- Date: Mon, 2 Mar 2009 08:16:04 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Sample drifting in TEM
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 2 Mar 2009 08:31:00 -0600
Subject: [Microscopy] Re: Tote bags

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yep.
Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
3, 27 -- From oshel1pe-at-cmich.edu Mon Mar 2 08:31:00 2009
3, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22EUxhO032705
3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 08:30:59 -0600
3, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
3, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n22EUsnA022194
3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 09:30:55 -0500
3, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
3, 27 -- Mon, 2 Mar 2009 09:30:38 -0500
3, 27 -- Mime-Version: 1.0
3, 27 -- Message-Id: {f06240803c5d19dbdc491-at-[141.209.160.249]}
3, 27 -- In-Reply-To: {200903011726.n21HQwRW031799-at-ns.microscopy.com}
3, 27 -- References: {200903011726.n21HQwRW031799-at-ns.microscopy.com}
3, 27 -- Date: Mon, 2 Mar 2009 09:30:36 -0500
3, 27 -- To: Microscopy-at-microscopy.com
3, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 27 -- Subject: Re: [Microscopy] Tote bags
3, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
3, 27 -- X-OriginalArrivalTime: 02 Mar 2009 14:30:38.0532 (UTC) FILETIME=[7507A840:01C99B43]
3, 27 -- X-Canit-CHI2: 0.00
3, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 27 -- X-Spam-Score: -2.60 () [Hold at 5.00] L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
3, 27 -- X-CanItPRO-Stream: default
3, 27 -- X-Canit-Stats-ID: 9685874 - 6d29878c9cbe
3, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
3, 27 -- Content-Transfer-Encoding: 8bit
3, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22EUxhO032705
==============================End of - Headers==============================




From: contact-at-integrityscientific.com
Date: Mon, 2 Mar 2009 09:06:00 -0600
Subject: [Microscopy] Re: viaWWW: Sample drifting in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Yisong,
is your sample ferroelectric? I think you have problems with sample
charging; I have seen similar things myself recently. The interaction
of charge on the sample and the spontaneous ferroelectric charge in the
material can certainly cause domain walls to move. I have found that
plasma cleaning of ceramic TEM samples may get rid of contamination, but
can make them impossible to work with because of this. If you didn't
plasma clean your specimen, try a light carbon coat on both sides of the
specimen to reduce charging and try to keep the beam current density low.

Also, given the level of complexity that can occur in some oxides your
'distorted spots' may actually be showing real things, such as
modulations in composition ('tweed' structure, defects, ordered oxygen
vacancies, etc. etc. etc. etc.)

Good luck

Richard Beanland

y.han-at-sheffield.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: y.han-at-sheffield.ac.uk
} Name: Yisong Han
}
} Organization: University of Sheffield
}
} Title-Subject: [Filtered] Sample drifting in TEM
}
} Question: Dear All,
}
} I had a chance to look at a bulk ceramic which contains Pb, Mg and W.
} I knew it has a phase transition temperature at around 40 degree C. I
} indeed saw domain movement with the electron beam and I guess the
} phase transition occurred due to a heating effect by the beam and I
} was not surprised about this.
}
} But I still noticed that the shadow of the objective aperture moved
} around a lot while I was moving the sample. Also the diffraction
} spots were highly distorted from what we normally see and this could
} not be corrected by a combined adjustment of C2 and diffraction
} focus. I know this sample should be cooled or heated to avoid such a
} transition taking place. I was wondering if anybody has encountered
} such a situation and can give an explanation about the drifting of
} the objective aperture and distortion of diffraction spots. Is this
} material is slightly magnetic or something else... Thanks very much
} for your attention.
}
} Yisong
}
} Login Host: 143.167.204.40
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 11 -- From zaluzec-at-microscopy.com Mon Mar 2 08:16:10 2009
} 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22EG9L6021109
} 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 08:16:10 -0600
} 9, 11 -- Mime-Version: 1.0
} 9, 11 -- Message-Id: {p06240803c5d19a9525ba-at-[206.69.208.22]}
} 9, 11 -- Date: Mon, 2 Mar 2009 08:16:04 -0600
} 9, 11 -- To: microscopy-at-microscopy.com
} 9, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver)
} 9, 11 -- Subject: viaWWW: Sample drifting in TEM
} 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}


==============================Original Headers==============================
6, 29 -- From contact-at-integrityscientific.com Mon Mar 2 09:06:00 2009
6, 29 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8])
6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22F5wwt017408
6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 09:05:59 -0600
6, 29 -- Received: from localhost (localhost [127.0.0.1])
6, 29 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n22F5vAV007516;
6, 29 -- Mon, 2 Mar 2009 15:05:57 GMT
6, 29 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk
6, 29 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1])
6, 29 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024)
6, 29 -- with LMTP id LxcXA9wBo4SV; Mon, 2 Mar 2009 15:05:52 +0000 (GMT)
6, 29 -- Received: from [137.205.164.171] (hosts-137-205-164-171 [137.205.164.171])
6, 29 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n22F5NCU007318;
6, 29 -- Mon, 2 Mar 2009 15:05:23 GMT
6, 29 -- X-Envelope-From: contact-at-integrityscientific.com
6, 29 -- Message-ID: {49ABF59B.3060704-at-integrityscientific.com}
6, 29 -- Date: Mon, 02 Mar 2009 15:04:59 +0000
6, 29 -- From: Richard Beanland {contact-at-integrityscientific.com}
6, 29 -- Reply-To: contact-at-integrityscientific.com
6, 29 -- Organization: Integrity Scientific Ltd
6, 29 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 29 -- MIME-Version: 1.0
6, 29 -- To: y.han-at-sheffield.ac.uk, microscopy-at-microscopy.com
6, 29 -- Subject: Re: [Microscopy] viaWWW: Sample drifting in TEM
6, 29 -- References: {200903021429.n22ETQ5w031000-at-ns.microscopy.com}
6, 29 -- In-Reply-To: {200903021429.n22ETQ5w031000-at-ns.microscopy.com}
6, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 29 -- Content-Transfer-Encoding: 7bit
6, 29 -- X-DCC-Warwick-Metrics: nightshade; whitelist
==============================End of - Headers==============================




From: dking-at-a123systems.com
Date: Mon, 2 Mar 2009 11:20:59 -0600
Subject: [Microscopy] Os Staining to Contrast Polymer in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am uncertain what you mean by "ceases to be supported".

My understanding is that there are several possible meanings:
1. The supplier will not offer service contracts or repairs.
2. The supplier will be disposing of all specific spares but still do
contracts and repairs eg if you have parts.
3. The supplier will no longer be re-stocking specific parts but will
hold a diminishing stock and do service repairs.

Obviously if statement 2 or 3 is true then the machine could still be
serviced by the supplier providing you or they have the parts. So it
is useful to know which is true.

I don't know if this helps but it may give you an extra year or two.

Malcolm

Malcolm Haswell
e.m. unit
The Faculty of Applied Sciences
University of Sunderland
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: David.Patton-at-uwe.ac.uk

Dear All, does anyone have or know where I can find procedures for Osmium staining of polymeric materials?

Thanks in advance for your help.

Danny King
Test Chemist
A123 Systems
3850 Research Park Dr.
Ann Arbor, MI 48108



==============================Original Headers==============================
4, 26 -- From dking-at-a123systems.com Mon Mar 2 11:20:59 2009
4, 26 -- Received: from us-bos-mail2.a123systems.com (mail.a123systems.com [208.58.17.130])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22HKx1m017509
4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 11:20:59 -0600
4, 26 -- Received: from US-BOS-MAIL1.a123systems.com ([172.16.10.226]) by us-bos-mail2.a123systems.com with Microsoft SMTPSVC(6.0.3790.3959);
4, 26 -- Mon, 2 Mar 2009 12:19:07 -0500
4, 26 -- Received: from US-BOS-MAIL1.a123systems.com ([::1]) by
4, 26 -- US-BOS-MAIL1.a123systems.com ([fe80::5efe:172.16.10.226%12]) with mapi; Mon,
4, 26 -- 2 Mar 2009 12:19:07 -0500
4, 26 -- From: Danny King {dking-at-a123systems.com}
4, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
4, 26 -- Date: Mon, 2 Mar 2009 12:19:06 -0500
4, 26 -- Subject: Os Staining to Contrast Polymer in SEM
4, 26 -- Thread-Topic: Os Staining to Contrast Polymer in SEM
4, 26 -- Thread-Index: AcmbWv3yZAG4nHhzSByexVmpD786mw==
4, 26 -- Message-ID: {9D7A68726BF97948A16CD4BF00DD249D02D51A94FD-at-US-BOS-MAIL1.a123systems.com}
4, 26 -- Accept-Language: en-US
4, 26 -- Content-Language: en-US
4, 26 -- X-MS-Has-Attach:
4, 26 -- X-MS-TNEF-Correlator:
4, 26 -- acceptlanguage: en-US
4, 26 -- Content-Type: text/plain; charset="us-ascii"
4, 26 -- MIME-Version: 1.0
4, 26 -- X-OriginalArrivalTime: 02 Mar 2009 17:19:07.0588 (UTC) FILETIME=[FE7F3040:01C99B5A]
4, 26 -- Content-Transfer-Encoding: 8bit
4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22HKx1m017509
==============================End of - Headers==============================




From: lherault-at-bu.edu
Date: Mon, 2 Mar 2009 11:29:02 -0600
Subject: [Microscopy] ESEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We're still running an XL 20, fortunately always under service contract. We
have not had a problem with parts yet.

Ron L'Herault
B.U. School of Dental Medicine,
Biomaterials Division.

-----Original Message-----
X-from: Nicola.Weston-at-nottingham.ac.uk [mailto:Nicola.Weston-at-nottingham.ac.uk]

Sent: Monday, March 02, 2009 5:16 AM
To: lherault-at-bu.edu

Hello
We have a FEI XL30 ESEM-FEG dating back a few years now and are
beginning to encounter problems with sourcing spare parts. Just
wondering if anyone else out there is running an xl30 esem and if so are
you having similar problems now they are no longer made,or does everyone
have a Quanta these days?

Thanks
Nikki Weston


==============================Original Headers==============================
11, 22 -- From lherault-at-bu.edu Mon Mar 2 11:29:02 2009
11, 22 -- Received: from vms173001pub.verizon.net (vms173001pub.verizon.net [206.46.173.1])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22HT2pN030854
11, 22 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 11:29:02 -0600
11, 22 -- Received: from ronlherault ([71.162.90.228]) by vms173001.mailsrvcs.net
11, 22 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
11, 22 -- with ESMTPA id {0KFW00GNX385N3HZ-at-vms173001.mailsrvcs.net} for
11, 22 -- microscopy-at-microscopy.com; Mon, 02 Mar 2009 11:28:55 -0600 (CST)
11, 22 -- Reply-to: {lherault-at-bu.edu}
11, 22 -- From: "Ron L'Herault" {lherault-at-bu.edu}
11, 22 -- To: {microscopy-at-microscopy.com}
11, 22 -- References: {200903021016.n22AGCEg022238-at-ns.microscopy.com}
11, 22 -- Subject: RE: [Microscopy] ESEM users
11, 22 -- Date: Mon, 02 Mar 2009 12:28:51 -0500
11, 22 -- Message-id: {884189E80C084796A9A0727B16C15197-at-ronlherault}
11, 22 -- MIME-version: 1.0
11, 22 -- Content-type: text/plain; charset=US-ASCII
11, 22 -- Content-transfer-encoding: 7bit
11, 22 -- X-Mailer: Microsoft Office Outlook 11
11, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
11, 22 -- In-reply-to: {200903021016.n22AGCEg022238-at-ns.microscopy.com}
11, 22 -- Thread-index: AcmbH+2jqeAsRfXoS5ydfaBGEa4W5wAPCVew
==============================End of - Headers==============================




From: rbeavers-at-mail.smu.edu
Date: Mon, 2 Mar 2009 12:01:40 -0600
Subject: [Microscopy] clay separations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Markus

yes, in fact if you keep in close contact with companies they may even
let you know if they are having a "closing down/stock clearance" sale
at which point it may be worth stocking up.

Malcolm

----- Original Message -----
X-from: "Markus F. Meyenhofer" {micro-at-superlink.net}

Not sure if this group can offer any help but thought I would try.

I am trying to locate information on a Beckman Continuous Particle Electrophoresis System for clay separations mentioned in "The American Mineralogist, VOL 54, MAY-JUNE, 1969" paper by James I. Drever at Princeton.

System is no longer made, but hoping someone may have seen one or have one in a storeroom somewhere. Would love to find a system or locate a manual or detail drawing of the electrophoresis cell.

Any help is much appreciated.

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
7, 26 -- From rbeavers-at-mail.smu.edu Mon Mar 2 12:01:39 2009
7, 26 -- Received: from sxet1p2.systems.smu.edu (sxet1p2.systems.smu.edu [129.119.65.147])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22I1dG9027491
7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 12:01:39 -0600
7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by
7, 26 -- sxet1p2.systems.smu.edu (129.119.65.147) with Microsoft SMTP Server (TLS) id
7, 26 -- 8.1.340.0; Mon, 2 Mar 2009 12:01:39 -0600
7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 2 Mar 2009
7, 26 -- 12:01:32 -0600
7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 26 -- Date: Mon, 2 Mar 2009 12:01:30 -0600
7, 26 -- Subject: clay separations
7, 26 -- Thread-Topic: clay separations
7, 26 -- Thread-Index: AcmbYOo79ac06aNhRcuAmXY9k/TMLw==
7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB20C1FDA497-at-SXMBXC.systems.smu.edu}
7, 26 -- Accept-Language: en-US
7, 26 -- Content-Language: en-US
7, 26 -- X-MS-Has-Attach:
7, 26 -- X-MS-TNEF-Correlator:
7, 26 -- acceptlanguage: en-US
7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
7, 26 -- MIME-Version: 1.0
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22I1dG9027491
==============================End of - Headers==============================




From: r-holdford-at-ti.com
Date: Mon, 2 Mar 2009 18:10:08 -0600
Subject: [Microscopy] Texas Society for Microscopy Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Texas Society for Microscopy Spring 2009 Meeting
April 23-25, 2009 at the

Marriott Solana Hotel, Westlake, TX
(preferred room rate cut-off date: April 3, 2009)


Friday Guest Speakers
"High Resolution Fluorescence Measurements of the Muscle Contractile
Apparatus"
Doug Root, Associate Professor, University of North Texas

"A Demonstration of 3D Tomography"
Lee Pullan, Senior Applications Engineer, FEI Company, Portland, Oregon

"Polymer Microscopy"
Gary M. Brown, Exxon Mobil, Baytown, Texas

"Introduction to EDS and its Application in the Research in Pierce's
disease of Grapes and Citrus Chlorosis"
Brebo Leite, ThermoFisher

All forms and hotel information available on our web site:
http://www.texasmicroscopy.org/ under the Spring 2009 nav button.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA
Texas Instruments, Inc.
13536 N. Central Expressway MS 940
Dallas, TX 75243
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
10, 20 -- From r-holdford-at-ti.com Mon Mar 2 18:10:06 2009
10, 20 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n230A42R020211
10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 18:10:06 -0600
10, 20 -- Received: from dlep36.itg.ti.com ([157.170.170.91])
10, 20 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id n2309wTu002932
10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 18:10:03 -0600
10, 20 -- Received: from [156.117.248.174] (localhost [127.0.0.1])
10, 20 -- by dlep36.itg.ti.com (8.13.8/8.13.8) with ESMTP id n2309voj021617
10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 18:09:58 -0600 (CST)
10, 20 -- Message-ID: {49AC7555.7010409-at-ti.com}
10, 20 -- Date: Mon, 02 Mar 2009 18:09:57 -0600
10, 20 -- From: Becky Holdford {r-holdford-at-ti.com}
10, 20 -- Organization: SC Packaging Development -- FA Development
10, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
10, 20 -- MIME-Version: 1.0
10, 20 -- To: MSA Listserver {Microscopy-at-microscopy.com}
10, 20 -- Subject: Texas Society for Microscopy Spring Meeting
10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Tue, 3 Mar 2009 04:40:09 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
I think I should clarify that we have a service contract & good support
from FEI. My main concern is the integrated DX4 pc, which has been
upgraded as far as possible. So far we have been able to buy parts from
FEI but I am worried about future sourcing of spares for our system as
parts are no longer made.
It's good to know about second hand supplies from elsewhere though,
thanks for all your replies.

Regards
Nikki







Nikki

Are you saying that FEI will not sell you the parts?

regards,

Jim


} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2
Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From:
Nicola.Weston-at-nottingham.ac.uk } Reply-to:
Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver"
{microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users }
Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Hello
} We have a FEI XL30 ESEM-FEG dating back a few years now and are }
beginning to encounter problems with sourcing spare parts. Just }
wondering if anyone else out there is running an xl30 esem and if so are
} you having similar problems now they are no longer made,or does
everyone } have a Quanta these days?
}
} Thanks
} Nikki Weston
} School M3
} University of Nottingham

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009
18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ae7QU031210
18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 04:40:07 -0600
18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
18, 32 -- id 1LeS1J-0007KK-Tf
18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42 +0000
18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000
18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
18, 32 -- Content-class: urn:content-classes:message
18, 32 -- MIME-Version: 1.0
18, 32 -- Content-Type: text/plain;
18, 32 -- charset="us-ascii"
18, 32 -- Subject: Esem users
18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000
18, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk}
18, 32 -- X-MS-Has-Attach:
18, 32 -- X-MS-TNEF-Correlator:
18, 32 -- Thread-Topic: Esem users
18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw==
18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
18, 32 -- To: {microscopy-at-microscopy.com}
18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC) FILETIME=[37B53760:01C99BEC]
18, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
18, 32 -- X-UoN-MailScanner: Found to be clean
18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
18, 32 -- X-Spam-Status: No
18, 32 -- Content-Transfer-Encoding: 8bit
18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ae7QU031210
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Tue, 3 Mar 2009 06:47:00 -0600
Subject: [Microscopy] Ultramicrotomy - diamond knife question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Maybe a dumb question but can a cryo diamond knife be used to cut resin
sections at room temperature?

TIA
Nikki

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 06:46:59 2009
5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5])
5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ckw5M017434
5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 06:46:59 -0600
5, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 32 -- id 1LeU1F-0000iJ-Vi
5, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 12:46:46 +0000
5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 32 -- Tue, 3 Mar 2009 12:46:40 +0000
5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
5, 32 -- Content-class: urn:content-classes:message
5, 32 -- MIME-Version: 1.0
5, 32 -- Content-Type: text/plain;
5, 32 -- charset="us-ascii"
5, 32 -- Subject: Ultramicrotomy - diamond knife question
5, 32 -- Date: Tue, 3 Mar 2009 12:46:40 -0000
5, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D81A-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 32 -- X-MS-Has-Attach:
5, 32 -- X-MS-TNEF-Correlator:
5, 32 -- Thread-Topic: Ultramicrotomy - diamond knife question
5, 32 -- Thread-Index: Acmb/hkKpFV7u4onQ1WNLoqKQKQAbg==
5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 32 -- To: {microscopy-at-microscopy.com}
5, 32 -- X-OriginalArrivalTime: 03 Mar 2009 12:46:40.0853 (UTC) FILETIME=[197F2050:01C99BFE]
5, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
5, 32 -- X-UoN-MailScanner: Found to be clean
5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 32 -- X-Spam-Status: No
5, 32 -- Content-Transfer-Encoding: 8bit
5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ckw5M017434
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Tue, 3 Mar 2009 07:57:25 -0600
Subject: [Microscopy] Re: Ultramicrotomy - diamond knife question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi Nikki,

It really depends upon which type of cryo knife you have. In the case
of ours all the cryo knives would cut the resin, but you would need
the 'wet' knife to keep the section wet.

JD Arnott

Disclaimer: Ladd research sells diamond knives

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com


At 07:58 AM 3/3/2009, Nicola.Weston-at-nottingham.ac.uk wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America






==============================Original Headers==============================
19, 27 -- From jd-at-laddresearch.com Tue Mar 3 07:57:25 2009
19, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29])
19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23DvOa3001134
19, 27 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 07:57:24 -0600
19, 27 -- Received: from 1LeV7Z-0000RK-Th by bean.electric.net with emc1-ok (Exim 4.69)
19, 27 -- (envelope-from {jd-at-laddresearch.com} )
19, 27 -- id 1LeV7Z-0000Rq-Uz; Tue, 03 Mar 2009 05:57:21 -0800
19, 27 -- Received: by emcmailer; Tue, 03 Mar 2009 05:57:21 -0800
19, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
19, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256)
19, 27 -- (Exim 4.69)
19, 27 -- (envelope-from {jd-at-laddresearch.com} )
19, 27 -- id 1LeV7Z-0000RK-Th; Tue, 03 Mar 2009 05:57:21 -0800
19, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
19, 27 -- Date: Tue, 03 Mar 2009 08:57:17 -0500
19, 27 -- To: Nicola.Weston-at-nottingham.ac.uk
19, 27 -- From: jd {jd-at-laddresearch.com}
19, 27 -- Subject: Re: [Microscopy] Ultramicrotomy - diamond knife question
19, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
19, 27 -- In-Reply-To: {200903031258.n23CwtSH026420-at-ns.microscopy.com}
19, 27 -- References: {200903031258.n23CwtSH026420-at-ns.microscopy.com}
19, 27 -- Mime-Version: 1.0
19, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
19, 27 -- X-Outbound-IP: 216.204.198.170
19, 27 -- X-Env-From: jd-at-laddresearch.com
19, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
19, 27 -- Message-Id: {E1LeV7Z-0000Rq-Uz-at-bean.electric.net}
==============================End of - Headers==============================




From: spb-at-mwrn.com
Date: Tue, 3 Mar 2009 08:18:03 -0600
Subject: [Microscopy] viaWWW: Workshop on Immunogold Silver Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both spb-at-mwrn.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: spb-at-mwrn.com
Name: Susanne Brandom

Organization: MC Services

Title-Subject: [Filtered] Workshop on Immunogold Silver Staining

Question: The Aurion ImmunoGold Silver Staining Workshop

Aurion and Electron Microscopy Sciences will hold a workshop on
Immunogold Silver Staining at Albert Einstein College of Medicine
from May 18 to 21. The course will be taught by Mr. Peter van de Plas
who has been with Aurion since 1991. He worked closely together with
Dr. Leunissen in founding a firm basis for Aurion and contributed to
the development of product. He has been invited to many international
microscopy conferences and workshops and is especially experienced in
providing hands-on training.

The course will cover:

The properties of gold particles and their protein conjugates
Theories underlying immunogold labeling protocols.
Silver enhancement of gold particles.
Immunogold labeling on a variety of sample preparations for LM.
Immunogold labeling for EM
Pre-/post-embedding double immunogold labeling.
Background minimization in immunogold labeling

Participants are encouraged to bring their own samples, that will be
processed under expert guidance. For more information contact Stacie
Kirsch at Electron Microscopy Sciences. Please email
stacie-at-ems-secure.com or call 215-412-8402 for details.



Login Host: 66.189.46.25
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Tue Mar 3 08:18:03 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23EI2bp017564
12, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 08:18:03 -0600
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240802c5d2ec7b5930-at-[206.69.208.22]}
12, 11 -- Date: Tue, 3 Mar 2009 08:17:59 -0600
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: spb-at-mwrn.com (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Workshop on Immunogold Silver Staining
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 3 Mar 2009 09:12:29 -0600
Subject: [Microscopy] viaWWW: Epon/PAS stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Annie-Marie!

I hope my answer does not come too late!
I have asked the same question as you on the list some years ago and I got several answers.
Here is the very straightforward protocol I finally used on my EPON sections of intestine and it worked, although the staining was not very intense.
I didnt really spend much time to improve the method though.

- Periodic acid 5%: 30 min at 50°C
- Schiff Reagent: 30 min at 50°C
- Post-staining: Azur II mix (it is a 1:1 mix of methylene blue and Azur II, more stable than methylene alone): 20 min RT
(section thickness: 300 nm)

Additionally, one person told me he stained the glycogen using reduced osmium.
Another one gave me this reference:
Shroeder et al. (1980) "An established routine method for differential staining of epoxy-embedded tissue sections"
Microscopia Acta 83,111-116

Best of luck

Stéphane



----- Original Message ----
X-from: "abrun-at-hsc.unt.edu" {abrun-at-hsc.unt.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, February 25, 2009 4:36:56 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both abrun-at-hsc.unt.edu as well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abrun-at-hsc.unt.edu
Name: Anne-Marie Brun

Organization: UNT HSC at Fort Worth Texas 76107, USA

Title-Subject: [Filtered] Epon/PAS stain

Question: Has anyone ever stained for PAS on Epon sections before? If
not on Epon then what type of plastic did you use to do a PAS stain?
I know it is done on paraffin embedded material, but an investigator
wants the PAS staining done on Epon sections. ----?
Thanks for your help
Anne-Marie

  Login Host: 129.120.96.228
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:32 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3RVv3007908
6, 11 --     for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:31 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5ca6b1378b7-at-[206.69.208.22]}
6, 11 -- Date: Tue, 24 Feb 2009 21:27:29 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Epon/PAS stain
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
25, 24 -- From nizets2-at-yahoo.com Tue Mar 3 09:12:29 2009
25, 24 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231])
25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n23FCS12003890
25, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 09:12:28 -0600
25, 24 -- Received: (qmail 51519 invoked by uid 60001); 3 Mar 2009 15:12:27 -0000
25, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1236093147; bh=z/qzdZR46XI1m1L9WOz/Wx0p9Hh21jVLQKhqqfGHmTI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding; b=IqmJVoAAA7Th36MMmjI7XkYl2aKWdTP83MJW7QHqwL/4Ot1eLCvZX2wZwGa0VbCsa49VjVltDg1IJmAH/tyt+GyfOD8XQG8zphrOF5TpVUSaY5g3+oEB94+QwfiMf7wGPwRGSvXd1G2F1soWqRIENiqCrWVeEceckxxP2IpeR1w=
25, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
25, 24 -- s=s1024; d=yahoo.com;
25, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding;
25, 24 -- b=VysG0TOL7uiY9ty/TPLV1vfKhqS8YFbaGKGh2OfeqBt+KXUiTxXNbUrJvAiicx9OLToLGNx47xTESCy02rrPcZZnyw0G1jhXi6bVJSds2G5Bke33fTbz8KFUCLijx/i1rpHFvPdUKSzsY2PTwqn+3wSjY+hzNpvnDO0eIl5wdn4=;
25, 24 -- Message-ID: {22534.50843.qm-at-web110808.mail.gq1.yahoo.com}
25, 24 -- X-YMail-OSG: s5W0FP8VM1kxQqZvmsvQgWiHA0jesmtWu8g6t8WwkWQNwGUBMOo_E0XDUKMO92_KaKuX1RNR2U3Q_yWFkCD0I7XMViDYnh8lUmg._B9cdljZPExqk2VXMqcjDJHkOGFWJ0FfoMeaRe0r..JQqwWql1OYY1BDy9Ug03s.IcXX1CZ9QLlMqGC0fQf2yOEUGCyxIt9mWtuXL1F3dDsuiyn2WTJGGO57Oh2j
25, 24 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Tue, 03 Mar 2009 07:12:26 PST
25, 24 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.289.1
25, 24 -- References: {200902250336.n1P3auP7020912-at-ns.microscopy.com}
25, 24 -- Date: Tue, 3 Mar 2009 07:12:26 -0800 (PST)
25, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
25, 24 -- Subject: Re: [Microscopy] viaWWW: Epon/PAS stain
25, 24 -- To: abrun-at-hsc.unt.edu
25, 24 -- Cc: microscopy-at-microscopy.com
25, 24 -- MIME-Version: 1.0
25, 24 -- Content-Type: text/plain; charset=iso-8859-1
25, 24 -- Content-Transfer-Encoding: 8bit
25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23FCS12003890
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Tue, 3 Mar 2009 12:30:16 -0600
Subject: [Microscopy] Microscopy Today March 2009 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the March 2009 Microscopy Today table of contents. We will
close the subscription list for this issue on Thursday, March 5, 2009.
Microscopists in North America and MSA members anywhere qualify for free
subscriptions. Anyone else may subscribe for US$60 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com .

Thank you,
Ron Anderson, Technical Editor
=====================

A Colorful New Way to Look at the Nuclear Pore!
Stephen W. Carmichael, Mayo Clinic

On the Sub-Nanometer Resolution of Scanning Electron and Helium Ion
Microscopes
András E. Vladár, Michael T. Postek, and Bin Ming
Nat'l. Inst. of Standards and Technology, Gaithersburg, MD

A Versatile and Affordable Plunge Freezing Instrument for Preparing
Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM)
Linda Melanson, Gatan, Inc., Pleasanton, CA

Atomic Layer Deposition and Vapor Deposited SAMS in a CrossBeam FIB-SEM
Platform: A Path To Advanced Materials Synthesis
E. L. Principe†, C. Hartfield‡, R. Kruger‡, A. Smith‡, R. Dubois*, K.
Scammon**, B. Kempshall*** †Carl Zeiss SMT, Inc.‡ Omniprobe, Inc.*,
Colonial Metals, Inc.**, University of Central Florida, NanoSpective***

Pushing the Envelope in Atomic Force Microscopy
M. G. Heaton & J. P. Cleveland, Asylum Research, Santa Barbara, CA

Dip Pen Nanolithography: A Desktop Nanofabrication Approach Using
High-Throughput Flexible Nanopatterning
Jason Haaheim and Omkar A. Nafday, NanoInk Inc.

A Compact Field Emission SEM for Low Voltage Imaging
Jim Rynne, Novelx, Inc., Lafayette, CA

TEM Sample Preparation: An Interdisciplinary Website
J. Ayache1, L. Beaunier2, J. Boumendil3, G. Ehret4 and D. Laub5,
1.CNRS-UMR, Villejuif, France; 2.CNRS-UPR, Paris; 3.U.Claude
Bernard-Lyon, Villeurbanne, France; 4 Institut Physique et Chimie des
Matériaux de Strasbourg, France; 5.EPFL-CIME, Lausanne, Switzerland

Microparticles/Exosomes: Isolation and TEM Analysis
Natalie Bauer,Jyoti Rai, Hairu Chen, Lillianne Harris, Lalita Shevde,
Tim Moore, and Judy King, U. of South Alabama, Mobile, AL

New Ultra-Thin Pure Silicon Window Grids for Transmission Electron
Microscopy Samples
James Roussie, TEMwindows.com, Rochester, NY

Preparing Biological Samples for Analysis by High Vacuum Techniques
S.G. Ostrowski, T.L. Paxon, L. Denault, KP. McEvoy, and V.S.
Smentkowski, General Electric Global Research Ctr., Niskayuna, NY

Pioneers in Optics: Friedrich Johann Karl Becke and Anders Jöns Ångström
Michael W. Davidson, National High Magnetic Field Laboratory, The
Florida State University, Tallahassee, FL

Microscopy-101: Glass Knives vs. Diamond Knives
Lou Ann Miller, Center for Microscopic Imaging, Veterinary Medicine,
University of Illinois

Industry News

NetNotes
CHEMICALS - acrolein storage
CHEMICALS – uranyl acetate safety
CHEMICALS- cacodylate safety
SPECIMEN PREPARATION - sand
SPECIMEN PREPARATION - etching resin
SPECIMEN PREPARATION – plan-view TEM samples
SPECIMEN PREPARATION – stopping points
MICROTOMY - histo knife
IMMUNOCYTOCHEMISTRY – colloidal gold
IMMUNOCYTOCHEMISTRY – choice of secondary antibodies
SEM – current state of the art for biological specimens
SEM – cooling
EDS – Beryllium and copper
EDX – Sn and Pb ratios

Dear Abbe

Advertiser's Index


==============================Original Headers==============================
21, 17 -- From randerson20-at-tampabay.rr.com Tue Mar 3 12:30:16 2009
21, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122])
21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23IUFoK030150
21, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Mar 2009 12:30:16 -0600
21, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
21, 17 -- by hrndva-omta06.mail.rr.com with ESMTP
21, 17 -- id {20090303183015.NIVB6596.hrndva-omta06.mail.rr.com-at-[127.0.0.1]}
21, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Mar 2009 18:30:15 +0000
21, 17 -- Message-ID: {49AD772F.3090203-at-tampabay.rr.com}
21, 17 -- Date: Tue, 03 Mar 2009 13:30:07 -0500
21, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
21, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
21, 17 -- MIME-Version: 1.0
21, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
21, 17 -- Subject: Microscopy Today March 2009 Table of Contents
21, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed
21, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: sksears-at-eps.mcgill.ca
Date: Tue, 3 Mar 2009 15:57:01 -0600
Subject: [Microscopy] viaWWW: Flooring for Cryo-(S)TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sksears-at-eps.mcgill.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sksears-at-eps.mcgill.ca
Name: Kelly

Organization: McGill University

Title-Subject: [Filtered] Flooring for Cryo-(S)TEM

Question: We are installing a new cryo-(S)TEM and I would appreciate
any suggestions for an appropriate floor covering that is resistant
to liquid nitrogen spills. Thanks.

Login Host: 132.216.43.226
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Mar 3 15:57:01 2009
6, 11 -- Received: from [206.69.208.26] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23LuvId019413
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 15:57:00 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p0624080cc5d35818f3ba-at-[206.69.208.26]}
6, 11 -- Date: Tue, 3 Mar 2009 15:56:57 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: sksears-at-eps.mcgill.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Flooring for Cryo-(S)TEM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Tue, 3 Mar 2009 16:34:43 -0600
Subject: [Microscopy] Re: viaWWW: Flooring for Cryo-(S)TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kelly,
We did polished concrete floors for our entire new facility. No
maintenance (no sealers or waxes necessary) and it is chemical and
LN2 resistant.

Looks like glass but not slippery.
john

At 02:08 PM 3/3/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
7, 22 -- From donovan-at-uoregon.edu Tue Mar 3 16:34:43 2009
7, 22 -- Received: from smtp.uoregon.edu (mserv5.uoregon.edu [128.223.142.42])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23MYfes001641
7, 22 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 16:34:42 -0600
7, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
7, 22 -- (authenticated bits=0)
7, 22 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n23MYb9A008944
7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
7, 22 -- Tue, 3 Mar 2009 14:34:37 -0800
7, 22 -- Message-Id: {200903032234.n23MYb9A008944-at-smtp.uoregon.edu}
7, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
7, 22 -- Date: Tue, 03 Mar 2009 14:34:33 -0800
7, 22 -- To: sksears-at-eps.mcgill.ca
7, 22 -- From: John Donovan {donovan-at-uoregon.edu}
7, 22 -- Subject: Re: [Microscopy] viaWWW: Flooring for Cryo-(S)TEM
7, 22 -- Cc: microscopy-at-microscopy.com
7, 22 -- In-Reply-To: {200903032208.n23M8jmf027271-at-ns.microscopy.com}
7, 22 -- References: {200903032208.n23M8jmf027271-at-ns.microscopy.com}
7, 22 -- Mime-Version: 1.0
7, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
7, 22 -- X-Virus-Scanned: ClamAV 0.94.2/9065/Tue Mar 3 02:43:41 2009 on mserv5
7, 22 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Wed, 4 Mar 2009 07:50:48 -0600
Subject: [Microscopy] viaWWW: Silver enhancement for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both neh_123-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: neh_123-at-yahoo.com
Name: Nihar

Organization: U of M

Title-Subject: [Filtered] Silver enhancement for LM

Question: We are considering using silver enhancement kit from Ted
Pella to detect gold on LM sections. Does anyone have any experince
using it for LM for animal tissues such as blood, liver etc. ? I am
new to histology and would appreciate any help/protocols.

Login Host: 128.101.142.157
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Mar 3 19:33:12 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n241XBU9020011
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 19:33:11 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5d38ac0f400-at-[206.69.208.22]}
6, 11 -- Date: Tue, 3 Mar 2009 19:33:11 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: neh_123-at-yahoo.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Silver enhancement for LM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From rjcju1hjjqivflg59d80qdfi7jhbie49er-at-4ax.com Wed Mar 4 03:58:25 2009
Return-Path: {rjcju1hjjqivflg59d80qdfi7jhbie49er-at-4ax.com}
Received: from google.com (bft66.neoplus.adsl.tpnet.pl [83.28.57.66])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n249wOpO014312
for {microscopylistserverarchive-at-microscopy.com} ; Wed, 4 Mar 2009 03:58:25 -0600
Received: from [83.142.77.191] (HELO google.com)
by flatthrowajoe.org; Wed, 4 Mar 2009 10:56:08 +0100
Message-ID: {00000000AC76E92394327263}
Reply-To: Julianne Spring {17306alysha.benito-at-gmail.com}

I am sure the Pella product is good but I have found gold enhancement
far superior to silver enhancement for both LM and EM
immunocytochemistry. I like the Nanoprobes kit. Tom


Thomas E. Phillips, Ph.D.
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
Biological Sciences
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (voice)
573-882-0123 (fax)



-----Original Message-----
X-from: neh_123-at-yahoo.com [mailto:neh_123-at-yahoo.com]
Sent: Tuesday, March 03, 2009 7:34 PM
To: Phillips, Thomas E.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both neh_123-at-yahoo.com as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: neh_123-at-yahoo.com
Name: Nihar

Organization: U of M

Title-Subject: [Filtered] Silver enhancement for LM

Question: We are considering using silver enhancement kit from Ted
Pella to detect gold on LM sections. Does anyone have any experince
using it for LM for animal tissues such as blood, liver etc. ? I am
new to histology and would appreciate any help/protocols.

Login Host: 128.101.142.157
------------------------------------------------------------------------
---

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Mar 3 19:33:12 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n241XBU9020011
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009
19:33:11 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5d38ac0f400-at-[206.69.208.22]}
6, 11 -- Date: Tue, 3 Mar 2009 19:33:11 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: neh_123-at-yahoo.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Silver enhancement for LM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
17, 29 -- From PhillipsT-at-missouri.edu Wed Mar 4 07:50:47 2009
17, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24Dol2S023100
17, 29 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 07:50:47 -0600
17, 29 -- X-IronPort-Anti-Spam-Filtered: true
17, 29 -- X-IronPort-Anti-Spam-Result: ApoEAGAWrknRauUp/2dsb2JhbADHdwEBAQeHKYhMglcBAYEvBg
17, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
17, 29 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 04 Mar 2009 07:50:46 -0600
17, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
17, 29 -- Wed, 4 Mar 2009 07:50:46 -0600
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: RE: [Microscopy] viaWWW: Silver enhancement for LM
17, 29 -- Date: Wed, 4 Mar 2009 07:50:17 -0600
17, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD0616D2AA-at-UM-XMAIL06.um.umsystem.edu}
17, 29 -- In-Reply-To: {200903040133.n241XvKP021100-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: [Microscopy] viaWWW: Silver enhancement for LM
17, 29 -- Thread-Index: AcmcaUrFTXggUwi/RLyuXmDDW9jcXAAZrBRg
17, 29 -- References: {200903040133.n241XvKP021100-at-ns.microscopy.com}
17, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
17, 29 -- To: {neh_123-at-yahoo.com} , {microscopy-at-microscopy.com}
17, 29 -- X-OriginalArrivalTime: 04 Mar 2009 13:50:46.0771 (UTC) FILETIME=[38416430:01C99CD0]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24Dol2S023100
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 4 Mar 2009 09:09:21 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nikki,
And there you have it: the number one reason not to abandon a
pre-PC-controlled SEM - the PC.

I'm still servicing 30+ year old SEMs (at least one approaching 40 years)
that still do yeoman's service day after day and year after year. If your
SEM has the capabilities you need, hang on to it.

I'm sorry that I don't even have any suggestions on what to do when the
vendor runs out of DX4 spares. Perhaps get a boat so that you can moor it
to a very expensive anchor.

If the manufacturers would open their source code, someone who knows
programming might be able to update the software to a newer PC as long as
the connection is a network connection rather than proprietary driver boards
for an obsolete bus.

It is up to the users to demand a longer view towards these 6 (and 7) figure
investments.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz

DISCLAIMER: Quality Images provides both per call and full service
contracts on non-PC-controlled SEMs in the northeastern United States.


-----Original Message-----
X-from: Nicola.Weston-at-nottingham.ac.uk [mailto:Nicola.Weston-at-nottingham.ac.uk]

Sent: Tuesday, March 03, 2009 5:43 AM
To: kenconverse-at-qualityimages.biz


Hi
I think I should clarify that we have a service contract & good support
from FEI. My main concern is the integrated DX4 pc, which has been
upgraded as far as possible. So far we have been able to buy parts from
FEI but I am worried about future sourcing of spares for our system as
parts are no longer made.
It's good to know about second hand supplies from elsewhere though,
thanks for all your replies.

Regards
Nikki







Nikki

Are you saying that FEI will not sell you the parts?

regards,

Jim


} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2
Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From:
Nicola.Weston-at-nottingham.ac.uk } Reply-to:
Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver"
{microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users }
Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Hello
} We have a FEI XL30 ESEM-FEG dating back a few years now and are }
beginning to encounter problems with sourcing spare parts. Just }
wondering if anyone else out there is running an xl30 esem and if so are
} you having similar problems now they are no longer made,or does
everyone } have a Quanta these days?
}
} Thanks
} Nikki Weston
} School M3
} University of Nottingham

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009
18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk
[128.243.44.5])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n23Ae7QU031210
18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 04:40:07
-0600
18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
18, 32 -- id 1LeS1J-0007KK-Tf
18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42
+0000
18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by
SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000
18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
18, 32 -- Content-class: urn:content-classes:message
18, 32 -- MIME-Version: 1.0
18, 32 -- Content-Type: text/plain;
18, 32 -- charset="us-ascii"
18, 32 -- Subject: Esem users
18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000
18, 32 -- Message-ID:
{1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk}
18, 32 -- X-MS-Has-Attach:
18, 32 -- X-MS-TNEF-Correlator:
18, 32 -- Thread-Topic: Esem users
18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw==
18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
18, 32 -- To: {microscopy-at-microscopy.com}
18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC)
FILETIME=[37B53760:01C99BEC]
18, 32 -- X-UoN-MailScanner-Information: Please contact
staff-it-helpline-at-nottingham.ac.uk for more information
18, 32 -- X-UoN-MailScanner: Found to be clean
18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
18, 32 -- X-Spam-Status: No
18, 32 -- Content-Transfer-Encoding: 8bit
18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n23Ae7QU031210
==============================End of - Headers==============================




==============================Original Headers==============================
36, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 09:09:21 2009
36, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24F9Kcg008063
36, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 09:09:20 -0600
36, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
36, 26 -- with ESMTP
36, 26 -- id {20090304150920.KQDP12334.cdptpa-omta03.mail.rr.com-at-Ken} ;
36, 26 -- Wed, 4 Mar 2009 15:09:20 +0000
36, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
36, 26 -- To: {Nicola.Weston-at-nottingham.ac.uk} ,
36, 26 -- "MSA Listserver" {microscopy-at-microscopy.com}
36, 26 -- Subject: RE: [Microscopy] Esem users
36, 26 -- Date: Wed, 4 Mar 2009 10:09:13 -0500
36, 26 -- Message-ID: {4A77CC536B84427DAE155946EF1CD301-at-Ken}
36, 26 -- MIME-Version: 1.0
36, 26 -- Content-Type: text/plain;
36, 26 -- charset="us-ascii"
36, 26 -- X-Priority: 3 (Normal)
36, 26 -- X-MSMail-Priority: Normal
36, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
36, 26 -- Importance: Normal
36, 26 -- Thread-Index: Acmb7OTN0pOE7cqsTp64DQ8z7DqyEgA6/3Eg
36, 26 -- In-Reply-To: {200903031043.n23AhSTB002816-at-ns.microscopy.com}
36, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
36, 26 -- Content-Transfer-Encoding: 8bit
36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24F9Kcg008063
==============================End of - Headers==============================




From: James.Passmore-at-sealedair.com
Date: Wed, 4 Mar 2009 11:14:09 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nikki, how embedded is that PC? How peculiar is it?

I would think if you have a decent computer person around, you could
upgrade on your own. There could be timing issues and you might need
support for an ISA slot or two, but you should be able to do a lot. I
would not count on the manufacturer for help.

We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a
1800 MHz AMD running Windows 2000. The manufacturer cautioned us along
the way and would not guarantee that our upgrade would work. They had
not tested our exact hardware and could not guarantee success. But
neither did they promise failure. The upgrade worked fine. We do know
that the software breaks when we upgrade to Windows XP, so we are maxed
out with the OS if not the hardware. Fortunately, the hardware is
current and stable and snappy as it is.

If you do need a replacement DX4, they are still out there on the
market. I found several links to ones. I might even have one gathering
dust at home. I know I have several older, working PCs with ISA slots.

Warren S.


-----Original Message-----
X-from: kenconverse-at-qualityimages.biz
[mailto:kenconverse-at-qualityimages.biz]
Sent: Wednesday, March 04, 2009 9:10 AM
To: wesaia-at-iastate.edu

I'll second Warren's comments. I recently had an older, unsupported
instrument whose computer died. I was able put together a system that
revived the instrument. As he said, one big problem was ISA slot
requirements (I needed 2!). Also like Warren, my ultimate limiting factor
was that drivers wouldn't work with anything newer than Win2000, but that
was definitely better the WinNT I started with! I ended up with a 3GHz
Pentium 4 and plenty of RAM running Win2000, all in a PC with 2 ISA slots.

Boards with ISA slots are increasingly rare, but there are some vendors
that concentrate on industrial applications. They have limited support for
ISA slots, but the down side is their parts are typically expensive
compared to off-the-shelf consumer items. Anyone can email me if they want
the most economical source I found.

So It may not be easy, but you likely have some possibilities.

Jim

----------------------------------------------
Jim Passmore
Research Associate
Sealed Air Corporation
james.passmore-at-sealedair.com
----------------------------------------------




wesaia-at-iastate.edu wrote on 03/04/2009 10:29:55 AM:

}
}
}
}
----------------------------------------------------------------------------

} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------

}
} Nikki, how embedded is that PC? How peculiar is it?
}
} I would think if you have a decent computer person around, you could
} upgrade on your own. There could be timing issues and you might need
} support for an ISA slot or two, but you should be able to do a lot. I
} would not count on the manufacturer for help.
}
} We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a
} 1800 MHz AMD running Windows 2000. The manufacturer cautioned us along
} the way and would not guarantee that our upgrade would work. They had
} not tested our exact hardware and could not guarantee success. But
} neither did they promise failure. The upgrade worked fine. We do know
} that the software breaks when we upgrade to Windows XP, so we are maxed
} out with the OS if not the hardware. Fortunately, the hardware is
} current and stable and snappy as it is.
}
} If you do need a replacement DX4, they are still out there on the
} market. I found several links to ones. I might even have one gathering
} dust at home. I know I have several older, working PCs with ISA slots.
}
} Warren S.
}
}


==============================Original Headers==============================
13, 15 -- From James.Passmore-at-sealedair.com Wed Mar 4 11:14:09 2009
13, 15 -- Received: from dunnotesgw01.sealedair.com (dunnotesgw01.sealedair.com [165.225.194.17])
13, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24HE89m007005
13, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:14:08 -0600
13, 15 -- In-Reply-To: {200903041529.n24FTt66025164-at-ns.microscopy.com}
13, 15 -- Subject: Re: [Microscopy] Esem users
13, 15 -- To: Microscopy-at-microscopy.com
13, 15 -- X-Mailer: Lotus Notes Release 7.0.2 September 26, 2006
13, 15 -- Message-ID: {OF2BBE5F7B.9110BFAD-ON8525756F.005BF519-8525756F.005EAD1B-at-sealedair.com}
13, 15 -- From: James.Passmore-at-sealedair.com
13, 15 -- Date: Wed, 4 Mar 2009 12:08:56 -0500
13, 15 -- X-MIMETrack: Serialize by Router on DUNNOTESGW01/SAC(Release 7.0.2|September 26, 2006) at
13, 15 -- 03/04/2009 12:14:14 PM
13, 15 -- MIME-Version: 1.0
13, 15 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 4 Mar 2009 12:01:49 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just an echo of Ken's thoughts here. I guess I'm starting to qualify as
an "old timer" at this point, but, at the risk of sounding like a
Luddite, there is much to be said for an older style of non- or
less-computer dominated microscope operations.

The rush to make everything controllable by computers through GUI's
looks sexy on the monitors and can often seem to make things run more
smoothly, but I can tell you from experience that some of the most
reliable and fastest operating scopes were pre-computer-frenzy day
machines (I think they're called "tools" now). Think Hitachi S570,
which is fast and reliable and has accurate autofocus and
contrast/brightness functions that are faster than I could adjust them
by hand.

Sometimes I can believe that making everything run through a computer
has less to do with efficiency and reliability than with being forced to
appear to be as "advanced" as the competition. Do you really need a
mouse and software to desaturate a filament/tip to center the halo?

I have heard from service people that it's often the computers that make
their lives miserable. I also know that our most computer-dependent
instruments are our most maintenance intensive, often involving
time-consuming software reinstalls and problems with incompatibilities
with other programs, networking issues, etc. Plus, tracking down a
weird problem can be diabolically difficult if it's hard to tell if it's
caused by software, computer hardware, or microscope system hardware.
Our service support folks are sometimes nothing short of superhuman when
it comes to their dedication and ingenuity at tracking down and fixing
problems, but I bet sometimes it's a lot harder than it would have to
be.

There is definitely a place for computerization of parts of microscope
operation. Preset tip warm-up times are very nice in multi-user
facilities like ours, for example. Motorized stage limits set by
software for various types of holders are great. Automated tomography
packages are super.

But is it really an all or nothing proposition when it comes to computer
controlled operations? Maybe so. Not being an engineer, I'm not sure.

My two shares of AIG.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: kenconverse-at-qualityimages.biz
[mailto:kenconverse-at-qualityimages.biz]
Sent: Wednesday, March 04, 2009 9:11 AM
To: Tindall, Randy D.

Hi Nikki,
And there you have it: the number one reason not to abandon a
pre-PC-controlled SEM - the PC.

I'm still servicing 30+ year old SEMs (at least one approaching 40
years)
that still do yeoman's service day after day and year after year. If
your
SEM has the capabilities you need, hang on to it.

I'm sorry that I don't even have any suggestions on what to do when the
vendor runs out of DX4 spares. Perhaps get a boat so that you can moor
it
to a very expensive anchor.

If the manufacturers would open their source code, someone who knows
programming might be able to update the software to a newer PC as long
as
the connection is a network connection rather than proprietary driver
boards
for an obsolete bus.

It is up to the users to demand a longer view towards these 6 (and 7)
figure
investments.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz

DISCLAIMER: Quality Images provides both per call and full service
contracts on non-PC-controlled SEMs in the northeastern United States.


-----Original Message-----
X-from: Nicola.Weston-at-nottingham.ac.uk
[mailto:Nicola.Weston-at-nottingham.ac.uk]

Sent: Tuesday, March 03, 2009 5:43 AM
To: kenconverse-at-qualityimages.biz


Hi
I think I should clarify that we have a service contract & good support
from FEI. My main concern is the integrated DX4 pc, which has been
upgraded as far as possible. So far we have been able to buy parts from
FEI but I am worried about future sourcing of spares for our system as
parts are no longer made.
It's good to know about second hand supplies from elsewhere though,
thanks for all your replies.

Regards
Nikki







Nikki

Are you saying that FEI will not sell you the parts?

regards,

Jim


} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2
Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From:
Nicola.Weston-at-nottingham.ac.uk } Reply-to:
Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver"
{microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users }
Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Hello
} We have a FEI XL30 ESEM-FEG dating back a few years now and are }
beginning to encounter problems with sourcing spare parts. Just }
wondering if anyone else out there is running an xl30 esem and if so are
} you having similar problems now they are no longer made,or does
everyone } have a Quanta these days?
}
} Thanks
} Nikki Weston
} School M3
} University of Nottingham

This message has been checked for viruses but the contents of an
attachment
may still contain software viruses, which could damage your computer
system:
you are advised to perform your own checks. Email communications with
the
University of Nottingham may be monitored as permitted by UK
legislation.



==============================Original
Headers==============================
18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009
18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk
[128.243.44.5])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id
n23Ae7QU031210
18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009
04:40:07
-0600
18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
18, 32 -- id 1LeS1J-0007KK-Tf
18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42
+0000
18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231])
by
SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000
18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
18, 32 -- Content-class: urn:content-classes:message
18, 32 -- MIME-Version: 1.0
18, 32 -- Content-Type: text/plain;
18, 32 -- charset="us-ascii"
18, 32 -- Subject: Esem users
18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000
18, 32 -- Message-ID:
{1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk}
18, 32 -- X-MS-Has-Attach:
18, 32 -- X-MS-TNEF-Correlator:
18, 32 -- Thread-Topic: Esem users
18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw==
18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
18, 32 -- To: {microscopy-at-microscopy.com}
18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC)
FILETIME=[37B53760:01C99BEC]
18, 32 -- X-UoN-MailScanner-Information: Please contact
staff-it-helpline-at-nottingham.ac.uk for more information
18, 32 -- X-UoN-MailScanner: Found to be clean
18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
18, 32 -- X-Spam-Status: No
18, 32 -- Content-Transfer-Encoding: 8bit
18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n23Ae7QU031210
==============================End of -
Headers==============================




==============================Original
Headers==============================
36, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 09:09:21 2009
36, 26 -- Received: from cdptpa-omtalb.mail.rr.com
(cdptpa-omtalb.mail.rr.com [75.180.132.120])
36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n24F9Kcg008063
36, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
09:09:20 -0600
36, 26 -- Received: from Ken ([72.227.111.133]) by
cdptpa-omta03.mail.rr.com
36, 26 -- with ESMTP
36, 26 -- id
{20090304150920.KQDP12334.cdptpa-omta03.mail.rr.com-at-Ken} ;
36, 26 -- Wed, 4 Mar 2009 15:09:20 +0000
36, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
36, 26 -- To: {Nicola.Weston-at-nottingham.ac.uk} ,
36, 26 -- "MSA Listserver" {microscopy-at-microscopy.com}
36, 26 -- Subject: RE: [Microscopy] Esem users
36, 26 -- Date: Wed, 4 Mar 2009 10:09:13 -0500
36, 26 -- Message-ID: {4A77CC536B84427DAE155946EF1CD301-at-Ken}
36, 26 -- MIME-Version: 1.0
36, 26 -- Content-Type: text/plain;
36, 26 -- charset="us-ascii"
36, 26 -- X-Priority: 3 (Normal)
36, 26 -- X-MSMail-Priority: Normal
36, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
36, 26 -- Importance: Normal
36, 26 -- Thread-Index: Acmb7OTN0pOE7cqsTp64DQ8z7DqyEgA6/3Eg
36, 26 -- In-Reply-To: {200903031043.n23AhSTB002816-at-ns.microscopy.com}
36, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
36, 26 -- Content-Transfer-Encoding: 8bit
36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n24F9Kcg008063
==============================End of -
Headers==============================



==============================Original Headers==============================
53, 30 -- From TindallR-at-missouri.edu Wed Mar 4 12:01:49 2009
53, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
53, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24I1mE2021972
53, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:01:48 -0600
53, 30 -- X-IronPort-Anti-Spam-Filtered: true
53, 30 -- X-IronPort-Anti-Spam-Result: ApoEALVQrknRauUo/2dsb2JhbADIUAEJhxyITIJJDoExBg
53, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
53, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 04 Mar 2009 12:01:47 -0600
53, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
53, 30 -- Wed, 4 Mar 2009 12:01:48 -0600
53, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
53, 30 -- Content-class: urn:content-classes:message
53, 30 -- MIME-Version: 1.0
53, 30 -- Content-Type: text/plain;
53, 30 -- charset="us-ascii"
53, 30 -- Subject: RE: [Microscopy] RE: Esem users
53, 30 -- Date: Wed, 4 Mar 2009 12:00:54 -0600
53, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EB4-at-UM-XMAIL08.um.umsystem.edu}
53, 30 -- In-Reply-To: {200903041510.n24FAjHL009692-at-ns.microscopy.com}
53, 30 -- X-MS-Has-Attach:
53, 30 -- X-MS-TNEF-Correlator:
53, 30 -- Thread-Topic: [Microscopy] RE: Esem users
53, 30 -- Thread-Index: Acmc22XkIZRLzLkQRy+gQeDoJDUDiwADWYvA
53, 30 -- References: {200903041510.n24FAjHL009692-at-ns.microscopy.com}
53, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
53, 30 -- To: {kenconverse-at-qualityimages.biz}
53, 30 -- Cc: {microscopy-at-microscopy.com}
53, 30 -- X-OriginalArrivalTime: 04 Mar 2009 18:01:48.0171 (UTC) FILETIME=[498CA5B0:01C99CF3]
53, 30 -- Content-Transfer-Encoding: 8bit
53, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24I1mE2021972
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 4 Mar 2009 12:20:40 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Allow me to chime in with a "hear hear, bravo".
I agree completely. And anytime I've asked company reps why they're
so insistent on shoving computerized everything down our throats,
they always say "It's what our customers want." Except I've never
heard a customer say they want everything computerized, needed or
helpful or not.
Nor have I ever understood the advantage to having a mouse (or other
control) tell a GUI to tell a computer OS to tell a circuit to tell
another circuit to reduce the (e.g.) objective lens by X amount when
I could do as easily by turning a knob to adjust the lens-control
circuit. With less chance of programming glitches, bugs, and all the
extra failure modes.
Maybe we should start a Luddites microscopy club.

Phil

} Just an echo of Ken's thoughts here. I guess I'm starting to qualify as
} an "old timer" at this point, but, at the risk of sounding like a
} Luddite, there is much to be said for an older style of non- or
} less-computer dominated microscope operations.
}
} The rush to make everything controllable by computers through GUI's
} looks sexy on the monitors and can often seem to make things run more
} smoothly, but I can tell you from experience that some of the most
} reliable and fastest operating scopes were pre-computer-frenzy day
} machines (I think they're called "tools" now). Think Hitachi S570,
} which is fast and reliable and has accurate autofocus and
} contrast/brightness functions that are faster than I could adjust them
} by hand.
}
} Sometimes I can believe that making everything run through a computer
} has less to do with efficiency and reliability than with being forced to
} appear to be as "advanced" as the competition. Do you really need a
} mouse and software to desaturate a filament/tip to center the halo?
}
} I have heard from service people that it's often the computers that make
} their lives miserable. I also know that our most computer-dependent
} instruments are our most maintenance intensive, often involving
} time-consuming software reinstalls and problems with incompatibilities
} with other programs, networking issues, etc. Plus, tracking down a
} weird problem can be diabolically difficult if it's hard to tell if it's
} caused by software, computer hardware, or microscope system hardware.
} Our service support folks are sometimes nothing short of superhuman when
} it comes to their dedication and ingenuity at tracking down and fixing
} problems, but I bet sometimes it's a lot harder than it would have to
} be.
}
} There is definitely a place for computerization of parts of microscope
} operation. Preset tip warm-up times are very nice in multi-user
} facilities like ours, for example. Motorized stage limits set by
} software for various types of holders are great. Automated tomography
} packages are super.
}
} But is it really an all or nothing proposition when it comes to computer
} controlled operations? Maybe so. Not being an engineer, I'm not sure.
}
} My two shares of AIG.
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
} -----Original Message-----
} X-from: kenconverse-at-qualityimages.biz
} [mailto:kenconverse-at-qualityimages.biz]
} Sent: Wednesday, March 04, 2009 9:11 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] RE: Esem users
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 25 -- From oshel1pe-at-cmich.edu Wed Mar 4 12:20:40 2009
4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IKeG8003638
4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:20:40 -0600
4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n24IKVv3024268
4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:20:38 -0500
4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Wed, 4 Mar 2009 13:20:38 -0500
4, 25 -- Mime-Version: 1.0
4, 25 -- Message-Id: {f06240808c5d4755f7327-at-[141.209.160.249]}
4, 25 -- In-Reply-To: {200903041804.n24I4kLp027816-at-ns.microscopy.com}
4, 25 -- References: {200903041804.n24I4kLp027816-at-ns.microscopy.com}
4, 25 -- Date: Wed, 4 Mar 2009 13:20:36 -0500
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 25 -- Subject: Re: [Microscopy] Esem users
4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 25 -- X-OriginalArrivalTime: 04 Mar 2009 18:20:38.0144 (UTC) FILETIME=[EB10D800:01C99CF5]
4, 25 -- X-Canit-CHI2: 0.00
4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 25 -- X-Spam-Score: -3.40 () [Hold at 5.00] L_EXCH_MF,L_USD,PORN_RP_SEXY,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
4, 25 -- X-CanItPRO-Stream: default
4, 25 -- X-Canit-Stats-ID: 9837315 - bc2a61c2f0ad
4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 4 Mar 2009 12:35:52 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ooooh, count me in as a Luddite, then. We just replaced our Hitachi S-800
FESEM (a GREAT tool) with a Hitachi S-4800 FESEM (another GREAT tool). All
the youngins were excited about the coming computerization, and most said
things like "Oh, it will be easier and faster, now", and then were
astonished to find it somewhat less intuitive and a LOT slower to use. And
I can't believe how long it takes to boot!

Don't get me wrong; this instrument has five detectors and we need the
computer to mix 'em and get digital images and all, but I find everyone
using the few supplied knobs to focus, adjust brightness and contrast,
stigmate, and change mag in spite of having those functions available by
mouse and scroll bars, because it's faster. But it would sure be nice to
be able to use our microscopes even if the software got all unstable on
us!

I'd love to shut off the computer to my TEM and just let the beam shoot
straight down the column! It's then a pretty simple device.

Aloha from rainy but warm Honolulu,
Tina

} Allow me to chime in with a "hear hear, bravo".
} I agree completely. And anytime I've asked company reps why they're
} so insistent on shoving computerized everything down our throats,
} they always say "It's what our customers want." Except I've never
} heard a customer say they want everything computerized, needed or
} helpful or not.
} Nor have I ever understood the advantage to having a mouse (or other
} control) tell a GUI to tell a computer OS to tell a circuit to tell
} another circuit to reduce the (e.g.) objective lens by X amount when
} I could do as easily by turning a knob to adjust the lens-control
} circuit. With less chance of programming glitches, bugs, and all the
} extra failure modes.
} Maybe we should start a Luddites microscopy club.
}
} Phil
}
} } Just an echo of Ken's thoughts here. I guess I'm starting to qualify as
} } an "old timer" at this point, but, at the risk of sounding like a
} } Luddite, there is much to be said for an older style of non- or
} } less-computer dominated microscope operations.
} }
} } The rush to make everything controllable by computers through GUI's
} } looks sexy on the monitors and can often seem to make things run more
} } smoothly, but I can tell you from experience that some of the most
} } reliable and fastest operating scopes were pre-computer-frenzy day
} } machines (I think they're called "tools" now). Think Hitachi S570,
} } which is fast and reliable and has accurate autofocus and
} } contrast/brightness functions that are faster than I could adjust them
} } by hand.
} }
} } Sometimes I can believe that making everything run through a computer
} } has less to do with efficiency and reliability than with being forced to
} } appear to be as "advanced" as the competition. Do you really need a
} } mouse and software to desaturate a filament/tip to center the halo?
} }
} } I have heard from service people that it's often the computers that make
} } their lives miserable. I also know that our most computer-dependent
} } instruments are our most maintenance intensive, often involving
} } time-consuming software reinstalls and problems with incompatibilities
} } with other programs, networking issues, etc. Plus, tracking down a
} } weird problem can be diabolically difficult if it's hard to tell if it's
} } caused by software, computer hardware, or microscope system hardware.
} } Our service support folks are sometimes nothing short of superhuman when
} } it comes to their dedication and ingenuity at tracking down and fixing
} } problems, but I bet sometimes it's a lot harder than it would have to
} } be.
} }
} } There is definitely a place for computerization of parts of microscope
} } operation. Preset tip warm-up times are very nice in multi-user
} } facilities like ours, for example. Motorized stage limits set by
} } software for various types of holders are great. Automated tomography
} } packages are super.
} }
} } But is it really an all or nothing proposition when it comes to computer
} } controlled operations? Maybe so. Not being an engineer, I'm not sure.
} }
} } My two shares of AIG.
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} } Week&NavType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }
} }
} }
} } -----Original Message-----
} } X-from: kenconverse-at-qualityimages.biz
} } [mailto:kenconverse-at-qualityimages.biz]
} } Sent: Wednesday, March 04, 2009 9:11 AM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] RE: Esem users
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } ----
} }
} } Hi Nikki,
} } And there you have it: the number one reason not to abandon a
} } pre-PC-controlled SEM - the PC.
} }
} } I'm still servicing 30+ year old SEMs (at least one approaching 40
} } years)
} } that still do yeoman's service day after day and year after year. If
} } your
} } SEM has the capabilities you need, hang on to it.
} }
} } I'm sorry that I don't even have any suggestions on what to do when the
} } vendor runs out of DX4 spares. Perhaps get a boat so that you can moor
} } it
} } to a very expensive anchor.
} }
} } If the manufacturers would open their source code, someone who knows
} } programming might be able to update the software to a newer PC as long
} } as
} } the connection is a network connection rather than proprietary driver
} } boards
} } for an obsolete bus.
} }
} } It is up to the users to demand a longer view towards these 6 (and 7)
} } figure
} } investments.
} }
} } Ken Converse
} } owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} } DISCLAIMER: Quality Images provides both per call and full service
} } contracts on non-PC-controlled SEMs in the northeastern United States.
} }
} }
} } -----Original Message-----
} } X-from: Nicola.Weston-at-nottingham.ac.uk
} } [mailto:Nicola.Weston-at-nottingham.ac.uk]
} }
} } Sent: Tuesday, March 03, 2009 5:43 AM
} } To: kenconverse-at-qualityimages.biz
} } Subject: [Microscopy] Esem users
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } ----
} }
} }
} } Hi
} } I think I should clarify that we have a service contract & good support
} } from FEI. My main concern is the integrated DX4 pc, which has been
} } upgraded as far as possible. So far we have been able to buy parts from
} } FEI but I am worried about future sourcing of spares for our system as
} } parts are no longer made.
} } It's good to know about second hand supplies from elsewhere though,
} } thanks for all your replies.
} }
} } Regards
} } Nikki
} }
} }
} }
} }
} }
} }
} }
} } Nikki
} }
} } Are you saying that FEI will not sell you the parts?
} }
} } regards,
} }
} } Jim
} }
} }
} } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2
} } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From:
} } Nicola.Weston-at-nottingham.ac.uk } Reply-to:
} } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver"
} } {microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users }
} } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } } X-lewp: MicroscopyListSpam NAGS
} } }
} } }
} } }
} } }
} } }
} } ------------------------------------------------------------------------
} } ----
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ------------------------------------------------------------------------
} } ----
} } }
} } } Hello
} } } We have a FEI XL30 ESEM-FEG dating back a few years now and are }
} } beginning to encounter problems with sourcing spare parts. Just }
} } wondering if anyone else out there is running an xl30 esem and if so are
} } } you having similar problems now they are no longer made,or does
} } everyone } have a Quanta these days?
} } }
} } } Thanks
} } } Nikki Weston
} } } School M3
} } } University of Nottingham
}
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576
}
} ==============================Original Headers==============================
} 4, 25 -- From oshel1pe-at-cmich.edu Wed Mar 4 12:20:40 2009
} 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
} 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IKeG8003638
} 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:20:40 -0600
} 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
} 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n24IKVv3024268
} 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:20:38 -0500
} 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
} 4, 25 -- Wed, 4 Mar 2009 13:20:38 -0500
} 4, 25 -- Mime-Version: 1.0
} 4, 25 -- Message-Id: {f06240808c5d4755f7327-at-[141.209.160.249]}
} 4, 25 -- In-Reply-To: {200903041804.n24I4kLp027816-at-ns.microscopy.com}
} 4, 25 -- References: {200903041804.n24I4kLp027816-at-ns.microscopy.com}
} 4, 25 -- Date: Wed, 4 Mar 2009 13:20:36 -0500
} 4, 25 -- To: Microscopy-at-microscopy.com
} 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
} 4, 25 -- Subject: Re: [Microscopy] Esem users
} 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} 4, 25 -- X-OriginalArrivalTime: 04 Mar 2009 18:20:38.0144 (UTC) FILETIME=[EB10D800:01C99CF5]
} 4, 25 -- X-Canit-CHI2: 0.00
} 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
} 4, 25 -- X-Spam-Score: -3.40 () [Hold at 5.00] L_EXCH_MF,L_USD,PORN_RP_SEXY,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
} 4, 25 -- X-CanItPRO-Stream: default
} 4, 25 -- X-Canit-Stats-ID: 9837315 - bc2a61c2f0ad
} 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
7, 21 -- From tina-at-pbrc.hawaii.edu Wed Mar 4 12:35:51 2009
7, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IZoY1017543
7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:35:51 -0600
7, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
7, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n24IZgQf010366
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 21 -- Wed, 4 Mar 2009 08:35:43 -1000 (HST)
7, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n24IZft6010363;
7, 21 -- Wed, 4 Mar 2009 08:35:41 -1000 (HST)
7, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
7, 21 -- Date: Wed, 4 Mar 2009 08:35:40 -1000 (HST)
7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
7, 21 -- X-Sender: tina-at-halia
7, 21 -- To: oshel1pe-at-cmich.edu
7, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 21 -- Subject: Re: [Microscopy] Re: Esem users
7, 21 -- In-Reply-To: {200903041821.n24ILQqa005092-at-ns.microscopy.com}
7, 21 -- Message-ID: {Pine.GSO.4.21.0903040826180.10296-100000-at-halia}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Wed, 4 Mar 2009 13:04:01 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
Just an alternative perspective.

Maybe I'm just a computer geek or maybe it's the FEI interface (which
I find it simple, easy and intuitive), but even with the dedicated
knob set (which I insisted on when we bought the instrument, having
been an "analog knob feel" junkie myself), I find I only ever use the
big mag knob to zoom in or out.

The mouse based focus and stig I find effortless to use and very
responsive (it's right button click and drag (in X) to focus and
shift right click and drag (in X and Y) to stigmate).

I am completely won over by the FEI Quanta ESEM computer control much
to my surprise.
john



At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009
8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24J40vi031984
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:00 -0600
8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
8, 21 -- (authenticated bits=0)
8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n24J3x0h006753
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:04:00 -0800
8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu}
8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800
8, 21 -- To: microscopy-at-microscopy.com
8, 21 -- From: John Donovan {donovan-at-uoregon.edu}
8, 21 -- Subject: Re: [Microscopy] Esem users
8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- Mime-Version: 1.0
8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on mserv1
8, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Wed, 4 Mar 2009 13:04:32 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Personally, I've had experience working with high-end computing
equipment (Not necessarily microscopy related) and I know how
sensitive it can get. I just discoverd that my analog SEM developed a
short in the power transformer and was delivering 130V AC to the
instrument instead of the required 100V. The over-voltage would just
about kill any modern digital apparatus I could think of, but my scope
kept working until a couple of resistors on a scan amp board fried.
That's when I discovered the problem. Transformer replaced, resistors
replaced, and I'm running again!

Let's see anyone do that with a modern digital instrument...

Justin A. Kraft

On Wed, Mar 4, 2009 at 12:19 PM, {James.Passmore-at-sealedair.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I'll second Warren's comments.  I recently had an older, unsupported
} instrument whose computer died.  I was able put together a system that
} revived the instrument.  As he said, one big problem was ISA slot
} requirements (I needed 2!).  Also like Warren, my ultimate limiting factor
} was that drivers wouldn't work with anything newer than Win2000, but that
} was definitely better the WinNT I started with!  I ended up with a 3GHz
} Pentium 4 and plenty of RAM running Win2000, all in a PC with 2 ISA slots.
}
} Boards with ISA slots are increasingly rare, but there are some vendors
} that concentrate on industrial applications.  They have limited support for
} ISA slots, but the down side is their parts are typically expensive
} compared to off-the-shelf consumer items.  Anyone can email me if they want
} the most economical source I found.
}
} So It may not be easy, but you likely have some possibilities.
}
} Jim
}
} ----------------------------------------------
} Jim Passmore
} Research Associate
} Sealed Air Corporation
} james.passmore-at-sealedair.com
} ----------------------------------------------
}
}
}
}
} wesaia-at-iastate.edu wrote on 03/04/2009 10:29:55 AM:
}
} }
} }
} }
} }
} ----------------------------------------------------------------------------
}
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of
} America
} } To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
}
} }
} } Nikki, how embedded is that PC? How peculiar is it?
} }
} } I would think if you have a decent computer person around, you could
} } upgrade on your own. There could be timing issues and you might need
} } support for an ISA slot or two, but you should be able to do a lot. I
} } would not count on the manufacturer for help.
} }
} } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a
} } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us along
} } the way and would not guarantee that our upgrade would work. They had
} } not tested our exact hardware and could not guarantee success. But
} } neither did they promise failure. The upgrade worked fine. We do know
} } that the software breaks when we upgrade to Windows XP, so we are maxed
} } out with the OS if not the hardware. Fortunately, the hardware is
} } current and stable and snappy as it is.
} }
} } If you do need a replacement DX4, they are still out there on the
} } market. I found several links to ones. I might even have one gathering
} } dust at home. I know I have several older, working PCs with ISA slots.
} }
} } Warren S.
} }
} }
}
}
} ==============================Original Headers==============================
} 13, 15 -- From James.Passmore-at-sealedair.com Wed Mar  4 11:14:09 2009
} 13, 15 -- Received: from dunnotesgw01.sealedair.com (dunnotesgw01.sealedair.com [165.225.194.17])
} 13, 15 --       by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24HE89m007005
} 13, 15 --       for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:14:08 -0600
} 13, 15 -- In-Reply-To: {200903041529.n24FTt66025164-at-ns.microscopy.com}
} 13, 15 -- Subject: Re: [Microscopy]  Esem users
} 13, 15 -- To: Microscopy-at-microscopy.com
} 13, 15 -- X-Mailer: Lotus Notes Release 7.0.2 September 26, 2006
} 13, 15 -- Message-ID: {OF2BBE5F7B.9110BFAD-ON8525756F.005BF519-8525756F.005EAD1B-at-sealedair.com}
} 13, 15 -- From: James.Passmore-at-sealedair.com
} 13, 15 -- Date: Wed, 4 Mar 2009 12:08:56 -0500
} 13, 15 -- X-MIMETrack: Serialize by Router on DUNNOTESGW01/SAC(Release 7.0.2|September 26, 2006) at
} 13, 15 --  03/04/2009 12:14:14 PM
} 13, 15 -- MIME-Version: 1.0
} 13, 15 -- Content-type: text/plain; charset=US-ASCII
} ==============================End of - Headers==============================
}



--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar


==============================Original Headers==============================
8, 35 -- From kraftpiano-at-gmail.com Wed Mar 4 13:04:32 2009
8, 35 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.145])
8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24J4Vj6032541
8, 35 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:32 -0600
8, 35 -- Received: by qw-out-1920.google.com with SMTP id 14so2801649qwa.54
8, 35 -- for {microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 11:04:28 -0800 (PST)
8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 35 -- d=gmail.com; s=gamma;
8, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
8, 35 -- :date:message-id:subject:from:to:content-type
8, 35 -- :content-transfer-encoding;
8, 35 -- bh=Yxzw/cbgELnrQlaPjfslL1VLswcRT7FjewNX4QN1Ax4=;
8, 35 -- b=pJBJ+hWp6oX64tbAHbnKKBOz+3f10fno7zV7rHob+JuQIvdxXoifxvit6zPRedx+fB
8, 35 -- 1lsxsOoow62Cow7GCO7vR3qi0kmihKJQSiitbXYQV28XPT+8L7OLvJoZWBDEFLkixptP
8, 35 -- KhYoP+hztVx/qhKxmUEKEvwW/P1b7FtAXaMQo=
8, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 35 -- d=gmail.com; s=gamma;
8, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
8, 35 -- :content-type:content-transfer-encoding;
8, 35 -- b=n2T7pOUNbQ0z3MK7Vf8wAeKpbdLcnZFoq0gVf/JR42CUVGQH07J/akJnNGEKF8s5U2
8, 35 -- 0ZM1MiEfsWotvVkowRcqC+HSv8Rg5e6WLbF5694mU+HQh4FDQxRhCMqdu65fZASC1OYv
8, 35 -- EVxbHo2dzojQzh4jq2XsJGYK0aGR2QJawLqBA=
8, 35 -- MIME-Version: 1.0
8, 35 -- Received: by 10.224.74.68 with SMTP id t4mr413399qaj.59.1236193467961; Wed, 04
8, 35 -- Mar 2009 11:04:27 -0800 (PST)
8, 35 -- In-Reply-To: {200903041719.n24HJhqj015852-at-ns.microscopy.com}
8, 35 -- References: {200903041719.n24HJhqj015852-at-ns.microscopy.com}
8, 35 -- Date: Wed, 4 Mar 2009 14:04:27 -0500
8, 35 -- Message-ID: {25e2b0d20903041104y63268e20r6b38887a3c33540c-at-mail.gmail.com}
8, 35 -- Subject: Re: [Microscopy] Re: Esem users
8, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
8, 35 -- To: James.Passmore-at-sealedair.com, microscopy-at-microscopy.com
8, 35 -- Content-Type: text/plain; charset=ISO-8859-1
8, 35 -- Content-Transfer-Encoding: 8bit
8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24J4Vj6032541
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Wed, 4 Mar 2009 13:07:18 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

OK, I have to go along with everyone else on this one. I have a
different take on the situation, so here goes.

After I learned how to align the TEM, I kind of forgot the names of
all the controls. It became second nature to follow a routine, I knew
where to reach for the knob I needed, I didn't have to remember its
name or look to see what it was labeled. I could do it in the dark,
almost in my sleep. It was easy. All the motor movements and
kinesthetic learning things helped me 'know' where to reach and what
to do without looking. Besides, in those days it was dark, I couldn't
have read a label if I wanted to.

I recently had some experience with a 'modern' microscope. Computer
and mouse did the controlling. First, the darn computer screen was too
bright and made it hard to keep my eyes dark adapted. Second, since I
forgot the real names of the knobs I needed to manipulate, just knew
where to reach, I had a heck of a time relearning what to call them so
I could click the mouse on the correct 'button' on the computer. As
screens changed, the things I wanted were moving all over the place
and it was harder to keep track of where the control I needed was to
be found.

And here is the ultimate insult. With old age has come poor close
vision. So now, even if I knew what I was looking for in the computer
screen, I can't see it without my reading glasses. So, its glasses on
to read the screen, glasses off to look through the binocs, glasses on
to check something on the screen and back and forth. Very unhandy. In
the old days, I could run the microscope controls by the braille
method, no seeing needed, now it is not so handy.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
9, 44 -- From jkrupp-at-deltacollege.edu Wed Mar 4 13:07:17 2009
9, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
9, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24J7G9p006706
9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:07:16 -0600
9, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
9, 44 -- by localhost (Email Security Appliance) with SMTP id B1B8F213C5E_9AECBD2B
9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 18:43:30 +0000 (GMT)
9, 44 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
9, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id AF2411B6095_9AECB82F
9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 18:41:05 +0000 (GMT)
9, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
9, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
9, 44 -- with ESMTP id 45893833 for Microscopy-at-microscopy.com; Wed, 04 Mar 2009 11:03:37 -0800
9, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
9, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
9, 44 -- ESMTP id KFZW6T00.KRA for {Microscopy-at-microscopy.com} ; Wed, 4
9, 44 -- Mar 2009 10:47:18 -0800
9, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1])
9, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C27FF8F74E7A
9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:03:36 -0800 (PST)
9, 44 -- X-Virus-Scanned: amavisd-new at
9, 44 -- X-Spam-Flag: NO
9, 44 -- X-Spam-Score: -2.499
9, 44 -- X-Spam-Level:
9, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
9, 44 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
9, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
9, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
9, 44 -- with ESMTP id 0AfCIaoBDC6u for {Microscopy-at-microscopy.com} ;
9, 44 -- Wed, 4 Mar 2009 11:03:36 -0800 (PST)
9, 44 -- Received: from [172.20.3.10] (unknown [172.20.3.10])
9, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 515438F74E76
9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:03:36 -0800 (PST)
9, 44 -- Message-Id: {09F26248-15FB-472E-AFCD-4E4E1F66FB1C-at-deltacollege.edu}
9, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
9, 44 -- To: Microscopy-at-microscopy.com
9, 44 -- In-Reply-To: {200903041843.n24IhlBU030143-at-ns.microscopy.com}
9, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 44 -- Content-Transfer-Encoding: 7bit
9, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3)
9, 44 -- Subject: Re: [Microscopy] Esem users
9, 44 -- Date: Wed, 4 Mar 2009 11:03:35 -0800
9, 44 -- References: {200903041843.n24IhlBU030143-at-ns.microscopy.com}
9, 44 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Wed, 4 Mar 2009 13:18:38 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've enjoyed reading this thread, and thought I'd add something from a
slightly different perspective. After 35 years using LOM for metallography,
I recently inherited the job and the tool (an old Cambridge S200) after our
x-ray guy of 25 years was downsized (not my decision). Having never used an
SEM or contemplated x-ray microanalysis in my life, you can imagine I was a
bit overwhelmed by a control panel, never mind the science, that would make
a space-shuttle pilot cringe. I'm a little over a year into it now, and
still dreamed of how wonderful it must be to use a state of the art, mouse
controlled, LN2 free tool...until I began following this thread. I think
I'll stick with ole Betsy. I've become comfortable with her eccentricities.

Jeff Stewart
Metallographic Lab Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329
----- Original Message -----
X-from: {tina-at-pbrc.hawaii.edu}
To: {jeff-at-metallography.com}
Sent: Wednesday, March 04, 2009 1:36 PM

Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean and only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty.

Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back.

My 2 cents Canadian (~1.56 cents US),

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf




==============================Original Headers==============================
10, 19 -- From jehrman-at-mta.ca Wed Mar 4 13:18:37 2009
10, 19 -- Received: from mailgate2.mta.ca (mailgate2.mta.ca [138.73.1.142])
10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24JIaN8013366
10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:18:37 -0600
10, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:49479)
10, 19 -- by mailgate2.mta.ca (smtp.mta.ca [138.73.1.130]:25)
10, 19 -- with esmtp id 1LexXM-0004KG-G6 (Exim 4.69) for Microscopy-at-microscopy.com
10, 19 -- (return-path {jehrman-at-mta.ca} ); Wed, 04 Mar 2009 16:17:52 -0400
10, 19 -- Message-ID: {49AED2D7.3040407-at-mta.ca}
10, 19 -- Date: Wed, 04 Mar 2009 15:13:27 -0400
10, 19 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
10, 19 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
10, 19 -- MIME-Version: 1.0
10, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
10, 19 -- Subject: [Microscopy] Re: Esem users
10, 19 -- X-Enigmail-Version: 0.95.7
10, 19 -- Content-Type: text/plain; charset=ISO-8859-1
10, 19 -- Content-Transfer-Encoding: 8bit
10, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24JIaN8013366
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Wed, 4 Mar 2009 14:02:22 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Warren

this does raise one or two interesting points about computers in
microscopy.

We fairly easily upgraded the operating system on our Link EDS system
several years ago but had a lot of problems even discussing the use of
new printer drivers for our Hitachi S3000N.

I get the impression that microscope control systems and imaging are
so heavily integrated into their computers that a simple upgrade is
not possible because of the complexity of hardware, bespoke drivers,
special software and the operating system and peripherals. Maybe this
will change with 2nd and 3rd generation systems.

Analytical EDS systems which have been computerized for years seem a
bit easier because they have less integrated controls, no active
imaging and software packages seem a bit more portable.

Maybe as well as looking out for sales of microscopy spares it might
be useful to keep a couple of legacy computers for the technologies
that won't migrate onto newer computers.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: wesaia-at-iastate.edu

Yes, in response to Malcom.

Another example: one of our scopes still runs Window NT Workstation. To
upgrade to an operating system from this century will cost from
$20,000-40,000, depending on who I've talked to. Our IT people won't
touch this system. Once, back in the days of ZIP drives, our drive
failed and we couldn't get images off the scope. We couldn't get our
LAN or CD writer to work, either. When we reinstalled the drivers for
the drive, the scope went down. Took a $7000 service call to get it
running again. I actually had to send a claim to our insurance people
for that amount for "Installing ZIP drivers" (we had made an ill-advised
experiment with insurance companies vs. service contracts---don't).

So, yes also to Jim---keep your controller computers squeaky clean.
Play games and chat on your laptop.

Another, more recent, example: one scope had an intermittent alarm going
off and when it went off half the controls would lock up. No beam
brightness control and only half of the beam traverse and alignment
controls would work (x-axis only). Literally weeks of trouble shooting
went into diagnosing this, during which we could use the scope only
part-time, when the alarm wasn't going off. Finally we realized it was
the computer case speaker making the noise, not the scope. I finally
figured out how to shut off the case speaker to save our sanity (Google
is a wonderful thing), and when I did the scope quit malfunctioning.
This one left everyone from Columbia, MO to Tokyo scratching their
heads.

Our service engineer replaced a couple likely suspect components and now
things are back to normal, but we still don't know for sure what
malfunctioned. This is not a dig at the service people---ours are
stellar. It just is an example of how complicated things can be.

So I'm back to my original point: computer-controlled functions are
often really great things, as a couple posters have pointed out. But
does it have to be for everything?

Randy

-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk
[mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Wednesday, March 04, 2009 1:27 PM
To: Tindall, Randy D.

Warren

this does raise one or two interesting points about computers in
microscopy.

We fairly easily upgraded the operating system on our Link EDS system
several years ago but had a lot of problems even discussing the use of
new printer drivers for our Hitachi S3000N.

I get the impression that microscope control systems and imaging are
so heavily integrated into their computers that a simple upgrade is
not possible because of the complexity of hardware, bespoke drivers,
special software and the operating system and peripherals. Maybe this
will change with 2nd and 3rd generation systems.

Analytical EDS systems which have been computerized for years seem a
bit easier because they have less integrated controls, no active
imaging and software packages seem a bit more portable.

Maybe as well as looking out for sales of microscopy spares it might
be useful to keep a couple of legacy computers for the technologies
that won't migrate onto newer computers.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: wesaia-at-iastate.edu

Let me add another perspective on mouse verses knobs: After 36 years of
microscopy, I have never seen anyone injure their hands twiddling knobs.
However, at Intel and now Numonyx, repetitive stress injuries, also
known as musculo-skeletal injuries, or MSDs are epidemic. Tendon and
nerve injuries from the shoulders to the fingers constitute the largest
fraction of industrial injuries in the semiconductor industry. It may
seem unbelievable that with all those big factories more people get
injured pushing a mouse around and clicking than driving forklifts,
cranes, wafer robots etc. put together. These injuries are not life
threatening but are frequently career ending because they tend to take
years to heal, and the individual is basically functionally disabled. I
have experienced these injuries myself, so for me the next time I go
shopping for a new whatever, it better be run by knobs.

John Mardinly,
Numonyx

-----Original Message-----
X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
Sent: Wednesday, March 04, 2009 11:14 AM
To: MARDINLY, A

Hi all,
Just an alternative perspective.

Maybe I'm just a computer geek or maybe it's the FEI interface (which
I find it simple, easy and intuitive), but even with the dedicated
knob set (which I insisted on when we bought the instrument, having
been an "analog knob feel" junkie myself), I find I only ever use the
big mag knob to zoom in or out.

The mouse based focus and stig I find effortless to use and very
responsive (it's right button click and drag (in X) to focus and
shift right click and drag (in X and Y) to stigmate).

I am completely won over by the FEI Quanta ESEM computer control much
to my surprise.
john



At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote:
} -----------------------------------------------------------------------
-----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


==============================Original
Headers==============================
8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009
8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu
[128.223.142.40])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n24J40vi031984
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
13:04:00 -0600
8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu
[128.223.10.46])
8, 21 -- (authenticated bits=0)
8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id
n24J3x0h006753
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NOT)
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
11:04:00 -0800
8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu}
8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800
8, 21 -- To: microscopy-at-microscopy.com
8, 21 -- From: John Donovan {donovan-at-uoregon.edu}
8, 21 -- Subject: Re: [Microscopy] Esem users
8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- Mime-Version: 1.0
8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on
mserv1
8, 21 -- X-Virus-Status: Clean
==============================End of -
Headers==============================



==============================Original Headers==============================
16, 29 -- From A.MARDINLY-at-numonyx.com Wed Mar 4 14:02:21 2009
16, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24K2LVo031089
16, 29 -- for {Microscopy-at-Microscopy.com} ; Wed, 4 Mar 2009 14:02:21 -0600
16, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
16, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id F15C0144007;
16, 29 -- Wed, 4 Mar 2009 13:06:39 -0500 (EST)
16, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
16, 29 -- Wed, 4 Mar 2009 15:02:21 -0500
16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 29 -- Content-class: urn:content-classes:message
16, 29 -- MIME-Version: 1.0
16, 29 -- Content-Type: text/plain;
16, 29 -- charset="us-ascii"
16, 29 -- Subject: RE: [Microscopy] Re: Esem users
16, 29 -- Date: Wed, 4 Mar 2009 15:01:38 -0500
16, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE9490-at-EXDRESBENMX012.numonyx.local}
16, 29 -- In-Reply-To: {200903041914.n24JE3nw031358-at-ns.microscopy.com}
16, 29 -- X-MS-Has-Attach:
16, 29 -- X-MS-TNEF-Correlator:
16, 29 -- Thread-Topic: [Microscopy] Re: Esem users
16, 29 -- Thread-Index: Acmc/W1LTvDXd8D2Q7OtHKARYKs5ZQABVcug
16, 29 -- References: {200903041914.n24JE3nw031358-at-ns.microscopy.com}
16, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
16, 29 -- To: {donovan-at-uoregon.edu}
16, 29 -- Cc: {Microscopy-at-Microscopy.com}
16, 29 -- X-OriginalArrivalTime: 04 Mar 2009 20:02:21.0381 (UTC) FILETIME=[20E0E750:01C99D04]
16, 29 -- Content-Transfer-Encoding: 8bit
16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24K2LVo031089
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Wed, 4 Mar 2009 14:05:40 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to add my 2 cents into this thread.

I recently purchased a SEM for my work and having pretty much carte blanc,
I decided on a rather less followed path. I purchased an older column and
control panel that was just pre-computer computer age having excellent
optics. I then had a brand new analytical interface put on it.

This makes my base SEM rather easy to work on and keep running and a very
nice interface for computer control that comes with free upgrades for the
life of the system.

In this way, I can use whatever current computer operating system happens
to exist, get computer control (somewhat limited) on my SEM with all
components repairable and upgradable for as many years as I will ever work
on this tool.

I also have to say that this list was key in me making this choice as I've
been hearing for a long time people complaining about the embeded
computer microchips having long term maintainence problems.

dj


On Wed, 4 Mar 2009, malcolm.haswell-at-sunderland.ac.uk wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Warren
}
} this does raise one or two interesting points about computers in
} microscopy.
}
} We fairly easily upgraded the operating system on our Link EDS system
} several years ago but had a lot of problems even discussing the use of
} new printer drivers for our Hitachi S3000N.
}
} I get the impression that microscope control systems and imaging are
} so heavily integrated into their computers that a simple upgrade is
} not possible because of the complexity of hardware, bespoke drivers,
} special software and the operating system and peripherals. Maybe this
} will change with 2nd and 3rd generation systems.
}
} Analytical EDS systems which have been computerized for years seem a
} bit easier because they have less integrated controls, no active
} imaging and software packages seem a bit more portable.
}
} Maybe as well as looking out for sales of microscopy spares it might
} be useful to keep a couple of legacy computers for the technologies
} that won't migrate onto newer computers.
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscope Unit
} Faculty of Applied Sciences
} University of Sunderland
} SUNDERLAND
} SR1 3SD
} UK
}
} email: malcolm.haswell-at-sunderland.ac.uk
}
}
} ----- Original Message -----
} X-from: wesaia-at-iastate.edu
} Date: Wednesday, March 4, 2009 3:33 pm
} Subject: [Microscopy] Esem users
}
} }
} }
} }
} } -------------------------------------------------------------------
} } ---------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } AmericaTo Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserverOn-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } ----------------------------------------------------------------
} }
} } Nikki, how embedded is that PC? How peculiar is it?
} }
} } I would think if you have a decent computer person around, you could
} } upgrade on your own. There could be timing issues and you might need
} } support for an ISA slot or two, but you should be able to do a
} } lot. I
} } would not count on the manufacturer for help.
} }
} } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3
} } to a
} } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us
} along
} } the way and would not guarantee that our upgrade would work. They had
} } not tested our exact hardware and could not guarantee success. But
} } neither did they promise failure. The upgrade worked fine. We do know
} } that the software breaks when we upgrade to Windows XP, so we are
} } maxedout with the OS if not the hardware. Fortunately, the
} } hardware is
} } current and stable and snappy as it is.
} }
} } If you do need a replacement DX4, they are still out there on the
} } market. I found several links to ones. I might even have one
} gathering
} } dust at home. I know I have several older, working PCs with ISA
} } slots.
} }
} } Warren S.
} }
} }
} } -----Original Message-----
} } X-from: kenconverse-at-qualityimages.biz
} } [mailto:kenconverse-at-qualityimages.biz]
} } Sent: Wednesday, March 04, 2009 9:10 AM
} } To: wesaia-at-iastate.edu
} } Subject: [Microscopy] RE: Esem users
} }
} } -------------------------------------------------------------------
} } -----
} }
} } Hi Nikki,
} } And there you have it: the number one reason not to abandon a
} } pre-PC-controlled SEM - the PC.
} }
} } I'm still servicing 30+ year old SEMs (at least one approaching 40
} } years)
} } that still do yeoman's service day after day and year after year. If
} } your
} } SEM has the capabilities you need, hang on to it.
} }
} } I'm sorry that I don't even have any suggestions on what to do
} } when the
} } vendor runs out of DX4 spares. Perhaps get a boat so that you can
} } moorit
} } to a very expensive anchor.
} }
} } If the manufacturers would open their source code, someone who knows
} } programming might be able to update the software to a newer PC as
} long
} } as
} } the connection is a network connection rather than proprietary driver
} } boards
} } for an obsolete bus.
} }
} } It is up to the users to demand a longer view towards these 6 (and 7)
} } figure
} } investments.
} }
} } Ken Converse
} } owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} } DISCLAIMER: Quality Images provides both per call and full service
} } contracts on non-PC-controlled SEMs in the northeastern United
} States.
} }
} }
} } -----Original Message-----
} } X-from: Nicola.Weston-at-nottingham.ac.uk
} } [mailto:Nicola.Weston-at-nottingham.ac.uk]
} }
} } Hi
} } I think I should clarify that we have a service contract & good
} } supportfrom FEI. My main concern is the integrated DX4 pc, which
} } has been
} } upgraded as far as possible. So far we have been able to buy parts
} } fromFEI but I am worried about future sourcing of spares for our
} } system as
} } parts are no longer made.
} } It's good to know about second hand supplies from elsewhere though,
} } thanks for all your replies.
} }
} } Regards
} } Nikki
} }
} }
} }
} } Nikki
} }
} } Are you saying that FEI will not sell you the parts?
} }
} } regards,
} }
} } Jim
} }
} }
} } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date:
} } Mon, 2
} } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu }
} } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to:
} } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy
} } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy]
} } ESEM users }
} } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } } X-lewp: MicroscopyListSpam NAGS
} } }
} } -------------------------------------------------------------------
} } -----
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html }
} } -------------------------------------------------------------------
} } -----
} } }
} } } Hello
} } } We have a FEI XL30 ESEM-FEG dating back a few years now and are
} } }
} } beginning to encounter problems with sourcing spare parts. Just }
} } wondering if anyone else out there is running an xl30 esem and if
} } so are
} } } you having similar problems now they are no longer made,or does
} } everyone } have a Quanta these days?
} } }
} } } Thanks
} } } Nikki Weston
} } } School M3
} } } University of Nottingham
} }
} } This message has been checked for viruses but the contents of an
} } attachment
} } may still contain software viruses, which could damage your computer
} } system:
} } you are advised to perform your own checks. Email communications with
} } the
} } University of Nottingham may be monitored as permitted by UK
} } legislation.
} }
} }
} }
} } ==============================Original
} } Headers==============================31, 36 -- From
} } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009
} } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub-
} } 4.iastate.edu [129.186.140.14])
} } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n24FSFIW022376
} } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } 09:28:15 -0600
} } 31, 36 -- Received: from devirus-10.iastate.edu (devirus-
} } 10.iastate.edu [129.186.1.47])
} } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10)
} } with SMTP id n24FSETv025573
} } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } 09:28:14 -0600
} } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80])
} } by devirus-10.iastate.edu with smtp
} } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a;
} } 31, 36 -- Wed, 04 Mar 2009 09:10:28 -0600
} } 31, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
} } [129.186.23.85])31, 36 -- by despam-10.iastate.edu
} } (8.14.2/8.12.10) with ESMTP id n24FSD1H004703
} } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } 09:28:13 -0600
} } 31, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
} } owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } 31, 36 -- Wed, 4 Mar 2009 09:28:14 -0600
} } 31, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } 31, 36 -- Content-class: urn:content-classes:message
} } 31, 36 -- MIME-Version: 1.0
} } 31, 36 -- Content-Type: text/plain;
} } 31, 36 -- charset="us-ascii"
} } 31, 36 -- Subject: RE: [Microscopy] RE: Esem users
} } 31, 36 -- Date: Wed, 4 Mar 2009 09:28:35 -0600
} } 31, 36 -- Message-ID:
} } {16A330AC32056A40B32842EC4BB8D72703B2A1D0-at-maire.eng.iastate.edu} 31,
} 36 -- In-Reply-To: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } 31, 36 -- X-MS-Has-Attach:
} } 31, 36 -- X-MS-TNEF-Correlator:
} } 31, 36 -- Thread-Topic: [Microscopy] RE: Esem users
} } 31, 36 -- Thread-Index: Acmc21oidxgM5IUFT72HTN2CRnlTRgAATOqQ
} } 31, 36 -- References: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } 31, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} } 31, 36 -- To: {Microscopy-at-microscopy.com}
} } 31, 36 -- X-OriginalArrivalTime: 04 Mar 2009 15:28:14.0531 (UTC)
} } FILETIME=[D5CAC130:01C99CDD]31, 36 -- X-PMX-Version: 5.5.2.365749,
} } Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.151931
} } 31, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
} } Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0,
} } BODY_SIZE_7000_LESS 0, OEM_SOFTWARE_X1 0, __BOUNCE_CHALLENGE_SUBJ
} } 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0,
} } __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0,
} } __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0,
} } __PHISH_SPEAR_REASONS 0, __SANE_MSGID 0'
} } 31, 36 -- Content-Transfer-Encoding: 8bit
} } 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n24FSFIW022376
} } ==============================End of -
} } Headers==============================
}
} ==============================Original Headers==============================
} 11, 35 -- From malcolm.haswell-at-sunderland.ac.uk Wed Mar 4 13:25:31 2009
} 11, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk [157.228.29.83])
} 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24JPUZh001937
} 11, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:25:31 -0600
} 11, 35 -- Received: (qmail 4727 invoked from network); 4 Mar 2009 17:01:24 -0000
} 11, 35 -- Received: from localhost (127.0.0.1)
} 11, 35 -- by max1.sunderland.ac.uk with SMTP; 4 Mar 2009 17:01:24 -0000
} 11, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1])
} 11, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024)
} 11, 35 -- with SMTP id 03643-04 for {Microscopy-at-microscopy.com} ;
} 11, 35 -- Wed, 4 Mar 2009 17:01:13 +0000 (GMT)
} 11, 35 -- Received: (qmail 4643 invoked by uid 607); 4 Mar 2009 17:01:13 -0000
} 11, 35 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117)
} 11, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 04 Mar 2009 17:01:13 +0000
} 11, 35 -- Received: from sunderland.ac.uk (localhost [127.0.0.1])
} 11, 35 -- by hermes.sunderland.ac.uk
} 11, 35 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004))
} 11, 35 -- with ESMTP id {0KFZ0039VRA25L-at-hermes.sunderland.ac.uk} for
} 11, 35 -- Microscopy-at-microscopy.com; Wed, 04 Mar 2009 17:01:15 +0000 (GMT)
} 11, 35 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed,
} 11, 35 -- 04 Mar 2009 17:01:14 +0000 (GMT)
} 11, 35 -- Date: Wed, 04 Mar 2009 17:01:14 +0000 (GMT)
} 11, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
} 11, 35 -- Subject: Re: [Microscopy] Esem users
} 11, 35 -- To: wesaia-at-iastate.edu, Microscopy MSA {Microscopy-at-microscopy.com}
} 11, 35 -- Message-id: {add89b3988.b3988add89-at-sunderland.ac.uk}
} 11, 35 -- MIME-version: 1.0
} 11, 35 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004)
} 11, 35 -- Content-type: text/plain; charset=us-ascii
} 11, 35 -- Content-language: en
} 11, 35 -- Content-transfer-encoding: 7BIT
} 11, 35 -- Content-disposition: inline
} 11, 35 -- X-Accept-Language: en
} 11, 35 -- Priority: normal
} 11, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
9, 18 -- From dljones-at-bestweb.net Wed Mar 4 14:05:40 2009
9, 18 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43])
9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24K5ebE005833
9, 18 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:05:40 -0600
9, 18 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202])
9, 18 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 18 -- (No client certificate requested)
9, 18 -- by smtp3.bestweb.net (Postfix) with ESMTPS id C97145C67
9, 18 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:05:39 -0500 (EST)
9, 18 -- Date: Wed, 4 Mar 2009 19:37:30 +0000 (UTC)
9, 18 -- From: dljones {dljones-at-bestweb.net}
9, 18 -- To: microscopy-at-microscopy.com
9, 18 -- Subject: Re: [Microscopy] Re: Esem users
9, 18 -- In-Reply-To: {200903041928.n24JSY66008245-at-ns.microscopy.com}
9, 18 -- Message-ID: {Pine.BSF.4.64.0903041923470.32551-at-monet.bestweb.net}
9, 18 -- References: {200903041928.n24JSY66008245-at-ns.microscopy.com}
9, 18 -- MIME-Version: 1.0
9, 18 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 4 Mar 2009 14:21:54 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

This is an interesting discussion. I'm pleased to see that I'm not the only person with deep-
seated reservations about computerised instrumentation. It costs a fortune to get anything
professionally serviced down here as real expertise is often only to be found in Australia, and
by the time accomodation, travel cost, and travel time is added, a one-day visit can cost
thousands of our shrinking dollars.

Australia and New Zealand are not, in fact, all that close together!

My 1987 JEOL 840 has a totally mysterious and enigmatic CPU embedded deep within it,
being, I guess the early days of microcomputerisation, the documentation has only an
occasional reference to it.

I live in fear of it malfunctioning but am comforted somewhat by the amazing reliability of the
JEOL electronics.

If it dies or even gets sick, is that the end for the instrument?

Anybody been there with an 840?

cheers
Ritchie Sims




--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
14, 30 -- From r.sims-at-auckland.ac.nz Wed Mar 4 14:21:54 2009
14, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34])
14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24KLrs3026200
14, 30 -- for {microscopy-at-Microscopy.Com} ; Wed, 4 Mar 2009 14:21:53 -0600
14, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3EA991AA7A
14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:52 +1300 (NZDT)
14, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
14, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
14, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
14, 30 -- with ESMTP id VWtYifBHddk0 for {microscopy-at-Microscopy.Com} ;
14, 30 -- Thu, 5 Mar 2009 09:21:52 +1300 (NZDT)
14, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
14, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
14, 30 -- (No client certificate requested)
14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1F5AA1AA70
14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:51 +1300 (NZDT)
14, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
14, 30 -- To: microscopy-at-Microscopy.Com
14, 30 -- Date: Thu, 05 Mar 2009 09:24:00 +1300
14, 30 -- MIME-Version: 1.0
14, 30 -- Subject: Re: [Microscopy] Esem users
14, 30 -- Message-ID: {49AF9A30.12078.415D66-at-r.sims.auckland.ac.nz}
14, 30 -- Priority: normal
14, 30 -- In-reply-to: {200903042006.n24K6SAt008123-at-ns.microscopy.com}
14, 30 -- References: {200903042006.n24K6SAt008123-at-ns.microscopy.com}
14, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
14, 30 -- Content-type: text/plain; charset=US-ASCII
14, 30 -- Content-transfer-encoding: 7BIT
14, 30 -- Content-description: Mail message body
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Wed, 4 Mar 2009 14:40:16 -0600
Subject: [Microscopy] Fwd: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Dear Malcolm,
} I recently upgraded my Hitachi S3000N computer and it was not a problem, just cost money and required a service call, because there are things to change inside the SEM, as well as the computer and monitor. The computer has to be configured in Japan. The new system boots very fast, I got new software that is a bit nicer and I won't be obselete for another few years.
} On this thread, one of the reasons that the SEMs are all run on consumer computers, besides the fact that customers demanded it, is that it is way cheaper. Turn knobs, pots and other discrete components are expensive, now, compared to software, which gets cheaper the more systems you install.
} My two cents worth.
} Regards,
} Mary Fletcher
}
}
}
} -----Original Message-----
}
} } Date: Wed Mar 04 11:31:51 PST 2009
} } From: malcolm.haswell-at-sunderland.ac.uk
} } Subject: [Microscopy] Re: Esem users
} } To: maryflet-at-interchange.ubc.ca
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Warren
} }
} } this does raise one or two interesting points about computers in
} } microscopy.
} }
} } We fairly easily upgraded the operating system on our Link EDS system
} } several years ago but had a lot of problems even discussing the use of
} } new printer drivers for our Hitachi S3000N.
} }
} } I get the impression that microscope control systems and imaging are
} } so heavily integrated into their computers that a simple upgrade is
} } not possible because of the complexity of hardware, bespoke drivers,
} } special software and the operating system and peripherals. Maybe this
} } will change with 2nd and 3rd generation systems.
} }
} } Analytical EDS systems which have been computerized for years seem a
} } bit easier because they have less integrated controls, no active
} } imaging and software packages seem a bit more portable.
} }
} } Maybe as well as looking out for sales of microscopy spares it might
} } be useful to keep a couple of legacy computers for the technologies
} } that won't migrate onto newer computers.
} }
} } Malcolm
} }
} } Malcolm Haswell
} } Electron Microscope Unit
} } Faculty of Applied Sciences
} } University of Sunderland
} } SUNDERLAND
} } SR1 3SD
} } UK
} }
} } email: malcolm.haswell-at-sunderland.ac.uk
} }
} }
} } ----- Original Message -----
} } X-from: wesaia-at-iastate.edu
} } Date: Wednesday, March 4, 2009 3:33 pm
} } Subject: [Microscopy] Esem users
} }
} } }
} } }
} } }
} } } -------------------------------------------------------------------
} } } ---------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserverOn-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } ----------------------------------------------------------------
} } }
} } } Nikki, how embedded is that PC? How peculiar is it?
} } }
} } } I would think if you have a decent computer person around, you could
} } } upgrade on your own. There could be timing issues and you might need
} } } support for an ISA slot or two, but you should be able to do a
} } } lot. I
} } } would not count on the manufacturer for help.
} } }
} } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3
} } } to a
} } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us
} } along
} } } the way and would not guarantee that our upgrade would work. They had
} } } not tested our exact hardware and could not guarantee success. But
} } } neither did they promise failure. The upgrade worked fine. We do know
} } } that the software breaks when we upgrade to Windows XP, so we are
} } } maxedout with the OS if not the hardware. Fortunately, the
} } } hardware is
} } } current and stable and snappy as it is.
} } }
} } } If you do need a replacement DX4, they are still out there on the
} } } market. I found several links to ones. I might even have one
} } gathering
} } } dust at home. I know I have several older, working PCs with ISA
} } } slots.
} } }
} } } Warren S.
} } }
} } }
} } } -----Original Message-----
} } } X-from: kenconverse-at-qualityimages.biz
} } } [mailto:kenconverse-at-qualityimages.biz]
} } } Sent: Wednesday, March 04, 2009 9:10 AM
} } } To: wesaia-at-iastate.edu
} } } Subject: [Microscopy] RE: Esem users
} } }
} } } -------------------------------------------------------------------
} } } -----
} } }
} } } Hi Nikki,
} } } And there you have it: the number one reason not to abandon a
} } } pre-PC-controlled SEM - the PC.
} } }
} } } I'm still servicing 30+ year old SEMs (at least one approaching 40
} } } years)
} } } that still do yeoman's service day after day and year after year. If
} } } your
} } } SEM has the capabilities you need, hang on to it.
} } }
} } } I'm sorry that I don't even have any suggestions on what to do
} } } when the
} } } vendor runs out of DX4 spares. Perhaps get a boat so that you can
} } } moorit
} } } to a very expensive anchor.
} } }
} } } If the manufacturers would open their source code, someone who knows
} } } programming might be able to update the software to a newer PC as
} } long
} } } as
} } } the connection is a network connection rather than proprietary driver
} } } boards
} } } for an obsolete bus.
} } }
} } } It is up to the users to demand a longer view towards these 6 (and 7)
} } } figure
} } } investments.
} } }
} } } Ken Converse
} } } owner
} } }
} } } QUALITY IMAGES
} } } Servicing Scanning Electron Microscopes
} } } Since 1981
} } } 474 So. Bridgton Rd.
} } } Bridgton, ME 04009
} } } 207-647-4348
} } } Fax 207-647-2688
} } } kenconverse-at-qualityimages.biz
} } } qualityimages.biz
} } }
} } } DISCLAIMER: Quality Images provides both per call and full service
} } } contracts on non-PC-controlled SEMs in the northeastern United
} } States.
} } }
} } }
} } } -----Original Message-----
} } } X-from: Nicola.Weston-at-nottingham.ac.uk
} } } [mailto:Nicola.Weston-at-nottingham.ac.uk]
} } }
} } } Hi
} } } I think I should clarify that we have a service contract & good
} } } supportfrom FEI. My main concern is the integrated DX4 pc, which
} } } has been
} } } upgraded as far as possible. So far we have been able to buy parts
} } } fromFEI but I am worried about future sourcing of spares for our
} } } system as
} } } parts are no longer made.
} } } It's good to know about second hand supplies from elsewhere though,
} } } thanks for all your replies.
} } }
} } } Regards
} } } Nikki
} } }
} } }
} } }
} } } Nikki
} } }
} } } Are you saying that FEI will not sell you the parts?
} } }
} } } regards,
} } }
} } } Jim
} } }
} } }
} } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date:
} } } Mon, 2
} } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu }
} } } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to:
} } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy
} } } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy]
} } } ESEM users }
} } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } } } X-lewp: MicroscopyListSpam NAGS
} } } }
} } } -------------------------------------------------------------------
} } } -----
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html }
} } } -------------------------------------------------------------------
} } } -----
} } } }
} } } } Hello
} } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are
} } } }
} } } beginning to encounter problems with sourcing spare parts. Just }
} } } wondering if anyone else out there is running an xl30 esem and if
} } } so are
} } } } you having similar problems now they are no longer made,or does
} } } everyone } have a Quanta these days?
} } } }
} } } } Thanks
} } } } Nikki Weston
} } } } School M3
} } } } University of Nottingham
} } }
} } } This message has been checked for viruses but the contents of an
} } } attachment
} } } may still contain software viruses, which could damage your computer
} } } system:
} } } you are advised to perform your own checks. Email communications with
} } } the
} } } University of Nottingham may be monitored as permitted by UK
} } } legislation.
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================31, 36 -- From
} } } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009
} } } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub-
} } } 4.iastate.edu [129.186.140.14])
} } } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } } ESMTP id n24FSFIW022376
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:15 -0600
} } } 31, 36 -- Received: from devirus-10.iastate.edu (devirus-
} } } 10.iastate.edu [129.186.1.47])
} } } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10)
} } } with SMTP id n24FSETv025573
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:14 -0600
} } } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80])
} } } by devirus-10.iastate.edu with smtp
} } } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a;
} } } 31, 36 -- Wed, 04 Mar 2009 09:10:28 -0600
} } } 31, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
} } } [129.186.23.85])31, 36 -- by despam-10.iastate.edu
} } } (8.14.2/8.12.10) with ESMTP id n24FSD1H004703
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:13 -0600
} } } 31, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
} } } owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } } 31, 36 -- Wed, 4 Mar 2009 09:28:14 -0600
} } } 31, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } } 31, 36 -- Content-class: urn:content-classes:message
} } } 31, 36 -- MIME-Version: 1.0
} } } 31, 36 -- Content-Type: text/plain;
} } } 31, 36 -- charset="us-ascii"
} } } 31, 36 -- Subject: RE: [Microscopy] RE: Esem users
} } } 31, 36 -- Date: Wed, 4 Mar 2009 09:28:35 -0600
} } } 31, 36 -- Message-ID:
} } } {16A330AC32056A40B32842EC4BB8D72703B2A1D0-at-maire.eng.iastate.edu} 31,
} } 36 -- In-Reply-To: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } } 31, 36 -- X-MS-Has-Attach:
} } } 31, 36 -- X-MS-TNEF-Correlator:
} } } 31, 36 -- Thread-Topic: [Microscopy] RE: Esem users
} } } 31, 36 -- Thread-Index: Acmc21oidxgM5IUFT72HTN2CRnlTRgAATOqQ
} } } 31, 36 -- References: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } } 31, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} } } 31, 36 -- To: {Microscopy-at-microscopy.com}
} } } 31, 36 -- X-OriginalArrivalTime: 04 Mar 2009 15:28:14.0531 (UTC)
} } } FILETIME=[D5CAC130:01C99CDD]31, 36 -- X-PMX-Version: 5.5.2.365749,
} } } Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.151931
} } } 31, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
} } } Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0,
} } } BODY_SIZE_7000_LESS 0, OEM_SOFTWARE_X1 0, __BOUNCE_CHALLENGE_SUBJ
} } } 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0,
} } } __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0,
} } } __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0,
} } } __PHISH_SPEAR_REASONS 0, __SANE_MSGID 0'
} } } 31, 36 -- Content-Transfer-Encoding: 8bit
} } } 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } } ns.microscopy.com id n24FSFIW022376
} } } ==============================End of -
} } } Headers==============================
} }
} } ==============================Original Headers==============================
} } 11, 35 -- From malcolm.haswell-at-sunderland.ac.uk Wed Mar 4 13:25:31 2009
} } 11, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk [157.228.29.83])
} } 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24JPUZh001937
} } 11, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:25:31 -0600
} } 11, 35 -- Received: (qmail 4727 invoked from network); 4 Mar 2009 17:01:24 -0000
} } 11, 35 -- Received: from localhost (127.0.0.1)
} } 11, 35 -- by max1.sunderland.ac.uk with SMTP; 4 Mar 2009 17:01:24 -0000
} } 11, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1])
} } 11, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024)
} } 11, 35 -- with SMTP id 03643-04 for {Microscopy-at-microscopy.com} ;
} } 11, 35 -- Wed, 4 Mar 2009 17:01:13 +0000 (GMT)
} } 11, 35 -- Received: (qmail 4643 invoked by uid 607); 4 Mar 2009 17:01:13 -0000
} } 11, 35 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117)
} } 11, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 04 Mar 2009 17:01:13 +0000
} } 11, 35 -- Received: from sunderland.ac.uk (localhost [127.0.0.1])
} } 11, 35 -- by hermes.sunderland.ac.uk
} } 11, 35 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004))
} } 11, 35 -- with ESMTP id {0KFZ0039VRA25L-at-hermes.sunderland.ac.uk} for
} } 11, 35 -- Microscopy-at-microscopy.com; Wed, 04 Mar 2009 17:01:15 +0000 (GMT)
} } 11, 35 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed,
} } 11, 35 -- 04 Mar 2009 17:01:14 +0000 (GMT)
} } 11, 35 -- Date: Wed, 04 Mar 2009 17:01:14 +0000 (GMT)
} } 11, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
} } 11, 35 -- Subject: Re: [Microscopy] Esem users
} } 11, 35 -- To: wesaia-at-iastate.edu, Microscopy MSA {Microscopy-at-microscopy.com}
} } 11, 35 -- Message-id: {add89b3988.b3988add89-at-sunderland.ac.uk}
} } 11, 35 -- MIME-version: 1.0
} } 11, 35 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004)
} } 11, 35 -- Content-type: text/plain; charset=us-ascii
} } 11, 35 -- Content-language: en
} } 11, 35 -- Content-transfer-encoding: 7BIT
} } 11, 35 -- Content-disposition: inline
} } 11, 35 -- X-Accept-Language: en
} } 11, 35 -- Priority: normal
} } 11, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk
} } ==============================End of - Headers==============================


==============================Original Headers==============================
2, 26 -- From maryflet-at-interchange.ubc.ca Wed Mar 4 14:40:14 2009
2, 26 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca [137.82.45.7])
2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24KeDSM007886
2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:40:14 -0600
2, 26 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70])
2, 26 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id 9033C1B262
2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:40:12 -0800 (PST)
2, 26 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
2, 26 -- by smtp.interchange.ubc.ca
2, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
2, 26 -- with ESMTP id {0KG0003BW1BVKT-at-smtp.interchange.ubc.ca} for
2, 26 -- microscopy-at-microscopy.com; Wed, 04 Mar 2009 12:40:12 -0800 (PST)
2, 26 -- Date: Wed, 04 Mar 2009 12:40:11 -0800 (PST)
2, 26 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
2, 26 -- Subject: Fwd: Re: [Microscopy] Re: Esem users
2, 26 -- To: microscopy-at-microscopy.com
2, 26 -- Message-id: {15129506.7991236199211456.JavaMail.myubc2-at-handel.my.ubc.ca}
2, 26 -- MIME-version: 1.0
2, 26 -- X-Mailer: uPortal WEB email client 3.0
2, 26 -- Content-type: text/plain; charset=us-ascii
2, 26 -- Content-transfer-encoding: 7bit
2, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.202241
2, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
2, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_10000_PLUS 0, OEM_SOFTWARE_X1 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0, __PHISH_SPEAR_REASONS 0, __PHISH_SPEAR_STRUCTURE_1 0, __SANE_MSGID 0
2, 26 -- X-Spam-Level:
2, 26 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Wed, 4 Mar 2009 15:27:24 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

As it often happens there is truth to both sides of the story. Inherently
digital operations, like image processing, are done and best controlled by
computers; routine and well established procedures are more efficient with
automated macros and pre-saved settings, also computer is less likely to
forget about safety checks for stage travel limits, etc... (of cause after
all bugs were worked out).

But it is probably easier to develop "feel" for inherently "manual"
operations, like alignments or stigmation for example, by turning knobs
(even though automated focus/stig works most of the times on most of the
tools). It is also likely that "non-standard" research applications may call
for settings and combinations of adjustments unforeseen by GUI programmer
(or the system engineer who wrote SOW for him/her).

Digital image processing is a big part of the tool capabilities and requires
computer, but keeping in mind that equipment manufacturers are first and
foremost money-making organizations becomes easier to see another (maybe
main?) factor behind heavier reliance on GUI software and elimination of
knob controls in some instruments - manufacturing costs of GUI are zero!
Computer is part of the system already, and after the development is done
and its expenses written off - there is no extra hardware to build into the
machine, making BOM and manufacturing books look better. Modifications of
GUI are also far less expensive then modifying hard-wired knob panel.

Because of hardware costs factor, future instruments will be even more
computer-based, but IMHO (which did not change in last 10 years) instruments
intended for serious work should have both GUI and the knob controls
available. Almost impossible to foresee all the applications where research
tool will be used, and limiting controls to only the GUI has danger of
locking operator in the inherently limited box of pre-defined capabilities,
which may actually be good in some situations but very frustrating in
others. There are ways to keep both "knob turning" and "mouse clicking"
users happy and instrument controls both convenient and flexible, just takes
some thinking and occasional look "out of the box".


Just my 2C, not that anybody cares :)

Cheers,
Valery Ray

============================
www.partbeamsystech.com
PBS&T, MEO Engineering Co, Inc.
290 Broadway St., Suite 298
Methuen, MA 01844
Phone: (978) 296-5063


-----Original Message-----
X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
Sent: Wednesday, March 04, 2009 2:06 PM
To: vray-at-partbeamsystech.com

Hi all,
Just an alternative perspective.

Maybe I'm just a computer geek or maybe it's the FEI interface (which
I find it simple, easy and intuitive), but even with the dedicated
knob set (which I insisted on when we bought the instrument, having
been an "analog knob feel" junkie myself), I find I only ever use the
big mag knob to zoom in or out.

The mouse based focus and stig I find effortless to use and very
responsive (it's right button click and drag (in X) to focus and
shift right click and drag (in X and Y) to stigmate).

I am completely won over by the FEI Quanta ESEM computer control much
to my surprise.
john



At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote:
} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009
8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu
[128.223.142.40])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n24J40vi031984
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:00
-0600
8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu
[128.223.10.46])
8, 21 -- (authenticated bits=0)
8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id
n24J3x0h006753
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NOT)
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:04:00
-0800
8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu}
8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800
8, 21 -- To: microscopy-at-microscopy.com
8, 21 -- From: John Donovan {donovan-at-uoregon.edu}
8, 21 -- Subject: Re: [Microscopy] Esem users
8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com}
8, 21 -- Mime-Version: 1.0
8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on
mserv1
8, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================


==============================Original Headers==============================
24, 25 -- From vray-at-partbeamsystech.com Wed Mar 4 15:27:24 2009
24, 25 -- Received: from smtp107.biz.mail.re2.yahoo.com (smtp107.biz.mail.re2.yahoo.com [206.190.52.176])
24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24LRNj9022890
24, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:27:23 -0600
24, 25 -- Message-Id: {200903042127.n24LRNj9022890-at-ns.microscopy.com}
24, 25 -- Received: (qmail 85032 invoked from network); 4 Mar 2009 21:27:23 -0000
24, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.106.16 with login)
24, 25 -- by smtp107.biz.mail.re2.yahoo.com with SMTP; 4 Mar 2009 21:27:22 -0000
24, 25 -- X-YMail-OSG: PFBAEh8VM1k1q90G9L5.8EGg0rigdp1kQIk4DQeV0h5I4TZowkoiFr7L2QIB3ZfWznaI6mBtpodXD54RP4pLzeZTslWP7vxxGIlz4Sn0TsSRm9ylqYY7Rjv8DJPqtRavepH8cajRyIfUnv.psQLty1FPilrzi9DzsV1Qvxl7LXP2utl0zMcdByqK7UyW3vdSnXqBZxvIigjZt6kOeA_kVylnd1.mcMtb
24, 25 -- X-Yahoo-Newman-Property: ymail-3
24, 25 -- Reply-To: {vray-at-partbeamsystech.com}
24, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com}
24, 25 -- To: {donovan-at-uoregon.edu}
24, 25 -- Cc: {microscopy-at-microscopy.com}
24, 25 -- Subject: RE: [Microscopy] Re: Esem users
24, 25 -- Date: Wed, 4 Mar 2009 16:30:16 -0500
24, 25 -- Organization: PBST / MEO Engineering
24, 25 -- MIME-Version: 1.0
24, 25 -- Content-Type: text/plain;
24, 25 -- charset="us-ascii"
24, 25 -- Content-Transfer-Encoding: 7bit
24, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
24, 25 -- Thread-Index: Acmc/DHrG15s9TNDTR2svDzY8R2yhAAB7NvQ
24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 25 -- In-Reply-To: {200903041905.n24J5VPs002606-at-ns.microscopy.com}
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 4 Mar 2009 15:35:00 -0600
Subject: [Microscopy] Fwd: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary,
You're right that the electromechanical (and optomechanical) components are
more expensive, but they can at least be replaced (and usually easily
trouble-shot) and sometimes repaired on site. Most last for many years
without any problems (and they don't require upgrades).

When it costs $25-30,000 to replace a $2000 computer with a $1000 computer
that is already out of date when it's installed would give me serious pause.
That is the price range of upgrades that I've heard about. I don't know
what yours cost.

I have resigned myself (sadly) to the fact that the computers I use are mere
commodities that are often cheaper to replace than to try and repair. I
have some difficulty coming to the same conclusion on equipment that costs
as much as an SEM, uses the resources that are used in making an SEM, and
whose main components are built to last decades. It just doesn't make sense
economically, environmentally or socially to just chuck this stuff out when
the basic PC morphs so fast and (previous comments not withstanding) can no
longer run the equipment due to previously discussed design parameters (that
should be changed, as discussed in a thread a few months ago).

Maybe Hitachi is paying attention and that is why the upgrade went so
smoothly, although you haven't said what that $1000 computer cost.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: maryflet-at-interchange.ubc.ca [mailto:maryflet-at-interchange.ubc.ca]
Sent: Wednesday, March 04, 2009 3:42 PM
To: kenconverse-at-qualityimages.biz

} Dear Malcolm,
} I recently upgraded my Hitachi S3000N computer and it was not a problem,
just cost money and required a service call, because there are things to
change inside the SEM, as well as the computer and monitor. The computer has
to be configured in Japan. The new system boots very fast, I got new
software that is a bit nicer and I won't be obselete for another few years.
} On this thread, one of the reasons that the SEMs are all run on consumer
computers, besides the fact that customers demanded it, is that it is way
cheaper. Turn knobs, pots and other discrete components are expensive, now,
compared to software, which gets cheaper the more systems you install.
} My two cents worth.
} Regards,
} Mary Fletcher
}
}
}
} -----Original Message-----
}
} } Date: Wed Mar 04 11:31:51 PST 2009
} } From: malcolm.haswell-at-sunderland.ac.uk
} } Subject: [Microscopy] Re: Esem users
} } To: maryflet-at-interchange.ubc.ca
} }
} }
} }
} }
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } Warren
} }
} } this does raise one or two interesting points about computers in
} } microscopy.
} }
} } We fairly easily upgraded the operating system on our Link EDS system
} } several years ago but had a lot of problems even discussing the use of
} } new printer drivers for our Hitachi S3000N.
} }
} } I get the impression that microscope control systems and imaging are
} } so heavily integrated into their computers that a simple upgrade is
} } not possible because of the complexity of hardware, bespoke drivers,
} } special software and the operating system and peripherals. Maybe this
} } will change with 2nd and 3rd generation systems.
} }
} } Analytical EDS systems which have been computerized for years seem a
} } bit easier because they have less integrated controls, no active
} } imaging and software packages seem a bit more portable.
} }
} } Maybe as well as looking out for sales of microscopy spares it might
} } be useful to keep a couple of legacy computers for the technologies
} } that won't migrate onto newer computers.
} }
} } Malcolm
} }
} } Malcolm Haswell
} } Electron Microscope Unit
} } Faculty of Applied Sciences
} } University of Sunderland
} } SUNDERLAND
} } SR1 3SD
} } UK
} }
} } email: malcolm.haswell-at-sunderland.ac.uk
} }
} }
} } ----- Original Message -----
} } X-from: wesaia-at-iastate.edu
} } Date: Wednesday, March 4, 2009 3:33 pm
} } Subject: [Microscopy] Esem users
} }
} } }
} } }
} } }
} } } -------------------------------------------------------------------
} } } ---------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserverOn-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } ----------------------------------------------------------------
} } }
} } } Nikki, how embedded is that PC? How peculiar is it?
} } }
} } } I would think if you have a decent computer person around, you could
} } } upgrade on your own. There could be timing issues and you might need
} } } support for an ISA slot or two, but you should be able to do a
} } } lot. I
} } } would not count on the manufacturer for help.
} } }
} } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3
} } } to a
} } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us
} } along
} } } the way and would not guarantee that our upgrade would work. They had
} } } not tested our exact hardware and could not guarantee success. But
} } } neither did they promise failure. The upgrade worked fine. We do know
} } } that the software breaks when we upgrade to Windows XP, so we are
} } } maxedout with the OS if not the hardware. Fortunately, the
} } } hardware is
} } } current and stable and snappy as it is.
} } }
} } } If you do need a replacement DX4, they are still out there on the
} } } market. I found several links to ones. I might even have one
} } gathering
} } } dust at home. I know I have several older, working PCs with ISA
} } } slots.
} } }
} } } Warren S.
} } }
} } }
} } } -----Original Message-----
} } } X-from: kenconverse-at-qualityimages.biz
} } } [mailto:kenconverse-at-qualityimages.biz]
} } } Sent: Wednesday, March 04, 2009 9:10 AM
} } } To: wesaia-at-iastate.edu
} } } Subject: [Microscopy] RE: Esem users
} } }
} } } -------------------------------------------------------------------
} } } -----
} } }
} } } Hi Nikki,
} } } And there you have it: the number one reason not to abandon a
} } } pre-PC-controlled SEM - the PC.
} } }
} } } I'm still servicing 30+ year old SEMs (at least one approaching 40
} } } years)
} } } that still do yeoman's service day after day and year after year. If
} } } your
} } } SEM has the capabilities you need, hang on to it.
} } }
} } } I'm sorry that I don't even have any suggestions on what to do
} } } when the
} } } vendor runs out of DX4 spares. Perhaps get a boat so that you can
} } } moorit
} } } to a very expensive anchor.
} } }
} } } If the manufacturers would open their source code, someone who knows
} } } programming might be able to update the software to a newer PC as
} } long
} } } as
} } } the connection is a network connection rather than proprietary driver
} } } boards
} } } for an obsolete bus.
} } }
} } } It is up to the users to demand a longer view towards these 6 (and 7)
} } } figure
} } } investments.
} } }
} } } Ken Converse
} } } owner
} } }
} } } QUALITY IMAGES
} } } Servicing Scanning Electron Microscopes
} } } Since 1981
} } } 474 So. Bridgton Rd.
} } } Bridgton, ME 04009
} } } 207-647-4348
} } } Fax 207-647-2688
} } } kenconverse-at-qualityimages.biz
} } } qualityimages.biz
} } }
} } } DISCLAIMER: Quality Images provides both per call and full service
} } } contracts on non-PC-controlled SEMs in the northeastern United
} } States.
} } }
} } }
} } } -----Original Message-----
} } } X-from: Nicola.Weston-at-nottingham.ac.uk
} } } [mailto:Nicola.Weston-at-nottingham.ac.uk]
} } }
} } } Hi
} } } I think I should clarify that we have a service contract & good
} } } supportfrom FEI. My main concern is the integrated DX4 pc, which
} } } has been
} } } upgraded as far as possible. So far we have been able to buy parts
} } } fromFEI but I am worried about future sourcing of spares for our
} } } system as
} } } parts are no longer made.
} } } It's good to know about second hand supplies from elsewhere though,
} } } thanks for all your replies.
} } }
} } } Regards
} } } Nikki
} } }
} } }
} } }
} } } Nikki
} } }
} } } Are you saying that FEI will not sell you the parts?
} } }
} } } regards,
} } }
} } } Jim
} } }
} } }
} } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date:
} } } Mon, 2
} } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu }
} } } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to:
} } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy
} } } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy]
} } } ESEM users }
} } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } } } X-lewp: MicroscopyListSpam NAGS
} } } }
} } } -------------------------------------------------------------------
} } } -----
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html }
} } } -------------------------------------------------------------------
} } } -----
} } } }
} } } } Hello
} } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are
} } } }
} } } beginning to encounter problems with sourcing spare parts. Just }
} } } wondering if anyone else out there is running an xl30 esem and if
} } } so are
} } } } you having similar problems now they are no longer made,or does
} } } everyone } have a Quanta these days?
} } } }
} } } } Thanks
} } } } Nikki Weston
} } } } School M3
} } } } University of Nottingham
} } }
} } } This message has been checked for viruses but the contents of an
} } } attachment
} } } may still contain software viruses, which could damage your computer
} } } system:
} } } you are advised to perform your own checks. Email communications with
} } } the
} } } University of Nottingham may be monitored as permitted by UK
} } } legislation.
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================31, 36 -- From
} } } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009
} } } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub-
} } } 4.iastate.edu [129.186.140.14])
} } } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } } ESMTP id n24FSFIW022376
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:15 -0600
} } } 31, 36 -- Received: from devirus-10.iastate.edu (devirus-
} } } 10.iastate.edu [129.186.1.47])
} } } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10)
} } } with SMTP id n24FSETv025573
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:14 -0600
} } } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80])
} } } by devirus-10.iastate.edu with smtp
} } } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a;
} } } 31, 36 -- Wed, 04 Mar 2009 09:10:28 -0600
} } } 31, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
} } } [129.186.23.85])31, 36 -- by despam-10.iastate.edu
} } } (8.14.2/8.12.10) with ESMTP id n24FSD1H004703
} } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009
} } } 09:28:13 -0600
} } } 31, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
} } } owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } } 31, 36 -- Wed, 4 Mar 2009 09:28:14 -0600
} } } 31, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } } 31, 36 -- Content-class: urn:content-classes:message
} } } 31, 36 -- MIME-Version: 1.0
} } } 31, 36 -- Content-Type: text/plain;
} } } 31, 36 -- charset="us-ascii"
} } } 31, 36 -- Subject: RE: [Microscopy] RE: Esem users
} } } 31, 36 -- Date: Wed, 4 Mar 2009 09:28:35 -0600
} } } 31, 36 -- Message-ID:
} } } {16A330AC32056A40B32842EC4BB8D72703B2A1D0-at-maire.eng.iastate.edu} 31,
} } 36 -- In-Reply-To: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } } 31, 36 -- X-MS-Has-Attach:
} } } 31, 36 -- X-MS-TNEF-Correlator:
} } } 31, 36 -- Thread-Topic: [Microscopy] RE: Esem users
} } } 31, 36 -- Thread-Index: Acmc21oidxgM5IUFT72HTN2CRnlTRgAATOqQ
} } } 31, 36 -- References: {200903041510.n24FAPnO009143-at-ns.microscopy.com}
} } } 31, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} } } 31, 36 -- To: {Microscopy-at-microscopy.com}
} } } 31, 36 -- X-OriginalArrivalTime: 04 Mar 2009 15:28:14.0531 (UTC)
} } } FILETIME=[D5CAC130:01C99CDD]31, 36 -- X-PMX-Version: 5.5.2.365749,
} } } Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.151931
} } } 31, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
} } } Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0,
} } } BODY_SIZE_7000_LESS 0, OEM_SOFTWARE_X1 0, __BOUNCE_CHALLENGE_SUBJ
} } } 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0,
} } } __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0,
} } } __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0,
} } } __PHISH_SPEAR_REASONS 0, __SANE_MSGID 0'
} } } 31, 36 -- Content-Transfer-Encoding: 8bit
} } } 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } } ns.microscopy.com id n24FSFIW022376
} } } ==============================End of -
} } } Headers==============================
} }
} } ==============================Original
Headers==============================
} } 11, 35 -- From malcolm.haswell-at-sunderland.ac.uk Wed Mar 4 13:25:31 2009
} } 11, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk
[157.228.29.83])
} } 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n24JPUZh001937
} } 11, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:25:31
-0600
} } 11, 35 -- Received: (qmail 4727 invoked from network); 4 Mar 2009
17:01:24 -0000
} } 11, 35 -- Received: from localhost (127.0.0.1)
} } 11, 35 -- by max1.sunderland.ac.uk with SMTP; 4 Mar 2009 17:01:24
-0000
} } 11, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1])
} } 11, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1])
(amavisd-new, port 10024)
} } 11, 35 -- with SMTP id 03643-04 for {Microscopy-at-microscopy.com} ;
} } 11, 35 -- Wed, 4 Mar 2009 17:01:13 +0000 (GMT)
} } 11, 35 -- Received: (qmail 4643 invoked by uid 607); 4 Mar 2009 17:01:13
-0000
} } 11, 35 -- Received: from unknown (HELO hermes.sunderland.ac.uk)
(157.228.37.117)
} } 11, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed,
04 Mar 2009 17:01:13 +0000
} } 11, 35 -- Received: from sunderland.ac.uk (localhost [127.0.0.1])
} } 11, 35 -- by hermes.sunderland.ac.uk
} } 11, 35 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004))
} } 11, 35 -- with ESMTP id {0KFZ0039VRA25L-at-hermes.sunderland.ac.uk} for
} } 11, 35 -- Microscopy-at-microscopy.com; Wed, 04 Mar 2009 17:01:15 +0000
(GMT)
} } 11, 35 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk
(mshttpd); Wed,
} } 11, 35 -- 04 Mar 2009 17:01:14 +0000 (GMT)
} } 11, 35 -- Date: Wed, 04 Mar 2009 17:01:14 +0000 (GMT)
} } 11, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
} } 11, 35 -- Subject: Re: [Microscopy] Esem users
} } 11, 35 -- To: wesaia-at-iastate.edu, Microscopy MSA
{Microscopy-at-microscopy.com}
} } 11, 35 -- Message-id: {add89b3988.b3988add89-at-sunderland.ac.uk}
} } 11, 35 -- MIME-version: 1.0
} } 11, 35 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14
2004)
} } 11, 35 -- Content-type: text/plain; charset=us-ascii
} } 11, 35 -- Content-language: en
} } 11, 35 -- Content-transfer-encoding: 7BIT
} } 11, 35 -- Content-disposition: inline
} } 11, 35 -- X-Accept-Language: en
} } 11, 35 -- Priority: normal
} } 11, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk
} } ==============================End of -
Headers==============================


==============================Original Headers==============================
2, 26 -- From maryflet-at-interchange.ubc.ca Wed Mar 4 14:40:14 2009
2, 26 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca
[137.82.45.7])
2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n24KeDSM007886
2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:40:14
-0600
2, 26 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca
[142.103.145.70])
2, 26 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id 9033C1B262
2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:40:12
-0800 (PST)
2, 26 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
2, 26 -- by smtp.interchange.ubc.ca
2, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
2, 26 -- with ESMTP id {0KG0003BW1BVKT-at-smtp.interchange.ubc.ca} for
2, 26 -- microscopy-at-microscopy.com; Wed, 04 Mar 2009 12:40:12 -0800 (PST)
2, 26 -- Date: Wed, 04 Mar 2009 12:40:11 -0800 (PST)
2, 26 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
2, 26 -- Subject: Fwd: Re: [Microscopy] Re: Esem users
2, 26 -- To: microscopy-at-microscopy.com
2, 26 -- Message-id:
{15129506.7991236199211456.JavaMail.myubc2-at-handel.my.ubc.ca}
2, 26 -- MIME-version: 1.0
2, 26 -- X-Mailer: uPortal WEB email client 3.0
2, 26 -- Content-type: text/plain; charset=us-ascii
2, 26 -- Content-transfer-encoding: 7bit
2, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine:
2.6.1.350677, Antispam-Data: 2009.3.4.202241
2, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
2, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05,
BODY_SIZE_10000_PLUS 0, OEM_SOFTWARE_X1 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER
0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0,
__CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0,
__KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0,
__MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0,
__PHISH_SPEAR_REASONS 0, __PHISH_SPEAR_STRUCTURE_1 0, __SANE_MSGID 0
2, 26 -- X-Spam-Level:
2, 26 -- X-Spam-Flag: No
==============================End of - Headers==============================




==============================Original Headers==============================
17, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 15:35:00 2009
17, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24LZ0Fc004165
17, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:35:00 -0600
17, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
17, 26 -- with ESMTP
17, 26 -- id {20090304213459.VUDA5856.cdptpa-omta04.mail.rr.com-at-Ken} ;
17, 26 -- Wed, 4 Mar 2009 21:34:59 +0000
17, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
17, 26 -- To: {maryflet-at-interchange.ubc.ca} ,
17, 26 -- "MSA Listserver" {microscopy-at-microscopy.com}
17, 26 -- Subject: RE: [Microscopy] Fwd: Esem users
17, 26 -- Date: Wed, 4 Mar 2009 16:34:53 -0500
17, 26 -- Message-ID: {A57062B199364760B43D652B5D534AAF-at-Ken}
17, 26 -- MIME-Version: 1.0
17, 26 -- Content-Type: text/plain;
17, 26 -- charset="us-ascii"
17, 26 -- X-Priority: 3 (Normal)
17, 26 -- X-MSMail-Priority: Normal
17, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
17, 26 -- Importance: Normal
17, 26 -- Thread-Index: AcmdCbq9IMl2+RlVTbipBGq7mXgWCQABGAdQ
17, 26 -- In-Reply-To: {200903042042.n24KgPHu011869-at-ns.microscopy.com}
17, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
17, 26 -- Content-Transfer-Encoding: 8bit
17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24LZ0Fc004165
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Wed, 4 Mar 2009 15:53:40 -0600
Subject: [Microscopy] Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

May as well add another 2c (US 1.3c)....

If you're in a dry climate like Canberra (unlike the wet murk of AKL),
sadly, your 2-3-decade old JEOL boards have started to crack and it takes
quite a while to find the dry join that needs re-soldering on those big
boards - and the admin rulers of the roost will not fork out for maintenance
any more. And the most well-used knobs and dials have worn out or are
wearing out and some are hard to replace. A great old instrument brought
down by incremental decline.

But in our new SEM (symbiotically linked to computer) two of the main
motherboards spat the dummy within a month(!) and of course, the instrument
was unusable.... Otherwise, a nice instrument.....

cheers,
Rosemary


Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 5/03/09 7:27 AM, "r.sims-at-auckland.ac.nz" {r.sims-at-auckland.ac.nz} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Hi
}
} This is an interesting discussion. I'm pleased to see that I'm not the only
} person with deep-
} seated reservations about computerised instrumentation. It costs a fortune to
} get anything
} professionally serviced down here as real expertise is often only to be found
} in Australia, and
} by the time accomodation, travel cost, and travel time is added, a one-day
} visit can cost
} thousands of our shrinking dollars.
}
} Australia and New Zealand are not, in fact, all that close together!
}
} My 1987 JEOL 840 has a totally mysterious and enigmatic CPU embedded deep
} within it,
} being, I guess the early days of microcomputerisation, the documentation has
} only an
} occasional reference to it.
}
} I live in fear of it malfunctioning but am comforted somewhat by the amazing
} reliability of the
} JEOL electronics.
}
} If it dies or even gets sick, is that the end for the instrument?
}
} Anybody been there with an 840?
}
} cheers
} Ritchie Sims
}
}
}
}
} --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9 3737435
} Department of Geology email : r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
} ==============================Original Headers==============================
} 14, 30 -- From r.sims-at-auckland.ac.nz Wed Mar 4 14:21:54 2009
} 14, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz
} [130.216.12.34])
} 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n24KLrs3026200
} 14, 30 -- for {microscopy-at-Microscopy.Com} ; Wed, 4 Mar 2009 14:21:53 -0600
} 14, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3EA991AA7A
} 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:52 +1300
} (NZDT)
} 14, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
} 14, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
} 14, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new,
} port 10024)
} 14, 30 -- with ESMTP id VWtYifBHddk0 for {microscopy-at-Microscopy.Com} ;
} 14, 30 -- Thu, 5 Mar 2009 09:21:52 +1300 (NZDT)
} 14, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz
} [130.216.59.18])
} 14, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
} 14, 30 -- (No client certificate requested)
} 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1F5AA1AA70
} 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:51 +1300
} (NZDT)
} 14, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} 14, 30 -- To: microscopy-at-Microscopy.Com
} 14, 30 -- Date: Thu, 05 Mar 2009 09:24:00 +1300
} 14, 30 -- MIME-Version: 1.0
} 14, 30 -- Subject: Re: [Microscopy] Esem users
} 14, 30 -- Message-ID: {49AF9A30.12078.415D66-at-r.sims.auckland.ac.nz}
} 14, 30 -- Priority: normal
} 14, 30 -- In-reply-to: {200903042006.n24K6SAt008123-at-ns.microscopy.com}
} 14, 30 -- References: {200903042006.n24K6SAt008123-at-ns.microscopy.com}
} 14, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
} 14, 30 -- Content-type: text/plain; charset=US-ASCII
} 14, 30 -- Content-transfer-encoding: 7BIT
} 14, 30 -- Content-description: Mail message body
} ==============================End of - Headers==============================



==============================Original Headers==============================
12, 43 -- From prvs=3076719c4=Rosemary.White-at-csiro.au Wed Mar 4 15:53:39 2009
12, 43 -- Received: from vic-MTAout4.csiro.au (vic-MTAout4.csiro.au [150.229.64.41])
12, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24LrbG8018129
12, 43 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:53:38 -0600
12, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
12, 43 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
12, 43 -- s=email; t=1236203617; x=1267739617;
12, 43 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
12, 43 -- mime-version:content-transfer-encoding:content-id:
12, 43 -- content-description:resent-date:resent-from:resent-sender:
12, 43 -- resent-to:resent-cc:resent-message-id:in-reply-to:
12, 43 -- references:list-id:list-help:list-unsubscribe:
12, 43 -- list-subscribe:list-post:list-owner:list-archive;
12, 43 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
12, 43 -- |Subject:=20Re:=20[Microscopy]=20Re:=20=20Esem=20users
12, 43 -- |Date:=20Thu,=205=20Mar=202009=2008:53:34=20+1100
12, 43 -- |Message-ID:=20 {C5D5438E.3E49%rosemary.white-at-csiro.au}
12, 43 -- |To:=20 {microscopy-at-Microscopy.Com} |MIME-Version:=201.0
12, 43 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200903
12, 43 -- 042027.n24KR5fu002922-at-ns.microscopy.com} ;
12, 43 -- bh=B6wWfByzasFR+yxFvpewRdce6dtsHXwJ0CmyjNdkO+4=;
12, 43 -- b=txp4iD+FAVoBT1vquuoIXyihuRSn3kAu6PflxTA1+vICjROcxHG0sejm
12, 43 -- Bn+oRpqyfwBIp3NA43BlVn7L9eoXWCflRLgiddYzhPNJgRsLXtjjtA6av
12, 43 -- mhVLfEkzUs1HV+m;
12, 43 -- X-IronPort-AV: E=Sophos;i="4.38,303,1233493200";
12, 43 -- d="scan'208";a="10659009"
12, 43 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
12, 43 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 05 Mar 2009 08:53:34 +1100
12, 43 -- Received: from [152.83.167.123] (152.83.167.123) by
12, 43 -- EXNSW-HTCA01.nexus.csiro.au (130.155.117.126) with Microsoft SMTP Server id
12, 43 -- 8.1.340.0; Thu, 5 Mar 2009 08:53:35 +1100
12, 43 -- User-Agent: Microsoft-Entourage/12.10.0.080409
12, 43 -- Date: Thu, 5 Mar 2009 08:53:34 +1100
12, 43 -- Subject: Re: [Microscopy] Re: Esem users
12, 43 -- From: Rosemary White {rosemary.white-at-csiro.au}
12, 43 -- To: {microscopy-at-microscopy.com}
12, 43 -- Message-ID: {C5D5438E.3E49%rosemary.white-at-csiro.au}
12, 43 -- Thread-Topic: [Microscopy] Re: Esem users
12, 43 -- Thread-Index: AcmdB5kvb9ewpCjKTyuL6hL6SEw+4gADBDhC
12, 43 -- In-Reply-To: {200903042027.n24KR5fu002922-at-ns.microscopy.com}
12, 43 -- MIME-Version: 1.0
12, 43 -- Content-Type: text/plain; charset="US-ASCII"
12, 43 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: lbustillos-at-emsl.com
Date: Wed, 4 Mar 2009 19:25:50 -0600
Subject: [Microscopy] viaWWW: Objective for Dispersion Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lbustillos-at-emsl.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lbustillos-at-emsl.com
Name: Louie Bustillos

Organization: EMSL Analytical, Inc.

Title-Subject: [Filtered] Objective for Dispersion Staining

Question: Hello,

I would like to know if anybody knows who can make a centerable
central stop in a 10X objective in order to do dispersion staining?
I have found objectives that have a central stop but they are
permanently mounted. This is not a problem if the central stop is
perfectly centered but most of the ones that I have seen are not
perfectly centered and this causes problems with trying to see
dispersion colors.


Thank you for your time.

Louie

Login Host: 70.196.35.154
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:25:50 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n251PnpD004686
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:25:49 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c5d4da89a8ad-at-[206.69.208.22]}
10, 11 -- Date: Wed, 4 Mar 2009 19:25:48 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: lbustillos-at-emsl.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Objective for Dispersion Staining
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: a.l.bleloch-at-liv.ac.uk
Date: Wed, 4 Mar 2009 19:26:18 -0600
Subject: [Microscopy] viaWWW: Jobs opportunities at SuperSTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both a.l.bleloch-at-liv.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: a.l.bleloch-at-liv.ac.uk
Name: Andrew Bleloch

Organization: SuperSTEM Laboratory, UK

Title-Subject: [Filtered] Jobs opportunities at SuperSTEM

Question: There are three posts at various levels available at
SuperSTEM in the UK to be found through the following link:
http://www.superstem.dl.ac.uk/

Deadline is 13 March.

Andrew Bleloch

Login Host: 148.79.162.143
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:26:17 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n251QHHp005268
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:26:17 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240803c5d4daa7afa6-at-[206.69.208.22]}
8, 11 -- Date: Wed, 4 Mar 2009 19:26:15 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: a.l.bleloch-at-liv.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Jobs opportunities at SuperSTEM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: anita.garg-at-grc.nasa.gov
Date: Wed, 4 Mar 2009 19:26:48 -0600
Subject: [Microscopy] viaWWW: TEM: Electron diffraction Simulation program for PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both anita.garg-at-grc.nasa.gov as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: anita.garg-at-grc.nasa.gov
Name: Anita Garg

Organization: NASA GRC

Title-Subject: [Filtered] TEM: Electron diffraction Simulation program for PC

Question: Dear Colleagues
What is the best electron-diffraction simulation program available
for PC these days? Earlier, the "Diffract" program used to have a Mac
version only; is there a PC version available now?
TIA,
Anita

Login Host: 128.156.10.80
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:26:48 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n251Qk9B006802
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:26:48 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c5d4dac1b5b9-at-[206.69.208.22]}
6, 11 -- Date: Wed, 4 Mar 2009 19:26:46 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: anita.garg-at-grc.nasa.gov (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM: Electron diffraction Simulation program for PC
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bfostermme-at-sbcglobal.net
Date: Wed, 4 Mar 2009 20:02:19 -0600
Subject: [Microscopy] Re: viaWWW: Objective for Dispersion Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Louie

Try McCrone Associates in Westmont, IL. They used to make a centerable center stop just for this purpose, as well as positive and negative dispersion staining. I once had the wonderful experience of acting as a teaching assistant when Dr. McCrone gave a course on this topic to the New York Microscopical Society. These centerable center stops were a bit tricky to work with, but once you get them aligned, they work beautifully.

I also recommend that you contact Dennis O'Leary (rdenol-at-aol.com). He deals in excellent second hand equipment and also has had experience with this technique.

Hope this was helpful.

Best regards,
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310 Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now running the FIRST Global study on AFM/STM use. Visit our website for details.

At 07:40 PM 3/4/2009, lbustillos-at-emsl.com wrote:




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
14, 24 -- From bfostermme-at-sbcglobal.net Wed Mar 4 20:02:18 2009
14, 24 -- Received: from smtp113.sbc.mail.mud.yahoo.com (smtp113.sbc.mail.mud.yahoo.com [68.142.198.212])
14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2522ICr013865
14, 24 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 20:02:18 -0600
14, 24 -- Message-Id: {200903050202.n2522ICr013865-at-ns.microscopy.com}
14, 24 -- Received: (qmail 99090 invoked from network); 5 Mar 2009 02:02:17 -0000
14, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 24 -- s=s1024; d=sbcglobal.net;
14, 24 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Cc:In-Reply-To:References:Mime-Version:Content-Type;
14, 24 -- b=aNV5S/8c3QODfD2jdxZTKAUO5t/0du08FvdqNqsRW4WPq5vaXHI45W/Jsi4nBzTajAk9MY7JYHbWuEjPU38q0tGDOenDxTAUNPH2ld16FUd63u03ZWgjGj92aonC8r1ALwCu/Re2A1u6jMQCxNv/xQBj4WrJNWMxx5y3dm9R+P0= ;
14, 24 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login)
14, 24 -- by smtp113.sbc.mail.mud.yahoo.com with SMTP; 5 Mar 2009 02:02:17 -0000
14, 24 -- X-YMail-OSG: LjsP83IVM1nzYytrjTVX20mMwWf9RO4XhWtLaiUdi5FIz8Hm7KWGPJtM9WTWbGvG7YjhBpEyts6f7cJ8iiGXDe9kugDV5Sl2mGf7OAWp2zF9nM7.IkMtWvAhVbL0TMkUBX35pHrD747Nh1hydIMAGH_VR8nLVGi2smQia0zz6x.okTqNlVl33UoGkVdfT5Zi8E2JcGDPqBUQLompE.djEwjLk.CnKmA-
14, 24 -- X-Yahoo-Newman-Property: ymail-3
14, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 24 -- Date: Wed, 04 Mar 2009 19:57:59 -0600
14, 24 -- To: lbustillos-at-emsl.com, microscopy-at-microscopy.com
14, 24 -- From: Barbara Foster {bfostermme-at-sbcglobal.net}
14, 24 -- Subject: Re: [Microscopy] viaWWW: Objective for Dispersion Staining
14, 24 -- Cc: "Dennis O'Leary" {rdenol-at-hotmail.com}
14, 24 -- In-Reply-To: {200903050137.n251bFTE006544-at-ns.microscopy.com}
14, 24 -- References: {200903050137.n251bFTE006544-at-ns.microscopy.com}
14, 24 -- Mime-Version: 1.0
14, 24 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Thu, 5 Mar 2009 01:33:09 -0600
Subject: [Microscopy] Operating system.. Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The microscopes I have used so far had some computer interface and all
were using some version of Microsoft Windows operating system.
Unfortunately, all the systems have crashed from time to time due to
computer problems.

In my opinion, Microsoft Windows OS needs to be rebooted periodically,
otherwise it is gonna crash in the middle of most important task. And,
you are gonna loose your best TEM images!

I have also used Linux operating system for computational work. Linux
is much more stable compared to Windows. I wonder if electron
microscope vendors ever considered using something like Linux as
operating system.

We have a new TEM from FEI and the imaging software has crashed a few
times already (this is making me nervous). The computer has only FEI
installed software; TEM server, imaging interface etc. No Internet
connection either. Hard drives are pretty much empty, as well.

I have to give a break from observation of the sample and save my
images frequently. I guess this will be a problem when someone wants
to observe and record images of kinematical process e.g. phase change,
radiation damage in TEM.

Kind Regards.
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================



On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean and!
}  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty.
}
} Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back.
}
} My 2 cents Canadian (~1.56 cents US),
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB  E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax:   506-364-2505
} email: jehrman-at-mta.ca
} www:   http://www.mta.ca/dmf
}


==============================Original Headers==============================
12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009
12, 32 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.188])
12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n257X8E3003823
12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08 -0600
12, 32 -- Received: by fk-out-0910.google.com with SMTP id z23so1377377fkz.2
12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 23:33:07 -0800 (PST)
12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
12, 32 -- :from:to:content-type:content-transfer-encoding;
12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=;
12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3
12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH+7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk
12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0=
12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
12, 32 -- :content-transfer-encoding;
12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/1MwmapyIDtWXubqnbmupaO9
12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/RzBjXBeWt2boQrxSHs66rUtU6fTzbe
12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0=
12, 32 -- MIME-Version: 1.0
12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq.36.1236238387799; Wed,
12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST)
12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300
12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com}
12, 32 -- Subject: Operating system.. Re: Esem users
12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
12, 32 -- To: microscopy {Microscopy-at-microscopy.com}
12, 32 -- Content-Type: text/plain; charset=UTF-8
12, 32 -- Content-Transfer-Encoding: 8bit
12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n257X8E3003823
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Thu, 5 Mar 2009 02:29:53 -0600
Subject: [Microscopy] Operating system.. Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ayten;
My opinion is that FEI tries to do too much with one computer. Our 11 year old JEOL 2010F runs one computer (XP) dedicated to Gatan Digital Micrograph (imaging and EELS), one computer (Win 2000) dedicated to FASTEM, which runs the microscope, and one computer (XP) to run the EDX system (Noran). Ethernet communicates between the three to carry system information like magnification and imaging mode. We NEVER have to reboot these computers and we NEVER have system freezes while running the microscope. Plus, the microscope is operated with knobs after just a few clicks to set it up. Am I just lucky or what?

John Mardinly,
Numonyx

-----Original Message-----
X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
Sent: Wednesday, March 04, 2009 11:40 PM
To: MARDINLY, A

The microscopes I have used so far had some computer interface and all
were using some version of Microsoft Windows operating system.
Unfortunately, all the systems have crashed from time to time due to
computer problems.

In my opinion, Microsoft Windows OS needs to be rebooted periodically,
otherwise it is gonna crash in the middle of most important task. And,
you are gonna loose your best TEM images!

I have also used Linux operating system for computational work. Linux
is much more stable compared to Windows. I wonder if electron
microscope vendors ever considered using something like Linux as
operating system.

We have a new TEM from FEI and the imaging software has crashed a few
times already (this is making me nervous). The computer has only FEI
installed software; TEM server, imaging interface etc. No Internet
connection either. Hard drives are pretty much empty, as well.

I have to give a break from observation of the sample and save my
images frequently. I guess this will be a problem when someone wants
to observe and record images of kinematical process e.g. phase change,
radiation damage in TEM.

Kind Regards.
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================



On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean a!
nd!
}  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty.
}
} Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back.
}
} My 2 cents Canadian (~1.56 cents US),
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB  E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax:   506-364-2505
} email: jehrman-at-mta.ca
} www:   http://www.mta.ca/dmf
}


==============================Original Headers==============================
12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009
12, 32 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.188])
12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n257X8E3003823
12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08 -0600
12, 32 -- Received: by fk-out-0910.google.com with SMTP id z23so1377377fkz.2
12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 23:33:07 -0800 (PST)
12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
12, 32 -- :from:to:content-type:content-transfer-encoding;
12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=;
12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3
12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH+7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk
12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0=
12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
12, 32 -- :content-transfer-encoding;
12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/1MwmapyIDtWXubqnbmupaO9
12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/RzBjXBeWt2boQrxSHs66rUtU6fTzbe
12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0=
12, 32 -- MIME-Version: 1.0
12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq.36.1236238387799; Wed,
12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST)
12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300
12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com}
12, 32 -- Subject: Operating system.. Re: Esem users
12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
12, 32 -- To: microscopy {Microscopy-at-microscopy.com}
12, 32 -- Content-Type: text/plain; charset=UTF-8
12, 32 -- Content-Transfer-Encoding: 8bit
12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n257X8E3003823
==============================End of - Headers==============================



==============================Original Headers==============================
21, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 5 02:29:53 2009
21, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n258TrW8018802
21, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 5 Mar 2009 02:29:53 -0600
21, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
21, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 8D05D1440A2;
21, 29 -- Thu, 5 Mar 2009 01:34:05 -0500 (EST)
21, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
21, 29 -- Thu, 5 Mar 2009 03:29:52 -0500
21, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 29 -- Content-class: urn:content-classes:message
21, 29 -- MIME-Version: 1.0
21, 29 -- Content-Type: text/plain;
21, 29 -- charset="iso-8859-1"
21, 29 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users
21, 29 -- Date: Thu, 5 Mar 2009 03:29:27 -0500
21, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE95D6-at-EXDRESBENMX012.numonyx.local}
21, 29 -- In-Reply-To: {200903050740.n257eMap015286-at-ns.microscopy.com}
21, 29 -- X-MS-Has-Attach:
21, 29 -- X-MS-TNEF-Correlator:
21, 29 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users
21, 29 -- Thread-Index: AcmdZauU4H31zME3SlyzDz5WmfaahgABTAcg
21, 29 -- References: {200903050740.n257eMap015286-at-ns.microscopy.com}
21, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
21, 29 -- To: {celikaktas-at-gmail.com}
21, 29 -- Cc: {Microscopy-at-Microscopy.com}
21, 29 -- X-OriginalArrivalTime: 05 Mar 2009 08:29:52.0130 (UTC) FILETIME=[8E021620:01C99D6C]
21, 29 -- Content-Transfer-Encoding: 8bit
21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n258TrW8018802
==============================End of - Headers==============================




From: eikonika-at-otenet.gr
Date: Thu, 5 Mar 2009 04:49:07 -0600
Subject: [Microscopy] ESEM users, another sad story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers

Here is another sad story on a PC controlled microscope. Recently I bought
a second hand SEM. At that time I was not a member of the listserver and
had no idea about the PC-EM problems. When the graphic card was broken, the
microscope¹s company was unable to find a replace one for me, although the
scope is no more than 10 years old. Fortunately I found one (actually two)
through the web and the scope works again.

But PC problems are continuing. The microscope operating system is one out
of tens or maybe hundreds of different versions being around, for the same
model! My scope came from the USA with a starting CD of different version
than the one already installed in the scope. Although the scope was
decommissioned by the company, the company was unable to find for me the
correct version. People from the company in Europe don¹t even believe that
the scope works on windows 98 and not on NT. A real nightmareŠ

I would like to ask the list if anybody knows somebody who can install a
new operating system. A friend in this list told me about a German company
that computerizes old microscopes by installing new scan coils. However, if
the scope is already computerized like mine, is maybe more difficult.

Also, I would like to propose if there is room for some sort of world-wide
union between EM owners who have PC controlled scopes of several yeas old.
Such a union could help giving solutions outside the microscope companies
or even demand from the companies to keep PC spare parts for longer time
etc.

Regards

yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
Mobile +30 6945 107477
eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com






==============================Original Headers==============================
13, 21 -- From eikonika-at-otenet.gr Thu Mar 5 04:49:07 2009
13, 21 -- Received: from rosebud.otenet.gr (rosebud.otenet.gr [83.235.67.32])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25An6PE003561
13, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 04:49:06 -0600
13, 21 -- Received: from [192.168.1.34] (athedsl-259835.home.otenet.gr [85.73.62.153])
13, 21 -- (authenticated bits=0)
13, 21 -- by rosebud.otenet.gr (8.13.8/8.13.8/Debian-3) with ESMTP id n25An5pf020960
13, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 12:49:05 +0200
13, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122
13, 21 -- Date: Thu, 05 Mar 2009 12:49:04 +0200
13, 21 -- Subject: ESEM users, another sad story
13, 21 -- From: yorgos nikas {eikonika-at-otenet.gr}
13, 21 -- To: {Microscopy-at-microscopy.com}
13, 21 -- Message-ID: {C5D57AC0.2EF4%eikonika-at-otenet.gr}
13, 21 -- Thread-Topic: ESEM users, another sad story
13, 21 -- Thread-Index: AcmdgAAcPuByEglzEd6GAgAdT0jQTA==
13, 21 -- Mime-version: 1.0
13, 21 -- Content-type: text/plain;
13, 21 -- charset="ISO-8859-1"
13, 21 -- Content-Transfer-Encoding: 8bit
13, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25An6PE003561
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 5 Mar 2009 06:05:02 -0600
Subject: [Microscopy] Re: other not so sad story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Let me kick another thought into this discussion: most companies management
wants computerized equipment. Why? They perceive then as less expensive
to operate. No need for several experts at high salary and benefits,
perhaps not even one. The tech rep sets it up and each user just walks up
and uses it. Can't screw up the settings, cause the computer "remembers"
the original settings.

I've see this with GC/MS, with IR, with image analysis equipment with TEMs
and SEMs. Even the adds for this equipment suggest at "expert in a box"
with their equipment.

now I'm not saying they are total to blame... I've heard sales pitches to
management about justification for a piece of equipment by claiming the
ease of operation in a multi-user environment: "blab blab..free standing,
user friendly and we don't need dedicated operators....blab blab." I
suspect we've even used this argument to get equipment we needed.

I needed a new EDS system at Degussa, I didn't select the best, I selected
one that was the most user friendly 'cause I could justify it.

So sign me up for the Luddite Microscopy Group (humm..maybe a new Linked-In
group), but I know to some small degree I'm responsible.

stay safe.......
Frank
Reformed Luddite Microscopist

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
8, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 5 06:05:01 2009
8, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25C515R019105
8, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 06:05:01 -0600
8, 22 -- In-Reply-To: {200903051057.n25AvaOA016153-at-ns.microscopy.com}
8, 22 -- Subject: Re: other not so sad story
8, 22 -- To: Microscopy-at-microscopy.com
8, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
8, 22 -- Message-ID: {OFE15843D7.2A79266A-ON85257570.0040DCFA-85257570.00425949-at-lincolnelectric.com}
8, 22 -- Date: Thu, 5 Mar 2009 07:04:42 -0500
8, 22 -- From: Frank_Karl-at-lincolnelectric.com
8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- CD-MIME complete at 03/05/2009 07:04:43 AM,
8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- Serialize complete at 03/05/2009 07:04:43 AM
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain;
8, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: bfostermme-at-sbcglobal.net
Date: Thu, 5 Mar 2009 07:19:20 -0600
Subject: [Microscopy] Re: ESEM users, another sad story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mary

if you're talking about the upgrade from Windows NT 4 to XP then I am
aware of it and if all goes well we hope to arrange it in the future.
In fact, despite the cost (probably about 1/10 of original
microscope), I get the impression that not all manufacturers offer
similar upgrades so full marks to Hitachi.

I hasten to add that I have no association with Hitachi other than as
a satisfied customer.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: Mary Fletcher {maryflet-at-interchange.ubc.ca}

Hi, Yorgos,

SemTech Solutions, near Boston, "rehabilitates" old EMs but gutting the computer system and updating them. I don't know if they do any work outside the US but they have an excellent reputation. Contact Gerry O'Loughlin (gerry-at-semtechsolutions.com).

Hope this was helpful.
Barbara Foster

Use AFM/ STM/ or NSOM? Take part in the first GLOBAL AFM/STM study. Deatails at www.MicroscopyEducation.com

Barbara Foster, President and Sr. Consultant
Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310 Skype: fostermme
W: www.MicroscopyEducation.com


/At 05:00 AM 3/5/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
12, 25 -- From bfostermme-at-sbcglobal.net Thu Mar 5 07:19:20 2009
12, 25 -- Received: from smtp103.sbc.mail.mud.yahoo.com (smtp103.sbc.mail.mud.yahoo.com [68.142.198.202])
12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n25DJHBl015568
12, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 07:19:19 -0600
12, 25 -- Message-Id: {200903051319.n25DJHBl015568-at-ns.microscopy.com}
12, 25 -- Received: (qmail 41944 invoked from network); 5 Mar 2009 13:19:16 -0000
12, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 25 -- s=s1024; d=sbcglobal.net;
12, 25 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Cc:In-Reply-To:References:Mime-Version:Content-Type:Content-Transfer-Encoding;
12, 25 -- b=Il1htlMG/N2goJu87W7deXzWKfKgL6L0vwHO3MblP69vRZZCxBCVizeuX7wKNtMRtg4sz0KBxXelw9v0t3M4mQa8S2rFDGAj4FIBtZhiW16HOwcMzCCbLzGrwnYuvOI7e0AfTEYfqBpqBvmTbC4k4KJNw1bCINgqcRhs/P8HlsM= ;
12, 25 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login)
12, 25 -- by smtp103.sbc.mail.mud.yahoo.com with SMTP; 5 Mar 2009 13:19:15 -0000
12, 25 -- X-YMail-OSG: mrmqHZAVM1me.BfX86abUjDAiDz8obaF6q.Tgui28IJ4tys0oCpo.HCBgo7vNHVr2WwvokaUc.lkamuv8cfRlqFVdtuxPIFuL2Z5lqLinA1ibtcMKwoONhtWtMxkjzNusnohA2APdPI9ZIsT.A_lL2e7bmTcOFQQQR6G0UJiIShmj6KN8a5obd5P68omwBWARZ7k1w3gNlX5SIJ23NSs_etiapLsM7E-
12, 25 -- X-Yahoo-Newman-Property: ymail-3
12, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 25 -- Date: Thu, 05 Mar 2009 07:14:54 -0600
12, 25 -- To: eikonika-at-otenet.gr, microscopy-at-microscopy.com
12, 25 -- From: Barbara Foster {bfostermme-at-sbcglobal.net}
12, 25 -- Subject: Re: [Microscopy] ESEM users, another sad story
12, 25 -- Cc: gerry-at-semtechsolutions.com
12, 25 -- In-Reply-To: {200903051056.n25AuvwO015036-at-ns.microscopy.com}
12, 25 -- References: {200903051056.n25AuvwO015036-at-ns.microscopy.com}
12, 25 -- Mime-Version: 1.0
12, 25 -- Content-Type: text/plain; charset="iso-8859-1"
12, 25 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Thu, 5 Mar 2009 07:59:04 -0600
Subject: [Microscopy] Operating system.. Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

You should be playing in Vegas instead of doing microscopy.

You are the only person I have heard of who uses Microsoft operating
system and had never have to reboot a computer.

I must admit that we have all FEI microscopes and we are not free of
computer problems.

We have an XL30 system that was upgraded last year from a DX4
configuration (originally Win3.1) to a system with two computers, one
dedicated exclusively to control the SEM running Win2000, another one
running our EDS system. The SEM computer is not connected to the
internet, it communicates with the EDS PC via network. No user files
or data are stored there nor any devices are allowed to be connected
to it. Still we have to reboot the computer about once every two weeks
or so. The second computer runs WinXP and is connected to the internet
and users can store and transfer data to and from it. It also freezes
couple of times each month.

Then we have a Tecnai12 TEM. It uses one computer (Win2000) running
the TEM and two Gatan cameras, it too crashes from time to time. The
worst part about this system is that the TEM vacuum system has to be
restarted completely every time the computer reboots. Really bad
design decision from FEI.

Also we have a CM300 TEM connected to another Win2000 computer. It
does not run any TEM control software from FEI it only runs EDS and
Gatan software. It also requires rebooting and I must say not
significantly more frequently that the computers running FEI software.
So my experience suggests it is Microsoft than anything else.
We had the CM300 originally connected to a Macintosh which was running
our EDS and Gatan software. We had to abandon it because EDAX and
Gatan stopped support for Macintosh.
During the two years that the system was running under the old Mac
OS8 I did not have to reboot the system except when I did operating
system upgrade or a new software installation on the Mac.

I wish I could have all our systems running and supported by MacOS,
although It appears that since the adoption of Intel processors in
the Macs or/and maybe the complexity of the new operating systems even
Macs are not that reliable anymore.

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu



On Mar 5, 2009, at 12:34 AM, A.MARDINLY-at-numonyx.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Ayten;
} My opinion is that FEI tries to do too much with one computer. Our
} 11 year old JEOL 2010F runs one computer (XP) dedicated to Gatan
} Digital Micrograph (imaging and EELS), one computer (Win 2000)
} dedicated to FASTEM, which runs the microscope, and one computer
} (XP) to run the EDX system (Noran). Ethernet communicates between
} the three to carry system information like magnification and imaging
} mode. We NEVER have to reboot these computers and we NEVER have
} system freezes while running the microscope. Plus, the microscope is
} operated with knobs after just a few clicks to set it up. Am I just
} lucky or what?
}
} John Mardinly,
} Numonyx
}
} -----Original Message-----
} X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
} Sent: Wednesday, March 04, 2009 11:40 PM
} To: MARDINLY, A
} Subject: [Microscopy] Operating system.. Re: Esem users
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} The microscopes I have used so far had some computer interface and all
} were using some version of Microsoft Windows operating system.
} Unfortunately, all the systems have crashed from time to time due to
} computer problems.
}
} In my opinion, Microsoft Windows OS needs to be rebooted periodically,
} otherwise it is gonna crash in the middle of most important task. And,
} you are gonna loose your best TEM images!
}
} I have also used Linux operating system for computational work. Linux
} is much more stable compared to Windows. I wonder if electron
} microscope vendors ever considered using something like Linux as
} operating system.
}
} We have a new TEM from FEI and the imaging software has crashed a few
} times already (this is making me nervous). The computer has only FEI
} installed software; TEM server, imaging interface etc. No Internet
} connection either. Hard drives are pretty much empty, as well.
}
} I have to give a break from observation of the sample and save my
} images frequently. I guess this will be a problem when someone wants
} to observe and record images of kinematical process e.g. phase change,
} radiation damage in TEM.
}
} Kind Regards.
} Ayten.
}
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
}
}
} On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Well, put me in the non-Luddite camp. Sure, I think you need knobs
} } for "touchy-feely" operations like focus and stigmation, but most
} } of the other things I'm happy to have under the control of the
} } computer because then I can automate them if I have to. Macro-
} } Express runs my SEM. Now it does take a little troubleshooting and
} } testing to get everything to work right, but it's very liberating
} } to hit one virtual button and have a whole slew of routine things
} } done for you while doing something else. I easily outstrip the
} } productivity I used to consider quite respectable back in the
} } analog days. In my limited experience in other labs with computer
} } controlled equipment, I've found a lot of their troubles stem from
} } treating the dedicated computer on the equipment just like any
} } other computer. One I saw was so crammed with Internet chat, games
} } and screensaver programs the scope software barely had room to turn
} } around, let alone run the scope. My first rule - keep the computer
} } clean a!
} nd!
} } only install what you absolutely need - farm everything else out
} } to normal desktops. Only install one new thing at a time, and run
} } the scope software for quite awhile (a week or more) until you're
} } absolutely sure it's not gumming up the works. With this strategy I
} } have a crash or lockup of the system on average about once a year,
} } usually because I'm doing something stupid that I should know
} } better not to do at that particular time. My SEM just turned 10
} } years old last year, and I fully expect it to keep going for at
} } least another 10 without any tremendous difficulty.
} }
} } Oh, and of course it does help to have a person around with some
} } experience and interest in computers. If you hate the things to
} } begin with, they're probably going to respond by hating you back.
} }
} } My 2 cents Canadian (~1.56 cents US),
} }
} } Jim
} }
} } --
} }
} } James M. Ehrman
} } Digital Microscopy Facility
} } Mount Allison University
} } 63B York St.
} } Sackville, NB E4L 1G7
} } CANADA
} }
} } phone: 506-364-2519
} } fax: 506-364-2505
} } email: jehrman-at-mta.ca
} } www: http://www.mta.ca/dmf
} }
}
}
} ==============================Original
} Headers==============================
} 12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009
} 12, 32 -- Received: from fk-out-0910.google.com (fk-
} out-0910.google.com [209.85.128.188])
} 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n257X8E3003823
} 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08
} -0600
} 12, 32 -- Received: by fk-out-0910.google.com with SMTP id
} z23so1377377fkz.2
} 12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009
} 23:33:07 -0800 (PST)
} 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 12, 32 -- d=gmail.com; s=gamma;
} 12, 32 -- h=domainkey-signature:mime-
} version:received:date:message-id:subject
} 12, 32 -- :from:to:content-type:content-transfer-encoding;
} 12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=;
} 12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/
} tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3
} 12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH
} +7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk
} 12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0=
} 12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 12, 32 -- d=gmail.com; s=gamma;
} 12, 32 -- h=mime-version:date:message-
} id:subject:from:to:content-type
} 12, 32 -- :content-transfer-encoding;
} 12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/
} 1MwmapyIDtWXubqnbmupaO9
} 12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/
} RzBjXBeWt2boQrxSHs66rUtU6fTzbe
} 12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0=
} 12, 32 -- MIME-Version: 1.0
} 12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq.
} 36.1236238387799; Wed,
} 12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST)
} 12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300
} 12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com
} }
} 12, 32 -- Subject: Operating system.. Re: Esem users
} 12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
} 12, 32 -- To: microscopy {Microscopy-at-microscopy.com}
} 12, 32 -- Content-Type: text/plain; charset=UTF-8
} 12, 32 -- Content-Transfer-Encoding: 8bit
} 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n257X8E3003823
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 21, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 5 02:29:53 2009
} 21, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net
} (mail1.numonyx.com [57.77.12.37])
} 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n258TrW8018802
} 21, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 5 Mar 2009 02:29:53
} -0600
} 21, 29 -- Received: from exdresfenmx01.numonyx.local (unknown
} [10.96.252.22])
} 21, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with
} ESMTP id 8D05D1440A2;
} 21, 29 -- Thu, 5 Mar 2009 01:34:05 -0500 (EST)
} 21, 29 -- Received: from EXDRESBENMX012.numonyx.local
} ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft
} SMTPSVC(6.0.3790.3959);
} 21, 29 -- Thu, 5 Mar 2009 03:29:52 -0500
} 21, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 21, 29 -- Content-class: urn:content-classes:message
} 21, 29 -- MIME-Version: 1.0
} 21, 29 -- Content-Type: text/plain;
} 21, 29 -- charset="iso-8859-1"
} 21, 29 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users
} 21, 29 -- Date: Thu, 5 Mar 2009 03:29:27 -0500
} 21, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE95D6-at-EXDRESBENMX012.numonyx.local
} }
} 21, 29 -- In-Reply-To: {200903050740.n257eMap015286-at-ns.microscopy.com}
} 21, 29 -- X-MS-Has-Attach:
} 21, 29 -- X-MS-TNEF-Correlator:
} 21, 29 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users
} 21, 29 -- Thread-Index: AcmdZauU4H31zME3SlyzDz5WmfaahgABTAcg
} 21, 29 -- References: {200903050740.n257eMap015286-at-ns.microscopy.com}
} 21, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
} 21, 29 -- To: {celikaktas-at-gmail.com}
} 21, 29 -- Cc: {Microscopy-at-Microscopy.com}
} 21, 29 -- X-OriginalArrivalTime: 05 Mar 2009 08:29:52.0130 (UTC)
} FILETIME=[8E021620:01C99D6C]
} 21, 29 -- Content-Transfer-Encoding: 8bit
} 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n258TrW8018802
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
15, 28 -- From bozhilov-at-ucr.edu Thu Mar 5 07:59:04 2009
15, 28 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25Dx3TR030282
15, 28 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 07:59:04 -0600
15, 28 -- Received: from [192.168.100.104] (71-80-136-179.static.hspr.ca.charter.com [71.80.136.179] (may be forged))
15, 28 -- by sentrell.ucr.edu (MOS 3.10.3-GA)
15, 28 -- with ESMTP id DKN20649 (AUTH bozhilov-at-ucr.edu);
15, 28 -- Thu, 5 Mar 2009 05:58:59 -0800 (PST)
15, 28 -- Cc: KN Bozhilov {bozhilov-at-ucr.edu}
15, 28 -- Message-Id: {FA15111A-00BA-4549-B91F-F0AED37BB43B-at-ucr.edu}
15, 28 -- From: KN Bozhilov {bozhilov-at-ucr.edu}
15, 28 -- To: A.MARDINLY-at-numonyx.com, microscopy-at-microscopy.com
15, 28 -- In-Reply-To: {200903050834.n258Yncg029004-at-ns.microscopy.com}
15, 28 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
15, 28 -- Content-Transfer-Encoding: 7bit
15, 28 -- Mime-Version: 1.0 (Apple Message framework v930.3)
15, 28 -- Subject: Re: [Microscopy] RE: Operating system.. Re: Esem users
15, 28 -- Date: Thu, 5 Mar 2009 05:58:59 -0800
15, 28 -- References: {200903050834.n258Yncg029004-at-ns.microscopy.com}
15, 28 -- X-Mailer: Apple Mail (2.930.3)
15, 28 -- X-Junkmail-SD-Raw: score=unknown,
15, 28 -- refid=str=0001.0A09020A.49AFDAA5.0181,ss=1,fgs=0,
15, 28 -- ip=192.168.100.104,
15, 28 -- so=2008-09-22 23:22:13,
15, 28 -- dmn=5.7.1/2008-09-02,
15, 28 -- mode=single engine
15, 28 -- X-Junkmail-IWF: false
15, 28 -- X-Junkmail-Status: score=0/50, host=sentrell.ucr.edu
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 5 Mar 2009 08:36:48 -0600
Subject: [Microscopy] Re: other not so sad story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,
You're right. The management types who have never actually done the work in
their respective industries don't understand or appreciate the value of
"professionals".

For many years I've told people that the SEM is NOT a microscope, despite
the name. It is a signal generator and one or more signal processors. You
tell me what you want to see and I can probably show it to you. Then we can
have a long talk about what is real.

High levels of automation first started showing up in EDS systems. As most
everyone on this Listserver is aware, there are so many variables involved
in x-ray analysis that one MUST understand what is going on with the
equipment and the sample. Using an auto-ident function almost always brings
up a number of obviously incorrect element identifications. Most of us have
heard at least one person say, "But the computer says..." My response is
always, "I don't give a damn what the computer says."

This is now aggravated by the high level of automation in the SEMs and the
"anyone can run this" attitude that not only disrespects the hard-earned
expertise of professional microscopists, but has the potential to introduce
huge amounts of essentially bogus data to both scientific and industrial
data bases and even "expert systems".

Automation is a wonderful thing, provided you understand the underlying
equipment, processes and theories. Yes, it is theoretically possible to
create the Encyclopedia Britannica with a huge number of primates banging on
keyboards, but it is much more efficient, and has a far higher likelihood of
succeeding, if it is created by a small group of humans, expert in their
respective fields. The "trouble" is that you have to pay them more than
other primates.

We've seen what "financial experts" have done to the world's financial
systems. How do we keep the "bean counters" from doing to same thing to all
the other industries? How do we make them realize that their most expensive
resource (labor) is the most expensive because it is the most valuable
resource they have at their disposal?

Sorry about the rant. I feel better, though.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com]

Sent: Thursday, March 05, 2009 7:07 AM
To: kenconverse-at-qualityimages.biz

Let me kick another thought into this discussion: most companies management
wants computerized equipment. Why? They perceive then as less expensive
to operate. No need for several experts at high salary and benefits,
perhaps not even one. The tech rep sets it up and each user just walks up
and uses it. Can't screw up the settings, cause the computer "remembers"
the original settings.

I've see this with GC/MS, with IR, with image analysis equipment with TEMs
and SEMs. Even the adds for this equipment suggest at "expert in a box"
with their equipment.

now I'm not saying they are total to blame... I've heard sales pitches to
management about justification for a piece of equipment by claiming the
ease of operation in a multi-user environment: "blab blab..free standing,
user friendly and we don't need dedicated operators....blab blab." I
suspect we've even used this argument to get equipment we needed.

I needed a new EDS system at Degussa, I didn't select the best, I selected
one that was the most user friendly 'cause I could justify it.

So sign me up for the Luddite Microscopy Group (humm..maybe a new Linked-In
group), but I know to some small degree I'm responsible.

stay safe.......
Frank
Reformed Luddite Microscopist

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
8, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 5 06:05:01 2009
8, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com
[64.109.211.115])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n25C515R019105
8, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 06:05:01
-0600
8, 22 -- In-Reply-To: {200903051057.n25AvaOA016153-at-ns.microscopy.com}
8, 22 -- Subject: Re: other not so sad story
8, 22 -- To: Microscopy-at-microscopy.com
8, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
8, 22 -- Message-ID:
{OFE15843D7.2A79266A-ON85257570.0040DCFA-85257570.00425949-at-lincolnelectric.c
om}
8, 22 -- Date: Thu, 5 Mar 2009 07:04:42 -0500
8, 22 -- From: Frank_Karl-at-lincolnelectric.com
8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- CD-MIME complete at 03/05/2009 07:04:43 AM,
8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release
8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release
8.0.1|February
8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM,
8, 22 -- Serialize complete at 03/05/2009 07:04:43 AM
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain;
8, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




==============================Original Headers==============================
27, 26 -- From kenconverse-at-qualityimages.biz Thu Mar 5 08:36:47 2009
27, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25EaleX012662
27, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 08:36:47 -0600
27, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta06.mail.rr.com
27, 26 -- with ESMTP
27, 26 -- id {20090305143647.DRGZ3201.cdptpa-omta06.mail.rr.com-at-Ken} ;
27, 26 -- Thu, 5 Mar 2009 14:36:47 +0000
27, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
27, 26 -- To: {Frank_Karl-at-lincolnelectric.com} ,
27, 26 -- "MSA Listserver" {microscopy-at-microscopy.com}
27, 26 -- Subject: RE: [Microscopy] Re: other not so sad story
27, 26 -- Date: Thu, 5 Mar 2009 09:36:40 -0500
27, 26 -- Message-ID: {D58AF4A5C85046AC8DF69AED190BCAB7-at-Ken}
27, 26 -- MIME-Version: 1.0
27, 26 -- Content-Type: text/plain;
27, 26 -- charset="us-ascii"
27, 26 -- X-Priority: 3 (Normal)
27, 26 -- X-MSMail-Priority: Normal
27, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
27, 26 -- Importance: Normal
27, 26 -- Thread-Index: Acmdiuwq5OJiRTcSRuufg7Yilj7KjgAEKRcw
27, 26 -- In-Reply-To: {200903051207.n25C7CKO023060-at-ns.microscopy.com}
27, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
27, 26 -- Content-Transfer-Encoding: 8bit
27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25EaleX012662
==============================End of - Headers==============================




From: wadowska-at-upei.ca
Date: Thu, 5 Mar 2009 09:05:37 -0600
Subject: [Microscopy] viaWWW: TEM digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: UPEI/AVC

Title-Subject: [Filtered] TEM digital camera

Question: Hello,
We have H7500 with AMT XR 40 digital camera. Just recently we started
to have problems viewing images captured by the camera. Half of the
computer screen was black. It turned out that aluminum foil that
covers the screen broke off in half. We have to buy a new screen
(ouch, expensive). We used this system only for a bit more then two
years and I was wondering if anybody had similar problem and what
might be the cause of it.
TIA
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Mar 5 09:05:37 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25F5Y9q027100
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 09:05:36 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c5d59aadb1f7-at-[206.69.208.22]}
6, 11 -- Date: Thu, 5 Mar 2009 09:05:33 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM digital camera
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Thu, 5 Mar 2009 11:09:23 -0600
Subject: [Microscopy] Operating system.. Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I wonder if the problem traces back to some of the earlier days of instrument control. I recall that programs were written to directly access memory location to transfer data and control the instrument. Those interfaces were not necessarily in the form of a well-written device driver. Programs sometimes got out of control and changed a byte or two where they shouldn't have and the system came crashing down.

I would like to think that things have gotten better and that the software is written more carefully or that maybe there are some safeguards in place. The number of crashes I experience has dropped a lot since the early 1980s. However, I still have some software with various issues. It is just not quite so dramatic.

I suppose this is the reason that computers are dedicated to each function. Let one computer control the microscope, another the EDS, and another the web surfing. If the EM control program goes awry, it won't take out my browser session - or vice versa.

Warren

-----Original Message-----
X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
Sent: Thursday, March 05, 2009 1:34 AM
To: wesaia-at-iastate.edu

The microscopes I have used so far had some computer interface and all
were using some version of Microsoft Windows operating system.
Unfortunately, all the systems have crashed from time to time due to
computer problems.

In my opinion, Microsoft Windows OS needs to be rebooted periodically,
otherwise it is gonna crash in the middle of most important task. And,
you are gonna loose your best TEM images!

I have also used Linux operating system for computational work. Linux
is much more stable compared to Windows. I wonder if electron
microscope vendors ever considered using something like Linux as
operating system.

We have a new TEM from FEI and the imaging software has crashed a few
times already (this is making me nervous). The computer has only FEI
installed software; TEM server, imaging interface etc. No Internet
connection either. Hard drives are pretty much empty, as well.

I have to give a break from observation of the sample and save my
images frequently. I guess this will be a problem when someone wants
to observe and record images of kinematical process e.g. phase change,
radiation damage in TEM.

Kind Regards.
Ayten.


--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================



On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean a!
nd!
}  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty.
}
} Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back.
}
} My 2 cents Canadian (~1.56 cents US),
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB  E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax:   506-364-2505
} email: jehrman-at-mta.ca
} www:   http://www.mta.ca/dmf


==============================Original Headers==============================
21, 36 -- From wesaia-at-iastate.edu Thu Mar 5 11:09:23 2009
21, 36 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14])
21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25H9LGd012726
21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:22 -0600
21, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
21, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n25H9KaB013662
21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:20 -0600
21, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
21, 36 -- id 1172_a03d9d7c_09a6_11de_b857_001372578af6;
21, 36 -- Thu, 05 Mar 2009 10:56:51 -0600
21, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
21, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n25H9H7h011739
21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:18 -0600
21, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
21, 36 -- Thu, 5 Mar 2009 11:09:19 -0600
21, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 36 -- Content-class: urn:content-classes:message
21, 36 -- MIME-Version: 1.0
21, 36 -- Content-Type: text/plain;
21, 36 -- charset="iso-8859-1"
21, 36 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users
21, 36 -- Date: Thu, 5 Mar 2009 11:09:41 -0600
21, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703B2A3C1-at-maire.eng.iastate.edu}
21, 36 -- In-Reply-To: {200903050733.n257Xupp004878-at-ns.microscopy.com}
21, 36 -- X-MS-Has-Attach:
21, 36 -- X-MS-TNEF-Correlator:
21, 36 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users
21, 36 -- Thread-Index: AcmdZL9bEe/2u+hdS2ardWh/V4LUSAAT2u7A
21, 36 -- References: {200903050733.n257Xupp004878-at-ns.microscopy.com}
21, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
21, 36 -- To: {Microscopy-at-microscopy.com}
21, 36 -- X-OriginalArrivalTime: 05 Mar 2009 17:09:19.0886 (UTC) FILETIME=[1F703AE0:01C99DB5]
21, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.5.165527
21, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_AGE_BODY 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NUM 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0'
21, 36 -- Content-Transfer-Encoding: 8bit
21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25H9LGd012726
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Thu, 5 Mar 2009 11:43:40 -0600
Subject: [Microscopy] Regarding FEI Computers and Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I thought I would throw my thoughts into the ring here since this
seems to be an issue with everyone.  I have a unique perspective on
the FEI systems as I was working as a Field Service Engineer up until
last summer.  I'm now sitting in your seats operating an FEI system.
I have a FIB820 which runs on Windows 3.11 for WorkGroups.  In the
past few months using this system I've found this computer and Windows
3.11 need rebooting every week.  Without the reboot the system tends
to have UI system errors and slows down considerably.  This system
though is not upgradable and there are no PC spares. So once the PC
dies I'll be searching far and wide for something that runs Win 3.11,
or some upgrade from another company so I can be back up and running.

With regards to the new systems though. In the field I found that the
problem wasn't generally with FEI's software. The problem usually was
with the PC's that were used. Most of the newer systems we're using
HP PC's. These seemed to have motherboard problems which would cause
re-booting, software errors, slowdowns, and lockups. Once the PC was
replaced everything was fine.  Most newer systems were using XP, and
some on 2000. These were mainly on the Quanta and Nova systems. Also
the operators inadvertently tended to cause some of their own problems
by changing settings and loading other software.

The older systems such as the 235's, 800's, and 1200's all used
Industrial PC's mainly now the Advantec (Black Box). These ran
everything from Win 3.1 to XP and made systems upgradable as long as
the software was made to work with the system and in some cases EPROMS
and communications boards upgraded. Again the problems encountered
were generally related to Windows and misc. PC problems and not the
FEI software. Sometimes on these the SCSI system can cause reboots
and lockups.

XL series and systems like the 820 all use "different" PC's. The
components and the Windows software seem to vary greatly. Some can be
upgraded to Win2000 and others like mine seem to be stuck at whatever
they're running.  The good news is that these PC's seem to be better
built then the newer ones as this one on my 820 has been running for
10+ years.

As for systems running Linux/Unix. Applied materials SemVision
systems did use a Unix based system and would have been very stable if
the AMAT user software had been better. The world would be a better
place if run on Linux or Mac's before the Intel days.

We also have an Hitachi SEM here which has excellent resolution but
the UI and the Hardware are not user friendly or very well designed
when compared to FEI's systems. At least in my opinion, which
obviously is tilted toward FEI. It does use Windows 2000/XP shell at
least and seems to be stable enough that reboots are very few.

FEI systems and software to me are still the best out there though.
The biggest problem I see is Microsoft and PC's, along with each
manufacturers method of communicating with Hardware. If quality PC's
were used along with Windows XP and drivers/software that was well
written and debugged I don't think any of these systems would have
need of any more rebooting then each of our Desktop PC's running XP.
The WinXP PC I'm typing this is on now runs worse then the FIB820's
Win3.11 PC and requires rebooting regularly. So consider how much you
reboot your Home or Office PC and compare that to your microscope.


==============================Original Headers==============================
8, 32 -- From ian.drucker-at-gmail.com Thu Mar 5 11:43:40 2009
8, 32 -- Received: from mail-fx0-f174.google.com (mail-fx0-f174.google.com [209.85.220.174])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25HhcE1027186
8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:43:39 -0600
8, 32 -- Received: by fxm22 with SMTP id 22so28706fxm.18
8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 05 Mar 2009 09:43:36 -0800 (PST)
8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 32 -- d=gmail.com; s=gamma;
8, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
8, 32 -- :from:to:content-type:content-transfer-encoding;
8, 32 -- bh=p7cRkFdLmSxFt8oaJCs03AVMcFal7dewkTwJgsVzXLo=;
8, 32 -- b=lF4I30zETzMnu1bo6A1gAo7jhZR3N6ZKntx+IdgRONoopEosImQf9tTTP+e4Urmh80
8, 32 -- bUQ7KmqNNUag+KFVrRb+h2av7zHq8VWcIF9vYba0+N7FoI5rlgqfSa/2yKZ0ZOzqMIgR
8, 32 -- Q+yMxJ8hmZx6ZPS+ISObFLNj5ylFom5CbH0Qw=
8, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 32 -- d=gmail.com; s=gamma;
8, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
8, 32 -- :content-transfer-encoding;
8, 32 -- b=vXD6lcoG/LeA0HCp9oV+Ho9OZV/T8oqFSHalI3HJ46fDETVPYR7BL6BzrKzxT1QjbT
8, 32 -- cfBHQOYCRcvLecHQz/DfMiQNm4YCwJaUPITiFMDIsODhZopQHGK4bjW/Fyt6Rq7jjBvW
8, 32 -- 2Ei7EFqtILeEzIinTRJlNpwFBaNDK4XTvbn90=
8, 32 -- MIME-Version: 1.0
8, 32 -- Received: by 10.103.246.1 with SMTP id y1mr648585mur.116.1236275016435; Thu,
8, 32 -- 05 Mar 2009 09:43:36 -0800 (PST)
8, 32 -- Date: Thu, 5 Mar 2009 10:43:36 -0700
8, 32 -- Message-ID: {c0bfda950903050943r4e21489avb3db134e0bae4911-at-mail.gmail.com}
8, 32 -- Subject: Regarding FEI Computers and Software
8, 32 -- From: ID {ian.drucker-at-gmail.com}
8, 32 -- To: Microscopy-at-microscopy.com
8, 32 -- Content-Type: text/plain; charset=ISO-8859-1
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25HhcE1027186
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 5 Mar 2009 12:22:16 -0600
Subject: [Microscopy] EMs and computers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here's the perspective from an engineer who is in a position to know
what he's speaking about, and who shall remain unnamed. I thought this
was very informative.

"Hi Phil and Randy,

.......... let me explain what's going on. For quite a while now, there
have been calculations required to make your "turning a knob" into a
predictable and usable result in, say, the magnification. With current
microscopes, the optics model is quite intricate.
Customers (especially the ones new to electron microscopy) don't want to
"control the objective lens current" - they want to "set the
magnification". Even the old Philips CMs had computers inside ("CM"
stood for "Computerized Microscope"). So even when you don't _see_ a
computer (as in a beige box next to the microscope), there certainly is
one.

Given the enormous pace at which consumer-level computer hardware is
progressing, it simply doesn't make sense to try and build your own
internal computer systems anymore. Customers expect more and more from
their systems (perhaps you personally don't, but especially where
microscopes are used for routine/QA work, people demand that they can be
automated).

The downside of all this is that consumer PC hardware has a "few years"
time scale whereas microscopes have a "several decades" time scale. At
the time, using ISA slots was a good idea because ISA "wasn't going
anywhere soon"!. Well, it went somewhere anyway, and systems which
still have ISA slots are getting rare.

Microscope manufacturers are obviously trying to minimize these risks
now. We won't jump on just any new technology lest it becomes an "also
ran" so we want a proven track record, but that means that probably half
of this technologies life span has already passed once we decide we can
safely use it. We are caught between a rock and a hard place.

But we are learning. The obvious advantages to riding the "commodity
hardware" wave cannot be ignored, and on the other hand we started to
realize that when the PC world says "this new technology/bus/OS will be
here forever" they really mean "at least until next week!". So we have
to tread carefully, and that's what we're doing.

I just wanted to let you know that we engineers don't sit around waiting
anxiously for some PC technology to go obsolete so we finally have
something to do again :-)"

Signed, Joe the Engineer.


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
14, 27 -- From TindallR-at-missouri.edu Thu Mar 5 12:22:16 2009
14, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25IMETb009662
14, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 12:22:15 -0600
14, 27 -- X-IronPort-Anti-Spam-Filtered: true
14, 27 -- X-IronPort-Anti-Spam-Result: ApoEANumr0nRauUo/2dsb2JhbADJJQEJhnyITYJXgTEG
14, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
14, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Mar 2009 12:22:11 -0600
14, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 27 -- Thu, 5 Mar 2009 12:22:11 -0600
14, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 27 -- Content-class: urn:content-classes:message
14, 27 -- MIME-Version: 1.0
14, 27 -- Content-Type: text/plain;
14, 27 -- charset="us-ascii"
14, 27 -- Subject: EMs and computers
14, 27 -- Date: Thu, 5 Mar 2009 12:22:07 -0600
14, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EC3-at-UM-XMAIL08.um.umsystem.edu}
14, 27 -- X-MS-Has-Attach:
14, 27 -- X-MS-TNEF-Correlator:
14, 27 -- Thread-Topic: EMs and computers
14, 27 -- Thread-Index: Acmdv0p3nFlf+p31Qv2GdkeNmnxEVw==
14, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
14, 27 -- To: {microscopy-at-microscopy.com}
14, 27 -- X-OriginalArrivalTime: 05 Mar 2009 18:22:11.0914 (UTC) FILETIME=[4D5EC2A0:01C99DBF]
14, 27 -- Content-Transfer-Encoding: 8bit
14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25IMETb009662
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 5 Mar 2009 13:13:35 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 4, 2009, at 1:27 PM, vray-at-partbeamsystech.com wrote:

} But it is probably easier to develop "feel" for inherently "manual"
} operations, like alignments or stigmation for example, by turning
} knobs
} (even though automated focus/stig works most of the times on most of
} the
} tools).


Dear Valery, et al.,
I advise being careful about the automated functions. Like most
things, it all depends on what you need to do, whether the
autofunctions are adequate. I wrote a piece in Microscopy Today a few
years ago now about this. I found that auto focus, in particular, has
a tendency to over-correct, and if you re-do the autofocus, the focus
value found will typically oscillate with decreasing amplitude until
it reaches a stable value. Thus, if all you need is a pretty well
focussed image--one that looks good, or one where you will set defocus
to several micrometers--autofocus works well, and it is quick,
convenient, and exposes your specimen to minimal extra radiation. On
the other hand, if you are doing single-particle cryoEM at high mag
and high resolution, for example, it is good to check by looking at a
live FFT to see whether autofocus has in fact found true focus from
which you can set a relatively small defocus. (Of course, in this
case you'll be doing this at a position offset from your specimen to
avoid radiation damage.) Furthermore, it is pretty simple to focus
and stigmate quickly with live FFT at mags of ~100 kx.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Thu Mar 5 13:13:34 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25JDX4v024920
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 13:13:34 -0600
6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id C4CF966E3D4E
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:31 -0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id C489766E206C
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:30 -0800 (PST)
6, 22 -- Message-Id: {88E1DA52-2521-4ACD-8818-674B1D3A1797-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200903042127.n24LRWSS023015-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Esem users
6, 22 -- Date: Thu, 5 Mar 2009 11:13:30 -0800
6, 22 -- References: {200903042127.n24LRWSS023015-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 5 Mar 2009 14:18:17 -0600
Subject: [Microscopy] Re: EMs and computers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here is another perspective.

I think that there should be a separation in which the microscope has
"embedded control" (whether or not it is truly embedded or an external
"box") that controls the microscope functions. This box should not be a
concern to the purchaser/user of the microscope, and could be upgraded
as needed by the manufacturer to properly do the job that it was
originally delivered to do, or provide any enhancements possible. It
should provide basic control functions and accept external commands for
microscope control; it would interface to actual knobs or external
digital interfaces that it is documented to support - there are many
that could be used. It should be extremely reliable, and the
manufacturers can't blame the OS since we don't care what is inside
their box as long as it works. The "box" in this context is part of the
microscope and the manufacturer should be obligated to maintain it's
functionality for a stated period; I believe that JEOL has a policy of
maintaining a machine as long as it is kept under contract and they have
done an admirable job on our older scopes.

The client side computer can then be whatever the client requires and
can be updated as needed for new storage, graphics, networks, etc and
either the manufacturer, second sources, or open sources can provide the
control program. Purchasers drive the market and could insist on open
standards. In my opinion, government funds should not be allowed to be
spent on equipment lacking complete documentation. Manufacturers will
say - "if we did that all hell would break loose", but that is just
cover; based on what we see on this list there are plenty of issues with
the manufacturers in control. For robustness, would you choose Linux or
a popular commercial OS to control your flight to the moon? I think I
would go with Linux and well-vetted open sources :-)

Dale

TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Here's the perspective from an engineer who is in a position to know
} what he's speaking about, and who shall remain unnamed. I thought this
} was very informative.
}
} "Hi Phil and Randy,
}
} .......... let me explain what's going on. For quite a while now, there
} have been calculations required to make your "turning a knob" into a
} predictable and usable result in, say, the magnification. With current
} microscopes, the optics model is quite intricate.
} Customers (especially the ones new to electron microscopy) don't want to
} "control the objective lens current" - they want to "set the
} magnification". Even the old Philips CMs had computers inside ("CM"
} stood for "Computerized Microscope"). So even when you don't _see_ a
} computer (as in a beige box next to the microscope), there certainly is
} one.
}
} Given the enormous pace at which consumer-level computer hardware is
} progressing, it simply doesn't make sense to try and build your own
} internal computer systems anymore. Customers expect more and more from
} their systems (perhaps you personally don't, but especially where
} microscopes are used for routine/QA work, people demand that they can be
} automated).
}
} The downside of all this is that consumer PC hardware has a "few years"
} time scale whereas microscopes have a "several decades" time scale. At
} the time, using ISA slots was a good idea because ISA "wasn't going
} anywhere soon"!. Well, it went somewhere anyway, and systems which
} still have ISA slots are getting rare.
}
} Microscope manufacturers are obviously trying to minimize these risks
} now. We won't jump on just any new technology lest it becomes an "also
} ran" so we want a proven track record, but that means that probably half
} of this technologies life span has already passed once we decide we can
} safely use it. We are caught between a rock and a hard place.
}
} But we are learning. The obvious advantages to riding the "commodity
} hardware" wave cannot be ignored, and on the other hand we started to
} realize that when the PC world says "this new technology/bus/OS will be
} here forever" they really mean "at least until next week!". So we have
} to tread carefully, and that's what we're doing.
}
} I just wanted to let you know that we engineers don't sit around waiting
} anxiously for some PC technology to go obsolete so we finally have
} something to do again :-)"
}
} Signed, Joe the Engineer.
}
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original Headers==============================
} 14, 27 -- From TindallR-at-missouri.edu Thu Mar 5 12:22:16 2009
} 14, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25IMETb009662
} 14, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 12:22:15 -0600
} 14, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 14, 27 -- X-IronPort-Anti-Spam-Result: ApoEANumr0nRauUo/2dsb2JhbADJJQEJhnyITYJXgTEG
} 14, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
} 14, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Mar 2009 12:22:11 -0600
} 14, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 14, 27 -- Thu, 5 Mar 2009 12:22:11 -0600
} 14, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 14, 27 -- Content-class: urn:content-classes:message
} 14, 27 -- MIME-Version: 1.0
} 14, 27 -- Content-Type: text/plain;
} 14, 27 -- charset="us-ascii"
} 14, 27 -- Subject: EMs and computers
} 14, 27 -- Date: Thu, 5 Mar 2009 12:22:07 -0600
} 14, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EC3-at-UM-XMAIL08.um.umsystem.edu}
} 14, 27 -- X-MS-Has-Attach:
} 14, 27 -- X-MS-TNEF-Correlator:
} 14, 27 -- Thread-Topic: EMs and computers
} 14, 27 -- Thread-Index: Acmdv0p3nFlf+p31Qv2GdkeNmnxEVw==
} 14, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 14, 27 -- To: {microscopy-at-microscopy.com}
} 14, 27 -- X-OriginalArrivalTime: 05 Mar 2009 18:22:11.0914 (UTC) FILETIME=[4D5EC2A0:01C99DBF]
} 14, 27 -- Content-Transfer-Encoding: 8bit
} 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25IMETb009662
} ==============================End of - Headers==============================

==============================Original Headers==============================
5, 22 -- From dac-at-research.umass.edu Thu Mar 5 14:18:16 2009
5, 22 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25KIEtq008485
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 14:18:15 -0600
5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
5, 22 -- (authenticated bits=0)
5, 22 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n25KIANe005757
5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 15:18:11 -0500
5, 22 -- Message-ID: {49B0340E.8040609-at-research.umass.edu}
5, 22 -- Date: Thu, 05 Mar 2009 15:20:30 -0500
5, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
5, 22 -- Reply-To: dac-at-research.umass.edu
5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
5, 22 -- MIME-Version: 1.0
5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
5, 22 -- Subject: Re: [Microscopy] EMs and computers
5, 22 -- References: {200903051834.n25IY0TH018019-at-ns.microscopy.com}
5, 22 -- In-Reply-To: {200903051834.n25IY0TH018019-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Thu, 5 Mar 2009 14:50:30 -0600
Subject: [Microscopy] Re: Esem users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank

maybe not Luddites but perhaps "Grumpy Old Microscopists" - not in any
ageist way of course. But then who would we be affiliated with:
1 MSA
2 Grumpy Old Men/Women - I assume that this form of entertainment from
the likes of Rik Wakeman is available in the US and elsewhere.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: Frank_Karl-at-lincolnelectric.com

Dear Bill,

I could not agree more with your "cautionary note" - this is exactly why I
said that automated functions work well "most of the times on most of the
tools"; it should probably be added "with most of the samples" :)

As was already pointed out in this thread, when SEMs are used for routine QA
or process control, or FIBs for repetitive cutting of dozens of FA samples,
demand for push-button operation and automation is very large and justified;
in research applications flexibility and ultimate performance take priority.

My argument is that "knobs" should not be disappearing with integration of
GUI and automated functions, but remain available (at least) as
user-specifiable option, even though they are working through the
computer_we_do_not_see

Cheers,
Valery Ray

============================
www.partbeamsystech.com
PBS&T, MEO Engineering Co, Inc.
290 Broadway St., Suite 298
Methuen, MA 01844
Phone: (978) 296-5063

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Thursday, March 05, 2009 2:16 PM
To: vray-at-partbeamsystech.com


On Mar 4, 2009, at 1:27 PM, vray-at-partbeamsystech.com wrote:

} But it is probably easier to develop "feel" for inherently "manual"
} operations, like alignments or stigmation for example, by turning
} knobs
} (even though automated focus/stig works most of the times on most of
} the
} tools).


Dear Valery, et al.,
I advise being careful about the automated functions. Like most
things, it all depends on what you need to do, whether the
autofunctions are adequate. I wrote a piece in Microscopy Today a few
years ago now about this. I found that auto focus, in particular, has
a tendency to over-correct, and if you re-do the autofocus, the focus
value found will typically oscillate with decreasing amplitude until
it reaches a stable value. Thus, if all you need is a pretty well
focussed image--one that looks good, or one where you will set defocus
to several micrometers--autofocus works well, and it is quick,
convenient, and exposes your specimen to minimal extra radiation. On
the other hand, if you are doing single-particle cryoEM at high mag
and high resolution, for example, it is good to check by looking at a
live FFT to see whether autofocus has in fact found true focus from
which you can set a relatively small defocus. (Of course, in this
case you'll be doing this at a position offset from your specimen to
avoid radiation damage.) Furthermore, it is pretty simple to focus
and stigmate quickly with live FFT at mags of ~100 kx.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Thu Mar 5 13:13:34 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu
(outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n25JDX4v024920
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 13:13:34
-0600
6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id
C4CF966E3D4E
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:31
-0800 (PST)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu
[131.215.19.195])
6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id C489766E206C
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:30
-0800 (PST)
6, 22 -- Message-Id: {88E1DA52-2521-4ACD-8818-674B1D3A1797-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200903042127.n24LRWSS023015-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Esem users
6, 22 -- Date: Thu, 5 Mar 2009 11:13:30 -0800
6, 22 -- References: {200903042127.n24LRWSS023015-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================


==============================Original Headers==============================
17, 25 -- From vray-at-partbeamsystech.com Thu Mar 5 14:50:29 2009
17, 25 -- Received: from smtp104.biz.mail.re2.yahoo.com (smtp104.biz.mail.re2.yahoo.com [206.190.52.173])
17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n25KoSYN004104
17, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 14:50:29 -0600
17, 25 -- Message-Id: {200903052050.n25KoSYN004104-at-ns.microscopy.com}
17, 25 -- Received: (qmail 30519 invoked from network); 5 Mar 2009 20:50:26 -0000
17, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.67.14.77 with login)
17, 25 -- by smtp104.biz.mail.re2.yahoo.com with SMTP; 5 Mar 2009 20:50:26 -0000
17, 25 -- X-YMail-OSG: by.0uukVM1nHe0V.0FeP8H6sEYBnwwQGvoL.kJ_x3dRjSyTOXm8X6byhKQNbD3E0dsg6D24H9CuYQ404RYAEEePwTUQwUK.J671uwdBsJlr.koul4qnXlQiqXxOgsc0kwcVQc9vWyrI7u6XoRIcWbfsetFCn5EK7p4SW.LlktQyo3cPtljLlwaRtRWOSgYcCoAagUt7iHozplG7g0hEw7BMSm3PmwVV2
17, 25 -- X-Yahoo-Newman-Property: ymail-3
17, 25 -- Reply-To: {vray-at-partbeamsystech.com}
17, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com}
17, 25 -- To: {tivol-at-caltech.edu}
17, 25 -- Cc: {microscopy-at-microscopy.com}
17, 25 -- Subject: RE: [Microscopy] Re: Esem users
17, 25 -- Date: Thu, 5 Mar 2009 15:53:23 -0500
17, 25 -- Organization: PBST / MEO Engineering
17, 25 -- MIME-Version: 1.0
17, 25 -- Content-Type: text/plain;
17, 25 -- charset="us-ascii"
17, 25 -- Content-Transfer-Encoding: 7bit
17, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
17, 25 -- Thread-Index: Acmdxsa7P0PaslBaRNCiFiIe/2HKPAACPotg
17, 25 -- In-Reply-To: {200903051915.n25JFc9m026521-at-ns.microscopy.com}
17, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 5 Mar 2009 22:40:03 -0600
Subject: [Microscopy] EMs and PCs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all:

Interesting thread.

As an electrical engineer, software coder, microcircuit
designer and fixer, reverse engineerer, FA, and oh, a SEM
user/operator for about ten years, here are my 5mS
neuron works and 15 minutes of carpal tunnel typing.

I had three different flavors of Amray SEMs, starting with
their 1610T, then their 1830 and lastly, their 1910FE.
These were really reliable workhorses, IMO. The 1610T
had a lot of knobs. The others had a few knobs and an
assortment of buttons. The knobs and buttons were read
by an embedded microcontroller (MCU). On some boards, each
one had a MCU. In addition, the later models had multiple
A/D and D/A converters which were controlled by an MCU.
Thus, a knob on the panel is actually a potentiometer (pot)
that is converted to digital via an A/D converter. Then,
the MCU makes computations to do what the operator wants
and sends new control bytes/commands to a D/A converter that changes
mag, stig, focus, etc. So in circa 1985, computerization
was already happening but inside the tool. KLA-Tencor killed
Amray, to their recent dismay.

So at some point in time, most all SEMs and probably TEMs
had one or more computers in the loop, but they were inside the tool
rather than desktop or outside the tool. On the later Amrays,
PC10 was the killer. This is the frame grabber and buffer that
processed the SE signal and converted it to NTSC video. I have
never seen a schematic for this board. But, as packed as it
was with ICs, it was very reliable...thankfully. The 1830 and
onwards added PC control via Nibblenet and I used Soft-Imaging
ADDA-II for frame grabbing at 640x480 pixels and direct
scan capture up to 4Kx4K. The EDAX Genesis image capture
would do over 6K pixels. Very good. All went to TIF files.

I've been involved with de-integrating an FEI Sirion SFEG to
remove the integrated EDAX EDS system. Also, dumping the SEM's
Acer PC running WinNT. As I recall, NT was written by the
author of DEC's VAX/VMS--a fabulous hardware and software
architecture. Gone but fondly remembered. NT was the first
Microsoft OS to feature virtual memory--something that Unix
always had. Plug and play came into being with WinXP (forget
about Vista, IMO). The progression from Win 3 to 95 to 95SE
to NT to XP and now Vista is a long side discussion. The
differences are subtle and unique and also of impact. This
has been pointed out in prior postings.

It was also pointed out that a common interface between
the PC and the tool's guts would be beneficial. Most
useful interfaces are SCSI. This has been a standard
for many years. It is problematic for several reasons.
One is that early ISA cards worked one way (narrow) while
newer versions (PCI) work multiple ways (SE, LVDT, et al.).
This is a hassle to set up. But once done, it should
not be an issue.

In the recent past, LEO (now Zeiss) did a nice job of PC
control of the SEM. Around 2004, Zeiss offered a "hard panel"
option. This was very good. If you like knobs and buttons
you get them along with using the mouse and a PC GUI. Their
GUI has always been very nice and easy to use. The only
standard caution is to wait for a reasonably stable version
of the GUI before adopting it. Programming is the process of putting bugs in
software. The user is the one who finds the existence of
bugs and the programmers eventually fix the bugs. Often,
the bug fixes just introduce new bugs. Perhaps that is
job security for programmers?

LEO/Zeiss failed on the implementation of 16-bit TIFF.
I finally figured this out with the help of Adobe who
was the holder of the TIFF standard at the time. Zeiss
fixed the faulty byte swap order. Thank you.

Another nice feature that Zeiss has (I don't know if this is
unique to them or not) is the ability to write versatile macros to
automate the use of virtually all functions of the SEM. This
is very handy. I recall from part of the discussion that
at least one other maker might have a similar feature. But
AFIK, the Zeiss macros are very powerful.

It is inevitable that technology is going to move forward.
New SEMs will eventually become obsolete and/or unsupported
or unsupportable. At some point, E-beam SEMs will be obsoleted
by ion beam imaging tools for true nano-scale work. Even so,
with tools becoming increasingly more complex behind the panels,
it takes longer for the tools to stabilize and be readily
usable. I don't recall that being the case for earlier generations
of SEMs or FIBs.

Sorry about the novel-length posting. Perhaps this could
be a side bar topic for discussion at M&M 2009? Wow, it
sounds like there is a lot of frustration out there. However, I
get the feeling that all of the company's service techs are
good if not great. I second this. Zeiss and FEI have been
very good. I have no experience with the others.

Final note--I only reboot Zeiss and EDAX PCs when I download Microsoft
new updates to WinXP that requires re-boot. Otherwise,
no reboot. I have Mozilla on both PCs. No big deal.

DISCLAIMER: I have no financial interest in any SEM tool
maker. I just hope that what I have will work into the future
and be supported. Zeiss says that any tool will be supported
for ten years after discontinuation of production. EDAX
support has been outstanding. Given the advancement of technology
obsolescence, I think it is going to get worse overall--and this
is not the fault of the makers. I was able to switch from Si(Li)
to SDD and do not look back. In a few years under contract,
EDAX will swap out the HP XW5000 PC for a new unit. This has
been a good PC. I would suggest to all that any new PCs are
at least RAID 1 using SATA drives.

Gary Gaugler, Ph.D.


==============================Original Headers==============================
16, 17 -- From gary-at-gaugler.com Thu Mar 5 22:40:03 2009
16, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n264e3pv029664
16, 17 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 22:40:03 -0600
16, 17 -- Message-Id: {200903060440.n264e3pv029664-at-ns.microscopy.com}
16, 17 -- Received: (qmail 11163 invoked from network); 5 Mar 2009 20:39:25 -0800
16, 17 -- Received: by simscan 1.1.0 ppid: 11160, pid: 11161, t: 0.1479s
16, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
16, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
16, 17 -- by smtp2 with SMTP; 5 Mar 2009 20:39:24 -0800
16, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
16, 17 -- Date: Thu, 05 Mar 2009 20:39:54 -0800
16, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
16, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
16, 17 -- Subject: EMs and PCs
16, 17 -- Mime-Version: 1.0
16, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: maloneyb-at-fiu.edu
Date: Fri, 6 Mar 2009 05:39:46 -0600
Subject: [Microscopy] Manual found for ISI DS130

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group - thank you to all who responded regarding the manual. We
finally found a manual stuck away hidden in a drawer. Thanks again.
Sincerely,
Barbara

==============================Original Headers==============================
1, 17 -- From maloneyb-at-fiu.edu Fri Mar 6 05:39:46 2009
1, 17 -- Received: from fmailhost04.isp.att.net (fmailhost04.isp.att.net [207.115.11.54])
1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Bdk88022395
1, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 05:39:46 -0600
1, 17 -- Received: from [10.0.0.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137])
1, 17 -- by isp.att.net (frfwmhc04) with ESMTP
1, 17 -- id {20090306113945H0400pti59e} ; Fri, 6 Mar 2009 11:39:45 +0000
1, 17 -- X-Originating-IP: [74.166.189.137]
1, 17 -- Message-ID: {49B10B81.8030808-at-fiu.edu}
1, 17 -- Date: Fri, 06 Mar 2009 06:39:45 -0500
1, 17 -- From: barbara maloney {maloneyb-at-fiu.edu}
1, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
1, 17 -- MIME-Version: 1.0
1, 17 -- To: Microscopy-at-microscopy.com
1, 17 -- Subject: Manual found for ISI DS130
1, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
1, 17 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: MARIE.CANTINO-at-uconn.edu
Date: Fri, 6 Mar 2009 08:05:17 -0600
Subject: [Microscopy] EM preservation of waxy plant cuticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am working with a student who is investigating plant cuticles, a
coating on the outermost layer of cells that consists of the polymer
cutin and waxes. When examining this feature with TEM the waxes are
often lost. From what she has read, this occurs mainly during the
resin polymerization stage. The waxes typically melt at 52-56° C.
Currently she is using Spurrs resin, which has been recommended in the
literature for the type of plants (mosses) that she is examining.

Possible approaches we are considering are:

1. Low temperature embedding using UV polymerization. We have
equipment (Leica AFS) and experience with low temp embedding in
Lowicryl HM-20 for immuno, but not for preserving waxes.

2. Microwave embedding in any resin. We have a Pella microwave with
Coldspot, but haven't done much with plant tissue so far.

3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but
would like to know whether anyone in this area (CT, MA, RI, NY) who
does and would be able to help her with a one-time examination of her
samples.

If anyone has used these or other techniques for preserving waxy
cuticles or a similar material, we would be very interested in hearing
about your experience.


Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369



==============================Original Headers==============================
10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009
10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600
10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165])
10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500
10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu}
10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu}
10, 17 -- To: microscopy-at-microscopy.com
10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2)
10, 17 -- Subject: EM preservation of waxy plant cuticles
10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500
10, 17 -- X-Mailer: Apple Mail (2.929.2)
10, 17 -- Content-Transfer-Encoding: 8bit
10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Fri, 6 Mar 2009 08:35:25 -0600
Subject: [Microscopy] EM preservation of waxy plant cuticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try the paper below. I would use the SEM to look at an air dried sample first. My motto - always start with the easiest method!

Dave

Sample preparation for scanning electron microscopy of plant surfaces-Horses
for courses
A.K. Pathan a,*, J. Bond b, R.E. Gaskin a
Micron 39 (2008) 1049-1061

-----Original Message-----
X-from: MARIE.CANTINO-at-uconn.edu [mailto:MARIE.CANTINO-at-uconn.edu]
Sent: 06 March 2009 14:13
To: David Patton

I am working with a student who is investigating plant cuticles, a
coating on the outermost layer of cells that consists of the polymer
cutin and waxes. When examining this feature with TEM the waxes are
often lost. From what she has read, this occurs mainly during the
resin polymerization stage. The waxes typically melt at 52-56° C.
Currently she is using Spurrs resin, which has been recommended in the
literature for the type of plants (mosses) that she is examining.

Possible approaches we are considering are:

1. Low temperature embedding using UV polymerization. We have
equipment (Leica AFS) and experience with low temp embedding in
Lowicryl HM-20 for immuno, but not for preserving waxes.

2. Microwave embedding in any resin. We have a Pella microwave with
Coldspot, but haven't done much with plant tissue so far.

3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but
would like to know whether anyone in this area (CT, MA, RI, NY) who
does and would be able to help her with a one-time examination of her
samples.

If anyone has used these or other techniques for preserving waxy
cuticles or a similar material, we would be very interested in hearing
about your experience.


Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369



==============================Original Headers==============================
10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009
10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600
10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165])
10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500
10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu}
10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu}
10, 17 -- To: microscopy-at-microscopy.com
10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2)
10, 17 -- Subject: EM preservation of waxy plant cuticles
10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500
10, 17 -- X-Mailer: Apple Mail (2.929.2)
10, 17 -- Content-Transfer-Encoding: 8bit
10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
23, 35 -- From David.Patton-at-uwe.ac.uk Fri Mar 6 08:35:24 2009
23, 35 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65])
23, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n26EZMgj022519
23, 35 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:35:23 -0600
23, 35 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp
23, 35 -- id 6476_0585c730_0a5c_11de_9f1e_00142221cca9;
23, 35 -- Fri, 06 Mar 2009 14:35:20 +0000
23, 35 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
23, 35 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
23, 35 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
23, 35 -- 2005)) with SMTP id {0KG300CFL9UPVS-at-mta02.uwe.ac.uk} for
23, 35 -- microscopy-at-microscopy.com; Fri, 06 Mar 2009 14:35:21 +0000 (GMT)
23, 35 -- Date: Fri, 06 Mar 2009 14:33:10 +0000
23, 35 -- From: David Patton {David.Patton-at-uwe.ac.uk}
23, 35 -- Subject: RE: [Microscopy] EM preservation of waxy plant cuticles
23, 35 -- In-reply-to: {200903061413.n26EDN05013763-at-ns.microscopy.com}
23, 35 -- To: MARIE.CANTINO-at-uconn.edu
23, 35 -- Message-id: {F247F674896BE243AD8263C5280E2BDB048C4CA7-at-egen-uwe01}
23, 35 -- MIME-version: 1.0
23, 35 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
23, 35 -- Content-type: text/plain;
23, 35 -- charset="utf-8"
23, 35 -- Content-class: urn:content-classes:message
23, 35 -- Thread-topic: [Microscopy] EM preservation of waxy plant cuticles
23, 35 -- Thread-index: AcmeZbjWsRUIl4KMR/mk7/B4NLA06AAAntIA
23, 35 -- X-MS-Has-Attach:
23, 35 -- X-MS-TNEF-Correlator:
23, 35 -- References: {200903061413.n26EDN05013763-at-ns.microscopy.com}
23, 35 -- X-NAIMIME-Disclaimer: 1
23, 35 -- X-NAIMIME-Modified: 1
23, 35 -- X-NAI-Spam-Score: 0
23, 35 -- X-NAI-Spam-Rules: 1 Rules triggered
23, 35 -- RV3225=0
23, 35 -- Content-Transfer-Encoding: 8bit
23, 35 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n26EZMgj022519
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 6 Mar 2009 13:56:00 -0600
Subject: [Microscopy] Cuticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I was wondering if anyone worked on imaging the drop size/micelles of
liquid fabric softener. I just got the quanotmix imaging capsule
system and was going to try it. Anyone use these systems with or
without a stain on fabric softeners? Any advice greatly appreciated.
Thanks.
Gordon Vrdoljak.

==============================Original Headers==============================
1, 31 -- From doc.vrdoljak-at-gmail.com Fri Mar 6 12:05:13 2009
1, 31 -- Received: from yw-out-1718.google.com (yw-out-1718.google.com [74.125.46.156])
1, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26I5D4R012009
1, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 12:05:13 -0600
1, 31 -- Received: by yw-out-1718.google.com with SMTP id 4so408581ywq.0
1, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 06 Mar 2009 10:05:12 -0800 (PST)
1, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
1, 31 -- :from:to:content-type:content-transfer-encoding;
1, 31 -- bh=kk5wwTF1DmneGllhNm85gHMyiB88AQXxOw2h18Ba4VE=;
1, 31 -- b=m39AJMPQ+6eAISFZ58AB7zCwlC6EMd9mgbHg4TBNz0Swq3wivcunazrU+pZJ6i23pr
1, 31 -- GVGG675WH3sikrI/ovaO5Yy7zz0X1gjvTQ5EYlmLWM85zjG9vWYgm7O/9CpmUMuVJDWL
1, 31 -- Y0pjVJZdOnr9jZYlcE5EiLjeQV5lbyBECxDKs=
1, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
1, 31 -- :content-transfer-encoding;
1, 31 -- b=enUDeU6VAdjIjAWUGIP+GHl1HYhpWt4ujtDh0Y6u0uuhFXBF3H0gQH4KCIz12uunO9
1, 31 -- 6qNI6r3prfye0aiD/t/Fk52GIoZnYeO9DptfviQ3l6mJP+4KtLRK4cKXlfSL/nDp7/sx
1, 31 -- bqJD8MSIwutdKMgezCKauiWzdQa/9hp+46O1U=
1, 31 -- MIME-Version: 1.0
1, 31 -- Received: by 10.142.241.10 with SMTP id o10mr1194094wfh.275.1236362712125;
1, 31 -- Fri, 06 Mar 2009 10:05:12 -0800 (PST)
1, 31 -- Date: Fri, 6 Mar 2009 10:05:12 -0800
1, 31 -- Message-ID: {fb272960903061005w300f037au7c74dd18a33df59-at-mail.gmail.com}
1, 31 -- Subject: Question about micelles in fabric softener
1, 31 -- From: Gordon Vrdoljak {doc.vrdoljak-at-gmail.com}
1, 31 -- To: Microscopy-at-microscopy.com
1, 31 -- Content-Type: text/plain; charset=ISO-8859-1
1, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From excite.compieterhenk-at-jmail.co.za Fri Mar 6 12:22:58 2009
Return-Path: {excite.compieterhenk-at-jmail.co.za}
Received: from google.com ([88.227.95.152])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26IMs7a026242
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 6 Mar 2009 12:22:57 -0600
Received: from [176.184.69.190] (HELO google.com)
by deep-mud.org; Fri, 6 Mar 2009 20:23:02 +0200

Hi,

There are some additional possibilities:

LR White can be UV polymerized; it does not even require the benzoyl
peroxide to be mixed in (and that gives it YEARS of shelf life at
4C....). A dual 4W "BLB" fluorescent unit a few inches above the samples
is good; anything equivalent will work. You don't want it to polymerize
too quickly so experiment. You can use aluminum weigh pans with a cover
of Saran, Aclar, or Cellophane (good luck finding real cellophane today)
film, or gelatine capsules - you need to exclude oxygen. Since you can
embed from EtOH the harsher acetone or propylene oxide can be avoided.

Standard epoxy resins will also UV polymerize, similar conditions as above.

Even with heated polymerization, it it accelerated by higher temps but
even 50C for longer times will work.

Depending on the resolution required, the replication of the surfaces
with dental impression materials has given excellent results from plant
surfaces.
======================================================================
A procedure for SEM of complex shoot structures applied to the
inflorescence of snapdragon (Antirrhinum)
P.B.Green and P.Linstead
Protoplasma,1990, 158:33-38.


Kerr products http://www.kerrcasting.com/ has a list of world wide
dealers on their web site.

Kerr Division of Sybron Corp
28200 Wick Rd, PO Box 455
Romulus, Mich. 48174
313-9467800

Also:
Dentsply Caulk
Dentsply International, Inc.
Milford, DE 19963-0359
1-800-LD-CAULK

This is for Reprosil, a vinyl polysiloxane impression material. It comes
in various viscosities.

Other sources:
Exacta Dental Products (http://www.exactadp.com)
3M (http://www.3m.com/market/healthcare/dental2/prod_imprintII.html)
================================================


Dale


==============================Original Headers==============================
15, 20 -- From dac-at-research.umass.edu Fri Mar 6 13:56:00 2009
15, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Ju0QK029026
15, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 13:56:00 -0600
15, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
15, 20 -- (authenticated bits=0)
15, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n26JtxMJ023013
15, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
15, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:56:00 -0500
15, 20 -- Message-ID: {49B1805C.8000100-at-research.umass.edu}
15, 20 -- Date: Fri, 06 Mar 2009 14:58:20 -0500
15, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
15, 20 -- Reply-To: dac-at-research.umass.edu
15, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
15, 20 -- MIME-Version: 1.0
15, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
15, 20 -- Subject: Cuticle
15, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
15, 20 -- Content-Transfer-Encoding: 7bit
15, 20 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 6 Mar 2009 14:05:07 -0600
Subject: [Microscopy] Cuticle (bad links....)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi -

Sorry. The link for Kerr casting seems wrong - seems they moved into
other areas. May be true of the other mfg info as well. I suggest a web
search for "Dental Impression material"...

Dale

==============================Original Headers==============================
3, 20 -- From dac-at-research.umass.edu Fri Mar 6 14:05:07 2009
3, 20 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26K55Zj010381
3, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:05:06 -0600
3, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
3, 20 -- (authenticated bits=0)
3, 20 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n26K53jP003328
3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
3, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 15:05:04 -0500
3, 20 -- Message-ID: {49B1827C.7070004-at-research.umass.edu}
3, 20 -- Date: Fri, 06 Mar 2009 15:07:24 -0500
3, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
3, 20 -- Reply-To: dac-at-research.umass.edu
3, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14
3, 20 -- MIME-Version: 1.0
3, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
3, 20 -- Subject: Cuticle (bad links....)
3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 20 -- Content-Transfer-Encoding: 7bit
3, 20 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 6 Mar 2009 14:26:58 -0600
Subject: [Microscopy] Re: Cuticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Earlier, dac-at-research.umass.edu, stated:

} LR White can be UV polymerized; it does not even require the benzoyl
} peroxide to be mixed in (and that gives it YEARS of shelf life at
} 4C....). A dual 4W "BLB" fluorescent unit a few inches above the samples
} is good; anything equivalent will work. You don't want it to polymerize
} too quickly so experiment. You can use aluminum weigh pans with a cover
} of Saran, Aclar, or Cellophane (good luck finding real cellophane today)
} film, or gelatine capsules - you need to exclude oxygen. Since you can
} embed from EtOH the harsher acetone or propylene oxide can be avoided.
}
} Standard epoxy resins will also UV polymerize, similar conditions as above.

I agree that UV polymerization can be used quite effectively with the
acrylics and Vestopal, a polyester resin.

I've not had much luck using UV to polymerize epoxy, however. Since
UV does not penetrate very deeply into osmicated specimens (50-100
micrometer), specimens have to be really thin and you need to
irradiate from as many sides as possible (or rotate the specimen).
Even then.......

If you have a detailed protocol specifically for UV polymerization of
epoxy resins, I would like to try it out.

Thanks,

JB

--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
10, 19 -- From bozzola-at-siu.edu Fri Mar 6 14:26:57 2009
10, 19 -- Received: from cstmta4.siu.edu (cstmta4.siu.edu [131.230.1.4])
10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26KQvcG024685
10, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:26:57 -0600
10, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
10, 19 -- by cstmta4.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n26KQsGF015957
10, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:26:56 -0600 (CST)
10, 19 -- Mime-Version: 1.0
10, 19 -- Message-Id: {a06240803c5d7353fdf1f-at-[131.230.177.136]}
10, 19 -- In-Reply-To: {200903061956.n26JuvVL030373-at-ns.microscopy.com}
10, 19 -- References: {200903061956.n26JuvVL030373-at-ns.microscopy.com}
10, 19 -- Date: Fri, 6 Mar 2009 14:26:54 -0600
10, 19 -- To: Microscopy-at-microscopy.com
10, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
10, 19 -- Subject: Re: [Microscopy] Cuticle
10, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
10, 19 -- X-Spam-Score: 0.00%
10, 19 -- X-MASF: 0.00%
10, 19 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: Ann-Fook.Yang-at-AGR.GC.CA
Date: Fri, 6 Mar 2009 15:05:13 -0600
Subject: [Microscopy] EM preservation of waxy plant cuticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would freeze-dry or air-dry the leaves and look at them in SEM. If you can access to an environmental SEM you may look at fresh specimens also.
Wax can cause separation of leaf tissue from adjacent resin. I believe that wax would not take up water soluble stain like UA and LC to be visible under TEM even if it is not dissolved during specimen preparation.

Ann Fook




Ann-Fook Yang,
EM Unit | Unite EM
Food Safety and Quality | Salubrité et qualité des aliments
Agriculture and Agri-Food Canada | Agriculture et Agroalimentaire Canada
Edifice K.W. Neatby Building,
960 Carling Av | 960 Boulevard Carling,
Ottawa,Ontario
K1A 0C6

ann-fook.yang-at-agr.gc.ca
Telephone | Téléphone: 613-759-1638
Facsimile | Télécopieur: 613-759-1701
Teletypewriter | Téléimprimeur 613-759-7470
Government of Canada | Gouvernement du Canada





-----Original Message-----
X-from: MARIE.CANTINO-at-uconn.edu [mailto:MARIE.CANTINO-at-uconn.edu]
Sent: Friday, March 06, 2009 9:24 AM
To: Yang, Ann-Fook

I am working with a student who is investigating plant cuticles, a
coating on the outermost layer of cells that consists of the polymer
cutin and waxes. When examining this feature with TEM the waxes are
often lost. From what she has read, this occurs mainly during the
resin polymerization stage. The waxes typically melt at 52-56° C.
Currently she is using Spurrs resin, which has been recommended in the
literature for the type of plants (mosses) that she is examining.

Possible approaches we are considering are:

1. Low temperature embedding using UV polymerization. We have
equipment (Leica AFS) and experience with low temp embedding in
Lowicryl HM-20 for immuno, but not for preserving waxes.

2. Microwave embedding in any resin. We have a Pella microwave with
Coldspot, but haven't done much with plant tissue so far.

3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but
would like to know whether anyone in this area (CT, MA, RI, NY) who
does and would be able to help her with a one-time examination of her
samples.

If anyone has used these or other techniques for preserving waxy
cuticles or a similar material, we would be very interested in hearing
about your experience.


Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369



==============================Original Headers==============================
10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009
10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600
10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165])
10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707
10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500
10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu}
10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu}
10, 17 -- To: microscopy-at-microscopy.com
10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2)
10, 17 -- Subject: EM preservation of waxy plant cuticles
10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500
10, 17 -- X-Mailer: Apple Mail (2.929.2)
10, 17 -- Content-Transfer-Encoding: 8bit
10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821
==============================End of - Headers==============================


==============================Original Headers==============================
24, 22 -- From Ann-Fook.Yang-at-AGR.GC.CA Fri Mar 6 15:05:13 2009
24, 22 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118])
24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26L5CfT006926
24, 22 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 15:05:12 -0600
24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 22 -- Content-class: urn:content-classes:message
24, 22 -- MIME-Version: 1.0
24, 22 -- Content-Type: text/plain;
24, 22 -- charset="iso-8859-1"
24, 22 -- Subject: RE: [Microscopy] EM preservation of waxy plant cuticles
24, 22 -- Date: Fri, 6 Mar 2009 16:05:07 -0500
24, 22 -- Message-ID: {773CE84BBA86484A9FEDE7741B77DDA4016173B4-at-onottaxms1.AGR.GC.CA}
24, 22 -- In-Reply-To: {200903061424.n26EOR0i022003-at-ns.microscopy.com}
24, 22 -- X-MS-Has-Attach:
24, 22 -- X-MS-TNEF-Correlator:
24, 22 -- Thread-Topic: [Microscopy] EM preservation of waxy plant cuticles
24, 22 -- Thread-Index: AcmeZ0RRxcQVYtoVQCyu6l4BEt2J9gAFuZtA
24, 22 -- From: "Yang, Ann-Fook" {Ann-Fook.Yang-at-AGR.GC.CA}
24, 22 -- To: {microscopy-at-microscopy.com}
24, 22 -- X-OriginalArrivalTime: 06 Mar 2009 21:05:07.0890 (UTC) FILETIME=[3AB81120:01C99E9F]
24, 22 -- Content-Transfer-Encoding: 8bit
24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26L5CfT006926
==============================End of - Headers==============================




From: clei-at-illinois.edu
Date: Fri, 6 Mar 2009 17:45:08 -0600
Subject: [Microscopy] viaWWW: Optimal lnes current for CM12/STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both clei-at-illinois.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: clei-at-illinois.edu
Name: changhui lei

Organization: U of Illinois at Urbana-Champaign

Title-Subject: [Filtered] Optimal lnes current for CM12/STEM

Question: Dear Colleagues,

We have an old Philiphs CM12 /STEM which has the twin objetctive
lens. Recently the alignment is bad, and probably we lost the optimal
objective lens current set by manufacture. The optimal objective lens
current is usually obtained by press "Autofocus" key.

Here I am wondering if anybody who has a simlar microscope could tell
me the optimal objective lens number.

You cna find the number by the following way:
1) Press "Autofocus"
2) On the right column of screen, press "Parameters" and you will go
to parameters pages
3) on the left column of "parameters", there is "Display Currents".
After pressing the "Display Currents", there are alots of lens
current numbers show up.

I need the object and twin lens numbers.

Thanks!

Changhui


Login Host: 130.126.103.228
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Fri Mar 6 17:45:08 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Nj66p025116
13, 11 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 17:45:07 -0600
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240803c5d765e05431-at-[206.69.208.22]}
13, 11 -- Date: Fri, 6 Mar 2009 17:45:06 -0600
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: clei-at-illinois.edu (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: Optimal lnes current for CM12/STEM
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 6 Mar 2009 17:56:53 -0600
Subject: [Microscopy] Cuticle - UV and epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Dale,

Thanks for the information. I'll give it a try.

JB

} Hi John,
}
} We've only used UV polymerization in conjunction with epoxy for very
} thin samples - single cells, or thin sections being "re-embedded"
} after resin removal and immunolabeling or histochemistry - so Os
} density was not a problem.
}
} Dale
}
}
} bozzola-at-siu.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
8, 20 -- From bozzola-at-siu.edu Fri Mar 6 17:56:53 2009
8, 20 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26NuqOk002625
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 17:56:53 -0600
8, 20 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
8, 20 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n26Numj2028595
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 17:56:49 -0600 (CST)
8, 20 -- Mime-Version: 1.0
8, 20 -- Message-Id: {a06240808c5d76836d116-at-[131.230.177.136]}
8, 20 -- In-Reply-To: {49B1B39F.4060206-at-research.umass.edu}
8, 20 -- References: {200903062030.n26KUZRr032707-at-ns.microscopy.com}
8, 20 -- {49B1B39F.4060206-at-research.umass.edu}
8, 20 -- Date: Fri, 6 Mar 2009 17:56:47 -0600
8, 20 -- To: Microscopy-at-microscopy.com
8, 20 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
8, 20 -- Subject: Re: [Microscopy] Re: Cuticle - UV and epoxy
8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 20 -- X-Spam-Score: 0.00%
8, 20 -- X-MASF: 0.00%
8, 20 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Fri, 6 Mar 2009 19:08:32 -0600
Subject: [Microscopy] Re: viaWWW: Optimal lnes current for CM12/STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Changhui,

The "AutoFocus" lens current on the CM series microscopes is set in the
column alignment procedure (on the left side of the "Alignment" page).
The procedure leads you through all the steps needed for all the lens
presets and default deflector centering.

Basically, the idea is to take your sample, set a recognizable feature
to the center of the screen and tilt. If the object moves away from the
center, bring it back with the Z adjustment. This is known as setting
the eucentric height. It sets the sample onto the rotation axis of the
stage which is a reproduceable position. You then manually focus the
image and note the focus current .

It is important to keep the sample at the eucentric position for
magnification calibration. If you focus on a sample away from the
eucentric position, the focal length of the objective lens is different
causing a variation in the magnification. You can easily wind up with a
10% magnification error.

Cheers,
Henk



clei-at-illinois.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both clei-at-illinois.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: clei-at-illinois.edu
} Name: changhui lei
}
} Organization: U of Illinois at Urbana-Champaign
}
} Title-Subject: [Filtered] Optimal lnes current for CM12/STEM
}
} Question: Dear Colleagues,
}
} We have an old Philiphs CM12 /STEM which has the twin objetctive
} lens. Recently the alignment is bad, and probably we lost the optimal
} objective lens current set by manufacture. The optimal objective lens
} current is usually obtained by press "Autofocus" key.
}
} Here I am wondering if anybody who has a simlar microscope could tell
} me the optimal objective lens number.
}
} You cna find the number by the following way:
} 1) Press "Autofocus"
} 2) On the right column of screen, press "Parameters" and you will go
} to parameters pages
} 3) on the left column of "parameters", there is "Display Currents".
} After pressing the "Display Currents", there are alots of lens
} current numbers show up.
}
} I need the object and twin lens numbers.
}
} Thanks!
}
} Changhui
}
}
} Login Host: 130.126.103.228
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 13, 11 -- From zaluzec-at-microscopy.com Fri Mar 6 17:45:08 2009
} 13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Nj66p025116
} 13, 11 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 17:45:07 -0600
} 13, 11 -- Mime-Version: 1.0
} 13, 11 -- Message-Id: {p06240803c5d765e05431-at-[206.69.208.22]}
} 13, 11 -- Date: Fri, 6 Mar 2009 17:45:06 -0600
} 13, 11 -- To: microscopy-at-microscopy.com
} 13, 11 -- From: clei-at-illinois.edu (by way of MicroscopyListserver)
} 13, 11 -- Subject: viaWWW: Optimal lnes current for CM12/STEM
} 13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
one. Lately it doesn't seem to be working."

==============================Original Headers==============================
10, 26 -- From colijn.1-at-osu.edu Fri Mar 6 19:08:32 2009
10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2718VMj022615
10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 19:08:32 -0600
10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- id {01N69ZX43S8G8XH7T6-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
10, 26 -- Fri, 06 Mar 2009 20:08:31 -0500 (EST)
10, 26 -- Received: from [192.168.1.101]
10, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- with ESMTPA id {01N69ZX3LRAO8X16KG-at-ecr6.ohio-state.edu} for
10, 26 -- microscopy-at-microscopy.com; Fri, 06 Mar 2009 20:08:30 -0500 (EST)
10, 26 -- Date: Fri, 06 Mar 2009 20:08:30 -0500
10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
10, 26 -- Subject: Re: [Microscopy] viaWWW: Optimal lnes current for CM12/STEM
10, 26 -- In-reply-to: {200903062347.n26NlZIR027359-at-ns.microscopy.com}
10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
10, 26 -- To: microscopy-at-microscopy.com
10, 26 -- Message-id: {49B1C90E.9060507-at-osu.edu}
10, 26 -- MIME-version: 1.0
10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
10, 26 -- Content-transfer-encoding: 7bit
10, 26 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
10, 26 -- References: {200903062347.n26NlZIR027359-at-ns.microscopy.com}
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Sat, 7 Mar 2009 09:12:26 -0600
Subject: [Microscopy] Nickel & SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Electroplated Nickel is ferromagnetic but, as far as I know, has no remanent
magnetic field at room temperature. When examined in a high resolution SEM
at 50-100 kX, is there any concern with this type of sample?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
3, 24 -- From donc-at-asmicro.com Sat Mar 7 09:12:26 2009
3, 24 -- Received: from smtp103.sbc.mail.re2.yahoo.com (smtp103.sbc.mail.re2.yahoo.com [68.142.229.102])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n27FCPrD008197
3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2009 09:12:26 -0600
3, 24 -- Received: (qmail 54931 invoked from network); 7 Mar 2009 15:12:25 -0000
3, 24 -- Received: from unknown (HELO asm15) (donc-at-68.51.122.238 with login)
3, 24 -- by smtp103.sbc.mail.re2.yahoo.com with SMTP; 7 Mar 2009 15:12:24 -0000
3, 24 -- X-YMail-OSG: H9Wis40VM1nuqD2nM0YWlPGoGBCmDejbdqbRr.5H4OIqjgxtx3G_xlgYbzOuhJYB9QS8tYdjAvUe7FOZ5JLohMxy612n0qGHA_T.Ml4pji.QHsPU_dmYx3ii_ci3U3jZN1rEQ2kFV4MtvP7WMVSeEotHEuig4bhuM97bQ3iNjlpb.phPE3ao1ukGekoi9_BA4Pyim4zQgBRtrEMocbWTLDzbUUvdchuMXCKXe9Ib9I3EPg--
3, 24 -- X-Yahoo-Newman-Property: ymail-3
3, 24 -- Message-ID: {C0485DDD33CB4E44ACB17EEA32D78856-at-asm15}
3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
3, 24 -- Subject: Nickel & SEM
3, 24 -- Date: Sat, 7 Mar 2009 10:12:03 -0500
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- format=flowed;
3, 24 -- charset="iso-8859-1";
3, 24 -- reply-type=original
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- X-Priority: 3
3, 24 -- X-MSMail-Priority: Normal
3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: gnord-at-mindspring.com
Date: Sun, 8 Mar 2009 13:05:32 -0500
Subject: [Microscopy] Re: viaWWW: TEM: Electron diffraction Simulation program for PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Put a magnet towards the sample. If north or south
attracts the sample, it is magnetic. If so,
using a magnetic immersion lens SEM is likely going
to be an issue. Depending on the mag and magnetic
characteristics of the sample and WD, either nothing
will happen or the column could be warped...bent.
Not a good scenario.

For FEI/Philips SFEGs, use EDS mode. Poor resolution,
poor S/N but the final lens magnetic field is off. For
electroplated metals, it seems to me that a couple of
KX ought to be enough. If not, use a LEO/Zeiss FESEM, in which
case the sample characteristics are irrelevant.

gary g.


At 07:14 AM 3/7/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Sat Mar 7 11:36:58 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n27HawRI009499
9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2009 11:36:58 -0600
9, 20 -- Message-Id: {200903071736.n27HawRI009499-at-ns.microscopy.com}
9, 20 -- Received: (qmail 23150 invoked from network); 7 Mar 2009 09:36:12 -0800
9, 20 -- Received: by simscan 1.1.0 ppid: 23145, pid: 23148, t: 0.1046s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 7 Mar 2009 09:36:12 -0800
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Sat, 07 Mar 2009 09:36:47 -0800
9, 20 -- To: donc-at-asmicro.com
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] Nickel & SEM
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200903071514.n27FEt30011166-at-ns.microscopy.com}
9, 20 -- References: {200903071514.n27FEt30011166-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================

From russoantonio1979-at-libero.it Sun Mar 8 05:49:14 2009
Return-Path: {russoantonio1979-at-libero.it}
Received: from google.com (203-59-176-214.dyn.iinet.net.au [203.59.176.214])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n28AnCB3004946
for {microscopylistserverarchive-at-microscopy.com} ; Sun, 8 Mar 2009 05:49:13 -0500
Received: from [152.67.59.182] (HELO google.com)
by moldy-darkmeat.co.uk; Sun, 8 Mar 2009 19:49:12 +0900

Look at Single Crystal by Crystal Maker {http://www.crystalmaker.com/
singlecrystal/index.html} works for both Windows and Mac.
There is a demo version.

Gordon


On Mar 4, 2009, at 8:27 PM, anita.garg-at-grc.nasa.gov wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ----------------------------------------------------------------------
} -----
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both anita.garg-at-grc.nasa.gov as well as the
} MIcroscopy Listserver
} ----------------------------------------------------------------------
} -----
}
} Email: anita.garg-at-grc.nasa.gov
} Name: Anita Garg
}
} Organization: NASA GRC
}
} Title-Subject: [Filtered] TEM: Electron diffraction Simulation
} program for PC
}
} Question: Dear Colleagues
} What is the best electron-diffraction simulation program available
} for PC these days? Earlier, the "Diffract" program used to have a Mac
} version only; is there a PC version available now?
} TIA,
} Anita
}
} Login Host: 128.156.10.80
} ----------------------------------------------------------------------
} -----
}
} ==============================Original
} Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:26:48 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n251Qk9B006802
} 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:26:48
} -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240804c5d4dac1b5b9-at-[206.69.208.22]}
} 6, 11 -- Date: Wed, 4 Mar 2009 19:26:46 -0600
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: anita.garg-at-grc.nasa.gov (by way of
} MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM: Electron diffraction Simulation
} program for PC
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
6, 25 -- From gnord-at-mindspring.com Sun Mar 8 13:05:31 2009
6, 25 -- Received: from elasmtp-curtail.atl.sa.earthlink.net (elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n28I5VOl024956
6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 8 Mar 2009 13:05:31 -0500
6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 25 -- s=dk20050327; d=mindspring.com;
6, 25 -- b=idVA3CyKxwDx0IMIcUVSNa7oacjAgjbX0fZpFAyi9EvB1im9AWf0PnvM88WxOdi5;
6, 25 -- h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Content-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originating-IP;
6, 25 -- Received: from [72.73.29.117] (helo=[192.168.254.10])
6, 25 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim 4.67)
6, 25 -- (envelope-from {gnord-at-mindspring.com} )
6, 25 -- id 1LgNNV-0000Sr-2k; Sun, 08 Mar 2009 14:05:33 -0400
6, 25 -- Mime-Version: 1.0 (Apple Message framework v753.1)
6, 25 -- In-Reply-To: {200903050127.n251RFPJ008426-at-ns.microscopy.com}
6, 25 -- References: {200903050127.n251RFPJ008426-at-ns.microscopy.com}
6, 25 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
6, 25 -- Message-Id: {9FAD7904-C72B-45A0-ABE5-910B477933F1-at-mindspring.com}
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- From: Gordon Nord {gnord-at-mindspring.com}
6, 25 -- Subject: Re: [Microscopy] viaWWW: TEM: Electron diffraction Simulation program for PC
6, 25 -- Date: Sun, 8 Mar 2009 14:05:31 -0400
6, 25 -- To: anita.garg-at-grc.nasa.gov, Microscopy {microscopy-at-microscopy.com}
6, 25 -- X-Mailer: Apple Mail (2.753.1)
6, 25 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d8886924630f8852f17313f74797233710bf5b9b91c8690ac559350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
6, 25 -- X-Originating-IP: 72.73.29.117
==============================End of - Headers==============================




From: elena.belluso-at-unito.it
Date: Mon, 9 Mar 2009 02:42:34 -0500
Subject: [Microscopy] Re: viaWWW: TEM: Electron diffraction Simulation program for PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It may be not the best, but the programme that I use, CaRIne
Crystallography, is very useful.
You can see a trial version at
http://pagespro-orange.fr/carine.crystallography/

Best,
Elena

----------------------------------------------------------------------------
-------
Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography
Dipartimento di Scienze Mineralogiche e Petrologiche
Università degli Studi di Torino
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28
e-mail: elena.belluso-at-unito.it
http://www.dsmp.unito.it
----------------------------------------------------------------------------
-------



-----Messaggio originale-----
Da: gnord-at-mindspring.com [mailto:gnord-at-mindspring.com]
Inviato: domenica 8 marzo 2009 19.06
A: elena.belluso-at-unito.it
Oggetto: [Microscopy] Re: viaWWW: TEM: Electron diffraction Simulation
program for PC




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Look at Single Crystal by Crystal Maker {http://www.crystalmaker.com/
singlecrystal/index.html} works for both Windows and Mac.
There is a demo version.

Gordon


On Mar 4, 2009, at 8:27 PM, anita.garg-at-grc.nasa.gov wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ----------------------------------------------------------------------
} -----
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both anita.garg-at-grc.nasa.gov as well as the
} MIcroscopy Listserver
} ----------------------------------------------------------------------
} -----
}
} Email: anita.garg-at-grc.nasa.gov
} Name: Anita Garg
}
} Organization: NASA GRC
}
} Title-Subject: [Filtered] TEM: Electron diffraction Simulation
} program for PC
}
} Question: Dear Colleagues
} What is the best electron-diffraction simulation program available
} for PC these days? Earlier, the "Diffract" program used to have a Mac
} version only; is there a PC version available now?
} TIA,
} Anita
}
} Login Host: 128.156.10.80
} ----------------------------------------------------------------------
} -----
}
} ==============================Original
} Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:26:48 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n251Qk9B006802
} 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:26:48
} -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240804c5d4dac1b5b9-at-[206.69.208.22]}
} 6, 11 -- Date: Wed, 4 Mar 2009 19:26:46 -0600
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: anita.garg-at-grc.nasa.gov (by way of
} MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM: Electron diffraction Simulation
} program for PC
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
6, 25 -- From gnord-at-mindspring.com Sun Mar 8 13:05:31 2009
6, 25 -- Received: from elasmtp-curtail.atl.sa.earthlink.net
(elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n28I5VOl024956
6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 8 Mar 2009 13:05:31
-0500
6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 25 -- s=dk20050327; d=mindspring.com;
6, 25 --
b=idVA3CyKxwDx0IMIcUVSNa7oacjAgjbX0fZpFAyi9EvB1im9AWf0PnvM88WxOdi5;
6, 25 --
h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Conte
nt-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originatin
g-IP;
6, 25 -- Received: from [72.73.29.117] (helo=[192.168.254.10])
6, 25 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim
4.67)
6, 25 -- (envelope-from {gnord-at-mindspring.com} )
6, 25 -- id 1LgNNV-0000Sr-2k; Sun, 08 Mar 2009 14:05:33 -0400
6, 25 -- Mime-Version: 1.0 (Apple Message framework v753.1)
6, 25 -- In-Reply-To: {200903050127.n251RFPJ008426-at-ns.microscopy.com}
6, 25 -- References: {200903050127.n251RFPJ008426-at-ns.microscopy.com}
6, 25 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes;
format=flowed
6, 25 -- Message-Id: {9FAD7904-C72B-45A0-ABE5-910B477933F1-at-mindspring.com}
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- From: Gordon Nord {gnord-at-mindspring.com}
6, 25 -- Subject: Re: [Microscopy] viaWWW: TEM: Electron diffraction
Simulation program for PC
6, 25 -- Date: Sun, 8 Mar 2009 14:05:31 -0400
6, 25 -- To: anita.garg-at-grc.nasa.gov, Microscopy {microscopy-at-microscopy.com}
6, 25 -- X-Mailer: Apple Mail (2.753.1)
6, 25 -- X-ELNK-Trace:
3df33169c923931ad4c20f6b8d69d8886924630f8852f17313f74797233710bf5b9b91c8690a
c559350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
6, 25 -- X-Originating-IP: 72.73.29.117
==============================End of - Headers==============================




==============================Original Headers==============================
19, 30 -- From elena.belluso-at-unito.it Mon Mar 9 02:42:33 2009
19, 30 -- Received: from mail-out.unito.it (opterone.unito.it [130.192.119.88])
19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n297gWUM007818
19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 02:42:33 -0500
19, 30 -- Received: (from root-at-localhost)
19, 30 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) id n297gVpg015599
19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 08:42:31 +0100
19, 30 -- Received: from BELLUSO (bellusop4.dsmp.unito.it [130.192.111.68])
19, 30 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) with ESMTP id n297gS54015553
19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 08:42:28 +0100
19, 30 -- X-Icontrol: Sent by Inrete Icontrol
19, 30 -- From: "Elena Belluso" {elena.belluso-at-unito.it}
19, 30 -- To: {Microscopy-at-microscopy.com}
19, 30 -- References: {200903081806.n28I6EU5025482-at-ns.microscopy.com}
19, 30 -- Subject: Re: viaWWW: TEM: Electron diffraction Simulation program for PC
19, 30 -- Date: Mon, 9 Mar 2009 08:42:53 +0100
19, 30 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAL5JZe2RS1VMrhZqgyhCeBDCgAAAEAAAABAVmOpMHptOtHOf6M2rQCABAAAAAA==-at-unito.it}
19, 30 -- MIME-Version: 1.0
19, 30 -- Content-Type: text/plain;
19, 30 -- charset="iso-8859-1"
19, 30 -- X-Mailer: Microsoft Office Outlook 11
19, 30 -- In-Reply-To: {200903081806.n28I6EU5025482-at-ns.microscopy.com}
19, 30 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 30 -- thread-index: AcmgGJz5RYso2LuXR8edKckwFSik9QAcTvMg
19, 30 -- Disposition-Notification-To: "Elena Belluso" {elena.belluso-at-unito.it}
19, 30 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv
19, 30 -- X-Inrete-Amavisjob-Service-Runned: 6 (n297gS54015553)
19, 30 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (n297gS54015553)
19, 30 -- Content-Transfer-Encoding: 8bit
19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n297gWUM007818
==============================End of - Headers==============================




From: ben.micklem-at-pharm.ox.ac.uk
Date: Mon, 9 Mar 2009 07:10:57 -0500
Subject: [Microscopy] Fast monochrome digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was wondering whether anyone has a high frame rate (20 to 30 fps at
full resolution) monochrome camera they can recommend for fluorescence
microscopy, with a resolution of at least 2 megapixels?

It seems there are a few around 1 megapixel, but it is hard to find
anything higher resolution.


Regards,


Ben


--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

==============================Original Headers==============================
8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009
8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29CAuE5004150
8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56 -0500
8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205])
8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004Lo-TN
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000
8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
8, 26 -- (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004xU-6Z
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000
8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk}
8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000
8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
8, 26 -- MIME-Version: 1.0
8, 26 -- To: microscopy-at-microscopy.com
8, 26 -- Subject: Fast monochrome digital camera
8, 26 -- X-Enigmail-Version: 0.95.7
8, 26 -- Content-Type: text/plain; charset=ISO-8859-1
8, 26 -- Content-Transfer-Encoding: 7bit
8, 26 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================




From: Samuel.Connell-at-STJUDE.ORG
Date: Mon, 9 Mar 2009 09:15:02 -0500
Subject: [Microscopy] Re: Fast monochrome digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ben,

Could you tell us more about your application? That would certainly help out a lot for the choice of cameras. What objective do you plan to use? Does your specimen emit a lot of photons?

You aren't likely to find a camera that can read out that many pixels at the rate you are interested in, at least not that I'm aware of.

Hopefully you either may not truly need that many pixels or that speed. Lots of biological questions don't, but there are certainly situations where one would like to push the envelope on both of those parameters as your are trying to do.

You can certainly reach that rate ~30 fps with many EMCCD cameras using the 512x512 16 um pixel array, as long as you have enough photons around. This is a likely true candidate for those rates with most biological questions. If you want to increase your pixels, then you can get 4x as many pixels with the EMCCD's at 1024x1024, but you'll likely sacrifice on speed by about 3x to ~10 fps.

Cheers,

--
Samuel A. Connell
Director of Light Microscopy
Cell & Tissue Imaging Center
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Office (901) 495-2536
Cell (901) 603-3162
samuel.connell-at-stjude.org




On 3/9/09 6:20 AM, "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk} wrote:





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I was wondering whether anyone has a high frame rate (20 to 30 fps at
full resolution) monochrome camera they can recommend for fluorescence
microscopy, with a resolution of at least 2 megapixels?

It seems there are a few around 1 megapixel, but it is hard to find
anything higher resolution.


Regards,


Ben


--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

==============================Original Headers==============================
8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009
8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29CAuE5004150
8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56 -0500
8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205])
8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004Lo-TN
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000
8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
8, 26 -- (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004xU-6Z
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000
8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk}
8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000
8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
8, 26 -- MIME-Version: 1.0
8, 26 -- To: microscopy-at-microscopy.com
8, 26 -- Subject: Fast monochrome digital camera
8, 26 -- X-Enigmail-Version: 0.95.7
8, 26 -- Content-Type: text/plain; charset=ISO-8859-1
8, 26 -- Content-Transfer-Encoding: 7bit
8, 26 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================



________________________________
Email Disclaimer: www.stjude.org/emaildisclaimer



==============================Original Headers==============================
30, 24 -- From Samuel.Connell-at-STJUDE.ORG Mon Mar 9 09:15:02 2009
30, 24 -- Received: from mgate2.stjude.org (mgate2.stjude.org [192.55.208.21])
30, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29EF2gE022419
30, 24 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 09:15:02 -0500
30, 24 -- From: "Connell, Samuel" {Samuel.Connell-at-STJUDE.ORG}
30, 24 -- To: "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk}
30, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
30, 24 -- Date: Mon, 9 Mar 2009 09:15:15 -0500
30, 24 -- Subject: Re: [Microscopy] Fast monochrome digital camera
30, 24 -- Thread-Topic: [Microscopy] Fast monochrome digital camera
30, 24 -- Thread-Index: AcmgsXQ1uCG3GDzkRjyKN+1au6UlDQAEAF2A
30, 24 -- Message-ID: {C5DA8EA0.5AEC%Samuel.Connell-at-stjude.org}
30, 24 -- In-Reply-To: {200903091220.n29CKCZ0011392-at-ns.microscopy.com}
30, 24 -- Accept-Language: en-US
30, 24 -- Content-Language: en
30, 24 -- X-MS-Has-Attach:
30, 24 -- X-MS-TNEF-Correlator:
30, 24 -- acceptlanguage: en-US
30, 24 -- Content-Type: text/plain; charset="iso-8859-1"
30, 24 -- MIME-Version: 1.0
30, 24 -- X-SEF-7853D99-ADF1-478E-8894-213D316B8FFA: 1
30, 24 -- X-SEF-Processed: 6_1_1_105__2009_03_09_09_15_16
30, 24 -- Content-Transfer-Encoding: 8bit
30, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n29EF2gE022419
==============================End of - Headers==============================




From: ben.micklem-at-pharm.ox.ac.uk
Date: Mon, 9 Mar 2009 13:32:09 -0500
Subject: [Microscopy] Re: Fast monochrome digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for your reply, Samuel, and those of other who replied directly.

The camera is to fulfill several roles, and it is leading to this hard
compromise.

The camera has to provide a live view which is used for drawing
on-screen very thin neuronal processes (axon and dendritic spines)-
frame rate is crucial for fluid focusing and movement feedback. This is
mainly currently bright-field, but may be fluorescence in the future. It
also has to function as a fluorescence camera for digital stereological,
again focusing feedback needs to have no delay at all. Ideally it would
be colour for the first use, and monochrome for the second.

Previously, these problems were overcome using a CRT module that
projects the computers display image down a drawing tube, so the finest
of axons could be drawn at the full capabilities of the optics of the
microscope. These CRT unitsare no longer produced, so we have to use
bring the microscope's image into the computer, instead of the other way
around.

I think we will have to compromise on the frame rate, because the
solutions that are available, e.g. pco.1600 (1600x1200 at 30fps) is
probably too expensive (£13,500).


Ben

--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

Connell, Samuel wrote:
} Hi Ben,
}
} Could you tell us more about your application? That would certainly help out a lot for the choice of cameras. What objective do you plan to use? Does your specimen emit a lot of photons?
}
} You aren't likely to find a camera that can read out that many pixels at the rate you are interested in, at least not that I'm aware of.
}
} Hopefully you either may not truly need that many pixels or that speed. Lots of biological questions don't, but there are certainly situations where one would like to push the envelope on both of those parameters as your are trying to do.
}
} You can certainly reach that rate ~30 fps with many EMCCD cameras using the 512x512 16 um pixel array, as long as you have enough photons around. This is a likely true candidate for those rates with most biological questions. If you want to increase your pixels, then you can get 4x as many pixels with the EMCCD's at 1024x1024, but you'll likely sacrifice on speed by about 3x to ~10 fps.
}
} Cheers,
}
} --
} Samuel A. Connell
} Director of Light Microscopy
} Cell & Tissue Imaging Center
} St. Jude Children's Research Hospital
} 262 Danny Thomas Place
} Memphis, TN 38105-3678
} Office (901) 495-2536
} Cell (901) 603-3162
} samuel.connell-at-stjude.org
}
}
}
}
} On 3/9/09 6:20 AM, "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk} wrote:
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was wondering whether anyone has a high frame rate (20 to 30 fps at
} full resolution) monochrome camera they can recommend for fluorescence
} microscopy, with a resolution of at least 2 megapixels?
}
} It seems there are a few around 1 megapixel, but it is hard to find
} anything higher resolution.
}
}
} Regards,
}
}
} Ben
}
}
} --
} Imaging Technician
} MRC Anatomical Neuropharmacology Unit, Mansfield Road,
} Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
} {http://mrcanu.pharm.ox.ac.uk/}

==============================Original Headers==============================
9, 28 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 13:32:08 2009
9, 28 -- Received: from relay1.mail.ox.ac.uk (relay1.mail.ox.ac.uk [129.67.1.165])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29IW8OX013324
9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 13:32:08 -0500
9, 28 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205])
9, 28 -- by relay1.mail.ox.ac.uk with esmtp (Exim 4.69)
9, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
9, 28 -- id 1LgkGl-0002HY-6N
9, 28 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 18:32:07 +0000
9, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
9, 28 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
9, 28 -- (Exim 4.69)
9, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
9, 28 -- id 1LgkGl-0000ZT-8t
9, 28 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 18:32:07 +0000
9, 28 -- Message-ID: {49B560A7.1060006-at-pharm.ox.ac.uk}
9, 28 -- Date: Mon, 09 Mar 2009 18:32:07 +0000
9, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
9, 28 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
9, 28 -- MIME-Version: 1.0
9, 28 -- To: microscopy-at-microscopy.com
9, 28 -- Subject: Re: [Microscopy] Fast monochrome digital camera
9, 28 -- References: {C5DA8EA0.5AEC%Samuel.Connell-at-stjude.org}
9, 28 -- In-Reply-To: {C5DA8EA0.5AEC%Samuel.Connell-at-stjude.org}
9, 28 -- X-Enigmail-Version: 0.95.7
9, 28 -- Content-Type: text/plain; charset=ISO-8859-1
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================




From: adenarollins-at-hotmail.com
Date: Mon, 9 Mar 2009 18:51:59 -0500
Subject: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both adenarollins-at-hotmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: adenarollins-at-hotmail.com
Name: Adena Rollins

Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers

Question: I am mechanically polishing an 8 layer packaged die. The
Si-C discs are like mac trucks to the approximately 40 micron layered
individual die! I have switched to diamond lapping films but I am
still having a massive amount of chip outs and cracking. Is anyone
willing to fill in a fellow polisher on the secret to obtaining a
beautiful, mirror-like finish to an extremely difficult packaged die
cross section?

Thank you in advance for any information!

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Mar 9 18:51:59 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29Npv1t004063
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 18:51:58 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5db5c050970-at-[206.69.208.22]}
6, 11 -- Date: Mon, 9 Mar 2009 18:51:56 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: adenarollins-at-hotmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Mechanical Polishing of Ultra Thin Wafers
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kjl226-at-vt.edu
Date: Mon, 9 Mar 2009 18:52:30 -0500
Subject: [Microscopy] viaWWW: Freeze Fracture machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kjl226-at-vt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kjl226-at-vt.edu
Name: Kathy J. Lowe

Organization: Vet. School at Virginia Tech

Title-Subject: [Filtered] Freeze Fracture machine

Question: My name is Kathy Lowe. I'm an Electron Microscopist at
Virginia Tech. I am trying to locate a Balzer's Freeze Fracture
instrument for one of our graduate students. He is working with cell
culture. He is growing RB4(rat brain endothelial cells) and
astrocytes on culture plate inserts.
The inserts are made of HA(mixed cellulose esters) and PCF(tissue
culture-treated Isopore).
He is interested in the blood-brain barrier and wants to visualize
the connections between the two cell types using the freeze fracture
method.
I've worked on a Balzer's 400T in the past.

Does anyone know how or where I can find and use a Freeze Fracture instrument?

Login Host: 128.173.229.180
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Mar 9 18:52:30 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29NqSa7004675
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 18:52:29 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c5db5c26111c-at-[206.69.208.22]}
7, 11 -- Date: Mon, 9 Mar 2009 18:52:27 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: kjl226-at-vt.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Freeze Fracture machine
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 9 Mar 2009 19:03:11 -0500
Subject: [Microscopy] Re: viaWWW: Mechanical Polishing of Ultra Thin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is routinely done using a FIB.

Mechanical polishing is frustrating, futile,
and lots of other adverbs.

However, depending how you have set up your
specimen for polishing, it can at times produce
useable results. The trick is to use a cover glass
between the on the top of the die. Then, the die is
hot waxed to a 90 degree stub. Then, the sandwich
is polished. Leave a bit of the sandwich sticking
out so it can be polished. I've had iffy success with
this. It depends on where the stack uses W plugs or not.
Even with W, it just depends. The other factor is the
nature of the barrier metal layer type. The silicides and
salicides also come into play.

gary g.



At 04:53 PM 3/9/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 21 -- From gary-at-gaugler.com Mon Mar 9 19:03:11 2009
11, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2A03B9m024026
11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 19:03:11 -0500
11, 21 -- Message-Id: {200903100003.n2A03B9m024026-at-ns.microscopy.com}
11, 21 -- Received: (qmail 22834 invoked from network); 9 Mar 2009 17:02:11 -0700
11, 21 -- Received: by simscan 1.1.0 ppid: 22831, pid: 22832, t: 0.1053s
11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 21 -- by smtp2 with SMTP; 9 Mar 2009 17:02:11 -0700
11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 21 -- Date: Mon, 09 Mar 2009 17:03:04 -0700
11, 21 -- To: adenarollins-at-hotmail.com
11, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 21 -- Subject: Re: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin
11, 21 -- Wafers
11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 21 -- In-Reply-To: {200903092353.n29Nrx2c008878-at-ns.microscopy.com}
11, 21 -- References: {200903092353.n29Nrx2c008878-at-ns.microscopy.com}
11, 21 -- Mime-Version: 1.0
11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Mon, 9 Mar 2009 19:40:14 -0500
Subject: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Adena;
I have seen stacked die packages that had the dies bonded with a
soft epoxy that would likely not support the inner die during polishing.
If that is causing the problem you are experiencing, ion polishing with
for example a JEOL SM-09010 Cross Section Polisher could help. However,
if there are voids between the die, you will need to fill them by vacuum
infusing epoxy.

John Mardinly,
Numonyx

-----Original Message-----
X-from: adenarollins-at-hotmail.com [mailto:adenarollins-at-hotmail.com]
Sent: Monday, March 09, 2009 5:05 PM
To: MARDINLY, A

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both adenarollins-at-hotmail.com as well as the
MIcroscopy Listserver
------------------------------------------------------------------------
---

Email: adenarollins-at-hotmail.com
Name: Adena Rollins

Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers

Question: I am mechanically polishing an 8 layer packaged die. The
Si-C discs are like mac trucks to the approximately 40 micron layered
individual die! I have switched to diamond lapping films but I am
still having a massive amount of chip outs and cracking. Is anyone
willing to fill in a fellow polisher on the secret to obtaining a
beautiful, mirror-like finish to an extremely difficult packaged die
cross section?

Thank you in advance for any information!

Login Host: 63.163.107.100
------------------------------------------------------------------------
---

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Mar 9 18:51:59 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n29Npv1t004063
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009
18:51:58 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5db5c050970-at-[206.69.208.22]}
6, 11 -- Date: Mon, 9 Mar 2009 18:51:56 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: adenarollins-at-hotmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Mechanical Polishing of Ultra Thin Wafers
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================



==============================Original Headers==============================
14, 29 -- From A.MARDINLY-at-numonyx.com Mon Mar 9 19:40:14 2009
14, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2A0eEv9013688
14, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Mar 2009 19:40:14 -0500
14, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23])
14, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 1A76C14400D;
14, 29 -- Mon, 9 Mar 2009 18:43:32 -0400 (EDT)
14, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
14, 29 -- Mon, 9 Mar 2009 20:40:13 -0400
14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 29 -- Content-class: urn:content-classes:message
14, 29 -- MIME-Version: 1.0
14, 29 -- Content-Type: text/plain;
14, 29 -- charset="us-ascii"
14, 29 -- Subject: RE: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers
14, 29 -- Date: Mon, 9 Mar 2009 20:39:37 -0400
14, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39258-at-EXDRESBENMX012.numonyx.local}
14, 29 -- In-Reply-To: {200903100004.n2A04ar5028869-at-ns.microscopy.com}
14, 29 -- X-MS-Has-Attach:
14, 29 -- X-MS-TNEF-Correlator:
14, 29 -- Thread-Topic: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers
14, 29 -- Thread-Index: AcmhE89FJtV7QLHVSXCoAY4czEcQ8QABFkFA
14, 29 -- References: {200903100004.n2A04ar5028869-at-ns.microscopy.com}
14, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
14, 29 -- To: {adenarollins-at-hotmail.com}
14, 29 -- Cc: {Microscopy-at-Microscopy.com}
14, 29 -- X-OriginalArrivalTime: 10 Mar 2009 00:40:13.0486 (UTC) FILETIME=[C64AFCE0:01C9A118]
14, 29 -- Content-Transfer-Encoding: 8bit
14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2A0eEv9013688
==============================End of - Headers==============================




From: John.Catino-at-Mineralstech.com
Date: Tue, 10 Mar 2009 09:16:43 -0500
Subject: [Microscopy] Codonics NP-1660 Printer Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Available to the best offer.

Codonics NP-1660 printer. About 6 years but has not been used for the
past year. Includes 3 boxes of 1660B-A Direct Vista paper and 2 boxes
of 1600P-A paper with color ribbon.

If interested, please respond directly to me. Please remove
[microscopy] from the subject line.

John Catino

Specialty Minerals, Inc.
MINTEQ International, Inc.
Easton, PA


**********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager.
**********************************************************************


==============================Original Headers==============================
9, 16 -- From John.Catino-at-Mineralstech.com Tue Mar 10 09:16:41 2009
9, 16 -- Received: from exprod8og105.obsmtp.com (exprod8og105.obsmtp.com [64.18.3.90])
9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2AEGbxv017808
9, 16 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Mar 2009 09:16:39 -0500
9, 16 -- Received: from source ([65.209.12.89]) by exprod8ob105.postini.com ([64.18.7.12]) with SMTP
9, 16 -- ID DSNKSbZ2QlWNlL7B8wIPMOF6hwPNr6Go491J-at-postini.com; Tue, 10 Mar 2009 07:16:40 PDT
9, 16 -- Subject: Codonics NP-1660 Printer Available
9, 16 -- To: Microscopy-at-Microscopy.com
9, 16 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
9, 16 -- Message-ID: {OF6BB0B5C9.6E93EE0C-ON85257575.004E31FD-85257575.004E69E5-at-mineralstech.com}
9, 16 -- From: John.Catino-at-Mineralstech.com
9, 16 -- Date: Tue, 10 Mar 2009 10:16:29 -0400
9, 16 -- X-MIMETrack: Serialize by Router on dpn1/Minerals_Tech(Release 6.5.4FP2|September 12, 2005) at
9, 16 -- 03/10/2009 10:16:40 AM
9, 16 -- MIME-Version: 1.0
9, 16 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: dhorne-at-interchange.ubc.ca
Date: Tue, 10 Mar 2009 16:59:46 -0500
Subject: [Microscopy] viaWWW: Emitech K1250 Parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dhorne-at-interchange.ubc.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] Emitech K1250 Parts

Question: One of the temperature sensor/control units on our Emitech
K1250 has gone belly up (the temperature sensor is kaput). We've
pulled it apart and determined that we cannot fix the multilayer
control board.

That being said, I'm looking for a spare temperature controller, specifically:

Company: Red Lion
Model T48 1/16 DIN Temperature Controller

This is the unit that came standard with the unit and would fit back in nicely.

Anyone have a junked out Emitech kicking around they might want to
part ways with, or one of these units you don't need?

Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Mar 10 16:59:46 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2ALxkFX014863
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 10 Mar 2009 16:59:46 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c5dc9335276d-at-[206.69.208.22]}
10, 11 -- Date: Tue, 10 Mar 2009 16:59:45 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Emitech K1250 Parts
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: r-holdford-at-ti.com
Date: Tue, 10 Mar 2009 17:35:52 -0500
Subject: [Microscopy] Re: viaWWW: Mechanical Polishing of Ultra Thin Wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Adena: how thick is your stack and what's the package type? And John
Mardinly is correct; if you have a softer material in contact with a
hard material, the hard material will crack and chip on the side in
contact with the soft one if the direction of polish is going that way.
Usually the polishing wheel is running CCW and your section is on the
wheel with the active side of the die facing into the turning direction,
i.e. your wheel spins to the right and the section faces to the left.
The Si die is hard but the die attach epoxy is soft, so when the
wheel/polishing material pushed past the die into the epoxy, the die had
no support and will chip. This is not usually a big problem as the
front side of the die is usually the one of interest. Since you have
SiC spacers, that's harder than anything in the rest of the package and
nearly as hard the diamond you're polishing with. You don't say if you
are using diamond lapping film or diamond suspension. I usually always
prefer diamond lapping film on a glass platen with plenty of lubricant
(usually running DI water). You definitely want to keep any SiC chips
from becoming embedded in the film or elsewhere in your package or
mounting media. I rarely pot samples up in epoxy any more. If the
package has enough structural integrity, I usually treat it like a bare
die and section using old Technology Associates' brass fixtures that
have been modified to be easier to use. If the package is not strong
enough in itself, I usually sandwich it between a piece of glass
microscope slide and a glass cover slip using EpoxyBond 110 (or M-Bond,
if you prefer). Allied High Tech and Accelerated Analysis (among
others) have fixtures of a similar type to the old Technology
Associates' ones, which are no longer available.
http://www.acceleratedanalysis.com/xs_basics.html is the URL for a nice
overview of polishing basics. The references at the end are very
informative. I've been polishing bare dice and packages for 25+ years
and I'll be happy to give any help I can.


adenarollins-at-hotmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both adenarollins-at-hotmail.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: adenarollins-at-hotmail.com
} Name: Adena Rollins
}
} Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers
}
} Question: I am mechanically polishing an 8 layer packaged die. The
} Si-C discs are like mac trucks to the approximately 40 micron layered
} individual die! I have switched to diamond lapping films but I am
} still having a massive amount of chip outs and cracking. Is anyone
} willing to fill in a fellow polisher on the secret to obtaining a
} beautiful, mirror-like finish to an extremely difficult packaged die
} cross section?
}
} Thank you in advance for any information!
}
} Login Host: 63.163.107.100
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Mon Mar 9 18:51:59 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29Npv1t004063
} 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 18:51:58 -0500
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240800c5db5c050970-at-[206.69.208.22]}
} 6, 11 -- Date: Mon, 9 Mar 2009 18:51:56 -0500
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: adenarollins-at-hotmail.com (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: Mechanical Polishing of Ultra Thin Wafers
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA
Texas Instruments, Inc.
13536 N. Central Expressway MS 940
Dallas, TX 75243
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
5, 22 -- From r-holdford-at-ti.com Tue Mar 10 17:35:51 2009
5, 22 -- Received: from comal.ext.ti.com (comal.ext.ti.com [198.47.26.152])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2AMZnLK029474
5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Mar 2009 17:35:50 -0500
5, 22 -- Received: from dlep33.itg.ti.com ([157.170.170.112])
5, 22 -- by comal.ext.ti.com (8.13.7/8.13.7) with ESMTP id n2AMZgJn019311;
5, 22 -- Tue, 10 Mar 2009 17:35:47 -0500
5, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1])
5, 22 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id n2AMZf31027964;
5, 22 -- Tue, 10 Mar 2009 17:35:42 -0500 (CDT)
5, 22 -- Message-ID: {49B6EB3D.4040002-at-ti.com}
5, 22 -- Date: Tue, 10 Mar 2009 17:35:41 -0500
5, 22 -- From: Becky Holdford {r-holdford-at-ti.com}
5, 22 -- Organization: SC Packaging Development -- FA Development
5, 22 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
5, 22 -- MIME-Version: 1.0
5, 22 -- To: adenarollins-at-hotmail.com, MSA Listserver {Microscopy-at-microscopy.com}
5, 22 -- Subject: Re: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers
5, 22 -- References: {200903092352.n29NqAO6004301-at-ns.microscopy.com}
5, 22 -- In-Reply-To: {200903092352.n29NqAO6004301-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 22 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: david.knecht-at-uconn.edu
Date: Wed, 11 Mar 2009 16:29:00 -0500
Subject: [Microscopy] TIRF setup help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yvan-at-yvanlindekens.be as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yvan-at-yvanlindekens.be
Name: Yvan

Title-Subject: [Filtered] User manual for Shandon HistoCenter 2 wanted...

Question: If you have one (or a xerox copy) to spare I would be very,
very, very obliged!

Thanks in advance!

Yvan.


Login Host: 81.243.112.232
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Mar 11 08:04:37 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BD4a9K000700
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 08:04:37 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c5dd6748dcdd-at-[206.69.208.22]}
8, 11 -- Date: Wed, 11 Mar 2009 08:04:35 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: yvan-at-yvanlindekens.be (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Shandon HistoCenter 2
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From ckjsimcwpnlvssdwod.x-at-skypipeline.com Wed Mar 11 11:20:28 2009
Return-Path: {ckjsimcwpnlvssdwod.x-at-skypipeline.com}
Received: from google.com (dsl.dynamic81215244238.ttnet.net.tr [81.215.244.238] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BGKQXR021799
for {microscopylistserverarchive-at-microscopy.com} ; Wed, 11 Mar 2009 11:20:27 -0500
Received: from [95.174.92.100] (HELO google.com)
by carefulsneeze.org; Wed, 11 Mar 2009 18:20:25 +0200

We are putting our new TIRF system through its paces (Nikon TI
motorized) and need some help from experienced users:
1. We can in theory use any of the laser lines from our laser launch
(405, 488, 563, 640) for doing TIRF. In terms of switching between
the lines, is it realistic to expect that the cubes and dichroic
mirrors will be matched in position well enough that you could switch
between cubes without having to realign the system? As things are
now, if I switch cubes in the turret, the beam position changes
significantly.

2. What is the best sample for testing TIRF? I have been using
fluorescent beads in an 8 well chamber slide so far, but I am never
100% convinced as to when I am really in TIRF mode. WIth 3um beads,
they settle rapidly and I have never seen a situation where the bottom
of the bead is sharp and the top of the bead invisible as you would
expect for TIRF. With 0.5um beads, they move so fast that they don't
seem to become sharp near the coverslip unless stuck. I am guessing
that somewhere around 1um might be ideal to see them appear and
disappear in the near coverslip position.
Presumably, when in TIRF, beads stuck to the coverslip will go into
and out of focus as you move the objective, but nothing else will come
into focus in other planes. Is that the best diagnostic?

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



==============================Original Headers==============================
5, 19 -- From david.knecht-at-uconn.edu Wed Mar 11 16:28:59 2009
5, 19 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BLSxWh031235
5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 16:28:59 -0500
5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164])
5, 19 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2BLSvl3022467
5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 17:28:57 -0400
5, 19 -- Message-Id: {9CD840A9-322A-45DF-8C92-259BEE146C2E-at-uconn.edu}
5, 19 -- From: David Knecht {david.knecht-at-uconn.edu}
5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
5, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
5, 19 -- Subject: TIRF setup help
5, 19 -- Date: Wed, 11 Mar 2009 17:28:57 -0400
5, 19 -- X-Mailer: Apple Mail (2.930.3)
5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
5, 19 -- X-UConn-MailScanner: Found to be clean
5, 19 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: cgoldsmith-at-cdc.gov
Date: Wed, 11 Mar 2009 16:39:16 -0500
Subject: [Microscopy] viaWWW: Annual meeting of the Southeastern Microscopy Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cgoldsmith-at-cdc.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cgoldsmith-at-cdc.gov
Name: Cynthia Goldsmith

Organization: Centers for Disease Control and Prevention (CDC)

Title-Subject: [Filtered] Annual meeting of the Southeastern Microscopy Society

Question: The annual meeting of the Southeastern Microscopy Society
will be held this year in Athens, GA, on May 27-29. There will be
four excellent invited speakers, including Sara Miller (Duke
University), Jay Jerome (Vanderbilt University), Wilma Lingle (Mayo
Clinic) and Yiping Zhao (University of Georgia). There will also be
student presentations and contributed talks. In addition, several
workshops will be held on Wednesday, May 27.

Please visit out website at
http://www.southeasternmicroscopy.org/index-2.html for further
information.

Come join us, your participation is key!

Cynthia Goldsmith
Secretary, SEMS


Login Host: 158.111.5.34
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Mar 11 16:39:15 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BLdEvm012701
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 16:39:15 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240804c5dddfed2419-at-[206.69.208.22]}
10, 11 -- Date: Wed, 11 Mar 2009 16:39:12 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: cgoldsmith-at-cdc.gov (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Annual meeting of the Southeastern Microscopy Society
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: SBagley-at-picr.man.ac.uk
Date: Thu, 12 Mar 2009 04:28:05 -0500
Subject: [Microscopy] nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I am looking into the possibility of having a Nitrogen gas supply near a
widefield inverted microscope so that I can present hypoxic conditions
around a well plate. The microscope is surrounded by a full Solent
Scientific environmental chamber with the well plate surrounded by a
secondary chamber than is around eight inches square.

Has anyone any experience of setting up such conditions and if so what
are the steps involved to keep your health and safety manager happy?

Many thanks

Steve




Steve Bagley
Imaging Facility
Cancer Research UK
Paterson Institute for Cancer Research
University of Manchester
Wilmslow Road
Manchester
M20 9BX
UK
--------------------------------------------------------
This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.


==============================Original Headers==============================
11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009
11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2C9S2q2012729
11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 04:28:03 -0500
11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=PMAIL01.picr.man.ac.uk)
11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD))
11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} )
11, 28 -- id 1LhhCs-000EFd-5L
11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02 +0000
11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
11, 28 -- Content-class: urn:content-classes:message
11, 28 -- MIME-Version: 1.0
11, 28 -- Content-Type: text/plain;
11, 28 -- charset="us-ascii"
11, 28 -- Subject: nitrogen supply at the microscope
11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000
11, 28 -- Message-ID: {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk}
11, 28 -- X-MS-Has-Attach:
11, 28 -- X-MS-TNEF-Correlator:
11, 28 -- Importance: normal
11, 28 -- Priority: normal
11, 28 -- Thread-Topic: nitrogen supply at the microscope
11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg
11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk}
11, 28 -- To: {Microscopy-at-microscopy.com}
11, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details.
11, 28 -- Content-Transfer-Encoding: 8bit
11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Thu, 12 Mar 2009 07:50:40 -0500
Subject: [Microscopy] Re: Nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve

There should be some sort of risk assessment (in UK law). I assume
(but I'm not familiar with the apparatus) that there could be a
potential for nitrogen venting out of the apparatus or supply lines.
If there is then one thing to look at is the potential for dropping
the overall oxygen content by 1 or 2% in the room - you will need to
check those figures. But, if it can happen, then an oxygen depletion
alarm would be very important as a first step.

Hopefully this will keep you happy too, because people do die from
oxygen depletion because there is no warning.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: SBagley-at-picr.man.ac.uk

Hi Steve,
Here's my 2 cents worth:
A number of years ago a company I worked for stored a large LN2 tank in the
TEM dark room (I don't remember why). Concern was expressed that, if spilt
while decanting, the LN2 would reduce the O2 content to non-survivable
levels and we should install an O2 alarm.

Our safety officer did a quick calculation based on the size of the tank,
size of the room and assumed all the LN2 would be instantly converted to
gas. Based on his calculations O2 levels would have drop less than 1% and
that was the end of that.

I'd start with that calculation, let your safety people calculate that out
for you...makes them feel involved. Worse case, how O2 would be replaced
If your system dumped the maximum amount of N2 into your work environment.

By the way, we did end up with a O2 sensor on an semi-enclosed loading
dock, because that's were the fill fines for LN2 were located. We lost
that argument.

Stay safe.....
Frank




SBagley-at-picr.man.
ac.uk
To
03/12/2009 05:46 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] nitrogen supply at the
SBagley-at-picr.man. microscope
ac.uk












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



Hi

I am looking into the possibility of having a Nitrogen gas supply near a
widefield inverted microscope so that I can present hypoxic conditions
around a well plate. The microscope is surrounded by a full Solent
Scientific environmental chamber with the well plate surrounded by a
secondary chamber than is around eight inches square.

Has anyone any experience of setting up such conditions and if so what
are the steps involved to keep your health and safety manager happy?

Many thanks

Steve




Steve Bagley
Imaging Facility
Cancer Research UK
Paterson Institute for Cancer Research
University of Manchester
Wilmslow Road
Manchester
M20 9BX
UK
--------------------------------------------------------
This email is confidential and intended solely for the use of the person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author and do not necessarily represent
those of the Paterson Institute for Cancer Research or the University of
Manchester. It may contain information that is privileged & confidential
within the meaning of applicable law. Accordingly any dissemination,
distribution, copying, or other use of this message, or any of its
contents, by any person other than the intended recipient may constitute a
breach of civil or criminal law and is strictly prohibited. If you are NOT
the intended recipient please contact the sender and dispose of this e-mail
as soon as possible.


==============================Original
Headers==============================
11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009
11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk
[130.88.200.94])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2C9S2q2012729
11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
04:28:03 -0500
11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248]
helo=PMAIL01.picr.man.ac.uk)
11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD))
11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} )
11, 28 -- id 1LhhCs-000EFd-5L
11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02
+0000
11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
11, 28 -- Content-class: urn:content-classes:message
11, 28 -- MIME-Version: 1.0
11, 28 -- Content-Type: text/plain;
11, 28 -- charset="us-ascii"
11, 28 -- Subject: nitrogen supply at the microscope
11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000
11, 28 -- Message-ID:
{096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk}
11, 28 -- X-MS-Has-Attach:
11, 28 -- X-MS-TNEF-Correlator:
11, 28 -- Importance: normal
11, 28 -- Priority: normal
11, 28 -- Thread-Topic: nitrogen supply at the microscope
11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg
11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk}
11, 28 -- To: {Microscopy-at-microscopy.com}
11, 28 -- X-UoM: Scanned by the University Mail System. See
http://www.itservices.manchester.ac.uk/email/filtering/information/ for
details.
11, 28 -- Content-Transfer-Encoding: 8bit
11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2C9S2q2012729
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009
32, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CAwULP002858
32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 05:58:30 -0500
32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com}
32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com
32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
32, 22 -- Message-ID: {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectric.com}
32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400
32, 22 -- From: Frank_Karl-at-lincolnelectric.com
32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM,
32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM
32, 22 -- MIME-Version: 1.0
32, 22 -- Content-Type: text/plain;
32, 22 -- charset="US-ASCII"
==============================End of - Headers==============================

From rogn-at-apol.com.tw Thu Mar 12 07:05:51 2009
Return-Path: {rogn-at-apol.com.tw}
Received: from google.com (p42005-adsau12honb6-acca.tokyo.ocn.ne.jp [220.97.197.5])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CC5kDx026700
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 12 Mar 2009 07:05:47 -0500
Received: from [111.144.76.113] (HELO google.com)
by wise-white.com; Thu, 12 Mar 2009 21:05:48 +0900
Message-ID: {00000001C92D08A448363700}
Reply-To: Richmal Winton {eunice.nicklas1012-at-gmail.com}


Good morning, dear Steve (good afternoon), dear all,

I'm wondering wether you are talking about huge amounts of liquid nitrogen in a tank (volume to be filled say 15, 40, or 200 Liters??) for cryo-applications by means of special preparation apparatusses (eg. cryomicrotome, cryo-etching etc.) or even at the EM itself (cryostage, cooling of detectors etc.).

Yes, I remember that similar calculations were done for my Cryo-tank (Dewars 10 L and 40 L) and the EM-Lab by a {safety officer} (at that time in 1981 there wasn't really any "safety officer" in our institutions but the person who did the calculations, besides UO2-Ac-radiation items was the academic physicist working in the Radiology Department).

As I understood the initial question, Steve, you are dealing with the need of creating an anoxic/hypoxic environment in a chamber/housing (environmental chamber) enclosing the whole {LM Light Microscopical} widefield inverted microscope or at least the experimental setting with the 8 x 8 inches (= 20.32 cm, cf.:
http://www.onlineconversion.com/length_common.htm )?

Correct?

If so, my 2 (Euro-)cents worth is / are:

A0) remembering that (pure) nitrogen (vapor from LN2) is heavier than "AIR" in our surroundings.

A1) nobody } normally { would think about placing a dewar or overhead storage tank above your working place.

A2) most smaller dewars (for long, mid and short term storage) are placed at the floor (say opening of the dewar device reaches height of knees or at the maximum breast height, if researcher is in a sitting position)... one exception seems to be the LN2 Cooling Dewar device for my old ZEISS EM109 (vintage 1979) which is positioned approx. 20 cm above my head when sitting in front of the micr's examination window):

The evaporation of the (approximately 1 L of) LN2 (contained in the dewar which has some sort of lid with a central pore and 90 degr. angled tube-outlet directed backwards) is low and usually mixes up very rapidly with the "normal room air", especially if the room does have at least a kind of "permanent ventilation").

Nevertheless I confess that some guests visiting the Lab and sitting WITH me at the EM (with the LN2 dewar mounted) sometimes lamented on a "sudden tiredness" they felt over the minutes sitting there) (;-((

A3) If you use liquid nitrogen from a storage tank/dewar you certainly will need and have to use (very) special equipment (flanges, fittings, hoses, reducing valves, gloves and PSA protecting from splashing, etc).

A4) even if you use (only) a e. g. 5 or 10 L LN2-storing dewar under normal conditions (i.e. storage at RT and proper/safe stand at the floor bottom) the evaporation rate into the room IMO is minimal.

BUT: You don't need to store liquid nitrogen in a tank in your working room if you experiment with an- or hypoxic conditions in a small(er) and controllable testing system (chamber).

B1) for your experimental set-up perhaps you go better with pressurized gas bottles or cylinders.
Nitrogen gas is available in several kinds of quality (for your use I recommend at least quality
} 5.0 { as we can get such here in Europe from our Lab gas-suppliers) and capacities
(from hand-hold sizes to stand-cylinders).

B2) in either case (A-B) you certainly need (on the chamber) at least bore holes for filling and venting,
controlling instruments/reduction (perhaps micro-)valves.

B3) a Venting hole/port with controlling valve should be directed (best with not too long tubing) down to the
floor.

I do have implemented such a solution for venting the EM-column sections and the inbuilt 35 mm camera receptaculum for now 29 years without any complication (BTW: the death of any fly visiting my lab-rooms unfortunately seems attributable to other causes than "nitrogen overload").
Also I have used that/similar design for -/+ controlled excluding oxygen from embedding resins or overlaying bottles/Lab flasks with oxygen-sensible contents with "nearly inert" gas (if I do't need to use Freon or freon-like substitutes) .

You perhaps should discuss such a solution also with your safety officer....maybe "nasty" questions then are limited.

Best wishes and regards,

Wolfgang MUSS
Salzburg-Austria



OR Dr. phil. Wolfgang Muss
Head of EM-Lab
Institute of Pathology, SALK (Gen. Hospital)
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively (same Lab, same address)

Paracelsus Medical Private University (PMU)
Univ.-Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {

-----------------------------------------------------------------------------------------------------------
Forthcoming in 2009:
+++2009, June 11th - June 13th: 36th Ann. SCUR Meeting (SCUR meets Florence), Host: Francesca Prignano and her team+++
We cordially invite you to participate actively in the meeting.
Visit: Next Meetings at:
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/index_ger.html

============================================================================================================




} -----Ursprüngliche Nachricht-----
} Von: Frank_Karl-at-lincolnelectric.com
} [mailto:Frank_Karl-at-lincolnelectric.com]
} Gesendet: Donnerstag, 12. März 2009 12:02
} An: Muß Wolfgang
} Betreff: [Microscopy] Re: Nitrogen supply at the microscope
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi Steve,
} Here's my 2 cents worth:
} A number of years ago a company I worked for stored a large
} LN2 tank in the TEM dark room (I don't remember why).
} Concern was expressed that, if spilt while decanting, the LN2
} would reduce the O2 content to non-survivable
} levels and we should install an O2 alarm.
}
} Our safety officer did a quick calculation based on the size
} of the tank, size of the room and assumed all the LN2 would
} be instantly converted to gas. Based on his calculations O2
} levels would have drop less than 1% and that was the end of that.
}
} I'd start with that calculation, let your safety people
} calculate that out for you...makes them feel involved. Worse
} case, how O2 would be replaced If your system dumped the
} maximum amount of N2 into your work environment.
}
} By the way, we did end up with a O2 sensor on an
} semi-enclosed loading dock, because that's were the fill
} fines for LN2 were located. We lost that argument.
}
} Stay safe.....
} Frank
}
}
}
}
}
} SBagley-at-picr.man.
}
} ac.uk
}
}
} To
} 03/12/2009 05:46
} frank_karl-at-lincolnelectric.com
} AM
} cc
}
}
}
} Subject
} Please respond to [Microscopy] nitrogen
} supply at the
} SBagley-at-picr.man. microscope
}
} ac.uk
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
} --------------------------------------------------------------
} --------------
}
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
}
}
} Hi
}
} I am looking into the possibility of having a Nitrogen gas
} supply near a
} widefield inverted microscope so that I can present hypoxic conditions
} around a well plate. The microscope is surrounded by a full Solent
} Scientific environmental chamber with the well plate surrounded by a
} secondary chamber than is around eight inches square.
}
} Has anyone any experience of setting up such conditions and if so what
} are the steps involved to keep your health and safety manager happy?
}
} Many thanks
}
} Steve
}
}
}
}
} Steve Bagley
} Imaging Facility
} Cancer Research UK
} Paterson Institute for Cancer Research
} University of Manchester
} Wilmslow Road
} Manchester
} M20 9BX
} UK
} --------------------------------------------------------
} This email is confidential and intended solely for the use of
} the person(s)
} ('the intended recipient') to whom it was addressed. Any
} views or opinions
} presented are solely those of the author and do not
} necessarily represent
} those of the Paterson Institute for Cancer Research or the
} University of
} Manchester. It may contain information that is privileged &
} confidential
} within the meaning of applicable law. Accordingly any dissemination,
} distribution, copying, or other use of this message, or any of its
} contents, by any person other than the intended recipient may
} constitute a
} breach of civil or criminal law and is strictly prohibited.
} If you are NOT
} the intended recipient please contact the sender and dispose
} of this e-mail
} as soon as possible.
}
}
} ==============================Original
} Headers==============================
} 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009
} 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk
} [130.88.200.94])
} 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n2C9S2q2012729
} 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
} 04:28:03 -0500
} 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248]
} helo=PMAIL01.picr.man.ac.uk)
} 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69
} (FreeBSD))
} 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} )
} 11, 28 -- id 1LhhCs-000EFd-5L
} 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar
} 2009 09:28:02
} +0000
} 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
} 11, 28 -- Content-class: urn:content-classes:message
} 11, 28 -- MIME-Version: 1.0
} 11, 28 -- Content-Type: text/plain;
} 11, 28 -- charset="us-ascii"
} 11, 28 -- Subject: nitrogen supply at the microscope
} 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000
} 11, 28 -- Message-ID:
} {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk}
} 11, 28 -- X-MS-Has-Attach:
} 11, 28 -- X-MS-TNEF-Correlator:
} 11, 28 -- Importance: normal
} 11, 28 -- Priority: normal
} 11, 28 -- Thread-Topic: nitrogen supply at the microscope
} 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg
} 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk}
} 11, 28 -- To: {Microscopy-at-microscopy.com}
} 11, 28 -- X-UoM: Scanned by the University Mail System. See
} http://www.itservices.manchester.ac.uk/email/filtering/informa
} tion/ for
} details.
} 11, 28 -- Content-Transfer-Encoding: 8bit
} 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n2C9S2q2012729
} ==============================End of -
} Headers==============================
}
}
} --
} *************************************************************
} Note:
} The information contained in this message may be
} privileged and confidential and protected from disclosure. If
} the reader of this message is not the intended recipient, or
} an employee or agent responsible for delivering this message
} to the intended recipient, you are hereby notified that any
} dissemination, distribution or copying of this communication
} is strictly prohibited. If you have received this
} communication in error, please notify us immediately by
} replying to the message and deleting it from your computer.
} Thank you,
} The Lincoln Electric Company
} **************************************************************
}
}
} ==============================Original
} Headers==============================
} 32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009
} 32, 22 -- Received: from lincolnelectric.com
} (smtp1.lincolnelectric.com [64.109.211.114])
} 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2CAwULP002858
} 32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar
} 2009 05:58:30 -0500
} 32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com}
} 32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
} 32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com
} 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
} 32, 22 -- Message-ID:
} {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lin
} colnelectric.com}
} 32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400
} 32, 22 -- From: Frank_Karl-at-lincolnelectric.com
} 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln
} Electric/US(Release 8.0.1|February
} 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
} 32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM,
} 32, 22 -- Itemize by Router on Notescom1/Lincoln
} Electric/US(Release 8.0.1|February
} 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
} 32, 22 -- Serialize by Router on Notescom1/Lincoln
} Electric/US(Release 8.0.1|February
} 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
} 32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM
} 32, 22 -- MIME-Version: 1.0
} 32, 22 -- Content-Type: text/plain;
} 32, 22 -- charset="US-ASCII"
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
34, 36 -- From W.Muss-at-salk.at Thu Mar 12 07:50:40 2009
34, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
34, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CCoc28028093
34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 07:50:39 -0500
34, 36 -- Received: from localhost (localhost [127.0.0.1])
34, 36 -- by hermes.salk.at (Postfix) with ESMTP id 361AAC388C
34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:50:37 +0100 (CET)
34, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
34, 36 -- Received: from hermes.salk.at ([127.0.0.1])
34, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
34, 36 -- with ESMTP id Q9KVwiahvcsB for {microscopy-at-microscopy.com} ;
34, 36 -- Thu, 12 Mar 2009 13:50:36 +0100 (CET)
34, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
34, 36 -- by hermes.salk.at (Postfix) with ESMTP id C0A72C3886
34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:50:36 +0100 (CET)
34, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
34, 36 -- Thu, 12 Mar 2009 13:50:37 +0100
34, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
34, 36 -- Content-class: urn:content-classes:message
34, 36 -- MIME-Version: 1.0
34, 36 -- Content-Type: text/plain;
34, 36 -- charset="iso-8859-1"
34, 36 -- Subject: [Microscopy] Re: Nitrogen supply at the microscope
34, 36 -- Date: Thu, 12 Mar 2009 13:50:36 +0100
34, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D08D3-at-N1RZ116.lksdom21.lks.local}
34, 36 -- In-Reply-To: {200903121101.n2CB1j1i011158-at-ns.microscopy.com}
34, 36 -- X-MS-Has-Attach:
34, 36 -- X-MS-TNEF-Correlator:
34, 36 -- Thread-Topic: [Microscopy] Re: Nitrogen supply at the microscope
34, 36 -- Thread-Index: AcmjAs5LgCkLoy6rR5WAC6QUxLC8XAAAMQgA
34, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
34, 36 -- To: {microscopy-at-microscopy.com}
34, 36 -- X-OriginalArrivalTime: 12 Mar 2009 12:50:37.0019 (UTC) FILETIME=[23FAF2B0:01C9A311]
34, 36 -- X-Scanned-By: SALK-Content-Filter
34, 36 -- Content-Transfer-Encoding: 8bit
34, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2CCoc28028093
==============================End of - Headers==============================




From: florian.dallatorre-at-mat.ethz.ch
Date: Thu, 12 Mar 2009 08:00:27 -0500
Subject: [Microscopy] viaWWW: Tenupol Electropolisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both florian.dallatorre-at-mat.ethz.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: florian.dallatorre-at-mat.ethz.ch
Name: Florian Dalla Torre

Organization: Dept. Materials, ETH Zurich, Switzerland

Title-Subject: [Filtered] Tenupol Electropolisher

Question: Hi,

Does anyone have a spare cooling coil of a Tenupol jetpolisher of
Struers? Ours broke

thanks

Florian

Login Host: 82.130.65.111
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Mar 12 08:00:26 2009
9, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CD0MEt008291
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 08:00:24 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240815c5deb7cdbd89-at-[206.69.208.22]}
9, 11 -- Date: Thu, 12 Mar 2009 08:00:20 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: florian.dallatorre-at-mat.ethz.ch (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Tenupol Electropolisher
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Thu, 12 Mar 2009 12:59:12 -0500
Subject: [Microscopy] Re: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank;
Intel actually had a fatality in Malaysia about 15 years ago due
to improper set up of a nitrogen fill station in an enclosed room, so I
think that your argument that you lost about the O2 sensor, the safety
folks did the right thing for you.

John Mardinly,
Numonyx

-----Original Message-----
X-from: Frank_Karl-at-lincolnelectric.com
[mailto:Frank_Karl-at-lincolnelectric.com]
Sent: Thursday, March 12, 2009 4:05 AM
To: MARDINLY, A

Hi Steve,
Here's my 2 cents worth:
A number of years ago a company I worked for stored a large LN2 tank in
the
TEM dark room (I don't remember why). Concern was expressed that, if
spilt
while decanting, the LN2 would reduce the O2 content to non-survivable
levels and we should install an O2 alarm.

Our safety officer did a quick calculation based on the size of the
tank,
size of the room and assumed all the LN2 would be instantly converted to
gas. Based on his calculations O2 levels would have drop less than 1%
and
that was the end of that.

I'd start with that calculation, let your safety people calculate that
out
for you...makes them feel involved. Worse case, how O2 would be
replaced
If your system dumped the maximum amount of N2 into your work
environment.

By the way, we did end up with a O2 sensor on an semi-enclosed loading
dock, because that's were the fill fines for LN2 were located. We lost
that argument.

Stay safe.....
Frank





SBagley-at-picr.man.

ac.uk


To
03/12/2009 05:46 frank_karl-at-lincolnelectric.com

AM
cc



Subject
Please respond to [Microscopy] nitrogen supply at
the
SBagley-at-picr.man. microscope

ac.uk


















------------------------------------------------------------------------
----

The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America



Hi

I am looking into the possibility of having a Nitrogen gas supply near a
widefield inverted microscope so that I can present hypoxic conditions
around a well plate. The microscope is surrounded by a full Solent
Scientific environmental chamber with the well plate surrounded by a
secondary chamber than is around eight inches square.

Has anyone any experience of setting up such conditions and if so what
are the steps involved to keep your health and safety manager happy?

Many thanks

Steve




Steve Bagley
Imaging Facility
Cancer Research UK
Paterson Institute for Cancer Research
University of Manchester
Wilmslow Road
Manchester
M20 9BX
UK
--------------------------------------------------------
This email is confidential and intended solely for the use of the
person(s)
('the intended recipient') to whom it was addressed. Any views or
opinions
presented are solely those of the author and do not necessarily
represent
those of the Paterson Institute for Cancer Research or the University of
Manchester. It may contain information that is privileged & confidential
within the meaning of applicable law. Accordingly any dissemination,
distribution, copying, or other use of this message, or any of its
contents, by any person other than the intended recipient may constitute
a
breach of civil or criminal law and is strictly prohibited. If you are
NOT
the intended recipient please contact the sender and dispose of this
e-mail
as soon as possible.


==============================Original
Headers==============================
11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009
11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk
[130.88.200.94])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2C9S2q2012729
11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
04:28:03 -0500
11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248]
helo=PMAIL01.picr.man.ac.uk)
11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD))
11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} )
11, 28 -- id 1LhhCs-000EFd-5L
11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009
09:28:02
+0000
11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
11, 28 -- Content-class: urn:content-classes:message
11, 28 -- MIME-Version: 1.0
11, 28 -- Content-Type: text/plain;
11, 28 -- charset="us-ascii"
11, 28 -- Subject: nitrogen supply at the microscope
11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000
11, 28 -- Message-ID:
{096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk}
11, 28 -- X-MS-Has-Attach:
11, 28 -- X-MS-TNEF-Correlator:
11, 28 -- Importance: normal
11, 28 -- Priority: normal
11, 28 -- Thread-Topic: nitrogen supply at the microscope
11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg
11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk}
11, 28 -- To: {Microscopy-at-microscopy.com}
11, 28 -- X-UoM: Scanned by the University Mail System. See
http://www.itservices.manchester.ac.uk/email/filtering/information/ for
details.
11, 28 -- Content-Transfer-Encoding: 8bit
11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2C9S2q2012729
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original
Headers==============================
32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009
32, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com
[64.109.211.114])
32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2CAwULP002858
32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
05:58:30 -0500
32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com}
32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com
32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
32, 22 -- Message-ID:
{OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectr
ic.com}
32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400
32, 22 -- From: Frank_Karl-at-lincolnelectric.com
32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM,
32, 22 -- Itemize by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize by Router on Notescom1/Lincoln
Electric/US(Release 8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM
32, 22 -- MIME-Version: 1.0
32, 22 -- Content-Type: text/plain;
32, 22 -- charset="US-ASCII"
==============================End of -
Headers==============================



==============================Original Headers==============================
52, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 12 12:59:11 2009
52, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
52, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CHxBNq013058
52, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 12 Mar 2009 12:59:11 -0500
52, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
52, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 41DA4144040;
52, 29 -- Thu, 12 Mar 2009 12:01:57 -0400 (EDT)
52, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
52, 29 -- Thu, 12 Mar 2009 13:59:10 -0400
52, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
52, 29 -- Content-class: urn:content-classes:message
52, 29 -- MIME-Version: 1.0
52, 29 -- Content-Type: text/plain;
52, 29 -- charset="us-ascii"
52, 29 -- Subject: RE: [Microscopy] Re: nitrogen supply at the microscope
52, 29 -- Date: Thu, 12 Mar 2009 13:58:12 -0400
52, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39B68-at-EXDRESBENMX012.numonyx.local}
52, 29 -- In-Reply-To: {200903121105.n2CB5I1l021078-at-ns.microscopy.com}
52, 29 -- X-MS-Has-Attach:
52, 29 -- X-MS-TNEF-Correlator:
52, 29 -- Thread-Topic: [Microscopy] Re: nitrogen supply at the microscope
52, 29 -- Thread-Index: AcmjAnE7r4KYjsQ3SI+gyUlfeH2hwAAOU6LA
52, 29 -- References: {200903121105.n2CB5I1l021078-at-ns.microscopy.com}
52, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
52, 29 -- To: {Frank_Karl-at-lincolnelectric.com}
52, 29 -- Cc: {Microscopy-at-Microscopy.com}
52, 29 -- X-OriginalArrivalTime: 12 Mar 2009 17:59:10.0744 (UTC) FILETIME=[3F053180:01C9A33C]
52, 29 -- Content-Transfer-Encoding: 8bit
52, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2CHxBNq013058
==============================End of - Headers==============================




From: HStahlberg-at-ucdavis.edu
Date: Thu, 12 Mar 2009 13:37:25 -0500
Subject: [Microscopy] Re: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

As far as I remember, one liter of liquid nitrogen produces about 700
liters of gaseous nitrogen, which then doesn't contain any oxygen.
In addition, the nitrogen vapor is cold, and will sink to the floor.
If you experience a sudden drop in oxygen concentration, you may loose
conscience, even if the relative oxygen concentration was still within
the breathable ranges. If you then fall to the ground, you are in the
nitrogen vapor, where you will not get any oxygen. If you stay there
for 2 minutes without immediate help, you are gone. The minimum
required O2 level for survival are somewhere around 10% (?). Normal
values are 22%.

I heard the rumors of an accident, where a graduate student in Germany
tried to refill a LN2 dewar in a cold-room (low ventilation) from
another dewar one Saturday. He overfilled, the LN2 spilled onto the
floor, he lost conscience. Another graduate student saw that, tried to
reanimate his fellow in the coldroom, and both were found dead on
Monday morning.

Portable O2 meters can be found here:
http://www.ceainstr.com/pdf_datasheets/gasman2_Info.pdf
These are portable devices with a digital display of the oxygen
concentration. They are about the size of a calculator, powered by
three AA batteries. They cost $614 per device, with about 12 months of
life time of the oxygen sensor, and $110 replacement costs for the
Oxygen sensor alone.

I have no affiliation with that company what so ever, except that we
have a few of these gas meters.

Henning.




Henning Stahlberg,
Molecular & Cellular Biology, Briggs Hall 5,
University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA
Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab), Fax: +1-530-752
3085
mailto:HStahlberg-at-ucdavis.edu, Skype:henningstahlberg
http://stahlberglab.org
http://2dx.org


==============================Original Headers==============================
12, 21 -- From HStahlberg-at-ucdavis.edu Thu Mar 12 13:37:25 2009
12, 21 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CIbNtf028144
12, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:37:24 -0500
12, 21 -- Received: from [169.237.214.89] ([169.237.214.89])
12, 21 -- (authenticated bits=0)
12, 21 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n2CIbLPT009447
12, 21 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
12, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:37:21 -0700 (PDT)
12, 21 -- Message-Id: {B6B52287-A6BF-45F5-ADA7-1388684B707A-at-ucdavis.edu}
12, 21 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu}
12, 21 -- To: Microscopy-at-microscopy.com
12, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 21 -- Content-Transfer-Encoding: 7bit
12, 21 -- Mime-Version: 1.0 (Apple Message framework v930.4)
12, 21 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
12, 21 -- Date: Thu, 12 Mar 2009 11:37:21 -0700
12, 21 -- X-Mailer: Apple Mail (2.930.4)
12, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av5
12, 21 -- X-Virus-Status: Clean
12, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 12 Mar 2009 13:55:25 -0500
Subject: [Microscopy] nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 12, 2009, at 11:37 AM, HStahlberg-at-ucdavis.edu wrote:

} As far as I remember, one liter of liquid nitrogen produces about
} 700 liters of gaseous nitrogen

} The minimum required O2 level for survival are somewhere around 10%
} (?).

Dear Henning,
I think you are correct about the N2 gas/liquid ratio and too
optimistic about minimum O2. I remember a figure of 16% for survival
and 10% to keep a candle flame lit. On the other hand, people can
climb to ~6.5 km without additional O2, so if one equates the partial
pressure of O2 at that height with a percentage at sea level that
gives the same partial pressure, one can calculate an upper limit.
(The calculation will be left as an exercise for the reader. :-))
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Thu Mar 12 13:55:24 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CItNVk011150
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:55:24 -0500
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 3314F2E50B4D
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:55:21 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 45CD6328075
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:55:15 -0700 (PDT)
6, 22 -- Message-Id: {65F7E6C4-F7BA-4652-919F-39EC1F51CD17-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200903121837.n2CIbaiX028303-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope
6, 22 -- Date: Thu, 12 Mar 2009 11:55:15 -0700
6, 22 -- References: {200903121837.n2CIbaiX028303-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: rjpalmer-at-dir.nidcr.nih.gov
Date: Thu, 12 Mar 2009 15:00:30 -0500
Subject: [Microscopy] Histochoice fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would anyone care to comment on fixatives that do not contain
formaldehyde or alcohol? A commercial product of this type is known
as Histochoice MB. Pros/cons? We are interested in non-dehydrating
fixation/embedding, i.e., aqueous resins.
--
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396

==============================Original Headers==============================
1, 17 -- From rjpalmer-at-dir.nidcr.nih.gov Thu Mar 12 15:00:29 2009
1, 17 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107])
1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CK0REd027299
1, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:00:29 -0500
1, 17 -- X-IronPortListener: NIH_Relay
1, 17 -- X-SBRS: None
1, 17 -- X-IronPort-AV: E=Sophos;i="4.38,352,1233550800";
1, 17 -- d="scan'208";a="72724884"
1, 17 -- Received: from nidcr106-134.nidcr.nih.gov (HELO [128.231.106.134]) ([128.231.106.134])
1, 17 -- by nihrelayxway2.hub.nih.gov with ESMTP; 12 Mar 2009 16:00:16 -0400
1, 17 -- Mime-Version: 1.0
1, 17 -- Message-Id: {p06210200c5df19851909-at-[128.231.106.134]}
1, 17 -- Date: Thu, 12 Mar 2009 16:00:15 -0400
1, 17 -- To: Microscopy-at-microscopy.com
1, 17 -- From: "Robert J. Palmer Jr." {rjpalmer-at-dir.nidcr.nih.gov}
1, 17 -- Subject: Histochoice fixative
1, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 12 Mar 2009 15:19:11 -0500
Subject: [Microscopy] nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, this is a fine kettle of fish I got myself into.

Please pass me the salt, so I can eat my words. Several people were kind
enough to e-mail me about my error and I thank them. Their correction will
enable me to be safer. It bothers me that I trusted the GY safety people
and that I had come to depend on their evaluation. I can only wonder what
other safety related problems were missed.

thanks again

Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
6, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 15:19:10 2009
6, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CKJ9RN009192
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:19:09 -0500
6, 22 -- In-Reply-To: {200903121845.n2CIjSW2008710-at-ns.microscopy.com}
6, 22 -- Subject: nitrogen supply at the microscope
6, 22 -- To: Microscopy-at-microscopy.com
6, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
6, 22 -- Message-ID: {OF3B6AEDEA.9DD3EEC6-ON85257577.006DDBAF-85257577.006F971D-at-lincolnelectric.com}
6, 22 -- Date: Thu, 12 Mar 2009 16:18:53 -0400
6, 22 -- From: Frank_Karl-at-lincolnelectric.com
6, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM,
6, 22 -- CD-MIME complete at 03/12/2009 04:18:52 PM,
6, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM,
6, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM,
6, 22 -- Serialize complete at 03/12/2009 04:18:52 PM
6, 22 -- MIME-Version: 1.0
6, 22 -- Content-Type: text/plain;
6, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Thu, 12 Mar 2009 16:55:18 -0500
Subject: [Microscopy] Re: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You should also not discount the possibility of a pressure relief
valve failure or poor insulation causing higher than normal boil off.

I once had a tank's high pressure safety go off. Sounded like a jet
plane and it filled the room with nitrogen gas in a matter of minutes.
Called the fire department and they advised us to evacuate the room
and go outside. The came screaming up with full breathing apparat. and
checked the room for us before we went back in. No harm done, but
pretty exciting.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009
8, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2CLt5iP026014
8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 16:55:18 -0500
8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
8, 44 -- by localhost (Email Security Appliance) with SMTP id CB0691B9464_9B97F17B
8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:03 +0000 (GMT)
8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8C8E3183B0B_9B97F17F
8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:01 +0000 (GMT)
8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu, 12 Mar 2009 14:54:11 -0700
8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
8, 44 -- ESMTP id KGEXEU00.2M0 for {microscopy-at-microscopy.com} ; Thu, 12
8, 44 -- Mar 2009 14:37:42 -0700
8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1])
8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 0F9BA8F7501D
8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:11 -0700 (PDT)
8, 44 -- X-Virus-Scanned: amavisd-new at
8, 44 -- X-Spam-Flag: NO
8, 44 -- X-Spam-Score: -2.499
8, 44 -- X-Spam-Level:
8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000,
8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1]
8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
8, 44 -- with ESMTP id nPmPguQidFlT for {microscopy-at-microscopy.com} ;
8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT)
8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146])
8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2AE0A8F7501E
8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:10 -0700 (PDT)
8, 44 -- Message-Id: {21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu}
8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
8, 44 -- To: microscopy-at-microscopy.com
8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 44 -- Content-Transfer-Encoding: 7bit
8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3)
8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700
8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
8, 44 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Thu, 12 Mar 2009 17:19:23 -0500
Subject: [Microscopy] nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sometimes good intentions go awry as well. Some years ago when a freezer
or dewar failed a lab person stored items in a portable dewar and then
placed it in a cold room. It cold in there and should last
longer--right? Later someone else entered the room and nearly passed out
due to the lack of oxygen. He was smart and had experience at high
altitudes so managed to get out and survive. Whew!
Larry

jkrupp-at-deltacollege.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} You should also not discount the possibility of a pressure relief
} valve failure or poor insulation causing higher than normal boil off.
}
} I once had a tank's high pressure safety go off. Sounded like a jet
} plane and it filled the room with nitrogen gas in a matter of minutes.
} Called the fire department and they advised us to evacuate the room
} and go outside. The came screaming up with full breathing apparat. and
} checked the room for us before we went back in. No harm done, but
} pretty exciting.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original Headers==============================
} 8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009
} 8, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
} 8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2CLt5iP026014
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 16:55:18 -0500
} 8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
} 8, 44 -- by localhost (Email Security Appliance) with SMTP id CB0691B9464_9B97F17B
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:03 +0000 (GMT)
} 8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
} 8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8C8E3183B0B_9B97F17F
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:01 +0000 (GMT)
} 8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} 8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu, 12 Mar 2009 14:54:11 -0700
} 8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} 8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
} 8, 44 -- ESMTP id KGEXEU00.2M0 for {microscopy-at-microscopy.com} ; Thu, 12
} 8, 44 -- Mar 2009 14:37:42 -0700
} 8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 0F9BA8F7501D
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:11 -0700 (PDT)
} 8, 44 -- X-Virus-Scanned: amavisd-new at
} 8, 44 -- X-Spam-Flag: NO
} 8, 44 -- X-Spam-Score: -2.499
} 8, 44 -- X-Spam-Level:
} 8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000,
} 8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1]
} 8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
} 8, 44 -- with ESMTP id nPmPguQidFlT for {microscopy-at-microscopy.com} ;
} 8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT)
} 8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146])
} 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2AE0A8F7501E
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:10 -0700 (PDT)
} 8, 44 -- Message-Id: {21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu}
} 8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} 8, 44 -- To: microscopy-at-microscopy.com
} 8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
} 8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 8, 44 -- Content-Transfer-Encoding: 7bit
} 8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
} 8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700
} 8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
} 8, 44 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
6, 41 -- From Larry.Ackerman-at-ucsf.edu Thu Mar 12 17:19:22 2009
6, 41 -- Received: from emfmcb01.ucsfmedicalcenter.org (EMFMCB01.ucsfmedicalcenter.org [64.54.46.97])
6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CMJLWJ008140
6, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 17:19:22 -0500
6, 41 -- Received: from [64.54.35.209] by emfmcb01.ucsfmedicalcenter.org with
6, 41 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
6, 41 -- Thu, 12 Mar 2009 15:19:17 -0700
6, 41 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
6, 41 -- X-AuditID: 403623d1-a7451bb00000708a-90-49b98a655eb0
6, 41 -- Received: from EXHT02.net.ucsf.edu (unknown [64.54.247.219]) by
6, 41 -- vsobmcb01.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
6, 41 -- 27FF715C7 for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:19:17
6, 41 -- -0700 (PDT)
6, 41 -- Received: from EXVS05.net.ucsf.edu (64.54.128.150) by
6, 41 -- EXHT02.net.ucsf.edu (64.54.247.219) with Microsoft SMTP Server id
6, 41 -- 8.1.340.0; Thu, 12 Mar 2009 15:19:16 -0700
6, 41 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
6, 41 -- EXVS05.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar
6, 41 -- 2009 15:18:29 -0700
6, 41 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
6, 41 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar
6, 41 -- 2009 15:18:28 -0700
6, 41 -- Message-ID: {49B98A34.2010006-at-ucsf.edu}
6, 41 -- Date: Thu, 12 Mar 2009 15:18:28 -0700
6, 41 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
6, 41 -- Reply-to: larry.ackerman-at-ucsf.edu
6, 41 -- Organization: UCSF, NeuroAnatomy
6, 41 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 41 -- MIME-Version: 1.0
6, 41 -- To: Microscopy-at-microscopy.com
6, 41 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope
6, 41 -- References: {200903122203.n2CM3spi003857-at-ns.microscopy.com}
6, 41 -- In-Reply-To: {200903122203.n2CM3spi003857-at-ns.microscopy.com}
6, 41 -- X-OriginalArrivalTime: 12 Mar 2009 22:18:28.0719 (UTC)
6, 41 -- FILETIME=[784BC7F0:01C9A360]
6, 41 -- X-Brightmail-Tracker: AAAAAQ3stXE=
6, 41 -- X-WSS-ID: 65A755EF1O868741-01-01
6, 41 -- Content-Type: text/plain;
6, 41 -- charset=iso-8859-1;
6, 41 -- format=flowed
6, 41 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Thu, 12 Mar 2009 19:13:36 -0500
Subject: [Microscopy] Re: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve,

We are thinking about the setting up the same hypoxic thing here. We have
just ordered a Zeiss [PeCon] system that includes variable CO2 and a plate
cover that goes over the culture vessels to keep the 5% CO2 passing over
the top of the wells only [i.e. not filling the entire incubator
enclosure]. Sounds like you have the Solent equivalent that relies on a 5%
CO2 cylinder and coils of tubing [our Zeiss system uses 100% CO2 at
pressure via a half inch/reduction pipe and a Zeiss mechanical/electrical
controller to set the air out to whatever %CO2 you want].

Although we a way off from implementing 'hypoxia' here, no doubt we would
use a small standard regulated 100% nitrogen [no oxygen] BOC gas cylinder
to create our hypoxic air by mixing it somehow with the %CO2/air [not sure
how yet]. Back in the 1980s we set 5% CO2 levels by gas bubblers and flow
meters and I imagine we would have a similar simple mixer this time.

Given that our microscope rooms [and incubator rooms] run happily with
rather more toxic 100% CO2 supplies and with minimal concerns from our
health and safety [as they are all correctly installed and inspected
regularly], I don't envisage any problems at all installing a bijou N2 gas
cylinder [chained to the wall] - the volumes drawn off are very small [a
few cm3 per minute once purged] at the culture vessel media interface and
the air exchange rate of the air conditioning in the microscope room is
very high anyway. We wouldn't use any 'liquid nitrogen' tanks as such, so
the risk assessment would be: there isn't any really, other than those
associated with the cylinder trolley, wall clamp and a very heavy high
pressure gas cylinder. We don't have any CO2 monitors or % O2 monitors in
our cell culture incubator rooms or microscope room at present [and don't
intend to], although naturally the latter are in the rooms where our large
liquid nitrogen tissue storage tanks are.

Besides I suppose if a 1/2 inch pressurised 100% CO2 pipe ruptured in the
cell culture incubator rooms you'd get out rather than wait for any gas
monitor to warn you [the gas escape makes a very loud noise]. A large
volume of liquid nitrogen can go to gas far quicker than block of dry ice
[if you drop them], and so can be a greater hazard in a lift etc.., but
neither risks are relevant to N2 or CO2 gas cylinder hazards. I guess our
hypoxic air over the cultures would be 1% to 15% O2 [haven't thought about
%CO2].

For CO2 cylinders BOC state:
Ensure adequate ventilation. Carbon dioxide monitoring is recommended if
used or stored in a confined space.
Carbon dioxide Occupational Exposure Standard (OES):
Long Term Exposure Limit (LTEL) 5000vpm
Short Term Exposure Limit (STEL) 15000vpm

For N2 [no oxygen] BOC just say
Personal protection: Ensure adequate ventilation.

A typical small N2 cylinder used would be similar to our CO2 cylinders:
VB/LB 94x14cm net=22kg CO2=6.4kg.

N2, unlike CO2 is odourless though. Naturally I will discuss this all with
our safety officer and make a detailed N2 safety assessment though, but I
can't see it being a particular problem given the low N2 gas volumes used
[as the CO2 case is already accepted]. Perhaps I've got my cm3 N2 gas
volumes per min way out [and it's 99% N2/CO2 not 78% N2/CO2], but I don't
think so.

It would be intersting to hear how you get on.

Keith



---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3
7BN, United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com]

Sent: 12 March 2009 11:05
To: kjmorris-at-well.ox.ac.uk

Hi Steve,
Here's my 2 cents worth:
A number of years ago a company I worked for stored a large LN2 tank in
the TEM dark room (I don't remember why). Concern was expressed that, if
spilt while decanting, the LN2 would reduce the O2 content to
non-survivable levels and we should install an O2 alarm.

Our safety officer did a quick calculation based on the size of the tank,
size of the room and assumed all the LN2 would be instantly converted to
gas. Based on his calculations O2 levels would have drop less than 1% and
that was the end of that.

I'd start with that calculation, let your safety people calculate that out
for you...makes them feel involved. Worse case, how O2 would be replaced
If your system dumped the maximum amount of N2 into your work environment.

By the way, we did end up with a O2 sensor on an semi-enclosed loading
dock, because that's were the fill fines for LN2 were located. We lost
that argument.

Stay safe.....
Frank




SBagley-at-picr.man.
ac.uk
To
03/12/2009 05:46 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] nitrogen supply at the
SBagley-at-picr.man. microscope
ac.uk












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



Hi

I am looking into the possibility of having a Nitrogen gas supply near a
widefield inverted microscope so that I can present hypoxic conditions
around a well plate. The microscope is surrounded by a full Solent
Scientific environmental chamber with the well plate surrounded by a
secondary chamber than is around eight inches square.

Has anyone any experience of setting up such conditions and if so what are
the steps involved to keep your health and safety manager happy?

Many thanks

Steve




Steve Bagley
Imaging Facility
Cancer Research UK
Paterson Institute for Cancer Research
University of Manchester
Wilmslow Road
Manchester
M20 9BX
UK
--------------------------------------------------------
This email is confidential and intended solely for the use of the
person(s) ('the intended recipient') to whom it was addressed. Any views
or opinions presented are solely those of the author and do not
necessarily represent those of the Paterson Institute for Cancer Research
or the University of Manchester. It may contain information that is
privileged & confidential within the meaning of applicable law.
Accordingly any dissemination, distribution, copying, or other use of this
message, or any of its contents, by any person other than the intended
recipient may constitute a breach of civil or criminal law and is strictly
prohibited. If you are NOT the intended recipient please contact the
sender and dispose of this e-mail as soon as possible.


==============================Original
Headers==============================
11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 11, 28 --
Received: from probity.mcc.ac.uk (probity.mcc.ac.uk
[130.88.200.94])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2C9S2q2012729
11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
04:28:03 -0500
11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248]
helo=PMAIL01.picr.man.ac.uk)
11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD))
11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} )
11, 28 -- id 1LhhCs-000EFd-5L
11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02
+0000
11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 11, 28
-- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0
11, 28 -- Content-Type: text/plain;
11, 28 -- charset="us-ascii"
11, 28 -- Subject: nitrogen supply at the microscope 11, 28 -- Date: Thu,
12 Mar 2009 09:30:14 -0000 11, 28 -- Message-ID:
{096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk}
11, 28 -- X-MS-Has-Attach:
11, 28 -- X-MS-TNEF-Correlator:
11, 28 -- Importance: normal
11, 28 -- Priority: normal
11, 28 -- Thread-Topic: nitrogen supply at the microscope 11, 28 --
Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg
11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} 11, 28 -- To:
{Microscopy-at-microscopy.com} 11, 28 -- X-UoM: Scanned by the University
Mail System. See
http://www.itservices.manchester.ac.uk/email/filtering/information/ for
details.
11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted:
from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009 32,
22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com
[64.109.211.114])
32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2CAwULP002858
32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 05:58:30
-0500
32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com}
32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 32,
22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com 32, 22 --
X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 32, 22 -- Message-ID:
{OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectric.c
om}
32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400 32, 22 -- From:
Frank_Karl-at-lincolnelectric.com 32, 22 -- X-MIMETrack: CD-MIME by Router on
Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008)
at 03/12/2009 06:58:07 AM,
32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM,
32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release
8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release
8.0.1|February
32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM,
32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM
32, 22 -- MIME-Version: 1.0
32, 22 -- Content-Type: text/plain;
32, 22 -- charset="US-ASCII"
==============================End of - Headers==============================



==============================Original Headers==============================
63, 24 -- From kjmorris-at-well.ox.ac.uk Thu Mar 12 19:13:36 2009
63, 24 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
63, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2D0Damn024867
63, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 19:13:36 -0500
63, 24 -- Received: from localhost ([127.0.0.1] helo=morse.well.ox.ac.uk)
63, 24 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
63, 24 -- id 1Lhv1r-0004s1-Gn
63, 24 -- for Microscopy-at-microscopy.com; Fri, 13 Mar 2009 00:13:35 +0000
63, 24 -- Received: from 91.125.104.49
63, 24 -- (SquirrelMail authenticated user kjmorris)
63, 24 -- by morse.well.ox.ac.uk with HTTP;
63, 24 -- Fri, 13 Mar 2009 00:13:35 -0000 (GMT)
63, 24 -- Message-ID: {1808.91.125.104.49.1236903215.squirrel-at-morse.well.ox.ac.uk}
63, 24 -- Date: Fri, 13 Mar 2009 00:13:35 -0000 (GMT)
63, 24 -- Subject: RE: [Microscopy] Re: nitrogen supply at the microscope
63, 24 -- From: "Keith J Morris" {kjmorris-at-well.ox.ac.uk}
63, 24 -- To: Microscopy-at-microscopy.com
63, 24 -- Reply-To: kjmorris-at-well.ox.ac.uk
63, 24 -- User-Agent: SquirrelMail/1.4.13
63, 24 -- MIME-Version: 1.0
63, 24 -- Content-Type: text/plain;charset=iso-8859-15
63, 24 -- Content-Transfer-Encoding: 8bit
63, 24 -- X-Priority: 3 (Normal)
63, 24 -- Importance: Normal
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Thu, 12 Mar 2009 22:02:35 -0500
Subject: [Microscopy] Re: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At one of CSIRO's labs in Geelong (near Melbourne), a CSIRO staff member
died because none of the 3 safety mechanisms in the basement holding the LN2
freezers was working properly. He stepped down into the basement, passed
out immediately and that was that - this happened about 8-9 years ago.
Since then, the organisation has been extremely careful about LN2, and we
are no longer allowed to store large volumes inside. OK, it's a pain, but
you can see where they're coming from. Only takes a couple of people to
get annoyed with a beeping O2 sensor (this happens to ours when it's time to
replace the sensor) and turn it off and you've got a recipe for disaster.

Logically, all the safety stuff we have in our lab is completely over the
top, but the answer to that is - we can't allow another accident like this
one.

Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334




On 13/03/09 5:08 AM, "A.MARDINLY-at-numonyx.com" {A.MARDINLY-at-numonyx.com}
wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Frank;
} Intel actually had a fatality in Malaysia about 15 years ago due
} to improper set up of a nitrogen fill station in an enclosed room, so I
} think that your argument that you lost about the O2 sensor, the safety
} folks did the right thing for you.
}
} John Mardinly,
} Numonyx
}
} -----Original Message-----
} X-from: Frank_Karl-at-lincolnelectric.com
} [mailto:Frank_Karl-at-lincolnelectric.com]
} Sent: Thursday, March 12, 2009 4:05 AM
} To: MARDINLY, A
} Subject: [Microscopy] Re: nitrogen supply at the microscope
}
}



==============================Original Headers==============================
12, 44 -- From prvs=316e82dfa=Rosemary.White-at-csiro.au Thu Mar 12 22:02:35 2009
12, 44 -- Received: from vic-MTAout5.csiro.au (vic-MTAout5.csiro.au [150.229.64.42])
12, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2D32X8t009969
12, 44 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 22:02:34 -0500
12, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
12, 44 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
12, 44 -- s=email; t=1236913354; x=1268449354;
12, 44 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
12, 44 -- mime-version:content-transfer-encoding:content-id:
12, 44 -- content-description:resent-date:resent-from:resent-sender:
12, 44 -- resent-to:resent-cc:resent-message-id:in-reply-to:
12, 44 -- references:list-id:list-help:list-unsubscribe:
12, 44 -- list-subscribe:list-post:list-owner:list-archive;
12, 44 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
12, 44 -- |Subject:=20Re:=20[Microscopy]=20=20nitrogen=20supply=20a
12, 44 -- t=20the=20microscope|Date:=20Fri,=2013=20Mar=202009=2014:
12, 44 -- 02:28=20+1100|Message-ID:=20 {C5E017F4.42B8%rosemary.white
12, 44 -- -at-csiro.au} |To:=20 {Microscopy-at-microscopy.com}
12, 44 -- |MIME-Version:=201.0|Content-Transfer-Encoding:=207bit
12, 44 -- |In-Reply-To:=20 {200903121808.n2CI8NYg022376-at-ns.microscop
12, 44 -- y.com} ;
12, 44 -- bh=qwUDRqlFxyJkn6FLGd0FlmxHbAyoOEhMbMj3BvJ+koI=;
12, 44 -- b=ab7t6BCd13J3okWxeq62HDdqgMfCaGUfhos0yny2k6D84yTXI0T3cNjN
12, 44 -- TJO8JnVuJikl4iOTAoZPA7bJxe/85pGtsKPv/PIKlNbFlv3JE6sYuNvvJ
12, 44 -- M8pwvzhFTMYYr/W;
12, 44 -- X-IronPort-AV: E=Sophos;i="4.38,355,1233493200";
12, 44 -- d="scan'208";a="11055778"
12, 44 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
12, 44 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 13 Mar 2009 14:02:32 +1100
12, 44 -- Received: from [152.83.167.123] (152.83.167.123) by
12, 44 -- exvic-htca01.nexus.csiro.au (138.194.81.126) with Microsoft SMTP Server id
12, 44 -- 8.1.340.0; Fri, 13 Mar 2009 14:02:31 +1100
12, 44 -- User-Agent: Microsoft-Entourage/12.10.0.080409
12, 44 -- Date: Fri, 13 Mar 2009 14:02:28 +1100
12, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
12, 44 -- From: Rosemary White {rosemary.white-at-csiro.au}
12, 44 -- To: {Microscopy-at-microscopy.com}
12, 44 -- Message-ID: {C5E017F4.42B8%rosemary.white-at-csiro.au}
12, 44 -- Thread-Topic: [Microscopy] nitrogen supply at the microscope
12, 44 -- Thread-Index: AcmjPY14cDoZjeAITmu86kFx7REX9wASpcHo
12, 44 -- In-Reply-To: {200903121808.n2CI8NYg022376-at-ns.microscopy.com}
12, 44 -- MIME-Version: 1.0
12, 44 -- Content-Type: text/plain; charset="US-ASCII"
12, 44 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: yvan_lindekens-at-yahoo.com
Date: Fri, 13 Mar 2009 03:42:54 -0500
Subject: [Microscopy] Cleaning and lubricating a Jung Tetrander and a Leitz 1300 sledge microtome ???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Does anyone has information on the grease(s) used to lubricate the specimen advance (=section thickness) mechanism of a Jung Tetrander microtome? A picture of that particular model can be found here: http://www.yvanlindekens.be/microtomen/microtomen.htm

This microtome has more or less the same specimen advance mechanism as the Reichert/Jung Hn-40 sledge microtome, so I suppose the same lubricants can be applied (??) but I don't have any information on those either...

Same question on lmuibricants for the Leitz 1300.

If anyone would happen to have a user or repair manual for the Tetrander and/or the Leitz 1300 I would really appreciate a copy of it :-).
Of course I'll pay the costs.

Many thanks in advance!

Yvan.






==============================Original Headers==============================
12, 20 -- From yvan_lindekens-at-yahoo.com Fri Mar 13 03:42:53 2009
12, 20 -- Received: from web110205.mail.gq1.yahoo.com (web110205.mail.gq1.yahoo.com [67.195.8.181])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2D8grtn013718
12, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 03:42:53 -0500
12, 20 -- Received: (qmail 96143 invoked by uid 60001); 13 Mar 2009 08:42:53 -0000
12, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1236933772; bh=u3PFN5xmAeKkKwc9qU/XePpTSqt40gOyJRvzDzTYkmA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=DSLN1v/n99pjlNJQWlMys5OqOKPRikFg/9N0eAhZpBNGFPgxacNMsBUEhy/BIJnA2T2qk3vmsaiaVesLsUT3I/UGAtGXO3swIe0rx8czto8gaV/9Q0OgtR2naHQOIpSzHozoZQBYV2FYhrB1cyDN1/nuSxWnebBwFVKtFL6GAnc=
12, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 20 -- s=s1024; d=yahoo.com;
12, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 20 -- b=ppbdKNUOa8R3S4Z5mN/xrT3MnYKg+MxcyxIp5WZhJKba25k33Y0Lcm35gAr6JzISt+NXpU5O/wrnQdEPNwpt0qxK8ppYCvPD5gv5Jq33ZbLoDPzNFjRN1380Qq34a1n4GK2u/yXN3lV+9QNqiXbFHzdDYEfhD70Gszs5jpiXSKY=;
12, 20 -- Message-ID: {910041.94555.qm-at-web110205.mail.gq1.yahoo.com}
12, 20 -- X-YMail-OSG: Mpcrg.EVM1nQMDZ5tr3RBagB0jL8I9fNt9QFWV0NKIAGPvsP7PfJfbrcFdmx7pyeGFpzN1P5gYoTBBQuGawFytvwWerSoojgKaMaUU3D6_XCPQ3UETfnXXwWjZg4Xat4IWwPliFyLxi0aaQSo4Oo7FW_YUV2QGfarqJkzSA5ZI6rwVPOVni6.GfUmoSBh3IYp8TAJqEgxKlj.Ird2uJcbapDSqGpGUcxnw9tswcKSzwq0YcjxZaNAMMXGJ3D0gwrYSPP1a7lFToodNio_9EcKuzX0BGCFiev1Ba7auTSZpZ5dy4hQGeRtr1Ukcvg2ffja3IQBfovI3sRDoajp5JyUYs-
12, 20 -- Received: from [81.243.51.106] by web110205.mail.gq1.yahoo.com via HTTP; Fri, 13 Mar 2009 01:42:52 PDT
12, 20 -- X-Mailer: YahooMailWebService/0.7.289.1
12, 20 -- Date: Fri, 13 Mar 2009 01:42:52 -0700 (PDT)
12, 20 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com}
12, 20 -- Subject: Cleaning and lubricating a Jung Tetrander and a Leitz 1300 sledge microtome ???
12, 20 -- To: Microscopy-at-microscopy.com
12, 20 -- MIME-Version: 1.0
12, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Fri, 13 Mar 2009 05:28:08 -0500
Subject: [Microscopy] Fast monochrome digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill

I'm sorry but I have to take you to task about climbers at and above
6.5 km. First of all THEY MUST ACCLIMATIZE usually by walking there
and secondly a significant number of people have problems with the
reduced levels of oxygen at those altitudes. If you took the best
mountain climbers in the world and dropped them (gently) on Mount
Everest without acclimatizing they would be dead in minutes. Similarly
I think with levels of oxygen below about 16% for most people.

Perhaps if people equated a low oxygen level with the
mountaineer's "Death Zone" it would put things into perspective.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: tivol-at-caltech.edu

Samuel beat me to a reply yonks ago, but anyway:


A high frame rate low noise fluorescence camera, that's kind of asking a
lot. The Photometrics Evolve EMCCD camera has a frame rate of 30 fps at 1x
binning and is suitable for low-noise fluorescence microscopy imaging. But
even at 1x binning it's only 512x512 pixels [fast capture and zilch noise
though]. Plus don't expect much change from £25k for many EMCCD cameras. Not
sure there's anything camera wise that’s suitable for low noise fluorescence
that can take say 5MP frame rates at video frequency.

About 2 times a second [time-lapse] is the standard rate from cheaper low
noise fluorescence cameras like our [old] £8k Peltier cooled Hamamatsu Orca
100, capturing at 1xbinning ~1MP [1344x1024]. Dropping to 2xbinning will
half the pixel resolution by combining 4 pixels into 1 [with the Orca], but
it will double sensitivity [plus there is the gain control], but this won't
get you close to video rates. It depends how bright your fluorescence is and
how much you can put up with image noise or low resolution and thus poor
image quality though - megapixels aren't everything in low light situations
[just try it with your 13MP digital SLR]. Plus it depends on how much money
you have. Of course camera technology is moving at a fast pace and our
Hamamatsu Orca 100 fluorescence camera has less sensitivity and resolution
that more recent Hamamatsu type interline models [although the new cameras
will either have more sensitivity or more pixels, as an increase in one
generally reduces the other].

Spinning disk confocals also offer very fast frame rates [as they come with
expensive Photometrics 30fps EMCCD or 15fps Interline CCD type cameras or
similar modern Hamamatsu Orcas], so if you know someone friendly who has one
nearby...

Following your additional post:

Fast frame rate and bright field is no problem, get a cheap[ish] 1MP or so
video camera, there's plenty of light as you can just turn up the halogen
light bulb. Low light fluorescence, no problem, get an expensive low noise
1MP or so B&W cooled fluorescence camera. Quality hi-res bright field colour
photo images, no problem get a decent low frame rate 5MP CCD colour camera.
Total price, well a lot, from £10k upwards.

This is in fact what we did with our PALM laser dissection system, three
cameras: Zeiss Video, Zeiss MRCm [fluorescence B&W] and Zeiss MRCm [colour],
all controlled by one software package [Axiovision]. All supplied by Zeiss
though, and as part of the expensive PALM system bid [when with the generous
discount they almost came 'free']. Other Systems here generally have both
low frame rate 5MP colour CCD cameras and peltier cooled 1MP B&W
fluorescence cameras [so we do time-lapse acquisition rather than video] -
image capture/analysis software by MetaMorph, IPLabs and NIS Elements. You
can get Interline CCD colour cameras that can do binning to increase
sensitivity [like their B&W cousins] but the resolution halves with each
binning factor [never tried them - ours rely on very noisy gain for low
light, so they are no good for anything other than dazzling fluorescence -
great for transmitted light phase and stained sections though].

There are third party suppliers for those on a budget, but there's no single
ideal camera for your needs [or anybody else's probably]. If you need fast
frame rates of 30 fps, then that is the deciding factor for a camera choice.
Otherwise a low noise cooled B&W time-lapse fluorescence camera seems your
best option, as it works well for fluorescence and DIC/Ph transmitted light
and you just increase resolution by changing the objective or via a 1.5x
optical zoom [if fitted].

I've ignored things like cameras spectral sensitivity, but that can be
relevant, e.g. in respect to far-red imaging.

I expect you know all this really, but that perfect camera [also giving
change from £3,000] sadly doesn't exist.

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


-----Original Message-----
X-from: ben.micklem-at-pharm.ox.ac.uk [mailto:ben.micklem-at-pharm.ox.ac.uk]
Sent: 09 March 2009 12:24
To: kjmorris-at-well.ox.ac.uk

I was wondering whether anyone has a high frame rate (20 to 30 fps at
full resolution) monochrome camera they can recommend for fluorescence
microscopy, with a resolution of at least 2 megapixels?

It seems there are a few around 1 megapixel, but it is hard to find
anything higher resolution.


Regards,


Ben


--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

==============================Original Headers==============================
8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009
8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk
[163.1.2.169])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n29CAuE5004150
8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56
-0500
8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205])
8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004Lo-TN
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05
+0000
8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
8, 26 -- (Exim 4.69)
8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
8, 26 -- id 1LgeK1-0004xU-6Z
8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05
+0000
8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk}
8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000
8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
8, 26 -- MIME-Version: 1.0
8, 26 -- To: microscopy-at-microscopy.com
8, 26 -- Subject: Fast monochrome digital camera
8, 26 -- X-Enigmail-Version: 0.95.7
8, 26 -- Content-Type: text/plain; charset=ISO-8859-1
8, 26 -- Content-Transfer-Encoding: 7bit
8, 26 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================



==============================Original Headers==============================
29, 23 -- From kjmorris-at-well.ox.ac.uk Fri Mar 13 05:28:08 2009
29, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
29, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DAS7Fo015528
29, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 05:28:07 -0500
29, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
29, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
29, 23 -- id 1Li4cZ-0001DC-Ds
29, 23 -- for microscopy-at-microscopy.com; Fri, 13 Mar 2009 10:28:07 +0000
29, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
29, 23 -- To: {microscopy-at-microscopy.com}
29, 23 -- References: {200903091224.n29CO8nX015042-at-ns.microscopy.com}
29, 23 -- Subject: RE: [Microscopy] Fast monochrome digital camera
29, 23 -- Date: Fri, 13 Mar 2009 10:28:08 -0000
29, 23 -- Message-ID: {6070AFFE2A2244648963F54F48919BF9-at-CytoWhizz}
29, 23 -- MIME-Version: 1.0
29, 23 -- Content-Type: text/plain;
29, 23 -- charset="iso-8859-1"
29, 23 -- X-Mailer: Microsoft Office Outlook 11
29, 23 -- In-Reply-To: {200903091224.n29CO8nX015042-at-ns.microscopy.com}
29, 23 -- Thread-Index: AcmgsgN+BXsFSGZZRYKUXEWb+3xvHgBnRGsg
29, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
29, 23 -- Content-Transfer-Encoding: 8bit
29, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DAS7Fo015528
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Fri, 13 Mar 2009 10:28:52 -0500
Subject: [Microscopy] Strip Aperture holder for ISI ABT-55

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anybody have a strip aperture holder for an ABT-55 hanging
around? I have the aperture, but the holder was removed from my scope
for some reason.

Thanks,

Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
4, 31 -- From kraftpiano-at-gmail.com Fri Mar 13 10:28:51 2009
4, 31 -- Received: from mail-qy0-f119.google.com (mail-qy0-f119.google.com [209.85.221.119])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DFSote005700
4, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 10:28:51 -0500
4, 31 -- Received: by qyk17 with SMTP id 17so268135qyk.10
4, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 08:28:49 -0700 (PDT)
4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
4, 31 -- :from:to:content-type:content-transfer-encoding;
4, 31 -- bh=Bc+SfgeKimFZteVhsYksGjIvd/FJ1uWNp+kYTePy8BI=;
4, 31 -- b=JHfyFJYAz/PIqpshwWK3QXgup5+bXn2n8AS2MkdKvg0PegXqgGrcdyOWipSZCWT+Oi
4, 31 -- Yym+YsRegMQ5Ja87vmFgxBFl4mXZ7s3CYyMIUocsn5IP2+4mlQx0XZkeiAU2ZM4OWa8z
4, 31 -- nfhvOb5lZgOn+xBJXEe051yKKciTx7su+kpdk=
4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
4, 31 -- :content-transfer-encoding;
4, 31 -- b=nTteiF59lr9n+R19R1lnEIld85G87Kx7DP5u4/e+e2ns4/ohCC0R5VpoTrBEflO9mH
4, 31 -- Ypgd3x4liZySEg3awz/RNeCt4asSKHQi8yUTSAJagP71U7cqRAwF3sWjsimvl/H0HVS3
4, 31 -- bC81y9aJovuWHrEJ1DtXrvPUu6icifVtoJCwI=
4, 31 -- MIME-Version: 1.0
4, 31 -- Received: by 10.224.14.205 with SMTP id h13mr1911591qaa.8.1236958129131; Fri,
4, 31 -- 13 Mar 2009 08:28:49 -0700 (PDT)
4, 31 -- Date: Fri, 13 Mar 2009 11:28:49 -0400
4, 31 -- Message-ID: {25e2b0d20903130828x7b3846bep7f7907c5d58515bc-at-mail.gmail.com}
4, 31 -- Subject: Strip Aperture holder for ISI ABT-55
4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
4, 31 -- To: microscopy-at-microscopy.com
4, 31 -- Content-Type: text/plain; charset=ISO-8859-1
4, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Fri, 13 Mar 2009 10:46:36 -0500
Subject: [Microscopy] Inter-American meeting on microscopy, Rosario, Argentina, next October

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

CIASEM 2009

10th Inter-American Congress of Electron Microscopy, 2009
Rosario, Argentina

October 25-28 2009

Every two years, CIASEM - the microscopy organization for the western
hemisphere - holds the principal regional meeting on microscopy. The
next meeting in the series will be held in Rosario, Argentina. The
dates are October 25-28 2009.

This reminder is to let you know that the date for submission of
abstracts is just a week away: March 20, 2009. If this sounds like not
enough time, let me draw to your attention that the abstract required
now is not the normal two-page abstract, but is a 200-word short
abstract. The extended two-page abstract is not due until June.

These are exciting meetings. I would like to encourage you to attend.

Full information is available at the Congress web site:
http://www.ciasem2009.com.ar/

The site for CIASEM in general (which includes, for example, the
proceedings of the previous meeting) is:
http://www.ciasem.com/


Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
11, 21 -- From jae5-at-lehigh.edu Fri Mar 13 10:46:36 2009
11, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DFkZ39017191
11, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 10:46:35 -0500
11, 21 -- Received: from [127.0.0.1] (r054059.mat.Lehigh.EDU [128.180.54.59])
11, 21 -- (authenticated bits=0)
11, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n2DFkXEt007074
11, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
11, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 11:46:34 -0400
11, 21 -- Message-ID: {49BA7FD9.5010000-at-lehigh.edu}
11, 21 -- Date: Fri, 13 Mar 2009 11:46:33 -0400
11, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
11, 21 -- Organization: Lehigh University
11, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
11, 21 -- MIME-Version: 1.0
11, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
11, 21 -- Subject: Inter-American meeting on microscopy, Rosario, Argentina, next October
11, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 21 -- Content-Transfer-Encoding: 7bit
11, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu
11, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Fri, 13 Mar 2009 13:17:24 -0500
Subject: [Microscopy] Fast monochrome digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have our LN2 filling station in a smallish room off our loading dock with
an O2 sensor/alarm. With the doors open and a fan moving the cold N2
vapours out off the floor, O2 levels will drop from 20.8% (by the meter
read-out) to 20.1% when filling a cold 10L dewar

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca


-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk
[mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Friday, March 13, 2009 5:29 AM
To: rjharris-at-uwo.ca

Bill

I'm sorry but I have to take you to task about climbers at and above
6.5 km. First of all THEY MUST ACCLIMATIZE usually by walking there
and secondly a significant number of people have problems with the
reduced levels of oxygen at those altitudes. If you took the best
mountain climbers in the world and dropped them (gently) on Mount
Everest without acclimatizing they would be dead in minutes. Similarly
I think with levels of oxygen below about 16% for most people.

Perhaps if people equated a low oxygen level with the
mountaineer's "Death Zone" it would put things into perspective.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: tivol-at-caltech.edu

I have not seen high resolution b/w or color cameras that
are that fast either. Depending on why one would want a
high frame rate, you might consider:

http://www.pixera.com/products/penguin-clm/penguin%20clm.htm

It is 1.5M pixels, 0.002Lux sensitivity and 30fps at 640x480
but noiseless capture at 1.5M pixels. Last time I sold one,
they were about $4500US. For most work, high pixel count is
not necessary. I have two new Penguin 150CL cameras as residual
units that I don't need. If anyone is interested in these,
they can contact me off-line.

gary g.


At 03:29 AM 3/13/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
11, 22 -- From gary-at-gaugler.com Fri Mar 13 13:17:23 2009
11, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2DIHJsh019267
11, 22 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 13:17:21 -0500
11, 22 -- Message-Id: {200903131817.n2DIHJsh019267-at-ns.microscopy.com}
11, 22 -- Received: (qmail 26321 invoked from network); 13 Mar 2009 11:22:52 -0700
11, 22 -- Received: by simscan 1.1.0 ppid: 26318, pid: 26319, t: 0.1448s
11, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 22 -- by smtp1 with SMTP; 13 Mar 2009 11:22:52 -0700
11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 22 -- Date: Fri, 13 Mar 2009 11:17:01 -0700
11, 22 -- To: kjmorris-at-well.ox.ac.uk
11, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 22 -- Subject: Re: [Microscopy] RE: Fast monochrome digital camera
11, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 22 -- In-Reply-To: {200903131029.n2DATXAE017919-at-ns.microscopy.com}
11, 22 -- References: {200903131029.n2DATXAE017919-at-ns.microscopy.com}
11, 22 -- Mime-Version: 1.0
11, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
11, 22 -- Content-Transfer-Encoding: 8bit
11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DIHJsh019267
==============================End of - Headers==============================




From: astamand-at-phycotech.com
Date: Fri, 13 Mar 2009 13:27:45 -0500
Subject: [Microscopy] SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All, I am looking at purchasing a Phenom SEM by FEI. Does anyone
have experience with this instrument and what do you think? I'd be
using it for confirming diatom identifications. Thanks, Ann.

--
Ann St. Amand, Ph.D., CLP
President
PhycoTech, Inc
620 Broad St., Suite 100
St. Joseph, MI 49085

voice. 269-983-3654
fax. 269-983-3653
email. astamand-at-phycotech.com
web. www.phycotech.com


==============================Original Headers==============================
4, 18 -- From astamand-at-phycotech.com Fri Mar 13 13:27:45 2009
4, 18 -- Received: from smtp2.wmis.net (smtp2.wmis.net [216.109.194.25])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DIRiO4000830
4, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 13:27:44 -0500
4, 18 -- Received: from [127.0.0.1] (unknown [216.109.204.194])
4, 18 -- by smtp2.wmis.net (Postfix) with ESMTP id A39F81296C2
4, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 14:27:43 -0400 (EDT)
4, 18 -- Message-ID: {49BAA59F.10807-at-phycotech.com}
4, 18 -- Date: Fri, 13 Mar 2009 14:27:43 -0400
4, 18 -- From: "Ann St. Amand" {astamand-at-phycotech.com}
4, 18 -- Reply-To: astamand-at-phycotech.com
4, 18 -- Organization: PhycoTech, Inc.
4, 18 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
4, 18 -- MIME-Version: 1.0
4, 18 -- To: Microscopy-at-microscopy.com
4, 18 -- Subject: SEM
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Fri, 13 Mar 2009 15:30:02 -0500
Subject: [Microscopy] nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Larry;
You raise another point about hypoxia: high altitude pilots are
trained to recognize the symptoms. The symptoms are so subtle, most
people don't recognize them and don't know they are about to pass out.
Interestingly, this was first recognized shortly after WW2 by Charles
Lindberg when he was working as a test pilot in the new jet fighters.

John Mardinly,
Numonyx

-----Original Message-----
X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu]
Sent: Thursday, March 12, 2009 3:25 PM
To: MARDINLY, A

Sometimes good intentions go awry as well. Some years ago when a freezer

or dewar failed a lab person stored items in a portable dewar and then
placed it in a cold room. It cold in there and should last
longer--right? Later someone else entered the room and nearly passed out

due to the lack of oxygen. He was smart and had experience at high
altitudes so managed to get out and survive. Whew!
Larry

jkrupp-at-deltacollege.edu wrote:
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
}
} You should also not discount the possibility of a pressure relief
} valve failure or poor insulation causing higher than normal boil off.
}
} I once had a tank's high pressure safety go off. Sounded like a jet
} plane and it filled the room with nitrogen gas in a matter of minutes.

} Called the fire department and they advised us to evacuate the room
} and go outside. The came screaming up with full breathing apparat. and

} checked the room for us before we went back in. No harm done, but
} pretty exciting.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original
Headers==============================
} 8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009
} 8, 44 -- Received: from mailin.deltacollege.edu
(mailin.deltacollege.edu [207.62.178.150])
} 8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n2CLt5iP026014
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
16:55:18 -0500
} 8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain
[127.0.0.1])
} 8, 44 -- by localhost (Email Security Appliance) with SMTP id
CB0691B9464_9B97F17B
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
21:31:03 +0000 (GMT)
} 8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us
[207.62.178.236])
} 8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with
ESMTP id 8C8E3183B0B_9B97F17F
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
21:31:01 +0000 (GMT)
} 8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} 8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu,
12 Mar 2009 14:54:11 -0700
} 8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} 8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server
4.15) with
} 8, 44 -- ESMTP id KGEXEU00.2M0 for
{microscopy-at-microscopy.com} ; Thu, 12
} 8, 44 -- Mar 2009 14:37:42 -0700
} 8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id
0F9BA8F7501D
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
14:54:11 -0700 (PDT)
} 8, 44 -- X-Virus-Scanned: amavisd-new at
} 8, 44 -- X-Spam-Flag: NO
} 8, 44 -- X-Spam-Score: -2.499
} 8, 44 -- X-Spam-Level:
} 8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6
tests=[AWL=0.000,
} 8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1]
} 8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1])
(amavisd-new, port 10024)
} 8, 44 -- with ESMTP id nPmPguQidFlT for
{microscopy-at-microscopy.com} ;
} 8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT)
} 8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146])
} 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id
2AE0A8F7501E
} 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
14:54:10 -0700 (PDT)
} 8, 44 -- Message-Id:
{21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu}
} 8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} 8, 44 -- To: microscopy-at-microscopy.com
} 8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
} 8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
} 8, 44 -- Content-Transfer-Encoding: 7bit
} 8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope
} 8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700
} 8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com}
} 8, 44 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of -
Headers==============================
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original
Headers==============================
6, 41 -- From Larry.Ackerman-at-ucsf.edu Thu Mar 12 17:19:22 2009
6, 41 -- Received: from emfmcb01.ucsfmedicalcenter.org
(EMFMCB01.ucsfmedicalcenter.org [64.54.46.97])
6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2CMJLWJ008140
6, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
17:19:22 -0500
6, 41 -- Received: from [64.54.35.209] by emfmcb01.ucsfmedicalcenter.org
with
6, 41 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall
v6.3.2));
6, 41 -- Thu, 12 Mar 2009 15:19:17 -0700
6, 41 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
6, 41 -- X-AuditID: 403623d1-a7451bb00000708a-90-49b98a655eb0
6, 41 -- Received: from EXHT02.net.ucsf.edu (unknown [64.54.247.219]) by
6, 41 -- vsobmcb01.ucsfmedicalcenter.org (Symantec Mail Security) with
ESMTP id
6, 41 -- 27FF715C7 for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009
15:19:17
6, 41 -- -0700 (PDT)
6, 41 -- Received: from EXVS05.net.ucsf.edu (64.54.128.150) by
6, 41 -- EXHT02.net.ucsf.edu (64.54.247.219) with Microsoft SMTP Server
id
6, 41 -- 8.1.340.0; Thu, 12 Mar 2009 15:19:16 -0700
6, 41 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
6, 41 -- EXVS05.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959);
Thu, 12 Mar
6, 41 -- 2009 15:18:29 -0700
6, 41 -- Received: from Ralston-Lab-Larry-Ackerman.local
([128.218.123.88]) by
6, 41 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959);
Thu, 12 Mar
6, 41 -- 2009 15:18:28 -0700
6, 41 -- Message-ID: {49B98A34.2010006-at-ucsf.edu}
6, 41 -- Date: Thu, 12 Mar 2009 15:18:28 -0700
6, 41 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
6, 41 -- Reply-to: larry.ackerman-at-ucsf.edu
6, 41 -- Organization: UCSF, NeuroAnatomy
6, 41 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 41 -- MIME-Version: 1.0
6, 41 -- To: Microscopy-at-microscopy.com
6, 41 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope
6, 41 -- References: {200903122203.n2CM3spi003857-at-ns.microscopy.com}
6, 41 -- In-Reply-To: {200903122203.n2CM3spi003857-at-ns.microscopy.com}
6, 41 -- X-OriginalArrivalTime: 12 Mar 2009 22:18:28.0719 (UTC)
6, 41 -- FILETIME=[784BC7F0:01C9A360]
6, 41 -- X-Brightmail-Tracker: AAAAAQ3stXE=
6, 41 -- X-WSS-ID: 65A755EF1O868741-01-01
6, 41 -- Content-Type: text/plain;
6, 41 -- charset=iso-8859-1;
6, 41 -- format=flowed
6, 41 -- Content-Transfer-Encoding: 7bit
==============================End of -
Headers==============================



==============================Original Headers==============================
19, 27 -- From A.MARDINLY-at-numonyx.com Fri Mar 13 15:30:02 2009
19, 27 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DKU2Mb019096
19, 27 -- for {Microscopy-at-Microscopy.com} ; Fri, 13 Mar 2009 15:30:02 -0500
19, 27 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24])
19, 27 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 6BBFA144004
19, 27 -- for {Microscopy-at-Microscopy.com} ; Fri, 13 Mar 2009 14:32:35 -0400 (EDT)
19, 27 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
19, 27 -- Fri, 13 Mar 2009 16:30:01 -0400
19, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 27 -- Content-class: urn:content-classes:message
19, 27 -- MIME-Version: 1.0
19, 27 -- Content-Type: text/plain;
19, 27 -- charset="us-ascii"
19, 27 -- Subject: RE: [Microscopy] nitrogen supply at the microscope
19, 27 -- Date: Fri, 13 Mar 2009 16:30:00 -0400
19, 27 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39D87-at-EXDRESBENMX012.numonyx.local}
19, 27 -- X-MS-Has-Attach:
19, 27 -- X-MS-TNEF-Correlator:
19, 27 -- Thread-Topic: [Microscopy] nitrogen supply at the microscope
19, 27 -- Thread-Index: AcmjYVt1LO2mWcXsSJWOUp2KYteyawAuFvywAAAuocA=
19, 27 -- References: {200903122224.n2CMOhut019527-at-ns.microscopy.com}
19, 27 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
19, 27 -- To: {Microscopy-at-Microscopy.com}
19, 27 -- X-OriginalArrivalTime: 13 Mar 2009 20:30:01.0830 (UTC) FILETIME=[7C4D1060:01C9A41A]
19, 27 -- Content-Transfer-Encoding: 8bit
19, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DKU2Mb019096
==============================End of - Headers==============================




From: Keri.Colwell-at-inspection.gc.ca
Date: Fri, 13 Mar 2009 15:33:07 -0500
Subject: [Microscopy] Images of fast red

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I'm trying to locate some images of crystal fall out when staining with fast red. Does anyone know where I can look?



Keri Colwell
Laboratory Technologist
CFIA - Lethbridge Laboratory
P.O. Box 640
Lethbridge, AB
T1J 3Z4
Phone/ Téléphone (403) 382-5500 ext. 5613/ Facsimile/Telecopier: (403) 381-1202
Email - keri.colwell-at-inspection.gc.ca
Government of Canada/ Gouvernment du Canada
www.inspection.gc.ca



_______________________________________________
Histonet mailing list
Histonet-at-lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



==============================Original Headers==============================
10, 15 -- From Keri.Colwell-at-inspection.gc.ca Fri Mar 13 15:33:07 2009
10, 15 -- Received: from agrpazsmtp8.agr.gc.ca (agrpazsmtp8.agr.gc.ca [192.197.71.119])
10, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DKX6Aq022798
10, 15 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Mar 2009 15:33:07 -0500
10, 15 -- Message-Id: {s9ba8ac1.052-at-inspection.gc.ca}
10, 15 -- X-Mailer: Novell GroupWise Internet Agent 6.5.7
10, 15 -- Date: Fri, 13 Mar 2009 16:32:57 -0400
10, 15 -- From: "Keri Colwell" {Keri.Colwell-at-inspection.gc.ca}
10, 15 -- To: {Microscopy-at-Microscopy.Com}
10, 15 -- Subject: Images of fast red
10, 15 -- Mime-Version: 1.0
10, 15 -- Content-Type: text/plain; charset=UTF-8
10, 15 -- Content-Disposition: inline
10, 15 -- Content-Transfer-Encoding: 8bit
10, 15 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DKX6Aq022798
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Fri, 13 Mar 2009 16:17:08 -0500
Subject: [Microscopy] RE: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have one and we're very happy with it. The ease of use is
outstanding, and the depth-of-field we can achieve is extremely useful.
FEI's customer service is world-class, as well. We have used it to look
at diatoms with good results (we use them for filters).

I am finding, however, that once you have a SEM, you start seeing a
"need" for many other pieces of equipment that you might never have
acquired otherwise (sputter coaters, microtomes, diamond saws, etc.) It
is also not a particularly quiet instrument. Although I have minimal
experience with other EM's to compare it to, it's much noisier than my
light microscope.

I have no connection to FEI other than as a satisfied customer.

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.

-----Original Message-----
X-from: astamand-at-phycotech.com [mailto:astamand-at-phycotech.com]
Sent: Friday, March 13, 2009 1:32 PM
To: Zonis, Robert

Poor Charles Lindbergh; he is credited with so much that he never did....
The first high altitude studies of hypoxia (not counting anecdotal reports
from early mountain climbers-- remember poor Ötzi) were conducted by the
British Association for the Advancement of Science in the 1850's (yes, 19th
century) using balloons. The first serious encounter with hypoxia by a
balloonist occurred in 1804, by one Joseph Louis Gay Lussac, who made
several flights above 20,000 feet/6000 meters. I am hardly an expert in
aviation (or anything else for that matter) but in the age of Google, I have
a compulsion for checking facts before going public....

Cheers
Roger

} From: A.MARDINLY-at-numonyx.com
} Reply-To: A.MARDINLY-at-numonyx.com
} Date: Fri, 13 Mar 2009 15:38:20 -0500
} To: raristau-at-ims.uconn.edu
} Subject: [Microscopy] RE: nitrogen supply at the microscope
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Larry;
} You raise another point about hypoxia: high altitude pilots are
} trained to recognize the symptoms. The symptoms are so subtle, most
} people don't recognize them and don't know they are about to pass out.
} Interestingly, this was first recognized shortly after WW2 by Charles
} Lindberg when he was working as a test pilot in the new jet fighters.
}
} John Mardinly,
} Numonyx
}



==============================Original Headers==============================
5, 18 -- From raristau-at-ims.uconn.edu Fri Mar 13 16:17:08 2009
5, 18 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DLH7RZ004625
5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 16:17:07 -0500
5, 18 -- Received: from [137.99.19.228] (d19h228.public.uconn.edu [137.99.19.228])
5, 18 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2DLH7YJ025106
5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 17:17:07 -0400
5, 18 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
5, 18 -- Date: Fri, 13 Mar 2009 17:14:30 -0400
5, 18 -- Subject: RE: nitrogen supply at the microscope
5, 18 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
5, 18 -- To: {microscopy-at-microscopy.com}
5, 18 -- Message-ID: {C5E044F6.48B1%raristau-at-ims.uconn.edu}
5, 18 -- In-Reply-To: {200903132038.n2DKcK9B007717-at-ns.microscopy.com}
5, 18 -- Mime-version: 1.0
5, 18 -- Content-type: text/plain; charset="ISO-8859-1"
5, 18 -- Content-Transfer-Encoding: 8bit
5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DLH7RZ004625
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Sun, 15 Mar 2009 04:33:30 -0500
Subject: [Microscopy] RE: nitrogen supply at the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger;
Maybe I should have clarified the hypoxia description as rapid onset-something mountain climbers and ballooners never experienced. My reference is the book "Lindbergh" by A. Scott Berg, Putnams, NY, 1998. Starting on Page 446, there is a description of work Lindbergh did with Dr. Walter M. Boothby at the Mayo Clinic in 1942. Both were concerned with rapid changes in pressure and oxygen at the 40,000 foot level, situations never encountered by man until the onset of high performance aircraft. Initial testing was done over a 10 day period in a ground based chamber capable of rapid evacuation. During that time. Lindbergh challenged the prevailing opinion that no one could be trained to recognize the rapid onset of hypoxia to such an extent that they could do anything about it. Subsequent testing was done in P-47 aircraft. One situation occurred in which his oxygen tank ran out at the same time an altimeter malfunctioned and he recognized the symptoms and put the plane into a steep dive as he lost consciousness gave credence to his theory. So Roger, I love Google too, just read a book every once and a while. For microscopists, the lesson is that they never undergo training to recognize hypoxia, and a sudden release of nitrogen or just walking into a room with the oxygen level below what will sustain consciousness is extremely dangerous. They will just conk out, rather than run for the exit.


John Mardinly,
Numonyx


-----Original Message-----
X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu]
Sent: Friday, March 13, 2009 2:23 PM
To: MARDINLY, A

Poor Charles Lindbergh; he is credited with so much that he never did....
The first high altitude studies of hypoxia (not counting anecdotal reports
from early mountain climbers-- remember poor Ötzi) were conducted by the
British Association for the Advancement of Science in the 1850's (yes, 19th
century) using balloons. The first serious encounter with hypoxia by a
balloonist occurred in 1804, by one Joseph Louis Gay Lussac, who made
several flights above 20,000 feet/6000 meters. I am hardly an expert in
aviation (or anything else for that matter) but in the age of Google, I have
a compulsion for checking facts before going public....

Cheers
Roger

} From: A.MARDINLY-at-numonyx.com
} Reply-To: A.MARDINLY-at-numonyx.com
} Date: Fri, 13 Mar 2009 15:38:20 -0500
} To: raristau-at-ims.uconn.edu
} Subject: [Microscopy] RE: nitrogen supply at the microscope
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Larry;
} You raise another point about hypoxia: high altitude pilots are
} trained to recognize the symptoms. The symptoms are so subtle, most
} people don't recognize them and don't know they are about to pass out.
} Interestingly, this was first recognized shortly after WW2 by Charles
} Lindberg when he was working as a test pilot in the new jet fighters.
}
} John Mardinly,
} Numonyx
}



==============================Original Headers==============================
5, 18 -- From raristau-at-ims.uconn.edu Fri Mar 13 16:17:08 2009
5, 18 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DLH7RZ004625
5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 16:17:07 -0500
5, 18 -- Received: from [137.99.19.228] (d19h228.public.uconn.edu [137.99.19.228])
5, 18 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2DLH7YJ025106
5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 17:17:07 -0400
5, 18 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
5, 18 -- Date: Fri, 13 Mar 2009 17:14:30 -0400
5, 18 -- Subject: RE: nitrogen supply at the microscope
5, 18 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
5, 18 -- To: {microscopy-at-microscopy.com}
5, 18 -- Message-ID: {C5E044F6.48B1%raristau-at-ims.uconn.edu}
5, 18 -- In-Reply-To: {200903132038.n2DKcK9B007717-at-ns.microscopy.com}
5, 18 -- Mime-version: 1.0
5, 18 -- Content-type: text/plain; charset="ISO-8859-1"
5, 18 -- Content-Transfer-Encoding: 8bit
5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DLH7RZ004625
==============================End of - Headers==============================



==============================Original Headers==============================
15, 29 -- From A.MARDINLY-at-numonyx.com Sun Mar 15 04:33:30 2009
15, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38])
15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2F9XUZd024538
15, 29 -- for {Microscopy-at-Microscopy.com} ; Sun, 15 Mar 2009 04:33:30 -0500
15, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22])
15, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 9CBCF4143C0;
15, 29 -- Sun, 15 Mar 2009 04:51:02 -0400 (EDT)
15, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
15, 29 -- Sun, 15 Mar 2009 05:33:29 -0400
15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
15, 29 -- Content-class: urn:content-classes:message
15, 29 -- MIME-Version: 1.0
15, 29 -- Content-Type: text/plain;
15, 29 -- charset="iso-8859-1"
15, 29 -- Subject: RE: [Microscopy] RE: nitrogen supply at the microscope
15, 29 -- Date: Sun, 15 Mar 2009 05:33:27 -0400
15, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39E27-at-EXDRESBENMX012.numonyx.local}
15, 29 -- In-Reply-To: {200903132123.n2DLNNVq017094-at-ns.microscopy.com}
15, 29 -- X-MS-Has-Attach:
15, 29 -- X-MS-TNEF-Correlator:
15, 29 -- Thread-Topic: [Microscopy] RE: nitrogen supply at the microscope
15, 29 -- Thread-Index: AcmkIfS2NgDLQbZLSpKo+n/noBg2QQBKvWGw
15, 29 -- References: {200903132123.n2DLNNVq017094-at-ns.microscopy.com}
15, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
15, 29 -- To: {raristau-at-ims.uconn.edu}
15, 29 -- Cc: {Microscopy-at-Microscopy.com}
15, 29 -- X-OriginalArrivalTime: 15 Mar 2009 09:33:29.0546 (UTC) FILETIME=[197F0AA0:01C9A551]
15, 29 -- Content-Transfer-Encoding: 8bit
15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2F9XUZd024538
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Sun, 15 Mar 2009 12:12:55 -0500
Subject: [Microscopy] RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There have been a lot of comments on the dangers of hypoxia from
leaking or defective liquid nitrogen tanks. All very interesting.

QUESTION: Are there any straightforward symptoms of the onset of
hypoxia that can be recognized in time to avoid succumbing to the
situation??
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
2, 14 -- From bigelow-at-umich.edu Sun Mar 15 12:12:54 2009
2, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82])
2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHCqTn025128
2, 14 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:12:53 -0500
2, 14 -- Received: FROM [76.234.131.113] (adsl-76-234-131-113.dsl.sfldmi.sbcglobal.net [76.234.131.113])
2, 14 -- BY hellskitchen.mr.itd.umich.edu ID 49BD3711.9A774.25996 ;
2, 14 -- 15 Mar 2009 13:12:50 -0400
2, 14 -- Mime-Version: 1.0
2, 14 -- Message-Id: {p06240800c5e2e6aed43a-at-[99.130.26.88]}
2, 14 -- Date: Sun, 15 Mar 2009 13:12:46 -0400
2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
2, 14 -- From: Wil Bigelow {bigelow-at-umich.edu}
2, 14 -- Subject: [Microscopy] RE: symptoms of hypoxia
2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lamiller-at-illinois.edu
Date: Sun, 15 Mar 2009 12:41:44 -0500
Subject: [Microscopy] Re: RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm not certain of the official guidelines for symptoms, but they may be
similar to asthma, as it's also a condition of low O2 when flared.


__ blacking out, or vision seeming to de-pixil some.

__ Closing one's eyes after seeing a contrasted object, and still
retaining the image for longer than normal when the eyes are closed.

__Slurred speech & slowed thinking

__ sometimes some dyslexia ( when I start mixing words it's time for the
inhaler)

__ sometimes even stumbling on your own feet.

__fatigue, that goes away when you are not near the trigger

__ Nausea, vertigo



Lou Ann



bigelow-at-umich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} There have been a lot of comments on the dangers of hypoxia from
} leaking or defective liquid nitrogen tanks. All very interesting.
}
} QUESTION: Are there any straightforward symptoms of the onset of
} hypoxia that can be recognized in time to avoid succumbing to the
} situation??
}


==============================Original Headers==============================
16, 21 -- From lamiller-at-illinois.edu Sun Mar 15 12:41:43 2009
16, 21 -- Received: from QMTA04.emeryville.ca.mail.comcast.net (qmta04.emeryville.ca.mail.comcast.net [76.96.30.40])
16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHfgCP007104
16, 21 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:41:42 -0500
16, 21 -- Received: from OMTA04.emeryville.ca.mail.comcast.net ([76.96.30.35])
16, 21 -- by QMTA04.emeryville.ca.mail.comcast.net with comcast
16, 21 -- id TTmD1b0070lTkoCA4Vhjvq; Sun, 15 Mar 2009 17:41:43 +0000
16, 21 -- Received: from lou-anns-computer.local ([98.212.148.206])
16, 21 -- by OMTA04.emeryville.ca.mail.comcast.net with comcast
16, 21 -- id TVhh1b0044TRewa8QVhhUW; Sun, 15 Mar 2009 17:41:42 +0000
16, 21 -- Message-ID: {49BD3DD3.9050402-at-illinois.edu}
16, 21 -- Date: Sun, 15 Mar 2009 12:41:39 -0500
16, 21 -- From: Lou Ann Miller {lamiller-at-illinois.edu}
16, 21 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
16, 21 -- MIME-Version: 1.0
16, 21 -- To: bigelow-at-umich.edu, microscopy-at-microscopy.com
16, 21 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia
16, 21 -- References: {200903151729.n2FHTKUa004625-at-ns.microscopy.com}
16, 21 -- In-Reply-To: {200903151729.n2FHTKUa004625-at-ns.microscopy.com}
16, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
16, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jquinn-at-www.matscieng.sunysb.edu
Date: Sun, 15 Mar 2009 13:59:21 -0500
Subject: [Microscopy] re: LN2 in confined space

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks -

Personally, I would like this thread to
turn away from "hypoxia", the history,
or symptoms.

The MSDS, fire code, etc.... clearly state
"do not store LN2 is confined spaces". Additionally,
there must be excellent ventilation.

Some suggest to wear a self-contained breathing
apparatus if the oxygen content routinely falls
below 19%.

In New York City, you cannot legally keep a dewar
within a few feet of a door or exit. Infact, it
is illegal there to store LN2 is a public hallway.

Hence, let us be preventive. An oxygen monitor from
MSA (Mine Safety Appliances) can be bought from
Lab Safety Supply, Fisher Sci, etc... for just $200.

regards,

JQuinn



==============================Original Headers==============================
11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009
11, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33])
11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FIxLPu022978
11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21 -0500
11, 12 -- Received: (from jquinn-at-localhost)
11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350
11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53 -0500
11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500
11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu}
11, 12 -- To: microscopy-at-microscopy.com
11, 12 -- Subject: re: LN2 in confined space
==============================End of - Headers==============================




From: michael-at-shaffer.net
Date: Mon, 16 Mar 2009 04:55:02 -0500
Subject: [Microscopy] RE: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ann writes ...

} Hi All, I am looking at purchasing a Phenom SEM by FEI. Does
} anyone have experience with this instrument and what do you
} think? I'd be using it for confirming diatom
} identifications. Thanks, Ann.

It seems to me this countertop SEM images with backscattered electrons only,
which may be a bit contrasty for imaging extremely 3D subjects (eg,
diatoms). Have FEI image a couple of your samples for you.

hth, Michael Shaffer :o)

SEM/MLA Research Coordinator
http://www.mun.ca/creait/maf/
Inco Innovation Centre
Memorial University
St. John's, Newfoundland



__________ Information from ESET NOD32 Antivirus, version of virus signature
database 3937 (20090314) __________

The message was checked by ESET NOD32 Antivirus.

http://www.eset.com



==============================Original Headers==============================
10, 29 -- From michael-at-shaffer.net Mon Mar 16 04:55:02 2009
10, 29 -- Received: from smtprelay.hostedemail.com (smtprelay0139.hostedemail.com [216.40.44.139])
10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2G9t29q016055
10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 04:55:02 -0500
10, 29 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254])
10, 29 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id A57EF1DDDA04;
10, 29 -- Mon, 16 Mar 2009 09:55:01 +0000 (UTC)
10, 29 -- X-SpamScore: 1
10, 29 -- X-Spam-Summary: 50,0,0,0839f5eead2c7a27,0d355e4b7ffc0630,michael-at-shaffer.net,astamand-at-phycotech.com:microscopy-at-microscopy.com,RULES_HIT:10:355:379:539:541:542:599:601:945:967:973:988:989:1155:1160:1260:1277:1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1540:1593:1594:1711:1730:1747:1766:1792:2234:2378:2393:2525:2553:2560:2564:2682:2685:2857:2859:2892:2933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3770:3865:3866:3867:3868:3869:3872:3873:3876:3877:3934:3936:3938:3941:3944:3947:3950:3953:3956:3959:4250:4321:4659:4699:4860:5007:6114:6261:7679:7903:8501:8985:9025:9388,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none
10, 29 -- Received: from rarewolf (CPE001d7e3e1515-CM0018682b4b4c.cpe.net.cable.rogers.com [72.139.95.151])
10, 29 -- (Authenticated sender: michael-at-shaffer.net)
10, 29 -- by omf06.hostedemail.com (Postfix) with ESMTP;
10, 29 -- Mon, 16 Mar 2009 09:55:01 +0000 (UTC)
10, 29 -- From: "michael shaffer" {michael-at-shaffer.net}
10, 29 -- To: {astamand-at-phycotech.com}
10, 29 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com}
10, 29 -- References: {200903131828.n2DIS7OM001444-at-ns.microscopy.com}
10, 29 -- Subject: RE: [Microscopy] SEM
10, 29 -- Date: Mon, 16 Mar 2009 07:24:58 -0230
10, 29 -- Message-ID: {5EF33F2F45F24A34B82694993BCCC84F-at-rarewolf}
10, 29 -- MIME-Version: 1.0
10, 29 -- Content-Type: text/plain;
10, 29 -- charset="US-ASCII"
10, 29 -- Content-Transfer-Encoding: 7bit
10, 29 -- X-Mailer: Microsoft Office Outlook 11
10, 29 -- In-Reply-To: {200903131828.n2DIS7OM001444-at-ns.microscopy.com}
10, 29 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
10, 29 -- Thread-Index: AcmkCXTru/iPA2+uRMWLGmDDqSCXYwCE1CdA
10, 29 -- X-session-marker: 6D69636861656C40736861666665722E6E6574
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Mon, 16 Mar 2009 08:04:08 -0500
Subject: [Microscopy] Free TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A friend has asked me to post this notice. Please contact him
directly

--

Hi-

I need to ask if you could post an ad to the EM listserve.



We have a Hitachi H-600 TEM that is free for the taking.

The scope is in good condition and is located in Macon GA

If interested, please contact :

Mike Horst (478)301-2558



Thanks-

M



Michael N. Horst, Ph.D.

Mercer University School of Medicine

1550 College Street

Macon GA 31207 USA

(


==============================Original Headers==============================
22, 22 -- From gwe-at-ufl.edu Mon Mar 16 08:04:08 2009
22, 22 -- Received: from smtp.ufl.edu (smtp04.osg.ufl.edu [128.227.74.71])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GD48iA004005
22, 22 -- for {microscopy-at-microscopy.org} ; Mon, 16 Mar 2009 08:04:08 -0500
22, 22 -- Received: from osgjas02.cns.ufl.edu (osgjas02.cns.ufl.edu [128.227.74.132])
22, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n2GD47H9000439
22, 22 -- for {microscopy-at-microscopy.org} ; Mon, 16 Mar 2009 09:04:07 -0400
22, 22 -- Message-ID: {1898119412.73221237208647649.JavaMail.osg-at-osgjas02.cns.ufl.edu}
22, 22 -- Date: Mon, 16 Mar 2009 09:04:07 -0400 (EDT)
22, 22 -- From: greg erdos {gwe-at-ufl.edu}
22, 22 -- Reply-To: gwe-at-ufl.edu
22, 22 -- To: microscopy-at-microscopy.org
22, 22 -- Subject: Free TEM
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
22, 22 -- X-Originating-IP: 98.70.37.98 [98.70.37.98]
22, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-03-16_04:2009-03-13,2009-03-16,2009-03-16 signatures=0
22, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=1 spamscore=1 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903160069
22, 22 -- X-Spam-Level: *
22, 22 -- X-UFL-Spam-Level: *
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Mon, 16 Mar 2009 08:14:09 -0500
Subject: [Microscopy] Meeting Announcement: Midwest Microscopy and Microanalysis Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All,

The Midwest Microscopy and Microanalysis Society will hold its first
meeting of 2009 on Friday, March 27th, at Northwestern University in
Evanston, IL. Our theme is "Greater than the Sum: Advances in
Correlative Microscopy". Program details and regstration information
can be found on our website under Meetings:

www.midwestmicroscopy.org

Please join us to hear an excellent group of speakers and to show
support for your Local Affiliate Society as we kick off another year of
activities!


Elaine Schumacher
M3S Program Coodinator


*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com}
Web Site: www.mccrone.com {http://www.mccrone.com/}






==============================Original Headers==============================
12, 23 -- From eschumacher-at-mccrone.com Mon Mar 16 08:14:08 2009
12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GDE5bo017372
12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 08:14:07 -0500
12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959);
12, 23 -- Mon, 16 Mar 2009 08:14:05 -0500
12, 23 -- Content-class: urn:content-classes:message
12, 23 -- MIME-Version: 1.0
12, 23 -- Content-Type: text/plain;
12, 23 -- charset="us-ascii"
12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society
12, 23 -- Date: Mon, 16 Mar 2009 08:13:18 -0500
12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A702089952-at-MCCRONEMSG.tmg.mccrone.com}
12, 23 -- X-MS-Has-Attach:
12, 23 -- X-MS-TNEF-Correlator:
12, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society
12, 23 -- Thread-Index: AcmmOPjtmFFHfYRJSlKcMJ0caaMIgQ==
12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
12, 23 -- To: {microscopy-at-microscopy.com}
12, 23 -- X-OriginalArrivalTime: 16 Mar 2009 13:14:05.0664 (UTC) FILETIME=[15401E00:01C9A639]
12, 23 -- Content-Transfer-Encoding: 8bit
12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GDE5bo017372
==============================End of - Headers==============================




From: jfish-at-gladstone.ucsf.edu
Date: Mon, 16 Mar 2009 11:13:51 -0500
Subject: [Microscopy] Re: RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds like my insulin reactions, or just before I get a migraine!
Jo Dee


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish-at-gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
X-from: lamiller-at-illinois.edu [mailto:lamiller-at-illinois.edu]
Sent: Sunday, March 15, 2009 10:48 AM
To: jfish-at-gladstone.ucsf.edu

I'm not certain of the official guidelines for symptoms, but they may be
similar to asthma, as it's also a condition of low O2 when flared.


__ blacking out, or vision seeming to de-pixil some.

__ Closing one's eyes after seeing a contrasted object, and still
retaining the image for longer than normal when the eyes are closed.

__Slurred speech & slowed thinking

__ sometimes some dyslexia ( when I start mixing words it's time for the
inhaler)

__ sometimes even stumbling on your own feet.

__fatigue, that goes away when you are not near the trigger

__ Nausea, vertigo



Lou Ann



bigelow-at-umich.edu wrote:
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} There have been a lot of comments on the dangers of hypoxia from
} leaking or defective liquid nitrogen tanks. All very interesting.
}
} QUESTION: Are there any straightforward symptoms of the onset of
} hypoxia that can be recognized in time to avoid succumbing to the
} situation??
}


==============================Original Headers==============================
16, 21 -- From lamiller-at-illinois.edu Sun Mar 15 12:41:43 2009
16, 21 -- Received: from QMTA04.emeryville.ca.mail.comcast.net
(qmta04.emeryville.ca.mail.comcast.net [76.96.30.40])
16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2FHfgCP007104
16, 21 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:41:42
-0500
16, 21 -- Received: from OMTA04.emeryville.ca.mail.comcast.net
([76.96.30.35])
16, 21 -- by QMTA04.emeryville.ca.mail.comcast.net with comcast
16, 21 -- id TTmD1b0070lTkoCA4Vhjvq; Sun, 15 Mar 2009 17:41:43 +0000
16, 21 -- Received: from lou-anns-computer.local ([98.212.148.206])
16, 21 -- by OMTA04.emeryville.ca.mail.comcast.net with comcast
16, 21 -- id TVhh1b0044TRewa8QVhhUW; Sun, 15 Mar 2009 17:41:42 +0000
16, 21 -- Message-ID: {49BD3DD3.9050402-at-illinois.edu}
16, 21 -- Date: Sun, 15 Mar 2009 12:41:39 -0500
16, 21 -- From: Lou Ann Miller {lamiller-at-illinois.edu}
16, 21 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209)
16, 21 -- MIME-Version: 1.0
16, 21 -- To: bigelow-at-umich.edu, microscopy-at-microscopy.com
16, 21 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia
16, 21 -- References: {200903151729.n2FHTKUa004625-at-ns.microscopy.com}
16, 21 -- In-Reply-To: {200903151729.n2FHTKUa004625-at-ns.microscopy.com}
16, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
16, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================


==============================Original Headers==============================
25, 27 -- From jfish-at-gladstone.ucsf.edu Mon Mar 16 11:13:50 2009
25, 27 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31])
25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GGDmxB007010
25, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 11:13:50 -0500
25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,373,1233561600";
25, 27 -- d="scan'208";a="1770163"
25, 27 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25])
25, 27 -- by gmail2.ucsf.edu with ESMTP; 16 Mar 2009 09:13:46 -0700
25, 27 -- Received: from [169.230.76.4] (HELO JFISH)
25, 27 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10)
25, 27 -- with ESMTP id 566887528; Mon, 16 Mar 2009 09:13:46 -0700
25, 27 -- Reply-To: {jfish-at-gladstone.ucsf.edu}
25, 27 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu}
25, 27 -- To: {lamiller-at-illinois.edu} , {microscopy-at-microscopy.com}
25, 27 -- References: {200903151748.n2FHm0rX016075-at-ns.microscopy.com}
25, 27 -- Subject: RE: [Microscopy] Re: RE: symptoms of hypoxia
25, 27 -- Date: Mon, 16 Mar 2009 09:13:39 -0700
25, 27 -- Organization: J. David Gladstone Institutes
25, 27 -- Message-ID: {3E8F1A1B1054440BAE475CFF14918C6B-at-JFISH}
25, 27 -- MIME-Version: 1.0
25, 27 -- Content-Type: text/plain;
25, 27 -- charset="US-ASCII"
25, 27 -- Content-Transfer-Encoding: 7bit
25, 27 -- X-Mailer: Microsoft Office Outlook 11
25, 27 -- Thread-Index: AcmlljIKCsv6Hb5cRoKxD2/rudNAewAu9iQg
25, 27 -- In-Reply-To: {200903151748.n2FHm0rX016075-at-ns.microscopy.com}
25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: paul.gerroir-at-xrcc.xeroxlabs.com
Date: Mon, 16 Mar 2009 11:16:00 -0500
Subject: [Microscopy] Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,
I have been wondering if anyone has done a thorough comparison between a
digital microscope, (Hirox, Keyence) and a conventional light microscope
Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is
one type more suited for given application(s)? What about cost?
Portability?

There wasn't much information in the archives.

Paul J. Gerroir

Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xerox.com




==============================Original Headers==============================
8, 32 -- From paul.gerroir-at-xrcc.xeroxlabs.com Mon Mar 16 11:15:59 2009
8, 32 -- Received: from wbmler4.mail.xerox.com (wbmler4.mail.xerox.com [13.13.138.219])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GGFvi9008192
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 11:15:58 -0500
8, 32 -- Received: from wbmlir1.mail.xerox.com (wbmlir1.mail.xerox.com [13.131.8.221])
8, 32 -- by wbmler4.mail.xerox.com (8.14.2/8.13.8) with ESMTP id n2GGFtsn016449
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:55 -0400
8, 32 -- Received: from wbmlir1.mail.xerox.com (localhost [127.0.0.1])
8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id n2GGFbFa009789
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:37 -0400
8, 32 -- Received: from xrcc-sv8.xrcc.xeroxlabs.com ([13.2.46.182])
8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id n2GGFbPn009778
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:37 -0400
8, 32 -- X-XeroxINT-Source-Ip: 13.2.46.182
8, 32 -- X-XeroxINT-Source-Name: [13.2.46.182]
8, 32 -- X-XeroxINT-Reported-Name: xrcc-sv8.xrcc.xeroxlabs.com
8, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 32 -- Content-class: urn:content-classes:message
8, 32 -- MIME-Version: 1.0
8, 32 -- Content-Type: text/plain;
8, 32 -- charset="us-ascii"
8, 32 -- Subject: Light Microscopy
8, 32 -- Date: Mon, 16 Mar 2009 12:15:34 -0400
8, 32 -- Message-ID: {B4FD2284716D6C4DB94239C248E7CE00984D04-at-xrcc-sv8.xrcc.xeroxlabs.com}
8, 32 -- X-MS-Has-Attach:
8, 32 -- X-MS-TNEF-Correlator:
8, 32 -- Thread-Topic: Light Microscopy
8, 32 -- Thread-Index: AcmmUm8HVZNQtYx1QtCN0B8khQD+MA==
8, 32 -- From: "Gerroir, Paul" {paul.gerroir-at-xrcc.xeroxlabs.com}
8, 32 -- To: {Microscopy-at-Microscopy.com}
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GGFvi9008192
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Mon, 16 Mar 2009 11:56:13 -0500
Subject: [Microscopy] Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,

Informally, we've had demonstrations here of several types of
microscopes, including conventional microscopes (Nikon) and digital
microscopes (Keyence).

The three biggest differences we found were 1) Ease of capturing crisp,
focused images at high to very high resolutions 2) the ability of the
digital microscopes to generate images with substantial depth of field,
due to on-the-fly processing of image stacks, and 3) the ability of the
digital microscope to take an image at several angles, so that the
software can produce a 3-d profile/image of the object. If you don't
need the 3-d profiles, and can wait for image stacks to process, a
conventional light microscope with a decent camera, software and
motorized stage/focus would be a less expensive (but somewhat fuzzier)
alternative.

Compared to the Keyence system, you are also giving up some portability;
I don't know how much that would mean to you.

I have no connection to any of these companies.

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.


-----Original Message-----
X-from: paul.gerroir-at-xrcc.xeroxlabs.com
[mailto:paul.gerroir-at-xrcc.xeroxlabs.com]
Sent: Monday, March 16, 2009 11:26 AM
To: Zonis, Robert

All,
I have been wondering if anyone has done a thorough comparison between a
digital microscope, (Hirox, Keyence) and a conventional light microscope
Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is
one type more suited for given application(s)? What about cost?
Portability?

There wasn't much information in the archives.

Paul J. Gerroir

Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xerox.com




==============================Original
Headers==============================
8, 32 -- From paul.gerroir-at-xrcc.xeroxlabs.com Mon Mar 16 11:15:59 2009
8, 32 -- Received: from wbmler4.mail.xerox.com (wbmler4.mail.xerox.com
[13.13.138.219])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2GGFvi9008192
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009
11:15:58 -0500
8, 32 -- Received: from wbmlir1.mail.xerox.com (wbmlir1.mail.xerox.com
[13.131.8.221])
8, 32 -- by wbmler4.mail.xerox.com (8.14.2/8.13.8) with ESMTP id
n2GGFtsn016449
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009
12:15:55 -0400
8, 32 -- Received: from wbmlir1.mail.xerox.com (localhost [127.0.0.1])
8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id
n2GGFbFa009789
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009
12:15:37 -0400
8, 32 -- Received: from xrcc-sv8.xrcc.xeroxlabs.com ([13.2.46.182])
8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id
n2GGFbPn009778
8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009
12:15:37 -0400
8, 32 -- X-XeroxINT-Source-Ip: 13.2.46.182
8, 32 -- X-XeroxINT-Source-Name: [13.2.46.182]
8, 32 -- X-XeroxINT-Reported-Name: xrcc-sv8.xrcc.xeroxlabs.com
8, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 32 -- Content-class: urn:content-classes:message
8, 32 -- MIME-Version: 1.0
8, 32 -- Content-Type: text/plain;
8, 32 -- charset="us-ascii"
8, 32 -- Subject: Light Microscopy
8, 32 -- Date: Mon, 16 Mar 2009 12:15:34 -0400
8, 32 -- Message-ID:
{B4FD2284716D6C4DB94239C248E7CE00984D04-at-xrcc-sv8.xrcc.xeroxlabs.com}
8, 32 -- X-MS-Has-Attach:
8, 32 -- X-MS-TNEF-Correlator:
8, 32 -- Thread-Topic: Light Microscopy
8, 32 -- Thread-Index: AcmmUm8HVZNQtYx1QtCN0B8khQD+MA==
8, 32 -- From: "Gerroir, Paul" {paul.gerroir-at-xrcc.xeroxlabs.com}
8, 32 -- To: {Microscopy-at-Microscopy.com}
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2GGFvi9008192
==============================End of -
Headers==============================

This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
24, 34 -- From Robert.Zonis-at-Sanford.com Mon Mar 16 11:56:13 2009
24, 34 -- Received: from mail96.messagelabs.com (mail96.messagelabs.com [216.82.254.19])
24, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2GGuCoZ003515
24, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 11:56:12 -0500
24, 34 -- X-VirusChecked: Checked
24, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
24, 34 -- X-Msg-Ref: server-9.tower-96.messagelabs.com!1237222568!52470382!1
24, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
24, 34 -- X-Originating-IP: [198.176.16.25]
24, 34 -- Received: (qmail 6375 invoked from network); 16 Mar 2009 16:56:11 -0000
24, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
24, 34 -- by server-9.tower-96.messagelabs.com with SMTP; 16 Mar 2009 16:56:11 -0000
24, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
24, 34 -- Mon, 16 Mar 2009 11:56:06 -0500
24, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 34 -- Content-class: urn:content-classes:message
24, 34 -- MIME-Version: 1.0
24, 34 -- Content-Type: text/plain;
24, 34 -- charset="US-ASCII"
24, 34 -- Subject: RE: [Microscopy] Light Microscopy
24, 34 -- Date: Mon, 16 Mar 2009 11:54:17 -0500
24, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402088517-at-naoaksasebe02.nr.ad.newellco.com}
24, 34 -- In-Reply-To: {200903161626.n2GGQHj4025322-at-ns.microscopy.com}
24, 34 -- X-MS-Has-Attach:
24, 34 -- X-MS-TNEF-Correlator:
24, 34 -- Thread-Topic: [Microscopy] Light Microscopy
24, 34 -- Thread-Index: AcmmU/BRt9VC/ZhITeagukCJyhvAQwAAS0Fw
24, 34 -- References: {200903161626.n2GGQHj4025322-at-ns.microscopy.com}
24, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
24, 34 -- To: {paul.gerroir-at-xrcc.xeroxlabs.com}
24, 34 -- Cc: {Microscopy-at-microscopy.com}
24, 34 -- X-OriginalArrivalTime: 16 Mar 2009 16:56:06.0260 (UTC) FILETIME=[18F1C340:01C9A658]
24, 34 -- Content-Transfer-Encoding: 8bit
24, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GGuCoZ003515
==============================End of - Headers==============================




From: pbozzano-at-cnea.gov.ar
Date: Mon, 16 Mar 2009 13:09:58 -0500
Subject: [Microscopy] CIASEM 2009

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues:
 
Hereby I inform you that due to the request of many colleagues:
 
The deadline of the CIASEM 2009 Pre-registration and Submission of Short
Abstracts has been extended to APRIL 15, 2009.
 
We encourage you to participate in this important event to promote the
collaboration towards the scientific and technological development of our
Nations. Please visit our web page www.ciasem2009.com.ar
 
On behalf of the organizing Committee CIASEM 2009

-----------------------------------------------
Dra. Patricia B.Bozzano
Comisión Nacional de Energía Atómica
Buenos Aires, Argentina
Tel 54 11 6772 7395 / 7282
Fax 54 11 6772 7740




==============================Original Headers==============================
5, 27 -- From pbozzano-at-cnea.gov.ar Mon Mar 16 13:09:56 2009
5, 27 -- Received: from cnea.gov.ar (diavolo.cnea.gov.ar [200.68.249.35])
5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GI9trO020361
5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 13:09:56 -0500
5, 27 -- Received: from cnea-mail.cnea.gov.ar (cnea-mail.cnea.gov.ar [168.96.68.244])
5, 27 -- by cnea.gov.ar (8.11.2/8.11.2) with ESMTP id n2GIBm528525
5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 15:11:48 -0300
5, 27 -- Received: from uam61 (uam061.cnea.gov.ar [192.168.16.62])
5, 27 -- by cnea-mail.cnea.gov.ar (8.12.10/8.12.10) with ESMTP id n2GIBU5w016807
5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 15:11:30 -0300
5, 27 -- From: "Patricia B.Bozzano" {pbozzano-at-cnea.gov.ar}
5, 27 -- To: {Microscopy-at-microscopy.com}
5, 27 -- Subject: CIASEM 2009
5, 27 -- Date: Mon, 16 Mar 2009 15:09:01 -0300
5, 27 -- Organization: C.N.E.A.
5, 27 -- Message-ID: {001601c9a662$490993a0$db1cbae0$-at-gov.ar}
5, 27 -- MIME-Version: 1.0
5, 27 -- Content-Type: text/plain;
5, 27 -- charset="iso-8859-1"
5, 27 -- X-Mailer: Microsoft Office Outlook 12.0
5, 27 -- Thread-Index: AcmmYkd7dyXexf8PQmCe4nPgo0bVEw==
5, 27 -- Content-Language: es-ar
5, 27 -- x-cr-hashedpuzzle: ByUf CJTr DPee ET29 Ef6b FL6x G4F1 G5Tq HIvt HZZz HgoT HhFU Hw4X IC8/ IGME JKyz;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{2A4D9FAB-A412-4EAE-AC73-F9939C0649B5};cABiAG8AegB6AGEAbgBvAEAAYwBuAGUAYQAuAGcAbwB2AC4AYQByAA==;Mon, 16 Mar 2009 18:08:59 GMT;QwBJAEEAUwBFAE0AIAAyADAAMAA5AA==
5, 27 -- x-cr-puzzleid: {2A4D9FAB-A412-4EAE-AC73-F9939C0649B5}
5, 27 -- X-RAVMilter-Version: 8.4.4(snapshot 20030410) (cnea-mail)
5, 27 -- Content-Transfer-Encoding: 8bit
5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GI9trO020361
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Mon, 16 Mar 2009 14:20:33 -0500
Subject: [Microscopy] JEOL Tabletop SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just out of curiosity, does anybody know what the price point on the
JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models
pricing, but I'm curious about this one. Also, has anybody used it
and compared it to the other two? I'm fairly familiar with both
Hitachi and FEI, but I haven't had a chance to test drive the JEOL
yet.

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
3, 31 -- From kraftpiano-at-gmail.com Mon Mar 16 14:20:32 2009
3, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.147])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GJKWdt004414
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:20:32 -0500
3, 31 -- Received: by qw-out-1920.google.com with SMTP id 14so1974878qwa.54
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 12:20:32 -0700 (PDT)
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
3, 31 -- :from:to:content-type:content-transfer-encoding;
3, 31 -- bh=NYqkjWTA/1cjInP32lUIU4VN+E2li56xQSyIcf5CyLw=;
3, 31 -- b=Q1ZFVjwsXvwn33Bol9MVLt4IyTg+nNKvP8f573yg1+PYYGNL88iRD36sZqXNrFIf5l
3, 31 -- v0lg7o8SVrDr8KHd9A/DKQcVuoFqFVXHdkYkZTEtLyrunabvKFKhnx9Cs7k9/rHGgQxV
3, 31 -- W/4EIFTp+fIdCUd0ZzyxVbHYfQGTJoWjSrMfs=
3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
3, 31 -- :content-transfer-encoding;
3, 31 -- b=iGa1lWGdBL6ljP9sZqf/V9YR17/277KkXAh6U4Mo6xn6fNPpiuITflrOSZL8U4oORQ
3, 31 -- IMpA62TF8aFN4XIxeITqIo8dNWr+JktOmCcGVXGxalcggbQXh5LQUr05+78V8zBXJ66v
3, 31 -- v88xnxVGMSADweZsUpgzuzykHFEIaARCiBXKI=
3, 31 -- MIME-Version: 1.0
3, 31 -- Received: by 10.224.54.76 with SMTP id p12mr6271346qag.207.1237231232379; Mon,
3, 31 -- 16 Mar 2009 12:20:32 -0700 (PDT)
3, 31 -- Date: Mon, 16 Mar 2009 15:20:32 -0400
3, 31 -- Message-ID: {25e2b0d20903161220p161de458n3915cf6786f2572b-at-mail.gmail.com}
3, 31 -- Subject: JEOL Tabletop SEM
3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
3, 31 -- To: microscopy-at-microscopy.com
3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
3, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: schoenthal-at-vadiodes.com
Date: Mon, 16 Mar 2009 14:28:25 -0500
Subject: [Microscopy] Re: JEOL Tabletop SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

I recently received a quote on the Nikon/JEOL for $60k. When I looked
at all three models around a year ago, it seemed to me that the Nikon/
JEOL had the best compromise of mag, chamber size and cost, but things
may have changed or I could have been mistaken. I have been following
the JEOL, but not the other two.

Any updates on the other two would be appreciated. Also, I can forward
along a quote and/or contact info offline if needed.

Thanks,

Gerhard

On Mar 16, 2009, at 15:22 , kraftpiano-at-gmail.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Just out of curiosity, does anybody know what the price point on the
} JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models
} pricing, but I'm curious about this one. Also, has anybody used it
} and compared it to the other two? I'm fairly familiar with both
} Hitachi and FEI, but I haven't had a chance to test drive the JEOL
} yet.
}
} --Justin A. Kraft
}
} --
} "America believes in education; the average professor earns more money
} in a year than a professional athlete earns in a whole week." Evan
} Esar


==============================Original Headers==============================
8, 20 -- From schoenthal-at-vadiodes.com Mon Mar 16 14:28:25 2009
8, 20 -- Received: from mail312.opentransfer.com (mail312.opentransfer.com [98.130.1.170])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2GJSPMO017165
8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:28:25 -0500
8, 20 -- Received: (qmail 1604 invoked by uid 399); 16 Mar 2009 19:28:24 -0000
8, 20 -- Received: from unknown (HELO ?10.1.10.235?) (75.150.52.245)
8, 20 -- by mail312.opentransfer.com with ESMTP; 16 Mar 2009 19:28:24 -0000
8, 20 -- X-Originating-IP: 75.150.52.245
8, 20 -- From: "Gerhard S. Schoenthal" {schoenthal-at-vadiodes.com}
8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 20 -- In-Reply-To: {200903161922.n2GJMbWa007342-at-ns.microscopy.com}
8, 20 -- Subject: Re: [Microscopy] JEOL Tabletop SEM
8, 20 -- References: {200903161922.n2GJMbWa007342-at-ns.microscopy.com}
8, 20 -- Message-Id: {52869867-18AD-489B-B267-5047A784A697-at-vadiodes.com}
8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 20 -- Content-Transfer-Encoding: 7bit
8, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3)
8, 20 -- Date: Mon, 16 Mar 2009 15:28:24 -0400
8, 20 -- Cc: kraftpiano-at-gmail.com
8, 20 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Mon, 16 Mar 2009 14:55:29 -0500
Subject: [Microscopy] JEOL Tabletop SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe the JEOL tabletop is quoted at $60K

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.



Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Monday, March 16, 2009 12:29 PM
To: dkloos-at-parallaxray.com

Just out of curiosity, does anybody know what the price point on the
JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models
pricing, but I'm curious about this one. Also, has anybody used it
and compared it to the other two? I'm fairly familiar with both
Hitachi and FEI, but I haven't had a chance to test drive the JEOL
yet.

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
3, 31 -- From kraftpiano-at-gmail.com Mon Mar 16 14:20:32 2009
3, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com
[74.125.92.147])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2GJKWdt004414
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:20:32
-0500
3, 31 -- Received: by qw-out-1920.google.com with SMTP id 14so1974878qwa.54
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 12:20:32
-0700 (PDT)
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 31 -- d=gmail.com; s=gamma;
3, 31 --
h=domainkey-signature:mime-version:received:date:message-id:subject
3, 31 -- :from:to:content-type:content-transfer-encoding;
3, 31 -- bh=NYqkjWTA/1cjInP32lUIU4VN+E2li56xQSyIcf5CyLw=;
3, 31 --
b=Q1ZFVjwsXvwn33Bol9MVLt4IyTg+nNKvP8f573yg1+PYYGNL88iRD36sZqXNrFIf5l
3, 31 --
v0lg7o8SVrDr8KHd9A/DKQcVuoFqFVXHdkYkZTEtLyrunabvKFKhnx9Cs7k9/rHGgQxV
3, 31 -- W/4EIFTp+fIdCUd0ZzyxVbHYfQGTJoWjSrMfs=
3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
3, 31 -- :content-transfer-encoding;
3, 31 --
b=iGa1lWGdBL6ljP9sZqf/V9YR17/277KkXAh6U4Mo6xn6fNPpiuITflrOSZL8U4oORQ
3, 31 --
IMpA62TF8aFN4XIxeITqIo8dNWr+JktOmCcGVXGxalcggbQXh5LQUr05+78V8zBXJ66v
3, 31 -- v88xnxVGMSADweZsUpgzuzykHFEIaARCiBXKI=
3, 31 -- MIME-Version: 1.0
3, 31 -- Received: by 10.224.54.76 with SMTP id
p12mr6271346qag.207.1237231232379; Mon,
3, 31 -- 16 Mar 2009 12:20:32 -0700 (PDT)
3, 31 -- Date: Mon, 16 Mar 2009 15:20:32 -0400
3, 31 -- Message-ID:
{25e2b0d20903161220p161de458n3915cf6786f2572b-at-mail.gmail.com}
3, 31 -- Subject: JEOL Tabletop SEM
3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
3, 31 -- To: microscopy-at-microscopy.com
3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
3, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================


==============================Original Headers==============================
12, 30 -- From dkloos-at-parallaxray.com Mon Mar 16 14:55:29 2009
12, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GJtSch000758
12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:55:28 -0500
12, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
12, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
12, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
12, 30 -- id 1LjIuM-000ACo-PV; Mon, 16 Mar 2009 13:55:34 -0600
12, 30 -- Reply-To: {dkloos-at-parallaxray.com}
12, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
12, 30 -- To: {kraftpiano-at-gmail.com}
12, 30 -- Cc: {microscopy-at-microscopy.com}
12, 30 -- References: {200903161928.n2GJSdCC017785-at-ns.microscopy.com}
12, 30 -- Subject: RE: [Microscopy] JEOL Tabletop SEM
12, 30 -- Date: Mon, 16 Mar 2009 12:55:22 -0700
12, 30 -- Organization: Parallax Research
12, 30 -- Message-ID: {AC5B2B67C8F24A0192AB7AE2C11F6BDB-at-donl}
12, 30 -- MIME-Version: 1.0
12, 30 -- Content-Type: text/plain;
12, 30 -- charset="us-ascii"
12, 30 -- Content-Transfer-Encoding: 7bit
12, 30 -- X-Mailer: Microsoft Office Outlook 11
12, 30 -- In-Reply-To: {200903161928.n2GJSdCC017785-at-ns.microscopy.com}
12, 30 -- Thread-Index: AcmmbW3EI6sIUVsOQwiQ8pKdz9MYqQAA6MHg
12, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
12, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
12, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
12, 30 -- X-AntiAbuse: Original Domain - microscopy.com
12, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
12, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Mon, 16 Mar 2009 17:08:43 -0500
Subject: [Microscopy] RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The symptoms vary with the degree of oxygen reduction. Also, some
acclimatization can take place. Everest has been climbed by a few people
without oxygen assist, but most people suddenly over 20,000 feet will
have many symptoms similar to being drunk, hence the origin of the
phrase "Getting High". The real danger is suddenly being in a situation
where sufficient oxygen has been depleted from the air to cause a lethal
situation. The fatality at Intel was caused when a technician started
fill of an LS 160, venting the gas into an enclosed area with no
automatic shut-off. He went to lunch, forgot about the dewar, and
several hours later as he re-entered the room alone, the spring-loaded
door closed behind him, sealing his doom. According to our safety
people, one deep breath of 100% nitrogen can cause you to pass out.
Similar situations occur tragically in workers repairing underground gas
pipelines. Leaking gas can displace 100% of the air, and workers lose
consciousness suddenly. Unfortunately, their would-be rescuers
frequently suffer the same consequence, resulting in multiple
fatalities. Another situation that the safety people were seriously
concerned about was our darkroom. It had multiple nitrogen hoses with
spring-loaded valves for dusting negatives prior to printing. Any
failure overnight or over a weekend of a hose or valve could result in
complete displacement of oxygen, and a technician entering the darkroom
through the rotating doors could be in a lethal situation.

John Mardinly,
Numonyx


-----Original Message-----
X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu]
Sent: Sunday, March 15, 2009 10:29 AM
To: MARDINLY, A

There have been a lot of comments on the dangers of hypoxia from
leaking or defective liquid nitrogen tanks. All very interesting.

QUESTION: Are there any straightforward symptoms of the onset of
hypoxia that can be recognized in time to avoid succumbing to the
situation??
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original
Headers==============================
2, 14 -- From bigelow-at-umich.edu Sun Mar 15 12:12:54 2009
2, 14 -- Received: from hellskitchen.mr.itd.umich.edu
(smtp.mail.umich.edu [141.211.14.82])
2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2FHCqTn025128
2, 14 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009
12:12:53 -0500
2, 14 -- Received: FROM [76.234.131.113]
(adsl-76-234-131-113.dsl.sfldmi.sbcglobal.net [76.234.131.113])
2, 14 -- BY hellskitchen.mr.itd.umich.edu ID 49BD3711.9A774.25996
;
2, 14 -- 15 Mar 2009 13:12:50 -0400
2, 14 -- Mime-Version: 1.0
2, 14 -- Message-Id: {p06240800c5e2e6aed43a-at-[99.130.26.88]}
2, 14 -- Date: Sun, 15 Mar 2009 13:12:46 -0400
2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
2, 14 -- From: Wil Bigelow {bigelow-at-umich.edu}
2, 14 -- Subject: [Microscopy] RE: symptoms of hypoxia
2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================



==============================Original Headers==============================
11, 29 -- From A.MARDINLY-at-numonyx.com Mon Mar 16 17:08:42 2009
11, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GM8gCo019245
11, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 17:08:42 -0500
11, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23])
11, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 869B4144024;
11, 29 -- Mon, 16 Mar 2009 16:10:39 -0400 (EDT)
11, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
11, 29 -- Mon, 16 Mar 2009 18:08:41 -0400
11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 29 -- Content-class: urn:content-classes:message
11, 29 -- MIME-Version: 1.0
11, 29 -- Content-Type: text/plain;
11, 29 -- charset="us-ascii"
11, 29 -- Subject: RE: [Microscopy] RE: symptoms of hypoxia
11, 29 -- Date: Mon, 16 Mar 2009 18:08:40 -0400
11, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F3A150-at-EXDRESBENMX012.numonyx.local}
11, 29 -- In-Reply-To: {200903151729.n2FHTGKm004498-at-ns.microscopy.com}
11, 29 -- X-MS-Has-Attach:
11, 29 -- X-MS-TNEF-Correlator:
11, 29 -- Thread-Topic: [Microscopy] RE: symptoms of hypoxia
11, 29 -- Thread-Index: Acmlk5Wyezl+sLUsTruME5wgVFj/NwA7dxlQ
11, 29 -- References: {200903151729.n2FHTGKm004498-at-ns.microscopy.com}
11, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
11, 29 -- To: {bigelow-at-umich.edu}
11, 29 -- Cc: {Microscopy-at-Microscopy.com}
11, 29 -- X-OriginalArrivalTime: 16 Mar 2009 22:08:41.0044 (UTC) FILETIME=[C3AAB140:01C9A683]
11, 29 -- Content-Transfer-Encoding: 8bit
11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GM8gCo019245
==============================End of - Headers==============================




From: hanke-at-mee-inc.com
Date: Mon, 16 Mar 2009 17:38:28 -0500
Subject: [Microscopy] RE: Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul:

I generally agree with Robert Zonis's comments. The issue of portability
is a major advantage of the Keyence and Hirox systems. The extended
depth of field functions on this instruments is also very good.

However, in my opinion, some of the newer, more traditional light
microscopes have superior image sharpness. We chose the Nikon AZ100 in
favor of the Keyence, partially for the ease of use and improved
sharpness for traditional bench microscopy. The Nikon extended depth of
field software is not so good - I would stick with the CZM or similar
freeware if this function is needed. The cost of the Nikon was also a
factor - about 1/3 that of the Keyence system with similar magnification
range. In the end, the best choice here will depend on how you will be
using the instrument. I am very happy with the Nikon, but would love to
have the Keyence or Hirox for some applications if I had the financial
resources.

Good luck.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

} Paul,
}
} Informally, we've had demonstrations here of several types of
} microscopes, including conventional microscopes (Nikon) and digital
} microscopes (Keyence).
}
} The three biggest differences we found were 1) Ease of capturing crisp,
} focused images at high to very high resolutions 2) the ability of the
} digital microscopes to generate images with substantial depth of field,
} due to on-the-fly processing of image stacks, and 3) the ability of the
} digital microscope to take an image at several angles, so that the
} software can produce a 3-d profile/image of the object. If you don't
} need the 3-d profiles, and can wait for image stacks to process, a
} conventional light microscope with a decent camera, software and
} motorized stage/focus would be a less expensive (but somewhat fuzzier)
} alternative.
}
} Compared to the Keyence system, you are also giving up some portability;
} I don't know how much that would mean to you.
}
} I have no connection to any of these companies.
}
} Robert Zonis
} Technical Service, LMTC
} Sanford L.P. - A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
}
} This message is intended for the Microscopy Listserv. Permission is
} specifically granted to the Microscopy Society of America to publish
} some or all of this message in the Microscopy Today journal.
}
}
} -----Original Message-----
} X-from: paul.gerroir-at-xrcc.xeroxlabs.com
} [mailto:paul.gerroir-at-xrcc.xeroxlabs.com]
} Sent: Monday, March 16, 2009 11:26 AM
} To: Zonis, Robert
} Subject: [Microscopy] Light Microscopy
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} All,
} I have been wondering if anyone has done a thorough comparison between a
} digital microscope, (Hirox, Keyence) and a conventional light microscope
} Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is
} one type more suited for given application(s)? What about cost?
} Portability?
}
} There wasn't much information in the archives.
}
} Paul J. Gerroir
}
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: 905-823-7091, ext.216
} FAX: 905-822-7022
} e-mail: paul.gerroir-at-xerox.com


==============================Original Headers==============================
7, 25 -- From hanke-at-mee-inc.com Mon Mar 16 17:38:28 2009
7, 25 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GMcSqJ001209
7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 17:38:28 -0500
7, 25 -- Received: (qmail 8381 invoked by uid 508); 16 Mar 2009 17:38:27 -0500
7, 25 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms
7, 25 -- (perlscan: 1.24-st-qms.
7, 25 -- Clear:RC:1(216.14.180.82):.
7, 25 -- Processed in 0.098374 secs); 16 Mar 2009 22:38:27 -0000
7, 25 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com
7, 25 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.098374 secs Process 8374)
7, 25 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82)
7, 25 -- by mail.namisolutions.com with SMTP; 16 Mar 2009 17:38:27 -0500
7, 25 -- Message-ID: {49BED4DD.2070401-at-mee-inc.com}
7, 25 -- Date: Mon, 16 Mar 2009 17:38:21 -0500
7, 25 -- From: Larry Hanke {hanke-at-mee-inc.com}
7, 25 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
7, 25 -- MIME-Version: 1.0
7, 25 -- To: microscopy-at-microscopy.com
7, 25 -- CC: paul.gerroir-at-xrcc.xeroxlabs.com
7, 25 -- Subject: RE: Light Microscopy
7, 25 -- References: {200903161656.n2GGuq4C004539-at-ns.microscopy.com}
7, 25 -- In-Reply-To: {200903161656.n2GGuq4C004539-at-ns.microscopy.com}
7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 25 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: arnec-at-bio.umass.edu
Date: Mon, 16 Mar 2009 18:43:18 -0500
Subject: [Microscopy] viaWWW: Looking for JEOL5800LV parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: arnec-at-bio.umass.edu
Name: Arne Christensen

Organization: USGS - Conte Anadromous Fish Research Laboratory

Title-Subject: [Filtered] Looking for JEOL5800LV parts.

Question: Dear Listeners,

Approximately one year ago, we acquired a used JEOL5800LV SEM.
According to the JEOL associate who came to survey the instrument,
the unit is missing some critical components; a variable pumping unit
for the low vacuum mode, a stage, and a converter. The variable
pumping unit is make/model specific, and no longer being
manufactured. So, I am on the look out for a replacement.

I'm wondering if anybody is aware of a JEOL5800LV SEM that is not
operational, and may be used for parts.

Sincerely,
Arne

Login Host: 159.189.36.78
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Mar 16 18:43:18 2009
9, 11 -- Received: from [10.66.38.245] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GNhGBR016583
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 18:43:17 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c5e49483626e-at-[10.66.38.245]}
9, 11 -- Date: Mon, 16 Mar 2009 18:43:15 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: arnec-at-bio.umass.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Looking for JEOL5800LV parts
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DennisH658-at-aol.com
Date: Mon, 16 Mar 2009 20:32:46 -0500
Subject: [Microscopy] RE: Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul:

It depends on what you are working with. I was quite impressed with some
aspects of a Keyence demonstration we had a couple years ago. But they have
never been able to duplicate some of the cross-polarized views I can routinely
get with an old traditional Nikon. (I work with paint coatings that are often a
mixture of transparent and opaque layers).

Dennis Hinks
PPG Industries,
Cleveland, Ohio

===================================================

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Paul:

I generally agree with Robert Zonis's comments. The issue of portability
is a major advantage of the Keyence and Hirox systems. The extended
depth of field functions on this instruments is also very good.

However, in my opinion, some of the newer, more traditional light
microscopes have superior image sharpness. We chose the Nikon AZ100 in
favor of the Keyence, partially for the ease of use and improved
sharpness for traditional bench microscopy. The Nikon extended depth of
field software is not so good - I would stick with the CZM or similar
freeware if this function is needed. The cost of the Nikon was also a
factor - about 1/3 that of the Keyence system with similar magnification
range. In the end, the best choice here will depend on how you will be
using the instrument. I am very happy with the Nikon, but would love to
have the Keyence or Hirox for some applications if I had the financial
resources.

Good luck.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870

} Paul,
}
} Informally, we've had demonstrations here of several types of
} microscopes, including conventional microscopes (Nikon) and digital
} microscopes (Keyence).
}
} The three biggest differences we found were 1) Ease of capturing crisp,
} focused images at high to very high resolutions 2) the ability of the
} digital microscopes to generate images with substantial depth of field,
} due to on-the-fly processing of image stacks, and 3) the ability of the
} digital microscope to take an image at several angles, so that the
} software can produce a 3-d profile/image of the object. If you don't
} need the 3-d profiles, and can wait for image stacks to process, a
} conventional light microscope with a decent camera, software and
} motorized stage/focus would be a less expensive (but somewhat fuzzier)
} alternative.
}
} Compared to the Keyence system, you are also giving up some portability;
} I don't know how much that would mean to you.
}
} I have no connection to any of these companies.
}
} Robert Zonis
} Technical Service, LMTC
} Sanford L.P. - A Newell Rubbermaid Company
} Shelbyville, TN 37160
} Direct: +1 (931) 685-6635
}
} This message is intended for the Microscopy Listserv. Permission is
} specifically granted to the Microscopy Society of America to publish
} some or all of this message in the Microscopy Today journal.
}
}
} -----Original Message-----
} X-from: paul.gerroir-at-xrcc.xeroxlabs.com
} [mailto:paul.gerroir-at-xrcc.xeroxlabs.com]
} Sent: Monday, March 16, 2009 11:26 AM
} To: Zonis, Robert
} Subject: [Microscopy] Light Microscopy
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} All,
} I have been wondering if anyone has done a thorough comparison between a
} digital microscope, (Hirox, Keyence) and a conventional light microscope
} Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is
} one type more suited for given application(s)? What about cost?
} Portability?
}
} There wasn't much information in the archives.
}
} Paul J. Gerroir
}
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: 905-823-7091, ext.216
} FAX: 905-822-7022
} e-mail: paul.gerroir-at-xerox.com

**************Feeling the pinch at the grocery store? Make meals for Under
$10. (http://food.aol.com/frugal-feasts?ncid=emlcntusfood00000002)

==============================Original Headers==============================
13, 17 -- From DennisH658-at-aol.com Mon Mar 16 20:32:46 2009
13, 17 -- Received: from imo-d05.mx.aol.com (imo-d05.mx.aol.com [205.188.157.37])
13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H1WkPP032185
13, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 20:32:46 -0500
13, 17 -- Received: from DennisH658-at-aol.com
13, 17 -- by imo-d05.mx.aol.com (mail_out_v39.1.) id w.c66.4bfff3aa (29672)
13, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 21:32:41 -0400 (EDT)
13, 17 -- From: DennisH658-at-aol.com
13, 17 -- Message-ID: {c66.4bfff3aa.36f057bd-at-aol.com}
13, 17 -- Date: Mon, 16 Mar 2009 21:32:45 EDT
13, 17 -- Subject: RE: Light Microscopy
13, 17 -- To: Microscopy-at-microscopy.com
13, 17 -- MIME-Version: 1.0
13, 17 -- Content-Type: text/plain; charset="US-ASCII"
13, 17 -- Content-Transfer-Encoding: 7bit
13, 17 -- X-Mailer: AOL 9.5 sub 29
13, 17 -- X-Spam-Flag: NO
==============================End of - Headers==============================




From: ph2-at-sprynet.com
Date: Mon, 16 Mar 2009 22:10:42 -0500
Subject: [Microscopy] FYI - Call for Papers: Inter/Micro 2009

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Inter/Micro 2009
July 6-10, 2009
Chicago, Illinois
Millennium Knickerbocker Hotel
Symposia

Deadline for titles and abstracts: April 15, 2009

Papers are being solicited in the following subjects:
- Photomicrography and Scientific Digital Imaging
- Microscopes (confocal, fluorescence, scanning tunneling, polarizing, etc.)
- White Powder and Bio Terrorism Threats
- Resources, Books, Atlases, Databases
- Historical Topics
- Criminalistics, Forensic Microscopy & Trace Evidence (fibers, explosives,
paint, glass, drugs, inks, etc.)
- Art Conservation and Authentication
- Pharmaceuticals
- Environmental and Hazardous Dusts, Aerobiology (asbestos, mold, fungal
spores, indoor air quality)
- Geographical Sourcing
- Teaching Microscopy/Education
- Microscopy Tricks of the Trade
- Micro-Analytical Methods (SEM/EDS, TEM, FTIR, Raman)
- Other: Industrial Microscopy, Crystallography, Mineralogy

Go to:

http://www.mcri.org/home/section/101-399-409/call-for-papers%3A-inter-micro-
2009



Tony


......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
www.ph2llc.com

(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.




==============================Original Headers==============================
14, 26 -- From ph2-at-sprynet.com Mon Mar 16 22:10:42 2009
14, 26 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69])
14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H3AgEj018271
14, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 22:10:42 -0500
14, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 26 -- s=dk20050327; d=sprynet.com;
14, 26 -- b=tmJy3GKYIac7iqiOHtXYrKi2PHh+a87O2cQFn/BzDew+oMFYsOwiHGvUanseIfIj;
14, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP;
14, 26 -- Received: from [99.50.135.158] (helo=user915fa8f284)
14, 26 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
14, 26 -- (envelope-from {ph2-at-sprynet.com} )
14, 26 -- id 1LjPhR-0000FB-I1; Mon, 16 Mar 2009 23:10:41 -0400
14, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
14, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com}
14, 26 -- Subject: FYI - Call for Papers: Inter/Micro 2009
14, 26 -- Date: Mon, 16 Mar 2009 23:10:30 -0400
14, 26 -- MIME-Version: 1.0
14, 26 -- Content-Type: text/plain;
14, 26 -- charset="us-ascii"
14, 26 -- Content-Transfer-Encoding: 7bit
14, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
14, 26 -- Thread-Index: Acmmre1jyWSZlS9FTyC3BDZv9UvEtg==
14, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
14, 26 -- Message-ID: {E1LjPhR-0000FB-I1-at-elasmtp-mealy.atl.sa.earthlink.net}
14, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9bd2dc4292b5adcb291b26e9375db49d6350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
14, 26 -- X-Originating-IP: 99.50.135.158
==============================End of - Headers==============================




From: john.mitchels-at-gmail.com
Date: Tue, 17 Mar 2009 03:49:43 -0500
Subject: [Microscopy] Wanted: Parts for Jeol 1200 EX (MK1/2)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All
Does anyone know where we can get hold op parts for Jeol 1200's
(mk1/2) TEM. We have been infomed by Jeol that many of the the parts
are obselete but I am sure they must be available out there some
where.
Regards
John

==============================Original Headers==============================
1, 31 -- From john.mitchels-at-gmail.com Tue Mar 17 03:49:43 2009
1, 31 -- Received: from mail-bw0-f160.google.com (mail-bw0-f160.google.com [209.85.218.160])
1, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H8ng3T016880
1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 03:49:42 -0500
1, 31 -- Received: by bwz4 with SMTP id 4so4016756bwz.18
1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 01:49:41 -0700 (PDT)
1, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
1, 31 -- :from:to:content-type:content-transfer-encoding;
1, 31 -- bh=rYiO4l2vF7rkwJQ+AWKjBcT0tF6/8PVUWAvquHY7jIM=;
1, 31 -- b=IiqTpg9iswTadjjGHjAgqM1NREUg0gkOr+OYfiw7Ryf4+776TYl1JaHBwivoK9ZqGk
1, 31 -- VrxM4fsaM7jKLf147ZAOnzbUeV1zAyl5XEVMF2j7jlCBx3lgATmG8P0TYxyftujEOIyI
1, 31 -- zjQiaM/yMRHDnHbibcxdspoI928vKGv3OXKv4=
1, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
1, 31 -- :content-transfer-encoding;
1, 31 -- b=Krfmbk3Rs5FZo7eNYUTxmAuqRaq5553ObJIfUiaKBX3JuGrSGy96HsMJ6s1vKIWQxQ
1, 31 -- Nskgegb+444JcH1HLGWNpdgmbazSGlayWR8273y2T97N4/hpxhZ/Stvp3gfPUBgditN1
1, 31 -- h+1lThhVIb/+CXvxGjIO3GTWcM0LB96HlBmtA=
1, 31 -- MIME-Version: 1.0
1, 31 -- Received: by 10.204.115.139 with SMTP id i11mr1955182bkq.199.1237279781784;
1, 31 -- Tue, 17 Mar 2009 01:49:41 -0700 (PDT)
1, 31 -- Date: Tue, 17 Mar 2009 08:49:41 +0000
1, 31 -- Message-ID: {1b3cf7c30903170149g76841a99o84174bd3ea88042e-at-mail.gmail.com}
1, 31 -- Subject: Wanted: Parts for Jeol 1200 EX (MK1/2)
1, 31 -- From: John Mitchels {john.mitchels-at-gmail.com}
1, 31 -- To: Microscopy-at-microscopy.com
1, 31 -- Content-Type: text/plain; charset=ISO-8859-1
1, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 17 Mar 2009 05:35:22 -0500
Subject: [Microscopy] FW: re: LN2 in confined space

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Although I suppose O2 monitors in liquid N2 tank storage rooms aren't much
use if your PhD student draws off some and carries a large dewar into a
small lift and promptly spills it there.

Actually this thread is useful, I regularly go into the our tissue/cell
cryo-storage area filled with many very large tanks of N2, and promptly put
my head into the top [vented] well of the N2 tanks trying in vain to find my
rack of samples amongst the many ice covered ones there. And I can't
actually remember giving the O2 monitor reading on the wall more than a
cursory glance recently [it's generally very boring and always says the same
thing], I just head for the samples - I guess I'm vaguely assuming an alarm
would ring anyway if the O2 concentration falls, and that the room
ventilation rate is fine [has been every visit so far], and I often go in
chatting with a colleague which can be distracting.....but from now on..

Actually, I have been victim to gas heater carbon monoxide poisoning while
in my student dive [age 22], and no, other than feeling lousy [thought I had
flu] I didn't notice the high CO gas levels at all [being odourless and all,
just like N2]. My mates parents suffered similarly years earlier while
watching TV, and only survived because the gas fire's pay-as-you-go gas
meter ran out of money and switched off the gas fire - they woke up at 4.00
in the morning. I survived because my attempts at draft exclusion in my
bedsit weren't entirely successful. Presumably CO poisoning is like a lack
of O2 as it blocks the O2 receptors in haemoglobin [but unlike cyanide, its
reversible if you happen to have some pure oxygen about].

Keith

http://www.carbonmonoxidekills.com/


-----------------------------------------------------------------

Dr Keith J Morris
Molecular Cytogenetics and Microscopy Core
The Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford
OX3 7BN
United Kingdom

Tel: +44 ( 0 ) 1865 287568
Email: kjmorris-at-well.ox.ac.uk
HomePage: http://www.well.ox.ac.uk/cytogenetics

}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Folks -
}
} Personally, I would like this thread to
} turn away from "hypoxia", the history,
} or symptoms.
}
} The MSDS, fire code, etc.... clearly state
} "do not store LN2 is confined spaces". Additionally,
} there must be excellent ventilation.
}
} Some suggest to wear a self-contained breathing
} apparatus if the oxygen content routinely falls
} below 19%.
}
} In New York City, you cannot legally keep a dewar
} within a few feet of a door or exit. Infact, it
} is illegal there to store LN2 is a public hallway.
}
} Hence, let us be preventive. An oxygen monitor from
} MSA (Mine Safety Appliances) can be bought from
} Lab Safety Supply, Fisher Sci, etc... for just $200.
}
} regards,
}
} JQuinn
}
}
}
} ==============================Original
} Headers==============================
} 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009
} 11, 12 -- Received: from www.matscieng.sunysb.edu
} (www.matscieng.sunysb.edu [129.49.36.33])
} 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2FIxLPu022978
} 11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21
} -0500
} 11, 12 -- Received: (from jquinn-at-localhost)
} 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350
} 11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53
-0500
} 11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500
} 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
} 11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu}
} 11, 12 -- To: microscopy-at-microscopy.com
} 11, 12 -- Subject: re: LN2 in confined space
} ==============================End of -
} Headers==============================
}








==============================Original Headers==============================
17, 20 -- From kjmorris-at-well.ox.ac.uk Tue Mar 17 05:35:21 2009
17, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HAZLZR001890
17, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 05:35:21 -0500
17, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
17, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
17, 20 -- id 1LjWdk-00013n-Jj
17, 20 -- for microscopy-at-microscopy.com; Tue, 17 Mar 2009 10:35:20 +0000
17, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
17, 20 -- To: {microscopy-at-microscopy.com}
17, 20 -- Subject: FW: [Microscopy] re: LN2 in confined space
17, 20 -- Date: Tue, 17 Mar 2009 10:35:20 -0000
17, 20 -- Message-ID: {4F3698AC996149FEB9602101FCA7ADDC-at-CytoWhizz}
17, 20 -- MIME-Version: 1.0
17, 20 -- Content-Type: text/plain;
17, 20 -- charset="us-ascii"
17, 20 -- Content-Transfer-Encoding: 7bit
17, 20 -- X-Mailer: Microsoft Office Outlook 11
17, 20 -- Thread-Index: AcmlzbMxrrQS/gZVSVeALuPG3QKlcwAU40yw
17, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
==============================End of - Headers==============================




From: ph2-at-sprynet.com
Date: Tue, 17 Mar 2009 08:06:51 -0500
Subject: [Microscopy] RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This looks like it might be a good topic for a paper.

Let me outline a few things:

Effects and symptoms (by % or ppm) of too low of O2
(easy table that I already have; also altitude data and partial
pressures)

Effects and symptoms (by % or ppm) of too high of N2
(most data is on divers including nitrogen narcosis)

Effects and symptoms (by % or ppm) of too high CO2
(best data is on submarine occupants, good opportunity to discuses
short term limits)

Effects and symptoms (by % or ppm) of too high of O2
(best data on rats at Navy Toxic Lab at Wright-Pat based on post
Apollo failure testing but some human; I'll see if this is releasable)

Good opportunity to discuss air exchange rates, decay, and time to re-entry
(I did some work on this for Dams that use CO2 cylinders to flood the
facility when an electrical fire occurs, just have to find it)


I could also discuss certain refrigerants (HCFCs for instance) that have
cardiac sensitization issues at high ppm (5-80,000 ppm [0.5-8%]) upon
release.


Would y'all be interested in an article?


Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
www.ph2llc.com

(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.


-----Original Message-----
X-from: A.MARDINLY-at-numonyx.com [mailto:A.MARDINLY-at-numonyx.com]
Sent: Monday, March 16, 2009 6:13 PM
To: ph2-at-sprynet.com

The symptoms vary with the degree of oxygen reduction. Also, some
acclimatization can take place. Everest has been climbed by a few people
without oxygen assist, but most people suddenly over 20,000 feet will
have many symptoms similar to being drunk, hence the origin of the
phrase "Getting High". The real danger is suddenly being in a situation
where sufficient oxygen has been depleted from the air to cause a lethal
situation. The fatality at Intel was caused when a technician started
fill of an LS 160, venting the gas into an enclosed area with no
automatic shut-off. He went to lunch, forgot about the dewar, and
several hours later as he re-entered the room alone, the spring-loaded
door closed behind him, sealing his doom. According to our safety
people, one deep breath of 100% nitrogen can cause you to pass out.
Similar situations occur tragically in workers repairing underground gas
pipelines. Leaking gas can displace 100% of the air, and workers lose
consciousness suddenly. Unfortunately, their would-be rescuers
frequently suffer the same consequence, resulting in multiple
fatalities. Another situation that the safety people were seriously
concerned about was our darkroom. It had multiple nitrogen hoses with
spring-loaded valves for dusting negatives prior to printing. Any
failure overnight or over a weekend of a hose or valve could result in
complete displacement of oxygen, and a technician entering the darkroom
through the rotating doors could be in a lethal situation.

John Mardinly,
Numonyx


-----Original Message-----
X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu]
Sent: Sunday, March 15, 2009 10:29 AM
To: MARDINLY, A

There have been a lot of comments on the dangers of hypoxia from
leaking or defective liquid nitrogen tanks. All very interesting.

QUESTION: Are there any straightforward symptoms of the onset of
hypoxia that can be recognized in time to avoid succumbing to the
situation??
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original
Headers==============================
2, 14 -- From bigelow-at-umich.edu Sun Mar 15 12:12:54 2009
2, 14 -- Received: from hellskitchen.mr.itd.umich.edu
(smtp.mail.umich.edu [141.211.14.82])
2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2FHCqTn025128
2, 14 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009
12:12:53 -0500
2, 14 -- Received: FROM [76.234.131.113]
(adsl-76-234-131-113.dsl.sfldmi.sbcglobal.net [76.234.131.113])
2, 14 -- BY hellskitchen.mr.itd.umich.edu ID 49BD3711.9A774.25996
;
2, 14 -- 15 Mar 2009 13:12:50 -0400
2, 14 -- Mime-Version: 1.0
2, 14 -- Message-Id: {p06240800c5e2e6aed43a-at-[99.130.26.88]}
2, 14 -- Date: Sun, 15 Mar 2009 13:12:46 -0400
2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
2, 14 -- From: Wil Bigelow {bigelow-at-umich.edu}
2, 14 -- Subject: [Microscopy] RE: symptoms of hypoxia
2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================



==============================Original Headers==============================
11, 29 -- From A.MARDINLY-at-numonyx.com Mon Mar 16 17:08:42 2009
11, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net
(mail1.numonyx.com [57.77.12.37])
11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2GM8gCo019245
11, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 17:08:42
-0500
11, 29 -- Received: from exdresfenmx02.numonyx.local (unknown
[10.96.252.23])
11, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP
id 869B4144024;
11, 29 -- Mon, 16 Mar 2009 16:10:39 -0400 (EDT)
11, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by
exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
11, 29 -- Mon, 16 Mar 2009 18:08:41 -0400
11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 29 -- Content-class: urn:content-classes:message
11, 29 -- MIME-Version: 1.0
11, 29 -- Content-Type: text/plain;
11, 29 -- charset="us-ascii"
11, 29 -- Subject: RE: [Microscopy] RE: symptoms of hypoxia
11, 29 -- Date: Mon, 16 Mar 2009 18:08:40 -0400
11, 29 -- Message-ID:
{21B544109D3D3E4380B776AC7CEA8CF9F3A150-at-EXDRESBENMX012.numonyx.local}
11, 29 -- In-Reply-To: {200903151729.n2FHTGKm004498-at-ns.microscopy.com}
11, 29 -- X-MS-Has-Attach:
11, 29 -- X-MS-TNEF-Correlator:
11, 29 -- Thread-Topic: [Microscopy] RE: symptoms of hypoxia
11, 29 -- Thread-Index: Acmlk5Wyezl+sLUsTruME5wgVFj/NwA7dxlQ
11, 29 -- References: {200903151729.n2FHTGKm004498-at-ns.microscopy.com}
11, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
11, 29 -- To: {bigelow-at-umich.edu}
11, 29 -- Cc: {Microscopy-at-Microscopy.com}
11, 29 -- X-OriginalArrivalTime: 16 Mar 2009 22:08:41.0044 (UTC)
FILETIME=[C3AAB140:01C9A683]
11, 29 -- Content-Transfer-Encoding: 8bit
11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2GM8gCo019245
==============================End of - Headers==============================


==============================Original Headers==============================
33, 28 -- From ph2-at-sprynet.com Tue Mar 17 08:06:50 2009
33, 28 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69])
33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HD6oOZ019989
33, 28 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 08:06:50 -0500
33, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
33, 28 -- s=dk20050327; d=sprynet.com;
33, 28 -- b=UbnDQHab5DRAWGYmawEK/jb9z0WKxf0vPWp+kzXrVFDhdmXlSlJtt8LoPa8X29lz;
33, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:In-Reply-To:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP;
33, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284)
33, 28 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
33, 28 -- (envelope-from {ph2-at-sprynet.com} )
33, 28 -- id 1LjZ0L-0003qc-7q; Tue, 17 Mar 2009 09:06:49 -0400
33, 28 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
33, 28 -- To: {A.MARDINLY-at-numonyx.com} ,
33, 28 -- "Microscopy Listserve" {microscopy-at-microscopy.com}
33, 28 -- Subject: RE: [Microscopy] RE: RE: symptoms of hypoxia
33, 28 -- Date: Tue, 17 Mar 2009 09:06:38 -0400
33, 28 -- MIME-Version: 1.0
33, 28 -- Content-Type: text/plain;
33, 28 -- charset="us-ascii"
33, 28 -- Content-Transfer-Encoding: 7bit
33, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
33, 28 -- Thread-Index: AcmmhHLgbVpla2ELTC24hwS8QiW6ZwAfJQiw
33, 28 -- In-Reply-To: {200903162213.n2GMDMvA026464-at-ns.microscopy.com}
33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
33, 28 -- Message-ID: {E1LjZ0L-0003qc-7q-at-elasmtp-mealy.atl.sa.earthlink.net}
33, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f91331076f26a8a30b5fa6a0626caa7495350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
33, 28 -- X-Originating-IP: 75.61.18.94
==============================End of - Headers==============================




From: pveril-at-med.uth.gr
Date: Tue, 17 Mar 2009 10:34:28 -0500
Subject: [Microscopy] viaWWW: Cambridge stereoscan 240 (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pveril-at-med.uth.gr as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pveril-at-med.uth.gr
Name: Panos Verillis

Organization: Univercity of Thessaly

Title-Subject: [Filtered] Cambridge stereoscan 240 (SEM)

Question: I have a problem with a stereoscan 240 (Cambridge). When i
turn it on, the vacuum led lights on (then the microscope is ready
for the high voltage) and i press the operate button i had no raster
on the screen. Also the beam led does not light on (neither the fail,
or the trip). Does any one know what is the problem and how can i
correct it?

Thank you

Login Host: 195.251.17.161
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Mar 17 10:34:27 2009
7, 11 -- Received: from [10.71.0.118] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HFYP2N006176
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 10:34:27 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c5e57371873c-at-[10.71.0.118]}
7, 11 -- Date: Tue, 17 Mar 2009 10:34:24 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: pveril-at-med.uth.gr (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Cambridge stereoscan 240 (SEM)
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Tue, 17 Mar 2009 11:04:48 -0500
Subject: [Microscopy] Re: FW: re: LN2 in confined space

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Large dewars of liquid nitrogen should never ride an elevator with
people in it. On the UC Berkeley campus and at the Lawrence Berkeley
Lab large LN2 dewars ride the elevator alone with a large sign warning
not to get on with it. The researcher takes a different elevator or
runs the stairs.

Roseann


Roseann Csencsits, PhD
Scientist in Charge - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548







On Mar 17, 2009, at 3:42 AM, kjmorris-at-well.ox.ac.uk wrote:

}
} Although I suppose O2 monitors in liquid N2 tank storage rooms
} aren't much
} use if your PhD student draws off some and carries a large dewar
} into a
} small lift and promptly spills it there.


==============================Original Headers==============================
13, 26 -- From RCsencsits-at-lbl.gov Tue Mar 17 11:04:46 2009
13, 26 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HG4hCj021128
13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 11:04:45 -0500
13, 26 -- X-Ironport-SBRS: 2.3
13, 26 -- X-IronPort-Anti-Spam-Filtered: true
13, 26 -- X-IronPort-Anti-Spam-Result: AucCAFdmv0mAAykYe2dsb2JhbACVWQEBFiIFr2EJjzOCSYEzBmGGNg
13, 26 -- X-IronPort-AV: E=Sophos;i="4.38,379,1233561600";
13, 26 -- d="scan'208";a="97188986"
13, 26 -- Received: from mta1.lbl.gov ([128.3.41.24])
13, 26 -- by ironport2.lbl.gov with ESMTP; 17 Mar 2009 09:04:42 -0700
13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246])
13, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n2HG4fhD001121;
13, 26 -- Tue, 17 Mar 2009 09:04:41 -0700 (PDT)
13, 26 -- Cc: Microscopy-at-microscopy.com
13, 26 -- Message-Id: {E16E186E-E62F-407B-BDDD-E512B8A60B8C-at-lbl.gov}
13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
13, 26 -- To: kjmorris-at-well.ox.ac.uk
13, 26 -- In-Reply-To: {200903171042.n2HAgXDX012322-at-ns.microscopy.com}
13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 26 -- Content-Transfer-Encoding: 7bit
13, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3)
13, 26 -- Subject: Re: [Microscopy] FW: re: LN2 in confined space
13, 26 -- Date: Tue, 17 Mar 2009 07:20:19 -0700
13, 26 -- References: {200903171042.n2HAgXDX012322-at-ns.microscopy.com}
13, 26 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 17 Mar 2009 11:49:55 -0500
Subject: [Microscopy] Re: RE: symptoms of hypoxia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 17, 2009, at 6:06 AM, ph2-at-sprynet.com wrote:

} Would y'all be interested in an article?


Dear Tony,
I'd be interested.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Tue Mar 17 11:49:55 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HGnsfb003813
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 11:49:55 -0500
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 4E1BC2E50C72
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 09:49:53 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 60F902E50C6E
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 09:49:52 -0700 (PDT)
6, 22 -- Message-Id: {96948574-53EB-47BD-8DB8-BA4538E205BA-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200903171306.n2HD6w57020113-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia
6, 22 -- Date: Tue, 17 Mar 2009 09:49:51 -0700
6, 22 -- References: {200903171306.n2HD6w57020113-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: r.gilbert-at-auckland.ac.nz
Date: Tue, 17 Mar 2009 15:41:03 -0500
Subject: [Microscopy] FW: FW: re: LN2 in confined space

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Of course people are often injured by not following the written safety
procedures, hence my comment about an inexperienced young PhD student in a
hurry. Safety interlocks bypassed, category IV lasers operated without any
shielding or eye protection, burners fired up without a flame trap.. etc...
and I know I haven't seen it all.

That said health & Safety is far superior these days compared to when I
started my PhD in fuel and combustion back in the late 1970s [two people
died in that department while I was there - one senior lecturer killed by
carbon monoxide poisoning from a gas cylinder, the other a PhD student
killed by a gas meter explosion [no flame trap fitted]. I carried on using
that same carbon monoxide gas cylinder, with one modification - a jubilee
clip was fitted, clamping the clear plastic tubing to the pressure regulator
to 'prevent' it slipping off again.

Our Oxford Universities Health & Safety POLICY STATEMENT S4/03 hopefully
says it all about using liquid nitrogen safely:
http://www.admin.ox.ac.uk/safety/s403.shtml

Keith


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


-----Original Message-----
X-from: Roseann Csencsits [mailto:RCsencsits-at-lbl.gov]
Sent: 17 March 2009 14:20
To: kjmorris-at-well.ox.ac.uk
Cc: Microscopy-at-Microscopy.Com

You can reverse cyanide poisoning as well, you just need amyl nitrate. This is routinely carried by all sensible possum trappers here in New Zealand to prevent accidental poisoning when in the wilds.


Presumably CO poisoning is like a lack
of O2 as it blocks the O2 receptors in haemoglobin [but unlike cyanide, its
reversible if you happen to have some pure oxygen about].



Cheers
 
Ray G


.

http://www.carbonmonoxidekills.com/


-----------------------------------------------------------------

Dr Keith J Morris
Molecular Cytogenetics and Microscopy Core
The Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford
OX3 7BN
United Kingdom

Tel: +44 ( 0 ) 1865 287568
Email: kjmorris-at-well.ox.ac.uk
HomePage: http://www.well.ox.ac.uk/cytogenetics

}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Folks -
}
} Personally, I would like this thread to
} turn away from "hypoxia", the history,
} or symptoms.
}
} The MSDS, fire code, etc.... clearly state
} "do not store LN2 is confined spaces". Additionally,
} there must be excellent ventilation.
}
} Some suggest to wear a self-contained breathing
} apparatus if the oxygen content routinely falls
} below 19%.
}
} In New York City, you cannot legally keep a dewar
} within a few feet of a door or exit. Infact, it
} is illegal there to store LN2 is a public hallway.
}
} Hence, let us be preventive. An oxygen monitor from
} MSA (Mine Safety Appliances) can be bought from
} Lab Safety Supply, Fisher Sci, etc... for just $200.
}
} regards,
}
} JQuinn
}
}
}
} ==============================Original
} Headers==============================
} 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009
} 11, 12 -- Received: from www.matscieng.sunysb.edu
} (www.matscieng.sunysb.edu [129.49.36.33])
} 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2FIxLPu022978
} 11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21
} -0500
} 11, 12 -- Received: (from jquinn-at-localhost)
} 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350
} 11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53
-0500
} 11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500
} 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
} 11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu}
} 11, 12 -- To: microscopy-at-microscopy.com
} 11, 12 -- Subject: re: LN2 in confined space
} ==============================End of -
} Headers==============================
}








==============================Original Headers==============================
17, 20 -- From kjmorris-at-well.ox.ac.uk Tue Mar 17 05:35:21 2009
17, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HAZLZR001890
17, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 05:35:21 -0500
17, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
17, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
17, 20 -- id 1LjWdk-00013n-Jj
17, 20 -- for microscopy-at-microscopy.com; Tue, 17 Mar 2009 10:35:20 +0000
17, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
17, 20 -- To: {microscopy-at-microscopy.com}
17, 20 -- Subject: FW: [Microscopy] re: LN2 in confined space
17, 20 -- Date: Tue, 17 Mar 2009 10:35:20 -0000
17, 20 -- Message-ID: {4F3698AC996149FEB9602101FCA7ADDC-at-CytoWhizz}
17, 20 -- MIME-Version: 1.0
17, 20 -- Content-Type: text/plain;
17, 20 -- charset="us-ascii"
17, 20 -- Content-Transfer-Encoding: 7bit
17, 20 -- X-Mailer: Microsoft Office Outlook 11
17, 20 -- Thread-Index: AcmlzbMxrrQS/gZVSVeALuPG3QKlcwAU40yw
17, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
==============================End of - Headers==============================


==============================Original Headers==============================
22, 31 -- From r.gilbert-at-auckland.ac.nz Tue Mar 17 15:41:03 2009
22, 31 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35])
22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HKeuXE006228
22, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 15:41:02 -0500
22, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1])
22, 31 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 65FCC482754
22, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:40:54 +1300 (NZDT)
22, 31 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
22, 31 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
22, 31 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
22, 31 -- with ESMTP id Jt+FVScm-WpK for {Microscopy-at-microscopy.com} ;
22, 31 -- Wed, 18 Mar 2009 09:40:54 +1300 (NZDT)
22, 31 -- Received: from fmhsx1.fmhs.auckland.ac.nz (fmhsx1.fmhs.auckland.ac.nz [130.216.128.61])
22, 31 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1A39C4826C2
22, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:40:54 +1300 (NZDT)
22, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
22, 31 -- Content-class: urn:content-classes:message
22, 31 -- MIME-Version: 1.0
22, 31 -- Content-Type: text/plain;
22, 31 -- charset="iso-8859-1"
22, 31 -- Subject: FW: [Microscopy] FW: re: LN2 in confined space
22, 31 -- Date: Wed, 18 Mar 2009 09:40:06 +1300
22, 31 -- Message-ID: {738FC0785373EC41B3B88A65CFBC981501C8B37C-at-FMHSX1.fmhs.auckland.ac.nz}
22, 31 -- X-MS-Has-Attach:
22, 31 -- X-MS-TNEF-Correlator:
22, 31 -- Thread-Topic: [Microscopy] FW: re: LN2 in confined space
22, 31 -- thread-index: Acmm7O8kWxGYjNweSiufdThtmXWC+QAUzLGg
22, 31 -- From: "Ray Gilbert" {r.gilbert-at-auckland.ac.nz}
22, 31 -- To: {Microscopy-at-microscopy.com}
22, 31 -- Content-Transfer-Encoding: 8bit
22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2HKeuXE006228
==============================End of - Headers==============================




From: schooley-at-mcn.org
Date: Tue, 17 Mar 2009 16:12:33 -0500
Subject: [Microscopy] Microscopy education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This has just appeared in a Sigma Xi Email newsletter:

"PUBLISHER LOOKING FOR CHILDREN'S BOOKS ON SCIENCE Magic World Media
is a new children's book publishing company focused on publishing
imaginative picture books and early chapter books on scientific
topics that extend the world view of children beyond their sensory
experience and introduce them to the vastness of what is still
unknown. Their first titles will be launched in the fall of this
year. They are now actively seeking manuscripts for publication in
2010 and beyond on various topics and are particularly interested in
the topics of dark matter and light. Visit
http://www.magicworldmedia.com to view submission guidelines. To be
considered for publication in 2010, picture book manuscripts must be
received by May 31, 2009, and chapter book manuscripts by October 31."
--
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
2, 16 -- From schooley-at-mcn.org Tue Mar 17 16:12:32 2009
2, 16 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32])
2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HLCTsA020572
2, 16 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Mar 2009 16:12:30 -0500
2, 16 -- Received: from [66.81.65.112] (helo=[66.81.64.22])
2, 16 -- by dns3.mcn.org with esmtpa (Exim 4.69)
2, 16 -- (envelope-from {schooley-at-mcn.org} )
2, 16 -- id 1LjgaI-0008Nd-3d
2, 16 -- for Microscopy-at-Microscopy.Com; Tue, 17 Mar 2009 14:12:27 -0700
2, 16 -- Mime-Version: 1.0
2, 16 -- Message-Id: {a06200707c5e5c495b53c-at-[66.81.64.22]}
2, 16 -- Date: Tue, 17 Mar 2009 14:23:10 -0700
2, 16 -- To: Microscopy-at-Microscopy.Com
2, 16 -- From: Caroline Schooley {schooley-at-mcn.org}
2, 16 -- Subject: Microscopy education
2, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: RHsia-at-umaryland.edu
Date: Tue, 17 Mar 2009 23:01:42 -0500
Subject: [Microscopy] instrument comparison

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I know many of you are probably in the process of submitting equipment grant to acquire new instrument due to the stimulus package (I come in peace, my fellow competitors!). I wonder if any of you are willing to share your experience in using RMC cryo-ultramicrotome, particularly the pros and cons of the RMC cryo-ultramicrotome compared to Leica UC6 and FC6. I am also very interested to know any opinion about RMC freeze substitution machine (FS7500) and its comparison to Leica AFS2. I appreciate any feedback/comment if you have used any of these instruments. Thank you so much.

If you are concerned about conflict of interest, please rely to my personal e-mail.

Thank you very much.

Sincerely,

Ru-ching


Ru-ching Hsia, Ph.D  
Director, Core Imaging Facility
http://www.dental.umaryland.edu/Core-imaging
E-mail: rhsia-at-umaryland.edu




==============================Original Headers==============================
10, 22 -- From RHsia-at-umaryland.edu Tue Mar 17 23:01:42 2009
10, 22 -- Received: from cits-exch1.campus.umaryland.edu (cits-exch1.campus.umaryland.edu [134.192.1.123])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2I41gGa011069
10, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 23:01:42 -0500
10, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 22 -- MIME-Version: 1.0
10, 22 -- Content-Type: text/plain;
10, 22 -- charset="UTF-8"
10, 22 -- x-cr-puzzleid: {A3812D72-9703-49FA-A57D-4D38E947C91C}
10, 22 -- Content-class: urn:content-classes:message
10, 22 -- x-cr-hashedpuzzle: AG2e Ajyf Ay77 DPSz F89o HHlv Htsh Hy8O IPMa IXC/ JB1x JJRA JtVT J28B KGwO LYXS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{A3812D72-9703-49FA-A57D-4D38E947C91C};cgBoAHMAaQBhAEAAdQBtAGEAcgB5AGwAYQBuAGQALgBlAGQAdQA=;Wed, 18 Mar 2009 04:01:46 GMT;aQBuAHMAdAByAHUAbQBlAG4AdAAgAGMAbwBtAHAAYQByAGkAcwBvAG4A
10, 22 -- Subject: instrument comparison
10, 22 -- Date: Wed, 18 Mar 2009 00:01:46 -0400
10, 22 -- Message-ID: {3BBD3EDE51DB2043ABF3E819B1CD532A08FA1B5F-at-cits-exch1.campus.umaryland.edu}
10, 22 -- X-MS-Has-Attach:
10, 22 -- X-MS-TNEF-Correlator:
10, 22 -- Thread-Topic: instrument comparison
10, 22 -- Thread-Index: AcmnfkFq4y97lJdWRLO1QTGj+WqsHg==
10, 22 -- From: "Hsia, Ru-Ching" {RHsia-at-umaryland.edu}
10, 22 -- To: {Microscopy-at-microscopy.com}
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2I41gGa011069
==============================End of - Headers==============================




From: emlabservices-at-cox.net
Date: Wed, 18 Mar 2009 06:52:22 -0500
Subject: [Microscopy] Wanted: Parts for Jeol 1200 EX (MK1/2)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a limited supply of parts available for this model instrument, as
well as other models by this OEM.

Inquire by email address roberts-at-emlabservices.com.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



==============================Original Headers==============================
5, 33 -- From emlabservices-at-cox.net Wed Mar 18 06:52:22 2009
5, 33 -- Received: from eastrmmtao101.cox.net (eastrmmtao101.cox.net [68.230.240.7])
5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IBqMgd009104
5, 33 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 06:52:22 -0500
5, 33 -- Received: from eastrmimpo03.cox.net ([68.1.16.126])
5, 33 -- by eastrmmtao101.cox.net
5, 33 -- (InterMail vM.7.08.02.01 201-2186-121-102-20070209) with ESMTP
5, 33 -- id {20090318115221.BFEZ15713.eastrmmtao101.cox.net-at-eastrmimpo03.cox.net}
5, 33 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 07:52:21 -0400
5, 33 -- Received: from EMLabServices ([72.205.231.228])
5, 33 -- by eastrmimpo03.cox.net with bizsmtp
5, 33 -- id UbsM1b0084wKcqG02bsMnj; Wed, 18 Mar 2009 07:52:21 -0400
5, 33 -- X-Authority-Analysis: v=1.0 c=1 a=stvMZ3rvt9AA:10 a=PqL8Lxlrfu4A:10
5, 33 -- a=7lK6D5y0AAAA:8 a=vjwulHji36Ww7BFDtVsA:9 a=izG0QIycz_BKrbrKs4t07i66NdAA:4
5, 33 -- a=gi0PWCVxevcA:10 a=5FnZdabJD9cA:10 a=Ukfy9IDRlMMA:10 a=6epph-Xx0CwA:10
5, 33 -- a=nQ4JryvH0u8A:10 a=QdrHNQEAe2YA:10 a=TG08ivR9hdwA:10 a=1e3GG_-JlcFj8G6C:21
5, 33 -- a=VPHQaiF0fLfcu3d2:21
5, 33 -- X-CM-Score: 0.00
5, 33 -- Message-ID: {49EA8F75274B422B8B19D5F814737CF9-at-EMLabServices}
5, 33 -- From: "EM Lab Services" {emlabservices-at-cox.net}
5, 33 -- To: {Microscopy-at-microscopy.com}
5, 33 -- Subject: Wanted: Parts for Jeol 1200 EX (MK1/2)
5, 33 -- Date: Wed, 18 Mar 2009 05:52:27 -0600
5, 33 -- MIME-Version: 1.0
5, 33 -- Content-Type: text/plain;
5, 33 -- format=flowed;
5, 33 -- charset="iso-8859-1";
5, 33 -- reply-type=original
5, 33 -- Content-Transfer-Encoding: 7bit
5, 33 -- X-Priority: 3
5, 33 -- X-MSMail-Priority: Normal
5, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
5, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: dsoren-at-umich.edu
Date: Wed, 18 Mar 2009 07:11:13 -0500
Subject: [Microscopy] What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I am writing to enlist your help in identifying the organism in these
TEM images, which
you can view by going to this link.

http://www.med.umich.edu/cdb/mil/docs/temimages.html

It was isolated from macrophages from the peripheral blood of
sarcoidosis patients. The same organism was also isolated from
the macrophages of a pleural effusion from an HIV positve patient
with diffuse large B cell lymphoma after many months of culture. It
was fixed in glutaraldehyde in Sorensens buffer,pelleted, and
embedded in Histogel. Then the Histogel-embedded pellet was
prepared for TEM by standard techniques, i.e., post fixed with osmium,
en bloc stained with uranyl acetate, dehydrated in a graded series of
ethanol followed by propylene oxide, and infiltrated and embedded in
Epon. Ultra-thin sections were post stained with uranyl acetate and lead
citrate.

Has anyone seen anything like this? We would like to give it its
proper name instead of its current name, The Critter. Any help you
might give us would be appreciated.

Thanks,

Dotty Sorenson


Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
A807 BSRB
109 Zina Pitcher Place
Ann Arbor, MI 48109-2200
(734)763-1170
FAX (734)763-1166



==============================Original Headers==============================
11, 16 -- From dsoren-at-umich.edu Wed Mar 18 07:11:12 2009
11, 16 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81])
11, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2ICBCsP023154
11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 07:11:12 -0500
11, 16 -- Received: FROM [10.21.131.86] (host-18.subnet-17.med.umich.edu [141.214.17.18])
11, 16 -- BY hackers.mr.itd.umich.edu ID 49C0E4A4.5EC54.30427 ;
11, 16 -- 18 Mar 2009 08:10:12 -0400
11, 16 -- Mime-Version: 1.0 (Apple Message framework v753.1)
11, 16 -- Content-Transfer-Encoding: 7bit
11, 16 -- Message-Id: {FD3BC54A-F5AC-43D2-BA3C-36B68D2378A8-at-umich.edu}
11, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
11, 16 -- To: microscopy-at-microscopy.com
11, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
11, 16 -- Subject: What is this critter?
11, 16 -- Date: Wed, 18 Mar 2009 08:10:05 -0400
11, 16 -- X-Mailer: Apple Mail (2.753.1)
==============================End of - Headers==============================




From: giulio.lamedica-at-libero.it
Date: Wed, 18 Mar 2009 08:43:14 -0500
Subject: [Microscopy] viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both giulio.lamedica-at-libero.it as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: giulio.lamedica-at-libero.it
Name: Giulio

Organization: Univ of Rome La Sapienza

Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension

Question: I'm interested in analysing TiO2 50 100nm nanoparticles
in a liquid suspension with FE-SEM.
I'm intereted in particles characterization, because we know exactly
their shape.
What I'd like to investigate is how they are aggregate.
Of course if we dry them we change their aggregation state. Have you
any ideas to suggest?

Thanks

Giulio



Login Host: 82.191.37.58
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Mar 18 08:43:14 2009
10, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IDh8P7007648
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 08:43:11 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c5e6a9345c1a-at-[206.69.208.22]}
10, 11 -- Date: Wed, 18 Mar 2009 08:43:06 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: giulio.lamedica-at-libero.it (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Wed, 18 Mar 2009 09:43:18 -0500
Subject: [Microscopy] PFM4 - Aveiro

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues

We remind you that the 4th *Piezoresponse Force Microscopy* (PFM)
Workshop and the first symposium on *Nanoscale Phenomena in Polar
Materials* (PFM 2009) will take place in Aveiro (Portugal) 23-27 of June
2009.

If you intend to participate, we kindly remind you that the deadline for
registration is approaching rapidly.

We offer a number of tutorial lectures by PFM specialists (see list with
abstracts at http://pfm4.web.ua.pt, hands-on classes (optional) with
advanced setups from *Agilent*, *Asylum*, *NT-MDT* and *Veeco* and
participation in the symposium with the possibility to discuss your
latest results in a warm and cordial atmosphere.

Many renowned scientists have already agreed to participate as plenary,
invited, and tutorial speakers, including M. Alexe, R. Garcia, A.
Gruverman, S. V. Kalinin, W. Kleemann, A. Ruediger, P. Paruch, N.
Pertsev, G. Schneider, J. F. Scott, J.-M. Triscone, and others. Do not
miss this opportunity to learn the latest developments in the rapidly
growing world of *PFM* and *NanoFerroelectrics*!

With our warmest regards, we look forward to seeing you in June in Aveiro.

Sincerely


Sergei V. Kalinin

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
13, 24 -- From sergei2-at-ornl.gov Wed Mar 18 09:43:17 2009
13, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119])
13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IEhGSS023023
13, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:43:17 -0500
13, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1])
13, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
13, 24 -- with ESMTP id {0KGP00IEZI823T-at-emroute1.ornl.gov} for
13, 24 -- microscopy-at-microscopy.com; Wed, 18 Mar 2009 10:43:14 -0400 (EDT)
13, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov
13, 24 -- (PMDF V6.4 #31561) id {0KGP00001I82KI-at-emroute1.ornl.gov} ; Wed,
13, 24 -- 18 Mar 2009 10:43:14 -0400 (EDT)
13, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
13, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
13, 24 -- with ESMTP id {0KGP00JEFI8242-at-emroute1.ornl.gov} ; Wed,
13, 24 -- 18 Mar 2009 10:43:14 -0400 (EDT)
13, 24 -- Date: Wed, 18 Mar 2009 10:43:14 -0400
13, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
13, 24 -- Subject: PFM4 - Aveiro
13, 24 -- To: microscopy-at-microscopy.com, Andrei Kholkin {kholkin-at-ua.pt}
13, 24 -- Message-id: {49C10882.60501-at-ornl.gov}
13, 24 -- MIME-version: 1.0
13, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
13, 24 -- Content-transfer-encoding: 7bit
13, 24 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
==============================End of - Headers==============================




From: paulrc-at-bilbo.bio.purdue.edu
Date: Wed, 18 Mar 2009 10:27:42 -0500
Subject: [Microscopy] Re: What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It looks like a large icosahedral virus and is similar in size to
Herpesvirus. There are not many large (} 100nm) icosahedral viruses that
infect vertebrates but I'd still suggest you consider it. The third
image has one very clear particle in the cytoplasm and near the top of
the same image, a number of particles that look to be budding.
-paul


--
Paul Chipman
Director, Biological Electron Microscopy Facility
Purdue University
765-494-1487


Quoting dsoren-at-umich.edu:

--------------------------------------------------------------
}
} Dear Listers,
}
} I am writing to enlist your help in identifying the organism in these
} TEM images, which
} you can view by going to this link.
}
} http://www.med.umich.edu/cdb/mil/docs/temimages.html
}
} It was isolated from macrophages from the peripheral blood of
} sarcoidosis patients. The same organism was also isolated from
} the macrophages of a pleural effusion from an HIV positve patient
} with diffuse large B cell lymphoma after many months of culture. It
} was fixed in glutaraldehyde in Sorensens buffer,pelleted, and
} embedded in Histogel. Then the Histogel-embedded pellet was
} prepared for TEM by standard techniques, i.e., post fixed with osmium,
} en bloc stained with uranyl acetate, dehydrated in a graded series of
} ethanol followed by propylene oxide, and infiltrated and embedded in
} Epon. Ultra-thin sections were post stained with uranyl acetate and lead
} citrate.
}
} Has anyone seen anything like this? We would like to give it its
} proper name instead of its current name, The Critter. Any help you
} might give us would be appreciated.
}
} Thanks,
}
} Dotty Sorenson
}
}
} Dorothy Sorenson
} Microscopy and Image-analysis Laboratory
} Department of Cell and Developmental Biology
} University Of Michigan Medical School
} A807 BSRB
} 109 Zina Pitcher Place
} Ann Arbor, MI 48109-2200
} (734)763-1170
} FAX (734)763-1166
}


==============================Original Headers==============================
7, 20 -- From paulrc-at-bilbo.bio.purdue.edu Wed Mar 18 10:27:42 2009
7, 20 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IFRe2O005496
7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:27:40 -0500
7, 20 -- Received: from dhcp18-69-fixed.bio.purdue.edu (dhcp18-69-fixed.bio.purdue.edu [128.210.18.69])
7, 20 -- (authenticated bits=0)
7, 20 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-auth.purdue.edu) with ESMTP id n2IFRcIC000907
7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:27:38 -0400
7, 20 -- Message-ID: {49C112EA.6060703-at-bilbo.bio.purdue.edu}
7, 20 -- Date: Wed, 18 Mar 2009 11:27:38 -0400
7, 20 -- From: "Chipman, Paul R" {paulrc-at-bilbo.bio.purdue.edu}
7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421)
7, 20 -- MIME-Version: 1.0
7, 20 -- To: microscopy-at-microscopy.com
7, 20 -- Subject: Re: [Microscopy] What is this critter?
7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- X-PMX-Version: 5.4.0.320885
7, 20 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: lgordon-at-gmail.com
Date: Wed, 18 Mar 2009 11:25:34 -0500
Subject: [Microscopy] Re: viaWWW: TiO2 50 100nm nanoparticles in a liquid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Giulio,

If you don't want to dry the particles you could deposit a small
volume onto a TEM grid and plunge freeze the grid in liquid ethane at
liq. N2 temps and looks at it in a cryo-TEM. You didn't mention what
medium the nanoparticles were dispersed in but if its water that
should work. Some sort of x-ray or light (laser) scattering technique
might be able to identify the aggregate size without needing to dry
the suspension.

AFM in solution could work but I don't know how easy it would be.
There could be an easier way to get a TEM sample.

That's all I can think of right now.

Good luck.

-Lyle

--
Lyle Gordon
Department of Materials Science and Engineering
Northwestern University

2220 Campus Drive
Evanston, IL 60208

Tel: (847) 491-3584
Mobile: (8470) 400-4071
lgordon-at-u.northwestern.edu



On Wed, Mar 18, 2009 at 8:57 AM, {giulio.lamedica-at-libero.it} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please  copy  both giulio.lamedica-at-libero.it as well as   the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: giulio.lamedica-at-libero.it
} Name: Giulio
}
} Organization: Univ of Rome La Sapienza
}
} Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension
}
} Question: I'm interested in analysing TiO2 50 100nm nanoparticles
} in a liquid suspension with FE-SEM.
} I'm intereted in particles characterization, because we know exactly
} their shape.
} What I'd like to investigate is how they are aggregate.
} Of course if we dry them we change their aggregation state. Have you
} any ideas to suggest?
}
} Thanks
}
} Giulio
}
}
}
}  Login Host: 82.191.37.58
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Wed Mar 18 08:43:14 2009
} 10, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
} 10, 11 --       by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IDh8P7007648
} 10, 11 --       for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 08:43:11 -0500
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240800c5e6a9345c1a-at-[206.69.208.22]}
} 10, 11 -- Date: Wed, 18 Mar 2009 08:43:06 -0500
} 10, 11 -- To: microscopy-at-microscopy.com
} 10, 11 -- From: giulio.lamedica-at-libero.it (by way of MicroscopyListserver)
} 10, 11 -- Subject: viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension
} 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
13, 36 -- From lgordon-at-gmail.com Wed Mar 18 11:25:34 2009
13, 36 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.248])
13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGPWJY021029
13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:25:33 -0500
13, 36 -- Received: by an-out-0708.google.com with SMTP id b38so89938ana.0
13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:25:29 -0700 (PDT)
13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 36 -- d=gmail.com; s=gamma;
13, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references
13, 36 -- :date:message-id:subject:from:to:content-type
13, 36 -- :content-transfer-encoding;
13, 36 -- bh=fIaxaDc+wIHyVP9DlOlM6Xl+P/H7eKMGpGJIWms6hLA=;
13, 36 -- b=T2jEQJlFahKiv+1MpxYne1tzXg726XvR/SWSrzLLTU0TWe+7iBRsIIwq90Emss0IU1
13, 36 -- q3NYYr4x0Fv1lswjATBW8/sZYcsgSQg4N3QcepNHj6RKmzKzJR0lAH0zkZw1v4nG7/11
13, 36 -- A/VykP3o967ysCrDxxhNIPdseZhN3DpeksTng=
13, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
13, 36 -- d=gmail.com; s=gamma;
13, 36 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
13, 36 -- :content-type:content-transfer-encoding;
13, 36 -- b=De9hStseVzHaMaVdOaUeAgWWJd1lRTk2mmVZ68wO2aLrR1LUNGGqcb5U5eInJ5Ja8Z
13, 36 -- BqEDXp9pcs/xuxXhafCI8Vhz0qL7XDlJK8OMSbQchwnWTK9erIFCqs9Uw7k7taz03uLx
13, 36 -- MgtkhiVKmFcSSaDtKf+hHgserhtplm1cYDsVM=
13, 36 -- MIME-Version: 1.0
13, 36 -- Received: by 10.101.70.14 with SMTP id x14mr1691423ank.131.1237393529097; Wed,
13, 36 -- 18 Mar 2009 09:25:29 -0700 (PDT)
13, 36 -- In-Reply-To: {200903181357.n2IDveBx021692-at-ns.microscopy.com}
13, 36 -- References: {200903181357.n2IDveBx021692-at-ns.microscopy.com}
13, 36 -- Date: Wed, 18 Mar 2009 11:25:29 -0500
13, 36 -- Message-ID: {88f183960903180925k551063a1lc5996951a116320c-at-mail.gmail.com}
13, 36 -- Subject: Re: [Microscopy] viaWWW: TiO2 50 100nm nanoparticles in a liquid
13, 36 -- suspension
13, 36 -- From: Lyle Gordon {lgordon-at-gmail.com}
13, 36 -- To: giulio.lamedica-at-libero.it, microscopy-at-microscopy.com
13, 36 -- Content-Type: text/plain; charset=ISO-8859-1
13, 36 -- Content-Transfer-Encoding: 8bit
13, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2IGPWJY021029
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Wed, 18 Mar 2009 11:44:11 -0500
Subject: [Microscopy] What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dotty, et al

Actually, the answer is in the original description of the specimens
"from an HIV positve patient". Unfortunately the preparation in
Histogel has caused some deterioration in quality of specimen at the end
point. What you are seeing is lentivirus particles. given the specimen
source you can assume that they are the human version - ie HIV particles.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Mar 18 11:44:10 2009
6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGi8D4002743
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:44:10 -0500
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 -- (authenticated bits=0)
6, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2IGi5lZ013388;
6, 20 -- Wed, 18 Mar 2009 11:44:05 -0500 (CDT)
6, 20 -- Message-ID: {49C124D4.8050302-at-umanitoba.ca}
6, 20 -- Date: Wed, 18 Mar 2009 11:44:04 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: dsoren-at-umich.edu
6, 20 -- Subject: Re: [Microscopy] Re: What is this critter?
6, 20 -- References: {200903181530.n2IFUrHM008486-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200903181530.n2IFUrHM008486-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: glenmac-at-u.washington.edu
Date: Wed, 18 Mar 2009 12:03:06 -0500
Subject: [Microscopy] IR filters for DIC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
What wavelengths are labs using for IR filters used with IR-DIC such
as with patch clamping? We have a microscope on an old, heavily used,
patch clamp rig with a badly degraded IR filter. Dodt and
Zeiglgansberger, 1997 cite a Schott filter with 780 nm maximum, Omega
sells a 780/40, Chroma offers a Schott glass filter } 780. This
microscope has no documentation for the filter and none of the vendors
I've contacted recognized the part number on the filter.

Thanks,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************



==============================Original Headers==============================
7, 24 -- From glenmac-at-u.washington.edu Wed Mar 18 12:03:05 2009
7, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IH351X016969
7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 12:03:05 -0500
7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.141] (may be forged))
7, 24 -- by mxout1.cac.washington.edu (8.14.3+UW09.02/8.14.3+UW09.01) with ESMTP id n2IH342S028861
7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK)
7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:03:04 -0700
7, 24 -- X-Auth-Received: from D-128-95-178-135.dhcp4.washington.edu (D-128-95-178-135.dhcp4.washington.edu [128.95.178.135])
7, 24 -- (authenticated authid=glenmac)
7, 24 -- by smtp.washington.edu (8.14.3+UW09.02/8.14.3+UW09.01) with ESMTP id n2IH34Hq021937
7, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT)
7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:03:04 -0700
7, 24 -- Message-Id: {D8E8FA16-97C5-44A5-BC56-6BD6D8949849-at-u.washington.edu}
7, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu}
7, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com}
7, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 24 -- Content-Transfer-Encoding: 7bit
7, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3)
7, 24 -- Subject: IR filters for DIC
7, 24 -- Date: Wed, 18 Mar 2009 10:03:04 -0700
7, 24 -- X-Mailer: Apple Mail (2.930.3)
7, 24 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.18.164927
7, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=8%, Report='REPLICA_BRANDS 0.05, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_900_999 0, TO_NO_NAME 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0'
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Wed, 18 Mar 2009 12:08:35 -0500
Subject: [Microscopy] What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with Paul as to it being a virus... If not herpes than possibly a
paramyxovirus virus such as measles that also is an enveloped virus that
buds from the cytoplasmic membrane. I am curious as the the spikey stuff all
around. Was a phosphate buffer used at some point prior to UA en-bloc
staining? This can cause formation of phosphate crystals that look very
much like what you have. If this was not present than you could probably
more clearly see the formation of the virus as it buds from the cytoplasmic
membrane.
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: Paul Chipman {paulrc-at-bilbo.bio.purdue.edu}
} Reply-To: Paul Chipman {paulrc-at-bilbo.bio.purdue.edu}
} Date: Wed, 18 Mar 2009 10:31:10 -0500
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] Re: What is this critter?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} It looks like a large icosahedral virus and is similar in size to
} Herpesvirus. There are not many large (} 100nm) icosahedral viruses that
} infect vertebrates but I'd still suggest you consider it. The third
} image has one very clear particle in the cytoplasm and near the top of
} the same image, a number of particles that look to be budding.
} -paul
}
}
} --
} Paul Chipman
} Director, Biological Electron Microscopy Facility
} Purdue University
} 765-494-1487
}
}
} Quoting dsoren-at-umich.edu:
}
} --------------------------------------------------------------
} }
} } Dear Listers,
} }
} } I am writing to enlist your help in identifying the organism in these
} } TEM images, which
} } you can view by going to this link.
} }
} } http://www.med.umich.edu/cdb/mil/docs/temimages.html
} }
} } It was isolated from macrophages from the peripheral blood of
} } sarcoidosis patients. The same organism was also isolated from
} } the macrophages of a pleural effusion from an HIV positve patient
} } with diffuse large B cell lymphoma after many months of culture. It
} } was fixed in glutaraldehyde in Sorensens buffer,pelleted, and
} } embedded in Histogel. Then the Histogel-embedded pellet was
} } prepared for TEM by standard techniques, i.e., post fixed with osmium,
} } en bloc stained with uranyl acetate, dehydrated in a graded series of
} } ethanol followed by propylene oxide, and infiltrated and embedded in
} } Epon. Ultra-thin sections were post stained with uranyl acetate and lead
} } citrate.
} }
} } Has anyone seen anything like this? We would like to give it its
} } proper name instead of its current name, The Critter. Any help you
} } might give us would be appreciated.
} }
} } Thanks,
} }
} } Dotty Sorenson
} }
} }
} } Dorothy Sorenson
} } Microscopy and Image-analysis Laboratory
} } Department of Cell and Developmental Biology
} } University Of Michigan Medical School
} } A807 BSRB
} } 109 Zina Pitcher Place
} } Ann Arbor, MI 48109-2200
} } (734)763-1170
} } FAX (734)763-1166
} }
}
}
} ==============================Original Headers==============================
} 7, 20 -- From paulrc-at-bilbo.bio.purdue.edu Wed Mar 18 10:27:42 2009
} 7, 20 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu
} [128.210.5.131])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2IFRe2O005496
} 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:27:40 -0500
} 7, 20 -- Received: from dhcp18-69-fixed.bio.purdue.edu
} (dhcp18-69-fixed.bio.purdue.edu [128.210.18.69])
} 7, 20 -- (authenticated bits=0)
} 7, 20 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-auth.purdue.edu)
} with ESMTP id n2IFRcIC000907
} 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
} 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:27:38 -0400
} 7, 20 -- Message-ID: {49C112EA.6060703-at-bilbo.bio.purdue.edu}
} 7, 20 -- Date: Wed, 18 Mar 2009 11:27:38 -0400
} 7, 20 -- From: "Chipman, Paul R" {paulrc-at-bilbo.bio.purdue.edu}
} 7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421)
} 7, 20 -- MIME-Version: 1.0
} 7, 20 -- To: microscopy-at-microscopy.com
} 7, 20 -- Subject: Re: [Microscopy] What is this critter?
} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 20 -- Content-Transfer-Encoding: 7bit
} 7, 20 -- X-PMX-Version: 5.4.0.320885
} 7, 20 -- X-PerlMx-Virus-Scanned: Yes
} ==============================End of - Headers==============================


==============================Original Headers==============================
4, 30 -- From dsherman-at-purdue.edu Wed Mar 18 12:08:35 2009
4, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129])
4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IH8Ytn028628
4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 12:08:34 -0500
4, 30 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127])
4, 30 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n2IH8YfL003579
4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 13:08:34 -0400
4, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8])
4, 30 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n2IH8YgN005066
4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 13:08:34 -0400
4, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959);
4, 30 -- Wed, 18 Mar 2009 13:08:33 -0400
4, 30 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ;
4, 30 -- Wed, 18 Mar 2009 17:07:45 +0000
4, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119
4, 30 -- Date: Wed, 18 Mar 2009 13:07:43 -0500
4, 30 -- Subject: Re: [Microscopy] Re: What is this critter?
4, 30 -- From: Debby Sherman {dsherman-at-purdue.edu}
4, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
4, 30 -- Message-ID: {C5E6A29F.3BDA4%dsherman-at-exchange.purdue.edu}
4, 30 -- Thread-Topic: [Microscopy] Re: What is this critter?
4, 30 -- Thread-Index: Acmn7A0PiVsm07dpQ2K0VEUXHU2gOA==
4, 30 -- In-Reply-To: {200903181531.n2IFVALC008783-at-ns.microscopy.com}
4, 30 -- Mime-version: 1.0
4, 30 -- Content-type: text/plain;
4, 30 -- charset="US-ASCII"
4, 30 -- Content-transfer-encoding: 7bit
4, 30 -- X-OriginalArrivalTime: 18 Mar 2009 17:08:33.0980 (UTC) FILETIME=[2B7247C0:01C9A7EC]
4, 30 -- X-PMX-Version: 5.4.0.320885
4, 30 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 18 Mar 2009 13:42:43 -0500
Subject: [Microscopy] CL for JXA-840A?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Does anyone know if it is or isn't possible to fit CL to a JEOL 840 which already has 3 x WDS
and 1 x EDS as well as the standard 840 Optical Microscope?

There's not a lot of room left in there, but maybe something involving fibre optics or the OM
might fit in.

What if there was only the EDS and the OM ie no WDS?

Vendors' replies welcome.

cheers

Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
9, 28 -- From r.sims-at-auckland.ac.nz Wed Mar 18 13:42:43 2009
9, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IIgcup014811
9, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 13:42:42 -0500
9, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 686EA9DC35
9, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 07:42:37 +1300 (NZDT)
9, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
9, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
9, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
9, 28 -- with ESMTP id OCbPxNLcvdQc for {microscopy-at-Microscopy.Com} ;
9, 28 -- Thu, 19 Mar 2009 07:42:37 +1300 (NZDT)
9, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
9, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
9, 28 -- (No client certificate requested)
9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 48D739DB52
9, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 07:42:37 +1300 (NZDT)
9, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
9, 28 -- To: microscopy-at-Microscopy.Com
9, 28 -- Date: Thu, 19 Mar 2009 07:44:51 +1300
9, 28 -- MIME-Version: 1.0
9, 28 -- Subject: CL for JXA-840A?
9, 28 -- Message-ID: {49C1F7F3.14890.AC737-at-r.sims.auckland.ac.nz}
9, 28 -- Priority: normal
9, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
9, 28 -- Content-type: text/plain; charset=US-ASCII
9, 28 -- Content-transfer-encoding: 7BIT
9, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: vishnu.mogili-at-gmail.com
Date: Wed, 18 Mar 2009 19:11:25 -0500
Subject: [Microscopy] Thickness of sample by CBED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

     Recently I have started to work on Convergent Beam Electron
Diffraction. So I have very basic questions. Please help me in gaining
knowledge in the related field.

My first goal is to determine the thickness of MAGICAL sample using
CBED. Here are my problems.

1. When I was taking a CBED pattern at lower symmetry, I was unable to
find any K-M fringes on central spot {000} . I have varied camera
length from 30cm to 300cm and also  changed exposure time from 0.1 sec
to 30 sec. I was neither able to find any fringes on the central spot
nor was able to find spot next to it.

    Basically to find thickness, we mainly require 2 spots next to
each other. One is central spot and other one is {220} or {200} of
CBED pattern. I was unable to get these 2 spots next to each other.
Please tell me what are the variables need to be changed in JEOL 2011
TEM so that we get a pattern where spots are nearby to each other.

2.  According to Williams and Carter text book, to get KM fringes,
angle of convergence (2α) should be less than Bragg’s angle (2θ). Can
you specify how can we change angle of convergence and Bragg’s angle
in JEOL 2011??? If I’m correct, angle of convergence is alpha selector
and Bragg’s angle is magnification toggle of JEOL 2011??? If I’m
wrong, please correct me.

3. Right now I’m using accelerating voltage of 200kv. Does it have any
impact on CBED patterns once if I change it to 100kv or 150kv???


Thanks for your time

Vishnu Mogili,

PhD student,
Materials & Surface Science Institute,
University of Limerick,
Ireland.


==============================Original Headers==============================
12, 32 -- From vishnu.mogili-at-gmail.com Wed Mar 18 19:11:24 2009
12, 32 -- Received: from mail-bw0-f159.google.com (mail-bw0-f159.google.com [209.85.218.159])
12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J0BNJ5006613
12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 19:11:23 -0500
12, 32 -- Received: by bwz3 with SMTP id 3so300315bwz.18
12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 17:11:22 -0700 (PDT)
12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
12, 32 -- :from:to:content-type:content-transfer-encoding;
12, 32 -- bh=kT5jj0QwnaSNbzDccQ8byDANlS2FiPyTuPyQK8yeyv0=;
12, 32 -- b=WPiZ6hAcKtGOTjWqZZtfJbxJ5iG82nZ9fCeiRgN69c/aOaNknHi008k543GSFLSDm2
12, 32 -- Ib53RNxyjcvGMmV09khhSvviBQ+6GZgWEMmGcgsp/FxqNZ0zSn5U9jOWLgODvp672PQP
12, 32 -- Xd5MYnIOrHI72IvA7J7jRMOEQByBJQoLUxLYM=
12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
12, 32 -- d=gmail.com; s=gamma;
12, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
12, 32 -- :content-transfer-encoding;
12, 32 -- b=TnJ9ILBn1umrl5E2km9IjvP8Cod2qvqTLf78Tq3Ju7N3Xb8in5zJa+IQIZhdJkc0s1
12, 32 -- 40lwuVsbzNO8jEDXJASuGrAQhq1KG/HkDdaFM8tUc5LBbDuwCeYRByT84iLz0BThhy/f
12, 32 -- 5T6mCYsdBHaBEwfrc5kcv9Uq9LidM1BZFmKK0=
12, 32 -- MIME-Version: 1.0
12, 32 -- Received: by 10.223.113.199 with SMTP id b7mr1549916faq.82.1237421482494; Wed,
12, 32 -- 18 Mar 2009 17:11:22 -0700 (PDT)
12, 32 -- Date: Thu, 19 Mar 2009 00:11:22 +0000
12, 32 -- Message-ID: {95c1e3310903181711n33f82d6dr94cee590b1289de-at-mail.gmail.com}
12, 32 -- Subject: Thickness of sample by CBED
12, 32 -- From: vishnu mogili {vishnu.mogili-at-gmail.com}
12, 32 -- To: Microscopy-at-microscopy.com
12, 32 -- Content-Type: text/plain; charset=UTF-8
12, 32 -- Content-Transfer-Encoding: 8bit
12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2J0BNJ5006613
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 18 Mar 2009 20:28:02 -0500
Subject: [Microscopy] Benchtop CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again

What's the cheapest way of getting into CL of quartz? Can CL be put onto a benchtop SEM?

cheers

Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
6, 28 -- From r.sims-at-auckland.ac.nz Wed Mar 18 20:28:01 2009
6, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J1RvM5030297
6, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 20:28:01 -0500
6, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1BE859DFD1
6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:56 +1300 (NZDT)
6, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
6, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
6, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
6, 28 -- with ESMTP id IwqwR4nhAL-X for {microscopy-at-Microscopy.Com} ;
6, 28 -- Thu, 19 Mar 2009 14:27:56 +1300 (NZDT)
6, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
6, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
6, 28 -- (No client certificate requested)
6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id EC4C59DFCB
6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:55 +1300 (NZDT)
6, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
6, 28 -- To: microscopy-at-Microscopy.Com
6, 28 -- Date: Thu, 19 Mar 2009 14:30:10 +1300
6, 28 -- MIME-Version: 1.0
6, 28 -- Subject: Benchtop CL
6, 28 -- Message-ID: {49C256F2.21397.17DDAB3-at-r.sims.auckland.ac.nz}
6, 28 -- Priority: normal
6, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
6, 28 -- Content-type: text/plain; charset=US-ASCII
6, 28 -- Content-transfer-encoding: 7BIT
6, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: hartfield-at-omniprobe.com
Date: Wed, 18 Mar 2009 20:55:18 -0500
Subject: [Microscopy] viaWWW: AMFA 2009, April 2-3, Santa Clara

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hartfield-at-omniprobe.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hartfield-at-omniprobe.com
Name: Cheryl Hartfield

Organization: Omniprobe

Title-Subject: [Filtered] Don't Miss this Opportunity -- AMFA 2009,
April 2-3, Santa Clara

Question: Dear colleagues,

In just two weeks, an impressive line-up of speakers will discuss
"enabling and disruptive technologies" at the multidisciplinary
Advanced Materials/ Failure Analysis (AMFA) Workshop -
www.amfaworkshop.org.

This is a one-day workshop following a day of educational courses and
a 4 hour workshop on government funding for failure analysis. The
registration fee is nominal, and the 2009 program continues the AMFA
tradition of new information presented by leading-edge researchers.
This is an opportunity you won't want to miss. The workshop on
government funding for FA is free, but invitation only - please
contact me if you wish an invitation.
--------------------------------------------------------------------------------------------------------------

The 2009 AMFA Workshop is financially sponsored by Omniprobe, Inc.,
FEI Company, and ARC Technologies. Technical co-sponsors include the
IEEE Reliability Society and the Electronic Device Failure Analysis
Society. Members of the societies receive a registration discount.

PROGRAM
This year's exciting program consists of renowned invited speakers
covering a variety of enabling and disruptive topics in a series of
40 minute presentations, each followed by 20 minutes of
facilitator-led audience discussion.

Ed Principe - Zeiss, Inc.
Nanotomography and Applications of Stereolithography

Joseph Michael - Sandia National Laboratories
What's New in Electrons, Ions and X-rays

Dieter Schroder - Arizona State University
Characterization of Nano Devices and Nano Characterization of Materials

Kevin Hemker - John Hopkins University
Lilliputian techniques for characterizing mechanical properties

William Vanderlinde - Laboratory for Physical Sciences Government
funding for FA tool development

Jerry Mearini -Teraphysics Corporation
Present and Near-Future Detection and Identification Capabilities of
Terahertz Technologies

Kevin Bennett - Arizona State University
Structural and molecular magnetic resonance microscopy: a biological
perspective

On site registration will be available. Please visit
www.amfaworkshop.org for details. We look forward to seeing you.

Best Regards,
Cheryl Hartfield
AMFA 2009 Committee Member



Login Host: 216.74.250.210
---------------------------------------------------------------------------


==============================Original Headers==============================
22, 21 -- From zaluzec-at-microscopy.com Wed Mar 18 20:55:18 2009
22, 21 -- Received: from s-utl02-dcpop.stsn.net (s-utl02-dcpop.stsn.net [72.255.0.202])
22, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J1tHUE012037
22, 21 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 20:55:18 -0500
22, 21 -- Received: from s-utl02-dcpop.stsn.net ([127.0.0.1])
22, 21 -- by s-utl02-dcpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2009031821551518531
22, 21 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 21:55:15 -0400
22, 21 -- X-Spam-Status: No, hits=0.0 required=9.9
22, 21 -- tests=ALL_TRUSTED: -2.867,BAYES_00: -1.665
22, 21 -- X-Spam-Level:
22, 21 -- Received: from [10.0.239.114] ([10.0.239.114])
22, 21 -- by s-utl02-dcpop.stsn.net
22, 21 -- for microscopy-at-microscopy.com;
22, 21 -- Wed, 18 Mar 2009 21:55:13 -0400
22, 21 -- Mime-Version: 1.0
22, 21 -- Message-Id: {p06240801c5e7565c20d7-at-[10.0.239.114]}
22, 21 -- Date: Wed, 18 Mar 2009 20:55:02 -0500
22, 21 -- To: microscopy-at-microscopy.com
22, 21 -- From: hartfield-at-omniprobe.com (by way of MicroscopyListserver)
22, 21 -- Subject: viaWWW: AMFA 2009, April 2-3, Santa Clara
22, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Wed, 18 Mar 2009 22:15:18 -0500
Subject: [Microscopy] Re: TEM - Thickness of Sample by CBED.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Vishnu

1. If you are unable to see K-M fringes in your CBED disks, it may be that
your specimen is too thin. The number of fringes increases with thickness
and where your thickness is less than about half an extinction distance (for
the reflection you are using Si(220) E=96nm at 200kV - so {50nm and you may
get nothing. Similarly if your specimen is exceedingly thick and you aren't
using an energy filter, you might find the fringes are wiped out. Also if
you have a large variation in thickness in the region probed, the fringes
will blur into each other. Choose a very flat region of specimen.

Check out J. Microsc 224 (2006) 187-196, where I describe some thickness by
CBED experiments with silicon and P91 on a JEOL 2010. This work describes
use of Vincent Hou's excellent DigitalMicrograph script for carrying out the
thickness calculation. If you are capturing images using DigitalMicrograph,
you can install this script - it makes the calculation a breeze - get it
(Thickness by CBED) from the DigitalMicrograph Script Database (URL at the
bottom of this message).

Thickness determination is achieved by setting up two beam conditions - the
(000) transmitted spot and another diffracted beam are intense - not two
diffracted beams as your post suggests (to me). The choice of which beam to
use isn't too important, but since the extinction distance varies with the
reflection, then the minimum thickness you can measure is determined by your
choice of diffracted beam (for the reasons mentioned in 1). It's best to use
low index reflections - high index reflection have longer extinction
distances. You only need to measure the fringe spacing within the diffracted
beam for the thickness calculation. However, in order to convert this
distance measurement into an angle, you need the transmitted beam present,
since the distance from the edge of the transmitted beam to the edge of the
diffracted beam corresponds to the angle 2theta (Bragg equation) which gives
you the distance to angle calibration. To minimise measurement error capture
the patterns at a camera length such that the spots span a large proportion
of the screen.

2. Set up your CBED conditions so that the transmitted (000) and diffracted
beams are large enough to almost touch. The larger they are the smaller the
measurement error will be. However, if they overlap, you may find making the
disk edge to disk edge measurement difficult. Experiment with both the alpha
control on your JEOL and also the condenser aperture to understand how these
affect your CBED pattern.

3. Changing the microscope voltage will change your wavelength, and since
wavelength appears in the Bragg equation (nL=2dsin(theta)), your Bragg angle
(half the disk edge to disk edge) distance will change (your patterns are
bigger at lower voltage for a given camera length). Also, the extinction
distance - which appears in the thickness equation will change. However,
from a practical perspective the measurement you make will be correct at any
voltage, provided you measure the fringe spacing and disk edge to disk edge
distance correctly and you supply the correct extinction distance (ie don't
use the 200kV value if you are working at lower voltage).

Finally the CBED method is accurate but time consuming. If you have an
energy filter, thickness mapping is much easier to do. However, you do need
a good value for the mean free path in order to convert your map into true
thickness (see the earlier reference on how to measure the mean free path).
There is also a DigitalMicrograph script which will help you estimate the
mean free path (Mean Free Path Estimator). It is described in the reference
I gave you, and today I have posted a much improved version of it to DM
Script Database ( http://www.felmi-zfe.tugraz.at/dm_scripts/welcome.html) -
it may take a week or so to appear.

Regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


==============================Original Headers==============================
14, 24 -- From david.mitchell-at-emu.usyd.edu.au Wed Mar 18 22:15:18 2009
14, 24 -- Received: from sanaa.ucc.usyd.edu.au (sanaa.ucc.usyd.edu.au [129.78.64.145])
14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J3FHX9027662
14, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 22:15:17 -0500
14, 24 -- Received: from sanaa.ucc.usyd.edu.au (localhost [127.0.0.1])
14, 24 -- by localhost (Postfix) with SMTP id E4F58177516
14, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 14:15:14 +1100 (EST)
14, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19])
14, 24 -- by sanaa.ucc.usyd.edu.au (Postfix) with ESMTP id D6D31177508
14, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 14:15:14 +1100 (EST)
14, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.22]) with Microsoft Exchange Server HTTP-DAV ;
14, 24 -- Thu, 19 Mar 2009 03:15:14 +0000
14, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618
14, 24 -- Date: Thu, 19 Mar 2009 14:15:13 +1100
14, 24 -- Subject: Re: TEM - Thickness of Sample by CBED.
14, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
14, 24 -- To: {Microscopy-at-microscopy.com}
14, 24 -- Message-ID: {C5E803F1.540%david.mitchell-at-emu.usyd.edu.au}
14, 24 -- Thread-Topic: TEM - Thickness of Sample by CBED.
14, 24 -- Thread-Index: AcmoQOrzKbI+BBQ0Ed6UXQAjMrpsqg==
14, 24 -- Mime-version: 1.0
14, 24 -- Content-type: text/plain;
14, 24 -- charset="US-ASCII"
14, 24 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: petra.wahlbring-at-goodyear.com
Date: Thu, 19 Mar 2009 03:35:47 -0500
Subject: [Microscopy] Re: TEM - Inviation to a new EM community web site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is an invitation to you to a brand new electron microscopy community
web site:
http://www.emfocal.com

This site focuses on information sharing and community. Our intention on
this site is to make it a “wikipedia-like” information repository: a user
collaborated information base about anything related to electron
microscopy, micro-analysis, applications, …

I invite you come to take a look of the site. If you like it, help us to
build it.

Feel free to forward this message.

Thank you,



==============================Original Headers==============================
7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009
7, 31 -- Received: from outbound-mail-357.bluehost.com (outbound-mail-357.bluehost.com [66.147.249.251])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J3VJln009306
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 22:31:19 -0500
7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37 -0000
7, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161)
7, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar 2009 03:26:37 -0000
7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com;
7, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
7, 31 -- b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;
7, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com)
7, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 31 -- (envelope-from {samwarren-at-emfocal.com} )
7, 31 -- id 1Lk8yV-0006fA-BN
7, 31 -- for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19 -0600
7, 31 -- Received: from 124.192.231.32 ([124.192.231.32])
7, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 31 -- by host361.hostmonster.com with HTTP;
7, 31 -- Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Message-ID: {1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com}
7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Subject: TEM - Inviation to a new EM community web site
7, 31 -- From: samwarren-at-emfocal.com
7, 31 -- To: Microscopy-at-Microscopy.Com
7, 31 -- User-Agent: SquirrelMail/1.4.13
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain;charset=iso-8859-1
7, 31 -- Content-Transfer-Encoding: 8bit
7, 31 -- X-Priority: 3 (Normal)
7, 31 -- Importance: Normal
7, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server}
==============================End of - Headers==============================

From antonio.mora.pi-at-terra.com Thu Mar 19 01:19:52 2009
Return-Path: {antonio.mora.pi-at-terra.com}
Received: from google.com (eatkyo586106.adsl.ppp.infoweb.ne.jp [202.233.193.106])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J6Jomb027341
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 19 Mar 2009 01:19:51 -0500
Received: from [187.156.23.93] (HELO google.com)
by foggy-sneak.net; Thu, 19 Mar 2009 15:13:31 +0900

Dear ...,

looking at your below linked site, I fail completely to see who is behind
that. An individual, a company? On your home page you say "I", in the
policy it says "we" and "our". Who?
The content of this page goes against zero at the moment, but the policy
makes clear that "you" will hold the right on everything published unless
otherwise noted by the contributor. You also intend to benefit from
commercial advertizements: "Please contact us if you wish to support this
site through advertisements."
With the anonymity practiced, I have the strong feeling that this site
intends to make profit from the knowledge of our community and our
willingness to share it. I personally think there are better places to
practice this (e.g. this list server).

Petra
__________________________________
Dr. Petra Wahlbring
Lead Engineer Analytical Test Lab
Goodyear S.A. Technical Center
Colmar-Berg, Luxembourg
e-mail: petra.wahlbring-at-goodyear.com
phone: +352 8199 3725 or GTN 631 3725
fax: +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be
copied or disseminated without the express written consent of The Goodyear
Tire & Rubber Company. -



samwarren-at-emfocal
.com
To
03/19/09 04:33 AM petra.wahlbring-at-goodyear.com
cc

Please respond to Subject
samwarren-at-emfocal [Microscopy] TEM - Inviation to a
.com new EM community web site













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This is an invitation to you to a brand new electron microscopy community
web site:
http://www.emfocal.com

This site focuses on information sharing and community. Our intention on
this site is to make it a “wikipedia-like†information repository: a user
collaborated information base about anything related to electron
microscopy, micro-analysis, applications, …

I invite you come to take a look of the site. If you like it, help us to
build it.

Feel free to forward this message.

Thank you,



==============================Original
Headers==============================
7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009
7, 31 -- Received: from outbound-mail-357.bluehost.com
(outbound-mail-357.bluehost.com [66.147.249.251])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n2J3VJln009306
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009
22:31:19 -0500
7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37
-0000
7, 31 -- Received: from unknown (HELO host361.hostmonster.com)
(66.147.240.161)
7, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar
2009 03:26:37 -0000
7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default;
d=emfocal.com;
7, 31 --
h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;

7, 31 -- b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby
+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;

7, 31 -- Received: from localhost ([127.0.0.1]
helo=host361.hostmonster.com)
7, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 31 -- (envelope-from {samwarren-at-emfocal.com} )
7, 31 -- id 1Lk8yV-0006fA-BN
7, 31 -- for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19
-0600
7, 31 -- Received: from 124.192.231.32 ([124.192.231.32])
7, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 31 -- by host361.hostmonster.com with HTTP;
7, 31 -- Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Message-ID:
{1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com}
7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Subject: TEM - Inviation to a new EM community web site
7, 31 -- From: samwarren-at-emfocal.com
7, 31 -- To: Microscopy-at-Microscopy.Com
7, 31 -- User-Agent: SquirrelMail/1.4.13
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain;charset=iso-8859-1
7, 31 -- Content-Transfer-Encoding: 8bit
7, 31 -- X-Priority: 3 (Normal)
7, 31 -- Importance: Normal
7, 31 -- X-Identified-User:
{2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running
on server}
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 17 -- From petra.wahlbring-at-goodyear.com Thu Mar 19 03:35:47 2009
25, 17 -- Received: from emeam1.goodyear.com (emeam1.goodyear.com [57.67.226.7])
25, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J8ZkcU030439
25, 17 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 03:35:47 -0500
25, 17 -- In-Reply-To: {200903190333.n2J3XnG8014706-at-ns.microscopy.com}
25, 17 -- Subject: Re: [Microscopy] TEM - Inviation to a new EM community web site
25, 17 -- To: microscopy-at-microscopy.com
25, 17 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
25, 17 -- Message-ID: {OFBA11D716.1615D217-ONC125757E.002D858E-C125757E.002F2C9E-at-goodyear.com}
25, 17 -- From: petra.wahlbring-at-goodyear.com
25, 17 -- Date: Thu, 19 Mar 2009 09:35:16 +0100
25, 17 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 8.0.1|February 07, 2008) at
25, 17 -- 03/19/2009 09:35:51 AM
25, 17 -- MIME-Version: 1.0
25, 17 -- Content-type: text/plain; charset=UTF-8
25, 17 -- Content-Transfer-Encoding: 8bit
25, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2J8ZkcU030439
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 19 Mar 2009 06:48:50 -0500
Subject: [Microscopy] viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ciao Giulio!

In water I have never seen TiO2 particles aggregate.
Now to answer your question:
- You only need several particles to aggregate in order to see it in light microscopy. Why bother with EM?
- Just filter a suspension of particles! I cannot recommend you enough the anopore filters. They are simply fantastic!

Per altre domande non esitare a scrivermi.

Best regards,

Stephane


 


----- Original Message ----
X-from: "giulio.lamedica-at-libero.it" {giulio.lamedica-at-libero.it}
To: nizets2-at-yahoo.com
Sent: Wednesday, March 18, 2009 2:51:20 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both giulio.lamedica-at-libero.it as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: giulio.lamedica-at-libero.it
Name: Giulio

Organization: Univ of Rome La Sapienza

Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension

Question: I'm interested in analysing TiO2 50 100nm nanoparticles
in a liquid suspension with FE-SEM.
I'm intereted in particles characterization, because we know exactly
their shape.
What I'd like to investigate is how they are aggregate.
Of course if we dry them we change their aggregation state. Have you
any ideas to suggest?

Thanks

Giulio



  Login Host: 82.191.37.58
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Mar 18 08:43:14 2009
10, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
10, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IDh8P7007648
10, 11 --     for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 08:43:11 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c5e6a9345c1a-at-[206.69.208.22]}
10, 11 -- Date: Wed, 18 Mar 2009 08:43:06 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: giulio.lamedica-at-libero.it (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
29, 25 -- From nizets2-at-yahoo.com Thu Mar 19 06:48:50 2009
29, 25 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
29, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBmnVl016611
29, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:48:49 -0500
29, 25 -- Received: (qmail 21371 invoked by uid 60001); 19 Mar 2009 11:48:48 -0000
29, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463328; bh=6y4wjuVLTZiqL+Itbc2QcdjOTRN3V8QxJpsjW8LUD24=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=GvT+cX8EZCIQZHd0ji+oYg07se1Tn8kBhrPOtMgJkD7fM2rRaRdNKAjgcgcAmNy7bLXAVDQmAXbO2LcNs64hi7Vyti8uoIxpzRihWASKKAQGctOoR4njW/mceBj58Vj6kcm2ObJ07fAAWoCHoYJwekc9ymbDiekKDs6hnRCGQdU=
29, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
29, 25 -- s=s1024; d=yahoo.com;
29, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
29, 25 -- b=Xdy4KPyg6M0Jj8GTaTZcNPwMOHWuknLHLNj7vSYtFpsN1TSoYG9Cz/v4eL9v0prLUJ+R0NWrchqyqCZAfwv5kqnWfEJWpKiIg1TM0HQdhlhF5Oz3BewgbqCGd7/EWbH+LJwgtGlciT5xAjIxFC7HWK0591YlIVon5WGNCtMslr8=;
29, 25 -- Message-ID: {37880.21296.qm-at-web110804.mail.gq1.yahoo.com}
29, 25 -- X-YMail-OSG: MZy0ywYVM1n2tpgwMjloT2EIKYDe6OY6B_9qhW6d6MF3e_F49pfXulaFDsM9ZWuolay0FHKAWbTpGFBLrEQIyRWh5SQ.dX0jXyK.CoX6ZC.fVY8bKi3BOFih_Lwd6kge6oTf3m.ltytLPzt1n6duV1VAeOM7WS4fGOHbukRfkMz2UvmaVuNlMPeHoNmdJOgQv2uwMhpYbO8r2MYaq4VcQBypDsw05xZUgUOIIlJk2A682meKA9mF.w34SOf0C_ueRzrNE..56Bex5kAl5RG_8XU-
29, 25 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:48:47 PDT
29, 25 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1
29, 25 -- References: {200903181351.n2IDpK4S014350-at-ns.microscopy.com}
29, 25 -- Date: Thu, 19 Mar 2009 04:48:47 -0700 (PDT)
29, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
29, 25 -- Subject: Re: [Microscopy] viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension
29, 25 -- To: giulio.lamedica-at-libero.it
29, 25 -- Cc: microscopy-at-microscopy.com
29, 25 -- In-Reply-To: {200903181351.n2IDpK4S014350-at-ns.microscopy.com}
29, 25 -- MIME-Version: 1.0
29, 25 -- Content-Type: text/plain; charset=iso-8859-1
29, 25 -- Content-Transfer-Encoding: 8bit
29, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBmnVl016611
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 19 Mar 2009 06:53:45 -0500
Subject: [Microscopy] What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Definitely viral particles.
I would suggest to use protocols to purify virus particles from cells in culture (pretty easy).
Then you can do whatever you want to identify them if you need/want to (PCR, ELISA...).

Stéphane



----- Original Message ----
X-from: "paul_hazelton-at-umanitoba.ca" {paul_hazelton-at-umanitoba.ca}
To: nizets2-at-yahoo.com
Sent: Wednesday, March 18, 2009 5:48:39 PM

Dotty, et al

Actually, the answer is in the original description of the specimens
"from an HIV positve patient".  Unfortunately the preparation in
Histogel has caused some deterioration in quality of specimen at the end
point.  What you are seeing is lentivirus particles.  given the specimen
source you can assume that they are the human version - ie HIV particles.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail:  paul_hazelton-at-umanitoba.ca
          paulhazelton-at-mts.net
Phone:    204-789-3313 (w);
          204-489-6924 (h)
Cell:    204-781-6982
Fax:      204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Mar 18 11:44:10 2009
6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34])
6, 20 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGi8D4002743
6, 20 --     for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:44:10 -0500
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 --     (authenticated bits=0)
6, 20 --     by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2IGi5lZ013388;
6, 20 --     Wed, 18 Mar 2009 11:44:05 -0500 (CDT)
6, 20 -- Message-ID: {49C124D4.8050302-at-umanitoba.ca}
6, 20 -- Date: Wed, 18 Mar 2009 11:44:04 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: dsoren-at-umich.edu
6, 20 -- Subject: Re: [Microscopy] Re: What is this critter?
6, 20 -- References: {200903181530.n2IFUrHM008486-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200903181530.n2IFUrHM008486-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================






==============================Original Headers==============================
21, 24 -- From nizets2-at-yahoo.com Thu Mar 19 06:53:44 2009
21, 24 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBripX024052
21, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:53:44 -0500
21, 24 -- Received: (qmail 1684 invoked by uid 60001); 19 Mar 2009 11:53:44 -0000
21, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463623; bh=U+D+lcVtgKRTB7sr2D+yajw8UP/A9hyN9nQBqk4SCJA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0aGXotpWnSboDeLkDcg6pklo7wwNtD7T8C+Nbd1nYQRX03g8uitRXaWtsJY8kr+8HsCZAKJfFbgGuOlohwNsbeTxzk/BVpig9QKVMNJhVwaNdEiJQWISmcUUeX5vkFKY4v6O3oipGahe1j1MRnXi5C1fCKC+QyjGmhZiUYpRqN4=
21, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
21, 24 -- s=s1024; d=yahoo.com;
21, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
21, 24 -- b=rBfBlIYR0RSjqJP4UAHTNmtErP+8B17lq2GjmCVZCL84oHzIyJ+rXIgzAuirwDmbAWYA8D6tJs6pUHV0VWq4Ozx+E/1ynutDBReuwl74OnUpFSSjQ/MwbT1lGvrZXpBkcji7rv2i+JhD0C+KnrTOooJSjDSJEUGbx/CmCftbew4=;
21, 24 -- Message-ID: {913802.715.qm-at-web110814.mail.gq1.yahoo.com}
21, 24 -- X-YMail-OSG: nHqaGNEVM1mA9jOpqRFNub8hyBjnn5irNtsQ4o1.XKTjfqJhJQ7OI0xu.fHOqNqBEhHzSba4NzN7Kpmfvn76Ax77z8ZVaWR2sMMetMbqYOOiQ.TTubfdsWQZ2Um9u360FPR1FDXAyL5.WMJnsYVPkhisw4MtVo4FY1mPd65uMX_Nskv50Cn_5J7BlNh14WnN8nEqPAT7sA5HDhgJVJ0MxSAZz7sJCi_eMppWwvgc_HzjPBKVrzxw48XhnsP9zNJ9cTwQlnJ.0a2donZQ8D9QLz5E865.RMW6qg--
21, 24 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:53:43 PDT
21, 24 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1
21, 24 -- References: {200903181648.n2IGmdJO009326-at-ns.microscopy.com}
21, 24 -- Date: Thu, 19 Mar 2009 04:53:43 -0700 (PDT)
21, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
21, 24 -- Subject: Re: [Microscopy] What is this critter?
21, 24 -- To: microscopy-at-microscopy.com
21, 24 -- In-Reply-To: {200903181648.n2IGmdJO009326-at-ns.microscopy.com}
21, 24 -- MIME-Version: 1.0
21, 24 -- Content-Type: text/plain; charset=iso-8859-1
21, 24 -- Content-Transfer-Encoding: 8bit
21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBripX024052
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 19 Mar 2009 06:57:39 -0500
Subject: [Microscopy] Re: TEM - Inviation to a new EM community web site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Completely agree.
No greetings and no signature. I don't go further, not even to the point of clicking on the link.

Stephane



----- Original Message ----
X-from: "petra.wahlbring-at-goodyear.com" {petra.wahlbring-at-goodyear.com}
To: nizets2-at-yahoo.com
Sent: Thursday, March 19, 2009 9:41:24 AM

Dear ...,

looking at your below linked site, I fail completely to see who is behind
that. An individual, a company? On your home page you say "I", in the
policy it says "we" and "our". Who?
The content of this page goes against zero at the moment, but the policy
makes clear that "you" will hold the right on everything published unless
otherwise noted by the contributor. You also intend to benefit from
commercial advertizements: "Please contact us if you wish to support this
site through advertisements."
With the anonymity practiced, I have the strong feeling that this site
intends to make profit from the knowledge of our community and our
willingness to share it. I personally think there are better places to
practice this (e.g. this list server).

Petra
__________________________________
Dr. Petra Wahlbring
Lead Engineer Analytical Test Lab
Goodyear S.A. Technical Center
Colmar-Berg, Luxembourg
e-mail: petra.wahlbring-at-goodyear.com
phone: +352 8199 3725 or GTN 631 3725
fax: +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be
copied or disseminated without the express written consent of The Goodyear
Tire & Rubber Company. -


                                                                         
            samwarren-at-emfocal                                           
            .com                                                         
                                                                        To
            03/19/09 04:33 AM        petra.wahlbring-at-goodyear.com       
                                                                        cc
                                                                         
            Please respond to                                    Subject
            samwarren-at-emfocal        [Microscopy] TEM - Inviation to a 
                  .com                new EM community web site         
                                                                         
                                                                         
                                                                         
                                                                         
                                                                         
                                                                         







----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America


This is an invitation to you to a brand new electron microscopy community
web site:
http://www.emfocal.com

This site focuses on information sharing and community. Our intention on
this site is to make it a “wikipedia-like†information repository: a user
collaborated information base about anything related to electron
microscopy, micro-analysis, applications, …

I invite you come to take a look of the site. If you like it, help us to
build it.

Feel free to forward this message.

Thank you,



==============================Original
Headers==============================
7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009
7, 31 -- Received: from outbound-mail-357.bluehost.com
(outbound-mail-357.bluehost.com [66.147.249.251])
7, 31 --          by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n2J3VJln009306
7, 31 --          for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009
22:31:19 -0500
7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37
-0000
7, 31 -- Received: from unknown (HELO host361.hostmonster.com)
(66.147.240.161)
7, 31 --  by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar
2009 03:26:37 -0000
7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default;
d=emfocal.com;
7, 31 --
h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;

7, 31 --          b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby
+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;

7, 31 -- Received: from localhost ([127.0.0.1]
helo=host361.hostmonster.com)
7, 31 --          by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 31 --          (envelope-from {samwarren-at-emfocal.com} )
7, 31 --          id 1Lk8yV-0006fA-BN
7, 31 --          for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19
-0600
7, 31 -- Received: from 124.192.231.32 ([124.192.231.32])
7, 31 --        (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 31 --        by host361.hostmonster.com with HTTP;
7, 31 --        Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Message-ID:
{1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com}
7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Subject: TEM - Inviation to a new EM community web site
7, 31 -- From: samwarren-at-emfocal.com
7, 31 -- To: Microscopy-at-Microscopy.Com
7, 31 -- User-Agent: SquirrelMail/1.4.13
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain;charset=iso-8859-1
7, 31 -- Content-Transfer-Encoding: 8bit
7, 31 -- X-Priority: 3 (Normal)
7, 31 -- Importance: Normal
7, 31 -- X-Identified-User:
{2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running
on server}
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 17 -- From petra.wahlbring-at-goodyear.com Thu Mar 19 03:35:47 2009
25, 17 -- Received: from emeam1.goodyear.com (emeam1.goodyear.com [57.67.226.7])
25, 17 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J8ZkcU030439
25, 17 --     for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 03:35:47 -0500
25, 17 -- In-Reply-To: {200903190333.n2J3XnG8014706-at-ns.microscopy.com}
25, 17 -- Subject: Re: [Microscopy] TEM - Inviation to a new EM community web site
25, 17 -- To: microscopy-at-microscopy.com
25, 17 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
25, 17 -- Message-ID: {OFBA11D716.1615D217-ONC125757E.002D858E-C125757E.002F2C9E-at-goodyear.com}
25, 17 -- From: petra.wahlbring-at-goodyear.com
25, 17 -- Date: Thu, 19 Mar 2009 09:35:16 +0100
25, 17 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 8.0.1|February 07, 2008) at
25, 17 --  03/19/2009 09:35:51 AM
25, 17 -- MIME-Version: 1.0
25, 17 -- Content-type: text/plain; charset=UTF-8
25, 17 -- Content-Transfer-Encoding: 8bit
25, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2J8ZkcU030439
==============================End of - Headers==============================






==============================Original Headers==============================
40, 24 -- From nizets2-at-yahoo.com Thu Mar 19 06:57:39 2009
40, 24 -- Received: from web110803.mail.gq1.yahoo.com (web110803.mail.gq1.yahoo.com [67.195.13.226])
40, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBvc8U002543
40, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:57:39 -0500
40, 24 -- Received: (qmail 53358 invoked by uid 60001); 19 Mar 2009 11:57:38 -0000
40, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463858; bh=ffKLAzvStBZJyCetr/0aw2VqXbo9Qe0ZyLnpW1hhIOI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=26vs9y9kG0malYz3m8EeP73F5h8AdEiX8/ym7Lh2Q66MJ3Go5x19lRaiv/MsVLDw9cXWsTqsQIralSBSQ7Xpodi1ysOojN8GT54DXT78rDHP0Md3i5oFxbEJKpWQ3HTTr9r5cTv5RCWP/1AXB9+yTmSDtP12xZHKVHa2RfSYyIU=
40, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
40, 24 -- s=s1024; d=yahoo.com;
40, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
40, 24 -- b=AznRG5wp5FzXl+unI8f1VbpmBnH1rdk7m9YxxHcFVEHSmMaAZtmGEbp+ipWJMHbXQn0uPeK77+A6sMa+8XiQvujvXErxvMfedgPqTedDiKWm6BkAuqV/FQXx4Lw3Oe14NeacdKVoKOLsdA3sNqJ0YDBkJdS5wrb0izxstWvlkI4=;
40, 24 -- Message-ID: {404152.53062.qm-at-web110803.mail.gq1.yahoo.com}
40, 24 -- X-YMail-OSG: JfVStY8VM1lUfslScPGCni1SKPjHUQZjwYJUHJI0iETc2GsV3yKkh9nxGqxHapoPHnSOYiBuExSmyFx7Gus7YRiwTGdKQfJ5PsTg7_n.DhENvuWG9Z_EaOaBff7v55BW6d2zKUjDCLiX0TVkUoD9WxRGeGSlfc_t9SYVoXK0.5m8rkV8vpghotNk6rxTfIKN_yJPcPh9L9GfNx_mAiSTawVlQzvWTnKYGz1ZK7X0xLRCm95wxh4oCHl6p7BActOh0u3wTK50_XdNlKQ3HKCf0Uw-
40, 24 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:57:38 PDT
40, 24 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1
40, 24 -- References: {200903190841.n2J8fO5c004588-at-ns.microscopy.com}
40, 24 -- Date: Thu, 19 Mar 2009 04:57:38 -0700 (PDT)
40, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
40, 24 -- Subject: Re: [Microscopy] Re: TEM - Inviation to a new EM community web site
40, 24 -- To: microscopy-at-microscopy.com
40, 24 -- In-Reply-To: {200903190841.n2J8fO5c004588-at-ns.microscopy.com}
40, 24 -- MIME-Version: 1.0
40, 24 -- Content-Type: text/plain; charset=utf-8
40, 24 -- Content-Transfer-Encoding: 8bit
40, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBvc8U002543
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 19 Mar 2009 07:41:24 -0500
Subject: [Microscopy] Re: TEM - Inviation to a new EM community web site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I decided to click. The legalese seems to simply recognize the fact that once you've put something on the web, it's there for everyone to use. Seems pretty straight-forward.

On the other hand, who is Sam Warren? It sounds like maybe I could do a search of Harvard's website and maybe find out, but why bother when nothing is offered for contact information concerning this "group" or "organization"? Does Harvard even know what he/they are up to? Sounds like he/they are concerned enough about being shut down to cover their derrieres, but don't want anyone else to have a clue as to who he/they are or what he/they are up to.

Right. I won't be back.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Thursday, March 19, 2009 8:00 AM
To: kenconverse-at-qualityimages.biz


Completely agree.
No greetings and no signature. I don't go further, not even to the point of clicking on the link.

Stephane



----- Original Message ----
X-from: "petra.wahlbring-at-goodyear.com" {petra.wahlbring-at-goodyear.com}
To: nizets2-at-yahoo.com
Sent: Thursday, March 19, 2009 9:41:24 AM

Dear ...,

looking at your below linked site, I fail completely to see who is behind
that. An individual, a company? On your home page you say "I", in the
policy it says "we" and "our". Who?
The content of this page goes against zero at the moment, but the policy
makes clear that "you" will hold the right on everything published unless
otherwise noted by the contributor. You also intend to benefit from
commercial advertizements: "Please contact us if you wish to support this
site through advertisements."
With the anonymity practiced, I have the strong feeling that this site
intends to make profit from the knowledge of our community and our
willingness to share it. I personally think there are better places to
practice this (e.g. this list server).

Petra
__________________________________
Dr. Petra Wahlbring
Lead Engineer Analytical Test Lab
Goodyear S.A. Technical Center
Colmar-Berg, Luxembourg
e-mail: petra.wahlbring-at-goodyear.com
phone: +352 8199 3725 or GTN 631 3725
fax: +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be
copied or disseminated without the express written consent of The Goodyear
Tire & Rubber Company. -


                                    Â
      samwarren-at-emfocal                     Â
      .com                            Â
                                    To
      03/19/09 04:33 AM    petra.wahlbring-at-goodyear.com   Â
                                    cc
                                    Â
      Please respond to                  Subject
      samwarren-at-emfocal    [Microscopy] TEM - Inviation to aÂ
         .com        new EM community web site    Â
                                    Â
                                    Â
                                    Â
                                    Â
                                    Â
                                    Â







----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor:Â The Microscopy Society of America


This is an invitation to you to a brand new electron microscopy community
web site:
http://www.emfocal.com

This site focuses on information sharing and community. Our intention on
this site is to make it a “wikipedia-like†information repository: a user
collaborated information base about anything related to electron
microscopy, micro-analysis, applications, …

I invite you come to take a look of the site. If you like it, help us to
build it.

Feel free to forward this message.

Thank you,



==============================Original
Headers==============================
7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009
7, 31 -- Received: from outbound-mail-357.bluehost.com
(outbound-mail-357.bluehost.com [66.147.249.251])
7, 31 --Â Â Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n2J3VJln009306
7, 31 --Â Â Â Â Â for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009
22:31:19 -0500
7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37
-0000
7, 31 -- Received: from unknown (HELO host361.hostmonster.com)
(66.147.240.161)
7, 31 --Â by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar
2009 03:26:37 -0000
7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default;
d=emfocal.com;
7, 31 --
h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;

7, 31 --Â Â Â Â Â b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby
+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;

7, 31 -- Received: from localhost ([127.0.0.1]
helo=host361.hostmonster.com)
7, 31 --Â Â Â Â Â by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 31 --Â Â Â Â Â (envelope-from {samwarren-at-emfocal.com} )
7, 31 --Â Â Â Â Â id 1Lk8yV-0006fA-BN
7, 31 --Â Â Â Â Â for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19
-0600
7, 31 -- Received: from 124.192.231.32 ([124.192.231.32])
7, 31 --Â Â Â Â (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 31 --Â Â Â Â by host361.hostmonster.com with HTTP;
7, 31 --Â Â Â Â Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Message-ID:
{1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com}
7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Subject: TEM - Inviation to a new EM community web site
7, 31 -- From: samwarren-at-emfocal.com
7, 31 -- To: Microscopy-at-Microscopy.Com
7, 31 -- User-Agent: SquirrelMail/1.4.13
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain;charset=iso-8859-1
7, 31 -- Content-Transfer-Encoding: 8bit
7, 31 -- X-Priority: 3 (Normal)
7, 31 -- Importance: Normal
7, 31 -- X-Identified-User:
{2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running
on server}
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 17 -- From petra.wahlbring-at-goodyear.com Thu Mar 19 03:35:47 2009
25, 17 -- Received: from emeam1.goodyear.com (emeam1.goodyear.com [57.67.226.7])
25, 17 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J8ZkcU030439
25, 17 -- Â Â Â for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 03:35:47 -0500
25, 17 -- In-Reply-To: {200903190333.n2J3XnG8014706-at-ns.microscopy.com}
25, 17 -- Subject: Re: [Microscopy] TEM - Inviation to a new EM community web site
25, 17 -- To: microscopy-at-microscopy.com
25, 17 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
25, 17 -- Message-ID: {OFBA11D716.1615D217-ONC125757E.002D858E-C125757E.002F2C9E-at-goodyear.com}
25, 17 -- From: petra.wahlbring-at-goodyear.com
25, 17 -- Date: Thu, 19 Mar 2009 09:35:16 +0100
25, 17 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 8.0.1|February 07, 2008) at
25, 17 --Â 03/19/2009 09:35:51 AM
25, 17 -- MIME-Version: 1.0
25, 17 -- Content-type: text/plain; charset=UTF-8
25, 17 -- Content-Transfer-Encoding: 8bit
25, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2J8ZkcU030439
==============================End of - Headers==============================






==============================Original Headers==============================
40, 24 -- From nizets2-at-yahoo.com Thu Mar 19 06:57:39 2009
40, 24 -- Received: from web110803.mail.gq1.yahoo.com (web110803.mail.gq1.yahoo.com [67.195.13.226])
40, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBvc8U002543
40, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:57:39 -0500
40, 24 -- Received: (qmail 53358 invoked by uid 60001); 19 Mar 2009 11:57:38 -0000
40, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463858; bh=ffKLAzvStBZJyCetr/0aw2VqXbo9Qe0ZyLnpW1hhIOI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=26vs9y9kG0malYz3m8EeP73F5h8AdEiX8/ym7Lh2Q66MJ3Go5x19lRaiv/MsVLDw9cXWsTqsQIralSBSQ7Xpodi1ysOojN8GT54DXT78rDHP0Md3i5oFxbEJKpWQ3HTTr9r5cTv5RCWP/1AXB9+yTmSDtP12xZHKVHa2RfSYyIU=
40, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
40, 24 -- s=s1024; d=yahoo.com;
40, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
40, 24 -- b=AznRG5wp5FzXl+unI8f1VbpmBnH1rdk7m9YxxHcFVEHSmMaAZtmGEbp+ipWJMHbXQn0uPeK77+A6sMa+8XiQvujvXErxvMfedgPqTedDiKWm6BkAuqV/FQXx4Lw3Oe14NeacdKVoKOLsdA3sNqJ0YDBkJdS5wrb0izxstWvlkI4=;
40, 24 -- Message-ID: {404152.53062.qm-at-web110803.mail.gq1.yahoo.com}
40, 24 -- X-YMail-OSG: JfVStY8VM1lUfslScPGCni1SKPjHUQZjwYJUHJI0iETc2GsV3yKkh9nxGqxHapoPHnSOYiBuExSmyFx7Gus7YRiwTGdKQfJ5PsTg7_n.DhENvuWG9Z_EaOaBff7v55BW6d2zKUjDCLiX0TVkUoD9WxRGeGSlfc_t9SYVoXK0.5m8rkV8vpghotNk6rxTfIKN_yJPcPh9L9GfNx_mAiSTawVlQzvWTnKYGz1ZK7X0xLRCm95wxh4oCHl6p7BActOh0u3wTK50_XdNlKQ3HKCf0Uw-
40, 24 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:57:38 PDT
40, 24 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1
40, 24 -- References: {200903190841.n2J8fO5c004588-at-ns.microscopy.com}
40, 24 -- Date: Thu, 19 Mar 2009 04:57:38 -0700 (PDT)
40, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
40, 24 -- Subject: Re: [Microscopy] Re: TEM - Inviation to a new EM community web site
40, 24 -- To: microscopy-at-microscopy.com
40, 24 -- In-Reply-To: {200903190841.n2J8fO5c004588-at-ns.microscopy.com}
40, 24 -- MIME-Version: 1.0
40, 24 -- Content-Type: text/plain; charset=utf-8
40, 24 -- Content-Transfer-Encoding: 8bit
40, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBvc8U002543
==============================End of - Headers==============================




==============================Original Headers==============================
54, 25 -- From kenconverse-at-qualityimages.biz Thu Mar 19 07:41:24 2009
54, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
54, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JCfNbe026683
54, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 07:41:23 -0500
54, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
54, 25 -- with ESMTP
54, 25 -- id {20090319124123.HHKJ133.cdptpa-omta04.mail.rr.com-at-Ken} ;
54, 25 -- Thu, 19 Mar 2009 12:41:23 +0000
54, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
54, 25 -- To: {nizets2-at-yahoo.com} , "MSA Listserver" {microscopy-at-microscopy.com}
54, 25 -- Subject: RE: [Microscopy] TEM - Inviation to a new EM community web site
54, 25 -- Date: Thu, 19 Mar 2009 08:41:16 -0400
54, 25 -- Message-ID: {F74AE1165E434E32ADE803295B548BD7-at-Ken}
54, 25 -- MIME-Version: 1.0
54, 25 -- Content-Type: text/plain;
54, 25 -- charset="UTF-8"
54, 25 -- X-Priority: 3 (Normal)
54, 25 -- X-MSMail-Priority: Normal
54, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
54, 25 -- Importance: Normal
54, 25 -- Thread-Index: AcmoijtDcDQQvUfaTta6HFzmj1iVmAABDkjw
54, 25 -- In-Reply-To: {200903191159.n2JBxw6x010948-at-ns.microscopy.com}
54, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
54, 25 -- Content-Transfer-Encoding: 8bit
54, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JCfNbe026683
==============================End of - Headers==============================




From: StevenLe-at-BaylorHealth.edu
Date: Thu, 19 Mar 2009 08:39:21 -0500
Subject: [Microscopy] TEM - Inviation to a new EM community web site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello lister's

This site is registered to Shixin Wang. Only reference I found was for a post doc at U. Mich., but the site indicated that the fellowship ended in 2000, so I can not say for sure whether it is the same person, or not.

Not sure who Sam is, or if you should trust him. Probably not, I do not think he uses a spell checker.

Steve


Steven Lee
Chief Technologist
Electron Microscopy Laboratory
Baylor University Medical Center
3500 Gaston Ave
Dallas, TX 75246
6 214.820.3302
Ê 214.820.4110
2 stevenle-at-baylorhealth.edu


-----Original Message-----
X-from: samwarren-at-emfocal.com [mailto:samwarren-at-emfocal.com]
Sent: Wednesday, March 18, 2009 10:35 PM
To: Lee, Steven

This is an invitation to you to a brand new electron microscopy community web site:
http://www.emfocal.com

This site focuses on information sharing and community. Our intention on this site is to make it a "wikipedia-like" information repository: a user collaborated information base about anything related to electron microscopy, micro-analysis, applications, ...

I invite you come to take a look of the site. If you like it, help us to build it.

Feel free to forward this message.

Thank you,



==============================Original Headers==============================
7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009 7, 31 -- Received: from outbound-mail-357.bluehost.com (outbound-mail-357.bluehost.com [66.147.249.251])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J3VJln009306
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 22:31:19 -0500
7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37 -0000 7, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161)
7, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar 2009 03:26:37 -0000
7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com;
7, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
7, 31 -- b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;
7, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com)
7, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 31 -- (envelope-from {samwarren-at-emfocal.com} )
7, 31 -- id 1Lk8yV-0006fA-BN
7, 31 -- for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19 -0600
7, 31 -- Received: from 124.192.231.32 ([124.192.231.32])
7, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 31 -- by host361.hostmonster.com with HTTP;
7, 31 -- Wed, 18 Mar 2009 21:31:19 -0600 (MDT)
7, 31 -- Message-ID: {1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com}
7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT) 7, 31 -- Subject: TEM - Inviation to a new EM community web site 7, 31 -- From: samwarren-at-emfocal.com 7, 31 -- To: Microscopy-at-Microscopy.Com 7, 31 -- User-Agent: SquirrelMail/1.4.13 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain;charset=iso-8859-1 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-Priority: 3 (Normal) 7, 31 -- Importance: Normal 7, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================

**********************************************************************
This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy-at-baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.


==============================Original Headers==============================
22, 30 -- From StevenLe-at-BaylorHealth.edu Thu Mar 19 08:39:20 2009
22, 30 -- Received: from bhdappagnt02.baylorhealth.edu (mailhost1.bhcs.com [65.248.93.160])
22, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JDdH5a010099
22, 30 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 08:39:19 -0500
22, 30 -- Received: from BHDAEXIMS01.bhcs.pvt ([10.5.12.120])
22, 30 -- by bhdappagnt02.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id n2JDdFZ6030680
22, 30 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 08:39:15 -0500
22, 30 -- X-TM-IMSS-Message-ID: {1e54a39e000348d8-at-bhcs.pvt}
22, 30 -- Received: from BHDAEXHT02.bhcs.pvt ([10.5.12.191]) by bhcs.pvt ([10.5.12.120]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 1e54a39e000348d8 for {StevenLe-at-BaylorHealth.edu} ; Thu, 19 Mar 2009 08:39:14 -0600
22, 30 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT02.bhcs.pvt
22, 30 -- ([10.5.12.191]) with mapi; Thu, 19 Mar 2009 08:39:14 -0500
22, 30 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu}
22, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
22, 30 -- Date: Thu, 19 Mar 2009 08:39:13 -0500
22, 30 -- Subject: RE: [Microscopy] TEM - Inviation to a new EM community web site
22, 30 -- Thread-Topic: [Microscopy] TEM - Inviation to a new EM community web site
22, 30 -- Thread-Index: AcmoQ6Cxnf+M1eAUSYKElT/8kHoF2QAU4Qiw
22, 30 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0CD82C4AB7-at-BHDAEXVM32.bhcs.pvt}
22, 30 -- References: {200903190334.n2J3YZrD016771-at-ns.microscopy.com}
22, 30 -- In-Reply-To: {200903190334.n2J3YZrD016771-at-ns.microscopy.com}
22, 30 -- Accept-Language: en-US
22, 30 -- Content-Language: en-US
22, 30 -- X-MS-Has-Attach:
22, 30 -- X-MS-TNEF-Correlator:
22, 30 -- acceptlanguage: en-US
22, 30 -- Content-Type: text/plain; charset="iso-8859-1"
22, 30 -- MIME-Version: 1.0
22, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-03-19_09:2009-03-19,2009-03-19,2009-03-19 signatures=0
22, 30 -- Content-Transfer-Encoding: 8bit
22, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JDdH5a010099
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Thu, 19 Mar 2009 11:00:35 -0500
Subject: [Microscopy] EM room flooring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., folks quick question.

For flooring in an EM Room looking for opinions would a static
control flooring be benificial? And should it be dissipative or
conductive?

I have no experience with eitehr one in an EM Room. Anyone want to
vocice an opinion?
Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


==============================Original Headers==============================
4, 22 -- From edelmare-at-muohio.edu Thu Mar 19 11:00:34 2009
4, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JG0UWi028370
4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 11:00:33 -0500
4, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
4, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n2JG0RlX024462
4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 12:00:27 -0400
4, 22 -- Received: from [192.168.1.23] ([134.53.14.105])
4, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n2JG0OXa021631
4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 12:00:24 -0400
4, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
4, 22 -- To: microscopy-at-Microscopy.com
4, 22 -- Date: Thu, 19 Mar 2009 12:00:22 -0400
4, 22 -- MIME-Version: 1.0
4, 22 -- Subject: EM room flooring
4, 22 -- Message-ID: {49C233D6.31646.13A14A3E-at-edelmare.muohio.edu}
4, 22 -- Priority: normal
4, 22 -- X-mailer: Pegasus Mail for Windows (4.41)
4, 22 -- Content-type: text/plain; charset=US-ASCII
4, 22 -- Content-transfer-encoding: 7BIT
4, 22 -- Content-description: Mail message body
4, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Thu, 19 Mar 2009 12:57:30 -0500
Subject: [Microscopy] Re: EM room flooring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard

we had conductive flooring laid, but it was so long ago that I can't
remember the type. I do recall it was the cheaper type because the
very best "electronic industry" type wasn't needed.

One thing to be careful about is that if you still handle liquid
nitrogen it can crack the vinyl types of floor quite quickly so it's
worth having an area reserved for pouring which is not easily damaged.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: edelmare-at-muohio.edu

Just wondering - wouldn't polished concrete, recently suggested by someone
as good floor option for LN2 resistance, also be sufficiently dissipative
for ESD purposes? Of cause resistivity of such floor will somewhat vary,
with time and depending on air humidity etc..., but some references suggest
resistance of cured concrete to be around 250 Ohm*M, which is very
conductive...

Valery Ray

============================
www.partbeamsystech.com
PBS&T, MEO Engineering Co, Inc.
290 Broadway St., Suite 298
Methuen, MA 01844
Phone: (978) 296-5063


-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk
[mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Thursday, March 19, 2009 12:35 PM
To: vray-at-partbeamsystech.com

Richard

we had conductive flooring laid, but it was so long ago that I can't
remember the type. I do recall it was the cheaper type because the
very best "electronic industry" type wasn't needed.

One thing to be careful about is that if you still handle liquid
nitrogen it can crack the vinyl types of floor quite quickly so it's
worth having an area reserved for pouring which is not easily damaged.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: edelmare-at-muohio.edu

Just to confirm- we are very happy with these polished concrete
floors (well, they are little unforgiving with dropped glassware) and
we have had zero incidents with regard to static buildup which is a
problem in Oregon in the winter. Compared to the static problems we
had in our old lab with sealed concrete (and indoor carpeting!) we
are much happier.
john

At 10:37 AM 3/19/2009, vray-at-partbeamsystech.com wrote:

} Just wondering - wouldn't polished concrete, recently suggested by someone
} as good floor option for LN2 resistance, also be sufficiently dissipative
} for ESD purposes? Of cause resistivity of such floor will somewhat vary,
} with time and depending on air humidity etc..., but some references suggest
} resistance of cured concrete to be around 250 Ohm*M, which is very
} conductive...
}
} Valery Ray
}
} ============================
} www.partbeamsystech.com
} PBS&T, MEO Engineering Co, Inc.
} 290 Broadway St., Suite 298
} Methuen, MA 01844
} Phone: (978) 296-5063
}
}
} -----Original Message-----
} X-from: malcolm.haswell-at-sunderland.ac.uk
} [mailto:malcolm.haswell-at-sunderland.ac.uk]
} Sent: Thursday, March 19, 2009 12:35 PM
} To: vray-at-partbeamsystech.com
} Subject: [Microscopy] Re: EM room flooring
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
4, 22 -- From donovan-at-uoregon.edu Thu Mar 19 12:57:30 2009
4, 22 -- Received: from smtp.uoregon.edu (mserv5.uoregon.edu [128.223.142.42])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JHvTTH008264
4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 12:57:29 -0500
4, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
4, 22 -- (authenticated bits=0)
4, 22 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n2JHvT6U027068
4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
4, 22 -- Thu, 19 Mar 2009 10:57:29 -0700
4, 22 -- Message-Id: {200903191757.n2JHvT6U027068-at-smtp.uoregon.edu}
4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
4, 22 -- Date: Thu, 19 Mar 2009 10:57:15 -0700
4, 22 -- To: vray-at-partbeamsystech.com
4, 22 -- From: John Donovan {donovan-at-uoregon.edu}
4, 22 -- Subject: Re: [Microscopy] EM room flooring
4, 22 -- Cc: microscopy-at-microscopy.com
4, 22 -- In-Reply-To: {200903191737.n2JHbRRA001449-at-ns.microscopy.com}
4, 22 -- References: {200903191737.n2JHbRRA001449-at-ns.microscopy.com}
4, 22 -- Mime-Version: 1.0
4, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
4, 22 -- X-Virus-Scanned: ClamAV 0.94.2/9141/Thu Mar 19 09:24:25 2009 on mserv5
4, 22 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: dsoren-at-umich.edu
Date: Thu, 19 Mar 2009 13:16:20 -0500
Subject: [Microscopy] Critter ID

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Many thanks to all who replied to my question about the identity of
our critter. Many think it is a virus. Cytomegalovirus, Herpes,
HIV, and paramyxavirus were suggested. Others think that it is not a
virus, but perhaps yeast, mycoplasma, red blood cells coated with
Histogel, or some other unidentified organism. Protein
agglomerations, residual bodies, and calcium nodules were also
mentioned.

I agree with those who mentioned a uranyl acetate crystal
contamination. I had used phosphate buffer, though I did do three
ddH2O rinses before en bloc staining. Still, it does look like UAc
contamination. On the next round I will switch to cacodylate buffer
and also leave out the en bloc staining step to remove all
possibility of UAc precipitation. That should clear things up.

I will post the new and improved images from the next round as soon
as I have them.

For those of you who might be tuning in late to this discussion, here
is the link to the images that we have been talking about.

http://www.med.umich.edu/cdb/mil/docs/temimages.html

Thanks again!

Dotty



Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
A807 BSRB
109 Zina Pitcher Place
Ann Arbor, MI 48109-2200
(734)763-1170
FAX (734)763-1166



==============================Original Headers==============================
13, 16 -- From dsoren-at-umich.edu Thu Mar 19 13:16:19 2009
13, 16 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161])
13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JIGJWE022384
13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 13:16:19 -0500
13, 16 -- Received: FROM [10.21.131.86] (host-18.subnet-17.med.umich.edu [141.214.17.18])
13, 16 -- BY tombraider.mr.itd.umich.edu ID 49C28BB7.86E97.5890 ;
13, 16 -- 19 Mar 2009 14:15:19 -0400
13, 16 -- Mime-Version: 1.0 (Apple Message framework v753.1)
13, 16 -- Content-Transfer-Encoding: 7bit
13, 16 -- Message-Id: {44315564-5BB5-4A0E-A1D7-56C3325DC4EB-at-umich.edu}
13, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
13, 16 -- To: microscopy-at-microscopy.com
13, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
13, 16 -- Subject: Critter ID
13, 16 -- Date: Thu, 19 Mar 2009 14:15:18 -0400
13, 16 -- X-Mailer: Apple Mail (2.753.1)
==============================End of - Headers==============================




From: sappleby-at-mines.edu
Date: Thu, 19 Mar 2009 14:59:34 -0500
Subject: [Microscopy] fine-grained coal sample prep for SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,
I'm trying to mount fine-grained coal particles (-125 um) for EDS-based
automated mineral analysis. In a first attempt I used epoxy resin
(Struers EpoFix), but the BSE intensity of epoxy and coal are too
similar, which means the system can't differentiate easily between the
two. After doing some background reading I tried mounting the material
in molten Carnauba wax. Problem is the polishing is difficult as the wax
is extremely soft and the system is taking forever to pump down (I'm not
convinced yet it'll ever reach the required vacuum). I'm sure there must
be working protocols out there. Does anyone have any recommendations or
ideas?

Thanks,
Sarah Appleby

--
*********************************
Dr. Sarah Appleby
Technical Scientist
Advanced Mineralogy Research Center
Colorado School of Mines
1310 Maple Street
Golden, CO 80401, USA

office: 303-384-2487
fax: 303-384-2499
email: sappleby-at-mines.edu

*********************************



==============================Original Headers==============================
7, 16 -- From sappleby-at-mines.edu Thu Mar 19 14:59:33 2009
7, 16 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU [138.67.130.5])
7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JJxXIb006242
7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:59:33 -0500
7, 16 -- Received: from [127.0.0.1] (grlmineral03.Mines.EDU [138.67.44.224])
7, 16 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id n2JJxWs9006009
7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 13:59:32 -0600
7, 16 -- Message-ID: {49C2A424.3040600-at-mines.edu}
7, 16 -- Date: Thu, 19 Mar 2009 13:59:32 -0600
7, 16 -- From: Sarah Appleby {sappleby-at-mines.edu}
7, 16 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213)
7, 16 -- MIME-Version: 1.0
7, 16 -- To: Microscopy-at-Microscopy.Com
7, 16 -- Subject: fine-grained coal sample prep for SEM?
7, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 16 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Thu, 19 Mar 2009 17:34:27 -0500
Subject: [Microscopy] What is this critter?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

It looks like calcification or some similar precipitate surrounding the
central granular mass. I don't think the roundish particles are regular
enough to be viruses.
I've seen similar electron-dense material in numerous kidney biopsies which
I have always interpreted as calcification within the tissue.

Regards,

John Brealey





Electron Microscope Unit, Surgical Pathology

SA Pathology

Queen Elizabeth Hospital

Woodville, 5011

AUSTRALIA




==============================Original Headers==============================
13, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Mar 19 17:34:27 2009
13, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JMYP6W023471
13, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 17:34:26 -0500
13, 27 -- X-IronPort-AV: E=Sophos;i="4.38,391,1233495000";
13, 27 -- d="scan'208";a="15802510"
13, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
13, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Mar 2009 09:04:09 +1030
13, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
13, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
13, 27 -- 8.1.263.0; Fri, 20 Mar 2009 09:03:10 +1030
13, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
13, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 142362EC078 for
13, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 09:04:09 +1030 (CST)
13, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
13, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
13, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
13, 27 -- Subject: What is this critter?
13, 27 -- Date: Fri, 20 Mar 2009 09:04:08 +1030
13, 27 -- Organization: IMVS
13, 27 -- Message-ID: {000301c9a8e2$d13115a0$7d8a140a-at-41347i}
13, 27 -- MIME-Version: 1.0
13, 27 -- Content-Type: text/plain; charset="us-ascii"
13, 27 -- Content-Transfer-Encoding: 7bit
13, 27 -- X-Mailer: Microsoft Office Outlook 11
13, 27 -- Thread-Index: Acmo4tEKZEE6a2BxTNWZWykLt8BukQ==
13, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Thu, 19 Mar 2009 21:29:03 -0500
Subject: [Microscopy] Re: fine-grained coal sample prep for SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sarah,

I don't know the answer to your particular problem but in other cases that
sound similar to what you are describing, I have found using a polyester
mount material works.

You can try:

http://www.extec.com/mounting/cold-mounting-systems.php

Scroll down to the bottom of their list and find their polyester mount
material. Part 14675 and 14685 for the smaller size. I don't know of
another source but I'm sure they exist.

standard disclaimer: I have no interest in this company except as a
satisfied customer...

dj

On Thu, 19 Mar 2009, sappleby-at-mines.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear all,
} I'm trying to mount fine-grained coal particles (-125 um) for EDS-based
} automated mineral analysis. In a first attempt I used epoxy resin
} (Struers EpoFix), but the BSE intensity of epoxy and coal are too
} similar, which means the system can't differentiate easily between the
} two. After doing some background reading I tried mounting the material
} in molten Carnauba wax. Problem is the polishing is difficult as the wax
} is extremely soft and the system is taking forever to pump down (I'm not
} convinced yet it'll ever reach the required vacuum). I'm sure there must
} be working protocols out there. Does anyone have any recommendations or
} ideas?
}
} Thanks,
} Sarah Appleby
}
} --
} *********************************
} Dr. Sarah Appleby
} Technical Scientist
} Advanced Mineralogy Research Center
} Colorado School of Mines
} 1310 Maple Street
} Golden, CO 80401, USA
}
} office: 303-384-2487
} fax: 303-384-2499
} email: sappleby-at-mines.edu
}
} *********************************
}
}
}
} ==============================Original Headers==============================
} 7, 16 -- From sappleby-at-mines.edu Thu Mar 19 14:59:33 2009
} 7, 16 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU [138.67.130.5])
} 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JJxXIb006242
} 7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:59:33 -0500
} 7, 16 -- Received: from [127.0.0.1] (grlmineral03.Mines.EDU [138.67.44.224])
} 7, 16 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id n2JJxWs9006009
} 7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 13:59:32 -0600
} 7, 16 -- Message-ID: {49C2A424.3040600-at-mines.edu}
} 7, 16 -- Date: Thu, 19 Mar 2009 13:59:32 -0600
} 7, 16 -- From: Sarah Appleby {sappleby-at-mines.edu}
} 7, 16 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213)
} 7, 16 -- MIME-Version: 1.0
} 7, 16 -- To: Microscopy-at-Microscopy.Com
} 7, 16 -- Subject: fine-grained coal sample prep for SEM?
} 7, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 16 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
9, 21 -- From dljones-at-bestweb.net Thu Mar 19 21:29:03 2009
9, 21 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2K2T370010501
9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 21:29:03 -0500
9, 21 -- Received: from Pappee (ool-44c57e9d.dyn.optonline.net [68.197.126.157])
9, 21 -- by mta2.srv.hcvlny.cv.net
9, 21 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007))
9, 21 -- with ESMTP id {0KGS00GH69KF62X0-at-mta2.srv.hcvlny.cv.net} for
9, 21 -- microscopy-at-microscopy.com; Thu, 19 Mar 2009 22:29:03 -0400 (EDT)
9, 21 -- Date: Thu, 19 Mar 2009 22:29:06 -0400 (Eastern Daylight Time)
9, 21 -- From: "David L. Jones" {dljones-at-bestweb.net}
9, 21 -- Subject: Re: [Microscopy] fine-grained coal sample prep for SEM?
9, 21 -- In-reply-to: {200903192002.n2JK2rCk011513-at-ns.microscopy.com}
9, 21 -- X-X-Sender: dljones-at-imap.bestweb.net
9, 21 -- To: sappleby-at-mines.edu
9, 21 -- Cc: microscopy-at-microscopy.com
9, 21 -- Message-id: {Pine.WNT.4.64.0903192228550.5576-at-Pappee}
9, 21 -- MIME-version: 1.0
9, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed
9, 21 -- Content-transfer-encoding: 7BIT
9, 21 -- References: {200903192002.n2JK2rCk011513-at-ns.microscopy.com}
==============================End of - Headers==============================




From: samwarren-at-emfocal.com
Date: Fri, 20 Mar 2009 04:16:59 -0500
Subject: [Microscopy] TEM-reply to Invitation to a new EM community site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It seems some people concerned about the legal terms of the site. To tell
you the truth, I am total new in starting a community site. I found that
term of use which is free to use. So I modified it a little bit and used
it. I definitely will write one of my own. All want to say about the terms
of use are: (1) Do not abuse the site; (2) I am not responsible on what
you post; (3) Copyright belongs to the individual users; (4) I hold the
right to modify/delete some contents which may be improper to keep.
I may need to add some more. All mostly to cover my ass if there are some
legal issues come up.
Yes, the terms of use was mostly copied from Harvard website. I just found
"Harvard" name was still left in the terms of use. i just removed it. It
is not related to Harvard at all. I just borrowed their writing.
Believe, my primary reason for setting this up is to serve the community.
I have no problem to show my identity. But I am worried showing my real
name may turn someone away from using it. I myself would not like to post
much on some individual research group site. I keep anonymous just to make
you think I am nobody. Don't care about me. This site is for everyone to
use. I believe a site making no money will not be long-lived. I want the
information posted there to stay for hundreds of years.
Think about it, what harm can it do to me if I show myself up? None. It is
not a site against any laws. Why would someone want to shut me up? I think
this site is straight forward enough. It is for everyone to use. Any
individual research group site will not attract world-wide participation.
Do you believe a post-doc or a graduate student can keep the site alive
long? Do you all want to post on FEI site, JEOL site? The only way to make
the site long live is to commercialize it and make it a dedicated
community server.
I spent most of my recent time on building the site, not on policies. I
welcome you comments and wish you can help me out to straighten this site
out. I think the functionality of the site is ok. But I am really not good
on setting up policies. But I do need legal cover for myself.
Again, I sepnt some time on the site structure, but not on the content. I
intent to post some of my writting there. I may single-handedly write some
TEM, EELS, STEM, FIB, SEM, EDS tutorials. This site may end up mostly just
like that. But I encourage your contribution. I have seen many valuable
information scattered around list servers, and emails. I just wish they
can accumulate at a dedicated place and useful for a long time.
I maybe wrong about keeping the site owner anonymous. It can change if
most of you demand it.
At my work, I helped many people on TEM and other techniques. I felt some
of my answers may be written as a FAQ for my colleagues to see. Then I
came up an idea about building a TEM-FAQ site. I started searching the
ways to build the site and I found out I can do a lot more than a FAQ. And
we need more than a FAQ-site. So, after several months, I came up with
this:
http://www.emfocal.com
This site is in fact a personal site. But if this site can gain some money
it can live longer and not limited by me as a single person. You see many
forum, blog, community sites around. My intention is the same as most of
them.
Enough said for now. It will be too long for people to read.

Thank you for you attention.



==============================Original Headers==============================
4, 31 -- From samwarren-at-emfocal.com Fri Mar 20 04:16:59 2009
4, 31 -- Received: from outbound-mail-352.bluehost.com (outbound-mail-352.bluehost.com [66.147.249.13])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2K9Gwka000946
4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Mar 2009 04:16:58 -0500
4, 31 -- Received: (qmail 11610 invoked by uid 0); 20 Mar 2009 09:12:13 -0000
4, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161)
4, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 20 Mar 2009 09:12:13 -0000
4, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com;
4, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
4, 31 -- b=XNU9zD7exEBRUvI//bp7eRTNLZVIEWnKeStnLA9+GjB3MG4U/BaUv3xAvXIQ9468Xypa9rCpHaEmQGoIakW/RjLZ4wwnzEmv7IwkTZ78A9+WXBqQPWp6W9uOETm+YLcL;
4, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com)
4, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
4, 31 -- (envelope-from {samwarren-at-emfocal.com} )
4, 31 -- id 1LkaqX-0005VN-8B
4, 31 -- for Microscopy-at-Microscopy.Com; Fri, 20 Mar 2009 03:16:58 -0600
4, 31 -- Received: from 124.192.231.2 ([124.192.231.2])
4, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
4, 31 -- by host361.hostmonster.com with HTTP;
4, 31 -- Fri, 20 Mar 2009 03:16:57 -0600 (MDT)
4, 31 -- Message-ID: {1675.124.192.231.2.1237540617.squirrel-at-host361.hostmonster.com}
4, 31 -- Date: Fri, 20 Mar 2009 03:16:57 -0600 (MDT)
4, 31 -- Subject: TEM-reply to Invitation to a new EM community site
4, 31 -- From: samwarren-at-emfocal.com
4, 31 -- To: Microscopy-at-Microscopy.Com
4, 31 -- User-Agent: SquirrelMail/1.4.13
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;charset=iso-8859-1
4, 31 -- Content-Transfer-Encoding: 8bit
4, 31 -- X-Priority: 3 (Normal)
4, 31 -- Importance: Normal
4, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server}
==============================End of - Headers==============================




From: mike.net.mo-at-gmail.com
Date: Fri, 20 Mar 2009 07:01:16 -0500
Subject: [Microscopy] viaWWW: Change the contrast between the outer and inner of the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard

we had the conductive floor in the microscope room and wooden floor
where the liquid nitrogen was stored so it seemed a good arrangement.
The conductive floor was easy to keep clean, too.

We certainly never saw any problems with static.

Malcolm

Malcolm Haswell
Electron Microscope
Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1
3SD
UK


----- Original Message -----
X-from: "Richard E. Edelmann" {edelmare-at-muohio.edu}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mike.net.mo-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mike.net.mo-at-gmail.com
Name: Xmfan

Organization: USTC

Title-Subject: [Filtered] Change the contrast between the outer and
inner of the diffraction circle to a linear value

Question: In the experiment we imaged a silica
bead(2um) , and we gained the image of a silica bead
which was a diffraction ring, and the brightness of the diffraction
ring changed with the distance. (darker in the circle and
lower outside the circle). Now we want to change the contrast
between the outer and inner of the diffraction circle to a linear
value so that I can know the positon of the bead, I don't know which
lens or optical parts can be used to achieve this function.

Login Host: 159.226.118.36
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Mar 20 07:01:16 2009
6, 12 -- Received: from [192.168.1.101] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KC1Dg6000889
6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 07:01:15 -0500
6, 12 -- Mime-Version: 1.0
6, 12 -- Message-Id: {p06240800c5e935ee36c5-at-[10.0.239.114]}
6, 12 -- Date: Fri, 20 Mar 2009 07:01:13 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: mike.net.mo-at-gmail.com (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Change the contrast between the outer and inner of the
6, 12 -- diffraction circle to a linear value
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Fri, 20 Mar 2009 07:43:39 -0500
Subject: [Microscopy] Re: TEM-reply to Invitation to a new EM community site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One of my sons favourite Back Street Boys songs has the line "I don't
care just who you are, What you've done...."

Unfortunately that does not apply in science. When you do a bad job of
plagiarizing another site, and admit it, and say that you want to remain
anonymous for any reason you destroy any credibility.

Paul Hazelton, not anonymous

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Fri Mar 20 07:43:39 2009
6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KChdjU015704
6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 07:43:39 -0500
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 -- (authenticated bits=0)
6, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2KChbUv012255;
6, 20 -- Fri, 20 Mar 2009 07:43:37 -0500 (CDT)
6, 20 -- Message-ID: {49C38F7B.60602-at-umanitoba.ca}
6, 20 -- Date: Fri, 20 Mar 2009 07:43:39 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: samwarren-at-emfocal.com
6, 20 -- Subject: Re: [Microscopy] TEM-reply to Invitation to a new EM community site
6, 20 -- References: {200903200919.n2K9Jb1u004043-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200903200919.n2K9Jb1u004043-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: petrov-at-mrl.uiuc.edu
Date: Fri, 20 Mar 2009 11:32:12 -0500
Subject: [Microscopy] 3rd Materials Characterization Workshop, Center for Microanalysis of Materials, Univeristy of Illinois

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,



This is to inform you and invite any interested participants to the 3rd Advanced Materials Characterization Workshop 2009, organized by the Center for Microanalysis of Materials, Frederick Seitz Materials Research Laboratory, Univeristy of Illinois.

Dates: June 3 and 4, 2009

More information at: http://cmm.mrl.uiuc.edu/workshop2009/ {http://cmm.mrl.uiuc.edu/workshop2009/}



Kind regards



Ivan Petrov

217 333 8396



p.s. The workshop will provide a condensed overview of the most important analytical techniques with strong focus in practical applications and problem solving strategies. The lectures will cover mainstream techniques such as:



atomic force microscopy (AFM)

x-ray diffraction, reflectivity and fluorescence (XRD, XRR and XRF)

scanning electron microscopy (SEM)

focused ion beam (FIB)

Auger electron spectroscopy (AES)

x-ray photoelectron spectroscopy (XPS)

transmission and scanning transmission electron microscopy (TEM, STEM)

secondary ion mass spectrometry (SIMS)

Rutherford backscattering (RBS)

optical spectroscopy (Raman, Photoluminescence, FTIR, ellipsometry), etc.

The lectures will be given by FS-MRL facility scientists with 10+ years of hands-on experience in each particular technique. The workshop will cover:



The fundamentals of each analytical technique.

Comparative review of the instrumentation options with emphasis on differences in resolution, sensitivity, sample requirements.

Data acquisition strategies and data interpretation methods.

Expert tips on how to avoid measurement artifacts.

Critical review of strengths and weaknesses of each technique: how to combine techniques to extract the best possible complementary information.

Detailed discussion of practical examples including industrial applications in nanotechnology, microelectronics, thin films, coatings, bioengineering, mineralogy, medical and pharmaceutical research.

Instrument vendors and industrial scientists will be present during the workshop to discuss new instrumentation, applications and technology. Laboratory tours and demonstration of the main instruments available at the CMM will be given during the workshop.



Registration is required.

Register now - space is limited.



Join the sponsors:

Asylum Research

AVS - University of Illinois at Urbana-Champaign Student Chapter

Bruker AXS

Chemplex

FEI

Gatan

Materials Data

PANalytical

Physical Electronics (PHI)



==============================Original Headers==============================
48, 20 -- From petrov-at-mrl.uiuc.edu Fri Mar 20 11:32:12 2009
48, 20 -- Received: from mrlnt6.mrl.uiuc.edu (mrlnt6.mrl.uiuc.edu [130.126.101.9])
48, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KGWBLq004314
48, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 11:32:12 -0500
48, 20 -- Content-Class: urn:content-classes:message
48, 20 -- MIME-Version: 1.0
48, 20 -- Content-Type: text/plain;
48, 20 -- charset="utf-8"
48, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6619.12
48, 20 -- Subject: 3rd Materials Characterization Workshop, Center for Microanalysis of Materials, Univeristy of Illinois
48, 20 -- Date: Fri, 20 Mar 2009 11:32:09 -0500
48, 20 -- Message-ID: {9EADC1E53F9C70479BF6559370369114011E7034-at-mrlnt6.mrl.uiuc.edu}
48, 20 -- X-MS-Has-Attach:
48, 20 -- X-MS-TNEF-Correlator:
48, 20 -- Thread-Topic: 3rd Materials Characterization Workshop, Center for Microanalysis of Materials, Univeristy of Illinois
48, 20 -- Thread-Index: AcmpeWpl2aNdy6v2QymB4V99Z1/x8Q==
48, 20 -- From: "Ivan Petrov" {petrov-at-mrl.uiuc.edu}
48, 20 -- To: {Microscopy-at-microscopy.com}
48, 20 -- Content-Transfer-Encoding: 8bit
48, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2KGWBLq004314
==============================End of - Headers==============================




From: crispin.hetherington-at-materials.ox.ac.uk
Date: Fri, 20 Mar 2009 11:36:13 -0500
Subject: [Microscopy] Job opening, EM Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The EM Facility in the Department of Materials, University of Oxford,
has an opening for an Electron Microscope Technician.
The job involves the maintenance and repair of the instruments, the
basic instruction of users in the operation of the instruments and the
day to day running of the facility.
The closing date for applications is Tuesday 14 April 2009.
For details please see http://www.materials.ox.ac.uk/vacancies/

Thanks!
Dr Crispin Hetherington
Department of Materials
University of Oxford, UK
www-em.materials.ox.ac.uk





==============================Original Headers==============================
6, 26 -- From crispin.hetherington-at-materials.ox.ac.uk Fri Mar 20 11:36:12 2009
6, 26 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161])
6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KGaBYF007464
6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 11:36:12 -0500
6, 26 -- Received: from smtp0.mail.ox.ac.uk ([129.67.1.205])
6, 26 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.69)
6, 26 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} )
6, 26 -- id 1LkhhZ-0005Cm-36
6, 26 -- for Microscopy-at-microscopy.com; Fri, 20 Mar 2009 16:36:09 +0000
6, 26 -- Received: from crispin-macmini.materials.ox.ac.uk ([129.67.84.177])
6, 26 -- by smtp0.mail.ox.ac.uk with esmtpsa (TLSv1:AES128-SHA:128)
6, 26 -- (Exim 4.69)
6, 26 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} )
6, 26 -- id 1LkhhZ-0003pQ-2M
6, 26 -- for Microscopy-at-microscopy.com; Fri, 20 Mar 2009 16:36:09 +0000
6, 26 -- Message-Id: {8649EDB7-9084-4191-803E-16FC41BE4A2B-at-materials.ox.ac.uk}
6, 26 -- From: Crispin Hetherington {crispin.hetherington-at-materials.ox.ac.uk}
6, 26 -- To: Microscopy-at-microscopy.com
6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 26 -- Content-Transfer-Encoding: 7bit
6, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 26 -- Subject: Job opening, EM Technician
6, 26 -- Date: Fri, 20 Mar 2009 16:36:08 +0000
6, 26 -- References: {B7BF4B3D-E6EB-492A-8ED5-6F355CF18769-at-materials.ox.ac.uk}
6, 26 -- X-Mailer: Apple Mail (2.930.3)
6, 26 -- X-Oxford-Username: crispin
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Sun, 22 Mar 2009 17:13:25 -0500
Subject: [Microscopy] RE: TEM length calibration standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 20, 2009, at 5:01 AM, mike.net.mo-at-gmail.com wrote:

} Title-Subject: [Filtered] Change the contrast between the outer and
} inner of the diffraction circle to a linear value
}
} Question: In the experiment we imaged a silica
} bead(2um) , and we gained the image of a silica bead
} which was a diffraction ring, and the brightness of the diffraction
} ring changed with the distance. (darker in the circle and
} lower outside the circle). Now we want to change the contrast
} between the outer and inner of the diffraction circle to a linear
} value so that I can know the positon of the bead, I don't know which
} lens or optical parts can be used to achieve this function.
}
Dear Mike,
Since no one else has tackled this, I'll give it a try. You don't
say whether you are doing EM or LM, but the answer is much the same.
In either case, you have phase contrast, which depends on the finite
wavelength of the particles used for imaging. In the case of TEM, the
width of the fringes depends on the amount of defocus, so if you take
a series of images with varying focus values--a through-focus series--
you can make the width of the fringes get smaller, then larger, and
you will get a sign reversal when going through focus. What you
describe above would be an overfocus fringe in TEM, if you are
describing a CCD image. (It would be underfocus, if you are
describing a photographic negative.) In the LM, it all depends on the
imaging conditions. For some techniques, a phase plate is introduced
into the illumination pathway, so in that case, the width of the
fringes can be changed by adjusting the position of the phase plate.
Warning: I have not done any LM, except on rare occasion to see if
cells I was culturing looked like they were growing properly, so I'm
just guessing on what can be adjusted or how to do it. If you
describe what you are doing more completely, perhaps a real expert
would be able to give you better advice.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Fri Mar 20 18:40:20 2009
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KNeJWI011759
4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 18:40:19 -0500
4, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
4, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id EB44D2E50716;
4, 22 -- Fri, 20 Mar 2009 16:40:17 -0700 (PDT)
4, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
4, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
4, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 691DB2E503DB;
4, 22 -- Fri, 20 Mar 2009 16:40:16 -0700 (PDT)
4, 22 -- Message-Id: {9899E8A7-9440-45A3-AAAD-4F584FDBB0A1-at-caltech.edu}
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- To: mike.net.mo-at-gmail.com, microscopy-at-microscopy.com
4, 22 -- In-Reply-To: {200903201201.n2KC1RkL001032-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
4, 22 -- Subject: Re: [Microscopy] viaWWW: Change the contrast between the outer and inner of the
4, 22 -- Date: Fri, 20 Mar 2009 16:40:16 -0700
4, 22 -- References: {200903201201.n2KC1RkL001032-at-ns.microscopy.com}
4, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================

From serenajones300-at-netscape.net Sat Mar 21 09:54:15 2009
Return-Path: {serenajones300-at-netscape.net}
Received: from google.com (cpc4-brig10-0-0-cust702.brig.cable.ntl.com [81.108.230.191])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2LEsEI6014487
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 21 Mar 2009 09:54:15 -0500
Received: from [102.149.75.174] (HELO google.com)
by tall-rap.co.uk; Sat, 21 Mar 2009 14:54:13 +0000
Message-ID: {00000001EBD322F957528816}
Reply-To: Dinah Elless {1298gretzinger.gunnar-at-gmail.com}

Dear All

My laboratory is participating in an electron microscopy length calibration
exercise to understand the precision, accuracy and long-term reliability of
distance measurements made with TEM.

Q1. I am aware that NIST have a standard procedure for calibrating SEMs and
carrying out measurements, but am I correct is saying there is no equivalent
for TEM - either from NIST or any other agency?

Q2. Am I also correct in believing there are no accredited distance
calibration standards for TEM ie a certified standard produced by NIST or
similar? I am aware of MagiCal, diffraction gratings and crystal lattices as
references, but none of these are certified standards - note standards and
references are often confused, but are not the same thing.

Q3. If anyone has carried out a similar exercise to this and has published
the results, I'd be interested in any references.

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


==============================Original Headers==============================
12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009
12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au [129.78.220.1])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2MMDOvC007699
12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24 -0500
12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1])
12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST)
12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19])
12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST)
12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ;
12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000
12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618
12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100
12, 24 -- Subject: RE: TEM length calibration standards
12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au}
12, 24 -- Thread-Topic: TEM length calibration standards
12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg==
12, 24 -- Mime-version: 1.0
12, 24 -- Content-type: text/plain;
12, 24 -- charset="US-ASCII"
12, 24 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: samwarren-at-emfocal.com
Date: Sun, 22 Mar 2009 21:22:50 -0500
Subject: [Microscopy] Re: TEM-reply to Invitation to a new EM community site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just want to have a frank and honest talk with you. It seems that my
decision of keeping anonymous was a wrong decision at the start. I will
put my name on it later (after several months testing). I still think this
is the initial testing stage (usage, politics, user feed back, etc.)

I don't know what I have done wrong, besides being anonymous, to cause
some of you "hate" this site. I have all the good intention to start this
site. I am a researcher just like many of you here. I have been working in
the field of TEM for almost 20 yearsC and loving itB

No rush on using the site. Just want you guys know it is there.
I am really hopping some of you may end up helping me moderate the forum,
wiki books, faqsC image galleries on the site.
I used the term gadministratorsh on the site, by that I meant some of
you in the future. I can have some people helping me administrating the
site.
I will simplify the gterms of usehB I did not start this site as a
personB Mainly because to separate myself from any possible legal issue
with the siteB I cannot start the site without some legal claimsB Some
of the terms may sound like offensiveB I am sorry about thatBAgainC no
experience writing that stuffC and I donet want to miss some critical
points so that someone can sue meB
AgainC I broadcast the site here for announcementC and also soliciting
your inputB

RegardsC
Sam

} You "site" is doomed to fail, amongst the users
} of this MSA listserver. If only for your poor
} communication skills.
}
} sorry,
}
} Jim Quinn
}
}
}
}
}
} } From mail-at-ns.microscopy.com Fri Mar 20 05:17:21 2009
} } Return-Path: {mail-at-ns.microscopy.com}
} } Received: from ns.microscopy.com (microscopy.com [206.69.208.10])
} } by www.matscieng.sunysb.edu (8.11.6/8.11.6) with ESMTP id n2KAHJM28179
} } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 20 Mar 2009 05:17:20 -0500
} } Received: from ns.microscopy.com (localhost.localdomain [127.0.0.1])
} } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2K9HvRv002063
} } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 20 Mar 2009 04:17:57 -0500
} } Received: (from mail-at-localhost)
} } by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id
} n2K9Hvb2002060;
} } Fri, 20 Mar 2009 04:17:57 -0500
} } Date: Fri, 20 Mar 2009 04:17:57 -0500
} } Message-Id: {200903200917.n2K9Hvb2002060-at-ns.microscopy.com}
} } To: jquinn-at-www.matscieng.sunysb.edu
} } From: samwarren-at-emfocal.com
} } Reply-to: samwarren-at-emfocal.com
} } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} } Subject: [Microscopy] TEM-reply to Invitation to a new EM community
} site
} } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } X-lewp: MicroscopyListSpam NAGS
} } Status: R
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } It seems some people concerned about the legal terms of the site. To
} tell
} } you the truth, I am total new in starting a community site. I found
} that
} } term of use which is free to use. So I modified it a little bit and
} used
} } it. I definitely will write one of my own. All want to say about the
} terms
} } of use are: (1) Do not abuse the site; (2) I am not responsible on what
} } you post; (3) Copyright belongs to the individual users; (4) I hold the
} } right to modify/delete some contents which may be improper to keep.
} } I may need to add some more. All mostly to cover my ass if there are
} some
} } legal issues come up.
} } Yes, the terms of use was mostly copied from Harvard website. I just
} found
} } "Harvard" name was still left in the terms of use. i just removed it.
} It
} } is not related to Harvard at all. I just borrowed their writing.
} } Believe, my primary reason for setting this up is to serve the
} community.
} } I have no problem to show my identity. But I am worried showing my real
} } name may turn someone away from using it. I myself would not like to
} post
} } much on some individual research group site. I keep anonymous just to
} make
} } you think I am nobody. Don't care about me. This site is for everyone
} to
} } use. I believe a site making no money will not be long-lived. I want
} the
} } information posted there to stay for hundreds of years.
} } Think about it, what harm can it do to me if I show myself up? None. It
} is
} } not a site against any laws. Why would someone want to shut me up? I
} think
} } this site is straight forward enough. It is for everyone to use. Any
} } individual research group site will not attract world-wide
} participation.
} } Do you believe a post-doc or a graduate student can keep the site alive
} } long? Do you all want to post on FEI site, JEOL site? The only way to
} make
} } the site long live is to commercialize it and make it a dedicated
} } community server.
} } I spent most of my recent time on building the site, not on policies. I
} } welcome you comments and wish you can help me out to straighten this
} site
} } out. I think the functionality of the site is ok. But I am really not
} good
} } on setting up policies. But I do need legal cover for myself.
} } Again, I sepnt some time on the site structure, but not on the content.
} I
} } intent to post some of my writting there. I may single-handedly write
} some
} } TEM, EELS, STEM, FIB, SEM, EDS tutorials. This site may end up mostly
} just
} } like that. But I encourage your contribution. I have seen many valuable
} } information scattered around list servers, and emails. I just wish they
} } can accumulate at a dedicated place and useful for a long time.
} } I maybe wrong about keeping the site owner anonymous. It can change if
} } most of you demand it.
} } At my work, I helped many people on TEM and other techniques. I felt
} some
} } of my answers may be written as a FAQ for my colleagues to see. Then I
} } came up an idea about building a TEM-FAQ site. I started searching the
} } ways to build the site and I found out I can do a lot more than a FAQ.
} And
} } we need more than a FAQ-site. So, after several months, I came up with
} } this:
} } http://www.emfocal.com
} } This site is in fact a personal site. But if this site can gain some
} money
} } it can live longer and not limited by me as a single person. You see
} many
} } forum, blog, community sites around. My intention is the same as most
} of
} } them.
} } Enough said for now. It will be too long for people to read.
} }
} } Thank you for you attention.
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 4, 31 -- From samwarren-at-emfocal.com Fri Mar 20 04:16:59 2009
} } 4, 31 -- Received: from outbound-mail-352.bluehost.com
} (outbound-mail-352.bluehost.com [66.147.249.13])
} } 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n2K9Gwka000946
} } 4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Mar 2009 04:16:58
} -0500
} } 4, 31 -- Received: (qmail 11610 invoked by uid 0); 20 Mar 2009 09:12:13
} -0000
} } 4, 31 -- Received: from unknown (HELO host361.hostmonster.com)
} (66.147.240.161)
} } 4, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 20
} Mar 2009 09:12:13 -0000
} } 4, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default;
} d=emfocal.com;
} } 4, 31 --
} h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
} } 4, 31 --
} b=XNU9zD7exEBRUvI//bp7eRTNLZVIEWnKeStnLA9+GjB3MG4U/BaUv3xAvXIQ9468Xypa9rCpHaEmQGoIakW/RjLZ4wwnzEmv7IwkTZ78A9+WXBqQPWp6W9uOETm+YLcL;
} } 4, 31 -- Received: from localhost ([127.0.0.1]
} helo=host361.hostmonster.com)
} } 4, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
} } 4, 31 -- (envelope-from {samwarren-at-emfocal.com} )
} } 4, 31 -- id 1LkaqX-0005VN-8B
} } 4, 31 -- for Microscopy-at-Microscopy.Com; Fri, 20 Mar 2009 03:16:58
} -0600
} } 4, 31 -- Received: from 124.192.231.2 ([124.192.231.2])
} } 4, 31 -- (SquirrelMail authenticated user
} samwarren-at-emfocal.com)
} } 4, 31 -- by host361.hostmonster.com with HTTP;
} } 4, 31 -- Fri, 20 Mar 2009 03:16:57 -0600 (MDT)
} } 4, 31 -- Message-ID:
} {1675.124.192.231.2.1237540617.squirrel-at-host361.hostmonster.com}
} } 4, 31 -- Date: Fri, 20 Mar 2009 03:16:57 -0600 (MDT)
} } 4, 31 -- Subject: TEM-reply to Invitation to a new EM community site
} } 4, 31 -- From: samwarren-at-emfocal.com
} } 4, 31 -- To: Microscopy-at-Microscopy.Com
} } 4, 31 -- User-Agent: SquirrelMail/1.4.13
} } 4, 31 -- MIME-Version: 1.0
} } 4, 31 -- Content-Type: text/plain;charset=iso-8859-1
} } 4, 31 -- Content-Transfer-Encoding: 8bit
} } 4, 31 -- X-Priority: 3 (Normal)
} } 4, 31 -- Importance: Normal
} } 4, 31 -- X-Identified-User:
} {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program
} running on server}
} } ==============================End of -
} Headers==============================
} }
}



==============================Original Headers==============================
7, 33 -- From samwarren-at-emfocal.com Sun Mar 22 21:22:49 2009
7, 33 -- Received: from outbound-mail-345.bluehost.com (outbound-mail-345.bluehost.com [66.147.249.6])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2N2Mlqm025968
7, 33 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Mar 2009 21:22:48 -0500
7, 33 -- Received: (qmail 12896 invoked by uid 0); 23 Mar 2009 02:17:54 -0000
7, 33 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161)
7, 33 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 23 Mar 2009 02:17:54 -0000
7, 33 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com;
7, 33 -- h=Message-ID:In-Reply-To:References:Date:Subject:From:To:Cc:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
7, 33 -- b=tSH6R0ikF86A8PYCAkH7YcI1F9IHf16WuhpC962tjU/+f+cjvNCgutvLZD/gkyd9/VjSvZZk2BGLVSaSR1V89JcZke41Tv14BN5TcfFlIFo/31AExQhXC+Hk9DO/4G9N;
7, 33 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com)
7, 33 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
7, 33 -- (envelope-from {samwarren-at-emfocal.com} )
7, 33 -- id 1LlZoL-0006Wy-0m; Sun, 22 Mar 2009 20:22:45 -0600
7, 33 -- Received: from 124.192.231.7 ([124.192.231.7])
7, 33 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
7, 33 -- by host361.hostmonster.com with HTTP;
7, 33 -- Sun, 22 Mar 2009 20:22:45 -0600 (MDT)
7, 33 -- Message-ID: {1544.124.192.231.7.1237774965.squirrel-at-host361.hostmonster.com}
7, 33 -- In-Reply-To: {200903201720.n2KHKhx28887-at-www.matscieng.sunysb.edu}
7, 33 -- References: {200903201720.n2KHKhx28887-at-www.matscieng.sunysb.edu}
7, 33 -- Date: Sun, 22 Mar 2009 20:22:45 -0600 (MDT)
7, 33 -- Subject: Re: [Microscopy] TEM-reply to Invitation to a new EM community site
7, 33 -- From: samwarren-at-emfocal.com
7, 33 -- To: "Jim Quinn" {jquinn-at-www.matscieng.sunysb.edu}
7, 33 -- Cc: Microscopy-at-Microscopy.Com
7, 33 -- User-Agent: SquirrelMail/1.4.13
7, 33 -- MIME-Version: 1.0
7, 33 -- Content-Type: text/plain;charset=iso-8859-1
7, 33 -- Content-Transfer-Encoding: 8bit
7, 33 -- X-Priority: 3 (Normal)
7, 33 -- Importance: Normal
7, 33 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server}
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Sun, 22 Mar 2009 22:35:56 -0500
Subject: [Microscopy] Re: [a] RE: TEM length calibration standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We make several traceable and non-traceable calibration standards for SEM
and AFM, now including a 70-nm pitch grating. What procedure would one use
to make a TEM specimen from a grating? If the procedure is to cast and then
peel a polymer replica, can this be done without significant distortion of
the length?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================
----- Original Message -----
From: david.mitchell-at-emu.usyd.edu.au
To: donc-at-asmicro.com
Sent: Sunday, March 22, 2009 6:18 PM
Subject: [a] [Microscopy] RE: TEM length calibration standards





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Dear All

My laboratory is participating in an electron microscopy length
calibration
exercise to understand the precision, accuracy and long-term reliability
of
distance measurements made with TEM.

Q1. I am aware that NIST have a standard procedure for calibrating SEMs
and
carrying out measurements, but am I correct is saying there is no
equivalent
for TEM - either from NIST or any other agency?

Q2. Am I also correct in believing there are no accredited distance
calibration standards for TEM ie a certified standard produced by NIST or
similar? I am aware of MagiCal, diffraction gratings and crystal lattices
as
references, but none of these are certified standards - note standards and
references are often confused, but are not the same thing.

Q3. If anyone has carried out a similar exercise to this and has published
the results, I'd be interested in any references.

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


==============================Original
Headers==============================
12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009
12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au
[129.78.220.1])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2MMDOvC007699
12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24 -0500
12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1])
12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100
(EST)
12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au
[172.17.185.19])
12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100
(EST)
12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by
MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server
www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server
HTTP-DAV ;
12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000
12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618
12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100
12, 24 -- Subject: RE: TEM length calibration standards
12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au}
12, 24 -- Thread-Topic: TEM length calibration standards
12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg==
12, 24 -- Mime-version: 1.0
12, 24 -- Content-type: text/plain;
12, 24 -- charset="US-ASCII"
12, 24 -- Content-transfer-encoding: 7bit
==============================End of -
Headers==============================


==============================Original Headers==============================
21, 26 -- From donc-at-asmicro.com Sun Mar 22 22:35:56 2009
21, 26 -- Received: from smtp121.sbc.mail.re3.yahoo.com (smtp121.sbc.mail.re3.yahoo.com [66.196.96.94])
21, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2N3ZtRJ008785
21, 26 -- for {microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 22:35:55 -0500
21, 26 -- Received: (qmail 69341 invoked from network); 23 Mar 2009 03:35:53 -0000
21, 26 -- Received: from unknown (HELO asm15) (donc-at-98.226.80.44 with login)
21, 26 -- by smtp121.sbc.mail.re3.yahoo.com with SMTP; 23 Mar 2009 03:35:53 -0000
21, 26 -- X-YMail-OSG: P1j19lEVM1lKYCfXkN6Y_ykVgUS2Iu6Qx8P.hI0133lfsxxVHmvoopNJeJjCKNGT9v1uR0jXh1Jx4KK3NehHOM0tMqR0MSVnFNsbimK0qNI7ajbGeGgszeqcVw4RZQQapezMVR0qThFjZuvixm4H1OQhJh.CBVKImezStfkW4Jj5OQiO2scIg2ru1MU1XqxJ83is9B8FR3D4UCzIsNX0BHISrfIHpeU-
21, 26 -- X-Yahoo-Newman-Property: ymail-3
21, 26 -- Message-ID: {88BC6672C7E5479995672EAE7DF80ACE-at-asm15}
21, 26 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
21, 26 -- To: {david.mitchell-at-emu.usyd.edu.au}
21, 26 -- Cc: "Microscopy List" {microscopy-at-microscopy.com}
21, 26 -- References: {200903222218.n2MMIw9P012191-at-ns.microscopy.com}
21, 26 -- Subject: Re: [a] [Microscopy] RE: TEM length calibration standards
21, 26 -- Date: Sun, 22 Mar 2009 23:12:46 -0400
21, 26 -- MIME-Version: 1.0
21, 26 -- Content-Type: text/plain;
21, 26 -- format=flowed;
21, 26 -- charset="iso-8859-1";
21, 26 -- reply-type=original
21, 26 -- Content-Transfer-Encoding: 7bit
21, 26 -- X-Priority: 3
21, 26 -- X-MSMail-Priority: Normal
21, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
21, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Mon, 23 Mar 2009 12:04:11 -0500
Subject: [Microscopy] Re: fine-grained coal sample prep for SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I independently replied to Sarah last week from home. I am just now
replying to the list as my web-based mail client won't let me send plain
text messages, so they get bounced.

I followed another's suggestion to dissolve iodoform in epoxy to boost
the average atomic number of the epoxy without interfering with
polymerization. It is a somewhat nasty material, so it needs to be
handled with care. However, it definitely did the job for me for my
research on minerals in coal in the 1980s and 1990s.

I am curious how the polyester resin would offer an improvement.

Most polymers are quite similar in atomic number. I tried many in my
research to find a contrasting medium. Something like carnauba wax or
polyethylene or polystyrene have a higher H:C ratio than coal and
without many other functional groups that serve to raise the average
atomic number. However, wax and polyethylene should be the extreme
cases, yet the contrast was not all that satisfactory. Besides, as
thermoplastics, the preparation of mounts was somewhat problematic.
Raising the atomic number by the addition of a heavy element seemed a
better alternative.

Warren S.

-----Original Message-----
X-from: dljones-at-bestweb.net [mailto:dljones-at-bestweb.net]
Sent: Thursday, March 19, 2009 9:31 PM
To: wesaia-at-iastate.edu

Sarah,

I don't know the answer to your particular problem but in other cases
that
sound similar to what you are describing, I have found using a polyester

mount material works.

You can try:

http://www.extec.com/mounting/cold-mounting-systems.php

Scroll down to the bottom of their list and find their polyester mount
material. Part 14675 and 14685 for the smaller size. I don't know of
another source but I'm sure they exist.

standard disclaimer: I have no interest in this company except as a
satisfied customer...

dj

On Thu, 19 Mar 2009, sappleby-at-mines.edu wrote:

}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Dear all,
} I'm trying to mount fine-grained coal particles (-125 um) for
EDS-based
} automated mineral analysis. In a first attempt I used epoxy resin
} (Struers EpoFix), but the BSE intensity of epoxy and coal are too
} similar, which means the system can't differentiate easily between the
} two. After doing some background reading I tried mounting the material
} in molten Carnauba wax. Problem is the polishing is difficult as the
wax
} is extremely soft and the system is taking forever to pump down (I'm
not
} convinced yet it'll ever reach the required vacuum). I'm sure there
must
} be working protocols out there. Does anyone have any recommendations
or
} ideas?
}
} Thanks,
} Sarah Appleby
}
} --
} *********************************
} Dr. Sarah Appleby
} Technical Scientist
} Advanced Mineralogy Research Center
} Colorado School of Mines
} 1310 Maple Street
} Golden, CO 80401, USA
}
} office: 303-384-2487
} fax: 303-384-2499
} email: sappleby-at-mines.edu
}
} *********************************


==============================Original Headers==============================
18, 36 -- From wesaia-at-iastate.edu Mon Mar 23 12:04:10 2009
18, 36 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14])
18, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NH4A1l011189
18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:10 -0500
18, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
18, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n2NH49Qo030374
18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:09 -0500
18, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
18, 36 -- id 5ed8_a02c75a4_17cc_11de_9e53_001372578af6;
18, 36 -- Mon, 23 Mar 2009 12:04:09 -0500
18, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
18, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n2NH44oc029812
18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:07 -0500
18, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
18, 36 -- Mon, 23 Mar 2009 11:51:25 -0500
18, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
18, 36 -- Content-class: urn:content-classes:message
18, 36 -- MIME-Version: 1.0
18, 36 -- Content-Type: text/plain;
18, 36 -- charset="us-ascii"
18, 36 -- Subject: RE: [Microscopy] Re: fine-grained coal sample prep for SEM?
18, 36 -- Date: Mon, 23 Mar 2009 11:51:41 -0500
18, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703B904E0-at-maire.eng.iastate.edu}
18, 36 -- In-Reply-To: {200903200230.n2K2UgUX011661-at-ns.microscopy.com}
18, 36 -- X-MS-Has-Attach:
18, 36 -- X-MS-TNEF-Correlator:
18, 36 -- Thread-Topic: [Microscopy] Re: fine-grained coal sample prep for SEM?
18, 36 -- thread-index: AcmpA99ULhFfW+21Q/GltFXfoxFoPwC0jofQ
18, 36 -- References: {200903200230.n2K2UgUX011661-at-ns.microscopy.com}
18, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
18, 36 -- To: {Microscopy-at-microscopy.com}
18, 36 -- X-OriginalArrivalTime: 23 Mar 2009 16:51:25.0520 (UTC) FILETIME=[9A807D00:01C9ABD7]
18, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.23.164917
18, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0'
18, 36 -- Content-Transfer-Encoding: 8bit
18, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2NH4A1l011189
==============================End of - Headers==============================




From: RTemkin-at-mtsinai.on.ca
Date: Mon, 23 Mar 2009 14:32:48 -0500
Subject: [Microscopy] Cryo immuno EM of culture inserts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I have a client who wants to do cryo immuno EM of cells grown on culture
insert membranes. I notice that the membranes can be of various materials
such as PTFE or polycarbonate. Does anyone have experience cryo sectioning
insert membranes and if so which type of membrane sections well?

Thank you in advance.

Bob Temkin
Advanced Bioimaging Centre
Mount Sinai Hospital
Pathology and Laboratory Medicine
Toronto

rtemkin-at-mtsinai.on.ca


==============================Original Headers==============================
6, 21 -- From RTemkin-at-mtsinai.on.ca Mon Mar 23 14:32:48 2009
6, 21 -- Received: from mshemr01.mtsinai.on.ca (mshemr01.mtsinai.on.ca [38.112.100.7])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NJWlot030109
6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 14:32:47 -0500
6, 21 -- Received: from [142.223.135.154] ([142.223.135.154]:48139 "EHLO
6, 21 -- mshmail3.mtsinai.on.ca") by mshemr01.mtsinai.on.ca with ESMTP
6, 21 -- id S6873964AbZCWTcr (ORCPT {rfc822;Microscopy-at-microscopy.com} );
6, 21 -- Mon, 23 Mar 2009 15:32:47 -0400
6, 21 -- Received: by MSHMAIL3 with Internet Mail Service (5.5.2657.72)
6, 21 -- id {HJ1H3NQS} ; Mon, 23 Mar 2009 15:32:47 -0400
6, 21 -- Message-ID: {462EBF7807AE844E9AE4E0E70AF52C221EDDDD-at-mshmail12}
6, 21 -- From: "Temkin, Robert" {RTemkin-at-mtsinai.on.ca}
6, 21 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
6, 21 -- Subject: Cryo immuno EM of culture inserts
6, 21 -- Date: Mon, 23 Mar 2009 15:32:45 -0400
6, 21 -- MIME-Version: 1.0
6, 21 -- X-Mailer: Internet Mail Service (5.5.2657.72)
6, 21 -- Content-Type: text/plain;
6, 21 -- charset="iso-8859-1"
6, 21 -- X-BCN: Meridius 1000 Version 3.4 on mshemr01.mtsinai.on.ca
6, 21 -- X-BCN-Sender: RTemkin-at-mtsinai.on.ca
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Mon, 23 Mar 2009 15:13:28 -0500
Subject: [Microscopy] RE: TEM length calibration standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

David,
Since the Mag-i-cal sample traces all of the different lengths scales back
to the d-spacings of the silicon substrate, it does not need to be traced
back to NIST. If you buy a Mag-i-cal sample, there is included a letter
copied from NIST to the developer of the sample, John McCaffrey, to that
effect. I worked in a laboratory where we had to have traceable standards
for QS-9000 certification. That is similar to ISO quality programs but for
the automotive industry. The Mag-i-cal sample was accepted by the auditors
because of that letter and the fact that the lengths scales can be traced
back to the d-spacings of silicon.

The nice thing about using the Mag-i-cal sample for magnification
calibration was that it could be used over the entire range of
magnifications. That is not true for the other "standards" that are
available, such as the lattice spacings of certain crystals, diffraction
gratings, and polystyrene spheres. In my use of those, they were not
consistent over the magnification ranges where two could be used. The
Mag-i-cal sample does not have that problem. The other thing that is great
about the Mag-i-cal sample is that it is very difficult to damage it with a
beam.

Disclaimer: SBT sells the Mag-i-cal sample.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: david.mitchell-at-emu.usyd.edu.au [mailto:david.mitchell-at-emu.usyd.edu.au]

Sent: Sunday, March 22, 2009 3:20 PM
To: Walck-at-SouthBayTech.com

Dear All

My laboratory is participating in an electron microscopy length calibration
exercise to understand the precision, accuracy and long-term reliability of
distance measurements made with TEM.

Q1. I am aware that NIST have a standard procedure for calibrating SEMs and
carrying out measurements, but am I correct is saying there is no equivalent
for TEM - either from NIST or any other agency?

Q2. Am I also correct in believing there are no accredited distance
calibration standards for TEM ie a certified standard produced by NIST or
similar? I am aware of MagiCal, diffraction gratings and crystal lattices as
references, but none of these are certified standards - note standards and
references are often confused, but are not the same thing.

Q3. If anyone has carried out a similar exercise to this and has published
the results, I'd be interested in any references.

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


==============================Original Headers==============================
12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009
12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au
[129.78.220.1])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2MMDOvC007699
12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24
-0500
12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1])
12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22
+1100 (EST)
12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au
[172.17.185.19])
12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5
12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22
+1100 (EST)
12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by
MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server
www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server
HTTP-DAV ;
12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000
12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618
12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100
12, 24 -- Subject: RE: TEM length calibration standards
12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au}
12, 24 -- Thread-Topic: TEM length calibration standards
12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg==
12, 24 -- Mime-version: 1.0
12, 24 -- Content-type: text/plain;
12, 24 -- charset="US-ASCII"
12, 24 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================

Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
5:29 PM


==============================Original Headers==============================
24, 23 -- From walck-at-southbaytech.com Mon Mar 23 15:13:28 2009
24, 23 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82])
24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NKDSfr013059
24, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 15:13:28 -0500
24, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201])
24, 23 -- (authenticated bits=0)
24, 23 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n2NKDJX3000569;
24, 23 -- Mon, 23 Mar 2009 15:13:22 -0500
24, 23 -- From: "Scott Walck" {walck-at-southbaytech.com}
24, 23 -- To: {Microscopy-at-microscopy.com}
24, 23 -- Cc: {david.mitchell-at-emu.usyd.edu.au}
24, 23 -- References: {200903222219.n2MMJcsw012677-at-ns.microscopy.com}
24, 23 -- In-Reply-To: {200903222219.n2MMJcsw012677-at-ns.microscopy.com}
24, 23 -- Subject: RE: [Microscopy] RE: TEM length calibration standards
24, 23 -- Date: Mon, 23 Mar 2009 13:13:45 -0700
24, 23 -- Message-ID: {008001c9abf3$e09df260$a1d9d720$-at-com}
24, 23 -- MIME-Version: 1.0
24, 23 -- Content-Type: text/plain;
24, 23 -- charset="us-ascii"
24, 23 -- Content-Transfer-Encoding: 7bit
24, 23 -- X-Mailer: Microsoft Office Outlook 12.0
24, 23 -- Thread-Index: AcmrPEyzS+tFgNXTTuST+yUd2kc8FwAtbEVQ
24, 23 -- Content-Language: en-us
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Tue, 24 Mar 2009 00:52:27 -0500
Subject: [Microscopy] Staining for glycogen in resin-embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We have a researcher who is studying the effects of a particular drug on rat
liver, heart and spinal cord.
Tissue for EM was processed with osmium tetroxide, en-bloc stained with
uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy
resin. Thin sections were stained with lead citrate.
By EM we found increased amounts of glycogen in one of the liver samples.
The researcher asked "How do you know it's glycogen".
I said "It just is".
He is worried that his supervisor won't accept that it's glycogen just
because we said so. He wants to prove it.

So my question is...

Is there a stain for epoxy resin sections that will stain specifically for
glycogen?
Will periodic acid Schiff (PAS) work on resin-embedded sections?

Regards,

John Brealey


Supervisor - Electron Microscope Unit



E john.brealey-at-imvs.sa.gov.au

T 8222 6612

F 8222 6425

www.sapathology.sa.gov.au



SA Pathology (Queen Elizabeth Hospital)



Electron Microscope Unit, Surgical Pathology

SA Pathology

Queen Elizabeth Hospital

Woodville, 5011

AUSTRALIA



Quality Pathology supporting Training and Research



==============================Original Headers==============================
25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009
25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161
25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500
25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000";
25, 27 -- d="scan'208";a="16015375"
25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030
25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030
25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for
25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST)
25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
25, 27 -- Subject: Staining for glycogen in resin-embedded tissue
25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030
25, 27 -- Organization: IMVS
25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i}
25, 27 -- MIME-Version: 1.0
25, 27 -- Content-Type: text/plain; charset="us-ascii"
25, 27 -- Content-Transfer-Encoding: 7bit
25, 27 -- X-Mailer: Microsoft Office Outlook 11
25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ==
25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Tue, 24 Mar 2009 03:35:23 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

In spite of beeing interesting by the concept of the ESEM since long
ago, I had until now nor much time neither the real need to work on one.
So I've a couple of questions about it.

1- First, most pictures done by ESEM in wet mode I've seen from
collegues, either biologists or from material science, are done at high
energy, 20-30 kV. It seems that the low energy "culture" is a bit
ignored. (On the other hand, I agree that I'm perheps a bit addict to
the low kV range !) But apart the subjecives aspects like "traditions",
lab "culture" or the need of EDS, is there a more objective and
technical reason for such choice ? Do the wet conditions limit so
drastically the performences of the ESEM at low energy (lower then 5 keV
f.ex.) ?

2- I a paper published in M&M 6-2000, Danilatos describes a very
interesting evolution of the PLA (in particular for people interested in
vaccum technology, like me...), called Reverse Flow Pressure Limiting
Apperture. An annular gas jet flows from an intermediate stage at higher
pressure into the specimen chamber, generating a pumping effect in the
central part of the PLA and allowding to work with a greater
differential pressure between the chamber and the column.
I asked me if such a device has been soon integrated in commercial
VP-SEMs, from FEI or other manufactuers. Danilatos mention too a
cooperation with Zeiss on his website, but I never heard about a
developpment like that by Zeiss.

Thanks for comments

Jacques

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 03:35:23 2009
9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.157])
9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O8ZMax011825
9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 03:35:23 -0500
9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 09:35:21 +0100 (CET)
9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 29 -- (No client certificate requested)
9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 5F52D3EC002
9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar 2009 09:34:14 +0100 (CET)
9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100
9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
9, 29 -- MIME-Version: 1.0
9, 29 -- To: microscopy-at-microscopy.com
9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 29 -- Content-Transfer-Encoding: 8bit
9, 29 -- X-IPCMS-MailScanner: Found to be clean
9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 29 -- Subject: Quest. about the ESEM
9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET)
9, 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 2009 on mr7.u-strasbg.fr
9, 29 -- X-Virus-Status: Clean
9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
9, 29 -- autolearn=disabled version=3.2.5
9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr7.u-strasbg.fr
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Tue, 24 Mar 2009 03:41:10 -0500
Subject: [Microscopy] Re: Staining for glycogen in resin-embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
there is a Thiery's silverproteinate method for glycogen staining in ultrathin
sections on EM grid:
---------------------------
Thiery, J.-P. 1967. Mise en evidence des polysaccharides sur coupes fines en
microscopie electronique. J. Microsc. 6:987–1018.
--------------------------

We have use it with success to proof glycogen-like polysaccharides in
Streptomyces long time ago, (1996).

With regards Oldrich

--
Oldrich Benada
Institute of Microbiology, Acad. Sci. CR, v.v.i.
Videvská 1083
142 20 Prague 4 - Krc
Czech Republic

On Tuesday 24 March 2009 06:55:32 am john.brealey-at-imvs.sa.gov.au wrote:
} ---------------------------------------------------------------------------
} - The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------
} -
}
} Hi,
}
} We have a researcher who is studying the effects of a particular drug on
} rat liver, heart and spinal cord.
} Tissue for EM was processed with osmium tetroxide, en-bloc stained with
} uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy
} resin. Thin sections were stained with lead citrate.
} By EM we found increased amounts of glycogen in one of the liver samples.
} The researcher asked "How do you know it's glycogen".
} I said "It just is".
} He is worried that his supervisor won't accept that it's glycogen just
} because we said so. He wants to prove it.
}
} So my question is...
}
} Is there a stain for epoxy resin sections that will stain specifically for
} glycogen?
} Will periodic acid Schiff (PAS) work on resin-embedded sections?
}
} Regards,
}
} John Brealey
}
}
} Supervisor - Electron Microscope Unit
}
}
}
} E john.brealey-at-imvs.sa.gov.au
}
} T 8222 6612
}
} F 8222 6425
}
} www.sapathology.sa.gov.au
}
}
}
} SA Pathology (Queen Elizabeth Hospital)
}
}
}
} Electron Microscope Unit, Surgical Pathology
}
} SA Pathology
}
} Queen Elizabeth Hospital
}
} Woodville, 5011
}
} AUSTRALIA
}
}
}
} Quality Pathology supporting Training and Research
}
}
}
} ==============================Original
} Headers============================== 25, 27 -- From
} john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 25, 27 -- Received:
} from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 25, 27 --
} by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2O5qP3Q011161 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009
} 00:52:26 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000";
} 25, 27 -- d="scan'208";a="16015375"
} 25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au)
} ([10.9.18.85]) 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5;
} 24 Mar 2009 16:22:24 +1030 25, 27 -- Received: from ablett.imvs.sa.gov.au
} (10.20.98.41) by
} 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP
} Server id 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030
} 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au
} [10.20.138.125]) by 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP
} id E9FE02EC078 for 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009
} 16:22:23 +1030 (CST) 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
} 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
} 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue
} 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030
} 25, 27 -- Organization: IMVS
} 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i}
} 25, 27 -- MIME-Version: 1.0
} 25, 27 -- Content-Type: text/plain; charset="us-ascii"
} 25, 27 -- Content-Transfer-Encoding: 7bit
} 25, 27 -- X-Mailer: Microsoft Office Outlook 11
} 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ==
} 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
} ==============================End of -
} Headers==============================





==============================Original Headers==============================
9, 26 -- From benada-at-biomed.cas.cz Tue Mar 24 03:41:10 2009
9, 26 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O8f9Cf021252
9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 03:41:09 -0500
9, 26 -- Received: from u117ob.mbu.cas.cz (u117ob.mbu.cas.cz [147.231.44.101])
9, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 26 -- (No client certificate requested)
9, 26 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id E47CF1A44571;
9, 26 -- Tue, 24 Mar 2009 09:41:08 +0100 (CET)
9, 26 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
9, 26 -- Reply-To: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz}
9, 26 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i.
9, 26 -- To: john.brealey-at-imvs.sa.gov.au
9, 26 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue
9, 26 -- Date: Tue, 24 Mar 2009 09:40:34 +0100
9, 26 -- User-Agent: KMail/1.9.9
9, 26 -- References: {200903240555.n2O5tWfm015327-at-ns.microscopy.com}
9, 26 -- In-Reply-To: {200903240555.n2O5tWfm015327-at-ns.microscopy.com}
9, 26 -- Cc: microscopy-at-microscopy.com
9, 26 -- MIME-Version: 1.0
9, 26 -- Content-Type: text/plain;
9, 26 -- charset="utf-8"
9, 26 -- Content-Disposition: inline
9, 26 -- Message-Id: {200903240940.34340.benada-at-biomed.cas.cz}
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2O8f9Cf021252
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Tue, 24 Mar 2009 04:38:39 -0500
Subject: [Microscopy] Re: Staining for glycogen in resin-embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,
Dear John,
hello, dear all,

it is interesting to learn about "researchers" doubting about "experience" of long-time serious electron microscopist that have learned their job.

One has to admit that there ARE or probably COULD be some problems in "demonstrating" or localizing (= precipitating / retaining) glycogen particles within cellular structures, depending on the processing, especially the first steps of correct or at least "promising" fixation.

So, as to my knowledge, there are legion of recipes, special fixation methods and some tricks one can use to unequivocally demonstrate glycogen (particles).
Nevertheless, judged only on morphological feature(s), sometimes it "might" be that ribosomal particles could be mistaken to be "perhaps" glycogen particles.

You should point your "researcher" to hundreds of papers (e.g.1965-1980&&) dealing with the specific localisation / demonstration of the stuff on ultrastructural grounds:

So here are my two Euro-cents:
Using general sources: like also e.g. GEYER, G.(ed.), Ultrahistochemistry, 1973
(knowing that perhaps better standard books [Hayat's, Bozzola, Maunsbach etc.] are out there in the wild....):

- Tissue fixed with (FA-)GA & OsO4 alone: without positive staining ('contrasting'): no glycogen demo possible
- Tissue fixed with (FA-)GA & OsO4, section staining with MJ Karnovsky's(1961)Pb-solution, or also KMnO4 (perhaps Mike Reedy will comment? on this because of unfavorable conditions if MNA is contained in the resin mixture) according to Lawn (1960),
- Even the good old double staining (UO2Ac-Pb-citrate [Reynolds as well as Venable&Coggeshall) after "good" fixation are sufficient to achieve excellent glycogen staining (if it has not leached out/turned over during e.g. delayed fixation or use of poor primary fixative ).
There (hereafter) also is to be mentioned the improvement in selective staining for glycosylated binding sites (i. e. glycogen, collagen, elastin) using the method of

Koert P. DINGEMANS and Marius A. van den BERGH WEERMAN ["Rapid Contrasting of Extracellular Elements in Thin Sections", Ultrastructural Pathology 14:519-527, 1990].
In their article one can learn a lot about staining properties and use of TANNIC Acid preincubation (prior to the classical "UO2-Pb-double staining") and the consequences in terms of increased e-density of sectioned substrate components.

I am not going into further detail for:
- Glycogen staining with "Best's Carmine" (e.g. according to Themann H, 1960 J. Ultrastruct. Res 4, pp.401-412),
- Glycogen staining using periodic acid incubation followed by staining with Reynolds Pb-citrate (according to PERRY MM, 1967)
- Glycogen staining enhanced by using K3Fe*III*(CN)6 and(=in) 1%OsO4 (De Bruyn WC, 1968),
- the PAT-Silver-proteinate Reaction (using Thiocarbohydrazide or Thiosemicarbazide) according to Thiery JP (1967),
- the PA-PFPH-reaction according to BRADBURY S & STOWARD PJ (Histochemie 11,pp 71-80, 1967) etc., etc.

Being quite sure that there are a lot of other special techniques additionally available (certainly other listers will comment on......)


Addressing the probable failing of glycogen staining after UO2 Acetate-En Bloc staining (as you informed us about the specimen processing conditions:

Citing from DARDICK, Irving and Ian ROOB's CD-ROM-Edition:

PRIMER of Diagnostic Electron Microscopy for Pathologists - in - training,
(Pathology Images Inc.,Ottawa, CANADA, (c) 2005 ISBN 0-9736518-0-6):

"Glycogen
Glycogen, lipid and mitochondria collectively are involved in energy metabolism, and glycogen and lipid are reserve materials in this context. Glycogen forms granules which show variations in staining characteristics but which are typically electron-dense. At low power, aggregates of glycogen granules may present as "lakes" of amorphous material and only high magnification reveals the distinctive particulate substructure. Glycogen is easily leached out of cytoplasm, especially by uranyl acetate when used as an en bloc stain, and results in what appear to be "clear" spaces. Even in this circumstance, however, the electron microscopic features are characteristic enough to indicate that glycogen was originally present. Glycogen is an important diagnostic marker for both pediatric and adult tumors.

Glycogen granules are present as two cytoplasmic forms:
the alpha-rosettes (typical of glycogen in liver cells) and the smaller monoparticulate beta-glycogen.

Legend Figure 1. A. (not included here): Cells from a rhabdomyoma in which large portions of the cytoplasm are occupied by what appear to be amorphous "lakes" of glycogen (G). X7,400. B. Cell from a small round-cell tumor showing focal areas of leached out glycogen (G) as a result of en bloc uranyl acetate staining. The homogeneous, rather glassy appearance is characteristic and there may be residual particulate glycogen at the margins of the glassy zones. X8,100.

Legend Figure 2. A. (not included here): Typical alpha-rosettes of glycogen in a hepatocyte filling the cytoplasm between mitochondria and a few segments of sER. X29,000. B. Monoparticulate beta-glycogen particles (20-30 nm across) filling the cytoplasmic spaces between mitochondria in a rhabdomyoblast. X84,000

Legend Figure 3. (not included here): Renal cell carcinoma with three adjacent tumor cells with accumulations of glycogen (G) in the cytoplasm. X9,200

Legend Figure 6. (not included here): Higher magnification of a Ewing's sarcoma tumor cell with an aggregate of the alpha-rosette form of glycogen (G) in the cytoplasm. X16,000.
&, &, & "


Best wishes and good luck,
Wolfgang Muss
EM-Lab, Inst. Pathology
SALK-PMU (Gen.Hospital, Private Paracelsus Medical University)
Salzburg, Austria




} -----Ursprüngliche Nachricht-----
} Von: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au]
} Gesendet: Dienstag, 24. März 2009 06:57
} An: Muß Wolfgang
} Betreff: [Microscopy] Staining for glycogen in resin-embedded tissue
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi,
}
} We have a researcher who is studying the effects of a
} particular drug on rat liver, heart and spinal cord.
}
} Tissue for EM was processed with osmium tetroxide, en-bloc
} stained with uranyl acetate, dehydrated with alcohols and
} embedded in Procure 812 epoxy resin.
} Thin sections were stained with lead citrate.
} By EM we found increased amounts of glycogen in one of the
} liver samples.
}
} The researcher asked "How do you know it's glycogen". I said
} "It just is".
} He is worried that his supervisor won't accept that it's
} glycogen just because we said so.
} He wants to prove it.
}
} So my question is...
}
} Is there a stain for epoxy resin sections that will stain
} specifically for glycogen?
} Will periodic acid Schiff (PAS) work on resin-embedded sections?
}
} Regards,
}
} John Brealey
}
} Supervisor - Electron Microscope Unit
}
}
} E john.brealey-at-imvs.sa.gov.au
}
} T 8222 6612
}
} F 8222 6425
}
} www.sapathology.sa.gov.au
}
}
}
} SA Pathology (Queen Elizabeth Hospital)
}
}
}
} Electron Microscope Unit, Surgical Pathology
}
} SA Pathology
}
} Queen Elizabeth Hospital
}
} Woodville, 5011
}
} AUSTRALIA
}
}
}
} Quality Pathology supporting Training and Research
}
}
}
} ==============================Original
} Headers==============================
} 25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009
} 25, 27 -- Received: from mailgate9.sa.gov.au
} (mailgate9.sa.gov.au [203.26.121.14])
} 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2O5qP3Q011161
} 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar
} 2009 00:52:26 -0500
} 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000";
} 25, 27 -- d="scan'208";a="16015375"
} 25, 27 -- Received: from unknown (HELO
} EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
} 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24
} Mar 2009 16:22:24 +1030
} 25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
} 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with
} Microsoft SMTP Server id
} 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030
} 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au
} [10.20.138.125]) by
} 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id
} E9FE02EC078 for
} 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009
} 16:22:23 +1030 (CST)
} 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
} 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
} 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue
} 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030
} 25, 27 -- Organization: IMVS
} 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i}
} 25, 27 -- MIME-Version: 1.0
} 25, 27 -- Content-Type: text/plain; charset="us-ascii"
} 25, 27 -- Content-Transfer-Encoding: 7bit
} 25, 27 -- X-Mailer: Microsoft Office Outlook 11
} 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ==
} 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
25, 36 -- From W.Muss-at-salk.at Tue Mar 24 04:38:39 2009
25, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
25, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O9ccGu009783
25, 36 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 04:38:38 -0500
25, 36 -- Received: from localhost (localhost [127.0.0.1])
25, 36 -- by hermes.salk.at (Postfix) with ESMTP id 87760C383D;
25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100 (CET)
25, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
25, 36 -- Received: from hermes.salk.at ([127.0.0.1])
25, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
25, 36 -- with ESMTP id bPNousV6-H1H; Tue, 24 Mar 2009 10:38:36 +0100 (CET)
25, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
25, 36 -- by hermes.salk.at (Postfix) with ESMTP id 1682FC382A;
25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100 (CET)
25, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100
25, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
25, 36 -- Content-class: urn:content-classes:message
25, 36 -- MIME-Version: 1.0
25, 36 -- Content-Type: text/plain;
25, 36 -- charset="iso-8859-1"
25, 36 -- Subject: [Microscopy] Re: Staining for glycogen in resin-embedded tissue
25, 36 -- Date: Tue, 24 Mar 2009 10:38:35 +0100
25, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D08F5-at-N1RZ116.lksdom21.lks.local}
25, 36 -- In-Reply-To: {200903240557.n2O5vGaM017473-at-ns.microscopy.com}
25, 36 -- X-MS-Has-Attach:
25, 36 -- X-MS-TNEF-Correlator:
25, 36 -- Thread-Topic: [Microscopy] Re: Staining for glycogen in resin-embedded tissue
25, 36 -- Thread-Index: AcmsRWZplxy2w6F9S2OGOqwAXifM9QAD2edg
25, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
25, 36 -- To: {john.brealey-at-imvs.sa.gov.au}
25, 36 -- Cc: {microscopy-at-microscopy.com}
25, 36 -- X-OriginalArrivalTime: 24 Mar 2009 09:38:36.0144 (UTC) FILETIME=[4DF5EF00:01C9AC64]
25, 36 -- X-Scanned-By: SALK-Content-Filter
25, 36 -- Content-Transfer-Encoding: 8bit
25, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2O9ccGu009783
==============================End of - Headers==============================




From: Diane.Ciaburri-at-gd-ais.com
Date: Tue, 24 Mar 2009 10:45:48 -0500
Subject: [Microscopy] SEM (Old) - Polaroid Type 53 Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was just going to replenish my stock of Polaroid Type 53 film (black & white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is not making it any more. Has anyone found a good substitute for this film? The ASA800 isn't important, a lower ASA is OK.

Thanks,

Diane Ciaburri
______________________________________
Diane Ciaburri
Principal Materials Engineer
General Dynamics
100 Plastics Ave., Rm 2517A
Pittsfield MA 01201
phone: (413) 494-3430
email: diane.ciaburri-at-gd-ais.com
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯


==============================Original Headers==============================
5, 26 -- From prvs=1328d24a38=diane.ciaburri-at-gd-ais.com Tue Mar 24 10:45:48 2009
5, 26 -- Received: from camv02-relay2.casc.gd-ais.com (CAMV02-RELAY2.CASC.GD-AIS.COM [192.5.164.99])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OFjkq7006338
5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 10:45:47 -0500
5, 26 -- Received: from ([10.73.100.22])
5, 26 -- by camv02-relay2.casc.gd-ais.com with ESMTP id 5202701.157164724;
5, 26 -- Tue, 24 Mar 2009 08:37:42 -0700
5, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by camv02-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.3959);
5, 26 -- Tue, 24 Mar 2009 08:45:17 -0700
5, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 26 -- Content-class: urn:content-classes:message
5, 26 -- MIME-Version: 1.0
5, 26 -- Content-Type: text/plain;
5, 26 -- charset="iso-8859-1"
5, 26 -- Subject: SEM (Old) - Polaroid Type 53 Film
5, 26 -- Date: Tue, 24 Mar 2009 11:45:14 -0400
5, 26 -- Message-ID: {1AFEAA87E04C0647A56AEA4BC5A73F3B03878B81-at-MAPF01-MAIL01.ad.gd-ais.com}
5, 26 -- X-MS-Has-Attach:
5, 26 -- X-MS-TNEF-Correlator:
5, 26 -- Thread-Topic: SEM (Old) - Polaroid Type 53 Film
5, 26 -- Thread-Index: Acmsl4YNYm/iHqxtSsaIeDSvZ6GQEA==
5, 26 -- From: "Ciaburri, Diane A." {Diane.Ciaburri-at-gd-ais.com}
5, 26 -- To: {Microscopy-at-microscopy.com}
5, 26 -- X-OriginalArrivalTime: 24 Mar 2009 15:45:17.0140 (UTC) FILETIME=[87938140:01C9AC97]
5, 26 -- Content-Transfer-Encoding: 8bit
5, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OFjkq7006338
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Tue, 24 Mar 2009 10:48:49 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tungsten gun ESEM
In a recent review someone said ESEM was like SEM but with extra difficulty. Not so much difficult as frustrating when viewing wet biological samples. Not being gold coated they give off fewer secondary electrons. At lower voltages the signal to noise ratio makes even modest magnifications hard to achieve. So, I confess, I have gone from being a 5kV high vacuum user to a 20kV wet ESEM user.

FEGESEM
Still problematic - Kirk et al. (2009)* in an excellent review advocate 5-7kV.

Dave

* Kirk SE, Skepper JN Donald AM. 2009 Application of environmental scanning electron microscopy to determine surface structure. J Microsc 233:205-244.


-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: 24 March 2009 08:39
To: David Patton

Hi all

In spite of beeing interesting by the concept of the ESEM since long
ago, I had until now nor much time neither the real need to work on one.
So I've a couple of questions about it.

1- First, most pictures done by ESEM in wet mode I've seen from
collegues, either biologists or from material science, are done at high
energy, 20-30 kV. It seems that the low energy "culture" is a bit
ignored. (On the other hand, I agree that I'm perheps a bit addict to
the low kV range !) But apart the subjecives aspects like "traditions",
lab "culture" or the need of EDS, is there a more objective and
technical reason for such choice ? Do the wet conditions limit so
drastically the performences of the ESEM at low energy (lower then 5 keV
f.ex.) ?

2- I a paper published in M&M 6-2000, Danilatos describes a very
interesting evolution of the PLA (in particular for people interested in
vaccum technology, like me...), called Reverse Flow Pressure Limiting
Apperture. An annular gas jet flows from an intermediate stage at higher
pressure into the specimen chamber, generating a pumping effect in the
central part of the PLA and allowding to work with a greater
differential pressure between the chamber and the column.
I asked me if such a device has been soon integrated in commercial
VP-SEMs, from FEI or other manufactuers. Danilatos mention too a
cooperation with Zeiss on his website, but I never heard about a
developpment like that by Zeiss.

Thanks for comments

Jacques

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 03:35:23 2009
9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.157])
9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O8ZMax011825
9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 03:35:23 -0500
9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 09:35:21 +0100 (CET)
9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 29 -- (No client certificate requested)
9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 5F52D3EC002
9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar 2009 09:34:14 +0100 (CET)
9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100
9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105)
9, 29 -- MIME-Version: 1.0
9, 29 -- To: microscopy-at-microscopy.com
9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 29 -- Content-Transfer-Encoding: 8bit
9, 29 -- X-IPCMS-MailScanner: Found to be clean
9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 29 -- Subject: Quest. about the ESEM
9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET)
9, 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 2009 on mr7.u-strasbg.fr
9, 29 -- X-Virus-Status: Clean
9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
9, 29 -- autolearn=disabled version=3.2.5
9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr7.u-strasbg.fr
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
24, 35 -- From David.Patton-at-uwe.ac.uk Tue Mar 24 10:48:49 2009
24, 35 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
24, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2OFmmSj008169
24, 35 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 10:48:49 -0500
24, 35 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
24, 35 -- id 0439_432848b4_188b_11de_8e25_00142223915c;
24, 35 -- Tue, 24 Mar 2009 15:48:47 +0000
24, 35 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
24, 35 -- (egen-uwe01.uwe.ac.uk [164.11.249.121])
24, 35 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
24, 35 -- 2005)) with SMTP id {0KH0009IAP99XX-at-mta01.uwe.ac.uk} for
24, 35 -- microscopy-at-microscopy.com; Tue, 24 Mar 2009 15:48:47 +0000 (GMT)
24, 35 -- Date: Tue, 24 Mar 2009 15:46:56 +0000
24, 35 -- From: David Patton {David.Patton-at-uwe.ac.uk}
24, 35 -- Subject: RE: [Microscopy] Quest. about the ESEM
24, 35 -- In-reply-to: {200903240839.n2O8dIrV017580-at-ns.microscopy.com}
24, 35 -- To: jacques.faerber-at-ipcms.u-strasbg.fr
24, 35 -- Message-id: {F247F674896BE243AD8263C5280E2BDB048C5343-at-egen-uwe01}
24, 35 -- MIME-version: 1.0
24, 35 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
24, 35 -- Content-type: text/plain;
24, 35 -- charset="utf-8"
24, 35 -- Content-class: urn:content-classes:message
24, 35 -- Thread-topic: [Microscopy] Quest. about the ESEM
24, 35 -- Thread-index: AcmsXAyOvW4jcJoZTHyOpXoHc1xlwAAOY6rw
24, 35 -- X-MS-Has-Attach:
24, 35 -- X-MS-TNEF-Correlator:
24, 35 -- References: {200903240839.n2O8dIrV017580-at-ns.microscopy.com}
24, 35 -- X-NAIMIME-Disclaimer: 1
24, 35 -- X-NAIMIME-Modified: 1
24, 35 -- X-NAI-Spam-Score: 0
24, 35 -- X-NAI-Spam-Rules: 1 Rules triggered
24, 35 -- RV3237=0
24, 35 -- Content-Transfer-Encoding: 8bit
24, 35 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2OFmmSj008169
==============================End of - Headers==============================




From: dhorne-at-interchange.ubc.ca
Date: Tue, 24 Mar 2009 12:08:47 -0500
Subject: [Microscopy] viaWWW: Fibrin clots- SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dhorne-at-interchange.ubc.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] Fibrin clots- SEM

Question: We have a student interested in looking at the morphologies
(fibre arrangement and spacing) of fibrin clots treated with various
stabilizing agents as a bulk sample, not as a thin layer. I have
tried a few different techniques- CPD vs HMDS, ROTO, holding the clot
between dialysis membranes in a cartridge arrangement (for the record
this was a miserable failure),and processing the clot formed in an
Eppendorf tube- but continue to have issues with collapse of the clot.

We've done a lit search but haven't been able to find a definitive
technique for a bulk clot. It is important to note this is an SEM
application, and that TEM micrographs are not what the student would
like to present in his paper.

I'm considering taking the clots to 100% EtOH and then freeze-drying,
but my gut tells me the clots will still collapse.

I am wondering if it is even possible to keep a bulk clot expanded?
Should fibrin be thought of as a cable, without strength in
compression? If so, how have people worked around this in the past?

Thanks,

Derrick Horne





Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Tue Mar 24 12:08:47 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OH8kPV005652
15, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 12:08:47 -0500
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240805c5eec3fcc4e0-at-[206.69.208.22]}
15, 11 -- Date: Tue, 24 Mar 2009 12:08:46 -0500
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Fibrin clots- SEM
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 24 Mar 2009 13:49:24 -0500
Subject: [Microscopy] Staining for glycogen in resin-embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes there is! Yes it will!
Actually I already answered the same question 3 weeks ago in this list.
I copy my answer here:




----- Original Message ----
X-from: "john.brealey-at-imvs.sa.gov.au" {john.brealey-at-imvs.sa.gov.au}
To: nizets2-at-yahoo.com
Sent: Tuesday, March 24, 2009 6:58:02 AM

Hi,

We have a researcher who is studying the effects of a particular drug on rat
liver, heart and spinal cord.
Tissue for EM was processed with osmium tetroxide, en-bloc stained with
uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy
resin. Thin sections were stained with lead citrate.
By EM we found increased amounts of glycogen in one of the liver samples.
The researcher asked "How do you know it's glycogen".
I said "It just is".
He is worried that his supervisor won't accept that it's glycogen just
because we said so. He wants to prove it.

So my question is...

Is there a stain for epoxy resin sections that will stain specifically for
glycogen?
Will periodic acid Schiff (PAS) work on resin-embedded sections?

Regards,

John Brealey


Supervisor - Electron Microscope Unit



E john.brealey-at-imvs.sa.gov.au

T 8222 6612

F 8222 6425

www.sapathology.sa.gov.au



SA Pathology (Queen Elizabeth Hospital)



Electron Microscope Unit, Surgical Pathology

SA Pathology

Queen Elizabeth Hospital

Woodville, 5011

AUSTRALIA



Quality Pathology supporting Training and Research



==============================Original Headers==============================
25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009
25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161
25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500
25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000";
25, 27 -- d="scan'208";a="16015375"
25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030
25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030
25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for
25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST)
25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
25, 27 -- Subject: Staining for glycogen in resin-embedded tissue
25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030
25, 27 -- Organization: IMVS
25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i}
25, 27 -- MIME-Version: 1.0
25, 27 -- Content-Type: text/plain; charset="us-ascii"
25, 27 -- Content-Transfer-Encoding: 7bit
25, 27 -- X-Mailer: Microsoft Office Outlook 11
25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ==
25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================





==============================Original Headers==============================
43, 23 -- From nizets2-at-yahoo.com Tue Mar 24 13:49:21 2009
43, 23 -- Received: from web110807.mail.gq1.yahoo.com (web110807.mail.gq1.yahoo.com [67.195.13.230])
43, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2OInJ0h024007
43, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 13:49:20 -0500
43, 23 -- Received: (qmail 90531 invoked by uid 60001); 24 Mar 2009 18:49:18 -0000
43, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237920558; bh=IQ4e4Pe+purZnbt8eOSy61OPZwfUSyyuUAa+7sFO8y0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type; b=M+5QbBWWPN3GpVXeIeWTRUfISLKh9yKZoS0pukgo/5p/trO3bJt3jqABZ0auzWRwKOnBX4wC++aAeQSgGcGVh9VsBiKbh+9R6KgOrMMqutwd0ePLJ4k3rVrpWTkj9gDd9lsV+pkmQxZW9b3CPNprD16F9IxAKsXMbH5RAAckUKE=
43, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
43, 23 -- s=s1024; d=yahoo.com;
43, 23 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type;
43, 23 -- b=YIomuWpn07SWizLL/F292HK6jAcX0FBhayFJ8gZzLkOWknL2NLYHGqNr/oC4QMoCjNOy3Ssoaq6/csKNcwdsjn3m2wyXcspX7lOKqdzTclpgF1vij65seiXjQUCp6O95G0ncf1ZZs8TjY53ccL5Kx2CFMrY069lns3LCWDVsLYg=;
43, 23 -- Message-ID: {152072.90140.qm-at-web110807.mail.gq1.yahoo.com}
43, 23 -- X-YMail-OSG: .V_2ey0VM1miD1JTsxxJvM_sTOcYBWrRjgL6rNp_00D_6HVNbVxva1ONO.Ygut.zsa5luEeh37sR3I5Lma.3IFGV2jweN9Dn7ZW1LHBgrRKkQXn2f_R9IkE9LTqfder3er_sZFgAcSjKEcJ2rW7UJUhNeRrFNatNevkAQ3RhUlLjOtREVIWcWhHQfJX9zqUEBU5qccP9wAAvlEmzdrpY8lbAModByseR
43, 23 -- Received: from [80.121.65.0] by web110807.mail.gq1.yahoo.com via HTTP; Tue, 24 Mar 2009 11:49:18 PDT
43, 23 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1
43, 23 -- References: {200903240558.n2O5w21r018563-at-ns.microscopy.com}
43, 23 -- Date: Tue, 24 Mar 2009 11:49:18 -0700 (PDT)
43, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com}
43, 23 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue
43, 23 -- To: john.brealey-at-imvs.sa.gov.au
43, 23 -- Cc: microscopy-at-microscopy.com
43, 23 -- In-Reply-To: {200903240558.n2O5w21r018563-at-ns.microscopy.com}
43, 23 -- MIME-Version: 1.0
43, 23 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 24 Mar 2009 13:58:02 -0500
Subject: [Microscopy] Staining for glycogen in resin-embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry I slipped.
Here is my answer:

I have asked the same question as you on the list some years ago and I got several answers.
Here
is the very straightforward protocol I finally used on my EPON sections
of intestine and it worked, although the staining was not very intense.
I didnt really spend much time to improve the method though.

- Periodic acid 5%: 30 min at 50°C
- Schiff Reagent: 30 min at 50°C
- Post-staining: Azur II mix (it is a 1:1 mix of methylene blue and Azur II, more stable than methylene alone): 20 min RT
(section thickness: 300 nm)

Additionally, one person told me he stained the glycogen using reduced osmium.
Another one gave me this reference:
Shroeder et al. (1980) "An established routine method for differential staining of epoxy-embedded tissue sections"
Microscopia Acta 83,111-116

Best of luck,

Stephane



----- Original Message ----
X-from: "john.brealey-at-imvs.sa.gov.au" {john.brealey-at-imvs.sa.gov.au}
To: nizets2-at-yahoo.com
Sent: Tuesday, March 24, 2009 6:58:02 AM

Hi,

We have a researcher who is studying the effects of a particular drug on rat
liver, heart and spinal cord.
Tissue for EM was processed with osmium tetroxide, en-bloc stained with
uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy
resin. Thin sections were stained with lead citrate.
By EM we found increased amounts of glycogen in one of the liver samples.
The researcher asked "How do you know it's glycogen".
I said "It just is".
He is worried that his supervisor won't accept that it's glycogen just
because we said so. He wants to prove it.

So my question is...

Is there a stain for epoxy resin sections that will stain specifically for
glycogen?
Will periodic acid Schiff (PAS) work on resin-embedded sections?

Regards,

John Brealey


Supervisor - Electron Microscope Unit



E john.brealey-at-imvs.sa.gov.au

T 8222 6612

F 8222 6425

www.sapathology.sa.gov.au



SA Pathology (Queen Elizabeth Hospital)



Electron Microscope Unit, Surgical Pathology

SA Pathology

Queen Elizabeth Hospital

Woodville, 5011

AUSTRALIA



Quality Pathology supporting Training and Research



==============================Original Headers==============================
25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009
25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161
25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500
25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000";
25, 27 -- d="scan'208";a="16015375"
25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030
25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030
25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for
25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST)
25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
25, 27 -- Subject: Staining for glycogen in resin-embedded tissue
25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030
25, 27 -- Organization: IMVS
25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i}
25, 27 -- MIME-Version: 1.0
25, 27 -- Content-Type: text/plain; charset="us-ascii"
25, 27 -- Content-Transfer-Encoding: 7bit
25, 27 -- X-Mailer: Microsoft Office Outlook 11
25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ==
25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================






==============================Original Headers==============================
48, 25 -- From nizets2-at-yahoo.com Tue Mar 24 13:58:02 2009
48, 25 -- Received: from web110815.mail.gq1.yahoo.com (web110815.mail.gq1.yahoo.com [67.195.13.238])
48, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2OIw0i4029486
48, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 13:58:01 -0500
48, 25 -- Received: (qmail 40779 invoked by uid 60001); 24 Mar 2009 18:57:59 -0000
48, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237921078; bh=v22cQOqsnLT0xnbHVtJl5ZoNU8z6w4f6SyoXMD0AR1o=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=nNKR2ycOY8LhCon+Xc25bjQciGhNNgNyMj1Vr5cb54W3mlJQlRfcBkMBVzr1Z+/clzH9M3JHZOa7BoAbNDGIYFsO/uB3WhJNKn2Vs0vcaYOrL1ctEjFpIonUhpGWjC2ieFeZvfSGNMV/XZiPUGf7iWLETGCnrAB1zujoMI+AbWI=
48, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
48, 25 -- s=s1024; d=yahoo.com;
48, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
48, 25 -- b=14YObwpu+I9PJe1nb/z2SQfdqb3x24t4VsxBCLGRzzlEzqeFfB7b1kAjfV049xJpNvCTS6SIK0QsxKcKOMqUAIZPLXftkjOKsN8fBltwMpAc0sLUP5cgk/2uSXAok6sphZKnJMOX67IIx58kFLAg14YnJmjZU/eRhRWvoAsGc8s=;
48, 25 -- Message-ID: {983013.37372.qm-at-web110815.mail.gq1.yahoo.com}
48, 25 -- X-YMail-OSG: 6UTxgt8VM1kLAAOA9PsuYwPOqCaE.ygC7Sh1LGAaxDHAp7w3q7vOYxwAYcZEDkrVconjWnFVX0lq5Fa9GLdDLc5xsrGIp8OfW5aWBVvilNGAG77s_kFgxZ1siLn714FT840h.oDmadZC772k1jwM4PTAbbypzYcEbrvmdo0.3cbfVxWtJ8nzfSKeu.32Q6QEyya0UfjyqTlSs45ocNhc_L4MIPT8AC.EMZ6EzWk6Aa4lrRbI5upX1ac.E99eYylULlsuwoM5b5nXumif6x7aA_8-
48, 25 -- Received: from [80.121.65.0] by web110815.mail.gq1.yahoo.com via HTTP; Tue, 24 Mar 2009 11:57:58 PDT
48, 25 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1
48, 25 -- References: {200903240558.n2O5w21r018563-at-ns.microscopy.com}
48, 25 -- Date: Tue, 24 Mar 2009 11:57:58 -0700 (PDT)
48, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
48, 25 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue
48, 25 -- To: john.brealey-at-imvs.sa.gov.au
48, 25 -- Cc: microscopy-at-microscopy.com
48, 25 -- In-Reply-To: {200903240558.n2O5w21r018563-at-ns.microscopy.com}
48, 25 -- MIME-Version: 1.0
48, 25 -- Content-Type: text/plain; charset=iso-8859-1
48, 25 -- Content-Transfer-Encoding: 8bit
48, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OIw0i4029486
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 24 Mar 2009 14:26:22 -0500
Subject: [Microscopy] SEM (Old) - Polaroid Type 53 Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diane,
Your choice is digital capture or ... digital capture. If you have an EDS
system, you may already have digital capture capability. There are some
digital camera set-ups that keep using your record CRT or you can go to
passive or active digital capture.

If you are interested in more info, please contact me off list.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: Diane.Ciaburri-at-gd-ais.com [mailto:Diane.Ciaburri-at-gd-ais.com]
Sent: Tuesday, March 24, 2009 11:53 AM
To: kenconverse-at-qualityimages.biz


I was just going to replenish my stock of Polaroid Type 53 film (black &
white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is
not making it any more. Has anyone found a good substitute for this film?
The ASA800 isn't important, a lower ASA is OK.

Thanks,

Diane Ciaburri
______________________________________
Diane Ciaburri
Principal Materials Engineer
General Dynamics
100 Plastics Ave., Rm 2517A
Pittsfield MA 01201
phone: (413) 494-3430
email: diane.ciaburri-at-gd-ais.com
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯


==============================Original Headers==============================
5, 26 -- From prvs=1328d24a38=diane.ciaburri-at-gd-ais.com Tue Mar 24 10:45:48
2009
5, 26 -- Received: from camv02-relay2.casc.gd-ais.com
(CAMV02-RELAY2.CASC.GD-AIS.COM [192.5.164.99])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2OFjkq7006338
5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 10:45:47
-0500
5, 26 -- Received: from ([10.73.100.22])
5, 26 -- by camv02-relay2.casc.gd-ais.com with ESMTP id
5202701.157164724;
5, 26 -- Tue, 24 Mar 2009 08:37:42 -0700
5, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by
camv02-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.3959);
5, 26 -- Tue, 24 Mar 2009 08:45:17 -0700
5, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 26 -- Content-class: urn:content-classes:message
5, 26 -- MIME-Version: 1.0
5, 26 -- Content-Type: text/plain;
5, 26 -- charset="iso-8859-1"
5, 26 -- Subject: SEM (Old) - Polaroid Type 53 Film
5, 26 -- Date: Tue, 24 Mar 2009 11:45:14 -0400
5, 26 -- Message-ID:
{1AFEAA87E04C0647A56AEA4BC5A73F3B03878B81-at-MAPF01-MAIL01.ad.gd-ais.com}
5, 26 -- X-MS-Has-Attach:
5, 26 -- X-MS-TNEF-Correlator:
5, 26 -- Thread-Topic: SEM (Old) - Polaroid Type 53 Film
5, 26 -- Thread-Index: Acmsl4YNYm/iHqxtSsaIeDSvZ6GQEA==
5, 26 -- From: "Ciaburri, Diane A." {Diane.Ciaburri-at-gd-ais.com}
5, 26 -- To: {Microscopy-at-microscopy.com}
5, 26 -- X-OriginalArrivalTime: 24 Mar 2009 15:45:17.0140 (UTC)
FILETIME=[87938140:01C9AC97]
5, 26 -- Content-Transfer-Encoding: 8bit
5, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2OFjkq7006338
==============================End of - Headers==============================




==============================Original Headers==============================
18, 25 -- From kenconverse-at-qualityimages.biz Tue Mar 24 14:26:21 2009
18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OJQKRu020438
18, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 14:26:21 -0500
18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
18, 25 -- with ESMTP
18, 25 -- id {20090324192618.GWWR22116.cdptpa-omta02.mail.rr.com-at-Ken} ;
18, 25 -- Tue, 24 Mar 2009 19:26:18 +0000
18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
18, 25 -- To: {Diane.Ciaburri-at-gd-ais.com} , "MSA Listserver" {microscopy-at-microscopy.com}
18, 25 -- Subject: RE: [Microscopy] SEM (Old) - Polaroid Type 53 Film
18, 25 -- Date: Tue, 24 Mar 2009 15:26:13 -0400
18, 25 -- Message-ID: {FF845D85C5EE430FBA5FC4F7A87F71BE-at-Ken}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain;
18, 25 -- charset="iso-8859-1"
18, 25 -- X-Priority: 3 (Normal)
18, 25 -- X-MSMail-Priority: Normal
18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
18, 25 -- Importance: Normal
18, 25 -- Thread-Index: AcmsmJO2nm7YAX6eS5ucdI7JNmeEywAHR1Mg
18, 25 -- In-Reply-To: {200903241552.n2OFqjwg014445-at-ns.microscopy.com}
18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
18, 25 -- Content-Transfer-Encoding: 8bit
18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OJQKRu020438
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 24 Mar 2009 14:38:11 -0500
Subject: [Microscopy] Benchtop CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ritchie,
Meant to get back to you sooner on this. The cheapest way to get to CL is
to simply remove the Faraday Cage and scintillator from the secondary
detector. That is your basic CL detector (monochrome). A dedicated CL
detector generally has a larger diameter light pipe, but is still basically
the back end of an E-T detector.

A color CL detector is a whole other can of worms and I don't know of a
"cheap" way there.

I don't see why a CL detector couldn't be put on a desktop SEM, if there is
a suitable port available and it can handle additional video inputs.

Have fun!

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz]
Sent: Wednesday, March 18, 2009 9:30 PM
To: kenconverse-at-qualityimages.biz

Hi again

What's the cheapest way of getting into CL of quartz? Can CL be put onto a
benchtop SEM?

cheers

Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
6, 28 -- From r.sims-at-auckland.ac.nz Wed Mar 18 20:28:01 2009
6, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz
[130.216.12.33])
6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2J1RvM5030297
6, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 20:28:01
-0500
6, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
1BE859DFD1
6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:56
+1300 (NZDT)
6, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
6, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
6, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1])
(amavisd-new, port 10024)
6, 28 -- with ESMTP id IwqwR4nhAL-X for {microscopy-at-Microscopy.Com} ;
6, 28 -- Thu, 19 Mar 2009 14:27:56 +1300 (NZDT)
6, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz
[130.216.59.18])
6, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
6, 28 -- (No client certificate requested)
6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
EC4C59DFCB
6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:55
+1300 (NZDT)
6, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
6, 28 -- To: microscopy-at-Microscopy.Com
6, 28 -- Date: Thu, 19 Mar 2009 14:30:10 +1300
6, 28 -- MIME-Version: 1.0
6, 28 -- Subject: Benchtop CL
6, 28 -- Message-ID: {49C256F2.21397.17DDAB3-at-r.sims.auckland.ac.nz}
6, 28 -- Priority: normal
6, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
6, 28 -- Content-type: text/plain; charset=US-ASCII
6, 28 -- Content-transfer-encoding: 7BIT
6, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




==============================Original Headers==============================
21, 25 -- From kenconverse-at-qualityimages.biz Tue Mar 24 14:38:11 2009
21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OJcB0i002307
21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 14:38:11 -0500
21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
21, 25 -- with ESMTP
21, 25 -- id {20090324193810.ECBC6142.cdptpa-omta03.mail.rr.com-at-Ken} ;
21, 25 -- Tue, 24 Mar 2009 19:38:10 +0000
21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 25 -- To: {r.sims-at-auckland.ac.nz} , "MSA Listserver" {microscopy-at-microscopy.com}
21, 25 -- Subject: RE: [Microscopy] Benchtop CL
21, 25 -- Date: Tue, 24 Mar 2009 15:38:06 -0400
21, 25 -- Message-ID: {C6DC472AAED54DE9B8A9D1F9C0A08D1D-at-Ken}
21, 25 -- MIME-Version: 1.0
21, 25 -- Content-Type: text/plain;
21, 25 -- charset="us-ascii"
21, 25 -- X-Priority: 3 (Normal)
21, 25 -- X-MSMail-Priority: Normal
21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 25 -- Importance: Normal
21, 25 -- Thread-Index: AcmoMkI+rHxXD/2dRDCCNeikjP4PbAEhGviw
21, 25 -- In-Reply-To: {200903190130.n2J1UF6K001811-at-ns.microscopy.com}
21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 25 -- Content-Transfer-Encoding: 8bit
21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OJcB0i002307
==============================End of - Headers==============================




From: abrun-at-hsc.unt.edu
Date: Tue, 24 Mar 2009 15:38:33 -0500
Subject: [Microscopy] viaWWW: TEM digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abrun-at-hsc.unt.edu
Name: Anne-Marie Brun

Organization: UNT HSC at Fort Worth Texas 76107, USA

Title-Subject: [Filtered] digital cameras

Question: Hello everyone,
We would like to switch from negative plates to digital capture. Our
TEM is a Zeiss EM 910 instrument. We would like to have a digital
camera with at least the same resolution as the film has.
I'm sure there are several people out there who had to switch over to
digital as we plan to do. What is the best way and what is the best
camera and best price?

Thanks for your help. It is always good to listen to experts.
Anne-Marie

Login Host: 129.120.99.57
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Mar 24 15:38:33 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OKcWc5017885
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 15:38:32 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c5eef5294bd7-at-[206.69.208.22]}
7, 11 -- Date: Tue, 24 Mar 2009 15:38:32 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: TEM digital cameras
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jerzy.gazda-at-ceriumlabs.com
Date: Tue, 24 Mar 2009 16:25:36 -0500
Subject: [Microscopy] Hitachi S4800 SEM external control to stage and image capture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

I am interested in opinions on any available external systems (scan generators and stage controllers) that can be attached to Hitachi S4800 SEM.

We are trying to implement a system with external control of a piezo stage that would piggy-back on the main stage of the microscope. I know IXRF and Thermo offers this as a part of their EDS package and other EDS vendors might have similar systems for their mapping acquisitions. This is one route, what are the others? PI and attoCube have piezo-stages with controllers but no interface to a SEM software, are there others on the market with similar products?

Thanks in advance for you suggestions,


Jerzy
***************************************************
Jerzy Gazda Ph.D.
Section Manager - TEM, FIB, SEM
Cerium Laboratories LLC
5204 E. Ben White Blvd. MS-512
Austin, TX 78741
 
(512) 934-5185 vm
(512) 622-6600 pgr
 
www.ceriumlabs.com



==============================Original Headers==============================
8, 26 -- From Jerzy.Gazda-at-spansion.com Tue Mar 24 16:25:36 2009
8, 26 -- Received: from usausmgw01.spansion.com (usausmgw01.spansion.com [12.110.209.161])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OLPaCt000501
8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:25:36 -0500
8, 26 -- X-IronPort-AV: E=McAfee;i="5300,2777,5563"; a="3788966"
8, 26 -- Received: from usausexbh1.spansion.com ([10.248.26.58])
8, 26 -- by usausmgw01.spansion.com with ESMTP; 24 Mar 2009 14:25:35 -0700
8, 26 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959);
8, 26 -- Tue, 24 Mar 2009 16:25:35 -0500
8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 26 -- Content-class: urn:content-classes:message
8, 26 -- MIME-Version: 1.0
8, 26 -- Content-Type: text/plain;
8, 26 -- charset="iso-8859-1"
8, 26 -- Subject: Hitachi S4800 SEM external control to stage and image capture
8, 26 -- Date: Tue, 24 Mar 2009 16:25:35 -0500
8, 26 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158738596-at-USAUSEXMBPF1.spansion.com}
8, 26 -- X-MS-Has-Attach:
8, 26 -- X-MS-TNEF-Correlator:
8, 26 -- Thread-Topic: Hitachi S4800 SEM external control to stage and image capture
8, 26 -- Thread-Index: AcmsxxH61jPUnNX6RNC0fjsi4LpLMg==
8, 26 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com}
8, 26 -- To: {microscopy-at-microscopy.com}
8, 26 -- X-OriginalArrivalTime: 24 Mar 2009 21:25:35.0948 (UTC) FILETIME=[122258C0:01C9ACC7]
8, 26 -- Content-Transfer-Encoding: 8bit
8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OLPaCt000501
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Tue, 24 Mar 2009 16:46:07 -0500
Subject: [Microscopy] RE: SEM (Old) - Polaroid Type 53 Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diane,

Fuji makes such a product. More expensive and it may require that you
purchase a different holder. Check this out:

fujifilmusa.com/products/professional_photography/film/fujifilm_instant_films/fp_100b/index.html

John Bozzola



} Diane,
} Your choice is digital capture or ... digital capture. If you have an EDS
} system, you may already have digital capture capability. There are some
} digital camera set-ups that keep using your record CRT or you can go to
} passive or active digital capture.
}
} If you are interested in more info, please contact me off list.
}
} Ken Converse
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: Diane.Ciaburri-at-gd-ais.com [mailto:Diane.Ciaburri-at-gd-ais.com]
} Sent: Tuesday, March 24, 2009 11:53 AM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] SEM (Old) - Polaroid Type 53 Film
}
}
}
} I was just going to replenish my stock of Polaroid Type 53 film (black &
} white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is
} not making it any more. Has anyone found a good substitute for this film?
} The ASA800 isn't important, a lower ASA is OK.
}
} Thanks,
}
} Diane Ciaburri
} ______________________________________
} Diane Ciaburri
} Principal Materials Engineer
} General Dynamics
} 100 Plastics Ave., Rm 2517A
} Pittsfield MA 01201
} phone: (413) 494-3430
} email: diane.ciaburri-at-gd-ais.com


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
11, 17 -- From bozzola-at-siu.edu Tue Mar 24 16:46:07 2009
11, 17 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OLk73S014823
11, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:46:07 -0500
11, 17 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
11, 17 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n2OLk50R012948
11, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:46:06 -0500 (CDT)
11, 17 -- Mime-Version: 1.0
11, 17 -- Message-Id: {a0624080ac5ef04f0403d-at-[131.230.177.136]}
11, 17 -- Date: Tue, 24 Mar 2009 16:46:05 -0500
11, 17 -- To: Microscopy-at-microscopy.com
11, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
11, 17 -- Subject: [Microscopy] RE: SEM (Old) - Polaroid Type 53 Film
11, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
11, 17 -- X-Spam-Score: 0.00%
11, 17 -- X-MASF: 0.00%
11, 17 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Wed, 25 Mar 2009 04:30:32 -0500
Subject: [Microscopy] viaWWW: TEM digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Derrick
During dehydration and drying the clot will collapse anyway. That is
inevitable, unless you examine the specimen in wet conditions with ESEM, as
people currently discuss in our listserver. In SEM, if the student wants to
take measurements on the fibrin mesh he has to consider a ca 40% shrinkage
of the specimen. To keep the mesh close to the original shape I think you
have to attach the clot to a substrate that will also shrink -ideally some
soft animal tissue- so it will not distort the mesh to any direction. Or,
what about if you take out some red blood cells and leave more plasma to
coagulate, dehydrate and CPD the bulk clot and then you look in SEM only at
the the periphery of the clot -maybe that will be fine.
Good luck!
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477




----- Original Message -----
X-from: {dhorne-at-interchange.ubc.ca}
To: {eikonika-at-otenet.gr}
Sent: Tuesday, March 24, 2009 7:19 PM

Hi

Just a few notes on digital systems as related to real life!

We have a "do it yourself" course that requires the client to take images of
specific specimens and return the micrographs to us. Whilst most are able
to have a pretty good shot at reaching the standard we require, I had a
client who always turned out pictures which were identical, pictures that
did not show the subtle changes that the practical was designed to produce.

Eventually on visiting the customer we found the images on screen to be
superb and had they used sheet film the images would have been re produced
on the film. Unfortunately they used a digital camera mounted above the
viewing chamber which with its resolution and reduction in magnification was
unable to define the differences that we required between the micrographs.

The above was very worrying! The aim of the practicals was to teach
operators how the adjustment of the second condenser lens and determining
the correct spot size has a considerable effect on the quality of the image.
In addition basic Fresnel fringe images were not possible so poor was the
resolution.

A warning, to ensure any level of image quality go for the maximum pixels in
your camera if you intend to purchase an above screen side entry version.

Steve

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com
-----Original Message-----
X-from: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu]
Sent: 24 March 2009 20:40
To: protrain-at-emcourses.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: abrun-at-hsc.unt.edu
Name: Anne-Marie Brun

Organization: UNT HSC at Fort Worth Texas 76107, USA

Title-Subject: [Filtered] digital cameras

Question: Hello everyone,
We would like to switch from negative plates to digital capture. Our
TEM is a Zeiss EM 910 instrument. We would like to have a digital
camera with at least the same resolution as the film has.
I'm sure there are several people out there who had to switch over to
digital as we plan to do. What is the best way and what is the best
camera and best price?

Thanks for your help. It is always good to listen to experts.
Anne-Marie

Login Host: 129.120.99.57
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Mar 24 15:38:33 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2OKcWc5017885
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 15:38:32
-0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c5eef5294bd7-at-[206.69.208.22]}
7, 11 -- Date: Tue, 24 Mar 2009 15:38:32 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: TEM digital cameras
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 27 -- From protrain-at-emcourses.com Wed Mar 25 04:30:31 2009
20, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2P9UUaY030132
20, 27 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 04:30:31 -0500
20, 27 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:1524 helo=HP6220)
20, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
20, 27 -- (envelope-from {protrain-at-emcourses.com} )
20, 27 -- id 1LmPRK-0008zC-PM by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 11:30:27 +0200
20, 27 -- Reply-To: {protrain-at-emcourses.com}
20, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com}
20, 27 -- To: {abrun-at-hsc.unt.edu}
20, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
20, 27 -- References: {200903242039.n2OKdb85019286-at-ns.microscopy.com}
20, 27 -- Subject: RE: [Microscopy] viaWWW: TEM digital cameras
20, 27 -- Date: Wed, 25 Mar 2009 09:30:24 -0000
20, 27 -- Organization: Protrain
20, 27 -- Message-ID: {015201c9ad2c$55aff150$0200a8c0-at-HP6220}
20, 27 -- MIME-Version: 1.0
20, 27 -- Content-Type: text/plain;
20, 27 -- charset="us-ascii"
20, 27 -- Content-Transfer-Encoding: 7bit
20, 27 -- X-Mailer: Microsoft Office Outlook 11
20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
20, 27 -- Thread-Index: AcmswLSSN55/hVr2R3KIuEYsbsyUdAAaRGkg
20, 27 -- In-Reply-To: {200903242039.n2OKdb85019286-at-ns.microscopy.com}
20, 27 -- X-Scan-Signature: 45388ccb2d7502dca2b2dfcc51200f4d{92}}
20, 27 -- X-Trace: smtp01.dial-up.net 1LmPRK-0008zC-PM b53a054cb88295b81a0d93f861b3fcea
==============================End of - Headers==============================




From: ahmad_ds-at-yahoo.com
Date: Wed, 25 Mar 2009 07:16:51 -0500
Subject: [Microscopy] viaWWW: EDS window

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkhaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] EDS window

Question: I work on and EDS manufactured by EDAX of the model XL-30
SUTW. I want to know what is the material of fabrication of the super
ultra-thin window of the EDS I have.

Thank you.

Login Host: 87.236.233.99
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 07:16:50 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PCGmWA019489
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 07:16:49 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p0624080bc5efd11fda7e-at-[206.69.208.22]}
7, 11 -- Date: Wed, 25 Mar 2009 07:16:47 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: EDS window
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Wed, 25 Mar 2009 08:19:37 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi
I have frequently imaged wet samples at 5kV and image quality can be variable depending on the sample. Very flat well attached cells on glass are some of the more problematic. But using higher kV means you hardly see the cells.
However just yesterday I looked at some confluent layers of mineralising cells on thermanox at 5kV and 4-5 torr with little difficulty (but it is a FEG)
Always ask researchers for extra specimens if possible to play with and optimise conditions. I routinely start low and work up to higher kV if necessary.

------------

Tungsten gun ESEM
In a recent review someone said ESEM was like SEM but with extra difficulty. Not so much difficult as frustrating when viewing wet biological samples. Not being gold coated they give off fewer secondary electrons. At lower voltages the signal to noise ratio makes even modest magnifications hard to achieve. So, I confess, I have gone from being a 5kV high vacuum user to a 20kV wet ESEM user.

FEGESEM
Still problematic - Kirk et al. (2009)* in an excellent review advocate 5-7kV.

Dave

* Kirk SE, Skepper JN Donald AM. 2009 Application of environmental scanning electron microscopy to determine surface structure. J Microsc 233:205-244.


-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: 24 March 2009 08:39
To: David Patton

Hi all

In spite of beeing interesting by the concept of the ESEM since long
ago, I had until now nor much time neither the real need to work on one.
So I've a couple of questions about it.

1- First, most pictures done by ESEM in wet mode I've seen from
collegues, either biologists or from material science, are done at high
energy, 20-30 kV. It seems that the low energy "culture" is a bit
ignored. (On the other hand, I agree that I'm perheps a bit addict to
the low kV range !) But apart the subjecives aspects like "traditions",
lab "culture" or the need of EDS, is there a more objective and
technical reason for such choice ? Do the wet conditions limit so
drastically the performences of the ESEM at low energy (lower then 5 keV
f.ex.) ?

2- I a paper published in M&M 6-2000, Danilatos describes a very
interesting evolution of the PLA (in particular for people interested in
vaccum technology, like me...), called Reverse Flow Pressure Limiting
Apperture. An annular gas jet flows from an intermediate stage at higher
pressure into the specimen chamber, generating a pumping effect in the
central part of the PLA and allowding to work with a greater
differential pressure between the chamber and the column.
I asked me if such a device has been soon integrated in commercial
VP-SEMs, from FEI or other manufactuers. Danilatos mention too a
cooperation with Zeiss on his website, but I never heard about a
developpment like that by Zeiss.

Thanks for comments

Jacques

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
23, 32 -- From Nicola.Weston-at-nottingham.ac.uk Wed Mar 25 08:19:36 2009
23, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5])
23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PDJY0l003066
23, 32 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 08:19:35 -0500
23, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11])
23, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
23, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
23, 32 -- id 1LmT0E-0003PW-4O
23, 32 -- for microscopy-at-microscopy.com; Wed, 25 Mar 2009 13:18:42 +0000
23, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
23, 32 -- Wed, 25 Mar 2009 13:18:40 +0000
23, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5
23, 32 -- Content-class: urn:content-classes:message
23, 32 -- MIME-Version: 1.0
23, 32 -- Content-Type: text/plain;
23, 32 -- charset="iso-8859-1"
23, 32 -- Subject: Quest. about the ESEM
23, 32 -- Date: Wed, 25 Mar 2009 13:18:40 -0000
23, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D940120F1E2-at-VUIEXCHA.ad.nottingham.ac.uk}
23, 32 -- X-MS-Has-Attach:
23, 32 -- X-MS-TNEF-Correlator:
23, 32 -- Thread-Topic: Quest. about the ESEM
23, 32 -- Thread-Index: AcmtTDaSAkqv7pZtTqqlHCQUXxTF+w==
23, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
23, 32 -- To: {microscopy-at-microscopy.com}
23, 32 -- X-OriginalArrivalTime: 25 Mar 2009 13:18:40.0992 (UTC) FILETIME=[37139600:01C9AD4C]
23, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
23, 32 -- X-UoN-MailScanner: Found to be clean
23, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
23, 32 -- X-Spam-Status: No
23, 32 -- Content-Transfer-Encoding: 8bit
23, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PDJY0l003066
==============================End of - Headers==============================




From: wendy.cheng-at-ipaper.com
Date: Wed, 25 Mar 2009 08:45:41 -0500
Subject: [Microscopy] viaWWW: Technique for Preparation of Polymer Film Cross Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wendy.cheng-at-ipaper.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: wendy.cheng-at-ipaper.com
Name: Wendy Cheng

Organization: International Paper

Title-Subject: [Filtered] Technique for Preparation of Polymer Film
Cross Sections

Question: Does anyone have a good technique for preparation of (cryo)
cross sections of polymer films for SEM examination? I am interested
in viewing the distribution of two polymers in a polymer blend.

Wendy Cheng, Ph.D.
International Paper
6283 Tri-Ridge Blvd.
Loveland, OH 45140

Phone: 513-248-6698
email: wendy.cheng-at-ipaper.com

Login Host: 141.129.1.98
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 08:45:39 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PDjbTk017677
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 08:45:38 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p0624080dc5efe5f4bc62-at-[206.69.208.22]}
8, 11 -- Date: Wed, 25 Mar 2009 08:45:36 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: wendy.cheng-at-ipaper.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Technique for Preparation of Polymer Film Cross Sections
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 25 Mar 2009 09:12:50 -0500
Subject: [Microscopy] Re: viaWWW: Technique for Preparation of Polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wendy,

I've always just dropped the polymer samples in liquid nitrogen,
grabbed and snapped.
I did have one nylon sample that required repeated flexing before it
would break, even at LN2 temperatures, but I still got good fractures
for examing the cross-section.
For thin films ... on paper? Snapping in LN2 worked there as well. If
the films are separate, then you may need to clamp them between two
thicker (but not thick) supporting pieces and snap the sandwich. This
also works for thin films laminated (with bonding) between two
thicker polymer pieces, although they will often delaminate (which
can be useful).
For films on substrates, it can make a difference whether the samples
are snapped "toward" or "away" from the film, that is, whether the
film is broken in compression ("toward") or tension ("away"). I
can't give a generic one is better than the other, but I try breaking
the film in tension first.
Thick(ish) substrates may require cutting/scoring/sawing 1/2 way (or
more) through first, from the side opposite the film.

Phil

} Email: wendy.cheng-at-ipaper.com
} Name: Wendy Cheng
}
} Organization: International Paper
}
} Title-Subject: [Filtered] Technique for Preparation of Polymer Film
} Cross Sections
}
} Question: Does anyone have a good technique for preparation of (cryo)
} cross sections of polymer films for SEM examination? I am interested
} in viewing the distribution of two polymers in a polymer blend.
}
} Wendy Cheng, Ph.D.
} International Paper
} 6283 Tri-Ridge Blvd.
} Loveland, OH 45140
}
} Phone: 513-248-6698
} email: wendy.cheng-at-ipaper.com
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 27 -- From oshel1pe-at-cmich.edu Wed Mar 25 09:12:49 2009
4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PECm6b032063
4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 09:12:49 -0500
4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n2PECd56005028;
4, 27 -- Wed, 25 Mar 2009 10:12:40 -0400
4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 27 -- Wed, 25 Mar 2009 10:12:33 -0400
4, 27 -- Mime-Version: 1.0
4, 27 -- Message-Id: {f06240800c5efe99479c2-at-[141.209.160.249]}
4, 27 -- In-Reply-To: {200903251350.n2PDoDqi023551-at-ns.microscopy.com}
4, 27 -- References: {200903251350.n2PDoDqi023551-at-ns.microscopy.com}
4, 27 -- Date: Wed, 25 Mar 2009 10:12:31 -0400
4, 27 -- To: wendy.cheng-at-ipaper.com
4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 27 -- Subject: Re: [Microscopy] viaWWW: Technique for Preparation of Polymer
4, 27 -- Film Cross Sections
4, 27 -- Cc: Microscopy-at-microscopy.com
4, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 27 -- X-OriginalArrivalTime: 25 Mar 2009 14:12:33.0204 (UTC) FILETIME=[BD9FFF40:01C9AD53]
4, 27 -- X-Canit-CHI2: 0.00
4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
4, 27 -- X-CanItPRO-Stream: default
4, 27 -- X-Canit-Stats-ID: 10755722 - 7e12acab6491
4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Wed, 25 Mar 2009 09:19:06 -0500
Subject: [Microscopy] Re: viaWWW: Technique for Preparation of Polymer Film Cross

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Wendy,
At the tire company I worked at we used to mount samples in a puddle of
mucilage gun and water (50/50) and freeze with LN2. The samples were
cut with glass knives for thin sections, but we also used a glass knife
to level the surface for SEM. You have to work quickly as the
brass/Teflon mounting block was the only source of cold, if one can
speak of a source of cold.

We also used a cryo-microtome in the same manner. Mount sample in the
water mucilage media, freeze and face off the block with a diamond
knife.

I believed we used a 50/50 water solution of DMSO to lubricate the
diamond edge.

Most things should cut well if you’re below the Tg point. Most plastics
have a Tg above room temp. Have fun…

I would suspect that if you are looking for phase morphology, you might
want to try etching one phase over the other, see Sawyer’s book on
polymer microscopy. I’ve had some success with vapor phase staining
with OsO4, assuming some unsaturation in one of the polymer phases.

stay safe........
Frank





wendy.cheng-at-ipape
r.com
To
03/25/2009 09:54 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] viaWWW: Technique for
wendy.cheng-at-ipape Preparation of Polymer Film Cross
r.com Sections













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both wendy.cheng-at-ipaper.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: wendy.cheng-at-ipaper.com
Name: Wendy Cheng

Organization: International Paper

Title-Subject: [Filtered] Technique for Preparation of Polymer Film
Cross Sections

Question: Does anyone have a good technique for preparation of (cryo)
cross sections of polymer films for SEM examination? I am interested
in viewing the distribution of two polymers in a polymer blend.

Wendy Cheng, Ph.D.
International Paper
6283 Tri-Ridge Blvd.
Loveland, OH 45140

Phone: 513-248-6698
email: wendy.cheng-at-ipaper.com

Login Host: 141.129.1.98
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 08:45:39 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2PDjbTk017677
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009
08:45:38 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p0624080dc5efe5f4bc62-at-[206.69.208.22]}
8, 11 -- Date: Wed, 25 Mar 2009 08:45:36 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: wendy.cheng-at-ipaper.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Technique for Preparation of Polymer Film Cross
Sections
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
30, 25 -- From frank_karl-at-lincolnelectric.com Wed Mar 25 09:19:06 2009
30, 25 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PEJ55k008985
30, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 09:19:05 -0500
30, 25 -- In-Reply-To: {200903251354.n2PDsJLX030190-at-ns.microscopy.com}
30, 25 -- Subject: Re: [Microscopy] viaWWW: Technique for Preparation of Polymer Film Cross
30, 25 -- Sections
30, 25 -- To: wendy.cheng-at-ipaper.com, Microscopy-at-microscopy.com
30, 25 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
30, 25 -- Message-ID: {OF37566D8A.EAF18CE1-ON85257584.004E8199-85257584.004E9FEE-at-lincolnelectric.com}
30, 25 -- Date: Wed, 25 Mar 2009 10:18:49 -0400
30, 25 -- From: Frank_Karl-at-lincolnelectric.com
30, 25 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM,
30, 25 -- CD-MIME complete at 03/25/2009 10:18:47 AM,
30, 25 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM,
30, 25 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM,
30, 25 -- Serialize complete at 03/25/2009 10:18:47 AM
30, 25 -- MIME-Version: 1.0
30, 25 -- Content-Type: text/plain;
30, 25 -- charset="UTF-8"
30, 25 -- Content-Transfer-Encoding: 8bit
30, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2PEJ55k008985
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 25 Mar 2009 10:35:17 -0500
Subject: [Microscopy] RE: Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John and Listers,
Wanted to add two comments to discussion of retaining and identifying
glycogen in tissue.

1) Several years ago there was a thread on the list about glycogen with
primary input from Krystyna Rybicka. She maintained that the protein
component associated with glycogen was subsequently washed out in
dehydration if tissue was subjected to a pH change (ie. en bloc staining
with UA) during processing. The remaining free-floating glycogen could then
clump in the cell. She recommended avoiding that pH change during
processing to retain the classic rosette structure. Once embedded, the PAS
technique could be used for detection of glycogen on the LM level and the
Thiery technique (which I think Wolfgang Muss mentioned) could be used for
EM.

Here are two references:
a) K.K. Rybicka. 1996. Tissue & Cell 28 (3) 253-267.
b) Microscopy Today, October 1994. "Glycogen Granules Revisited".

2) Many years ago when I worked at the Dana-Farber Cancer Institute we
found that the following protocol was very useful in preserving glycogen
rosettes in liver biopsies of pediatric cancer patients. It involved fixing
the tissue with osmium potassium ferrocyanide. The recipe was:

2 mls. 2% aqueous Osmium
2 mls. buffer (ie. 0.1M Na-phosphate, pH 7.4)
0.06gms potassium ferrocyanide

The OPF solution was prepared with thorough mixing just prior to use and
administered to tissue for two hours in refrigerator. The general
processing protocol was glut. and OPF fixations, alcohol and propylene oxide
dehydrations, and epon embedment. We post-stained with UA and Reynolds lead
citrate. UA enbloc staining is not recommended.

So I guess there are two important issues: preservation of glycogen in
the protein-bound form and post-embedding identification by the Thiery
technique, for example. I would think that the observance of rosettes is
strong enough evidence. Don

Donald Gantz
Research Histologist/Electron Microscopist
Dept. Physiology and Biophysics
Boston University School of Medicine
email: gantz-at-bu.edu
phone: 617-638-4017




----- Original Message -----
X-from: {john.brealey-at-imvs.sa.gov.au}
To: {gantz-at-bu.edu}
Sent: Tuesday, March 24, 2009 12:53 AM

Nicola,
My experience with mineralizing cell cultures in ESEM is not really good. Observation of cells without fixation is not possible (vaporizing of buffer leaves a lot of crystals on surfaces of specimens). Fixed cultures could be washed in distilled water. To keep cells wet they should be cooled to temperatures 2-5 degrees Celsius and ESEM should be operated at pressures above 5 torr (to be close to a dew point). A bit above dew point - and cells will be covered with a layer of water, a bit below dew point - and cells will be dehydrated rapidly (they are tiny). Since balancing exactly at dew point is practically impossible, we are working in ESEM with slowly or rapidly (as for cell cultures) dehydrating specimens. So, what we can really see in ESEM, working with cell cultures, are fixed cells, dehydrated in a microscope chamber. May be epithelium cells can resist dehydration for a while, but I did not have experience with them.

Speaking about other specimens of bigger volume/surface ratio I try to work fast, or, if necessary, I periodically go above dew point (changing pressure) to rehydrate specimens. In my experience with field-emission XL30, going below 5 kV makes signal too noisy, so I use voltages from 5 kV to 15 kV (mostly 10 kV).

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} Hi
} I have frequently imaged wet samples at 5kV and image quality
} can be variable depending on the sample. Very flat well
} attached cells on glass are some of the more problematic. But
} using higher kV means you hardly see the cells.
} However just yesterday I looked at some confluent layers of
} mineralising cells on thermanox at 5kV and 4-5 torr with
} little difficulty (but it is a FEG) Always ask researchers
} for extra specimens if possible to play with and optimise
} conditions. I routinely start low and work up to higher kV if
} necessary.
}
} ------------
}
} Tungsten gun ESEM
} In a recent review someone said ESEM was like SEM but with
} extra difficulty. Not so much difficult as frustrating when
} viewing wet biological samples. Not being gold coated they
} give off fewer secondary electrons. At lower voltages the
} signal to noise ratio makes even modest magnifications hard
} to achieve. So, I confess, I have gone from being a 5kV high
} vacuum user to a 20kV wet ESEM user.
}
} FEGESEM
} Still problematic - Kirk et al. (2009)* in an excellent
} review advocate 5-7kV.
}
} Dave
}
} * Kirk SE, Skepper JN Donald AM. 2009 Application of
} environmental scanning electron microscopy to determine
} surface structure. J Microsc 233:205-244.
}
}
} -----Original Message-----
} X-from: jacques.faerber-at-ipcms.u-strasbg.fr
} [mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
} Sent: 24 March 2009 08:39
} To: David Patton
} Subject: [Microscopy] Quest. about the ESEM
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi all
}
} In spite of beeing interesting by the concept of the ESEM
} since long ago, I had until now nor much time neither the
} real need to work on one.
} So I've a couple of questions about it.
}
} 1- First, most pictures done by ESEM in wet mode I've seen
} from collegues, either biologists or from material science,
} are done at high energy, 20-30 kV. It seems that the low
} energy "culture" is a bit ignored. (On the other hand, I
} agree that I'm perheps a bit addict to the low kV range !)
} But apart the subjecives aspects like "traditions", lab
} "culture" or the need of EDS, is there a more objective and
} technical reason for such choice ? Do the wet conditions
} limit so drastically the performences of the ESEM at low
} energy (lower then 5 keV
} f.ex.) ?
}
} 2- I a paper published in M&M 6-2000, Danilatos describes a
} very interesting evolution of the PLA (in particular for
} people interested in vaccum technology, like me...), called
} Reverse Flow Pressure Limiting Apperture. An annular gas jet
} flows from an intermediate stage at higher pressure into the
} specimen chamber, generating a pumping effect in the central
} part of the PLA and allowding to work with a greater
} differential pressure between the chamber and the column.
} I asked me if such a device has been soon integrated in
} commercial VP-SEMs, from FEI or other manufactuers. Danilatos
} mention too a cooperation with Zeiss on his website, but I
} never heard about a developpment like that by Zeiss.
}
} Thanks for comments
}
} Jacques
}
} --
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces) 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
} This message has been checked for viruses but the contents of
} an attachment may still contain software viruses, which could
} damage your computer system:
} you are advised to perform your own checks. Email
} communications with the University of Nottingham may be
} monitored as permitted by UK legislation.
}
}
}
} ==============================Original
} Headers==============================
} 23, 32 -- From Nicola.Weston-at-nottingham.ac.uk Wed Mar 25
} 08:19:36 2009 23, 32 -- Received: from smtp2.nottingham.ac.uk
} (smtp2.nottingham.ac.uk [128.243.44.5])
} 23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2PDJY0l003066
} 23, 32 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar
} 2009 08:19:35 -0500
} 23, 32 -- Received: from suismtp2.ad.nottingham.ac.uk
} ([128.243.42.11])
} 23, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
} 23, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
} 23, 32 -- id 1LmT0E-0003PW-4O
} 23, 32 -- for microscopy-at-microscopy.com; Wed, 25 Mar 2009
} 13:18:42 +0000
} 23, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk
} ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with
} Microsoft SMTPSVC(6.0.3790.3959);
} 23, 32 -- Wed, 25 Mar 2009 13:18:40 +0000
} 23, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 23,
} 32 -- Content-class: urn:content-classes:message 23, 32 --
} MIME-Version: 1.0 23, 32 -- Content-Type: text/plain;
} 23, 32 -- charset="iso-8859-1"
} 23, 32 -- Subject: Quest. about the ESEM 23, 32 -- Date: Wed,
} 25 Mar 2009 13:18:40 -0000 23, 32 -- Message-ID:
} {1E0CCC81FDE82D4C8DDE1A8F44080D940120F1E2-at-VUIEXCHA.ad.nottingh
am.ac.uk}
} 23, 32 -- X-MS-Has-Attach:
} 23, 32 -- X-MS-TNEF-Correlator:
} 23, 32 -- Thread-Topic: Quest. about the ESEM 23, 32 --
} Thread-Index: AcmtTDaSAkqv7pZtTqqlHCQUXxTF+w== 23, 32 --
} From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 23, 32
} -- To: {microscopy-at-microscopy.com} 23, 32 --
} X-OriginalArrivalTime: 25 Mar 2009 13:18:40.0992 (UTC)
} FILETIME=[37139600:01C9AD4C] 23, 32 --
} X-UoN-MailScanner-Information: Please contact
} staff-it-helpline-at-nottingham.ac.uk for more information 23,
} 32 -- X-UoN-MailScanner: Found to be clean 23, 32 --
} X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 23, 32
} -- X-Spam-Status: No 23, 32 -- Content-Transfer-Encoding:
} 8bit 23, 32 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n2PDJY0l003066
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 10:35:17 2009
7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PFZGfp031079
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 10:35:16 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Wed, 25 Mar 2009 10:35:13 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="iso-8859-1"
7, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM
7, 25 -- Date: Wed, 25 Mar 2009 10:35:12 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7FF-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200903251321.n2PDL6Np003963-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM
7, 25 -- Thread-Index: AcmtTJC11vW3PaRbQ0qkZV9ko3/MCgAEml0g
7, 25 -- References: {200903251321.n2PDL6Np003963-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 25 Mar 2009 15:35:13.0395 (UTC) FILETIME=[4A211430:01C9AD5F]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PFZGfp031079
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 25 Mar 2009 11:04:27 -0500
Subject: [Microscopy] RE: Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jacques,

I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on.

As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} Hi all
}
} In spite of beeing interesting by the concept of the ESEM
} since long ago, I had until now nor much time neither the
} real need to work on one.
} So I've a couple of questions about it.
}
} 1- First, most pictures done by ESEM in wet mode I've seen
} from collegues, either biologists or from material science,
} are done at high energy, 20-30 kV. It seems that the low
} energy "culture" is a bit ignored. (On the other hand, I
} agree that I'm perheps a bit addict to the low kV range !)
} But apart the subjecives aspects like "traditions", lab
} "culture" or the need of EDS, is there a more objective and
} technical reason for such choice ? Do the wet conditions
} limit so drastically the performences of the ESEM at low
} energy (lower then 5 keV
} f.ex.) ?
}
} 2- I a paper published in M&M 6-2000, Danilatos describes a
} very interesting evolution of the PLA (in particular for
} people interested in vaccum technology, like me...), called
} Reverse Flow Pressure Limiting Apperture. An annular gas jet
} flows from an intermediate stage at higher pressure into the
} specimen chamber, generating a pumping effect in the central
} part of the PLA and allowding to work with a greater
} differential pressure between the chamber and the column.
} I asked me if such a device has been soon integrated in
} commercial VP-SEMs, from FEI or other manufactuers. Danilatos
} mention too a cooperation with Zeiss on his website, but I
} never heard about a developpment like that by Zeiss.
}
} Thanks for comments
}
} Jacques
}
} --
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces) 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
}
} ==============================Original
} Headers==============================
} 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24
} 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr
} (mailhost.u-strasbg.fr [130.79.200.157])
} 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2O8ZMax011825
} 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar
} 2009 03:35:23 -0500
} 9, 29 -- Received: from ipcms.u-strasbg.fr
} (ipcms.u-strasbg.fr [130.79.210.2])
} 9, 29 -- by mailhost.u-strasbg.fr
} (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
} 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24
} Mar 2009 09:35:21 +0100 (CET)
} 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr
} [130.79.152.3])
} 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA
} (256/256 bits))
} 9, 29 -- (No client certificate requested)
} 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id
} 5F52D3EC002
} 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar
} 2009 09:34:14 +0100 (CET)
} 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
} 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From:
} "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
} 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9,
} 29 -- MIME-Version: 1.0 9, 29 -- To:
} microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain;
} charset=ISO-8859-1; format=flowed 9, 29 --
} Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner:
} Found to be clean 9, 29 -- X-IPCMS-MailScanner-From:
} jacques.faerber-at-ipcms.u-strasbg.fr
} 9, 29 -- Subject: Quest. about the ESEM
} 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by
} milter-greylist-4.0.1 (mailhost.u-strasbg.fr
} [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9,
} 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32
} 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29
} -- X-Spam-Status: No, score=-100.0 required=5.0
} tests=USER_IN_WHITELIST
} 9, 29 -- autolearn=disabled version=3.2.5
} 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5
} (2008-06-10) on mr7.u-strasbg.fr
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009
8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286
8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="iso-8859-1"
8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM
8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM
8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA
8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 25 Mar 2009 12:05:43 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Vladimir

I agree fully with your comment. I said "addict" as a joke, as in our
needs I'm so often in situation where I say to my collegues that we can
try with higher kV, but that I "feel" that it will give nothing. And so
it is, we try and finish between 1-5 keV. So I ask me myself sometimes
if it's an "a priori" or a addiction ! While I say always to the
students that they must try, to find the right energy for the right
illustration of their observations.

But... in the daily work, our samples are most thin metallic films,
oxyde nanoparticules, C nanotubes, etc, with the need of high
magnifications. No mineralogy any more, nor metallography, only small
thin light stuff which gives in most cases no usable pictures at 15 keV
or more.

Jacques

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



DusevichV-at-umkc.edu a écrit :
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Jacques,
}
} I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on.
}
} As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
} } Hi all
} }
} } In spite of beeing interesting by the concept of the ESEM
} } since long ago, I had until now nor much time neither the
} } real need to work on one.
} } So I've a couple of questions about it.
} }
} } 1- First, most pictures done by ESEM in wet mode I've seen
} } from collegues, either biologists or from material science,
} } are done at high energy, 20-30 kV. It seems that the low
} } energy "culture" is a bit ignored. (On the other hand, I
} } agree that I'm perheps a bit addict to the low kV range !)
} } But apart the subjecives aspects like "traditions", lab
} } "culture" or the need of EDS, is there a more objective and
} } technical reason for such choice ? Do the wet conditions
} } limit so drastically the performences of the ESEM at low
} } energy (lower then 5 keV
} } f.ex.) ?
} }
} } 2- I a paper published in M&M 6-2000, Danilatos describes a
} } very interesting evolution of the PLA (in particular for
} } people interested in vaccum technology, like me...), called
} } Reverse Flow Pressure Limiting Apperture. An annular gas jet
} } flows from an intermediate stage at higher pressure into the
} } specimen chamber, generating a pumping effect in the central
} } part of the PLA and allowding to work with a greater
} } differential pressure between the chamber and the column.
} } I asked me if such a device has been soon integrated in
} } commercial VP-SEMs, from FEI or other manufactuers. Danilatos
} } mention too a cooperation with Zeiss on his website, but I
} } never heard about a developpment like that by Zeiss.
} }
} } Thanks for comments
} }
} } Jacques
} }
} } --
} } J. Faerber
} } IPCMS-GSI
} } (Institut de Physique et Chimie des Matériaux de Strasbourg
} } Groupe Surface et Interfaces) 23, rue de Loess ; BP43
} } 67034 Strasbourg CEDEX 2
} } France
} }
} } Tel 00 33(0)3 88 10 71 01
} } Fax 00 33(0)3 88 10 72 48
} } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24
} } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr
} } (mailhost.u-strasbg.fr [130.79.200.157])
} } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n2O8ZMax011825
} } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar
} } 2009 03:35:23 -0500
} } 9, 29 -- Received: from ipcms.u-strasbg.fr
} } (ipcms.u-strasbg.fr [130.79.210.2])
} } 9, 29 -- by mailhost.u-strasbg.fr
} } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
} } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24
} } Mar 2009 09:35:21 +0100 (CET)
} } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr
} } [130.79.152.3])
} } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA
} } (256/256 bits))
} } 9, 29 -- (No client certificate requested)
} } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id
} } 5F52D3EC002
} } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar
} } 2009 09:34:14 +0100 (CET)
} } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
} } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From:
} } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
} } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9,
} } 29 -- MIME-Version: 1.0 9, 29 -- To:
} } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain;
} } charset=ISO-8859-1; format=flowed 9, 29 --
} } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner:
} } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From:
} } jacques.faerber-at-ipcms.u-strasbg.fr
} } 9, 29 -- Subject: Quest. about the ESEM
} } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by
} } milter-greylist-4.0.1 (mailhost.u-strasbg.fr
} } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9,
} } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32
} } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29
} } -- X-Spam-Status: No, score=-100.0 required=5.0
} } tests=USER_IN_WHITELIST
} } 9, 29 -- autolearn=disabled version=3.2.5
} } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5
} } (2008-06-10) on mr7.u-strasbg.fr
} } ==============================End of -
} } Headers==============================
} }
} }
} }
}
}
} ==============================Original Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009
} 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500
} 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="iso-8859-1"
} 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM
} 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500
} 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM
} 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA
} 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009
9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PH5fou009666
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41 -0500
9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2PH5ct7066933
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 18:05:38 +0100 (CET)
9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 31 -- (No client certificate requested)
9, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001
9, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25 +0100 (CET)
9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr}
9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100
9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
9, 31 -- MIME-Version: 1.0
9, 31 -- To: Microscopy-at-microscopy.com
9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM
9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-IPCMS-MailScanner: Found to be clean
9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar 2009 18:05:38 +0100 (CET)
9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on mr2.u-strasbg.fr
9, 31 -- X-Virus-Status: Clean
9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
9, 31 -- autolearn=disabled version=3.2.5
9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr2.u-strasbg.fr
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Wed, 25 Mar 2009 12:06:02 -0500
Subject: [Microscopy] viaWWW: Technique for Preparation of Polymer Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

If you are working in cryo mode inject the solution into a narrow drinking
straw about 1/2 inch tall that is mounted in your cryo holder. Freeze the
unit and then crack it open in your cryo manipulation chamber. I used glass
rods for this in the early days of cryo but found that the very narrow
drinking straws are far better. The best are those that are often used as
stirrers for do it yourself coffee making!

I think we are in the same area as a few weeks ago when we were talking
about looking at cross sections but only if you could crack them in air?

If the material will solidify in air cast it onto a piece of aluminium foil.
Once dry place the foil in liquid nitrogen and then collect the media as it
cracks off the foil. The different contraction rates of foil and media will
cause the media to crack into nice cross sections.

If the material is quite stiff or may be mounted firmly on a stiff surface
cut it down to about 1 1/4inch by 3/8 inch. Place it in liquid nitrogen
until the solution stops bubbling. Remove the specimen and bend it until it
cracks. If it will not crack neck the material at the half way point and
try again. For more details and diagrams look at
www.emcourses.com/crack.htm

When a material will not fracture naturally and you are unable to use a cryo
device you need to stiffen the material. If the material is not soluble in
water we use a water soluble carbon solution as the stiffener for example
Agar G303. Drill a fine (1/8th inch) hole though two stubs placed face to
face. Place your fibres/material through the hole and fill the additional
space with the carbon solution. When dry plunge the unit into liquid
nitrogen and when the liquid stops boiling take the unit out and crack it by
striking the interface with a single edged blade. Take great care not to
cut through the nit, simple crack it open.

Once the two units are back at room temperature and the condensation
has dispersed they are usable in the light microscope and the SEM.

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com

-----Original Message-----
X-from: wendy.cheng-at-ipaper.com [mailto:wendy.cheng-at-ipaper.com]
Sent: 25 March 2009 13:47
To: protrain-at-emcourses.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wendy.cheng-at-ipaper.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: wendy.cheng-at-ipaper.com
Name: Wendy Cheng

Organization: International Paper

Title-Subject: [Filtered] Technique for Preparation of Polymer Film
Cross Sections

Question: Does anyone have a good technique for preparation of (cryo)
cross sections of polymer films for SEM examination? I am interested
in viewing the distribution of two polymers in a polymer blend.

Wendy Cheng, Ph.D.
International Paper
6283 Tri-Ridge Blvd.
Loveland, OH 45140

Phone: 513-248-6698
email: wendy.cheng-at-ipaper.com

Login Host: 141.129.1.98
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 08:45:39 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2PDjbTk017677
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 08:45:38
-0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p0624080dc5efe5f4bc62-at-[206.69.208.22]}
8, 11 -- Date: Wed, 25 Mar 2009 08:45:36 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: wendy.cheng-at-ipaper.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Technique for Preparation of Polymer Film Cross
Sections
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
22, 27 -- From protrain-at-emcourses.com Wed Mar 25 12:06:01 2009
22, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PH5xBc009853
22, 27 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:06:00 -0500
22, 27 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:1096 helo=HP6220)
22, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
22, 27 -- (envelope-from {protrain-at-emcourses.com} )
22, 27 -- id 1LmWY7-000NAT-DQ by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 19:05:55 +0200
22, 27 -- Reply-To: {protrain-at-emcourses.com}
22, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com}
22, 27 -- To: {wendy.cheng-at-ipaper.com}
22, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
22, 27 -- References: {200903251347.n2PDl6aY019129-at-ns.microscopy.com}
22, 27 -- Subject: RE: [Microscopy] viaWWW: Technique for Preparation of Polymer Film Cross Sections
22, 27 -- Date: Wed, 25 Mar 2009 17:05:31 -0000
22, 27 -- Organization: Protrain
22, 27 -- Message-ID: {004d01c9ad6b$f671d150$0200a8c0-at-HP6220}
22, 27 -- MIME-Version: 1.0
22, 27 -- Content-Type: text/plain;
22, 27 -- charset="us-ascii"
22, 27 -- Content-Transfer-Encoding: 7bit
22, 27 -- X-Mailer: Microsoft Office Outlook 11
22, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
22, 27 -- Thread-Index: AcmtUECg2tLJeRZjQYyAbqjyp1oIHwAF3bAg
22, 27 -- In-Reply-To: {200903251347.n2PDl6aY019129-at-ns.microscopy.com}
22, 27 -- X-Scan-Signature: 761d6cf5b62eb846ccb869db0bc84167{107}}
22, 27 -- X-Trace: smtp01.dial-up.net 1LmWY7-000NAT-DQ 445726e93845c6a89f9b67ae442bbbe6
==============================End of - Headers==============================




From: rbeavers-at-mail.smu.edu
Date: Wed, 25 Mar 2009 12:58:50 -0500
Subject: [Microscopy] Translation based stereo pair SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Microscopy Society of the Ohio River Valley (MSORV) announces their
upcoming Spring Meeting.


Group,

Do any of you have a few translation based (vs eucentric tilting) stereo pair SEM images you could share?

I am evaluating some software to extract geometric information from these types of images.

If you can help it would be greatly appreciated.

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
7, 26 -- From rbeavers-at-mail.smu.edu Wed Mar 25 12:58:50 2009
7, 26 -- Received: from sxet1p2.systems.smu.edu (sxet1p2.systems.smu.edu [129.119.65.147])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PHwnO0019930
7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:58:49 -0500
7, 26 -- Received: from sxht1p2.systems.smu.edu (129.119.65.133) by
7, 26 -- sxet1p2.systems.smu.edu (129.119.65.147) with Microsoft SMTP Server (TLS) id
7, 26 -- 8.1.340.0; Wed, 25 Mar 2009 12:58:48 -0500
7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
7, 26 -- sxht1p2.systems.smu.edu ([129.119.65.133]) with mapi; Wed, 25 Mar 2009
7, 26 -- 12:58:40 -0500
7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 26 -- Date: Wed, 25 Mar 2009 12:58:40 -0500
7, 26 -- Subject: Translation based stereo pair SEM images
7, 26 -- Thread-Topic: Translation based stereo pair SEM images
7, 26 -- Thread-Index: Acmtc1RmxJZ2cgzYTBOklF23fmzjwg==
7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB41C22DB037-at-SXMBXC.systems.smu.edu}
7, 26 -- Accept-Language: en-US
7, 26 -- Content-Language: en-US
7, 26 -- X-MS-Has-Attach:
7, 26 -- X-MS-TNEF-Correlator:
7, 26 -- acceptlanguage: en-US
7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
7, 26 -- MIME-Version: 1.0
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PHwnO0019930
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Wed, 25 Mar 2009 13:02:46 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All

Taking up the comments about(E)SEM accelerating voltage and how it should be
used I am often tempted to ask if a laboratory runs by tradition or by
science, unfortunately too many run by tradition!

In my mind the most important question an SEM operator has to answer is
"Which kV?" The correct answer in my mind is "The kV that displays the most
information about the specimen." However I often find that to obtain the
most information about a specimen it is good to view it at higher and lower
accelerating voltages - my choice 2 & 10 or 5 and 15.

The only time I have my students use the highest accelerating voltages are
when using BSE techniques. We have worked as low as 100 volts on a tungsten
hairpin instrument, a 15 year old model in fact!

The motto "Microscopists are scientists they should experiment."

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com

-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr
[mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: 25 March 2009 17:08
To: protrain-at-emcourses.com

Hi Vladimir

I agree fully with your comment. I said "addict" as a joke, as in our
needs I'm so often in situation where I say to my collegues that we can
try with higher kV, but that I "feel" that it will give nothing. And so
it is, we try and finish between 1-5 keV. So I ask me myself sometimes
if it's an "a priori" or a addiction ! While I say always to the
students that they must try, to find the right energy for the right
illustration of their observations.

But... in the daily work, our samples are most thin metallic films,
oxyde nanoparticules, C nanotubes, etc, with the need of high
magnifications. No mineralogy any more, nor metallography, only small
thin light stuff which gives in most cases no usable pictures at 15 keV
or more.

Jacques

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



DusevichV-at-umkc.edu a écrit :
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Jacques,
}
} I cannot understand "addiction" to any kV range. In my work I routinely
use all voltages my microscope can supply: from 300 V for easily damaged or
charging specimens to 30 kV for "special effects". For example, at 30 kV I
can get pictures of yeas colonies with white cells on black background
(useful for analysis of shapes of colonies). At 15 kV I can make pictures
with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20
kV could be useful in imaging fractures of multiphase specimens like
composites and metals, when phases are highlighted. And so on.
}
} As for ESEM, low voltages ( {5kV for my microscope) are not really useful
because of high noise level.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
} } Hi all
} }
} } In spite of beeing interesting by the concept of the ESEM
} } since long ago, I had until now nor much time neither the
} } real need to work on one.
} } So I've a couple of questions about it.
} }
} } 1- First, most pictures done by ESEM in wet mode I've seen
} } from collegues, either biologists or from material science,
} } are done at high energy, 20-30 kV. It seems that the low
} } energy "culture" is a bit ignored. (On the other hand, I
} } agree that I'm perheps a bit addict to the low kV range !)
} } But apart the subjecives aspects like "traditions", lab
} } "culture" or the need of EDS, is there a more objective and
} } technical reason for such choice ? Do the wet conditions
} } limit so drastically the performences of the ESEM at low
} } energy (lower then 5 keV
} } f.ex.) ?
} }
} } 2- I a paper published in M&M 6-2000, Danilatos describes a
} } very interesting evolution of the PLA (in particular for
} } people interested in vaccum technology, like me...), called
} } Reverse Flow Pressure Limiting Apperture. An annular gas jet
} } flows from an intermediate stage at higher pressure into the
} } specimen chamber, generating a pumping effect in the central
} } part of the PLA and allowding to work with a greater
} } differential pressure between the chamber and the column.
} } I asked me if such a device has been soon integrated in
} } commercial VP-SEMs, from FEI or other manufactuers. Danilatos
} } mention too a cooperation with Zeiss on his website, but I
} } never heard about a developpment like that by Zeiss.
} }
} } Thanks for comments
} }
} } Jacques
} }
} } --
} } J. Faerber
} } IPCMS-GSI
} } (Institut de Physique et Chimie des Matériaux de Strasbourg
} } Groupe Surface et Interfaces) 23, rue de Loess ; BP43
} } 67034 Strasbourg CEDEX 2
} } France
} }
} } Tel 00 33(0)3 88 10 71 01
} } Fax 00 33(0)3 88 10 72 48
} } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24
} } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr
} } (mailhost.u-strasbg.fr [130.79.200.157])
} } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n2O8ZMax011825
} } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar
} } 2009 03:35:23 -0500
} } 9, 29 -- Received: from ipcms.u-strasbg.fr
} } (ipcms.u-strasbg.fr [130.79.210.2])
} } 9, 29 -- by mailhost.u-strasbg.fr
} } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
} } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24
} } Mar 2009 09:35:21 +0100 (CET)
} } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr
} } [130.79.152.3])
} } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA
} } (256/256 bits))
} } 9, 29 -- (No client certificate requested)
} } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id
} } 5F52D3EC002
} } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar
} } 2009 09:34:14 +0100 (CET)
} } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
} } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From:
} } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
} } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9,
} } 29 -- MIME-Version: 1.0 9, 29 -- To:
} } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain;
} } charset=ISO-8859-1; format=flowed 9, 29 --
} } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner:
} } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From:
} } jacques.faerber-at-ipcms.u-strasbg.fr
} } 9, 29 -- Subject: Quest. about the ESEM
} } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by
} } milter-greylist-4.0.1 (mailhost.u-strasbg.fr
} } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9,
} } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32
} } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29
} } -- X-Spam-Status: No, score=-100.0 required=5.0
} } tests=USER_IN_WHITELIST
} } 9, 29 -- autolearn=disabled version=3.2.5
} } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5
} } (2008-06-10) on mr7.u-strasbg.fr
} } ==============================End of -
} } Headers==============================
} }
} }
} }
}
}
} ==============================Original
Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009
} 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu
[134.193.143.155])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2PG4PlI026286
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26
-0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500
} 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="iso-8859-1"
} 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM
} 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500
} 8, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM
} 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA
} 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC)
FILETIME=[5D87D3C0:01C9AD63]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2PG4PlI026286
} ==============================End of -
Headers==============================
}

==============================Original Headers==============================
9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009
9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr
[130.79.200.152])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2PH5fou009666
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41
-0500
9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr
[130.79.210.2])
9, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with
ESMTP id n2PH5ct7066933
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009
18:05:38 +0100 (CET)
9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 31 -- (No client certificate requested)
9, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001
9, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25
+0100 (CET)
9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr}
9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100
9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
9, 31 -- MIME-Version: 1.0
9, 31 -- To: Microscopy-at-microscopy.com
9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM
9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-IPCMS-MailScanner: Found to be clean
9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by
milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar
2009 18:05:38 +0100 (CET)
9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on
mr2.u-strasbg.fr
9, 31 -- X-Virus-Status: Clean
9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0
tests=USER_IN_WHITELIST
9, 31 -- autolearn=disabled version=3.2.5
9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on
mr2.u-strasbg.fr
==============================End of - Headers==============================



==============================Original Headers==============================
22, 28 -- From protrain-at-emcourses.com Wed Mar 25 13:02:46 2009
22, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PI2h8V027035
22, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 13:02:44 -0500
22, 28 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:4885 helo=HP6220)
22, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
22, 28 -- (envelope-from {protrain-at-emcourses.com} )
22, 28 -- id 1LmXQz-000OBX-Jj by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 20:02:38 +0200
22, 28 -- Reply-To: {protrain-at-emcourses.com}
22, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com}
22, 28 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr}
22, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
22, 28 -- References: {200903251708.n2PH80VM012463-at-ns.microscopy.com}
22, 28 -- Subject: Tradition or Science
22, 28 -- Date: Wed, 25 Mar 2009 18:02:36 -0000
22, 28 -- Organization: Protrain
22, 28 -- Message-ID: {001b01c9ad73$e2937a50$0200a8c0-at-HP6220}
22, 28 -- MIME-Version: 1.0
22, 28 -- Content-Type: text/plain;
22, 28 -- charset="iso-8859-1"
22, 28 -- X-Mailer: Microsoft Office Outlook 11
22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
22, 28 -- Thread-Index: AcmtbFDGBlAaAMq6QsSK8oTs72K/dgABixYg
22, 28 -- In-Reply-To: {200903251708.n2PH80VM012463-at-ns.microscopy.com}
22, 28 -- X-Scan-Signature: 99f4c94f445b5fbf97b5591e34ffbdcf{337}}
22, 28 -- X-Trace: smtp01.dial-up.net 1LmXQz-000OBX-Jj d3c9f4506833b893560218b1f3ba629a
22, 28 -- Content-Transfer-Encoding: 8bit
22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PI2h8V027035
==============================End of - Headers==============================




From: yvan_lindekens-at-yahoo.com
Date: Thu, 26 Mar 2009 01:17:38 -0500
Subject: [Microscopy] LM: Homebrew programmable slide stainer: opinions and suggestions wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I'm thinking on building a programmable slide stainer, starting from a liniar one. I own a Medite COT20 liniar stainer (see the leaflet -at- http://tinyurl.com/cgtxo5 or http://preview.tinyurl.com/cgtxo5). It's in working condition. It has no less than 27 stations, so it can be used to perform even rather demanding staining protocols such as those often used trichromes as AZAN, Van Gieson, Masson etc.

The working principle of liniar slide stainers is, that the slides are run trough the baths and the time in each bath is the same for all baths. That way it's possible to run basket after basket after basket... trough the machine, resulting in very high troughput numbers (the COT 20 should be capable of staining up to 1 000 slides/hour, according to Medite). Variation is done by altering the concentration of the solutions used or by putting several baths after each other.

An example. Let's say staining by hand:
---------------------------------------
...
hematoxylin: 6 min
differentiation 1% ²HCl in ETOH 70: 15 sec
water: 2 min
bluing: 2 min
...

would become something like this, using a liniar slide stainer:
--------------------------------------------------------------
...
hematoxylin 1: 2 min
hematoxylin 2: 2 min
hematoxylin 3: 2 min
differentiation 0.1% ²HCl in ETOH 70: 2 min
water: 2 min
bluing: 2 min
...

But protocols such as this example are impossible in a liniar slide stainer:
----------------------------------------------------------------------------

...
mordanting 0.1% chromium trioxide in dH2O: 5h
running water: 5h
safranin 1% in ETOH 50: 20 min
ETOH 50: 10 sec
...

For those you need a programmable slide stainer. The running water isn't the problem, it's the variation in treatment times.


As I also have a few old PC's gathering dust on my attic (486/33, 486/100, PI/166, PII/400...), I would like to use one of those to control the stainer using good ol' DOS batch files.
I would prefer the 486/33 as it is a compact model with an integrated screen (one of those old Compaq Presario cubes).

The general idea is to control the stainer using an external relay board and a relay controller board in the computer. Given the age of the PC's I'm looking for a second hand ISA (prefeberaly) or a PCI controller board.

Controling the stainer shouldn't be that hard as about the only thing needed is a programmable timing cycle, a square wave with in time adjustable 1's and 0's. Or am I missing something? I've noticed already that it's rather simple to set up a timing cycle in NTFS-DOS by using ping 127.0.0.1 -n# but I suppose it isn't usable in DOS 6.**.

In the next couple of days I will dismantle the Cot 20 and draw it's wiring, but it looks like it's rather simple: the only thing needed is to control one cycle of a stepping motor in the machine. It's currently controlled by an Omron timer. There's no need to controll the other controls on the machine (draining time controlled by another Omron timer and agitation on/off) trough the PC.

Any thoughts?

Thanks in advance!

Y.





==============================Original Headers==============================
20, 22 -- From yvan_lindekens-at-yahoo.com Thu Mar 26 01:17:38 2009
20, 22 -- Received: from web110213.mail.gq1.yahoo.com (web110213.mail.gq1.yahoo.com [67.195.8.189])
20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2Q6Hb7H006374
20, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 01:17:38 -0500
20, 22 -- Received: (qmail 88331 invoked by uid 60001); 26 Mar 2009 06:17:37 -0000
20, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238048257; bh=gBv/dIta4B/ojemlpbOVoQ2TxNPOIGs823zLvT+ZKZI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=1lrWCXfmjFIcJQ1lATl+M7hJWPp5L+WnRjIoumAX/GirM3Hy2xQQC2HEIWvOPLcHfrXw0TuWYBiwAhFERuDTsT7htYwuexDOUQRvwS7LDEQiNO57gjqAh1p9Zso8WEqGyohiss7oVCkwAZ2OGuLWUleAk+5lLL8qehvg0y5U6Pw=
20, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
20, 22 -- s=s1024; d=yahoo.com;
20, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
20, 22 -- b=qZsyJZ12r+oaEgQPtSQ6l+lBU+SxauIyAI2ydmE8aPfD/s5y3l6zD1zh+pfxAhLKnSpAHUDFhExhmsRtaXMUhlNC4CxDIU294cpdW/n8RmxRPU5BxxZBXVzKxWu8AoXubE3KwMD/ob6wQCLIV/vZr5mP0wONIInB7t8734Zekpc=;
20, 22 -- Message-ID: {357798.87844.qm-at-web110213.mail.gq1.yahoo.com}
20, 22 -- X-YMail-OSG: HebBYGYVM1mr.f5zmvt5t8DV7OeBFw9hiwqP4ltsdyWk5XTV1olxls.y
20, 22 -- Received: from [87.64.45.30] by web110213.mail.gq1.yahoo.com via HTTP; Wed, 25 Mar 2009 23:17:36 PDT
20, 22 -- X-Mailer: YahooMailWebService/0.7.289.1
20, 22 -- Date: Wed, 25 Mar 2009 23:17:36 -0700 (PDT)
20, 22 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com}
20, 22 -- Subject: LM: Homebrew programmable slide stainer: opinions and suggestions wanted
20, 22 -- To: Microscopy-at-microscopy.com
20, 22 -- MIME-Version: 1.0
20, 22 -- Content-Type: text/plain; charset=iso-8859-1
20, 22 -- Content-Transfer-Encoding: 8bit
20, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2Q6Hb7H006374
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 26 Mar 2009 04:31:48 -0500
Subject: [Microscopy] Quest. about the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The most  information or the most meaningful information?

I really think that the vast majority of SEM users know how to use the kV and why.

Now I wonder how long it took to take a picture at 100 V. Overnight? ;-)

Stephane


----- Original Message ----
X-from: "protrain-at-emcourses.com" {protrain-at-emcourses.com}
To: nizets2-at-yahoo.com
Sent: Wednesday, March 25, 2009 7:06:31 PM

Hi All

Taking up the comments about(E)SEM accelerating voltage and how it should be
used I am often tempted to ask if a laboratory runs by tradition or by
science, unfortunately too many run by tradition!

In my mind the most important question an SEM operator has to answer is
"Which kV?"  The correct answer in my mind is "The kV that displays the most
information about the specimen." However I often find that to obtain the
most information about a specimen it is good to view it at higher and lower
accelerating voltages - my choice 2 & 10 or 5 and 15. 

The only time I have my students use the highest accelerating voltages are
when using BSE techniques.  We have worked as low as 100 volts on a tungsten
hairpin instrument, a 15 year old model in fact!

The motto "Microscopists are scientists they should experiment."

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967  www.emcourses.com

-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr
[mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: 25 March 2009 17:08
To: protrain-at-emcourses.com

Hi Vladimir

I agree fully with your comment. I said "addict" as a joke, as in our
needs I'm so often in situation where I say to my collegues that we can
try with higher kV, but that I "feel" that it will give nothing. And so
it is, we try and finish between 1-5 keV. So I ask me myself sometimes
if it's an "a priori" or a addiction ! While I say always to the
students that they must try, to find the right energy for the right
illustration of their observations.

But... in the daily work, our samples are most thin metallic films,
oxyde nanoparticules, C nanotubes, etc, with the need of high
magnifications. No mineralogy any more, nor metallography, only small
thin light stuff which gives in most cases no usable pictures at 15 keV
or more.

Jacques

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



DusevichV-at-umkc.edu a écrit :
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Jacques,
}
} I cannot understand "addiction" to any kV range. In my work I routinely
use all voltages my microscope can supply: from 300 V for easily damaged or
charging specimens to 30 kV for "special effects". For example, at 30 kV I
can get pictures of yeas colonies with white cells on black background
(useful for analysis of shapes of colonies). At 15 kV I can make pictures
with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20
kV could be useful in imaging fractures of multiphase specimens like
composites and metals, when phases are highlighted. And so on.
}
} As for ESEM, low voltages ( {5kV for my microscope) are not really useful
because of high noise level.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax:  (816) 235-5524
} Web:    http://www.umkc.edu/dentistry/microscopy
}
}  
} } Hi all
} }
} } In spite of beeing interesting by the concept of the ESEM
} } since long ago, I had until now nor much time neither the
} } real need to work on one.
} } So I've a couple of questions about it.
} }
} } 1- First, most pictures done by ESEM in wet mode I've seen
} } from collegues, either biologists or from material science,
} } are done at high energy, 20-30 kV. It seems that the low
} } energy "culture" is a bit ignored. (On the other hand, I
} } agree that I'm perheps a bit addict to the low kV range !)
} } But apart the subjecives aspects like "traditions", lab
} } "culture" or the need of EDS, is there a more objective and
} } technical reason for such choice ? Do the wet conditions
} } limit so drastically the performences of the ESEM at low
} } energy (lower then 5 keV
} } f.ex.) ?
} }
} } 2- I a paper published in M&M 6-2000, Danilatos describes a
} } very interesting evolution of the PLA (in particular for
} } people interested in vaccum technology, like me...), called
} } Reverse Flow Pressure Limiting Apperture. An annular gas jet
} } flows from an intermediate stage at higher pressure into the
} } specimen chamber, generating a pumping effect in the central
} } part of the PLA and allowding to work with a greater
} } differential pressure between the chamber and the column.
} } I asked me if such a device has been soon integrated in
} } commercial VP-SEMs, from FEI or other manufactuers. Danilatos
} } mention too a cooperation with Zeiss on his website, but I
} } never heard about a developpment like that by Zeiss.
} }
} } Thanks for comments
} }
} } Jacques
} }
} } --
} } J. Faerber
} } IPCMS-GSI
} } (Institut de Physique et Chimie des Matériaux de Strasbourg
} } Groupe Surface et Interfaces) 23, rue de Loess ; BP43
} } 67034 Strasbourg CEDEX 2
} } France
} }
} } Tel 00 33(0)3 88 10 71 01
} } Fax 00 33(0)3 88 10 72 48
} } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
} }
} }  
} } ==============================Original
} } Headers==============================
} } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24
} } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr
} } (mailhost.u-strasbg.fr [130.79.200.157])
} } 9, 29 --     by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n2O8ZMax011825
} } 9, 29 --     for {microscopy-at-microscopy.com} ; Tue, 24 Mar
} } 2009 03:35:23 -0500
} } 9, 29 -- Received: from ipcms.u-strasbg.fr
} } (ipcms.u-strasbg.fr [130.79.210.2])
} } 9, 29 --          by mailhost.u-strasbg.fr
} } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280
} } 9, 29 --          for {microscopy-at-microscopy.com} ; Tue, 24
} } Mar 2009 09:35:21 +0100 (CET)
} } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr
} } [130.79.152.3])
} } 9, 29 --     (using TLSv1 with cipher DHE-RSA-AES256-SHA
} } (256/256 bits))
} } 9, 29 --     (No client certificate requested)
} } 9, 29 --     by ipcms.u-strasbg.fr (Postfix) with ESMTP id
} } 5F52D3EC002
} } 9, 29 --     for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar
} } 2009 09:34:14 +0100 (CET)
} } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr}
} } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From:
} } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
} } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9,
} } 29 -- MIME-Version: 1.0 9, 29 -- To:
} } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain;
} } charset=ISO-8859-1; format=flowed 9, 29 --
} } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner:
} } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From:
} } jacques.faerber-at-ipcms.u-strasbg.fr
} } 9, 29 -- Subject: Quest. about the ESEM
} } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by
} } milter-greylist-4.0.1 (mailhost.u-strasbg.fr
} } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9,
} } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32
} } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29
} } -- X-Spam-Status: No, score=-100.0 required=5.0
} } tests=USER_IN_WHITELIST
} } 9, 29 --     autolearn=disabled version=3.2.5
} } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5
} } (2008-06-10) on mr7.u-strasbg.fr
} } ==============================End of -
} } Headers==============================
} }
} }
} }    
}
}  
} ==============================Original
Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009
} 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu
[134.193.143.155])
} 8, 25 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2PG4PlI026286
} 8, 25 --     for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26
-0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 --     Wed, 25 Mar 2009 11:04:23 -0500
} 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 --     charset="iso-8859-1"
} 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM
} 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500
} 8, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM
} 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA
} 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC)
FILETIME=[5D87D3C0:01C9AD63]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2PG4PlI026286
} ==============================End of -
Headers==============================
}  

==============================Original Headers==============================
9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009
9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr
[130.79.200.152])
9, 31 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2PH5fou009666
9, 31 --     for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41
-0500
9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr
[130.79.210.2])
9, 31 --          by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with
ESMTP id n2PH5ct7066933
9, 31 --          for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009
18:05:38 +0100 (CET)
9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 31 --     (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 31 --     (No client certificate requested)
9, 31 --     by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001
9, 31 --     for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25
+0100 (CET)
9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr}
9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100
9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
9, 31 -- MIME-Version: 1.0
9, 31 -- To: Microscopy-at-microscopy.com
9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM
9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com}
9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-IPCMS-MailScanner: Found to be clean
9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by
milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar
2009 18:05:38 +0100 (CET)
9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on
mr2.u-strasbg.fr
9, 31 -- X-Virus-Status: Clean
9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0
tests=USER_IN_WHITELIST
9, 31 --     autolearn=disabled version=3.2.5
9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on
mr2.u-strasbg.fr
==============================End of - Headers==============================



==============================Original Headers==============================
22, 28 -- From protrain-at-emcourses.com Wed Mar 25 13:02:46 2009
22, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
22, 28 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PI2h8V027035
22, 28 --     for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 13:02:44 -0500
22, 28 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:4885 helo=HP6220)
22, 28 --     by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
22, 28 --     (envelope-from {protrain-at-emcourses.com} )
22, 28 --     id 1LmXQz-000OBX-Jj by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 20:02:38 +0200
22, 28 -- Reply-To: {protrain-at-emcourses.com}
22, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com}
22, 28 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr}
22, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
22, 28 -- References: {200903251708.n2PH80VM012463-at-ns.microscopy.com}
22, 28 -- Subject: Tradition or Science
22, 28 -- Date: Wed, 25 Mar 2009 18:02:36 -0000
22, 28 -- Organization: Protrain
22, 28 -- Message-ID: {001b01c9ad73$e2937a50$0200a8c0-at-HP6220}
22, 28 -- MIME-Version: 1.0
22, 28 -- Content-Type: text/plain;
22, 28 --     charset="iso-8859-1"
22, 28 -- X-Mailer: Microsoft Office Outlook 11
22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
22, 28 -- Thread-Index: AcmtbFDGBlAaAMq6QsSK8oTs72K/dgABixYg
22, 28 -- In-Reply-To: {200903251708.n2PH80VM012463-at-ns.microscopy.com}
22, 28 -- X-Scan-Signature: 99f4c94f445b5fbf97b5591e34ffbdcf{337}}
22, 28 -- X-Trace: smtp01.dial-up.net 1LmXQz-000OBX-Jj d3c9f4506833b893560218b1f3ba629a
22, 28 -- Content-Transfer-Encoding: 8bit
22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PI2h8V027035
==============================End of - Headers==============================






==============================Original Headers==============================
39, 24 -- From nizets2-at-yahoo.com Thu Mar 26 04:31:48 2009
39, 24 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
39, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2Q9VlJC027468
39, 24 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 04:31:47 -0500
39, 24 -- Received: (qmail 18303 invoked by uid 60001); 26 Mar 2009 09:31:46 -0000
39, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238059906; bh=jvL67qBGgTkLlbjYpLm8dKQf9xS+u4ysbsgMqw+aalQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=u7XWzg8J/tLHafTbN1kS0p/8++IyTRkxJ7LntZ0w4V1eMZ3zbODHj3b44dOEXo3GvKfkzSb6w/JouNLFRVCXJgt7LxIOBYE9ZLJfgyGN5A3NzO3u2cwWQs+dM/6srll0enx9tOfvN5pGUVTYr+HdrW2J2Ki7qicsA4WiCEx6DuM=
39, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
39, 24 -- s=s1024; d=yahoo.com;
39, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
39, 24 -- b=3ifcXQzVU1DwEH6RW+Skso2L8z8ZutOCfLVfv9K25sRNgvoBVXa4ZXQdgq+VKBd8+yMHQ0zEZaysMexaNrW6PgB7gPsLE3psPaqIvGj4NN047vzBuiqc+SJ2dA+zzDLLmUjk8B8k1rwO9fCcHZX6daqCuRgmZWHQFBT6EfRdJ1E=;
39, 24 -- Message-ID: {219772.18074.qm-at-web110814.mail.gq1.yahoo.com}
39, 24 -- X-YMail-OSG: u0Qt7wIVM1lITpP0yGo0jlsCCEvenU4HtnelFz0uAdTuG3hFu_M2.TAZ
39, 24 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Thu, 26 Mar 2009 02:31:45 PDT
39, 24 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1
39, 24 -- References: {200903251806.n2PI6VFb006201-at-ns.microscopy.com}
39, 24 -- Date: Thu, 26 Mar 2009 02:31:45 -0700 (PDT)
39, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
39, 24 -- Subject: Re: [Microscopy] Tradition or Science
39, 24 -- To: microscopy-at-microscopy.com
39, 24 -- In-Reply-To: {200903251806.n2PI6VFb006201-at-ns.microscopy.com}
39, 24 -- MIME-Version: 1.0
39, 24 -- Content-Type: text/plain; charset=iso-8859-1
39, 24 -- Content-Transfer-Encoding: 8bit
39, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2Q9VlJC027468
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 26 Mar 2009 04:55:44 -0500
Subject: [Microscopy] viaWWW: Fibrin clots- SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"TEM micrographs are not what the student would like to present in his paper".

I am shocked.
Perhaps it would be the right moment to teach the student that his method is not a scientific one but more like a sectarian one.
A scientist doesn't choose the technique and then looks for a way to reach his goal with this technique. A scientist actually has a goal and chooses the technique(s) which is(are) the most adapted to reach his goal.

I think for this case Electrontomography could give interesting results. If he still refuses to even try that, you can still suggest that he tries politics. In politics, whatever the method, the most important thing is to reach the goal (*)

Best regards,

Stephane

(*) Please don't send me insulting emails. This was just intended to be humour. No personal harm intended. Not even to politicans. I swear. In case of offense, just replace "politics" by "professional sport".



----- Original Message ----
X-from: "dhorne-at-interchange.ubc.ca" {dhorne-at-interchange.ubc.ca}
To: nizets2-at-yahoo.com
Sent: Tuesday, March 24, 2009 6:16:46 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both dhorne-at-interchange.ubc.ca as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] Fibrin clots- SEM

Question: We have a student interested in looking at the morphologies
(fibre arrangement and spacing) of fibrin clots treated with various
stabilizing agents as a bulk sample, not as a thin layer. I have
tried a few different techniques- CPD vs HMDS, ROTO, holding the clot
between dialysis membranes in a cartridge arrangement (for the record
this was a miserable failure),and processing the clot formed in an
Eppendorf tube- but continue to have issues with collapse of the clot.

We've done a lit search but haven't been able to find a definitive
technique for a bulk clot. It is important to note this is an SEM
application, and that TEM micrographs are not what the student would
like to present in his paper.

I'm considering taking the clots to 100% EtOH and then freeze-drying,
but my gut tells me the clots will still collapse.

I am wondering if it is even possible to keep a bulk clot expanded?
Should fibrin be thought of as a cable, without strength in
compression? If so, how have people worked around this in the past?

Thanks,

Derrick Horne





  Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Tue Mar 24 12:08:47 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OH8kPV005652
15, 11 --     for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 12:08:47 -0500
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240805c5eec3fcc4e0-at-[206.69.208.22]}
15, 11 -- Date: Tue, 24 Mar 2009 12:08:46 -0500
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Fibrin clots- SEM
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
34, 25 -- From nizets2-at-yahoo.com Thu Mar 26 04:55:43 2009
34, 25 -- Received: from web110811.mail.gq1.yahoo.com (web110811.mail.gq1.yahoo.com [67.195.13.234])
34, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2Q9tfm5009347
34, 25 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 04:55:42 -0500
34, 25 -- Received: (qmail 58547 invoked by uid 60001); 26 Mar 2009 09:55:40 -0000
34, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238061340; bh=MHOEzeoTCniIWVIalnC0QDAKd5862qTWueG1lYwKTt0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=B3W4hyDKoQH49Py0YFGiwy7TxgCRhLa+e7EvAbB1QcTq+hcu7m681dRGbqzzz9/ZL6rOxWzaugqAJcIREqyCD0l5oHK4psT5IwsxVxUQu86xt1DrqgYx5aDlLWX6yjZVtvPVpPOZ8TNa4NNo0a/g9xsl0edT0eSt7Pv0HtMiVV8=
34, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
34, 25 -- s=s1024; d=yahoo.com;
34, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
34, 25 -- b=tT9XMf48xxvrleL/b6Q1+iAlljVj4Ch6GKuVjMyzirwicL0ftRL6wp2BGRaeOpiuIBcRKNROKwjrVz1cTYDqmaYPPTfCIHJWWWkHZuc9Hf9iAMTtjLtR5gLCSUC4v4hVOQWNB0KlKKarLyP/XOgK/rD/cAlOQlvd/aaR3IPoC+U=;
34, 25 -- Message-ID: {16483.57782.qm-at-web110811.mail.gq1.yahoo.com}
34, 25 -- X-YMail-OSG: FxNxFXUVM1k5GqCf5d3YPg_qQbh.YFQ.LV8S.J5WGxfxYj4TILaNlPBn
34, 25 -- Received: from [80.122.101.100] by web110811.mail.gq1.yahoo.com via HTTP; Thu, 26 Mar 2009 02:55:39 PDT
34, 25 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1
34, 25 -- References: {200903241716.n2OHGkX1012372-at-ns.microscopy.com}
34, 25 -- Date: Thu, 26 Mar 2009 02:55:39 -0700 (PDT)
34, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
34, 25 -- Subject: Re: [Microscopy] viaWWW: Fibrin clots- SEM
34, 25 -- To: dhorne-at-interchange.ubc.ca
34, 25 -- Cc: microscopy-at-microscopy.com
34, 25 -- In-Reply-To: {200903241716.n2OHGkX1012372-at-ns.microscopy.com}
34, 25 -- MIME-Version: 1.0
34, 25 -- Content-Type: text/plain; charset=iso-8859-1
34, 25 -- Content-Transfer-Encoding: 8bit
34, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2Q9tfm5009347
==============================End of - Headers==============================




From: herro001-at-umn.edu
Date: Thu, 26 Mar 2009 09:32:38 -0500
Subject: [Microscopy] Exfo X-cite 120 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

About a year ago I installed a new bulb that registered as having 400
hours on it fresh out of the box.

Last night I was about to start a timelapse and the Exfo light went
out and the display on the unit indicated over one thousand hours (it
actually had much less than that) and gave a light error.

This morning I replaced the bulb with a fresh one from a sealed box
and the Exfo unit started the bulb fine but the display indicates the
new bulb has 641 hours on it.

Taken together these three events suggest to me that the problem is
with the unit itself and not the bulbs. Has anybody else seen a
problem like this with the X-Cite 120?

Mike


Michael J. Herron, U of MN, Dept. of Entomology
herro001-at-umn.edu
612-624-3688 (office) 612-625-5299 (FAX)




==============================Original Headers==============================
10, 18 -- From herro001-at-umn.edu Thu Mar 26 09:32:37 2009
10, 18 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232])
10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QEWaXQ000327
10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 09:32:37 -0500
10, 18 -- Received: from [134.84.48.158] (x84-48-158.cfans.umn.edu [134.84.48.158])
10, 18 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP
10, 18 -- Thu, 26 Mar 2009 09:32:34 -0500 (CDT)
10, 18 -- X-Umn-Remote-Mta: [N] x84-48-158.cfans.umn.edu [134.84.48.158] #+LO+TS+AU+HN
10, 18 -- X-Umn-Classification: local
10, 18 -- Mime-Version: 1.0 (Apple Message framework v753.1)
10, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
10, 18 -- Message-Id: {003C070D-010E-4F9B-9985-24E10221E8FF-at-umn.edu}
10, 18 -- Content-Transfer-Encoding: 7bit
10, 18 -- From: Michael Herron {herro001-at-umn.edu}
10, 18 -- Subject: Exfo X-cite 120 problem
10, 18 -- Date: Thu, 26 Mar 2009 09:32:27 -0500
10, 18 -- To: Microscopy-at-microscopy.com
10, 18 -- X-Mailer: Apple Mail (2.753.1)
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 26 Mar 2009 10:25:52 -0500
Subject: [Microscopy] RE: Translation based stereo pair SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Roy,

I made for you couple stereo pairs of TEM copper grid at x50 and displacement (translation) of 500 um. First pair taken at tilt angle 0 (not much of topography, but you can measure parameters of grid from them). Second pair taken at tilt 30, so you have a lot of height difference between top and bottom of images. Displacement and tilt axis were along horizontal axis of images.

Could you explain me practical purpose of your software? Is it intended as low magnification supplement for in-lens SEM? From my estimate, parallax we have to measure is roughly equal to (t*h)/w, where h - height, t - translation, w - working distance. Suppose we acquire pictures 1000*1000 pixels wit w=10 mm. At magnification x100 field of view is about 1000 um and we have 1 um/pixel. At highest practical values of measured height h and translation t equal to half of field of view value, i.e. 500 um, parallax will be 25 um (25 pixels). Not too bad. But if we want to measure feature which height is 10 times lower (5% of field of view), then we have just 2-3 pixels of parallax, so an error of measurements will be about 50%. At magnification x1000 we should expect 50% error even for height of ½ of field of view - too high for any practical purposes. Of coarse, acquiring pictures 4000*4000 pixels will decrease error four times, and lower w, as in in-lens SEM, will improve things also.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
} Sent: Wednesday, March 25, 2009 12:59 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Translation based stereo pair SEM images
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Group,
}
} Do any of you have a few translation based (vs eucentric
} tilting) stereo pair SEM images you could share?
}
} I am evaluating some software to extract geometric
} information from these types of images.
}
} If you can help it would be greatly appreciated.
}
} Roy Beavers
} Southern Methodist University
} Department of Earth Sciences
} P.O. Box 750395
} Dallas, TX  75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-smu.edu
}
}
}
} ==============================Original
} Headers==============================
} 7, 26 -- From rbeavers-at-mail.smu.edu Wed Mar 25 12:58:50 2009
} 7, 26 -- Received: from sxet1p2.systems.smu.edu
} (sxet1p2.systems.smu.edu [129.119.65.147])
} 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2PHwnO0019930
} 7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar
} 2009 12:58:49 -0500
} 7, 26 -- Received: from sxht1p2.systems.smu.edu
} (129.119.65.133) by 7, 26 -- sxet1p2.systems.smu.edu
} (129.119.65.147) with Microsoft SMTP Server (TLS) id 7, 26 --
} 8.1.340.0; Wed, 25 Mar 2009 12:58:48 -0500 7, 26 --
} Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
} 7, 26 -- sxht1p2.systems.smu.edu ([129.119.65.133]) with
} mapi; Wed, 25 Mar 2009 7, 26 -- 12:58:40 -0500 7, 26 --
} From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 26 -- To:
} Microscopy Listserver {Microscopy-at-microscopy.com} 7, 26 --
} Date: Wed, 25 Mar 2009 12:58:40 -0500 7, 26 -- Subject:
} Translation based stereo pair SEM images 7, 26 --
} Thread-Topic: Translation based stereo pair SEM images 7, 26
} -- Thread-Index: Acmtc1RmxJZ2cgzYTBOklF23fmzjwg== 7, 26 --
} Message-ID:
} {7A6FE75608A3624E872147993C8B36BB41C22DB037-at-SXMBXC.systems.smu.edu}
} 7, 26 -- Accept-Language: en-US
} 7, 26 -- Content-Language: en-US
} 7, 26 -- X-MS-Has-Attach:
} 7, 26 -- X-MS-TNEF-Correlator:
} 7, 26 -- acceptlanguage: en-US
} 7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
} 7, 26 -- MIME-Version: 1.0
} 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 --
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n2PHwnO0019930
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
9, 25 -- From DusevichV-at-umkc.edu Thu Mar 26 10:25:51 2009
9, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QFPn9j015962
9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 10:25:50 -0500
9, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 25 -- Thu, 26 Mar 2009 10:25:48 -0500
9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 25 -- Content-class: urn:content-classes:message
9, 25 -- MIME-Version: 1.0
9, 25 -- Content-Type: text/plain;
9, 25 -- charset="iso-8859-1"
9, 25 -- Subject: RE: [Microscopy] Translation based stereo pair SEM images
9, 25 -- Date: Thu, 26 Mar 2009 10:25:48 -0500
9, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB806-at-KC-MSX1.kc.umkc.edu}
9, 25 -- In-Reply-To: {200903251759.n2PHxNUF020787-at-ns.microscopy.com}
9, 25 -- X-MS-Has-Attach:
9, 25 -- X-MS-TNEF-Correlator:
9, 25 -- Thread-Topic: [Microscopy] Translation based stereo pair SEM images
9, 25 -- Thread-Index: Acmtc3EwX2mo0aU5RCK6v8OWzcayDgAs6POA
9, 25 -- References: {200903251759.n2PHxNUF020787-at-ns.microscopy.com}
9, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
9, 25 -- To: {Microscopy-at-microscopy.com}
9, 25 -- X-OriginalArrivalTime: 26 Mar 2009 15:25:48.0797 (UTC) FILETIME=[240402D0:01C9AE27]
9, 25 -- Content-Transfer-Encoding: 8bit
9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2QFPn9j015962
==============================End of - Headers==============================




From: A.MARDINLY-at-numonyx.com
Date: Thu, 26 Mar 2009 12:13:07 -0500
Subject: [Microscopy] viaWWW: EDS window

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ahmad;
Your detector window is most likely MOXTEK, an aluminum
oxide/polymer multilayer composite supported by a silicon grid. You can
read about it at:
www.moxtek.com/PDF/Windows/AP3%20Window.pdf


John Mardinly,
Numonyx


-----Original Message-----
X-from: ahmad_ds-at-yahoo.com [mailto:ahmad_ds-at-yahoo.com]
Sent: Wednesday, March 25, 2009 5:25 AM
To: MARDINLY, A

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkhaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] EDS window

Question: I work on and EDS manufactured by EDAX of the model XL-30
SUTW. I want to know what is the material of fabrication of the super
ultra-thin window of the EDS I have.

Thank you.

Login Host: 87.236.233.99
------------------------------------------------------------------------
---

==============================Original
Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 07:16:50 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2PCGmWA019489
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009
07:16:49 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p0624080bc5efd11fda7e-at-[206.69.208.22]}
7, 11 -- Date: Wed, 25 Mar 2009 07:16:47 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: EDS window
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================



==============================Original Headers==============================
17, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 26 12:13:06 2009
17, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QHD6Kq000922
17, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 26 Mar 2009 12:13:06 -0500
17, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24])
17, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id BAF0614400B;
17, 29 -- Thu, 26 Mar 2009 11:13:09 -0400 (EDT)
17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959);
17, 29 -- Thu, 26 Mar 2009 13:13:05 -0400
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: RE: [Microscopy] viaWWW: EDS window
17, 29 -- Date: Thu, 26 Mar 2009 13:12:11 -0400
17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9FD2BCF-at-EXDRESBENMX012.numonyx.local}
17, 29 -- In-Reply-To: {200903251224.n2PCOqYI031112-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: [Microscopy] viaWWW: EDS window
17, 29 -- Thread-Index: AcmtRLUEHPA2jov+Td+MrzCHQprA8gA8PyNw
17, 29 -- References: {200903251224.n2PCOqYI031112-at-ns.microscopy.com}
17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com}
17, 29 -- To: {ahmad_ds-at-yahoo.com}
17, 29 -- Cc: {Microscopy-at-Microscopy.com}
17, 29 -- X-OriginalArrivalTime: 26 Mar 2009 17:13:05.0456 (UTC) FILETIME=[20903B00:01C9AE36]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2QHD6Kq000922
==============================End of - Headers==============================




From: murphyjudy-at-comcast.net
Date: Thu, 26 Mar 2009 16:13:14 -0500
Subject: [Microscopy] Lee Dreyer Walks With Angels, Service Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lee worked in the microscopy field from 1968 to 2004, developing
instrumentation, and training hundreds of students.


Leroy Louis Dreyer (Lee)
Sept. 18, 1925 - Mar 18, 2009

Lee Dreyer, 83, passed away peacefully, in his wife's arms in Stockton,
CA, on Mar 18, 2009 after a courageous 14 month battle with cancer. He
was born on Sept 18, 1925 in St. Louis, MO, a birthday he shared with
his late mother, Olga Goedecke, of St. Louis. He was also preceded in
death by: his father & stepmother, Louis & Marie Dreyer, of St. Louis;
and his son, David Dreyer of Champaign, IL. He is survived by his
beloved family and friends which include: his wife, Judy Murphy
(Stockton, CA); sister, Dolores Kornfeld (Buffalo, NY); 3 daughters -
Sandy & Nat Simpkins(Manchester, MA) - Karen & Bob Graham and Joni &
Brad Hendricks, all of Champaign, IL; 5 grandchildren - Chris Nelson
(New Hampshire), David Hartley (Bolivia, SA), and Davena, Bobby &
Michael Graham (Champaign, IL); 5 great-grandchildren - Adam & Zoe
Nelson (New Hampshire), Andrea & David John Hartley (Bolivia, SA), and
Sydnee Graham (North Carolina); and 2 nieces - Suzanne and Marilyn
Kornfeld and 1 nephew - Robert Kornfeld.

EDUCATION: Lee graduated from Hadley Vocational Technical School
(St. Louis) specializing in Electrical & Radio Repair in 1942. He
joined the Army Signal Corps (Rolla, MO) in Communication and Pre-radar
followed by enlisting in the Navy in 1943. He attended US Navy Service
Schools in Airborne Communication & Radar and served in the
Asian/Pacific in World War 2. He was an Aviation Electronic Technician
Mate, First Class, and did aircraft maintenance on the Marianas Islands
of Tinian, Saipan, and Guam. After the war, Lee received his Assoc. of
Arts in Pre-engineering at Harris Teachers Col, St. Louis, and his BS in
Electrical Engineering at the University of Ill, Urbana, IL in 1956. In
1984, Lee received certification in Digital & Microprocessor Electronics
at Missouri Tech. Sch., St. Louis.

WORK EXPERIENCE: From 1947-52, Lee worked as an Engineer at
Western Electric, Emerson Electric & Vickers Electric, all in St.
Louis. University of Illinois (Urbana,IL): 1952-68, Bio-Physics Lab,
and the Gaseous Electronics Lab as Electronics Engineer; 1968-71, Center
for Electron Microscopy as EM Engineer/Instructor. Southern Illinois
University: 1971-83 (Carbondale), Center for Electron Microscopy as EM
Engineer/Instructor; 1986-1992 (Edwardsville), School of Science as
Electronics Engineer. 1994-2004, San Joaquin Delta College (Stockton,
CA), Microscopy Technology Center in Microscopy Instruction.

HONORS: Lee is co-inventor and has several patents (1952-62) as
well as articles in scientific journals with Drs. William and Frank Fry,
& Dr. Russell Meyers, on ultrasonic irradiation in hyperkinetic and
hypertonic disorders (Parkinson's Disease).

Lee truly enjoyed vocational education. He was always involved in
training students to use instrumentation but came into his own when
training researchers/students in electron microscopy. He thoroughly
enjoyed watching his students blossom as they mastered the instruments.
He had the patience of Job during his one on one sessions. His students
loved him and appreciated his patience, expert help and compassion as
well as his unique sense of humor.

Lee also enjoyed developing instrumentation for various projects.
He always said that he fixed everything Judy broke!!!!!

HOBBIES: Lee enjoyed fishing, boating, scuba diving, and
traveling. Lee and his wife Judy, traveled throughout their 39 yrs
together, circumnavigating the globe, and living life to its fullest.

His heart, his kindness, his honesty, his incredible sense of humor,
and his compassion touched all who knew him. Lee will be in our hearts
and minds forever. He was loved by all and will be dearly missed. He
truly does walk with angels now.

SERVICES:
Funeral Home: Kutis Funeral Home, 10151 Gravois Rd, Affton, MO 63123
(314/842-4458).
Visitation: April 2, 2009: 3-9 pm
Chapel Service (at Kutis): April 3, 2009: 11:30 am
Interment: April 3, 2009 (after chapel service) Jefferson Barracks
National Cemetery, 2900 Sheridan Rd., St. Louis, MO 63125
In lieu of flowers, donations can be made to the Lee Dreyer Memorial
Fund. A memorial garden will be made at his home. Send donations to
Lee Dreyer Memorial Fund, PMB# 119, 4719 Quail Lakes Dr., Ste G,
Stockton, CA 95207-5267.


==============================Original Headers==============================
13, 20 -- From murphyjudy-at-comcast.net Thu Mar 26 16:13:13 2009
13, 20 -- Received: from QMTA12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QLDDoq024647
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 16:13:13 -0500
13, 20 -- Received: from OMTA01.emeryville.ca.mail.comcast.net ([76.96.30.11])
13, 20 -- by QMTA12.emeryville.ca.mail.comcast.net with comcast
13, 20 -- id XsPK1b01t0EPchoACxDEj5; Thu, 26 Mar 2009 21:13:14 +0000
13, 20 -- Received: from [192.168.1.3] ([76.127.99.233])
13, 20 -- by OMTA01.emeryville.ca.mail.comcast.net with comcast
13, 20 -- id XxDD1b00K527pzg8MxDDnt; Thu, 26 Mar 2009 21:13:14 +0000
13, 20 -- Message-ID: {49CBEFEA.4050004-at-comcast.net}
13, 20 -- Date: Thu, 26 Mar 2009 14:13:14 -0700
13, 20 -- From: Judy Murphy {murphyjudy-at-comcast.net}
13, 20 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2
13, 20 -- X-Accept-Language: en-us, en
13, 20 -- MIME-Version: 1.0
13, 20 -- To: Microscopy {Microscopy-at-microscopy.com}
13, 20 -- Subject: Lee Dreyer Walks With Angels, Service Info
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
13, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Klugem07-at-bu.edu
Date: Thu, 26 Mar 2009 19:18:07 -0500
Subject: [Microscopy] viaWWW: EM Image Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Klugem07-at-bu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Klugem07-at-bu.edu
Name: Matt

Organization: Boston University Medical Center

Title-Subject: [Filtered] EM Image Calibration

Question: Recently, I have been tracing EM images of organelles in
white cells, using ImageJ.

I need help calibrating my images, because it is becoming clear that
merely using the scale provided in the image is not accutate.

Currently, I am analyzing images at two different magnification settings.

With the basic calibration method I have been using, It seems that
the organelle areas are approximately half as large at a direct
magnification of 30000x and 59900 x 8.0in print magnification as they
are at 12000x magnification and 177000 x 8.0in print magnification.

I have spoken to someone at our Medical Center who suggested a simple
print mag formula, Print mag = length of structure on print / actual
length.

As I have already mentioned, one of the values I am obtaining is
area, but I'm afraid that this formula will not help me for a 2-D
measurement.

Any suggestions? Thanks.



Login Host: 155.41.112.1
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Thu Mar 26 19:18:07 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2R0I6NV010946
14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 19:18:06 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240800c5f1cbac95de-at-[206.69.208.22]}
14, 11 -- Date: Thu, 26 Mar 2009 19:18:04 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: Klugem07-at-bu.edu (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: EM Image Calibration
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 27 Mar 2009 05:41:34 -0500
Subject: [Microscopy] Re: viaWWW: EM Image Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Matt,
When I started measuring carbon black by TEM, to assure myself of the
calibration, I printed images of my calibration grid at the magnifications
I was interested in and chech them witha ruler and calculator. I also
reran those images with my on-line measuring program to confirm the
electronic measurements. As a last step I ran spheres from Duke Scientific
to assure myself that both the system and I was performing the measurements
correctly.

stay safe.........
Frank



Klugem07-at-bu.edu

03/26/2009 08:25 To
PM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
Klugem07-at-bu.edu [Microscopy] viaWWW: EM Image
Calibration













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both Klugem07-at-bu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Klugem07-at-bu.edu
Name: Matt

Organization: Boston University Medical Center

Title-Subject: [Filtered] EM Image Calibration

Question: Recently, I have been tracing EM images of organelles in
white cells, using ImageJ.

I need help calibrating my images, because it is becoming clear that
merely using the scale provided in the image is not accutate.

Currently, I am analyzing images at two different magnification settings.

With the basic calibration method I have been using, It seems that
the organelle areas are approximately half as large at a direct
magnification of 30000x and 59900 x 8.0in print magnification as they
are at 12000x magnification and 177000 x 8.0in print magnification.

I have spoken to someone at our Medical Center who suggested a simple
print mag formula, Print mag = length of structure on print / actual
length.

As I have already mentioned, one of the values I am obtaining is
area, but I'm afraid that this formula will not help me for a 2-D
measurement.

Any suggestions? Thanks.



Login Host: 155.41.112.1
---------------------------------------------------------------------------

==============================Original
Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Thu Mar 26 19:18:07 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n2R0I6NV010946
14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009
19:18:06 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240800c5f1cbac95de-at-[206.69.208.22]}
14, 11 -- Date: Thu, 26 Mar 2009 19:18:04 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: Klugem07-at-bu.edu (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: EM Image Calibration
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
31, 22 -- From frank_karl-at-lincolnelectric.com Fri Mar 27 05:41:34 2009
31, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
31, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RAfYXS013205
31, 22 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 05:41:34 -0500
31, 22 -- In-Reply-To: {200903270025.n2R0PMZF023384-at-ns.microscopy.com}
31, 22 -- Subject: Re: [Microscopy] viaWWW: EM Image Calibration
31, 22 -- To: Klugem07-at-bu.edu, Microscopy-at-microscopy.com
31, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
31, 22 -- Message-ID: {OF12DECAFE.78888A27-ON85257586.003A36FB-85257586.003AB5CA-at-lincolnelectric.com}
31, 22 -- Date: Fri, 27 Mar 2009 06:41:11 -0400
31, 22 -- From: Frank_Karl-at-lincolnelectric.com
31, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM,
31, 22 -- CD-MIME complete at 03/27/2009 06:41:10 AM,
31, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM,
31, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM,
31, 22 -- Serialize complete at 03/27/2009 06:41:10 AM
31, 22 -- MIME-Version: 1.0
31, 22 -- Content-Type: text/plain;
31, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Fri, 27 Mar 2009 08:53:45 -0500
Subject: [Microscopy] Philips CM12 trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your figures suggest that a 30,000x picture enlarged is 59,900x but
your 12,000x picture enlarged is 177,000x. I assume there is a zero
missing or added somewhere or I've misunderstood.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
X-from: Klugem07-at-bu.edu

Hello,
Following error appeared two times in the last two days on our Philips CM12
OPCON display: "ERROR MESSAGE: SOLID-STATE-KEY".
After that, the microscope did not response to any key (button) with
exception of RESTART and OFF. The user manual states: Contact service
department.
Does anybody has experiences with this error? Thanks for any suggestions.

With best regards Oldrich

--
Oldřich Benada
Institute of Microbiology, Acad. Sci. CR, v.v.i.
Videnska 1083
142 20 Prague 4 - Krc
Czech Republic


==============================Original Headers==============================
4, 23 -- From benada-at-biomed.cas.cz Fri Mar 27 08:53:45 2009
4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RDrj73014355
4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 08:53:45 -0500
4, 23 -- Received: from u117ob.mbu.cas.cz (u117ob.mbu.cas.cz [147.231.44.101])
4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
4, 23 -- (No client certificate requested)
4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 0910A1A445A5
4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 14:53:44 +0100 (CET)
4, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
4, 23 -- Reply-To: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz}
4, 23 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i.
4, 23 -- To: microscopy-at-microscopy.com
4, 23 -- Subject: Philips CM12 trouble
4, 23 -- Date: Fri, 27 Mar 2009 14:53:04 +0100
4, 23 -- User-Agent: KMail/1.9.9
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: text/plain;
4, 23 -- charset="utf-8"
4, 23 -- Content-Disposition: inline
4, 23 -- Message-Id: {200903271453.04622.benada-at-biomed.cas.cz}
4, 23 -- Content-Transfer-Encoding: 8bit
4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RDrj73014355
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Fri, 27 Mar 2009 09:54:25 -0500
Subject: [Microscopy] Re: viaWWW: EM Image Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Matt,

I am not sure of your procedure. However, the magnification formula is
correct.

Here's how I always calculated and converted between micrometer distances
and mags.

General Rule Procedure:
Take the magnification and convert it to KX. Let's say you get 50 KX.
That number in millimeters is the length of ONE micron at that mag of 50 KX.
So in this example, 50 mm is one micrometer (or 5 mm is 0.1 µm).
This mag rule works for TEM, SEM, OM, et al.

You could try to draw a calibration bar 50 mm long and label it as 1 µm,
but the print is not that wide. So use one-tenth of that millimeter
distance and and label it as 0.1µm.
Always determine the one micron distance first and then convert up or down
from there. This procedure can be used in the reverse order and is the
more common procedure.

Here's a few example of how to use this procedure or rule.
Let's say you see an image in Microscopy Today that shows an internal
calibration bar in the image and it says 0.1 µm. So what's the
magnification in MT? Did the author know how much his image would be
reduced or enlarged? No.
Say the 0.1 µm bar is 6 millimeters long.
Convert that to one micron and you get 60 millimeters for one micrometer.
The mag of the image in MT is 60 KX regardless of what the labeling under
the image says or the article.

Here's an optical micrograph calculation example. Your print was
determined by a stage micrometer calibration to be 37 X. That's 0.037 KX.
So one micron is 0.037 mm but you can't draw that length.
So use 3.7 mm and label the bar as 100 microns.

To calibrate an optical microscope, you need a calibration standard called
a stage micrometer.
Mag*I*Cal is a calibration standard for TEM but most microscopists still
use the common carbon grating replica (cheaper).
Beautifully made NIST traceable step standards can be purchased from VLSI
(vlsistandards.com) for all types of measurements. We used one for Mirau
interferometry. The LVSI standards are not cheap but they are certified
traceable and VLSI is ISO9001 certified.

For image analysis, you need to photograph a standard and "pull" it into
your CRT with your software. Then you can calibrate your software directly
to your known distance standard.

This mag rule is very helpful and easy to use "on the fly". I gave a
presentation with a 35 mm slide of a TEM micrograph displayed it onto a
large projector screen. Some guy asked, "What is the actual mag on the
screen?" I picked up a meter stick, measured the distance for the 0.1 µm
calibration bar as 150 mm, multiplied by ten to get the one micron
distance, multiplied by 1000, and I said, "Close to 1.5 million X."

Paul Beauregard

At 07:18 PM 3/26/09 -0500, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
12, 29 -- From beaurega-at-westol.com Fri Mar 27 09:54:25 2009
12, 29 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4])
12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2REsORt030548
12, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 09:54:24 -0500
12, 29 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
12, 29 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n2REsB3e032178
12, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 10:54:13 -0400
12, 29 -- Received: (qmail 8710 invoked by uid 89); 27 Mar 2009 14:54:07 -0000
12, 29 -- Received: from pitts-69-72-13-253.dynamic-dialup.coretel.net (HELO running) (69.72.13.253)
12, 29 -- by mail.winbeam.com with SMTP; 27 Mar 2009 14:54:07 -0000
12, 29 -- Message-Id: {3.0.6.32.20090327115417.00857740-at-pop3.norton.antivirus}
12, 29 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus
12, 29 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
12, 29 -- Date: Fri, 27 Mar 2009 11:54:17 -0500
12, 29 -- To: Klugem07-at-bu.edu, microscopy-at-microscopy.com
12, 29 -- From: Beaurega {beaurega-at-westol.com}
12, 29 -- Subject: Re: [Microscopy] viaWWW: EM Image Calibration
12, 29 -- In-Reply-To: {200903270018.n2R0IarF011566-at-ns.microscopy.com}
12, 29 -- Mime-Version: 1.0
12, 29 -- Content-Type: text/plain; charset="iso-8859-1"
12, 29 -- Content-Transfer-Encoding: 8bit
12, 29 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
12, 29 -- X-Winbeam-MailScanner-ID: n2REsB3e032178
12, 29 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
12, 29 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted),
12, 29 -- SpamAssassin (not cached, score=-0.882, required 4, AWL -0.69,
12, 29 -- BAYES_00 -2.00, SARE_SPEC_REPLICA_OBFU 1.81)
12, 29 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
12, 29 -- X-Winbeam-MailScanner-Watermark: 1238770453.86976-at-k8OsoFJYgNAWQ3IGJNOluA
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Fri, 27 Mar 2009 11:06:28 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).

I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.

But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"

Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for).
Link to image (I have a few more images):
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg

I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.

Thanks!

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28 2009
9, 28 -- Received: from honeydew.services.brown.edu (honeydew.services.brown.edu [128.148.106.194])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RG6RN8014388
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 11:06:28 -0500
9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53])
9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3) with ESMTP id n2RG6PC6031327
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 28 -- Content-class: urn:content-classes:message
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- Subject: SEM artifact help wanted
9, 28 -- Date: Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu}
9, 28 -- X-MS-Has-Attach:
9, 28 -- X-MS-TNEF-Correlator:
9, 28 -- Thread-Topic: SEM artifact help wanted
9, 28 -- Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA==
9, 28 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
9, 28 -- To: {microscopy-at-microscopy.com}
9, 28 -- X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC) FILETIME=[FB87A080:01C9AEF5]
9, 28 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 definitions=5565 signatures=515473
9, 28 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903270105
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RG6RN8014388
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Fri, 27 Mar 2009 12:34:56 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just wanted to follow up and say I was there overseeing the CPD of these cells and I'm very confident in this particular process. It is a Ladd unit (Polaron) and I've been using these with great success since '92 or so. Maybe '93? (Long ago enough that I don't think one or two years makes much of a difference).

I observed the CPD run this time and it couldn't have been better from a temp/pressure/flush/vent process.

(Yes, I should have added this the first time - sorry).

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University

http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/

-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Friday, March 27, 2009 12:12 PM
To: Williams, Geoffrey

We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).

I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.

But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"

Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for).
Link to image (I have a few more images):
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg

I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.

Thanks!

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28 2009
9, 28 -- Received: from honeydew.services.brown.edu (honeydew.services.brown.edu [128.148.106.194])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RG6RN8014388
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 11:06:28 -0500
9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53])
9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3) with ESMTP id n2RG6PC6031327
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 28 -- Content-class: urn:content-classes:message
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- Subject: SEM artifact help wanted
9, 28 -- Date: Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu}
9, 28 -- X-MS-Has-Attach:
9, 28 -- X-MS-TNEF-Correlator:
9, 28 -- Thread-Topic: SEM artifact help wanted
9, 28 -- Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA==
9, 28 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
9, 28 -- To: {microscopy-at-microscopy.com}
9, 28 -- X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC) FILETIME=[FB87A080:01C9AEF5]
9, 28 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 definitions=5565 signatures=515473
9, 28 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903270105
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RG6RN8014388
==============================End of - Headers==============================


==============================Original Headers==============================
19, 30 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 12:34:53 2009
19, 30 -- Received: from beaker.services.brown.edu (beaker.services.brown.edu [128.148.106.193])
19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RHYpWo003375
19, 30 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:34:52 -0500
19, 30 -- Received: from ex-gateway1-out.AD.Brown.Edu (ex-gateway1.ad.brown.edu [128.148.21.51])
19, 30 -- by beaker.services.brown.edu (8.14.3/8.14.3) with ESMTP id n2RHYlIJ006378
19, 30 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 13:34:47 -0400
19, 30 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by ex-gateway1-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
19, 30 -- Fri, 27 Mar 2009 13:34:47 -0400
19, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 30 -- Content-class: urn:content-classes:message
19, 30 -- MIME-Version: 1.0
19, 30 -- Content-Type: text/plain;
19, 30 -- charset="iso-8859-1"
19, 30 -- Subject: [Microscopy] SEM artifact - addendum
19, 30 -- Date: Fri, 27 Mar 2009 13:34:47 -0400
19, 30 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0792D997-at-MAIL1.AD.Brown.Edu}
19, 30 -- In-Reply-To: {200903271611.n2RGBqsl019819-at-ns.microscopy.com}
19, 30 -- X-MS-Has-Attach:
19, 30 -- X-MS-TNEF-Correlator:
19, 30 -- Thread-Topic: [Microscopy] SEM artifact - addendum
19, 30 -- Thread-Index: Acmu9r90nJOJP+49SSypn9GM7dcKgQACp2dg
19, 30 -- References: {200903271611.n2RGBqsl019819-at-ns.microscopy.com}
19, 30 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
19, 30 -- To: {microscopy-at-microscopy.com}
19, 30 -- X-OriginalArrivalTime: 27 Mar 2009 17:34:47.0593 (UTC) FILETIME=[531C5190:01C9AF02]
19, 30 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 definitions=5565 signatures=515473
19, 30 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903270120
19, 30 -- Content-Transfer-Encoding: 8bit
19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RHYpWo003375
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 27 Mar 2009 12:37:50 -0500
Subject: [Microscopy] Re: SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geoff,

I've seen this lots of time from when I was doing cells.
Any or all of fixation, dehydration, and CPD
could cause this. Also post-CPD handling,
meaning, are they wearing gloves? Are they
keeping the samples in containers in desiccators
and never leaving them sit outside?
Fixation:
Are they using 1% monomeric tannic acid in the
glut and maybe also in the osmium?
The glut should be 1%, maybe 1.25%, no more. Fix for one hour, 2 at most.
Dehydration:
Spread cells on substrate dehydrate quickly, so 3
or 5 minutes per step is enough,
50-70-80-90-95-3X100% EtOH. Might have to start
at 30% EtOH or even lower. Some folks will do 5%
steps.
CPD:
Most CPD manufacturers use the wrong steps for
drying. The lqCO2 has to be infiltrated and
exchanged for the EtOH in the same way the EtOH
has to be exchanged for water in the cells. Use X
changes for Y minutes each, depending on samples.
For cells on impermeable substrates (metal,
glass), I used 3 changes ("soaks") for 5 minutes
each. Depending on how many samples and how
packed they were. Another possible source of the
problem -- the lqCO2 and EtOH have to flow freely
around the samples to properly exchange,
otherwise EtOH is left behind. Make sure to purge
well between lqCO2 changes -- 2-3 minutes,
usually.
/And/ be sure to lower the pressure SLOWLY once
the critical point is exceeded. "Too slowly"
can't happen.
Mind, the cracks being mostlly in the thin,
spread regions do make me think they could be due
to incomplete dehydration, or extraction during
dehydration.
But, the problem could come from any of the 3 main steps above.
What is the method they used?

Phil

} We have someone here who is trying to image
} cells grown on Titanium squares, both flat and
} micro-structured surfaces. They were getting
} decent results at one point and lately, or
} really the last month or two they have been
} chasing problems (as I understand the situation).
}
} I've looked over the protocol and it seems, to
} me, to be correct. The samples are chemically
} fixed (buffered Glute and OsO4), dehydrated
} (ETOH), CPD, coated and imaged.
}
} But without going into more details I'm just
} wondering if someone out here might be able to
} look at the image and say "Oh yeah, I know
} exactly what the problem is and how to fix it!"
}
} Hopefully though that solution doesn't involve
} high vacuum freeze drying or cryo-sem (neither
} of which we have the equipment for).
} Link to image (I have a few more images):
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg
}
} I am not personally doing the prep, but have
} been asked to see what can be done to help, esp
} since they are most interested in keeping the
} cellular projections intact. It may be a very
} simple issue but honestly maybe I've had too
} much botanical training in terms of sample prep
} and am missing it.
}
} Thanks!
}
} Geoff Williams
} Leduc Bioimaging Facility Manager
} Brown University
} Ý
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
5, 28 -- From oshel1pe-at-cmich.edu Fri Mar 27 12:37:50 2009
5, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RHbnWm007188
5, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:37:50 -0500
5, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n2RHbht3018150;
5, 28 -- Fri, 27 Mar 2009 13:37:43 -0400
5, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 28 -- Fri, 27 Mar 2009 13:37:28 -0400
5, 28 -- Mime-Version: 1.0
5, 28 -- Message-Id: {f0624080bc5f2b941cfd9-at-[141.209.160.249]}
5, 28 -- In-Reply-To: {200903271612.n2RGCaAI020365-at-ns.microscopy.com}
5, 28 -- References: {200903271612.n2RGCaAI020365-at-ns.microscopy.com}
5, 28 -- Date: Fri, 27 Mar 2009 13:37:25 -0400
5, 28 -- To: Geoffrey_Williams-at-brown.edu
5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 28 -- Subject: Re: [Microscopy] SEM artifact help wanted
5, 28 -- Cc: Microscopy-at-microscopy.com
5, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
5, 28 -- X-OriginalArrivalTime: 27 Mar 2009 17:37:28.0715 (UTC) FILETIME=[B32595B0:01C9AF02]
5, 28 -- X-Canit-CHI2: 0.00
5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 28 -- X-Spam-Score: -2.60 () [Hold at 5.00] L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
5, 28 -- X-CanItPRO-Stream: default
5, 28 -- X-Canit-Stats-ID: 10887683 - d0d6b0e5f639
5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
5, 28 -- Content-Transfer-Encoding: 8bit
5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RHbnWm007188
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Fri, 27 Mar 2009 12:46:52 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with Phil - there are many factors, But the one that made the most
difference for me recently was to do the dehydration in much smaller
steps, starting with 10%. And I know Phil has shown that soaks in the
lqCO2 really help!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



==============================Original Headers==============================
5, 20 -- From tina-at-pbrc.hawaii.edu Fri Mar 27 12:46:52 2009
5, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RHkp54029194
5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:46:52 -0500
5, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
5, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n2RHklDj016265
5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 07:46:48 -1000 (HST)
5, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n2RHklGQ016262
5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 07:46:47 -1000 (HST)
5, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
5, 20 -- Date: Fri, 27 Mar 2009 07:46:46 -1000 (HST)
5, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
5, 20 -- X-Sender: tina-at-halia
5, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
5, 20 -- Subject: Re: [Microscopy] Re: SEM artifact help wanted
5, 20 -- In-Reply-To: {200903271738.n2RHcihW009490-at-ns.microscopy.com}
5, 20 -- Message-ID: {Pine.GSO.4.21.0903270743080.16239-100000-at-halia}
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: rcmoretz-at-gmail.com
Date: Fri, 27 Mar 2009 13:48:43 -0500
Subject: [Microscopy] Books looking for a home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have some EM books that need to find a new address. Available first
come first serve. They will be sent out media mail.

Fundamentals of Transmission Electron Microscopy, by R D Heidenreich,
publ 1964. Has dust jacket


Some Biological Techniques in Electron Microscopy, ed by D F Parsons, publ 1970


Optics of the Electromagnetic Spectrum, by C L Andrews, publ 1960


EMSA and Its People; The First Fifty Years, by S P Newberry, publ 1992


Downsizing means making some hard choices.

Roger Moretz

PS Include your mailing address with your request if you will please.

==============================Original Headers==============================
12, 31 -- From rcmoretz-at-gmail.com Fri Mar 27 13:48:43 2009
12, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.144])
12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RImgIf014982
12, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 13:48:43 -0500
12, 31 -- Received: by qw-out-1920.google.com with SMTP id 5so892483qwc.54
12, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 11:48:42 -0700 (PDT)
12, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 31 -- d=gmail.com; s=gamma;
12, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
12, 31 -- :from:to:content-type:content-transfer-encoding;
12, 31 -- bh=c+CGbf7NjCClehnXhcqkCNxngZta4BAVoj2cNIra1VA=;
12, 31 -- b=E4zZkfXNibdLdD4EfebXcRMnW2QgKJYSscmjO3FAD0zhcynXJ75u0Vrpflpv/iub5q
12, 31 -- M/o28QFLIMs0FSh7lzmgFY/1bjstl3c2HJHnwR4AtmSjoHfOOA0K7OZsbXMigxbrdzZv
12, 31 -- 9wEiTO4biB0z4vCuRBZikMAnOPGM0rnIGoYSg=
12, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
12, 31 -- d=gmail.com; s=gamma;
12, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
12, 31 -- :content-transfer-encoding;
12, 31 -- b=LoI1lVz2d/JJ72bSn/7KZsCxK5xm5k90fnP+gpZPMSZIYIWwjjKYYxHPvbl8yQnTWs
12, 31 -- HF/1gTDgbc7rzr1iy9gdylVhprXYATvQ2+cTnx+AZIxiL8TEvNX0C4eXdV/G45flHbrV
12, 31 -- dNgCnyLBMxu1X5j85iRS+cwrRS78Ly1RYko1Q=
12, 31 -- MIME-Version: 1.0
12, 31 -- Received: by 10.229.74.79 with SMTP id t15mr1207808qcj.2.1238179718899; Fri,
12, 31 -- 27 Mar 2009 11:48:38 -0700 (PDT)
12, 31 -- Date: Fri, 27 Mar 2009 14:48:38 -0400
12, 31 -- Message-ID: {950e3cfd0903271148h4e8c10ajf43e0210ea9a98eb-at-mail.gmail.com}
12, 31 -- Subject: Books looking for a home
12, 31 -- From: Roger Moretz {rcmoretz-at-gmail.com}
12, 31 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
12, 31 -- Content-Type: text/plain; charset=ISO-8859-1
12, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Fri, 27 Mar 2009 14:10:57 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geoff
I have often seen this artifact on cultured cells processed for SEM.
I believe the problem may be due to allowing the cells to 'dry out' between
changes of the higher percentages (95 and 100) of ETOH or when the cells are
transferred to the CPD chamber. It only take a couple of seconds for the
thin layer of ETOH to evaporate off the cells leading to this nicely
crackled surface.

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca


-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Friday, March 27, 2009 12:17 PM
To: rjharris-at-uwo.ca

We have someone here who is trying to image cells grown on Titanium squares,
both flat and micro-structured surfaces. They were getting decent results
at one point and lately, or really the last month or two they have been
chasing problems (as I understand the situation).

I've looked over the protocol and it seems, to me, to be correct. The
samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH),
CPD, coated and imaged.

But without going into more details I'm just wondering if someone out here
might be able to look at the image and say "Oh yeah, I know exactly what the
problem is and how to fix it!"

Hopefully though that solution doesn't involve high vacuum freeze drying or
cryo-sem (neither of which we have the equipment for).
Link to image (I have a few more images):
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.j
pg

I am not personally doing the prep, but have been asked to see what can be
done to help, esp since they are most interested in keeping the cellular
projections intact. It may be a very simple issue but honestly maybe I've
had too much botanical training in terms of sample prep and am missing it.

Thanks!

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28 2009
9, 28 -- Received: from honeydew.services.brown.edu
(honeydew.services.brown.edu [128.148.106.194])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2RG6RN8014388
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 11:06:28
-0500
9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu
(ex-gateway2.ad.brown.edu [128.148.21.53])
9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3) with ESMTP id
n2RG6PC6031327
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:06:26
-0400
9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by
ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 28 -- Content-class: urn:content-classes:message
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- Subject: SEM artifact help wanted
9, 28 -- Date: Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Message-ID:
{A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu}
9, 28 -- X-MS-Has-Attach:
9, 28 -- X-MS-TNEF-Correlator:
9, 28 -- Thread-Topic: SEM artifact help wanted
9, 28 -- Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA==
9, 28 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
9, 28 -- To: {microscopy-at-microscopy.com}
9, 28 -- X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC)
FILETIME=[FB87A080:01C9AEF5]
9, 28 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00
definitions=5565 signatures=515473
9, 28 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam
policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0
adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000
definitions=main-0903270105
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2RG6RN8014388
==============================End of - Headers==============================



==============================Original Headers==============================
20, 29 -- From rjharris-at-uwo.ca Fri Mar 27 14:10:57 2009
20, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RJAuCq029444
20, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 14:10:57 -0500
20, 29 -- MIME-version: 1.0
20, 29 -- Content-type: text/plain; charset=iso-8859-1
20, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39])
20, 29 -- by harpo.mail.uwo.pri
20, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
20, 29 -- with ESMTP id {0KH6002UGILPHY90-at-harpo.mail.uwo.pri} for
20, 29 -- Microscopy-at-microscopy.com; Fri, 27 Mar 2009 15:10:37 -0400 (EDT)
20, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
20, 29 -- by harpo.mail.uwo.pri
20, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
20, 29 -- with ESMTPSA id {0KH600LVPILPLW00-at-harpo.mail.uwo.pri} for
20, 29 -- Microscopy-at-microscopy.com; Fri, 27 Mar 2009 15:10:37 -0400 (EDT)
20, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
20, 29 -- To: Geoffrey_Williams-at-brown.edu
20, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
20, 29 -- References: {200903271617.n2RGHIZo027419-at-ns.microscopy.com}
20, 29 -- In-reply-to: {200903271617.n2RGHIZo027419-at-ns.microscopy.com}
20, 29 -- Subject: RE: [Microscopy] SEM artifact help wanted
20, 29 -- Date: Fri, 27 Mar 2009 15:10:38 -0400
20, 29 -- Message-id: {00a401c9af0f$b73e6660$25bb3320$-at-ca}
20, 29 -- X-Mailer: Microsoft Office Outlook 12.0
20, 29 -- Thread-index: Acmu94SBB/3E2nkuQ6SoRCYUyavUYgAF4+RQ
20, 29 -- Content-language: en-us
20, 29 -- Content-Transfer-Encoding: 8bit
20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RJAuCq029444
==============================End of - Headers==============================




From: rcmoretz-at-gmail.com
Date: Fri, 27 Mar 2009 14:13:27 -0500
Subject: [Microscopy] Re: Books looking for a home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for the responses, the books now have a new home.

Roger Moretz

==============================Original Headers==============================
2, 31 -- From rcmoretz-at-gmail.com Fri Mar 27 14:13:27 2009
2, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.146])
2, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RJDQDV001565
2, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 14:13:26 -0500
2, 31 -- Received: by qw-out-1920.google.com with SMTP id 5so900963qwc.54
2, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:13:25 -0700 (PDT)
2, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
2, 31 -- d=gmail.com; s=gamma;
2, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
2, 31 -- :from:to:content-type:content-transfer-encoding;
2, 31 -- bh=PRNTea8qeEN8iOWEMTLKXxZmv5z6iYeKH8AcexXw2vQ=;
2, 31 -- b=sMqoiVVwJgVcHq1O05TkX4W4qyfeGPeeRuxYQYJ1rvx62QpOeGLjrfEcVkrKq6hK/m
2, 31 -- 29MOmiZd1sNiWlltxFoep6dJRYbOybhleRcbjOY2r6yXPGxjMKEm6kJJE3J+Yh0LREtf
2, 31 -- fztGScHRjpQHqHgBzMu1/iwGANI5KepzwfY/I=
2, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
2, 31 -- d=gmail.com; s=gamma;
2, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
2, 31 -- :content-transfer-encoding;
2, 31 -- b=ixGNqT9PTFMlUJ2ioZ2V1pOuGxCwLkZmaquLuZNfYbssGaAfA2Garis0JlVywi5zQX
2, 31 -- lRDJ/SVUG6Mr+fG+qDycNE3FHxQy3EmydToJfn4D0yMVA8lqCsn2zaLN2I7SFASnylU+
2, 31 -- rofPbgoGQxPtL+cPtKPcedQxxECaetxqcdtx8=
2, 31 -- MIME-Version: 1.0
2, 31 -- Received: by 10.229.100.7 with SMTP id w7mr1213610qcn.5.1238181200600; Fri, 27
2, 31 -- Mar 2009 12:13:20 -0700 (PDT)
2, 31 -- Date: Fri, 27 Mar 2009 15:13:20 -0400
2, 31 -- Message-ID: {950e3cfd0903271213r7d1701f7s99de8f88220d7d88-at-mail.gmail.com}
2, 31 -- Subject: Re: Books looking for a home
2, 31 -- From: Roger Moretz {rcmoretz-at-gmail.com}
2, 31 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
2, 31 -- Content-Type: text/plain; charset=ISO-8859-1
2, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Fri, 27 Mar 2009 15:11:00 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Geoff
Your preparation looks fine to me so far I can zoom in your picture, and I
am trying to think: The various podia projecting from one side of each cell
are seen attached to the floor and detached from the cell's main -and nice-
body that probably overlies nucleus. At the opposite -the ugly- side the
plasma membrane is more spread like a leaf as opposed to the thread like
projections of the other side. It is the leaf side I suspect people do not
like.
But animal cells shrink a lot during dehydration and drying while we mummify
them for SEM. More than a dried leaf. The cells of the picture look strongly
attached to the substratum and shrinkage damaged them in both sides, the
podia were broken, the leaf-like membrane was fragmented.
This is how it happens when you prepare animal cells on solid substrates for
SEM, you subject them big forces like cell to substratum adherence versus
cellular integrity. I don't understand why your titanum client is not happy
with these results; running controls like plastic could help him aqcuiring a
more complete image of what he expects to see.
Good luck!
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477






----- Original Message -----
X-from: {Geoffrey_Williams-at-brown.edu}
To: {eikonika-at-otenet.gr}
Sent: Friday, March 27, 2009 7:44 PM

HI Geoff,

I notice you did not do the preparation. Are you sure the individuals who did are competent? There are lots of little nuances for each of several steps any one of which could result in trouble. Are they aware of and understand this or just cook booking through some protocol someone gave them- the gist of which looks sound by the way. Split prep in this case could be to your benefit allowing them to look over their protocols as opposed to anything you are doing on CPD

I have to agree with some of the other posts- this looks an awful lot like drying artifact but could easily go all the way back to growth conditions. Are they sure the absolute alcohol is absolute? Water exerts a tremendous amount of force and these cells seem to be pulling away from the substrate. Sieve with cupric sulfate indicator will prevent hydroscopic absorption. Mine needs recharging every 6 months or so in humidity and temp controlled museum- like clockwork 8 years now so I know something is up if it goes early. Same goes for the CO2 tanks. 99% of the time there is no need for the drying sieve but that 1% is still out there. I once ended up with the wrong grade CO2 and if not running through sieve would likely have contained traces of water blowing the samples. I now read the delivery ticket a little more carefully but nothing to say it couldn't happen again. Are they using different sample holders changing infiltration properties as ethanol is not very miscible with lCO2.

Even though you charge your users it might make sense for them to give you a set to prep yourself independently of them running the same set with the so called tried and true protocol and see what happens. If the expert is getting good cells to begin with then they will come out as such. Both of you come out with garbage...

Having seen posts from you in the past I suspected you covered the bases, but I decided this was a good opportunity for discussion and enlightenment at all experience levels. Troubleshooting tried and true protocols or methods can be a very time-consuming and frustrating experience if not properly prepared. In a case such as this I would concentrate most of my effort on what has changed since the last successful run, in particular things that could cause the cells to shrink- and no detail is too minute. A well run facility makes this easier as they mitigate change or uncertainty wherever possible. For instance where possible I try never to completely run out of any material or chemical and will begin using a new batch prior to the old running out. It's amazingly easy in a busy lab to move a decimal point and suddenly that 1x buffer is 10x. Something goes wrong and you have a more limited list of potential candidates and time to re-order or switch suppliers, which of course you have previously tried in the past and know will work equally well. It is not uncommon to order chemistry etc. which while having the same catalog number has changed manufacturers, materials, processing or whatever. I seem to recall an issue with glutaraldehyde recently on this list, and how many times have I seen Kodak cursed on this list over the years. Thankfully as an all digital facility I escaped those but it just served to reinforce maintenance of good lab management protocols and fallbacks.

Good luck,


Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891


-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Friday, March 27, 2009 12:09 PM
To: Whittaker, Scott

We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).

I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.

But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"

Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for).
Link to image (I have a few more images):
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg

I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.

Thanks!

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28 2009
9, 28 -- Received: from honeydew.services.brown.edu (honeydew.services.brown.edu [128.148.106.194])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RG6RN8014388
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 11:06:28 -0500
9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53])
9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3) with ESMTP id n2RG6PC6031327
9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 28 -- Content-class: urn:content-classes:message
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- Subject: SEM artifact help wanted
9, 28 -- Date: Fri, 27 Mar 2009 12:06:26 -0400
9, 28 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu}
9, 28 -- X-MS-Has-Attach:
9, 28 -- X-MS-TNEF-Correlator:
9, 28 -- Thread-Topic: SEM artifact help wanted
9, 28 -- Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA==
9, 28 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
9, 28 -- To: {microscopy-at-microscopy.com}
9, 28 -- X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC) FILETIME=[FB87A080:01C9AEF5]
9, 28 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 definitions=5565 signatures=515473
9, 28 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0903270105
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RG6RN8014388
==============================End of - Headers==============================


==============================Original Headers==============================
24, 29 -- From WHITTAKS-at-si.edu Fri Mar 27 15:10:59 2009
24, 29 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RKAw3i007479
24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:10:58 -0500
24, 29 -- Received: from si-msesmtpo-01.US.SINET.SI.EDU (SI-MSESMTP-N1.us.sinet.si.edu [160.111.49.75])
24, 29 -- by si-mailout03.si.edu (Postfix) with ESMTP id 48CE16AC3;
24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400 (EDT)
24, 29 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by si-msesmtpo-01.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959);
24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400
24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 29 -- Content-class: urn:content-classes:message
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Type: text/plain;
24, 29 -- charset="iso-8859-1"
24, 29 -- Subject: RE: [Microscopy] SEM artifact help wanted
24, 29 -- Date: Fri, 27 Mar 2009 16:10:52 -0400
24, 29 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA30395467F-at-SI-ECL02.US.SINET.SI.EDU}
24, 29 -- In-Reply-To: {200903271608.n2RG8v3m016738-at-ns.microscopy.com}
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- Thread-Topic: [Microscopy] SEM artifact help wanted
24, 29 -- Thread-Index: Acmu9leksGOaLsPSRCu4+GLAcLfdZQAD7XNQ
24, 29 -- References: {200903271608.n2RG8v3m016738-at-ns.microscopy.com}
24, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
24, 29 -- To: {microscopy-at-microscopy.com}
24, 29 -- Cc: {Geoffrey_Williams-at-brown.edu}
24, 29 -- X-OriginalArrivalTime: 27 Mar 2009 20:10:54.0056 (UTC) FILETIME=[21F52680:01C9AF18]
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RKAw3i007479
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Fri, 27 Mar 2009 15:42:01 -0500
Subject: [Microscopy] SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This may be a different angle...
There is a greater expansion of metal than there is of the biological
material, cells, when the sample is taken from Liquid CO2 temp through the
heated part of the CPD cycle past the critical temperature. Could HMDS be a
reasonable alternative to CPD?

A control could also be growing the same type cells on a glass coverslip to
be run through the CPD. A comparison would show only a difference of the
substrate. Titanium squares vs. glass coverslips.

Regards,
Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do
not represent the NIH. This message is not
confidential and can be freely shared and reproduced.

===
} From: {WHITTAKS-at-si.edu}
} Reply-To: {WHITTAKS-at-si.edu}
} Date: Fri, 27 Mar 2009 15:14:46 -0500
} To: {connellyps-at-nhlbi.nih.gov}
} Subject: [Microscopy] RE: SEM artifact help wanted
}
} HI Geoff,
}
} I notice you did not do the preparation. Are you sure the individuals who did
} are competent? There are lots of little nuances for each of several steps any
} one of which could result in trouble. Are they aware of and understand this or
} just cook booking through some protocol someone gave them- the gist of which
} looks sound by the way. Split prep in this case could be to your benefit
} allowing them to look over their protocols as opposed to anything you are
} doing on CPD
}
} I have to agree with some of the other posts- this looks an awful lot like
} drying artifact but could easily go all the way back to growth conditions. Are
} they sure the absolute alcohol is absolute? Water exerts a tremendous amount
} of force and these cells seem to be pulling away from the substrate. Sieve
} with cupric sulfate indicator will prevent hydroscopic absorption. Mine needs
} recharging every 6 months or so in humidity and temp controlled museum- like
} clockwork 8 years now so I know something is up if it goes early. Same goes
} for the CO2 tanks. 99% of the time there is no need for the drying sieve but
} that 1% is still out there. I once ended up with the wrong grade CO2 and if
} not running through sieve would likely have contained traces of water blowing
} the samples. I now read the delivery ticket a little more carefully but
} nothing to say it couldn't happen again. Are they using different sample
} holders changing infiltration properties as ethanol is not very miscible wit!
} h lCO2.
}
} Even though you charge your users it might make sense for them to give you a
} set to prep yourself independently of them running the same set with the so
} called tried and true protocol and see what happens. If the expert is getting
} good cells to begin with then they will come out as such. Both of you come out
} with garbage...
}
} Having seen posts from you in the past I suspected you covered the bases, but
} I decided this was a good opportunity for discussion and enlightenment at all
} experience levels. Troubleshooting tried and true protocols or methods can be
} a very time-consuming and frustrating experience if not properly prepared. In
} a case such as this I would concentrate most of my effort on what has changed
} since the last successful run, in particular things that could cause the cells
} to shrink- and no detail is too minute. A well run facility makes this easier
} as they mitigate change or uncertainty wherever possible. For instance where
} possible I try never to completely run out of any material or chemical and
} will begin using a new batch prior to the old running out. It's amazingly easy
} in a busy lab to move a decimal point and suddenly that 1x buffer is 10x.
} Something goes wrong and you have a more limited list of potential candidates
} and time to re-order or switch suppliers, which of course y!
} ou have previously tried in the past and know will work equally well. It is
} not uncommon to order chemistry etc. which while having the same catalog
} number has changed manufacturers, materials, processing or whatever. I seem to
} recall an issue with glutaraldehyde recently on this list, and how many times
} have I seen Kodak cursed on this list over the years. Thankfully as an all
} digital facility I escaped those but it just served to reinforce maintenance
} of good lab management protocols and fallbacks.
}
} Good luck,
}
}
} Scott Whittaker
} Head NMNH Imaging
} Manager SEM Lab
} Smithsonian Institution
} National Museum of Natural History
} PO Box 37012   MRC104
} Washington DC 20013-7012
} 202-633-0891
}
}
} -----Original Message-----
} X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
} Sent: Friday, March 27, 2009 12:09 PM
} To: Whittaker, Scott
} Subject: [Microscopy] SEM artifact help wanted
------------------------------------------------------------------------
}
} We have someone here who is trying to image cells grown on Titanium squares,
} both flat and micro-structured surfaces. They were getting decent results at
} one point and lately, or really the last month or two they have been chasing
} problems (as I understand the situation).
}
} I've looked over the protocol and it seems, to me, to be correct. The samples
} are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated
} and imaged.
}
} But without going into more details I'm just wondering if someone out here
} might be able to look at the image and say "Oh yeah, I know exactly what the
} problem is and how to fix it!"
}
} Hopefully though that solution doesn't involve high vacuum freeze drying or
} cryo-sem (neither of which we have the equipment for).
} Link to image (I have a few more images):
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg
}
} I am not personally doing the prep, but have been asked to see what can be
} done to help, esp since they are most interested in keeping the cellular
} projections intact. It may be a very simple issue but honestly maybe I've had
} too much botanical training in terms of sample prep and am missing it.
}
} Thanks!
}
} Geoff Williams
} Leduc Bioimaging Facility Manager
} Brown University
}  
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/
}
}

} ==============================Original Headers==============================
} 24, 29 -- From WHITTAKS-at-si.edu Fri Mar 27 15:10:59 2009
} 24, 29 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu
} [160.111.103.177])
} 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n2RKAw3i007479
} 24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:10:58 -0500
} 24, 29 -- Received: from si-msesmtpo-01.US.SINET.SI.EDU
} (SI-MSESMTP-N1.us.sinet.si.edu [160.111.49.75])
} 24, 29 -- by si-mailout03.si.edu (Postfix) with ESMTP id 48CE16AC3;
} 24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400 (EDT)
} 24, 29 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by
} si-msesmtpo-01.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959);
} 24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400
} 24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 24, 29 -- Content-class: urn:content-classes:message
} 24, 29 -- MIME-Version: 1.0
} 24, 29 -- Content-Type: text/plain;
} 24, 29 -- charset="iso-8859-1"
} 24, 29 -- Subject: RE: [Microscopy] SEM artifact help wanted
} 24, 29 -- Date: Fri, 27 Mar 2009 16:10:52 -0400
} 24, 29 -- Message-ID:
} {20CDD1CED2E76541A4FA119C6C806BA30395467F-at-SI-ECL02.US.SINET.SI.EDU}
} 24, 29 -- In-Reply-To: {200903271608.n2RG8v3m016738-at-ns.microscopy.com}
} 24, 29 -- X-MS-Has-Attach:
} 24, 29 -- X-MS-TNEF-Correlator:
} 24, 29 -- Thread-Topic: [Microscopy] SEM artifact help wanted
} 24, 29 -- Thread-Index: Acmu9leksGOaLsPSRCu4+GLAcLfdZQAD7XNQ
} 24, 29 -- References: {200903271608.n2RG8v3m016738-at-ns.microscopy.com}
} 24, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
} 24, 29 -- To: {microscopy-at-microscopy.com}
} 24, 29 -- Cc: {Geoffrey_Williams-at-brown.edu}
} 24, 29 -- X-OriginalArrivalTime: 27 Mar 2009 20:10:54.0056 (UTC)
} FILETIME=[21F52680:01C9AF18]
} 24, 29 -- Content-Transfer-Encoding: 8bit
} 24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n2RKAw3i007479
} ==============================End of - Headers==============================



==============================Original Headers==============================
9, 27 -- From connellyps-at-nhlbi.nih.gov Fri Mar 27 15:42:01 2009
9, 27 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RKfxES022249
9, 27 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:41:59 -0500
9, 27 -- X-IronPortListener: Outbound_SMTP
9, 27 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117])
9, 27 -- by nihxway2out.hub.nih.gov with ESMTP; 27 Mar 2009 16:41:58 -0400
9, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830);
9, 27 -- Fri, 27 Mar 2009 16:41:54 -0400
9, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.162]) with Microsoft Exchange Server HTTP-DAV ;
9, 27 -- Fri, 27 Mar 2009 20:41:53 +0000
9, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
9, 27 -- Date: Fri, 27 Mar 2009 16:40:31 -0400
9, 27 -- Subject: Re: [Microscopy] RE: SEM artifact help wanted
9, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov}
9, 27 -- To: {WHITTAKS-at-si.edu}
9, 27 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
9, 27 -- Message-ID: {C5F2B1FF.332A%connellyps-at-nhlbi.nih.gov}
9, 27 -- Thread-Topic: [Microscopy] RE: SEM artifact help wanted
9, 27 -- Thread-Index: AcmvHEUZg9tsRxsPEd6fYwANk2Yv1A==
9, 27 -- In-Reply-To: {200903272014.n2RKEkTJ014900-at-ns.microscopy.com}
9, 27 -- Mime-version: 1.0
9, 27 -- Content-type: text/plain;
9, 27 -- charset="ISO-8859-1"
9, 27 -- X-OriginalArrivalTime: 27 Mar 2009 20:41:54.0241 (UTC) FILETIME=[76B6DB10:01C9AF1C]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RKfxES022249
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 27 Mar 2009 15:49:18 -0500
Subject: [Microscopy] RE: SEM artifact help wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from time to time I am getting cell cultures of the same cell line, prepared by different people (graduate students, post docs.) Quality of specimens depends very much on a preparer. You can ask your researcher if somebody new in a lab prepared these cultures. The most common mistake is to let cells dry between steps of protocol. Keeping things simple, like dropping OsO4 (it is not really needed, glut alone is good enough for SEM) and switching from CPD to HMDS could help obtain more reproducible results.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Geoffrey_Williams-at-brown.edu
} [mailto:Geoffrey_Williams-at-brown.edu]
} Sent: Friday, March 27, 2009 11:08 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] SEM artifact help wanted
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} We have someone here who is trying to image cells grown on
} Titanium squares, both flat and micro-structured surfaces.
} They were getting decent results at one point and lately, or
} really the last month or two they have been chasing problems
} (as I understand the situation).
}
} I've looked over the protocol and it seems, to me, to be
} correct. The samples are chemically fixed (buffered Glute
} and OsO4), dehydrated (ETOH), CPD, coated and imaged.
}
} But without going into more details I'm just wondering if
} someone out here might be able to look at the image and say
} "Oh yeah, I know exactly what the problem is and how to fix it!"
}
} Hopefully though that solution doesn't involve high vacuum
} freeze drying or cryo-sem (neither of which we have the
} equipment for).
} Link to image (I have a few more images):
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_
} cell_problem.jpg
}
} I am not personally doing the prep, but have been asked to
} see what can be done to help, esp since they are most
} interested in keeping the cellular projections intact. It
} may be a very simple issue but honestly maybe I've had too
} much botanical training in terms of sample prep and am missing it.
}
} Thanks!
}
} Geoff Williams
} Leduc Bioimaging Facility Manager
} Brown University
}  
} http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/
}
}
}
} ==============================Original
} Headers==============================
} 9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28
} 2009 9, 28 -- Received: from honeydew.services.brown.edu
} (honeydew.services.brown.edu [128.148.106.194])
} 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n2RG6RN8014388
} 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar
} 2009 11:06:28 -0500
} 9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu
} (ex-gateway2.ad.brown.edu [128.148.21.53])
} 9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3)
} with ESMTP id n2RG6PC6031327
} 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar
} 2009 12:06:26 -0400
} 9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35])
} by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
} 9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400
} 9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 28
} -- Content-class: urn:content-classes:message 9, 28 --
} MIME-Version: 1.0 9, 28 -- Content-Type: text/plain;
} 9, 28 -- charset="iso-8859-1"
} 9, 28 -- Subject: SEM artifact help wanted 9, 28 -- Date:
} Fri, 27 Mar 2009 12:06:26 -0400 9, 28 -- Message-ID:
} {A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu}
} 9, 28 -- X-MS-Has-Attach:
} 9, 28 -- X-MS-TNEF-Correlator:
} 9, 28 -- Thread-Topic: SEM artifact help wanted 9, 28 --
} Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA== 9, 28 -- From:
} "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} 9, 28 --
} To: {microscopy-at-microscopy.com} 9, 28 --
} X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC)
} FILETIME=[FB87A080:01C9AEF5] 9, 28 --
} X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00
} definitions=5565 signatures=515473 9, 28 --
} X-Proofpoint-Spam-Details: rule=quarantine_notspam
} policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0
} bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx
} engine=5.0.0-0811170000 definitions=main-0903270105 9, 28 --
} Content-Transfer-Encoding: 8bit 9, 28 --
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n2RG6RN8014388
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Fri Mar 27 15:49:18 2009
7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RKnHLb003455
7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:49:18 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Fri, 27 Mar 2009 15:49:15 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="iso-8859-1"
7, 25 -- Subject: RE: [Microscopy] SEM artifact help wanted
7, 25 -- Date: Fri, 27 Mar 2009 15:49:14 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB80A-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200903271607.n2RG7awX015281-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] SEM artifact help wanted
7, 25 -- Thread-Index: Acmu9iZMZ576iX44TtSANclCodHjigAJz57g
7, 25 -- References: {200903271607.n2RG7awX015281-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {Geoffrey_Williams-at-brown.edu} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 27 Mar 2009 20:49:15.0372 (UTC) FILETIME=[7DA61AC0:01C9AF1D]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RKnHLb003455
==============================End of - Headers==============================




From: michelle.gignac-at-duke.edu
Date: Fri, 27 Mar 2009 18:45:48 -0500
Subject: [Microscopy] viaWWW: Ultracut Manual Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both michelle.gignac-at-duke.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: michelle.gignac-at-duke.edu
Name: Michelle Gignac

Organization: Duke University

Title-Subject: [Filtered] Ultracut Manual Needed

Question: Hello.
I have inherited an Ultracut microtome from another lab. It is
missing the manual. Does anyone have a pdf or hard copy they could
send me?

Thanks,
Michelle

Login Host: 152.3.194.20
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Mar 27 18:45:47 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RNjjr8022521
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 18:45:47 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c5f31599eac1-at-[206.69.208.22]}
7, 11 -- Date: Fri, 27 Mar 2009 18:45:44 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: michelle.gignac-at-duke.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Ultracut Manual Needed
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Lindsay.P.Keller-at-nasa.gov
Date: Sat, 28 Mar 2009 08:50:26 -0500
Subject: [Microscopy] viaWWW: Job opening: Electron Microscopy Scientist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Lindsay.P.Keller-at-nasa.gov as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Lindsay.P.Keller-at-nasa.gov
Name: Lindsay P. Keller

Organization: NASA

Title-Subject: [Filtered] Job opening: Electron Microscopy Scientist

Question:
Electron Microscopy Scientist
Description
The Astromaterials Research and Exploration Science Directorate
(ARES) at NASA's Johnson Space Center (JSC) is seeking a team member
with expertise in Scanning Electron Microscopy (SEM) to support
ongoing research and analysis projects. ARES is responsible for the
curation and analysis of a wide range of solar system materials
including the Apollo lunar samples, a large meteorite collection,
cosmic & comet dust samples (Stardust mission samples), samples from
the Sun (Genesis sample return mission), and space-exposed hardware.
Ongoing research includes a wide range of planetary, meteoritical,
and space exploration topics. ARES staff member backgrounds include
geology, chemistry, astronomy, physics, plus biology, mathematics,
computer science, and engineering.

We are searching for an individual with broad experience in scanning
electron microscopy to assist and train researchers and students in
the use of a field-emission SEM and a low-vacuum SEM instrument.
Typical analyses include secondary electron imaging, backscattered
electron imaging, low voltage work, and X-ray analysis and mapping
using energy-dispersive x-ray spectrometry. Responsibilities
include, but are not limited to: maintaining and operating all
aspects of these microscopes, coordinating service for the
instruments, and supporting peer-review research through high quality
analyses of astromaterials. Secondary responsibilities may include
development of new analytical techniques. In addition to the SEMs,
the facility also houses state-of-the art transmission electron
microscopes, and electron microprobe, and supporting instrumentation.

A bachelor's degree from an accredited university is a minimum
requirement; the degree should be in an applicable geoscience,
materials science, or engineering field. An advanced degree with
strong experience in one of these fields is highly preferred. Five
years of relevant experience is preferred. The candidate should have
a strong grasp of the theory and practice of scanning electron
microscopy analysis and data reduction. Experience with field
emission sources is a plus. Knowledge of geological and planetary
mineralogy is a plus, as is the ability to interpret analyses in a
geologically meaningful way. Good computer skills are essential.
Because the employee will often be working with students, and others
who may be unfamiliar with electron beam instruments, good people
skills are required. Must meet eligibility requirements to receive
and maintain a DoD security clearance (i.e., almost certainly needs
to be a US citizen).

For more information, please contact:

Lindsay P. Keller
Manager, Electron Beam Analysis Labs
Mail Code KR
NASA Johnson Space Center
Houston, TX 77058
Lindsay.P.Keller-at-nasa.gov



Login Host: 130.202.238.72
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Sat Mar 28 08:50:26 2009
12, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2SDoNIr024074
12, 11 -- for {microscopy-at-microscopy.com} ; Sat, 28 Mar 2009 08:50:25 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240802c5f3db8f5164-at-[206.69.208.22]}
12, 11 -- Date: Sat, 28 Mar 2009 08:50:22 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: Lindsay.P.Keller-at-nasa.gov (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Job opening: Electron Microscopy Scientist
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: peter.heimann-at-uni-bielefeld.de
Date: Sat, 28 Mar 2009 10:39:24 -0500
Subject: [Microscopy] RE: SEM artifact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Do you use WATERFREE CO2 for CPD-drying??
the standard carbon dioxide contains to my knowledge (at leat over here
in germany) considerable amount of water
after switching to (very expensive and certified ) water-free CO2 we
never again saw these cracks and ruptures in cell cuture cells in the
last 25 years.
In my experience the problem with water-free-CO2 never posed in tissue
or other "solid" organs...it seems to me, that (single) cell culture
cells are especially sensitive to even lowest content of water during CPD
as said in another posting drying of 100% ethanol (or in my case,
acetone) over molecular sieve helps a lot to avoid embedding or drying
problems.
good luck,
peter

====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107 33501 Bielefeld, Germany
www.uni-bielefeld.de/biologie/cellbio


==============================Original Headers==============================
4, 28 -- From peter.heimann-at-uni-bielefeld.de Sat Mar 28 10:39:23 2009
4, 28 -- Received: from mux2-unibi-smtp.hrz.uni-bielefeld.de (mux2-unibi-smtp.hrz.uni-bielefeld.de [129.70.204.73])
4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2SFdM7M009803
4, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 28 Mar 2009 10:39:23 -0500
4, 28 -- MIME-version: 1.0
4, 28 -- Content-transfer-encoding: 7BIT
4, 28 -- Content-type: text/plain; charset=ISO-8859-15; format=flowed
4, 28 -- Received: from pmxchannel-daemon.mux2-unibi-smtp.hrz.uni-bielefeld.de by
4, 28 -- mux2-unibi-smtp.hrz.uni-bielefeld.de
4, 28 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
4, 28 -- id {0KH8007003HLZS00-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} for
4, 28 -- Microscopy-at-microscopy.com; Sat, 28 Mar 2009 16:39:21 +0100 (CET)
4, 28 -- Received: from [192.168.2.102] ([84.135.243.157])
4, 28 -- by mux2-unibi-smtp.hrz.uni-bielefeld.de
4, 28 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
4, 28 -- with ESMTPPSA id {0KH8007ES3HHCE00-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} for
4, 28 -- Microscopy-at-microscopy.com; Sat, 28 Mar 2009 16:39:21 +0100 (CET)
4, 28 -- Date: Sat, 28 Mar 2009 16:39:30 +0100
4, 28 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de}
4, 28 -- Subject: RE: SEM artifact
4, 28 -- To: Microscopy-at-microscopy.com
4, 28 -- Message-id:
4, 28 -- {23752_1238254761_ZZg0q2T04iEIb.00_49CE44B2.2000605-at-uni-bielefeld.de}
4, 28 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de
4, 28 -- X-PMX-Version: 5.5.1.360522, Antispam-Engine: 2.6.1.350677,
4, 28 -- Antispam-Data: 2009.3.28.151921, pmx8
4, 28 -- X-Connecting-IP: 84.135.243.157
4, 28 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
==============================End of - Headers==============================




From: Jacqueline.Ayotte-at-ticona.com
Date: Mon, 30 Mar 2009 08:36:18 -0500
Subject: [Microscopy] OM - resolution rule of thumb?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

Is there a documented rule of thumb regarding minimum resolution per
pixel when measuring particles of a certain size range in an image? For
example, if one were interested in measuring particles down to 6 microns
would one set magnification to yield 3 microns per pixel or 2 microns
per pixel in the acquired image? (or less?)

Again, looking for documentation on this. I need to demonstrate proper
resolution to peers in Europe and it is not enough that I just "say so"
without any supportive data.

Thank you for any help or pointers.

Regards,
Jackie

Jacqueline Ayotte
Microscopist - Advanced Materials Characterization
Ticona
8040 Dixie Highway
Florence KY 41042
859-372-3139
fax 859-372-3184
jacqueline.ayotte-at-ticona.com
The information contained in this e-mail, and any attachments thereto,
is confidential and is intended only for use by the individual(s) and/or
entity named above. If you are not the intended recipient of this
e-mail, you are hereby notified that any dissemination, distribution or
copying of this communication or any disclosure of the contents of this
communication to others is strictly prohibited. If you have received
this communication in error, please notify the sender immediately by
replying to this e-mail. Please then delete the original including all
attachments and any copy of any e-mail and printout thereof.





==============================Original Headers==============================
11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009
11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com [64.18.2.215])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2UDZfAj023794
11, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 08:35:43 -0500
11, 27 -- Received: from source ([148.163.79.14]) by exprod7ob114.postini.com ([64.18.6.12]) with SMTP
11, 27 -- ID DSNKSdDKqlMnO5ROJzAcKdFGlbXDxz0tn+QU-at-postini.com; Mon, 30 Mar 2009 06:35:43 PDT
11, 27 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959);
11, 27 -- Mon, 30 Mar 2009 08:35:38 -0500
11, 27 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
11, 27 -- Mon, 30 Mar 2009 08:35:37 -0500
11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 27 -- Content-class: urn:content-classes:message
11, 27 -- MIME-Version: 1.0
11, 27 -- Content-Type: text/plain;
11, 27 -- charset="us-ascii"
11, 27 -- Subject: OM - resolution rule of thumb?
11, 27 -- Date: Mon, 30 Mar 2009 09:35:35 -0400
11, 27 -- Message-ID: {A2D45D1AE135A5418A4D38183241688F031C55-at-amfloxmbx1.SW.CZDS.BZ}
11, 27 -- X-MS-Has-Attach:
11, 27 -- X-MS-TNEF-Correlator:
11, 27 -- Thread-Topic: OM - resolution rule of thumb?
11, 27 -- thread-index: AcmxPGgKG21fsMlsS5umd5lrybpNhA==
11, 27 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com}
11, 27 -- To: {Microscopy-at-microscopy.com}
11, 27 -- X-OriginalArrivalTime: 30 Mar 2009 13:35:37.0995 (UTC) FILETIME=[6952ADB0:01C9B13C]
11, 27 -- Content-Transfer-Encoding: 8bit
11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2UDZfAj023794
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 30 Mar 2009 09:34:17 -0500
Subject: [Microscopy] Re: OM - resolution rule of thumb?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Jacqueline,

ASTM has a standard on computerized imaging system for counting carbon
black particles with a TEM. The standard (sorry I don't have the vol or
standard no.) recommends resolution per pixel related to magnification.
These values were based on a 1 meg camera.

hope this helps..
Frank




Jacqueline.Ayotte
-at-ticona.com
To
03/30/2009 09:59 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] OM - resolution rule
Jacqueline.Ayotte of thumb?
-at-ticona.com












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



Hello All,

Is there a documented rule of thumb regarding minimum resolution per
pixel when measuring particles of a certain size range in an image? For
example, if one were interested in measuring particles down to 6 microns
would one set magnification to yield 3 microns per pixel or 2 microns
per pixel in the acquired image? (or less?)

Again, looking for documentation on this. I need to demonstrate proper
resolution to peers in Europe and it is not enough that I just "say so"
without any supportive data.

Thank you for any help or pointers.

Regards,
Jackie

Jacqueline Ayotte
Microscopist - Advanced Materials Characterization
Ticona
8040 Dixie Highway
Florence KY 41042
859-372-3139
fax 859-372-3184
jacqueline.ayotte-at-ticona.com
The information contained in this e-mail, and any attachments thereto,
is confidential and is intended only for use by the individual(s) and/or
entity named above. If you are not the intended recipient of this
e-mail, you are hereby notified that any dissemination, distribution or
copying of this communication or any disclosure of the contents of this
communication to others is strictly prohibited. If you have received
this communication in error, please notify the sender immediately by
replying to this e-mail. Please then delete the original including all
attachments and any copy of any e-mail and printout thereof.





==============================Original
Headers==============================
11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009
11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com
[64.18.2.215])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n2UDZfAj023794
11, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009
08:35:43 -0500
11, 27 -- Received: from source ([148.163.79.14]) by
exprod7ob114.postini.com ([64.18.6.12]) with SMTP
11, 27 -- ID DSNKSdDKqlMnO5ROJzAcKdFGlbXDxz0tn+QU-at-postini.com;
Mon, 30 Mar 2009 06:35:43 PDT
11, 27 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by
smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959);
11, 27 -- Mon, 30 Mar 2009 08:35:38 -0500
11, 27 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by
amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
11, 27 -- Mon, 30 Mar 2009 08:35:37 -0500
11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 27 -- Content-class: urn:content-classes:message
11, 27 -- MIME-Version: 1.0
11, 27 -- Content-Type: text/plain;
11, 27 -- charset="us-ascii"
11, 27 -- Subject: OM - resolution rule of thumb?
11, 27 -- Date: Mon, 30 Mar 2009 09:35:35 -0400
11, 27 -- Message-ID:
{A2D45D1AE135A5418A4D38183241688F031C55-at-amfloxmbx1.SW.CZDS.BZ}
11, 27 -- X-MS-Has-Attach:
11, 27 -- X-MS-TNEF-Correlator:
11, 27 -- Thread-Topic: OM - resolution rule of thumb?
11, 27 -- thread-index: AcmxPGgKG21fsMlsS5umd5lrybpNhA==
11, 27 -- From: "Ayotte, Jacqueline M., Ticona/US"
{Jacqueline.Ayotte-at-ticona.com}
11, 27 -- To: {Microscopy-at-microscopy.com}
11, 27 -- X-OriginalArrivalTime: 30 Mar 2009 13:35:37.0995 (UTC) FILETIME=
[6952ADB0:01C9B13C]
11, 27 -- Content-Transfer-Encoding: 8bit
11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2UDZfAj023794
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
30, 22 -- From frank_karl-at-lincolnelectric.com Mon Mar 30 09:34:16 2009
30, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
30, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UEYGRq007609
30, 22 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 09:34:16 -0500
30, 22 -- In-Reply-To: {200903301359.n2UDxmhZ005041-at-ns.microscopy.com}
30, 22 -- Subject: Re: [Microscopy] OM - resolution rule of thumb?
30, 22 -- To: Jacqueline.Ayotte-at-ticona.com, Microscopy-at-microscopy.com
30, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
30, 22 -- Message-ID: {OF121A7C77.B405EEAB-ON85257589.004F5FFD-85257589.005001F7-at-lincolnelectric.com}
30, 22 -- Date: Mon, 30 Mar 2009 10:33:58 -0400
30, 22 -- From: Frank_Karl-at-lincolnelectric.com
30, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM,
30, 22 -- CD-MIME complete at 03/30/2009 10:33:56 AM,
30, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM,
30, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM,
30, 22 -- Serialize complete at 03/30/2009 10:33:56 AM
30, 22 -- MIME-Version: 1.0
30, 22 -- Content-Type: text/plain;
30, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: hale0007-at-mc.duke.edu
Date: Mon, 30 Mar 2009 13:42:57 -0500
Subject: [Microscopy] EM Tech Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Technician Position Available

Location: Duke University Medical Center, Durham, NC

Requirements: BS degree. US citizen or green card. Prior training and
experience in running electron microscopes, proficiency in cutting
ultrathin
sections and performing negative staining. Knowledge of scientific
laboratory
operation (making solutions, ordering supplies, typing results, keeping
records, etc.). Clinical laboratory and research experience is
advantageous.

Laboratory description: The work force consists of the director and 6 EM
technologists who perform pathology (500 samples/year), virology (1000
samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes?2 with
cryo attachments, plus ancillary specimen preparation equipment.

EM Laboratory web site:
http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain

Send resume to:
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Laboratory
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Phone: 919 684-3452
Fax: 919 684-3265
Email: saram-at-duke.edu



==============================Original Headers==============================
9, 26 -- From hale0007-at-mc.duke.edu Mon Mar 30 13:42:56 2009
9, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UIgt03031599
9, 26 -- for {Microscopy-at-microscopy.org} ; Mon, 30 Mar 2009 13:42:56 -0500
9, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54])
9, 26 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n2UIgpP92248860
9, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
9, 26 -- for {Microscopy-at-microscopy.org} ; Mon, 30 Mar 2009 14:42:52 -0400
9, 26 -- Importance: Normal
9, 26 -- X-Priority: 3 (Normal)
9, 26 -- In-Reply-To:
9, 26 -- References:
9, 26 -- Subject: EM Tech Position Available
9, 26 -- MIME-Version: 1.0
9, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu}
9, 26 -- To: Microscopy-at-microscopy.org
9, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 14:42:47,
9, 26 -- MIME-CD complete at 03/30/2009 14:42:48,
9, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 02:42:51 PM
9, 26 -- Message-ID: {OFAE1A9166.74E35CBD-ON85257589.0066CB8E-85257589.0066CBF5-at-notes.duke.edu}
9, 26 -- Date: Mon, 30 Mar 2009 14:42:48 -0400
9, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008
9, 26 -- Content-type: text/plain; charset=US-ASCII
9, 26 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201
==============================End of - Headers==============================




From: hale0007-at-mc.duke.edu
Date: Mon, 30 Mar 2009 13:48:00 -0500
Subject: [Microscopy] EM Tech Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Technician Position Available

Location: Duke University Medical Center, Durham, NC

Requirements: BS degree. US citizen or green card. Prior training and
experience in running electron microscopes, proficiency in cutting
ultrathin
sections and performing negative staining. Knowledge of scientific
laboratory
operation (making solutions, ordering supplies, typing results, keeping
records, etc.). Clinical laboratory and research experience is
advantageous.

Laboratory description: The work force consists of the director and 6 EM
technologists who perform pathology (500 samples/year), virology (1000
samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes?2 with
cryo attachments, plus ancillary specimen preparation equipment.

EM Laboratory web site:
http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain

Send resume to:
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Laboratory
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Phone: 919 684-3452
Fax: 919 684-3265
Email: saram-at-duke.edu



==============================Original Headers==============================
9, 26 -- From hale0007-at-mc.duke.edu Mon Mar 30 13:48:00 2009
9, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UIlwhw004946
9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 13:47:59 -0500
9, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54])
9, 26 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n2UIlumC995436
9, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 14:47:56 -0400
9, 26 -- Importance: Normal
9, 26 -- X-Priority: 3 (Normal)
9, 26 -- In-Reply-To:
9, 26 -- References:
9, 26 -- Subject: EM Tech Position Available
9, 26 -- MIME-Version: 1.0
9, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu}
9, 26 -- To: Microscopy-at-microscopy.com
9, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 14:47:51,
9, 26 -- MIME-CD complete at 03/30/2009 14:47:51,
9, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release
9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 02:47:55 PM
9, 26 -- Message-ID: {OFE9DBC44C.036B659D-ON85257589.00674231-85257589.0067423D-at-notes.duke.edu}
9, 26 -- Date: Mon, 30 Mar 2009 14:47:51 -0400
9, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008
9, 26 -- Content-type: text/plain; charset=US-ASCII
9, 26 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201
==============================End of - Headers==============================




From: samwarren-at-emfocal.com
Date: Mon, 30 Mar 2009 15:20:05 -0500
Subject: [Microscopy] TEM - New info about emfocal.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,
Thank you for all your comments, either positive or negative. They sure
help me in reshaping the site.
I am sorry that I rushed my previous messages in trying to get a quick
response to your comments. They were done in a Chinese internet bar (with
a beer and many kids playing games around me) when I was on vacation in
China. If they sound like unprofessional and too informal, I am sorry
about that.
Now about me:
Shixin Wang
BS in Physics at Beijing Polytechnic University, Beijing, China, 1986.
Ph.D in Earth and Planetary Science at University of New Mexico, 1997.
Post-doc fellow on Radiation effect and TEM at University of Michigan,
1997-2000.
Senior Engineer, Micron Technology Inc., Boise, Idaho, 2000 - present.

I started on TEM training on 1992 when I started at Univ of New Mexico.
and used TEM and related techniques ever since. Now in TEM laboratory at
Micron Technology for 9 years, I consider myself a TEM professional.

I started this site: http://www.emfocal.com about a month ago. I
registered a company "Emfocal Inc" for this site. Reason? (1) Separating
myself from any possible legal issues; (2) Making it a non-personal site;
and (3) It can be passed over to other generations.

The primary function of our site is an on-line publication platform. There
are wiki-books, forums, article, FAQ, Image gallery, resume, etc. It is a
public service site. I do reserve the right to put some advertisements on
the site to gain income. The site is user interactive and dynamic, you can
post, modify, or delete you post. When the site grows, some users can
become site moderators or administrators.

It is hosted at hostmonster.com. It is a cheap hosting, but offers
unlimited storage use. So far, it suits my needs. When the site grows in
traffic, it may need to be moved to a dedicated (more expensive) server.

The site is not redundant as to this listserver. For one thing, there are
wiki-book and article publications. If you have lecture notes, tutorials,
you may want to publish them there to benefit more people. That site will
not prevent you to publish your work again at other place. After I get
clearance from my employer, I will post some of my stuff on. If you want
to post resume or job posting specifically for the EM field, that is a
place to go.

The "terms of use" was not clear enough for professional use. I will
rewrite it and make it simple.

Your comments and participations are always welcome.

Thanks,

Sam


==============================Original Headers==============================
11, 31 -- From samwarren-at-emfocal.com Mon Mar 30 15:20:05 2009
11, 31 -- Received: from outbound-mail-358.bluehost.com (outbound-mail-358.bluehost.com [66.147.249.252])
11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2UKK5DL029980
11, 31 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Mar 2009 15:20:05 -0500
11, 31 -- Received: (qmail 18071 invoked by uid 0); 30 Mar 2009 20:14:55 -0000
11, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161)
11, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 30 Mar 2009 20:14:55 -0000
11, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com;
11, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
11, 31 -- b=ayWqzUzPbbxlXwf/pcc65zcyY5ygSsCbaE6oNj2eVDGPZ4Y+CGbv6LUt+Rm0zCCe4kgZfjAfwZpl3LSJ66s3I/L47+Mmi2tVM1JQ6DPzv4CN5NEM9iLNAwW+smbgmKwT;
11, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com)
11, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69)
11, 31 -- (envelope-from {samwarren-at-emfocal.com} )
11, 31 -- id 1LoNxi-0007qs-2H
11, 31 -- for Microscopy-at-Microscopy.Com; Mon, 30 Mar 2009 14:20:02 -0600
11, 31 -- Received: from 208.100.250.244 ([208.100.250.244])
11, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com)
11, 31 -- by host361.hostmonster.com with HTTP;
11, 31 -- Mon, 30 Mar 2009 14:20:02 -0600 (MDT)
11, 31 -- Message-ID: {2565.208.100.250.244.1238444402.squirrel-at-host361.hostmonster.com}
11, 31 -- Date: Mon, 30 Mar 2009 14:20:02 -0600 (MDT)
11, 31 -- Subject: TEM - New info about emfocal.com
11, 31 -- From: samwarren-at-emfocal.com
11, 31 -- To: Microscopy-at-Microscopy.Com
11, 31 -- User-Agent: SquirrelMail/1.4.13
11, 31 -- MIME-Version: 1.0
11, 31 -- Content-Type: text/plain;charset=iso-8859-1
11, 31 -- Content-Transfer-Encoding: 8bit
11, 31 -- X-Priority: 3 (Normal)
11, 31 -- Importance: Normal
11, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server}
==============================End of - Headers==============================




From: osborndc-at-umsl.edu
Date: Mon, 30 Mar 2009 19:31:24 -0500
Subject: [Microscopy] TEM/SEM Analysis of Liposomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need help in analyzing 200-300nm liposomes using TEM and SEM. What's the best way to prepare a suspension of liposomes for size analysis? Any suggestions/recommendations would be appreciated.




==============================Original Headers==============================
2, 23 -- From osborndc-at-umsl.edu Mon Mar 30 19:31:24 2009
2, 23 -- Received: from stl-proto10.umsl.edu (STL-PROTO10.umsl.edu [134.124.45.28])
2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V0VNJt019267
2, 23 -- for {Microscopy-at-Microscopy.com} ; Mon, 30 Mar 2009 19:31:24 -0500
2, 23 -- Received: from stl-mail4.stl.umsl.edu ([134.124.45.88]) by stl-proto10.umsl.edu with Microsoft SMTPSVC(6.0.3790.3959);
2, 23 -- Mon, 30 Mar 2009 19:31:20 -0500
2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
2, 23 -- Content-class: urn:content-classes:message
2, 23 -- MIME-Version: 1.0
2, 23 -- Content-Type: text/plain;
2, 23 -- charset="iso-8859-1"
2, 23 -- Subject: TEM/SEM Analysis of Liposomes
2, 23 -- Date: Mon, 30 Mar 2009 19:31:20 -0500
2, 23 -- Message-ID: {27F0D02F0DB26944944521116306B3DA03D61670-at-stl-mail4.stl.umsl.edu}
2, 23 -- X-MS-Has-Attach:
2, 23 -- X-MS-TNEF-Correlator:
2, 23 -- Thread-Topic: TEM/SEM Analysis of Liposomes
2, 23 -- Thread-Index: AcmxmANHVl+YwN2pT1Cq6I/EOKx54w==
2, 23 -- From: "Osborn, David C." {osborndc-at-umsl.edu}
2, 23 -- To: {Microscopy-at-Microscopy.com}
2, 23 -- X-OriginalArrivalTime: 31 Mar 2009 00:31:20.0989 (UTC) FILETIME=[039324D0:01C9B198]
2, 23 -- Content-Transfer-Encoding: 8bit
2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2V0VNJt019267
==============================End of - Headers==============================




From: jrminter-at-rochester.rr.com
Date: Mon, 30 Mar 2009 20:58:30 -0500
Subject: [Microscopy] TEM/SEM Analysis of Liposomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have examined liposome suspensions by cryo-TEM in vitreous ice. You should
keep in mind that in this, as other techniques, the liposomes are confined
to a thin film. Flattening is very likely. This will most likely bias your
size distribution measurements from projected area. In such cases we look at
the apparent polydispersity and then use a complementary technique, such as
dynamic light scattering to study the specimen.

-----Original Message-----
X-from: osborndc-at-umsl.edu [mailto:osborndc-at-umsl.edu]
Sent: Monday, March 30, 2009 8:32 PM
To: jrminter-at-rochester.rr.com

I need help in analyzing 200-300nm liposomes using TEM and SEM. What's the
best way to prepare a suspension of liposomes for size analysis? Any
suggestions/recommendations would be appreciated.




==============================Original Headers==============================
2, 23 -- From osborndc-at-umsl.edu Mon Mar 30 19:31:24 2009
2, 23 -- Received: from stl-proto10.umsl.edu (STL-PROTO10.umsl.edu
[134.124.45.28])
2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n2V0VNJt019267
2, 23 -- for {Microscopy-at-Microscopy.com} ; Mon, 30 Mar 2009 19:31:24
-0500
2, 23 -- Received: from stl-mail4.stl.umsl.edu ([134.124.45.88]) by
stl-proto10.umsl.edu with Microsoft SMTPSVC(6.0.3790.3959);
2, 23 -- Mon, 30 Mar 2009 19:31:20 -0500
2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
2, 23 -- Content-class: urn:content-classes:message
2, 23 -- MIME-Version: 1.0
2, 23 -- Content-Type: text/plain;
2, 23 -- charset="iso-8859-1"
2, 23 -- Subject: TEM/SEM Analysis of Liposomes
2, 23 -- Date: Mon, 30 Mar 2009 19:31:20 -0500
2, 23 -- Message-ID:
{27F0D02F0DB26944944521116306B3DA03D61670-at-stl-mail4.stl.umsl.edu}
2, 23 -- X-MS-Has-Attach:
2, 23 -- X-MS-TNEF-Correlator:
2, 23 -- Thread-Topic: TEM/SEM Analysis of Liposomes
2, 23 -- Thread-Index: AcmxmANHVl+YwN2pT1Cq6I/EOKx54w==
2, 23 -- From: "Osborn, David C." {osborndc-at-umsl.edu}
2, 23 -- To: {Microscopy-at-Microscopy.com}
2, 23 -- X-OriginalArrivalTime: 31 Mar 2009 00:31:20.0989 (UTC)
FILETIME=[039324D0:01C9B198]
2, 23 -- Content-Transfer-Encoding: 8bit
2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n2V0VNJt019267
==============================End of - Headers==============================
No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 8.5.285 / Virus Database: 270.11.33/2031 - Release Date: 03/30/09
17:56:00


==============================Original Headers==============================
9, 22 -- From jrminter-at-rochester.rr.com Mon Mar 30 20:58:30 2009
9, 22 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V1wUwx007088
9, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Mar 2009 20:58:30 -0500
9, 22 -- Received: from parrotxp ([67.240.238.57]) by hrndva-omta04.mail.rr.com
9, 22 -- with ESMTP
9, 22 -- id {20090331015827.RWPM15546.hrndva-omta04.mail.rr.com-at-parrotxp}
9, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 31 Mar 2009 01:58:27 +0000
9, 22 -- From: "John Minter" {jrminter-at-rochester.rr.com}
9, 22 -- To: {Microscopy-at-Microscopy.Com}
9, 22 -- References: {200903310031.n2V0VXQe019397-at-ns.microscopy.com}
9, 22 -- In-Reply-To: {200903310031.n2V0VXQe019397-at-ns.microscopy.com}
9, 22 -- Subject: RE: [Microscopy] TEM/SEM Analysis of Liposomes
9, 22 -- Date: Mon, 30 Mar 2009 21:58:19 -0400
9, 22 -- Message-ID: {000001c9b1a4$2ff46af0$8fdd40d0$-at-rr.com}
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain;
9, 22 -- charset="us-ascii"
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-Mailer: Microsoft Office Outlook 12.0
9, 22 -- Thread-Index: AcmxmAyFBIQrVVT+Tg+79RJf8fHTCwACxZLA
9, 22 -- Content-Language: en-us
==============================End of - Headers==============================




From: hmendlowitz-at-gmail.com
Date: Mon, 30 Mar 2009 23:43:31 -0500
Subject: [Microscopy] viaWWW: Trinocular adapter for Ernst Leitz Wetzler scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hmendlowitz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hmendlowitz-at-gmail.com
Name: Harriet Mendlowitz

Organization: Photo Researchers Inc

Title-Subject: [Filtered] Trinocular adapter for Ernst Leitz Wetzler scopes

Question: Does anyone know whether all Labolux & SM-D Lux scopes will
take a trinocular adapter? I am specifically interested in a model
from the sixties, serial #674661.
Many thanks.

Login Host: 216.220.108.222
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Mar 30 23:43:30 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V4hTeN025677
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 23:43:30 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5f74fe09878-at-[206.69.208.22]}
6, 11 -- Date: Mon, 30 Mar 2009 23:43:27 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: hmendlowitz-at-gmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Trinocular adapter for Ernst Leitz Wetzler scopes
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hmendlowitz-at-gmail.com
Date: Mon, 30 Mar 2009 23:44:00 -0500
Subject: [Microscopy] viaWWW: Olympus CH series

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hmendlowitz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hmendlowitz-at-gmail.com
Name: Harriet Mendlowitz

Organization: Photo Researchers Inc

Title-Subject: [Filtered] Olympus CH series

Question: Does anyone know if the Olympus CH series (CHA,
specifically) made during the 70's accepts trinocular adapters?

Login Host: 216.220.108.222
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Mar 30 23:44:00 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V4hump026175
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 23:43:58 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c5f74ffb9e98-at-[206.69.208.22]}
6, 11 -- Date: Mon, 30 Mar 2009 23:43:55 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: hmendlowitz-at-gmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Olympus CH series
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: yolande.berta-at-mse.gatech.edu
Date: Mon, 30 Mar 2009 23:44:32 -0500
Subject: [Microscopy] viaWWW: Job Opening: Engineer for TEM and SEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yolande.berta-at-mse.gatech.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yolande.berta-at-mse.gatech.edu
Name: Yolande Berta

Organization: Georgia Institute of Technology

Title-Subject: [Filtered] Job Opening: Engineer for TEM and SEM service

Question: The Center for Nanostructure Characterization and The
School of Materials Science and Engineering, at the Georgia Institute
of Technology, seek an electrical Engineer.

Duties: Maintain and repair transmission electron microscopes (JEOL
100CX-II, Hitachi HF-2000, JEOL 4000EX), scanning electron
microscopes (LEO 1530, LEO 1550, Hitachi S-800), and specimen
preparation equipment (ion mill, PIPS, and ultramicrotome); train and
interact with users on the use of the equipment. Other duties as
required such as testing of equipment, etc. Hours may vary depending
on machine status.

---------------
Qualifications: Bachelor's Degree in Electrical Engineering or
related field, or equivalent combination of education and experience.
Prefer a Master's Degree.

Experience: Minimum of four years of work related experience in
engineering preferred; Proven knowledge and experience with
transmission and scanning electron microscopes

Must be able to work independently using sound judgment, and must
possess excellent work ethic. Selection process will include a
pre-employment background screening.

The Georgia Institute of Technology is an Equal Opportunity Employer.

Contact: zhong.wang-at-mse.gatech.edu or submit via our online
application system at https://ea.ohr.gatech.edu/careers/

Submitted by:
Yolande Berta
Georgia Tech


Login Host: 143.215.239.154
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Mon Mar 30 23:44:31 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V4iVME027838
14, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 23:44:31 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240802c5f75019a5cc-at-[206.69.208.22]}
14, 11 -- Date: Mon, 30 Mar 2009 23:44:30 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: yolande.berta-at-mse.gatech.edu (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: Job Opening: Engineer for TEM and SEM service
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 31 Mar 2009 00:19:55 -0500
Subject: [Microscopy] Re: viaWWW: Olympus CH series

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes they do if you have the trinocular head.

The neat thing about the early CH and BH is that they
mostly interchangeable between parts--outside of DIC and
phase.

Ought not be a big problem. What is the issue?

Dr. Gary Gaugler

At 09:45 PM 3/30/2009, you wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
6, 20 -- From gary-at-gaugler.com Tue Mar 31 00:19:55 2009
6, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2V5JtgN002990
6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 00:19:55 -0500
6, 20 -- Message-Id: {200903310519.n2V5JtgN002990-at-ns.microscopy.com}
6, 20 -- Received: (qmail 13119 invoked from network); 30 Mar 2009 22:27:58 -0700
6, 20 -- Received: by simscan 1.1.0 ppid: 13116, pid: 13117, t: 0.0785s
6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
6, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
6, 20 -- by smtp1 with SMTP; 30 Mar 2009 22:27:58 -0700
6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
6, 20 -- Date: Mon, 30 Mar 2009 22:19:52 -0700
6, 20 -- To: hmendlowitz-at-gmail.com
6, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
6, 20 -- Subject: Re: [Microscopy] viaWWW: Olympus CH series
6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
6, 20 -- In-Reply-To: {200903310445.n2V4jnAk032335-at-ns.microscopy.com}
6, 20 -- References: {200903310445.n2V4jnAk032335-at-ns.microscopy.com}
6, 20 -- Mime-Version: 1.0
6, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 31 Mar 2009 00:23:34 -0500
Subject: [Microscopy] Re: viaWWW: Olympus CH series

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve...what is going on in your organization about
microphoto trivia?

This is odd.

gary g.


---------------------------------------------------

Yes they do if you have the trinocular head.

The neat thing about the early CH and BH is that they
mostly interchangeable between parts--outside of DIC and
phase.

Ought not be a big problem. What is the issue?

Dr. Gary Gaugler

At 09:45 PM 3/30/2009, you wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 18 -- From gary-at-gaugler.com Tue Mar 31 00:23:34 2009
11, 18 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2V5NYE1010868
11, 18 -- for {microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 00:23:34 -0500
11, 18 -- Message-Id: {200903310523.n2V5NYE1010868-at-ns.microscopy.com}
11, 18 -- Received: (qmail 18604 invoked from network); 30 Mar 2009 22:20:58 -0700
11, 18 -- Received: by simscan 1.1.0 ppid: 18601, pid: 18602, t: 0.0821s
11, 18 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 18 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 18 -- by smtp2 with SMTP; 30 Mar 2009 22:20:57 -0700
11, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 18 -- Date: Mon, 30 Mar 2009 22:23:31 -0700
11, 18 -- To: steve-at-photoresearchers.com
11, 18 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 18 -- Subject: Re: [Microscopy] viaWWW: Olympus CH series
11, 18 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 18 -- Mime-Version: 1.0
11, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: elena.belluso-at-unito.it
Date: Tue, 31 Mar 2009 06:05:18 -0500
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear friend

We have prepared for SEM liposomes and other nanoparticles by placing them
on a filter paper. Please have a look at this publication:
Scanning electron microscopy study on nanoemulsions and solid lipid
nanoparticles containing high amounts of ceramides.

Hatziantoniou S, Deli G, Nikas Y, Demetzos C, Papaioannou GT.

Micron. 2007;38(8):819-23. Epub 2007 Jul 3.

Regards

yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477

----- Original Message -----
X-from: {osborndc-at-umsl.edu}
To: {eikonika-at-otenet.gr}
Sent: Tuesday, March 31, 2009 3:37 AM

Nell’ambito del VII° Forum della FIST - Geoitalia2009 (Rimini 9-11
settembre 2009; http://www.geoitalia.org/), stiamo organizzando la
sessione tematica D7 “Il particolato minerale: origine, aspetti
cristallochimici, risvolti ambientali e sanitari” e il corso breve SC 3
“Particolato minerale: origine, campionamento, analisi, potenzialità di
inquinamento e di rischio, risanamento”.


La sessione D7 si prefigge di fare il punto sulle conoscenze relative al
particolato minerale nelle varie frazioni dimensionali e nei vari ambienti
di origine e/o di recupero, tenendo in considerazione che polveri sottili,
particolato e fibre minerali presentano ormai da anni grande interesse ed
attenzione e che, comunque, determinati aspetti del problema devono essere
ancora affrontati ed interpretati. Saranno pertanto considerate e discusse
le attuali metodologie di campionamento, la caratterizzazione mineralogica
sia delle fasi primarie sia di quelle secondarie, le interazioni con
l’ambiente e con gli esseri umani, le cause di aero-dispersione, le
possibili soluzioni per la prevenzione e il risanamento.

Il corso breve SC 3 verterà sui temi della sessione D7, puntando in
particolare sugli aspetti analitici, tossicologici e normativi della
problematica.
Il corso è rivolto a ricercatori, studiosi ed operatori del settore.

Con l’obiettivo di scambiare informazioni sullo stato delle conoscenze e
di incentivare collaborazioni interdisciplinari, Vi invitiamo ad inviare
contributi orali e/o poster per la sessione D7 inerenti i vari aspetti del
“particolato minerale”.

Il termine ultimo per l’invio dei riassunti è il 16 maggio 2009.

Scusandoci per invii multipli, inviamo cordiali saluti,
Elena Belluso (elena.belluso-at-unito.it)
Antonio GIANFAGNA (antonio.gianfagna-at-uniroma1.it)
Alessandro GUALTIERI (alessandro.gualtieri-at-unimore.it)

---------------------------------------------------------------------------------------------------------------------
(we apologize for multiple postings)

In the context of the 7th Forum Geoitalia 2009 (9-10-11 September 2009,
http://www.geoitalia.org) we are organizing the D7 thematic session titled
“Mineral particulate: origin, crystal chemical aspects, and related
environmental and health issues”, and the SC 3 short course “Mineral
particulate: origin, sampling, analyses, pollution and risk potentiality,
reclamation”.

The D7 thematic session aims to fix the actual knowledge related to the
particulate mineral, the various dimensional fractions, and the different
environments of origin and recovering, taking into account that thin
dusts, particulate, and mineral fibers have given rise to particular
attention and interest for many years, but specific aspects of their
interactions with different environments still need to be explored and
understood.

Sampling methodologies and mineralogical characterization of the primary
and secondary mineral phases will be considered. Moreover, the
interactions between the environment and humans, the causes of the
air-dispersion, and the possible solutions for prevention and reclamation
will also be subject of debate.

The SC 3 short course is strongly correlated to the D7 thematic session
and aims at developing specific arguments on the analysis, toxicology and
Italian regulations on the mineral particulate issue.
The course is addressed to researchers, scientists, and people operating
in this topic.

In order to exchange information regarding the actual knowledge and to
increase interdisciplinary collaborations, we invite you to send
contributions (talks and posters) about the different topics of the
“mineral particulate” for the D7 thematic session

Note the abstract deadline is May 16, 2009.

Best regards,

the conveners
Elena Belluso (elena.belluso-at-unito.it)
Antonio GIANFAGNA (antonio.gianfagna-at-uniroma1.it)
Alessandro GUALTIERI (alessandro.gualtieri-at-unimore.it)


-----------------------------------------------------------------------------------
Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography
Dipartimento di Scienze Mineralogiche e Petrologiche
Universita' degli Studi di Torino
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28
e-mail: elena.belluso-at-unito.it
http://www.dsmp.unito.it
-----------------------------------------------------------------------------------
"I've... seen things you people wouldn't believe.
Attack ships on fire off the shoulder of Orion.
I watched C-beams... glitter in the dark near the Tanhauser Gate.
All those... moments will be lost... in time...,
like... tears... in... rain."
Blade Runner





==============================Original Headers==============================
22, 99 -- From elena.belluso-at-unito.it Tue Mar 31 06:04:47 2009
22, 99 -- Received: from mail-out.unito.it (opterone.unito.it [130.192.119.88])
22, 99 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VB4kFR022193
22, 99 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 06:04:46 -0500
22, 99 -- Received: (from root-at-localhost)
22, 99 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) id n2VB4iNE027755
22, 99 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 13:04:44 +0200
22, 99 -- Received: from mail.unito.it (giove.unito.it [130.192.119.45])
22, 99 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) with SMTP id n2VB25A6020726;
22, 99 -- Tue, 31 Mar 2009 13:02:05 +0200
22, 99 -- X-Icontrol: Sent by Inrete Icontrol
22, 99 -- Received: from 130.192.111.68
22, 99 -- (SquirrelMail authenticated user ebelluso-at-unito.it)
22, 99 -- by mail.unito.it with HTTP;
22, 99 -- Tue, 31 Mar 2009 13:02:05 +0200 (CEST)
22, 99 -- Message-ID: {2510.130.192.111.68.1238497325.squirrel-at-mail.unito.it}
22, 99 -- Date: Tue, 31 Mar 2009 13:02:05 +0200 (CEST)
22, 99 -- Subject:
22, 99 -- From: elena.belluso-at-unito.it
22, 99 -- To: phil-at-aarq.com.au, office-at-allcoastdemolition.com.au,
22, 99 -- asbestos-at-iprimus.com.au, jedwards-at-atstech.com.au, office-at-campak.com.au,
22, 99 -- andrew-at-collettplumbingandroofing.com.au, ahmf-at-conversegroup.com.au,
22, 99 -- dtedemo-at-bigpond.com, dalcott-at-bigpond.net.au, don-at-imagecom.com.au,
22, 99 -- irtaust-at-bigpond.net.au, rbary-at-gil.com.au, wayne.p-at-austarnet.com.au,
22, 99 -- martina-at-mcmahon-services.com.au, bunneys_demolition-at-bigpond.com.au,
22, 99 -- pat-at-ordanga.com.au, James-at-roofrite.com.au, d.swainson-at-ttmetal.com.au,
22, 99 -- z.vanthavong-at-aerify.com, srp-at-gil.com.au, bsch-at-bigpond.com.au,
22, 99 -- gzealand-at-coastalnet.com.au, brisbane-at-heggies.com,
22, 99 -- wade.russell-at-noel-arnold.com.au, mshepherd-at-pb.com.au,
22, 99 -- inspections-at-qbm.com.au, gavin.irving-at-dme.qld.gov.au,
22, 99 -- mark-at-tilecoat.com.au, steve-at-keyshr.com, cdcinfo-at-cdc.gov,
22, 99 -- robbins.katherine-at-epa.gov, wsweet-at-cdc.gov, gperlman-at-cdc.gov,
22, 99 -- ablock-at-cdc.gov, Blonk.Jonathan-at-epa.gov, Escobar.Leah-at-epa.gov,
22, 99 -- jones.steve-at-epa.gov, somers.tarah-at-epa.gov, Muza.Susan-at-epamail.epa.gov,
22, 99 -- geng-at-cdc.gov, vianu.libby-at-epa.gov, Borg.Patricia-at-cdc.gov,
22, 99 -- dstrausbaugh-at-cdc.gov, cpoulet-at-cdc.gov, gtucker-at-cdc.gov,
22, 99 -- Markiewicz.Karl-at-epa.gov, bbecerra-at-cdc.gov, patrickyoung-at-cdc.gov,
22, 99 -- bbecerra-at-cdc.gov, jlyke-at-cdc.gov, bsamuels-at-cdc.gov, cblair-at-cdc.gov,
22, 99 -- benmoore-at-cdc.gov, rsafay-at-cdc.gov, bruce.case-at-mcgill.ca,
22, 99 -- lippmann-at-env.med.nyu.edu, james.lockey-at-uc.edu, toxpathmcc-at-aol.com,
22, 99 -- brooke.mossman-at-uvm.edu, gunter_oberdorster-at-urmc.rochester.edu,
22, 99 -- wwallace-at-cdc.gov, hgrzelka-at-lungusadc.org, j.wright-at-cellbio.duke.edu,
22, 99 -- Anne-Marie.Boullier-at-obs.ujf-grenoble.fr, info-at-eia-usa.org,
22, 99 -- bkynoch-at-eia-usa.org, krutt-at-kynoch.com, mwschrum-at-terracon.com,
22, 99 -- tom.laubenthal-at-atcassociates.com, bill-at-taiinfo.com, mebeard-at-rti.org,
22, 99 -- sniderjm-at-amrc-environmental.com, dnicodemus-at-amalab.com,
22, 99 -- mwschrum-at-terracon.com, mebeard-at-rti.org,
22, 99 -- doug-at-AsbestosDiseaseAwareness.org, amtic-at-epamail.epa.gov, emc-at-epa.gov,
22, 99 -- nelson.peg-at-epa.gov, r3public-at-epa.gov, r9.info-at-epa.gov, r8eisc-at-epa.gov,
22, 99 -- contact_NAREL-at-epa.gov, silvey.patricia-at-dol.gov, king.phillip-at-epa.gov,
22, 99 -- white.harold-at-epa.gov, mikel.dennisk-at-epa.gov, toney.mike-at-epa.gov,
22, 99 -- simons.tom-at-epa.gov, webster.daniel-at-epa.gov, jsikes-at-css.ua.edu,
22, 99 -- lch-at-adem.state.al.us, parker.moore-at-mail.state.ky.us,
22, 99 -- bickham_forshee-at-deq.state.ms.us, mary.giguere-at-ncmail.net,
22, 99 -- danny_dackson-at-deq.state.ms.us, linnert.ted-at-epa.gov,
22, 99 -- peronard.paul-at-epa.gov, r9.info-at-epa.gov, caldwell.carmen-at-epa.gov,
22, 99 -- caldwell.carmen-at-epa.gov, NVLAP-at-nist.gov, Microscopy-at-microscopy.com,
22, 99 -- info-at-chrysotile.com, nii-at-uraltc.ru, cba-at-duralit.net,
22, 99 -- eternity-at-dm.net.lb, info-at-gulf-eternit.com, ai-at-asbestos-institute.ca,
22, 99 -- imfi-at-avantel.net, jaa-at-blue.ocn.ne.jp, donac-at-hmc.vnn.vn,
22, 99 -- mudekunyew-at-arl.co.zw, Olle.selinus-at-sgu.se, Olle.selinus-at-gmail.com,
22, 99 -- Rbf-at-usgs.gov, centeno-at-afip.osd.mil, kimberley.chisholm-at-gmail.com,
22, 99 -- david.slaney-at-esr.cri.nz, berna-at-ige.unicamp.br, f.fordyce-at-bgs.ac.uk,
22, 99 -- dns-at-mail.dnttm.ro, p.weinstein-at-uq.edu.au, nmanay-at-fq.edu.uy,
22, 99 -- umran-dogan-at-uiowa.edu, cgomes-at-ua.pt, madeirarochas-at-netmadeira.pt,
22, 99 -- bkynoch-at-kynoch.com, rnolan-at-ierfinc.org, ierf-at-ierfinc.org,
22, 99 -- Hillary.carpenter-at-state.mn.us, wilson5-at-fas.harvard.edu,
22, 99 -- peter.bobrowsky-at-gems7.gov.bc.ca, jbunnell-at-usgs.gov,
22, 99 -- catherine.skinner-at-yale.edu, baughman-william-at-msha.gov,
22, 99 -- njeffery-at-health.nyc.gov, amj3-at-cdc.gov, fradkin.kenneth-at-epa.gov,
22, 99 -- john.hadley-at-owenscorning.com, dfg0-at-cdc.gov, fradkin.kenneth-at-epa.gov,
22, 99 -- janes-debra-at-msha.gov, lynch.katie-at-epa.gov, maddaloni.mark-at-epa.gov,
22, 99 -- catherine-at-asthmamoms.com, simons.tom-at-epa.gov, jes19-at-health.state.ny.us,
22, 99 -- ass1-at-cdc.gov, brian.toal-at-po.state.ct.us, jturim-at-sciences.com,
22, 99 -- rjw03-at-health.state.ny.us, katie.yagerman-at-mineralstech.com,
22, 99 -- rdz1-at-cdc.gov, davidb-at-iprolink.ch, pdumorti-at-ulb.ac.be, pic-at-fao.org,
22, 99 -- piC-at-unep.ch, jmillette-at-mvainc.com, jthomas-at-astm.org, jgrove-at-astm.org,
22, 99 -- kmorgan-at-astm.org, tbrooke-at-astm.org, dsmith-at-astm.org, bschultz-at-astm.org,
22, 99 -- plively-at-astm.org, smurphy-at-astm.org, tbrooke-at-astm.org,
22, 99 -- pgodorov-at-astm.org, amcklind-at-astm.org, dschultz-at-astm.org,
22, 99 -- ppicarie-at-astm.org, jadkins-at-astm.org, ppicarie-at-astm.org,
22, 99 -- lmorriss-at-astm.org, rmorgan-at-astm.org, gluciw-at-astm.org,
22, 99 -- dbradley-at-astm.org, smawn-at-astm.org, oberti-at-crystal.unipv.it,
22, 99 -- peter.ulmer-at-erdw.ethz.ch, christian.chopin-at-ens.fr, gervilla-at-ugr.es,
22, 99 -- herta.silvia.effenberger-at-univie.ac.at, amfransolet-at-ulg.ac.be,
22, 99 -- fhatert-at-ulg.ac.be, plefevre-at-ulg.ac.be, kniewald-at-mail.irb.hr
22, 99 -- Cc: silvana.capella-at-unito.it, alessandro.genovese-at-unito.it,
22, 99 -- donata.bellis-at-libero.it, donata.bellis-at-aslto2nord.it
22, 99 -- User-Agent: SquirrelMail/1.4.2
22, 99 -- MIME-Version: 1.0
22, 99 -- Content-Type: text/plain;charset=iso-8859-1
22, 99 -- Content-Transfer-Encoding: 8bit
22, 99 -- X-Confirm-Reading-To: elena.belluso-at-unito.it
22, 99 -- Disposition-Notification-To: elena.belluso-at-unito.it
22, 99 -- X-Priority: 3
22, 99 -- Importance: Normal
22, 99 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv
22, 99 -- X-Inrete-Amavisjob-Service-Runned: 6 (n2VB25A6020726)
22, 99 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (n2VB25A6020726)
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 31 Mar 2009 12:15:44 -0500
Subject: [Microscopy] Detached retina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I'm having problems with detached retina, not mine personally but samples I receive.

When I embed 3mm punch biopsies I have to bisect them. This causes the retina to detach from the back of the punch.

I've tried simple agar sandwiches--agar in a plate, retina on top and then a drop of agar on top of the punch--they separate when bisected.

They separate if you look at them wrong.

If you can give suggestions as to how to keep my retina attached it would be great.

I'd love to "see" how you keep them together.

Thanks,

Paula :-)

p.s. if you were alive in 1973 and want a radio music flashback google "Life on Mars Radio". It's playing great old rock & roll, some real deep cuts.

I have no relationship to this radio station, other than enjoying it before it goes off the internet when the series ends.


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
17, 25 -- From vapatpxs-at-yahoo.com Tue Mar 31 12:15:44 2009
17, 25 -- Received: from n27.bullet.mail.mud.yahoo.com (n27.bullet.mail.mud.yahoo.com [68.142.206.222])
17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2VHFiU8025537
17, 25 -- for {microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 12:15:44 -0500
17, 25 -- Received: from [209.191.108.97] by n27.bullet.mail.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000
17, 25 -- Received: from [68.142.201.243] by t4.bullet.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000
17, 25 -- Received: from [127.0.0.1] by omp404.mail.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000
17, 25 -- X-Yahoo-Newman-Property: ymail-3
17, 25 -- X-Yahoo-Newman-Id: 920524.6369.bm-at-omp404.mail.mud.yahoo.com
17, 25 -- Received: (qmail 14003 invoked by uid 60001); 31 Mar 2009 17:15:43 -0000
17, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238519743; bh=ZYnf3nzJUA5WLYz5ercnGo2fjhImBMHxZiy9uO2Na1I=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=4SLZoGkE7Pr4ENkBiEjl9GUtRaBUy9H9Zp/maLjg/vxOPG7s2aNYQfbxKltboeoeTrsyJMmDkHmqx73eVCzpbJoBCvcVhpKmcjCESSojhx8hfb8CBy/15kVLRu7z/U+Kctui5/uAADDfA3oa3F1Jkql7pgV7yglZ8fqFoKEhmis=
17, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
17, 25 -- s=s1024; d=yahoo.com;
17, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
17, 25 -- b=wItH0Kco7yQeTtjkf15vvuM+oMdTQjsD2l4MxkiZoZMfTGxWsMMlK6AEFJLTBiAbsH4fBUnrbr0ukk2GdoTX4SfKr1ZQTS3M2bxlQ3HbvDELsyCdvXdFuWLZZabBthmCS0yZh0OT0WjqmLU2LmjqFwPI8tRtYVrGiydLAlTvP+g=;
17, 25 -- Message-ID: {85710.1316.qm-at-web46110.mail.sp1.yahoo.com}
17, 25 -- X-YMail-OSG: 7lnM4OMVM1n_FVEYME2awnnc2WQia8Kex6dF84Ik.Ldx2XNKvFcRh6P.i3Tx.7mVTqNQ7loyPb3YHTzEcscJ1FBiIrPEgGZkqR8dRJMu8cPJAaCsdZ_UlHUCsZQT3o6vA81NRFHBYXo.92YYsEzKDoFKMNN53wxbpy2d1R14Tu9PH7MWqU1SKIDXpcMT1t4ese0Ck4opRUI4g2Y1cG.GkVydondIRzbkiNDg2KeW19mzVof765HO9ZuqAf8UyoIjag_8LY5MHTZ53d65OiQ-
17, 25 -- Received: from [132.239.85.200] by web46110.mail.sp1.yahoo.com via HTTP; Tue, 31 Mar 2009 10:15:43 PDT
17, 25 -- X-Mailer: YahooMailClassic/5.1.20 YahooMailWebService/0.7.289.1
17, 25 -- Date: Tue, 31 Mar 2009 10:15:43 -0700 (PDT)
17, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
17, 25 -- Subject: Detached retina
17, 25 -- To: MSA BB {Microscopy-at-microscopy.com}
17, 25 -- MIME-Version: 1.0
17, 25 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Tue, 31 Mar 2009 13:10:15 -0500
Subject: [Microscopy] Re: Detached retina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paula-
I have to admit that I liked the BBC version of LIfe On Mars better
(even if scenes for the first episode for the ABC series were filmed
in the park near my house in Queens - not Central Park as portrayed).

For your retinas...are you sure they are not already detached by the
biopsy procedure? I've dealt with many rat eyes, so I know what you
are going through. They always split at the RPE/ORS interface.
If you are fairly confident that they are intact when you get them,
leave them whole through the processing....just drag it out terribly
(30 minute dehydrations, many-stepped infiltration w/o accelerater,
drawn out over 2-3 days before going into final resin) and then, when
they are fully infiltrated, bisect them by cutting from the neural
retina down through the sclera. You may still get detachments along
the edge, but the middle should stay put.



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
8, 34 -- From lcgould-at-med.cornell.edu Tue Mar 31 13:10:15 2009
8, 34 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
8, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VIAFke018189
8, 34 -- for {microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 13:10:15 -0500
8, 34 -- MIME-version: 1.0
8, 34 -- Content-transfer-encoding: 7BIT
8, 34 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 34 -- Received: from mpx5.med.cornell.edu ([140.251.11.120])
8, 34 -- by mail-gw2.med.cornell.edu
8, 34 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
8, 34 -- with ESMTP id {0KHD00FCYUH12O00-at-mail-gw2.med.cornell.edu} for
8, 34 -- microscopy-at-microscopy.com; Tue, 31 Mar 2009 14:10:13 -0400 (EDT)
8, 34 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
8, 34 -- by mpx5.med.cornell.edu
8, 34 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
8, 34 -- with ESMTPA id {0KHD00046UH0IW60-at-mpx5.med.cornell.edu} for
8, 34 -- microscopy-at-microscopy.com; Tue, 31 Mar 2009 14:10:13 -0400 (EDT)
8, 34 -- X-PMX-Version: 5.5.2.365749, Antispam-Engine: 2.6.1.350677,
8, 34 -- Antispam-Data: 2009.3.31.175829
8, 34 -- X-Perlmx-Spam: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05,
8, 34 -- BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0,
8, 34 -- __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0,
8, 34 -- __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0,
8, 34 -- __HAS_MSGID 0, __KNOWN_PHONE_RU_812 0, __MEDS_PLAIN 0,
8, 34 -- __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0,
8, 34 -- __SANE_MSGID 0, __STOCK_PHRASE_24 0'
8, 34 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
8, 34 -- Message-id: {p0623091fc5f807826b3d-at-[140.251.48.23]}
8, 34 -- In-reply-to: {200903311727.n2VHRUEN006929-at-ns.microscopy.com}
8, 34 -- References: {200903311727.n2VHRUEN006929-at-ns.microscopy.com}
8, 34 -- Date: Tue, 31 Mar 2009 14:10:06 -0400
8, 34 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
8, 34 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
8, 34 -- Subject: Re: [Microscopy] Detached retina
==============================End of - Headers==============================




From: fundatel-at-gmail.com
Date: Tue, 31 Mar 2009 15:55:08 -0500
Subject: [Microscopy] Looking for TEM and SEM Donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listmembers....
We are looking for a SEM and TEM to receive donations on behalf of our
partners, the Del Plata Adventist University .
Both instruments must be in working condition.
All costs of packing and shipping will be paid by us

Please, contact us via email to info-at-fundatel.org.ar

Best Regards

--
Fundatel
Fundación de Telemedicina
Victoria 144
Parana
Entre Rios
Argentina


==============================Original Headers==============================
4, 32 -- From fundatel-at-gmail.com Tue Mar 31 15:55:08 2009
4, 32 -- Received: from yw-out-1718.google.com (yw-out-1718.google.com [74.125.46.153])
4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VKt8Rh007508
4, 32 -- for {microscopy-at-Microscopy.Com} ; Tue, 31 Mar 2009 15:55:08 -0500
4, 32 -- Received: by yw-out-1718.google.com with SMTP id 6so2046758ywa.0
4, 32 -- for {microscopy-at-Microscopy.Com} ; Tue, 31 Mar 2009 13:55:07 -0700 (PDT)
4, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 32 -- d=gmail.com; s=gamma;
4, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
4, 32 -- :from:to:content-type:content-transfer-encoding;
4, 32 -- bh=5ukdSKfKVOzPIrqIoEcSbrii9CYjPbhvfq4E8QVPl08=;
4, 32 -- b=IxDkhZha6LotVhyfJWh0SYvSJIT12QZMTpbDk9EzUaS+jtGeVmn680vn1IjbuFGgWx
4, 32 -- E3AjQVH8bL+abcNBlvWRW2YwJfCbKhBCUNu9u9S7C4s6KNutKoag/UUiLQYBP7mtBod4
4, 32 -- PsNSbNlCqwsHVqmICJhvBJOR+Y+lthSfipM8U=
4, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 32 -- d=gmail.com; s=gamma;
4, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
4, 32 -- :content-transfer-encoding;
4, 32 -- b=YC3LswZQoDmYguCr0x7NmxGcbp3tlQ+l2cKHg1zg6hQhF9H5RfXRCI/HeyxOAw6Nqw
4, 32 -- fWCPHAVNuHO+3Jl61r5hJSrjNouFV4epLeAVAKqb17piDiozeBxpxPiM3y4Kl32O/mx5
4, 32 -- QS7cauCbBUs870zk8mGpVjcuamirvrnC/zBbo=
4, 32 -- MIME-Version: 1.0
4, 32 -- Received: by 10.151.82.10 with SMTP id j10mr12955929ybl.3.1238532907719; Tue,
4, 32 -- 31 Mar 2009 13:55:07 -0700 (PDT)
4, 32 -- Date: Tue, 31 Mar 2009 17:55:07 -0300
4, 32 -- Message-ID: {715613900903311355y79d650cal42d30c524eaf1ccb-at-mail.gmail.com}
4, 32 -- Subject: Looking for TEM and SEM Donation
4, 32 -- From: Fundatel {fundatel-at-gmail.com}
4, 32 -- To: microscopy-at-Microscopy.Com
4, 32 -- Content-Type: text/plain; charset=ISO-8859-1
4, 32 -- Content-Transfer-Encoding: 8bit
4, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2VKt8Rh007508
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Tue, 31 Mar 2009 16:13:28 -0500
Subject: [Microscopy] OM - resolution rule of thumb?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would probably look into one of John Russ's books on image processing and measurement. I don't have an exact citation. Working in the field, I would say this issue is practically intuitively obvious when explained.

You should understand that measurements will always be plus or minus one (or more) pixels. I would be comfortable with 2-micron pixels to detect 6-micron features, but the exact diameter or area will be less precise. I like to have more than 10 pixels across the diameter of a moderately sized particle. I could live with fewer pixels across the diameter on the smaller end of the distribution.

However, there is also often a significant effect due to the threshold setting. Fuzziness on the edge leads to larger particles if the threshold is set closer to the background brightness. A setting midway between background and feature brightness is preferred to evenly split the uncertainty.

One of the corollaries to that principle is that small particles will suffer underestimation as their maximum brightness will often be less than that of larger particles. For instance, if the fuzziness of the edge extends over 3 pixels on each side, and the particle is only 5 pixels wide, then the center pixel is going to be less than full brightness. The particle will appear smaller than its true size. More magnification (not just more pixels) may be necessary to achieve full brightness and eliminate the effect. This is fairly easy to demonstrate on an SEM with a brightness waveform display. I suppose you should also be able to visualize this effect by plotting brightness along a linear traverse across various sized particles.

IMHO, it is better to understand many of these principles for yourself and be able to explain them and your choices than to blithely state that a procedure was done in accordance with some numbered procedure. Standard procedures do not guarantee accuracy (as I can attest from some ASTM coal analysis procedures). However, they do guarantee that everyone will be wrong in the same way and presumably to the same extent.

Warren S.

X-from: Jacqueline.Ayotte-at-ticona.com [mailto:Jacqueline.Ayotte-at-ticona.com]
Sent: Mon 3/30/2009 8:39 AM
To: wesaia-at-iastate.edu


Hello All,

Is there a documented rule of thumb regarding minimum resolution per
pixel when measuring particles of a certain size range in an image? For
example, if one were interested in measuring particles down to 6 microns
would one set magnification to yield 3 microns per pixel or 2 microns
per pixel in the acquired image? (or less?)

Again, looking for documentation on this. I need to demonstrate proper
resolution to peers in Europe and it is not enough that I just "say so"
without any supportive data.

Thank you for any help or pointers.

Regards,
Jackie

Jacqueline Ayotte
Microscopist - Advanced Materials Characterization
        Ticona
        8040 Dixie Highway
        Florence KY  41042
        859-372-3139
        fax 859-372-3184
        jacqueline.ayotte-at-ticona.com
The information contained in this e-mail, and any attachments thereto,
is confidential and is intended only for use by the individual(s) and/or
entity named above. If you are not the intended recipient of this
e-mail, you are hereby notified that any dissemination, distribution or
copying of this communication or any disclosure of the contents of this
communication to others is strictly prohibited. If you have received
this communication in error, please notify the sender immediately by
replying to this e-mail. Please then delete the original including all
attachments and any copy of any e-mail and printout thereof.

==============================Original Headers==============================
11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009
11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com [64.18.2.215])
==============================End of - Headers==============================


==============================Original Headers==============================
16, 35 -- From wesaia-at-iastate.edu Tue Mar 31 16:13:28 2009
16, 35 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14])
16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VLDRLf021994
16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:27 -0500
16, 35 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
16, 35 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n2VLDQRW016922
16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:26 -0500
16, 35 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
16, 35 -- id 7304_c6fce34a_1e38_11de_8969_001372578af6;
16, 35 -- Tue, 31 Mar 2009 16:13:27 -0500
16, 35 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
16, 35 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n2VLD4uv003782
16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:19 -0500
16, 35 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
16, 35 -- Tue, 31 Mar 2009 16:12:58 -0500
16, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 35 -- Content-class: urn:content-classes:message
16, 35 -- MIME-Version: 1.0
16, 35 -- Content-Type: text/plain;
16, 35 -- charset="iso-8859-1"
16, 35 -- Subject: RE: [Microscopy] OM - resolution rule of thumb?
16, 35 -- Date: Tue, 31 Mar 2009 16:10:15 -0500
16, 35 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703C246AE-at-maire.eng.iastate.edu}
16, 35 -- X-MS-Has-Attach:
16, 35 -- X-MS-TNEF-Correlator:
16, 35 -- Thread-Topic: [Microscopy] OM - resolution rule of thumb?
16, 35 -- thread-index: AcmxPPPodyQy53QlRXSh93lkgKzm1gAAGnjy
16, 35 -- References: {200903301339.n2UDdPuk025070-at-ns.microscopy.com}
16, 35 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
16, 35 -- To: {Jacqueline.Ayotte-at-ticona.com} , {Microscopy-at-microscopy.com}
16, 35 -- X-OriginalArrivalTime: 31 Mar 2009 21:12:58.0371 (UTC) FILETIME=[77798530:01C9B245]
16, 35 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.31.205818
16, 35 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0'
16, 35 -- Content-Transfer-Encoding: 8bit
16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2VLDRLf021994
==============================End of - Headers==============================




From: john.brealey-at-imvs.sa.gov.au
Date: Tue, 31 Mar 2009 17:57:49 -0500
Subject: [Microscopy] Comments on Glycogen - Thankyou!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Just saying thankyou to everyone who replied to my query re staining for
glycogen in resin-embedded tissue (see below).
Personally I agree with Geoff McAuliffe and others who suggested morphology
should suffice. He commented that not all hepatocytes contain the same
amount of glycogen which I found interesting and reminded me of the
uncertainty related to sampling issues in TEM studies. Our researcher has
demonstrated a rise in intracellular glycogen in several of his tests so I
believe he is genuinely onto something and is not being duped by the
vagaries of TEM sampling issues.
Thanks to Terry Robertson, Stephane and others who provided protocols for
various staining techniques.
Thanks to Wolfgang Muss and Donald Gantz for their comprehensive replies and
their comments regarding pH issues and the leaching of glycogen when using
en-bloc staining with uranyl acetate.

Regards,

John Brealey

Hi,

We have a researcher who is studying the effects of a particular drug on rat
liver, heart and spinal cord.
Tissue for EM was processed with osmium tetroxide, en-bloc stained with
uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy
resin. Thin sections were stained with lead citrate.
By EM we found increased amounts of glycogen in one of the liver samples.
The researcher asked "How do you know it's glycogen".
I said "It just is".
He is worried that his supervisor won't accept that it's glycogen just
because we said so. He wants to prove it.

So my question is...

Is there a stain for epoxy resin sections that will stain specifically for
glycogen?
Will periodic acid Schiff (PAS) work on resin-embedded sections?

Regards,

John Brealey


Supervisor - Electron Microscope Unit



E john.brealey-at-imvs.sa.gov.au

T 8222 6612

F 8222 6425

www.sapathology.sa.gov.au



SA Pathology (Queen Elizabeth Hospital)



Electron Microscope Unit, Surgical Pathology

SA Pathology

Queen Elizabeth Hospital

Woodville, 5011

AUSTRALIA


==============================Original Headers==============================
27, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 31 17:57:49 2009
27, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14])
27, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VMvmvM007557
27, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 17:57:49 -0500
27, 27 -- X-IronPort-AV: E=Sophos;i="4.39,304,1235914200";
27, 27 -- d="scan'208";a="16432857"
27, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85])
27, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 01 Apr 2009 09:27:46 +1030
27, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by
27, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id
27, 27 -- 8.1.263.0; Wed, 1 Apr 2009 09:26:43 +1030
27, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by
27, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id BE8BA34047 for
27, 27 -- {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 09:27:46 +1030 (CST)
27, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au}
27, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au}
27, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
27, 27 -- Subject: Comments on Glycogen - Thankyou!
27, 27 -- Date: Wed, 1 Apr 2009 09:27:46 +1030
27, 27 -- Organization: IMVS
27, 27 -- Message-ID: {000901c9b254$1b743f50$7d8a140a-at-41347i}
27, 27 -- MIME-Version: 1.0
27, 27 -- Content-Type: text/plain; charset="us-ascii"
27, 27 -- Content-Transfer-Encoding: 7bit
27, 27 -- X-Mailer: Microsoft Office Outlook 11
27, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
27, 27 -- Thread-Index: AcmyVBtOs6JuFEyqTsKzJfhQAIwhJg==
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 1 Apr 2009 02:55:51 -0500
Subject: [Microscopy] gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Our gold target has ended its useful life and there I am with 4,5 grams of pure gold which does not belong to me.
I would feel bad to take it at home, and even worse to dump it with the heavy metals ;-)
Now I cannot say that targets are very complex pieces of technology, so I suppose that the expensive price of gold targets is mainly explained by the price of the metal itself, meaning that recycling would be a very precious way to acquire a new target for fewer cents (or are they dollars?). Our current provider told us they don't recycle.
Is there someone here who does? (in Europe)

Regards,

Stephane





==============================Original Headers==============================
8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009
8, 20 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n317tpEg015074
8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 02:55:51 -0500
8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr 2009 07:55:50 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238572550; bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q68J41JkbaSK16Nptj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA42R8P0uWCyyZ3pZhK7GYh0mIbSRdSXafRdMYv9BT5ANCkHHt8=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2WqDiil4PyXzkekD75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2vBR5Ldtb8EMybqN0mohvTOigNk+dBpkfh7OvirT61cpS4a2cc=;
8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com}
8, 20 -- X-YMail-OSG: 9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJIOUyJowZuXMdA0EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3_psq1BWxEx4Hqooq50pn9AruHygSmey5r8esiqQXDLJKJzPWObFyx7eundx5MRTC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGSTcwEwbHCahplAS9engGNZvVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23xtoM7g--
8, 20 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Wed, 01 Apr 2009 00:55:50 PDT
8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT)
8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
8, 20 -- Subject: gold target: second life?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Wed, 1 Apr 2009 03:25:42 -0500
Subject: [Microscopy] gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane:

I worked for a precious metal refiner for a short while in Los Angeles. Most
refiners will certainly process and refine your gold scrap. You can easily
find a local one in EC by just Google. It may not be worth the minimum
processing fee for 4g of gold. Do you have other gold or Pt scrap to
consolidate?

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.



Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Wednesday, April 01, 2009 1:06 AM
To: dkloos-at-parallaxray.com


Dear all,

Our gold target has ended its useful life and there I am with 4,5 grams of
pure gold which does not belong to me.
I would feel bad to take it at home, and even worse to dump it with the
heavy metals ;-)
Now I cannot say that targets are very complex pieces of technology, so I
suppose that the expensive price of gold targets is mainly explained by the
price of the metal itself, meaning that recycling would be a very precious
way to acquire a new target for fewer cents (or are they dollars?). Our
current provider told us they don't recycle.
Is there someone here who does? (in Europe)

Regards,

Stephane





==============================Original Headers==============================
8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009
8, 20 -- Received: from web110814.mail.gq1.yahoo.com
(web110814.mail.gq1.yahoo.com [67.195.13.237])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n317tpEg015074
8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 02:55:51
-0500
8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr 2009 07:55:50
-0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1238572550; bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q68J41JkbaSK16Np
tj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA42R8P0uWCyyZ3pZhK7GYh0mIbSRd
SXafRdMYv9BT5ANCkHHt8=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
8, 20 --
b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2WqDiil4PyXzkekD
75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2vBR5Ldtb8EMybqN0mohvTOigNk+d
Bpkfh7OvirT61cpS4a2cc=;
8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com}
8, 20 -- X-YMail-OSG:
9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJIOUyJowZuXMdA0
EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3_psq1BWxEx4Hqooq50pn9AruHygS
mey5r8esiqQXDLJKJzPWObFyx7eundx5MRTC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGST
cwEwbHCahplAS9engGNZvVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23
xtoM7g--
8, 20 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via
HTTP; Wed, 01 Apr 2009 00:55:50 PDT
8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT)
8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
8, 20 -- Subject: gold target: second life?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================


==============================Original Headers==============================
18, 30 -- From dkloos-at-parallaxray.com Wed Apr 1 03:25:40 2009
18, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n318Pd4G002870
18, 30 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 03:25:39 -0500
18, 30 -- Received: from [63.80.79.36] (helo=donl)
18, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
18, 30 -- (envelope-from {dkloos-at-parallaxray.com} )
18, 30 -- id 1LovlU-00044l-L3; Wed, 01 Apr 2009 02:25:40 -0600
18, 30 -- Reply-To: {dkloos-at-parallaxray.com}
18, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
18, 30 -- To: {nizets2-at-yahoo.com}
18, 30 -- Cc: {microscopy-at-microscopy.com}
18, 30 -- References: {200904010806.n3186AkV030168-at-ns.microscopy.com}
18, 30 -- Subject: RE: [Microscopy] gold target: second life?
18, 30 -- Date: Wed, 1 Apr 2009 01:25:37 -0700
18, 30 -- Organization: Parallax Research
18, 30 -- Message-ID: {BDC3DADD7D754577851EA74312641181-at-donl}
18, 30 -- MIME-Version: 1.0
18, 30 -- Content-Type: text/plain;
18, 30 -- charset="us-ascii"
18, 30 -- Content-Transfer-Encoding: 7bit
18, 30 -- X-Mailer: Microsoft Office Outlook 11
18, 30 -- Thread-Index: AcmyoLiyTUfh2cOUT7CeZtWwoiyBEAAAmVVw
18, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
18, 30 -- In-Reply-To: {200904010806.n3186AkV030168-at-ns.microscopy.com}
18, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
18, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
18, 30 -- X-AntiAbuse: Original Domain - microscopy.com
18, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
18, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Wed, 1 Apr 2009 03:27:22 -0500
Subject: [Microscopy] Re: gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,

In Europe (i.e. at least Austria&Germany) two } recyclers { for precious (heavy) metals I know of:

E.g. A) see http://www.degussa.com/degussa/en/ (English) ==} as of 12th September 2007 DEGUSSA is part of ==} EVONIK
==} www.evonik.com
http://www.degussa.com/degussa/de/ (German)

==} find "SEARCH" ==} "metals"
==} result = Base Metal Refining Services:
Contact:
Evonik Degussa GmbH
Business Line Catalysts
Rodenbacher Chaussee 4
63457 Hanau
Germany
T: +49-6181-59-8722
F: +49-6181-59-2699
degussa_catalysts-at-degussa.com


E.g. B) AUSTRIA: OEGUSSA http://www.oegussa.at/ ==} click in menuebar Recycling:
==} http://www.oegussa.at/neu/recycling/index.htm

Gold-, silber-, platin- oder palladiumhältiges Scheidgut
Ansprechpartner: Erich Siegler
01-866 46 DW 4153
01-866 46 DW 4154
Oder in Ihrer Ögussa-Filiale:
http://www.oegussa.at/neu/standorte/filialen.htm

==} Wien, Gumpendorfer Straße 85, A 1060 Wien
Tel. +43 1 599 61 - 225
Fax +43 1 599 61 - 310

e-mail: office.gumpendorf-at-oegussa.at
Öffnungszeiten: Montag - Donnerstag: 7.30 - 16.30 Uhr
Freitag: 7.30 - 12.45 Uhr

You won't get easy rid of your gold target perhaps, despite the global gold price at the moment is quite good...
Important to know (IMHO) is the quality / pure Au - metal content of the target left.

Another possibility would be : offer to jeweler, watch maker(s) on a "private honorary" basis...


Best wishes and good luck,
Mit besten Gruessen,

Wolfgang Muss
Salzburg, Austria







} -----Ursprüngliche Nachricht-----
} Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
} Gesendet: Mittwoch, 01. April 2009 10:01
} An: Muß Wolfgang
} Betreff: [Microscopy] gold target: second life?
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
} Dear all,
}
} Our gold target has ended its useful life and there I am with
} 4,5 grams of pure gold which does not belong to me.
} I would feel bad to take it at home, and even worse to dump
} it with the heavy metals ;-)
}
} Now I cannot say that targets are very complex pieces of
} technology, so I suppose that the expensive price of gold
} targets is mainly explained by the price of the metal itself,
} meaning that recycling would be a very precious way to
} acquire a new target for fewer cents (or are they dollars?).
} Our current provider told us they don't recycle.
} Is there someone here who does? (in Europe)
}
} Regards,
}
} Stephane
}
}
}
}
}
} ==============================Original
} Headers==============================
} 8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009
} 8, 20 -- Received: from web110814.mail.gq1.yahoo.com
} (web110814.mail.gq1.yahoo.com [67.195.13.237])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id n317tpEg015074
} 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr
} 2009 02:55:51 -0500
} 8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr
} 2009 07:55:50 -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256;
} c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238572550;
} bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=;
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
} o:MIME-Version:Content-Type;
} b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q6
} 8J41JkbaSK16Nptj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA
} 42R8P0uWCyyZ3pZhK7GYh0mIbSRdSXafRdMYv9BT5ANCkHHt8=
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 --
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
} o:MIME-Version:Content-Type;
} 8, 20 --
} b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2W
} qDiil4PyXzkekD75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2
} vBR5Ldtb8EMybqN0mohvTOigNk+dBpkfh7OvirT61cpS4a2cc=;
} 8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com}
} 8, 20 -- X-YMail-OSG:
} 9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJ
} IOUyJowZuXMdA0EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3
} _psq1BWxEx4Hqooq50pn9AruHygSmey5r8esiqQXDLJKJzPWObFyx7eundx5MR
} TC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGSTcwEwbHCahplAS9engGNZ
} vVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23xtoM7g--
} 8, 20 -- Received: from [80.122.101.100] by
} web110814.mail.gq1.yahoo.com via HTTP; Wed, 01 Apr 2009 00:55:50 PDT
} 8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
} 8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT)
} 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 8, 20 -- Subject: gold target: second life?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
19, 36 -- From W.Muss-at-salk.at Wed Apr 1 03:27:21 2009
19, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
19, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n318RLaa006637
19, 36 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 03:27:21 -0500
19, 36 -- Received: from localhost (localhost [127.0.0.1])
19, 36 -- by hermes.salk.at (Postfix) with ESMTP id 3398CC385D;
19, 36 -- Wed, 1 Apr 2009 10:27:20 +0200 (CEST)
19, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
19, 36 -- Received: from hermes.salk.at ([127.0.0.1])
19, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
19, 36 -- with ESMTP id e3jYBbHFsVJ9; Wed, 1 Apr 2009 10:27:19 +0200 (CEST)
19, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
19, 36 -- by hermes.salk.at (Postfix) with ESMTP id BF2F4C3832;
19, 36 -- Wed, 1 Apr 2009 10:27:19 +0200 (CEST)
19, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
19, 36 -- Wed, 1 Apr 2009 10:27:20 +0200
19, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 36 -- Content-class: urn:content-classes:message
19, 36 -- MIME-Version: 1.0
19, 36 -- Content-Type: text/plain;
19, 36 -- charset="iso-8859-1"
19, 36 -- Subject: [Microscopy] Re: gold target: second life?
19, 36 -- Date: Wed, 1 Apr 2009 10:27:19 +0200
19, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0938-at-N1RZ116.lksdom21.lks.local}
19, 36 -- In-Reply-To: {200904010800.n3180ZGK021610-at-ns.microscopy.com}
19, 36 -- X-MS-Has-Attach:
19, 36 -- X-MS-TNEF-Correlator:
19, 36 -- Thread-Topic: [Microscopy] Re: gold target: second life?
19, 36 -- Thread-Index: Acmyn/V7F/69LWGpRUWkFW98eQpQpQAAY4Lw
19, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
19, 36 -- To: {nizets2-at-yahoo.com}
19, 36 -- Cc: {microscopy-at-microscopy.com}
19, 36 -- X-OriginalArrivalTime: 01 Apr 2009 08:27:20.0021 (UTC) FILETIME=[AC7F5850:01C9B2A3]
19, 36 -- X-Scanned-By: SALK-Content-Filter
19, 36 -- Content-Transfer-Encoding: 8bit
19, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n318RLaa006637
==============================End of - Headers==============================




From: michelle.gignac-at-duke.edu
Date: Wed, 1 Apr 2009 08:12:50 -0500
Subject: [Microscopy] viaWWW: Ultracut Manual Clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both michelle.gignac-at-duke.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: michelle.gignac-at-duke.edu
Name: Michelle Gignac

Organization: Duke University

Title-Subject: [Filtered] Ultracut Manual Clarification

Question: Thanks to all that responded to the first message.

The microtome is an AO/Reichert Ultracut, Type 701701. I could not
find a model designation on it. It looks like an all grey Ultracut
E. The control unit is different. It does not have the cutting
thickness controls on it. I was fortunate to receive a copy of this
manual from another listserve member (Thanks again Dale!). If anyone
has a pdf version, I would appreciate a copy.

Thanks,
Michelle

Login Host: 152.3.194.20
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Apr 1 08:12:49 2009
8, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n31DCjxL013498
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 08:12:48 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240806c5f918baad83-at-[206.69.208.22]}
8, 11 -- Date: Wed, 1 Apr 2009 08:12:42 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: michelle.gignac-at-duke.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Ultracut Manual Clarification
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wzhe-at-laurentian.ca
Date: Wed, 1 Apr 2009 16:09:01 -0500
Subject: [Microscopy] viaWWW: Imaging plates for TEM Photo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wzhe-at-laurentian.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wzhe-at-laurentian.ca
Name: William

Organization: Luarentian Univ

Title-Subject: [Filtered] Imaging plates for TEM Photo

Question: Dear there,

We want to use Fuji imaging plates in TEM photo work and read some
recommend on product FDL-5000 plate. If someone could comment on this
imaging plate

1. What performance looks like in TEM photos?
2. Any alternate we can try, because this plate is quite expensive to
start with, ~US$4000 for a 16 pieces/pack ?

Thanks very much in advance,

William

Login Host: 142.51.53.72
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Apr 1 16:09:00 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n31L90gf016265
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 16:09:00 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c5f9885ad79a-at-[206.69.208.22]}
10, 11 -- Date: Wed, 1 Apr 2009 16:08:59 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: wzhe-at-laurentian.ca (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Imaging plates for TEM Photo
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Wed, 1 Apr 2009 17:47:58 -0500
Subject: [Microscopy] Zeiss Photomik III

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

Boy did I step in it this time!

I was in the process of changing the HBO 100 in my Zeiss Photomik III when I discovered a broken condenser lens. After waiting for the lens to arrive (made by bridge trolls in Austria) and having my lab shut down for 6 weeks for A/C repairs, I've forgotten how to put the lamp housing and bulb assembly back together.

My original Zeiss instructions show a grounding pin and I have a grounding wire. I think it has to connect to the diffuser thingy that the top of the bulb goes into but I can't figure out where. It could attach to the bottom but doesn't look like it by the way the grounding wire is bent.

If anyone can help me it would be great.

That's what I get for not taking notes. It made sense at the time but my old lady brain forgot the original configuration after almost 3 months after taking it apart.

Please don't nag me for not putting it together again once I discovered the broken lens condenser. I know that now.

It is a beautiful microscope with great optics and it looks like Giger designed it. I could look at it all day but I really want to look through it for lovely fluorescence and light images.

Thanks in advance for any help/suggestions you can send my way.

Paula :-}

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
15, 25 -- From vapatpxs-at-yahoo.com Wed Apr 1 17:47:58 2009
15, 25 -- Received: from n59.bullet.mail.sp1.yahoo.com (n59.bullet.mail.sp1.yahoo.com [98.136.44.43])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n31MltBj005912
15, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 17:47:57 -0500
15, 25 -- Received: from [69.147.84.144] by n59.bullet.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000
15, 25 -- Received: from [69.147.65.152] by t6.bullet.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000
15, 25 -- Received: from [127.0.0.1] by omp400.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000
15, 25 -- X-Yahoo-Newman-Property: ymail-3
15, 25 -- X-Yahoo-Newman-Id: 381515.93336.bm-at-omp400.mail.sp1.yahoo.com
15, 25 -- Received: (qmail 71772 invoked by uid 60001); 1 Apr 2009 22:47:54 -0000
15, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238626074; bh=yi/ySkCnQdEBuV//1r5VM+Q8HcQtYGmz2Kz9f4A76hg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=3iqgALkf31j/1pZ/jOxdFZ5daqV+JrcCfNa7Ryo2AVH0LmB7DrHwnrWWA7Vfnpe7u21FpVAAfC55C1SShdJnBnhiIHVdgWhu3RPjpnFz7PuLZsvqxXE7trfkzEr9zzWRY4iVJs4t85+NwJc1Cw+0p2S65nfEG9LOoDS0v9iSTec=
15, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
15, 25 -- s=s1024; d=yahoo.com;
15, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
15, 25 -- b=cqymkDPdotRlVwJLCarm1vrnlrli9SkmwpLXEZUWUNNVNIbbqmKY+0jxb/TMcZoeCI3MnFIBWfYc3gHfAYYA8KoSSRexKgjcgjGiseN9xtyXq/Loj+I5RscFFXhwJ5hSemNMf3ykG2+fgQWJY1r1YnpPhcFZQH/M1s8zqveRI5E=;
15, 25 -- Message-ID: {250358.71222.qm-at-web46108.mail.sp1.yahoo.com}
15, 25 -- X-YMail-OSG: tf2U8tMVM1n2QFDEC00xqk4K7hlVauLxV0bnbxuplB_tNFAUHp6LEgCoiJa_Xy3jLQHs19V8hQz49PBsswW8KnFJH94RZsqxOmI.ZVHwFdCz7epwH4JTxmMYYa3xux4f9ZsfQ7MfeTTZfj2l8CYIC8R2Lv.9ufyBT_wOaBqD5zCNTLDj1UGcF_p8E5GY.whPAfRo.DcCq1_8UJYvZeWzkFFN._UERgCxA5UP7Z2OyPgjw73jme.6Hx3qE_CbKI45h7P8g8VjjEAIAN.Kw48-
15, 25 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Wed, 01 Apr 2009 15:47:54 PDT
15, 25 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
15, 25 -- Date: Wed, 1 Apr 2009 15:47:54 -0700 (PDT)
15, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
15, 25 -- Subject: Zeiss Photomik III
15, 25 -- To: MSA BB {Microscopy-at-microscopy.com}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: yvan_lindekens-at-yahoo.com
Date: Thu, 2 Apr 2009 04:11:52 -0500
Subject: [Microscopy] LM: Fisher Histomatic 166A tissue processor, looking for manual.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I'm looking for an instruction manual for a Fisher Histomatic 166A tissue processor. A PDF or a xerox copy would be great. Off course I'm willing to pay the costs!

These manuals seem very hard to find and I begin to think that Fisher didn't even bother to make one...

I asked the Fisher representatives in Belgium. Their answer: "O, did we manufacture those?". Well... At least they answered.

Thanks in advance,

Yvan Lindekens.




==============================Original Headers==============================
9, 20 -- From yvan_lindekens-at-yahoo.com Thu Apr 2 04:11:52 2009
9, 20 -- Received: from web110206.mail.gq1.yahoo.com (web110206.mail.gq1.yahoo.com [67.195.8.182])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n329BpTT010864
9, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 04:11:51 -0500
9, 20 -- Received: (qmail 82225 invoked by uid 60001); 2 Apr 2009 09:11:51 -0000
9, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238663510; bh=TACZc3zwLd3v/Zdz2k1+DY0cKKw1TRm6IPlM0GBxaTY=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=dCvpCjiYunbW+1Li9BduCurrHMo2NaQdi8/M+IpOyj2sYir7a77TzXHtjk8f8yJn7OjJ4ffrAioQmpUB6k5j21Pm7d4KrrhzuQ0munrQB7Q8EX7HWTesz2knO+aMr2Ug1m/GptardQasndivm5282TzSKnca9SZH6mzXXv48UbA=
9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
9, 20 -- s=s1024; d=yahoo.com;
9, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
9, 20 -- b=HBzwEkJZK5txW9i1PNkuLiZuiQm476CvIoC9RxMIAXLflHTwwvEj3osdAgKt4nAULBnlGbFRDnRxz5W4W23eMQ1Sa0SfNBBbGuOJ8Q8GOD8c1kF+qUd17NxcoYF+eRGqGlbvgKKlv7kVAjmNxLIhi5tOz6er6TUyT1Avx5uJUTc=;
9, 20 -- Message-ID: {944309.81905.qm-at-web110206.mail.gq1.yahoo.com}
9, 20 -- X-YMail-OSG: mJt0urgVM1nUumxmavV9v.IE_sqpobj5TIloCBqOwPNuPw19oG86F_ZRkZaS5mg9URyZlAjIB4Uh6fQ58UF.IeBTIxPzYSdABHgMRIV6zYGGasLAJmu0dn1ejuqHOL.OtGw.IYBl62ODc2OuexoPpDUUGAuV4Ptj.UKuA1lh.tv6tRB44GLpn5_IqT8nx_uQ2n7.A1Dw.cCk_z_ynQfttyx_ePOYnvDs5T71.IsWtLIWr9u5Vh8_OHTriKp6PXmpJLQWtvIeJiS.MhmcjG58kzc.sAFsV8IUo9QnLnLG69MkAjDTKw--
9, 20 -- Received: from [87.64.33.117] by web110206.mail.gq1.yahoo.com via HTTP; Thu, 02 Apr 2009 02:11:50 PDT
9, 20 -- X-Mailer: YahooMailWebService/0.7.289.1
9, 20 -- Date: Thu, 2 Apr 2009 02:11:50 -0700 (PDT)
9, 20 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com}
9, 20 -- Subject: LM: Fisher Histomatic 166A tissue processor, looking for manual.
9, 20 -- To: Microscopy-at-microscopy.com
9, 20 -- MIME-Version: 1.0
9, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Thu, 2 Apr 2009 05:22:20 -0500
Subject: [Microscopy] Re: gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bonjours Stéphane

I give my old sputter targets to a jeweler which remelts it and laminate
a new foil (0.2-0.3 mm thick). I cut then 2-3 disks (~50 mm diameter) in
the foil, and the falls return to the box until the next remelting. It
cost me a few tens of euro all 2-3 years.

Of coarse it's only jewelry 24 carats purity and not 12N ! But it's
enough for the few works which need gold coating. And it's the same gold
we use for UHV gaskets, which the jeweler makes whith the old flatten ones.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



nizets2-at-yahoo.com a écrit :
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Dear all,
}
} Our gold target has ended its useful life and there I am with 4,5 grams of pure gold which does not belong to me.
} I would feel bad to take it at home, and even worse to dump it with the heavy metals ;-)
} Now I cannot say that targets are very complex pieces of technology, so I suppose that the expensive price of gold targets is mainly explained by the price of the metal itself, meaning that recycling would be a very precious way to acquire a new target for fewer cents (or are they dollars?). Our current provider told us they don't recycle.
} Is there someone here who does? (in Europe)
}
} Regards,
}
} Stephane
}
}
}
}
}
} ==============================Original Headers==============================
} 8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009
} 8, 20 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n317tpEg015074
} 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 02:55:51 -0500
} 8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr 2009 07:55:50 -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238572550; bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q68J41JkbaSK16Nptj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA42R8P0uWCyyZ3pZhK7GYh0mIbSRdSXafRdMYv9BT5ANCkHHt8=
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
} 8, 20 -- b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2WqDiil4PyXzkekD75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2vBR5Ldtb8EMybqN0mohvTOigNk+dBpkfh7OvirT61cpS4a2cc=;
} 8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com}
} 8, 20 -- X-YMail-OSG: 9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJIOUyJowZuXMdA0EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3_psq1BWxEx4Hqooq50pn9AruHygSmey5r8esiqQXDLJKJzPWObFyx7eundx5MRTC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGSTcwEwbHCahplAS9engGNZvVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23xtoM7g--
} 8, 20 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Wed, 01 Apr 2009 00:55:50 PDT
} 8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
} 8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT)
} 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 8, 20 -- Subject: gold target: second life?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
8, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Apr 2 05:22:20 2009
8, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32AMJln027062
8, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 05:22:20 -0500
8, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
8, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n32AMIRn066497
8, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 12:22:18 +0200 (CEST)
8, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
8, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 32 -- (No client certificate requested)
8, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 37C9A3EC003
8, 32 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 12:20:33 +0200 (CEST)
8, 32 -- Message-ID: {49D491CE.6080202-at-ipcms.u-strasbg.fr}
8, 32 -- Date: Thu, 02 Apr 2009 12:22:06 +0200
8, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
8, 32 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
8, 32 -- MIME-Version: 1.0
8, 32 -- To: Microscopy-at-microscopy.com
8, 32 -- Subject: Re: [Microscopy] gold target: second life?
8, 32 -- References: {200904010802.n3182H26024078-at-ns.microscopy.com}
8, 32 -- In-Reply-To: {200904010802.n3182H26024078-at-ns.microscopy.com}
8, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-IPCMS-MailScanner: Found to be clean
8, 32 -- X-IPCMS-MailScanner-SpamScore: sss
8, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
8, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 02 Apr 2009 12:22:18 +0200 (CEST)
8, 32 -- X-Virus-Scanned: ClamAV 0.94.2/9198/Thu Apr 2 08:50:41 2009 on mr4.u-strasbg.fr
8, 32 -- X-Virus-Status: Clean
8, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
8, 32 -- autolearn=disabled version=3.2.5
8, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 2 Apr 2009 10:02:29 -0500
Subject: [Microscopy] gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you to all for your answers/comments!
We have only 1 target, it is too few for anybody to care about this amount alone.
But I was directed to a company in Germany called "BALTIC Präparation" which recycles the gold and deduces a part of its value from the new target:
I give this information in case anybody else need it too.

Regards,

Stephane





==============================Original Headers==============================
7, 24 -- From nizets2-at-yahoo.com Thu Apr 2 10:02:29 2009
7, 24 -- Received: from web110813.mail.gq1.yahoo.com (web110813.mail.gq1.yahoo.com [67.195.13.236])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n32F2SQY019633
7, 24 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 10:02:29 -0500
7, 24 -- Received: (qmail 41994 invoked by uid 60001); 2 Apr 2009 15:02:28 -0000
7, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238684548; bh=McUspaJt0F72svXlOz4Uf9XAUuBIx9lPvqWPqxtJfiA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=kKrlHnTobuN4b1vLnfgwybYbKUzMrWdIQMJx5hJ4zgVX6QcOFB3FDDCzatBlXdSSNtRl3QczNAdgi8Rrhy6l1CfmHFhWKxLflphNYcGpRfXH4B4B5cFD4cKl05E8OWuv62ETHIwskwRYrGQ4//PkxAQbVeErHxwJCHOVIzvzF9s=
7, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 24 -- s=s1024; d=yahoo.com;
7, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
7, 24 -- b=jCA8xUTWscpRBxoDdYZwKTauYV3OO+eV6292PFoAsNZrB2QdnYpasY8g0LlPqVzCU0qNttXLHEajq9IsKyLZYP+ft53K++hRjjUN8JmsJzBYxbn/49eNc4myZkl/U3LlCZAnPXZE3khV58qW2UwL0eK5FiPfNamOcz/gq03UyqI=;
7, 24 -- Message-ID: {302907.41673.qm-at-web110813.mail.gq1.yahoo.com}
7, 24 -- X-YMail-OSG: 3QNqEqUVM1li3pitfgrQcPpBGqdzsW3XZB4EdSKO.Fm3zUEpo5epPoV.5NUx7yPP3orCszsUKtibJcM6BqHO2VHFzTd3FXuvPSXHmiGNw8p7g.hzAFxYUb96pXelotKTcZniUWIvyS8ePMV.amN1.JKDOYlk5JKiEwIupWO2sJ1LLcLv5Bn.TaOjmVAKZdareENe7WljkRwuO2WnYLqSWJq3KfkAghDODY81YaYOrHJTA0w3gf6_HzYPlOHVaMNhBfPXSxwUKHHMIb9uGmirmRX8Px95oi1i_6Mu1WGrMgTRKoc41gJIPg--
7, 24 -- Received: from [80.122.101.100] by web110813.mail.gq1.yahoo.com via HTTP; Thu, 02 Apr 2009 08:02:28 PDT
7, 24 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
7, 24 -- References: {200904021026.n32AQE2b001090-at-ns.microscopy.com}
7, 24 -- Date: Thu, 2 Apr 2009 08:02:28 -0700 (PDT)
7, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 24 -- Subject: Re: [Microscopy] Re: gold target: second life?
7, 24 -- To: microscopy-at-microscopy.com
7, 24 -- In-Reply-To: {200904021026.n32AQE2b001090-at-ns.microscopy.com}
7, 24 -- MIME-Version: 1.0
7, 24 -- Content-Type: text/plain; charset=iso-8859-1
7, 24 -- Content-Transfer-Encoding: 8bit
7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n32F2SQY019633
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 2 Apr 2009 10:08:33 -0500
Subject: [Microscopy] gold target: second life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For those in north America, I believe that Abe Dayani of Recycling Systems Inc. will accept old targets and give credit toward the purchase of new ones. They are also a great source targets made to order at lower cost than OEM targets.

No connection with them except satisfied customer for many years.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Thursday, April 02, 2009 10:04 AM
To: Tindall, Randy D.


Thank you to all for your answers/comments!
We have only 1 target, it is too few for anybody to care about this amount alone.
But I was directed to a company in Germany called "BALTIC Präparation" which recycles the gold and deduces a part of its value from the new target:
I give this information in case anybody else need it too.

Regards,

Stephane





==============================Original Headers==============================
7, 24 -- From nizets2-at-yahoo.com Thu Apr 2 10:02:29 2009
7, 24 -- Received: from web110813.mail.gq1.yahoo.com (web110813.mail.gq1.yahoo.com [67.195.13.236])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n32F2SQY019633
7, 24 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 10:02:29 -0500
7, 24 -- Received: (qmail 41994 invoked by uid 60001); 2 Apr 2009 15:02:28 -0000
7, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238684548; bh=McUspaJt0F72svXlOz4Uf9XAUuBIx9lPvqWPqxtJfiA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=kKrlHnTobuN4b1vLnfgwybYbKUzMrWdIQMJx5hJ4zgVX6QcOFB3FDDCzatBlXdSSNtRl3QczNAdgi8Rrhy6l1CfmHFhWKxLflphNYcGpRfXH4B4B5cFD4cKl05E8OWuv62ETHIwskwRYrGQ4//PkxAQbVeErHxwJCHOVIzvzF9s=
7, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 24 -- s=s1024; d=yahoo.com;
7, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
7, 24 -- b=jCA8xUTWscpRBxoDdYZwKTauYV3OO+eV6292PFoAsNZrB2QdnYpasY8g0LlPqVzCU0qNttXLHEajq9IsKyLZYP+ft53K++hRjjUN8JmsJzBYxbn/49eNc4myZkl/U3LlCZAnPXZE3khV58qW2UwL0eK5FiPfNamOcz/gq03UyqI=;
7, 24 -- Message-ID: {302907.41673.qm-at-web110813.mail.gq1.yahoo.com}
7, 24 -- X-YMail-OSG: 3QNqEqUVM1li3pitfgrQcPpBGqdzsW3XZB4EdSKO.Fm3zUEpo5epPoV.5NUx7yPP3orCszsUKtibJcM6BqHO2VHFzTd3FXuvPSXHmiGNw8p7g.hzAFxYUb96pXelotKTcZniUWIvyS8ePMV.amN1.JKDOYlk5JKiEwIupWO2sJ1LLcLv5Bn.TaOjmVAKZdareENe7WljkRwuO2WnYLqSWJq3KfkAghDODY81YaYOrHJTA0w3gf6_HzYPlOHVaMNhBfPXSxwUKHHMIb9uGmirmRX8Px95oi1i_6Mu1WGrMgTRKoc41gJIPg--
7, 24 -- Received: from [80.122.101.100] by web110813.mail.gq1.yahoo.com via HTTP; Thu, 02 Apr 2009 08:02:28 PDT
7, 24 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
7, 24 -- References: {200904021026.n32AQE2b001090-at-ns.microscopy.com}
7, 24 -- Date: Thu, 2 Apr 2009 08:02:28 -0700 (PDT)
7, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 24 -- Subject: Re: [Microscopy] Re: gold target: second life?
7, 24 -- To: microscopy-at-microscopy.com
7, 24 -- In-Reply-To: {200904021026.n32AQE2b001090-at-ns.microscopy.com}
7, 24 -- MIME-Version: 1.0
7, 24 -- Content-Type: text/plain; charset=iso-8859-1
7, 24 -- Content-Transfer-Encoding: 8bit
7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n32F2SQY019633
==============================End of - Headers==============================



==============================Original Headers==============================
21, 29 -- From TindallR-at-missouri.edu Thu Apr 2 10:08:33 2009
21, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32F8WgT027200
21, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 10:08:33 -0500
21, 29 -- X-IronPort-Anti-Spam-Filtered: true
21, 29 -- X-IronPort-Anti-Spam-Result: ApsEAOtx1EnRauUp/2dsb2JhbACNG7NiAQmHHYhNgkqBMgY
21, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
21, 29 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 02 Apr 2009 10:08:32 -0500
21, 29 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
21, 29 -- Thu, 2 Apr 2009 10:08:31 -0500
21, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 29 -- Content-class: urn:content-classes:message
21, 29 -- MIME-Version: 1.0
21, 29 -- Content-Type: text/plain;
21, 29 -- charset="iso-8859-1"
21, 29 -- Subject: RE: [Microscopy] gold target: second life?
21, 29 -- Date: Thu, 2 Apr 2009 10:08:23 -0500
21, 29 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FA5-at-UM-XMAIL08.um.umsystem.edu}
21, 29 -- In-Reply-To: {200904021503.n32F3l2l021242-at-ns.microscopy.com}
21, 29 -- X-MS-Has-Attach:
21, 29 -- X-MS-TNEF-Correlator:
21, 29 -- Thread-Topic: [Microscopy] gold target: second life?
21, 29 -- Thread-Index: AcmzpDll5j4KlePMTFKmibXwLTcJHAAAE0Wg
21, 29 -- References: {200904021503.n32F3l2l021242-at-ns.microscopy.com}
21, 29 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
21, 29 -- To: {microscopy-at-microscopy.com}
21, 29 -- X-OriginalArrivalTime: 02 Apr 2009 15:08:31.0625 (UTC) FILETIME=[E2B45F90:01C9B3A4]
21, 29 -- Content-Transfer-Encoding: 8bit
21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n32F8WgT027200
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 2 Apr 2009 10:15:50 -0500
Subject: [Microscopy] Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That should have been REFINING Systems Inc., not Recycling. My bad.

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
5, 27 -- From TindallR-at-missouri.edu Thu Apr 2 10:15:49 2009
5, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32FFni5014879
5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 10:15:49 -0500
5, 27 -- X-IronPort-Anti-Spam-Filtered: true
5, 27 -- X-IronPort-Anti-Spam-Result: ApoEABhz1EnRauUo/2dsb2JhbADAdAEJhx2ITYJKgTIG
5, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
5, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 02 Apr 2009 10:15:49 -0500
5, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
5, 27 -- Thu, 2 Apr 2009 10:15:43 -0500
5, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 27 -- Content-class: urn:content-classes:message
5, 27 -- MIME-Version: 1.0
5, 27 -- Content-Type: text/plain;
5, 27 -- charset="us-ascii"
5, 27 -- Subject: Correction
5, 27 -- Date: Thu, 2 Apr 2009 10:15:15 -0500
5, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FA6-at-UM-XMAIL08.um.umsystem.edu}
5, 27 -- X-MS-Has-Attach:
5, 27 -- X-MS-TNEF-Correlator:
5, 27 -- Thread-Topic: Correction
5, 27 -- Thread-Index: AcmzpdNk49omzGUjS3yOCtwuBAJ1mA==
5, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
5, 27 -- To: {microscopy-at-microscopy.com}
5, 27 -- X-OriginalArrivalTime: 02 Apr 2009 15:15:43.0683 (UTC) FILETIME=[E43B3130:01C9B3A5]
5, 27 -- Content-Transfer-Encoding: 8bit
5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n32FFni5014879
==============================End of - Headers==============================




From: lisa-at-glosuntech.com
Date: Thu, 2 Apr 2009 14:50:39 -0500
Subject: [Microscopy] Looking for an used TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listserver members,

Please allow me to introduce myself. My name is Lisa Chen from Glosun Tech LLC.


We are currently building our thin film solar cell lab which requires a TEM (Transmission Electron Microscope) to do the quality control. If you have an used TEM that you wish to give away, please let us know, our email is lisa-at-glosuntech.com. We will be glad to come and give it a new home. We will pay all shipping and packing costs.

Glosun Tech LLC is a start-up small business company, which manufactures thin film CIGS and dye sensitized solar cells. We also provide accurate performance and reliability testing for photovoltaic materials and devices including Si, GaAs, CdTe, CIGS, dye-sensitized, and organic solar cells.

We really appreciate your help and time.




Best regards,



Lisa Chen

Glosuntech LLC

==============================Original Headers==============================
12, 31 -- From lisa-at-glosuntech.com Thu Apr 2 14:50:39 2009
12, 31 -- Received: from mta4.brinkster.com (mta4.brinkster.com [65.182.109.73])
12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32Joc2F010643
12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 14:50:39 -0500
12, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 46EEB5ED962
12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 15:50:36 -0400 (EDT)
12, 31 -- X-Virus-Scanned: amavisd-new at
12, 31 -- X-Spam-Flag: NO
12, 31 -- X-Spam-Score: -2.499
12, 31 -- X-Spam-Level:
12, 31 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=5
12, 31 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
12, 31 -- Received: from mta4.brinkster.com ([127.0.0.1])
12, 31 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024)
12, 31 -- with ESMTP id lPZssg+8Yjr2 for {Microscopy-at-Microscopy.Com} ;
12, 31 -- Thu, 2 Apr 2009 15:50:31 -0400 (EDT)
12, 31 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217])
12, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 1BD5C5EDBB8
12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 15:50:31 -0400 (EDT)
12, 31 -- Date: Thu, 2 Apr 2009 15:50:32 -0400 (EDT)
12, 31 -- From: lisa-at-glosuntech.com
12, 31 -- To: Microscopy-at-Microscopy.Com
12, 31 -- Message-ID: {26215955.131111238701832717.JavaMail.root-at-mail10a.brinkster.com}
12, 31 -- In-Reply-To: {27147398.130841238701573423.JavaMail.root-at-mail10a.brinkster.com}
12, 31 -- Subject: Looking for an used TEM
12, 31 -- MIME-Version: 1.0
12, 31 -- Content-Type: text/plain; charset=utf-8
12, 31 -- Content-Transfer-Encoding: 7bit
12, 31 -- X-Originating-IP: [75.140.108.220]
12, 31 -- X-Mailer: Zimbra 5.0.8_GA_2463.RHEL4 (ZimbraWebClient - FF3.0 (Win)/5.0.8_GA_2462.RHEL4_64)
==============================End of - Headers==============================




From: bart-at-cannonmicroprobe.com
Date: Thu, 2 Apr 2009 17:48:18 -0500
Subject: [Microscopy] viaWWW: Seeking Used DigiSEM Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bart-at-cannonmicroprobe.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bart-at-cannonmicroprobe.com
Name: Bart Cannon

Organization: Cannon Microprobe

Title-Subject: [Filtered] Seeking Used DigiSEM Board

Question: Hi,

My DigiSEM digital image acquisition ISA style computer board
recently failed. It gave more than a decade of good service.

I would very much like to obtain a working board, and would pay a
good price for one.

They were manufactured by Elmdas in the mid to late 90s.

Hundreds of these boards were in use. John L. Best was the designer.

Thank you.

Login Host: 67.170.22.28
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Apr 2 17:48:18 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32MmHuB000787
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 17:48:18 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240800c5faf11e673b-at-[206.69.208.22]}
11, 11 -- Date: Thu, 2 Apr 2009 17:48:16 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: bart-at-cannonmicroprobe.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Seeking Used DigiSEM Board
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tom-at-TomKaye.com
Date: Thu, 2 Apr 2009 18:07:30 -0500
Subject: [Microscopy] viaWWW: Seeking Used DigiSEM Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You might give these guys a call and see if they can repair the board. They
specialize in component level repair. They keep the FAA's junk from the 60's
running.
SMH ELECTRONICS CO
508-291-7447

Usual disclaimers,

Tom Kaye



-----Original Message-----
X-from: bart-at-cannonmicroprobe.com [mailto:bart-at-cannonmicroprobe.com]
Sent: Thursday, April 02, 2009 3:53 PM
To: tom-at-tomkaye.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bart-at-cannonmicroprobe.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bart-at-cannonmicroprobe.com
Name: Bart Cannon

Organization: Cannon Microprobe

Title-Subject: [Filtered] Seeking Used DigiSEM Board

Question: Hi,

My DigiSEM digital image acquisition ISA style computer board
recently failed. It gave more than a decade of good service.

I would very much like to obtain a working board, and would pay a
good price for one.

They were manufactured by Elmdas in the mid to late 90s.

Hundreds of these boards were in use. John L. Best was the designer.

Thank you.

Login Host: 67.170.22.28
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Apr 2 17:48:18 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n32MmHuB000787
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009
17:48:18 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240800c5faf11e673b-at-[206.69.208.22]}
11, 11 -- Date: Thu, 2 Apr 2009 17:48:16 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: bart-at-cannonmicroprobe.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Seeking Used DigiSEM Board
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
22, 22 -- From tom-at-TomKaye.com Thu Apr 2 18:07:30 2009
22, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com [66.102.127.13])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32N7SmO015257
22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 18:07:29 -0500
22, 22 -- Received: from TKdual ([67.134.109.68]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959);
22, 22 -- Thu, 2 Apr 2009 18:07:25 -0500
22, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com}
22, 22 -- To: {microscopy-at-microscopy.com}
22, 22 -- Subject: RE: [Microscopy] viaWWW: Seeking Used DigiSEM Board
22, 22 -- Date: Thu, 2 Apr 2009 16:05:21 -0700
22, 22 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGCEADAFAB.tom-at-tomkaye.com}
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain;
22, 22 -- charset="iso-8859-1"
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Priority: 3 (Normal)
22, 22 -- X-MSMail-Priority: Normal
22, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0)
22, 22 -- Importance: Normal
22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512
22, 22 -- In-Reply-To: {200904022252.n32MqkKP006890-at-ns.microscopy.com}
22, 22 -- X-OriginalArrivalTime: 02 Apr 2009 23:07:26.0019 (UTC) FILETIME=[C9BCF130:01C9B3E7]
==============================End of - Headers==============================




From: delannoy-at-jhmi.edu
Date: Fri, 3 Apr 2009 14:30:36 -0500
Subject: [Microscopy] lr gold precip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To the knowing,
We have a precipitation problem with mouse liver fixed by immersion with 2%PLP and then processed for LR Gold embedding (minus tannic acid and uranyl acetate)dehydrated in ETOH. It looks like traditional Pb pepper, but none was used. The attached jpeg is cut then immediately viewed (no labelling or staining). Any thoughts?

M Delannoy

==============================Original Headers==============================
2, 27 -- From delannoy-at-jhmi.edu Fri Apr 3 14:30:35 2009
2, 27 -- Received: from ipex2.johnshopkins.edu (ipex2.johnshopkins.edu [162.129.8.151])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n33JUYcZ006899
2, 27 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 14:30:35 -0500
2, 27 -- X-IronPort-AV: E=Sophos;i="4.39,320,1235970000";
2, 27 -- d="scan'208";a="177815969"
2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
2, 27 -- by ipex2.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 03 Apr 2009 15:30:33 -0400
2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by jesmail.johnshopkins.edu
2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006))
2, 27 -- with ESMTP id {0KHJ00453I6Z6WK0-at-jesmail.johnshopkins.edu} for
2, 27 -- microscopy-at-microscopy.com; Fri, 03 Apr 2009 15:30:35 -0400 (EDT)
2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59])
2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Fri, 03 Apr 2009 15:30:35 -0400
2, 27 -- Date: Fri, 03 Apr 2009 15:30:35 -0400
2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
2, 27 -- Subject: lr gold precip
2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 27 -- Message-id: {f6b6abccbe1.49d62b9b-at-johnshopkins.edu}
2, 27 -- MIME-version: 1.0
2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006)
2, 27 -- Content-type: text/plain; charset=us-ascii
2, 27 -- Content-language: en
2, 27 -- Content-transfer-encoding: 7BIT
2, 27 -- Content-disposition: inline
2, 27 -- X-Accept-Language: en
2, 27 -- Priority: normal
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Fri, 3 Apr 2009 15:54:48 -0500
Subject: [Microscopy] Zeiss Photomik III

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I'm still having trouble trying to find where the ground wire (at least I think it's a ground wire) attaches to the bulb assembly of the HBO100 on my Photomik III. This thing might not even attach to the bulb assembly-but I can't remember.

I don't have access to a server with which to post images but if you contact me off list I can send you some .jpg images I made of the little beasty.

Even the Zeiss people are having a tough time with this one. All the Zeiss documentation shows a completely different set up from the one I have, lucky me.

Put on you thinking caps and please try to help me solve my puzzle.

Let this be a lesson to all-either put it back together while awaiting parts or take detailed notes. You never know when you have to quickly vacate a facility and then have your brain vacate during the interim.

Thanks,

Paula the forgetful ;-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
13, 25 -- From vapatpxs-at-yahoo.com Fri Apr 3 15:54:48 2009
13, 25 -- Received: from n23b.bullet.mail.mud.yahoo.com (n23b.bullet.mail.mud.yahoo.com [68.142.206.142])
13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n33KslTZ023032
13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 15:54:47 -0500
13, 25 -- Received: from [68.142.200.227] by n23.bullet.mail.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000
13, 25 -- Received: from [68.142.201.243] by t8.bullet.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000
13, 25 -- Received: from [127.0.0.1] by omp404.mail.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000
13, 25 -- X-Yahoo-Newman-Property: ymail-3
13, 25 -- X-Yahoo-Newman-Id: 273625.7841.bm-at-omp404.mail.mud.yahoo.com
13, 25 -- Received: (qmail 7730 invoked by uid 60001); 3 Apr 2009 20:54:46 -0000
13, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238792086; bh=u5aPJqnN7bFp8By7x99KWHprApqHmJONBrbvrNGKFEI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=lRkxVAzd+BBTH4PSO//3HH7ar07PbzcXbvRu0O1Rpk/G7hbfe21qeH0W660REoT3euplPo+Db3tVm3cuQ8cNKYbWVq/T8Etqy11c0+yKBzD8o9IhaDGzGJaxomdwuxmiLub/IcgTSzKYI5gNGuvrG2WLDdxxxrrTEtenaNMc9U8=
13, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 25 -- s=s1024; d=yahoo.com;
13, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 25 -- b=oNLwbpiknFnfEqqEq+x7eVVQ0eToUepBZmeiKifTL44sEqhhtsUaEOTFlU+iFMxt4JTsw1Bn+eyaSc+qEgoxvsnalAMZmDWMYbkEvWpzvjSWLCFs7DciHsSemiFUunFIUvLfOvhlGFnfbHelSMBMVnin4oe0zDY3ETDpNbCSzec=;
13, 25 -- Message-ID: {753201.7507.qm-at-web46112.mail.sp1.yahoo.com}
13, 25 -- X-YMail-OSG: C2Nt_UwVM1nJfGZuQ8rdPHHn8DWDBJWXszPqGqIiZyHkii4Nwmk15P.NpsYYuVOrFjFE78GUZpCY6D1w6jCLZOvxivYtQ1Js_wjaJs.MeN_Vtb7xKaJc38TncWVlq3jJQhZ.bzqrtDkJxNLvN02WeuCboYm1mXhRKknZs_N0sG8Byihtd6pkbJXTP.GJBJc9cylnP3S6xHBbk9IAYoIUjRHylxnzVNPTNc2BmaansgLOmjQ2dWBXh3rCIC4JEx0cLpqGs479xE9P5LrNuFg-
13, 25 -- Received: from [132.239.85.200] by web46112.mail.sp1.yahoo.com via HTTP; Fri, 03 Apr 2009 13:54:46 PDT
13, 25 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
13, 25 -- Date: Fri, 3 Apr 2009 13:54:46 -0700 (PDT)
13, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 25 -- Subject: Zeiss Photomik III
13, 25 -- To: MSA BB {Microscopy-at-microscopy.com}
13, 25 -- MIME-Version: 1.0
13, 25 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 3 Apr 2009 16:26:01 -0500
Subject: [Microscopy] FFT with digital TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have a question about TEM digital cameras.
Are they capable of displaying live FFT images for stigmation and
focusing? Do this capability come usually as option or built in basic
package?

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
8, 23 -- From DusevichV-at-umkc.edu Fri Apr 3 16:26:00 2009
8, 23 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n33LQ0M5005264
8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 16:26:00 -0500
8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Fri, 3 Apr 2009 16:25:59 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: FFT with digital TEM camera
8, 23 -- Date: Fri, 3 Apr 2009 16:25:58 -0500
8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB813-at-KC-MSX1.kc.umkc.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: FFT with digital TEM camera
8, 23 -- Thread-Index: Acm0osfdDP1JWk8gQlGXKZa4ieaK0g==
8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 03 Apr 2009 21:25:59.0306 (UTC) FILETIME=[C82FFEA0:01C9B4A2]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n33LQ0M5005264
==============================End of - Headers==============================




From: edlast-at-pacbell.net
Date: Fri, 3 Apr 2009 17:56:09 -0500
Subject: [Microscopy] FFT with digital TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Im using DM with has Standard FFT and Live FFT capabilities.

==============================Original Headers==============================
2, 21 -- From edlast-at-pacbell.net Fri Apr 3 17:56:09 2009
2, 21 -- Received: from web180204.mail.gq1.yahoo.com (web180204.mail.gq1.yahoo.com [67.195.13.164])
2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n33Mu80u021254
2, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 17:56:08 -0500
2, 21 -- Received: (qmail 20255 invoked by uid 60001); 3 Apr 2009 22:56:06 -0000
2, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1238799366; bh=S70r9YKrkFxMKdk3d9Ql9iNnhd2H0ef1CS36+DSb3kA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=UcnlwI68dkWpAzVodBWptsStB6EuZtVe0brw8mx9SVkTsmKYLY5PeD4W7zUWwcdMmi3itDONs3h62lyXZbQpyKSfV0Ikv1ytvu4MXpVhKH219AeC1XE38PeNJUD44HKrL4EUhrFKfPil6Y4tzReGMPEsb/VRmRnOlzmj3PGD+GI=
2, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
2, 21 -- s=s1024; d=pacbell.net;
2, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type;
2, 21 -- b=1tgoQlOmpFoM5s9h0hXvm5tJW4nqEaznpyUUeG8xmhTb0zvcE71cYuER7TdxnoxSxYzfBqlEd0vUg/TdrIyayvkoL3o7+ZsEGgBSnV+i60FUtjjjeRlmbNsFZIsj7rXu2XiDeDM3fYVFYEvHToGCzrEhiyRyFUeebHvuTtD9jjI=;
2, 21 -- Message-ID: {928963.19382.qm-at-web180204.mail.gq1.yahoo.com}
2, 21 -- X-YMail-OSG: AAeiNwoVM1mjZGgo.tZebcfwEckCxu3iIRpzmz40wzaAByiLpHK66kZcZbg_KckFNqnImRkMrzMyeemN1F1lmajgzMwR8eRvyUJitIm869WeBOA_cjLyX5t8tREyPZZYLvXU0SNe_x3CXKxCJca1d9yp0N_kW5eA8oQbfBj6W._670ObadkxorsjRLZAEBWGoN48Lwvqg1fb95GBWp46JxcuWvuzzjAeIC.o4IO.EN3wWZE9XeCGeBWZ_N91B.Kd6X_kZO52yR7N2itM
2, 21 -- Received: from [209.3.42.11] by web180204.mail.gq1.yahoo.com via HTTP; Fri, 03 Apr 2009 15:56:06 PDT
2, 21 -- X-Mailer: YahooMailWebService/0.7.289.1
2, 21 -- Date: Fri, 3 Apr 2009 15:56:06 -0700 (PDT)
2, 21 -- From: ed last {edlast-at-pacbell.net}
2, 21 -- Reply-To: edlast-at-pacbell.net
2, 21 -- Subject: FFT with digital TEM camera
2, 21 -- To: microscopy-at-microscopy.com
2, 21 -- MIME-Version: 1.0
2, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: frankehrenfeld-at-iatl.com
Date: Fri, 3 Apr 2009 18:32:25 -0500
Subject: [Microscopy] viaWWW: Used EDS Wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both frankehrenfeld-at-iatl.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: frankehrenfeld-at-iatl.com
Name: Frank Ehrenfeld

Organization: IATL

Title-Subject: [Filtered] Used EDS

Question: We have two Kevex Delta Class EDS. We are looking for used
EDS of same vintage or other used EDXA systems for our two TEMs.
Please respond to the email above.

Login Host: 146.145.252.34
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Apr 3 18:32:25 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n33NWOee003238
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 18:32:25 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c5fc4cf0edf6-at-[206.69.208.22]}
6, 11 -- Date: Fri, 3 Apr 2009 18:32:23 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: frankehrenfeld-at-iatl.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Used EDS Wanted
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mdelann1-at-jhmi.edu
Date: Fri, 3 Apr 2009 18:32:47 -0500
Subject: [Microscopy] viaWWW: LR Gold precipitate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mdelann1-at-jhmi.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mdelann1-at-jhmi.edu
Name: Michael Delannoy

Organization: JHMI Microscope Facility

Title-Subject: [Filtered] LR Gold precipitate

Question: To the knowing,
We have a precipitation problem with mouse liver fixed by immersion
with 2%PLP and then processed for LR Gold embedding (minus tannic
acid and uranyl acetate)dehydrated in ETOH. It looks like
traditional Pb pepper, but none was used. The attached jpeg is cut
then immediately viewed (no labelling or staining). Any thoughts?

M Delannoy

Login Host: 162.129.251.18
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Apr 3 18:32:47 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n33NWkvL003658
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 18:32:46 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c5fc4d0ff547-at-[206.69.208.22]}
7, 11 -- Date: Fri, 3 Apr 2009 18:32:45 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: mdelann1-at-jhmi.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: LR Gold precipitate
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Fri, 3 Apr 2009 20:54:33 -0500
Subject: [Microscopy] Re: viaWWW: Used EDS Wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank
Numonyx recently warehoused a 30 square mm Noran detector that fit a
JEOL2010. Since IATL is a commercial lab, you would need to bid for
it. Donations are for universties only. Contact Caroline Ayre at 4087652348
.
Sent from my iPhone

On Apr 3, 2009, at 4:42 PM, frankehrenfeld-at-iatl.com wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---
} ---
} ---------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both frankehrenfeld-at-iatl.com as well as the
} MIcroscopy Listserver
} ---
} ---
} ---------------------------------------------------------------------
}
} Email: frankehrenfeld-at-iatl.com
} Name: Frank Ehrenfeld
}
} Organization: IATL
}
} Title-Subject: [Filtered] Used EDS
}
} Question: We have two Kevex Delta Class EDS. We are looking for used
} EDS of same vintage or other used EDXA systems for our two TEMs.
} Please respond to the email above.
}
} Login Host: 146.145.252.34
} ---
} ---
} ---------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Fri Apr 3 18:32:25 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22
} ])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n33NWOee003238
} 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009
} 18:32:25 -0500
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240800c5fc4cf0edf6-at-[206.69.208.22]}
} 6, 11 -- Date: Fri, 3 Apr 2009 18:32:23 -0500
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: frankehrenfeld-at-iatl.com (by way of
} MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: Used EDS Wanted
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
4, 44 -- From jmardinly-at-gmail.com Fri Apr 3 20:54:32 2009
4, 44 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.180])
4, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n341sWgt002564
4, 44 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 20:54:32 -0500
4, 44 -- Received: by wa-out-1112.google.com with SMTP id j5so738254wah.2
4, 44 -- for {Microscopy-at-microscopy.com} ; Fri, 03 Apr 2009 18:54:32 -0700 (PDT)
4, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 44 -- d=gmail.com; s=gamma;
4, 44 -- h=domainkey-signature:received:received:message-id:from:to
4, 44 -- :in-reply-to:content-type:content-transfer-encoding:x-mailer
4, 44 -- :mime-version:subject:date:references;
4, 44 -- bh=Vkl7TP6hwQ0iLUbTLzoiSfBaC7PLkhQ35g3x5z/oGj4=;
4, 44 -- b=uEovGnkO5XdT9ebPCy5sJqNmcPE5aiJpd5nxf9puC2hVNyFyFl38F3h1pKsYhUO9fn
4, 44 -- YW5yrpjec76MjoW378wtC1zEtWSWOfg3s8pc2iHHAVZ215oQ7uov3rta8PW61biq30SQ
4, 44 -- 01KIbBNOjWXAan3M1l/mB6SRHhQysk8G33YJs=
4, 44 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 44 -- d=gmail.com; s=gamma;
4, 44 -- h=message-id:from:to:in-reply-to:content-type
4, 44 -- :content-transfer-encoding:x-mailer:mime-version:subject:date
4, 44 -- :references;
4, 44 -- b=leihIKc9cYDRfSXNoo7vWMwQ6AIqdPngEydPK+or48Es7VaCJBTLrlpYRU6jHWgJky
4, 44 -- iXN66GDTih7ggE/cYSD4JLbHqRVdbWVhQsPetmnTXxs1p1OoyztCQ7OvmAhq98a+AC/R
4, 44 -- FIj8mgmPX1rRECe0NmDkrSPF3UlOp+egv/DDs=
4, 44 -- Received: by 10.115.18.3 with SMTP id v3mr908141wai.141.1238810072047;
4, 44 -- Fri, 03 Apr 2009 18:54:32 -0700 (PDT)
4, 44 -- Received: from ?192.168.1.68? (75-25-131-32.lightspeed.sjcpca.sbcglobal.net [75.25.131.32])
4, 44 -- by mx.google.com with ESMTPS id l37sm2811677waf.3.2009.04.03.18.54.31
4, 44 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 44 -- Fri, 03 Apr 2009 18:54:31 -0700 (PDT)
4, 44 -- Message-Id: {C6FD37BA-6428-4493-BFC8-94CD5C591FE5-at-gmail.com}
4, 44 -- From: John Mardinly {jmardinly-at-gmail.com}
4, 44 -- To: "frankehrenfeld-at-iatl.com" {frankehrenfeld-at-iatl.com} ,
4, 44 -- MSA Listserver {Microscopy-at-microscopy.com}
4, 44 -- In-Reply-To: {200904032342.n33Nggt7030700-at-ns.microscopy.com}
4, 44 -- Content-Type: text/plain;
4, 44 -- charset=us-ascii;
4, 44 -- format=flowed;
4, 44 -- delsp=yes
4, 44 -- Content-Transfer-Encoding: 7bit
4, 44 -- X-Mailer: iPhone Mail (5G77)
4, 44 -- Mime-Version: 1.0 (iPhone Mail 5G77)
4, 44 -- Subject: Re: [Microscopy] viaWWW: Used EDS Wanted
4, 44 -- Date: Fri, 3 Apr 2009 18:54:26 -0700
4, 44 -- References: {200904032342.n33Nggt7030700-at-ns.microscopy.com}
==============================End of - Headers==============================




From: Elliott-at-arizona.edu
Date: Fri, 3 Apr 2009 21:56:08 -0500
Subject: [Microscopy] Re: FFT with digital TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My AMT XR-40 camera does FFT for stigmation and focusing. It is part
of the standard camera software.
David


On Apr 3, 2009, at 2:29 PM, DusevichV-at-umkc.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi,
}
} I have a question about TEM digital cameras.
} Are they capable of displaying live FFT images for stigmation and
} focusing? Do this capability come usually as option or built in basic
} package?
}
} Thank you,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} ==============================Original
} Headers==============================
} 8, 23 -- From DusevichV-at-umkc.edu Fri Apr 3 16:26:00 2009
} 8, 23 -- Received: from KC-MSXPROTO2.kc.umkc.edu
} (smtp.exchange.umkc.edu [134.193.143.155])
} 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n33LQ0M5005264
} 8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 16:26:00
} -0500
} 8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-
} MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 23 -- Fri, 3 Apr 2009 16:25:59 -0500
} 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 8, 23 -- Content-class: urn:content-classes:message
} 8, 23 -- MIME-Version: 1.0
} 8, 23 -- Content-Type: text/plain;
} 8, 23 -- charset="us-ascii"
} 8, 23 -- Subject: FFT with digital TEM camera
} 8, 23 -- Date: Fri, 3 Apr 2009 16:25:58 -0500
} 8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB813-at-KC-MSX1.kc.umkc.edu
} }
} 8, 23 -- X-MS-Has-Attach:
} 8, 23 -- X-MS-TNEF-Correlator:
} 8, 23 -- Thread-Topic: FFT with digital TEM camera
} 8, 23 -- Thread-Index: Acm0osfdDP1JWk8gQlGXKZa4ieaK0g==
} 8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 23 -- To: {Microscopy-at-microscopy.com}
} 8, 23 -- X-OriginalArrivalTime: 03 Apr 2009 21:25:59.0306 (UTC)
} FILETIME=[C82FFEA0:01C9B4A2]
} 8, 23 -- Content-Transfer-Encoding: 8bit
} 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n33LQ0M5005264
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
5, 22 -- From Elliott-at-arizona.edu Fri Apr 3 21:56:08 2009
5, 22 -- Received: from mailgator.email.arizona.edu (gremlin.email.arizona.edu [128.196.133.171])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n342u7wG018759
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 3 Apr 2009 21:56:08 -0500
5, 22 -- Received: from mailgators_amavis (amavis6.email.arizona.edu [10.0.0.209])
5, 22 -- by mailgator.email.arizona.edu (Postfix) with ESMTP id 554A03F07BC5
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 3 Apr 2009 19:56:07 -0700 (MST)
5, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104])
5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D9677AC141C
5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 3 Apr 2009 19:56:04 -0700 (MST)
5, 22 -- Message-Id: {14F3FD4C-8F43-4E8F-933E-E59EBF36826C-at-arizona.edu}
5, 22 -- From: David Elliott {Elliott-at-arizona.edu}
5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com}
5, 22 -- In-Reply-To: {200904032129.n33LT4Tv009601-at-ns.microscopy.com}
5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
5, 22 -- Subject: Re: [Microscopy] FFT with digital TEM camera
5, 22 -- Date: Fri, 3 Apr 2009 19:56:04 -0700
5, 22 -- References: {200904032129.n33LT4Tv009601-at-ns.microscopy.com}
5, 22 -- X-Mailer: Apple Mail (2.930.3)
5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Sat, 4 Apr 2009 02:55:59 -0500
Subject: [Microscopy] commercial TEM services?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I would like to find out about commercial companies and their services
for TEM work: Types of samples, pricing, conditions etc. I mean
companies who would accept samples from anyone who need a TEM work
performed on their sample(s).

I have one critical question in mind: Given the fact that success rate
in bulk sample preparation for TEM is not 100%, can a commercial
company guaranty that they will produce TEM results from my sample?
Especially, when I have a limited amount of original sample to begin
with.


Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================

==============================Original Headers==============================
6, 31 -- From celikaktas-at-gmail.com Sat Apr 4 02:55:59 2009
6, 31 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com [209.85.220.166])
6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n347tws3007558
6, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 02:55:59 -0500
6, 31 -- Received: by fxm10 with SMTP id 10so1357029fxm.18
6, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 04 Apr 2009 00:55:58 -0700 (PDT)
6, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 31 -- d=gmail.com; s=gamma;
6, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
6, 31 -- :from:to:content-type:content-transfer-encoding;
6, 31 -- bh=bo9i9le75OMalTKZecPd10o0XhaGqRY87YNSLxgDcw4=;
6, 31 -- b=vRIo9iT3idgLeFG0GFbxX+nZJFMLX/8KRytq7spPRqYiKfpMENYYX7+EOKCqTqr7pA
6, 31 -- JHKQiGY2Cx1MtL816uuck8DycxECHXHNx5SoBIUKNJn9zbZsq6sa1m0rSKuzughXNrvH
6, 31 -- fcNxj4aw6bDUyyqenxpjGEx8bK7ME0loog3tY=
6, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
6, 31 -- d=gmail.com; s=gamma;
6, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
6, 31 -- :content-transfer-encoding;
6, 31 -- b=AlvkSmhAQIso6lrlP+CfCG+XNnt6f73AG0HVt7yDQ2TjIPDb9Ad6jmt7r9zepi1LFg
6, 31 -- NV99q3gSKl1vVgxRJwmaNNfshu8A11Ey5RibEegIsQmNC5QwGkC94YMKKABHCfhfFIo0
6, 31 -- suowRttM8OuFZEkJQ/CwDgYeqKPZMHH/0gFec=
6, 31 -- MIME-Version: 1.0
6, 31 -- Received: by 10.103.105.1 with SMTP id h1mr976731mum.13.1238831758094; Sat, 04
6, 31 -- Apr 2009 00:55:58 -0700 (PDT)
6, 31 -- Date: Sat, 4 Apr 2009 10:55:58 +0300
6, 31 -- Message-ID: {1075c5c10904040055o1a7ada4fn9ae320b463659de0-at-mail.gmail.com}
6, 31 -- Subject: commercial TEM services?
6, 31 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
6, 31 -- To: microscopy {Microscopy-at-microscopy.com}
6, 31 -- Content-Type: text/plain; charset=UTF-8
6, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Sat, 4 Apr 2009 05:18:59 -0500
Subject: [Microscopy] TEM work at Non-American Universities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I have another question regarding TEM work. I want to know how people
organize TEM work at university laboratories in other parts of the
World. I have completed my graduate study in United States.

In United States, we were given instructions to operate various sample
prep tools and TEM itself. And we, as graduate students, were expected
to prepare our samples and carry out our own TEM study. Which means we
make few mistakes and break few samples before we could finally
prepare a meaningful TEM sample.

I want to know how things are done in other parts of the World. Any
pros and cons when compared to American system?

Thanks a bunch,
Ayten.


===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================

==============================Original Headers==============================
7, 31 -- From celikaktas-at-gmail.com Sat Apr 4 05:18:59 2009
7, 31 -- Received: from mail-bw0-f159.google.com (mail-bw0-f159.google.com [209.85.218.159])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n34AIw9X025665
7, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 05:18:58 -0500
7, 31 -- Received: by bwz3 with SMTP id 3so1377683bwz.18
7, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 04 Apr 2009 03:18:58 -0700 (PDT)
7, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 31 -- d=gmail.com; s=gamma;
7, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
7, 31 -- :from:to:content-type:content-transfer-encoding;
7, 31 -- bh=/OpvR3jAwdK4zOSiKngCxpBECHK16kb6UhmuO8brVjo=;
7, 31 -- b=pPLqNI4EFY0LThgr9ByJCmTz+RejN+x7zyuexaANkQSJCJmHwl1anCGne2Ihq1ItlH
7, 31 -- aaKw31zAnwOZT3aRklQFAI2BnIDTqh+z26Yz/6vzzTrpeZb/vD3bo9S8uvXRVglPQ1bv
7, 31 -- 1DRC5XZaN7A/tsZ36vZo9kRqBoGt0EzzNdLhA=
7, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 31 -- d=gmail.com; s=gamma;
7, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
7, 31 -- :content-transfer-encoding;
7, 31 -- b=oGCwQwzS/J91RQE41QWY2minuEoje81IT5TqeZQ4qE1AwK1BsZNk5L1CDTzzxlPCnE
7, 31 -- qE0+hQ6lvkWbiMSaz73qqv7fvTZ+qCN7H3K4jLgq4kTjfPtAuqiI/I3VKHfuTBNwKpzk
7, 31 -- V3BD+8phD1GRzyVk0RVEiRvllqOr4GiQNFOKA=
7, 31 -- MIME-Version: 1.0
7, 31 -- Received: by 10.103.182.3 with SMTP id j3mr1003879mup.107.1238840337955; Sat,
7, 31 -- 04 Apr 2009 03:18:57 -0700 (PDT)
7, 31 -- Date: Sat, 4 Apr 2009 13:18:57 +0300
7, 31 -- Message-ID: {1075c5c10904040318y5a219389hc28fd1cd862566-at-mail.gmail.com}
7, 31 -- Subject: TEM work at Non-American Universities
7, 31 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
7, 31 -- To: microscopy {Microscopy-at-microscopy.com}
7, 31 -- Content-Type: text/plain; charset=UTF-8
7, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Sat, 4 Apr 2009 13:30:28 -0500
Subject: [Microscopy] Order

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

someone from Bowdoin College ordered a DVD but did not give me a
shipping address. If this person is listening, please email me.

--
Greg Erdos
Assistant Director Emeritus
Micanopy FL


==============================Original Headers==============================
3, 22 -- From gwe-at-ufl.edu Sat Apr 4 13:30:28 2009
3, 22 -- Received: from smtp.ufl.edu (smtp03.osg.ufl.edu [128.227.74.70])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n34IUSsd024782
3, 22 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 13:30:28 -0500
3, 22 -- Received: from osgjas01.cns.ufl.edu (osgjas01.cns.ufl.edu [128.227.74.131])
3, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n34IURdb000504
3, 22 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 14:30:27 -0400
3, 22 -- Message-ID: {284643213.211361238869827241.JavaMail.osg-at-osgjas01.cns.ufl.edu}
3, 22 -- Date: Sat, 4 Apr 2009 14:30:27 -0400 (EDT)
3, 22 -- From: greg erdos {gwe-at-ufl.edu}
3, 22 -- Reply-To: gwe-at-ufl.edu
3, 22 -- To: microscopy-at-microscopy.com
3, 22 -- Subject: Order
3, 22 -- MIME-Version: 1.0
3, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
3, 22 -- X-Originating-IP: 74.178.49.61 [74.178.49.61]
3, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-04-03_07:2009-04-02,2009-04-03,2009-04-04 signatures=0
3, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=1 spamscore=1 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0904040126
3, 22 -- X-Spam-Level: *
3, 22 -- X-UFL-Spam-Level: *
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Sat, 4 Apr 2009 13:31:25 -0500
Subject: [Microscopy] Order

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

someone from Bowdoin College ordered a DVD but did not give me a
shipping address. If this person is listening, please email me.

--
Greg Erdos
Assistant Director Emeritus
Micanopy FL


==============================Original Headers==============================
3, 22 -- From gwe-at-ufl.edu Sat Apr 4 13:31:25 2009
3, 22 -- Received: from smtp.ufl.edu (smtp04.osg.ufl.edu [128.227.74.71])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n34IVPiw026199
3, 22 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 13:31:25 -0500
3, 22 -- Received: from osgjas01.cns.ufl.edu (osgjas01.cns.ufl.edu [128.227.74.131])
3, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n34IVOK2013332
3, 22 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2009 14:31:24 -0400
3, 22 -- Message-ID: {1163136976.211411238869884566.JavaMail.osg-at-osgjas01.cns.ufl.edu}
3, 22 -- Date: Sat, 4 Apr 2009 14:31:24 -0400 (EDT)
3, 22 -- From: greg erdos {gwe-at-ufl.edu}
3, 22 -- Reply-To: gwe-at-ufl.edu
3, 22 -- To: microscopy-at-microscopy.com
3, 22 -- Subject: Order
3, 22 -- MIME-Version: 1.0
3, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
3, 22 -- X-Originating-IP: 74.178.49.61 [74.178.49.61]
3, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-04-03_07:2009-04-02,2009-04-03,2009-04-04 signatures=0
3, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=1 spamscore=1 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0904040126
3, 22 -- X-Spam-Level: *
3, 22 -- X-UFL-Spam-Level: *
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Sun, 5 Apr 2009 15:30:56 -0500
Subject: [Microscopy] FFT with digital TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I know that all digital TEM-Cameras from TVIPS Germany, and - I think, all - Gatan cameras have FFT capabilities, built into their own software, either live or with some delay (fractions of a second, or a second), depending on the speed of the camera, and the PC etc.
best regards,
Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
6, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Sun Apr 5 15:30:55 2009
6, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n35KUoG0018085
6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 15:30:54 -0500
6, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
6, 25 -- by localhost (Postfix) with SMTP id 1042AABA34
6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 22:30:57 +0200 (CEST)
6, 25 -- Received: from gwsmtp2.uni-regensburg.de (rrzgw6.rz.uni-regensburg.de [132.199.4.79])
6, 25 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 09176ABA08
6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 22:30:57 +0200 (CEST)
6, 25 -- Received: from uni-regensburg-3-MTA by gwsmtp2.uni-regensburg.de
6, 25 -- with Novell_GroupWise; Sun, 05 Apr 2009 22:30:47 +0200
6, 25 -- Message-Id: {49D93114020000540000CD7B-at-gwsmtp2.uni-regensburg.de}
6, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
6, 25 -- Date: Sun, 05 Apr 2009 22:30:44 +0200
6, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
6, 25 -- To: {Microscopy-at-Microscopy.Com}
6, 25 -- Subject: FFT with digital TEM camera
6, 25 -- References: {200904032256.n33Mup7a021612-at-ns.microscopy.com}
6, 25 -- In-Reply-To: {200904032256.n33Mup7a021612-at-ns.microscopy.com}
6, 25 -- Mime-Version: 1.0
6, 25 -- Content-Type: text/plain; charset=US-ASCII
6, 25 -- Content-Disposition: inline
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n35KUoG0018085
==============================End of - Headers==============================




From: dkoleary-at-verizon.net
Date: Sun, 5 Apr 2009 15:34:30 -0500
Subject: [Microscopy] PLM: Polarized Light Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New YorkMicroscopical Society
One Prospect Village Plaza
(66F Mount Prospect Avenue)
Clifton, NJ 07013
Bernard Friedman Memorial Workshop
 
Polarized Light Microscopy
 May 2, 9, 16 & 23, 2009
 
An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation,  The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation
 
The workshop will consist of four Consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch formally of Leica, Inc., Mary McCann of McCann Imaging, John Reffner of Smiths Detection and N.Y.M.S. Instructor Don O'Leary.
 
WHEN: May 2, 9, 16 & 23, 2009 from 10 A.M. to 4 P.M.
 
WHERE:
30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 470-8733
 
COST: $425 for N.Y.M.S. members, $455 for non-members (includes membership). Lunch and course materials are included. Checks made out to N.Y.M.S.
 
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.                   
 
HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663
FURTHER INFORMATION: Call D. O'Leary (201)368-8849 e-mail dkoleary-at-verizon.net
 
                                  PLEASE POST
--------------------------------------------------------------------------
                               Registration Form
                          Polarized Light Microscopy
 
N.Y.M.S. Member_________________ ($425)  Non-Member__________($455)
 
Name_____________________________________________________________
Address___________________________________________________________
Phone (W)_________________________(H)______________________________
e-mail________________________________________



==============================Original Headers==============================
4, 18 -- From dkoleary-at-verizon.net Sun Apr 5 15:34:30 2009
4, 18 -- Received: from web84003.mail.mud.yahoo.com (web84003.mail.mud.yahoo.com [68.142.206.173])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n35KYTCS021559
4, 18 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 15:34:29 -0500
4, 18 -- Received: (qmail 57221 invoked by uid 60001); 5 Apr 2009 20:34:29 -0000
4, 18 -- Message-ID: {647759.56470.qm-at-web84003.mail.mud.yahoo.com}
4, 18 -- X-YMail-OSG: jbOHv8MVM1mUgL2ZU7JiYRj.Pm8j3YeRLXW3GF91ardPZOGHkKlKQCvQ8gKlif8DUJJcKKJSLhgM9lrsu9Ux_1OxR7MD0ssaFMekepPT8XYEJ8f5zezPMm3D8F7powcedVNhyqoRch_rfDpC6BJlnIm7Z1lVSmNDkwTej9JSkBbNzkg4GsSA7uOBrwrOfp1ijD3A1lwHFlycB5yKmvNYDblOx.x5AhnqEHdaJ9MR3bfe_ElVH5W.pCuRF9boNNGOt6RWKnBSzvKydY3AHI57t6B53ffewfW7qBFBnnaXOEveHI9gYA--
4, 18 -- Received: from [71.122.23.82] by web84003.mail.mud.yahoo.com via HTTP; Sun, 05 Apr 2009 13:34:29 PDT
4, 18 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
4, 18 -- Date: Sun, 5 Apr 2009 13:34:29 -0700 (PDT)
4, 18 -- From: dkoleary-at-verizon.net
4, 18 -- Reply-To: dkoleary-at-verizon.net
4, 18 -- Subject: PLM: Polarized Light Microscopy Workshop
4, 18 -- To: Microscopy List server {Microscopy-at-Microscopy.Com}
4, 18 -- MIME-Version: 1.0
4, 18 -- Content-Type: text/plain; charset=iso-8859-1
4, 18 -- Content-Transfer-Encoding: 8bit
4, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n35KYTCS021559
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Mon, 6 Apr 2009 04:12:53 -0500
Subject: [Microscopy] TEM sample preparation? Re:TEM work at Non-American Universities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all who have responded to my previous inquiries.

Some university laboratories have one or two designated TEM operators
who operate TEM for everyone in some parts of the world. Commercial
TEM laboratories generally have specific areas of expertise.

Is there any university laboratory (a core facility) out there who has
a technician to do TEM sample preparation for graduate students?
Please, let me know if there is such a model out there? Given the
differences in sample preparation techniques of different kinds of
samples, I wonder how they do things? Do they have few technicians,
one for each kind of sample group (metals, biological samples,
ceramics etc.)?

Personally, I think there are numerous benefits to have graduate
students do their own sample preparation. Still, I like to hear about
other universities where they do things differently.


Thanks,
Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Colleagues,
}
} I have another question regarding TEM work. I want to know how people
} organize TEM work at university laboratories in other parts of the
} World. I have completed my graduate study in United States.
}
} In United States, we were given instructions to operate various sample
} prep tools and TEM itself. And we, as graduate students, were expected
} to prepare our samples and carry out our own TEM study. Which means we
} make few mistakes and break few samples before we could finally
} prepare a meaningful TEM sample.
}
} I want to know how things are done in other parts of the World. Any
} pros and cons when compared to American system?
}
} Thanks a bunch,
} Ayten.
}
}


==============================Original Headers==============================
10, 32 -- From celikaktas-at-gmail.com Mon Apr 6 04:12:53 2009
10, 32 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com [209.85.220.166])
10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n369Cqmd003716
10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 04:12:53 -0500
10, 32 -- Received: by fxm10 with SMTP id 10so1956337fxm.18
10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Apr 2009 02:12:51 -0700 (PDT)
10, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 32 -- d=gmail.com; s=gamma;
10, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject
10, 32 -- :from:to:content-type:content-transfer-encoding;
10, 32 -- bh=hIGF2MYnI1a/nY1D6OydWXjuizPhlwmi5YfHHrdCN7I=;
10, 32 -- b=Gxxh7qNx8lMd9g/K/cqboF511QjbHW0el1ROLR1f3DsYsA2tXiJm/5b87k98Xaudal
10, 32 -- LpX/djODW7NMj1Z803dFehOyBd3rsF7ficPdg29523/5v/rhn3Szd3+2+qIKYzS7vzBv
10, 32 -- OygtC/EsxD/ws3/OjdOIfuqSuEu71LYYz2Bv0=
10, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
10, 32 -- d=gmail.com; s=gamma;
10, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
10, 32 -- :content-transfer-encoding;
10, 32 -- b=mTr7oN5LSIDLqQn2okI58kWsTE0EWUBDv3zMXoH5sxrlnwBBKiykqyQtfk7wwCT2rX
10, 32 -- En2LR8UAJnLQyZ+S/aRPS1VlqX7OA+saZn16IItkHAbACR9rmlJY3nJP4FJ8CeUaoH08
10, 32 -- RxzCSPY+b1CyGiw7Oc6pE9J7JLPr+tPD/IIwc=
10, 32 -- MIME-Version: 1.0
10, 32 -- Received: by 10.103.137.12 with SMTP id p12mr1879669mun.94.1239009171608; Mon,
10, 32 -- 06 Apr 2009 02:12:51 -0700 (PDT)
10, 32 -- Date: Mon, 6 Apr 2009 12:12:51 +0300
10, 32 -- Message-ID: {1075c5c10904060212n63a443f4n701667d38fe5430-at-mail.gmail.com}
10, 32 -- Subject: TEM sample preparation? Re:TEM work at Non-American Universities
10, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
10, 32 -- To: microscopy {Microscopy-at-microscopy.com}
10, 32 -- Content-Type: text/plain; charset=UTF-8
10, 32 -- Content-Transfer-Encoding: 8bit
10, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n369Cqmd003716
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Mon, 6 Apr 2009 04:14:36 -0500
Subject: [Microscopy] Re: viaWWW: Imaging plates for TEM Photo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

William,

I have used Fuji Imaging Plates for electron diffraction study and loved it.

X-from technical point of view, imaging plates have linear response and
higher dynamic range compared to traditional photo films. This is
really important especially for phase retrieval calculations.

Another plus is that one does not need to mess up with chemicals to
develop micrographs.


Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Thu, Apr 2, 2009 at 12:18 AM, {wzhe-at-laurentian.ca} wrote:
}
} Email: wzhe-at-laurentian.ca
} Name: William
}
} Organization: Luarentian Univ
}
} Title-Subject: [Filtered] Imaging plates for TEM Photo
}
} Question: Dear there,
}
} We want to use Fuji imaging plates in TEM photo work and read some
} recommend on product FDL-5000 plate. If someone could comment on this
} imaging plate
}
} 1. What performance looks like in TEM photos?
} 2. Any alternate we can try, because this plate is quite expensive to
} start with, ~US$4000 for a 16 pieces/pack ?
}
} Thanks very much in advance,
}
} William
}
}  Login Host: 142.51.53.72
}


==============================Original Headers==============================
10, 35 -- From celikaktas-at-gmail.com Mon Apr 6 04:14:36 2009
10, 35 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com [209.85.220.166])
10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n369EYEx004923
10, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 04:14:35 -0500
10, 35 -- Received: by fxm10 with SMTP id 10so1957033fxm.18
10, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Apr 2009 02:14:34 -0700 (PDT)
10, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 35 -- d=gmail.com; s=gamma;
10, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
10, 35 -- :date:message-id:subject:from:to:content-type
10, 35 -- :content-transfer-encoding;
10, 35 -- bh=mRXFJXu4y2XmzkSijwN+jIH4ISHmnL5TVN6njMu4GyQ=;
10, 35 -- b=rGDdtUsIu76MAYIRzmbUhMfYCGsFwHTDQygXcE4hHxjA+B3mkoC9B8QHzU2zE0MXPl
10, 35 -- CvDp/RrOtseVgXZtPgAfTfQ592Mdex84AnauvjiyvS9x+E7IZLP3VPZbARrjfDIdzPM3
10, 35 -- YfJ1fidmgPBHoxcOYMLHe9FJTRqD6Ho/TR+rQ=
10, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
10, 35 -- d=gmail.com; s=gamma;
10, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
10, 35 -- :content-type:content-transfer-encoding;
10, 35 -- b=B2JObQHKotZEMsuC2CMfEWFt7qUZ6wAtU8Zj+McZYsvRkqPFr1u+uyzlc+1+kbN8fY
10, 35 -- 5oZFltQSaFjCAfYrGMRnWkOXwWnXSV1cGt9msnujV3WO4vOGC2mH7HWAFql2sQwLXzaX
10, 35 -- hUEclujCKFATQOIAb6K7YNHxLPyMKavbijdio=
10, 35 -- MIME-Version: 1.0
10, 35 -- Received: by 10.103.174.16 with SMTP id b16mr1895811mup.28.1239009274252; Mon,
10, 35 -- 06 Apr 2009 02:14:34 -0700 (PDT)
10, 35 -- In-Reply-To: {200904012118.n31LIfAH028683-at-ns.microscopy.com}
10, 35 -- References: {200904012118.n31LIfAH028683-at-ns.microscopy.com}
10, 35 -- Date: Mon, 6 Apr 2009 12:14:34 +0300
10, 35 -- Message-ID: {1075c5c10904060214p6dfc1f1crc35ac74ead767467-at-mail.gmail.com}
10, 35 -- Subject: Re: [Microscopy] viaWWW: Imaging plates for TEM Photo
10, 35 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
10, 35 -- To: microscopy {Microscopy-at-microscopy.com}
10, 35 -- Content-Type: text/plain; charset=UTF-8
10, 35 -- Content-Transfer-Encoding: 8bit
10, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n369EYEx004923
==============================End of - Headers==============================




From: ben.micklem-at-pharm.ox.ac.uk
Date: Mon, 6 Apr 2009 05:07:24 -0500
Subject: [Microscopy] Re: TEM sample preparation? Re:TEM work at Non-American

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

celikaktas-at-gmail.com wrote:

} Is there any university laboratory (a core facility) out there who has
} a technician to do TEM sample preparation for graduate students?
} Please, let me know if there is such a model out there? Given the
} differences in sample preparation techniques of different kinds of
} samples, I wonder how they do things? Do they have few technicians,
} one for each kind of sample group (metals, biological samples,
} ceramics etc.)?
}
} Personally, I think there are numerous benefits to have graduate
} students do their own sample preparation. Still, I like to hear about
} other universities where they do things differently.

The graduate students here are expected to learn all techniques required
for the project that they undertake, EM is no exception- sometimes it is
impossible for them to always carry out every step of a process (for
example if the facilities to carry out a procedure are not available
here). In these cases every possible attempt is made for them to have
practical experience of carrying out the process once- e.g. a trip to a
collaborating University that is carrying out the procedure.

In terms of EM, the students here have all the facilities they need for
their projects, and they undertake all aspects of their work themselves.
In the past, when we used film for TEM, I would process their film, but
they would make their own prints from the negatives- that was the only
exception. But they all left with a full understanding of how the
development was done.

If EM is only a small side branch of a project, I can see why it is not
so important for the student to spend a lot of time learning all the
stages of preparation, but they should still understand them all, and
preferably carry them out at least once by themselves.

The final stage of doctoral degrees here is the viva voce (similar to
the thesis defence), and the examiners have the right to question the
student on all aspects of the work they have submitted. A thorough
understanding is expected, and having experience in all techniques they
include in their thesis is the best way of achieving this.

Having technicians carry out students' sample preparation is not helping
them gain experience, which is what they are here to do. There are also
not enough technicians to do all the students preparation work, and it
would not be economically sensible if there were.

In our Unit, even post-docs do almost all of their own EM preparation-
they want to be sure that it has been done exactly the same each time,
and up to their own standards. They will spend so much of their own time
examining their grids in the EM, analysing the images and quantifying
the results, that the don't want to risk wasting their time if mistakes
happen- this way, all mistakes must be their own!


Regards,


Ben Micklem

--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

==============================Original Headers==============================
13, 29 -- From ben.micklem-at-pharm.ox.ac.uk Mon Apr 6 05:07:23 2009
13, 29 -- Received: from relay7.mail.ox.ac.uk (relay7.mail.ox.ac.uk [129.67.1.167])
13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n36A7MEs000621
13, 29 -- for {microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 05:07:23 -0500
13, 29 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207])
13, 29 -- by relay7.mail.ox.ac.uk with esmtp (Exim 4.69)
13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
13, 29 -- id 1Lqljc-0003Jj-Ox
13, 29 -- for microscopy-at-microscopy.com; Mon, 06 Apr 2009 11:07:20 +0100
13, 29 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
13, 29 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
13, 29 -- (Exim 4.69)
13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
13, 29 -- id 1Lqljc-0004E4-4k
13, 29 -- for microscopy-at-microscopy.com; Mon, 06 Apr 2009 11:07:20 +0100
13, 29 -- Message-ID: {49D9D458.9090402-at-pharm.ox.ac.uk}
13, 29 -- Date: Mon, 06 Apr 2009 11:07:20 +0100
13, 29 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
13, 29 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
13, 29 -- MIME-Version: 1.0
13, 29 -- To: microscopy-at-microscopy.com
13, 29 -- Subject: Re: [Microscopy] TEM sample preparation? Re:TEM work at Non-American
13, 29 -- Universities
13, 29 -- References: {200904060931.n369VLN8030462-at-ns.microscopy.com}
13, 29 -- In-Reply-To: {200904060931.n369VLN8030462-at-ns.microscopy.com}
13, 29 -- X-Enigmail-Version: 0.95.7
13, 29 -- Content-Type: text/plain; charset=ISO-8859-1
13, 29 -- Content-Transfer-Encoding: 7bit
13, 29 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================




From: lherault-at-bu.edu
Date: Mon, 6 Apr 2009 08:27:39 -0500
Subject: [Microscopy] TEM sample preparation? Re:TEM work at Non-American

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't have a TEM here and admittedly, most of our SEM preps are pretty
straightforward mounts of solid, never-alive material (e.g. ceramics) but I
like to show our students how to do the preparation work and urge them to
develop a good understanding of the whys. Then they have to do their own
specimen mounting and preparation. They will leave hear and may end up
doing further research and/or teaching others so they should have a working
knowledge of how things are done, if for no other reason than to understand
what they read about other people's research and to recognize if it has been
done well or poorly.

Ron L

-----Original Message-----
X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com]
Sent: Monday, April 06, 2009 5:20 AM
To: lherault-at-bu.edu

Thanks to all who have responded to my previous inquiries.

Some university laboratories have one or two designated TEM operators
who operate TEM for everyone in some parts of the world. Commercial
TEM laboratories generally have specific areas of expertise.

Is there any university laboratory (a core facility) out there who has
a technician to do TEM sample preparation for graduate students?
Please, let me know if there is such a model out there? Given the
differences in sample preparation techniques of different kinds of
samples, I wonder how they do things? Do they have few technicians,
one for each kind of sample group (metals, biological samples,
ceramics etc.)?

Personally, I think there are numerous benefits to have graduate
students do their own sample preparation. Still, I like to hear about
other universities where they do things differently.


Thanks,
Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote:
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of
America
} To  Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Dear Colleagues,
}
} I have another question regarding TEM work. I want to know how people
} organize TEM work at university laboratories in other parts of the
} World. I have completed my graduate study in United States.
}
} In United States, we were given instructions to operate various sample
} prep tools and TEM itself. And we, as graduate students, were expected
} to prepare our samples and carry out our own TEM study. Which means we
} make few mistakes and break few samples before we could finally
} prepare a meaningful TEM sample.
}
} I want to know how things are done in other parts of the World. Any
} pros and cons when compared to American system?
}
} Thanks a bunch,
} Ayten.
}
}


==============================Original Headers==============================
10, 32 -- From celikaktas-at-gmail.com Mon Apr 6 04:12:53 2009
10, 32 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com
[209.85.220.166])
10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n369Cqmd003716
10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 04:12:53
-0500
10, 32 -- Received: by fxm10 with SMTP id 10so1956337fxm.18
10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Apr 2009 02:12:51
-0700 (PDT)
10, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 32 -- d=gmail.com; s=gamma;
10, 32 --
h=domainkey-signature:mime-version:received:date:message-id:subject
10, 32 -- :from:to:content-type:content-transfer-encoding;
10, 32 -- bh=hIGF2MYnI1a/nY1D6OydWXjuizPhlwmi5YfHHrdCN7I=;
10, 32 --
b=Gxxh7qNx8lMd9g/K/cqboF511QjbHW0el1ROLR1f3DsYsA2tXiJm/5b87k98Xaudal
10, 32 --
LpX/djODW7NMj1Z803dFehOyBd3rsF7ficPdg29523/5v/rhn3Szd3+2+qIKYzS7vzBv
10, 32 -- OygtC/EsxD/ws3/OjdOIfuqSuEu71LYYz2Bv0=
10, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
10, 32 -- d=gmail.com; s=gamma;
10, 32 --
h=mime-version:date:message-id:subject:from:to:content-type
10, 32 -- :content-transfer-encoding;
10, 32 --
b=mTr7oN5LSIDLqQn2okI58kWsTE0EWUBDv3zMXoH5sxrlnwBBKiykqyQtfk7wwCT2rX
10, 32 --
En2LR8UAJnLQyZ+S/aRPS1VlqX7OA+saZn16IItkHAbACR9rmlJY3nJP4FJ8CeUaoH08
10, 32 -- RxzCSPY+b1CyGiw7Oc6pE9J7JLPr+tPD/IIwc=
10, 32 -- MIME-Version: 1.0
10, 32 -- Received: by 10.103.137.12 with SMTP id
p12mr1879669mun.94.1239009171608; Mon,
10, 32 -- 06 Apr 2009 02:12:51 -0700 (PDT)
10, 32 -- Date: Mon, 6 Apr 2009 12:12:51 +0300
10, 32 -- Message-ID:
{1075c5c10904060212n63a443f4n701667d38fe5430-at-mail.gmail.com}
10, 32 -- Subject: TEM sample preparation? Re:TEM work at Non-American
Universities
10, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
10, 32 -- To: microscopy {Microscopy-at-microscopy.com}
10, 32 -- Content-Type: text/plain; charset=UTF-8
10, 32 -- Content-Transfer-Encoding: 8bit
10, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n369Cqmd003716
==============================End of - Headers==============================



==============================Original Headers==============================
19, 23 -- From lherault-at-bu.edu Mon Apr 6 08:27:39 2009
19, 23 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62])
19, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n36DRbkb023515
19, 23 -- for {microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 08:27:38 -0500
19, 23 -- X-Envelope-From: lherault-at-bu.edu
19, 23 -- Received: from BUSDM801203 ([155.41.214.120])
19, 23 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n36DQkSM013793
19, 23 -- for {microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 09:26:47 -0400
19, 23 -- From: "Ron L" {lherault-at-bu.edu}
19, 23 -- To: {microscopy-at-microscopy.com}
19, 23 -- References: {200904060919.n369Jmnc012965-at-ns.microscopy.com}
19, 23 -- Subject: RE: [Microscopy] TEM sample preparation? Re:TEM work at Non-American Universities
19, 23 -- Date: Mon, 6 Apr 2009 09:26:46 -0400
19, 23 -- Message-ID: {002701c9b6bb$55f35600$78d6299b-at-ad.bu.edu}
19, 23 -- MIME-Version: 1.0
19, 23 -- Content-Type: text/plain;
19, 23 -- charset="iso-8859-1"
19, 23 -- X-Mailer: Microsoft Office Outlook 11
19, 23 -- In-Reply-To: {200904060919.n369Jmnc012965-at-ns.microscopy.com}
19, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
19, 23 -- Thread-Index: Acm2mNrdP4EoANUpROGZ1f2H7E/f2gAH9kSw
19, 23 -- Content-Transfer-Encoding: 8bit
19, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n36DRbkb023515
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Mon, 6 Apr 2009 17:18:30 -0500
Subject: [Microscopy] Re: TEM sample preparation? Re:TEM work at Non-American

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ayten,

In the microscopy centre here, while we have a couple of SEMs, a confocal,
several fluorescence microscopes, microtomes, etc., it's just me and my
technical officer (who also has a cell biology-oriented PhD). Students and
postdocs should learn to do their own preparations - how will they know what
to do/how to advise their students when they start their own labs? And what
if they are hired partly on their cell biology/microscopy skills as seen in
a publication?

After all, you don't have a centre where everyone goes to get their gels or
PCRs run by someone else, or to get their constructs made, do you (or maybe
you do - not here, anyway)? Here, the lab techs also have to learn
microscopy from go to whoa, and unless it's a collaboration, all the
permanent scientists do, too. It's good for them.... they finally realise
how much work and experience and TIME!! goes in to "taking that picture" -
and they learn something new, too.

It wasn't like that when I arrived - people were used to handing material in
at the door and getting an image out, and while was some grumbling at first,
the switch has meant the instruments get used more - seemed crazy that there
was so much equipment used about 5% of the time - now it's more like 50-70%,
with some, like the confocal, used all day most days.

So, even in a non-university lab, which we are, we still do things this way.

cheers,
Rosemary


Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 6/04/09 8:26 PM, "celikaktas-at-gmail.com" {celikaktas-at-gmail.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Thanks to all who have responded to my previous inquiries.
}
} Some university laboratories have one or two designated TEM operators
} who operate TEM for everyone in some parts of the world. Commercial
} TEM laboratories generally have specific areas of expertise.
}
} Is there any university laboratory (a core facility) out there who has
} a technician to do TEM sample preparation for graduate students?
} Please, let me know if there is such a model out there? Given the
} differences in sample preparation techniques of different kinds of
} samples, I wonder how they do things? Do they have few technicians,
} one for each kind of sample group (metals, biological samples,
} ceramics etc.)?
}
} Personally, I think there are numerous benefits to have graduate
} students do their own sample preparation. Still, I like to hear about
} other universities where they do things differently.
}
}
} Thanks,
} Ayten.
}
} --
} ===========================
} Ayten Celik-Aktas, PhD
} Ankara University
} Electron Microscopy Laboratory
} Ankara, Turkey
} ===========================
}
}
} On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear Colleagues,
} }
} } I have another question regarding TEM work. I want to know how people
} } organize TEM work at university laboratories in other parts of the
} } World. I have completed my graduate study in United States.
} }
} } In United States, we were given instructions to operate various sample
} } prep tools and TEM itself. And we, as graduate students, were expected
} } to prepare our samples and carry out our own TEM study. Which means we
} } make few mistakes and break few samples before we could finally
} } prepare a meaningful TEM sample.
} }
} } I want to know how things are done in other parts of the World. Any
} } pros and cons when compared to American system?
} }
} } Thanks a bunch,
} } Ayten.
} }
} }
}
}
} ==============================Original Headers==============================
} 10, 32 -- From celikaktas-at-gmail.com Mon Apr 6 04:12:53 2009
} 10, 32 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com
} [209.85.220.166])
} 10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n369Cqmd003716
} 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 04:12:53 -0500
} 10, 32 -- Received: by fxm10 with SMTP id 10so1956337fxm.18
} 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Apr 2009 02:12:51
} -0700 (PDT)
} 10, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 10, 32 -- d=gmail.com; s=gamma;
} 10, 32 --
} h=domainkey-signature:mime-version:received:date:message-id:subject
} 10, 32 -- :from:to:content-type:content-transfer-encoding;
} 10, 32 -- bh=hIGF2MYnI1a/nY1D6OydWXjuizPhlwmi5YfHHrdCN7I=;
} 10, 32 --
} b=Gxxh7qNx8lMd9g/K/cqboF511QjbHW0el1ROLR1f3DsYsA2tXiJm/5b87k98Xaudal
} 10, 32 --
} LpX/djODW7NMj1Z803dFehOyBd3rsF7ficPdg29523/5v/rhn3Szd3+2+qIKYzS7vzBv
} 10, 32 -- OygtC/EsxD/ws3/OjdOIfuqSuEu71LYYz2Bv0=
} 10, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 10, 32 -- d=gmail.com; s=gamma;
} 10, 32 -- h=mime-version:date:message-id:subject:from:to:content-type
} 10, 32 -- :content-transfer-encoding;
} 10, 32 --
} b=mTr7oN5LSIDLqQn2okI58kWsTE0EWUBDv3zMXoH5sxrlnwBBKiykqyQtfk7wwCT2rX
} 10, 32 --
} En2LR8UAJnLQyZ+S/aRPS1VlqX7OA+saZn16IItkHAbACR9rmlJY3nJP4FJ8CeUaoH08
} 10, 32 -- RxzCSPY+b1CyGiw7Oc6pE9J7JLPr+tPD/IIwc=
} 10, 32 -- MIME-Version: 1.0
} 10, 32 -- Received: by 10.103.137.12 with SMTP id
} p12mr1879669mun.94.1239009171608; Mon,
} 10, 32 -- 06 Apr 2009 02:12:51 -0700 (PDT)
} 10, 32 -- Date: Mon, 6 Apr 2009 12:12:51 +0300
} 10, 32 -- Message-ID:
} {1075c5c10904060212n63a443f4n701667d38fe5430-at-mail.gmail.com}
} 10, 32 -- Subject: TEM sample preparation? Re:TEM work at Non-American
} Universities
} 10, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
} 10, 32 -- To: microscopy {Microscopy-at-microscopy.com}
} 10, 32 -- Content-Type: text/plain; charset=UTF-8
} 10, 32 -- Content-Transfer-Encoding: 8bit
} 10, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n369Cqmd003716
} ==============================End of - Headers==============================




==============================Original Headers==============================
15, 48 -- From prvs=34043260e=Rosemary.White-at-csiro.au Mon Apr 6 17:18:29 2009
15, 48 -- Received: from act-MTAout4.csiro.au (act-MTAout4.csiro.au [150.229.7.41])
15, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n36MIR8H027809
15, 48 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 17:18:28 -0500
15, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
15, 48 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
15, 48 -- s=email; t=1239056308; x=1270592308;
15, 48 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
15, 48 -- mime-version:content-transfer-encoding:content-id:
15, 48 -- content-description:resent-date:resent-from:resent-sender:
15, 48 -- resent-to:resent-cc:resent-message-id:in-reply-to:
15, 48 -- references:list-id:list-help:list-unsubscribe:
15, 48 -- list-subscribe:list-post:list-owner:list-archive;
15, 48 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
15, 48 -- |Subject:=20Re:=20[Microscopy]=20TEM=20sample=20preparati
15, 48 -- on?=20Re:TEM=20work=20at=20Non-American=0D=0A=20Universit
15, 48 -- ies|Date:=20Tue,=207=20Apr=202009=2008:18:20=20+1100
15, 48 -- |Message-ID:=20 {C600BCCC.4DF0%rosemary.white-at-csiro.au}
15, 48 -- |To:=20 {Microscopy-at-microscopy.com} |MIME-Version:=201.0
15, 48 -- |Content-Transfer-Encoding:=20quoted-printable
15, 48 -- |In-Reply-To:=20 {200904060926.n369QGVr022016-at-ns.microscop
15, 48 -- y.com} ;
15, 48 -- bh=QoWOY74UQk4ubxXjyvv+3fVpDmcRdyDY/Bfd3F2s6rg=;
15, 48 -- b=QMR03/8WihrOnGXl6Ht83nxG50jmP5WwWRCNmTz2mbYiMxVJdd/TqK4Y
15, 48 -- xNgb0ZEjak5fbrc6D1rS2LkdmGaJ+86zw9cFQm0crepefDWMMmukJrLrb
15, 48 -- 0osD+O0ifOrJUoe;
15, 48 -- X-IronPort-AV: E=Sophos;i="4.39,332,1235912400";
15, 48 -- d="scan'208";a="24311646"
15, 48 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
15, 48 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 07 Apr 2009 08:18:25 +1000
15, 48 -- Received: from [152.83.193.49] (152.83.193.49) by EXNSW-HTCA01.nexus.csiro.au
15, 48 -- (130.155.117.126) with Microsoft SMTP Server id 8.1.340.0; Tue, 7 Apr 2009
15, 48 -- 08:18:25 +1000
15, 48 -- User-Agent: Microsoft-Entourage/12.10.0.080409
15, 48 -- Date: Tue, 7 Apr 2009 08:18:20 +1100
15, 48 -- Subject: Re: [Microscopy] TEM sample preparation? Re:TEM work at Non-American
15, 48 -- Universities
15, 48 -- From: Rosemary White {rosemary.white-at-csiro.au}
15, 48 -- To: {Microscopy-at-microscopy.com}
15, 48 -- Message-ID: {C600BCCC.4DF0%rosemary.white-at-csiro.au}
15, 48 -- Thread-Topic: [Microscopy] TEM sample preparation? Re:TEM work at Non-American
15, 48 -- Universities
15, 48 -- Thread-Index: Acm2mcFW2xWRCVT/SC2O3AMMnaPnFgAa9YVl
15, 48 -- In-Reply-To: {200904060926.n369QGVr022016-at-ns.microscopy.com}
15, 48 -- MIME-Version: 1.0
15, 48 -- Content-Type: text/plain; charset="ISO-8859-1"
15, 48 -- Content-Transfer-Encoding: 8bit
15, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n36MIR8H027809
==============================End of - Headers==============================




From: ahmad_ds-at-yahoo.com
Date: Tue, 7 Apr 2009 07:58:32 -0500
Subject: [Microscopy] viaWWW: Oval TEM Beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkhaibi

Organization: BAU

Title-Subject: [Filtered] Oval TEM Beam

Question: I've got an oval beam as I started my TEM...and it get's a
line shape as I increase the intensity what seems to be the problem?
I've tried to adjust the astigmatism, but didn't work out.

Thanks.

Login Host: 87.236.233.99
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Apr 7 07:58:32 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37CwVm2015237
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 07:58:32 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c600fe678b5b-at-[206.69.208.22]}
7, 11 -- Date: Tue, 7 Apr 2009 07:58:30 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Oval TEM Beam
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Tue, 7 Apr 2009 09:24:42 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Senior Scientist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You haven't given much detail so it could be several things.

For instance have you just changed the filament recently or done any
other work in the electron gun or condenser area? What happens if you
adjust focus of the condenser lens from underfocus to overfocus - does
the direction of the oval shape change? Have you tried checking the
complete alignment of the condenser system eg gun tilt, gun shift,
movable condenser alignment? Finally I apologise for asking but you
say you have adjusted the astigmatism - I assume you mean the
condenser astigmatism?

If this has happened after a filament change then it could be a badly
positioned filament or defective one. It could even be movement of the
gun or condenser aperture.

Other possibilities might include some form of wobbler or scan system
inadvertently switched on.

It might be useful to know what TEM you are using, as well.

Good luck

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: ahmad_ds-at-yahoo.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David Parmiter

Organization: SAIC-Frederick

Title-Subject: [Filtered] Senior Scientist Position

Question: To all -

We are looking for a Senior Scientist to run the
Electron Microscopy Facility at SAIC-Frederick, a
contractor for the National Cancer Institute.
For all applications or inquiries, please contact
the individual mentioned at the bottom. Thanks!

Position description and requirements:

SAIC-Frederick, Inc., a subsidiary of SAIC,
develops and applies advanced technologies to
meet the most urgent and challenging research and
development needs of the National Cancer
Institute, other government agencies, and the
nation. We are the prime contractor for the
National Cancer Institute at Frederick, one of 38
Federally Funded Research and Development Centers
and the only such national laboratory devoted
exclusively to biomedical research.

We are currently recruiting for a Senior
Scientist (128940) at SAIC-Frederick. This
scientist will manage the staff and operations of
the electron microscopy (EM) laboratory at
SAIC-Frederick. Specific duties include (i)
managerial oversight of the EM laboratoryís core
services (ii) interface with intra- and
extramural investigators to provide EM
characterization support (iii) methods
development and research using techniques such as
energy dispersive x-ray spectroscopy (EDS),
cryogenics, focused ion beam ablation, 3D
tomography on biological samples and (iv)
interpretation of results and preparation of
written reports. He/she will also work with an
interdisciplinary team of scientists to
characterize nanomaterials intended for cancer
therapeutics and diagnostics.

Possession of a doctoral degree from an
accredited college/ university in a field related
to Chemistry, Biology, Material Science,
Engineering or Physics. Foreign educated
candidates who have completed part or all of
their education outside of the United States must
have their foreign education evaluated by an
SAIC-approved accrediting organization to assure
that it has met the equivalency of the
qualifications of degree work in the United
States. In addition to the educational
requirements, a minimum of five years of related
experience. A minimum of three years using
electron microscopy (TEM, SEM) on biological
tissues. Experience with energy dispersive x-ray
spectroscopy (EDS), cryogenics, focused ion beam
ablation, and 3D tomography. This position is
subject to obtaining a Public Trust Clearance.

Excellent compensation package accompanies our
position. For immediate consideration, please
apply online at our website: www.saic.com for
position #128940. SAIC is an equal opportunity
employer and values cultural diversity in the
workplace.

Nelmarie Miranda (Contractor)
Senior Employment Specialist
SAIC-Frederick, Inc.
National Cancer Institute at Frederick
Post Office Box B
Frederick, MD 21702
mirandan-at-mail.nih.gov


Login Host: 129.43.43.217
---------------------------------------------------------------------------


==============================Original Headers==============================
15, 13 -- From zaluzec-at-microscopy.com Tue Apr 7 09:24:41 2009
15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37EOfv1014218
15, 13 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 09:24:41 -0500
15, 13 -- Mime-Version: 1.0
15, 13 -- Message-Id: {p06240800c60112857a1f-at-[206.69.208.22]}
15, 13 -- Date: Tue, 7 Apr 2009 09:24:40 -0500
15, 13 -- To: microscopy-at-microscopy.com
15, 13 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
15, 13 -- Subject: [Filtered] MicroscopyListserverviaWWW: Senior Scientist Position
15, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
15, 13 -- Content-Transfer-Encoding: 8bit
15, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37EOfv1014218
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Tue, 7 Apr 2009 09:38:38 -0500
Subject: [Microscopy] viaWWW: Oval TEM Beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

What you are describing is known as Condenser Astigmatism. On your
microscope there will be at least two devices for correcting the
astigmatism, one for the condenser system, one for the objective system and
possibly one for the intermediate system.

I feel that when you say you have tried adjusting the astigmatism you have
used the objective controls not the condenser.

Try the following

1. With the beam on adjust the second condenser (illumination or
brightness on some instruments) to cross over, the smallest beam spot.
2. Increase the magnification to make the spot about 2 to 3cms across.
3. Decrease the filament heating until the beam breaks up into a spot
and halo formation.
4. Adjust the illumination to focus this image as sharp as you can
5. Adjust each condenser stigmator in turn until the spot and halo
image is at its sharpest.
6. Repeat 4 and 5 until you have no improvement.
7. Heat the filament to the level you require for your tasks.

Good luck

Steve

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com
-----Original Message-----
X-from: ahmad_ds-at-yahoo.com [mailto:ahmad_ds-at-yahoo.com]
Sent: 07 April 2009 14:02
To: protrain-at-emcourses.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkhaibi

Organization: BAU

Title-Subject: [Filtered] Oval TEM Beam

Question: I've got an oval beam as I started my TEM...and it get's a
line shape as I increase the intensity what seems to be the problem?
I've tried to adjust the astigmatism, but didn't work out.

Thanks.

Login Host: 87.236.233.99
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Apr 7 07:58:32 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n37CwVm2015237
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 07:58:32
-0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c600fe678b5b-at-[206.69.208.22]}
7, 11 -- Date: Tue, 7 Apr 2009 07:58:30 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Oval TEM Beam
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 27 -- From protrain-at-emcourses.com Tue Apr 7 09:38:37 2009
20, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37EcaZ1028685
20, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 09:38:37 -0500
20, 27 -- Received: from 5ac8bf86.bb.sky.com ([90.200.191.134]:2696 helo=HP6220)
20, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
20, 27 -- (envelope-from {protrain-at-emcourses.com} )
20, 27 -- id 1LrCRa-000KRj-Vv by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Tue, 07 Apr 2009 16:38:32 +0200
20, 27 -- Reply-To: {protrain-at-emcourses.com}
20, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com}
20, 27 -- To: {ahmad_ds-at-yahoo.com}
20, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
20, 27 -- References: {200904071301.n37D1WaT016729-at-ns.microscopy.com}
20, 27 -- Subject: RE: [Microscopy] viaWWW: Oval TEM Beam
20, 27 -- Date: Tue, 7 Apr 2009 15:38:05 +0100
20, 27 -- Organization: Protrain
20, 27 -- Message-ID: {001b01c9b78e$85fd0340$0200a8c0-at-HP6220}
20, 27 -- MIME-Version: 1.0
20, 27 -- Content-Type: text/plain;
20, 27 -- charset="us-ascii"
20, 27 -- Content-Transfer-Encoding: 7bit
20, 27 -- X-Mailer: Microsoft Office Outlook 11
20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
20, 27 -- Thread-Index: Acm3gQwxagHdYSe2RemQ6KiA3yNFlgADEkog
20, 27 -- In-Reply-To: {200904071301.n37D1WaT016729-at-ns.microscopy.com}
20, 27 -- X-Scan-Signature: 1de276dc42294cb40301ecefa06be1b0{86}}
20, 27 -- X-Trace: smtp01.dial-up.net 1LrCRa-000KRj-Vv 44a47c05ff5e35e153711f610de77e3e
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Tue, 7 Apr 2009 10:29:51 -0500
Subject: [Microscopy] storing slides before immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
How long can slides with sections be stored before they are used for
immunolabeling? 6 months in the refrigerator? or longer? or is it a
bad idea to wait?
I usually cut sections then label the next day but someone here would
like to store the slides for awhile if that is an okay practice.

Any advice would be greatly appreciated.
thanks,
Beth



**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com





==============================Original Headers==============================
14, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 10:29:51 2009
14, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FToR8012316
14, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:29:50 -0500
14, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
14, 19 -- (authenticated user beth-at-plantbio.uga.edu)
14, 19 -- by dogwood.plantbio.uga.edu
14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
14, 19 -- for microscopy-at-microscopy.com;
14, 19 -- Tue, 7 Apr 2009 11:29:46 -0400
14, 19 -- Message-Id: {13CA0374-0445-42E7-8DBB-62B77A025359-at-plantbio.uga.edu}
14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
14, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
14, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
14, 19 -- Content-Transfer-Encoding: 7bit
14, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
14, 19 -- Subject: storing slides before immunolabeling
14, 19 -- Date: Tue, 7 Apr 2009 11:29:21 -0400
14, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 7 Apr 2009 10:35:22 -0500
Subject: [Microscopy] RE: FFT with digital TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks a lot for numerous answers. They were really helpful.
Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
} Sent: Friday, April 03, 2009 4:27 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] FFT with digital TEM camera
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi,
}
} I have a question about TEM digital cameras.
} Are they capable of displaying live FFT images for stigmation
} and focusing? Do this capability come usually as option or
} built in basic package?
}
} Thank you,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} ==============================Original
} Headers==============================
} 8, 23 -- From DusevichV-at-umkc.edu Fri Apr 3 16:26:00 2009 8,
} 23 -- Received: from KC-MSXPROTO2.kc.umkc.edu
} (smtp.exchange.umkc.edu [134.193.143.155])
} 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n33LQ0M5005264
} 8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Apr
} 2009 16:26:00 -0500
} 8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11])
} by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 23 -- Fri, 3 Apr 2009 16:25:59 -0500
} 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23
} -- Content-class: urn:content-classes:message 8, 23 --
} MIME-Version: 1.0 8, 23 -- Content-Type: text/plain;
} 8, 23 -- charset="us-ascii"
} 8, 23 -- Subject: FFT with digital TEM camera 8, 23 -- Date:
} Fri, 3 Apr 2009 16:25:58 -0500 8, 23 -- Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB813-at-KC-MSX1.kc.umkc.edu}
} 8, 23 -- X-MS-Has-Attach:
} 8, 23 -- X-MS-TNEF-Correlator:
} 8, 23 -- Thread-Topic: FFT with digital TEM camera 8, 23 --
} Thread-Index: Acm0osfdDP1JWk8gQlGXKZa4ieaK0g== 8, 23 -- From:
} "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 23 -- To:
} {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime:
} 03 Apr 2009 21:25:59.0306 (UTC) FILETIME=[C82FFEA0:01C9B4A2]
} 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 --
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n33LQ0M5005264
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
6, 25 -- From DusevichV-at-umkc.edu Tue Apr 7 10:35:22 2009
6, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FZL0q017972
6, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:35:21 -0500
6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Tue, 7 Apr 2009 10:35:20 -0500
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="us-ascii"
6, 25 -- Subject: RE: [Microscopy] FFT with digital TEM camera
6, 25 -- Date: Tue, 7 Apr 2009 10:35:19 -0500
6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB816-at-KC-MSX1.kc.umkc.edu}
6, 25 -- In-Reply-To: {200904032126.n33LQeOj006145-at-ns.microscopy.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: [Microscopy] FFT with digital TEM camera
6, 25 -- Thread-Index: Acm0ouLhQxEk3ZNaSF6fPMLW5oqD4AC72Jpg
6, 25 -- References: {200904032126.n33LQeOj006145-at-ns.microscopy.com}
6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 07 Apr 2009 15:35:20.0171 (UTC) FILETIME=[7589C3B0:01C9B796]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37FZL0q017972
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Tue, 7 Apr 2009 10:48:28 -0500
Subject: [Microscopy] Manuals for S-570 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers
I have a complete set of manuals (installation, service and user) for a
Hitachi S-570 SEM available free - if this is of interest to you please
contact me off list

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca





==============================Original Headers==============================
7, 26 -- From rjharris-at-uwo.ca Tue Apr 7 10:48:28 2009
7, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FmRld007967
7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:48:28 -0500
7, 26 -- MIME-version: 1.0
7, 26 -- Content-type: text/plain; charset=iso-8859-1
7, 26 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41])
7, 26 -- by zeppo.mail.uwo.pri
7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 26 -- with ESMTP id {0KHQ0085WMKPTYI0-at-zeppo.mail.uwo.pri} for
7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT)
7, 26 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
7, 26 -- by zeppo.mail.uwo.pri
7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
7, 26 -- with ESMTPSA id {0KHQ00MXHMKPR650-at-zeppo.mail.uwo.pri} for
7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT)
7, 26 -- From: Richard Harris {rjharris-at-uwo.ca}
7, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com}
7, 26 -- Subject: Manuals for S-570 SEM
7, 26 -- Date: Tue, 07 Apr 2009 11:48:26 -0400
7, 26 -- Message-id: {002601c9b798$4a45f320$ded1d960$-at-ca}
7, 26 -- X-Mailer: Microsoft Office Outlook 12.0
7, 26 -- Content-language: en-us
7, 26 -- Thread-index: Acm3mEoiKth8qgRsTZSHCx9a+yeIWw==
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37FmRld007967
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Tue, 7 Apr 2009 11:02:11 -0500
Subject: [Microscopy] storing slides - section info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PS - I forgot to say that the tissue is embedded in LR White.

**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com





==============================Original Headers==============================
11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009
11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252
11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500
11, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
11, 19 -- (authenticated user beth-at-plantbio.uga.edu)
11, 19 -- by dogwood.plantbio.uga.edu
11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
11, 19 -- for microscopy-at-microscopy.com;
11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400
11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu}
11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 19 -- Subject: storing slides - section info
11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400
11, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 7 Apr 2009 11:09:39 -0500
Subject: [Microscopy] SEM: Locating bacteria in fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This comes under "Looking for small things in large places".

One of our clients has determined the presence, but not the location, of
bacteria in mushroom fruiting bodies. The mushrooms are about the size
of the common white mushrooms you buy at the grocery store. He wants to
know where the bugs be.

We have tried breaking pieces off the mushroom and viewing them in an
environmental SEM and, while we found all sorts of neat reproductive
stuff, no bacteria were found. To search systematically through an
entire fruiting body with no clue as to where the wee beasties might be
can be done, but it's going to be expensive and time-consuming,
especially since we don't know their physical appearance.

TEM seems completely impractical for this for obvious reasons (if not,
please enlighten me).

So, if anyone has ideas on looking for needles in a fungal haystack, I'm
all ears. Real ears, not wood ears.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original Headers==============================
9, 27 -- From TindallR-at-missouri.edu Tue Apr 7 11:09:39 2009
9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G9cl7000497
9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:09:39 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEALkX20nRauUo/2dsb2JhbAC/VAEJhwGITYJKgTMG
9, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
9, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 11:09:36 -0500
9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 27 -- Tue, 7 Apr 2009 11:09:35 -0500
9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 27 -- Content-class: urn:content-classes:message
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Type: text/plain;
9, 27 -- charset="us-ascii"
9, 27 -- Subject: SEM: Locating bacteria in fungi
9, 27 -- Date: Tue, 7 Apr 2009 11:09:35 -0500
9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FC9-at-UM-XMAIL08.um.umsystem.edu}
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- Thread-Topic: SEM: Locating bacteria in fungi
9, 27 -- Thread-Index: Acm3mz5qeov9y91NQHG6WZ82emeIxA==
9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 27 -- To: {microscopy-at-microscopy.com}
9, 27 -- X-OriginalArrivalTime: 07 Apr 2009 16:09:35.0394 (UTC) FILETIME=[3E8BE820:01C9B79B]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37G9cl7000497
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Tue, 7 Apr 2009 11:47:45 -0500
Subject: [Microscopy] storing slides before immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What type of sections are you talking about? Resin, paraffin, or cryo? I
store resin and paraffin for years with no apparent problem. I have
stored cryo for months at -80 but you risk desiccation or other
problems. Usually it is obvious when the cryo morphology has
deteriorated. Good luck.

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Tuesday, April 07, 2009 10:31 AM
To: Phillips, Thomas E.

Hi all,
How long can slides with sections be stored before they are used for
immunolabeling? 6 months in the refrigerator? or longer? or is it a
bad idea to wait?
I usually cut sections then label the next day but someone here would
like to store the slides for awhile if that is an okay practice.

Any advice would be greatly appreciated.
thanks,
Beth



**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com





==============================Original
Headers==============================
14, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 10:29:51 2009
14, 19 -- Received: from dogwood.plantbio.uga.edu
(dogwood.plantbio.uga.edu [128.192.26.2])
14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n37FToR8012316
14, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009
10:29:50 -0500
14, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
14, 19 -- (authenticated user beth-at-plantbio.uga.edu)
14, 19 -- by dogwood.plantbio.uga.edu
14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
14, 19 -- for microscopy-at-microscopy.com;
14, 19 -- Tue, 7 Apr 2009 11:29:46 -0400
14, 19 -- Message-Id:
{13CA0374-0445-42E7-8DBB-62B77A025359-at-plantbio.uga.edu}
14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
14, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
14, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
14, 19 -- Content-Transfer-Encoding: 7bit
14, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
14, 19 -- Subject: storing slides before immunolabeling
14, 19 -- Date: Tue, 7 Apr 2009 11:29:21 -0400
14, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 29 -- From PhillipsT-at-missouri.edu Tue Apr 7 11:47:45 2009
24, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37GliQU019253
24, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:47:44 -0500
24, 29 -- X-IronPort-Anti-Spam-Filtered: true
24, 29 -- X-IronPort-Anti-Spam-Result: ApoEACcg20nRauUo/2dsb2JhbAC/XgEJhwyITQGCSYEzBoc/
24, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
24, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 11:47:44 -0500
24, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
24, 29 -- Tue, 7 Apr 2009 11:47:43 -0500
24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 29 -- Content-class: urn:content-classes:message
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Type: text/plain;
24, 29 -- charset="us-ascii"
24, 29 -- Subject: RE: [Microscopy] storing slides before immunolabeling
24, 29 -- Date: Tue, 7 Apr 2009 11:47:41 -0500
24, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD064EA3E2-at-UM-XMAIL06.um.umsystem.edu}
24, 29 -- In-Reply-To: {200904071531.n37FVEw3013445-at-ns.microscopy.com}
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- Thread-Topic: [Microscopy] storing slides before immunolabeling
24, 29 -- Thread-Index: Acm3leW6d1zZSsI0QJOluEAPq3fKmgAChohA
24, 29 -- References: {200904071531.n37FVEw3013445-at-ns.microscopy.com}
24, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
24, 29 -- To: {beth-at-plantbio.uga.edu} , {Microscopy-at-microscopy.com}
24, 29 -- X-OriginalArrivalTime: 07 Apr 2009 16:47:43.0908 (UTC) FILETIME=[929B6E40:01C9B7A0]
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37GliQU019253
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Tue, 7 Apr 2009 12:29:45 -0500
Subject: [Microscopy] Re: storing slides - section info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tissue should be relatively stable in epoxy even at room temperature.
Consider Kristina Micheva's (Stephen Smith Lab) Array Tomography
technique where LR White sections are repeatedly immunolabeled and each
label is eluted with .2M NaOH and .1% SDS before the next label is
applied. Of course, there is always an antigen that is the exception.
Larry

beth-at-plantbio.uga.edu wrote:
}
Hi all,
How long can slides with sections be stored before they are used for
immunolabeling? 6 months in the refrigerator? or longer? or is it a
bad idea to wait?
I usually cut sections then label the next day but someone here would
like to store the slides for awhile if that is an okay practice.

Any advice would be greatly appreciated.
thanks,
Beth
}
} PS - I forgot to say that the tissue is embedded in LR White.
}
} **********************************************************************
} Beth Richardson
} Electron Microscopy Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} http://www.plantbio.uga.edu/emlab/
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} *******************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************
} The Friends of the Marine Institute - Join Today!
} www.friendsofugami.com
}
}
}
}
}
} ==============================Original Headers==============================
} 11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009
} 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
} 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252
} 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500
} 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
} 11, 19 -- (authenticated user beth-at-plantbio.uga.edu)
} 11, 19 -- by dogwood.plantbio.uga.edu
} 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
} 11, 19 -- for microscopy-at-microscopy.com;
} 11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400
} 11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu}
} 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
} 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
} 11, 19 -- Content-Transfer-Encoding: 7bit
} 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 11, 19 -- Subject: storing slides - section info
} 11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400
} 11, 19 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
7, 39 -- From Larry.Ackerman-at-ucsf.edu Tue Apr 7 12:29:39 2009
7, 39 -- Received: from emfmcb01.ucsfmedicalcenter.org (EMFMCB01.ucsfmedicalcenter.org [64.54.46.97])
7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37HTajp002078
7, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 12:29:37 -0500
7, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with
7, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
7, 39 -- Tue, 07 Apr 2009 10:28:34 -0700
7, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
7, 39 -- X-AuditID: 403623d2-a7709bb0000017ee-a5-49db8d42a758
7, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org (
7, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by
7, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
7, 39 -- 63FC115BD for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:28:34
7, 39 -- -0700 (PDT)
7, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
7, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.3959);
7, 39 -- Tue, 7 Apr 2009 10:28:34 -0700
7, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
7, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Tue, 7 Apr
7, 39 -- 2009 10:28:33 -0700
7, 39 -- Message-ID: {49DB8D3B.60402-at-ucsf.edu}
7, 39 -- Date: Tue, 07 Apr 2009 10:28:27 -0700
7, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
7, 39 -- Reply-to: larry.ackerman-at-ucsf.edu
7, 39 -- Organization: UCSF, NeuroAnatomy
7, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
7, 39 -- MIME-Version: 1.0
7, 39 -- To: Microscopy-at-microscopy.com
7, 39 -- Subject: Re: [Microscopy] storing slides - section info
7, 39 -- References: {200904071609.n37G9LNd032336-at-ns.microscopy.com}
7, 39 -- In-Reply-To: {200904071609.n37G9LNd032336-at-ns.microscopy.com}
7, 39 -- X-OriginalArrivalTime: 07 Apr 2009 17:28:33.0997 (UTC)
7, 39 -- FILETIME=[46F957D0:01C9B7A6]
7, 39 -- X-Brightmail-Tracker: AAAAAQ4wtX4=
7, 39 -- X-WSS-ID: 65C552C81OG68421-01-01
7, 39 -- Content-Type: text/plain;
7, 39 -- charset=iso-8859-1;
7, 39 -- format=flowed
7, 39 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 7 Apr 2009 13:11:41 -0500
Subject: [Microscopy] Re: SEM: Locating bacteria in fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 7, 2009, at 9:09 AM, TindallR-at-missouri.edu wrote:

} This comes under "Looking for small things in large places".
}
} One of our clients has determined the presence, but not the
} location, of
} bacteria in mushroom fruiting bodies. The mushrooms are about the
} size
} of the common white mushrooms you buy at the grocery store. He
} wants to
} know where the bugs be.
}
} We have tried breaking pieces off the mushroom and viewing them in an
} environmental SEM and, while we found all sorts of neat reproductive
} stuff, no bacteria were found. To search systematically through an
} entire fruiting body with no clue as to where the wee beasties might
} be
} can be done, but it's going to be expensive and time-consuming,
} especially since we don't know their physical appearance.
}
} TEM seems completely impractical for this for obvious reasons (if not,
} please enlighten me).
}
} So, if anyone has ideas on looking for needles in a fungal haystack,
} I'm
} all ears. Real ears, not wood ears.


Dear Randy,
I would try fluorescence light microscopy to scan large volumes of
the shrooms, then look at the areas of interest by SEM if necessary.
Of course, this supposes that your client can label the bacteria
specifically with a fluorescent probe. Since the presence of the
bacteria was determined, perhaps the process that showed the presence
of bacteria could be used to provide a suitable label--it all depends
on how the bacteria were identified.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Tue Apr 7 13:11:41 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37IBerV017448
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 13:11:41 -0500
6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id B0DC166E188D
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:11:39 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195])
6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id BA10666E4191
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:11:38 -0700 (PDT)
6, 22 -- Message-Id: {315BC34D-3B3A-4127-BD09-4741B868798F-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200904071609.n37G9kKi000827-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] SEM: Locating bacteria in fungi
6, 22 -- Date: Tue, 7 Apr 2009 11:11:38 -0700
6, 22 -- References: {200904071609.n37G9kKi000827-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 7 Apr 2009 13:19:51 -0500
Subject: [Microscopy] Bacteria and fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

Thanks for all the suggestions on locating little bugs in big mushrooms.
The consensus seems to be using paraffin sections with appropriate
staining and/or fluorescence to located the general location, then
EM'ing in on the critters.

That's what happens when you get in an electron rut, I guess. I forgot
to think outside of it. Thanks to everyone for the ideas!

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
8, 27 -- From TindallR-at-missouri.edu Tue Apr 7 13:19:51 2009
8, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37IJnFe025199
8, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 13:19:50 -0500
8, 27 -- X-IronPort-Anti-Spam-Filtered: true
8, 27 -- X-IronPort-Anti-Spam-Result: ApoEAGw220nRauUp/2dsb2JhbAC/ZQEJhxmITYJIgTMG
8, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
8, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 13:19:49 -0500
8, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 27 -- Tue, 7 Apr 2009 13:19:49 -0500
8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 27 -- Content-class: urn:content-classes:message
8, 27 -- MIME-Version: 1.0
8, 27 -- Content-Type: text/plain;
8, 27 -- charset="us-ascii"
8, 27 -- Subject: Bacteria and fungi
8, 27 -- Date: Tue, 7 Apr 2009 13:19:48 -0500
8, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FD7-at-UM-XMAIL08.um.umsystem.edu}
8, 27 -- X-MS-Has-Attach:
8, 27 -- X-MS-TNEF-Correlator:
8, 27 -- Thread-Topic: Bacteria and fungi
8, 27 -- Thread-Index: Acm3rW+NpIvKBwX2QueRdSP2b71AqQ==
8, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
8, 27 -- To: {microscopy-at-microscopy.com}
8, 27 -- X-OriginalArrivalTime: 07 Apr 2009 18:19:49.0203 (UTC) FILETIME=[6FF08A30:01C9B7AD]
8, 27 -- Content-Transfer-Encoding: 8bit
8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37IJnFe025199
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Tue, 7 Apr 2009 14:40:02 -0500
Subject: [Microscopy] Manuals for S-570 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers
Thank you for your overwhelming support - the manuals have been spoken for

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935


-----Original Message-----
X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
Sent: Tuesday, April 07, 2009 11:56 AM
To: rjharris-at-uwo.ca

Dear Listers
I have a complete set of manuals (installation, service and user) for a
Hitachi S-570 SEM available free - if this is of interest to you please
contact me off list

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935





==============================Original Headers==============================
7, 26 -- From rjharris-at-uwo.ca Tue Apr 7 10:48:28 2009
7, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n37FmRld007967
7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:48:28
-0500
7, 26 -- MIME-version: 1.0
7, 26 -- Content-type: text/plain; charset=iso-8859-1
7, 26 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri
[172.29.32.41])
7, 26 -- by zeppo.mail.uwo.pri
7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008;
32bit))
7, 26 -- with ESMTP id {0KHQ0085WMKPTYI0-at-zeppo.mail.uwo.pri} for
7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT)
7, 26 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
7, 26 -- by zeppo.mail.uwo.pri
7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008;
32bit))
7, 26 -- with ESMTPSA id {0KHQ00MXHMKPR650-at-zeppo.mail.uwo.pri} for
7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT)
7, 26 -- From: Richard Harris {rjharris-at-uwo.ca}
7, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com}
7, 26 -- Subject: Manuals for S-570 SEM
7, 26 -- Date: Tue, 07 Apr 2009 11:48:26 -0400
7, 26 -- Message-id: {002601c9b798$4a45f320$ded1d960$-at-ca}
7, 26 -- X-Mailer: Microsoft Office Outlook 12.0
7, 26 -- Content-language: en-us
7, 26 -- Thread-index: Acm3mEoiKth8qgRsTZSHCx9a+yeIWw==
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n37FmRld007967
==============================End of - Headers==============================



==============================Original Headers==============================
18, 29 -- From rjharris-at-uwo.ca Tue Apr 7 14:40:01 2009
18, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37Je1rH016051
18, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 14:40:01 -0500
18, 29 -- MIME-version: 1.0
18, 29 -- Content-type: text/plain; charset=iso-8859-1
18, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39])
18, 29 -- by harpo.mail.uwo.pri
18, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
18, 29 -- with ESMTP id {0KHQ00K0MXAN18I0-at-harpo.mail.uwo.pri} for
18, 29 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 15:39:59 -0400 (EDT)
18, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
18, 29 -- by harpo.mail.uwo.pri
18, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
18, 29 -- with ESMTPSA id {0KHQ00LEFXANWL00-at-harpo.mail.uwo.pri} for
18, 29 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 15:39:59 -0400 (EDT)
18, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
18, 29 -- To: rjharris-at-uwo.ca
18, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
18, 29 -- References: {200904071556.n37Fu0dD020190-at-ns.microscopy.com}
18, 29 -- In-reply-to: {200904071556.n37Fu0dD020190-at-ns.microscopy.com}
18, 29 -- Subject: RE: [Microscopy] Manuals for S-570 SEM
18, 29 -- Date: Tue, 07 Apr 2009 15:39:59 -0400
18, 29 -- Message-id: {007301c9b7b8$a31be6b0$e953b410$-at-ca}
18, 29 -- X-Mailer: Microsoft Office Outlook 12.0
18, 29 -- Content-language: en-us
18, 29 -- Thread-index: Acm3mV3lQBKfOarpQKKSULiW42GaLgAHyDOQ
18, 29 -- Content-Transfer-Encoding: 8bit
18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37Je1rH016051
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Wed, 8 Apr 2009 04:44:42 -0500
Subject: [Microscopy] SEM: Locating bacteria in fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Using ESEM to find bacteria could be difficult if they are embedded in
XPS/slime ie you may not recognise them. It might be worth fixing and
dehydrating in solvent to remove slime leaving naked bacteria.

Dave

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: 07 April 2009 17:14
To: David Patton

This comes under "Looking for small things in large places".

One of our clients has determined the presence, but not the location, of
bacteria in mushroom fruiting bodies. The mushrooms are about the size
of the common white mushrooms you buy at the grocery store. He wants to
know where the bugs be.

We have tried breaking pieces off the mushroom and viewing them in an
environmental SEM and, while we found all sorts of neat reproductive
stuff, no bacteria were found. To search systematically through an
entire fruiting body with no clue as to where the wee beasties might be
can be done, but it's going to be expensive and time-consuming,
especially since we don't know their physical appearance.

TEM seems completely impractical for this for obvious reasons (if not,
please enlighten me).

So, if anyone has ideas on looking for needles in a fungal haystack, I'm
all ears. Real ears, not wood ears.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original
Headers==============================
9, 27 -- From TindallR-at-missouri.edu Tue Apr 7 11:09:39 2009
9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu
(mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n37G9cl7000497
9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009
11:09:39 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result:
ApoEALkX20nRauUo/2dsb2JhbAC/VAEJhwGITYJKgTMG
9, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
([209.106.229.40])
9, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009
11:09:36 -0500
9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by
um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 27 -- Tue, 7 Apr 2009 11:09:35 -0500
9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 27 -- Content-class: urn:content-classes:message
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Type: text/plain;
9, 27 -- charset="us-ascii"
9, 27 -- Subject: SEM: Locating bacteria in fungi
9, 27 -- Date: Tue, 7 Apr 2009 11:09:35 -0500
9, 27 -- Message-ID:
{91108EF9255B394CBF8B7E3789814A4103CD7FC9-at-UM-XMAIL08.um.umsystem.edu}
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- Thread-Topic: SEM: Locating bacteria in fungi
9, 27 -- Thread-Index: Acm3mz5qeov9y91NQHG6WZ82emeIxA==
9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 27 -- To: {microscopy-at-microscopy.com}
9, 27 -- X-OriginalArrivalTime: 07 Apr 2009 16:09:35.0394 (UTC)
FILETIME=[3E8BE820:01C9B79B]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n37G9cl7000497
==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
21, 34 -- From David.Patton-at-uwe.ac.uk Wed Apr 8 04:44:42 2009
21, 34 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
21, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n389if8n025417
21, 34 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 04:44:41 -0500
21, 34 -- Received: from (unknown [164.11.132.62]) by mailapp04.uwe.ac.uk with smtp
21, 34 -- id 5b24_e132d1ae_2421_11de_8a6c_00142223915c;
21, 34 -- Wed, 08 Apr 2009 10:44:40 +0100
21, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
21, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
21, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
21, 34 -- 2005)) with SMTP id {0KHS00C4Y0EFVF-at-mta02.uwe.ac.uk} for
21, 34 -- microscopy-at-microscopy.com; Wed, 08 Apr 2009 10:44:39 +0100 (BST)
21, 34 -- Date: Wed, 08 Apr 2009 10:42:03 +0100
21, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk}
21, 34 -- Subject: RE: [Microscopy] SEM: Locating bacteria in fungi
21, 34 -- In-reply-to: {200904071613.n37GDifN009562-at-ns.microscopy.com}
21, 34 -- To: TindallR-at-missouri.edu
21, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB048C5850-at-egen-uwe01}
21, 34 -- MIME-version: 1.0
21, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
21, 34 -- Content-type: text/plain; charset=us-ascii
21, 34 -- Content-class: urn:content-classes:message
21, 34 -- Thread-topic: [Microscopy] SEM: Locating bacteria in fungi
21, 34 -- Thread-index: Acm3m9+PGFaFp8iwTRGgG8HlVPUakQAkg4Ug
21, 34 -- X-MS-Has-Attach:
21, 34 -- X-MS-TNEF-Correlator:
21, 34 -- References: {200904071613.n37GDifN009562-at-ns.microscopy.com}
21, 34 -- X-NAIMIME-Disclaimer: 1
21, 34 -- X-NAIMIME-Modified: 1
21, 34 -- X-NAI-Spam-Score: 0
21, 34 -- X-NAI-Spam-Rules: 1 Rules triggered
21, 34 -- RV3248=0
21, 34 -- Content-Transfer-Encoding: 8bit
21, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n389if8n025417
==============================End of - Headers==============================




From: dingbiocmu-at-yahoo.com
Date: Wed, 8 Apr 2009 06:37:19 -0500
Subject: [Microscopy] viaWWW: protocol for chromosomes aberrations test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dingbiocmu-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dingbiocmu-at-yahoo.com
Name: heidi

Organization: Central Mindanao University

Title-Subject: [Filtered] request for protocol for chromosomes
aberrations test Using rat bone marrow and rats spermatocytes.

Question:
dear listers,

will anyone be kind enough to email me a complete protocols for
chromosomes aberrations test using rat bone marrow and rats
spermatocytes. Thanks a lot.

ding


Login Host: 203.111.229.158
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Apr 8 06:37:19 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n38BbIRn010751
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 06:37:18 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c6023cd9673d-at-[206.69.208.22]}
9, 11 -- Date: Wed, 8 Apr 2009 06:37:17 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: dingbiocmu-at-yahoo.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: protocol for chromosomes aberrations test
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Wed, 8 Apr 2009 10:06:39 -0500
Subject: [Microscopy] Re: storing slides - section info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I was performing EM immunostaining in industry, we always assumed that tissue blocks in resin were preserved, but as soon as you section the block, the immediate block face and the sections will undergo changes as a result of being exposed to air and light (where applicable).

In histology, some people will immunostain paraffin embedded slides that are many months old, but our careful time-course comparisons revealed that although immunostaining older slides produced a staining product, the sensitivity was greatly reduced after the first 24-36 hours, and further degradation occurred some 1-2 weeks later. Many antibodies worked fine after a month or two.

I believe that similar results were observed with resin embedding, but I can't remember if I actually read a paper on it, or if we extrapolated the conclusion from histology IHC results.

Someone must have published results on this, don't you think?

Regards,
Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu]
Sent: Tuesday, April 07, 2009 1:42 PM
To: Sobocinski, Gregg

Tissue should be relatively stable in epoxy even at room temperature.
Consider Kristina Micheva's (Stephen Smith Lab) Array Tomography
technique where LR White sections are repeatedly immunolabeled and each
label is eluted with .2M NaOH and .1% SDS before the next label is
applied. Of course, there is always an antigen that is the exception.
Larry

beth-at-plantbio.uga.edu wrote:
}
Hi all,
How long can slides with sections be stored before they are used for
immunolabeling? 6 months in the refrigerator? or longer? or is it a
bad idea to wait?
I usually cut sections then label the next day but someone here would
like to store the slides for awhile if that is an okay practice.

Any advice would be greatly appreciated.
thanks,
Beth
}
} PS - I forgot to say that the tissue is embedded in LR White.
}
} **********************************************************************
} Beth Richardson
} Electron Microscopy Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} http://www.plantbio.uga.edu/emlab/
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} *******************************************************************
}
} "And it's only the giving that makes you what you are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
} ***************************************************************************
} The Friends of the Marine Institute - Join Today!
} www.friendsofugami.com
}
}
}
}
}
} ==============================Original Headers==============================
} 11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009
} 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
} 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252
} 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500
} 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
} 11, 19 -- (authenticated user beth-at-plantbio.uga.edu)
} 11, 19 -- by dogwood.plantbio.uga.edu
} 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
} 11, 19 -- for microscopy-at-microscopy.com;
} 11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400
} 11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu}
} 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
} 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
} 11, 19 -- Content-Transfer-Encoding: 7bit
} 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 11, 19 -- Subject: storing slides - section info
} 11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400
} 11, 19 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400


==============================Original Headers==============================
7, 39 -- From Larry.Ackerman-at-ucsf.edu Tue Apr 7 12:29:39 2009
7, 39 -- Received: from emfmcb01.ucsfmedicalcenter.org (EMFMCB01.ucsfmedicalcenter.org [64.54.46.97])
7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37HTajp002078
7, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 12:29:37 -0500
7, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with
7, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2));
7, 39 -- Tue, 07 Apr 2009 10:28:34 -0700
7, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B
7, 39 -- X-AuditID: 403623d2-a7709bb0000017ee-a5-49db8d42a758
7, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org (
7, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by
7, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id
7, 39 -- 63FC115BD for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:28:34
7, 39 -- -0700 (PDT)
7, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by
7, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.3959);
7, 39 -- Tue, 7 Apr 2009 10:28:34 -0700
7, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
7, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Tue, 7 Apr
7, 39 -- 2009 10:28:33 -0700
7, 39 -- Message-ID: {49DB8D3B.60402-at-ucsf.edu}
7, 39 -- Date: Tue, 07 Apr 2009 10:28:27 -0700
7, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu}
7, 39 -- Reply-to: larry.ackerman-at-ucsf.edu
7, 39 -- Organization: UCSF, NeuroAnatomy
7, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
7, 39 -- MIME-Version: 1.0
7, 39 -- To: Microscopy-at-microscopy.com
7, 39 -- Subject: Re: [Microscopy] storing slides - section info
7, 39 -- References: {200904071609.n37G9LNd032336-at-ns.microscopy.com}
7, 39 -- In-Reply-To: {200904071609.n37G9LNd032336-at-ns.microscopy.com}
7, 39 -- X-OriginalArrivalTime: 07 Apr 2009 17:28:33.0997 (UTC)
7, 39 -- FILETIME=[46F957D0:01C9B7A6]
7, 39 -- X-Brightmail-Tracker: AAAAAQ4wtX4=
7, 39 -- X-WSS-ID: 65C552C81OG68421-01-01
7, 39 -- Content-Type: text/plain;
7, 39 -- charset=iso-8859-1;
7, 39 -- format=flowed
7, 39 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================


==============================Original Headers==============================
19, 25 -- From greggps-at-umich.edu Wed Apr 8 10:06:38 2009
19, 25 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n38F6aNP000399
19, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 10:06:37 -0500
19, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by
19, 25 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Wed, 8 Apr
19, 25 -- 2009 11:06:34 -0400
19, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
19, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
19, 25 -- Date: Wed, 8 Apr 2009 11:06:33 -0400
19, 25 -- Subject: RE: [Microscopy] storing slides - section info
19, 25 -- Thread-Topic: [Microscopy] storing slides - section info
19, 25 -- Thread-Index: Acm3qCK6Xcl2grbIT3mlJvMOaGQaaAAqQbJw
19, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA393401816E2F36-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
19, 25 -- References: {200904071741.n37Hfjgo014386-at-ns.microscopy.com}
19, 25 -- In-Reply-To: {200904071741.n37Hfjgo014386-at-ns.microscopy.com}
19, 25 -- Accept-Language: en-US
19, 25 -- Content-Language: en-US
19, 25 -- X-MS-Has-Attach:
19, 25 -- X-MS-TNEF-Correlator:
19, 25 -- acceptlanguage: en-US
19, 25 -- Content-Type: text/plain; charset="us-ascii"
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n38F6aNP000399
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 8 Apr 2009 14:54:08 -0500
Subject: [Microscopy] ISA legacy board support

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gregg

I had always assumed that, as you say, the best immuno results were
from fresh sections. I further understood that resin embedded sections
were fairly impervious to at least some immuno stains (especially of
course immuno-gold) and so only the exposed cut surface of the section
would present antigens to the label.

If the fewer available antigens in a stored resin section are greatly
reduced then I would assume that staining might be more affected than
a de-waxed section (or possibly a partially preserved cryo-section).

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
X-from: greggps-at-umich.edu

Here is a link to a company that makes motherboards
with CPU, PCI and ISA slot(s):

http://www.cyberresearch.com/store/industrial-computers-rugged-pcs/motherboards-mobo/?Sa136=4564&S725f=&S512e=1354&S8764=4&S1466=1&S558c=&S8d1d=3055&Sfc04=&S141b=&S576c=&S3476=&lod=1&categoryid=71&df=

A bit pricey but perhaps more cost effective than replacing
an entire system because of no ISA slot.

gary g.


==============================Original Headers==============================
5, 17 -- From gary-at-gaugler.com Wed Apr 8 14:54:07 2009
5, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n38Js7Mw007771
5, 17 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 14:54:07 -0500
5, 17 -- Message-Id: {200904081954.n38Js7Mw007771-at-ns.microscopy.com}
5, 17 -- Received: (qmail 2015 invoked from network); 8 Apr 2009 12:50:49 -0700
5, 17 -- Received: by simscan 1.1.0 ppid: 2012, pid: 2013, t: 0.1333s
5, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
5, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
5, 17 -- by smtp2 with SMTP; 8 Apr 2009 12:50:49 -0700
5, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
5, 17 -- Date: Wed, 08 Apr 2009 12:53:54 -0700
5, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
5, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
5, 17 -- Subject: ISA legacy board support
5, 17 -- Mime-Version: 1.0
5, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jinsong-wu-at-northwestern.edu
Date: Wed, 8 Apr 2009 17:44:07 -0500
Subject: [Microscopy] viaWWW: Hitachi HF-2000 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jinsong-wu-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jinsong-wu-at-northwestern.edu
Name: jinsong wu

Organization: northwestern university

Title-Subject: [Filtered] Hitachi HF-2000 available

Question: Hi All,

Due to the space limitation, the Hitachi HF-2000 transmission
electron microscope installed at Northwestern University (currently
in very good working condition) is now available
at good price.
Please write back you have interests and need more information. It
has cold field emission gun (high brightness
and energy resolution) and super HRTEM and EDS capability.
Many thanks.

with best regards,

jinsong




Login Host: 129.105.37.144
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Wed Apr 8 17:44:07 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n38Mi59F001298
12, 11 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 17:44:07 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240801c602d9170675-at-[206.69.208.22]}
12, 11 -- Date: Wed, 8 Apr 2009 17:44:04 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: jinsong-wu-at-northwestern.edu (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Hitachi HF-2000 available
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: allan.mitchell-at-stonebow.otago.ac.nz
Date: Wed, 8 Apr 2009 17:44:39 -0500
Subject: [Microscopy] viaWWW: FCS pump speed problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both allan.mitchell-at-stonebow.otago.ac.nz as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: allan.mitchell-at-stonebow.otago.ac.nz
Name: Allan Mitchell

Organization: Otago University

Title-Subject: [Filtered] FCS pump speed problem

Question: Hi All

We are having a problem with the liquid nitrogen pump speed on our
Leica FCS cryosectioning system (1993 vintage). Does anyone have a
copy of the schematics for the FC S controller boards and
interconnecting wiring diagram and also the pump control board that
they can send as a PDF file, or fax to us.

That would help us a lot to get to the bottom of our problem.

Regards

Allan

Allan Mitchell
Microscopy Otago - Electron Microscopy
c/- Department of Anatomy and Structural Biology
Otago School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone 00 64(03) 479 5642 or 479 7301
Fax 0064 (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
NZ Microscopy Society: http://microscopynz.co.nz/


Login Host: 139.80.34.166
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Wed Apr 8 17:44:38 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n38Mia0Z001991
14, 11 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 17:44:38 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240802c602d93e0fb6-at-[206.69.208.22]}
14, 11 -- Date: Wed, 8 Apr 2009 17:44:35 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: allan.mitchell-at-stonebow.otago.ac.nz (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: FCS pump speed problem
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 9 Apr 2009 12:42:34 -0500
Subject: [Microscopy] motorized stage replacement needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are currently using a automated stage (x, y and z axis) on our Zeiss
Axioplan reflected light scope. Unfortunately the stage is, judging by its
groaning, reaching the end of it's product life.

Does anyone know of a manufacturer or company what makes or refurbishes
motor driven stages? The plot complication is the stage must be comparable
with Clemex drivers and software.

Vendors are welcome and encouraged to respond. I can be reached at
frank_karl-at-lincolnelectric.com

Thanks in advance..............
Frank Karl

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
7, 21 -- From frank_karl-at-lincolnelectric.com Thu Apr 9 12:42:34 2009
7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39HgXTa019061
7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 12:42:33 -0500
7, 21 -- Subject: motorized stage replacement needed
7, 21 -- To: Microscopy-at-microscopy.com
7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 21 -- Message-ID: {OFF749E3EF.6277FB97-ON85257593.00605D89-85257593.0061435E-at-lincolnelectric.com}
7, 21 -- Date: Thu, 9 Apr 2009 13:42:19 -0400
7, 21 -- From: Frank_Karl-at-lincolnelectric.com
7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM,
7, 21 -- CD-MIME complete at 04/09/2009 01:42:17 PM,
7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM,
7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM,
7, 21 -- Serialize complete at 04/09/2009 01:42:17 PM
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: linda_wang777-at-yahoo.com
Date: Thu, 9 Apr 2009 13:39:24 -0500
Subject: [Microscopy] Denton Vacuum Evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All Members,

I am looking for an used Denton DV-502 or DV-502A Vacuum Evaporator at low price. If you have it, please email me at linda_wang777-at-yahoo.com.

Thank you.


Linda




==============================Original Headers==============================
8, 20 -- From linda_wang777-at-yahoo.com Thu Apr 9 13:39:24 2009
8, 20 -- Received: from web44708.mail.sp1.yahoo.com (web44708.mail.sp1.yahoo.com [69.147.94.104])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n39IdOg8002259
8, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 9 Apr 2009 13:39:24 -0500
8, 20 -- Received: (qmail 78202 invoked by uid 60001); 9 Apr 2009 18:39:23 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1239302363; bh=mcZZydBGKWLXe0A8pClvAsDhlufVswcHjU1mHNPdvhM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=xajj3uTHRXxr63vEZF+SwqzdgXlqpAGhWhKUk9CaDdlhOLyJryVDhWihQOBIppHTNcmR/kEmptm1vNH2cufic3G+4waVDALhB+rWA4KAIX0vc1BUCkB2vm0tuEh36ivq8cnzN/D5BuRVvaPXL/zMEGcZApGNAh6HDZqItnZ7cVE=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=WbhPhYXb7HxdZfd2ZrvshOA7NFvs5cr9GBfXwzCwp+tz1RByAq6E0kqBINyQHioi1rsCZCVdUoHU88QNmvyxXjJt02v9ALNwCtAPqccX3xxJisfabIURFJfjZy1LXkR/iyEvZGhPKTRyhogxx+4zHeqAYpd/+NtUkzKkTyeXY74=;
8, 20 -- Message-ID: {934557.76899.qm-at-web44708.mail.sp1.yahoo.com}
8, 20 -- X-YMail-OSG: 3VRkDZ8VM1nN4UYQwy4ecfYrUnpUUjt2DhHMJQ9qNLYNIyI6AT4TqHnfxwK.WdAOtp9Y.dsln1HxKhWN2lshbpLli3OZxlffGUseuMSJaT9UhcBuxh.w8fHMi8BS065SE0VZdV7cllCgI3.XgnNe7ZcfPUppR7x.pOtKM82D4ZJseYXUMsgCwrBUdgIG34dWPUhqqU4BMzGx.Ffe5wBHwLLioBpRtVsbJAWb4XtP4tdnvj5HWwq_bVqdQPq62vsJeHMVXhG7z2evVNCinCTeuNWERXJBpzcf9cQDoKrxuBUu2n_1Beun
8, 20 -- Received: from [75.140.108.220] by web44708.mail.sp1.yahoo.com via HTTP; Thu, 09 Apr 2009 11:39:23 PDT
8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
8, 20 -- Date: Thu, 9 Apr 2009 11:39:23 -0700 (PDT)
8, 20 -- From: Linda Wang {linda_wang777-at-yahoo.com}
8, 20 -- Subject: Denton Vacuum Evaporator
8, 20 -- To: Microscopy-at-Microscopy.Com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: dlowry-at-asu.edu
Date: Thu, 9 Apr 2009 18:47:12 -0500
Subject: [Microscopy] viaWWW: sample preparation , cross-sectional analysis of fish fins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] sample preparation question

Question: I was contacted by a researcher who wants to do
cross-sectional analysis of fish fin rays, which are somewhat brittle
structures. He wants to be able to use light microscopy to image the
rings that are present within the fin rays. I assume the rays would
have to be embedded, then cut and perhaps polished.

I would like to ask any list members with related experience in this
area for advice--how to fix, process, what type of resin, etc. Thank
you,

Login Host: 149.169.100.108
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Apr 9 18:47:12 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39NlC5w029139
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 18:47:12 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240803c6043953963f-at-[206.69.208.22]}
7, 11 -- Date: Thu, 9 Apr 2009 18:47:11 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: dlowry-at-asu.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: sample preparation , cross-sectional analysis of fish fins
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Thu, 9 Apr 2009 18:47:57 -0500
Subject: [Microscopy] viaWWW: Correction Senior Scientist Position SAIC - Frederick

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David Parmiter

Organization: SAIC - Frederick

Title-Subject: [Filtered] Correction

Question: Hello all -

Sorry about this, but there is a small correction to my previous post:

The correct posting number is 138805.

Thanks!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Apr 9 18:47:57 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39NluGk030127
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 18:47:56 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240804c6043990a47d-at-[206.69.208.22]}
9, 11 -- Date: Thu, 9 Apr 2009 18:47:55 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Correction Senior Scientist Position SAIC - Frederick
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Thu, 9 Apr 2009 18:48:02 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David Parmiter

Organization: SAIC - Frederick

Title-Subject: [Filtered] Correction

Question: Hello all -

Sorry about this, but there is a small correction to my previous post:

The correct posting number is 138805.

Thanks!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Apr 9 18:48:02 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39NlxOS030175
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 18:48:01 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c604389268d7-at-[206.69.208.22]}
9, 11 -- Date: Thu, 9 Apr 2009 18:47:58 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: parmiterd-at-mail.nih.gov (by way of Nestor J. Zaluzec)
9, 11 -- Subject: [Filtered] MicroscopyListserverviaWWW:
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Thu, 9 Apr 2009 18:49:09 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David Parmiter

Organization: SAIC - Frederick

Title-Subject: [Filtered] Correction

Question: Hello all -

Sorry about this, but there is a small correction to my previous post:

The correct posting number is 138805.

Thanks!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Apr 9 18:49:09 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39Nn8mX001827
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 18:49:09 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c604389268d7-at-[206.69.208.22]}
9, 11 -- Date: Thu, 9 Apr 2009 18:47:58 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: parmiterd-at-mail.nih.gov (by way of Nestor J. Zaluzec)
9, 11 -- Subject: [Filtered] MicroscopyListserverviaWWW:
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 9 Apr 2009 23:04:24 -0500
Subject: [Microscopy] Rhetorical question about a SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Group:

Looking back to LaB6 SEMs, what would you
suggest as a viable, reliable and supportable
tool? This should include computer control,
5-axis motorized ability, digital capture,
turbo vacuum, load lock as an asset (not for
wafers and not necessary), 3.1mm pin stub accommodation, easy
maintenance, mag to 500KX. Zero power when
off. Pin stubs at 12mm diameter up to 25mm diameter.
Think of Hitachi Type II stage. There is no
need for huge stage size.

Maker or brand is of no significance. Computer
control and digital capture are essential. What I
do not know is the time frame for transition from
Polaroids to digital. Resolution of about 2nm at
20KV is good..lesser is considered. But this
is not a hard and fast figure.

If one looks at the old models of the major makers,
what are the ones that come to the top? I'd say that
ten to twelve year of age are old but could be viable.

And, what do you think these tools would be worth or
priced at?

Any thoughts?

Off-line is probably good.

gary g.


==============================Original Headers==============================
9, 17 -- From gary-at-gaugler.com Thu Apr 9 23:04:24 2009
9, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3A44O3S028055
9, 17 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 23:04:24 -0500
9, 17 -- Message-Id: {200904100404.n3A44O3S028055-at-ns.microscopy.com}
9, 17 -- Received: (qmail 6860 invoked from network); 9 Apr 2009 21:01:00 -0700
9, 17 -- Received: by simscan 1.1.0 ppid: 6853, pid: 6856, t: 0.1434s
9, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 17 -- by smtp2 with SMTP; 9 Apr 2009 21:01:00 -0700
9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 17 -- Date: Thu, 09 Apr 2009 21:04:10 -0700
9, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
9, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 17 -- Subject: Rhetorical question about a SEM
9, 17 -- Mime-Version: 1.0
9, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: fahayes-at-ucdavis.edu
Date: Fri, 10 Apr 2009 13:42:09 -0500
Subject: [Microscopy] suggestions for an instrument electronic signup calender

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are a multi user facility using a web event calender for instrument
reservations. We are changing this calender system and would like to get
suggestions from those of you using a calender system designed for
instruments. Any suggestions

Thank you

--
Fred A. Hayes
Manager, Central Facilities
Department of Chemical Engineering and Material Sciences
3118 Bainer Hall
Bainer Hall Drive
UC Davis
Davis, CA 95616
530-752-0284 office
530-754-6350 fax
707-761-9045 cell
fahayes-at-ucdavis.edu
http://www.matscicf.ucdavis.edu/









==============================Original Headers==============================
11, 19 -- From fahayes-at-ucdavis.edu Fri Apr 10 13:42:08 2009
11, 19 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3AIg80N013162
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 13:42:08 -0500
11, 19 -- Received: from [169.237.230.148] ([169.237.230.148])
11, 19 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n3AIg7x3012353
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 11:42:07 -0700 (PDT)
11, 19 -- Message-ID: {49DF92FF.6050607-at-ucdavis.edu}
11, 19 -- Date: Fri, 10 Apr 2009 11:42:07 -0700
11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu}
11, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
11, 19 -- MIME-Version: 1.0
11, 19 -- To: Microscopy-at-Microscopy.Com
11, 19 -- Subject: suggestions for an instrument electronic signup calender
11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av8
11, 19 -- X-Virus-Status: Clean
11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 10 Apr 2009 14:16:41 -0500
Subject: [Microscopy] Re: suggestions for an instrument electronic signup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fred,

You didn't say what you are using nor why you are changing, but have a
look at our calendar:
https://bcrc.bio.umass.edu/cmreserve/day.php?day
I'm fairly sure outsiders can view it.

It goes by "MRBS" (MeetingRoomBookingSystem) -
http://mrbs.sourceforge.net/

We like it. I don't manage it; the guru (Steve Brewer) at our BCRC set
it up. http://www.bio.umass.edu/biology/bcrc/

We used a system based on a wiki before (again, Steve set it up). That
was nice in a different way - very flexible and you could leave messages
and post notices. It also left an edit record of old versions which was
sometimes handy to mediate disputes....

Dale

fahayes-at-ucdavis.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} We are a multi user facility using a web event calender for instrument
} reservations. We are changing this calender system and would like to get
} suggestions from those of you using a calender system designed for
} instruments. Any suggestions
}
} Thank you
}

==============================Original Headers==============================
7, 23 -- From dac-at-research.umass.edu Fri Apr 10 14:16:40 2009
7, 23 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3AJGeqV028687
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Apr 2009 14:16:40 -0500
7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
7, 23 -- (authenticated bits=0)
7, 23 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n3AJGdkJ024523
7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Apr 2009 15:16:40 -0400
7, 23 -- Message-ID: {49DF9B49.5050602-at-research.umass.edu}
7, 23 -- Date: Fri, 10 Apr 2009 15:17:29 -0400
7, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
7, 23 -- Reply-To: dac-at-research.umass.edu
7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.21) Gecko/20090303 SeaMonkey/1.1.15
7, 23 -- MIME-Version: 1.0
7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 23 -- Subject: Re: [Microscopy] suggestions for an instrument electronic signup
7, 23 -- calender
7, 23 -- References: {200904101848.n3AImqQR021412-at-ns.microscopy.com}
7, 23 -- In-Reply-To: {200904101848.n3AImqQR021412-at-ns.microscopy.com}
7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 23 -- Content-Transfer-Encoding: 7bit
7, 23 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: fwang-at-phys.ualberta.ca
Date: Sun, 12 Apr 2009 08:52:57 -0500
Subject: [Microscopy] viaWWW: TEM sample preparation by Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Fred,

You may have interest in trying our Facility Online Manager (FOM) software
for your facility management. FOM has the following features you may
appreciate:

. Written in Java and use Microsoft SQL engine. FOM is modularized and
easy to expand or customize to meet your facility needs.
. Flexible scheduling and fee structure. For each instrument, manager
may set different schedule increment, daytime start/end, schedule
limitations, and billing policy. Instruments may be charged per use, per
actual used time, or combination of reserved and actual used time. Charge
calculation is rounded to one minute.
. Reservations, cancellations, modifications, early logons, late-comers,
extended sessions, conflicting reservations, no-shows, forgot logoffs,
auto-logoffs, reserve for users, training sessions, service sessions, repair
sessions... All scenarios are covered.
. Unlimited discount structures for special user groups.
. Consumable sales and inventory. For each instrument, manager may set
unlimited consumables associated and charge to user separately. Separate
consumable sales also available. Managers receive warning messages when
consumable is running out.
. Operation manuals online. For each instrument, manager may set
unlimited documents for users to download.
. Solid access control. FOM uses (optional) hardware relay switches to
control access to the instruments. This ensures the user logon FOM when
every time the instrument is used.
. FOM has been customized for Northwestern University to use of NetID
login. It is possible to integrate FOM with any existing online passport
system (need inter-server communication).
. Optional magnetic card login.
. FOM has been customized for Northwestern University to be fully
compatible with NUFS financial system. It is possible to customize FOM to
generate financial reports following any established accounting system.
. FOM provides fairly complicated and flexible rules to validate user
financial accounts. For Northwestern University, FOM dynamically validates
user NUFS strings against NU financial system. Automatic email warnings will
be sent when user account expires
. Numerous reports. Customized reports are available upon requests.
. Clear administration structure. System admin, facility admin,
instrument manager, supervisor, and user.
. Easy user communications. Automatic notices, email lists.
. Easy to add new facilities. It takes one minute for System Admin to
add another entry in the database. Then Facility Admin will be able to login
and add new instruments. Existing users have access to the new facility
right away without separate registration of accounts.
. Easy to add new instruments. Just take a few minutes to configure
parameters for the new instruments.
. Complete control of usage records. Batch import usage records from
existing databases available.
. FOM has been licensed to more than ten laboratories at Northwestern
University and six other universities.
. Please visit http://www.FOMNetworks.com/ to see more details about
FOM.

If you want to try the software online, I can send you test login accounts.
Please feel free to contact me if you have questions regarding Facility
Online Manager software and related facility management issues.

Thank you,
Shuyou
_____________________________
Shuyou Li, Ph.D.
Electron Microscopist and IT Specialist
NUANCE Center
Northwestern University
2220 Campus Drive, Cook Hall RM#2036
Evanston, IL 60208-3108, USA
Ph: (847) 491-6723
Fax: (847) 467-6573
Email: syli-at-northwestern.edu
http://www.nuance.northwestern.edu/

-----Original Message-----
X-from: fahayes-at-ucdavis.edu [mailto:fahayes-at-ucdavis.edu]
Sent: Friday, April 10, 2009 1:47 PM
To: syli-at-northwestern.edu

We are a multi user facility using a web event calender for instrument
reservations. We are changing this calender system and would like to get
suggestions from those of you using a calender system designed for
instruments. Any suggestions

Thank you

--
Fred A. Hayes
Manager, Central Facilities
Department of Chemical Engineering and Material Sciences
3118 Bainer Hall
Bainer Hall Drive
UC Davis
Davis, CA 95616
530-752-0284 office
530-754-6350 fax
707-761-9045 cell
fahayes-at-ucdavis.edu
http://www.matscicf.ucdavis.edu/









==============================Original Headers==============================
11, 19 -- From fahayes-at-ucdavis.edu Fri Apr 10 13:42:08 2009
11, 19 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3AIg80N013162
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 13:42:08
-0500
11, 19 -- Received: from [169.237.230.148] ([169.237.230.148])
11, 19 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with
ESMTP id n3AIg7x3012353
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 11:42:07
-0700 (PDT)
11, 19 -- Message-ID: {49DF92FF.6050607-at-ucdavis.edu}
11, 19 -- Date: Fri, 10 Apr 2009 11:42:07 -0700
11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu}
11, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
11, 19 -- MIME-Version: 1.0
11, 19 -- To: Microscopy-at-Microscopy.Com
11, 19 -- Subject: suggestions for an instrument electronic signup calender
11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version
0.94.2 on av8
11, 19 -- X-Virus-Status: Clean
11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32
==============================End of - Headers==============================


==============================Original Headers==============================
22, 22 -- From syli-at-northwestern.edu Fri Apr 10 18:20:40 2009
22, 22 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ANKePH019160
22, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Apr 2009 18:20:40 -0500
22, 22 -- Received: from prep1 (interface.ms.northwestern.edu [129.105.37.115])
22, 22 -- by merle.it.northwestern.edu (Postfix) with ESMTP id AB8B9744F;
22, 22 -- Fri, 10 Apr 2009 18:20:40 -0500 (CDT)
22, 22 -- From: "Shuyou Li" {syli-at-northwestern.edu}
22, 22 -- To: {fahayes-at-ucdavis.edu}
22, 22 -- Cc: {Microscopy-at-microscopy.com}
22, 22 -- References: {200904101847.n3AIlHV1019240-at-ns.microscopy.com}
22, 22 -- In-Reply-To: {200904101847.n3AIlHV1019240-at-ns.microscopy.com}
22, 22 -- Subject: RE: [Microscopy] suggestions for an instrument electronic signup calender
22, 22 -- Date: Fri, 10 Apr 2009 18:20:38 -0500
22, 22 -- Message-ID: {0d0501c9ba32$f531e9f0$df95bdd0$-at-edu}
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain;
22, 22 -- charset="us-ascii"
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Mailer: Microsoft Office Outlook 12.0
22, 22 -- Thread-Index: Acm6DMXwNwUFvumiT2iswVzRa5bPxAAJaA0A
22, 22 -- Content-Language: en-us
==============================End of - Headers==============================

From gesiot-at-infinito.it Sun Apr 12 03:39:08 2009
Return-Path: {gesiot-at-infinito.it}
Received: from google.com ([95.58.145.119])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3C8d5XY017437
for {microscopylistserverarchive-at-microscopy.com} ; Sun, 12 Apr 2009 03:39:07 -0500
Received: from [123.140.39.80] (HELO google.com)
by sharplousywith.cn; Sun, 12 Apr 2009 14:39:00 +0700

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both fwang-at-phys.ualberta.ca as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: fwang-at-phys.ualberta.ca
Name: Feng

Organization: Brookhaven National Lab

Title-Subject: [Filtered] TEM sample preparation by Microtome

Question: Hi all,

We hope to prepare cross-section TEM samples from micron-size powder
(like transition metal oxides), and wonder if we can embed this kind
of powder into epoxy and cut it into thin slices (such as 20-40nm in
thickness) by some special mirotome.

Since I don't have any experience with microtome for TEM sample
preparation, any suggestions on possiblities, requirement, tricks,
would be appreciated.

Also we may think of buying the facilities if such kind of work can
be done with microtome, please let me know if you have any related
informaiton, thank you.

Feng



Login Host: 130.199.3.140
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Sun Apr 12 08:52:57 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3CDqt7s012053
12, 11 -- for {microscopy-at-microscopy.com} ; Sun, 12 Apr 2009 08:52:57 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c607a2a94a36-at-[206.69.208.22]}
12, 11 -- Date: Sun, 12 Apr 2009 08:52:55 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: fwang-at-phys.ualberta.ca (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: TEM sample preparation by Microtome
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Sun, 12 Apr 2009 18:19:46 -0500
Subject: [Microscopy] Miller indices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hoping to get input about a certain consensus on the issue of using
Miller indices for notation of crystallographic planes and directions
in the TEM literature.

The conventions about Miller indices notation in real space are
relatively clear. For example planes are denoted by round brackets
e.g. (100), directions by square brackets: [100], symmetrically
equivalent planes by curly brackets : {100}, and symmetrically
equivalent directions by angle brackets: {100} .

The situation becomes confusing when reciprocal space notations are
introduced.
The most widely accepted convention as far as I am aware is that
planes in reciprocal space or reflections are denoted with Miller
indices without brackets e.g. 100.
Directions are denoted with asterisk as superscript e.g. 100* but
also [100]* is used, so which one should be used? If it is [100]*
then denoting reflections without brackets is inconsistent, it should
be (100)*.
Using curly brackets for denoting planes/reflections in reciprocal
space is not proper, but how should symmetrically equivalent planes/
reflections in reciprocal space be denoted? Maybe by using asterisk
but if brackets are not proper for specific planes/reflections in
reciprocal space then why they should be proper for curly brackets?
Similar question arises for the notation of symmetrically equivalent
directions in reciprocal space.

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu




==============================Original Headers==============================
9, 22 -- From bozhilov-at-ucr.edu Sun Apr 12 18:19:46 2009
9, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3CNJjRf026159
9, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2009 18:19:46 -0500
9, 22 -- Received: from [192.168.100.104] (vpn-138-23-234-83.ucr.edu [138.23.234.83])
9, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
9, 22 -- with ESMTP id DPT77235 (AUTH bozhilov-at-ucr.edu)
9, 22 -- for {Microscopy-at-microscopy.com} ;
9, 22 -- Sun, 12 Apr 2009 16:19:45 -0700 (PDT)
9, 22 -- Message-Id: {87820979-F803-463D-AF2E-F6F989C56C59-at-ucr.edu}
9, 22 -- From: KN Bozhilov {bozhilov-at-ucr.edu}
9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 22 -- In-Reply-To: {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
9, 22 -- Subject: Miller indices
9, 22 -- X-Priority: 3
9, 22 -- Date: Sun, 12 Apr 2009 16:19:44 -0700
9, 22 -- References: {200805082342.m48NgkeG022691-at-ns.microscopy.com} {012701c8b269$5aa28650$b99fcb96-at-siap6segs20pa8} {457BCD4F-3FD9-4599-8363-2AF1A5775159-at-ucr.edu} {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
9, 22 -- X-Mailer: Apple Mail (2.930.3)
9, 22 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 13 Apr 2009 08:52:41 -0500
Subject: [Microscopy] Microscopy Society of Northeast Ohio spring meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy Society of Northeast Ohio (www.msneo.org) announces their
spring 09 meeting to be held at the Cleveland Museum of Art on May 6.

This meeting has been in the making for five years while the museum
underwent refurbishing. The museum now houses some of the most modern
conservation labs in the area.

The meeting starts at 4:00 with wine and cheese followed by a tour of the
labs and dinner. Our speaker will be Dean Yoder, Conservator of Paintings,
who will present “Conservation of Apollo and four Muses by the artist
Charles Meynier.â€

This is an unprecedented behind-the-scenes look at the Cleveland Museum of
Art and will be of interest to the microscopist, chemist and art lovers.
Comments and observations of the meeting are welcome at the MSNO blog at
http://www.msneo.org/newsletters.html.

RSVP to Pat Glazebrook no later than May 1, 2009. Pat can be reached at
pglazebrook-at-metrohealth.org or (216) 778-8958.

Cost: $25 - members, $35 - non-members,
$10 - student members, $15 - student non-members.


PAY ONLINE THROUGH PAYPAL AT: http://www.msneo.org/meetings.html

stay safe.........
Frank
--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
11, 23 -- From frank_karl-at-lincolnelectric.com Mon Apr 13 08:52:40 2009
11, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DDqeSX018039
11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 08:52:40 -0500
11, 23 -- Subject: Microscopy Society of Northeast Ohio spring meeting
11, 23 -- To: Microscopy-at-microscopy.com
11, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
11, 23 -- Message-ID: {OFF82F3AF9.521C923C-ON85257597.004C08B4-85257597.004C3247-at-lincolnelectric.com}
11, 23 -- Date: Mon, 13 Apr 2009 09:52:22 -0400
11, 23 -- From: Frank_Karl-at-lincolnelectric.com
11, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM,
11, 23 -- CD-MIME complete at 04/13/2009 09:52:24 AM,
11, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM,
11, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM,
11, 23 -- Serialize complete at 04/13/2009 09:52:24 AM
11, 23 -- MIME-Version: 1.0
11, 23 -- Content-Type: text/plain;
11, 23 -- charset="UTF-8"
11, 23 -- Content-Transfer-Encoding: 8bit
11, 23 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n3DDqeSX018039
==============================End of - Headers==============================




From: hyi-at-emory.edu
Date: Mon, 13 Apr 2009 13:09:15 -0500
Subject: [Microscopy] UC6rt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We routinely embed powders in epoxy and cut them normally. Usually it's
as simple as just putting a pinch of dry powder in the tip of a
microcentrifuge tube and filling it with resin. Make sure the powder is
really dry. Don't use a lot of powder----just a smidgen.

We generally cut sections that are 70-85 nm, but I don't see any reason
you can't go thinner. 20 might be stretching it, especially since some
particles can pop out of the sections, even in thicker slices.

Be very careful with your knife. If you're using diamond knives, test
the powder on an old, beat up one in case the particles nick the edge.
Or use glass.

Hope this helps.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: fwang-at-phys.ualberta.ca [mailto:fwang-at-phys.ualberta.ca]
Sent: Sunday, April 12, 2009 8:54 AM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both fwang-at-phys.ualberta.ca as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: fwang-at-phys.ualberta.ca
Name: Feng

Organization: Brookhaven National Lab

Title-Subject: [Filtered] TEM sample preparation by Microtome

Question: Hi all,

We hope to prepare cross-section TEM samples from micron-size powder
(like transition metal oxides), and wonder if we can embed this kind
of powder into epoxy and cut it into thin slices (such as 20-40nm in
thickness) by some special mirotome.

Since I don't have any experience with microtome for TEM sample
preparation, any suggestions on possiblities, requirement, tricks,
would be appreciated.

Also we may think of buying the facilities if such kind of work can
be done with microtome, please let me know if you have any related
informaiton, thank you.

Feng



Login Host: 130.199.3.140
------------------------------------------------------------------------
---

==============================Original
Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Sun Apr 12 08:52:57 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n3CDqt7s012053
12, 11 -- for {microscopy-at-microscopy.com} ; Sun, 12 Apr 2009
08:52:57 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c607a2a94a36-at-[206.69.208.22]}
12, 11 -- Date: Sun, 12 Apr 2009 08:52:55 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: fwang-at-phys.ualberta.ca (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: TEM sample preparation by Microtome
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
26, 30 -- From TindallR-at-missouri.edu Mon Apr 13 09:02:30 2009
26, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DE2T5R032163
26, 30 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 09:02:30 -0500
26, 30 -- X-IronPort-Anti-Spam-Filtered: true
26, 30 -- X-IronPort-Anti-Spam-Result: ApoEAIrh4knRauUo/2dsb2JhbAC+FQEBAQeETohNgkkBAYExBg
26, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
26, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 13 Apr 2009 09:02:29 -0500
26, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
26, 30 -- Mon, 13 Apr 2009 09:02:29 -0500
26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
26, 30 -- Content-class: urn:content-classes:message
26, 30 -- MIME-Version: 1.0
26, 30 -- Content-Type: text/plain;
26, 30 -- charset="us-ascii"
26, 30 -- Subject: RE: [Microscopy] viaWWW: TEM sample preparation by Microtome
26, 30 -- Date: Mon, 13 Apr 2009 09:01:29 -0500
26, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD802D-at-UM-XMAIL08.um.umsystem.edu}
26, 30 -- In-Reply-To: {200904121354.n3CDsS59013745-at-ns.microscopy.com}
26, 30 -- X-MS-Has-Attach:
26, 30 -- X-MS-TNEF-Correlator:
26, 30 -- Thread-Topic: [Microscopy] viaWWW: TEM sample preparation by Microtome
26, 30 -- Thread-Index: Acm7djMpkBarGKbsTdu0BKkt3RoFlgAyWQvQ
26, 30 -- References: {200904121354.n3CDsS59013745-at-ns.microscopy.com}
26, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
26, 30 -- To: {fwang-at-phys.ualberta.ca}
26, 30 -- Cc: {microscopy-at-microscopy.com}
26, 30 -- X-OriginalArrivalTime: 13 Apr 2009 14:02:29.0088 (UTC) FILETIME=[7B647600:01C9BC40]
26, 30 -- Content-Transfer-Encoding: 8bit
26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3DE2T5R032163
==============================End of - Headers==============================

From nbkjgdn-at-iahhgyaon.tpts4.seed.nz Mon Apr 13 10:12:03 2009
Return-Path: {nbkjgdn-at-iahhgyaon.tpts4.seed.nz}
Received: from google.com (133.sub-70-194-178.myvzw.com [70.194.178.133])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DFC2gp016303
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 13 Apr 2009 10:12:02 -0500
Received: from [153.90.98.144] (HELO google.com)
by cozyice.cn; Mon, 13 Apr 2009 11:12:02 -0400
Message-ID: {00000006CB38698766543101}
Reply-To: Roly Wiggins {16394spraginslorelle-at-gmail.com}

Dear All:

I would like to have your comments on the performance of Leica UC6rt
ultramicortome. This is the model dedicated to room temperature sectioning.
Please reply to me off-line. Thank you all very much in advance.

Hong


This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).


==============================Original Headers==============================
7, 32 -- From hyi-at-emory.edu Mon Apr 13 13:09:15 2009
7, 32 -- Received: from mr1.cc.emory.edu (mr1.cc.emory.edu [170.140.52.90])
7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DI9FmJ023693
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 13:09:15 -0500
7, 32 -- Received: from EXCHEDGE1.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
7, 32 -- by mr1.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n3DI8uth030951
7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 14:08:56 -0400
7, 32 -- Received: from EXCHHUB2.Enterprise.emory.net (170.140.30.54) by
7, 32 -- EXCHEDGE1.enterprise.emory.net (170.140.52.33) with Microsoft SMTP Server
7, 32 -- (TLS) id 8.1.358.0; Mon, 13 Apr 2009 14:08:52 -0400
7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
7, 32 -- EXCHHUB2.Enterprise.emory.net ([170.140.30.54]) with mapi; Mon, 13 Apr 2009
7, 32 -- 14:08:55 -0400
7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu}
7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
7, 32 -- Date: Mon, 13 Apr 2009 14:08:54 -0400
7, 32 -- Subject: UC6rt
7, 32 -- Thread-Topic: UC6rt
7, 32 -- Thread-Index: Acm8YufioZrv+3a5A0O5WcYrgzVQBw==
7, 32 -- Message-ID: {C608F7F6.AC00%hyi-at-emory.edu}
7, 32 -- Accept-Language: en-US
7, 32 -- Content-Language: en
7, 32 -- X-MS-Has-Attach:
7, 32 -- X-MS-TNEF-Correlator:
7, 32 -- acceptlanguage: en-US
7, 32 -- Content-Type: text/plain; charset="iso-8859-1"
7, 32 -- MIME-Version: 1.0
7, 32 -- X-emory.edu-MailScanner: Found to be clean
7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
7, 32 -- X-Spam-Status: No
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3DI9FmJ023693
==============================End of - Headers==============================




From: ALawrence-at-entomology.msstate.edu
Date: Mon, 13 Apr 2009 16:47:16 -0500
Subject: [Microscopy] M&M 2009 student bursaries and volunteers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

The M&M 2009 program is set, sessions are scheduled, and the count down
to the Richmond meeting is on.

I encourage all students, or those of you who have students, attending
the meeting to please consider the bursary program sponsored by the MSA
education committee. The purpose of this program is to encourage
students to attend the annual MSA/MAS Microscopy and Microanalysis
meeting, where they can meet and interact with the established
microscopy community while defraying some meeting costs.

The students work for approximately 20 hours (or up to 40 hours) during
the meeting and pre-meeting events and are paid $10 an hour. The jobs
involve such things as providing support in the different symposia
(helping with audio-visual needs, maintaining an attendance count, and
helping speakers set up for their presentation), staffing the MSA
Megabooth or volunteer office, monitoring use of the Internet Café, and
helping with poster set-up and take-down.

Each participant will be sent the task list where they can sign-up for
the jobs and times they want. In most instances students end up
*working* sessions they would attend anyway. There is an added
bonus of a $10 cash meal allotment for each morning and/or afternoon
session worked.

For those *non-students* we could always use volunteers to help
fill-in with the above mentioned meeting activities as well. Although
not paid on an hourly basis as the student bursaries, volunteers do
receive some compensation along with the same cash allotment for meals.
They also have the added benefit of getting to interact more with the
microscopy community as they assist with meeting tasks.

Those interested in the bursary program should check the *I wish to
apply for a student bursary* box in section 2 of the registration form
(must be members of MSA or MAS, and enrolled as students at a recognized
educational institution).

If anyone has any questions about the bursary/volunteer program, or
would like to participate, please contact me (off-line).

Amanda Lawrence
Electron Microscope Center
Mississippi State University
662-325-3019
alawrence-at-entomology.msstate.edu




==============================Original Headers==============================
12, 20 -- From ALawrence-at-entomology.msstate.edu Mon Apr 13 16:47:16 2009
12, 20 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DLlGEN012316
12, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 16:47:16 -0500
12, 20 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197])
12, 20 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n3DLlFZu009186
12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL)
12, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 16:47:15 -0500
12, 20 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu
12, 20 -- with Novell_GroupWise; Mon, 13 Apr 2009 16:47:15 -0500
12, 20 -- Message-Id: {49E36C8D.B26A.00E6.0-at-entomology.msstate.edu}
12, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
12, 20 -- Date: Mon, 13 Apr 2009 16:47:09 -0500
12, 20 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu}
12, 20 -- To: {microscopy-at-microscopy.com}
12, 20 -- Subject: M&M 2009 student bursaries and volunteers
12, 20 -- Mime-Version: 1.0
12, 20 -- Content-Type: text/plain; charset=ISO-8859-15
12, 20 -- Content-Transfer-Encoding: 8bit
12, 20 -- Content-Disposition: inline
==============================End of - Headers==============================




From: kamlennon-at-yahoo.com
Date: Mon, 13 Apr 2009 17:14:01 -0500
Subject: [Microscopy] Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I'm getting ready to "teach" my undergraduate EM class how to create a poster presentation of their work and I need a little educating as well. Could you please send me your sage advice on creating a poster in PowerPoint (that's what we are required to use) regarding the resolution of the electron micrographs? As background, we are developing TEM film, scanning the negatives, and manipulating them with Adobe Photoshop.

Thanks,
Kristen

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
Frostburg State University

kalennon-at-frostburg.edu




==============================Original Headers==============================
8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
8, 20 -- Received: from web84001.mail.mud.yahoo.com (web84001.mail.mud.yahoo.com [68.142.206.171])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3DME1fS026918
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01 -0500
8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 22:14:01 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqzv0F9Du06x80gL9WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99ILd8f0frdvPOc7pH66pXM=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2mHCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx7b+Mpe2yAzlxL11rki3h2FKA/pqZL+/La5Y=;
8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG: GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3YCQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbxci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQtDwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
8, 20 -- Received: from [96.239.150.112] by web84001.mail.mud.yahoo.com via HTTP; Mon, 13 Apr 2009 15:14:00 PDT
8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
8, 20 -- Subject: Advice: Resolution of micrographs for posters?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Tue, 14 Apr 2009 10:58:49 -0500
Subject: [Microscopy] Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI Kristen
There is lots to recommend Power point but posters isn't its strongest
suite.
Our media specialist tells me the Power Point's output size is one of its
weak points
Poster size width is determined by the printer being used (our is 43 inches)
while the maximum length is 56 inches
Lengths greater than 56 leave the printer scratching its head though there
are work arounds.

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca


-----Original Message-----
X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com]
Sent: Monday, April 13, 2009 6:19 PM
To: rjharris-at-uwo.ca


Hi All,

I'm getting ready to "teach" my undergraduate EM class how to create a
poster presentation of their work and I need a little educating as well.
Could you please send me your sage advice on creating a poster in PowerPoint
(that's what we are required to use) regarding the resolution of the
electron micrographs? As background, we are developing TEM film, scanning
the negatives, and manipulating them with Adobe Photoshop.

Thanks,
Kristen

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
Frostburg State University

kalennon-at-frostburg.edu




==============================Original Headers==============================
8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
8, 20 -- Received: from web84001.mail.mud.yahoo.com
(web84001.mail.mud.yahoo.com [68.142.206.171])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n3DME1fS026918
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01
-0500
8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 22:14:01
-0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqzv0F9Du06x80gL9
WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99
ILd8f0frdvPOc7pH66pXM=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
8, 20 --
b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2m
HCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx7b+Mpe2yAzlxL1
1rki3h2FKA/pqZL+/La5Y=;
8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG:
GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4
WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3Y
CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbx
ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQt
DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
8, 20 -- Received: from [96.239.150.112] by web84001.mail.mud.yahoo.com via
HTTP; Mon, 13 Apr 2009 15:14:00 PDT
8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
8, 20 -- Subject: Advice: Resolution of micrographs for posters?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================



==============================Original Headers==============================
19, 29 -- From rjharris-at-uwo.ca Tue Apr 14 10:58:49 2009
19, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EFwmH3008207
19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 10:58:49 -0500
19, 29 -- MIME-version: 1.0
19, 29 -- Content-type: text/plain; charset=iso-8859-1
19, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
19, 29 -- with ESMTP id {0KI300L1FLPZ0Y00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
19, 29 -- with ESMTPSA id {0KI300K3ZLPZEI00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
19, 29 -- To: kamlennon-at-yahoo.com
19, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
19, 29 -- References: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- In-reply-to: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- Subject: RE: [Microscopy] Advice: Resolution of micrographs for posters?
19, 29 -- Date: Tue, 14 Apr 2009 11:58:47 -0400
19, 29 -- Message-id: {00f601c9bd19$e5888010$b0998030$-at-ca}
19, 29 -- X-Mailer: Microsoft Office Outlook 12.0
19, 29 -- Thread-index: Acm8hexsPjIfYHJqSt6Tn+qeWbWeggAk10Nw
19, 29 -- Content-language: en-us
19, 29 -- Content-Transfer-Encoding: 8bit
19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3EFwmH3008207
==============================End of - Headers==============================




From: ALawrence-at-entomology.msstate.edu
Date: Tue, 14 Apr 2009 12:52:46 -0500
Subject: [Microscopy] Southeastern Microscopy annual meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Malcolm,
Now that I think about it, one of the trickiest variables I've observed when immunolabeling is the high degree of variability of antigen robustness. Some antibodies show consistent immunostaining patterns for years on sectioned paraffin slides, and are not sensitive to fixation variables. Others, especially recent proliferation protein antibodies and some other "high tech" antibodies, show high immunostaining variability depending on: 1) the fixative type, 2) the timing of fixation (sensitivity to over and underfixation), and 3) the tissue section age (as queried in this original topic). Yet another variable is the introduction and refinement of new antigen retrieval/unmasking techniques over the past fifteen years or so.

Yes, the antibodies do not penetrate beyond the cut surface of the resin, unless the resin is reduced with sodium ethaoxide. I agree with you that conserving the few antigen binding sites exposed on the EM section should be first priority, which is why I assume most people use fresh sections.

I haven't been able to find any publications to share at this time. My apologies for the delayed response, as I mistakenly thought I'd have time to perform a literature search.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: Malcolm Haswell [mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Wednesday, April 08, 2009 12:16 PM
To: Sobocinski, Gregg; Microscopy MSA

We would like to invite all to attend the Southeastern Microscopy
Society (SEMS) 09 meeting to be held in Athens, GA May 27-29. SEMS is a
great venue for networking with others in microscopy/ microanalysis
related fields, developing new collaborations, learning about
state-of-the-art tools for microscopy/microanalysis from vendors, and
catching up with friends (www.southeasternmicrosocpy.org) .

Invited speakers for this year*s meeting are: Dr. Sara Miller, Duke
University; Dr. Jay Jerome, Vanderbilt University; Dr. Wilma Lingle,
Mayo Clinic, Rochester, MN; and Dr. Yiping Zhao, University of Georgia.
Short courses include: RMC Cryo Sectioning, EMS QuantomiX, Leica Live
Cell Confocal Multi Photon Imaging, Hitachi Tabletop SEM TM-1000 Demo,
and a PhotoShop Workshop hosted by Dr. Jay Jerome. There will also be
numerous platform and poster presentations as well as vendor talks and
demos.

We encourage all to register for the SEMS meeting and perhaps consider
contributing to the meeting by way of platform or poster presentation.
You can go to http://southeasternmicroscopy.org/index-2.htmlto register
and find forms for abstract submission (call for papers; deadline has
been extended to April 17), technician travel award, Ruska Award, and
photo contest entry. A preliminary program can be found in the brochure
online. An undated program will be posted to this site soon. You can
also go to
http://www.georgiacenter.uga.edu/conferences/2009/May/27/sms.phtml to
register for SEMS directly.

Please pass the word to collaborators, faculty, staff and students and
join us at SEMS 09.
I look forward to seeing you in Athens next month.

cheers, Giselle

SEMS President

Giselle Thibaudeau, Director Electron Microscope Center
Associate Professor of Biological Sciences
Mississippi State University
phone 662-325-3017
email giselle-at-emcenter.msstate.edu



==============================Original Headers==============================
9, 20 -- From ALawrence-at-entomology.msstate.edu Tue Apr 14 12:52:46 2009
9, 20 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EHqjC9008529
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 12:52:46 -0500
9, 20 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197])
9, 20 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n3EHqj1Q015870
9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL)
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 12:52:45 -0500
9, 20 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu
9, 20 -- with Novell_GroupWise; Tue, 14 Apr 2009 12:52:45 -0500
9, 20 -- Message-Id: {49E4871B.B26A.00E6.0-at-entomology.msstate.edu}
9, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
9, 20 -- Date: Tue, 14 Apr 2009 12:52:44 -0500
9, 20 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu}
9, 20 -- To: {microscopy-at-microscopy.com}
9, 20 -- Subject: Southeastern Microscopy annual meeting
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset=ISO-8859-15
9, 20 -- Content-Transfer-Encoding: 8bit
9, 20 -- Content-Disposition: inline
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Tue, 14 Apr 2009 12:56:32 -0500
Subject: [Microscopy] Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just took my 'slides' to Kinko's and had them printed up to desired size.
Worked great.

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.



Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA

TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com



-----Original Message-----
X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
Sent: Tuesday, April 14, 2009 9:11 AM
To: dkloos-at-parallaxray.com

HI Kristen
There is lots to recommend Power point but posters isn't its strongest
suite.
Our media specialist tells me the Power Point's output size is one of its
weak points
Poster size width is determined by the printer being used (our is 43 inches)
while the maximum length is 56 inches
Lengths greater than 56 leave the printer scratching its head though there
are work arounds.

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca


-----Original Message-----
X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com]
Sent: Monday, April 13, 2009 6:19 PM
To: rjharris-at-uwo.ca


Hi All,

I'm getting ready to "teach" my undergraduate EM class how to create a
poster presentation of their work and I need a little educating as well.
Could you please send me your sage advice on creating a poster in PowerPoint
(that's what we are required to use) regarding the resolution of the
electron micrographs? As background, we are developing TEM film, scanning
the negatives, and manipulating them with Adobe Photoshop.

Thanks,
Kristen

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
Frostburg State University

kalennon-at-frostburg.edu




==============================Original Headers==============================
8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
8, 20 -- Received: from web84001.mail.mud.yahoo.com
(web84001.mail.mud.yahoo.com [68.142.206.171])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n3DME1fS026918
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01
-0500
8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 22:14:01
-0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqzv0F9Du06x80gL9
WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99
ILd8f0frdvPOc7pH66pXM=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
8, 20 --
b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2m
HCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx7b+Mpe2yAzlxL1
1rki3h2FKA/pqZL+/La5Y=;
8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG:
GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4
WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3Y
CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbx
ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQt
DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
8, 20 -- Received: from [96.239.150.112] by web84001.mail.mud.yahoo.com via
HTTP; Mon, 13 Apr 2009 15:14:00 PDT
8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
8, 20 -- Subject: Advice: Resolution of micrographs for posters?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================



==============================Original Headers==============================
19, 29 -- From rjharris-at-uwo.ca Tue Apr 14 10:58:49 2009
19, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3EFwmH3008207
19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 10:58:49
-0500
19, 29 -- MIME-version: 1.0
19, 29 -- Content-type: text/plain; charset=iso-8859-1
19, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri
[172.29.32.39])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26
2008; 32bit))
19, 29 -- with ESMTP id {0KI300L1FLPZ0Y00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26
2008; 32bit))
19, 29 -- with ESMTPSA id {0KI300K3ZLPZEI00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
19, 29 -- To: kamlennon-at-yahoo.com
19, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
19, 29 -- References: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- In-reply-to: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- Subject: RE: [Microscopy] Advice: Resolution of micrographs for
posters?
19, 29 -- Date: Tue, 14 Apr 2009 11:58:47 -0400
19, 29 -- Message-id: {00f601c9bd19$e5888010$b0998030$-at-ca}
19, 29 -- X-Mailer: Microsoft Office Outlook 12.0
19, 29 -- Thread-index: Acm8hexsPjIfYHJqSt6Tn+qeWbWeggAk10Nw
19, 29 -- Content-language: en-us
19, 29 -- Content-Transfer-Encoding: 8bit
19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3EFwmH3008207
==============================End of - Headers==============================



==============================Original Headers==============================
29, 31 -- From dkloos-at-parallaxray.com Tue Apr 14 12:56:32 2009
29, 31 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
29, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EHuVqD014372
29, 31 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 12:56:31 -0500
29, 31 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
29, 31 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
29, 31 -- (envelope-from {dkloos-at-parallaxray.com} )
29, 31 -- id 1Ltms8-00034B-Nl; Tue, 14 Apr 2009 11:56:36 -0600
29, 31 -- Reply-To: {dkloos-at-parallaxray.com}
29, 31 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
29, 31 -- To: {microscopy-at-microscopy.com}
29, 31 -- Cc: {rjharris-at-uwo.ca}
29, 31 -- References: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
29, 31 -- Subject: RE: [Microscopy] RE: Advice: Resolution of micrographs for posters?
29, 31 -- Date: Tue, 14 Apr 2009 10:56:24 -0700
29, 31 -- Organization: Parallax Research
29, 31 -- Message-ID: {2B5B9B340BD2412FB23D2C53AF29F19E-at-donl}
29, 31 -- MIME-Version: 1.0
29, 31 -- Content-Type: text/plain;
29, 31 -- charset="iso-8859-1"
29, 31 -- X-Mailer: Microsoft Office Outlook 11
29, 31 -- In-Reply-To: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
29, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
29, 31 -- Thread-Index: Acm9G64h6c8cFDLwQ/+utZ/f7mH7TwADom1A
29, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
29, 31 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
29, 31 -- X-AntiAbuse: Original Domain - microscopy.com
29, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
29, 31 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
29, 31 -- Content-Transfer-Encoding: 8bit
29, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3EHuVqD014372
==============================End of - Headers==============================




From: lisa-at-glosuntech.com
Date: Tue, 14 Apr 2009 15:04:54 -0500
Subject: [Microscopy] TEM Disassemble service needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listserver members,


We need to disassemble and pack a used Jeol JEM 200CX TEM in San Francisco area (No reassembling service needed at this time) so that we can load it to a truck for moving. To reduce our cost, our company will provide you with an assistant if two people's work is needed during the disassembling process. The working time is flexible and you may work during weekend.


If you are interested, please submit your bid with your information to lisa-at-glosuntech.com. Contract will be given to the one with the lowest bid price.

Thank you!


Regards,


Lisa

Glosuntech LLC

==============================Original Headers==============================
7, 31 -- From lisa-at-glosuntech.com Tue Apr 14 15:04:54 2009
7, 31 -- Received: from mta4.brinkster.com (mta4.brinkster.com [65.182.109.73])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EK4scC008778
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 15:04:54 -0500
7, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 6DE3C5EDE0B
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 16:04:51 -0400 (EDT)
7, 31 -- X-Virus-Scanned: amavisd-new at
7, 31 -- X-Spam-Flag: NO
7, 31 -- X-Spam-Score: -2.499
7, 31 -- X-Spam-Level:
7, 31 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=5
7, 31 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1]
7, 31 -- Received: from mta4.brinkster.com ([127.0.0.1])
7, 31 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024)
7, 31 -- with ESMTP id UItkXdwH4OFH for {Microscopy-at-Microscopy.Com} ;
7, 31 -- Tue, 14 Apr 2009 16:04:46 -0400 (EDT)
7, 31 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217])
7, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id DDFBC5EDE75
7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 16:04:45 -0400 (EDT)
7, 31 -- Date: Tue, 14 Apr 2009 16:04:47 -0400 (EDT)
7, 31 -- From: lisa-at-glosuntech.com
7, 31 -- To: Microscopy-at-Microscopy.Com
7, 31 -- Message-ID: {29065733.31591239739487398.JavaMail.root-at-mail10a.brinkster.com}
7, 31 -- In-Reply-To: {31101946.31551239739412083.JavaMail.root-at-mail10a.brinkster.com}
7, 31 -- Subject: TEM Disassemble service needed
7, 31 -- MIME-Version: 1.0
7, 31 -- Content-Type: text/plain; charset=utf-8
7, 31 -- Content-Transfer-Encoding: 7bit
7, 31 -- X-Originating-IP: [75.140.108.220]
7, 31 -- X-Mailer: Zimbra 5.0.8_GA_2463.RHEL4 (ZimbraWebClient - FF3.0 (Win)/5.0.8_GA_2462.RHEL4_64)
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Tue, 14 Apr 2009 15:38:28 -0500
Subject: [Microscopy] Fwd: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kirsten,

You and your students will be fine with PowerPoint. For posters larger
than what PowerPoint's setup allows, we scale a little down, keeping
the desired proportion, then we increase the size (e.g., 123%) during
printing.

I have been using PP for posters since 2000, with different printers.
Until 2-3 years ago, we would have to go with our files to a
centralized printing facility, but now we have our own large HP
printer, and even new users have little problem using it from their
desktops. I've been meaning to switch to Adobe InDesign for posters
for years, but guess what, whenever the time comes to make a new
poster, there is always a hurry, and never enough time to learn new
software! I am neither lover or hater of Microsoft products, use them
as a tool, and PPoint has been very convenient for us.

I recommend making all adjustments to your photos while still in
Photoshop, and all labels, lettering, and scale in PowerPoint. This
way it'll be easier to do late adjustments like font size. We switch
PS color management off and try to achieve the desired gray levels on
all images. Once everything is to our liking, we reduce the pixel size
and save under different name.

Now, to your particular question - we aim for 150-200 pixels per inch
in the resulting full-size poster print. That's from some early
testing we did. We set picture size in PS, in inches, to what it
approximately is going to be in the final print and then set
resolution to 150-200 ppi. Then save the new image separately as
TIFF, that's the best and should not cause any file size issue these
days. Then we insert (or drag-n-drop) those saved images onto the
poster in PPoint. It is very common to have to resize the image a bit
this or that way before the poster is finished, that's why the ppi
range. 175 ppi is a good starting number. You'll get a feeling after a
few posters. Don't be afraid to explore lower ppi - as long as you see
all relevant features, of course, images printed from lower ppi tend
to look better than oversampled (too high ppi) images on a poster.
Below 150 ppi in the final print, you may notice the reduced
resolution, but it won't look bad. As long as you use TIFFs,
PowerPoint will do nice smoothening, and there'll be no pixelation.

Good luck.
Please don't hesitate to contact me directly if I wasn't clear,
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com}
} Date: April 13, 2009 6:14:52 PM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
} Reply-To: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Hi All,
}
} I'm getting ready to "teach" my undergraduate EM class how to create
} a poster presentation of their work and I need a little educating as
} well. Could you please send me your sage advice on creating a poster
} in PowerPoint (that's what we are required to use) regarding the
} resolution of the electron micrographs? As background, we are
} developing TEM film, scanning the negatives, and manipulating them
} with Adobe Photoshop.
}
} Thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} Frostburg State University
}
} kalennon-at-frostburg.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
} 8, 20 -- Received: from web84001.mail.mud.yahoo.com
} (web84001.mail.mud.yahoo.com [68.142.206.171])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} id n3DME1fS026918
} 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01
} -0500
} 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009
} 22:14:01 -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} d=yahoo.com; s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr
} +0Nro1sSEP2MofzkAUoG+LFo=; h=Message-ID:X-YMail-OSG:Received:X-
} Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QdDLdklkKjl/
} vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/
} 5pfJcQHaRTqzv0F9Du06x80gL9WfK9tfgY5blF/
} Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99ILd8f0frdvPOc7pH66pXM
} =
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-
} Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
} 8, 20 -- b=CRJXqaYio8A65tWLDZzLUty0rsYt37M
} +
} 1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2mHCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt
} +9ZB+ajEXgx7b+Mpe2yAzlxL11rki3h2FKA/pqZL+/La5Y=;
} 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
} 8, 20 -- X-YMail-OSG:
} GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3YCQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbxci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQtDwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
} 8, 20 -- Received: from [96.239.150.112] by
} web84001.mail.mud.yahoo.com via HTTP; Mon, 13 Apr 2009 15:14:00 PDT
} 8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/
} 0.7.289.1
} 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
} 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 8, 20 -- Subject: Advice: Resolution of micrographs for posters?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
10, 23 -- From vladislav_speransky-at-nih.gov Tue Apr 14 15:38:28 2009
10, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EKcR9X023807
10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 15:38:27 -0500
10, 23 -- X-IronPortListener: NIH_Relay
10, 23 -- X-SBRS: None
10, 23 -- X-IronPort-AV: E=Sophos;i="4.40,186,1238990400";
10, 23 -- d="scan'208";a="83849612"
10, 23 -- Received: from helix.nih.gov ([128.231.2.3])
10, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 14 Apr 2009 16:38:27 -0400
10, 23 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185])
10, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3EKcRYj014498
10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 16:38:27 -0400
10, 23 -- Message-Id: {FD8A426D-3157-4DE4-8CB3-74D5112051DD-at-nih.gov}
10, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
10, 23 -- To: Microscopy-at-microscopy.com
10, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 23 -- Content-Transfer-Encoding: 7bit
10, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
10, 23 -- Subject: Fwd: [Microscopy] Advice: Resolution of micrographs for posters?
10, 23 -- Date: Tue, 14 Apr 2009 16:38:27 -0400
10, 23 -- References: {D13763A4740B07428566B25D7E768DA69F1C1A49-at-NIHMLBX02.nih.gov}
10, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: lanqiang76-at-yahoo.com
Date: Tue, 14 Apr 2009 19:30:07 -0500
Subject: [Microscopy] viaWWW: Manual for Reichert-Jung FC4E microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lanqiang76-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lanqiang76-at-yahoo.com
Name: Shawn Lan

Organization: Huntsman

Title-Subject: [Filtered] Manual for Reichert-Jung FC4E microtome

Question: We lost our manual for Reichert FC4E cryo-microtome
associated with Reichert-Jung Ultracut E. I called Leica and they do
not have the manual any longer becasue the instrument is too old. I
am wondering if anybody can help with a copy of the instruction
manual. We would like to pay any related copy and mail expenses.
Really appreciate the help. Thanks.


Login Host: 205.235.104.4
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Apr 14 19:30:07 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F0U7hl005169
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 19:30:07 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c60adaf23ccf-at-[206.69.208.22]}
7, 11 -- Date: Tue, 14 Apr 2009 19:30:06 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: lanqiang76-at-yahoo.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Manual for Reichert-Jung FC4E microtome
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ars-at-advressys.com
Date: Tue, 14 Apr 2009 23:32:48 -0500
Subject: [Microscopy] schematics for Leo 435 VP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Have a customer who, during an interdepartmental move, managed to lose the
schematics for their Leo 435VP. Can anyone provide a copy or scan? I'd be
happy to reimburse for any expense. Any info on hardware / software upgrades
would also be welcome (haven't contacted manufacturer yet, but will). It is
currently running Windows 3.1 and has a motherboard with 2 ISA slots that
aren't being used and a couple of PCI slots that are. Not sure if there
have been any upgrades from the original configuration.

*** Note new phone numbers, email & web addresses
Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois  60174
phone (800) 506-9770  fax (800) 506-9771
email ars-at-advressys.com  web www.advressys.com




==============================Original Headers==============================
5, 25 -- From ars-at-advressys.com Tue Apr 14 23:32:48 2009
5, 25 -- Received: from omr9.networksolutionsemail.com (omr9.networksolutionsemail.com [205.178.146.59])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F4WliT008610
5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 23:32:47 -0500
5, 25 -- Received: from mail.networksolutionsemail.com (ns-omr9.mgt.netsol.com [10.49.6.72])
5, 25 -- by omr9.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n3F4Wl5e016342
5, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 00:32:47 -0400
5, 25 -- Received: (qmail 30622 invoked by uid 78); 15 Apr 2009 04:32:47 -0000
5, 25 -- Received: from unknown (HELO vaiod) (ars-at-advressys.com-at-67.162.79.36)
5, 25 -- by ns-omr9.lb.hosting.dc2.netsol.com with SMTP; 15 Apr 2009 04:32:47 -0000
5, 25 -- Reply-To: {ars-at-advressys.com}
5, 25 -- From: "Allen R. Sampson" {ars-at-advressys.com}
5, 25 -- To: "'Microscopy Listserver'" {Microscopy-at-microscopy.com}
5, 25 -- Subject: schematics for Leo 435 VP
5, 25 -- Date: Tue, 14 Apr 2009 23:32:50 -0500
5, 25 -- Organization: Advanced Research Systems
5, 25 -- Message-ID: {77565B653BEF4654AC57291948FD9EB8-at-vaiod}
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Type: text/plain;
5, 25 -- charset="iso-8859-1"
5, 25 -- X-Mailer: Microsoft Office Outlook 11
5, 25 -- Thread-Index: Acm9gzuypxIpUXL1Tt+Ppna8WxBsgQ==
5, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
5, 25 -- Content-Transfer-Encoding: 8bit
5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3F4WliT008610
==============================End of - Headers==============================




From: nyilmaz-at-mersin.edu.tr
Date: Wed, 15 Apr 2009 04:03:57 -0500
Subject: [Microscopy] Antibody for ZP Proteins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We're planning to study ZP1, ZP2 and ZP3 protein immunohistochemistry on
mice ovary. We couldn't find commercially available antibodies for these
proteins suitable for IHC. Does anybody know sources for buying this items?
Thanks in advance...

Dr. Necat Yilmaz MD, PhD
University of Mersin


==============================Original Headers==============================
4, 31 -- From nyilmaz-at-mersin.edu.tr Wed Apr 15 04:03:57 2009
4, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F93tbj030441
4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 04:03:56 -0500
4, 31 -- Received: from localhost (localhost [127.0.0.1])
4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id DAEA72F710A
4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 12:03:14 +0300 (EEST)
4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr
4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1])
4, 31 -- by localhost (mail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024)
4, 31 -- with ESMTP id U5v-lDVb1+ru for {Microscopy-at-microscopy.com} ;
4, 31 -- Wed, 15 Apr 2009 12:02:26 +0300 (EEST)
4, 31 -- Received: from nejat1 (unknown [193.255.129.131])
4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 2C6EE2F7110
4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 12:00:02 +0300 (EEST)
4, 31 -- Message-ID: {000b01c9bda8$a6899680$2101a8c0-at-nejat1}
4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com}
4, 31 -- Subject: Antibody for ZP Proteins
4, 31 -- Date: Wed, 15 Apr 2009 12:00:40 +0300
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- format=flowed;
4, 31 -- charset="iso-8859-9";
4, 31 -- reply-type=response
4, 31 -- Content-Transfer-Encoding: 7bit
4, 31 -- X-Priority: 3
4, 31 -- X-MSMail-Priority: Normal
4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138
4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Wed, 15 Apr 2009 04:45:35 -0500
Subject: [Microscopy] Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Generally I use PowerPoint to produce my posters, which is fine as my
predecessor created our Core poster in PowerPoint and we never go above
poster size A0 [paper size 3 feet x 4 feet]. I have converted a few to MS
Publisher though. Other applications can be used but it should be noted that
Word will not accept paper size as big as A0. Our University printers do the
printing, we just provide the Excel ppt or Publisher pub file.

Image size is kind of irrelevant provided your image resolution is at least
600 pixels per inch on the paper [it can be higher res in the document]. You
can often get away with less if that’s all you have [upscaling in Photoshop
can help], as often it's only the University/Centre logos that look really
naff and pixellated at low-res. Generally it's useful to make all the images
quite high resolution, say 1000 pixels per inch, as I quite often nick
images from our poster, say for the website, and it's a pain having to track
down the original.

You do need Photoshop or similar [Elements or Serif PhotoPlus] to
edit/crop/enhance images. Large Powerpoint sizes [Mb file size] due to high
resolution graphics isn't a problem for a modern PC [well for mine anyway as
it is an imaging workstation and gaming powerhouse], plus it simply goes off
to the printers on a CD or RAM drive. Don't use 6,400 dpi scanned TEM ones
though unless you are a patient sort on the PC, downscale to or scanning at
1,200 dpi seems fine for most applications [unless of course you are
printing that single TEM film image at size A0]. Going below 300 dpi on the
printed page might look bad, but it depends a lot on the image itself. For
pdf's use Adobes Acrobat Pro's pdf optimiser and generally output for the
latest acrobat version compatibility. Our OUCS printers seem to prefer
output from ubiquitous PowerPoint or Publisher [word is limited to 22 inches
size I believe].

The advantage of Powerpoint is that everyone has it, and it works well at
least up to the standard A0 poster size. Microsoft Publisher is probably a
better option but it's less common and not MS Office's greatest app [Serif's
PagePlus is better and a third of the price]. Plus there's Adobe's Indesign
and Quark express for those with deep pockets [probably not students].

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
Sent: 14 April 2009 17:09
To: kjmorris-at-well.ox.ac.uk

HI Kristen
There is lots to recommend Power point but posters isn't its strongest
suite.
Our media specialist tells me the Power Point's output size is one of its
weak points
Poster size width is determined by the printer being used (our is 43 inches)
while the maximum length is 56 inches
Lengths greater than 56 leave the printer scratching its head though there
are work arounds.

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca


-----Original Message-----
X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com]
Sent: Monday, April 13, 2009 6:19 PM
To: rjharris-at-uwo.ca


Hi All,

I'm getting ready to "teach" my undergraduate EM class how to create a
poster presentation of their work and I need a little educating as well.
Could you please send me your sage advice on creating a poster in PowerPoint
(that's what we are required to use) regarding the resolution of the
electron micrographs? As background, we are developing TEM film, scanning
the negatives, and manipulating them with Adobe Photoshop.

Thanks,
Kristen

Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
Frostburg State University

kalennon-at-frostburg.edu




==============================Original Headers==============================
8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
8, 20 -- Received: from web84001.mail.mud.yahoo.com
(web84001.mail.mud.yahoo.com [68.142.206.171])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n3DME1fS026918
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01
-0500
8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 22:14:01
-0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqzv0F9Du06x80gL9
WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99
ILd8f0frdvPOc7pH66pXM=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
8, 20 --
b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2m
HCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx7b+Mpe2yAzlxL1
1rki3h2FKA/pqZL+/La5Y=;
8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG:
GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4
WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3Y
CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbx
ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQt
DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
8, 20 -- Received: from [96.239.150.112] by web84001.mail.mud.yahoo.com via
HTTP; Mon, 13 Apr 2009 15:14:00 PDT
8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1
8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
8, 20 -- Subject: Advice: Resolution of micrographs for posters?
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================



==============================Original Headers==============================
19, 29 -- From rjharris-at-uwo.ca Tue Apr 14 10:58:49 2009
19, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3EFwmH3008207
19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 10:58:49
-0500
19, 29 -- MIME-version: 1.0
19, 29 -- Content-type: text/plain; charset=iso-8859-1
19, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri
[172.29.32.39])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26
2008; 32bit))
19, 29 -- with ESMTP id {0KI300L1FLPZ0Y00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17])
19, 29 -- by harpo.mail.uwo.pri
19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26
2008; 32bit))
19, 29 -- with ESMTPSA id {0KI300K3ZLPZEI00-at-harpo.mail.uwo.pri} for
19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009 11:58:47 -0400 (EDT)
19, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
19, 29 -- To: kamlennon-at-yahoo.com
19, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
19, 29 -- References: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- In-reply-to: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
19, 29 -- Subject: RE: [Microscopy] Advice: Resolution of micrographs for
posters?
19, 29 -- Date: Tue, 14 Apr 2009 11:58:47 -0400
19, 29 -- Message-id: {00f601c9bd19$e5888010$b0998030$-at-ca}
19, 29 -- X-Mailer: Microsoft Office Outlook 12.0
19, 29 -- Thread-index: Acm8hexsPjIfYHJqSt6Tn+qeWbWeggAk10Nw
19, 29 -- Content-language: en-us
19, 29 -- Content-Transfer-Encoding: 8bit
19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3EFwmH3008207
==============================End of - Headers==============================



==============================Original Headers==============================
33, 23 -- From kjmorris-at-well.ox.ac.uk Wed Apr 15 04:45:35 2009
33, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
33, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F9jYKv013334
33, 23 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 04:45:34 -0500
33, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
33, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
33, 23 -- id 1Lu1gT-0003oN-QO
33, 23 -- for microscopy-at-microscopy.com; Wed, 15 Apr 2009 10:45:33 +0100
33, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
33, 23 -- To: {microscopy-at-microscopy.com}
33, 23 -- References: {200904141609.n3EG9TLO018765-at-ns.microscopy.com}
33, 23 -- Subject: RE: [Microscopy] RE: Advice: Resolution of micrographs for posters?
33, 23 -- Date: Wed, 15 Apr 2009 10:45:33 +0100
33, 23 -- Message-ID: {6844F4522FE44DE091AD2E1B7E626BE1-at-CytoWhizz}
33, 23 -- MIME-Version: 1.0
33, 23 -- Content-Type: text/plain;
33, 23 -- charset="iso-8859-1"
33, 23 -- X-Mailer: Microsoft Office Outlook 11
33, 23 -- Thread-Index: Acm9G2Q5gF+h2s10R+Cc9cUyNLzb8gAjE5wg
33, 23 -- In-Reply-To: {200904141609.n3EG9TLO018765-at-ns.microscopy.com}
33, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
33, 23 -- Content-Transfer-Encoding: 8bit
33, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3F9jYKv013334
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Wed, 15 Apr 2009 06:26:19 -0500
Subject: [Microscopy] Fwd: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Vlad,

Sorry didn't see your posting - all good advice. As we also produce our
posters for pdf [where the viewer can zoom] we aim for higher dpi in the
original ppt file [doesn't do any harm] - plus I do like to nick them for
other uses. I guess many of our images are generally lower than 600 dpi on
the actual page, typically 300 dpi, and as you say they still look fine.
Nothing wrong with higher resolution though.

Once scaled down on-screen PowerPoint doesn't seem to mind our 'hi-res'
images though [at 600 dpi], in terms of PC response when moving around the
presentation.

With any image editing/graphics design more PC memory helps a lot [my
laptop, 2.25GHz Pentium M] was sluggish with Photoshop CS2 at 512 and 1Gb
RAM, but when upgraded to 2Gb [motherboard max] it noticeably improved.
Windows 32-bit has the 3-4Gb OS max though [including graphics card memory],
and only 64-bit OS goes beyond the 4Gb max [motherboard dependent].

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov]
Sent: 14 April 2009 21:43
To: kjmorris-at-well.ox.ac.uk

Hi Kirsten,

You and your students will be fine with PowerPoint. For posters larger
than what PowerPoint's setup allows, we scale a little down, keeping
the desired proportion, then we increase the size (e.g., 123%) during
printing.

I have been using PP for posters since 2000, with different printers.
Until 2-3 years ago, we would have to go with our files to a
centralized printing facility, but now we have our own large HP
printer, and even new users have little problem using it from their
desktops. I've been meaning to switch to Adobe InDesign for posters
for years, but guess what, whenever the time comes to make a new
poster, there is always a hurry, and never enough time to learn new
software! I am neither lover or hater of Microsoft products, use them
as a tool, and PPoint has been very convenient for us.

I recommend making all adjustments to your photos while still in
Photoshop, and all labels, lettering, and scale in PowerPoint. This
way it'll be easier to do late adjustments like font size. We switch
PS color management off and try to achieve the desired gray levels on
all images. Once everything is to our liking, we reduce the pixel size
and save under different name.

Now, to your particular question - we aim for 150-200 pixels per inch
in the resulting full-size poster print. That's from some early
testing we did. We set picture size in PS, in inches, to what it
approximately is going to be in the final print and then set
resolution to 150-200 ppi. Then save the new image separately as
TIFF, that's the best and should not cause any file size issue these
days. Then we insert (or drag-n-drop) those saved images onto the
poster in PPoint. It is very common to have to resize the image a bit
this or that way before the poster is finished, that's why the ppi
range. 175 ppi is a good starting number. You'll get a feeling after a
few posters. Don't be afraid to explore lower ppi - as long as you see
all relevant features, of course, images printed from lower ppi tend
to look better than oversampled (too high ppi) images on a poster.
Below 150 ppi in the final print, you may notice the reduced
resolution, but it won't look bad. As long as you use TIFFs,
PowerPoint will do nice smoothening, and there'll be no pixelation.

Good luck.
Please don't hesitate to contact me directly if I wasn't clear,
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com}
} Date: April 13, 2009 6:14:52 PM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
} Reply-To: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com}
}
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Hi All,
}
} I'm getting ready to "teach" my undergraduate EM class how to create
} a poster presentation of their work and I need a little educating as
} well. Could you please send me your sage advice on creating a poster
} in PowerPoint (that's what we are required to use) regarding the
} resolution of the electron micrographs? As background, we are
} developing TEM film, scanning the negatives, and manipulating them
} with Adobe Photoshop.
}
} Thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} Frostburg State University
}
} kalennon-at-frostburg.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
} 8, 20 -- Received: from web84001.mail.mud.yahoo.com
} (web84001.mail.mud.yahoo.com [68.142.206.171])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} id n3DME1fS026918
} 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01
} -0500
} 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009
} 22:14:01 -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} d=yahoo.com; s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr
} +0Nro1sSEP2MofzkAUoG+LFo=; h=Message-ID:X-YMail-OSG:Received:X-
} Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QdDLdklkKjl/
} vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/
} 5pfJcQHaRTqzv0F9Du06x80gL9WfK9tfgY5blF/
}
Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99ILd8f0frdvPOc
7pH66pXM
} =
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-
} Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
} 8, 20 -- b=CRJXqaYio8A65tWLDZzLUty0rsYt37M
} +
}
1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2mHCuOFFiQl7wcdQylo85G5dOudaeps122CC
hWR4mvwBpvW0zUNQt
} +9ZB+ajEXgx7b+Mpe2yAzlxL11rki3h2FKA/pqZL+/La5Y=;
} 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
} 8, 20 -- X-YMail-OSG:
}
GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4
WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3Y
CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbx
ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQt
DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
} 8, 20 -- Received: from [96.239.150.112] by
} web84001.mail.mud.yahoo.com via HTTP; Mon, 13 Apr 2009 15:14:00 PDT
} 8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/
} 0.7.289.1
} 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
} 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 8, 20 -- Subject: Advice: Resolution of micrographs for posters?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
10, 23 -- From vladislav_speransky-at-nih.gov Tue Apr 14 15:38:28 2009
10, 23 -- Received: from nihrelayxway2.hub.nih.gov
(nihrelayxway2.hub.nih.gov [128.231.90.107])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3EKcR9X023807
10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 15:38:27
-0500
10, 23 -- X-IronPortListener: NIH_Relay
10, 23 -- X-SBRS: None
10, 23 -- X-IronPort-AV: E=Sophos;i="4.40,186,1238990400";
10, 23 -- d="scan'208";a="83849612"
10, 23 -- Received: from helix.nih.gov ([128.231.2.3])
10, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 14 Apr 2009 16:38:27
-0400
10, 23 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov
[128.231.217.185])
10, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id
n3EKcRYj014498
10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 16:38:27
-0400
10, 23 -- Message-Id: {FD8A426D-3157-4DE4-8CB3-74D5112051DD-at-nih.gov}
10, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
10, 23 -- To: Microscopy-at-microscopy.com
10, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
10, 23 -- Content-Transfer-Encoding: 7bit
10, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
10, 23 -- Subject: Fwd: [Microscopy] Advice: Resolution of micrographs for
posters?
10, 23 -- Date: Tue, 14 Apr 2009 16:38:27 -0400
10, 23 -- References:
{D13763A4740B07428566B25D7E768DA69F1C1A49-at-NIHMLBX02.nih.gov}
10, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================


==============================Original Headers==============================
22, 22 -- From kjmorris-at-well.ox.ac.uk Wed Apr 15 06:26:19 2009
22, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FBQG4e031166
22, 22 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 06:26:17 -0500
22, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
22, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
22, 22 -- id 1Lu3Fv-0007OP-ST
22, 22 -- for microscopy-at-microscopy.com; Wed, 15 Apr 2009 12:26:16 +0100
22, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
22, 22 -- To: {microscopy-at-microscopy.com}
22, 22 -- References: {200904142043.n3EKh0Ir001615-at-ns.microscopy.com}
22, 22 -- Subject: RE: [Microscopy] Fwd: Advice: Resolution of micrographs for posters?
22, 22 -- Date: Wed, 15 Apr 2009 12:26:15 +0100
22, 22 -- Message-ID: {331636B55C43479E95C18D815613E408-at-CytoWhizz}
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain;
22, 22 -- charset="us-ascii"
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Mailer: Microsoft Office Outlook 11
22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
22, 22 -- Thread-Index: Acm9QZqJk9tmskdvQoCnBp5RqqBW4gAeS60g
22, 22 -- In-Reply-To: {200904142043.n3EKh0Ir001615-at-ns.microscopy.com}
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Wed, 15 Apr 2009 13:46:15 -0500
Subject: [Microscopy] Re: Miller indices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lew,

What you are saying comes from the definition of reciprocal and direct
space and it is perfectly logical.
The problem is as you point out, that it is confusing and also it is
not always used in the literature in consistent fashion.

The confusing points arise usually for non-cubic and especially for
non-orthogonal crystal systems.

Consider a monoclinic crystal with beta the angle of monoclinicity. A
diffraction pattern which consists of 100 and 010 reflections is
defined as [001] zone axis, that is the el. beam is parallel to the
normal of the plane [001] in reciprocal space and that is what we call
the beam direction denoted usually B=[001].
The [001] direction in real space and the vector B, the normal to the
[001] plane in reciprocal space are parallel, but (001) plane in
direct space is not necessarily parallel to the [001] plane in
reciprocal space.

So when one writes [001] it is quite ambiguous whether we refer to
plane or direction until one specifies that it refers to either direct
or reciprocal space.
All this defies the purpose of notation. If we need to use narrative
clarification or the meaning of the context to make a notation clear
then that notation is useless. In trying to avoid such confusing point
in the literature are accepted different approaches e.g. the use of
asterisk to denote directions e.g. 001* would define a vector normal
to the (001) plane to avoid the need to write (001) and specify that
it is not the plane in real space that is referred to but actually the
normal to it.

So if we use some consistent notation for example denoting reflections
in reciprocal space as [001]* which will be read as "the normal to the
(001) planes in real space with magnitude 1/d001" and (001)* - the
plane in reciprocal space normal to the [001] direction in real space.
Then no context clarification or explicit explanation would be
necessary.

Krassimir.
================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel: 951 827 2998
fax: 951 827 2489

bozhilov-at-ucr.edu
==================================

On Apr 13, 2009, at 6:13 AM, Lew Rabenberg wrote:

} The notation is definitely not standard, but I simply invert real
} and reciprocal space.
}
} Real direction [uvw], {uvw}
} Real plane (hkl), {hkl}
}
} Reciprocal direction (hkl), {hkl}
} Reciprocal plane [uvw], {uvw}
}
} I do this because (i) planes in direct space are closely related to
} directions in reciprocal space, and (ii) it maintains the elegant
} symmetry/duality.
}
} It does cause some cognitive dissonance when the parameter being
} described (i.e. momentum) is clearly a vector, but defined in
} reciprocal space. Then, the natural tendency to denote a vector in
} "hard", square brackets is confronted by the factor that the vector
} is in the wrong space.
}
} I can deal with the cognitive dissonance.
}
} Lew Rabenberg
}
} On Apr 12, 2009, at 6:21 PM, bozhilov-at-ucr.edu wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} }
} } I hoping to get input about a certain consensus on the issue of
} } using
} } Miller indices for notation of crystallographic planes and
} } directions
} } in the TEM literature.
} }
} } The conventions about Miller indices notation in real space are
} } relatively clear. For example planes are denoted by round brackets
} } e.g. (100), directions by square brackets: [100], symmetrically
} } equivalent planes by curly brackets : {100}, and symmetrically
} } equivalent directions by angle brackets: {100} .
} }
} } The situation becomes confusing when reciprocal space notations are
} } introduced.
} } The most widely accepted convention as far as I am aware is that
} } planes in reciprocal space or reflections are denoted with Miller
} } indices without brackets e.g. 100.
} } Directions are denoted with asterisk as superscript e.g. 100* but
} } also [100]* is used, so which one should be used? If it is [100]*
} } then denoting reflections without brackets is inconsistent, it should
} } be (100)*.
} } Using curly brackets for denoting planes/reflections in reciprocal
} } space is not proper, but how should symmetrically equivalent planes/
} } reflections in reciprocal space be denoted? Maybe by using asterisk
} } but if brackets are not proper for specific planes/reflections in
} } reciprocal space then why they should be proper for curly brackets?
} } Similar question arises for the notation of symmetrically equivalent
} } directions in reciprocal space.
} }
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } tel. 951 827 2998
} } fax 951 827 2489
} } bozhilov-at-ucr.edu
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 22 -- From bozhilov-at-ucr.edu Sun Apr 12 18:19:46 2009
} } 9, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
} } [138.23.226.212])
} } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n3CNJjRf026159
} } 9, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2009
} } 18:19:46 -0500
} } 9, 22 -- Received: from [192.168.100.104]
} } (vpn-138-23-234-83.ucr.edu [138.23.234.83])
} } 9, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
} } 9, 22 -- with ESMTP id DPT77235 (AUTH bozhilov-at-ucr.edu)
} } 9, 22 -- for {Microscopy-at-microscopy.com} ;
} } 9, 22 -- Sun, 12 Apr 2009 16:19:45 -0700 (PDT)
} } 9, 22 -- Message-Id: {87820979-F803-463D-AF2E-F6F989C56C59-at-ucr.edu}
} } 9, 22 -- From: KN Bozhilov {bozhilov-at-ucr.edu}
} } 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} } 9, 22 -- In-Reply-To: {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
} } 9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} } delsp=yes
} } 9, 22 -- Content-Transfer-Encoding: 7bit
} } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} } 9, 22 -- Subject: Miller indices
} } 9, 22 -- X-Priority: 3
} } 9, 22 -- Date: Sun, 12 Apr 2009 16:19:44 -0700
} } 9, 22 -- References:
} } {200805082342.m48NgkeG022691-at-ns.microscopy.com}
} } {012701c8b269$5aa28650$b99fcb96-at-siap6segs20pa8} {457BCD4F-3FD9-4599-8363-2AF1A5775159-at-ucr.edu
} } } {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
} } 9, 22 -- X-Mailer: Apple Mail (2.930.3)
} } 9, 22 -- X-Junkmail-Status: score=0/50, host=
} } ==============================End of -
} } Headers==============================
}


==============================Original Headers==============================
12, 20 -- From bozhilov-at-ucr.edu Wed Apr 15 13:46:15 2009
12, 20 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FIkERx030538
12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 13:46:15 -0500
12, 20 -- Received: from [138.23.185.168] ([138.23.185.168])
12, 20 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
12, 20 -- with ESMTP id DQF40319 (AUTH bozhilov-at-ucr.edu);
12, 20 -- Wed, 15 Apr 2009 11:46:13 -0700 (PDT)
12, 20 -- Message-Id: {7EBDB4FE-CF5F-43F3-ADE0-2CABCE388D8E-at-ucr.edu}
12, 20 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
12, 20 -- To: Lew Rabenberg {lew.rabenberg-at-mail.utexas.edu} , microscopy-at-microscopy.com
12, 20 -- In-Reply-To: {2509DB54-7D1E-43BC-8A22-49B49CEF636F-at-mail.utexas.edu}
12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 20 -- Content-Transfer-Encoding: 7bit
12, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3)
12, 20 -- Subject: Re: [Microscopy] Miller indices
12, 20 -- Date: Wed, 15 Apr 2009 11:46:12 -0700
12, 20 -- References: {200904122321.n3CNLwqH029261-at-ns.microscopy.com} {2509DB54-7D1E-43BC-8A22-49B49CEF636F-at-mail.utexas.edu}
12, 20 -- X-Mailer: Apple Mail (2.930.3)
12, 20 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================




From: cni-at-udel.edu
Date: Wed, 15 Apr 2009 14:38:50 -0500
Subject: [Microscopy] Re: Miller indices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Enough confusion appears to be added in the discussion. I think simply the
following notations are usually agreed, especially in physical sciences:

1.
In real space:

(hkl) one plane
{hkl} family of equivalent planes
And also, it is usually necessary to differentiate (hkl) from (-h-k-l)

[uvw] one direction
{uvw} family of equivalent directions
[uvw] and [-u-v-w] are usually NOT differentiated from each other

2.
In reciprocal space:

Vector g* or any r* expressed as g*(r*) = ha* + kb* + lc*

In diffraction pattern:
The end point of a reciprocal vector (reciprocal lattice node), or a
diffraction spot, or a diffraction ring, is labeled as hkl NO BRACKETS
AT ALL

3.
Zone of a single crystal pattern is expressed as
[uvw]
which is (more or less) the electron beam direction in REAL SPACE. Square
brackets are necessary!

Also, context is important. Hope this clarifies.


Chaoying Ni, PhD
W.M. Keck Electron Microscopy Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept. of Materials Sci. and Eng.
University of Delaware
Newark, DE 19716
(302) 831-2318 (Phone) (302) 831-4545 (Fax)
http://eml.masc.udel.edu



-----Original Message-----
X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
Sent: Wednesday, April 15, 2009 2:49 PM
To: cni-at-UDel.Edu

Lew,

What you are saying comes from the definition of reciprocal and direct
space and it is perfectly logical.
The problem is as you point out, that it is confusing and also it is
not always used in the literature in consistent fashion.

The confusing points arise usually for non-cubic and especially for
non-orthogonal crystal systems.

Consider a monoclinic crystal with beta the angle of monoclinicity. A
diffraction pattern which consists of 100 and 010 reflections is
defined as [001] zone axis, that is the el. beam is parallel to the
normal of the plane [001] in reciprocal space and that is what we call
the beam direction denoted usually B=[001].
The [001] direction in real space and the vector B, the normal to the
[001] plane in reciprocal space are parallel, but (001) plane in
direct space is not necessarily parallel to the [001] plane in
reciprocal space.

So when one writes [001] it is quite ambiguous whether we refer to
plane or direction until one specifies that it refers to either direct
or reciprocal space.
All this defies the purpose of notation. If we need to use narrative
clarification or the meaning of the context to make a notation clear
then that notation is useless. In trying to avoid such confusing point
in the literature are accepted different approaches e.g. the use of
asterisk to denote directions e.g. 001* would define a vector normal
to the (001) plane to avoid the need to write (001) and specify that
it is not the plane in real space that is referred to but actually the
normal to it.

So if we use some consistent notation for example denoting reflections
in reciprocal space as [001]* which will be read as "the normal to the
(001) planes in real space with magnitude 1/d001" and (001)* - the
plane in reciprocal space normal to the [001] direction in real space.
Then no context clarification or explicit explanation would be
necessary.

Krassimir.
================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel: 951 827 2998
fax: 951 827 2489

bozhilov-at-ucr.edu
==================================

On Apr 13, 2009, at 6:13 AM, Lew Rabenberg wrote:

} The notation is definitely not standard, but I simply invert real
} and reciprocal space.
}
} Real direction [uvw], {uvw}
} Real plane (hkl), {hkl}
}
} Reciprocal direction (hkl), {hkl}
} Reciprocal plane [uvw], {uvw}
}
} I do this because (i) planes in direct space are closely related to
} directions in reciprocal space, and (ii) it maintains the elegant
} symmetry/duality.
}
} It does cause some cognitive dissonance when the parameter being
} described (i.e. momentum) is clearly a vector, but defined in
} reciprocal space. Then, the natural tendency to denote a vector in
} "hard", square brackets is confronted by the factor that the vector
} is in the wrong space.
}
} I can deal with the cognitive dissonance.
}
} Lew Rabenberg
}
} On Apr 12, 2009, at 6:21 PM, bozhilov-at-ucr.edu wrote:
}
} }
} }
} }
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} }
} } I hoping to get input about a certain consensus on the issue of
} } using
} } Miller indices for notation of crystallographic planes and
} } directions
} } in the TEM literature.
} }
} } The conventions about Miller indices notation in real space are
} } relatively clear. For example planes are denoted by round brackets
} } e.g. (100), directions by square brackets: [100], symmetrically
} } equivalent planes by curly brackets : {100}, and symmetrically
} } equivalent directions by angle brackets: {100} .
} }
} } The situation becomes confusing when reciprocal space notations are
} } introduced.
} } The most widely accepted convention as far as I am aware is that
} } planes in reciprocal space or reflections are denoted with Miller
} } indices without brackets e.g. 100.
} } Directions are denoted with asterisk as superscript e.g. 100* but
} } also [100]* is used, so which one should be used? If it is [100]*
} } then denoting reflections without brackets is inconsistent, it should
} } be (100)*.
} } Using curly brackets for denoting planes/reflections in reciprocal
} } space is not proper, but how should symmetrically equivalent planes/
} } reflections in reciprocal space be denoted? Maybe by using asterisk
} } but if brackets are not proper for specific planes/reflections in
} } reciprocal space then why they should be proper for curly brackets?
} } Similar question arises for the notation of symmetrically equivalent
} } directions in reciprocal space.
} }
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } tel. 951 827 2998
} } fax 951 827 2489
} } bozhilov-at-ucr.edu
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 22 -- From bozhilov-at-ucr.edu Sun Apr 12 18:19:46 2009
} } 9, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
} } [138.23.226.212])
} } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n3CNJjRf026159
} } 9, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2009
} } 18:19:46 -0500
} } 9, 22 -- Received: from [192.168.100.104]
} } (vpn-138-23-234-83.ucr.edu [138.23.234.83])
} } 9, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
} } 9, 22 -- with ESMTP id DPT77235 (AUTH bozhilov-at-ucr.edu)
} } 9, 22 -- for {Microscopy-at-microscopy.com} ;
} } 9, 22 -- Sun, 12 Apr 2009 16:19:45 -0700 (PDT)
} } 9, 22 -- Message-Id: {87820979-F803-463D-AF2E-F6F989C56C59-at-ucr.edu}
} } 9, 22 -- From: KN Bozhilov {bozhilov-at-ucr.edu}
} } 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} } 9, 22 -- In-Reply-To: {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
} } 9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} } delsp=yes
} } 9, 22 -- Content-Transfer-Encoding: 7bit
} } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} } 9, 22 -- Subject: Miller indices
} } 9, 22 -- X-Priority: 3
} } 9, 22 -- Date: Sun, 12 Apr 2009 16:19:44 -0700
} } 9, 22 -- References:
} } {200805082342.m48NgkeG022691-at-ns.microscopy.com}
} } {012701c8b269$5aa28650$b99fcb96-at-siap6segs20pa8}
{457BCD4F-3FD9-4599-8363-2AF1A5775159-at-ucr.edu
} } } {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8}
} } 9, 22 -- X-Mailer: Apple Mail (2.930.3)
} } 9, 22 -- X-Junkmail-Status: score=0/50, host=
} } ==============================End of -
} } Headers==============================
}


==============================Original Headers==============================
12, 20 -- From bozhilov-at-ucr.edu Wed Apr 15 13:46:15 2009
12, 20 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
[138.23.226.212])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3FIkERx030538
12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 13:46:15
-0500
12, 20 -- Received: from [138.23.185.168] ([138.23.185.168])
12, 20 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
12, 20 -- with ESMTP id DQF40319 (AUTH bozhilov-at-ucr.edu);
12, 20 -- Wed, 15 Apr 2009 11:46:13 -0700 (PDT)
12, 20 -- Message-Id: {7EBDB4FE-CF5F-43F3-ADE0-2CABCE388D8E-at-ucr.edu}
12, 20 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
12, 20 -- To: Lew Rabenberg {lew.rabenberg-at-mail.utexas.edu} ,
microscopy-at-microscopy.com
12, 20 -- In-Reply-To:
{2509DB54-7D1E-43BC-8A22-49B49CEF636F-at-mail.utexas.edu}
12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
12, 20 -- Content-Transfer-Encoding: 7bit
12, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3)
12, 20 -- Subject: Re: [Microscopy] Miller indices
12, 20 -- Date: Wed, 15 Apr 2009 11:46:12 -0700
12, 20 -- References: {200904122321.n3CNLwqH029261-at-ns.microscopy.com}
{2509DB54-7D1E-43BC-8A22-49B49CEF636F-at-mail.utexas.edu}
12, 20 -- X-Mailer: Apple Mail (2.930.3)
12, 20 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================



==============================Original Headers==============================
32, 29 -- From cni-at-udel.edu Wed Apr 15 14:38:50 2009
32, 29 -- Received: from md1.nss.udel.edu (md1.nss.udel.edu [128.175.1.11])
32, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FJcoW0015827
32, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 14:38:50 -0500
32, 29 -- Received: from Suba (em076-119.engg.udel.edu [128.175.119.76])
32, 29 -- by md1.nss.udel.edu (MOS 3.10.2-GA)
32, 29 -- with ESMTP id FWB33378;
32, 29 -- Wed, 15 Apr 2009 15:38:47 -0400 (EDT)
32, 29 -- From: "Chaoying Ni" {cni-at-udel.edu}
32, 29 -- To: {Microscopy-at-microscopy.com}
32, 29 -- Cc: {bozhilov-at-ucr.edu}
32, 29 -- Subject: RE: [Microscopy] Re: Miller indices
32, 29 -- Date: Wed, 15 Apr 2009 15:38:48 -0400
32, 29 -- Organization: University of Delaware
32, 29 -- Message-ID: {34FE9F756E52421FB2483AFD7CB5593E-at-Suba}
32, 29 -- MIME-Version: 1.0
32, 29 -- Content-Type: text/plain;
32, 29 -- charset="us-ascii"
32, 29 -- X-Priority: 3 (Normal)
32, 29 -- X-MSMail-Priority: Normal
32, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
32, 29 -- Thread-Index: Acm9+tDXlj2IR7sGSrayT4iQEDYx4AAAgnmA
32, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
32, 29 -- Importance: Normal
32, 29 -- In-Reply-To: {200904151848.n3FImnkI032761-at-ns.microscopy.com}
32, 29 -- X-Antivirus: avast! (VPS 090415-0, 04/15/2009), Outbound message
32, 29 -- X-Antivirus-Status: Clean
32, 29 -- Content-Transfer-Encoding: 8bit
32, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3FJcoW0015827
==============================End of - Headers==============================




From: sergey-at-seas.ucla.edu
Date: Wed, 15 Apr 2009 18:15:43 -0500
Subject: [Microscopy] viaWWW: JEOL TEM holders for trade

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sergey-at-seas.ucla.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sergey-at-seas.ucla.edu
Name: Sergey Prikhodko

Organization: UCLA

Title-Subject: [Filtered] TEM holders

Question: Dear all,

I have used JEOL TEM holders (in operational condition) for trade:
1)single-tilt EM-SQH10;
2)double-tilt EM-STH10;
3)rotational EM-SRH10.


Login Host: 128.97.83.27
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Apr 15 18:15:43 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FNFgob021494
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 18:15:43 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c60c1b024202-at-[206.69.208.22]}
8, 11 -- Date: Wed, 15 Apr 2009 18:15:41 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: sergey-at-seas.ucla.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: JEOL TEM holders for trade
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: siverson-at-minntech.com
Date: Wed, 15 Apr 2009 18:16:21 -0500
Subject: [Microscopy] viaWWW: Hitachi S-800 SEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both siverson-at-minntech.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: siverson-at-minntech.com
Name: Sandra Iverson

Organization: Minntech Corporation

Title-Subject: [Filtered] Hitachi S-800 SEM available

Question: We stopped using our Hitachi S-800 SEM two years ago when
we purchased a tabletop model and, coincidentally, the video went out
in our S-800. The unit has been sitting idle since then and it's time
for it to go.

This microscope is available to any organization willing to come in,
disassemble it, and arrange for shipping. Preference will be given to
universities.



Login Host: 155.229.55.9
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Apr 15 18:16:21 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FNGIBr022149
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 18:16:21 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c60c1b314d1e-at-[206.69.208.22]}
9, 11 -- Date: Wed, 15 Apr 2009 18:16:17 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: siverson-at-minntech.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Hitachi S-800 SEM available
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 16 Apr 2009 09:30:21 -0500
Subject: [Microscopy] RE: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our school is using PowerPoint as default software for creating posters for many years.

While 300 ppi is a "golden standard" for printers (glossy magazines), it is surely overkill for posters printed with inkjet printers. From my experience printing images from XL30 SEM (about 1400*1000 pixels) at 11" image size (i.e. 127 ppi) produce good results. Even 100 ppi on poster could be good enough. But for line drawings you need higher resolution.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: dkloos-at-parallaxray.com [mailto:dkloos-at-parallaxray.com]
} Sent: Tuesday, April 14, 2009 12:57 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I just took my 'slides' to Kinko's and had them printed up to
} desired size.
} Worked great.
}
} Don Kloos
} VP Sales, Marketing, Business Development Parallax Research, Inc.
}
}
}
} Sales & Marketing
} 16478 Beach Blvd. #330
} Westminster, California, 92683-7860 USA
}
} TOLL FREE 1 866 581-XRAY (9729)
} Telephone 1 714 897-9779
} Fax 1 714 897-1421
} Email: dkloos-at-parallaxray.com
} SKYPE: don.kloos
} Website: http://www.parallaxray.com
}
}
}
} -----Original Message-----
} X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
} Sent: Tuesday, April 14, 2009 9:11 AM
} To: dkloos-at-parallaxray.com
} Subject: [Microscopy] RE: Advice: Resolution of micrographs
} for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} HI Kristen
} There is lots to recommend Power point but posters isn't its strongest
} suite.
} Our media specialist tells me the Power Point's output size
} is one of its
} weak points
} Poster size width is determined by the printer being used
} (our is 43 inches)
} while the maximum length is 56 inches
} Lengths greater than 56 leave the printer scratching its head
} though there
} are work arounds.
}
} Rick,
}
} Richard Harris, Manager - Imaging and Data Systems
} The Biotron - Experimental Climate Change Research
} University of Western Ontario,
} London Ontario, CANADA.
} N6A 5B7
} Ph.  519-661-2111 ext. 86780
} Fax  519-661-3935
} e-mail rjharris-at-uwo.ca
} web: www.biotron.uwo.ca
}
}
} -----Original Message-----
} X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com]
} Sent: Monday, April 13, 2009 6:19 PM
} To: rjharris-at-uwo.ca
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
}
} Hi All,
}
} I'm getting ready to "teach" my undergraduate EM class how to create a
} poster presentation of their work and I need a little
} educating as well.
} Could you please send me your sage advice on creating a
} poster in PowerPoint
} (that's what we are required to use) regarding the resolution of the
} electron micrographs? As background, we are developing TEM
} film, scanning
} the negatives, and manipulating them with Adobe Photoshop.
}
} Thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} Frostburg State University
}
} kalennon-at-frostburg.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
} 8, 20 -- Received: from web84001.mail.mud.yahoo.com
} (web84001.mail.mud.yahoo.com [68.142.206.171])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id
} n3DME1fS026918
} 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr
} 2009 17:14:01
} -0500
} 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr
} 2009 22:14:01
} -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256;
} c=relaxed/relaxed; d=yahoo.com;
} s=s1024; t=1239660840;
} bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=;
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
o:MIME-Version
} :Content-Type;
} b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqz
} v0F9Du06x80gL9
} WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3W
} Ril6P7zxv9Mv99
} ILd8f0frdvPOc7pH66pXM=
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 --
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
o:MIME-Version
} :Content-Type;
} 8, 20 --
} b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf
57dVuhsdE69j2m
} HCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx
7b+Mpe2yAzlxL1
} 1rki3h2FKA/pqZL+/La5Y=;
} 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
} 8, 20 -- X-YMail-OSG:
} GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48Ldabp
} A7uxHRhCirAh.4
} WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtng
} fec2j2sPU.vV3Y
} CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw
} 6Kks1l4MQ2VXbx
} ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LL
zFcha2HwyW_LQt
} DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
} 8, 20 -- Received: from [96.239.150.112] by
} web84001.mail.mud.yahoo.com via
} HTTP; Mon, 13 Apr 2009 15:14:00 PDT
} 8, 20 -- X-Mailer: YahooMailClassic/5.2.15
} YahooMailWebService/0.7.289.1
} 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
} 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 8, 20 -- Subject: Advice: Resolution of micrographs for posters?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 19, 29 -- From rjharris-at-uwo.ca Tue Apr 14 10:58:49 2009
} 19, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca
} [129.100.74.146])
} 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id
} n3EFwmH3008207
} 19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr
} 2009 10:58:49
} -0500
} 19, 29 -- MIME-version: 1.0
} 19, 29 -- Content-type: text/plain; charset=iso-8859-1
} 19, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri
} [172.29.32.39])
} 19, 29 -- by harpo.mail.uwo.pri
} 19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04
} (built Sep 26
} 2008; 32bit))
} 19, 29 -- with ESMTP id {0KI300L1FLPZ0Y00-at-harpo.mail.uwo.pri} for
} 19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009
} 11:58:47 -0400 (EDT)
} 19, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca
} [129.100.52.17])
} 19, 29 -- by harpo.mail.uwo.pri
} 19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04
} (built Sep 26
} 2008; 32bit))
} 19, 29 -- with ESMTPSA id {0KI300K3ZLPZEI00-at-harpo.mail.uwo.pri} for
} 19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009
} 11:58:47 -0400 (EDT)
} 19, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
} 19, 29 -- To: kamlennon-at-yahoo.com
} 19, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
} 19, 29 -- References: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
} 19, 29 -- In-reply-to: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
} 19, 29 -- Subject: RE: [Microscopy] Advice: Resolution of
} micrographs for
} posters?
} 19, 29 -- Date: Tue, 14 Apr 2009 11:58:47 -0400
} 19, 29 -- Message-id: {00f601c9bd19$e5888010$b0998030$-at-ca}
} 19, 29 -- X-Mailer: Microsoft Office Outlook 12.0
} 19, 29 -- Thread-index: Acm8hexsPjIfYHJqSt6Tn+qeWbWeggAk10Nw
} 19, 29 -- Content-language: en-us
} 19, 29 -- Content-Transfer-Encoding: 8bit
} 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n3EFwmH3008207
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 29, 31 -- From dkloos-at-parallaxray.com Tue Apr 14 12:56:32 2009
} 29, 31 -- Received: from cp18.heritagewebdesign.com
} (cp18.heritagewebdesign.com [209.90.77.54])
} 29, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n3EHuVqD014372
} 29, 31 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr
} 2009 12:56:31 -0500
} 29, 31 -- Received: from user-0c8gg59.cable.mindspring.com
} ([24.136.64.169] helo=donl)
} 29, 31 -- by cp18.heritagewebdesign.com with esmtpa (Exim
} 4.69 (FreeBSD))
} 29, 31 -- (envelope-from {dkloos-at-parallaxray.com} )
} 29, 31 -- id 1Ltms8-00034B-Nl; Tue, 14 Apr 2009 11:56:36 -0600
} 29, 31 -- Reply-To: {dkloos-at-parallaxray.com}
} 29, 31 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
} 29, 31 -- To: {microscopy-at-microscopy.com}
} 29, 31 -- Cc: {rjharris-at-uwo.ca}
} 29, 31 -- References: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
} 29, 31 -- Subject: RE: [Microscopy] RE: Advice: Resolution of
} micrographs for posters?
} 29, 31 -- Date: Tue, 14 Apr 2009 10:56:24 -0700
} 29, 31 -- Organization: Parallax Research
} 29, 31 -- Message-ID: {2B5B9B340BD2412FB23D2C53AF29F19E-at-donl}
} 29, 31 -- MIME-Version: 1.0
} 29, 31 -- Content-Type: text/plain;
} 29, 31 -- charset="iso-8859-1"
} 29, 31 -- X-Mailer: Microsoft Office Outlook 11
} 29, 31 -- In-Reply-To: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
} 29, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 29, 31 -- Thread-Index: Acm9G64h6c8cFDLwQ/+utZ/f7mH7TwADom1A
} 29, 31 -- X-AntiAbuse: This header was added to track abuse,
} please include it with any abuse report
} 29, 31 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
} 29, 31 -- X-AntiAbuse: Original Domain - microscopy.com
} 29, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
} 29, 31 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
} 29, 31 -- Content-Transfer-Encoding: 8bit
} 29, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit
} by ns.microscopy.com id n3EHuVqD014372
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Thu Apr 16 09:30:21 2009
8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GEUKSv013479
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 09:30:21 -0500
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Thu, 16 Apr 2009 09:30:20 -0500
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="iso-8859-1"
8, 25 -- Subject: RE: [Microscopy] Advice: Resolution of micrographs for posters?
8, 25 -- Date: Thu, 16 Apr 2009 09:30:20 -0500
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB825-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200904141757.n3EHv4oA015903-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] Advice: Resolution of micrographs for posters?
8, 25 -- Thread-Index: Acm9Kmz+bZXCpkekRKOXFcmy6Ymb4QBcowPg
8, 25 -- References: {200904141757.n3EHv4oA015903-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 16 Apr 2009 14:30:20.0490 (UTC) FILETIME=[DEDD6AA0:01C9BE9F]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3GEUKSv013479
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 16 Apr 2009 11:21:11 -0500
Subject: [Microscopy] insurance - based contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody,

It looks like we a pushed (gently, for now) to consider possibility of
switching our service contracts to insurance - based contracts. So, now
I am very interested in opinions on this type of servicing microscopes -
SEM and TEM.

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
8, 23 -- From DusevichV-at-umkc.edu Thu Apr 16 11:21:11 2009
8, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GGLA3D031629
8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 11:21:10 -0500
8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Thu, 16 Apr 2009 11:21:03 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: insurance - based contracts
8, 23 -- Date: Thu, 16 Apr 2009 11:21:03 -0500
8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB826-at-KC-MSX1.kc.umkc.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: insurance - based contracts
8, 23 -- Thread-Index: Acm+r1ZWS/e5rwhqQOiH1pCF8nC7Vw==
8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 16 Apr 2009 16:21:03.0925 (UTC) FILETIME=[56A92650:01C9BEAF]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3GGLA3D031629
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 16 Apr 2009 12:13:24 -0500
Subject: [Microscopy] insurance - based contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Vladimir,

Push back----and don't be gentle. We tried this a number of years ago
and it was a disaster. It once took us nine months to schedule
preventive maintenance, since an insurance provider wasn't paying its
bills and the service provider understandably went on strike.

Despite what they told us when they were selling us these contracts, the
procedure to get service was MUCH more complicated and time-consuming
and we were given a much lower priority by the vendors than their
service contract holders were. You may have to justify service and
parts before they will authorize it.

Maybe we just had a poor experience and things are better now, but the
day we went back on vendor service contracts for our scopes was one of
the happiest days of my life.

Have you considered third-party service providers contracts? Some
people say they are very happy with these. I have no personal
experience with them, however.

Good luck,
Randy



-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: Thursday, April 16, 2009 11:23 AM
To: Tindall, Randy D.

Hi everybody,

It looks like we a pushed (gently, for now) to consider possibility of
switching our service contracts to insurance - based contracts. So, now
I am very interested in opinions on this type of servicing microscopes -
SEM and TEM.

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original
Headers==============================
8, 23 -- From DusevichV-at-umkc.edu Thu Apr 16 11:21:11 2009
8, 23 -- Received: from kc-msxproto3.kc.umkc.edu
(kc-msxproto3.kc.umkc.edu [134.193.44.10])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n3GGLA3D031629
8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009
11:21:10 -0500
8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Thu, 16 Apr 2009 11:21:03 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: insurance - based contracts
8, 23 -- Date: Thu, 16 Apr 2009 11:21:03 -0500
8, 23 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB826-at-KC-MSX1.kc.umkc.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: insurance - based contracts
8, 23 -- Thread-Index: Acm+r1ZWS/e5rwhqQOiH1pCF8nC7Vw==
8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 16 Apr 2009 16:21:03.0925 (UTC)
FILETIME=[56A92650:01C9BEAF]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3GGLA3D031629
==============================End of -
Headers==============================



==============================Original Headers==============================
23, 30 -- From TindallR-at-missouri.edu Thu Apr 16 12:13:24 2009
23, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GHDNFh014797
23, 30 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 12:13:23 -0500
23, 30 -- X-IronPort-Anti-Spam-Filtered: true
23, 30 -- X-IronPort-Anti-Spam-Result: ApoEAMkD50nRauUo/2dsb2JhbADBdAEJhyCITQGCOBGBMwaHXw
23, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
23, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 16 Apr 2009 12:13:23 -0500
23, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
23, 30 -- Thu, 16 Apr 2009 12:13:23 -0500
23, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
23, 30 -- Content-class: urn:content-classes:message
23, 30 -- MIME-Version: 1.0
23, 30 -- Content-Type: text/plain;
23, 30 -- charset="us-ascii"
23, 30 -- Subject: RE: [Microscopy] insurance - based contracts
23, 30 -- Date: Thu, 16 Apr 2009 12:13:23 -0500
23, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8056-at-UM-XMAIL08.um.umsystem.edu}
23, 30 -- In-Reply-To: {200904161622.n3GGMUou000786-at-ns.microscopy.com}
23, 30 -- X-MS-Has-Attach:
23, 30 -- X-MS-TNEF-Correlator:
23, 30 -- Thread-Topic: [Microscopy] insurance - based contracts
23, 30 -- Thread-Index: Acm+r5VZeArF7ApBSMmzvqCchezKgQABgVjQ
23, 30 -- References: {200904161622.n3GGMUou000786-at-ns.microscopy.com}
23, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 30 -- To: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
23, 30 -- Cc: {microscopy-at-microscopy.com}
23, 30 -- X-OriginalArrivalTime: 16 Apr 2009 17:13:23.0080 (UTC) FILETIME=[A5BE3080:01C9BEB6]
23, 30 -- Content-Transfer-Encoding: 8bit
23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3GHDNFh014797
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Thu, 16 Apr 2009 12:31:56 -0500
Subject: [Microscopy] Re: insurance - based contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vlaidmir.

I used one in a previous incarnation (job elsewhere). It was fine,
saved money, got the microscope company service.
Then we had a big claim and a diagnostic procedure -- looking for
stray magnetic fields. Suddenly the contract was worthless. The
insurance company refused to pay the claim (contrary to their
saleman's statements) because of the magnetic field survey. When I
explained why the survey and what it was, they (supposedly) referred
the claim to their engineers, who may be great on elevators and
escalators, but know nothing of EMs, who denied the claim.
They had paid the microscope company, but they were trying to get me
to re-imburse them.
I referred them to the University lawyers (said University having
several contracts with this company for various equipment).
I don't know how it finally ended, but I didn't give the insurance
company any money, and as soon as the contract was up, went to the
microscope company for a contract.

Where I am now, we have company contracts and no hint of insurance
contracts, and I'd fight them pump and electron to avoid changing.

Phil

} Hi everybody,
}
} It looks like we a pushed (gently, for now) to consider possibility of
} switching our service contracts to insurance - based contracts. So, now
} I am very interested in opinions on this type of servicing microscopes -
} SEM and TEM.
}
} Thank you,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
5, 26 -- From oshel1pe-at-cmich.edu Thu Apr 16 12:31:56 2009
5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GHVu5f029068
5, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 12:31:56 -0500
5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3GHVsnD000652;
5, 26 -- Thu, 16 Apr 2009 13:31:54 -0400
5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 26 -- Thu, 16 Apr 2009 13:31:50 -0400
5, 26 -- Mime-Version: 1.0
5, 26 -- Message-Id: {f06240802c60d1a08643c-at-[141.209.160.249]}
5, 26 -- In-Reply-To: {200904161625.n3GGPga9005060-at-ns.microscopy.com}
5, 26 -- References: {200904161625.n3GGPga9005060-at-ns.microscopy.com}
5, 26 -- Date: Thu, 16 Apr 2009 13:31:48 -0400
5, 26 -- To: DusevichV-at-umkc.edu
5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 26 -- Subject: Re: [Microscopy] insurance - based contracts
5, 26 -- Cc: Microscopy-at-microscopy.com
5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 26 -- X-OriginalArrivalTime: 16 Apr 2009 17:31:50.0748 (UTC) FILETIME=[39F6E9C0:01C9BEB9]
5, 26 -- X-Canit-CHI2: 0.00
5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 26 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
5, 26 -- X-CanItPRO-Stream: default
5, 26 -- X-Canit-Stats-ID: 11939391 - da1cb7a8bc4d
5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Thu, 16 Apr 2009 13:02:36 -0500
Subject: [Microscopy] Re: insurance - based contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vladimir-
I've always had contracts, but my colleague at a nearby institution
was forced to go with insurance. They have an older TEM and at one
point needed a major repair that took over 2 weeks....charged
hourly. The end result was a bill close to if not more than the
vendor's contract would have been. They also have trouble getting
their PM visits scheduled, since labs under contract do get priority.
Lee
}
} } Hi everybody,
} }
} } It looks like we a pushed (gently, for now) to consider possibility of
} } switching our service contracts to insurance - based contracts. So, now
} } I am very interested in opinions on this type of servicing microscopes -
} } SEM and TEM.
} }
} } Thank you,
} }
} } Vladimir
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 371 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } Phone: (816) 235-2072
} } Fax: (816) 235-5524
} } Web: http://www.umkc.edu/dentistry/microscopy
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576
}
} ==============================Original Headers==============================
} 5, 26 -- From oshel1pe-at-cmich.edu Thu Apr 16 12:31:56 2009
} 5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
} 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n3GHVu5f029068
} 5, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009
} 12:31:56 -0500
} 5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
} 5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP
} id n3GHVsnD000652;
} 5, 26 -- Thu, 16 Apr 2009 13:31:54 -0400
} 5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by
} egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
} 5, 26 -- Thu, 16 Apr 2009 13:31:50 -0400
} 5, 26 -- Mime-Version: 1.0
} 5, 26 -- Message-Id: {f06240802c60d1a08643c-at-[141.209.160.249]}
} 5, 26 -- In-Reply-To: {200904161625.n3GGPga9005060-at-ns.microscopy.com}
} 5, 26 -- References: {200904161625.n3GGPga9005060-at-ns.microscopy.com}
} 5, 26 -- Date: Thu, 16 Apr 2009 13:31:48 -0400
} 5, 26 -- To: DusevichV-at-umkc.edu
} 5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
} 5, 26 -- Subject: Re: [Microscopy] insurance - based contracts
} 5, 26 -- Cc: Microscopy-at-microscopy.com
} 5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} 5, 26 -- X-OriginalArrivalTime: 16 Apr 2009 17:31:50.0748 (UTC)
} FILETIME=[39F6E9C0:01C9BEB9]
} 5, 26 -- X-Canit-CHI2: 0.00
} 5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
} 5, 26 -- X-Spam-Score: -4.20 () [Hold at 5.00]
} L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
} 5, 26 -- X-CanItPRO-Stream: default
} 5, 26 -- X-Canit-Stats-ID: 11939391 - da1cb7a8bc4d
} 5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
} ==============================End of - Headers==============================


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
4, 33 -- From lcgould-at-med.cornell.edu Thu Apr 16 13:02:35 2009
4, 33 -- Received: from mail-gw1.med.cornell.edu (mail-gw1.med.cornell.edu [140.251.3.44])
4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GI2YGh011495
4, 33 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 13:02:35 -0500
4, 33 -- MIME-version: 1.0
4, 33 -- Content-transfer-encoding: 7BIT
4, 33 -- Content-type: text/plain; charset=us-ascii; format=flowed
4, 33 -- Received: from mpx5.med.cornell.edu ([140.251.11.120])
4, 33 -- by mail-gw1.med.cornell.edu
4, 33 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
4, 33 -- with ESMTP id {0KI70031KGS70G40-at-mail-gw1.med.cornell.edu} for
4, 33 -- microscopy-at-microscopy.com; Thu, 16 Apr 2009 14:02:34 -0400 (EDT)
4, 33 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
4, 33 -- by mpx5.med.cornell.edu
4, 33 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
4, 33 -- with ESMTPA id {0KI700HEZGS8AM50-at-mpx5.med.cornell.edu} for
4, 33 -- microscopy-at-microscopy.com; Thu, 16 Apr 2009 14:02:33 -0400 (EDT)
4, 33 -- X-PMX-Version: 5.5.2.365749, Antispam-Engine: 2.6.1.350677,
4, 33 -- Antispam-Data: 2009.4.16.174920
4, 33 -- X-Perlmx-Spam: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0,
4, 33 -- BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DOMAIN_OBFU_DOT 0,
4, 33 -- __C230066_P5 0, __CP_NOT_1 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0,
4, 33 -- __FRAUD_419_CONTACT_NUM 0, __HAS_MSGID 0, __KNOWN_PHONE_RU_812 0,
4, 33 -- __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
4, 33 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0'
4, 33 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
4, 33 -- Message-id: {p0623090cc60d21b3b6e3-at-[140.251.48.23]}
4, 33 -- In-reply-to: {200904161740.n3GHeIN8009039-at-ns.microscopy.com}
4, 33 -- References: {200904161740.n3GHeIN8009039-at-ns.microscopy.com}
4, 33 -- Date: Thu, 16 Apr 2009 14:02:28 -0400
4, 33 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
4, 33 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
4, 33 -- Subject: [Microscopy] Re: insurance - based contracts
==============================End of - Headers==============================




From: donald.gibbon-at-matcoinc.com
Date: Thu, 16 Apr 2009 18:01:26 -0500
Subject: [Microscopy] viaWWW: TEM powder electrron diffraction analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both donald.gibbon-at-matcoinc.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: donald.gibbon-at-matcoinc.com
Name: Donald L. Gibbon

Organization: MATCO Services Inc

Title-Subject: [Filtered] TEM powder electrron diffraction analysis

Question: In the olden days before SEM/EDS made it appear that just
anyone could identify almost anything in the materials world,
microscopists would use the diffraction capabilities of transmission
electron microscopes to distinguish amorphous and crystalline
substances and determine the d-spacings of the crystalline parts.
Powder patterns were routinely created using vapor-cast gold,
platinum or aluminum as internal standards and then analyzed by
measuring the diameters of the rings, converting the linear
measurements into d-spacings using a handy slide rule. I am sure many
of you do not believe this era is still within living memory. But I
assure you it is.

Today, only having access to SEM/EDS at my work place, I am
constantly frustrated by not being able to do what I used to do
routinely 40 years ago. For example, I want to determine the
crystalline species forming a thin layer on a copper coupon. Again,
in the olden days I would have used my evaporator to carbon-coat a
glass slide, hold it briefly in the fumes of HF, float the carbon off
on water, pick up a piece of the thin carbon film on a 3mm grid,
crudely scrape the coupon over the grid with a spatula to deposit a
powder on the carbon film, coat it with a thin layer of Al metal, put
it in the TEM and in about five minutes have a powder pattern.
Develop and print the pattern, measure it and have the list of
d-spacings in just a few more minutes. Many common species could be
identified from the patterns by inspection.

Today I could use Auger or EDS to identify the elemental components
of the film but I can't nail the structure/compound. If there's
enough material I might be able to determine the index of refraction
with oils (does any one else remember how to do that?)... but powder
electron diffraction is SO easy and so definitive!

To REALLY blow your minds, consider how few people have capabilities
to do reflection electron diffraction right off the surface of the
copper coupon, with the deposit actually in place. THAT is the
simplest technique in the world: just orient the sample surface
nearly vertical(parallel to the beam) and advance it into the beam.
You'll be amazed to see a diffraction pattern appear on the screen of
the microscope, just like Davison and Germer did when they determined
the wave nature of the electron in the 1920s. What a buzz!

With all that build up, is there anyone out there offering this TEM
diffraction service? I'd particularly like to know someone near
Pittsburgh, so I could be with them when they do the analysis. If you
have the equipment and don't know the technique, I'll show you how to
do it!


Login Host: 72.165.239.20
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Apr 16 18:01:26 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3GN1Om5007309
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 18:01:26 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c60d69339731-at-[206.69.208.22]}
11, 11 -- Date: Thu, 16 Apr 2009 18:01:22 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: donald.gibbon-at-matcoinc.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: TEM powder electrron diffraction analysis
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: joe.kintz-at-stress.com
Date: Fri, 17 Apr 2009 07:39:38 -0500
Subject: [Microscopy] Need very large chamber environmental SEM with EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vladimir,

Don't do this, you will be sorry.

I serviced TEM/SEM tools first working for Philips, then as independent,
servicing and moving all brands plus
associated instruments and equipment. You may get lucky for awhile and save
some money, until major problem happens. Such as defective HT tank for
example. Insurance will try to locate a used one, or an independent service
provider that will repair it. I did that. But insurance is unlikely to pay
tens of thousands of $$ when all else fails.

There is no miracle. This kind of insurance does not have enough base to be
a reliable one, even if they try hard- this will be mathematically
impossible in the end. It is difficult enough for an OEM to make service and
support business profitable while dealing directly with end users. How can
you add a middleman in-between and expect profit? Please don't do this.

If life comes to the point when your employer can't afford an OEM or a 3rd
party contract - do you own PMs and pay directly to service providers when
their help needed. And keep your fingers crossed that nothing major will go.
It sounds scary, but works better than insurance based service. Common sense
versus peace of mind.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
----- Original Message -----
X-from: {DusevichV-at-umkc.edu}
To: {vitalylazar-at-att.net}
Sent: Thursday, April 16, 2009 12:23 PM

I'm looking for a very large chambered environmental SEM with EDS
capabilities to examine a friable firebrick that is roughly 6 inches cubed.
Thank you for any leads.

Joe


==============================Original Headers==============================
3, 20 -- From Joe.Kintz-at-stress.com Fri Apr 17 07:39:38 2009
3, 20 -- Received: from mxgate.stress.com (mxgate.stress.com [12.96.4.229])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3HCdao0027652
3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 07:39:37 -0500
3, 20 -- Received: from jkintz ([172.16.12.133]) by mail.stress.com with Microsoft SMTPSVC(6.0.3790.1830); Fri, 17 Apr 2009 07:39:35 -0500
3, 20 -- From: "Joe Kintz" {joe.kintz-at-stress.com}
3, 20 -- To: {microscopy-at-microscopy.com}
3, 20 -- References: {200904170810.n3H8AeUW017439-at-ns.microscopy.com}
3, 20 -- Subject: Need very large chamber environmental SEM with EDS
3, 20 -- Date: Fri, 17 Apr 2009 07:39:35 -0500
3, 20 -- Message-ID: {00d601c9bf59$904e3990$850c10ac-at-stress.com}
3, 20 -- MIME-Version: 1.0
3, 20 -- Content-Type: text/plain;charset="us-ascii"
3, 20 -- Content-Transfer-Encoding: 7bit
3, 20 -- X-Mailer: Microsoft Office Outlook 11
3, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
3, 20 -- In-Reply-To: {200904170810.n3H8AeUW017439-at-ns.microscopy.com}
3, 20 -- Thread-Index: Acm/NARp9bon5zBDSzKJhqJf8azqcQAJSwhw
3, 20 -- X-OriginalArrivalTime: 17 Apr 2009 12:39:35.0465 (UTC) FILETIME=[9088D190:01C9BF59]
3, 20 -- X-SCA-Stop: [cust-96647FDDB5F74CA0B2173CACB4D94086,18,39,0]
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Fri, 17 Apr 2009 10:45:36 -0500
Subject: [Microscopy] Re: Need very large chamber environmental SEM with EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe,

Contact Jaret Frafjord ( frafjordjj-at-y12.doe.gov). He's got an sem you can
stand in.

Jeff Stewart
Materials Characterization Lab Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329


----- Original Message -----
X-from: {joe.kintz-at-stress.com}
To: {jeff-at-metallography.com}
Sent: Friday, April 17, 2009 8:40 AM

Most FEI systems I believe could handle a 6" cubed sample.  Especially
the 600 series sized system.  The FEI systems can also have EDS added.

Here's a sampling from their website.

http://www.fei.com/uploadedFiles/Documents/Content/2007_08_NNS_630_ds.pdf

http://www.fei.com/products/types/scanning-electron-microscope.aspx


Hope that helps.

} On Fri, Apr 17, 2009 at 6:53 AM, {joe.kintz-at-stress.com} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I'm looking for a very large chambered environmental SEM with EDS
} } capabilities to examine a friable firebrick that is roughly 6 inches cubed.
} } Thank you for any leads.
} }
} } Joe
} }
} }


==============================Original Headers==============================
8, 36 -- From ian.drucker-at-gmail.com Fri Apr 17 10:45:35 2009
8, 36 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.246])
8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3HFjZFq030514
8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 10:45:35 -0500
8, 36 -- Received: by rv-out-0708.google.com with SMTP id l33so936557rvb.30
8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 08:45:35 -0700 (PDT)
8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 36 -- d=gmail.com; s=gamma;
8, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references
8, 36 -- :from:date:message-id:subject:to:content-type
8, 36 -- :content-transfer-encoding;
8, 36 -- bh=w4/XykWgXigqxx0C7yzK2sQ6VZPpGirSVhI9PpCtCbA=;
8, 36 -- b=BIM1lsBKAyg5iy9T8gxXgP/fe6h9YfCZmaKDcvGhJJUsAIe7cSfG6N1YttlRQoftpH
8, 36 -- W7fiSnFmM1GCHr1C+sxHWmwKZ3dDS03uYQm+EmdzOukritZ6pXUZcA+TrN9OUI7FPpvO
8, 36 -- IYYwg1u3/UzGelKePxE785U3ialitqK7TSetU=
8, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 36 -- d=gmail.com; s=gamma;
8, 36 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to
8, 36 -- :content-type:content-transfer-encoding;
8, 36 -- b=w6rmnkP47rYv40Y8bk6RZg18DalsYbytTVeU6pSbHrH4Uv3HObwmv7wTbDKc5LJjyh
8, 36 -- lshg9j9NJvFCWkXAoQVWkfgwQstFT2RMaSOtadF26E7noZ5qHYbAnqAA8mMki/z4wO3z
8, 36 -- pMHRGvGro6Z7HOtAvDIjKwEq2sAw3cHAY4XDU=
8, 36 -- MIME-Version: 1.0
8, 36 -- Received: by 10.141.115.20 with SMTP id s20mr1251060rvm.285.1239983135072;
8, 36 -- Fri, 17 Apr 2009 08:45:35 -0700 (PDT)
8, 36 -- In-Reply-To: {c0bfda950904170842s1f651230w874d8d367d997508-at-mail.gmail.com}
8, 36 -- References: {200904171253.n3HCreNn011050-at-ns.microscopy.com}
8, 36 -- {c0bfda950904170842s1f651230w874d8d367d997508-at-mail.gmail.com}
8, 36 -- From: ID {ian.drucker-at-gmail.com}
8, 36 -- Date: Fri, 17 Apr 2009 09:45:20 -0600
8, 36 -- Message-ID: {c0bfda950904170845o37d71e4bn2ec979e23f72814b-at-mail.gmail.com}
8, 36 -- Subject: Re: [Microscopy] Need very large chamber environmental SEM with EDS
8, 36 -- To: Microscopy-at-microscopy.com
8, 36 -- Content-Type: text/plain; charset=ISO-8859-1
8, 36 -- Content-Transfer-Encoding: 8bit
8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3HFjZFq030514
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Fri, 17 Apr 2009 15:11:20 -0500
Subject: [Microscopy] Biowave DFR-10 owner's manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

I have inherited a Biowave DFR-10 (a fancy Ted Pella microwave) but I did not inherit the owner's manual.

Does anyone have a copy that they can send me?

Thanks and have a nice weekend.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
10, 26 -- From vapatpxs-at-yahoo.com Fri Apr 17 15:11:19 2009
10, 26 -- Received: from n13b.bullet.mail.mud.yahoo.com (n13b.bullet.mail.mud.yahoo.com [68.142.207.222])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3HKBJhF023860
10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 15:11:19 -0500
10, 26 -- Received: from [68.142.194.244] by n13.bullet.mail.mud.yahoo.com with NNFMP; 17 Apr 2009 20:11:19 -0000
10, 26 -- Received: from [68.142.201.68] by t2.bullet.mud.yahoo.com with NNFMP; 17 Apr 2009 20:11:18 -0000
10, 26 -- Received: from [127.0.0.1] by omp420.mail.mud.yahoo.com with NNFMP; 17 Apr 2009 20:11:18 -0000
10, 26 -- X-Yahoo-Newman-Property: ymail-3
10, 26 -- X-Yahoo-Newman-Id: 975762.72883.bm-at-omp420.mail.mud.yahoo.com
10, 26 -- Received: (qmail 68078 invoked by uid 60001); 17 Apr 2009 20:11:18 -0000
10, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1239999078; bh=cKUjnl04FOOTsenVt6TJydbwVrzOZj7hHXjGnJBOUY4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=nOkEWSy2UbSlCHU80nScUSlt7NoBqCtXExj6RjeckHjAFCMreenzIlxt9lto2zPJoEF+ZcA/F653H0E5upOpftEke9IXtffM/v6lLnP4/z/493t76CxV7eiGmvJJmkt9xNYkEPKE17CH+Od1iyjGXkgUCMIPNFOgFChm8YeggUk=
10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 26 -- s=s1024; d=yahoo.com;
10, 26 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
10, 26 -- b=SsKOFYDBpf6mDZOuCPR4jWBqR+hBjqoxeOwMKBHB+pGHsyeaMyLOS9ASjOY6ZDHi69nJFqqKuzs3hjL6vbQoGnZtpPMU5gj8sj2bheCCmEbNvvIVjT8Mel1sKeKXqoQfpYbKwbFZPFqXzV5riEt1NO+SrRtdBu5AuZR8ibnoChU=;
10, 26 -- Message-ID: {374878.67140.qm-at-web46110.mail.sp1.yahoo.com}
10, 26 -- X-YMail-OSG: Xr3bu2MVM1nJT5AwtSHm_dfNvnrHIPqcIRv1tMR6Wo8liqjq35CCF2c0SYvrb8CjXCJVO_0is3eprXlfFTNhSU3c5JbqOIPjl8yqPwC8Dgfp49nhgb418lDyUdZwVxU6tyHCHrVABF5kNurLsEg4PDRrSQURP_T0GDR2VNP5SkJBDtyJJ5vvVQXvuQL2adUFlRGjwMt1UD5rqfbGysylUTqaGK1AxS6ea2nR1.0YdcNsTf7PSvzHhiiwBbHg7n_Q2qzSuYtw8ScV57r4CA0-
10, 26 -- Received: from [132.239.85.200] by web46110.mail.sp1.yahoo.com via HTTP; Fri, 17 Apr 2009 13:11:18 PDT
10, 26 -- X-Mailer: YahooMailClassic/5.2.18 YahooMailWebService/0.7.289.1
10, 26 -- Date: Fri, 17 Apr 2009 13:11:18 -0700 (PDT)
10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
10, 26 -- Subject: Biowave DFR-10 owner's manual
10, 26 -- To: MSA BB {Microscopy-at-microscopy.com} ,
10, 26 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
10, 26 -- MIME-Version: 1.0
10, 26 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: gas19-at-chrysler.com
Date: Sat, 18 Apr 2009 08:10:42 -0500
Subject: [Microscopy] viaWWW: Re: Need very large chamber environmental SEM with EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gas19-at-chrysler.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gas19-at-chrysler.com
Name: Gerald Shulke

Organization: Chrysler LLC

Title-Subject: [Filtered] Re: [Microscopy] Need very large chamber
environmental SEM with EDS

Question: I think that you will find most of the major manufacturers
will have an ESEM that can accommodate large samples: Hitachi, JEOL,
Tescan, FEI, and Zeiss. X and Y stage travel will most likely not be
able to accommodate the total limits of your sample, but you will be
able to reposition your sample on the stage to get across your
sample. I think your limiting factor will be the height of the
sample, and being able to do EDS. 6 inches is pretty tall. I think
you can rule out several manufacturers based on this alone. Most
ESEMs have a small WD for EDS, which will work to your advantage. If
you cannot cut your sample and must have 6 inches of height, then you
are very limited. I don't have any experience with the scopes that
might accommodate that height. There seems to be a couple that may,
but you need to ask the right questions to them to see if EDS on a 6
inch cube is truly possible. If you are able to cut the sample, say
in half, then you've opened the door for just about all of the
manufacturers. Now you can compare all of the features of the ESEMs,
not just the height limitation.

Gerald Shulke


Login Host: 129.9.163.106
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Sat Apr 18 08:10:41 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3IDAflj006708
8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 18 Apr 2009 08:10:41 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c60f81af56b1-at-[206.69.208.22]}
8, 11 -- Date: Sat, 18 Apr 2009 08:10:40 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: gas19-at-chrysler.com (by way of Nestor J. Zaluzec)
8, 11 -- Subject: viaWWW: Re: Need very large chamber environmental SEM with EDS
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cvierret-at-mst.edu
Date: Sat, 18 Apr 2009 08:11:27 -0500
Subject: [Microscopy] viaWWW: Spring Meeting CSMMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cvierret-at-mst.edu
Name: Clarissa Wisner

Organization: Central States Microscopy and Microanalysis Society

Title-Subject: [Filtered] Spring Meeting

Question: Central States Microscopy and
Microanalysis Society wish to invite you to their
spring meeting in Columbia Mo.
The theme is Scince is Art. For more information
or to register please contact Clarissa Wisner,
cvierret-at-mst.edu.

Program includes a wide variety of speakers:

Kristy Wendt, Wash U Research Engineer - National
Nanotechnology Infrastructure Network

Dr. Paul Mainwaring, Gatan Inc. - X-ray
Microscopy and Tomography of Low Z Materials in
the SEM

Dr. Adrienne Hoard, Professor of Design and Color
Theory from MU - Color Theory in Imaging

Dr. Jay Jerome Department of Pathology,
Vanderbilt University Medical Center - Putting
the Art in Microscopy Images While Keeping the
Science

Dr. Ye Duan, Assistant Professor of Computer Science at MU
3D Computer Imaging in Science and Medicine

Dr. Gary Greenberg Author of A Grain of Sand:
Natureís Secret Wonder - The Art of Science: From
Pythagoras to the Present


Login Host: 131.151.25.189
---------------------------------------------------------------------------


==============================Original Headers==============================
15, 13 -- From zaluzec-at-microscopy.com Sat Apr 18 08:11:27 2009
15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3IDBQrZ007065
15, 13 -- for {microscopy-at-microscopy.com} ; Sat, 18 Apr 2009 08:11:27 -0500
15, 13 -- Mime-Version: 1.0
15, 13 -- Message-Id: {p06240801c60f81da60ac-at-[206.69.208.22]}
15, 13 -- Date: Sat, 18 Apr 2009 08:11:25 -0500
15, 13 -- To: microscopy-at-microscopy.com
15, 13 -- From: cvierret-at-mst.edu (by way of MicroscopyListserver)
15, 13 -- Subject: viaWWW: Spring Meeting CSMMS
15, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
15, 13 -- Content-Transfer-Encoding: 8bit
15, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3IDBQrZ007065
==============================End of - Headers==============================




From: lolitageorge-at-gmail.com
Date: Sat, 18 Apr 2009 08:11:52 -0500
Subject: [Microscopy] viaWWW: Total RNA extraction from glutaraldehyde fixed tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lolitageorge-at-gmail.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: lolitageorge-at-gmail.com
Name: Lolita

Title-Subject: [Filtered] Total RNA extraction from glutaraldehyde
fixed tissues...

Question: Can we efficiently extract total RNA from samples fixed
using sod.cacodylate buffer and Glutaraldehyde? I would like to do
some Real time PCR analysis and need some total RNA from the same
sections where I plan to do EM studies.

Please let me know your wise inputs.
Thanks

Login Host: 199.133.17.232
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Sat Apr 18 08:11:52 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3IDBpxv007637
6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 18 Apr 2009 08:11:52 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c60f82026a25-at-[206.69.208.22]}
6, 11 -- Date: Sat, 18 Apr 2009 08:11:50 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: lolitageorge-at-gmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Total RNA extraction from glutaraldehyde fixed tissues
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tomxlawson-at-gmail.com
Date: Sat, 18 Apr 2009 20:32:03 -0500
Subject: [Microscopy] Fluorescent Microcopy: Trying to locate some commercial easy-to-use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

I am researching the use of time-gated luminescence microscopy to
minimise autofluorescence.

The research team I work with makes its own lanthanide organometallic
fluorophores Eu3+ and Tb3+ which last about 3ms. But I am now getting
specimens from collaborators who sometimes want to label the specimens
at their end. Unfortunately lanthanides are not easy to use and
require UV excitation. So I am trying to find some easy-to-use
commercial long lifetime fluorophores. A lifetime of 1000ns would be
great. I have had some excellent suggestions so far such as:

1. Organometallic transition metal-ligand complexes such as
ruthenium (Ru II), rhenium (Re I), or osmium (Os II) with one or more
diimine ligands (10 ns to 10 ìs).
2. Organic fluorophores such as Pyrene (400ns) or Coronene (200ns).
3. Quantum dots.
4. Triplet probes such as eosin or erythrosin.

But I am having trouble sourcing the correct probes and I am not sure
how easy they are to use. I would be grateful if you could tell me
about any commercial easy-to-use around lifetime fluorophores you have
used.

Regards,

--
Tom Lawson
tomxlawson-at-gmail.com
PhD Student
Dept. of Physics
Macquarie University
NSW, 2109
Australia


==============================Original Headers==============================
8, 33 -- From tomxlawson-at-gmail.com Sat Apr 18 20:32:03 2009
8, 33 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.168])
8, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3J1W3N0005847
8, 33 -- for {Microscopy-at-Microscopy.Com} ; Sat, 18 Apr 2009 20:32:03 -0500
8, 33 -- Received: by wf-out-1314.google.com with SMTP id 28so1269703wfa.21
8, 33 -- for {Microscopy-at-Microscopy.Com} ; Sat, 18 Apr 2009 18:32:02 -0700 (PDT)
8, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 33 -- d=gmail.com; s=gamma;
8, 33 -- h=domainkey-signature:mime-version:received:date:message-id:subject
8, 33 -- :from:to:content-type:content-transfer-encoding;
8, 33 -- bh=5xnL5ebRa2tVjfVKZotI6+VqSCytRwzvNQ1uI8UDq14=;
8, 33 -- b=CYISkCoGknHJ0UXKU6JqB92PCOGGbk6PJo7mdwl6rGryLvE+ciOx8HJm7MQFxgqSET
8, 33 -- lB1bq9/GY+Xj7lHBQ4nZFucsSv8HTArjhiaO++HmcOsTxAqWNswA3qHeyJktoonvXDOm
8, 33 -- IenM6AXKzU/FSCGTaA2yUJAC+VAjEqbe6qrfM=
8, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 33 -- d=gmail.com; s=gamma;
8, 33 -- h=mime-version:date:message-id:subject:from:to:content-type
8, 33 -- :content-transfer-encoding;
8, 33 -- b=kRII9DNlY/HbyyQ9wPmYU283akNcyr1xq7h+98NwwKEcfbeWstna2Zuz7t4jZdqjxj
8, 33 -- btYpG1eA+wnI9SwuRK8SH5Xoc9gbBk+MAzIeF/x6Ng7qxZU/K6Aj/axmJ1MURF2JopwT
8, 33 -- rHKbhhKZLtBxD36zghRksbKs7rzvNycd5bZQk=
8, 33 -- MIME-Version: 1.0
8, 33 -- Received: by 10.142.139.14 with SMTP id m14mr764961wfd.244.1240104722579; Sat,
8, 33 -- 18 Apr 2009 18:32:02 -0700 (PDT)
8, 33 -- Date: Sun, 19 Apr 2009 11:32:02 +1000
8, 33 -- Message-ID: {61690070904181832k6ff5e34fke033e62b2830760d-at-mail.gmail.com}
8, 33 -- Subject: Fluorescent Microcopy: Trying to locate some commercial easy-to-use
8, 33 -- long lifetime fluorophores
8, 33 -- From: Tom Lawson {tomxlawson-at-gmail.com}
8, 33 -- To: Microscopy-at-Microscopy.Com
8, 33 -- Content-Type: text/plain; charset=ISO-8859-7
8, 33 -- Content-Transfer-Encoding: 8bit
8, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3J1W3N0005847
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Mon, 20 Apr 2009 09:24:39 -0500
Subject: [Microscopy] Meeting Announcement: Midwest Microscopy and Microanalysis Society Optical Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All,

The Midwest Microscopy and Microanalysis Society will hold its third
annual Optical Techniques Workshop on Thursday, May 28, 2009. This
year's program on basics of polarized light microscopy will include
lectures, applications demonstrations and hands-on training. Details
and registration information can be found on our website under Meetings:

www.midwestmicroscopy.org

Space for the hands-on portion of the program will be limited, so please
RSVP early if you plan to attend. We look forward to seeing you there.

Regards,

Elaine Schumacher
M3S Program Chair


*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com}
Web Site: www.mccrone.com {http://www.mccrone.com/}



*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************



==============================Original Headers==============================
12, 23 -- From eschumacher-at-mccrone.com Mon Apr 20 09:24:39 2009
12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3KEOcmZ014568
12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Apr 2009 09:24:38 -0500
12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959);
12, 23 -- Mon, 20 Apr 2009 09:23:24 -0500
12, 23 -- Content-class: urn:content-classes:message
12, 23 -- MIME-Version: 1.0
12, 23 -- Content-Type: text/plain;
12, 23 -- charset="US-ASCII"
12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society Optical Workshop
12, 23 -- Date: Mon, 20 Apr 2009 09:24:25 -0500
12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7021320CD-at-MCCRONEMSG.tmg.mccrone.com}
12, 23 -- X-MS-Has-Attach:
12, 23 -- X-MS-TNEF-Correlator:
12, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society Optical Workshop
12, 23 -- Thread-Index: AcnBw7TQXcP5ZctZR2ywQUD/d+l1dw==
12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
12, 23 -- To: {microscopy-at-microscopy.com}
12, 23 -- X-OriginalArrivalTime: 20 Apr 2009 14:23:24.0528 (UTC) FILETIME=[9095A700:01C9C1C3]
12, 23 -- Content-Transfer-Encoding: 8bit
12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3KEOcmZ014568
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Mon, 20 Apr 2009 18:07:44 -0500
Subject: [Microscopy] OptiQuip Model 1200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone,

In my continuing quest to find out how to finish putting the bulb assembly back together on my fluorescence microscope I've found out some additional information.

The power supply and bulb assembly were made by a company called "OptiQuip". I have called the company, which still exists, but have yet to receive a return phone call.

Anyway, if you have such a beasty and have put the bulb assembly back together, it holds either a xenon or mercury bulb, please let me know.

Even OptiQuip's owner's manual does not have a drawing or explain it clearly enough for me to understand.

Once more with feeling,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
12, 26 -- From vapatpxs-at-yahoo.com Mon Apr 20 18:07:43 2009
12, 26 -- Received: from n70.bullet.mail.sp1.yahoo.com (n70.bullet.mail.sp1.yahoo.com [98.136.44.38])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3KN7fxI014910
12, 26 -- for {microscopy-at-microscopy.com} ; Mon, 20 Apr 2009 18:07:42 -0500
12, 26 -- Received: from [216.252.122.217] by n70.bullet.mail.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:40 -0000
12, 26 -- Received: from [69.147.65.167] by t2.bullet.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:39 -0000
12, 26 -- Received: from [127.0.0.1] by omp502.mail.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:39 -0000
12, 26 -- X-Yahoo-Newman-Property: ymail-3
12, 26 -- X-Yahoo-Newman-Id: 962496.36827.bm-at-omp502.mail.sp1.yahoo.com
12, 26 -- Received: (qmail 76532 invoked by uid 60001); 20 Apr 2009 23:07:39 -0000
12, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1240268859; bh=4tHCA3MTjEc9qgiGOPpeM9AC7fBiwjr4kDFgU9hDsoE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=5AbqQohyAsRNUD2EuEy00KVX6y/Sh1jL4XoZnsax/jE2JVxO/Nyn7Uf5Q7EyiQlkFApQ+q2aH7HJnzLHwD2u7wSPJfJRdD25NY5mmT3woLslH5Bwt/P2HGS6EaBCL7KOBspWXDvFMDE7rZ1i6/xwrS3ZC65MBoS9UgAV7WsuU1M=
12, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 26 -- s=s1024; d=yahoo.com;
12, 26 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 26 -- b=G9Qa/PqpXTLFeHulLcuc7U8IW1+pOXeS1E5GLleOYz2rEhFn542jhzg0q2tXsldXnRSiGRBIlX6gCggYLSJoZiJNA/yiYzFqnrqL3fcybA1CLcZI/iQZNP9O7WIkFd1sNxeP7GjWXmFAG70EPQ/rdghm5TK+evW5AYjjxwUMPbA=;
12, 26 -- Message-ID: {801623.76495.qm-at-web46101.mail.sp1.yahoo.com}
12, 26 -- X-YMail-OSG: 8ICPzd0VM1kGehoYPWzq5LxJ815hL57J_W5ApI73Iwu8JYF6tUnZsXGIm9lDaPsKQXmjA.ApiIrpHcEH_AMs79cg1i43qs.ew5morVK7DEaecIGzervlhdw.TEsxCK3628Sq6l8w75y2BvWPbaQwl8audKe3af9bYOhTrgV0oIC4cVzL_4y034rA3Kox5JUegh_cHRTRlCGJuH85gZxkMdGvnUjqWBLc5609unafgiP3QEEvpG0XhQTDrGWTgiUbLjQA6IMlpIaTYwloipI-
12, 26 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Mon, 20 Apr 2009 16:07:37 PDT
12, 26 -- X-Mailer: YahooMailClassic/5.2.18 YahooMailWebService/0.7.289.1
12, 26 -- Date: Mon, 20 Apr 2009 16:07:37 -0700 (PDT)
12, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 26 -- Subject: OptiQuip Model 1200
12, 26 -- To: MSA BB {Microscopy-at-microscopy.com} ,
12, 26 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
12, 26 -- MIME-Version: 1.0
12, 26 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: ademp-at-mse.ufl.edu
Date: Mon, 20 Apr 2009 19:30:18 -0500
Subject: [Microscopy] viaWWW: Certificate in Materials Characterization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ademp-at-mse.ufl.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ademp-at-mse.ufl.edu
Name: L. Amelia Dempere

Organization: University of Florida

Title-Subject: [Filtered] Certificate in Materials Characterization

Question: This 9-credit online certificate is an opportunity for
multidisciplinary scientists and engineers
with various educational backgrounds to learn more about current
materials characterization techniques.
The certificate program is open to all scientists and
engineers, with various educational backgrounds.
The 3-credit course "Scanning Electron Microscopy and Microanalysis"
is offered this summer. The course is designed to introduce the
theoretical
concepts of Scanning Electron Microscopy (SEM),
Electron Probe Microanalysis (EPMA), and Energy
Dispersive Spectroscopy (EDS).
Additional information at: www.ufedge.eng.ufl.edu


Login Host: 128.227.78.197
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Apr 20 19:30:18 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3L0UG3e030930
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 20 Apr 2009 19:30:18 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c612c30dac68-at-[206.69.208.22]}
7, 11 -- Date: Mon, 20 Apr 2009 19:30:13 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: ademp-at-mse.ufl.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Certificate in Materials Characterization
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 21 Apr 2009 04:24:04 -0500
Subject: [Microscopy] RE: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree that 100dpi is perfectly fine for poster printing of most images
where detail is difficult to discern anyway, say fluorescence in cells or
tissues. You do notice low resolution jaggies in the text and in things we
are good at discerning, say a microscope, illustrator image or lab view
[when we know what they should look like]. Blurred printing on cheap paper
[the standard for most poster printing] means that the 600dpi of a modern
printer is unlikely ever to be realised. So although we easily spend £2,000+
in man hours producing a poster, we rarely spend more than £40 printing it
[and for most purposes this poster resolution is perfectly adequate anyway].
Plus the inks used may be rubbish, our University printed posters have all
faded in a month or two after being left up in a windowless lab [obviously
not HP inks and HP photographic paper where the inks are guaranteed fast for
100 years]. Fine for posters you use once and bin, but a pain for our
constantly re-used & recycled Core Facility poster.

However I would still advise against going to lower than say 1,000x750 for
an image whatever size it's going to be printed, if you think you might
possibly use it again. Our Core poster has 40+ images going back 5 years,
and the 'master' hi-res images are simply lost to history. Probably they
were deleted from my processors personal hard drive space when he left, and
probably they weren't all ours, but from our collaborators/users. So it is
an incredible pain to find that all our images on our main poster ppt files
are about 250x300 max pixel size - with multiple backups at different
locations, it's often only the poster master ppt file itself that survives.
If, at a later date, I want to increase the printed image size from 2x1.5"
to say 6x4.5" the pixilation is very noticeable. Plus they look bad in Core
PowerPoint and pdf presentations - and we are supposed to be the kings of
imaging. You can get by, say by upscaling and re-adding text at higher res -
but what a pain, more hours in Photoshop, and with typical day rates of
about £400+. And the extra file size of the larger images in the original
ppt poster file would have been insignificant on a modern PC.

Keeping track of digital images for the next 30 years is just about
impossible, if I want an image from one of my old papers these days I have
scan the printed copy. I guess I produce 10,000+ digital images a year at
home and at work these days. Backup is one thing, finding a particular image
again quickly years on is another matter.

Regards

Keith


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.


Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: 16 April 2009 15:39
To: kjmorris-at-well.ox.ac.uk

Our school is using PowerPoint as default software for creating posters for
many years.

While 300 ppi is a "golden standard" for printers (glossy magazines), it is
surely overkill for posters printed with inkjet printers. From my experience
printing images from XL30 SEM (about 1400*1000 pixels) at 11" image size
(i.e. 127 ppi) produce good results. Even 100 ppi on poster could be good
enough. But for line drawings you need higher resolution.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: dkloos-at-parallaxray.com [mailto:dkloos-at-parallaxray.com]
} Sent: Tuesday, April 14, 2009 12:57 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I just took my 'slides' to Kinko's and had them printed up to
} desired size.
} Worked great.
}
} Don Kloos
} VP Sales, Marketing, Business Development Parallax Research, Inc.
}
}
}
} Sales & Marketing
} 16478 Beach Blvd. #330
} Westminster, California, 92683-7860 USA
}
} TOLL FREE 1 866 581-XRAY (9729)
} Telephone 1 714 897-9779
} Fax 1 714 897-1421
} Email: dkloos-at-parallaxray.com
} SKYPE: don.kloos
} Website: http://www.parallaxray.com
}
}
}
} -----Original Message-----
} X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
} Sent: Tuesday, April 14, 2009 9:11 AM
} To: dkloos-at-parallaxray.com
} Subject: [Microscopy] RE: Advice: Resolution of micrographs
} for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} HI Kristen
} There is lots to recommend Power point but posters isn't its strongest
} suite.
} Our media specialist tells me the Power Point's output size
} is one of its
} weak points
} Poster size width is determined by the printer being used
} (our is 43 inches)
} while the maximum length is 56 inches
} Lengths greater than 56 leave the printer scratching its head
} though there
} are work arounds.
}
} Rick,
}
} Richard Harris, Manager - Imaging and Data Systems
} The Biotron - Experimental Climate Change Research
} University of Western Ontario,
} London Ontario, CANADA.
} N6A 5B7
} Ph.  519-661-2111 ext. 86780
} Fax  519-661-3935
} e-mail rjharris-at-uwo.ca
} web: www.biotron.uwo.ca
}
}
} -----Original Message-----
} X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com]
} Sent: Monday, April 13, 2009 6:19 PM
} To: rjharris-at-uwo.ca
} Subject: [Microscopy] Advice: Resolution of micrographs for posters?
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
}
} Hi All,
}
} I'm getting ready to "teach" my undergraduate EM class how to create a
} poster presentation of their work and I need a little
} educating as well.
} Could you please send me your sage advice on creating a
} poster in PowerPoint
} (that's what we are required to use) regarding the resolution of the
} electron micrographs? As background, we are developing TEM
} film, scanning
} the negatives, and manipulating them with Adobe Photoshop.
}
} Thanks,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Lecturer, Department of Biology
} Frostburg State University
}
} kalennon-at-frostburg.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009
} 8, 20 -- Received: from web84001.mail.mud.yahoo.com
} (web84001.mail.mud.yahoo.com [68.142.206.171])
} 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id
} n3DME1fS026918
} 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr
} 2009 17:14:01
} -0500
} 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr
} 2009 22:14:01
} -0000
} 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256;
} c=relaxed/relaxed; d=yahoo.com;
} s=s1024; t=1239660840;
} bh=Hr4c8e07wXoHDmP5Vlr+0Nro1sSEP2MofzkAUoG+LFo=;
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
o:MIME-Version
} :Content-Type;
} b=QdDLdklkKjl/vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/5pfJcQHaRTqz
} v0F9Du06x80gL9
} WfK9tfgY5blF/Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3W
} Ril6P7zxv9Mv99
} ILd8f0frdvPOc7pH66pXM=
} 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 8, 20 -- s=s1024; d=yahoo.com;
} 8, 20 --
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T
o:MIME-Version
} :Content-Type;
} 8, 20 --
} b=CRJXqaYio8A65tWLDZzLUty0rsYt37M+1zmFke8nyQmsSxP5eSw3IP902oyf
57dVuhsdE69j2m
} HCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt+9ZB+ajEXgx
7b+Mpe2yAzlxL1
} 1rki3h2FKA/pqZL+/La5Y=;
} 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com}
} 8, 20 -- X-YMail-OSG:
} GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48Ldabp
} A7uxHRhCirAh.4
} WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtng
} fec2j2sPU.vV3Y
} CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw
} 6Kks1l4MQ2VXbx
} ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LL
zFcha2HwyW_LQt
} DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA--
} 8, 20 -- Received: from [96.239.150.112] by
} web84001.mail.mud.yahoo.com via
} HTTP; Mon, 13 Apr 2009 15:14:00 PDT
} 8, 20 -- X-Mailer: YahooMailClassic/5.2.15
} YahooMailWebService/0.7.289.1
} 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT)
} 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com}
} 8, 20 -- Subject: Advice: Resolution of micrographs for posters?
} 8, 20 -- To: microscopy-at-microscopy.com
} 8, 20 -- MIME-Version: 1.0
} 8, 20 -- Content-Type: text/plain; charset=us-ascii
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 19, 29 -- From rjharris-at-uwo.ca Tue Apr 14 10:58:49 2009
} 19, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca
} [129.100.74.146])
} 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id
} n3EFwmH3008207
} 19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr
} 2009 10:58:49
} -0500
} 19, 29 -- MIME-version: 1.0
} 19, 29 -- Content-type: text/plain; charset=iso-8859-1
} 19, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri
} [172.29.32.39])
} 19, 29 -- by harpo.mail.uwo.pri
} 19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04
} (built Sep 26
} 2008; 32bit))
} 19, 29 -- with ESMTP id {0KI300L1FLPZ0Y00-at-harpo.mail.uwo.pri} for
} 19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009
} 11:58:47 -0400 (EDT)
} 19, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca
} [129.100.52.17])
} 19, 29 -- by harpo.mail.uwo.pri
} 19, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04
} (built Sep 26
} 2008; 32bit))
} 19, 29 -- with ESMTPSA id {0KI300K3ZLPZEI00-at-harpo.mail.uwo.pri} for
} 19, 29 -- Microscopy-at-microscopy.com; Tue, 14 Apr 2009
} 11:58:47 -0400 (EDT)
} 19, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
} 19, 29 -- To: kamlennon-at-yahoo.com
} 19, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
} 19, 29 -- References: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
} 19, 29 -- In-reply-to: {200904132219.n3DMJTnn006591-at-ns.microscopy.com}
} 19, 29 -- Subject: RE: [Microscopy] Advice: Resolution of
} micrographs for
} posters?
} 19, 29 -- Date: Tue, 14 Apr 2009 11:58:47 -0400
} 19, 29 -- Message-id: {00f601c9bd19$e5888010$b0998030$-at-ca}
} 19, 29 -- X-Mailer: Microsoft Office Outlook 12.0
} 19, 29 -- Thread-index: Acm8hexsPjIfYHJqSt6Tn+qeWbWeggAk10Nw
} 19, 29 -- Content-language: en-us
} 19, 29 -- Content-Transfer-Encoding: 8bit
} 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n3EFwmH3008207
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 29, 31 -- From dkloos-at-parallaxray.com Tue Apr 14 12:56:32 2009
} 29, 31 -- Received: from cp18.heritagewebdesign.com
} (cp18.heritagewebdesign.com [209.90.77.54])
} 29, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n3EHuVqD014372
} 29, 31 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr
} 2009 12:56:31 -0500
} 29, 31 -- Received: from user-0c8gg59.cable.mindspring.com
} ([24.136.64.169] helo=donl)
} 29, 31 -- by cp18.heritagewebdesign.com with esmtpa (Exim
} 4.69 (FreeBSD))
} 29, 31 -- (envelope-from {dkloos-at-parallaxray.com} )
} 29, 31 -- id 1Ltms8-00034B-Nl; Tue, 14 Apr 2009 11:56:36 -0600
} 29, 31 -- Reply-To: {dkloos-at-parallaxray.com}
} 29, 31 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
} 29, 31 -- To: {microscopy-at-microscopy.com}
} 29, 31 -- Cc: {rjharris-at-uwo.ca}
} 29, 31 -- References: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
} 29, 31 -- Subject: RE: [Microscopy] RE: Advice: Resolution of
} micrographs for posters?
} 29, 31 -- Date: Tue, 14 Apr 2009 10:56:24 -0700
} 29, 31 -- Organization: Parallax Research
} 29, 31 -- Message-ID: {2B5B9B340BD2412FB23D2C53AF29F19E-at-donl}
} 29, 31 -- MIME-Version: 1.0
} 29, 31 -- Content-Type: text/plain;
} 29, 31 -- charset="iso-8859-1"
} 29, 31 -- X-Mailer: Microsoft Office Outlook 11
} 29, 31 -- In-Reply-To: {200904141611.n3EGBSfQ021422-at-ns.microscopy.com}
} 29, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 29, 31 -- Thread-Index: Acm9G64h6c8cFDLwQ/+utZ/f7mH7TwADom1A
} 29, 31 -- X-AntiAbuse: This header was added to track abuse,
} please include it with any abuse report
} 29, 31 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
} 29, 31 -- X-AntiAbuse: Original Domain - microscopy.com
} 29, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
} 29, 31 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
} 29, 31 -- Content-Transfer-Encoding: 8bit
} 29, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit
} by ns.microscopy.com id n3EHuVqD014372
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Thu Apr 16 09:30:21 2009
8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu
[134.193.143.167])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3GEUKSv013479
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 09:30:21
-0500
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Thu, 16 Apr 2009 09:30:20 -0500
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="iso-8859-1"
8, 25 -- Subject: RE: [Microscopy] Advice: Resolution of micrographs for
posters?
8, 25 -- Date: Thu, 16 Apr 2009 09:30:20 -0500
8, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB825-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200904141757.n3EHv4oA015903-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] Advice: Resolution of micrographs for
posters?
8, 25 -- Thread-Index: Acm9Kmz+bZXCpkekRKOXFcmy6Ymb4QBcowPg
8, 25 -- References: {200904141757.n3EHv4oA015903-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 16 Apr 2009 14:30:20.0490 (UTC)
FILETIME=[DEDD6AA0:01C9BE9F]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3GEUKSv013479
==============================End of - Headers==============================



==============================Original Headers==============================
23, 23 -- From kjmorris-at-well.ox.ac.uk Tue Apr 21 04:24:03 2009
23, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
23, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3L9O0R0030136
23, 23 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 04:24:01 -0500
23, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
23, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
23, 23 -- id 1LwCCs-00043G-Bf
23, 23 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 10:23:58 +0100
23, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
23, 23 -- To: {microscopy-at-microscopy.com}
23, 23 -- References: {200904161438.n3GEcj2v023869-at-ns.microscopy.com}
23, 23 -- Subject: RE: [Microscopy] RE: Advice: Resolution of micrographs for posters?
23, 23 -- Date: Tue, 21 Apr 2009 10:24:40 +0100
23, 23 -- Message-ID: {F3B724418EF44E9EAC015431C142DF3C-at-CytoWhizz}
23, 23 -- MIME-Version: 1.0
23, 23 -- Content-Type: text/plain;
23, 23 -- charset="iso-8859-1"
23, 23 -- X-Mailer: Microsoft Office Outlook 11
23, 23 -- Thread-Index: Acm/KAuKSlo8wW2IQXOzqZMfint0xwCptXgg
23, 23 -- In-Reply-To: {200904161438.n3GEcj2v023869-at-ns.microscopy.com}
23, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 23 -- Content-Transfer-Encoding: 8bit
23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3L9O0R0030136
==============================End of - Headers==============================




From: ben.micklem-at-pharm.ox.ac.uk
Date: Tue, 21 Apr 2009 06:14:47 -0500
Subject: [Microscopy] Re: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm sorry if this sounds harsh Keith, but it seems a little odd to me
that you use PowerPoint, designed for on-screen presentations, to
produce posters.

It is under £120 for you to buy InDesign CS4 (OUCS shop- license and
media), and it will save you time as you never have to re-make posters
for different sizes, and the process itself is a lot faster. You can
probably justify the cost in time savings after only one or two posters.

I know it doesn't address Kristen's question, as she is required to use
PowerPoint. For importing into PowerPoint (or any Office application),
PNG is the best file type, as it is the native compression for office.
I would tend to go for 300ppi, just because computers can cope with
large files, but realistically 210ppi is indistinguishable. Don't be
confused with printers' dpi (typically inkjet for this application), and
pixels per inch. Inkjets can not print continuous tone, they have to
place many dots to achieve the colour information contained in a single
pixel.



Now to preach on the alternative :)

InDesign links to the original full-resolution version of all images,
via the 'place' command, and you can work with thousands of images of
very large file sizes without it affecting the speed or reliability of
the programme (it is designed for typesetting whole books after all)-
this is because it only works with a low-resolution preview of the
original file. The InDesign files themselves are never over a few megabytes

You can 'package' all the linked images for a file together in the same
place (this duplicates all the linked files and places them together-
useful if you have linked to originals in multiple folders spread around
your computer).

When you want to have the poster printed, you export as a PDF,
down-sampling all the images to the desired resolution and compression
in one simple step. The result is a very robust file with all fonts
embedded and almost fool-proof for printing, that is never too large or
too small for the job.

If you want to edit an image in the poster, you don't have to find your
original high resolution file, and make changes, down-sample and
re-import, as you would have to do in PowerPoint. Instead, you just
right-click, and select 'edit original' from the contextual menu, and it
opens the orignal in Photoshop, and after making changes you close the
document, and the preview in InDesign will automatically update.

If you ever use vector files, PowerPoint is a nightmare; where as
InDesign links directly to Illustrator files, and changes are very
quick. And the PDFs created by InDesign will obviously contain perfect
copies of the vector information, so even if you do lose all version of
a diagram, except a low-resolution PDF of a A0 poster designed for
printing A4 as hand-outs, you can open the PDF in Illustrator and copy
the perfect diagram out of the file.

These are all in addition to the much more sophisticated text tools
(justified text with complete control over the balance between enlarged
spaces between words or letters, and hyphenation; word length before
hyphenation should occur), automatic alignment tools and the ability to
instantly spread items at equal spacing, or a pre-set spacing...

Kind regards,

Ben


--
Imaging Technician
MRC Anatomical Neuropharmacology Unit, Mansfield Road,
Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
{http://mrcanu.pharm.ox.ac.uk/}

kjmorris-at-well.ox.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I agree that 100dpi is perfectly fine for poster printing of most images
} where detail is difficult to discern anyway, say fluorescence in cells or
} tissues. You do notice low resolution jaggies in the text and in things we
} are good at discerning, say a microscope, illustrator image or lab view
} [when we know what they should look like]. Blurred printing on cheap paper
} [the standard for most poster printing] means that the 600dpi of a modern
} printer is unlikely ever to be realised. So although we easily spend £2,000+
} in man hours producing a poster, we rarely spend more than £40 printing it
} [and for most purposes this poster resolution is perfectly adequate anyway].
} Plus the inks used may be rubbish, our University printed posters have all
} faded in a month or two after being left up in a windowless lab [obviously
} not HP inks and HP photographic paper where the inks are guaranteed fast for
} 100 years]. Fine for posters you use once and bin, but a pain for our
} constantly re-used & recycled Core Facility poster.
}
} However I would still advise against going to lower than say 1,000x750 for
} an image whatever size it's going to be printed, if you think you might
} possibly use it again. Our Core poster has 40+ images going back 5 years,
} and the 'master' hi-res images are simply lost to history. Probably they
} were deleted from my processors personal hard drive space when he left, and
} probably they weren't all ours, but from our collaborators/users. So it is
} an incredible pain to find that all our images on our main poster ppt files
} are about 250x300 max pixel size - with multiple backups at different
} locations, it's often only the poster master ppt file itself that survives.
} If, at a later date, I want to increase the printed image size from 2x1.5"
} to say 6x4.5" the pixilation is very noticeable. Plus they look bad in Core
} PowerPoint and pdf presentations - and we are supposed to be the kings of
} imaging. You can get by, say by upscaling and re-adding text at higher res -
} but what a pain, more hours in Photoshop, and with typical day rates of
} about £400+. And the extra file size of the larger images in the original
} ppt poster file would have been insignificant on a modern PC.
}
} Keeping track of digital images for the next 30 years is just about
} impossible, if I want an image from one of my old papers these days I have
} scan the printed copy. I guess I produce 10,000+ digital images a year at
} home and at work these days. Backup is one thing, finding a particular image
} again quickly years on is another matter.
}
} Regards
}
} Keith
}
}
} ---------------------------------------------------------------------------
} Dr Keith J. Morris,
} Molecular Cytogenetics and Microscopy Core,
} Laboratory 00/069 and 00/070,
} The Wellcome Trust Centre for Human Genetics,
} Roosevelt Drive,
} Oxford OX3 7BN,
} United Kingdom.
}
}
} Telephone: +44 (0)1865 287568
} Email: kjmorris-at-well.ox.ac.uk
} Web-pages: http://www.well.ox.ac.uk/cytogenetics/

==============================Original Headers==============================
17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009
17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169])
17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LBEktC015545
17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 06:14:47 -0500
17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207])
17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
17, 28 -- id 1LwDw6-0002ch-UF
17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 +0100
17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
17, 28 -- (Exim 4.69)
17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
17, 28 -- id 1LwDw6-0002Oo-3F
17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 +0100
17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk}
17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100
17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
17, 28 -- MIME-Version: 1.0
17, 28 -- To: microscopy-at-microscopy.com
17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of micrographs for posters?
17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
17, 28 -- X-Enigmail-Version: 0.95.7
17, 28 -- Content-Type: text/plain; charset=ISO-8859-1
17, 28 -- Content-Transfer-Encoding: 8bit
17, 28 -- X-Oxford-Username: phar0293
==============================End of - Headers==============================




From: yvan_lindekens-at-yahoo.com
Date: Tue, 21 Apr 2009 08:09:59 -0500
Subject: [Microscopy] LM: Affordable microtome knife reconditioning in Europe?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have some large (25 - 30cm) type B, C and D, both steel and tungsten carbide, microtome knives that need sharpening/reconditioning.

Does anyone knows a company in Europe that does that kind of work at an affordable price? By the way: what is *normal* price for those things? Impossible to answer question, I suppose...

Thanks in advance!

Yvan.




==============================Original Headers==============================
8, 20 -- From yvan_lindekens-at-yahoo.com Tue Apr 21 08:09:58 2009
8, 20 -- Received: from web110214.mail.gq1.yahoo.com (web110214.mail.gq1.yahoo.com [67.195.8.190])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3LD9wfL001004
8, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 08:09:58 -0500
8, 20 -- Received: (qmail 88944 invoked by uid 60001); 21 Apr 2009 13:09:57 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1240319397; bh=c8NMl8oSSxu20/MV3gn/cthQ8KmQqO8ds9ffhGip1+s=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=vTus0tsjxXCZiDuO1gCtEfiOV794lNkgldgzfHNAiy9zMrwwjzmqJzvoJyT6BwI3rWsZ9nNJC1Y+RB5thVF4tmVavfXBgrTnhSE6LXl29O7KnIBsMefp98i1DOveEB6YxqCr7DmljrGgFvS6Bo7En0sgpOwsoKDmybaC3uGYwv0=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=oVK8x07C5YabFe7fN1S+scojIAbrIWlyrVuLZkcDBqPN2i0TXWj45AM2bq8r5UV+wwqz23pWI3t+MyG1sbMSUhKo88gjpSi2Hk/fnbTo1RFc/Sj0qzGw0ELyHxPUYyOIjpTGrvxHEQ1VxT9u6w8qj3idioN/0sGbvnAwNWN+QQU=;
8, 20 -- Message-ID: {711073.87315.qm-at-web110214.mail.gq1.yahoo.com}
8, 20 -- X-YMail-OSG: lZLQAB4VM1nHcw1l08kxZHBApXi47OJPpNIXEtnSSqzBJkudY91U1guOmIWEOjyHMjYOCzrj3E2Lm5eA82daUDabjM63fPoxLtiosCvRBl70rZcwmSKE9BRl__040fpn5cEr31UVOrgp68zj7Fo25n.UvcL_GMmGV1nrb4xhPnEId5wvnQhw_lBqdew1cyASI2.SkM2ibzw.vP4wbn.O6Ddc5oCStQnDKuiNemPcn7QlgYUheH4VVm3IWP8jqIUx4cE6mUSfcOikJe89MQBZxP_Esh8gYNnkMAMXvoJffW9olOkwfQ--
8, 20 -- Received: from [87.64.36.172] by web110214.mail.gq1.yahoo.com via HTTP; Tue, 21 Apr 2009 06:09:57 PDT
8, 20 -- X-Mailer: YahooMailWebService/0.7.289.1
8, 20 -- Date: Tue, 21 Apr 2009 06:09:57 -0700 (PDT)
8, 20 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com}
8, 20 -- Subject: LM: Affordable microtome knife reconditioning in Europe?
8, 20 -- To: Microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: yvan_lindekens-at-yahoo.com
Date: Tue, 21 Apr 2009 08:11:45 -0500
Subject: [Microscopy] LM: Honing plates for Reichert Microtome Knife Sharpener Model 903

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

It struck me that all those microtome knife sharpeners (AO 935, Reichert 903, AO 903...) all look very much the same. Are the glass plates interchangeable?

Would the glass plates for the current Leica sharpener SP9000 (part.no. 14041819698, dimensions 292mm x 127mm x 6 mm) fit the Reichert Microtome Knife Sharpener 903?

It would be great if someone owning a model 903 could measure the plates :-).

Thanks in advance for your answers!

Yvan.





==============================Original Headers==============================
10, 20 -- From yvan_lindekens-at-yahoo.com Tue Apr 21 08:11:44 2009
10, 20 -- Received: from web110204.mail.gq1.yahoo.com (web110204.mail.gq1.yahoo.com [67.195.8.180])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3LDBgLk002317
10, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 08:11:43 -0500
10, 20 -- Received: (qmail 79198 invoked by uid 60001); 21 Apr 2009 13:11:41 -0000
10, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1240319501; bh=8zyqYneQV0djNT8gRvnJVfs3RPETEkUejg2oIMK5f8g=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=ml1iIKCamZp9r/cVPFXkNC3hFh7h5tm7unwYXND3pDXTGDMBzuELsebV8cAl65GxxWlDSTjg6YuomH5ZmM8tN0b2CnnsfwMr6dop4RjdB0fUSNJgar+IkXuNW4kXbP0BEeQlojuirfMc89Lb1XdjJD+tFPEtGDk87gjDuWYC1IE=
10, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 20 -- s=s1024; d=yahoo.com;
10, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
10, 20 -- b=BtSyGs2wA8PNwT/tYjHaLgWaE5HO9jfr735yLraqddqxJq+FvzpZ5LrY/LCn77TiARG4TNA53ee1YQFtz2jDBNsTxkVgezdA1Wr11677uSBnpTszzoiuV7H3orPTnJEDMipiFN1BmJkU4SbfeOExU+ghTB1R1vY6E5rA0ANElU0=;
10, 20 -- Message-ID: {276395.78952.qm-at-web110204.mail.gq1.yahoo.com}
10, 20 -- X-YMail-OSG: 6khuMgsVM1leIeAIA5cpkAXeUPR_HfkCiHz4_t5JtzDKKLRGMBJFZ4r0IJSj9IZXNyXZU_0q9wM6iYQdNVLtX01Gx.je5YrQ75SgNoywk1WtdXKJg6Ov8VEzys_2JLy0qgGewrqLY3iZkIeyW5hdXQ0e3EnQpIJBjZpe36J7PFZzOrBuxvR5_Hoa8bxEIaH5.51gR2HBQL9nu2PjCkAx5it2Qq3y0_fkUDUS19Had6ENud.J.EeKGaeildDzmCMtCb5T.agGj8ntajFHK640FMsJIIYkabqmsWZulhauhndbtxtCyw--
10, 20 -- Received: from [87.64.36.172] by web110204.mail.gq1.yahoo.com via HTTP; Tue, 21 Apr 2009 06:11:41 PDT
10, 20 -- X-Mailer: YahooMailWebService/0.7.289.1
10, 20 -- Date: Tue, 21 Apr 2009 06:11:41 -0700 (PDT)
10, 20 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com}
10, 20 -- Subject: LM: Honing plates for Reichert Microtome Knife Sharpener Model 903
10, 20 -- To: Microscopy-at-microscopy.com
10, 20 -- MIME-Version: 1.0
10, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 21 Apr 2009 14:07:53 -0500
Subject: [Microscopy] RE: insurance - based contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks a lot for a lot of helpful answers.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Tue Apr 21 14:07:52 2009
7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LJ7o2u009264
7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 14:07:52 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Tue, 21 Apr 2009 14:07:34 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] insurance - based contracts
7, 25 -- Date: Tue, 21 Apr 2009 14:07:33 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB832-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB831-at-KC-MSX1.kc.umkc.edu}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] insurance - based contracts
7, 25 -- Thread-Index: Acm+r30Ejxic0NU0R26dm8NB76D/igEAVsYAAADgjMA=
7, 25 -- References: {200904161622.n3GGM6fW032497-at-ns.microscopy.com} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB831-at-KC-MSX1.kc.umkc.edu}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 21 Apr 2009 19:07:34.0367 (UTC) FILETIME=[6D7E5EF0:01C9C2B4]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3LJ7o2u009264
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Tue, 21 Apr 2009 16:17:33 -0500
Subject: [Microscopy] Fwd: Re: Advice: Resolution of micrographs for posters?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ben,

Just thought I'd let you know that your nice write up on the
advantages of InDesign has been appreciated! That does sound like the
right way to keep your posters and related stuff organized. One of
these days...

On the meetings I have been too, though, your InDesign-made would be
in a small minority, so please don't be too harsh on Keith... By far
the majority of those posters I've seen have been done in PowerPoint.
From that perspective, doing your poster in PP today is hardly "odd",
although it is of course not the optimal way.

Now back to being serious and constructive - this does sound like
something people who make posters should be more aware of. Perhaps we
could ask those Adobe representatives ("evangelists") to some
tutorials during microscopy meetings? (I am thinking about M&M
meetings in particular, but there are of course others...)

Vlad

________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.


} I'm sorry if this sounds harsh Keith, but it seems a little odd to me
} that you use PowerPoint, designed for on-screen presentations, to
} produce posters.
}
} It is under £120 for you to buy InDesign CS4 (OUCS shop- license and
} media), and it will save you time as you never have to re-make posters
} for different sizes, and the process itself is a lot faster. You can
} probably justify the cost in time savings after only one or two
} posters.
}
} I know it doesn't address Kristen's question, as she is required to
} use
} PowerPoint. For importing into PowerPoint (or any Office application),
} PNG is the best file type, as it is the native compression for
} office.
} I would tend to go for 300ppi, just because computers can cope with
} large files, but realistically 210ppi is indistinguishable. Don't be
} confused with printers' dpi (typically inkjet for this application),
} and
} pixels per inch. Inkjets can not print continuous tone, they have to
} place many dots to achieve the colour information contained in a
} single
} pixel.
}
}
}
} Now to preach on the alternative :)
}
} InDesign links to the original full-resolution version of all images,
} via the 'place' command, and you can work with thousands of images of
} very large file sizes without it affecting the speed or reliability of
} the programme (it is designed for typesetting whole books after all)-
} this is because it only works with a low-resolution preview of the
} original file. The InDesign files themselves are never over a few
} megabytes
}
} You can 'package' all the linked images for a file together in the
} same
} place (this duplicates all the linked files and places them together-
} useful if you have linked to originals in multiple folders spread
} around
} your computer).
}
} When you want to have the poster printed, you export as a PDF,
} down-sampling all the images to the desired resolution and compression
} in one simple step. The result is a very robust file with all fonts
} embedded and almost fool-proof for printing, that is never too large
} or
} too small for the job.
}
} If you want to edit an image in the poster, you don't have to find
} your
} original high resolution file, and make changes, down-sample and
} re-import, as you would have to do in PowerPoint. Instead, you just
} right-click, and select 'edit original' from the contextual menu,
} and it
} opens the orignal in Photoshop, and after making changes you close the
} document, and the preview in InDesign will automatically update.
}
} If you ever use vector files, PowerPoint is a nightmare; where as
} InDesign links directly to Illustrator files, and changes are very
} quick. And the PDFs created by InDesign will obviously contain perfect
} copies of the vector information, so even if you do lose all version
} of
} a diagram, except a low-resolution PDF of a A0 poster designed for
} printing A4 as hand-outs, you can open the PDF in Illustrator and copy
} the perfect diagram out of the file.
}
} These are all in addition to the much more sophisticated text tools
} (justified text with complete control over the balance between
} enlarged
} spaces between words or letters, and hyphenation; word length before
} hyphenation should occur), automatic alignment tools and the ability
} to
} instantly spread items at equal spacing, or a pre-set spacing...
}
} Kind regards,
}
} Ben
}
}
} --
} Imaging Technician
} MRC Anatomical Neuropharmacology Unit, Mansfield Road,
} Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
} {http://mrcanu.pharm.ox.ac.uk/}
}
} kjmorris-at-well.ox.ac.uk wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I agree that 100dpi is perfectly fine for poster printing of most
} } images
} } where detail is difficult to discern anyway, say fluorescence in
} } cells or
} } tissues. You do notice low resolution jaggies in the text and in
} } things we
} } are good at discerning, say a microscope, illustrator image or lab
} } view
} } [when we know what they should look like]. Blurred printing on
} } cheap paper
} } [the standard for most poster printing] means that the 600dpi of a
} } modern
} } printer is unlikely ever to be realised. So although we easily
} } spend £2,000+
} } in man hours producing a poster, we rarely spend more than £40
} } printing it
} } [and for most purposes this poster resolution is perfectly adequate
} } anyway].
} } Plus the inks used may be rubbish, our University printed posters
} } have all
} } faded in a month or two after being left up in a windowless lab
} } [obviously
} } not HP inks and HP photographic paper where the inks are guaranteed
} } fast for
} } 100 years]. Fine for posters you use once and bin, but a pain for our
} } constantly re-used & recycled Core Facility poster.
} }
} } However I would still advise against going to lower than say
} } 1,000x750 for
} } an image whatever size it's going to be printed, if you think you
} } might
} } possibly use it again. Our Core poster has 40+ images going back 5
} } years,
} } and the 'master' hi-res images are simply lost to history. Probably
} } they
} } were deleted from my processors personal hard drive space when he
} } left, and
} } probably they weren't all ours, but from our collaborators/users.
} } So it is
} } an incredible pain to find that all our images on our main poster
} } ppt files
} } are about 250x300 max pixel size - with multiple backups at different
} } locations, it's often only the poster master ppt file itself that
} } survives.
} } If, at a later date, I want to increase the printed image size from
} } 2x1.5"
} } to say 6x4.5" the pixilation is very noticeable. Plus they look bad
} } in Core
} } PowerPoint and pdf presentations - and we are supposed to be the
} } kings of
} } imaging. You can get by, say by upscaling and re-adding text at
} } higher res -
} } but what a pain, more hours in Photoshop, and with typical day
} } rates of
} } about £400+. And the extra file size of the larger images in the
} } original
} } ppt poster file would have been insignificant on a modern PC.
} }
} } Keeping track of digital images for the next 30 years is just about
} } impossible, if I want an image from one of my old papers these days
} } I have
} } scan the printed copy. I guess I produce 10,000+ digital images a
} } year at
} } home and at work these days. Backup is one thing, finding a
} } particular image
} } again quickly years on is another matter.
} }
} } Regards
} }
} } Keith
} }
} }
} } ---------------------------------------------------------------------------
} } Dr Keith J. Morris,
} } Molecular Cytogenetics and Microscopy Core,
} } Laboratory 00/069 and 00/070,
} } The Wellcome Trust Centre for Human Genetics,
} } Roosevelt Drive,
} } Oxford OX3 7BN,
} } United Kingdom.
} }
} }
} } Telephone: +44 (0)1865 287568
} } Email: kjmorris-at-well.ox.ac.uk
} } Web-pages: http://www.well.ox.ac.uk/cytogenetics/
}
} ==============================Original
} Headers==============================
} 17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009
} 17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk
} [163.1.2.169])
} 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n3LBEktC015545
} 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009
} 06:14:47 -0500
} 17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207])
} 17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
} 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
} 17, 28 -- id 1LwDw6-0002ch-UF
} 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46
} +0100
} 17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
} 17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
} 17, 28 -- (Exim 4.69)
} 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
} 17, 28 -- id 1LwDw6-0002Oo-3F
} 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46
} +0100
} 17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk}
} 17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100
} 17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
} 17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
} 17, 28 -- MIME-Version: 1.0
} 17, 28 -- To: microscopy-at-microscopy.com
} 17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of
} micrographs for posters?
} 17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
} 17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
} 17, 28 -- X-Enigmail-Version: 0.95.7
} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1
} 17, 28 -- Content-Transfer-Encoding: 8bit
} 17, 28 -- X-Oxford-Username: phar0293
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
11, 24 -- From vladislav_speransky-at-nih.gov Tue Apr 21 16:17:32 2009
11, 24 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107])
11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LLHMgC024307
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 16:17:32 -0500
11, 24 -- X-IronPortListener: NIH_Relay
11, 24 -- X-SBRS: None
11, 24 -- X-IronPort-AV: E=Sophos;i="4.40,226,1238990400";
11, 24 -- d="scan'208";a="86558312"
11, 24 -- Received: from helix.nih.gov ([128.231.2.3])
11, 24 -- by nihrelayxway2.hub.nih.gov with ESMTP; 21 Apr 2009 17:17:17 -0400
11, 24 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185])
11, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3LLHGf4031525
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:17:16 -0400
11, 24 -- Message-Id: {6FC32721-A969-4A83-A9D1-27B1EA35F08F-at-nih.gov}
11, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
11, 24 -- To: Microscopy-at-microscopy.com
11, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 24 -- Subject: Fwd: [Microscopy] Re: Advice: Resolution of micrographs for posters?
11, 24 -- Date: Tue, 21 Apr 2009 17:17:15 -0400
11, 24 -- References: {D13763A4740B07428566B25D7E768DA69F1C1AB2-at-NIHMLBX02.nih.gov}
11, 24 -- X-Mailer: Apple Mail (2.930.3)
11, 24 -- Content-Transfer-Encoding: 8bit
11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3LLHMgC024307
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Tue, 21 Apr 2009 16:37:48 -0500
Subject: [Microscopy] Re: OptiQuip Model 1200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paula,

OptiQuip is an excellent company run by Mel Decker, who has some
recent medical issues.

I've asked Mel to call you. If you do not hear from him give me a
call. We work together.

Disclaimer: Ladd Research supplies commercial products for laboratories.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 07:18 PM 4/20/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
14, 27 -- From jd-at-laddresearch.com Tue Apr 21 16:37:48 2009
14, 27 -- Received: from cernan.electric.net (cernan.electric.net [72.35.23.19])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LLblsJ032472
14, 27 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 16:37:48 -0500
14, 27 -- Received: from 1LwNes-0007JC-T4 by cernan.electric.net with emc1-ok (Exim 4.69)
14, 27 -- (envelope-from {jd-at-laddresearch.com} )
14, 27 -- id 1LwNeu-0007Vr-Td; Tue, 21 Apr 2009 14:37:40 -0700
14, 27 -- Received: by emcmailer; Tue, 21 Apr 2009 14:37:40 -0700
14, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
14, 27 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256)
14, 27 -- (Exim 4.69)
14, 27 -- (envelope-from {jd-at-laddresearch.com} )
14, 27 -- id 1LwNes-0007JC-T4; Tue, 21 Apr 2009 14:37:40 -0700
14, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 27 -- Date: Tue, 21 Apr 2009 17:37:23 -0400
14, 27 -- To: vapatpxs-at-yahoo.com
14, 27 -- From: jd {jd-at-laddresearch.com}
14, 27 -- Subject: Re: [Microscopy] OptiQuip Model 1200
14, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
14, 27 -- In-Reply-To: {200904202318.n3KNIWSi023830-at-ns.microscopy.com}
14, 27 -- References: {200904202318.n3KNIWSi023830-at-ns.microscopy.com}
14, 27 -- Mime-Version: 1.0
14, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
14, 27 -- X-Outbound-IP: 216.204.198.170
14, 27 -- X-Env-From: jd-at-laddresearch.com
14, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
14, 27 -- Message-Id: {E1LwNeu-0007Vr-Td-at-cernan.electric.net}
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Tue, 21 Apr 2009 17:56:35 -0500
Subject: [Microscopy] Re: Fwd: Re: Advice: Resolution of micrographs for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A Matter Of Education

As Vlad, Ben and others have commented, the BEST program is not
always the one being used to make posters. In our central facility,
we produce many hundreds of posters a year. The vast majority are
produced using PowerPoint, or, as our graphics person calls it,
ProblemPoint. If done properly (a rare event), the results can be as
good as those obtained using InDesign. However, when problems are
encountered, it takes a long time to figure out why PP is not
producing posters that print properly. We only rarely have this
problem with InDesign.

We have been using InDesign (and previously PageMaker, Quark and
Illustrator) to produce posters over the years, but when researchers
bring in "pre-made" posters, it is nearly aways a PP job. When
problems are encountered (usually due to the Postscript printer
balking at the PP hackjob), the harried researchers want to know "the
best" program to use. We always recommend they purchase the Adobe
Design Suite CS4. More and more researchers are doing this and the
word is slowly getting around. It will take time and patience.

This summer we will be giving a series of training sessions on how to
produce high quality posters using the CS4 Design Suite (Photoshop,
Illustrator, InDesign and Acrobat). We'll see if this improves the
situation. I believe it will.

JB

} Just thought I'd let you know that your nice write up on the
} advantages of InDesign has been appreciated! That does sound like the
} right way to keep your posters and related stuff organized. One of
} these days...
}
} On the meetings I have been too, though, your InDesign-made would be
} in a small minority, so please don't be too harsh on Keith... By far
} the majority of those posters I've seen have been done in PowerPoint.
} From that perspective, doing your poster in PP today is hardly "odd",
} although it is of course not the optimal way.
}
} Now back to being serious and constructive - this does sound like
} something people who make posters should be more aware of. Perhaps we
} could ask those Adobe representatives ("evangelists") to some
} tutorials during microscopy meetings? (I am thinking about M&M
} meetings in particular, but there are of course others...)

--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
9, 20 -- From bozzola-at-siu.edu Tue Apr 21 17:56:35 2009
9, 20 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu [131.230.1.2])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LMuYir026462
9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:56:35 -0500
9, 20 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136])
9, 20 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id n3LMuWnP029640
9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:56:33 -0500 (CDT)
9, 20 -- Mime-Version: 1.0
9, 20 -- Message-Id: {a06240805c613fccf82b0-at-[131.230.177.136]}
9, 20 -- In-Reply-To: {200904212123.n3LLNThU027008-at-ns.microscopy.com}
9, 20 -- References: {200904212123.n3LLNThU027008-at-ns.microscopy.com}
9, 20 -- Date: Tue, 21 Apr 2009 17:56:31 -0500
9, 20 -- To: Microscopy-at-microscopy.com
9, 20 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
9, 20 -- Subject: Re: [Microscopy] Fwd: Re: Advice: Resolution of micrographs for
9, 20 -- posters?
9, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
9, 20 -- X-Spam-Score: 0.00%
9, 20 -- X-MASF: 0.00%
9, 20 -- X-Whitelist: 0.00%
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Wed, 22 Apr 2009 05:29:09 -0500
Subject: [Microscopy] Fwd: Re: Advice: Resolution of micrographs for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our core poster was originally created in Powerpoint [not by me I might
add]. I had a go at converting it to Indesign CS3 but after an hour or so I
lost the will to live. Not helped by Adobe's obtuse help [I'm sure Adobe is
a front for the Bill Gate's evil empire, created simply to make Microsoft
software look good]. Adobe's stuff is buggy as well on first release. With
our Core poster, we only have to change the odd staff photo mainly, as
predecessor's relocate to the Solyent Green factory. Plus of course all the
images are mostly stuck at 250x300 resolution anyway and our ppt file always
prints OK [so if it's not broke..]. Indesign is much easier if you use it to
create the poster from scratch.

Having spent the last year wrestling with *Flash CS3's incomprehensible help
[often not even giving info on the right program], it doesn't make Indesign
that attractive for very occasional use - if you want to see how easy to use
a DTP program can be, try Serif's PagePlus X3, it makes Publisher look pants
[well I suppose Publisher is pants]. Trouble is the learning curve on Adobe
software is so steep you really have to use it every day to become
comfortable, not once a year. Quark Express is no better in terms of
intuitive use either. I did get to grips with GoLive, then Adobe killed it
off after buying MacroMedia. I use Illustrator occasionally, but mostly it's
Photoshop, Flash, Acrobat Pro, Dreamweaver and PhotoShop [again & again]. I
know Photoshop so well now I have fooled myself into thinking it's easy to
use. Besides it's Mat Lab and Dragon Naturally Speaking 10 that on my to-do
list for this month - plus of course Microsoft's 'intuitive' new ribbon
interface [I have kept Office 2003 installed with Office 2007 - easy to do,
with a few mods].

That said Adobe on-line video help is very very useful [but it's only
available for the latest products]. Try:

http://www.adobe.com/designcenter/video_workshop/?id=vid0118
Adobe Video Workshop

Plus we have our local OUCS courses and there's Amazon for a selection of
books.

Keith

*Are you unique? Why not find out
http://uniqueness.well.ox.ac.uk/language_set/introduction.php

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov]
Sent: 21 April 2009 22:58
To: kjmorris-at-well.ox.ac.uk

Ben,

Just thought I'd let you know that your nice write up on the
advantages of InDesign has been appreciated! That does sound like the
right way to keep your posters and related stuff organized. One of
these days...

On the meetings I have been too, though, your InDesign-made would be
in a small minority, so please don't be too harsh on Keith... By far
the majority of those posters I've seen have been done in PowerPoint.
From that perspective, doing your poster in PP today is hardly "odd",
although it is of course not the optimal way.

Now back to being serious and constructive - this does sound like
something people who make posters should be more aware of. Perhaps we
could ask those Adobe representatives ("evangelists") to some
tutorials during microscopy meetings? (I am thinking about M&M
meetings in particular, but there are of course others...)

Vlad

________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.


} I'm sorry if this sounds harsh Keith, but it seems a little odd to me
} that you use PowerPoint, designed for on-screen presentations, to
} produce posters.
}
} It is under £120 for you to buy InDesign CS4 (OUCS shop- license and
} media), and it will save you time as you never have to re-make posters
} for different sizes, and the process itself is a lot faster. You can
} probably justify the cost in time savings after only one or two
} posters.
}
} I know it doesn't address Kristen's question, as she is required to
} use
} PowerPoint. For importing into PowerPoint (or any Office application),
} PNG is the best file type, as it is the native compression for
} office.
} I would tend to go for 300ppi, just because computers can cope with
} large files, but realistically 210ppi is indistinguishable. Don't be
} confused with printers' dpi (typically inkjet for this application),
} and
} pixels per inch. Inkjets can not print continuous tone, they have to
} place many dots to achieve the colour information contained in a
} single
} pixel.
}
}
}
} Now to preach on the alternative :)
}
} InDesign links to the original full-resolution version of all images,
} via the 'place' command, and you can work with thousands of images of
} very large file sizes without it affecting the speed or reliability of
} the programme (it is designed for typesetting whole books after all)-
} this is because it only works with a low-resolution preview of the
} original file. The InDesign files themselves are never over a few
} megabytes
}
} You can 'package' all the linked images for a file together in the
} same
} place (this duplicates all the linked files and places them together-
} useful if you have linked to originals in multiple folders spread
} around
} your computer).
}
} When you want to have the poster printed, you export as a PDF,
} down-sampling all the images to the desired resolution and compression
} in one simple step. The result is a very robust file with all fonts
} embedded and almost fool-proof for printing, that is never too large
} or
} too small for the job.
}
} If you want to edit an image in the poster, you don't have to find
} your
} original high resolution file, and make changes, down-sample and
} re-import, as you would have to do in PowerPoint. Instead, you just
} right-click, and select 'edit original' from the contextual menu,
} and it
} opens the orignal in Photoshop, and after making changes you close the
} document, and the preview in InDesign will automatically update.
}
} If you ever use vector files, PowerPoint is a nightmare; where as
} InDesign links directly to Illustrator files, and changes are very
} quick. And the PDFs created by InDesign will obviously contain perfect
} copies of the vector information, so even if you do lose all version
} of
} a diagram, except a low-resolution PDF of a A0 poster designed for
} printing A4 as hand-outs, you can open the PDF in Illustrator and copy
} the perfect diagram out of the file.
}
} These are all in addition to the much more sophisticated text tools
} (justified text with complete control over the balance between
} enlarged
} spaces between words or letters, and hyphenation; word length before
} hyphenation should occur), automatic alignment tools and the ability
} to
} instantly spread items at equal spacing, or a pre-set spacing...
}
} Kind regards,
}
} Ben
}
}
} --
} Imaging Technician
} MRC Anatomical Neuropharmacology Unit, Mansfield Road,
} Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867
} {http://mrcanu.pharm.ox.ac.uk/}
}
} kjmorris-at-well.ox.ac.uk wrote:
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } I agree that 100dpi is perfectly fine for poster printing of most
} } images
} } where detail is difficult to discern anyway, say fluorescence in
} } cells or
} } tissues. You do notice low resolution jaggies in the text and in
} } things we
} } are good at discerning, say a microscope, illustrator image or lab
} } view
} } [when we know what they should look like]. Blurred printing on
} } cheap paper
} } [the standard for most poster printing] means that the 600dpi of a
} } modern
} } printer is unlikely ever to be realised. So although we easily
} } spend £2,000+
} } in man hours producing a poster, we rarely spend more than £40
} } printing it
} } [and for most purposes this poster resolution is perfectly adequate
} } anyway].
} } Plus the inks used may be rubbish, our University printed posters
} } have all
} } faded in a month or two after being left up in a windowless lab
} } [obviously
} } not HP inks and HP photographic paper where the inks are guaranteed
} } fast for
} } 100 years]. Fine for posters you use once and bin, but a pain for our
} } constantly re-used & recycled Core Facility poster.
} }
} } However I would still advise against going to lower than say
} } 1,000x750 for
} } an image whatever size it's going to be printed, if you think you
} } might
} } possibly use it again. Our Core poster has 40+ images going back 5
} } years,
} } and the 'master' hi-res images are simply lost to history. Probably
} } they
} } were deleted from my processors personal hard drive space when he
} } left, and
} } probably they weren't all ours, but from our collaborators/users.
} } So it is
} } an incredible pain to find that all our images on our main poster
} } ppt files
} } are about 250x300 max pixel size - with multiple backups at different
} } locations, it's often only the poster master ppt file itself that
} } survives.
} } If, at a later date, I want to increase the printed image size from
} } 2x1.5"
} } to say 6x4.5" the pixilation is very noticeable. Plus they look bad
} } in Core
} } PowerPoint and pdf presentations - and we are supposed to be the
} } kings of
} } imaging. You can get by, say by upscaling and re-adding text at
} } higher res -
} } but what a pain, more hours in Photoshop, and with typical day
} } rates of
} } about £400+. And the extra file size of the larger images in the
} } original
} } ppt poster file would have been insignificant on a modern PC.
} }
} } Keeping track of digital images for the next 30 years is just about
} } impossible, if I want an image from one of my old papers these days
} } I have
} } scan the printed copy. I guess I produce 10,000+ digital images a
} } year at
} } home and at work these days. Backup is one thing, finding a
} } particular image
} } again quickly years on is another matter.
} }
} } Regards
} }
} } Keith
} }
} }
} }
---------------------------------------------------------------------------
} } Dr Keith J. Morris,
} } Molecular Cytogenetics and Microscopy Core,
} } Laboratory 00/069 and 00/070,
} } The Wellcome Trust Centre for Human Genetics,
} } Roosevelt Drive,
} } Oxford OX3 7BN,
} } United Kingdom.
} }
} }
} } Telephone: +44 (0)1865 287568
} } Email: kjmorris-at-well.ox.ac.uk
} } Web-pages: http://www.well.ox.ac.uk/cytogenetics/
}
} ==============================Original
} Headers==============================
} 17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009
} 17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk
} [163.1.2.169])
} 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n3LBEktC015545
} 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009
} 06:14:47 -0500
} 17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207])
} 17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
} 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
} 17, 28 -- id 1LwDw6-0002ch-UF
} 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46
} +0100
} 17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12])
} 17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
} 17, 28 -- (Exim 4.69)
} 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} )
} 17, 28 -- id 1LwDw6-0002Oo-3F
} 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46
} +0100
} 17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk}
} 17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100
} 17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
} 17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
} 17, 28 -- MIME-Version: 1.0
} 17, 28 -- To: microscopy-at-microscopy.com
} 17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of
} micrographs for posters?
} 17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
} 17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com}
} 17, 28 -- X-Enigmail-Version: 0.95.7
} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1
} 17, 28 -- Content-Transfer-Encoding: 8bit
} 17, 28 -- X-Oxford-Username: phar0293
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
11, 24 -- From vladislav_speransky-at-nih.gov Tue Apr 21 16:17:32 2009
11, 24 -- Received: from nihrelayxway2.hub.nih.gov
(nihrelayxway2.hub.nih.gov [128.231.90.107])
11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3LLHMgC024307
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 16:17:32
-0500
11, 24 -- X-IronPortListener: NIH_Relay
11, 24 -- X-SBRS: None
11, 24 -- X-IronPort-AV: E=Sophos;i="4.40,226,1238990400";
11, 24 -- d="scan'208";a="86558312"
11, 24 -- Received: from helix.nih.gov ([128.231.2.3])
11, 24 -- by nihrelayxway2.hub.nih.gov with ESMTP; 21 Apr 2009 17:17:17
-0400
11, 24 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov
[128.231.217.185])
11, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id
n3LLHGf4031525
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:17:16
-0400
11, 24 -- Message-Id: {6FC32721-A969-4A83-A9D1-27B1EA35F08F-at-nih.gov}
11, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
11, 24 -- To: Microscopy-at-microscopy.com
11, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed;
delsp=yes
11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 24 -- Subject: Fwd: [Microscopy] Re: Advice: Resolution of micrographs
for posters?
11, 24 -- Date: Tue, 21 Apr 2009 17:17:15 -0400
11, 24 -- References:
{D13763A4740B07428566B25D7E768DA69F1C1AB2-at-NIHMLBX02.nih.gov}
11, 24 -- X-Mailer: Apple Mail (2.930.3)
11, 24 -- Content-Transfer-Encoding: 8bit
11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3LLHMgC024307
==============================End of - Headers==============================



==============================Original Headers==============================
26, 23 -- From kjmorris-at-well.ox.ac.uk Wed Apr 22 05:29:09 2009
26, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
26, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MAT6Cr032403
26, 23 -- for {microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 05:29:08 -0500
26, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
26, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
26, 23 -- id 1LwZhQ-0007fd-AV
26, 23 -- for microscopy-at-microscopy.com; Wed, 22 Apr 2009 11:29:04 +0100
26, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
26, 23 -- To: {microscopy-at-microscopy.com}
26, 23 -- References: {200904212158.n3LLwGVX016336-at-ns.microscopy.com}
26, 23 -- Subject: RE: [Microscopy] Fwd: Re: Advice: Resolution of micrographs for posters?
26, 23 -- Date: Wed, 22 Apr 2009 11:29:04 +0100
26, 23 -- Message-ID: {FD2979C9F8BC47AC9477225BE88EF942-at-CytoWhizz}
26, 23 -- MIME-Version: 1.0
26, 23 -- Content-Type: text/plain;
26, 23 -- charset="iso-8859-1"
26, 23 -- X-Mailer: Microsoft Office Outlook 11
26, 23 -- In-Reply-To: {200904212158.n3LLwGVX016336-at-ns.microscopy.com}
26, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
26, 23 -- thread-index: AcnCzEyKk0r+QyFXTf+UWzwD1PhRiQAYb6fA
26, 23 -- Content-Transfer-Encoding: 8bit
26, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MAT6Cr032403
==============================End of - Headers==============================




From: LettJ-at-ent.wustl.edu
Date: Wed, 22 Apr 2009 17:42:14 -0500
Subject: [Microscopy] TEM: demagnetizers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We need to purchase a demagnetizer, to use mostly for forceps (handling
nickel grids, etc). Not having any experience with these, I'd like some
opinions. We'd like to purchase the less expensive one, but not if the
general opinion is that it's not effective.

Ted Pella's runs $85-90
(http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS'
costs about $255
(http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as
px#62083).

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
660 S. Euclid Ave., Campus Box 8115
St. Louis, MO 63110

Email: lettj-at-ent.wustl.edu
http://otocore.wustl.edu



==============================Original Headers==============================
8, 23 -- From LettJ-at-ent.wustl.edu Wed Apr 22 17:42:14 2009
8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MMgEZs012661
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 17:42:14 -0500
8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Wed, 22 Apr 2009 17:42:10 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: TEM: demagnetizers
8, 23 -- Date: Wed, 22 Apr 2009 17:42:10 -0500
8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C39CB45FD-at-EX04.wusm-pcf.wustl.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: TEM: demagnetizers
8, 23 -- Thread-Index: AcnDm5IKJP+9b4MVS7KEH1yCTDyj9g==
8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 22 Apr 2009 22:42:10.0669 (UTC) FILETIME=[92C7E1D0:01C9C39B]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MMgEZs012661
==============================End of - Headers==============================




From: a.d.mckinnon-at-abdn.ac.uk
Date: Thu, 23 Apr 2009 03:24:40 -0500
Subject: [Microscopy] TEM: demagnetizers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alternatively, depending on how well de-magnetizers work, you could buy anti-magnetic forceps. I've no experience of de-magnetizers, but our anti-mag, anti capillary forceps (we got ours from TAAB www.taab.co.uk ) have stood the test of time working extensively with nickel grids.


Alastair McKinnon
IMS Histology & EM Facility Manager
University of Aberdeen,
Institute of Medical Sciences
Foresterhill, Aberdeen, AB25 2ZD
01224 552923; www.abdn.ac.uk/ims/h-em/

-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: 22 April 2009 23:48
To: Mckinnon, Alastair D.

We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective.

Ted Pella's runs $85-90
(http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS'
costs about $255
(http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as
px#62083).

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110

Email: lettj-at-ent.wustl.edu
http://otocore.wustl.edu



==============================Original Headers==============================
8, 23 -- From LettJ-at-ent.wustl.edu Wed Apr 22 17:42:14 2009 8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MMgEZs012661
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 17:42:14 -0500
8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Wed, 22 Apr 2009 17:42:10 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: TEM: demagnetizers
8, 23 -- Date: Wed, 22 Apr 2009 17:42:10 -0500 8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C39CB45FD-at-EX04.wusm-pcf.wustl.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: TEM: demagnetizers 8, 23 -- Thread-Index: AcnDm5IKJP+9b4MVS7KEH1yCTDyj9g== 8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 23 -- To: {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 22 Apr 2009 22:42:10.0669 (UTC) FILETIME=[92C7E1D0:01C9C39B] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MMgEZs012661 ==============================End of - Headers==============================


The University of Aberdeen is a charity registered in Scotland, No SC013683.


==============================Original Headers==============================
20, 29 -- From a.d.mckinnon-at-abdn.ac.uk Thu Apr 23 03:24:40 2009
20, 29 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.8])
20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3N8OdCo011457
20, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 03:24:40 -0500
20, 29 -- Received: from ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83] helo=mail.abdn.ac.uk)
20, 29 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52)
20, 29 -- id 1LwuEY-0001Ls-Ql; Thu, 23 Apr 2009 09:24:38 +0100
20, 29 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.92]) by
20, 29 -- ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83]) with mapi; Thu, 23 Apr 2009
20, 29 -- 09:24:38 +0100
20, 29 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk}
20, 29 -- To: "'LettJ-at-ent.wustl.edu'" {LettJ-at-ent.wustl.edu}
20, 29 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
20, 29 -- Date: Thu, 23 Apr 2009 09:24:38 +0100
20, 29 -- Subject: RE: [Microscopy] TEM: demagnetizers
20, 29 -- Thread-Topic: [Microscopy] TEM: demagnetizers
20, 29 -- Thread-Index: AcnDnHRfRdrGbDz3Soa4Bx76Z6CFXwATuUUg
20, 29 -- Message-ID: {A3317CA649BC8C4998EED327D66A8D95B346300E5A-at-VMAILB.uoa.abdn.ac.uk}
20, 29 -- References: {200904222248.n3MMmEIl019527-at-ns.microscopy.com}
20, 29 -- In-Reply-To: {200904222248.n3MMmEIl019527-at-ns.microscopy.com}
20, 29 -- Accept-Language: en-US
20, 29 -- Content-Language: en-US
20, 29 -- X-MS-Has-Attach:
20, 29 -- X-MS-TNEF-Correlator:
20, 29 -- acceptlanguage: en-US
20, 29 -- Content-Type: text/plain; charset="us-ascii"
20, 29 -- MIME-Version: 1.0
20, 29 -- Content-Transfer-Encoding: 8bit
20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3N8OdCo011457
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 23 Apr 2009 06:03:29 -0500
Subject: [Microscopy] TEM: demagnetizers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jaci,
I've had several of an older version of the Ladd demagnetizer
http://www.laddresearch.com/General_Catalog/Chapter_7/Small_Equipment___Inst
ruments/Miscellaneous_Small_Equipment/Demagnetizer/demagnetizer.html
which looks very much like the Pella. I've been quite happy over the past
28 years. Oh, I had several because I had several field engineers at one
point. I'm still using the first one I bought.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Wednesday, April 22, 2009 6:46 PM
To: kenconverse-at-qualityimages.biz

We need to purchase a demagnetizer, to use mostly for forceps (handling
nickel grids, etc). Not having any experience with these, I'd like some
opinions. We'd like to purchase the less expensive one, but not if the
general opinion is that it's not effective.

Ted Pella's runs $85-90
(http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS'
costs about $255
(http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as
px#62083).

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
660 S. Euclid Ave., Campus Box 8115
St. Louis, MO 63110

Email: lettj-at-ent.wustl.edu
http://otocore.wustl.edu



==============================Original Headers==============================
8, 23 -- From LettJ-at-ent.wustl.edu Wed Apr 22 17:42:14 2009
8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu
[128.252.17.171])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3MMgEZs012661
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 17:42:14
-0500
8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by
MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Wed, 22 Apr 2009 17:42:10 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: TEM: demagnetizers
8, 23 -- Date: Wed, 22 Apr 2009 17:42:10 -0500
8, 23 -- Message-ID:
{1C8B3F4710BBDD4CA24CB5269F2B4C39CB45FD-at-EX04.wusm-pcf.wustl.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: TEM: demagnetizers
8, 23 -- Thread-Index: AcnDm5IKJP+9b4MVS7KEH1yCTDyj9g==
8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 22 Apr 2009 22:42:10.0669 (UTC)
FILETIME=[92C7E1D0:01C9C39B]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3MMgEZs012661
==============================End of - Headers==============================




==============================Original Headers==============================
20, 25 -- From kenconverse-at-qualityimages.biz Thu Apr 23 06:03:28 2009
20, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3NB3Rcw031985
20, 25 -- for {microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 06:03:28 -0500
20, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
20, 25 -- with ESMTP
20, 25 -- id {20090423110325497.ELYC23317-at-cdptpa-omta01.mail.rr.com} ;
20, 25 -- Thu, 23 Apr 2009 11:03:25 +0000
20, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
20, 25 -- To: {LettJ-at-ent.wustl.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
20, 25 -- Subject: RE: [Microscopy] TEM: demagnetizers
20, 25 -- Date: Thu, 23 Apr 2009 07:03:19 -0400
20, 25 -- Message-ID: {DEBFD256016946A7B1B7AADDBF2D72DB-at-Ken}
20, 25 -- MIME-Version: 1.0
20, 25 -- Content-Type: text/plain;
20, 25 -- charset="us-ascii"
20, 25 -- X-Priority: 3 (Normal)
20, 25 -- X-MSMail-Priority: Normal
20, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
20, 25 -- Importance: Normal
20, 25 -- Thread-Index: AcnDnB8MNoseCbbQQ36yrwK6ZRbRgwAA4EMg
20, 25 -- In-Reply-To: {200904222246.n3MMk2JY016710-at-ns.microscopy.com}
20, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 25 -- Content-Transfer-Encoding: 8bit
20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3NB3Rcw031985
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 23 Apr 2009 06:09:31 -0500
Subject: [Microscopy] TEM: demagnetizers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We have the unit from Pella. This works very effectively on standard
forceps (or other tools) and allows one to either magnetize or
demagnetize by choice of which direction the forceps pass into the loop;
for some purposes having tools be magnetic is an advantage....

Non-magnetic forceps are nice also, but I believe that I read that the
steel is not as hard as the standard grades of the same manufacturer -
one of the suppliers (EMS?) had a lot of info at their website on the
styles/materials of the Dumont line.

Dale

a.d.mckinnon-at-abdn.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Alternatively, depending on how well de-magnetizers work, you could buy anti-magnetic forceps. I've no experience of de-magnetizers, but our anti-mag, anti capillary forceps (we got ours from TAAB www.taab.co.uk ) have stood the test of time working extensively with nickel grids.
}
}
} Alastair McKinnon
} IMS Histology & EM Facility Manager
} University of Aberdeen,
} Institute of Medical Sciences
} Foresterhill, Aberdeen, AB25 2ZD
} 01224 552923; www.abdn.ac.uk/ims/h-em/
}
} -----Original Message-----
} X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
} Sent: 22 April 2009 23:48
} To: Mckinnon, Alastair D.
} Subject: [Microscopy] TEM: demagnetizers
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective.
}
} Ted Pella's runs $85-90
} (http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS'
} costs about $255
} (http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as
} px#62083).
}
} Thank you,
}
} Jaci
}
} Jaclynn Lett
} Senior Research Technician, EM Facility
} Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
}
} Email: lettj-at-ent.wustl.edu
} http://otocore.wustl.edu
}
}
}
} ==============================Original Headers==============================
} 8, 23 -- From LettJ-at-ent.wustl.edu Wed Apr 22 17:42:14 2009 8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171])
} 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MMgEZs012661
} 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 17:42:14 -0500
} 8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 23 -- Wed, 22 Apr 2009 17:42:10 -0500
} 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain;
} 8, 23 -- charset="us-ascii"
} 8, 23 -- Subject: TEM: demagnetizers
} 8, 23 -- Date: Wed, 22 Apr 2009 17:42:10 -0500 8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C39CB45FD-at-EX04.wusm-pcf.wustl.edu}
} 8, 23 -- X-MS-Has-Attach:
} 8, 23 -- X-MS-TNEF-Correlator:
} 8, 23 -- Thread-Topic: TEM: demagnetizers 8, 23 -- Thread-Index: AcnDm5IKJP+9b4MVS7KEH1yCTDyj9g== 8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 23 -- To: {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 22 Apr 2009 22:42:10.0669 (UTC) FILETIME=[92C7E1D0:01C9C39B] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MMgEZs012661 ==============================End of - Headers==============================
}
}
} The University of Aberdeen is a charity registered in Scotland, No SC013683.
}
}
} ==============================Original Headers==============================
} 20, 29 -- From a.d.mckinnon-at-abdn.ac.uk Thu Apr 23 03:24:40 2009
} 20, 29 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.8])
} 20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3N8OdCo011457
} 20, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 03:24:40 -0500
} 20, 29 -- Received: from ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83] helo=mail.abdn.ac.uk)
} 20, 29 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52)
} 20, 29 -- id 1LwuEY-0001Ls-Ql; Thu, 23 Apr 2009 09:24:38 +0100
} 20, 29 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.92]) by
} 20, 29 -- ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83]) with mapi; Thu, 23 Apr 2009
} 20, 29 -- 09:24:38 +0100
} 20, 29 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk}
} 20, 29 -- To: "'LettJ-at-ent.wustl.edu'" {LettJ-at-ent.wustl.edu}
} 20, 29 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
} 20, 29 -- Date: Thu, 23 Apr 2009 09:24:38 +0100
} 20, 29 -- Subject: RE: [Microscopy] TEM: demagnetizers
} 20, 29 -- Thread-Topic: [Microscopy] TEM: demagnetizers
} 20, 29 -- Thread-Index: AcnDnHRfRdrGbDz3Soa4Bx76Z6CFXwATuUUg
} 20, 29 -- Message-ID: {A3317CA649BC8C4998EED327D66A8D95B346300E5A-at-VMAILB.uoa.abdn.ac.uk}
} 20, 29 -- References: {200904222248.n3MMmEIl019527-at-ns.microscopy.com}
} 20, 29 -- In-Reply-To: {200904222248.n3MMmEIl019527-at-ns.microscopy.com}
} 20, 29 -- Accept-Language: en-US
} 20, 29 -- Content-Language: en-US
} 20, 29 -- X-MS-Has-Attach:
} 20, 29 -- X-MS-TNEF-Correlator:
} 20, 29 -- acceptlanguage: en-US
} 20, 29 -- Content-Type: text/plain; charset="us-ascii"
} 20, 29 -- MIME-Version: 1.0
} 20, 29 -- Content-Transfer-Encoding: 8bit
} 20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3N8OdCo011457
} ==============================End of - Headers==============================

==============================Original Headers==============================
5, 20 -- From dac-at-research.umass.edu Thu Apr 23 06:09:31 2009
5, 20 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3NB9TCe004213
5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 06:09:30 -0500
5, 20 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged))
5, 20 -- (authenticated bits=0)
5, 20 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n3NB9RX9028536
5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 07:09:27 -0400
5, 20 -- Message-ID: {49F04C68.1040203-at-research.umass.edu}
5, 20 -- Date: Thu, 23 Apr 2009 07:09:28 -0400
5, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
5, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16
5, 20 -- MIME-Version: 1.0
5, 20 -- To: Microscopy-at-microscopy.com
5, 20 -- Subject: Re: [Microscopy] RE: TEM: demagnetizers
5, 20 -- References: {200904230829.n3N8Tpi7019605-at-ns.microscopy.com}
5, 20 -- In-Reply-To: {200904230829.n3N8Tpi7019605-at-ns.microscopy.com}
5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 23 Apr 2009 11:52:07 -0500
Subject: [Microscopy] Re: TEM: demagnetizers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try Radio Shack or Fry's. These are mag tape
degaussing units. Usually around $25.

Here's one from Digikey for $6:

http://search.digikey.com/scripts/DkSearch/dksus.dll?Detail&name=431-1056-ND

gary g.


At 03:44 PM 4/22/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Thu Apr 23 11:52:07 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3NGq6Z5009457
10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 11:52:06 -0500
10, 20 -- Message-Id: {200904231652.n3NGq6Z5009457-at-ns.microscopy.com}
10, 20 -- Received: (qmail 19109 invoked from network); 23 Apr 2009 09:47:34 -0700
10, 20 -- Received: by simscan 1.1.0 ppid: 19104, pid: 19105, t: 0.1069s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 23 Apr 2009 09:47:34 -0700
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Thu, 23 Apr 2009 09:51:57 -0700
10, 20 -- To: LettJ-at-ent.wustl.edu
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] TEM: demagnetizers
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200904222244.n3MMiPYh015111-at-ns.microscopy.com}
10, 20 -- References: {200904222244.n3MMiPYh015111-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: leolukman-at-wisc.edu
Date: Thu, 23 Apr 2009 13:21:11 -0500
Subject: [Microscopy] sectioning p4vp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

I'm a graduate student at Materials Science Program. I tried to section a poly(4-vinylpyridine) (P4VP) embedded in epon with a diamond knife and water on the boat. However, it seemed that the water ate away my sample and the knife only cut the epon. If i looked at the sample face after trying to section, i could see that the sample face had become rough, which i assumed because the water somehow dissolving the sample (In large amount, P4VP is insoluble in water). Would you please suggest me how to resolve this problem? Thank you very much.

Sincerely,
Melvina Leolukman

==============================Original Headers==============================
3, 33 -- From leolukman-at-wisc.edu Thu Apr 23 13:21:09 2009
3, 33 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210])
3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3NIL85O027207
3, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 13:21:08 -0500
3, 33 -- MIME-version: 1.0
3, 33 -- Content-transfer-encoding: 7BIT
3, 33 -- Content-disposition: inline
3, 33 -- Content-type: text/plain; charset=us-ascii
3, 33 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by
3, 33 -- smtpauth1.wiscmail.wisc.edu
3, 33 -- (Sun Java(tm) System Messaging Server 7.0-5.01 32bit (built Feb 19 2009))
3, 33 -- id {0KIK00E02GB8KN00-at-smtpauth1.wiscmail.wisc.edu} for
3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:08 -0500 (CDT)
3, 33 -- Received: from wiscmail.wisc.edu (store1.doit.wisc.edu [144.92.8.164])
3, 33 -- by smtpauth1.wiscmail.wisc.edu
3, 33 -- (Sun Java(tm) System Messaging Server 7.0-5.01 32bit (built Feb 19 2009))
3, 33 -- with ESMTP id {0KIK009W9GB76120-at-smtpauth1.wiscmail.wisc.edu} for
3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:07 -0500 (CDT)
3, 33 -- Received: from [144.92.8.222] (Forwarded-For: 128.104.185.23, [144.92.197.247])
3, 33 -- by store1.doit.wisc.edu (mshttpd); Thu, 23 Apr 2009 13:21:07 -0500
3, 33 -- Date: Thu, 23 Apr 2009 13:21:07 -0500
3, 33 -- From: MELVINA LEOLUKMAN {leolukman-at-wisc.edu}
3, 33 -- Subject: sectioning p4vp
3, 33 -- To: Microscopy-at-microscopy.com
3, 33 -- Message-id: {e51c89d31f6ec.49f06b43-at-wiscmail.wisc.edu}
3, 33 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-8.04 (built Feb 28 2007)
3, 33 -- Content-language: en
3, 33 -- X-Accept-Language: en
3, 33 -- Priority: normal
3, 33 -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.164
3, 33 -- X-Spam-PmxInfo: Server=avs-10, Version=5.5.1.360522,
3, 33 -- Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.4.23.180432,
3, 33 -- SenderIP=144.92.8.164
==============================End of - Headers==============================




From: peter.heimann-at-uni-bielefeld.de
Date: Fri, 24 Apr 2009 05:52:55 -0500
Subject: [Microscopy] ? surplus / unused / used Ultracut E or Ultracut S in Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,
my (genial) service-technician wants to buy a Reichert Ultracut "E" or "S".
If anybody wants to sell a surplus / unused / used Ultracut E or
Ultracut S in Europe, please contact Mr Volker Busse directly by mail
Techno-Med-at-t-online.de

greetings,
peter heimann

--
====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107
33501 Bielefeld, Germany www.uni-bielefeld.de/biologie/cellbio



==============================Original Headers==============================
5, 31 -- From peter.heimann-at-uni-bielefeld.de Fri Apr 24 05:52:55 2009
5, 31 -- Received: from mux2-unibi-smtp.hrz.uni-bielefeld.de (mux2-unibi-smtp.hrz.uni-bielefeld.de [129.70.204.73])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OAqsQk015718
5, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 05:52:55 -0500
5, 31 -- MIME-version: 1.0
5, 31 -- Content-transfer-encoding: 7BIT
5, 31 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
5, 31 -- Received: from pmxchannel-daemon.mux2-unibi-smtp.hrz.uni-bielefeld.de by
5, 31 -- mux2-unibi-smtp.hrz.uni-bielefeld.de
5, 31 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
5, 31 -- id {0KIL00700Q85WX00-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} for
5, 31 -- Microscopy-at-microscopy.com; Fri, 24 Apr 2009 12:52:53 +0200 (CEST)
5, 31 -- Received: from [129.70.82.133] ([129.70.82.133])
5, 31 -- by mux2-unibi-smtp.hrz.uni-bielefeld.de
5, 31 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
5, 31 -- with ESMTPPSA id {0KIL00LA3Q84UZE0-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} ; Fri,
5, 31 -- 24 Apr 2009 12:52:53 +0200 (CEST)
5, 31 -- Date: Fri, 24 Apr 2009 12:52:51 +0200
5, 31 -- From: PETER HEIMANN {peter.heimann-at-uni-bielefeld.de}
5, 31 -- Subject: ? surplus / unused / used Ultracut E or Ultracut S in Europe
5, 31 -- To: Microscopy-at-microscopy.com, Volker Busse {Techno-Med-at-t-online.de}
5, 31 -- Reply-to: peter.heimann-at-uni-bielefeld.de
5, 31 -- Message-id:
5, 31 -- {23690_1240570373_ZZg0t66ooU1FH.00_49F19A03.2070708-at-UNI-BIELEFELD.DE}
5, 31 -- Organization: Uni Bielefeld
5, 31 -- X-Accept-Language: en-us, en
5, 31 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de
5, 31 -- X-PMX-Version: 5.5.1.360522, Antispam-Engine: 2.6.1.350677,
5, 31 -- Antispam-Data: 2009.4.24.104041, pmx7
5, 31 -- X-Connecting-IP: 129.70.82.133
5, 31 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317)
==============================End of - Headers==============================




From: aforlenza-at-nikon.net
Date: Fri, 24 Apr 2009 08:20:02 -0500
Subject: [Microscopy] viaWWW: 2009 Nikon International Small World Competition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both aforlenza-at-nikon.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: aforlenza-at-nikon.net
Name: Allison Forlenza

Organization: Nikon Instruments Inc.

Title-Subject: [Filtered] 2009 Nikon International Small World Competition

Question: Have a beautiful photomicrograph you would like to share
with the world? Nikon invites you to showcase your photomicrographs
in the 2009 Nikon International Small World Competition. Every year,
Nikon receives beautiful images for submission and would love to view
yours this year. Last year the winning photographers and their
images were viewed by over 39 million people! The subject matter is
unrestricted and any light microscopy technique is acceptable. This
is the perfect format for the scientific community to share their
science and creative eye with the world. The deadline of April 30,
2009 is quickly approaching, so hurry to www.nikonsmallworld.com for
entry and contest rules. Receive a free Small World calendar just
for entering!

Login Host: 66.11.134.11
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:01 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODK0sV003338
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:01 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c6176cef4c5f-at-[206.69.208.22]}
6, 11 -- Date: Fri, 24 Apr 2009 08:19:59 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: aforlenza-at-nikon.net (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: 2009 Nikon International Small World Competition
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hagglundk1-at-nku.edu
Date: Fri, 24 Apr 2009 08:20:25 -0500
Subject: [Microscopy] viaWWW: Maltese Cross in polarized light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hagglundk1-at-nku.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hagglundk1-at-nku.edu
Name: Karl Hagglund

Title-Subject: [Filtered] Maltese Cross in polarized light microscopy

Question: I have a customer who has asked about the formation of the
maltese cross interference pattern under crossed polarized light. A
common example of this can be seen in a variety of starches viewed
under crossed polars.

Can someone provide a concise explanation or a good reference that
explains why we see the maltese cross?

Thanks in advance,

Karl

Login Host: 199.64.0.252
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:25 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODKOSr003559
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:25 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6176d07521b-at-[206.69.208.22]}
8, 11 -- Date: Fri, 24 Apr 2009 08:20:23 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: hagglundk1-at-nku.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Maltese Cross in polarized light microscopy
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Lubomir.Kovacik-at-lf1.cuni.cz
Date: Fri, 24 Apr 2009 08:20:48 -0500
Subject: [Microscopy] viaWWW: looking for a Zeiss 902 trackball

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Lubomir.Kovacik-at-lf1.cuni.cz as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Lubomir.Kovacik-at-lf1.cuni.cz
Name: Lubomir Kovacik

Organization: Institute of Cell biology and Pathology, Charles Uni, Prague

Title-Subject: [Filtered] looking for a Zeiss 902 trackball

Question: Dear Fellow Microscopists,

we have a Zeiss TEM 902 that we would like to keep in operation for
students. However, we are missing a functional trackball, which is
also not available from the service. If you come across one in your
lab's cellar, please do not throw it away but let us know!

With best regards,

Lubomir Kovacik

Institute of Cellular Biology and Pathology
1st Faculty of Medicine, Charles University
Prague
Czech Republic


Login Host: 195.113.48.5
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:48 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODKlo9003955
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:48 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240802c6176d2057de-at-[206.69.208.22]}
11, 11 -- Date: Fri, 24 Apr 2009 08:20:46 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: Lubomir.Kovacik-at-lf1.cuni.cz (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: looking for a Zeiss 902 trackball
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 24 Apr 2009 09:10:36 -0500
Subject: [Microscopy] Re: viaWWW: Maltese Cross in polarized light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Karl,

Starch molecules in the starch grains are laid down in concentric
layers. This arrangement gives rise to form birefringence. The same
would be true form molecules in a radial arrangement. Both arrangements
will rotate the incident polarized light beam so that the light will
pass the second polarizer (analyzer) in these quadrants, giving the
"maltese cross". Additionally, by using an appropriate "compensator
plate", one can observe "addition and subtraction colors" in the
orthogonal regions of brightness and distinguish the circumferential
from the radial arrangement.

There is a wealth of detailed information at:
http://micro.magnet.fsu.edu/primer/techniques/polarized/polarizedhome.html

We have a book in our library with an excellent article by H. Stanley
Bennett on polarized light microscopy. I think this is the book:
McClung's handbook of microscopial technique for workers in animal and
plant tissues, by thirty-five authors. Edited by Ruth McClung Jones.
New York, Hafner Pub. Co. [1964, c1950]
xix, 790 p. illus., diagrs. 24 cm.

If this isn't the correct reference I will track it down for you. The
Molecular Expressions information (link above) should have all you need.

Hope this helps.

Dale

hagglundk1-at-nku.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both hagglundk1-at-nku.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: hagglundk1-at-nku.edu
} Name: Karl Hagglund
}
} Title-Subject: [Filtered] Maltese Cross in polarized light microscopy
}
} Question: I have a customer who has asked about the formation of the
} maltese cross interference pattern under crossed polarized light. A
} common example of this can be seen in a variety of starches viewed
} under crossed polars.
}
} Can someone provide a concise explanation or a good reference that
} explains why we see the maltese cross?
}
} Thanks in advance,
}
} Karl
}
} Login Host: 199.64.0.252
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:25 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODKOSr003559
} 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:25 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c6176d07521b-at-[206.69.208.22]}
} 8, 11 -- Date: Fri, 24 Apr 2009 08:20:23 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: hagglundk1-at-nku.edu (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Maltese Cross in polarized light microscopy
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================

==============================Original Headers==============================
8, 22 -- From dac-at-research.umass.edu Fri Apr 24 09:10:35 2009
8, 22 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OEAZ2Q014700
8, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 09:10:35 -0500
8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
8, 22 -- (authenticated bits=0)
8, 22 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n3OEAXJ9002828
8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
8, 22 -- Fri, 24 Apr 2009 10:10:34 -0400
8, 22 -- Message-ID: {49F1C85A.509-at-research.umass.edu}
8, 22 -- Date: Fri, 24 Apr 2009 10:10:34 -0400
8, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
8, 22 -- Reply-To: dac-at-research.umass.edu
8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090303 SeaMonkey/1.1.15
8, 22 -- MIME-Version: 1.0
8, 22 -- To: hagglundk1-at-nku.edu, Microscopy Listserver {Microscopy-at-microscopy.com}
8, 22 -- Subject: Re: [Microscopy] viaWWW: Maltese Cross in polarized light microscopy
8, 22 -- References: {200904241334.n3ODYJlU027734-at-ns.microscopy.com}
8, 22 -- In-Reply-To: {200904241334.n3ODYJlU027734-at-ns.microscopy.com}
8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 24 Apr 2009 09:16:56 -0500
Subject: [Microscopy] Re: viaWWW: Maltese Cross in polarized light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Karl,
The Maltese cross, is in my opinion, is one of coolest things you can find
by PLM. Starch grains show a wide range of these effects and make finding
raw starches so much fun. Try it with your 1st order red for cooler
colors.

The effect is due to spherical orientation of crystalline material from a
central point. These molecules or microcrystals show extinction positions
at the 90, 180, 270 and 360 positions as well as compensation like all
birefringent material. Since your polars are at 90 degrees you get a black
extinction cross becase there are always some crystals at extriction. The
different colors with your 1st order red plate is due because in one
direction the crystals add and in the other they subtract. With low order
white colors the 1st order red produces two quadrants, as defined by the
cross, blue and the other yellow.

I use to find spherlites in degraded polyester, but some organic materials,
DDT for one, form colorful spherlites.

Way too much fun with PLM!

Frank



hagglundk1-at-nku.ed
u
To
04/24/2009 09:40 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] viaWWW: Maltese Cross
hagglundk1-at-nku.ed in polarized light microscopy
u












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both hagglundk1-at-nku.edu as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: hagglundk1-at-nku.edu
Name: Karl Hagglund

Title-Subject: [Filtered] Maltese Cross in polarized light microscopy

Question: I have a customer who has asked about the formation of the
maltese cross interference pattern under crossed polarized light. A
common example of this can be seen in a variety of starches viewed
under crossed polars.

Can someone provide a concise explanation or a good reference that
explains why we see the maltese cross?

Thanks in advance,

Karl

Login Host: 199.64.0.252
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:25 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n3ODKOSr003559
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009
08:20:25 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6176d07521b-at-[206.69.208.22]}
8, 11 -- Date: Fri, 24 Apr 2009 08:20:23 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: hagglundk1-at-nku.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Maltese Cross in polarized light microscopy
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
28, 22 -- From frank_karl-at-lincolnelectric.com Fri Apr 24 09:16:56 2009
28, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OEGti4021198
28, 22 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 09:16:55 -0500
28, 22 -- In-Reply-To: {200904241340.n3ODeNc3008559-at-ns.microscopy.com}
28, 22 -- Subject: Re: [Microscopy] viaWWW: Maltese Cross in polarized light microscopy
28, 22 -- To: hagglundk1-at-nku.edu, Microscopy-at-microscopy.com
28, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
28, 22 -- Message-ID: {OF043FD432.08590E61-ON852575A2.004B3B88-852575A2.004E6550-at-lincolnelectric.com}
28, 22 -- Date: Fri, 24 Apr 2009 10:16:26 -0400
28, 22 -- From: Frank_Karl-at-lincolnelectric.com
28, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM,
28, 22 -- CD-MIME complete at 04/24/2009 10:16:27 AM,
28, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM,
28, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM,
28, 22 -- Serialize complete at 04/24/2009 10:16:27 AM
28, 22 -- MIME-Version: 1.0
28, 22 -- Content-Type: text/plain;
28, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Fri, 24 Apr 2009 10:04:46 -0500
Subject: [Microscopy] Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Every once and a while we run into problems with sections falling off
the grids. I know this has been rehashed more than once here, but its
hard to find the answers in the archives, so I am asking for the
consolidated, final analysis of the problem and solutions.

I suspect it could be students using old, oxidized grids, but I'm not
sure enough to just give them that one answer.

I have check on 'grid glues' and searched around, but knew the experts
and experienced would be here.

So, what is your strategy. Never had the problem? Clean the grids
with solvents or acid, which ones? Grid glue or other solutions?

Lay it on me baby.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009
11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500
11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT)
11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236])
11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT)
11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700
11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24
11, 42 -- Apr 2009 07:47:04 -0700
11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT)
11, 42 -- X-Virus-Scanned: amavisd-new at
11, 42 -- X-Spam-Flag: NO
11, 42 -- X-Spam-Score: -2.498
11, 42 -- X-Spam-Level:
11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000,
11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ;
11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146])
11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu}
11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
11, 42 -- To: microscopy-at-microscopy.com
11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 42 -- Content-Transfer-Encoding: 7bit
11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 42 -- Subject: Sections falling off grids
11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700
11, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Fri, 24 Apr 2009 10:25:47 -0500
Subject: [Microscopy] Re: Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning/good afternoon,
Hi all,

Jonathan, not having had such problems in the long years I'm doing my job here I wonder about the } quality { and kind/type of resin sections you usually are dealing with.

Please could you provide information about wether this is a problem without a relationship to any or this is rellated to a specific resin type you use/ which is used.

Also it would be of interest wether you are talking about "falling off" sections mounted on "naked" copper (standard) or other specific grids (like nickel) or such one prefilmed with/by formvar/butvar/collodion film....

Thanking you and
best wishes and regards

Wolfgang MUSS
Salzburg, Austria



================================================================================================================
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
=================================================================================================================

} -----Ursprüngliche Nachricht-----
} Von: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
} Gesendet: Freitag, 24. April 2009 17:11
} An: Muß Wolfgang
} Betreff: [Microscopy] Sections falling off grids
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi:
}
} Every once and a while we run into problems with sections falling off the grids. I know this has been rehashed more than once here, but its hard to find the answers in the archives, so I am asking for the consolidated, final analysis of the problem and solutions.
}
} I suspect it could be students using old, oxidized grids, but I'm not sure enough to just give them that one answer.
}
} I have check on 'grid glues' and searched around, but knew the experts and experienced would be here.
}
} So, what is your strategy. Never had the problem? Clean the grids with solvents or acid, which ones?
} Grid glue or other solutions?
}
} Lay it on me baby.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original
} Headers==============================
} 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009
} 11, 42 -- Received: from mailin.deltacollege.edu
} (mailin.deltacollege.edu [207.62.178.150])
} 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id n3OF4QiE012517
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr
} 2009 10:04:44 -0500
} 11, 42 -- Received: from mailin.deltacollege.edu
} (localhost.localdomain [127.0.0.1])
} 11, 42 -- by localhost (Email Security Appliance) with



From: dac-at-research.umass.edu
Date: Fri, 24 Apr 2009 10:49:01 -0500
Subject: [Microscopy] Re: Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi.

I have had this trouble in the past but not for a long time since I
bagan flaming the grids. I saw this in the Bozzola and Russell book.
Works better for standard grids; be very careful with thin bar grids.
Use an alcohol burner with a small flame (~1cm max). Pick up a grid with
forceps and sweep briefly through the tip of the flame. It is better to
do too little than too much since it can be repeated until the desired
effect is acheived; after a bit you get it right almost always in one or
2 passes. What you are looking for is a "scorched" look, some
interference colors in the red and blue range (which must be thicknesses
of surface modification (oxidation?). These grids wet beautifully and
sections cling tenaciously. For the record, I always pick up sections on
the shiny side; I know there are 2 teams on this topic :-) My logic is
that it is like kitchen plastic film that clings better to smooth
surfaces....

I have had wettability issues with gold and gided grids that can't be
flamed. For these I treat 15 sec in the Harrick Plasma cleaner and they
wet beautifully and sections adhere well. This probably works for the
copper grids as well but I haven't tried.

Cheers!

Dale

jkrupp-at-deltacollege.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi:
}
} Every once and a while we run into problems with sections falling off
} the grids. I know this has been rehashed more than once here, but its
} hard to find the answers in the archives, so I am asking for the
} consolidated, final analysis of the problem and solutions.
}
} I suspect it could be students using old, oxidized grids, but I'm not
} sure enough to just give them that one answer.
}
} I have check on 'grid glues' and searched around, but knew the experts
} and experienced would be here.
}
} So, what is your strategy. Never had the problem? Clean the grids
} with solvents or acid, which ones? Grid glue or other solutions?
}
} Lay it on me baby.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original Headers==============================
} 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009
} 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
} 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500
} 11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
} 11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT)
} 11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236])
} 11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT)
} 11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
} 11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700
} 11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
} 11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
} 11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24
} 11, 42 -- Apr 2009 07:47:04 -0700
} 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT)
} 11, 42 -- X-Virus-Scanned: amavisd-new at
} 11, 42 -- X-Spam-Flag: NO
} 11, 42 -- X-Spam-Score: -2.498
} 11, 42 -- X-Spam-Level:
} 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000,
} 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
} 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
} 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ;
} 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
} 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146])
} 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
} 11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu}
} 11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
} 11, 42 -- To: microscopy-at-microscopy.com
} 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 11, 42 -- Content-Transfer-Encoding: 7bit
} 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 11, 42 -- Subject: Sections falling off grids
} 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700
} 11, 42 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of - Headers==============================

==============================Original Headers==============================
6, 22 -- From dac-at-research.umass.edu Fri Apr 24 10:49:01 2009
6, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OFn1fb009775
6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:49:01 -0500
6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
6, 22 -- (authenticated bits=0)
6, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n3OFmxxn015910
6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:49:00 -0400
6, 22 -- Message-ID: {49F1DF7C.7010908-at-research.umass.edu}
6, 22 -- Date: Fri, 24 Apr 2009 11:49:16 -0400
6, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
6, 22 -- Reply-To: dac-at-research.umass.edu
6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090303 SeaMonkey/1.1.15
6, 22 -- MIME-Version: 1.0
6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 22 -- Subject: Re: [Microscopy] Sections falling off grids
6, 22 -- References: {200904241513.n3OFDi56020840-at-ns.microscopy.com}
6, 22 -- In-Reply-To: {200904241513.n3OFDi56020840-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 24 Apr 2009 10:56:02 -0500
Subject: [Microscopy] Re: Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,
I routinely use a 'grid glue' as I like to immunolabel my sections
immersed in the immunoreagents as it makes the whole labelling process
easier and enhances the labelling, with the antibody/antibodies having
access to epitopes on both section surfaces. This latter means there is a
tendency to lose sections when transferring grids from one solution to the
next.
Anyway my trick is to just briefly immerse the grids in c.5ml of
chloroform in which about 4-5" of sellotape has been dissolved (remove the
tape itself once the glue has dissolved off it - just shake for a few
moments).
This seems to work for me and hope it does for you!
Cheers,
Jules

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

--On 24 April 2009 10:18 -0500 jkrupp-at-deltacollege.edu wrote:

}
}
}
} -------------------------------------------------------------------------
} --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------
} ---
}
} Hi:
}
} Every once and a while we run into problems with sections falling off
} the grids. I know this has been rehashed more than once here, but its
} hard to find the answers in the archives, so I am asking for the
} consolidated, final analysis of the problem and solutions.
}
} I suspect it could be students using old, oxidized grids, but I'm not
} sure enough to just give them that one answer.
}
} I have check on 'grid glues' and searched around, but knew the experts
} and experienced would be here.
}
} So, what is your strategy. Never had the problem? Clean the grids
} with solvents or acid, which ones? Grid glue or other solutions?
}
} Lay it on me baby.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu
}
}
}
}
} ==============================Original
} Headers============================== 11, 42 -- From
} jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009
} 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu
} [207.62.178.150]) 11, 42 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517 11, 42 -- for
} {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500 11, 42 --
} Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
} 11, 42 -- by localhost (Email Security Appliance) with SMTP id
} 366EB16B1BB_9F1CEF7B 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24
} Apr 2009 14:38:47 +0000 (GMT) 11, 42 -- Received: from sjdccd.cc.ca.us
} (unknown [207.62.178.236]) 11, 42 -- by mailin.deltacollege.edu (Sophos
} Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F 11, 42 -- for
} {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT) 11, 42
} -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 11, 42
} -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
} 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24
} Apr 2009 08:04:16 -0700 11, 42 -- Received: from zmail.deltacollege.edu
} ([207.62.178.179]) by 11, 42 -- sunspot.sjdccd.cc.ca.us
} (Netscape Messaging Server 4.15) with 11, 42 -- ESMTP id
} KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24 11, 42 --
} Apr 2009 07:47:04 -0700
} 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15
} -0700 (PDT) 11, 42 -- X-Virus-Scanned: amavisd-new at
} 11, 42 -- X-Spam-Flag: NO
} 11, 42 -- X-Spam-Score: -2.498
} 11, 42 -- X-Spam-Level:
} 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6
} tests=[AWL=0.000, 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
} 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
} 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1])
} (amavisd-new, port 10024) 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for
} {microscopy-at-microscopy.com} ; 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700
} (PDT)
} 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146])
} 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040
} 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14
} -0700 (PDT) 11, 42 -- Message-Id:
} {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu} 11, 42 -- From:
} Jon Krupp {jkrupp-at-deltacollege.edu}
} 11, 42 -- To: microscopy-at-microscopy.com
} 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes 11, 42 -- Content-Transfer-Encoding: 7bit
} 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
} 11, 42 -- Subject: Sections falling off grids
} 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700
} 11, 42 -- X-Mailer: Apple Mail (2.930.3)
} ==============================End of -
} Headers==============================





==============================Original Headers==============================
9, 24 -- From bafg3-at-sussex.ac.uk Fri Apr 24 10:56:01 2009
9, 24 -- Received: from karpinski.uscs.susx.ac.uk (karpinski.uscs.susx.ac.uk [139.184.14.85])
9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OFu1ak023976
9, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:56:01 -0500
9, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:4644)
9, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64)
9, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
9, 24 -- id KIM4AQ-000KDD-G8
9, 24 -- for Microscopy-at-microscopy.com; Fri, 24 Apr 2009 16:56:50 +0100
9, 24 -- Date: Fri, 24 Apr 2009 16:55:59 +0100
9, 24 -- To: Microscopy-at-microscopy.com
9, 24 -- Subject: Re: [Microscopy] Sections falling off grids
9, 24 -- Message-ID: {3641578467.1240592159-at-ls0130.lifesci.susx.ac.uk}
9, 24 -- In-Reply-To: {200904241518.n3OFIURB025961-at-ns.microscopy.com}
9, 24 -- Originator-Info: login-token=Mulberry:01+3BLc13RsdovsKvNZNQp48Dmb9W2mlzLKeW2;
9, 24 -- token_authority=postmaster-at-central.susx.ac.uk
9, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
9, 24 -- MIME-Version: 1.0
9, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed
9, 24 -- Content-Transfer-Encoding: 7bit
9, 24 -- Content-Disposition: inline
9, 24 -- X-Sussex: true
9, 24 -- X-Sussex-transport: remote_smtp_rew
9, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Fri, 24 Apr 2009 11:14:10 -0500
Subject: [Microscopy] Re: Re: Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Epon thin sections coming off naked grids during staining procedures was
solved when I followed Debby Sherman¹s suggestion of putting the grids
containing sections into my oven for at least 15 min. to overnight before
staining. I only need to do this occasionally since I do not have trouble
frequently (do not know why). I only use my oven for this treatment when it
is empty because I do not know if the fumes that are generated by curing
epoxy would have an effect on the grids even though they are either in a
closed Petri Dish on filter paper or in a Grid Box.

My newer grids (Electron Microscopy Sciences) do not need much cleaning but
old ones do. If you sonicate in Acetone to remove oil/grease residues I
found it advisable to do a final rinse in 100% ethanol before drying since I
think the Acetone sometimes leaves something behind on the grid surface.
This reduced the number of sections that I lost.

I have also used a very careful QUICK dip in HNO3 followed by several water
washes to make naked grids hydrophilic and hence make section pick-up from
the boat much easier. I had good luck with sections sticking where they
belonged with this technique. The problem was using the strong acid which
dissolved the grid if it was not washed quickly enough.

Wishing you good grids,
Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do
not represent the NIH. This message is not
confidential and can be freely shared and reproduced.

====================
X-from: {W.Muss-at-salk.at}

Good morning/good afternoon,
Hi all,

Jonathan, not having had such problems in the long years I'm doing my job
here I wonder about the } quality { and kind/type of resin sections you
usually are dealing with.

Please could you provide information about wether this is a problem without
a relationship to any or this is rellated to a specific resin type you use/
which is used.

Also it would be of interest wether you are talking about "falling off"
sections mounted on "naked" copper (standard) or other specific grids (like
nickel) or such one prefilmed with/by formvar/butvar/collodion film....

Thanking you and
best wishes and regards

Wolfgang MUSS
Salzburg, Austria

===========================================================
This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish some or
all of this message in the Microscopy Today journal.
==========================================================

} -----Ursprüngliche Nachricht-----
} Von: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
} Gesendet: Freitag, 24. April 2009 17:11
} An: Muß Wolfgang
} Betreff: [Microscopy] Sections falling off grids
}
} Hi:
}
} Every once and a while we run into problems with sections falling off the
grids. I know this has been rehashed more than once here, but its hard to find
the answers in the archives, so I am asking for the consolidated, final analysis
of the problem and solutions.
}
} I suspect it could be students using old, oxidized grids, but I'm not sure
enough to just give them that one answer.
}
} I have check on 'grid glues' and searched around, but knew the experts and
experienced would be here.
}
} So, what is your strategy. Never had the problem? Clean the grids with
solvents or acid, which ones?
} Grid glue or other solutions?
}
} Lay it on me baby.
}
} Jon
}
} Jonathan Krupp
} Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} 209-954-5284
} jkrupp-at-deltacollege.edu



==============================Original Headers==============================
18, 32 -- From connellyps-at-nhlbi.nih.gov Fri Apr 24 11:14:09 2009
18, 32 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113])
18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OGE8g2006300
18, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:14:09 -0500
18, 32 -- X-IronPortListener: Outbound_SMTP
18, 32 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115])
18, 32 -- by nihxway5out.hub.nih.gov with ESMTP; 24 Apr 2009 12:13:48 -0400
18, 32 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
18, 32 -- Fri, 24 Apr 2009 12:13:48 -0400
18, 32 -- Received: from NIHHTRC.nih.gov (156.40.71.74) by NIHHT01.nih.gov
18, 32 -- (156.40.71.20) with Microsoft SMTP Server (TLS) id 8.1.340.0; Fri, 24 Apr
18, 32 -- 2009 12:13:47 -0400
18, 32 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHTRC.nih.gov
18, 32 -- ([156.40.71.74]) with mapi; Fri, 24 Apr 2009 12:13:47 -0400
18, 32 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
18, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
18, 32 -- Date: Fri, 24 Apr 2009 12:12:03 -0400
18, 32 -- Subject: Re: [Microscopy] Re: Sections falling off grids
18, 32 -- Thread-Topic: [Microscopy] Re: Sections falling off grids
18, 32 -- Thread-Index: AcnE8eZu1Uhb/0O4QnSLGJa4eYH8nQABYEe8
18, 32 -- Message-ID: {C6175D13.34E9%connellyps-at-nhlbi.nih.gov}
18, 32 -- In-Reply-To: {200904241532.n3OFW3k3002378-at-ns.microscopy.com}
18, 32 -- Accept-Language: en, en-US
18, 32 -- Content-Language: en
18, 32 -- X-MS-Has-Attach:
18, 32 -- X-MS-TNEF-Correlator:
18, 32 -- acceptlanguage: en, en-US
18, 32 -- Content-Type: text/plain; charset="iso-8859-1"
18, 32 -- MIME-Version: 1.0
18, 32 -- X-OriginalArrivalTime: 24 Apr 2009 16:13:48.0309 (UTC) FILETIME=[A6516C50:01C9C4F7]
18, 32 -- Content-Transfer-Encoding: 8bit
18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OGE8g2006300
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Fri, 24 Apr 2009 11:22:33 -0500
Subject: [Microscopy] Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jonathan,
I agree that we should know more details, like if this is routine counterstaining or IHC. For basic counterstaining, a simple acid wash with proper rinsing and drying should be fine.

I have experienced highly variable section adhesion when I perform IHC with any Tween or Triton detergents, primarily when the grids sink into the reagent droplets (using square pattern, thin bar nickel grids). If this is your case, then I sympathize.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Friday, April 24, 2009 11:18 AM
To: Sobocinski, Gregg

Hi:

Every once and a while we run into problems with sections falling off
the grids. I know this has been rehashed more than once here, but its
hard to find the answers in the archives, so I am asking for the
consolidated, final analysis of the problem and solutions.

I suspect it could be students using old, oxidized grids, but I'm not
sure enough to just give them that one answer.

I have check on 'grid glues' and searched around, but knew the experts
and experienced would be here.

So, what is your strategy. Never had the problem? Clean the grids
with solvents or acid, which ones? Grid glue or other solutions?

Lay it on me baby.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009
11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500
11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT)
11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236])
11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT)
11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700
11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by
11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24
11, 42 -- Apr 2009 07:47:04 -0700
11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT)
11, 42 -- X-Virus-Scanned: amavisd-new at
11, 42 -- X-Spam-Flag: NO
11, 42 -- X-Spam-Score: -2.498
11, 42 -- X-Spam-Level:
11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000,
11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1]
11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ;
11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146])
11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT)
11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu}
11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
11, 42 -- To: microscopy-at-microscopy.com
11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 42 -- Content-Transfer-Encoding: 7bit
11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3)
11, 42 -- Subject: Sections falling off grids
11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700
11, 42 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================


==============================Original Headers==============================
19, 25 -- From greggps-at-umich.edu Fri Apr 24 11:22:31 2009
19, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OGMV45020493
19, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:22:31 -0500
19, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.234]) by
19, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Fri, 24 Apr
19, 25 -- 2009 12:22:31 -0400
19, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
19, 25 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
19, 25 -- Date: Fri, 24 Apr 2009 12:22:43 -0400
19, 25 -- Subject: RE: [Microscopy] Sections falling off grids
19, 25 -- Thread-Topic: [Microscopy] Sections falling off grids
19, 25 -- Thread-Index: AcnE79HCFgarVXm6QtGdLogOhbUECQAB3WNw
19, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA39340611560343-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
19, 25 -- References: {200904241517.n3OFHcl1024979-at-ns.microscopy.com}
19, 25 -- In-Reply-To: {200904241517.n3OFHcl1024979-at-ns.microscopy.com}
19, 25 -- Accept-Language: en-US
19, 25 -- Content-Language: en-US
19, 25 -- X-MS-Has-Attach:
19, 25 -- X-MS-TNEF-Correlator:
19, 25 -- acceptlanguage: en-US
19, 25 -- Content-Type: text/plain; charset="us-ascii"
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OGMV45020493
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 24 Apr 2009 11:23:07 -0500
Subject: [Microscopy] Sharpening carbon rods without saying bad words

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

File this Tip of the Week under "Okay, so why didn't I think of this
before, dummy?"

We have one of those hand-held carbon rod sharpeners that require you to
twist the rod manually in order to get the narrow tip needed for
evaporating. They are hard and uncomfortable to use, often break the
tip off just as you're finally getting it to the length you need, and
just generally a pain. But I could never bring myself to spring for the
hundreds of bucks for a decent sharpener, especially considering the
volume of evaporating we do.

As I was cranking away this morning, breaking tips and saying bad words,
I remembered that we had a Dremel tool in the next room. Took that
carbon rod, put it in the drill bit chuck, turned the tool on at its
lowest speed, inserted the rod into the sharpener and had my tip in
about 17 seconds.

Many of you probably had this one figured out a while ago, but if not,
here it is.

I now have the will to go on evaporating.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 27 -- From TindallR-at-missouri.edu Fri Apr 24 11:23:07 2009
10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OGN64h021629
10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:23:07 -0500
10, 27 -- X-IronPort-Anti-Spam-Filtered: true
10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAE6E8UnRauUo/2dsb2JhbADAIAEJhz+ITIJBgTMF
10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 24 Apr 2009 11:23:06 -0500
10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 27 -- Fri, 24 Apr 2009 11:23:06 -0500
10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 27 -- Content-class: urn:content-classes:message
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain;
10, 27 -- charset="us-ascii"
10, 27 -- Subject: Sharpening carbon rods without saying bad words
10, 27 -- Date: Fri, 24 Apr 2009 11:22:35 -0500
10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD808D-at-UM-XMAIL08.um.umsystem.edu}
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- Thread-Topic: Sharpening carbon rods without saying bad words
10, 27 -- Thread-Index: AcnE+OAKZMl5kF/XSr6NbxcPDeYSrg==
10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 27 -- To: {microscopy-at-microscopy.com}
10, 27 -- X-OriginalArrivalTime: 24 Apr 2009 16:23:06.0543 (UTC) FILETIME=[F30D2BF0:01C9C4F8]
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OGN64h021629
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Fri, 24 Apr 2009 12:24:43 -0500
Subject: [Microscopy] Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One thing missing from this thread is an explicit discussion of the
technique for picking up sections. I have always picked up sections from
below, and after 25 years and thousands of blocks, I have never had a
problem with sections falling off bare copper grids without adhesive, either
dull or shiny side, with Epon, Spurr's, or LR White. When you bring the
grid up from below, there is water between the section and the grid, and
when the water evaporates, the section becomes bonded tightly to the grid.
This is not so when you come from above.

I should add that I sonicate the grids in 100% ethanol then place them on
filter paper in a petri dish. If you have a problem with the sections
running away when you try to pick up sections, try dipping the grid briefly
in ethanol, then immediately rinse it thoroughly in distilled water, and
bring the wet grid to the boat. The purpose of this is not to clean the
grid, but to avoid the formation of tiny air bubbles on the grid, which tend
to repel the sections. You should also rotate the grid as you remove it
from the boat, so that it is vertical when it comes out of the water. This
avoids bringing up a large drop of water with the grid, and having your
sections shift when the water is blotted off.

Ralph Common
Michigan State Univ.


==============================Original Headers==============================
4, 24 -- From rcommon-at-msu.edu Fri Apr 24 12:24:42 2009
4, 24 -- Received: from sys50.mail.msu.edu (sys50.mail.msu.edu [35.9.75.230])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHOf4L017349
4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:24:42 -0500
4, 24 -- Received: from [35.9.122.125] (helo=emlab)
4, 24 -- by sys50.mail.msu.edu with esmtpsa (Exim 4.63 #12)
4, 24 -- (TLSv1:RC4-MD5:128)
4, 24 -- id 1LxP8i-0000e5-IZ
4, 24 -- for Microscopy-at-microscopy.com; Fri, 24 Apr 2009 13:24:40 -0400
4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
4, 24 -- To: {Microscopy-at-microscopy.com}
4, 24 -- Subject: Sections falling off grids
4, 24 -- Date: Fri, 24 Apr 2009 14:24:19 -0400
4, 24 -- Message-ID: {004101c9c509$e2c775f0$7d7a0923-at-msu.edu}
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: text/plain;
4, 24 -- charset="iso-8859-1"
4, 24 -- Content-Transfer-Encoding: 7bit
4, 24 -- X-Priority: 3 (Normal)
4, 24 -- X-MSMail-Priority: Normal
4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
4, 24 -- Importance: Normal
4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
4, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 24 Apr 2009 12:31:02 -0500
Subject: [Microscopy] Re: Sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} I have also used a very careful QUICK dip in HNO3 followed
} by several water washes to make naked grids hydrophilic and
} hence make section pick-up from the boat much easier. I had
} good luck with sections sticking where they belonged with
} this technique. The problem was using the strong acid which
} dissolved the grid if it was not washed quickly enough.
}
} Wishing you good grids,
} Pat

You do not need to use strong acid. 1N HCl can do job just fine. You can
keep a grid in this acid for a while without problems.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


==============================Original Headers==============================
6, 25 -- From DusevichV-at-umkc.edu Fri Apr 24 12:31:02 2009
6, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHV2as028473
6, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:31:02 -0500
6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Fri, 24 Apr 2009 12:31:02 -0500
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="US-ASCII"
6, 25 -- Subject: RE: [Microscopy] Re: Re: Sections falling off grids
6, 25 -- Date: Fri, 24 Apr 2009 12:31:01 -0500
6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB837-at-KC-MSX1.kc.umkc.edu}
6, 25 -- In-Reply-To: {200904241614.n3OGEfbV007158-at-ns.microscopy.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: [Microscopy] Re: Re: Sections falling off grids
6, 25 -- thread-index: AcnE98cJvPg9jbf/QoaeRWMd+68/pgACiUzA
6, 25 -- References: {200904241614.n3OGEfbV007158-at-ns.microscopy.com}
6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 24 Apr 2009 17:31:02.0238 (UTC) FILETIME=[705ADFE0:01C9C502]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OHV2as028473
==============================End of - Headers==============================




From: mark.grimson-at-ttu.edu
Date: Fri, 24 Apr 2009 12:54:42 -0500
Subject: [Microscopy] Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,
I, too, was plagued with sections falling off grids. Since I have been
following the following, I have not lost a single section!
Dip copper grid into a 0.1N solution of HCl (I usually count 10 seconds with
a drop of HCL on the grid) and blot dry.
Dip several times into 100% acetone to rinse and allow to dry on filter
paper.
I usually prepare the grids before I start sectioning so I don't have to
stop each time to get my grids ready. Once my sections are collected, I put
the grids into the oven for 20 minutes to dry. I can stain immediately after
removing the sections from the oven or wait until later, it doesn't seem to
matter.
Hope this helps your students! There is nothing worse than spending your
day sectioning and seeing only shreds on the TEM!
Take care,
Pat Kysar
University of California, Davis
Medical School, Pathology
EM Lab

----- Original Message -----
X-from: {connellyps-at-nhlbi.nih.gov}
To: {pekysar-at-ucdavis.edu}
Sent: Friday, April 24, 2009 9:20 AM

Hello. We have traditionally been a Life Sciences Imaging core facility on
campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
ourselves leaning more towards the Material Sciences. As such, we would
like to augment our Center's reference library with books relating to the
imaging and analytical side of microscopy

I was wondering if I could get ideas on basic book requirements that
Material scientists would look at as basic references and might be
considered indispensable in a Materials oriented research center. Thanks for
any help! Mark



Mark Grimson, PhD
Manager, The Imaging Center
c/o The Department of Biological Sciences
Flint and Main
Texas Tech University
Lubbock, TX 79409-3131

mark.grimson-at-ttu.edu
806-742-3722 x235 (Office)
806-252-3879 (Cell)
806-742-2963 (FAX)




==============================Original Headers==============================
9, 26 -- From mark.grimson-at-ttu.edu Fri Apr 24 12:54:42 2009
9, 26 -- Received: from eurymedon.net.ttu.edu (eurymedon.net.ttu.edu [129.118.1.225])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHsekZ028870
9, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:54:41 -0500
9, 26 -- Received: from hippolytus.net.ttu.edu (129.118.1.212) by tmrc.ttu.edu
9, 26 -- (129.118.1.179) with Microsoft SMTP Server id 8.1.358.0; Fri, 24 Apr 2009
9, 26 -- 12:54:38 -0500
9, 26 -- Received: from CARPO.ttu.edu ([129.118.1.206]) by hippolytus.net.ttu.edu
9, 26 -- ([129.118.1.212]) with mapi; Fri, 24 Apr 2009 12:54:38 -0500
9, 26 -- From: "Grimson, Mark" {mark.grimson-at-ttu.edu}
9, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
9, 26 -- Date: Fri, 24 Apr 2009 12:50:40 -0500
9, 26 -- Subject: Basic Materials TEM/SEM/ LM book ideas
9, 26 -- Thread-Topic: Basic Materials TEM/SEM/ LM book ideas
9, 26 -- Thread-Index: AcnFBS5bbRkWyDD4Ed6Y4QAWy6Vh7g==
9, 26 -- Message-ID: {C6176620.CAB7%mark.grimson-at-ttu.edu}
9, 26 -- Accept-Language: en-US
9, 26 -- Content-Language: en
9, 26 -- X-MS-Has-Attach:
9, 26 -- X-MS-TNEF-Correlator:
9, 26 -- acceptlanguage: en-US
9, 26 -- Content-Type: text/plain; charset="iso-8859-1"
9, 26 -- MIME-Version: 1.0
9, 26 -- X-TechMail-Edge-Route: TTU
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OHsekZ028870
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 24 Apr 2009 12:54:46 -0500
Subject: [Microscopy] RE: sectioning p4vp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can try something other than water to fill knife boat. I used
ethylene glycol for cutting mineralized cell cultures with some success.
It is toxic, easily wet block face, and evaporates much slower than
water (I kept grids overnight to let them dry out).
I have not idea about solubility of P4VP in ethylene glycol.
By the way, have you checked you grids with TEM? Sometimes, when
floating in a boat, embedded specimen could be transparent (invisible),
while resin around it had usual silver or gold color.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: leolukman-at-wisc.edu [mailto:leolukman-at-wisc.edu]
} Sent: Thursday, April 23, 2009 1:22 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] sectioning p4vp
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi everyone,
}
} I'm a graduate student at Materials Science Program. I tried
} to section a poly(4-vinylpyridine) (P4VP) embedded in epon
} with a diamond knife and water on the boat. However, it
} seemed that the water ate away my sample and the knife only
} cut the epon. If i looked at the sample face after trying to
} section, i could see that the sample face had become rough,
} which i assumed because the water somehow dissolving the
} sample (In large amount, P4VP is insoluble in water). Would
} you please suggest me how to resolve this problem? Thank you
} very much.
}
} Sincerely,
} Melvina Leolukman
}
} ==============================Original
} Headers==============================
} 3, 33 -- From leolukman-at-wisc.edu Thu Apr 23 13:21:09 2009 3,
} 33 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu
} [144.92.197.210])
} 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n3NIL85O027207
} 3, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr
} 2009 13:21:08 -0500
} 3, 33 -- MIME-version: 1.0
} 3, 33 -- Content-transfer-encoding: 7BIT 3, 33 --
} Content-disposition: inline 3, 33 -- Content-type:
} text/plain; charset=us-ascii 3, 33 -- Received: from
} avs-daemon.smtpauth1.wiscmail.wisc.edu by 3, 33 --
} smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System
} Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 --
} id {0KIK00E02GB8KN00-at-smtpauth1.wiscmail.wisc.edu} for 3, 33
} -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:08
} -0500 (CDT) 3, 33 -- Received: from wiscmail.wisc.edu
} (store1.doit.wisc.edu [144.92.8.164]) 3, 33 -- by
} smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System
} Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 --
} with ESMTP id {0KIK009W9GB76120-at-smtpauth1.wiscmail.wisc.edu}
} for 3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009
} 13:21:07 -0500 (CDT) 3, 33 -- Received: from [144.92.8.222]
} (Forwarded-For: 128.104.185.23, [144.92.197.247]) 3, 33 --
} by store1.doit.wisc.edu (mshttpd); Thu, 23 Apr 2009 13:21:07
} -0500 3, 33 -- Date: Thu, 23 Apr 2009 13:21:07 -0500 3, 33 --
} From: MELVINA LEOLUKMAN {leolukman-at-wisc.edu} 3, 33 --
} Subject: sectioning p4vp 3, 33 -- To:
} Microscopy-at-microscopy.com 3, 33 -- Message-id:
} {e51c89d31f6ec.49f06b43-at-wiscmail.wisc.edu}
} 3, 33 -- X-Mailer: Sun Java(tm) System Messenger Express
} 6.2-8.04 (built Feb 28 2007) 3, 33 -- Content-language: en 3,
} 33 -- X-Accept-Language: en 3, 33 -- Priority: normal 3, 33
} -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.164 3,
} 33 -- X-Spam-PmxInfo: Server=avs-10, Version=5.5.1.360522, 3,
} 33 -- Antispam-Engine: 2.6.1.350677, Antispam-Data:
} 2009.4.23.180432, 3, 33 -- SenderIP=144.92.8.164
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Fri Apr 24 12:54:45 2009
7, 25 -- Received: from kc-msxproto1.kc.umkc.edu (exchange.umkc.edu [134.193.143.167])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHshSW028893
7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:54:44 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Fri, 24 Apr 2009 12:54:42 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="US-ASCII"
7, 25 -- Subject: RE: [Microscopy] sectioning p4vp
7, 25 -- Date: Fri, 24 Apr 2009 12:54:41 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB838-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200904231822.n3NIMSL3028113-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] sectioning p4vp
7, 25 -- thread-index: AcnEQHeCBGxAvokiRn6Wcw1dqRRKbQAwzd0Q
7, 25 -- References: {200904231822.n3NIMSL3028113-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {leolukman-at-wisc.edu} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 24 Apr 2009 17:54:42.0908 (UTC) FILETIME=[BF23E9C0:01C9C505]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OHshSW028893
==============================End of - Headers==============================




From: Jacqueline.Ayotte-at-ticona.com
Date: Fri, 24 Apr 2009 13:38:52 -0500
Subject: [Microscopy] Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Mark,

Polymer microscopy is my life. I would recommend the most recent edition
of Polymer Microscopy by Linda C. Sawyer and David T. Grubb. You
probably already have Scanning Electron Microscopy and X-Ray
Microanalysis by Goldstein, Newbury, Joy et. al. It is not solely about
materials but does cover them and I believe an indispensable book.

Regards,
Jackie

Jacqueline Ayotte
Microscopist - Advanced Materials Characterization
Ticona
8040 Dixie Highway
} Florence KY 41042
} 859-372-3139
} fax 859-372-3184
} jacqueline.ayotte-at-ticona.com
The information contained in this e-mail, and any attachments thereto,
is confidential and is intended only for use by the individual(s) and/or
entity named above. If you are not the intended recipient of this
e-mail, you are hereby notified that any dissemination, distribution or
copying of this communication or any disclosure of the contents of this
communication to others is strictly prohibited. If you have received
this communication in error, please notify the sender immediately by
replying to this e-mail. Please then delete the original including all
attachments and any copy of any e-mail and printout thereof.



-----Original Message-----
X-from: mark.grimson-at-ttu.edu [mailto:mark.grimson-at-ttu.edu]
Sent: Friday, April 24, 2009 2:03 PM
To: Ayotte, Jacqueline M., Ticona/US

Hello. We have traditionally been a Life Sciences Imaging core facility
on campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
ourselves leaning more towards the Material Sciences. As such, we would
like to augment our Center's reference library with books relating to
the imaging and analytical side of microscopy

I was wondering if I could get ideas on basic book requirements that
Material scientists would look at as basic references and might be
considered indispensable in a Materials oriented research center. Thanks
for any help! Mark



Mark Grimson, PhD
Manager, The Imaging Center
c/o The Department of Biological Sciences Flint and Main Texas Tech
University
Lubbock, TX 79409-3131

mark.grimson-at-ttu.edu
806-742-3722 x235 (Office)
806-252-3879 (Cell)
806-742-2963 (FAX)




==============================Original
Headers==============================
9, 26 -- From mark.grimson-at-ttu.edu Fri Apr 24 12:54:42 2009 9, 26 --
Received: from eurymedon.net.ttu.edu (eurymedon.net.ttu.edu
[129.118.1.225])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n3OHsekZ028870
9, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009
12:54:41 -0500
9, 26 -- Received: from hippolytus.net.ttu.edu (129.118.1.212) by
tmrc.ttu.edu 9, 26 -- (129.118.1.179) with Microsoft SMTP Server id
8.1.358.0; Fri, 24 Apr 2009 9, 26 -- 12:54:38 -0500 9, 26 -- Received:
from CARPO.ttu.edu ([129.118.1.206]) by hippolytus.net.ttu.edu 9, 26 --
([129.118.1.212]) with mapi; Fri, 24 Apr 2009 12:54:38 -0500 9, 26 --
X-from: "Grimson, Mark" {mark.grimson-at-ttu.edu} 9, 26 -- To:
"Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 26 -- Date:
Fri, 24 Apr 2009 12:50:40 -0500 9, 26 -- Subject: Basic Materials
TEM/SEM/ LM book ideas 9, 26 -- Thread-Topic: Basic Materials TEM/SEM/
LM book ideas 9, 26 -- Thread-Index: AcnFBS5bbRkWyDD4Ed6Y4QAWy6Vh7g== 9,
26 -- Message-ID: {C6176620.CAB7%mark.grimson-at-ttu.edu}
9, 26 -- Accept-Language: en-US
9, 26 -- Content-Language: en
9, 26 -- X-MS-Has-Attach:
9, 26 -- X-MS-TNEF-Correlator:
9, 26 -- acceptlanguage: en-US
9, 26 -- Content-Type: text/plain; charset="iso-8859-1"
9, 26 -- MIME-Version: 1.0
9, 26 -- X-TechMail-Edge-Route: TTU
9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted:
from quoted-printable to 8bit by ns.microscopy.com id n3OHsekZ028870
==============================End of -
Headers==============================


==============================Original Headers==============================
21, 31 -- From Jacqueline.Ayotte-at-ticona.com Fri Apr 24 13:38:08 2009
21, 31 -- Received: from exprod7og122.obsmtp.com (exprod7og122.obsmtp.com [64.18.2.22])
21, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OIc7AA026135
21, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 13:38:07 -0500
21, 31 -- Received: from source ([148.163.79.15]) by exprod7ob122.postini.com ([64.18.6.12]) with SMTP
21, 31 -- ID DSNKSfIHDsSu1r2EyC0Sz2VmZ8SHyI9Eqq7J-at-postini.com; Fri, 24 Apr 2009 11:38:07 PDT
21, 31 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp2.celanese.com with Microsoft SMTPSVC(6.0.3790.3959);
21, 31 -- Fri, 24 Apr 2009 13:38:06 -0500
21, 31 -- Received: from amalnxmbx1.SW.CZDS.BZ ([148.163.82.91]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
21, 31 -- Fri, 24 Apr 2009 13:38:06 -0500
21, 31 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxmbx1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959);
21, 31 -- Fri, 24 Apr 2009 13:38:06 -0500
21, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 31 -- Content-class: urn:content-classes:message
21, 31 -- MIME-Version: 1.0
21, 31 -- Content-Type: text/plain;
21, 31 -- charset="us-ascii"
21, 31 -- Subject: RE: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
21, 31 -- Date: Fri, 24 Apr 2009 14:38:03 -0400
21, 31 -- Message-ID: {A2D45D1AE135A5418A4D38183241688F051514-at-amfloxmbx1.SW.CZDS.BZ}
21, 31 -- In-Reply-To: {200904241803.n3OI3KgK023071-at-ns.microscopy.com}
21, 31 -- X-MS-Has-Attach:
21, 31 -- X-MS-TNEF-Correlator:
21, 31 -- Thread-Topic: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
21, 31 -- thread-index: AcnFBvSToOLBaFRxR/qOQIWp00CNNAAAxsZA
21, 31 -- References: {200904241803.n3OI3KgK023071-at-ns.microscopy.com}
21, 31 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com}
21, 31 -- To: {Microscopy-at-microscopy.com} , {mark.grimson-at-ttu.edu}
21, 31 -- X-OriginalArrivalTime: 24 Apr 2009 18:38:06.0141 (UTC) FILETIME=[CEC9BED0:01C9C50B]
21, 31 -- Content-Transfer-Encoding: 8bit
21, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OIc7AA026135
==============================End of - Headers==============================




From: gas19-at-chrysler.com
Date: 04/24/2009 02:01PM
Subject: [Microscopy] Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You should start with some books from ASM (American Society of Materials).
Two basic books would be Volume 9 of the ASM Handbook, Metallography and
Microstructures (LM of Metals) and Volume 11 of the ASM Handbook Failure
Analysis and Prevention (SEM). It depends on how extensive you want to be
in your library. There are many other good books, but those are two good
books to start with.


Gerald Shulke
Materials Engineering Specialist
Chrysler LLC


-----mark.grimson-at-ttu.edu wrote: -----


To: gas19-at-chrysler.com
X-from: mark.grimson-at-ttu.edu




----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Hello. We have traditionally been a Life Sciences Imaging core facility on
campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
ourselves leaning more towards the Material Sciences. As such, we would
like to augment our Center's reference library with books relating to the
imaging and analytical side of microscopy

I was wondering if I could get ideas on basic book requirements that
Material scientists would look at as basic references and might be
considered indispensable in a Materials oriented research center. Thanks
for
any help! Mark



Mark Grimson, PhD
Manager, The Imaging Center
c/o The Department of Biological Sciences
Flint and Main
Texas Tech University
Lubbock, TX 79409-3131

mark.grimson-at-ttu.edu
806-742-3722 x235 (Office)
806-252-3879 (Cell)
806-742-2963 (FAX)




==============================Original
Headers==============================
9, 26 -- From mark.grimson-at-ttu.edu Fri Apr 24 12:54:42 2009
9, 26 -- Received: from eurymedon.net.ttu.edu (eurymedon.net.ttu.edu
[129.118.1.225])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3OHsekZ028870
9, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:54:41
-0500
9, 26 -- Received: from hippolytus.net.ttu.edu (129.118.1.212) by
tmrc.ttu.edu
9, 26 -- (129.118.1.179) with Microsoft SMTP Server id 8.1.358.0; Fri, 24
Apr 2009
9, 26 -- 12:54:38 -0500
9, 26 -- Received: from CARPO.ttu.edu ([129.118.1.206]) by
hippolytus.net.ttu.edu
9, 26 -- ([129.118.1.212]) with mapi; Fri, 24 Apr 2009 12:54:38 -0500
9, 26 -- From: "Grimson, Mark" {mark.grimson-at-ttu.edu}
9, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
9, 26 -- Date: Fri, 24 Apr 2009 12:50:40 -0500
9, 26 -- Subject: Basic Materials TEM/SEM/ LM book ideas
9, 26 -- Thread-Topic: Basic Materials TEM/SEM/ LM book ideas
9, 26 -- Thread-Index: AcnFBS5bbRkWyDD4Ed6Y4QAWy6Vh7g==
9, 26 -- Message-ID: {C6176620.CAB7%mark.grimson-at-ttu.edu}
9, 26 -- Accept-Language: en-US
9, 26 -- Content-Language: en
9, 26 -- X-MS-Has-Attach:
9, 26 -- X-MS-TNEF-Correlator:
9, 26 -- acceptlanguage: en-US
9, 26 -- Content-Type: text/plain; charset="iso-8859-1"
9, 26 -- MIME-Version: 1.0
9, 26 -- X-TechMail-Edge-Route: TTU
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n3OHsekZ028870
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 29 -- From gas19-at-chrysler.com Fri Apr 24 13:39:00 2009
24, 29 -- Received: from odbmap01.extra.chrysler.com (odbmap01.out.extra.chrysler.com [129.9.40.25])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OIcxZT026312
24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 13:38:59 -0500
24, 29 -- Received: from shmsp085.shdc.chrysler.com (unknown [129.9.158.254])
24, 29 -- by odbmap01.extra.chrysler.com (Symantec Mail Security) with ESMTP id A5CBB4E4003
24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:58 -0400 (EDT)
24, 29 -- X-AuditID: 81092818-a52f7bb0000060c3-f7-49f2074212ee
24, 29 -- Received: from sodddm23.wk.dcx.com (shmsp073-vip1.shdc.chrysler.com [53.231.161.238])
24, 29 -- by shmsp085.shdc.chrysler.com (Symantec Mail Security) with ESMTP id F15654A11
24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:25 -0400 (EDT)
24, 29 -- Importance: Normal
24, 29 -- X-Priority: 3 (Normal)
24, 29 -- In-Reply-To: {200904241801.n3OI1rPH018203-at-ns.microscopy.com}
24, 29 -- References: {200904241801.n3OI1rPH018203-at-ns.microscopy.com}
24, 29 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
24, 29 -- MIME-Version: 1.0
24, 29 -- From: gas19-at-chrysler.com
24, 29 -- To: microscopy-at-microscopy.com
24, 29 -- X-MIMETrack: MIME-CD by Notes Server on sctcdm03.wk.dcx.com/Server/Prod/DCX(Release
24, 29 -- 8.0.2HF127 | October 9, 2008) at 04/24/2009 14:39:45,
24, 29 -- MIME-CD complete at 04/24/2009 14:39:45,
24, 29 -- Serialize by Router on sodddm23.wk.dcx.com/Server/Prod/DCX(Release
24, 29 -- 8.0.2HF887 | March 23, 2009) at 04/24/2009 14:38:28
24, 29 -- Message-ID: {OF07F32566.0886C74D-ON852575A2.00668479-852575A2.0066847E-at-wkamerica.notes.chrysler.com}
24, 29 -- Date: Fri, 24 Apr 2009 14:39:45 -0400
24, 29 -- X-Mailer: Lotus Domino Web Server Release 8.0.2HF127 October 9, 2008
24, 29 -- Content-type: text/plain; charset=US-ASCII
24, 29 -- X-Brightmail-Tracker: AAAAAA==
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 24 Apr 2009 14:07:19 -0500
Subject: [Microscopy] Re: Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7JD3022539
for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:19 -0500
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n3OJ7JRN022537;
Fri, 24 Apr 2009 14:07:19 -0500

Hi Mark,

TEM: Willilams & Carter, "Transmission Electron Microscopy" New
editioin due this June
Reimer & Kohl, "Transmission Electron Microscopy: Physics
of Image Formation (Springer Series in Optical Sciences)"
SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray
Microanalysis" get the latest edition -- 3rd, I think
Reimer, "Scanning Electron Microscopy: Physics of Image
Formation and Microanalysis (Springer Series in Optical Sciences"
1998, though.
General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition
For a start.
What I have a hard time finding is a reference on specimen
preparation for materials science. Be interesting to see if anyone
posts such a book.

Phil

} Hello. We have traditionally been a Life Sciences Imaging core facility on
} campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
} ourselves leaning more towards the Material Sciences. As such, we would
} like to augment our Center's reference library with books relating to the
} imaging and analytical side of microscopy
}
} I was wondering if I could get ideas on basic book requirements that
} Material scientists would look at as basic references and might be
} considered indispensable in a Materials oriented research center. Thanks for
} any help! Mark
}
}
}
} Mark Grimson, PhD
} Manager, The Imaging Center
} c/o The Department of Biological Sciences
} Flint and Main
} Texas Tech University
} Lubbock, TX 79409-3131
}
} mark.grimson-at-ttu.edu
} 806-742-3722 x235 (Office)
} 806-252-3879 (Cell)
} 806-742-2963 (FAX)
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 23 -- From oshel1pe-at-cmich.edu Fri Apr 24 14:07:18 2009
4, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7IKc022534
4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:18 -0500
4, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3OJ7Ivo011624
4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:07:18 -0400
4, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 23 -- Fri, 24 Apr 2009 15:07:17 -0400
4, 23 -- Mime-Version: 1.0
4, 23 -- Message-Id: {f06240816c617be48e46a-at-[141.209.160.249]}
4, 23 -- Date: Fri, 24 Apr 2009 15:07:11 -0400
4, 23 -- To: Microscopy-at-microscopy.com
4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 23 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 23 -- X-OriginalArrivalTime: 24 Apr 2009 19:07:18.0060 (UTC) FILETIME=[E3035EC0:01C9C50F]
4, 23 -- X-Canit-CHI2: 0.00
4, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 23 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 23 -- X-CanItPRO-Stream: default
4, 23 -- X-Canit-Stats-ID: 12386593 - 56aaaf500e5c
4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 24 Apr 2009 15:21:22 -0500
Subject: [Microscopy] Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pat, Jon, and Microlisters,

My technique for cleaning grids is a variation
on Pat's method, by using acetone and HCl together.

I mix up a 250 ml lot of cleaning solution as
follows: 25 ml conc. HCl, 175 ml distilled
water, 50 ml acetone(99%). Of course, add the
acid to the water, then add the acetone.

I sonicate copper or nickel grids, mesh or
slots, for about 30 seconds in a 25 ml beaker
with about 10 ml solution. Then I pour that off,
and sonicate once with 99% acetone as a rinse.
Then invert beaker onto clean filter paper to
air dry. The grids usually stick a bit to inside
of beaker but will fall off when they dry.

I do this each day before I begin sectioning.
The copper grids get so clean - fresh copper
exposed - that they may oxidize enough over
night to need cleaning again even just a day
later. I also clean grids this way before any
coating with films, like Formvar or Butvar.

This method of cleaning has worked for me for
many years and sections stick to the grid.

Oh, I also pick up floating sections from above
onto the dull side of the grids. Others report
good results picking up from below the floating
sections, or onto the shiny side of the grids.
Others report good results cleaning grids by
quickly flaming them in an alcohol lamp flame.

In this new age of solar energy, I have not yet
tried cleaning grids by concentrating the suns
rays with a 4" hand lens onto grids resting on a
clean, refractory surface. But if it worked,
that would be soooo green! Someone ought to try
this.

Hey - whatever works babe, like, whatever!!


Gib Ahlstrand
Imaging Center
University of Minnesota
123 Snyder Hall
St. Paul, MN 55108
http://cbs.umn.edu/ic/

This message sent from my Mac Power PC G4 400
MHz beast! Grrrrr!
---------------------------------

-------- Original Message --------

Hi Jon,
I, too, was plagued with sections falling off
grids. Since I have been
following the following, I have not lost a
single section!
Dip copper grid into a 0.1N solution of HCl (I
usually count 10 seconds with
a drop of HCL on the grid) and blot dry.
Dip several times into 100% acetone to rinse and
allow to dry on filter
paper.
I usually prepare the grids before I start
sectioning so I don't have to
stop each time to get my grids ready. Once my
sections are collected, I put
the grids into the oven for 20 minutes to dry. I
can stain immediately after
removing the sections from the oven or wait
until later, it doesn't seem to
matter.
Hope this helps your students! There is nothing
worse than spending your
day sectioning and seeing only shreds on the TEM!
Take care,
Pat Kysar
University of California, Davis
Medical School, Pathology
EM Lab

----- Original Message -----
X-from: {connellyps-at-nhlbi.nih.gov}
To: {pekysar-at-ucdavis.edu}
Sent: Friday, April 24, 2009 9:20 AM

Gerald,

I'm looking for a general (" ") sample prep book that discusses the
"how to", "why to", and wherefores of the various methods --
ion-milling, etching, tripod polishing, small-angle cleavage, etc. --
for a variety of different kinds of materials. Naturally, all this
varies with the sample type and microscopy, as well as with the
questions asked.

So, not a specific, say, fractography book, or thin-film book, or
even more-general-but-still-specific book such as for metallurgy, but
a text like one would use in a materials EM course, but concentrated
on sample prep, as opposed to a chapter on sample prep in a more
general EM text. Which would also be handy to have hanging around the
facility when whoever walks in with whatever kind of sample they have.
Like we have in biology.

Phil

Phil,

Sample prep, well, I can say it depends....

What type of samples are you interested in? Metals, plastics, or
ceramics? Are you more interested in light microscopy or electron
microscopy?

Basically we use microscopy for two things: materials
characterization and failure analysis. For materials
characterization there are a number of protocols depending on the
material and what you are looking for in that material. For metals,
let's say, it depends on the alloy, how it was processed, and what
phases you are looking for. Most sample are mounted in Bakelite or
epoxy, ground, polished, and etched to reveal the microstructure.
For failure analysis, there is not much sample prep. Usually you
are looking for contaminants, deposits, etc. so you look at the
sample in the as received condition first. After you have collected
what information you can, then you clean the sample to see the
fracture surface topography. For metals, it may be acetone or
hexanes to remove oil and dirt. There are more aggressive
approaches, but the idea is to remove most of the surface
contamination without damaging the underlying material. It depends
on if the sample was corroded or not. For polymers, the surface may
be cleaned with soapy water. Anything harsher can destroy the
surface. If the material is not very conductive for SEM, then we
gold sputter coat the sample. Usually this is a last resort, because
you can't take it back off. You can do a lot with low voltage or in
an ESEM.

I can go into more detail if you want. I can post to the server to
share with everyone if you could tell me more specifically what you
are after. There are numerous books, especially for metals.

Gerald Shulke
Materials Engineering Specialist
Chrysler LLC

Hi Mark,

TEM: Willilams & Carter, "Transmission Electron Microscopy" New
editioin due this June
Reimer & Kohl, "Transmission Electron Microscopy: Physics
of Image Formation (Springer Series in Optical Sciences)"
SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray
Microanalysis" get the latest edition -- 3rd, I think
Reimer, "Scanning Electron Microscopy: Physics of Image
Formation and Microanalysis (Springer Series in Optical Sciences"
1998, though.
General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition
For a start.
What I have a hard time finding is a reference on specimen
preparation for materials science. Be interesting to see if anyone
posts such a book.

Phil

} Hello. We have traditionally been a Life Sciences Imaging core facility on
} campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
} ourselves leaning more towards the Material Sciences. As such, we would
} like to augment our Center's reference library with books relating to the
} imaging and analytical side of microscopy
}
} I was wondering if I could get ideas on basic book requirements that
} Material scientists would look at as basic references and might be
} considered indispensable in a Materials oriented research center. Thanks for
} any help! Mark
}
}
}
} Mark Grimson, PhD
} Manager, The Imaging Center
} c/o The Department of Biological Sciences
} Flint and Main
} Texas Tech University
} Lubbock, TX 79409-3131
}
} mark.grimson-at-ttu.edu
} 806-742-3722 x235 (Office)
} 806-252-3879 (Cell)
} 806-742-2963 (FAX)
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
15, 30 -- From oshel1pe-at-cmich.edu Fri Apr 24 15:21:21 2009
15, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OKLKup020898
15, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:21:20 -0500
15, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74])
15, 30 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3OKLIJ4031252;
15, 30 -- Fri, 24 Apr 2009 16:21:18 -0400
15, 30 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
15, 30 -- Fri, 24 Apr 2009 16:21:18 -0400
15, 30 -- Mime-Version: 1.0
15, 30 -- Message-Id: {f06240817c617cd2760b7-at-[141.209.160.249]}
15, 30 -- In-Reply-To:
15, 30 -- {OFDCC852DF.76D26DD5-ON852575A2.006BA084-852575A2.006D9E7D-at-wkamerica.notes
15, 30 -- .chrysler.com}
15, 30 -- References:
15, 30 -- {OFDCC852DF.76D26DD5-ON852575A2.006BA084-852575A2.006D9E7D-at-wkamerica.notes
15, 30 -- .chrysler.com}
15, 30 -- Date: Fri, 24 Apr 2009 16:21:15 -0400
15, 30 -- To: Microscopy-at-microscopy.com
15, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
15, 30 -- Subject: Re: [Microscopy] Re: Basic Materials TEM/SEM/ LM book ideas
15, 30 -- Cc: gas19-at-chrysler.com
15, 30 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
15, 30 -- X-OriginalArrivalTime: 24 Apr 2009 20:21:18.0279 (UTC) FILETIME=[39970570:01C9C51A]
15, 30 -- X-Canit-CHI2: 0.00
15, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
15, 30 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
15, 30 -- X-CanItPRO-Stream: default
15, 30 -- X-Canit-Stats-ID: 12390427 - 447ae406154e
15, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Fri, 24 Apr 2009 15:25:12 -0500
Subject: [Microscopy] HT cable wanted for Zeiss EM10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our high voltage cable went bad on our little old Zeiss EM10, vintage
1976. Would anyone have a spare?
Can you recommend a company to make a new one?
Thanks,

Roseann Csencsits, PhD
Scientist in Charge - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548

==============================Original Headers==============================
2, 23 -- From RCsencsits-at-lbl.gov Fri Apr 24 15:25:11 2009
2, 23 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45])
2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OKPBAX028666
2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:25:11 -0500
2, 23 -- X-Ironport-SBRS: 2.3
2, 23 -- X-IronPort-Anti-Spam-Filtered: true
2, 23 -- X-IronPort-Anti-Spam-Result: Aj4CAIO88UmAAykYe2dsb2JhbACWTgEBFiIFqUkJj3aCQYEzBQ
2, 23 -- X-IronPort-AV: E=Sophos;i="4.40,243,1239001200";
2, 23 -- d="scan'208";a="12586616"
2, 23 -- Received: from mta1.lbl.gov ([128.3.41.24])
2, 23 -- by ironport4.lbl.gov with ESMTP; 24 Apr 2009 13:25:10 -0700
2, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246])
2, 23 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n3OKPADO025737
2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Apr 2009 13:25:10 -0700 (PDT)
2, 23 -- Message-Id: {EF4BAEF5-F5E7-49C1-B756-C1AB159127BF-at-lbl.gov}
2, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
2, 23 -- To: Microscopy-at-microscopy.com
2, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
2, 23 -- Content-Transfer-Encoding: 7bit
2, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
2, 23 -- Subject: HT cable wanted for Zeiss EM10
2, 23 -- Date: Fri, 24 Apr 2009 13:25:10 -0700
2, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: gas19-at-chrysler.com
Date: 04/24/2009 04:27PM
Subject: [Microscopy] Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll refer to my other post for good general books for material science for
both LM and SEM. I do not know of a specific book for sample prep for SEM
in the materials field. There is an older book "SEM: A User's Manual for
Material Science" by Gabriel, also published by ASM, but it is from the
80's and somewhat outdated. Sample prep techniques for SEM is usually
covered in a chapter or a general discussion in several books based on
failure analysis or specific for a type of material. For general sample
prep of metals for LM, I would add "Metallography Principles and Practice"
by George Vander Voort.

Gerald Shulke
Materials Specialist
Chrysler LLC



-----oshel1pe-at-cmich.edu wrote: -----


To: gas19-at-chrysler.com
X-from: oshel1pe-at-cmich.edu




----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Gerald,

I'm looking for a general (" ") sample prep book that discusses the
"how to", "why to", and wherefores of the various methods --
ion-milling, etching, tripod polishing, small-angle cleavage, etc. --
for a variety of different kinds of materials. Naturally, all this
varies with the sample type and microscopy, as well as with the
questions asked.

So, not a specific, say, fractography book, or thin-film book, or
even more-general-but-still-specific book such as for metallurgy, but
a text like one would use in a materials EM course, but concentrated
on sample prep, as opposed to a chapter on sample prep in a more
general EM text. Which would also be handy to have hanging around the
facility when whoever walks in with whatever kind of sample they have.
Like we have in biology.

Phil

Phil,

Sample prep, well, I can say it depends....

What type of samples are you interested in? Metals, plastics, or
ceramics? Are you more interested in light microscopy or electron
microscopy?

Basically we use microscopy for two things: materials
characterization and failure analysis. For materials
characterization there are a number of protocols depending on the
material and what you are looking for in that material. For metals,
let's say, it depends on the alloy, how it was processed, and what
phases you are looking for. Most sample are mounted in Bakelite or
epoxy, ground, polished, and etched to reveal the microstructure.
For failure analysis, there is not much sample prep. Usually you
are looking for contaminants, deposits, etc. so you look at the
sample in the as received condition first. After you have collected
what information you can, then you clean the sample to see the
fracture surface topography. For metals, it may be acetone or
hexanes to remove oil and dirt. There are more aggressive
approaches, but the idea is to remove most of the surface
contamination without damaging the underlying material. It depends
on if the sample was corroded or not. For polymers, the surface may
be cleaned with soapy water. Anything harsher can destroy the
surface. If the material is not very conductive for SEM, then we
gold sputter coat the sample. Usually this is a last resort, because
you can't take it back off. You can do a lot with low voltage or in
an ESEM.

I can go into more detail if you want. I can post to the server to
share with everyone if you could tell me more specifically what you
are after. There are numerous books, especially for metals.

Gerald Shulke
Materials Engineering Specialist
Chrysler LLC

Hi Mark,

TEM: Willilams & Carter, "Transmission Electron Microscopy" New
editioin due this June
Reimer & Kohl, "Transmission Electron Microscopy: Physics
of Image Formation (Springer Series in Optical Sciences)"
SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray
Microanalysis" get the latest edition -- 3rd, I think
Reimer, "Scanning Electron Microscopy: Physics of Image
Formation and Microanalysis (Springer Series in Optical Sciences"
1998, though.
General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition
For a start.
What I have a hard time finding is a reference on specimen
preparation for materials science. Be interesting to see if anyone
posts such a book.

Phil

} Hello. We have traditionally been a Life Sciences Imaging core facility
on
} campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
} ourselves leaning more towards the Material Sciences. As such, we would
} like to augment our Center's reference library with books relating to the
} imaging and analytical side of microscopy
}
} I was wondering if I could get ideas on basic book requirements that
} Material scientists would look at as basic references and might be
} considered indispensable in a Materials oriented research center. Thanks
for
} any help! Mark
}
}
}
} Mark Grimson, PhD
} Manager, The Imaging Center
} c/o The Department of Biological Sciences
} Flint and Main
} Texas Tech University
} Lubbock, TX 79409-3131
}
} mark.grimson-at-ttu.edu
} 806-742-3722 x235 (Office)
} 806-252-3879 (Cell)
} 806-742-2963 (FAX)
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original
Headers==============================
15, 30 -- From oshel1pe-at-cmich.edu Fri Apr 24 15:21:21 2009
15, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3OKLKup020898
15, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:21:20
-0500
15, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74])
15, 30 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id
n3OKLIJ4031252;
15, 30 -- Fri, 24 Apr 2009 16:21:18 -0400
15, 30 -- Received: from [141.209.160.249] ([141.209.160.249]) by
egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
15, 30 -- Fri, 24 Apr 2009 16:21:18 -0400
15, 30 -- Mime-Version: 1.0
15, 30 -- Message-Id: {f06240817c617cd2760b7-at-[141.209.160.249]}
15, 30 -- In-Reply-To:
15, 30 --
{OFDCC852DF.76D26DD5-ON852575A2.006BA084-852575A2.006D9E7D-at-wkamerica.notes
15, 30 -- .chrysler.com}
15, 30 -- References:
15, 30 --
{OFDCC852DF.76D26DD5-ON852575A2.006BA084-852575A2.006D9E7D-at-wkamerica.notes
15, 30 -- .chrysler.com}
15, 30 -- Date: Fri, 24 Apr 2009 16:21:15 -0400
15, 30 -- To: Microscopy-at-microscopy.com
15, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
15, 30 -- Subject: Re: [Microscopy] Re: Basic Materials TEM/SEM/ LM book
ideas
15, 30 -- Cc: gas19-at-chrysler.com
15, 30 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
15, 30 -- X-OriginalArrivalTime: 24 Apr 2009 20:21:18.0279 (UTC) FILETIME=
[39970570:01C9C51A]
15, 30 -- X-Canit-CHI2: 0.00
15, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
15, 30 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes
(0.0001,-0.5)
15, 30 -- X-CanItPRO-Stream: default
15, 30 -- X-Canit-Stats-ID: 12390427 - 447ae406154e
15, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of -
Headers==============================


==============================Original Headers==============================
30, 29 -- From gas19-at-chrysler.com Fri Apr 24 16:25:05 2009
30, 29 -- Received: from shbmap01.extra.chrysler.com (shbmap01.out.extra.chrysler.com [129.9.168.35])
30, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OLP5DB018459
30, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 16:25:05 -0500
30, 29 -- Received: from odmsp099.oddc.chrysler.com (unknown [129.9.32.254])
30, 29 -- by shbmap01.extra.chrysler.com (Symantec Mail Security) with ESMTP id A5FE14E4002
30, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 17:25:04 -0400 (EDT)
30, 29 -- X-AuditID: 8109a822-abedfbb000000bc0-54-49f22e30b603
30, 29 -- Received: from sodddm23.wk.dcx.com (shmsp073-vip1.shdc.chrysler.com [53.231.161.238])
30, 29 -- by odmsp099.oddc.chrysler.com (Symantec Mail Security) with ESMTP id 347661E31
30, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 17:25:04 -0400 (EDT)
30, 29 -- Importance: Normal
30, 29 -- X-Priority: 3 (Normal)
30, 29 -- In-Reply-To: {200904242027.n3OKRNs3002810-at-ns.microscopy.com}
30, 29 -- References: {200904242027.n3OKRNs3002810-at-ns.microscopy.com}
30, 29 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
30, 29 -- MIME-Version: 1.0
30, 29 -- From: gas19-at-chrysler.com
30, 29 -- To: microscopy-at-microscopy.com
30, 29 -- X-MIMETrack: MIME-CD by Notes Server on sctcdm03.wk.dcx.com/Server/Prod/DCX(Release
30, 29 -- 8.0.2HF127 | October 9, 2008) at 04/24/2009 17:26:27,
30, 29 -- MIME-CD complete at 04/24/2009 17:26:27,
30, 29 -- Serialize by Router on sodddm23.wk.dcx.com/Server/Prod/DCX(Release
30, 29 -- 8.0.2HF887 | March 23, 2009) at 04/24/2009 17:25:06
30, 29 -- Message-ID: {OF5FFDAB41.E488DE31-ON852575A2.0075C789-852575A2.0075C78E-at-wkamerica.notes.chrysler.com}
30, 29 -- Date: Fri, 24 Apr 2009 17:26:28 -0400
30, 29 -- X-Mailer: Lotus Domino Web Server Release 8.0.2HF127 October 9, 2008
30, 29 -- Content-type: text/plain; charset=US-ASCII
30, 29 -- X-Brightmail-Tracker: AAAAAA==
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Sat, 25 Apr 2009 07:28:07 -0500
Subject: [Microscopy] Re: Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OLlgt2000484
for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 24 Apr 2009 16:47:42 -0500
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n3OLlgOh000482;
Fri, 24 Apr 2009 16:47:42 -0500

Randy,

A Sears Craftsman variable speed 3/8th inch drill works just as good, has
more low speed range, and takes any size carbon or graphite rod (1/8" to
1/4" or more). I made a sharpened rod one inch long that way with just a
Sears drill and a manual sharpener. I also used a fast turning lab scale
miniature lathe years ago but the drill works better and is smaller but
heavier than a Dremel tool. Kiss sore and black fingers goodbye!

Paul Beauregard

At 11:23 AM 4/24/09 -0500, TindallR-at-missouri.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
4, 29 -- From beaurega-at-westol.com Fri Apr 24 16:47:42 2009
4, 29 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OLlgED000478
4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 16:47:42 -0500
4, 29 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
4, 29 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n3OLkTJM023011
4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 17:46:30 -0400
4, 29 -- Received: (qmail 28889 invoked by uid 89); 24 Apr 2009 21:46:36 -0000
4, 29 -- Received: from pitts-69-72-22-246.dynamic-dialup.coretel.net (HELO running) (69.72.22.246)
4, 29 -- by mail.winbeam.com with SMTP; 24 Apr 2009 21:46:36 -0000
4, 29 -- Message-Id: {3.0.6.32.20090424184901.007f1da0-at-pop3.norton.antivirus}
4, 29 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified)
4, 29 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
4, 29 -- Date: Fri, 24 Apr 2009 18:49:01 -0500
4, 29 -- To: microscopy-at-microscopy.com
4, 29 -- From: Beaurega {beaurega-at-westol.com}
4, 29 -- Subject: Re: [Microscopy] Sharpening carbon rods without saying bad
4, 29 -- words
4, 29 -- In-Reply-To: {200904241623.n3OGNTjE022811-at-ns.microscopy.com}
4, 29 -- Mime-Version: 1.0
4, 29 -- Content-Type: text/plain; charset="us-ascii"
4, 29 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
4, 29 -- X-Winbeam-MailScanner-ID: n3OLkTJM023011
4, 29 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
4, 29 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted),
4, 29 -- SpamAssassin (not cached, score=-1.764, required 4,
4, 29 -- autolearn=not spam, AWL 0.16, BAYES_00 -2.00, TW_MX 0.08)
4, 29 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
4, 29 -- X-Winbeam-MailScanner-Watermark: 1241214391.11213-at-6vXOJrKU8khGXYlZFq3ZYg
==============================End of - Headers==============================

From katamail.com-select6-at-myway.com Sat Apr 25 00:30:56 2009
Return-Path: {katamail.com-select6-at-myway.com}
Received: from google.com ([84.36.74.94])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3P5UtnI003300
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 25 Apr 2009 00:30:55 -0500
Received: from [150.98.96.94] (HELO google.com)
by cozysock.us; Sat, 25 Apr 2009 07:35:25 +0200

These are two good refs for metals:

ASM Metals Handbook Volume 9 Metallography and Microstructures

Metallography Principles and Practice by George Vander Voort

Jeff Stewart
Materials Characterization Lab Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329

On Fri Apr 24 16:21 , oshel1pe-at-cmich.edu sent:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America




==============================Original Headers==============================
9, 32 -- From SRS0=f5cEeZ=AX=metallography.com=jeff-at-eigbox.net Sat Apr 25 07:28:07 2009
9, 32 -- Received: from bosmailout10.eigbox.net (bosmailout10.eigbox.net [66.96.186.10])
9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3PCS52a021925
9, 32 -- for {microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 07:28:06 -0500
9, 32 -- Received: from bosmailscan07.eigbox.net ([10.20.15.7])
9, 32 -- by bosmailout10.eigbox.net with esmtp (Exim)
9, 32 -- id 1LxgzD-0006BO-5G
9, 32 -- for microscopy-at-microscopy.com; Sat, 25 Apr 2009 08:28:03 -0400
9, 32 -- Received: from bosimpout01.eigbox.net ([10.20.55.1])
9, 32 -- by bosmailscan07.eigbox.net with esmtp (Exim)
9, 32 -- id 1LxgzC-0002cy-Sc
9, 32 -- for microscopy-at-microscopy.com; Sat, 25 Apr 2009 08:28:02 -0400
9, 32 -- Received: from boswebmail24.eigbox.net ([10.20.16.24])
9, 32 -- by bosimpout01.eigbox.net with NO UCE
9, 32 -- id jkPp1b0010X90oA0000000; Sat, 25 Apr 2009 04:23:49 -0400
9, 32 -- X-EN-OrigOutIP: 10.20.16.24
9, 32 -- X-EN-IMPSID: jkPp1b0010X90oA0000000
9, 32 -- Received: from [71.184.34.132] by boswebmail24.eigbox.net via HTTP; Sat, 25 Apr 2009 08:27:50 -0400
9, 32 -- Content-Disposition: inline
9, 32 -- Content-Type: text/plain; charset="iso-8859-1"
9, 32 -- MIME-Version: 1.0
9, 32 -- From: {jeff-at-metallography.com}
9, 32 -- To: Microscopy-at-microscopy.com
9, 32 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
9, 32 -- Reply-To: jeff-at-metallography.com
9, 32 -- X-Origin: 71.184.34.132
9, 32 -- Date: Sat, 25 Apr 2009 08:27:41 -0400
9, 32 -- Message-Id: {60203.1240662461-at-metallography.com}
9, 32 -- X-Mailer: AtMail 4.61 - 71.184.34.132 - jeff-at-metallography.com
9, 32 -- Sender: {jeff-at-metallography.com}
9, 32 -- Content-Transfer-Encoding: 8bit
9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3PCS52a021925
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Sat, 25 Apr 2009 14:25:52 -0500
Subject: [Microscopy] HT cable wanted for Zeiss EM10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Do not despair! Fine the organisation local to you that deals with x-ray
equipment in industry or hospitals. It is my experience that if you take
along the two unique ends of your old high voltage cable they will be able
to repair it for you. I did this many times in many different countries and
I often found they were so interested to work on a different type of
application that this was recognised in the basic fee that they charged!

For me it was always cheaper than obtaining a new cable from Japan, for you
it could resurrect your instrument?

Good luck.

Steve

Steve Chapman
Protrain
For training and consultancy in electron microscopy world wide
Tel +44 1280 816512 Fax +44 1280 814007
Cell +44 7711 606967 www.emcourses.com

-----Original Message-----
X-from: RCsencsits-at-lbl.gov [mailto:RCsencsits-at-lbl.gov]
Sent: 24 April 2009 21:26
To: protrain-at-emcourses.com

Our high voltage cable went bad on our little old Zeiss EM10, vintage
1976. Would anyone have a spare?
Can you recommend a company to make a new one?
Thanks,

Roseann Csencsits, PhD
Scientist in Charge - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548

==============================Original Headers==============================
2, 23 -- From RCsencsits-at-lbl.gov Fri Apr 24 15:25:11 2009
2, 23 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45])
2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n3OKPBAX028666
2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:25:11
-0500
2, 23 -- X-Ironport-SBRS: 2.3
2, 23 -- X-IronPort-Anti-Spam-Filtered: true
2, 23 -- X-IronPort-Anti-Spam-Result:
Aj4CAIO88UmAAykYe2dsb2JhbACWTgEBFiIFqUkJj3aCQYEzBQ
2, 23 -- X-IronPort-AV: E=Sophos;i="4.40,243,1239001200";
2, 23 -- d="scan'208";a="12586616"
2, 23 -- Received: from mta1.lbl.gov ([128.3.41.24])
2, 23 -- by ironport4.lbl.gov with ESMTP; 24 Apr 2009 13:25:10 -0700
2, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov
(apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246])
2, 23 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n3OKPADO025737
2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Apr 2009 13:25:10
-0700 (PDT)
2, 23 -- Message-Id: {EF4BAEF5-F5E7-49C1-B756-C1AB159127BF-at-lbl.gov}
2, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
2, 23 -- To: Microscopy-at-microscopy.com
2, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
2, 23 -- Content-Transfer-Encoding: 7bit
2, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
2, 23 -- Subject: HT cable wanted for Zeiss EM10
2, 23 -- Date: Fri, 24 Apr 2009 13:25:10 -0700
2, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================


==============================Original Headers==============================
14, 27 -- From protrain-at-emcourses.com Sat Apr 25 14:25:51 2009
14, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3PJPoPd024532
14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 14:25:51 -0500
14, 27 -- Received: from 5ad57292.bb.sky.com ([90.213.114.146]:2167 helo=HP6220)
14, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
14, 27 -- (envelope-from {protrain-at-emcourses.com} )
14, 27 -- id 1LxnVU-000738-2u by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Sat, 25 Apr 2009 21:25:48 +0200
14, 27 -- Reply-To: {protrain-at-emcourses.com}
14, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com}
14, 27 -- To: {RCsencsits-at-lbl.gov}
14, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com}
14, 27 -- References: {200904242026.n3OKQEH7031313-at-ns.microscopy.com}
14, 27 -- Subject: RE: [Microscopy] HT cable wanted for Zeiss EM10
14, 27 -- Date: Sat, 25 Apr 2009 20:25:26 +0100
14, 27 -- Organization: Protrain
14, 27 -- Message-ID: {014d01c9c5db$a53a4960$0200a8c0-at-HP6220}
14, 27 -- MIME-Version: 1.0
14, 27 -- Content-Type: text/plain;
14, 27 -- charset="us-ascii"
14, 27 -- Content-Transfer-Encoding: 7bit
14, 27 -- X-Mailer: Microsoft Office Outlook 11
14, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
14, 27 -- Thread-Index: AcnFGvb3MVBJ58D9To2D8eXyfobIVgAv4Tog
14, 27 -- In-Reply-To: {200904242026.n3OKQEH7031313-at-ns.microscopy.com}
14, 27 -- X-Scan-Signature: d8053df69de9c664bd038f8acb29a810{81}}
14, 27 -- X-Trace: smtp01.dial-up.net 1LxnVU-000738-2u dde184db835de61af203cf8137bad510
==============================End of - Headers==============================




From: rstallcup-at-zyvex.com
Date: Sat, 25 Apr 2009 22:13:23 -0500
Subject: [Microscopy] Basic Materials TEM/SEM/LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been following this tread and I wanted to reach out regarding the subject of materials.

I am an Graduate Artist at New York University doing research on art/science collaborations. Using the artistic medium to stimulate and educate the viewer about scientific fields that affect their everyday lives.
Clearly material science is one such field.
I am interested in large SEM fracture topology images of Aluminum alloy. I have been told that AL has a very interesting crystalline structure plus the use of AL in aircraft is a direct (and visceral) connection to the general public.
The end use of this image data would be constructing a large scale sculpture of the fracture topology. Vector (z-depth) data would be most helpful for construction, since I could port it into a 3-d software package (has anyone done this?), but I was informed that STM microscopy is not often used for fracture analysis.

I am sure all of you have seen amazing things on the micro-scale, Ideas and comments would be appreciated. I am looking for collaboration.



Cheers,

Brian Jones
ITP
721 Broadway, 4th Floor
New York, NY 10003
http://itp.nyu.edu

----- Original Message -----
X-from: jeff-at-metallography.com

Brian,

It looks like you are interested in materials and there is a lot of
fascinating topology. But have you considered microelectronics? I have
seen some amazing SEM images of VLSI MOSFET technology and we use this
technology in our everyday lives.

Richard

--
........................................................................
Richard E. Stallcup II, PhD
Applications Manager,
NanoWorks® Tools; Senior Scientist

Zyvex Instruments, LLC
Providing Nanotechnology Solutions – Today®

t: 972.792.1619
c: 972-522-9870
f: 972.235.7882
e: rstallcup-at-zyvex.com
w: www.zyvex.com
........................................................................
Notice of Confidentiality:

The information contained in this transmission
is privileged and confidential and is intended only
for the use of the addressee(s).

This e-mail is sent for business reasons only and
should be considered confidential.
........................................................................




==============================Original Headers==============================
10, 24 -- From rstallcup-at-zyvex.com Sat Apr 25 22:13:23 2009
10, 24 -- Received: from thebe.zyvex.com (gw.zyvex.com [24.173.8.226])
10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3Q3DNLj009114
10, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 22:13:23 -0500
10, 24 -- Received: from [192.168.1.100] (67-198-24-182.dyn.grandenetworks.net [67.198.24.182])
10, 24 -- (authenticated bits=0)
10, 24 -- by thebe.zyvex.com (8.13.1/8.13.1) with ESMTP id n3Q3DImf006520
10, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 22:13:19 -0500
10, 24 -- Message-ID: {49F3D14E.6040601-at-zyvex.com}
10, 24 -- Date: Sat, 25 Apr 2009 22:13:18 -0500
10, 24 -- From: Richard Stallcup {rstallcup-at-zyvex.com}
10, 24 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
10, 24 -- MIME-Version: 1.0
10, 24 -- To: Microscopy-at-microscopy.com
10, 24 -- Subject: [Microscopy] Basic Materials TEM/SEM/LM book ideas
10, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 24 -- Content-Transfer-Encoding: 8bit
10, 24 -- X-Spam-Status: No, score=0.1 required=5.0 tests=RDNS_DYNAMIC
10, 24 -- autolearn=disabled version=3.2.5
10, 24 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on thebe.zyvex.com
10, 24 -- X-Virus-Scanned: ClamAV 0.93.3/9288/Fri Apr 24 21:22:05 2009 on thebe.zyvex.com
10, 24 -- X-Virus-Status: Clean
10, 24 -- Received-SPF: pass (thebe.zyvex.com: 67.198.24.182 is authenticated by a trusted mechanism)
==============================End of - Headers==============================




From: mgengle-at-email.uky.edu
Date: Mon, 27 Apr 2009 08:17:43 -0500
Subject: [Microscopy] sections and grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers,
I noticed that after the advent of thin bar grids, sections didn't stick as well, probably because there was less surface area for sections to adhere to. Like Pat Common, I just put them in a 50 degree oven and never lose sections. 30 minutes works but we've left them in there inadvertently for several days, and they are fine. No pre-cleaning is necessary. An absolute ethanol dip does seem to minimize the tendency for sections to "run away" and also cuts down on what I call the jello water effect.

Mary Gail Engle
Sr Research Facility Manager
Electron Microscopy & Imaging Facility
HSRB rm 001
Ph (859) 323-6108
FAX (859) 323-8089
BBSRB rm o74
Ph (859)323-2701
FAX (859) 257-1581
University of KY
Lexington, KY 40536



==============================Original Headers==============================
3, 24 -- From mgengle-at-email.uky.edu Mon Apr 27 08:17:42 2009
3, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RDHd52029806
3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 08:17:41 -0500
3, 24 -- Received: from ex7hb04.ad.uky.edu ([128.163.187.54])
3, 24 -- by ironporta.uky.edu with ESMTP; 27 Apr 2009 09:17:35 -0400
3, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB04.ad.uky.edu
3, 24 -- ([128.163.187.54]) with mapi; Mon, 27 Apr 2009 09:17:36 -0400
3, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu}
3, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
3, 24 -- Date: Mon, 27 Apr 2009 09:17:34 -0400
3, 24 -- Subject: sections and grids
3, 24 -- Thread-Topic: sections and grids
3, 24 -- Thread-Index: AcnHOofDxkPdDT1mQVOpDKiqQQVpLw==
3, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A857095BBC1AA5-at-EX7FM03.ad.uky.edu}
3, 24 -- Accept-Language: en-US
3, 24 -- Content-Language: en-US
3, 24 -- X-MS-Has-Attach:
3, 24 -- X-MS-TNEF-Correlator:
3, 24 -- acceptlanguage: en-US
3, 24 -- Content-Type: text/plain; charset="us-ascii"
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Transfer-Encoding: 8bit
3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3RDHd52029806
==============================End of - Headers==============================




From: erin-at-aaisolutions.com
Date: Mon, 27 Apr 2009 17:16:43 -0500
Subject: [Microscopy] viaWWW: SmartScope Flare 200 needs new home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sousan.abolhassani-at-psi.ch as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sousan.abolhassani-at-psi.ch
Name: Sousan Abolhassani

Organization: Paul Scherrer Institut

Title-Subject: [Filtered] Interdisciplinary Symposium on 3D microscopy 2009

Question: Dear Colleagues,

The "Interdisciplinary Symposium on 3D microscopy
2009" will take place in Interlaken, Switzerland,
between 12th and 16th of July.

This meeting will be an exiting forum to meet
with scientists working in all fields of
microscopy using fascinating techniques to study
3 dimensional objects.
We would like to encourage you to participate to
this meeting and contribute to its success by
bringing new ideas and innovations that you are
using or plan to use in this field.

World renown speakers and experts will talk about
the state of the art in all different disciplines.

Suggested Topics:
High resolution TEM and AFM
3D CLSM and Light Microscopy
Stereology
3D TEM Tomography and Serial Sectioning
3D X-ray Microscopy and Tomography
3D FIB/SEM or Serial Sectioning
3D Image Analysis and simulation
Scanning Probe Microscopy

Plenary Lectures:
Free electron laser (XFEL);
Travelling-wave MRI and
Scanning Force Microscopy on Mars

Further interesting topics such as atom probe will also be treated.

Organizing committee and chairpersons are:
M. Cantoni, M. D¸rrenberger, C. Genoud, L.
Holzer, M. Ochs, M. Stampanoni, U. Staufer, R.
Wepf
and S. Abolhassani

I would like to invite you to visit the following
link to know more about this conference and
welcome you to participate to this event.

http://www.ssom.ch/3D/index.html

On behalf of the organizing committee,

Dr. Sousan Abolhassani
Paul Scherrer Institut
5232 Villigen-PSI
Switzerland

Login Host: 192.33.126.162
---------------------------------------------------------------------------


==============================Original Headers==============================
18, 13 -- From zaluzec-at-microscopy.com Mon Apr 27 08:21:47 2009
18, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
18, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RDLkx7030954
18, 13 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 08:21:47 -0500
18, 13 -- Mime-Version: 1.0
18, 13 -- Message-Id: {p06240801c61b61d4b6ef-at-[206.69.208.22]}
18, 13 -- Date: Mon, 27 Apr 2009 08:21:46 -0500
18, 13 -- To: microscopy-at-microscopy.com
18, 13 -- From: sousan.abolhassani-at-psi.ch (by way of MicroscopyListserver)
18, 13 -- Subject: viaWWW: Interdisciplinary Symposium on 3D microscopy 2009
18, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
18, 13 -- Content-Transfer-Encoding: 8bit
18, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3RDLkx7030954
==============================End of - Headers==============================

From tuckertwct-at-aol.com Mon Apr 27 09:59:16 2009
Return-Path: {tuckertwct-at-aol.com}
Received: from google.com (221-172.skknet.net [85.11.172.221] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RExDeS029669
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 27 Apr 2009 09:59:15 -0500
Received: from [141.81.20.83] (HELO google.com)
by thinyclammed.de; Mon, 27 Apr 2009 17:59:15 +0300
Message-ID: {000000031EFF2D9431498818}
Reply-To: Phemie Dull {meade.tarring15632-at-gmail.com}

AFM in Biology Class
Asylum Research, Santa Barbara, CA
June 3-5

This comprehensive class is open to all AFM scientists that wish to
expand their AFM knowledge as it pertains to life science applications.
The class includes both lecture and extensive hands-on experiments with
topics on:

• Basic AFM operation (as demonstrated on the MFP-3D AFM)
• Biological sample preparation and interpretation of AFM data
• Choice of cantilevers
• Imaging samples in air and fluids: from molecules to cells
• Force measurements: intra molecular forces and hardness measurements
• Simultaneous AFM and optical microscopy techniques including
fluorescence and phase contrast
• Recognizing artifacts

Additional information can be found at www.asylumresearch.com.

Terry Mehr
Asylum Research


==============================Original Headers==============================
6, 13 -- From terry-at-AsylumResearch.com Mon Apr 27 12:18:34 2009
6, 13 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129])
6, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RHIXj5004087
6, 13 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 12:18:33 -0500
6, 13 -- Date: Mon, 27 Apr 2009 10:18:30 -0700
6, 13 -- From: Terry Mehr {terry-at-AsylumResearch.com}
6, 13 -- User-Agent: Thunderbird 2.0.0.6 (Macintosh/20070728)
6, 13 -- MIME-Version: 1.0
6, 13 -- To: Microscopy-at-microscopy.com
6, 13 -- Subject: AFM in Biology Class June 3-5, 2009
6, 13 -- Content-Type: text/plain; charset=windows-1252; format=flowed
6, 13 -- Content-Transfer-Encoding: 8bit
6, 13 -- Message-ID: {1LyUTP-0000Ik-7Z-at-exchange.AsylumResearch.com}
==============================End of - Headers==============================

From whdvch-at-hotmail.com Mon Apr 27 14:30:04 2009
Return-Path: {whdvch-at-hotmail.com}
Received: from google.com (88-109-253-202.dynamic.dsl.as9105.com [88.109.253.202])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RJU2pi025127
for {microscopylistserverarchive-at-microscopy.com} ; Mon, 27 Apr 2009 14:30:03 -0500
Received: from [187.114.40.181] (HELO google.com)
by spottedmill.net; Mon, 27 Apr 2009 20:30:04 +0100
Message-ID: {00000002ED826B3869110148}
Reply-To: Mordecai Wiggins {18553weston.hillerud-at-gmail.com}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both erin-at-aaisolutions.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: erin-at-aaisolutions.com
Name: Erin Curry

Organization: AAI

Title-Subject: [Filtered] SmartScope Flare 200 needs new home

Question: Hello,
We have an Optical Gaging Smartscope Flare 200 video metrology system
available, complete with PC, software, and antivibration platform.
Please contact erin-at-aaisolutions.com for photos and additional
details.
Thanks,
Erin

Login Host: 64.81.17.181
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Apr 27 17:16:43 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RMGggb032356
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 17:16:42 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c61bdf351a22-at-[206.69.208.22]}
6, 11 -- Date: Mon, 27 Apr 2009 17:16:41 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: erin-at-aaisolutions.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: SmartScope Flare 200 needs new home
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rnichols-at-bcm.edu
Date: Mon, 27 Apr 2009 17:17:14 -0500
Subject: [Microscopy] viaWWW: GMA (glycomethacrylate) blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rnichols-at-bcm.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rnichols-at-bcm.edu
Name: ralph nichols

Organization: Baylor College of Medicine

Title-Subject: [Filtered] GMA

Question: Hello Listers

I have project that involve GMA (glycomethacrylate) blocks.
The investigator wants to do TEM on blocks after light
microscopy has been done. My question is there any
procedures to remove GMA from tissue to do TEM.

Thanks

Ralph Nichols
Baylor College of Medicine
Houston TX


Login Host: 128.249.96.252
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Apr 27 17:17:14 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RMHCrN032708
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 17:17:14 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c61bdf53212b-at-[206.69.208.22]}
10, 11 -- Date: Mon, 27 Apr 2009 17:17:12 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: rnichols-at-bcm.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: GMA (glycomethacrylate) blocks
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Tue, 28 Apr 2009 03:00:06 -0500
Subject: [Microscopy] Re: GMA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,
dear Ralph,
dear all,

It was my understanding of using / having once or twice used GMA (glycol-methacrylate resin i.e. as Technovit 7100) a long time ago that either LM as well as EM can/could be done on the same block without attempting or necessity/possibility to remove the resin first for ultrathin sectioning. Such a task implies to use another formulation ("hard")from the initial stage rather than a resin mixing formula used only for LM-application(s).
Removal of resin seems to be / is impossible
( cf. e.g. Histonet-communication and other sources on that subject
http://www.histosearch.com/histonet/Feb06/RE.HistonetTechnovit7100rB.html )

GMA in some way behaves differently from Epoxy-type resins (in terms of hardness/brittleness, section properties etc., etc.) but as far as to my knowledge it would be possible to apply directly "histological-histochemical stainings" as well as EM-heavy metal contrast /staining solutions on to ultrathin sections.

Perhaps it will be of help to visit a website at SPI which seems to contain more specified if not comprehensive information about the stuff you should work with (if you do not have access to the original "old" literature/papers):

http://www.2spi.com/catalog/chem/low-acid-gma-instructions.html

Also, you could search in the URLs-Index of
http://www.ebsciences.com/papers/index.html :

Initial statement there: NOTE: GMA resin-based products (Technovit® 7100, 8100) should not be used when epoxy removal from specimens is required. There is no known method of GMA epoxy removal (including methoxide) that does not effectively render specimens unusable. Instead, MMA resin-based products such as Technovit® 9100 should be selected

cf. also Section: Glycol Methacrylate: Embedding and Staining
Technovit® 7100 and 8100 Application Information from Heraeus Kulzer Embedding Protocols
and
Methyl Methacrylate: Embedding and Staining
Technovit® 9100 Application Information from Heraeus Kulzer
and microtomy -at- http://www.ebsciences.com/histology/methacrylate.htm#5



Usual Disclaimer applies for companys mentioned in URL's....no personal affiliation, no interest...

The real question for me is left unanswered: why it is necessary for you/your client to remove GMA resin for TEM sectioning/staining at all ?

Best wishes and good luck,

Wolfgang Muß
Salzburg-Austria



This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal

===========================================
OR Dr. phil. Wolfgang Muss
Head of EM-Lab
Institute of Pathology, SALK-LKH
(Salzburger Landeskliniken gemeinnuetzige GesmbH, Landeskrankenhaus = Fed. State Gen. Hosp.)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively (same Lab, same address)

Paracelsus Medical Private University (PMU)
Univ.-Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work: +43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss")
E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {

-------------------------------------------------------------------------
Forthcoming in 2009:
+++2009, June 11th - June 13th: 36th Ann. SCUR Meeting (SCUR meets Florence),
Host: Francesca Prignano and her team+++
We cordially invite you to participate actively in the meeting.
Visit: Next Meetings at:
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/index_ger.html








} -----Ursprüngliche Nachricht-----
} Von: rnichols-at-bcm.edu [mailto:rnichols-at-bcm.edu]
} Gesendet: Dienstag, 28. April 2009 00:23
} An: Muß Wolfgang
} Betreff: [Microscopy] GMA (glycolmethacrylate) blocks for LM-followed by TEM? (removal of GMA?)

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both rnichols-at-bcm.edu as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
} Email: rnichols-at-bcm.edu
} Name: ralph nichols
} Organization: Baylor College of Medicine
} Title-Subject: GMA
} Question:
}
}
}
} Hello Listers
}
} I have project that involve GMA (glycomethacrylate) blocks.
}
} The investigator wants to do TEM on blocks after light
} microscopy has been done. My question is there any
} procedures to remove GMA from tissue to do TEM.
}
} Thanks
}
} Ralph Nichols
} Baylor College of Medicine
} Houston TX
}
}
} Login Host: 128.249.96.252
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Mon Apr 27 17:17:14 2009
} 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n3RMHCrN032708
} 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr
} 2009 17:17:14 -0500
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240802c61bdf53212b-at-[206.69.208.22]}
} 10, 11 -- Date: Mon, 27 Apr 2009 17:17:12 -0500
} 10, 11 -- To: microscopy-at-microscopy.com
} 10, 11 -- From: rnichols-at-bcm.edu (by way of MicroscopyListserver)
} 10, 11 -- Subject: viaWWW: GMA (glycomethacrylate) blocks
} 10, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
33, 36 -- From W.Muss-at-salk.at Tue Apr 28 03:00:06 2009
33, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
33, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3S804Ss015022
33, 36 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 03:00:05 -0500
33, 36 -- Received: from localhost (localhost [127.0.0.1])
33, 36 -- by hermes.salk.at (Postfix) with ESMTP id 3CB8CC385F;
33, 36 -- Tue, 28 Apr 2009 10:00:02 +0200 (CEST)
33, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
33, 36 -- Received: from hermes.salk.at ([127.0.0.1])
33, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
33, 36 -- with ESMTP id jzcXWAeQ+x35; Tue, 28 Apr 2009 10:00:01 +0200 (CEST)
33, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
33, 36 -- by hermes.salk.at (Postfix) with ESMTP id C36EDC3850;
33, 36 -- Tue, 28 Apr 2009 10:00:01 +0200 (CEST)
33, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
33, 36 -- Tue, 28 Apr 2009 10:00:01 +0200
33, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
33, 36 -- Content-class: urn:content-classes:message
33, 36 -- MIME-Version: 1.0
33, 36 -- Content-Type: text/plain;
33, 36 -- charset="iso-8859-1"
33, 36 -- Subject: [Microscopy] Re: GMA
33, 36 -- Date: Tue, 28 Apr 2009 10:00:01 +0200
33, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D09D9-at-N1RZ116.lksdom21.lks.local}
33, 36 -- In-Reply-To: {200904272223.n3RMNT1U011115-at-ns.microscopy.com}
33, 36 -- X-MS-Has-Attach:
33, 36 -- X-MS-TNEF-Correlator:
33, 36 -- Thread-Topic: [Microscopy] Re: GMA
33, 36 -- Thread-Index: AcnHhs8iJZp1wjPrRXS6LS98icg1uAAR3vxw
33, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
33, 36 -- To: {microscopy-at-microscopy.com}
33, 36 -- Cc: {rnichols-at-bcm.edu}
33, 36 -- X-OriginalArrivalTime: 28 Apr 2009 08:00:01.0822 (UTC) FILETIME=[553543E0:01C9C7D7]
33, 36 -- X-Scanned-By: SALK-Content-Filter
33, 36 -- Content-Transfer-Encoding: 8bit
33, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3S804Ss015022
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Tue, 28 Apr 2009 09:17:40 -0500
Subject: [Microscopy] Microscopy Today May 2009 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the May 2009 Microscopy Today table of contents. We will
close the subscription list for this issue on Wednesday, April 1, 2009. Sorry for the short time interval as we are trying to beat the May 11th postal rate increase.
Microscopists in North America and MSA members anywhere qualify for free
subscriptions. All subscriptions at
http://www.microscopy-today.com .

The July issue of MT will be totally reformatted with a new look and new features under Charles Lyman, the new editor. Not least of which will be an exact duplicate digital edition available nearly simultaneously. All URLs and emails in the digital edition will be live so that you may go to the desired link from within the digital edition.

Thank you,
Ron Anderson, Managing Editor

========================


Developmental Dynamics in Real Time
Stephen W. Carmichael, Mayo Clinic

Complexions: A Revolutionary Taxonomy for Grain Boundaries
Alwyn Eades, Lehigh University, Bethlehem, PA

Development of a 200kV Atomic Resolution Analytical Electron Microscope
T. Isabell*, et al., and I. Ishikawa**, et al. *JEOL USA, Inc., Peabody,
MA **JEOL Ltd., Akishima, Japan

Vibration Isolation Critical to Measuring Neuronal Patterns in the Brain
David L. Platus, Minus K Technology, Inc., Inglewood, CA

Phase Identification and Mapping Based on Valence Loss EELS and ELNES
R.D. Twesten, Gatan, Inc., Pleasanton, CA

Improved Techniques For Imaging Of Three-Dimensional Transparent
Specimens In Advanced Darkfield And Interference Contrast Modes
Jörg Piper, Clinic Meduna, Bad Bertrich, Germany

Low Energy, Low Angle, Large Area Ion Polishing for Improved EBSD Indexing
S.D. Walck*, J.R. Porter**, H-W. Yang**, S.S. Dheda**, *South Bay
Technology, Inc., San Clemente, CA, **UC Irvine, CA

Toward Robust High Resolution Chemical Imaging
C. A. Barrios, A. V. Malkovskiy, A. Kisliuk, A. P. Sokolov, M. D.
Foster, Dept. of Polymer Science, The U. of Akron, Akron, OH

Distinguishing the Data from the Dark: Single Source Software or
Microscopy Mix and Match?
Tim Oliver, Duke University Medical Center, Durham NC

Pioneers in Optics: Alhazen and Roger Bacon
Michael W. Davidson, National High Magnetic Field Laboratory, The
Florida State University, Tallahassee, FL

The Electron Gun its Saturation and Alignment—An Old Man’s Saga
Steve Chapman, Protrain, Buckingham, England

Determining the Micron Marker Distance or Magnification of a Microscopic
Image
Paul Beauregard, Chemist and Electron Microscopist, Greensburg, PA

A Very Simple Method for Quickly Making Large Numbers of Measurements on
Micrographs
Ron Anderson, Microscopy Today, Largo, FL

Dear Abbe

Industry News

NetNotes
SPECIMEN PREPARATION – waxy cuticles
SPECIMEN PREPARATION – TEM of fossil tooth
SPECIMEN PREPARATION – retinas
SPECIMEN PREPARATION - SEM of fibrin clots
SPECIMEN PREPARATION - liposomes
TEM - Oval beam
SEM – oil shale samples
SEM- magnetic materials
SEM – catholuminescence
EDX – broken detector window
EDX- mothball liquid nitrogen chilled detector
Convergent-beam electron diffraction – thickness measurement
Advertiser's Index


==============================Original Headers==============================
23, 17 -- From randerson20-at-tampabay.rr.com Tue Apr 28 09:17:40 2009
23, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
23, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3SEHdnS028510
23, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Apr 2009 09:17:40 -0500
23, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
23, 17 -- by hrndva-omta01.mail.rr.com with ESMTP
23, 17 -- id {20090428141739525.OTXR11305-at-hrndva-omta01.mail.rr.com}
23, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Apr 2009 14:17:39 +0000
23, 17 -- Message-ID: {49F70FFB.3030407-at-tampabay.rr.com}
23, 17 -- Date: Tue, 28 Apr 2009 10:17:31 -0400
23, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
23, 17 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
23, 17 -- MIME-Version: 1.0
23, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
23, 17 -- Subject: Microscopy Today May 2009 Table of Contents
23, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed
23, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: godfreew-at-evms.edu
Date: Tue, 28 Apr 2009 21:52:08 -0500
Subject: [Microscopy] viaWWW: Scanning electron microscope available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both godfreew-at-evms.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: godfreew-at-evms.edu
Name: Earl Godfrey, PhD

Organization: Eastern Virginia Medical School

Title-Subject: [Filtered] Scanning electron microscope

Question: Eastern Virginia Medical School owns a Phillips SEM515
scanning electron microscope, with Polaroid model 545 camera and EDAC
X-ray dispersive analysis unit attached. It is in the core facility
that I direct. It is not currently functioning, but needs about
$5000 in repairs. The school would like to give it away, preferably
to a non-profit organization.

Login Host: 157.21.25.109
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Apr 28 21:52:08 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3T2q7Sp023125
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 21:52:08 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c61d713854dd-at-[206.69.208.22]}
6, 11 -- Date: Tue, 28 Apr 2009 21:52:06 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: godfreew-at-evms.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Scanning electron microscope available
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vwporsche-at-verizon.net
Date: Tue, 28 Apr 2009 21:52:52 -0500
Subject: [Microscopy] viaWWW: Documentation on JEOL SEM 6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both vwporsche-at-verizon.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: vwporsche-at-verizon.net
Name: Rod Rowland

Organization: MATSYS

Title-Subject: [Filtered] Looking for Literature JEOL SEM 6100

Question: I just acquired a used JEOL SEM model 6100.
I did not receive any literature with this SEM and
would like to know if anyone can help me find some basic
user / setup info such as; operator manual, schematics, etc..?

Login Host: 69.244.232.212
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Apr 28 21:52:52 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3T2qpLh023836
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 21:52:52 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c61d71675fc7-at-[206.69.208.22]}
6, 11 -- Date: Tue, 28 Apr 2009 21:52:50 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: vwporsche-at-verizon.net (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Documentation on JEOL SEM 6100
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 28 Apr 2009 22:05:19 -0500
Subject: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, now you know the principle of through
fact finding before purchase or transfer of
a SEM. A "good deal" is not necessarily so.

Just advise to others--check the details.
If you do not know the details, do your
homework. An initial "good deal" could easily
become a nightmare afterwards. Ask the list.
There is much history out here. It is a rich
resource. Personal stuff can be easily off-line.

gary g.


At 07:54 PM 4/28/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Tue Apr 28 22:05:18 2009
9, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3T35ITv018776
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 22:05:18 -0500
9, 20 -- Message-Id: {200904290305.n3T35ITv018776-at-ns.microscopy.com}
9, 20 -- Received: (qmail 9631 invoked from network); 28 Apr 2009 20:17:13 -0700
9, 20 -- Received: by simscan 1.1.0 ppid: 9628, pid: 9629, t: 0.1062s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp1 with SMTP; 28 Apr 2009 20:17:13 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Tue, 28 Apr 2009 20:05:16 -0700
9, 20 -- To: vwporsche-at-verizon.net
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: Documentation on JEOL SEM 6100
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200904290254.n3T2sFNF027867-at-ns.microscopy.com}
9, 20 -- References: {200904290254.n3T2sFNF027867-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 29 Apr 2009 08:44:02 -0500
Subject: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
After first doing a more THOROUGH job spell-checking, the hassle (cost) of
getting replacement documentation might not be too great compared to a good
price on a used SEM. I suspect that Rod won't have too much trouble getting
documentation (although I can't help him out with a 6100) and he didn't
state what his costs were.

Just my $.02

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Tuesday, April 28, 2009 11:07 PM
To: kenconverse-at-qualityimages.biz

Well, now you know the principle of through
fact finding before purchase or transfer of
a SEM. A "good deal" is not necessarily so.

Just advise to others--check the details.
If you do not know the details, do your
homework. An initial "good deal" could easily
become a nightmare afterwards. Ask the list.
There is much history out here. It is a rich
resource. Personal stuff can be easily off-line.

gary g.


At 07:54 PM 4/28/2009, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Tue Apr 28 22:05:18 2009
9, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n3T35ITv018776
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 22:05:18
-0500
9, 20 -- Message-Id: {200904290305.n3T35ITv018776-at-ns.microscopy.com}
9, 20 -- Received: (qmail 9631 invoked from network); 28 Apr 2009 20:17:13
-0700
9, 20 -- Received: by simscan 1.1.0 ppid: 9628, pid: 9629, t: 0.1062s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp1 with SMTP; 28 Apr 2009 20:17:13 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Tue, 28 Apr 2009 20:05:16 -0700
9, 20 -- To: vwporsche-at-verizon.net
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: Documentation on JEOL SEM 6100
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200904290254.n3T2sFNF027867-at-ns.microscopy.com}
9, 20 -- References: {200904290254.n3T2sFNF027867-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




==============================Original Headers==============================
22, 25 -- From kenconverse-at-qualityimages.biz Wed Apr 29 08:43:59 2009
22, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3TDhvdL028810
22, 25 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 08:43:58 -0500
22, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
22, 25 -- with ESMTP
22, 25 -- id {20090429134355144.VDZN22077-at-cdptpa-omta02.mail.rr.com} ;
22, 25 -- Wed, 29 Apr 2009 13:43:55 +0000
22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
22, 25 -- To: {gary-at-gaugler.com} , "MSA Listserver" {microscopy-at-microscopy.com}
22, 25 -- Subject: RE: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100
22, 25 -- Date: Wed, 29 Apr 2009 09:43:45 -0400
22, 25 -- Message-ID: {238FFDA25F4443849159E381B2CEEDD2-at-Ken}
22, 25 -- MIME-Version: 1.0
22, 25 -- Content-Type: text/plain;
22, 25 -- charset="us-ascii"
22, 25 -- X-Priority: 3 (Normal)
22, 25 -- X-MSMail-Priority: Normal
22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
22, 25 -- Importance: Normal
22, 25 -- Thread-Index: AcnId6O1n1EXRGQbSV+rflmxFdLLAwAV/Aog
22, 25 -- In-Reply-To: {200904290307.n3T37Lef022759-at-ns.microscopy.com}
22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
22, 25 -- Content-Transfer-Encoding: 8bit
22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3TDhvdL028810
==============================End of - Headers==============================




From: ehuber-at-nature.berkeley.edu
Date: Wed, 29 Apr 2009 16:36:29 -0500
Subject: [Microscopy] Need advice on consumer digital camera & Image Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I am interested in taking photographs of fish scales and otoliths for
age and growth analyses at anywhere between 12.5X to 100X with a Leica
DM2000 transmitted light compound microscope. I was hoping to receive
advice about the best digital camera and coupler (preferably for $500 or
less with an emphasis on the 'less') to purchase for this purpose. I
intend to use Image Tool software for the analyses. Any advice
concerning use of this program, especially as it pertains to scale &
otolith analyses, would also be welcomed.

Thank you very much,
Eric

--
Eric R. Huber
Carlson Laboratory Manager
University of California, Berkeley
Dept. of Environmental Science, Policy & Management
140 Mulford Hall #3114
Berkeley, CA 94720
510-643-9688 (office)
508-446-5433 (cell)
510-643-5438 (fax)
Office: 304 Mulford Hall
http://nature.berkeley.edu/carlsonlab/
http://nature.berkeley.edu/~ehuber/


==============================Original Headers==============================
4, 27 -- From ehuber-at-nature.berkeley.edu Wed Apr 29 16:36:29 2009
4, 27 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [169.229.201.201])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3TLaSha003453
4, 27 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 16:36:28 -0500
4, 27 -- Received: from localhost (localhost [127.0.0.1])
4, 27 -- by nature.Berkeley.EDU (Postfix) with ESMTP id 99C9A364A7
4, 27 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 14:36:28 -0700 (PDT)
4, 27 -- Received: from nature.Berkeley.EDU ([127.0.0.1])
4, 27 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-maia, port 10024)
4, 27 -- with ESMTP id 00152-04-3 for {microscopy-at-microscopy.com} ;
4, 27 -- Wed, 29 Apr 2009 14:36:26 -0700 (PDT)
4, 27 -- Received: from [192.168.0.198] (mul-334a-304-007-d.ESPM.Berkeley.EDU [128.32.224.96])
4, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
4, 27 -- (No client certificate requested)
4, 27 -- (Authenticated sender: ehuber-at-nature)
4, 27 -- by nature.Berkeley.EDU (Postfix) with ESMTPSA id A469F36466
4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 29 Apr 2009 14:36:26 -0700 (PDT)
4, 27 -- Message-ID: {49F8C85C.5060008-at-nature.berkeley.edu}
4, 27 -- Date: Wed, 29 Apr 2009 14:36:28 -0700
4, 27 -- From: Eric Huber {ehuber-at-nature.berkeley.edu}
4, 27 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
4, 27 -- MIME-Version: 1.0
4, 27 -- To: Microscopy-at-microscopy.com
4, 27 -- Subject: Need advice on consumer digital camera & Image Tool
4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 27 -- Content-Transfer-Encoding: 7bit
4, 27 -- X-Virus-Scanned: Maia Mailguard 1.0.2
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 29 Apr 2009 23:30:45 -0500
Subject: [Microscopy] viaWWW: Documentation on JEOL SEM 6100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, Ken.

Are you saying that my feedback is faulty because of
spelling? Perfeck spelling was not my intent. The
issue is getting all the docs with a "new" system.

For example, Zeiss will not provide schematics. They
will provide maintenance docs, but these are bound to
the requestor...they are not transferable. And they have
some limit of useability.

If I were to quibble about all the spelling errors on
postings, I would be overloaded. So, what is your point?
Bad spelling means bad input? How can this be proven?
What is your point?

garrry g.


At 06:43 AM 4/29/2009, you wrote:
} Gary,
} After first doing a more THOROUGH job spell-checking, the hassle (cost) of
} getting replacement documentation might not be too great compared to a good
} price on a used SEM. I suspect that Rod won't have too much trouble getting
} documentation (although I can't help him out with a 6100) and he didn't
} state what his costs were.
}
} Just my $.02
}
} Ken Converse
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} From: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
} Sent: Tuesday, April 28, 2009 11:07 PM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 21 -- From gary-at-gaugler.com Wed Apr 29 23:30:45 2009
8, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3U4UiIb001902
8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 23:30:45 -0500
8, 21 -- Message-Id: {200904300430.n3U4UiIb001902-at-ns.microscopy.com}
8, 21 -- Received: (qmail 19643 invoked from network); 29 Apr 2009 21:25:46 -0700
8, 21 -- Received: by simscan 1.1.0 ppid: 19640, pid: 19641, t: 0.1492s
8, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
8, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
8, 21 -- by smtp2 with SMTP; 29 Apr 2009 21:25:46 -0700
8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 21 -- Date: Wed, 29 Apr 2009 21:30:42 -0700
8, 21 -- To: "Ken Converse" {kenconverse-at-qualityimages.biz}
8, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
8, 21 -- Subject: RE: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100
8, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
8, 21 -- In-Reply-To: {238FFDA25F4443849159E381B2CEEDD2-at-Ken}
8, 21 -- References: {200904290307.n3T37Lef022759-at-ns.microscopy.com}
8, 21 -- {238FFDA25F4443849159E381B2CEEDD2-at-Ken}
8, 21 -- Mime-Version: 1.0
8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: StevenLe-at-BaylorHealth.edu
Date: Thu, 30 Apr 2009 07:52:59 -0500
Subject: [Microscopy] 3rd Party TEM Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anyone recommend a reliable 3rd party service company for a LEO906E TEM, that services the Dallas/Ft. Worth area.

Thanks,
Steve




**********************************************************************
This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy-at-baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.


==============================Original Headers==============================
4, 28 -- From StevenLe-at-BaylorHealth.edu Thu Apr 30 07:52:58 2009
4, 28 -- Received: from bhdappagnt02.baylorhealth.edu (mailhost1.baylorhealth.edu [65.248.93.160])
4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3UCqweD006313
4, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 07:52:58 -0500
4, 28 -- Received: from BHDAEXIMS02.bhcs.pvt ([10.100.14.73])
4, 28 -- by bhdappagnt02.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id n3UCqwXK029668
4, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 07:52:58 -0500
4, 28 -- X-TM-IMSS-Message-ID: {330781c00006db01-at-bhcs.pvt}
4, 28 -- Received: from BHDAEXHT02.bhcs.pvt ([10.5.12.191]) by bhcs.pvt ([10.100.14.73]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 330781c00006db01 ; Thu, 30 Apr 2009 07:52:57 -0600
4, 28 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT02.bhcs.pvt
4, 28 -- ([10.5.12.191]) with mapi; Thu, 30 Apr 2009 07:52:57 -0500
4, 28 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu}
4, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
4, 28 -- Date: Thu, 30 Apr 2009 07:52:56 -0500
4, 28 -- Subject: 3rd Party TEM Service
4, 28 -- Thread-Topic: 3rd Party TEM Service
4, 28 -- Thread-Index: AcnJkpVygTROcHn6RB69vtO4cOuIqA==
4, 28 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0CDD80CAAA-at-BHDAEXVM32.bhcs.pvt}
4, 28 -- Accept-Language: en-US
4, 28 -- Content-Language: en-US
4, 28 -- X-MS-Has-Attach:
4, 28 -- X-MS-TNEF-Correlator:
4, 28 -- acceptlanguage: en-US
4, 28 -- Content-Type: text/plain; charset="us-ascii"
4, 28 -- MIME-Version: 1.0
4, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-04-30_07:2009-04-28,2009-04-30,2009-04-29 signatures=0
4, 28 -- Content-Transfer-Encoding: 8bit
4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3UCqweD006313
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Thu, 30 Apr 2009 08:33:32 -0500
Subject: [Microscopy] viaWWW: TEM: Magnetic Field Cancellation Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: David Mitchell

Organization: EM Unit, University of Sydney

Title-Subject: [Filtered] TEM: Magnetic Field Cancellation Systems

Question: Dear All

We are currently commissioning a new 200kV TEM/STEM with an in-column
filter. The room only just met environmental specs with respect to
magnetic field levels, and the new instrument does meets performance
specs in terms of STEM resolution. However, I think it could be
better. A fixed probe at 1 million times does show evidence of
external magnetic field interference. These is some jitter in the
1-10Hz range of about 1nm amplitude, and there is much lower
frequency drift over longer time frames of } 1 minute. We have
measured AC fields in various planes, at typically 1mG or less, but
up to 2.2mG in some instances. I have used a Lundgren field
compensation system in a lab which was way out of spec (15mG), and so
realise that they can make a huge difference in those situations.
However, does anyone have experience with such systems in relatively
low field environments such as I have? Is any potential improvement
in STEM performance going to be worth the ca $50k investment?

Thanks in advance,

Dave Mitchell

Login Host: 129.78.64.103
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Apr 30 08:33:32 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3UDXV58022708
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 08:33:32 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c61f58f9a856-at-[206.69.208.22]}
9, 11 -- Date: Thu, 30 Apr 2009 08:33:30 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: TEM: Magnetic Field Cancellation Systems
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Thu, 30 Apr 2009 15:14:50 -0500
Subject: [Microscopy] Lab scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is the Epson v750 pro still the best sub-$1000 scanner available for
scanning EM negatives, blots, the occasional leaf or other flat
specimen, etc.?

Just thought I'd check before I pulled the trigger on this thing.

Thanks,

Andy Bowling


==============================Original Headers==============================
5, 31 -- From AJBowling-at-dow.com Thu Apr 30 15:14:49 2009
5, 31 -- Received: from mail145.messagelabs.com (mail145.messagelabs.com [216.82.242.163])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3UKEn2A027389
5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 15:14:49 -0500
5, 31 -- X-VirusChecked: Checked
5, 31 -- X-Env-Sender: AJBowling-at-dow.com
5, 31 -- X-Msg-Ref: server-13.tower-145.messagelabs.com!1241122484!7521101!7
5, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,-
5, 31 -- X-Originating-IP: [216.99.65.22]
5, 31 -- Received: (qmail 22637 invoked from network); 30 Apr 2009 20:14:48 -0000
5, 31 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22)
5, 31 -- by server-13.tower-145.messagelabs.com with RC4-SHA encrypted SMTP; 30 Apr 2009 20:14:48 -0000
5, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.3959);
5, 31 -- Thu, 30 Apr 2009 16:14:43 -0400
5, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 31 -- Content-class: urn:content-classes:message
5, 31 -- MIME-Version: 1.0
5, 31 -- Content-Type: text/plain;
5, 31 -- charset="US-ASCII"
5, 31 -- Subject: Lab scanner
5, 31 -- Date: Thu, 30 Apr 2009 16:14:41 -0400
5, 31 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1F60F14-at-USMDLMDOWX032.dow.com}
5, 31 -- X-MS-Has-Attach:
5, 31 -- X-MS-TNEF-Correlator:
5, 31 -- Thread-Topic: Lab scanner
5, 31 -- Thread-Index: AcnJ0ARGEp2LpXxrS7iDvDExF1H/6wAACSYw
5, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
5, 31 -- To: {microscopy-at-microscopy.com}
5, 31 -- X-OriginalArrivalTime: 30 Apr 2009 20:14:43.0285 (UTC) FILETIME=[4CA23850:01C9C9D0]
5, 31 -- Content-Transfer-Encoding: 8bit
5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3UKEn2A027389
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 30 Apr 2009 17:22:16 -0500
Subject: [Microscopy] Processing Fat for Paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello out there in fat processing land,

I have been given human fat samples and need to embed them in paraffin. In the past I've used a VIP processor for this and now I have an Autotechnicon (vintage dual model) with a timing wheel.

I know I need to process these fatties slowly, my question is--can I use 2 hours per step and have it turn out OK? I have a timing wheel punched out for 2 hour steps.

My steps would be alcohols: 70, 80, 95 x 2, 100 x 3, Citrisolv x3, paraffin x2 and another paraffin step under vacuum.

Let me know your wise and experienced opinions or protocols. I don't have anything else to use except the 43 year old Autotechnicon so don't even suggest it. You make me feel bad that I can't get my research foundation to buy me something new or even newer. ;-)

Stewing in somebody else's fat (eew!),

Paula :-)


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397





==============================Original Headers==============================
12, 26 -- From vapatpxs-at-yahoo.com Thu Apr 30 17:22:13 2009
12, 26 -- Received: from n70.bullet.mail.sp1.yahoo.com (n70.bullet.mail.sp1.yahoo.com [98.136.44.38])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3UMMAK4018582
12, 26 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 17:22:11 -0500
12, 26 -- Received: from [69.147.84.144] by n70.bullet.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000
12, 26 -- Received: from [69.147.84.114] by t6.bullet.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000
12, 26 -- Received: from [127.0.0.1] by omp203.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000
12, 26 -- X-Yahoo-Newman-Property: ymail-3
12, 26 -- X-Yahoo-Newman-Id: 601078.46609.bm-at-omp203.mail.sp1.yahoo.com
12, 26 -- Received: (qmail 64809 invoked by uid 60001); 30 Apr 2009 22:22:08 -0000
12, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1241130128; bh=ohjqwVa2mO8sAN+4pKRYxvmo3Luj25r6Dq2LNtxTXyg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=pBxxnkyEjuT4zjWH9bjjkeHcLjnavNOTOIH7jkK1NrYyVRvOW31rCrF+mCKsB07WBCOq6g0bIHvRyVVka4+QLSsOBaPEgVFZo7lSO+2AV3RhyuxTJ2YLkALJJSkTRACyTMVdQABgkudo5mZyTA+AGRY41bkz60HACZJtkQkTKiQ=
12, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 26 -- s=s1024; d=yahoo.com;
12, 26 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 26 -- b=hUH+Hn7j8VVbcVgY87bx9qhOR4YatWOaquwmOv4ZsQySssQ8bKVDu+zySPbeGHHb8teSF1bwUFvED9o8PFEJzBTngkEmrBC3+AVdnZ10YVAL1dBabPPBLFdeZAE0r32XwAqGkRb8WwSmkFbVzzfu/0VHjSQLHI4U0bj2Zfu0DsU=;
12, 26 -- Message-ID: {490013.63581.qm-at-web46103.mail.sp1.yahoo.com}
12, 26 -- X-YMail-OSG: ETKpnmsVM1l7e3kaiji5LPs16AnfoB9Zql9hjT3KX680ZwmIJYW8Ey7rGbPcPlQaqEp5pKnNyI3EjjYeB2dq.wWt1Yqjm8vIGoPyF1ksBDuIPbNf7h3.GNNht398tZ2w63H52s_zaWx54N.hxq69UsJbsoZj4yjXU05RLgXh48iKoNCDQ882WSyv_Z6lYb3OjdXlt2PepB6.BE5Hj99dLxbuwspWKt3zQpBegGSaoSGjciAs4m4E0xNs.ul2Wp5uQiFSmQnnpzp24LERelg-
12, 26 -- Received: from [132.239.85.200] by web46103.mail.sp1.yahoo.com via HTTP; Thu, 30 Apr 2009 15:22:08 PDT
12, 26 -- X-Mailer: YahooMailClassic/5.2.20 YahooMailWebService/0.7.289.1
12, 26 -- Date: Thu, 30 Apr 2009 15:22:08 -0700 (PDT)
12, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 26 -- Subject: Processing Fat for Paraffin
12, 26 -- To: HistoNet {histonet-at-lists.utsouthwestern.edu} ,
12, 26 -- MSA BB {Microscopy-at-microscopy.com}
12, 26 -- MIME-Version: 1.0
12, 26 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 30 Apr 2009 18:52:33 -0500
Subject: [Microscopy] Hitachi S-4700 experiences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all:

For users of the Hitachi S-4700 SEM, I would
appreciate feedback about what you think about
this system.

} } Does the S-4700 come with an operator's manual, schematics
} } and maintenance manual? Does Hitachi or you change
} } the tip? What is the cost of the tip and/or the
} } maintenance contract? How often do you have to
} } flash the tip? Do you have the bakeout materials? Have you used this?
} }
} } What are the dry pumps for this system if you have them? What
} } OS and GUI version and generation are in the
} } system? What is the max digital pixel capture values?
} }
} } Is there a separate user and service login? If so, do
} } you have the service login password? What is the maintenance
} } history of your tool? I.e., down a lot or up a lot?

Do you have EDS and how satisfied are you with the
SEM's ability to do EDS? What is the analytical
WD for EDS?

Does the specimen interchange load lock work well? Does it
support FEI/LEO/Amray/Zeiss 3.1mm pin stubs without a lot
of hassle?

What does a basic maintenance contract cost and what does
it cover? If you don't have a contract, how responsive is
Hitachi to your specific problems that you cannot fix?
What about GUI upgrades? Can you get these without a
contract?

Finally, what do you think about the cold FE versus the
Schottky FE? Have you had problems with stability for
lengthy EDS mapping?

All input appreciated--off-line is probably preferred.

gary g.



==============================Original Headers==============================
11, 17 -- From gary-at-gaugler.com Thu Apr 30 18:52:33 2009
11, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3UNqU3k003280
11, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 18:52:32 -0500
11, 17 -- Message-Id: {200904302352.n3UNqU3k003280-at-ns.microscopy.com}
11, 17 -- Received: (qmail 3591 invoked from network); 30 Apr 2009 17:04:40 -0700
11, 17 -- Received: by simscan 1.1.0 ppid: 3587, pid: 3588, t: 0.0802s
11, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 17 -- by smtp1 with SMTP; 30 Apr 2009 17:04:40 -0700
11, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 17 -- Date: Thu, 30 Apr 2009 16:52:28 -0700
11, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
11, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 17 -- Subject: Hitachi S-4700 experiences
11, 17 -- Mime-Version: 1.0
11, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: HStahlberg-at-ucdavis.edu
Date: Fri, 1 May 2009 08:08:37 -0500
Subject: [Microscopy] Postdoc and PhD position in cryo-electron tomography in Basel, Switzerland.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A postdoctoral position and a graduate student position are available
in the group of Henning Stahlberg in the laboratory for Cellular
Imaging and Nano Analytics at the University Basel in Switzerland.
The project involves the 3D structural analysis of several samples
from the areas of neurology and bacterial infection biology, using
cryo-electron tomography and computer image processing as major methods.
The positions are available immediately, long-term funding is secured.
Equipment includes a FEI Titan Krios with autoloader, 4K CCD and GIF,
as well as several other instruments. Basel is a culturally rich and
beautiful city at the border between Switzerland, France and Germany.
For further information, please contact Henning Stahlberg (Henning.Stahlberg-at-unibas.ch
, +41-61-387 32 62).

___________________________________________________

Henning Stahlberg
Center for Cellular Imaging and Nanoanalytics (C-CINA)
Structural Biology and Biophysics, Biozentrum,
WRO-1058, Mattenstrasse 26
University Basel, CH-4058 Basel, Switzerland
Tel: +41 - 61 - 387 32 62 (office)
Tel: +41 - 61 - 387 32 27 (administrative assistant)
mailto:Henning.Stahlberg-at-unibas.ch
http://stahlberglab.org
http://2dx.org
___________________________________________________


==============================Original Headers==============================
6, 21 -- From HStahlberg-at-ucdavis.edu Fri May 1 08:08:37 2009
6, 21 -- Received: from mx3.ucdavis.edu (mx3.ucdavis.edu [128.120.32.33])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n41D8ZDw024769
6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 May 2009 08:08:36 -0500
6, 21 -- Received: from bs-cina-bookable-01.ethz.ch (bs-cina-bookable-01.ethz.ch [129.132.128.90])
6, 21 -- (authenticated bits=0)
6, 21 -- by mx3.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n41D8LgE000551
6, 21 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 May 2009 06:08:33 -0700 (PDT)
6, 21 -- Message-Id: {638846CB-F2B1-454C-932B-763753DC87A9-at-ucdavis.edu}
6, 21 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu}
6, 21 -- To: Microscopy-at-microscopy.com
6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- Mime-Version: 1.0 (Apple Message framework v930.4)
6, 21 -- Subject: Postdoc and PhD position in cryo-electron tomography in Basel, Switzerland.
6, 21 -- Date: Fri, 1 May 2009 15:08:20 +0200
6, 21 -- X-Mailer: Apple Mail (2.930.4)
6, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av8
6, 21 -- X-Virus-Status: Clean
6, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.33
==============================End of - Headers==============================




From: Bob.Price-at-uscmed.sc.edu
Date: Fri, 1 May 2009 15:20:10 -0500
Subject: [Microscopy] viaWWW: Basic Confocal Workshop @ University of South Carolina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-----------------------------------------------------------

Email: Bob.Price-at-uscmed.sc.edu
Name: Bob Price

Organization: USC School of Medicine

Title-Subject: [Filtered] Basic Confocal Workshop -at- University of
South Carolina

Question: There are still some slots available in this year's Basic
Confocal Workshop hosted by the University of South Carolina. This
year's workshop will be from June 15-19, 2009 and will include a
series of lectures on the theory and applications of confocal
microscopy, specimen preparation, processing confocal images in
Photoshop, and 3D reconstructions using AMIRA. Students will be able
to process triple labeled samples (cell cultures and sections) on
site or bring their own samples to the workshop.

Faculty will include Drs. Jay Jerome (Vanderbilt), Ralph Albrecht
(Univ Wisconsin-Madison), John Mackenzie (North Carolina State Univ),
Tom TrusK (Medical Univ South Carolina) and myself.

Instruments and applications experts from Leica, Nikon, Olympus,
Perkin Elmer, Photometrics, and Zeiss will be available for hands on
training and imaging of samples.

For those contemplating instrumentation proposals as part of the
stimulus or other funding opportunities this is an excellent
opportunity to see several systems side by side and to collect
preliminary data on their instrument of choice.

For further information and registration go to:
http://dba.med.sc.edu/irf/price/irf/irf.htm or contact Anna McNeal
(Anna.McNeal-at-uscmed.sc.edu {mailto:Anna.McNeal-at-uscmed.sc.edu} )

Bob

Bob Price
Research Professor
Dept Cell Biol and Anat
USC School of Medicine
6439 Garner's Ferry Road
Columbia, SC 29208
Tel: 803-733-3392
Admin Tel: 803-253-5822
Fax: 803-733-3212

Login Host: 130.202.238.72
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Fri May 1 15:20:10 2009
12, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n41KK9Iv029680
12, 11 -- for {microscopy-at-microscopy.com} ; Fri, 1 May 2009 15:20:09 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240802c62109d9bf03-at-[206.69.208.22]}
12, 11 -- Date: Fri, 1 May 2009 15:20:08 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: Bob.Price-at-uscmed.sc.edu (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Basic Confocal Workshop -at- University of South Carolina
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kleopullin-at-pacbell.net
Date: Sun, 3 May 2009 19:44:59 -0500
Subject: [Microscopy] Planar structures on IC - RAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mcintyre-at-optics.rochester.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mcintyre-at-optics.rochester.edu
Name: Brian McIntyre

Organization: Univ of Rochester

Title-Subject: [Filtered] Student Web Page Projects

Question: Hi all-

As usual our Electron Microscopy class projects have been posted on
our webserver. If you'd like to take a look go here:

http://www.optics.rochester.edu/workgroups/cml/opt307/spr09/

Brian

Login Host: 66.66.201.62
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Sat May 2 08:33:07 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n42DX6YA016246
9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 2 May 2009 08:33:06 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c621fbda7864-at-[206.69.208.22]}
9, 11 -- Date: Sat, 2 May 2009 08:33:05 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: mcintyre-at-optics.rochester.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Student Web Page Projects
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From li_song33-at-excite.com Sun May 3 04:16:24 2009
Return-Path: {li_song33-at-excite.com}
Received: from google.com (fla2404-015.wind.ne.jp [218.223.57.15])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n439GMuv013732
for {microscopylistserverarchive-at-microscopy.com} ; Sun, 3 May 2009 04:16:23 -0500
Received: from [125.92.74.69] (HELO google.com)
by hollow-wisehead.us; Sun, 3 May 2009 18:16:21 +0900


I'm shooting electron micrographs of mostly planar structures on an IC which I assume is some kind of RAM. Initially I started looking at microprocessors, and, on-line I had found a number of diagrams that labeled areas of the microprocessor as SRAM, DRAM, ALU, etc. I switched to RAM assuming the same information would be readily available for RAM, i.e. some type of equivalent lay-out of various RAM chips at different magnifications but cannot find anything like the microprocessor diagrams:

http://www.chip-architect.com/news/2003_09_21_Detailed_Architecture_of_AMDs_64bit_Core.html

Ditto light microscopy along the lines of fly logic, a reverse engineering firm that etches and uncovers, then images the various layers of chips:

http://www.flylogic.net/blog/?p=32

I figured all I have to know is where's the input area/encoding/memory/decoding/output, but I didn't translate this into identifying these areas from circuitry.

The chip has the number 7025 on it, and its metal layers number is 105, and it was probably made in 1994 or 1995. In the SE image number 0002 the upper left, where the pads are, is the middle of the chip, and, the large main memory area of the chip is the dark area on the bottom central third of the micrograph.

Can anyone tell me any information about these micrographs? Or where I can find information about planar RAM circuitry at various magnifications? Or if I'm even asking the right questions? I put some micrographs on the web at:

http://www.djwatt.com/

I did not first adjust to web resolution, so, to look at them as micrographs, it might be better to just copy and paste in a word document as they appear awful large on the screen. They should paste as nice 4" high micrographs into some type of document.

Probably off-line responses are better.


Thanks, Kleo

KLeoPullin-at-pacbell.net

==============================Original Headers==============================
14, 21 -- From kleopullin-at-pacbell.net Sun May 3 19:44:59 2009
14, 21 -- Received: from web83408.mail.sp1.yahoo.com (web83408.mail.sp1.yahoo.com [69.147.64.56])
14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n440iweU009479
14, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 3 May 2009 19:44:59 -0500
14, 21 -- Received: (qmail 24041 invoked by uid 60001); 4 May 2009 00:44:58 -0000
14, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1241397898; bh=VK0hFu/6ztRFxM+U/877rwx/viqiImV435xv9gHsQx4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=VnOz6r0lGsz3UMupMhceznL+Y0XtzdP3LNFWdXVnLWt5jCYpjS8zIy/AyN7BaQg+KXOFvtT2q0hXe8neOSCm45wgRwQynvFxKmsL9BIOrxa6O2zM5FKCELLLzqcS21E+53+2pA0TILkWDDidW3TIXbBIEObPq48Tgr+VrCkx5RM=
14, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 21 -- s=s1024; d=pacbell.net;
14, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type;
14, 21 -- b=xmV3ZapcGz43FoAJNS2lg/yGfEUbp1QmnBuz8qJpEWVdNrzgU0f+5KknWhTI0oHSy90zGoLfE6x804Blk93F8070gdPI+jh4pTGiAsk93Mv8GmLiYBYEm5YMzalZJo61U8k5S5JdfIyM/nXZ8klmCLxYgL/dM72a1atKfvQHtzw=;
14, 21 -- Message-ID: {492913.22296.qm-at-web83408.mail.sp1.yahoo.com}
14, 21 -- X-YMail-OSG: YBAwSoYVM1mOO_TCUkhz_xu83MqTadf0Rno0.DjkhVbE3zcTR98XfvHMj0N_grLTTuDSg5653ELpXAego5g1m3YzUsA2WQ_.6.9M6rpBxtqULeFwAf965MoWAbgJ5IUcmSn6czfzVm7Vul3GtCMw_f9u10w1RHOe2Wpk21VdM_7yKvz.N0Lof.yGunGac8_te9ddjIBnNBZqMyTr2GQcz7CYL4YEEjcrvbi7dLi9y9Bzwbzwbt0lhon_ckX9s1jRG._suegreka48BUxn5ynrYI8I0xthQA24avylLl7K7wu2NEAlbuidnhMJS0H.Eugbe.G.eVtnQrnmwK5SrXZRfU-
14, 21 -- Received: from [69.226.103.38] by web83408.mail.sp1.yahoo.com via HTTP; Sun, 03 May 2009 17:44:58 PDT
14, 21 -- X-Mailer: YahooMailWebService/0.7.289.1
14, 21 -- Date: Sun, 3 May 2009 17:44:58 -0700 (PDT)
14, 21 -- From: Kleo Pullin {kleopullin-at-pacbell.net}
14, 21 -- Reply-To: kleopullin-at-pacbell.net
14, 21 -- Subject: Planar structures on IC - RAM
14, 21 -- To: Microscopy-at-microscopy.com
14, 21 -- MIME-Version: 1.0
14, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: weis183-at-yahoo.fr
Date: Mon, 4 May 2009 04:17:13 -0500
Subject: [Microscopy] Re : Re: Basic Materials TEM/SEM/ LM book ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Not a book (the book is in french to my knowledge but the english version is expected in august 2009) but a good web site for the preparation of TEM samples:
http://temsamprep.in2p3.fr/techniques.php?lang=eng

Patrick

} What I have a hard time finding is a reference on specimen
} preparation for materials science. Be interesting to see if anyone
} posts such a book.

Phil

} Hello.  We have traditionally been a Life Sciences Imaging core facility on
} campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding
} ourselves leaning more towards the Material Sciences.  As such, we would
} like to augment our Center's reference library with books relating to the
} imaging and analytical side of microscopy
}
} I was wondering if I could get ideas on basic book requirements that
} Material scientists would look at as basic references and might be
} considered indispensable in a Materials oriented research center. Thanks for
} any help! Mark
}
}
}
} Mark Grimson, PhD
} Manager, The Imaging Center
} c/o The Department of Biological Sciences
} Flint and Main
} Texas Tech University
} Lubbock, TX    79409-3131
}
} mark.grimson-at-ttu.edu
} 806-742-3722  x235 (Office)
} 806-252-3879 (Cell)
} 806-742-2963 (FAX)
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 23 -- From oshel1pe-at-cmich.edu Fri Apr 24 14:07:18 2009
4, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7IKc022534
4, 23 --     for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:18 -0500
4, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 23 --     by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3OJ7Ivo011624
4, 23 --     for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:07:18 -0400
4, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 23 --     Fri, 24 Apr 2009 15:07:17 -0400
4, 23 -- Mime-Version: 1.0
4, 23 -- Message-Id: {f06240816c617be48e46a-at-[141.209.160.249]}
4, 23 -- Date: Fri, 24 Apr 2009 15:07:11 -0400
4, 23 -- To: Microscopy-at-microscopy.com
4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 23 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas
4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 23 -- X-OriginalArrivalTime: 24 Apr 2009 19:07:18.0060 (UTC) FILETIME=[E3035EC0:01C9C50F]
4, 23 -- X-Canit-CHI2: 0.00
4, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 23 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 23 -- X-CanItPRO-Stream: default
4, 23 -- X-Canit-Stats-ID: 12386593 - 56aaaf500e5c
4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================






==============================Original Headers==============================
11, 24 -- From weis183-at-yahoo.fr Mon May 4 04:17:13 2009
11, 24 -- Received: from web24615.mail.ird.yahoo.com (web24615.mail.ird.yahoo.com [212.82.104.172])
11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n449HCDW010816
11, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 4 May 2009 04:17:13 -0500
11, 24 -- Received: (qmail 81041 invoked by uid 60001); 4 May 2009 09:17:12 -0000
11, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1241428632; bh=a14g+EjRjLXZkVC7S23toqjM/0hZXPyS6Zq25ONqE0E=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=DKUrT1XcbQ10+OqUZRjFi3h88Sc1M4W+bjCsHzyuwK9+gtMPvcI2KqwIBhdy8QCw2r3yIev/DWbDhSmtWS7DNafsQW7B04VU0Q186V+d/Od9XP/3H+PI/s5LwVITCBdwYvdzWdUArSnGGU6CvuadczJqNPpiLDGns3hPC/e/GIY=
11, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
11, 24 -- s=s1024; d=yahoo.fr;
11, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
11, 24 -- b=F+o7WPmcQ5PbsU0Y77kUEGhFn0+0kQAvdVgEmGmasqG++P8IzGn2FRdAC85VzpwxH1DvQP4dWDxswX9AxDDgN5UOb/B8ABg7sIilGjjZ+M+Fzvs8v1W64G0PjbOWnbKEiQ8wZjw2cxuHIz64J0WCZEBMXIodLOKFciD1O0pQvYg=;
11, 24 -- Message-ID: {252345.80778.qm-at-web24615.mail.ird.yahoo.com}
11, 24 -- X-YMail-OSG: YWISGaUVM1nIJJF5mrbjJqIK96AV5rqxv_D0coIy89uJjmhcbagHE0Cs
11, 24 -- Received: from [147.210.80.23] by web24615.mail.ird.yahoo.com via HTTP; Mon, 04 May 2009 02:17:11 PDT
11, 24 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
11, 24 -- References: {200904241910.n3OJAOB4028375-at-ns.microscopy.com}
11, 24 -- Date: Mon, 4 May 2009 02:17:11 -0700 (PDT)
11, 24 -- From: Patrick Weisbecker {weis183-at-yahoo.fr}
11, 24 -- Subject: Re : [Microscopy] Re: Basic Materials TEM/SEM/ LM book ideas
11, 24 -- To: Microscopy-at-microscopy.com
11, 24 -- In-Reply-To: {200904241910.n3OJAOB4028375-at-ns.microscopy.com}
11, 24 -- MIME-Version: 1.0
11, 24 -- Content-Type: text/plain; charset=iso-8859-1
11, 24 -- Content-Transfer-Encoding: 8bit
11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n449HCDW010816
==============================End of - Headers==============================




From: jerzy.gazda-at-ceriumlabs.com
Date: Mon, 4 May 2009 13:17:02 -0500
Subject: [Microscopy] RE: Planar structures on IC - RAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kleo,
The description you provided for main portions of the circuit are
correct. However, you are looking at the tom most metal layer (with
external pad connections. The actual circuitry corresponding to the RAM
cell are at or just above Si substrate (Poly Si gates, and W-contacts to
Metal-1 (W or Al). If this is 1995 vintage RAM, there will be about 6
transistors (0.35-0.5um gate width) making the circuit for the basic bit
of storage (inverter circuit). To uncover that layout you will need to
de-process the device (remove the top 3-5 metal layers), then image
Metal-1, contact, and poly layers. Then draw the diagrams that are
described by Fly-logic people.

The Anthlon64 information, is complete on the design part of the device,
however they do not describe the basic RAM cell. The device has actually
3 areas of SRAM caches (L0, L1, and L2-largest array at top of the shown
die) associated with the core. Also the Athlon64 is made on SOI, with
poly gates at 0.09um or smaller and with Cu dual-damasceene
metallization layers (9 of them). Remarkably enough the basic RAM cell
layout is probably the same ... I used to help make those little things
at AMD.

Have Fun, this is not a complicated work, but requires a lot of patience
at the polishing wheel, chemical etching or RIE, and low KV FEG-SEM
imaging.


Jerzy
***************************************************
Jerzy Gazda Ph.D.
Section Manager - TEM, FIB, SEM
Cerium Laboratories LLC
5204 E. Ben White Blvd. MS-512
Austin, TX 78741

(512) 934-5185 vm
(512) 622-6600 pgr

www.ceriumlabs.com


------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


I'm shooting electron micrographs of mostly planar structures on an IC
which I assume is some kind of RAM. Initially I started looking at
microprocessors, and, on-line I had found a number of diagrams that
labeled areas of the microprocessor as SRAM, DRAM, ALU, etc. I switched
to RAM assuming the same information would be readily available for RAM,
i.e. some type of equivalent lay-out of various RAM chips at different
magnifications but cannot find anything like the microprocessor
diagrams:

http://www.chip-architect.com/news/2003_09_21_Detailed_Architecture_of_A
MDs_64bit_Core.html

Ditto light microscopy along the lines of fly logic, a reverse
engineering firm that etches and uncovers, then images the various
layers of chips:

http://www.flylogic.net/blog/?p=32

I figured all I have to know is where's the input
area/encoding/memory/decoding/output, but I didn't translate this into
identifying these areas from circuitry.

The chip has the number 7025 on it, and its metal layers number is 105,
and it was probably made in 1994 or 1995. In the SE image number 0002
the upper left, where the pads are, is the middle of the chip, and, the
large main memory area of the chip is the dark area on the bottom
central third of the micrograph.

Can anyone tell me any information about these micrographs? Or where I
can find information about planar RAM circuitry at various
magnifications? Or if I'm even asking the right questions? I put some
micrographs on the web at:

http://www.djwatt.com/

I did not first adjust to web resolution, so, to look at them as
micrographs, it might be better to just copy and paste in a word
document as they appear awful large on the screen. They should paste as
nice 4" high micrographs into some type of document.


Probably off-line responses are better.


Thanks, Kleo

KLeoPullin-at-pacbell.net

==============================Original
Headers==============================
14, 21 -- From kleopullin-at-pacbell.net Sun May 3 19:44:59 2009
14, 21 -- Received: from web83408.mail.sp1.yahoo.com
(web83408.mail.sp1.yahoo.com [69.147.64.56])
14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n440iweU009479
14, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 3 May 2009
19:44:59 -0500
14, 21 -- Received: (qmail 24041 invoked by uid 60001); 4 May 2009
00:44:58 -0000
14, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=pacbell.net; s=s1024; t=1241397898;
bh=VK0hFu/6ztRFxM+U/877rwx/viqiImV435xv9gHsQx4=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To
:MIME-Version:Content-Type;
b=VnOz6r0lGsz3UMupMhceznL+Y0XtzdP3LNFWdXVnLWt5jCYpjS8zIy/AyN7BaQg+KXOFvt
T2q0hXe8neOSCm45wgRwQynvFxKmsL9BIOrxa6O2zM5FKCELLLzqcS21E+53+2pA0TILkWDD
idW3TIXbBIEObPq48Tgr+VrCkx5RM=
14, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 21 -- s=s1024; d=pacbell.net;
14, 21 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To
:MIME-Version:Content-Type;
14, 21 --
b=xmV3ZapcGz43FoAJNS2lg/yGfEUbp1QmnBuz8qJpEWVdNrzgU0f+5KknWhTI0oHSy90zGo
LfE6x804Blk93F8070gdPI+jh4pTGiAsk93Mv8GmLiYBYEm5YMzalZJo61U8k5S5JdfIyM/n
XZ8klmCLxYgL/dM72a1atKfvQHtzw=;
14, 21 -- Message-ID: {492913.22296.qm-at-web83408.mail.sp1.yahoo.com}
14, 21 -- X-YMail-OSG:
YBAwSoYVM1mOO_TCUkhz_xu83MqTadf0Rno0.DjkhVbE3zcTR98XfvHMj0N_grLTTuDSg565
3ELpXAego5g1m3YzUsA2WQ_.6.9M6rpBxtqULeFwAf965MoWAbgJ5IUcmSn6czfzVm7Vul3G
tCMw_f9u10w1RHOe2Wpk21VdM_7yKvz.N0Lof.yGunGac8_te9ddjIBnNBZqMyTr2GQcz7CY
L4YEEjcrvbi7dLi9y9Bzwbzwbt0lhon_ckX9s1jRG._suegreka48BUxn5ynrYI8I0xthQA2
4avylLl7K7wu2NEAlbuidnhMJS0H.Eugbe.G.eVtnQrnmwK5SrXZRfU-
14, 21 -- Received: from [69.226.103.38] by web83408.mail.sp1.yahoo.com
via HTTP; Sun, 03 May 2009 17:44:58 PDT
14, 21 -- X-Mailer: YahooMailWebService/0.7.289.1
14, 21 -- Date: Sun, 3 May 2009 17:44:58 -0700 (PDT)
14, 21 -- From: Kleo Pullin {kleopullin-at-pacbell.net}
14, 21 -- Reply-To: kleopullin-at-pacbell.net
14, 21 -- Subject: Planar structures on IC - RAM
14, 21 -- To: Microscopy-at-microscopy.com
14, 21 -- MIME-Version: 1.0
14, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of -
Headers==============================


==============================Original Headers==============================
23, 28 -- From Jerzy.Gazda-at-spansion.com Mon May 4 13:17:02 2009
23, 28 -- Received: from usausmgw02.spansion.com (usausmgw02.spansion.com [12.110.209.162])
23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44IH1D1020347
23, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 4 May 2009 13:17:01 -0500
23, 28 -- X-IronPort-AV: E=McAfee;i="5300,2777,5605"; a="3300501"
23, 28 -- Received: from usausexbh1.spansion.com ([10.248.26.58])
23, 28 -- by usausmgw02.spansion.com with ESMTP; 04 May 2009 11:17:01 -0700
23, 28 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959);
23, 28 -- Mon, 4 May 2009 13:17:01 -0500
23, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
23, 28 -- Content-class: urn:content-classes:message
23, 28 -- MIME-Version: 1.0
23, 28 -- Content-Type: text/plain;
23, 28 -- charset="us-ascii"
23, 28 -- Subject: RE: [Microscopy] Planar structures on IC - RAM
23, 28 -- Date: Mon, 4 May 2009 13:17:00 -0500
23, 28 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158738730-at-USAUSEXMBPF1.spansion.com}
23, 28 -- In-Reply-To: {200905040054.n440sL8n021003-at-ns.microscopy.com}
23, 28 -- X-MS-Has-Attach:
23, 28 -- X-MS-TNEF-Correlator:
23, 28 -- Thread-Topic: [Microscopy] Planar structures on IC - RAM
23, 28 -- Thread-Index: AcnMUt1ilwotOqR2QgyQAEMKK+J6SgAj7k6g
23, 28 -- References: {200905040054.n440sL8n021003-at-ns.microscopy.com}
23, 28 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com}
23, 28 -- To: {kleopullin-at-pacbell.net} , {Microscopy-at-microscopy.com}
23, 28 -- X-OriginalArrivalTime: 04 May 2009 18:17:01.0610 (UTC) FILETIME=[853320A0:01C9CCE4]
23, 28 -- Content-Transfer-Encoding: 8bit
23, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44IH1D1020347
==============================End of - Headers==============================




From: kintzjd-at-rocketmail.com
Date: Mon, 4 May 2009 14:31:57 -0500
Subject: [Microscopy] Need 40 mm diameter metallurgical mount holder for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a source for a metallurgical mount holder that will accommodate 40 mm diameter metallurgical mounts? Pella has them for 25, 32, 38, and 51 mm diameter metallurgical mounts, but not 40. It's for an SEM, and I need a 3.2 mm pin.
Thanks,
 
Joe





==============================Original Headers==============================
5, 22 -- From kintzjd-at-rocketmail.com Mon May 4 14:31:57 2009
5, 22 -- Received: from web53311.mail.re2.yahoo.com (web53311.mail.re2.yahoo.com [206.190.49.101])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n44JVtM5006627
5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:31:56 -0500
5, 22 -- Received: (qmail 23425 invoked by uid 60001); 4 May 2009 19:31:54 -0000
5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=rocketmail.com; s=s1024; t=1241465514; bh=02fxjLnazLVe+i25jlRdc++FVAIucAEJThCeDOP8qkU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=k6WKvD3bmii7vRDJgCABHxiovAQ9+d+yxsPVHEBP5keLeY5ewksAKX1mzAE6DHF0BtU+rWSKiEIu+F1Zd22oU0002fETP6Z0O0yuT+AQSUsvqBt1H0RFfzO30DNB+06r0UPQzZKrAKbzxjylTYx+iaO8+c7Jqjojzwae9Uobhn4=
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=rocketmail.com;
5, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
5, 22 -- b=YTaRUfTc2jhQPiXGROeYlWIhFDlOg2W7ZoUl1un5T8WBsH4DOOLxfJVwQ+zY/4xBgdXnkq2a1ZaXANWoFGdq71aasXbYLuB0idJl0JwnFVvdzv77XSqfybMY3liCaAMyWIcKvnWnLs7SKVpB3/oEFGaaGrRXyeQj8KzOUyemU6Q=;
5, 22 -- Message-ID: {379998.19725.qm-at-web53311.mail.re2.yahoo.com}
5, 22 -- X-YMail-OSG: rsXG50EVM1msF6nGZsaCN9iklXG.xcG89FZ.Sxux5PnOwaF4np1Gcf0QTVeHrxDmVLR_xIZ9F82dC47pWilYSlsWolz.J3.QGEjnz1tdUxC7RAzgYDHDmpAMaFMvhBb6Mw2NN4RlEuxD.1HVsv23TYsvmBJSro6MSeG2y36Qiij6ffn03CWau.nmhvD8tdTwMnTQitJfkmeLwG0qkC3T7HerqJWkDnDiGBK4OPd.ztqstRLxPEV7
5, 22 -- Received: from [24.149.204.114] by web53311.mail.re2.yahoo.com via HTTP; Mon, 04 May 2009 12:31:54 PDT
5, 22 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
5, 22 -- Date: Mon, 4 May 2009 12:31:54 -0700 (PDT)
5, 22 -- From: Joseph Kintz {kintzjd-at-rocketmail.com}
5, 22 -- Subject: Need 40 mm diameter metallurgical mount holder for SEM
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=iso-8859-1
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44JVtM5006627
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Mon, 4 May 2009 14:48:14 -0500
Subject: [Microscopy] Need 40 mm diameter metallurgical mount holder for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe,
If all else fails, use double sided tape on a 1" stub. Unless you're
planning a lot of work near 90 deg tilt, it should work.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: kintzjd-at-rocketmail.com [mailto:kintzjd-at-rocketmail.com]
Sent: Monday, May 04, 2009 3:35 PM
To: kenconverse-at-qualityimages.biz


Does anyone know of a source for a metallurgical mount holder that will
accommodate 40 mm diameter metallurgical mounts? Pella has them for 25, 32,
38, and 51 mm diameter metallurgical mounts, but not 40. It's for an SEM,
and I need a 3.2 mm pin.
Thanks,
 
Joe





==============================Original Headers==============================
5, 22 -- From kintzjd-at-rocketmail.com Mon May 4 14:31:57 2009
5, 22 -- Received: from web53311.mail.re2.yahoo.com
(web53311.mail.re2.yahoo.com [206.190.49.101])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n44JVtM5006627
5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:31:56
-0500
5, 22 -- Received: (qmail 23425 invoked by uid 60001); 4 May 2009 19:31:54
-0000
5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=rocketmail.com; s=s1024; t=1241465514;
bh=02fxjLnazLVe+i25jlRdc++FVAIucAEJThCeDOP8qkU=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
b=k6WKvD3bmii7vRDJgCABHxiovAQ9+d+yxsPVHEBP5keLeY5ewksAKX1mzAE6DHF0BtU+rWSKiE
Iu+F1Zd22oU0002fETP6Z0O0yuT+AQSUsvqBt1H0RFfzO30DNB+06r0UPQzZKrAKbzxjylTYx+ia
O8+c7Jqjojzwae9Uobhn4=
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=rocketmail.com;
5, 22 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
5, 22 --
b=YTaRUfTc2jhQPiXGROeYlWIhFDlOg2W7ZoUl1un5T8WBsH4DOOLxfJVwQ+zY/4xBgdXnkq2a1Z
aXANWoFGdq71aasXbYLuB0idJl0JwnFVvdzv77XSqfybMY3liCaAMyWIcKvnWnLs7SKVpB3/oEFG
aaGrRXyeQj8KzOUyemU6Q=;
5, 22 -- Message-ID: {379998.19725.qm-at-web53311.mail.re2.yahoo.com}
5, 22 -- X-YMail-OSG:
rsXG50EVM1msF6nGZsaCN9iklXG.xcG89FZ.Sxux5PnOwaF4np1Gcf0QTVeHrxDmVLR_xIZ9F82d
C47pWilYSlsWolz.J3.QGEjnz1tdUxC7RAzgYDHDmpAMaFMvhBb6Mw2NN4RlEuxD.1HVsv23TYsv
mBJSro6MSeG2y36Qiij6ffn03CWau.nmhvD8tdTwMnTQitJfkmeLwG0qkC3T7HerqJWkDnDiGBK4
OPd.ztqstRLxPEV7
5, 22 -- Received: from [24.149.204.114] by web53311.mail.re2.yahoo.com via
HTTP; Mon, 04 May 2009 12:31:54 PDT
5, 22 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1
5, 22 -- Date: Mon, 4 May 2009 12:31:54 -0700 (PDT)
5, 22 -- From: Joseph Kintz {kintzjd-at-rocketmail.com}
5, 22 -- Subject: Need 40 mm diameter metallurgical mount holder for SEM
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=iso-8859-1
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n44JVtM5006627
==============================End of - Headers==============================




==============================Original Headers==============================
17, 25 -- From kenconverse-at-qualityimages.biz Mon May 4 14:48:13 2009
17, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44JmCnJ021183
17, 25 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:48:13 -0500
17, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
17, 25 -- with ESMTP
17, 25 -- id {20090504194809481.RLKH19475-at-cdptpa-omta01.mail.rr.com} ;
17, 25 -- Mon, 4 May 2009 19:48:09 +0000
17, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
17, 25 -- To: {kintzjd-at-rocketmail.com} , "MSA Listserver" {microscopy-at-microscopy.com}
17, 25 -- Subject: RE: [Microscopy] Need 40 mm diameter metallurgical mount holder for SEM
17, 25 -- Date: Mon, 4 May 2009 15:48:03 -0400
17, 25 -- Message-ID: {A1A83CC8020544D5AD51097E292FCF7F-at-Ken}
17, 25 -- MIME-Version: 1.0
17, 25 -- Content-Type: text/plain;
17, 25 -- charset="iso-8859-1"
17, 25 -- X-Priority: 3 (Normal)
17, 25 -- X-MSMail-Priority: Normal
17, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
17, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
17, 25 -- In-Reply-To: {200905041935.n44JZPlS010643-at-ns.microscopy.com}
17, 25 -- Thread-Index: AcnM73ij3ViyKmg3Rp6+9DX/ZngioAAAVMRQ
17, 25 -- Importance: Normal
17, 25 -- Content-Transfer-Encoding: 8bit
17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44JmCnJ021183
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Mon, 4 May 2009 18:40:51 -0500
Subject: [Microscopy] Re: viaWWW: TEM: Magnetic Field Cancellation Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roseann

Thanks for your interest. Based on the fairly limited information I have,
the machine is performing as per its factory testing and specifications. So
any wobble I am seeing in the beam, is most likely down to external fields.

Regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au



On 1/5/09 12:16 AM, "Roseann Csencsits" {RCsencsits-at-lbl.gov} wrote:

} Hi David,
} Has the manufacturer verified that all of their power supplies are
} within spec?
} Did performance prove better in the factory?
}
} Roseann Csencsits, PhD
} Scientist in Charge - Donner TEM Facility
} Lawrence Berkeley Lab 01-365
} 1 Cyclotron Road
} Berkeley, CA 94720
} 510-486-4548
}
}
}
}
}
}
}
} On Apr 30, 2009, at 6:41 AM, david.mitchell-at-emu.usyd.edu.au wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} } ---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when
} } replying
} } please copy both david.mitchell-at-emu.usyd.edu.au as well as the
} } MIcroscopy Listserver
} } ---------------------------------------------------------------------------
} }
} } Email: david.mitchell-at-emu.usyd.edu.au
} } Name: David Mitchell
} }
} } Organization: EM Unit, University of Sydney
} }
} } Title-Subject: [Filtered] TEM: Magnetic Field Cancellation Systems
} }
} } Question: Dear All
} }
} } We are currently commissioning a new 200kV TEM/STEM with an in-column
} } filter. The room only just met environmental specs with respect to
} } magnetic field levels, and the new instrument does meets performance
} } specs in terms of STEM resolution. However, I think it could be
} } better. A fixed probe at 1 million times does show evidence of
} } external magnetic field interference. These is some jitter in the
} } 1-10Hz range of about 1nm amplitude, and there is much lower
} } frequency drift over longer time frames of } 1 minute. We have
} } measured AC fields in various planes, at typically 1mG or less, but
} } up to 2.2mG in some instances. I have used a Lundgren field
} } compensation system in a lab which was way out of spec (15mG), and so
} } realise that they can make a huge difference in those situations.
} } However, does anyone have experience with such systems in relatively
} } low field environments such as I have? Is any potential improvement
} } in STEM performance going to be worth the ca $50k investment?
} }
} } Thanks in advance,
} }
} } Dave Mitchell
} }
} } Login Host: 129.78.64.103
} } ---------------------------------------------------------------------------
} }
} } ==============================Original
} } Headers==============================
} } 9, 11 -- From zaluzec-at-microscopy.com Thu Apr 30 08:33:32 2009
} } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n3UDXV58022708
} } 9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 08:33:32
} } -0500
} } 9, 11 -- Mime-Version: 1.0
} } 9, 11 -- Message-Id: {p06240800c61f58f9a856-at-[206.69.208.22]}
} } 9, 11 -- Date: Thu, 30 Apr 2009 08:33:30 -0500
} } 9, 11 -- To: microscopy-at-microscopy.com
} } 9, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of
} } MicroscopyListserver)
} } 9, 11 -- Subject: viaWWW: TEM: Magnetic Field Cancellation Systems
} } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} } format="flowed"
} } ==============================End of -
} } Headers==============================
}


==============================Original Headers==============================
13, 26 -- From david.mitchell-at-emu.usyd.edu.au Mon May 4 18:40:51 2009
13, 26 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44NeoRv016508
13, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 May 2009 18:40:51 -0500
13, 26 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1])
13, 26 -- by localhost (Postfix) with SMTP id 70540103970;
13, 26 -- Tue, 5 May 2009 09:40:47 +1000 (EST)
13, 26 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19])
13, 26 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id 0F35D103926;
13, 26 -- Tue, 5 May 2009 09:40:46 +1000 (EST)
13, 26 -- Received: from 172.17.185.136 ([172.17.185.136]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ;
13, 26 -- Mon, 4 May 2009 23:40:45 +0000
13, 26 -- User-Agent: Microsoft-Entourage/11.3.6.070618
13, 26 -- Date: Tue, 05 May 2009 09:40:45 +1000
13, 26 -- Subject: Re: [Microscopy] viaWWW: TEM: Magnetic Field Cancellation Systems
13, 26 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
13, 26 -- To: Roseann Csencsits {RCsencsits-at-lbl.gov} , {Microscopy-at-Microscopy.Com}
13, 26 -- CC: {david.mitchell-at-emu.usyd.edu.au}
13, 26 -- Message-ID: {C625BA1D.9EF%david.mitchell-at-emu.usyd.edu.au}
13, 26 -- Thread-Topic: [Microscopy] viaWWW: TEM: Magnetic Field Cancellation Systems
13, 26 -- Thread-Index: AcnNEb5x/MdHrjkEEd62AAAjMrpsqg==
13, 26 -- In-Reply-To: {AB9D0E24-CA2B-4E39-8BB3-0364B32A95B1-at-lbl.gov}
13, 26 -- Mime-version: 1.0
13, 26 -- Content-type: text/plain;
13, 26 -- charset="US-ASCII"
13, 26 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: camiller-at-anatomy.iupui.edu
Date: Mon, 4 May 2009 18:53:12 -0500
Subject: [Microscopy] viaWWW: Old EM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both camiller-at-anatomy.iupui.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: camiller-at-anatomy.iupui.edu
Name: Caroline Miller

Organization: Indiana University

Title-Subject: [Filtered] Old EM film

Question: I have several boxes (100 sheets) of Kodak, 31/4 x 4in.
4489 EM film. It is quite old, 1988, but has been kept in the
refrigerator. Will give away if you pay for shipping.

Login Host: 134.68.251.124
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 4 18:53:11 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44NrBZi031216
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 18:53:11 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c6253055c905-at-[206.69.208.22]}
6, 11 -- Date: Mon, 4 May 2009 18:53:10 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: camiller-at-anatomy.iupui.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Old EM film
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: reznik-at-ict.uni-karlsruhe.de
Date: Mon, 4 May 2009 18:53:39 -0500
Subject: [Microscopy] viaWWW: Aperture_Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both reznik-at-ict.uni-karlsruhe.de as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: reznik-at-ict.uni-karlsruhe.de
Name: Reznik

Organization: University

Title-Subject: [Filtered] Aperture_Cleaning

Question: Hello,
I need to clean the objective Mo-aperture of SEM S570-Hitachi.
Does anybody know a simple method for that?
Boris

Login Host: 129.13.186.4
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 4 18:53:39 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44NrbRY031804
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 18:53:38 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6253072cfdb-at-[206.69.208.22]}
6, 11 -- Date: Mon, 4 May 2009 18:53:37 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: reznik-at-ict.uni-karlsruhe.de (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Aperture_Cleaning
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Mon, 4 May 2009 19:02:34 -0500
Subject: [Microscopy] Re: Need 40 mm diameter metallurgical mount holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe:

A simple solution: buy one of the 38 mm holders
from Pella, and have the inside diameter machined
out to 40.5 mm - I believe the wall thickness is
enough to accommodate doing this,

Wil Bigelow
=============================


} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731


==============================Original Headers==============================
8, 20 -- From bigelow-at-umich.edu Mon May 4 19:02:34 2009
8, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4502YBH027239
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 19:02:34 -0500
8, 20 -- Received: FROM [99.130.70.208] (adsl-99-130-70-208.dsl.sfldmi.sbcglobal.net [99.130.70.208])
8, 20 -- By hellskitchen.mr.itd.umich.edu ID 49FF8218.A7B50.29524 ;
8, 20 -- Authuser bigelow;
8, 20 -- 4 May 2009 20:02:32 EDT
8, 20 -- Mime-Version: 1.0
8, 20 -- Message-Id: {p06240801c62531a356b0-at-[99.130.70.208]}
8, 20 -- In-Reply-To: {200905041939.n44JdJEJ015836-at-ns.microscopy.com}
8, 20 -- References: {200905041939.n44JdJEJ015836-at-ns.microscopy.com}
8, 20 -- Date: Mon, 4 May 2009 20:02:27 -0400
8, 20 -- To: kintzjd-at-rocketmail.com, Microscopy Listserver {microscopy-at-microscopy.com}
8, 20 -- From: Wil Bigelow {bigelow-at-umich.edu}
8, 20 -- Subject: Re: [Microscopy] Need 40 mm diameter metallurgical mount holder
8, 20 -- for SEM
8, 20 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 20 -- Content-Transfer-Encoding: 8bit
8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4502YBH027239
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Mon, 4 May 2009 20:11:59 -0500
Subject: [Microscopy] fluxless rock bead fusion method?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Is anyone out there using a metal strip or graphite method to fuse small
(maybe 100mg or so) amounts of rock powder without flux into a glassy bead
which can thenbe mounted, cut, and polished for EPMA or LA-ICP-MS analysis?

I'm hoping to convert my (t)rusty old Edwards 306 vacuum coater into such
a device but I'm trying to avoid reinventing too many wheels.

I remember seeing such a method setup at UC Davis about ten years ago but
I haven't had any luck following that one up.

We actually want to use LA-ICP-MS so it seems to us that a graphite boat
setup would be better from a contamination viewpoint than a metal strip.

Any references, tips, experiences etc would be most welcome.

cheers
Ritchie Sims
Geology
Auckland University
Auckland
New Zealand



==============================Original Headers==============================
9, 28 -- From r.sims-at-auckland.ac.nz Mon May 4 20:11:59 2009
9, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n451BwFF014482
9, 28 -- for {microscopy-at-Microscopy.Com} ; Mon, 4 May 2009 20:11:58 -0500
9, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3FED29D427
9, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 13:11:58 +1200 (NZST)
9, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
9, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
9, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
9, 28 -- with ESMTP id Wo6IE5CZAqg5 for {microscopy-at-Microscopy.Com} ;
9, 28 -- Tue, 5 May 2009 13:11:58 +1200 (NZST)
9, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
9, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
9, 28 -- (No client certificate requested)
9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1C1829D426
9, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 13:11:58 +1200 (NZST)
9, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
9, 28 -- To: microscopy-at-Microscopy.Com
9, 28 -- Date: Tue, 05 May 2009 13:14:31 +1200
9, 28 -- MIME-Version: 1.0
9, 28 -- Subject: fluxless rock bead fusion method?
9, 28 -- Message-ID: {4A003BB7.4502.14AFD56-at-r.sims.auckland.ac.nz}
9, 28 -- Priority: normal
9, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
9, 28 -- Content-type: text/plain; charset=US-ASCII
9, 28 -- Content-transfer-encoding: 7BIT
9, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: Lubomir.Kovacik-at-lf1.cuni.cz
Date: Tue, 5 May 2009 07:47:35 -0500
Subject: [Microscopy] viaWWW: broken pin on Gatan 626 cryoholder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Lubomir.Kovacik-at-lf1.cuni.cz as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Lubomir.Kovacik-at-lf1.cuni.cz
Name: Lubomir Kovacik

Organization: Institute of cellular biology and pathology, 1st fac
med, Charles Uni, Prague

Title-Subject: [Filtered] broken pin on Gatan 626 cryoholder

Question: Dear fellow microscopists,

do you know if a broken stage-positioning pin on the Gatan 626
cryoholder can be replaced without having to send it to Gatan? The
pin seems to be just "pressed" into the bar, so maybe careful
drilling....Unfortunately we lost the broken part of the pin.

Thanks for any response,

Lubomir

Login Host: 195.113.48.5
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue May 5 07:47:35 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45ClXPU025525
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 07:47:34 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c625e5d052a5-at-[206.69.208.22]}
9, 11 -- Date: Tue, 5 May 2009 07:47:32 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: Lubomir.Kovacik-at-lf1.cuni.cz (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: broken pin on Gatan 626 cryoholder
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jd-at-laddresearch.com
Date: Tue, 5 May 2009 14:43:22 -0500
Subject: [Microscopy] Re: viaWWW: Aperture_Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Boris,

Moly apertures will oxidize if not cleaned in an evaporator. With PT
you can flame them, which is an advantage. MO tends to be more
durable than PT which is an advantage of MO.

We use special cleaning methods for MO apertures during production
but it's cost prohibitive to use these techniques unless you're
making and producing many moly and platinum apertures each day.

I would suggest you try cleaning the strip in acetone in an
ultrasonic which might help a little, but without access to an
evaporator you won't get really good cleaning.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

Disclaimer: Ladd Research is in the business of producing and
selling apertures for use in electron microscopes and other applications.

At 07:59 PM 5/4/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
15, 27 -- From jd-at-laddresearch.com Tue May 5 14:43:22 2009
15, 27 -- Received: from anders.electric.net (anders.electric.net [72.35.23.15])
15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45JhMRB031005
15, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 14:43:22 -0500
15, 27 -- Received: from 1M1QXx-0001Oj-UQ by anders.electric.net with emc1-ok (Exim 4.69)
15, 27 -- (envelope-from {jd-at-laddresearch.com} )
15, 27 -- id 1M1QXx-0001Pd-Vb; Tue, 05 May 2009 12:43:21 -0700
15, 27 -- Received: by emcmailer; Tue, 05 May 2009 12:43:21 -0700
15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com)
15, 27 -- by anders.electric.net with esmtps (TLSv1:AES256-SHA:256)
15, 27 -- (Exim 4.69)
15, 27 -- (envelope-from {jd-at-laddresearch.com} )
15, 27 -- id 1M1QXx-0001Oj-UQ; Tue, 05 May 2009 12:43:21 -0700
15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
15, 27 -- Date: Tue, 05 May 2009 15:43:18 -0400
15, 27 -- To: reznik-at-ict.uni-karlsruhe.de
15, 27 -- From: jd {jd-at-laddresearch.com}
15, 27 -- Subject: Re: [Microscopy] viaWWW: Aperture_Cleaning
15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com}
15, 27 -- In-Reply-To: {200905042359.n44Nx3XC016254-at-ns.microscopy.com}
15, 27 -- References: {200905042359.n44Nx3XC016254-at-ns.microscopy.com}
15, 27 -- Mime-Version: 1.0
15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
15, 27 -- X-Outbound-IP: 216.204.198.170
15, 27 -- X-Env-From: jd-at-laddresearch.com
15, 27 -- X-Virus-Status: Scanned by VirusSMART (c)
15, 27 -- Message-Id: {E1M1QXx-0001Pd-Vb-at-anders.electric.net}
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 5 May 2009 17:51:11 -0500
Subject: [Microscopy] viaWWW: Aperture_Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Boris,
A very simple way to clean almost all types of apertures (except thin foil
gold self cleaning) is to polish them with 1µ diamond paste.

I prefer a cut-knap polishing cloth, but even a woven lint-free cloth will
do. The 1µ diamond compound should be silicone-free (SPI meets this
requirement, according to the late Chuck Garber, although many or most
metallographic pastes have silicones). Place some diamond compound on the
polishing cloth, place the aperture (or aperture strip) on the diamond, put
your finger on it and start moving it in circles. When the first side is
clean, flip it over and polish the other side. Ultrasonicate in hot water
and Joy dishwashing liquid, rinse (tap water will suffice, although
distilled is better) and blow dry with a duster or very clean compressed
air, so there are no water spots.

Apertures can last decades. I've even taken apertures that had been
overheated in an evaporator and didn't work, and made them whole again.
Evaporator cleaning causes the metal to change its crystal structure and
often after about 5-6 cleanings, the aperture is no good any more.
Polishing will smear any distinct grain boundaries and make the aperture
good again.

Have fun.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: reznik-at-ict.uni-karlsruhe.de [mailto:reznik-at-ict.uni-karlsruhe.de]
Sent: Monday, May 04, 2009 7:56 PM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both reznik-at-ict.uni-karlsruhe.de as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: reznik-at-ict.uni-karlsruhe.de
Name: Reznik

Organization: University

Title-Subject: [Filtered] Aperture_Cleaning

Question: Hello,
I need to clean the objective Mo-aperture of SEM S570-Hitachi.
Does anybody know a simple method for that?
Boris

Login Host: 129.13.186.4
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 4 18:53:39 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n44NrbRY031804
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 18:53:38
-0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6253072cfdb-at-[206.69.208.22]}
6, 11 -- Date: Mon, 4 May 2009 18:53:37 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: reznik-at-ict.uni-karlsruhe.de (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Aperture_Cleaning
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
21, 26 -- From kenconverse-at-qualityimages.biz Tue May 5 17:51:11 2009
21, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
21, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45MpA7X021269
21, 26 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 17:51:11 -0500
21, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
21, 26 -- with ESMTP
21, 26 -- id {20090505225110481.TMGQ17490-at-cdptpa-omta04.mail.rr.com} ;
21, 26 -- Tue, 5 May 2009 22:51:10 +0000
21, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 26 -- To: {reznik-at-ict.uni-karlsruhe.de} ,
21, 26 -- "MSA Listserver" {microscopy-at-microscopy.com}
21, 26 -- Subject: RE: [Microscopy] viaWWW: Aperture_Cleaning
21, 26 -- Date: Tue, 5 May 2009 18:51:04 -0400
21, 26 -- Message-ID: {B67DCC1EB786453E8B6D1821208AC1A9-at-Ken}
21, 26 -- MIME-Version: 1.0
21, 26 -- Content-Type: text/plain;
21, 26 -- charset="iso-8859-1"
21, 26 -- X-Priority: 3 (Normal)
21, 26 -- X-MSMail-Priority: Normal
21, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 26 -- In-Reply-To: {200905042356.n44NuE7p006839-at-ns.microscopy.com}
21, 26 -- Importance: Normal
21, 26 -- Thread-Index: AcnNE+/rsb7WIeAtSt+2Hx7ZUB+qlQAvYf7w
21, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 26 -- Content-Transfer-Encoding: 8bit
21, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n45MpA7X021269
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 5 May 2009 18:19:46 -0500
Subject: [Microscopy] viaWWW: Aperture_Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


what about using Pol? then, rinse with IPA and then
methanol. I've done this for Wenhelts but not for apertures.
I just replaced them with new Pt discs but have not had
an aperture strip.

The aperture holder also got Pol and cleaning. The blue
cast is cleaned off and stig returns to normal.

gary g.


At 03:52 PM 5/5/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
11, 22 -- From gary-at-gaugler.com Tue May 5 18:19:46 2009
11, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n45NJjMI003835
11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 18:19:46 -0500
11, 22 -- Message-Id: {200905052319.n45NJjMI003835-at-ns.microscopy.com}
11, 22 -- Received: (qmail 25367 invoked from network); 5 May 2009 16:32:35 -0700
11, 22 -- Received: by simscan 1.1.0 ppid: 25361, pid: 25365, t: 0.1482s
11, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 22 -- by smtp1 with SMTP; 5 May 2009 16:32:35 -0700
11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 22 -- Date: Tue, 05 May 2009 16:19:43 -0700
11, 22 -- To: kenconverse-at-qualityimages.biz
11, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 22 -- Subject: Re: [Microscopy] RE: viaWWW: Aperture_Cleaning
11, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 22 -- In-Reply-To: {200905052252.n45Mqtlt023694-at-ns.microscopy.com}
11, 22 -- References: {200905052252.n45Mqtlt023694-at-ns.microscopy.com}
11, 22 -- Mime-Version: 1.0
11, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
11, 22 -- Content-Transfer-Encoding: 8bit
11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n45NJjMI003835
==============================End of - Headers==============================




From: p.czerny-at-usyd.edu.au
Date: Tue, 5 May 2009 18:23:19 -0500
Subject: [Microscopy] Biological Specimen Preparation Specialist/Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Biological Specimen Preparation Specialist/Microscopist
Australian Key Centre for Microscopy and Microanalysis
The University of Sydney
Reference No. 154769

The Australian Key Centre for Microscopy & Microanalysis (AKCMM) is the
headquarters of the Australian Microscopy and Microanalysis Research
Facility (AMMRF), a major collaboration between government and universities.
The Centre recognises the substantial role that microscopy, imaging and
microanalysis are set to play in the next decade, as biotechnology and
nanoscience have increasing impact on science and society. The Centre
provides leadership, innovation and ingenuity in Australian science and
engineering research. For more information, please visit www.emu.usyd.edu.au

The AKCMM seeks to appoint a highly-organised and self-driven individual to
manage and operate Biological Specimen Preparation facilities and
laboratory, and train and assist users with microscopy techniques and
specimen imaging. You will have the opportunity to support a wide range of
cutting-edge research, on our suite of state-of-the-art microscopy
equipment. This position will see you provide training and assistance in
various specimen preparation techniques, including but not be limited to
chemical and cryo fixation. So expertise in these techniques is desirable.
You will also be required to demonstrate microscopy techniques such as SEM,
TEM, Confocal, and fluorescence microscopy, plus IT skills including
databases and image and data processing software. Competency in some of
these areas is required. Occasional national/international travel is also
required.

An important part of the role is contribution towards preparation and
delivery of training courses, coursework programs and workshops, and
ensuring a productive and pleasant learning and working environment for all
users. Therefore you must also be able to demonstrate strong interpersonal
and communication skills. You will be an organised person who manages your
priorities well. As this position also requires you to maintain
documentation, manuals and instrument logs, as well as report usage trends
and user/specimen information to management. You will also be responsible on
ensuring safe equipment operation and the availability of adequate
laboratory and instrument consumables.

To succeed, you will possess a relevant degree and a background in biology,
biochemistry, chemistry, chemical or mechanical engineering, materials
science or engineering, medicine, nanotechnology, pharmacy or physics.

If you are passionate about sharing your microscopy knowledge and supporting
research, this is the job for you. The position is full-time fixed term for
three years, subject to the completion of a satisfactory probation period
for new appointees. Visa sponsorship and relocation assistance may be
offered to suitable overseas applicants. A wide range of employment benefits
are also available, including Living Away From Home Allowance (LAFHA).

Level of appointment and responsibility will be commensurate with
qualifications and experience:

Remuneration package Level 5: $63k - $70k p.a. (which includes base salary
$52k - $59k p.a., leave loading and 17% employer's contribution to
superannuation).

Remuneration package Level 6: $72k - $78k p.a. (which includes base salary
$61k - $66k p.a., leave loading and 17% employer's contribution to
superannuation).

For more information and to apply, please visit http://positions.edu.edu.au
and search by reference number 154769.

Specific enquiries about the role can be directed to Ellie Kable on (02)
9351 7566.

Closing Date: 21 May 2009



Kind regards,

Paulina Czerny
Sydney Recruitment
University of Sydney
p.czerny-at-usyd.edu.au




==============================Original Headers==============================
18, 17 -- From p.czerny-at-usyd.edu.au Tue May 5 18:23:19 2009
18, 17 -- Received: from hrx.com.au (210-193-133-2.macquarie.net.au [210.193.133.2] (may be forged))
18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45NNHaA011369
18, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 18:23:18 -0500
18, 17 -- Received: from HRXSYD022 ([192.168.15.152]) by mail.hrx.com.au with InterScan Message Security Suite; Wed, 06 May 2009 09:26:47 +1000
18, 17 -- From: "Paulina Czerny" {p.czerny-at-usyd.edu.au}
18, 17 -- To: {Microscopy-at-Microscopy.Com}
18, 17 -- Subject: Biological Specimen Preparation Specialist/Microscopist
18, 17 -- Date: Wed, 6 May 2009 09:23:13 +1000
18, 17 -- Message-ID: {001001c9cdd8$75f859d0$980fa8c0-at-hrx.com.au}
18, 17 -- MIME-Version: 1.0
18, 17 -- Content-Type: text/plain;
18, 17 -- charset="us-ascii"
18, 17 -- Content-Transfer-Encoding: 7bit
18, 17 -- X-Mailer: Microsoft Office Outlook 11
18, 17 -- Thread-Index: AcnN2HXLpdEWa+tVQji2xZqZVcfJUw==
18, 17 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198
==============================End of - Headers==============================




From: p.czerny-at-usyd.edu.au
Date: Tue, 5 May 2009 18:25:14 -0500
Subject: [Microscopy] Careers in Microscopy and Microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Careers in Microscopy and Microanalysis
Expressions of Interest in Technical and Research Opportunities
Reference No. 154930

The Australian Key Centre for Microscopy & Microanalysis (AKCMM), located in
Camperdown, on the main campus of the University of Sydney, was established
as the Electron Microscope Unit in 1958 and is a centralised corporate unit
of the University. The Centre recognises the substantial role that
microscopy, imaging and microanalysis are set to play in the next decade, as
biotechnology and nanoscience have increasing impact on science and society.
The Centre provides leadership, innovation and ingenuity in Australian
science and engineering research and is seeking to recruit outstanding
academic and general staff to advance this aim.

The Centre is a research leader and has some of the most advanced facilities
in the world for microscopy and microanalysis. It serves as national
headquarters for the Australian Microscopy and Microanalysis Research
Facility (AMMRF) (www.ammrf.org.au). The Centre supports a diverse user base
to develop techniques to enable understanding of the relationships between
the structure and function of materials and living systems. For details see:
www.emu.usyd.edu.au

There will soon be employment opportunities in the Centre in the form of
technical staff positions and research staff positions. The positions are
available at various levels and in various discipline areas. The principal
role of the Centre's technical staff is to provide user-support. This takes
the form variously of user-training: both one-on-one and in groups and via
the Centre's coursework programs. User-support may also involve instrument
and/or laboratory coordination and requires technical mastery of particular
microscopy platforms. Direct work with users to help and enable them to make
the most from the superb instrumentation in this outstanding Centre is a key
job-function. We welcome Expressions of Interest (EoI) from scientists and
engineers with interest, knowledge and expertise in TEM, SEM, FIB,
light/laser microscopy and the microanalysis variants of these platforms.

Alternatively, our research staff positions are for early career researchers
seeking postdoctoral research fellowship roles. Currently, the emerging
positions in the Centre are grant-supported and mainly in the area of
materials science and engineering. Passionate researchers with a materials
science or relevant Ph.D. that have interest and knowledge in phase
transformations and structure-property relationships and expertise in
advanced electron microscopy and related techniques are encouraged to submit
an EoI.

For more information and to apply, please visit http://positions.usyd.edu.au
and search by reference number 154930.

Specific enquiries can be directed to Centre Manager Karen Hill via
karen.hill-at-emu.usyd.edu.au or +61 2 9351 4499.

Close Date: 24 May 2009


Kind regards,

Paulina Czerny
Sydney Recruitment
University of Sydney
p.czerny-at-usyd.edu.au





==============================Original Headers==============================
15, 17 -- From p.czerny-at-usyd.edu.au Tue May 5 18:25:14 2009
15, 17 -- Received: from hrx.com.au (210-193-133-2.macquarie.net.au [210.193.133.2] (may be forged))
15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45NPDEK017397
15, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 18:25:13 -0500
15, 17 -- Received: from HRXSYD022 ([192.168.15.152]) by mail.hrx.com.au with InterScan Message Security Suite; Wed, 06 May 2009 09:28:43 +1000
15, 17 -- From: "Paulina Czerny" {p.czerny-at-usyd.edu.au}
15, 17 -- To: {Microscopy-at-Microscopy.Com}
15, 17 -- Subject: Careers in Microscopy and Microanalysis
15, 17 -- Date: Wed, 6 May 2009 09:25:08 +1000
15, 17 -- Message-ID: {001101c9cdd8$baf83af0$980fa8c0-at-hrx.com.au}
15, 17 -- MIME-Version: 1.0
15, 17 -- Content-Type: text/plain;
15, 17 -- charset="us-ascii"
15, 17 -- Content-Transfer-Encoding: 7bit
15, 17 -- X-Mailer: Microsoft Office Outlook 11
15, 17 -- Thread-Index: AcnN2Lq1BmIixakdSbCHjUpo95+/wg==
15, 17 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198
==============================End of - Headers==============================




From: RWNordhausen-at-UCDavis.edu
Date: Tue, 5 May 2009 21:15:29 -0500
Subject: [Microscopy] viaWWW: TEM morphodiagnostic job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both RWNordhausen-at-UCDavis.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: RWNordhausen-at-UCDavis.edu
Name: Robert W. Nordhausen

Organization: California Animal Health and Food Safety Lab

Title-Subject: [Filtered] TEM morphodiagnostic job opening

Question: Microscopists,
Stand-alone TEM electron microcopist, thin section and negative stain
morphodiagnostics in a food animal veterinary diagnostic lab.
Experienced in ultrastructural pathology. PhD preferred.
Requisition #0300622.
https://www.employment.ucdavis.edu
Thanks,
Bob Nordhausen
EM Lab
California Animal Health and Food Safety Lab
School of Veterinary Medicine
University of California, Davis

Login Host: 169.237.116.251
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue May 5 21:15:28 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n462FRGH019307
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 21:15:28 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c626a326b3a8-at-[206.69.208.22]}
6, 11 -- Date: Tue, 5 May 2009 21:15:26 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: RWNordhausen-at-UCDavis.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM morphodiagnostic job opening
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zhangyc-at-gmail.com
Date: Tue, 5 May 2009 21:16:10 -0500
Subject: [Microscopy] viaWWW: CBEM pattern refining program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zhangyc-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: zhangyc-at-gmail.com
Name: Yucheng Zhang

Organization: university of cambridge

Title-Subject: [Filtered] CBEM pattern refining program

Question: I would like to do some nano-scale strain measurement using
CBED. In order to retrieve the precise lattice parameters,
experimental pattern is going to be compared with simulated pattern
obtained using JEMS. I learn that a kai-square minimization process
can be used to compare the experiemental data with simulation. Can
anyone kindly suggest any free programs I can download to do the
kai-square miminization for this purpose?
Many thanks

Login Host: 86.22.78.237
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue May 5 21:16:10 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n462G8lS019785
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 21:16:10 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240805c626a348bbb6-at-[206.69.208.22]}
6, 11 -- Date: Tue, 5 May 2009 21:16:07 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: zhangyc-at-gmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: CBEM pattern refining program
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mmcheath-at-syr.edu
Date: Wed, 6 May 2009 06:52:33 -0500
Subject: [Microscopy] Field Emission SEM's: applications in the field of Geology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am seeking input regarding the usefulness/application of Field Emission
SEM's in the field of Geology. If you are using one for geological
applications, are you sing it for SEI imaging and/or Backscatter imaging?
How about Cathodoluminescence? EDS? EBSD?

TIA
Mike

********************************************************************
Michael M. Cheatham
312 Heroy Geology Laboratory Phone (315)-443-1261
Syracuse University Fax (315)-443-3363
Syracuse, NY 13244-1070

email:mmcheath-at-syr.edu
http://earthsciences.syr.edu

owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html
owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html
owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html
owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html
********************************************************************



==============================Original Headers==============================
8, 28 -- From mmcheath-at-syr.edu Wed May 6 06:52:33 2009
8, 28 -- Received: from mx1.syr.edu (mx1.syr.edu [128.230.1.71])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46BqWsh013987
8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 06:52:33 -0500
8, 28 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195])
8, 28 -- by mx1.syr.edu (8.14.3/8.14.3) with ESMTP id n46BqVoh021699
8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 07:52:31 -0400
8, 28 -- Received: from suex07-mbx-02.ad.syr.edu ([fe80::2403:4941:8a06:15cc]) by
8, 28 -- suex07-hub-01.ad.syr.edu ([2002:80e6:6cc3::80e6:6cc3]) with mapi; Wed, 6 May
8, 28 -- 2009 07:52:31 -0400
8, 28 -- From: Michael M Cheatham {mmcheath-at-syr.edu}
8, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
8, 28 -- Date: Wed, 6 May 2009 07:52:30 -0400
8, 28 -- Subject: Field Emission SEM's: applications in the field of Geology
8, 28 -- Thread-Topic: Field Emission SEM's: applications in the field of Geology
8, 28 -- Thread-Index: AcnOQSJG2dnijbbIIEC8wI9U0F2AmA==
8, 28 -- Message-ID: {C626F23E.2D8B7%mmcheath-at-syr.edu}
8, 28 -- Accept-Language: en, en-US
8, 28 -- Content-Language: en
8, 28 -- X-MS-Has-Attach:
8, 28 -- X-MS-TNEF-Correlator:
8, 28 -- acceptlanguage: en, en-US
8, 28 -- Content-Type: text/plain; charset="iso-8859-1"
8, 28 -- MIME-Version: 1.0
8, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-05-06_06:2009-04-28,2009-05-06,2009-05-06 signatures=0
8, 28 -- X-Proofpoint-Spam-Reason: safe
8, 28 -- Content-Transfer-Encoding: 8bit
8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n46BqWsh013987
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Wed, 6 May 2009 11:11:11 -0500
Subject: [Microscopy] Flat sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Highly hygroscopic tissues swell when sections are floated on water, and
when they dry down to the slide, they have folds because they have stretched
more than the surrounding tissue. I have tried floating the sections on
some organic solvents instead of water, but the sections sink and end up
even more wrinkled. Has anyone worked out a solution to this problem? I
often have it with both "Epon" and ImmunoBed sections.

Ralph Common
Michigan State University


==============================Original Headers==============================
4, 24 -- From rcommon-at-msu.edu Wed May 6 11:11:11 2009
4, 24 -- Received: from sys06.mail.msu.edu (sys06.mail.msu.edu [35.9.75.106])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46GBB76009619
4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 6 May 2009 11:11:11 -0500
4, 24 -- Received: from [35.9.122.125] (helo=emlab)
4, 24 -- by sys06.mail.msu.edu with esmtpsa (Exim 4.63 #12)
4, 24 -- (TLSv1:RC4-MD5:128)
4, 24 -- id 1M1jiA-00024E-ON
4, 24 -- for Microscopy-at-microscopy.com; Wed, 06 May 2009 12:11:10 -0400
4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
4, 24 -- To: {Microscopy-at-microscopy.com}
4, 24 -- Subject: Flat sections
4, 24 -- Date: Wed, 6 May 2009 13:10:46 -0400
4, 24 -- Message-ID: {000d01c9ce6d$9972bb40$7d7a0923-at-msu.edu}
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: text/plain;
4, 24 -- charset="iso-8859-1"
4, 24 -- Content-Transfer-Encoding: 7bit
4, 24 -- X-Priority: 3 (Normal)
4, 24 -- X-MSMail-Priority: Normal
4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
4, 24 -- Importance: Normal
4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
4, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Wed, 6 May 2009 11:32:03 -0500
Subject: [Microscopy] Re: Field Emission SEM's: applications in the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michael,
Our CAMCOR nano-characterization facility is multi-disciplinary
however, the geologists here at UofO find our suite of FEG SEMs
(Zeiss Ultra 55, FEI Helios FIB and Quanta) extremely useful for all
sorts of applications that hadn't imagined at the time of the
instrument purchases.

For example, geologists are using a Quanta SEM to examine nano-scale
decompression crystallization in eruptive materials and finding a
host of interesting processes using both SE, BSE and even
cathodoluminescence. In a related field, archeologists are taking
advantage of our Titan TEM to characterize nano-diamonds from the
Younger Dryas ice-age impact layer. Whoever heard of archeologists
using a TEM, but taking advantage of new "eyes" is partly how new
discoveries are made in science.

The question almost seems unnecessary as the geologists don't seem to
stop their investigations at 50 or 100Kx any more than any other
field. There certainly are useful things that can continue to be done
at lower magnifications, but the capability for looking closer is
always welcome and we wouldn't go back to tungsten filaments if I can help it.

The question is somewhat related to asking why would someone put a
FEG on a microprobe or ESEM. My response would be, that while one can
come up with a number of specialized applications that actually do
benefit from performing x-ray analyses or low vacuum imaging on
sub-micron domains, my basic answer is that it is simply a
wonderfully powerful ability to be able to drop into low current or
high vacuum mode and acquire a high resolution SE image with a click
of the mouse without moving to another instrument.

That said, it is true that CL and EBSD tend to have inherently larger
interaction volumes than SE or straight up BSE, but still the smaller
spot sizes of a FEG don't hurt in these specific cases.

Another big advantage for FEG electron instruments is that they can
operate with more stability at lower voltages than instruments with
tungsten filaments sources. Being able to easily work below 5 keV has
all sorts of analytical and imaging advantages for geologists as you
might imagine.
john

At 04:58 AM 5/6/2009, mmcheath-at-syr.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 21 -- From donovan-at-uoregon.edu Wed May 6 11:32:03 2009
11, 21 -- Received: from QMTA12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46GW2g3024565
11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 11:32:02 -0500
11, 21 -- Message-Id: {200905061632.n46GW2g3024565-at-ns.microscopy.com}
11, 21 -- Received: from OMTA01.emeryville.ca.mail.comcast.net ([76.96.30.11])
11, 21 -- by QMTA12.emeryville.ca.mail.comcast.net with comcast
11, 21 -- id oBdc1b0020EPchoACGY3Vf; Wed, 06 May 2009 16:32:03 +0000
11, 21 -- Received: from SOURCE.uoregon.edu ([71.237.220.159])
11, 21 -- by OMTA01.emeryville.ca.mail.comcast.net with comcast
11, 21 -- id oGY21b00Q3SwvZT8MGY22u; Wed, 06 May 2009 16:32:03 +0000
11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 21 -- Date: Wed, 06 May 2009 09:32:00 -0700
11, 21 -- To: microscopy-at-microscopy.com
11, 21 -- From: "John J. Donovan" {donovan-at-uoregon.edu}
11, 21 -- Subject: Re: [Microscopy] Field Emission SEM's: applications in the
11, 21 -- field of Geology
11, 21 -- In-Reply-To: {200905061158.n46BwddQ022036-at-ns.microscopy.com}
11, 21 -- References: {200905061158.n46BwddQ022036-at-ns.microscopy.com}
11, 21 -- Mime-Version: 1.0
11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Wed, 6 May 2009 12:42:36 -0500
Subject: [Microscopy] Re: Flat sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On May 6, 2009, at 9:11 AM, rcommon-at-msu.edu wrote:

} Highly hygroscopic tissues swell when sections are floated on water,
} and
} when they dry down to the slide, they have folds because they have
} stretched
} more than the surrounding tissue. I have tried floating the
} sections on
} some organic solvents instead of water, but the sections sink and
} end up
} even more wrinkled. Has anyone worked out a solution to this
} problem? I
} often have it with both "Epon" and ImmunoBed sections.


Dear Ralph,
I have had no experience with this problem, so I don't know if this
will work, but the sections should not sink in solvents that are
heavier than water and have surface tension greater than water. I
think there are several such solvents; I know the chlorinated ones--
CCl4, CHCl3, etc--are heavier than water, but I don't know what their
surface tensions are, and I do not know whether they will damage the
sections, since they are pretty active solvents. I think that n-
butanol might have sufficiently high surface tension, and it would
definitely be gentler on the sections, but it may not have sufficient
density. Possibly some mixture could be found that has all the right
properties. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Wed May 6 12:42:35 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46HgZ7f009641
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 12:42:35 -0500
6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 5738766E16A3
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 10:42:35 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id A059066E4185
6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 10:42:33 -0700 (PDT)
6, 22 -- Message-Id: {61649146-36EE-4141-BFB6-4030173AD2FD-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200905061611.n46GBKtm009745-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Flat sections
6, 22 -- Date: Wed, 6 May 2009 10:42:33 -0700
6, 22 -- References: {200905061611.n46GBKtm009745-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Wed, 6 May 2009 12:46:15 -0500
Subject: [Microscopy] Flat sections (again)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for those who responded to my original post. I was not specific
enough about the problem, and how I normally handle semithin sections. I
float the 1 micron sections on drops of water on slides, and heat the slides
on a hotplate until they have dried down. For most tissues I get perfectly
flat sections that adhere well. The problem is differential stretching of
the section, with some areas stretching more than the surrounding areas of
the section. This is a problem particularly with lichen and other
ascomycete fruiting bodies. Most of the section lies flat, but the
hymenium, the area of most interest, has multiple folds. The folds are
present even before staining. The hymenium contains gel-like
polysaccharides that absorb large amounts of water, causing excessive
swelling and stretching of the hymenial areas relative to the surrounding
tissue, and thus folds in the hymenium when the sections dry down. My
thought was to float the sections on an organic solvent instead of water,
but the sections just sink in the solvents I have tried so far. Has anyone
had success floating sections on something other than water? Any other cure
for the problem? Cryosectioning avoids the problem, but I would like to be
able to get good sections with embedded specimens.

Ralph Common
Michigan State University


==============================Original Headers==============================
3, 24 -- From rcommon-at-msu.edu Wed May 6 12:46:13 2009
3, 24 -- Received: from sys03.mail.msu.edu (sys03.mail.msu.edu [35.9.75.103])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46HkBCw014387
3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 6 May 2009 12:46:12 -0500
3, 24 -- Received: from [35.9.122.125] (helo=emlab)
3, 24 -- by sys03.mail.msu.edu with esmtpsa (Exim 4.63 #12)
3, 24 -- (TLSv1:RC4-MD5:128)
3, 24 -- id 1M1lC7-000474-Nz
3, 24 -- for Microscopy-at-microscopy.com; Wed, 06 May 2009 13:46:11 -0400
3, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
3, 24 -- To: {Microscopy-at-microscopy.com}
3, 24 -- Subject: Flat sections (again)
3, 24 -- Date: Wed, 6 May 2009 14:45:47 -0400
3, 24 -- Message-ID: {001401c9ce7a$df7b46e0$7d7a0923-at-msu.edu}
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- charset="iso-8859-1"
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- X-Priority: 3 (Normal)
3, 24 -- X-MSMail-Priority: Normal
3, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
3, 24 -- Importance: Normal
3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
3, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 6 May 2009 19:52:43 -0500
Subject: [Microscopy] Hitachi S-570

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Group:

I've dropped the S-5700 in favor of the older S-570
as a general purpose SEM that is low cost of maintenance.

Can any of you tell me about the good, the bad, the
ugly side of this system? It can have a turbo or
DP. I am only looking for LaB6 capability. Depending
on what EDS it might have, digital capture is possible.
Otherwise, I would look at Quartz PCI. At some point,
I would put an EDAX Apollo 10 on this system.

I know very little about Hitachi and JEOL but much
about Amray and Zeiss. Thus, I seek input from the
collective.

Off-line is OK.

gary g.


==============================Original Headers==============================
7, 17 -- From gary-at-gaugler.com Wed May 6 19:52:43 2009
7, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n470qh99026708
7, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 19:52:43 -0500
7, 17 -- Message-Id: {200905070052.n470qh99026708-at-ns.microscopy.com}
7, 17 -- Received: (qmail 10200 invoked from network); 6 May 2009 18:05:36 -0700
7, 17 -- Received: by simscan 1.1.0 ppid: 10197, pid: 10198, t: 0.1400s
7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
7, 17 -- by smtp1 with SMTP; 6 May 2009 18:05:36 -0700
7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
7, 17 -- Date: Wed, 06 May 2009 17:52:35 -0700
7, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
7, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
7, 17 -- Subject: Hitachi S-570
7, 17 -- Mime-Version: 1.0
7, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Thu, 7 May 2009 01:27:45 -0500
Subject: [Microscopy] Manuals for Reichart/Leica KF80 and AFS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I recently discovered (in storage) Reichart/Leica KF80 Plunge Freezer and AFS (Automatic Freeze Substitution) systems. I would like to get these up and running but the manuals have vanished into the mists of time.

I have tried to find the manuals on the 'net with no success (yet).

If anyone has copies (electronic preferably) they could send it would be very much appreciated.

Thank you very much.

Colin Veitch
Electron Microscopist
CSIRO Materials Science and Engineering, Geelong Laboratory
PO Box 21, BELMONT, Vic. 3216. Australia.
colin.veitch-at-csiro.au

Tel: +61 (0) 3 5246 4000  
Mobile: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.




==============================Original Headers==============================
11, 46 -- From prvs=371dffb59=Colin.Veitch-at-csiro.au Thu May 7 01:27:44 2009
11, 46 -- Received: from act-MTAout4.csiro.au (act-MTAout4.csiro.au [150.229.7.41])
11, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n476Rhbp027048
11, 46 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 01:27:44 -0500
11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
11, 46 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt;
11, 46 -- s=email; t=1241677664; x=1273213664;
11, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
11, 46 -- mime-version:content-transfer-encoding:content-id:
11, 46 -- content-description:resent-date:resent-from:resent-sender:
11, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
11, 46 -- references:list-id:list-help:list-unsubscribe:
11, 46 -- list-subscribe:list-post:list-owner:list-archive;
11, 46 -- z=From:=20 {Colin.Veitch-at-csiro.au} |Subject:=20Manuals=20for
11, 46 -- =20Reichart/Leica=20KF80=20and=20AFS|Date:=20Thu,=207=20M
11, 46 -- ay=202009=2016:27:41=20+1000|Message-ID:=20 {4AA5DD81F68A9
11, 46 -- 34F8C80DA2BF125163D2C37A0C1-at-EXVIC-MBX02.nexus.csiro.au}
11, 46 -- |To:=20 {microscopy-at-microscopy.com} |MIME-Version:=201.0
11, 46 -- |Content-Transfer-Encoding:=20quoted-printable;
11, 46 -- bh=RvDdy8/Vj52J6pGeaZbwUTsC8XOO3ayQg0kgM9NAxq8=;
11, 46 -- b=JgXbJ3y7gxqsyyTnedWb/pZNze8t5keOifwf8EK1Fs6wtDzhWzX8VY5Q
11, 46 -- MK36QAcwXTubNrjAjiCigrPHV2LHQZcgXqm6d/VU5ldgqkE8DIShwAjve
11, 46 -- bdOnGMV6PyFcY8X;
11, 46 -- X-IronPort-AV: E=Sophos;i="4.40,307,1238940000";
11, 46 -- d="scan'208";a="26107652"
11, 46 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
11, 46 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 07 May 2009 16:27:42 +1000
11, 46 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by
11, 46 -- exvic-htca01.nexus.csiro.au ([138.194.81.126]) with mapi; Thu, 7 May 2009
11, 46 -- 16:27:42 +1000
11, 46 -- From: {Colin.Veitch-at-csiro.au}
11, 46 -- To: {microscopy-at-microscopy.com}
11, 46 -- Date: Thu, 7 May 2009 16:27:41 +1000
11, 46 -- Subject: Manuals for Reichart/Leica KF80 and AFS
11, 46 -- Thread-Topic: Manuals for Reichart/Leica KF80 and AFS
11, 46 -- Thread-Index: AcnO3OzI9hMCN6LsTV2/jXN3Ei6DFg==
11, 46 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D2C37A0C1-at-EXVIC-MBX02.nexus.csiro.au}
11, 46 -- Accept-Language: en-US, en-AU
11, 46 -- Content-Language: en-US
11, 46 -- X-MS-Has-Attach:
11, 46 -- X-MS-TNEF-Correlator:
11, 46 -- acceptlanguage: en-US, en-AU
11, 46 -- Content-Type: text/plain; charset="iso-8859-1"
11, 46 -- MIME-Version: 1.0
11, 46 -- Content-Transfer-Encoding: 8bit
11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n476Rhbp027048
==============================End of - Headers==============================




From: gerd-at-biolab.de
Date: Thu, 7 May 2009 10:11:09 -0500
Subject: [Microscopy] SEM EDX window and dewar repair - service resp.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To the knowing,
Putting an Amray ECO-SEM into operation, that was moved and did not run
for several years, the attached EDX-Detector turned out to have a
defective window. It is an Oxford Link Pentafet that was formerly used
for Gun Shot Residue analysis. Has anyone suggestions where to let the
detector repair reasonably (in Europe preferably but not exclusively)?
There are rumours that it is possible to recondition the dewar instead
of replacing it in such a case. Is that recommendable and who will make
that?
Will the SiLi or efferent electronics be affected by the damage?
Any (almost) comment greatly welcomed.

Regards
Gerd
biolab for environmental analysis
Braunschweig, Germany

ps. a bit depressed as after promising start first the BSE, but now the
EDX detector as well turned out to be defective.



==============================Original Headers==============================
5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009
5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47FB79D004161
5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500
5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de [89.183.66.151])
5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis)
5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200
5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de}
5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200
5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de}
5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
5, 19 -- MIME-Version: 1.0
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- Subject: SEM EDX window and dewar repair - service resp.
5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
==============================End of - Headers==============================




From: bfostermme-at-sbcglobal.net
Date: Thu, 7 May 2009 10:48:26 -0500
Subject: [Microscopy] Re: Manuals for Reichart/Leica KF80 and AFS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colin

Try Mager Scientific (in Michigan?)

They have been involved wtih Reichert Histology for decades.

Good hunting
Barbara Foster

Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310 Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now scheduling customized, on-site courses through September. Call me for a free assessment and quote.

At 01:38 AM 5/7/2009, Colin.Veitch-at-csiro.au wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
12, 23 -- From bfostermme-at-sbcglobal.net Thu May 7 10:48:26 2009
12, 23 -- Received: from smtp114.sbc.mail.mud.yahoo.com (smtp114.sbc.mail.mud.yahoo.com [68.142.198.213])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n47FmO6a019969
12, 23 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:48:25 -0500
12, 23 -- Message-Id: {200905071548.n47FmO6a019969-at-ns.microscopy.com}
12, 23 -- Received: (qmail 54225 invoked from network); 7 May 2009 15:48:22 -0000
12, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 23 -- s=s1024; d=sbcglobal.net;
12, 23 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:In-Reply-To:References:Mime-Version:Content-Type;
12, 23 -- b=JADJDhcz62jbzrDdikqina0Si0oDEhA0NiX4Vi1UUnXd2sTsnVHJR2N6m5o7TbH8ZcBv270EVfd+ZNpuzBeHVa2CBwx1frW5/PHdq9sTSQv9Qdh/e1lihub5F84C6zIAeI2ux4kiEb6dyFO/QNXEnqQF4W1mpFSNbVbjqhVFXX0= ;
12, 23 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login)
12, 23 -- by smtp114.sbc.mail.mud.yahoo.com with SMTP; 7 May 2009 15:48:22 -0000
12, 23 -- X-YMail-OSG: gIBYdpYVM1k5yxbGvgaeyCbsnVsy55s2_wr5xXfY2Hxnd6BnmldPwXGTYm.jb24EAcHe4Q4MFZCFsKwWs_EJ.yMccUrIjUgCmBC1JJzJBlpCuD0XzK.5N2KLuWhngbkBnPG6wbtelUM5du5UU1JcX3twqOC4ccPH_Eo3w9SwDRRXtosmb66kAuTYOchshxTKGcmTGuiv8K2u1YqzNZ53azipt8.TBk_lh1e1ksaf.d0aAgNBSYqmVwGPQ5SrMgt9fFIt8FIIZ5cTVCVimCzl_VFrLVjGDVf7amJAb7oZtRoKGFCUqVZAOvaOfPXVHI8saD76rv4iSm4J.3sPDIqWawg-
12, 23 -- X-Yahoo-Newman-Property: ymail-3
12, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 23 -- Date: Thu, 07 May 2009 10:42:24 -0500
12, 23 -- To: Colin.Veitch-at-csiro.au, microscopy-at-microscopy.com
12, 23 -- From: Barbara Foster {bfostermme-at-sbcglobal.net}
12, 23 -- Subject: Re: [Microscopy] Manuals for Reichart/Leica KF80 and AFS
12, 23 -- In-Reply-To: {200905070637.n476bf50006119-at-ns.microscopy.com}
12, 23 -- References: {200905070637.n476bf50006119-at-ns.microscopy.com}
12, 23 -- Mime-Version: 1.0
12, 23 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Thu, 7 May 2009 11:54:06 -0500
Subject: [Microscopy] Flat sections (summary)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to all those who replied to my call for help with sections that
dry down folded in hygroscopic areas of the tissue, such as the hymenium of
many ascomycetes. Most of the replies were off-line, so I will summarize
the suggestions in case they are of interest to others on the list.

Some people thought my tissue was poorly dehydrated or poorly infiltrated.
I have processed thousands of samples of other tissues without problems, so
I'm confident that poor dehydration isn't the issue. When the thick
gelatinous cell wall polysaccharide layer is dehydrated, either by air
drying or in alcohol, the tissue becomes extremely dense. Unlike cells,
where the cytoplasmic water is replaced by ethanol, then resin, the gel of
the hymenium becomes almost solid polysaccharide, and there is little space
left for resin. When the sections are floated on water, the polysaccharides
rehydrate and expand. Some people suggested that I use GMA or another water
soluble resin. I have tried ImmunoBed and JB-4, and the results are the
same. I even tried a crude freeze-substitution (hydrated apothecium frozen
and placed in JB-4 at -20 degrees for a month). Infiltration was poor and I
still had folds.

Several people suggested alternative fluids to float the sections. These
included CCl4, CHCl3, polyethylene glycol, n-butanol, oil, water with high
salt concentration, and water-solvent mixtures. A practical alternative to
water would have to have be safe, dry without residue, float the section and
let it stretch evenly and attach firmly to the slide. So far the solvents I
have tried wet both surfaces of the section and the solvents swell the resin
unevenly, resulting in even more wrinkles.

Techniques to improved stretching of the sections as they dry down was
suggested by several people. These included extending the warming period by
adding water to the droplet on the slide, or by using xylene or chloroform
vapor in addition to heating. One ingenious technique involves inverting
the slide over xylene until the droplet evaporates. Others suggested drying
down the sections slowly. The people who mentioned hotplate temperatures
suggested 45-60 degrees. I have always dried down my sections at just short
of boiling. This works great for "normal" tissue, giving nice flat sections
that adhere well. Perhaps the lower temperature will help with my
ascomycetes.

A published technique for getting flat Epon sections of 4-10 microns
thickness was cited:

Preparation and flattening of thick epoxy sections for light microscopy.
Thompson D. Pizzolato. Can.J.Bot. 54 (20); p2405-2407.1976.

More often people suggested cutting the sections thinner, trimming blocks as
tightly as possible, and notching the periphery of the resin to release
pressure.

I haven't had a chance to try most of these suggestions yet. If any prove
successful, I will let the group know.

Ralph Common
Michigan State University





==============================Original Headers==============================
13, 24 -- From rcommon-at-msu.edu Thu May 7 11:54:05 2009
13, 24 -- Received: from sys53.mail.msu.edu (sys53.mail.msu.edu [35.9.75.233])
13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47Gs5bJ005168
13, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 11:54:05 -0500
13, 24 -- Received: from [35.9.122.125] (helo=emlab)
13, 24 -- by sys53.mail.msu.edu with esmtpsa (Exim 4.63 #12)
13, 24 -- (TLSv1:RC4-MD5:128)
13, 24 -- id 1M26rD-0002bY-QF
13, 24 -- for Microscopy-at-microscopy.com; Thu, 07 May 2009 12:54:03 -0400
13, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
13, 24 -- To: {Microscopy-at-microscopy.com}
13, 24 -- Subject: Flat sections (summary)
13, 24 -- Date: Thu, 7 May 2009 13:53:39 -0400
13, 24 -- Message-ID: {002001c9cf3c$c15bdc20$7d7a0923-at-msu.edu}
13, 24 -- MIME-Version: 1.0
13, 24 -- Content-Type: text/plain;
13, 24 -- charset="iso-8859-1"
13, 24 -- Content-Transfer-Encoding: 7bit
13, 24 -- X-Priority: 3 (Normal)
13, 24 -- X-MSMail-Priority: Normal
13, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
13, 24 -- Importance: Normal
13, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
13, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 7 May 2009 13:42:38 -0500
Subject: [Microscopy] SEM EDX window and dewar repair - service resp.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gerd,
Yes, the dewar can be reconditioned (pumped) and anything else that might
have been damaged when the window went can also be repaired. Although he's
not in Europe, my customers have had good luck with Jim Nicolino in Florida.

Jim Nicolino
AAT
1816 St Johns Bluff Road
Suite 305
Jacksonville, Florida 32246
(904) 646-3069
(904) 646-3131 fax
JNicolino-at-advancedanalysistech.com
http://www.AdvancedAnalysisTech.com

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gerd-at-biolab.de [mailto:gerd-at-biolab.de]
Sent: Thursday, May 07, 2009 11:17 AM
To: kenconverse-at-qualityimages.biz

To the knowing,
Putting an Amray ECO-SEM into operation, that was moved and did not run
for several years, the attached EDX-Detector turned out to have a
defective window. It is an Oxford Link Pentafet that was formerly used
for Gun Shot Residue analysis. Has anyone suggestions where to let the
detector repair reasonably (in Europe preferably but not exclusively)?
There are rumours that it is possible to recondition the dewar instead
of replacing it in such a case. Is that recommendable and who will make
that?
Will the SiLi or efferent electronics be affected by the damage?
Any (almost) comment greatly welcomed.

Regards
Gerd
biolab for environmental analysis
Braunschweig, Germany

ps. a bit depressed as after promising start first the BSE, but now the
EDX detector as well turned out to be defective.



==============================Original Headers==============================
5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009
5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de
[212.227.126.177])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n47FB79D004161
5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08
-0500
5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de
[89.183.66.151])
5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP
(Nemesis)
5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200
5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de}
5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200
5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de}
5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
5, 19 -- MIME-Version: 1.0
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- Subject: SEM EDX window and dewar repair - service resp.
5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
==============================End of - Headers==============================




==============================Original Headers==============================
18, 25 -- From kenconverse-at-qualityimages.biz Thu May 7 13:42:37 2009
18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47IgYmY025359
18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 13:42:36 -0500
18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
18, 25 -- with ESMTP
18, 25 -- id {20090507184232799.NMSF13493-at-cdptpa-omta04.mail.rr.com} ;
18, 25 -- Thu, 7 May 2009 18:42:32 +0000
18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
18, 25 -- To: {gerd-at-biolab.de} , "MSA Listserver" {microscopy-at-microscopy.com}
18, 25 -- Subject: RE: [Microscopy] SEM EDX window and dewar repair - service resp.
18, 25 -- Date: Thu, 7 May 2009 14:42:23 -0400
18, 25 -- Message-ID: {62B60FD54B05484EB82C4868B0D6A56E-at-Ken}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain;
18, 25 -- charset="us-ascii"
18, 25 -- X-Priority: 3 (Normal)
18, 25 -- X-MSMail-Priority: Normal
18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
18, 25 -- In-Reply-To: {200905071517.n47FHC92008323-at-ns.microscopy.com}
18, 25 -- Importance: Normal
18, 25 -- Thread-Index: AcnPJu0Us2YFr5R0Qq61idwxPCzsYQAG+bOw
18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
18, 25 -- Content-Transfer-Encoding: 8bit
18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n47IgYmY025359
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 7 May 2009 14:40:07 -0500
Subject: [Microscopy] Re: SEM EDX window and dewar repair - service resp.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gerd,
feel free to get in contact with
Marco Mostert - SAMx
at Bruchsal:

Silberhoelle 7
D-76646 Bruchsal, Germany
Email: mm-at-samx.com

He is an EDS specialist doing repair on windows, pumping dewars etc.
Just a satisfied customer.

Yours, Stefan Diller





gerd-at-biolab.de schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} To the knowing,
} Putting an Amray ECO-SEM into operation, that was moved and did not run
} for several years, the attached EDX-Detector turned out to have a
} defective window. It is an Oxford Link Pentafet that was formerly used
} for Gun Shot Residue analysis. Has anyone suggestions where to let the
} detector repair reasonably (in Europe preferably but not exclusively)?
} There are rumours that it is possible to recondition the dewar instead
} of replacing it in such a case. Is that recommendable and who will make
} that?
} Will the SiLi or efferent electronics be affected by the damage?
} Any (almost) comment greatly welcomed.
}
} Regards
} Gerd
} biolab for environmental analysis
} Braunschweig, Germany
}
} ps. a bit depressed as after promising start first the BSE, but now the
} EDX detector as well turned out to be defective.
}
}
}
} ==============================Original Headers==============================
} 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009
} 5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177])
} 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47FB79D004161
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500
} 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de [89.183.66.151])
} 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis)
} 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200
} 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de}
} 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200
} 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de}
} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
} 5, 19 -- MIME-Version: 1.0
} 5, 19 -- To: Microscopy-at-microscopy.com
} 5, 19 -- Subject: SEM EDX window and dewar repair - service resp.
} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
} 5, 19 -- Content-Transfer-Encoding: 7bit
} 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
} 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
} 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
} ==============================End of - Headers==============================
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
11, 22 -- From stefan.diller-at-t-online.de Thu May 7 14:40:05 2009
11, 22 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47Je3df009672
11, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 14:40:04 -0500
11, 22 -- Received: from fwd05.aul.t-online.de
11, 22 -- by mailout07.sul.t-online.de with smtp
11, 22 -- id 1M29Rq-0008N0-00; Thu, 07 May 2009 21:40:02 +0200
11, 22 -- Received: from stefan-dillers-mac-pro.local (SarmrBZGQh12ao+GrhIxFTsId7ON5L3ydrAVkPOZEdw-CAeJ6PMHil01hHCxEe-ZTc-at-[91.10.197.175]) by fwd05.aul.t-online.de
11, 22 -- with esmtp id 1M29Ra-1SVZ3I0; Thu, 7 May 2009 21:39:46 +0200
11, 22 -- Message-ID: {4A033902.7050608-at-t-online.de}
11, 22 -- Date: Thu, 07 May 2009 21:39:46 +0200
11, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
11, 22 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
11, 22 -- MIME-Version: 1.0
11, 22 -- To: microscopy-at-microscopy.com
11, 22 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair - service resp.
11, 22 -- References: {200905071522.n47FMBkW011622-at-ns.microscopy.com}
11, 22 -- In-Reply-To: {200905071522.n47FMBkW011622-at-ns.microscopy.com}
11, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
11, 22 -- Content-Transfer-Encoding: 7bit
11, 22 -- X-ID: SarmrBZGQh12ao+GrhIxFTsId7ON5L3ydrAVkPOZEdw-CAeJ6PMHil01hHCxEe-ZTc
11, 22 -- X-TOI-MSGID: ab230404-201d-47c7-807f-97d0636517ab
==============================End of - Headers==============================




From: dkloos-at-parallaxray.com
Date: Thu, 7 May 2009 15:52:41 -0500
Subject: [Microscopy] PIXE Referral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My message today is motivated by questions asked by a quality systems
auditor who recently visited my lab.



We calibrate the XYZ scan axes of our AFM using a traceable height and pitch
standard made of thermally grown silicon dioxide on silicon. The specimen
contains these patterns:

10 um pitch, 2-dimensional array of pits, nominally 200 nm deep

2 um pitch, 1-dimensional array of ridges, same depth as the pits.



The manufacturer's certificate of traceability does not discuss
recalibration or indicate any need for it.

Based on the materials of construction, I believe that the pitch values and
the pit depth are stable and that no recalibration is needed, provided that
we are careful in handling and using the specimen. For example, any
nanodebris visible in the image is excluded from the measurement.

However, the quality consultant says that it is common to send such samples
to an outside lab for recalibration. He added that we should either do
recalibration or write a justification for why recalibration is not
necessary.



I am curious what other people do about this. One can consider this in
several different environments:

-the AFM or SEM manufacturer, who wants to provide good calibration for the
instruments

-the AFM or SEM user who is supporting a production or QC application

-the AFM or SEM user who is mainly supporting R&D, but needs to show
traceability in order to meet the requirements of a quality management
system.



Here are a few practical questions to start the discussion.

If you have such a standard recalibrated, what is the time interval?

If you do not recalibrate the standard, what rationale do you give for why
it is unnecessary?

Can you articulate the reasons so that it is clear they are based on good
science and engineering data and practices?

Do you have any other comments on this subject?



regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
28, 24 -- From donc-at-asmicro.com Thu May 7 15:00:09 2009
28, 24 -- Received: from smtp107.sbc.mail.re2.yahoo.com (smtp107.sbc.mail.re2.yahoo.com [68.142.229.98])
28, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n47K083K024768
28, 24 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 15:00:08 -0500
28, 24 -- Received: (qmail 26126 invoked from network); 7 May 2009 20:00:07 -0000
28, 24 -- Received: from unknown (HELO asm15) (donc-at-76.252.13.152 with login)
28, 24 -- by smtp107.sbc.mail.re2.yahoo.com with SMTP; 7 May 2009 20:00:06 -0000
28, 24 -- X-YMail-OSG: fZcAt_sVM1lsgrq9MF2qfzW.vMZG5.iYsStX5U1x93tUxd.JHrJEs_7vA1ZtmxMQA4MWFzhH2VQv21ZPWg3.kmzepUFSSrjvirWqiMAwJZePR4xZ4T0RWCnXBXM7SfUUplmGQNA4kAxcNPp3WAWPbw9YskCenLVT2vPSqLu8F2NHijlgw5GZEewdSDniOWtX6cyb_r34qDwlY.rHV.SePToqH_zna6c4WyvCZuFDZzlpg9XNJqYwajxNpiPuoZou.I4gbJYoXaIer.FGJfarStBxAS9GyExqkjlJeVbEn_U5cECnzzNCp9isdVZBjn4WU1Z_yWcihTRE6Y_BzzXQAiI5wu6TKN7nwnC_
28, 24 -- X-Yahoo-Newman-Property: ymail-3
28, 24 -- Message-ID: {FDB45F447CC049829760E9C1386EF7F4-at-asm15}
28, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
28, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
28, 24 -- Subject: Recalibration of AFM and SEM Calibration Standards
28, 24 -- Date: Thu, 7 May 2009 15:59:13 -0400
28, 24 -- MIME-Version: 1.0
28, 24 -- Content-Type: text/plain;
28, 24 -- format=flowed;
28, 24 -- charset="iso-8859-1";
28, 24 -- reply-type=original
28, 24 -- Content-Transfer-Encoding: 7bit
28, 24 -- X-Priority: 3
28, 24 -- X-MSMail-Priority: Normal
28, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
28, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================

From cxzeriqqrbpxts5qof8kr-at-washington.edu Thu May 7 15:04:41 2009
Return-Path: {cxzeriqqrbpxts5qof8kr-at-washington.edu}
Received: from google.com (122-121-240-221.dynamic.hinet.net [122.121.240.221])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47K4efb031284
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 7 May 2009 15:04:41 -0500
Received: from [190.102.27.161] (HELO google.com)
by cold-girlie.de; Fri, 8 May 2009 04:04:25 +0800

I have an associate who needs PIXE equipment. Do any of you have any
suggestions for vendors of such an instrument? Also, how about an agent or
rep for such instrument in Hong Kong area?

Thanks

Don Kloos
VP Sales, Marketing, Business Development
Parallax Research, Inc.
 
 
Sales & Marketing
16478 Beach Blvd. #330
Westminster, California, 92683-7860 USA
 
TOLL FREE 1 866 581-XRAY (9729)
Telephone 1 714 897-9779
Fax 1 714 897-1421
Email: dkloos-at-parallaxray.com
SKYPE: don.kloos
Website: http://www.parallaxray.com
 
 




==============================Original Headers==============================
6, 29 -- From dkloos-at-parallaxray.com Thu May 7 15:52:41 2009
6, 29 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54])
6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47KqfUK008838
6, 29 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 15:52:41 -0500
6, 29 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl)
6, 29 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD))
6, 29 -- (envelope-from {dkloos-at-parallaxray.com} )
6, 29 -- id 1M2AaE-0008VE-CD
6, 29 -- for microscopy-at-microscopy.com; Thu, 07 May 2009 14:52:46 -0600
6, 29 -- Reply-To: {dkloos-at-parallaxray.com}
6, 29 -- From: "Don Kloos" {dkloos-at-parallaxray.com}
6, 29 -- To: {microscopy-at-microscopy.com}
6, 29 -- Subject: PIXE Referral
6, 29 -- Date: Thu, 7 May 2009 13:52:35 -0700
6, 29 -- Organization: Parallax Research
6, 29 -- Message-ID: {6B1B5A3341054EFB9DD0128C20386534-at-donl}
6, 29 -- MIME-Version: 1.0
6, 29 -- Content-Type: text/plain;
6, 29 -- charset="iso-8859-1"
6, 29 -- X-Mailer: Microsoft Office Outlook 11
6, 29 -- Thread-Index: AcnPVb+xLcFDh9AQRwSf+0pML11ccg==
6, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
6, 29 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
6, 29 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com
6, 29 -- X-AntiAbuse: Original Domain - microscopy.com
6, 29 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6]
6, 29 -- X-AntiAbuse: Sender Address Domain - parallaxray.com
6, 29 -- Content-Transfer-Encoding: 8bit
6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n47KqfUK008838
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Thu, 7 May 2009 23:30:38 -0500
Subject: [Microscopy] KF80/AFS thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Folks,

Thank you so much for the responses to my request for the manuals for the above instruments. I now have the manuals for both from a variety of sources!

All I have to do now is try and find all the bits and put them together!

Cheers from a slightly damp Geelong - rain is still a bit of a novelty here!!



Colin Veitch
Electron Microscopist
CSIRO Materials Science and Engineering, Geelong Laboratory
PO Box 21, BELMONT, Vic. 3216. Australia.
colin.veitch-at-csiro.au

Tel: +61 (0) 3 5246 4000  
Mobile: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.



==============================Original Headers==============================
11, 46 -- From prvs=37297e90c=Colin.Veitch-at-csiro.au Thu May 7 23:30:37 2009
11, 46 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37])
11, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n484UaOt009679
11, 46 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 23:30:37 -0500
11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
11, 46 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt;
11, 46 -- s=email; t=1241757037; x=1273293037;
11, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
11, 46 -- mime-version:content-transfer-encoding:content-id:
11, 46 -- content-description:resent-date:resent-from:resent-sender:
11, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
11, 46 -- references:list-id:list-help:list-unsubscribe:
11, 46 -- list-subscribe:list-post:list-owner:list-archive;
11, 46 -- z=From:=20 {Colin.Veitch-at-csiro.au} |Subject:=20KF80/AFS=20th
11, 46 -- ank=20you|Date:=20Fri,=208=20May=202009=2014:30:34=20+100
11, 46 -- 0|Message-ID:=20 {4AA5DD81F68A934F8C80DA2BF125163D2C37A0C4
11, 46 -- -at-EXVIC-MBX02.nexus.csiro.au} |To:=20 {microscopy-at-microscopy
11, 46 -- .com} |MIME-Version:=201.0|Content-Transfer-Encoding:=20qu
11, 46 -- oted-printable;
11, 46 -- bh=XnmgzdwhD3r0JiZGGiiEgpNESGak6N8kDe0b6Dbd6HU=;
11, 46 -- b=KqC/wBcVGO/Si3ZvTaSfLa2wNmF8qP88NUR6yWZe+zfXOVYTm28JwZD0
11, 46 -- 0y0O+Z4R6FvAPW8717uH0cESZNCoqhVzAC+Z/3Pwq2fdTh6PqjmxpV9mm
11, 46 -- HMMFZF7mdgqQN/M;
11, 46 -- X-IronPort-AV: E=Sophos;i="4.40,315,1238940000";
11, 46 -- d="scan'208";a="13873139"
11, 46 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
11, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 08 May 2009 14:30:35 +1000
11, 46 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by
11, 46 -- exvic-htca01.nexus.csiro.au ([138.194.81.126]) with mapi; Fri, 8 May 2009
11, 46 -- 14:30:35 +1000
11, 46 -- From: {Colin.Veitch-at-csiro.au}
11, 46 -- To: {microscopy-at-microscopy.com}
11, 46 -- Date: Fri, 8 May 2009 14:30:34 +1000
11, 46 -- Subject: KF80/AFS thank you
11, 46 -- Thread-Topic: KF80/AFS thank you
11, 46 -- Thread-Index: AcnPlbj1FdD6HATKSDqWnmae/uL33g==
11, 46 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D2C37A0C4-at-EXVIC-MBX02.nexus.csiro.au}
11, 46 -- Accept-Language: en-US, en-AU
11, 46 -- Content-Language: en-US
11, 46 -- X-MS-Has-Attach:
11, 46 -- X-MS-TNEF-Correlator:
11, 46 -- acceptlanguage: en-US, en-AU
11, 46 -- Content-Type: text/plain; charset="iso-8859-1"
11, 46 -- MIME-Version: 1.0
11, 46 -- Content-Transfer-Encoding: 8bit
11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n484UaOt009679
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Fri, 8 May 2009 02:55:14 -0500
Subject: [Microscopy] Re: SEM EDX window and dewar repair - service resp.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gerd


I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am
very satisfied with it. They manufacture detectors, and will also
repair and upgrade old ones.

Gresham, in the UK, used to do these also but I think perhaps they
don't after having been taken over by ev2.

Oxford would be the obvious people to ask first, as I think their
Pentafet detectors may be tricky to interface to other preamps
(something about needing an externally generated reset pulse?), but
are they still in the EDS business since they were taken over by Gattan?

If you're buying a new one, check out the experiences of others, as
some brands are reputed to have dewars more likely to lose their
vacuum than others.


good luck

Ritchie Sims
Geology
University of Auckland
Auckland, New Zealand

Quoting gerd-at-biolab.de:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} To the knowing,
} Putting an Amray ECO-SEM into operation, that was moved and did not run
} for several years, the attached EDX-Detector turned out to have a
} defective window. It is an Oxford Link Pentafet that was formerly used
} for Gun Shot Residue analysis. Has anyone suggestions where to let the
} detector repair reasonably (in Europe preferably but not exclusively)?
} There are rumours that it is possible to recondition the dewar instead
} of replacing it in such a case. Is that recommendable and who will make
} that?
} Will the SiLi or efferent electronics be affected by the damage?
} Any (almost) comment greatly welcomed.
}
} Regards
} Gerd
} biolab for environmental analysis
} Braunschweig, Germany
}
} ps. a bit depressed as after promising start first the BSE, but now the
} EDX detector as well turned out to be defective.
}
}
}
} ==============================Original Headers==============================
} 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009
} 5, 19 -- Received: from moutng.kundenserver.de
} (moutng.kundenserver.de [212.227.126.177])
} 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n47FB79D004161
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500
} 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de
} [89.183.66.151])
} 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis)
} 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200
} 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de}
} 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200
} 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de}
} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
} 5, 19 -- MIME-Version: 1.0
} 5, 19 -- To: Microscopy-at-microscopy.com
} 5, 19 -- Subject: SEM EDX window and dewar repair - service resp.
} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
} 5, 19 -- Content-Transfer-Encoding: 7bit
} 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
} 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
} 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
} ==============================End of - Headers==============================
}



----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.


==============================Original Headers==============================
15, 41 -- From r.sims-at-auckland.ac.nz Fri May 8 02:55:14 2009
15, 41 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n487tD71004433
15, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 02:55:14 -0500
15, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1])
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 568B466916C;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
15, 41 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
15, 41 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
15, 41 -- with ESMTP id 9CQIf4N4HMCZ; Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (uxchorprd01.its.auckland.ac.nz [130.216.10.20])
15, 41 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
15, 41 -- (No client certificate requested)
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 26BE166916B;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (localhost.localdomain [127.0.0.1])
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8) with ESMTP id n487t71L025540;
15, 41 -- Fri, 8 May 2009 19:55:07 +1200
15, 41 -- Received: (from apache-at-localhost)
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8/Submit) id n487t5GT025539;
15, 41 -- Fri, 8 May 2009 19:55:05 +1200
15, 41 -- X-Authentication-Warning: uxchorprd01.its.auckland.ac.nz: apache set sender to r.sims-at-auckland.ac.nz using -f
15, 41 -- Received: from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz
15, 41 -- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with HTTP;
15, 41 -- Fri, 08 May 2009 19:55:05 +1200
15, 41 -- Message-ID: {20090508195505.46401850sghpdh7k-at-webmail.auckland.ac.nz}
15, 41 -- Date: Fri, 08 May 2009 19:55:05 +1200
15, 41 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz}
15, 41 -- To: Microscopy-at-microscopy.com
15, 41 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair - service resp.
15, 41 -- References: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- In-Reply-To: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- MIME-Version: 1.0
15, 41 -- Content-Type: text/plain;
15, 41 -- charset=ISO-8859-1;
15, 41 -- DelSp="Yes";
15, 41 -- format="flowed"
15, 41 -- Content-Disposition: inline
15, 41 -- Content-Transfer-Encoding: 7bit
15, 41 -- User-Agent: Internet Messaging Program (IMP) 4.3.3
==============================End of - Headers==============================




From: Peter.Smith-at-e2v.com
Date: Fri, 8 May 2009 03:32:20 -0500
Subject: [Microscopy] Re: SEM EDX window and dewar repair - service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ritchie

Just to set the record straight, Gresham Scientific Instruments Ltd was
acquired in July 2005 by e2v technologies plc and subsequently changed
its name to e2v scientific instruments Ltd in March 2006.

E2v scientific instruments Ltd has not changed its business profile in
any way and continues to offer a high quality worldwide EDX detector
repair service in addition to its manufactured products.

Kind regards
Peter

______________
Dr Peter Smith
Sales Director

e2v scientific instruments ltd
Sirius House, Watery Lane
Wooburn Green, High Wycombe, Bucks, HP10 0AP, UK

Tel: +44 (0)1628 533060 x219
Fax: +44(0)1628 533034
Mobile: +44 (0)7771 762343
Email: peter.smith-at-e2v.com
Web site: www.e2vsi.com

This communication is for the exclusive use of the intended recipient(s)
and contains information that is confidential and may also be
privileged. If you are not the intended recipient(s) please note that
any form of distribution, copying or use of this communication, the
information in it or in attachments is strictly prohibited and may be
unlawful. If you have received this communication in error please
return it to the sender, and then delete all copies of the email and its
attachments.
Registered office: 106 Waterhouse Lane, Chelmsford, Essex, CM1 2QU
England. Company reg. no. 344963. VAT no. GB669490581.


-----Original Message-----
X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz]
Sent: 08 May 2009 09:07
To: Smith, Peter

Hi Gerd


I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am
very satisfied with it. They manufacture detectors, and will also repair
and upgrade old ones.

Gresham, in the UK, used to do these also but I think perhaps they don't
after having been taken over by ev2.

Oxford would be the obvious people to ask first, as I think their
Pentafet detectors may be tricky to interface to other preamps
(something about needing an externally generated reset pulse?), but are
they still in the EDS business since they were taken over by Gattan?

If you're buying a new one, check out the experiences of others, as some
brands are reputed to have dewars more likely to lose their vacuum than
others.


good luck

Ritchie Sims
Geology
University of Auckland
Auckland, New Zealand

Quoting gerd-at-biolab.de:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society

} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} To the knowing,
} Putting an Amray ECO-SEM into operation, that was moved and did not
} run for several years, the attached EDX-Detector turned out to have a
} defective window. It is an Oxford Link Pentafet that was formerly used

} for Gun Shot Residue analysis. Has anyone suggestions where to let the

} detector repair reasonably (in Europe preferably but not exclusively)?
} There are rumours that it is possible to recondition the dewar instead

} of replacing it in such a case. Is that recommendable and who will
} make that?
} Will the SiLi or efferent electronics be affected by the damage?
} Any (almost) comment greatly welcomed.
}
} Regards
} Gerd
} biolab for environmental analysis
} Braunschweig, Germany
}
} ps. a bit depressed as after promising start first the BSE, but now
} the EDX detector as well turned out to be defective.
}
}
}
} ==============================Original
} Headers==============================
} 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 --
} Received: from moutng.kundenserver.de (moutng.kundenserver.de
} [212.227.126.177])
} 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP
} id n47FB79D004161
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009
10:11:08 -0500
} 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de
} [89.183.66.151])
} 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP
(Nemesis)
} 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06
+0200
} 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu,
} 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen
} {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21
} (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To:
} Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar
} repair - service resp.
} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
} 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID:
} V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
} 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
} 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
} ==============================End of -
} Headers==============================
}



----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.


==============================Original
Headers==============================
15, 41 -- From r.sims-at-auckland.ac.nz Fri May 8 02:55:14 2009 15, 41 --
Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz
[130.216.12.33])
15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n487tD71004433
15, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009
02:55:14 -0500
15, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1])
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
568B466916C;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 15,
41 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
15, 41 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1])
(amavisd-new, port 10024)
15, 41 -- with ESMTP id 9CQIf4N4HMCZ; Fri, 8 May 2009 19:55:08
+1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz
(uxchorprd01.its.auckland.ac.nz [130.216.10.20])
15, 41 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256
bits))
15, 41 -- (No client certificate requested)
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
26BE166916B;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz
(localhost.localdomain [127.0.0.1])
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8) with
ESMTP id n487t71L025540;
15, 41 -- Fri, 8 May 2009 19:55:07 +1200
15, 41 -- Received: (from apache-at-localhost)
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8/Submit)
id n487t5GT025539;
15, 41 -- Fri, 8 May 2009 19:55:05 +1200
15, 41 -- X-Authentication-Warning: uxchorprd01.its.auckland.ac.nz:
apache set sender to r.sims-at-auckland.ac.nz using -f 15, 41 -- Received:
from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz 15, 41
-- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with
HTTP; 15, 41 -- Fri, 08 May 2009 19:55:05 +1200 15, 41 -- Message-ID:
{20090508195505.46401850sghpdh7k-at-webmail.auckland.ac.nz}
15, 41 -- Date: Fri, 08 May 2009 19:55:05 +1200 15, 41 -- From: Ritchie
Sims {r.sims-at-auckland.ac.nz} 15, 41 -- To: Microscopy-at-microscopy.com 15,
41 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair -
service resp.
15, 41 -- References: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- In-Reply-To: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- MIME-Version: 1.0
15, 41 -- Content-Type: text/plain;
15, 41 -- charset=ISO-8859-1;
15, 41 -- DelSp="Yes";
15, 41 -- format="flowed"
15, 41 -- Content-Disposition: inline
15, 41 -- Content-Transfer-Encoding: 7bit 15, 41 -- User-Agent: Internet
Messaging Program (IMP) 4.3.3 ==============================End of -
Headers==============================

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________

Sent by a member of the e2v group of companies. The parent company, e2v technologies plc, is registered in England and Wales. Company number; 04439718. Registered address; 106 Waterhouse Lane, Chelmsford, Essex, CM1 2QU, UK. This email and any attachments are confidential and meant solely for the use of the intended recipient. If you are not the intended recipient and have received this email in error, please notify us immediately by replying to the sender and then deleting this copy and the reply from your system without further disclosing, copying, distributing or using the e-mail or any attachment. Thank you for your cooperation.
______________________________________________________
________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
36, 33 -- From Peter.Smith-at-e2v.com Fri May 8 03:32:20 2009
36, 33 -- Received: from mail195.messagelabs.com (mail195.messagelabs.com [85.158.138.147])
36, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n488WIBH019943
36, 33 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 03:32:19 -0500
36, 33 -- X-VirusChecked: Checked
36, 33 -- X-Env-Sender: Peter.Smith-at-e2v.com
36, 33 -- X-Msg-Ref: server-13.tower-195.messagelabs.com!1241771538!13955364!1
36, 33 -- X-StarScan-Version: 6.0.0; banners=e2v.com,-,-
36, 33 -- X-Originating-IP: [213.48.15.234]
36, 33 -- Received: (qmail 3315 invoked from network); 8 May 2009 08:32:18 -0000
36, 33 -- Received: from host.e2v.com (HELO WHL32.e2v.com) (213.48.15.234)
36, 33 -- by server-13.tower-195.messagelabs.com with SMTP; 8 May 2009 08:32:18 -0000
36, 33 -- Received: from WHL46.e2v.com ([162.1.51.83]) by WHL32.e2v.com with Microsoft SMTPSVC(6.0.3790.1830);
36, 33 -- Fri, 8 May 2009 09:32:18 +0100
36, 33 -- Received: from WYCOM3.e2v.com ([162.1.11.6]) by WHL46.e2v.com with Microsoft SMTPSVC(6.0.3790.1830);
36, 33 -- Fri, 8 May 2009 09:32:17 +0100
36, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
36, 33 -- Content-class: urn:content-classes:message
36, 33 -- MIME-Version: 1.0
36, 33 -- Content-Type: text/plain;
36, 33 -- charset="us-ascii"
36, 33 -- Subject: [Microscopy] Re: SEM EDX window and dewar repair - service resp.
36, 33 -- Date: Fri, 8 May 2009 09:32:16 +0100
36, 33 -- Message-ID: {112184A2B7A9204CBE5F3B4DB06C4DFF6646E4-at-WYCOM3.e2v.com}
36, 33 -- X-MS-Has-Attach:
36, 33 -- X-MS-TNEF-Correlator:
36, 33 -- Thread-Topic: [Microscopy] Re: SEM EDX window and dewar repair - service resp.
36, 33 -- thread-index: AcnPs+s4rGdPmxUQRaKJjMuIvkaNhAAAijQg
36, 33 -- From: "Smith, Peter" {Peter.Smith-at-e2v.com}
36, 33 -- To: {microscopy-at-microscopy.com}
36, 33 -- X-OriginalArrivalTime: 08 May 2009 08:32:17.0707 (UTC) FILETIME=[7F375FB0:01C9CFB7]
36, 33 -- Content-Transfer-Encoding: 8bit
36, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n488WIBH019943
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Fri, 8 May 2009 04:39:09 -0500
Subject: [Microscopy] Re: SEM EDX window and dewar repair - service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oxford Instruments still handle EDX. Their cryo business was taken
over by Gatan.

Dave

-----Original Message-----
X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz]
Sent: 08 May 2009 09:00
To: David Patton

Hi Gerd


I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am
very satisfied with it. They manufacture detectors, and will also repair
and upgrade old ones.

Gresham, in the UK, used to do these also but I think perhaps they don't
after having been taken over by ev2.

Oxford would be the obvious people to ask first, as I think their
Pentafet detectors may be tricky to interface to other preamps
(something about needing an externally generated reset pulse?), but are
they still in the EDS business since they were taken over by Gattan?

If you're buying a new one, check out the experiences of others, as some
brands are reputed to have dewars more likely to lose their vacuum than
others.


good luck

Ritchie Sims
Geology
University of Auckland
Auckland, New Zealand

Quoting gerd-at-biolab.de:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society

} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} To the knowing,
} Putting an Amray ECO-SEM into operation, that was moved and did not
} run for several years, the attached EDX-Detector turned out to have a
} defective window. It is an Oxford Link Pentafet that was formerly used

} for Gun Shot Residue analysis. Has anyone suggestions where to let the

} detector repair reasonably (in Europe preferably but not exclusively)?
} There are rumours that it is possible to recondition the dewar instead

} of replacing it in such a case. Is that recommendable and who will
} make that?
} Will the SiLi or efferent electronics be affected by the damage?
} Any (almost) comment greatly welcomed.
}
} Regards
} Gerd
} biolab for environmental analysis
} Braunschweig, Germany
}
} ps. a bit depressed as after promising start first the BSE, but now
} the EDX detector as well turned out to be defective.
}
}
}
} ==============================Original
} Headers==============================
} 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 --
} Received: from moutng.kundenserver.de (moutng.kundenserver.de
} [212.227.126.177])
} 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP
} id n47FB79D004161
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009
10:11:08 -0500
} 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de
} [89.183.66.151])
} 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP
(Nemesis)
} 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06
+0200
} 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu,
} 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen
} {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21
} (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To:
} Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar
} repair - service resp.
} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
} 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID:
} V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59
} 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO
} 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx
} ==============================End of -
} Headers==============================
}



----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.


==============================Original
Headers==============================
15, 41 -- From r.sims-at-auckland.ac.nz Fri May 8 02:55:14 2009 15, 41 --
Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz
[130.216.12.33])
15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n487tD71004433
15, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009
02:55:14 -0500
15, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1])
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
568B466916C;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 15,
41 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
15, 41 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1])
(amavisd-new, port 10024)
15, 41 -- with ESMTP id 9CQIf4N4HMCZ; Fri, 8 May 2009 19:55:08
+1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz
(uxchorprd01.its.auckland.ac.nz [130.216.10.20])
15, 41 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256
bits))
15, 41 -- (No client certificate requested)
15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id
26BE166916B;
15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST)
15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz
(localhost.localdomain [127.0.0.1])
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8) with
ESMTP id n487t71L025540;
15, 41 -- Fri, 8 May 2009 19:55:07 +1200
15, 41 -- Received: (from apache-at-localhost)
15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8/Submit)
id n487t5GT025539;
15, 41 -- Fri, 8 May 2009 19:55:05 +1200
15, 41 -- X-Authentication-Warning: uxchorprd01.its.auckland.ac.nz:
apache set sender to r.sims-at-auckland.ac.nz using -f 15, 41 -- Received:
from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz 15, 41
-- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with
HTTP; 15, 41 -- Fri, 08 May 2009 19:55:05 +1200 15, 41 -- Message-ID:
{20090508195505.46401850sghpdh7k-at-webmail.auckland.ac.nz}
15, 41 -- Date: Fri, 08 May 2009 19:55:05 +1200 15, 41 -- From: Ritchie
Sims {r.sims-at-auckland.ac.nz} 15, 41 -- To: Microscopy-at-microscopy.com 15,
41 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair -
service resp.
15, 41 -- References: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- In-Reply-To: {200905071513.n47FDGK5005559-at-ns.microscopy.com}
15, 41 -- MIME-Version: 1.0
15, 41 -- Content-Type: text/plain;
15, 41 -- charset=ISO-8859-1;
15, 41 -- DelSp="Yes";
15, 41 -- format="flowed"
15, 41 -- Content-Disposition: inline
15, 41 -- Content-Transfer-Encoding: 7bit 15, 41 -- User-Agent: Internet
Messaging Program (IMP) 4.3.3 ==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
31, 36 -- From David.Patton-at-uwe.ac.uk Fri May 8 04:39:08 2009
31, 36 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n489d6PM004673
31, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 04:39:07 -0500
31, 36 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
31, 36 -- id 412d_1013469e_3bb4_11de_8c18_00142223915c;
31, 36 -- Fri, 08 May 2009 10:39:04 +0100
31, 36 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
31, 36 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
31, 36 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
31, 36 -- 2005)) with SMTP id {0KJB002TIK4UJQ-at-mta01.uwe.ac.uk} for
31, 36 -- Microscopy-at-microscopy.com; Fri, 08 May 2009 10:38:56 +0100 (BST)
31, 36 -- Date: Fri, 08 May 2009 10:36:34 +0100
31, 36 -- From: David Patton {David.Patton-at-uwe.ac.uk}
31, 36 -- Subject: RE: [Microscopy] Re: SEM EDX window and dewar repair - service resp.
31, 36 -- re Oxford Instruments
31, 36 -- In-reply-to: {200905080800.n488014r010186-at-ns.microscopy.com}
31, 36 -- To: r.sims-at-auckland.ac.nz
31, 36 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF85203-at-egen-uwe01}
31, 36 -- MIME-version: 1.0
31, 36 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
31, 36 -- Content-type: text/plain; charset=US-ASCII
31, 36 -- Content-class: urn:content-classes:message
31, 36 -- Thread-topic: [Microscopy] Re: SEM EDX window and dewar repair - service resp.
31, 36 -- re Oxford Instruments
31, 36 -- Thread-index: AcnPswRJklZ0/o97T+SEq7iNrbUPBgADTcKw
31, 36 -- X-MS-Has-Attach:
31, 36 -- X-MS-TNEF-Correlator:
31, 36 -- References: {200905080800.n488014r010186-at-ns.microscopy.com}
31, 36 -- X-NAIMIME-Disclaimer: 1
31, 36 -- X-NAIMIME-Modified: 1
31, 36 -- X-NAI-Spam-Score: 0
31, 36 -- X-NAI-Spam-Rules: 1 Rules triggered
31, 36 -- RV3270=0
31, 36 -- Content-Transfer-Encoding: 8bit
31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n489d6PM004673
==============================End of - Headers==============================




From: eawoodruff-at-live.com
Date: Fri, 8 May 2009 07:41:46 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both eawoodruff-at-live.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: eawoodruff-at-live.com
Name: Elvin Woodruff III

Organization: Tennessee State University

Title-Subject: [Filtered] Nitrogen leak

Question: Hi All,

I have recently started working with a JEOL 6701F scanning electron
microscopy. The scope is running just fine, but it is going through
nitrogen gas like there is not tomorrow. The scope sits on an air
table which is supplied by gas (could this be it?)and other areas
such as the specimen load chamber is also filled with nitrogen gas. I
have talked to a couple of other people about the gas loss but to no
avail. I have checked all of the usual places for a leak but can't
find anything. We don't have a service contract right now so any help
or ideas on why we are going through so much gas would be a big help.

Thanks

Elvin

Login Host: 68.19.196.245
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:41:46 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48CfjvE003327
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 07:41:46 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c629d8f35379-at-[206.69.208.22]}
9, 11 -- Date: Fri, 8 May 2009 07:41:44 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: eawoodruff-at-live.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Nitrogen leak
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: reznik-at-ict.uni-karlsruhe.de
Date: Fri, 8 May 2009 07:42:15 -0500
Subject: [Microscopy] viaWWW: aperture_cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both reznik-at-ict.uni-karlsruhe.de as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: reznik-at-ict.uni-karlsruhe.de
Name: Reznik Boris

Organization: University Karlsruhe

Title-Subject: [Filtered] aperture_cleaning

Question: Hi Folks,

Thank you very much for your advices !

Login Host: 129.13.186.3
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:42:15 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48CgEsN003941
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 07:42:15 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c629d9115a66-at-[206.69.208.22]}
7, 11 -- Date: Fri, 8 May 2009 07:42:14 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: reznik-at-ict.uni-karlsruhe.de (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: aperture_cleaning
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Fri, 8 May 2009 10:29:38 -0500
Subject: [Microscopy] Recalibration of AFM and SEM Calibration Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Elvin,

Have you checked the tube of the air table where nitrogen is vented when you
bump into the scope or put something on the scope console?
I had an air table for my ultra-microtome and at one time found that there
was a small, noiseless stream of gas coming out of the small tube when there
was no disturbance. The lever beside that tube which touches the bottom of
the table was not going totally back to the closed/up position and needed a
small bit of lubrication.

You mentioned that you have checked several places for the leak.
Do you have tubing connecting the tank to your scope that is of the proper
material to contain nitrogen? I do not recall the name/type of the tubing
that I used but it was milky white in color, not very flexible and about 8mm
in diameter. Someone on the List will most likely tell us the name.

Let us know when you do find out what is causing your gas to be used up so
quickly.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do
not represent the NIH. This message is not
confidential and can be freely shared and reproduced.
------------------------------------------------------------------------
X-from: {eawoodruff-at-live.com}
Reply-To: {eawoodruff-at-live.com}

Who (re)calibrates these quality auditors? What certification do they
have? It seems like this auditor is out of their element.

Maybe I am a little flip to think that the burden of proof should go the
other way. We could assume that a standard would still be valid unless
it has been demonstrated that it can go out of calibration over some
period of time. Frankly, I cannot understand why someone would think a
piece of silicon would change dimension on that scale because it sat on
a shelf for 10 years. I think diffusion is a little slower than that.

Disclaimer: Our lab has come under such rigorous, externally supervised
quality control. We use our experience to check the quality of our own
work. I daresay we do as well, and maybe better, than some labs that
pass the auditor's review.

Warren S.

-----Original Message-----
X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com]
Sent: Thursday, May 07, 2009 3:01 PM
To: wesaia-at-iastate.edu

My message today is motivated by questions asked by a quality systems
auditor who recently visited my lab.



We calibrate the XYZ scan axes of our AFM using a traceable height and
pitch
standard made of thermally grown silicon dioxide on silicon. The
specimen
contains these patterns:

10 um pitch, 2-dimensional array of pits, nominally 200 nm deep

2 um pitch, 1-dimensional array of ridges, same depth as the pits.



The manufacturer's certificate of traceability does not discuss
recalibration or indicate any need for it.

Based on the materials of construction, I believe that the pitch values
and
the pit depth are stable and that no recalibration is needed, provided
that
we are careful in handling and using the specimen. For example, any
nanodebris visible in the image is excluded from the measurement.

However, the quality consultant says that it is common to send such
samples
to an outside lab for recalibration. He added that we should either do
recalibration or write a justification for why recalibration is not
necessary.



I am curious what other people do about this. One can consider this in
several different environments:

-the AFM or SEM manufacturer, who wants to provide good calibration for
the
instruments

-the AFM or SEM user who is supporting a production or QC application

-the AFM or SEM user who is mainly supporting R&D, but needs to show
traceability in order to meet the requirements of a quality management
system.



Here are a few practical questions to start the discussion.

If you have such a standard recalibrated, what is the time interval?

If you do not recalibrate the standard, what rationale do you give for
why
it is unnecessary?

Can you articulate the reasons so that it is clear they are based on
good
science and engineering data and practices?

Do you have any other comments on this subject?



regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes,
consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================



==============================Original Headers==============================
35, 37 -- From wesaia-at-iastate.edu Fri May 8 10:29:37 2009
35, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
35, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48FTZlq019909
35, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:29:36 -0500
35, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
35, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48FTRwP013689;
35, 37 -- Fri, 8 May 2009 10:29:27 -0500
35, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
35, 37 -- id 3a8b_0486950c_3be5_11de_bbf4_00137253420a;
35, 37 -- Fri, 08 May 2009 10:29:27 -0500
35, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
35, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n48FTQep022370;
35, 37 -- Fri, 8 May 2009 10:29:26 -0500
35, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
35, 37 -- Fri, 8 May 2009 10:29:26 -0500
35, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
35, 37 -- Content-class: urn:content-classes:message
35, 37 -- MIME-Version: 1.0
35, 37 -- Content-Type: text/plain;
35, 37 -- charset="us-ascii"
35, 37 -- Subject: RE: [Microscopy] Recalibration of AFM and SEM Calibration Standards
35, 37 -- Date: Fri, 8 May 2009 10:30:26 -0500
35, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE723E-at-maire.eng.iastate.edu}
35, 37 -- In-Reply-To: {200905072001.n47K11mR025823-at-ns.microscopy.com}
35, 37 -- X-MS-Has-Attach:
35, 37 -- X-MS-TNEF-Correlator:
35, 37 -- Thread-Topic: [Microscopy] Recalibration of AFM and SEM Calibration Standards
35, 37 -- Thread-Index: AcnPTo/GoVCN8eZBS/ePYCdGH9/lsQAm6MdQ
35, 37 -- References: {200905072001.n47K11mR025823-at-ns.microscopy.com}
35, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
35, 37 -- To: {Microscopy-at-microscopy.com}
35, 37 -- Cc: {donc-at-asmicro.com}
35, 37 -- X-OriginalArrivalTime: 08 May 2009 15:29:26.0780 (UTC) FILETIME=[C5B4D3C0:01C9CFF1]
35, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.151924
35, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
35, 37 -- Content-Transfer-Encoding: 8bit
35, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48FTZlq019909
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Fri, 8 May 2009 10:54:01 -0500
Subject: [Microscopy] Recalibration of AFM and SEM Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Warren and Don,

No one has mentioned what quality system is being used for certification.
My guess is it's an ISO system, but exactly which system it is has to be
known. ISO 2001 and 2008 do not mandate a time frame for calibration.
rather it is up to the company that has the equipment to determine what is
a reasonable time frame for re-calibration. I'm not familiar with all
other quality systems, but it would state in the standard how calibration
has to be handeled.

I have scales for weight I have recalibrated every 4 months. I have gage
blocks I re-calibrate every 3 years. I have optical calibration standards
I never re-calibrate (essentially, I listed the calibration time period as
50 years, way more than I'll ever need to worry about). These later would
be the equivalent to the standard orginally asked about. These are a
non-contact calibration standard and were recalibrated once a number of
years ago after having been in use for about 30 years. Since they were in
calibration at the time of recalibration - the material (as in your case)
does not degrade over time - the usage is non-contact - I have the
historical record showing that over 3 decades they never lost calibration
- I can then say they don't need re-calibration for another 50 years to
the ISO auditor. They don't like it and have said to me that I should
reconsider that and at least re-calibrate every 10 years. But, I am in
compliance.

Essentially, it is the historical records that determine the time frame
for re-calibration. Kind of a chicken and egg scenario, with the initial
time frame chosen through best guess. Then if the standard is never out of
calibration over a couple of re-calibrations, then you can justify
widening the time period.

As an FYI, the auditors that I have dealt with in fact do get
"recalibrated" ... :)

dj

On Fri, 8 May 2009, wesaia-at-iastate.edu wrote:

} Who (re)calibrates these quality auditors? What certification do they
} have? It seems like this auditor is out of their element.
}
} Maybe I am a little flip to think that the burden of proof should go the
} other way. We could assume that a standard would still be valid unless
} it has been demonstrated that it can go out of calibration over some
} period of time. Frankly, I cannot understand why someone would think a
} piece of silicon would change dimension on that scale because it sat on
} a shelf for 10 years. I think diffusion is a little slower than that.
}
} Disclaimer: Our lab has come under such rigorous, externally supervised
} quality control. We use our experience to check the quality of our own
} work. I daresay we do as well, and maybe better, than some labs that
} pass the auditor's review.
}
} Warren S.
}
} -----Original Message-----
} X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com]
} Sent: Thursday, May 07, 2009 3:01 PM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Recalibration of AFM and SEM Calibration Standards
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} My message today is motivated by questions asked by a quality systems
} auditor who recently visited my lab.
}
}
}
} We calibrate the XYZ scan axes of our AFM using a traceable height and
} pitch
} standard made of thermally grown silicon dioxide on silicon. The
} specimen
} contains these patterns:
}
} 10 um pitch, 2-dimensional array of pits, nominally 200 nm deep
}
} 2 um pitch, 1-dimensional array of ridges, same depth as the pits.
}
}
}
} The manufacturer's certificate of traceability does not discuss
} recalibration or indicate any need for it.
}
} Based on the materials of construction, I believe that the pitch values
} and
} the pit depth are stable and that no recalibration is needed, provided
} that
} we are careful in handling and using the specimen. For example, any
} nanodebris visible in the image is excluded from the measurement.
}
} However, the quality consultant says that it is common to send such
} samples
} to an outside lab for recalibration. He added that we should either do
} recalibration or write a justification for why recalibration is not
} necessary.
}
}
}
} I am curious what other people do about this. One can consider this in
} several different environments:
}
} -the AFM or SEM manufacturer, who wants to provide good calibration for
} the
} instruments
}
} -the AFM or SEM user who is supporting a production or QC application
}
} -the AFM or SEM user who is mainly supporting R&D, but needs to show
} traceability in order to meet the requirements of a quality management
} system.
}
}
}
} Here are a few practical questions to start the discussion.
}
} If you have such a standard recalibrated, what is the time interval?
}
} If you do not recalibrate the standard, what rationale do you give for
} why
} it is unnecessary?
}
} Can you articulate the reasons so that it is clear they are based on
} good
} science and engineering data and practices?
}
} Do you have any other comments on this subject?
}
}
}
} regards,
} Don
} =============================================
} Don Chernoff, Ph.D., President
} Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
} 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
} INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
} Canada)
} web: http://www.asmicro.com Fax: 317-895-5652
} [business activities: analytical services in AFM, AFM probes,
} consulting,
} training,
} calibration and test specimens, calibration and measurement software,
} used NanoScope equipment.]
} =============================================
}
}
}
} ==============================Original Headers==============================
} 35, 37 -- From wesaia-at-iastate.edu Fri May 8 10:29:37 2009
} 35, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
} 35, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48FTZlq019909
} 35, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:29:36 -0500
} 35, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
} 35, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48FTRwP013689;
} 35, 37 -- Fri, 8 May 2009 10:29:27 -0500
} 35, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
} 35, 37 -- id 3a8b_0486950c_3be5_11de_bbf4_00137253420a;
} 35, 37 -- Fri, 08 May 2009 10:29:27 -0500
} 35, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
} 35, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n48FTQep022370;
} 35, 37 -- Fri, 8 May 2009 10:29:26 -0500
} 35, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 35, 37 -- Fri, 8 May 2009 10:29:26 -0500
} 35, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 35, 37 -- Content-class: urn:content-classes:message
} 35, 37 -- MIME-Version: 1.0
} 35, 37 -- Content-Type: text/plain;
} 35, 37 -- charset="us-ascii"
} 35, 37 -- Subject: RE: [Microscopy] Recalibration of AFM and SEM Calibration Standards
} 35, 37 -- Date: Fri, 8 May 2009 10:30:26 -0500
} 35, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE723E-at-maire.eng.iastate.edu}
} 35, 37 -- In-Reply-To: {200905072001.n47K11mR025823-at-ns.microscopy.com}
} 35, 37 -- X-MS-Has-Attach:
} 35, 37 -- X-MS-TNEF-Correlator:
} 35, 37 -- Thread-Topic: [Microscopy] Recalibration of AFM and SEM Calibration Standards
} 35, 37 -- Thread-Index: AcnPTo/GoVCN8eZBS/ePYCdGH9/lsQAm6MdQ
} 35, 37 -- References: {200905072001.n47K11mR025823-at-ns.microscopy.com}
} 35, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 35, 37 -- To: {Microscopy-at-microscopy.com}
} 35, 37 -- Cc: {donc-at-asmicro.com}
} 35, 37 -- X-OriginalArrivalTime: 08 May 2009 15:29:26.0780 (UTC) FILETIME=[C5B4D3C0:01C9CFF1]
} 35, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.151924
} 35, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
} 35, 37 -- Content-Transfer-Encoding: 8bit
} 35, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48FTZlq019909
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
8, 19 -- From dljones-at-bestweb.net Fri May 8 10:54:01 2009
8, 19 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48Fs09A002783
8, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:54:00 -0500
8, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202])
8, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 19 -- (No client certificate requested)
8, 19 -- by smtp3.bestweb.net (Postfix) with ESMTPS id 242E85CD3
8, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 11:53:59 -0400 (EDT)
8, 19 -- Date: Fri, 8 May 2009 15:19:49 +0000 (UTC)
8, 19 -- From: dljones {dljones-at-bestweb.net}
8, 19 -- To: microscopy-at-microscopy.com
8, 19 -- Subject: Re: [Microscopy] RE: Recalibration of AFM and SEM Calibration
8, 19 -- Standards
8, 19 -- In-Reply-To: {200905081533.n48FXN8j025242-at-ns.microscopy.com}
8, 19 -- Message-ID: {Pine.BSF.4.64.0905081502200.75218-at-monet.bestweb.net}
8, 19 -- References: {200905081533.n48FXN8j025242-at-ns.microscopy.com}
8, 19 -- MIME-Version: 1.0
8, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 8 May 2009 12:45:38 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:

} I do not recall the name/type of the tubing
} that I used but it was milky white in color, not very flexible and
} about 8mm
} in diameter. Someone on the List will most likely tell us the name.


Dear Pat,
Two of the more likely possibilities are polyethylene and teflon.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Fri May 8 12:45:38 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48HjbOd022034
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 12:45:37 -0500
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 75C0832803E
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:45:37 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 6C343328081
6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:45:36 -0700 (PDT)
6, 22 -- Message-Id: {6CA1162D-742F-4FB5-81B1-DC2504BD5763-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200905081504.n48F4SP0004749-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak
6, 22 -- Date: Fri, 8 May 2009 10:45:33 -0700
6, 22 -- References: {200905081504.n48F4SP0004749-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Fri, 8 May 2009 16:10:02 -0500
Subject: [Microscopy] Re: viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Elgin;
My I suggest also that you have created a significant safety hazard by
running your air table, and I presume your valving, on nitrogen. The
leak you now have (and everything develops leaks eventually) is
filling up your room with pure nitrogen and displacing the oxygen. You
should be using only clean dry air for these items so that when it
leaks, the room fills up with.....air! Air that you can breath!
Nitrogen should only be used for venting the chamber.


Sent from my iPhone.
John Mardinly

On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---
} ---
} ---------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both eawoodruff-at-live.com as well as the MIcroscopy
} Listserver
} ---
} ---
} ---------------------------------------------------------------------
}
} Email: eawoodruff-at-live.com
} Name: Elvin Woodruff III
}
} Organization: Tennessee State University
}
} Title-Subject: [Filtered] Nitrogen leak
}
} Question: Hi All,
}
} I have recently started working with a JEOL 6701F scanning electron
} microscopy. The scope is running just fine, but it is going through
} nitrogen gas like there is not tomorrow. The scope sits on an air
} table which is supplied by gas (could this be it?)and other areas
} such as the specimen load chamber is also filled with nitrogen gas. I
} have talked to a couple of other people about the gas loss but to no
} avail. I have checked all of the usual places for a leak but can't
} find anything. We don't have a service contract right now so any help
} or ideas on why we are going through so much gas would be a big help.
}
} Thanks
}
} Elvin
}
} Login Host: 68.19.196.245
} ---
} ---
} ---------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 9, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:41:46 2009
} 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22
} ])
} 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n48CfjvE003327
} 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009
} 07:41:46 -0500
} 9, 11 -- Mime-Version: 1.0
} 9, 11 -- Message-Id: {p06240801c629d8f35379-at-[206.69.208.22]}
} 9, 11 -- Date: Fri, 8 May 2009 07:41:44 -0500
} 9, 11 -- To: microscopy-at-microscopy.com
} 9, 11 -- From: eawoodruff-at-live.com (by way of MicroscopyListserver)
} 9, 11 -- Subject: viaWWW: Nitrogen leak
} 9, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
5, 43 -- From jmardinly-at-gmail.com Fri May 8 16:10:01 2009
5, 43 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.180])
5, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48L9w3w012003
5, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:10:00 -0500
5, 43 -- Received: by wa-out-1112.google.com with SMTP id j5so1559172wah.2
5, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 08 May 2009 14:09:57 -0700 (PDT)
5, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 43 -- d=gmail.com; s=gamma;
5, 43 -- h=domainkey-signature:received:received:references:message-id:from:to
5, 43 -- :in-reply-to:content-type:content-transfer-encoding:x-mailer
5, 43 -- :mime-version:subject:date:cc;
5, 43 -- bh=T81jJPsbh63+1v0E7EZDETjfrX6ctFwpHfOzfK/FUc8=;
5, 43 -- b=j7iNmmBSCPKdzWDBfPWD+ilL4k+1/Asb1WWir4cI6+iKcIl+zU4pM2vr+3aK4L5Sr6
5, 43 -- ABRXIUI5tzj9XAhWxlMzerCkMaUfMX4qZuL0vZCcQ+cTuQ5/iGmpSGc4lsMfiAMJFfHj
5, 43 -- zdx/Ctu2feGJivrmWWB1vxo2j9naAax1UGgRQ=
5, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 43 -- d=gmail.com; s=gamma;
5, 43 -- h=references:message-id:from:to:in-reply-to:content-type
5, 43 -- :content-transfer-encoding:x-mailer:mime-version:subject:date:cc;
5, 43 -- b=YfvD8rZcFHuozdmTWLzU4tVfMymUcmx8jgh1w8etKgvLnD9edAflL+SRDqthhXIHiM
5, 43 -- oc11COWiIa4/nxt2aCFmeK0OEQqybBiV6T1RJNhQM0oOdpcSk6FSph2zh2eB83iaMT2G
5, 43 -- cVUvoRGBXRYMGtbRuc5HtrWqeuhBxUm7B4yq4=
5, 43 -- Received: by 10.114.81.1 with SMTP id e1mr3656009wab.136.1241816997893;
5, 43 -- Fri, 08 May 2009 14:09:57 -0700 (PDT)
5, 43 -- Received: from ?192.168.1.68? (75-25-131-32.lightspeed.sjcpca.sbcglobal.net [75.25.131.32])
5, 43 -- by mx.google.com with ESMTPS id k14sm1848845waf.65.2009.05.08.14.09.55
5, 43 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
5, 43 -- Fri, 08 May 2009 14:09:56 -0700 (PDT)
5, 43 -- References: {200905081252.n48CqWGS030709-at-ns.microscopy.com}
5, 43 -- Message-Id: {0B58D47F-4A81-45D6-8E91-83A4D7B0A900-at-gmail.com}
5, 43 -- From: John Mardinly {jmardinly-at-gmail.com}
5, 43 -- To: "eawoodruff-at-live.com" {eawoodruff-at-live.com}
5, 43 -- In-Reply-To: {200905081252.n48CqWGS030709-at-ns.microscopy.com}
5, 43 -- Content-Type: text/plain;
5, 43 -- charset=us-ascii;
5, 43 -- format=flowed;
5, 43 -- delsp=yes
5, 43 -- Content-Transfer-Encoding: 7bit
5, 43 -- X-Mailer: iPhone Mail (5H11)
5, 43 -- Mime-Version: 1.0 (iPhone Mail 5H11)
5, 43 -- Subject: Re: [Microscopy] viaWWW: Nitrogen leak
5, 43 -- Date: Fri, 8 May 2009 14:09:50 -0700
5, 43 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Fri, 8 May 2009 16:17:54 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suspect polyethylene. It sounds a lot like the PEX (polyethylene
cross-linked) pipe used in home domestic water applications. Teflon
would probably be too flexible. It would be too expensive for the
thickness required to stand the pressure.

Warren S.

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Friday, May 08, 2009 12:47 PM
To: wesaia-at-iastate.edu


On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:

} I do not recall the name/type of the tubing
} that I used but it was milky white in color, not very flexible and
} about 8mm
} in diameter. Someone on the List will most likely tell us the name.


Dear Pat,
Two of the more likely possibilities are polyethylene and
teflon.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



==============================Original Headers==============================
14, 36 -- From wesaia-at-iastate.edu Fri May 8 16:17:53 2009
14, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
14, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48LHqDc017138
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:53 -0500
14, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
14, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48LHpof018415
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:51 -0500
14, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
14, 36 -- id 2486_b0b46180_3c15_11de_ad46_001372578af6;
14, 36 -- Fri, 08 May 2009 16:17:52 -0500
14, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
14, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n48LHotq014983
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:50 -0500
14, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 36 -- Fri, 8 May 2009 16:17:50 -0500
14, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 36 -- Content-class: urn:content-classes:message
14, 36 -- MIME-Version: 1.0
14, 36 -- Content-Type: text/plain;
14, 36 -- charset="us-ascii"
14, 36 -- Subject: RE: [Microscopy] viaWWW: Nitrogen leak
14, 36 -- Date: Fri, 8 May 2009 16:18:50 -0500
14, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE7301-at-maire.eng.iastate.edu}
14, 36 -- In-Reply-To: {200905081746.n48HkUJH023088-at-ns.microscopy.com}
14, 36 -- X-MS-Has-Attach:
14, 36 -- X-MS-TNEF-Correlator:
14, 36 -- Thread-Topic: [Microscopy] viaWWW: Nitrogen leak
14, 36 -- Thread-Index: AcnQBOy16Wcj62O3QJObB5esGN629AAG5Mkg
14, 36 -- References: {200905081746.n48HkUJH023088-at-ns.microscopy.com}
14, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
14, 36 -- To: {Microscopy-at-microscopy.com}
14, 36 -- X-OriginalArrivalTime: 08 May 2009 21:17:50.0944 (UTC) FILETIME=[718F1E00:01C9D022]
14, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.210440
14, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
14, 36 -- Content-Transfer-Encoding: 8bit
14, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48LHqDc017138
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 8 May 2009 17:01:48 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In the past regular PE (not the cross-linked PEX) was commonly used for both
water and air. If there was a situation that had elevated temperature or
pressure, then nylon was the alternative. Polyurethane has been commonly
used for water, also.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: Friday, May 08, 2009 5:20 PM
To: kenconverse-at-qualityimages.biz

I would suspect polyethylene. It sounds a lot like the PEX (polyethylene
cross-linked) pipe used in home domestic water applications. Teflon
would probably be too flexible. It would be too expensive for the
thickness required to stand the pressure.

Warren S.

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Friday, May 08, 2009 12:47 PM
To: wesaia-at-iastate.edu


On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:

} I do not recall the name/type of the tubing
} that I used but it was milky white in color, not very flexible and
} about 8mm
} in diameter. Someone on the List will most likely tell us the name.


Dear Pat,
Two of the more likely possibilities are polyethylene and
teflon.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



==============================Original Headers==============================
14, 36 -- From wesaia-at-iastate.edu Fri May 8 16:17:53 2009
14, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu
[129.186.140.15])
14, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n48LHqDc017138
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:53
-0500
14, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu
[129.186.1.48])
14, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with
SMTP id n48LHpof018415
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:51
-0500
14, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by
devirus-11.iastate.edu with smtp
14, 36 -- id 2486_b0b46180_3c15_11de_ad46_001372578af6;
14, 36 -- Fri, 08 May 2009 16:17:52 -0500
14, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
[129.186.23.85])
14, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with
ESMTP id n48LHotq014983
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:50
-0500
14, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 36 -- Fri, 8 May 2009 16:17:50 -0500
14, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 36 -- Content-class: urn:content-classes:message
14, 36 -- MIME-Version: 1.0
14, 36 -- Content-Type: text/plain;
14, 36 -- charset="us-ascii"
14, 36 -- Subject: RE: [Microscopy] viaWWW: Nitrogen leak
14, 36 -- Date: Fri, 8 May 2009 16:18:50 -0500
14, 36 -- Message-ID:
{16A330AC32056A40B32842EC4BB8D72703DE7301-at-maire.eng.iastate.edu}
14, 36 -- In-Reply-To: {200905081746.n48HkUJH023088-at-ns.microscopy.com}
14, 36 -- X-MS-Has-Attach:
14, 36 -- X-MS-TNEF-Correlator:
14, 36 -- Thread-Topic: [Microscopy] viaWWW: Nitrogen leak
14, 36 -- Thread-Index: AcnQBOy16Wcj62O3QJObB5esGN629AAG5Mkg
14, 36 -- References: {200905081746.n48HkUJH023088-at-ns.microscopy.com}
14, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
14, 36 -- To: {Microscopy-at-microscopy.com}
14, 36 -- X-OriginalArrivalTime: 08 May 2009 21:17:50.0944 (UTC)
FILETIME=[718F1E00:01C9D022]
14, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912,
Antispam-Data: 2009.5.8.210440
14, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
Report='BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0,
BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0,
__C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0,
__CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0,
__MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
14, 36 -- Content-Transfer-Encoding: 8bit
14, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n48LHqDc017138
==============================End of - Headers==============================



==============================Original Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Fri May 8 17:01:47 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48M1jRf009521
25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 17:01:46 -0500
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20090508220143359.OQQU17201-at-cdptpa-omta01.mail.rr.com} ;
25, 25 -- Fri, 8 May 2009 22:01:43 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: {wesaia-at-iastate.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] RE: viaWWW: Nitrogen leak
25, 25 -- Date: Fri, 8 May 2009 18:01:36 -0400
25, 25 -- Message-ID: {920E986ABC46420688CF6FC4A0CDD472-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="us-ascii"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- In-Reply-To: {200905082119.n48LJquT021902-at-ns.microscopy.com}
25, 25 -- Importance: Normal
25, 25 -- Thread-Index: AcnQIr3i0yfXasasR3G907pI6f786wABVAnw
25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48M1jRf009521
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Fri, 8 May 2009 17:47:33 -0500
Subject: [Microscopy] PFM online discussion - Nanoprobe Network Announcement - May 8th

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

1. The Nanoprobe Network is hosting our third on-line Live Forum on
***Friday, May 22 from 11:30 AM to 1:00 PM, Eastern Daylight Time*.**
The discussion topic will be “*Piezoresponse Force Microscopy*”. You are
invited to post comments on the Nanoprobe Network Forum anytime prior
to, during, or after the event (http://nanoprobenetwork.org/forum).
During the Live Forum, AFM experts from around the globe will discuss
issues, ideas, and questions regarding the applications, principles,
recent advances and technical know-how of piezoresponse force microscopy
of ferroelectrics, multiferroics, and biological systems. The questions
you will be able to ask can range from:

* how can I implement PFM on a microsope
* how do I distinguish the PFM image from cross-talk
* how to acquire high-resolution spectroscopic data , and how to
interpret it
* what are the advantages and pitfalls of resonance enhanced PFM
* can PFM be done in liquid
* and many others.

Special expert guests for this chat will be Dr. Sergei Kalinin from Oak
Ridge National Laboratory, Dr. Andrei Kholkin from the University of
Aveiro, Portugal, and Dr. Alexei Gruverman from the University of
Nebraska. (http://nanoprobenetwork.org/bbpress/forum.php?id=26)

This text-based forum allows any Nanoprobe Network member to ask and
answer questions, propose ideas, suggest literature, and explore any
topic of interest related to PFM. To participate, you must be a member
of the Nanoprobe Network. Registration is free. Simply go to
http://www.nanoprobenetwork.org, and click on the "Register" button in
the upper right.

2. Enter the Network’s Best SPM Image Contest! The submission deadline
is Friday, May 29, and the user who submits the winning image will
receive an iPod Nano©. The winner of our first contest, to choose a
slogan for the Network, was Dr. Jan-Willem Weener from SmartTip. His
winning slogan entry is: "Think small, look deeper." Dr. Weener’s iPod
Nano© prize was sponsored by RHK Technology (http://www.rhk-tech.com/).
You can win the next iPod Nano by visiting
http://nanoprobenetwork.org/av-center/av-center-submission-instructions.

We hope you find the Nanoprobe Network resource beneficial to your work
and we look forward to seeing you on-line!

Cordially,
Hong-Mei Li
Nanoprobe Network Manager
http://nanoprobenetwork.org

P.S. *_Recent Posts_*

May 7, 2009
Live Forum Series: Piezoresponse Force Microscopy
http://nanoprobenetwork.org/bbpress/forum.php?id=26

May 6, 2009
Veeco AFM Basic Training Course with the Innova AFM
http://nanoprobenetwork.org/veeco-afm-basic-training-course-with-the-innova-afm

Veeco AFM Webinar Series: Advances AFM Applications Using the New
Dimension Icon
http://nanoprobenetwork.org/veeco-afm-webinar-series-advances-afm-applications-using-the-new-dimension-icon

--
Hong-Mei Li
Nanoprobe Network Administrator
Nano/Bio Interface Center (NBIC)
University of Pennsylvania

215-746-3210
nnadmin-at-seas.upenn.edu
www.nanoprobenetwork.org

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
18, 26 -- From sergei2-at-ornl.gov Fri May 8 17:47:33 2009
18, 26 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110])
18, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48MlWXN024852
18, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 17:47:32 -0500
18, 26 -- Received: from emroute3.ornl.gov ([127.0.0.1])
18, 26 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
18, 26 -- with ESMTP id {0KJC00620KN7OT-at-emroute3.ornl.gov} for
18, 26 -- microscopy-at-microscopy.com; Fri, 08 May 2009 18:47:32 -0400 (EDT)
18, 26 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov
18, 26 -- (PMDF V6.4 #31561) id {0KJC00701KN702-at-emroute3.ornl.gov} ; Fri,
18, 26 -- 08 May 2009 18:47:31 -0400 (EDT)
18, 26 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
18, 26 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
18, 26 -- with ESMTP id {0KJC005AQKN7PK-at-emroute3.ornl.gov} ; Fri,
18, 26 -- 08 May 2009 18:47:31 -0400 (EDT)
18, 26 -- Date: Fri, 08 May 2009 18:47:31 -0400
18, 26 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
18, 26 -- Subject: PFM online discussion - Nanoprobe Network Announcement - May 8th
18, 26 -- To: microscopy-at-microscopy.com
18, 26 -- Cc: "Robert W. Carpick" {carpick-at-seas.upenn.edu} ,
18, 26 -- "Dawn A. Bonnell" {bonnell-at-lrsm.upenn.edu}
18, 26 -- Message-id: {4A04B683.3070300-at-ornl.gov}
18, 26 -- MIME-version: 1.0
18, 26 -- Content-type: text/plain; charset=windows-1252; format=flowed
18, 26 -- Content-transfer-encoding: 8BIT
18, 26 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
==============================End of - Headers==============================




From: mlibbee-at-gmail.com
Date: Fri, 8 May 2009 18:18:07 -0500
Subject: [Microscopy] viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mlibbee-at-gmail.com
Name: Marissa Libbee

Title-Subject: [Filtered] Polishing Plexiglass

Question: Greetings!

I need to polish scratched plexiglass. Does anyone have any
experience with plexiglass prep? Any information on what abrasives
to use or which suspensions to avoid?

Thanks!

Login Host: 209.3.42.11
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri May 8 18:18:07 2009
7, 11 -- Received: from [130.202.238.72] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48NI3Gh007468
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:18:05 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c62a6e1c420e-at-[130.202.238.72]}
7, 11 -- Date: Fri, 8 May 2009 18:18:08 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Polishing Plexiglass
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Fri, 8 May 2009 19:19:12 -0500
Subject: [Microscopy] Which Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Group, again:

Thanks to those who gave me some good feedback about
the 570 and 4700. One suggestion was to call Hitachi
sales and ask about the legacy of systems and if
anyone was upgrading. No one seems to be upgrading at
this time. But I did come up with two suggested systems
that seem to fit my budget. I would appreciate feedback
on these, pro and con. I would like to find a system
in my budget range (~$20K) that has DP or turbo, type II
stage, specimen interlock and has all manuals--operation,
maintenance, schematics, and bakeout items. Some have
suggested using dry roughing pumps instead of rotary vane
pumps due to backstreaming and fouling the EDS window.

S-2700 is the last LaB6 Hitachi produced.

S-4500 is cir 1991 and was the first to have in-lens/TLD dual
detectors.

S-4200 has one SE.

The S-4700 is way over budget and the S-570 is unsupportable
due to lack of spare parts--especially V1.

All input is appreciated. I'm not in a hurry to buy a system,
but I would like to know what to be on the lookout for. I can
add Quartz PCI digital imaging capture or use the EDS scan
generator. But the SEM has to have an EDS port, conical lens,
and be user friendly for self-service to a maximum extent.

gary g.


==============================Original Headers==============================
9, 17 -- From gary-at-gaugler.com Fri May 8 19:19:12 2009
9, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n490JAwn024561
9, 17 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 19:19:11 -0500
9, 17 -- Message-Id: {200905090019.n490JAwn024561-at-ns.microscopy.com}
9, 17 -- Received: (qmail 779 invoked from network); 8 May 2009 17:13:20 -0700
9, 17 -- Received: by simscan 1.1.0 ppid: 776, pid: 777, t: 0.0806s
9, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 17 -- by smtp2 with SMTP; 8 May 2009 17:13:20 -0700
9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 17 -- Date: Fri, 08 May 2009 17:18:56 -0700
9, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
9, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 17 -- Subject: Which Hitachi SEM
9, 17 -- Mime-Version: 1.0
9, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Fri, 8 May 2009 19:20:24 -0500
Subject: [Microscopy] viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marissa,
There is an application note on our website for polishing polystyrene disks,
entitled, " Optical Polishing of Polystyrene Discs to Specific Dimensions".
The link is
http://www.southbaytech.com/appnotes/26%20Optical%20Polishing%20of%20Polysty
rene%20Discs%20to%20Specific%20Dimensions.PDF

It may be a little overkill for what you need, but it should be similar to
what you need to do or at least to get you started.

Disclaimer: SBT manufactures and sells lapping and polishing equipment and
consumables for materials processing.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: mlibbee-at-gmail.com [mailto:mlibbee-at-gmail.com]
Sent: Friday, May 08, 2009 4:28 PM
To: swalck-at-southbaytech.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mlibbee-at-gmail.com
Name: Marissa Libbee

Title-Subject: [Filtered] Polishing Plexiglass

Question: Greetings!

I need to polish scratched plexiglass. Does anyone have any
experience with plexiglass prep? Any information on what abrasives
to use or which suspensions to avoid?

Thanks!

Login Host: 209.3.42.11
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri May 8 18:18:07 2009
7, 11 -- Received: from [130.202.238.72] (msdvpn072.msd.anl.gov
[130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n48NI3Gh007468
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:18:05
-0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c62a6e1c420e-at-[130.202.238.72]}
7, 11 -- Date: Fri, 8 May 2009 18:18:08 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Polishing Plexiglass
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================
Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
5:29 PM




==============================Original Headers==============================
20, 30 -- From swalck-at-southbaytech.com Fri May 8 19:20:24 2009
20, 30 -- Received: from n15.bullet.mail.mud.yahoo.com (n15.bullet.mail.mud.yahoo.com [68.142.206.42])
20, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n490KMxG025205
20, 30 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 19:20:23 -0500
20, 30 -- Received: from [68.142.200.221] by n15.bullet.mail.mud.yahoo.com with NNFMP; 09 May 2009 00:20:20 -0000
20, 30 -- Received: from [68.142.201.252] by t9.bullet.mud.yahoo.com with NNFMP; 09 May 2009 00:20:20 -0000
20, 30 -- Received: from [127.0.0.1] by omp413.mail.mud.yahoo.com with NNFMP; 09 May 2009 00:20:20 -0000
20, 30 -- X-Yahoo-Newman-Id: 466258.43433.bm-at-omp413.mail.mud.yahoo.com
20, 30 -- Received: (qmail 50686 invoked from network); 9 May 2009 00:20:20 -0000
20, 30 -- Received: from unknown (HELO dynamicbl8uno3) (swalck-at-99.154.21.201 with login)
20, 30 -- by smtp119.plus.mail.sp1.yahoo.com with SMTP; 9 May 2009 00:20:19 -0000
20, 30 -- X-YMail-OSG: BPiA.XsVM1m07CKasqAMnAcvzJLtg0M8CIfRowAL2eRXiwzjptlpMzsgMGzujI4amxvuCimaAKZ0cenaqyhhUkJgD_b5mcdnr5don.klx7cryDqB3EmVFbiBpe.lSmknGPw35XOjfwmcdrw7iqzNv6.DxRVBV1xZVUAJk10FT1q51tXVQOzupLJEoNeDFlM8FXALxZSpmBXMG6cEB4jYog860HDhccpa1rmrjjsD.KlRdEcaHG8LvA8z67_iRkuovLoecdpoxGBmpH0fsNhZpVgX8ezkBENI0E_zpRacr24D3A9u398-
20, 30 -- X-Yahoo-Newman-Property: ymail-3
20, 30 -- Reply-To: {SWalck-at-southbaytech.com}
20, 30 -- From: "Scott Walck" {swalck-at-southbaytech.com}
20, 30 -- To: {Microscopy-at-microscopy.com}
20, 30 -- Cc: {mlibbee-at-gmail.com}
20, 30 -- References: {200905082327.n48NRYKx022005-at-ns.microscopy.com}
20, 30 -- In-Reply-To: {200905082327.n48NRYKx022005-at-ns.microscopy.com}
20, 30 -- Subject: RE: [Microscopy] viaWWW: Polishing Plexiglass
20, 30 -- Date: Fri, 8 May 2009 17:20:41 -0700
20, 30 -- Message-ID: {005701c9d03b$fca8f970$f5faec50$-at-com}
20, 30 -- MIME-Version: 1.0
20, 30 -- Content-Type: text/plain;
20, 30 -- charset="iso-8859-1"
20, 30 -- X-Mailer: Microsoft Office Outlook 12.0
20, 30 -- Thread-Index: AcnQNJHQr75bpPz8RC6MSeo6H10GLgABplSQ
20, 30 -- Content-Language: en-us
20, 30 -- Content-Transfer-Encoding: 8bit
20, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n490KMxG025205
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Sun, 10 May 2009 18:20:54 -0500
Subject: [Microscopy] Re: viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
I am trying to email Nigel, but the address I got from the MSA
directory bounced; could anyone send me a working eaddress for him?
TIA.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
2, 20 -- From tivol-at-caltech.edu Fri May 8 20:11:20 2009
2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n491BKgO021563
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 20:11:20 -0500
2, 20 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
2, 20 -- by earth-doxen-postvirus (Postfix) with ESMTP id 481C366E437D
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:11:20 -0700 (PDT)
2, 20 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
2, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
2, 20 -- by earth-doxen-ssl (Postfix) with ESMTP id 6FC5666E42BD
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:11:19 -0700 (PDT)
2, 20 -- Message-Id: {3DA1D736-75A7-4A45-8789-667D304FEF52-at-caltech.edu}
2, 20 -- From: Bill Tivol {tivol-at-caltech.edu}
2, 20 -- To: microscopy-at-microscopy.com
2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
2, 20 -- Content-Transfer-Encoding: 7bit
2, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3)
2, 20 -- Subject: Email address for Nigel Unwin
2, 20 -- Date: Fri, 8 May 2009 18:11:18 -0700
2, 20 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================

From misty-at-cybersource.com Sun May 10 14:08:23 2009
Return-Path: {misty-at-cybersource.com}
Received: from google.com (cpc3-basf6-0-0-cust955.nott.cable.ntl.com [86.1.191.188])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4AJ8KYH006253
for {microscopylistserverarchive-at-microscopy.com} ; Sun, 10 May 2009 14:08:22 -0500
Received: from [114.198.81.154] (HELO google.com)
by cleanranked.org; Sun, 10 May 2009 20:10:44 +0100
Message-ID: {000000040D0724C963243905}
Reply-To: Rexanne Baum {audrea.sivils14212-at-gmail.com}

Hi, Marissa

Here in New Zealand there is a brass polish on the market called
"Brasso" which can remove fine scratching and produce a fine polish. I
imagine there are similar products in other countries.

If the scratches are deeper than Brasso can remove, you can use really
fine emery paper (800 or even 1200) then polish with Brasso.

But try just the Brasso first, as you should use the emery paper only
if you need to. If you do have to use the emery paper, you may be
horrified at what it does, however, with Brasso and lots of rubbing
(with a soft cloth) you will get there. You may even conjure up a
genie, although I never have.

cheers
Ritchie Sims
Geology
University of Auckland



} Email: mlibbee-at-gmail.com
} Name: Marissa Libbee
}
} Title-Subject: [Filtered] Polishing Plexiglass
}
} Question: Greetings!
}
} I need to polish scratched plexiglass. Does anyone have any
} experience with plexiglass prep? Any information on what abrasives
} to use or which suspensions to avoid?
}
} Thanks!
}


----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.


==============================Original Headers==============================
11, 42 -- From r.sims-at-auckland.ac.nz Sun May 10 18:20:53 2009
11, 42 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ANKlH5016089
11, 42 -- for {microscopy-at-microscopy.com} ; Sun, 10 May 2009 18:20:52 -0500
11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1])
11, 42 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 80655668141;
11, 42 -- Mon, 11 May 2009 11:20:46 +1200 (NZST)
11, 42 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
11, 42 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
11, 42 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
11, 42 -- with ESMTP id FN-h5D4LgJjL; Mon, 11 May 2009 11:20:46 +1200 (NZST)
11, 42 -- Received: from uxchorprd02.its.auckland.ac.nz (uxchorprd02.its.auckland.ac.nz [130.216.10.23])
11, 42 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
11, 42 -- (No client certificate requested)
11, 42 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 5EBCA668140;
11, 42 -- Mon, 11 May 2009 11:20:44 +1200 (NZST)
11, 42 -- Received: from uxchorprd02.its.auckland.ac.nz (localhost.localdomain [127.0.0.1])
11, 42 -- by uxchorprd02.its.auckland.ac.nz (8.13.8/8.13.8) with ESMTP id n4ANKhYD013697;
11, 42 -- Mon, 11 May 2009 11:20:43 +1200
11, 42 -- Received: (from apache-at-localhost)
11, 42 -- by uxchorprd02.its.auckland.ac.nz (8.13.8/8.13.8/Submit) id n4ANKfIv013694;
11, 42 -- Mon, 11 May 2009 11:20:41 +1200
11, 42 -- X-Authentication-Warning: uxchorprd02.its.auckland.ac.nz: apache set sender to r.sims-at-auckland.ac.nz using -f
11, 42 -- Received: from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz
11, 42 -- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with HTTP;
11, 42 -- Mon, 11 May 2009 11:20:40 +1200
11, 42 -- Message-ID: {20090511112040.17906jqc4jdjus8w-at-webmail.auckland.ac.nz}
11, 42 -- Date: Mon, 11 May 2009 11:20:40 +1200
11, 42 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz}
11, 42 -- To: microscopy-at-microscopy.com
11, 42 -- Cc: mlibbee-at-gmail.com
11, 42 -- Subject: Re: [Microscopy] viaWWW: Polishing Plexiglass
11, 42 -- References: {200905082319.n48NJMSW008874-at-ns.microscopy.com}
11, 42 -- In-Reply-To: {200905082319.n48NJMSW008874-at-ns.microscopy.com}
11, 42 -- MIME-Version: 1.0
11, 42 -- Content-Type: text/plain;
11, 42 -- charset=ISO-8859-1;
11, 42 -- DelSp="Yes";
11, 42 -- format="flowed"
11, 42 -- Content-Disposition: inline
11, 42 -- Content-Transfer-Encoding: 7bit
11, 42 -- User-Agent: Internet Messaging Program (IMP) 4.3.3
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Mon, 11 May 2009 09:54:58 -0500
Subject: [Microscopy] RE: viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marissa,

Depending on how deep the scratches are, I've had lots of success using
a standard auto headlight polishing kit (Meguiar's Headlight and Clear
Plastic Restoration Kit) but I do all the sanding and polishing by hand
- it's too easy to ruin something using a drill. Note: I've done this to
restore appearance and visual clarity only, if you need to capture
images through the plexiglass, you'll need precision at a level far
greater than you'll ever achieve by hand.

I have no connection to this company other than as a satisfied customer

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.
================================================

Email: mlibbee-at-gmail.com
Name: Marissa Libbee

Title-Subject: [Filtered] Polishing Plexiglass

Question: Greetings!

I need to polish scratched plexiglass. Does anyone have any
experience with plexiglass prep? Any information on what abrasives
to use or which suspensions to avoid?

Thanks!


This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
14, 34 -- From Robert.Zonis-at-Sanford.com Mon May 11 09:54:58 2009
14, 34 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243])
14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4BEsvnO014442
14, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 09:54:57 -0500
14, 34 -- X-VirusChecked: Checked
14, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
14, 34 -- X-Msg-Ref: server-4.tower-192.messagelabs.com!1242053696!31680748!2
14, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
14, 34 -- X-Originating-IP: [198.176.16.25]
14, 34 -- Received: (qmail 10504 invoked from network); 11 May 2009 14:54:57 -0000
14, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
14, 34 -- by server-4.tower-192.messagelabs.com with SMTP; 11 May 2009 14:54:57 -0000
14, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
14, 34 -- Mon, 11 May 2009 09:54:57 -0500
14, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 34 -- Content-class: urn:content-classes:message
14, 34 -- MIME-Version: 1.0
14, 34 -- Content-Type: text/plain;
14, 34 -- charset="us-ascii"
14, 34 -- Subject: RE: [Microscopy] viaWWW: Polishing Plexiglass
14, 34 -- Date: Mon, 11 May 2009 09:52:09 -0500
14, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402AD79D1-at-naoaksasebe02.nr.ad.newellco.com}
14, 34 -- In-Reply-To: {200905082324.n48NOgGB016573-at-ns.microscopy.com}
14, 34 -- X-MS-Has-Attach:
14, 34 -- X-MS-TNEF-Correlator:
14, 34 -- Thread-Topic: [Microscopy] viaWWW: Polishing Plexiglass
14, 34 -- thread-index: AcnQNCx+6NHas5cASxmV4n0uQq27TgCEp2xA
14, 34 -- References: {200905082324.n48NOgGB016573-at-ns.microscopy.com}
14, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
14, 34 -- To: {mlibbee-at-gmail.com}
14, 34 -- Cc: {Microscopy-at-microscopy.com}
14, 34 -- X-OriginalArrivalTime: 11 May 2009 14:54:57.0064 (UTC) FILETIME=[734C7A80:01C9D248]
14, 34 -- Content-Transfer-Encoding: 8bit
14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4BEsvnO014442
==============================End of - Headers==============================




From: delannoy-at-jhmi.edu
Date: Mon, 11 May 2009 12:39:10 -0500
Subject: [Microscopy] morphometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Users,
I have a user who wants some morphometric data on his mitochondria.
He wants to prove if they are like tubules or round (ball-like) and he needs a good reference source (textbook or article). Basically wants 3d info without having to do reconstructions. Any ideas?

Thanks,
M Delannoy

==============================Original Headers==============================
2, 27 -- From delannoy-at-jhmi.edu Mon May 11 12:39:09 2009
2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4BHd9Wl004741
2, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:39:09 -0500
2, 27 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400";
2, 27 -- d="scan'208";a="211798483"
2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 May 2009 13:39:09 -0400
2, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu
2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006))
2, 27 -- with ESMTP id {0KJH00BM9QDAZF50-at-jesmail.johnshopkins.edu} for
2, 27 -- microscopy-at-microscopy.com; Mon, 11 May 2009 13:39:10 -0400 (EDT)
2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59])
2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 11 May 2009 13:39:10 -0400
2, 27 -- Date: Mon, 11 May 2009 13:39:10 -0400
2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
2, 27 -- Subject: morphometry
2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 27 -- Message-id: {f524aec09dac.4a082a7e-at-johnshopkins.edu}
2, 27 -- MIME-version: 1.0
2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006)
2, 27 -- Content-type: text/plain; charset=us-ascii
2, 27 -- Content-language: en
2, 27 -- Content-transfer-encoding: 7BIT
2, 27 -- Content-disposition: inline
2, 27 -- X-Accept-Language: en
2, 27 -- Priority: normal
==============================End of - Headers==============================




From: shishkov-at-HELIX.MGH.HARVARD.EDU
Date: Mon, 11 May 2009 12:55:30 -0500
Subject: [Microscopy] Re: viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

google "novus polish"

mlibbee-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: mlibbee-at-gmail.com
} Name: Marissa Libbee
}
} Title-Subject: [Filtered] Polishing Plexiglass
}
} Question: Greetings!
}
} I need to polish scratched plexiglass. Does anyone have any
} experience with plexiglass prep? Any information on what abrasives
} to use or which suspensions to avoid?
}
} Thanks!
}
} Login Host: 209.3.42.11
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Fri May 8 18:18:07 2009
} 7, 11 -- Received: from [130.202.238.72] (msdvpn072.msd.anl.gov [130.202.238.72])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48NI3Gh007468
} 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:18:05 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240802c62a6e1c420e-at-[130.202.238.72]}
} 7, 11 -- Date: Fri, 8 May 2009 18:18:08 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Polishing Plexiglass
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}


--
Milen Shishkov
Wellman Center for Photomedicine
Massachusetts General Hospital
Harvard Medical School

Address: MGH
BAR 712
50 Blossom St.
Boston, MA 02114
Phone: (617) 726 1589
Fax: (617) 726 4103



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.



==============================Original Headers==============================
9, 24 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Mon May 11 12:55:30 2009
9, 24 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65])
9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4BHtT86019746
9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:55:29 -0500
9, 24 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400";
9, 24 -- d="scan'208";a="80897428"
9, 24 -- Received: from phsxcon1.partners.org ([132.183.130.40])
9, 24 -- by phsmgmx11.partners.org with ESMTP; 11 May 2009 13:55:29 -0400
9, 24 -- Received: from [132.183.44.113] ([132.183.44.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959);
9, 24 -- Mon, 11 May 2009 13:55:29 -0400
9, 24 -- Message-ID: {4A08668D.5020405-at-helix.mgh.harvard.edu}
9, 24 -- Date: Mon, 11 May 2009 13:55:25 -0400
9, 24 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU}
9, 24 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
9, 24 -- MIME-Version: 1.0
9, 24 -- To: mlibbee-at-gmail.com
9, 24 -- CC: microscopy-at-microscopy.com
9, 24 -- Subject: Re: [Microscopy] viaWWW: Polishing Plexiglass
9, 24 -- References: {200905082319.n48NJkar009405-at-ns.microscopy.com}
9, 24 -- In-Reply-To: {200905082319.n48NJkar009405-at-ns.microscopy.com}
9, 24 -- X-OriginalArrivalTime: 11 May 2009 17:55:29.0486 (UTC) FILETIME=[ABECE6E0:01C9D261]
9, 24 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed"
9, 24 -- Content-Transfer-Encoding: 8bit
9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4BHtT86019746
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Mon, 11 May 2009 19:56:22 -0500
Subject: [Microscopy] viaWWW: TEM: JEOL 2200FS EELS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: David Mitchell

Organization: EMU, University of Sydney

Title-Subject: [Filtered] TEM: JEOL 2200FS EELS resolution

Question: Dear All

I am commissioning a standard JEOL2200FS with an omega filter, and am
looking at the EELS resolution. Routinely I can get 1.0eV resolution
(-at- 200kV) and if I throttle back A1 on the gun, I can get 0.9eV. I am
wondering if this is in the right ballpark, and if anyone is doing
much better, how are they doing it?

Thanks and regards

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 142A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


Login Host: 129.78.64.100
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:56:22 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0uKo2025265
14, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:56:21 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240803c62e79a49c97-at-[206.69.208.22]}
14, 11 -- Date: Mon, 11 May 2009 19:56:20 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: TEM: JEOL 2200FS EELS resolution
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nama1213-at-hotmail.com
Date: Mon, 11 May 2009 19:57:14 -0500
Subject: [Microscopy] viaWWW: AFM plant sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nama1213-at-hotmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nama1213-at-hotmail.com
Name: Naemah Al-Mansour

Organization: Kuwait University

Title-Subject: [Filtered] AFM sample preparation

Question: Hi

Does any one knows how to prepare plant samples for Atomic Force
Microscope (AFM). Basically I need to (1) measure roughness of leaf
surface and stomata, (2) prepare DNA for AFM and (3) capture images
of pollen grain (do I need to grind pollen into fine powder) and (4)
prpeare bacterial culture for AFM studies. I have tried some
techniques but would like to get help on published techniques that
anyone tried previously.

Many thanks and looking forward to hearing from you colleagues.

Regards
Naemah

Login Host: 78.89.1.123
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:57:13 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0vC47026048
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:57:13 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240804c62e79bda259-at-[206.69.208.22]}
9, 11 -- Date: Mon, 11 May 2009 19:57:11 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: nama1213-at-hotmail.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: AFM plant sample preparation
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jacqueline.williams-at-stjude.org
Date: Mon, 11 May 2009 19:57:40 -0500
Subject: [Microscopy] viaWWW: Disposal of sodium cacodylate 0.1M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jacqueline.williams-at-stjude.org as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jacqueline.williams-at-stjude.org
Name: jackie williams

Organization: St. Jude Children's Research Hospital

Title-Subject: [Filtered] Disposal of sodium cacodylate 0.1M

Question: There has been some discussions regarding the disposal of
sodium cacodylate. For numerous years we have disposed of the buffer
down the drain with copious amounts of water. Now there is questions
about the arsenic content. What are the current disposal rules?
Thanks in advance for any suggestions.

Login Host: 192.55.208.10
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:57:40 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0vdfY027377
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:57:40 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240805c62e79f1aeac-at-[206.69.208.22]}
6, 11 -- Date: Mon, 11 May 2009 19:57:38 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: jacqueline.williams-at-stjude.org (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Disposal of sodium cacodylate 0.1M
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cox_j-at-gwmail.gwu.edu
Date: Mon, 11 May 2009 19:58:20 -0500
Subject: [Microscopy] viaWWW: TEM Mag calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cox_j-at-gwmail.gwu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cox_j-at-gwmail.gwu.edu
Name: Jonathan Cox

Organization: The George Washington University

Title-Subject: [Filtered] TEM

Question: We recently had a digital camera installed on our TEM and
I'm currently calibration the various magnifications. Can anyone
point towards or send me an image of how to use catalased crystals
for calibrations of the higher magnifications? Thank you in advance.

Login Host: 128.164.239.33
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:58:20 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0wJ8l029783
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:58:20 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240806c62e7a0bb4cc-at-[206.69.208.22]}
6, 11 -- Date: Mon, 11 May 2009 19:58:18 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: cox_j-at-gwmail.gwu.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM Mag calibration
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Laabs-at-ameslab.gov
Date: Mon, 11 May 2009 20:02:38 -0500
Subject: [Microscopy] viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Laabs-at-ameslab.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Laabs-at-ameslab.gov
Name: Fran Laabs

Organization: Ames Lab, ISU

Title-Subject: [Filtered] Polishing Plexiglass

Question: This is in respose to Marissa's question regarding how to
polish Plexiglas.
There is a product called "Micromesh" sold by Wag-Aero
(www.wagaero.com). It is used to remove scratches from aircraft
windscreens and I can personally atest to the fact it works well.
Fran Laabs

Login Host: 147.155.184.124
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon May 11 20:02:38 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C12aRO012432
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 20:02:37 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240809c62e7b0af095-at-[206.69.208.22]}
6, 11 -- Date: Mon, 11 May 2009 20:02:35 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: Laabs-at-ameslab.gov (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Polishing Plexiglass
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Mon, 11 May 2009 21:52:41 -0500
Subject: [Microscopy] Re: viaWWW: TEM Mag calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jonathan,

Attached is my personal summary for image calibration with catalase
crystals.

(summary document not attached for the Listserver.. can send by request.
It is simply my notes with a scan of the image in the Wrigley paper....)
the text is below.

Catalase standard for magnification
==================================================================
Useful in the 10,000X to 200,000X range.

Nicholas G. Wrigley. The Lattice Spacing of Crystalline Catalase as an
Internal Standard of Length in Electron Microscopy. J. Ultrastructural
Research 24, 454-464. 1968.

The Wrigley paper describes recrystallization followed by glutaraldehyde
fixation to give thin platelet crystals that do not dissolve. The
spacing used for calibration is "1/2 Co"; there are a couple of values
given in the paper, 86 A or 87.5 A. This is the "obvious" line spacing
perpendicular to the long crystal axis (see image). Note that the image
is well filtered by reinforcement (old school method, pre computer) and
raw images do not look like this. There is a 68.5 A spacing parallel to
the long axis, Ao, that is less useful.

SPI sells catalase prepared by the Wrigley method. Others probably do
also. My bottle is from SPI and 15yr old and still good.

Apply the suspension to a glow-discharge treated carbon filmed grid and
allow to settle and adsorb until almost dry. Wick away the excess and
add a drop of 4% sodium silicotungstate, allowing 10-30s to penetrate,
and then wick excess and air dry the grid. 3% Ammonium molybdate and 2%
Uranyl Acetate are also used; Wrigley used the sodium silicotungstate.

Wrigley specifically states to NOT use the spacing at the edges of the
crystal since the spacing can be distorted. This image is from the
Wrigley paper.......

Image not included....

=============================================================

Method for prep.
The details are in the Wrigley paper. Make a suspension dilution in
distilled water that is slightly turbid and apply to hydrophilic
(glow-discharged) thin pure carbon films and negative stain with sodium
silico-tungstate as given in the Wrigley paper. Not every crystal will
be good and the staining will be better on some than others. Look at the
crystal edges where the crystal becomes more transparent. Look for areas
that show the lattice well and photograph at each mag needed. The images
can be measured and the calibration factors placed into the camera
software (consult your documentation - every camera is different).
Bottom line is that you will measure the spacings in the digital image
(measure multiple repeats for best accuracy) and that # of pixels is
equal to (spacing x #repeats) and that calibration is entered somewhere
(your camera software, the ImageJ plugin, etc.) I have a writeup on how
to have multiple calibrations (from different cameras for instance) in
an ImageJ plugin and choose on the fly.
http://www.bio.umass.edu/microscopy/resource.htm
and see the "Image Calibrations with ImageJ" section.

Hope this helps,

Dale Callaham
Umass Amherst


cox_j-at-gwmail.gwu.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both cox_j-at-gwmail.gwu.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: cox_j-at-gwmail.gwu.edu
} Name: Jonathan Cox
}
} Organization: The George Washington University
}
} Title-Subject: [Filtered] TEM
}
} Question: We recently had a digital camera installed on our TEM and
} I'm currently calibration the various magnifications. Can anyone
} point towards or send me an image of how to use catalased crystals
} for calibrations of the higher magnifications? Thank you in advance.
}
} Login Host: 128.164.239.33
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:58:20 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0wJ8l029783
} 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:58:20 -0500
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240806c62e7a0bb4cc-at-[206.69.208.22]}
} 6, 11 -- Date: Mon, 11 May 2009 19:58:18 -0500
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: cox_j-at-gwmail.gwu.edu (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM Mag calibration
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


==============================Original Headers==============================
17, 20 -- From dac-at-research.umass.edu Mon May 11 21:52:41 2009
17, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C2qe6b002157
17, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 21:52:40 -0500
17, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged))
17, 20 -- (authenticated bits=0)
17, 20 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4C2qdWM002589
17, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
17, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 22:52:40 -0400
17, 20 -- Message-ID: {4A08E47C.5070702-at-research.umass.edu}
17, 20 -- Date: Mon, 11 May 2009 22:52:44 -0400
17, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
17, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16
17, 20 -- MIME-Version: 1.0
17, 20 -- To: Microscopy-at-microscopy.com
17, 20 -- Subject: Re: [Microscopy] viaWWW: TEM Mag calibration
17, 20 -- References: {200905120107.n4C17icA030084-at-ns.microscopy.com}
17, 20 -- In-Reply-To: {200905120107.n4C17icA030084-at-ns.microscopy.com}
17, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
17, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Mon, 11 May 2009 22:05:21 -0500
Subject: [Microscopy] Re: viaWWW: TEM: JEOL 2200FS EELS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We got .7 to .6 on a 12 year old 2010F with a Tridiem at the highest
dispersion. I would think you should just as well with a 2200. You did
not indicate what dispersion you were using.
John Mardinly

Sent from my iPhone

On May 11, 2009, at 6:16 PM, david.mitchell-at-emu.usyd.edu.au wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---
} ---
} ---------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both david.mitchell-at-emu.usyd.edu.au as well as the
} MIcroscopy Listserver
} ---
} ---
} ---------------------------------------------------------------------
}
} Email: david.mitchell-at-emu.usyd.edu.au
} Name: David Mitchell
}
} Organization: EMU, University of Sydney
}
} Title-Subject: [Filtered] TEM: JEOL 2200FS EELS resolution
}
} Question: Dear All
}
} I am commissioning a standard JEOL2200FS with an omega filter, and am
} looking at the EELS resolution. Routinely I can get 1.0eV resolution
} (-at- 200kV) and if I throttle back A1 on the gun, I can get 0.9eV. I am
} wondering if this is in the right ballpark, and if anyone is doing
} much better, how are they doing it?
}
} Thanks and regards
}
} Dave Mitchell
}
}
} Dr David Mitchell
} Senior Microscopist, Transmission Electron Microscopy
}
} Contact:
} PH +61 2 9036 7633
} FAX +61 2 9351 7682
} David.mitchell-at-emu.usyd.edu.au
}
} Address:
} Electron Microscope Unit
} Australian Key Centre for Microscopy and Microanalysis
} Australian Microscopy & Microanalysis Research Facility (AMMRF)
} Madsen Building F09, Room 142A
} The University of Sydney
} NSW 2006, Australia
} www.emu.usyd.edu.au
} www.ammrf.org.au
}
}
} Login Host: 129.78.64.100
} ---
} ---
} ---------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 14, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:56:22 2009
} 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22
} ])
} 14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4C0uKo2025265
} 14, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009
} 19:56:21 -0500
} 14, 11 -- Mime-Version: 1.0
} 14, 11 -- Message-Id: {p06240803c62e79a49c97-at-[206.69.208.22]}
} 14, 11 -- Date: Mon, 11 May 2009 19:56:20 -0500
} 14, 11 -- To: microscopy-at-microscopy.com
} 14, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of
} MicroscopyListserver)
} 14, 11 -- Subject: viaWWW: TEM: JEOL 2200FS EELS resolution
} 14, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
4, 43 -- From jmardinly-at-gmail.com Mon May 11 22:05:21 2009
4, 43 -- Received: from mail-pz0-f128.google.com (mail-pz0-f128.google.com [209.85.222.128])
4, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C35KhI016862
4, 43 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 22:05:20 -0500
4, 43 -- Received: by pzk34 with SMTP id 34so64783pzk.10
4, 43 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 20:05:20 -0700 (PDT)
4, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=domainkey-signature:received:received:references:message-id:from:to
4, 43 -- :in-reply-to:content-type:content-transfer-encoding:x-mailer
4, 43 -- :mime-version:subject:date:cc;
4, 43 -- bh=Dkc91Wtb2i9qYUmD2/RYRVQVBrWd/nmU4Qe67b3rEAw=;
4, 43 -- b=oUoSr9hRfvaxr4q4e8EAebwwxyo5NeF7GymjmuXreNkpptUwzFxF1i/NW0MmW0O3le
4, 43 -- rdnVbSiGA2+Okd7CnRUubyqWe5Pfvs9wCgM7ThorxryS6xku/zvQ2n55+Qmt3v6+LQP+
4, 43 -- OrGX8OoGqQWyZ4YnnCikAcphLljH1IULAIQHE=
4, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=references:message-id:from:to:in-reply-to:content-type
4, 43 -- :content-transfer-encoding:x-mailer:mime-version:subject:date:cc;
4, 43 -- b=N+CTZK3+tIwHjei/a07/tx1kITADSpNSe+Ks2AZrO+Qpg3YWe7N5jPD8zWezABLDvf
4, 43 -- 8+zPOKjYNiZktdOgyY+t4MV0zrXG+Op3xl4Y9tYTNeKy4K+ePiOn2CpT7TSR0vc6Xx+P
4, 43 -- wERCoNwcuBTzAvxGtjz0fEuDNsWW1Rr3G4JVo=
4, 43 -- Received: by 10.115.58.18 with SMTP id l18mr6131919wak.31.1242097520186;
4, 43 -- Mon, 11 May 2009 20:05:20 -0700 (PDT)
4, 43 -- Received: from ?192.168.1.68? (75-25-131-32.lightspeed.sjcpca.sbcglobal.net [75.25.131.32])
4, 43 -- by mx.google.com with ESMTPS id d20sm6895231waa.47.2009.05.11.20.05.18
4, 43 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 43 -- Mon, 11 May 2009 20:05:19 -0700 (PDT)
4, 43 -- References: {200905120116.n4C1GJuC028471-at-ns.microscopy.com}
4, 43 -- Message-Id: {FF974636-9982-4A96-A0C2-1C2D0CE52618-at-gmail.com}
4, 43 -- From: John Mardinly {jmardinly-at-gmail.com}
4, 43 -- To: "david.mitchell-at-emu.usyd.edu.au" {david.mitchell-at-emu.usyd.edu.au}
4, 43 -- In-Reply-To: {200905120116.n4C1GJuC028471-at-ns.microscopy.com}
4, 43 -- Content-Type: text/plain;
4, 43 -- charset=us-ascii;
4, 43 -- format=flowed;
4, 43 -- delsp=yes
4, 43 -- Content-Transfer-Encoding: 7bit
4, 43 -- X-Mailer: iPhone Mail (5H11)
4, 43 -- Mime-Version: 1.0 (iPhone Mail 5H11)
4, 43 -- Subject: Re: [Microscopy] viaWWW: TEM: JEOL 2200FS EELS resolution
4, 43 -- Date: Mon, 11 May 2009 20:05:13 -0700
4, 43 -- Cc: MSA Listserver {microscopy-at-microscopy.com}
==============================End of - Headers==============================




From: takenomm-at-u.washington.edu
Date: Tue, 12 May 2009 00:02:57 -0500
Subject: [Microscopy] viaWWW: Polishing Plexiglass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

TAP Plastics, a US West Coast plastics company, specializes in Plexiglas
(AKA Perspex/Acrylite /acrylic, etc. for our non-US readers) (as well as
cast acrylic resin, fiberglass, and carbon fiber) materials for the home
hobbyist and other interested parties (such as scientists!). TAP's
website has good technical information.

http://www.tapplastics.com/

For polishing and finishing, you may want to look at the following: (.pdf)
http://www.tapplastics.com/uploads/pdf/Tech%20Data-Edge.pdf
http://www.tapplastics.com/uploads/pdf/a_working_acrylite.pdf

TAP has a video that you may find helpful:
http://www.tapplastics.com/info/video.php#video26

also posted on YouTube:
http://www.youtube.com/watch?v=4yp-kumgpoM

usual disclaimer: satisfied customer.

-------- Original Message --------

Email: mlibbee-at-gmail.com
Name: Marissa Libbee

Title-Subject: [Filtered] Polishing Plexiglass

Question: Greetings!

I need to polish scratched plexiglass. Does anyone have any
experience with plexiglass prep? Any information on what abrasives
to use or which suspensions to avoid?

Thanks!

Login Host: 209.3.42.11
---------------------------------------------------------------------------


--

Marc Takeno, Ph.D. | Research Scientist | University of Washington
Department of Bioengineering | N330B Foege | Box 355061
(206) 543-4290 | takenomm-at-u.washington.edu


==============================Original Headers==============================
17, 27 -- From takenomm-at-u.washington.edu Tue May 12 00:02:56 2009
17, 27 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19])
17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C52uXD006756
17, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 00:02:56 -0500
17, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.7] (may be forged))
17, 27 -- by mxout4.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) with ESMTP id n4C52tBW001224
17, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK);
17, 27 -- Mon, 11 May 2009 22:02:55 -0700
17, 27 -- X-Auth-Received: from [192.168.1.112] (75-172-97-226.tukw.qwest.net [75.172.97.226])
17, 27 -- (authenticated authid=takenomm)
17, 27 -- by smtp.washington.edu (8.14.3+UW09.03/8.14.3+UW09.01) with ESMTP id n4C52rQ5014019
17, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
17, 27 -- Mon, 11 May 2009 22:02:55 -0700
17, 27 -- Message-ID: {4A0902FE.4090104-at-u.washington.edu}
17, 27 -- Date: Mon, 11 May 2009 22:02:54 -0700
17, 27 -- From: Marc Takeno {takenomm-at-u.washington.edu}
17, 27 -- Organization: University of Washington
17, 27 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
17, 27 -- MIME-Version: 1.0
17, 27 -- To: microscopy-at-microscopy.com
17, 27 -- CC: mlibbee-at-gmail.com
17, 27 -- Subject: [Microscopy] viaWWW: Polishing Plexiglass
17, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
17, 27 -- Content-Transfer-Encoding: 7bit
17, 27 -- X-PMX-Version: 5.5.4.371499, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.5.12.45227
17, 27 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
17, 27 -- BODY_SIZE_1400_1499 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MOZILLA_MSGID 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0, __USER_AGENT 0'
==============================End of - Headers==============================




From: wim.vandenbroeck-at-UGent.be
Date: Tue, 12 May 2009 04:14:27 -0500
Subject: [Microscopy] SEM need help on Immunogold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues and friends,

I need to perform a SEM analysis of virus particles, attached to fibers, after immunogold labeling of the particles. However, I do not have any experience with immunogold and SEM. Please can you give me some advice, protocols, tips and trics, …

Kind regards,
Wim.

-------------------------------------------------------
Wim Van den Broeck, DVM, MSc, PhD
Department of Morphology
Laboratory of Cytology and Histology
Faculty of Veterinary Medicine, Ghent University
Salisburylaan 133, B-9820 Merelbeke,
BELGIUM
tel.: +32 (0)9 264 77 16
fax: +32 (0)9 264 77 90
Email: wim.vandenbroeck-at-UGent.be
-------------------------------------------------------
ï Please consider the environment before printing this e-mail. Please add this line to your email to tell others.






==============================Original Headers==============================
9, 36 -- From wim.vandenbroeck-at-UGent.be Tue May 12 04:14:27 2009
9, 36 -- Received: from ugmailsa.ugent.be (ugmailsa.ugent.be [157.193.49.116])
9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C9EQxY002180
9, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 04:14:26 -0500
9, 36 -- Received: from localhost (localhost [127.0.0.1])
9, 36 -- by ugmailsa.ugent.be (Postfix) with ESMTP id CBE4B306EB2
9, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 11:14:25 +0200 (CEST)
9, 36 -- X-Virus-Scanned: by UGent DICT
9, 36 -- Received: from ugmailsa.ugent.be ([127.0.0.1])
9, 36 -- by localhost (ugmailsa.ugent.be [127.0.0.1]) (amavisd-new, port 10024)
9, 36 -- with ESMTP id ftonvf4KCK7y for {Microscopy-at-microscopy.com} ;
9, 36 -- Tue, 12 May 2009 11:14:25 +0200 (CEST)
9, 36 -- Received: from cedar.ugent.be (cedar.ugent.be [157.193.49.14])
9, 36 -- by ugmailsa.ugent.be (Postfix) with ESMTP id 4DA6B306BD7
9, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 11:14:24 +0200 (CEST)
9, 36 -- X-IronPort-Anti-Spam-Filtered: true
9, 36 -- X-IronPort-Anti-Spam-Result: AsoEAHzaCEqdwbNY/2dsb2JhbACWA7YchAIF
9, 36 -- Received: from pc20di03.ugent.be (HELO PC20DI03) ([157.193.179.88])
9, 36 -- by smtps9.UGent.be with ESMTP; 12 May 2009 11:14:23 +0200
9, 36 -- Reply-To: {wim.vandenbroeck-at-UGent.be}
9, 36 -- From: "Prof. dr. Wim Van den Broeck" {wim.vandenbroeck-at-UGent.be}
9, 36 -- To: {Microscopy-at-microscopy.com}
9, 36 -- Subject: SEM need help on Immunogold labeling
9, 36 -- Date: Tue, 12 May 2009 11:14:44 +0200
9, 36 -- Organization: Universiteit Gent - Vakgroep Morfologie
9, 36 -- Message-ID: {001f01c9d2e2$1729ff80$457dfe80$-at-vandenbroeck-at-UGent.be}
9, 36 -- MIME-Version: 1.0
9, 36 -- Content-Type: text/plain;
9, 36 -- charset="UTF-8"
9, 36 -- X-Mailer: Microsoft Office Outlook 12.0
9, 36 -- Thread-Index: AcnS4hRc4G/BY85jSk6Ab7PxqFfs3w==
9, 36 -- Content-Language: nl-be
9, 36 -- x-cr-hashedpuzzle: AStP BgIN B3DF CR4Z CZAe Chja DLrv DYtp E41R FVRp FcKw F39/ HZ9c IRe3 JTvj KgWz;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{2814F0D0-FB1C-423F-8A8F-7C87BE2F5B0B};dwBpAG0ALgB2AGEAbgBkAGUAbgBiAHIAbwBlAGMAawBAAHUAZwBlAG4AdAAuAGIAZQA=;Tue, 12 May 2009 09:14:40 GMT;UwBFAE0AIABuAGUAZQBkACAAaABlAGwAcAAgAG8AbgAgAEkAbQBtAHUAbgBvAGcAbwBsAGQAIABsAGEAYgBlAGwAaQBuAGcA
9, 36 -- x-cr-puzzleid: {2814F0D0-FB1C-423F-8A8F-7C87BE2F5B0B}
9, 36 -- Content-Transfer-Encoding: 8bit
9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4C9EQxY002180
==============================End of - Headers==============================




From: ehaller-at-health.usf.edu
Date: Tue, 12 May 2009 07:28:27 -0500
Subject: [Microscopy] RE: Information About Repairing Specimen Tips--FEI Morgagni TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

I recently accepted a position in an E.M. lab with a Morgagni TEM. Previous users have damaged several of the specimen tips for the TEM, and I am writing the List to see if anyone has a connection with someone that repairs the tips. The tips are not the same size as those from 200 or 300 series TEM's from Philips. Thanks in advance for the input!

Ed Haller
University of South Florida
Integrative Biology
Tampa, FL 33620
813-974-2676




==============================Original Headers==============================
7, 35 -- From ehaller-at-health.usf.edu Tue May 12 07:28:27 2009
7, 35 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45])
7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CCSQPU029446
7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 07:28:27 -0500
7, 35 -- X-ASG-Debug-ID: 1242131303-57ec013b0000-4CH8be
7, 35 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi
7, 35 -- Received: from mailhub1.hscnet.hsc.usf.edu (localhost [127.0.0.1])
7, 35 -- by hscantispam.health.usf.edu (Spam Firewall) with ESMTP id 20DD44094D87
7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:28:23 -0400 (EDT)
7, 35 -- Received: from mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) by hscantispam.health.usf.edu with ESMTP id QQHQlhTfRBJoOcDU for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:28:23 -0400 (EDT)
7, 35 -- Received: from MAILSERVER2.hscnet.hsc.usf.edu ([10.119.4.17]) by
7, 35 -- mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) with mapi; Tue, 12 May 2009
7, 35 -- 08:28:24 -0400
7, 35 -- From: "Haller, Edward" {ehaller-at-health.usf.edu}
7, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
7, 35 -- Date: Tue, 12 May 2009 08:26:56 -0400
7, 35 -- X-ASG-Orig-Subj: RE: Information About Repairing Specimen Tips--FEI Morgagni TEM
7, 35 -- Subject: RE: Information About Repairing Specimen Tips--FEI Morgagni TEM
7, 35 -- Thread-Topic: Information About Repairing Specimen Tips--FEI Morgagni TEM
7, 35 -- Thread-Index: AcnOeLxmIBvIeUBJTgmLdQ+hBXyNLAEhDPCF
7, 35 -- Message-ID: {7D1470DAD69A6744AE69D98054321F340112A26E7746-at-MAILSERVER2.hscnet.hsc.usf.edu}
7, 35 -- References: {200905061830.n46IUQjX007432-at-ns.microscopy.com}
7, 35 -- In-Reply-To: {200905061830.n46IUQjX007432-at-ns.microscopy.com}
7, 35 -- Accept-Language: en-US
7, 35 -- Content-Language: en-US
7, 35 -- X-MS-Has-Attach:
7, 35 -- X-MS-TNEF-Correlator:
7, 35 -- acceptlanguage: en-US
7, 35 -- Content-Type: text/plain; charset="us-ascii"
7, 35 -- MIME-Version: 1.0
7, 35 -- X-Barracuda-Connect: UNKNOWN[10.119.4.12]
7, 35 -- X-Barracuda-Start-Time: 1242131304
7, 35 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu
7, 35 -- Content-Transfer-Encoding: 8bit
7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CCSQPU029446
==============================End of - Headers==============================




From: r.g.phillips-at-sussex.ac.uk
Date: Tue, 12 May 2009 07:58:16 -0500
Subject: [Microscopy] viaWWW: MRC studentships for MSc in Imaging in Biomedical Research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both r.g.phillips-at-sussex.ac.uk as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: r.g.phillips-at-sussex.ac.uk
Name: Roger Phillips

Organization: University of Sussex

Title-Subject: [Filtered] MRC studentships for MSc in Imaging in
Biomedical Research

Question: Dear Colleagues,
The University of Sussex MSc programme in 'Imaging in Biomedical
Research' has seven MRC-funded studentships on offer to UK and EU
students for study beginning this Autumn. The programme is designed
for good graduates in biological sciences who wish to acquire a
strong grounding in imaging including physical and mathematical
approaches before starting a research career. We have taken the
liberty of addressing this specifically to the Microscopy Listserver
as we hope that the programme will produce graduates who many of you
would find well-suited to starting a PhD in your laboratory. We would
be very grateful if you could forward this information to your
graduating class of students.

'Imaging in Biomedical Research' is a new interdisciplinary programme
in the Department of Biochemistry taught in collaboration with the
University of Sussex Departments of Physics and Astronomy and
Informatics and with the Brighton and Sussex Medical School Clinical
Imaging Science Centre and the Sussex Centre for Advanced Microscopy.
The programme provides a strong background for a career in research
in the academic, clinical and industrial sectors. The student is
taught theory, applications and practice in a broad range of imaging
techniques including Electron Microscopy, Fluorescence Microscopy,
Magnetic Resonance Imaging and Computed Tomography. Training in
mathematical computation and in digital imaging provides the basis
for understanding advanced processing and analyses and will include
hands-on experience with practical computing languages such as Matlab
and ImageJ. Students will benefit from access to both high content
and high throughput microscopy facilities which are essential tools
of pharmacological research and development. Options in the Autumn
and Spring terms allow each student to specialise in different areas
of biomedical imaging such as Molecular, Neuro or Developmental
Biology. In the Summer term each student will plan and execute a
research project under joint supervision of a member of faculty in
one of the collaborating Departments and staff of one of the imaging
centres.

All UK (home student) applications received by 17 July 2009 will be
considered for funding and MRC Studentships will be awarded to the
top applicants. EU students are eligible for tuition but not for
stipend on MRC studentships. See our prospectus
http://www.sussex.ac.uk/Units/publications/pgrad2009/areasofstudy/Biochemistry/22178
and the Centre for Advanced Microscopy website
http://www.sussex.ac.uk/lifesci/1-4-30.html
for further information.

Many thanks and best wishes,

Julian Thorpe
John Armstrong
Roger Phillips

University of Sussex



Login Host: 139.184.30.134
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Tue May 12 07:58:16 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CCwFiB012344
13, 11 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 07:58:15 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240801c62f22d44481-at-[206.69.208.22]}
13, 11 -- Date: Tue, 12 May 2009 07:58:14 -0500
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: r.g.phillips-at-sussex.ac.uk (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: MRC studentships for MSc in Imaging in Biomedical Research
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 12 May 2009 07:59:09 -0500
Subject: [Microscopy] SEM image problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for suggestions for a possible cause for a recently occurring
SEM image problem.

In the last 24 hours our JEOL JSM-5800 has developed a slow image flutter.
It's very slow and almost relaxing. It's not the jaggies you see from 60
cycle scan interference, nor is it the jumping you sometimes see with
charging. It seems more noticeable at 2kv as compared to 25kv, but only
just.

I'm examining polished copper in a conductive support media. I have in the
past done this and never experienced a problem. The problem doesn't seem
to be time dependent, I see the same problem at 4:15pm (most people have
turned off their equipment) and at 8:00am (everything is running full
tilt!)%

We are experiencing a new noise in out of our chiller, possible a minor
surge problem. I believe the chiller only cools the diffusion pump and not
any of the lens control circuits. I could be wrong about this.

Any suggestion would be welcome!

stay safe........


Frank



--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
13, 21 -- From frank_karl-at-lincolnelectric.com Tue May 12 07:59:09 2009
13, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CCx8OV013481
13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 07:59:08 -0500
13, 21 -- Subject: SEM image problem
13, 21 -- To: Microscopy-at-microscopy.com
13, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
13, 21 -- Message-ID: {OF8F1A06ED.1554F19D-ON852575B4.00460916-852575B4.00474D71-at-lincolnelectric.com}
13, 21 -- Date: Tue, 12 May 2009 08:58:48 -0400
13, 21 -- From: Frank_Karl-at-lincolnelectric.com
13, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM,
13, 21 -- CD-MIME complete at 05/12/2009 08:58:49 AM,
13, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM,
13, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM,
13, 21 -- Serialize complete at 05/12/2009 08:58:49 AM
13, 21 -- MIME-Version: 1.0
13, 21 -- Content-Type: text/plain;
13, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 12 May 2009 08:04:56 -0500
Subject: [Microscopy] Information About Repairing Specimen Tips--FEI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom Schmelzer has been doing a great job on our sample rods.

TGS Technologies, LLC.
702 Little Creek Lane
Cranberry Township PA 16066
USA
Ph: 724.453.3865
Fax: 724.453.2968
E-mail: tom-at-tgstechnologies.net
Website: http://www.tgstechnologies.net

Just a satisfied customer.

Cheers,
Henk


At 08:31 AM 05/12/09, ehaller-at-health.usf.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
11, 27 -- From colijn.1-at-osu.edu Tue May 12 08:04:56 2009
11, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CD4tok026671
11, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:04:56 -0500
11, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
11, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
11, 27 -- id {01N8UYABKCQO8ZWE58-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
11, 27 -- Tue, 12 May 2009 09:04:49 -0400 (EDT)
11, 27 -- Received: from HOC1.ecr6.ohio-state.edu
11, 27 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
11, 27 -- (PMDF V6.3-x18 #31556)
11, 27 -- with ESMTPA id {01N8UYA8H57K8Y2URF-at-ecr6.ohio-state.edu} ; Tue,
11, 27 -- 12 May 2009 09:04:43 -0400 (EDT)
11, 27 -- Date: Tue, 12 May 2009 09:06:02 -0400
11, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
11, 27 -- Subject: Re: [Microscopy] RE: Information About Repairing Specimen Tips--FEI
11, 27 -- Morgagni TEM
11, 27 -- In-reply-to: {200905121231.n4CCVMbE031684-at-ns.microscopy.com}
11, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
11, 27 -- To: ehaller-at-health.usf.edu
11, 27 -- Cc: Microscopy-at-microscopy.com
11, 27 -- Message-id: {01N8UYA8M45U8Y2URF-at-ecr6.ohio-state.edu}
11, 27 -- MIME-version: 1.0
11, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed
11, 27 -- X-Env-From: auth/colijn.1-at-osu.edu
11, 27 -- References: {200905121231.n4CCVMbE031684-at-ns.microscopy.com}
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Tue, 12 May 2009 08:32:45 -0500
Subject: [Microscopy] Re: viaWWW: Disposal of sodium cacodylate 0.1M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jackie-
Our Environmental Health Office insists that we collect all buffer
wastes for pick up and disposal by them. Everything must be
identified by content so that they can use the correct method.
Lee

} ---------------------------------------------------------------------------
}
} Email: jacqueline.williams-at-stjude.org
} Name: jackie williams
}
} Organization: St. Jude Children's Research Hospital
}
} Title-Subject: [Filtered] Disposal of sodium cacodylate 0.1M
}
} Question: There has been some discussions regarding the disposal of
} sodium cacodylate. For numerous years we have disposed of the buffer
} down the drain with copious amounts of water. Now there is questions
} about the arsenic content. What are the current disposal rules?
} Thanks in advance for any suggestions.
}
} Login Host: 192.55.208.10
} ---------------------------------------------------------------------------

--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
4, 34 -- From lcgould-at-med.cornell.edu Tue May 12 08:32:44 2009
4, 34 -- Received: from mail-gw1.med.cornell.edu (mail-gw1.med.cornell.edu [140.251.3.44])
4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CDWhnO022972
4, 34 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:32:44 -0500
4, 34 -- MIME-version: 1.0
4, 34 -- Content-transfer-encoding: 7BIT
4, 34 -- Content-type: text/plain; charset=us-ascii; format=flowed
4, 34 -- Received: from mpx6.med.cornell.edu ([140.251.11.120])
4, 34 -- by mail-gw1.med.cornell.edu
4, 34 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
4, 34 -- with ESMTP id {0KJJ00DVH9MIXO30-at-mail-gw1.med.cornell.edu} for
4, 34 -- microscopy-at-microscopy.com; Tue, 12 May 2009 09:32:42 -0400 (EDT)
4, 34 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
4, 34 -- by mpx6.med.cornell.edu
4, 34 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
4, 34 -- with ESMTPA id {0KJJ00JST9MG1U20-at-mpx6.med.cornell.edu} ; Tue,
4, 34 -- 12 May 2009 09:32:42 -0400 (EDT)
4, 34 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912,
4, 34 -- Antispam-Data: 2009.5.12.131933
4, 34 -- X-Perlmx-Spam: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1200_1299 0,
4, 34 -- BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
4, 34 -- TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_NAME_BODY 0,
4, 34 -- __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0,
4, 34 -- __HAS_MSGID 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
4, 34 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0'
4, 34 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
4, 34 -- Message-id: {p06230906c62f2a82606e-at-[140.251.48.23]}
4, 34 -- In-reply-to: {200905120113.n4C1Dpuw020269-at-ns.microscopy.com}
4, 34 -- References: {200905120113.n4C1Dpuw020269-at-ns.microscopy.com}
4, 34 -- Date: Tue, 12 May 2009 09:32:35 -0400
4, 34 -- To: jacqueline.williams-at-stjude.org,
4, 34 -- Microscopy Listserver {microscopy-at-microscopy.com}
4, 34 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
4, 34 -- Subject: Re: [Microscopy] viaWWW: Disposal of sodium cacodylate 0.1M
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Tue, 12 May 2009 08:49:57 -0500
Subject: [Microscopy] Re: viaWWW: Disposal of sodium cacodylate 0.1M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jackie

In spite of being a growth industry at our University, our Environmental
Health and Safety Office has had to be prodded on this particular
issue. They are still not sure how they should deal with UA solutions.
We do as Leona - bottle and record relative concentrations of buffers
with fixative, all but PBS without fixative (we do dump straight PBS and
Hanks), waste stain solutions, etc. This is then taken down for
disposal. The amounts are not great, and who knows what actually
happens with these things when they get there. After all, I still
remember the news story about the recycling program in the Toronto area
which was recycling by sending the material to a land fill near Buffalo,
or was it the other way around.

We are working on the assumption that someday EHSO will figure it out.
In the meantime we are doing our best to be safe and environmentally
responsible.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
7, 20 -- From paul_hazelton-at-umanitoba.ca Tue May 12 08:49:56 2009
7, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CDntmG005592
7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:49:55 -0500
7, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
7, 20 -- (authenticated bits=0)
7, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n4CDnrKM009824;
7, 20 -- Tue, 12 May 2009 08:49:53 -0500 (CDT)
7, 20 -- Message-ID: {4A097E78.6090602-at-umanitoba.ca}
7, 20 -- Date: Tue, 12 May 2009 08:49:44 -0500
7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
7, 20 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
7, 20 -- MIME-Version: 1.0
7, 20 -- To: jacqueline.williams-at-stjude.org
7, 20 -- Subject: Re: [Microscopy] viaWWW: Disposal of sodium cacodylate 0.1M
7, 20 -- References: {200905120100.n4C1012A003633-at-ns.microscopy.com}
7, 20 -- In-Reply-To: {200905120100.n4C1012A003633-at-ns.microscopy.com}
7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Tue, 12 May 2009 08:55:24 -0500
Subject: [Microscopy] Re: Disposal of sodium cacodylate 0.1M

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Jacqueline,

perhaps you've got meanwhile some personal Re's from Listers.
Since I have not seen any responses on the list I would like to recommend an OSHA-Document, assuming your location are the USA:
http://www.osha.gov/SLTC/healthguidelines/arsenic/recognition.html

Find: Hazardous waste management requirements:
"...Cacodylic acid is listed as a hazardous waste under RCRA and has been assigned EPA Hazardous Waste No. U136. This substance has been banned from land disposal."

Unfortunately I do not know about regulation of disposal of hydrous solutions in your particualr area and unfortunately I could not find any specific hint in the document above....

A similar problem I see within regulations for this Lab-waste in European regulatory rules.

So I collect all cacodylate buffer waste solutions into a bottle (needless to say that I keep the volume(s) as little as possible), and dispose of as "heavy metal containing" chemical waste solutions.

Perhaps this is - considering the minimal amount of As in the buffer - exaggerated .... but nevertheless in the limits of regulations for heavy metals assigned Toxic, hazardous and environmental pollutants.

Best regards,

Wolfgang MUSS
SALZBURG/AUSTRIA




} -----Ursprüngliche Nachricht-----
} Von: jacqueline.williams-at-stjude.org
} [mailto:jacqueline.williams-at-stjude.org]
} Gesendet: Dienstag, 12. Mai 2009 03:04
} An: Muß Wolfgang
} Betreff: [Microscopy] Disposal of sodium cacodylate 0.1M
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both jacqueline.williams-at-stjude.org as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
} Email: jacqueline.williams-at-stjude.org
} Name: jackie williams
} Organization: St. Jude Children's Research Hospital
} Title-Subject:
} Disposal of sodium cacodylate 0.1M
} Question:
}
}
} There has been some discussions regarding the disposal of
} sodium cacodylate.
} For numerous years we have disposed of the buffer down the
} drain with copious amounts of water. Now there is questions
} about the arsenic content. What are the current disposal rules?
} Thanks in advance for any suggestions.
}
}
}
} Login Host: 192.55.208.10
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:57:40 2009
} 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n4C0vdfY027377
} 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May
} 2009 19:57:40 -0500
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240805c62e79f1aeac-at-[206.69.208.22]}
} 6, 11 -- Date: Mon, 11 May 2009 19:57:38 -0500
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of
} MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: Disposal of sodium cacodylate 0.1M
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
16, 36 -- From W.Muss-at-salk.at Tue May 12 08:55:24 2009
16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CDtNPF012854
16, 36 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:55:23 -0500
16, 36 -- Received: from localhost (localhost [127.0.0.1])
16, 36 -- by hermes.salk.at (Postfix) with ESMTP id BB559C385B;
16, 36 -- Tue, 12 May 2009 15:55:20 +0200 (CEST)
16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
16, 36 -- Received: from hermes.salk.at ([127.0.0.1])
16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
16, 36 -- with ESMTP id qYAiuNg982Y1; Tue, 12 May 2009 15:55:20 +0200 (CEST)
16, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 4C0B6C3858;
16, 36 -- Tue, 12 May 2009 15:55:20 +0200 (CEST)
16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
16, 36 -- Tue, 12 May 2009 15:55:20 +0200
16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 36 -- Content-class: urn:content-classes:message
16, 36 -- MIME-Version: 1.0
16, 36 -- Content-Type: text/plain;
16, 36 -- charset="iso-8859-1"
16, 36 -- Subject: [Microscopy] Re: Disposal of sodium cacodylate 0.1M
16, 36 -- Date: Tue, 12 May 2009 15:55:20 +0200
16, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0A1E-at-N1RZ116.lksdom21.lks.local}
16, 36 -- In-Reply-To: {200905120103.n4C13VxW015674-at-ns.microscopy.com}
16, 36 -- X-MS-Has-Attach:
16, 36 -- X-MS-TNEF-Correlator:
16, 36 -- Thread-Topic: [Microscopy] Re: Disposal of sodium cacodylate 0.1M
16, 36 -- Thread-Index: AcnSnX8G9aSAFydaSj+zwIwZgRKw+AAaafGA
16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
16, 36 -- To: {jacqueline.williams-at-stjude.org}
16, 36 -- Cc: {microscopy-at-microscopy.com}
16, 36 -- X-OriginalArrivalTime: 12 May 2009 13:55:20.0289 (UTC) FILETIME=[49C9AD10:01C9D309]
16, 36 -- X-Scanned-By: SALK-Content-Filter
16, 36 -- Content-Transfer-Encoding: 8bit
16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CDtNPF012854
==============================End of - Headers==============================




From: rjharris-at-uwo.ca
Date: Tue, 12 May 2009 15:01:31 -0500
Subject: [Microscopy] morphometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jackie,

At NIH we send the Sodium Cacodylate Buffer as well as the fixatives made with it (each separately) through our Chemical Waste Disposal Department. I was told when I started using this buffer that the reason is the arsenic.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E - Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do
not represent the NIH. This message is not
confidential and can be freely shared and reproduced.
==

X-from: {jacqueline.williams-at-stjude.org}
Reply-To: {jacqueline.williams-at-stjude.org}

That sounds more like a stereology problem than a morphology one - try
Elais, Hans, Hyde, Dallas M.
A Guide to Practical Stereology 1983. S.Krager AG

Rick,

Richard Harris, Manager - Imaging and Data Systems
The Biotron - Experimental Climate Change Research
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph.  519-661-2111 ext. 86780
Fax  519-661-3935
e-mail rjharris-at-uwo.ca
web: www.biotron.uwo.ca

-----Original Message-----
X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu]
Sent: Monday, May 11, 2009 1:47 PM
To: rjharris-at-uwo.ca

Dear Users,
I have a user who wants some morphometric data on his mitochondria.
He wants to prove if they are like tubules or round (ball-like) and he needs
a good reference source (textbook or article). Basically wants 3d info
without having to do reconstructions. Any ideas?

Thanks,
M Delannoy

==============================Original Headers==============================
2, 27 -- From delannoy-at-jhmi.edu Mon May 11 12:39:09 2009
2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu
[162.129.8.141])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n4BHd9Wl004741
2, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:39:09
-0500
2, 27 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400";
2, 27 -- d="scan'208";a="211798483"
2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 May 2009
13:39:09 -0400
2, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by
jesmail.johnshopkins.edu
2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006))
2, 27 -- with ESMTP id {0KJH00BM9QDAZF50-at-jesmail.johnshopkins.edu} for
2, 27 -- microscopy-at-microscopy.com; Mon, 11 May 2009 13:39:10 -0400 (EDT)
2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59])
2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 11 May 2009 13:39:10
-0400
2, 27 -- Date: Mon, 11 May 2009 13:39:10 -0400
2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
2, 27 -- Subject: morphometry
2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 27 -- Message-id: {f524aec09dac.4a082a7e-at-johnshopkins.edu}
2, 27 -- MIME-version: 1.0
2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May
24 2006)
2, 27 -- Content-type: text/plain; charset=us-ascii
2, 27 -- Content-language: en
2, 27 -- Content-transfer-encoding: 7BIT
2, 27 -- Content-disposition: inline
2, 27 -- X-Accept-Language: en
2, 27 -- Priority: normal
==============================End of - Headers==============================



==============================Original Headers==============================
12, 29 -- From rjharris-at-uwo.ca Tue May 12 15:01:31 2009
12, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146])
12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CK1U8a005859
12, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:01:30 -0500
12, 29 -- MIME-version: 1.0
12, 29 -- Content-type: text/plain; charset=iso-8859-1
12, 29 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41])
12, 29 -- by zeppo.mail.uwo.pri
12, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
12, 29 -- with ESMTP id {0KJJ0071PRMG38G0-at-zeppo.mail.uwo.pri} for
12, 29 -- Microscopy-at-microscopy.com; Tue, 12 May 2009 16:01:28 -0400 (EDT)
12, 29 -- Received: from rjbook
12, 29 -- (CPE001839e498ad-CM0019475d354c.cpe.net.cable.rogers.com [99.249.82.51])
12, 29 -- by zeppo.mail.uwo.pri
12, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit))
12, 29 -- with ESMTPSA id {0KJJ00I6PRMFYE20-at-zeppo.mail.uwo.pri} for
12, 29 -- Microscopy-at-microscopy.com; Tue, 12 May 2009 16:01:28 -0400 (EDT)
12, 29 -- From: Richard Harris {rjharris-at-uwo.ca}
12, 29 -- To: delannoy-at-jhmi.edu, MSA Listserver {Microscopy-at-microscopy.com}
12, 29 -- References: {200905111746.n4BHku6j017215-at-ns.microscopy.com}
12, 29 -- In-reply-to: {200905111746.n4BHku6j017215-at-ns.microscopy.com}
12, 29 -- Subject: RE: [Microscopy] morphometry
12, 29 -- Date: Tue, 12 May 2009 16:01:28 -0400
12, 29 -- Message-id: {002d01c9d33c$701a9aa0$504fcfe0$-at-ca}
12, 29 -- X-Mailer: Microsoft Office Outlook 12.0
12, 29 -- Content-language: en-us
12, 29 -- Thread-index: AcnSYH1waPPOU5oWR+CKASsms06p2QA24SoA
12, 29 -- Content-Transfer-Encoding: 8bit
12, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CK1U8a005859
==============================End of - Headers==============================




From: trazy-at-umn.edu
Date: Tue, 12 May 2009 15:26:45 -0500
Subject: [Microscopy] Microwave Workshop May 20-22 - several spots have just opened up!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microwave Processing Workshop May 20-22, 2009
CBS Imaging Center, University of Minnesota

Several spots have opened up in our upcoming microwave workshop!  Click the
following link for more info or to register:

http://www.cbs.umn.edu/ic/events/mwws09.shtml

A workshop focusing on new techniques in microwave specimen processing for
light and electron microscopy.
• In vivo labeling
• Principles of microwave fixation for biological materials
• Principles of immunolocalization for:
• Confocal and fluorescence microscopy
• Electron microscopy
• In situ hybridization
As part of the Workshop we will be using our state-of-the-art wide field and
confocal microscope systems.

Workshop Information (PDF):

http://www.cbs.umn.edu/ic/events/MWWS2009_Handout.pdf



--
Tracy E. Anderson
Microscopist and digital imaging specialist 
Imaging Center
University of Minnesota
College of Biological Sciences
Phone:  612.624.3454
Fax:  612.624.1799
http://www.cbs.umn.edu/ic/
 




==============================Original Headers==============================
12, 22 -- From trazy-at-umn.edu Tue May 12 15:26:44 2009
12, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CKQhe2020819
12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:26:43 -0500
12, 22 -- Received: from universiy0lfra (x-160-94-173-210.cbs.umn.edu [160.94.173.210])
12, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP
12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:26:42 -0500 (CDT)
12, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-210.cbs.umn.edu [160.94.173.210] #+LO+TS+AU+HN
12, 22 -- X-Umn-Classification: local
12, 22 -- From: "Tracy E. Anderson" {trazy-at-umn.edu}
12, 22 -- To: {Microscopy-at-microscopy.com}
12, 22 -- Subject: Microwave Workshop May 20-22 - several spots have just opened up!
12, 22 -- Date: Tue, 12 May 2009 15:26:43 -0500
12, 22 -- Message-ID: {BA7BC89F76E04E78B98DBB41765D8406-at-universiy0lfra}
12, 22 -- MIME-Version: 1.0
12, 22 -- Content-Type: text/plain;
12, 22 -- charset="iso-8859-1"
12, 22 -- X-Mailer: Microsoft Office Outlook 11
12, 22 -- Thread-Index: AcnTP/ZvM0y3VBO+SQuev3JNLuUDjA==
12, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CKQhe2020819
==============================End of - Headers==============================




From: albin.amy-at-gmail.com
Date: Tue, 12 May 2009 15:44:57 -0500
Subject: [Microscopy] Reichert-Jung (Leica) Ultra-cut 701701 Manual Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I was just wondering if anyone out there knew how I could get my hands
on Reichert-Jung (Leica) Ultra-cut 701701 manual. I have tried
searching for a PDF etc online, but I cant seem to find anything. Any
leads would be appreciated. Thank you.
Sincerely
Amy Albin
EM Technician
University of Cincinnati Physicians

==============================Original Headers==============================
1, 31 -- From albin.amy-at-gmail.com Tue May 12 15:44:57 2009
1, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.146])
1, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CKiuNI002985
1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:44:57 -0500
1, 31 -- Received: by qw-out-1920.google.com with SMTP id 9so164645qwj.54
1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 13:44:55 -0700 (PDT)
1, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
1, 31 -- :from:to:content-type:content-transfer-encoding;
1, 31 -- bh=ajMWJL+c7VRC9eZP3EZv+ZMesK7etCDxR28b91Dbbvo=;
1, 31 -- b=quwqWLhJxdvj2ID9zdHewWl+EiBWf8eVeg+ZkB+W6VrN9hTkhVu+GVF0NIv6G1PjSs
1, 31 -- MaE5U/1F66DUXoUmE7c/v9o/rmMV1SSqChML8yN/bCVLkaqPd95HaE0yzVuoKVvI2POZ
1, 31 -- R+tZ7HxIw+Wu6FnbujXcyFa4aM9NdgNyoiZT8=
1, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
1, 31 -- d=gmail.com; s=gamma;
1, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
1, 31 -- :content-transfer-encoding;
1, 31 -- b=VYMH5Ksmy+v0qXuHnCmukU+nU6eSinhKecfg80fattW93MgihhoC39tv4Uf69GBZbV
1, 31 -- ZmdoigjSn9pla34HLD1EMMGcLyqp+ah8aWT6mARKmID37O9HZAvNV7b26RCXyWHnV6K9
1, 31 -- EUV25G7mUYXxJ8lz2BAf/0JOaF2NQE9kYc9NM=
1, 31 -- MIME-Version: 1.0
1, 31 -- Received: by 10.229.109.202 with SMTP id k10mr163753qcp.58.1242161095384; Tue,
1, 31 -- 12 May 2009 13:44:55 -0700 (PDT)
1, 31 -- Date: Tue, 12 May 2009 16:44:55 -0400
1, 31 -- Message-ID: {32af5a0c0905121344o78e4e19bi72bda5a3b4a3bfea-at-mail.gmail.com}
1, 31 -- Subject: Reichert-Jung (Leica) Ultra-cut 701701 Manual Needed
1, 31 -- From: Amy Albin {albin.amy-at-gmail.com}
1, 31 -- To: Microscopy-at-microscopy.com
1, 31 -- Content-Type: text/plain; charset=ISO-8859-1
1, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: schaffne-at-patho.unibe.ch
Date: Tue, 12 May 2009 19:52:44 -0500
Subject: [Microscopy] viaWWW: optical staining of colorless living Organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both schaffne-at-patho.unibe.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: schaffne-at-patho.unibe.ch
Name: Thomas Schaffner

Organization: Institute of Pathology University of Bern

Title-Subject: [Filtered] Differential optical staining of colorless
living Organisms

Question: Hi, I wonder about an elegant technique shown at our
Institute about 35 years ago by R(obert) F Smith, then retired
photographer at the Brookhaven National Laboratory. He published an
article in J. Biol. Phot. Assoc Vol: 23, Nos 2 and 3, pages 74-77;
1955 entitled "Differential optical staining of colorless living
organisms in macrophotography", unfortunately unavailable through our
libraries.
The brilliant colored images of live fish embryos in sharply defined
z-planes were apparently obtained with polarized colored light
sources generating some kind of interference contrast if I am not
mistaken.
I now wonder about the physical principles that were involved and how
the stereoscope he usaed had to be set up.
Any comments are welcome.

Login Host: 130.92.9.55
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue May 12 19:52:43 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4D0qeSa024275
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 19:52:42 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c62fca478430-at-[206.69.208.22]}
6, 11 -- Date: Tue, 12 May 2009 19:52:40 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: schaffne-at-patho.unibe.ch (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: optical staining of colorless living Organisms
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 13 May 2009 08:23:34 -0500
Subject: [Microscopy] Re: viaWWW: optical staining of colorless living

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thomas,

This sounds like Rheinberg illumination. Basically, one sets up the
microscope for darkfield illumination, and then places colored
filters between the light source and the condenser. For example a
blue filter before 1/2 of the condenser, and an orange filter before
the other 1/2. Any color filters can be used, depending on the
desired effect. Darkfield illumination of whole critters
(gastrotrichs are really cool) can have a DIC look to them.
I haven't seen this used with crossed polarizers, but I don't see why
it can't be done.

Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
4, 24 -- From oshel1pe-at-cmich.edu Wed May 13 08:23:34 2009
4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DDNXFH000584
4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 08:23:34 -0500
4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 24 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n4DDNSWp027364
4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 09:23:30 -0400
4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 24 -- Wed, 13 May 2009 09:23:27 -0400
4, 24 -- Mime-Version: 1.0
4, 24 -- Message-Id: {f06240808c6307a3a167c-at-[141.209.160.249]}
4, 24 -- Date: Wed, 13 May 2009 09:23:24 -0400
4, 24 -- To: Microscopy-at-microscopy.com
4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 24 -- Subject: Re: [Microscopy] viaWWW: optical staining of colorless living
4, 24 -- Organisms
4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 24 -- X-OriginalArrivalTime: 13 May 2009 13:23:27.0894 (UTC) FILETIME=[00531B60:01C9D3CE]
4, 24 -- X-Canit-CHI2: 0.00
4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 24 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 24 -- X-CanItPRO-Stream: default
4, 24 -- X-Canit-Stats-ID: 13269997 - 07a5bab390cb
4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: hfong-at-u.washington.edu
Date: Wed, 13 May 2009 17:58:29 -0500
Subject: [Microscopy] viaWWW: Diffusion Pump for Gatan Duo 600 Ion Mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hfong-at-u.washington.edu as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: hfong-at-u.washington.edu
Name: Hanson Fong

Organization: Univ of Washington

Title-Subject: [Filtered] Diffusion Pump for Gatan Duo 600 Ion Mill

Question: Looking for a diffusion pump for our old Gatan Duo 600 ion mill.

Please contact Hanson Fong (hfong-at-u.washington.edu) if you have one
in good working condition to sell/donate.

Thank you.

Login Host: 128.95.118.88
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed May 13 17:58:29 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DMwRQI022175
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 13 May 2009 17:58:28 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c63101055a2e-at-[206.69.208.22]}
8, 11 -- Date: Wed, 13 May 2009 17:58:26 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: hfong-at-u.washington.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Diffusion Pump for Gatan Duo 600 Ion Mill
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: levilr-at-ptd.net
Date: Wed, 13 May 2009 17:59:22 -0500
Subject: [Microscopy] viaWWW: Gold Sputter Target Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both levilr-at-ptd.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: levilr-at-ptd.net
Name: LeeLevine

Organization: Process Solutions Consulting

Title-Subject: [Filtered] Gold Sputter Target Available

Question: 100% gold annular target on an aluminum ring , Fits Polaron
- BioRad - VG Microtech models E5100,SC515, SC510, SC7610, 82mmOD X
60mmID, 0.1 mm thickness

Brand new, never used, in a Ted Pella plastic box


Login Host: 173.49.248.73
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed May 13 17:59:21 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DMxKwK022912
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 13 May 2009 17:59:21 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c631011c5f8b-at-[206.69.208.22]}
8, 11 -- Date: Wed, 13 May 2009 17:59:19 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: levilr-at-ptd.net (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Gold Sputter Target Available
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 14 May 2009 09:56:01 -0500
Subject: [Microscopy] viaWWW: optical staining of colorless living

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

I seem to remember an interesting article in Microscopy today explaining the advantage of illuminating a sample with one wavelength. One could sequentially illuminate with 3 different wavelengthes and then merge the 3 images to obtain a sharper final image.
In my brain of biologist the explanation had something to do with the fact that a light containing different wavelengthes (like in white light) interfere in different planes (although very near).
Sorry if my explanation seems confuse, it probably reflects my understanding of the phenomena ;-)

Regards,

Stephane


} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

==============================Original Headers==============================
4, 24 -- From oshel1pe-at-cmich.edu Wed May 13 08:23:34 2009
4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 24 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DDNXFH000584
4, 24 --     for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 08:23:34 -0500
4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 24 --     by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n4DDNSWp027364
4, 24 --     for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 09:23:30 -0400
4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 24 --     Wed, 13 May 2009 09:23:27 -0400
4, 24 -- Mime-Version: 1.0
4, 24 -- Message-Id: {f06240808c6307a3a167c-at-[141.209.160.249]}
4, 24 -- Date: Wed, 13 May 2009 09:23:24 -0400
4, 24 -- To: Microscopy-at-microscopy.com
4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 24 -- Subject:  Re: [Microscopy] viaWWW: optical staining of colorless living
4, 24 --  Organisms
4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 24 -- X-OriginalArrivalTime: 13 May 2009 13:23:27.0894 (UTC) FILETIME=[00531B60:01C9D3CE]
4, 24 -- X-Canit-CHI2: 0.00
4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 24 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 24 -- X-CanItPRO-Stream: default
4, 24 -- X-Canit-Stats-ID: 13269997 - 07a5bab390cb
4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================






==============================Original Headers==============================
12, 24 -- From nizets2-at-yahoo.com Thu May 14 09:56:01 2009
12, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4EEtwrW026724
12, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 09:55:59 -0500
12, 24 -- Received: (qmail 60072 invoked by uid 60001); 14 May 2009 14:55:56 -0000
12, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1242312956; bh=3RJfD8Povpg0DJVJdklASkg/s9yTEUF8HfQbpUQVwsk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fozIxk6OdXBV6PUyiRNAj8+ch1NgIw3dWXUIzCbPAEGQixj33YjX55scMhwyEUZT9xvb1qv+8+Z8an4MmOcTEkAlTOigdLp91foqDi57u5GpFrOr63qw91P/F7G/j0rQ2vNEDy3g5xjZSIWOcKXvJgNfuv4NyiQ2LNp7rUrnRSc=
12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 24 -- s=s1024; d=yahoo.com;
12, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
12, 24 -- b=slhNGtyryROKrx/+Ehb8TNlTh9clP6vw3opMspqtLfUpy3LwXcwAGniyWNElrqLmT+nYt9SG8Xrp4XqW+E9/VOuLbdhvF1MLm71V75NWfEWdSvHw8bxT9wy6M9amJ2Jj9H+QB5Adv9cb6QEXDmjBOF6oPWYZT5bDDtc/FZ5G/lc=;
12, 24 -- Message-ID: {57259.59281.qm-at-web110801.mail.gq1.yahoo.com}
12, 24 -- X-YMail-OSG: QjN7QqwVM1lXkSAUNK8TehbKYwE_zElXLX7KY8wPgX5alR_WB9cJi7LC7M3cz14X0IHuSbEGHSFdLvi.ezEBgvmFHv4hHnWSXCnyqfEIDc5d2ADpz8T5TPA5DmogTTYxv3CN5DscAFkOOCEYxDgxJQk_8qLPrmWSP0UgyzCByl8XiGVniJEGcEKoSfWKbUEIBFdHzjxNciw0lyxd_pT0qDx69bZY3j59XBmpupvFDsV.cgmw9pI7Gf24yT..GdqpVdN5jgYI9LhJOpCwUDtYFzMSkXzbsPePyvrqRyDaaK14nS5fIISsQ_MCgtjJ9Q.CC4CpFPqLAEc7PGBOjx8ojl6E1e2At4OE4S555P3zrA--
12, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Thu, 14 May 2009 07:55:55 PDT
12, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
12, 24 -- References: {200905131337.n4DDb8xW007653-at-ns.microscopy.com}
12, 24 -- Date: Thu, 14 May 2009 07:55:55 -0700 (PDT)
12, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
12, 24 -- Subject: Re: [Microscopy] Re: viaWWW: optical staining of colorless living
12, 24 -- To: microscopy-at-microscopy.com
12, 24 -- In-Reply-To: {200905131337.n4DDb8xW007653-at-ns.microscopy.com}
12, 24 -- MIME-Version: 1.0
12, 24 -- Content-Type: text/plain; charset=iso-8859-1
12, 24 -- Content-Transfer-Encoding: 8bit
12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EEtwrW026724
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 14 May 2009 10:44:44 -0500
Subject: [Microscopy] Re: viaWWW: optical staining of colorless living

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Different objectives have different corrections for chromatic and other
aberrations. If a achromatic objective is used, green light has the
highest corrections, so red and blue will be a little fuzzy or off set in
the image. I suspect, images taken with pure blue light will have the same
uncorrected values. So you focus for blue, but that causes a difference in
your image. Repeat that with red and the same effects. Combine the images
and all the points will not fall on each other.

Use a apochromatic objective, well now you've got focus correction for
three wavelengths and spherical correction for two. Combining images
should work better, but not better, I suspect, that using white light.

Many microscopes used to come with a green filter (back in the B&W days)
because green light has the best correction in achromatic or apochromatic.
Substage condensors also played a role in image quality. Us codgers used
to wish upon a star, wanting apos instead of achro.

I suspect sharply defined z-planes were the effect of high NA objectives,
open substage iris and careful focus.

Stay safe..
Frank



nizets2-at-yahoo.com

05/14/2009 11:15 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
nizets2-at-yahoo.com [Microscopy] viaWWW: optical
staining of colorless living













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



Hi!

I seem to remember an interesting article in Microscopy today explaining
the advantage of illuminating a sample with one wavelength. One
could sequentially illuminate with 3 different wavelengthes and then merge
the 3 images to obtain a sharper final image.
In my brain of biologist the explanation had something to do with the fact
that a light containing different wavelengthes (like in white light)
interfere in different planes (although very near).
Sorry if my explanation seems confuse, it probably reflects my
understanding of the phenomena ;-)

Regards,

Stephane


} ----------------------------------------------------------------------------

} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------

} Remember this posting is most likely not from a Subscriber, so when
replying
} please  copy  both schaffne-at-patho.unibe.ch as well as  the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------

}
} Email:
} Name: Thomas Schaffner
}
} Organization: Institute of Pathology University of Bern
}
} Title-Subject: [Filtered] Differential optical staining of colorless
} living Organisms
}
} Question: Hi, I wonder about an elegant technique shown at our
} Institute about 35 years ago by R(obert) F Smith, then retired
} photographer at the Brookhaven National Laboratory. He published an
} article in J. Biol. Phot. Assoc Vol: 23, Nos 2 and 3, pages 74-77;
} 1955 entitled "Differential optical staining of colorless living
} organisms in macrophotography", unfortunately unavailable through our
} libraries.
} The brilliant colored images of live fish embryos in sharply defined
} z-planes were apparently obtained with polarized colored light
} sources generating some kind of interference contrast if I am not
} mistaken.
} I now wonder about the physical principles that were involved and how
} the stereoscope he usaed had to be set up.
} Any comments are welcome.
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original
Headers==============================
4, 24 -- From oshel1pe-at-cmich.edu Wed May 13 08:23:34 2009
4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 24 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n4DDNXFH000584
4, 24 --     for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 08:23:34
-0500
4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 24 --     by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id
n4DDNSWp027364
4, 24 --     for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 09:23:30
-0400
4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by
egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 24 --     Wed, 13 May 2009 09:23:27 -0400
4, 24 -- Mime-Version: 1.0
4, 24 -- Message-Id: {f06240808c6307a3a167c-at-[141.209.160.249]}
4, 24 -- Date: Wed, 13 May 2009 09:23:24 -0400
4, 24 -- To: Microscopy-at-microscopy.com
4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 24 -- Subject:  Re: [Microscopy] viaWWW: optical staining of colorless
living
4, 24 --  Organisms
4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 24 -- X-OriginalArrivalTime: 13 May 2009 13:23:27.0894 (UTC) FILETIME=
[00531B60:01C9D3CE]
4, 24 -- X-Canit-CHI2: 0.00
4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 24 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes
(0.0001,-0.5)
4, 24 -- X-CanItPRO-Stream: default
4, 24 -- X-Canit-Stats-ID: 13269997 - 07a5bab390cb
4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of -
Headers==============================






==============================Original
Headers==============================
12, 24 -- From nizets2-at-yahoo.com Thu May 14 09:56:01 2009
12, 24 -- Received: from web110801.mail.gq1.yahoo.com
(web110801.mail.gq1.yahoo.com [67.195.13.224])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n4EEtwrW026724
12, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009
09:55:59 -0500
12, 24 -- Received: (qmail 60072 invoked by uid 60001); 14 May 2009
14:55:56 -0000
12, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=yahoo.com; s=s1024; t=1242312956;
bh=3RJfD8Povpg0DJVJdklASkg/s9yTEUF8HfQbpUQVwsk=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=fozIxk6OdXBV6PUyiRNAj8+ch1NgIw3dWXUIzCbPAEGQixj33YjX55scMhwyEUZT9xvb1qv
+8
+Z8an4MmOcTEkAlTOigdLp91foqDi57u5GpFrOr63qw91P/F7G/j0rQ2vNEDy3g5xjZSIWOcKXvJgNfuv4NyiQ2LNp7rUrnRSc=

12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 24 -- s=s1024; d=yahoo.com;
12, 24 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;

12, 24 --
b=slhNGtyryROKrx/+Ehb8TNlTh9clP6vw3opMspqtLfUpy3LwXcwAGniyWNElrqLmT
+nYt9SG8Xrp4XqW+E9/VOuLbdhvF1MLm71V75NWfEWdSvHw8bxT9wy6M9amJ2Jj9H
+QB5Adv9cb6QEXDmjBOF6oPWYZT5bDDtc/FZ5G/lc=;
12, 24 -- Message-ID: {57259.59281.qm-at-web110801.mail.gq1.yahoo.com}
12, 24 -- X-YMail-OSG:
QjN7QqwVM1lXkSAUNK8TehbKYwE_zElXLX7KY8wPgX5alR_WB9cJi7LC7M3cz14X0IHuSbEGHSFdLvi.ezEBgvmFHv4hHnWSXCnyqfEIDc5d2ADpz8T5TPA5DmogTTYxv3CN5DscAFkOOCEYxDgxJQk_8qLPrmWSP0UgyzCByl8XiGVniJEGcEKoSfWKbUEIBFdHzjxNciw0lyxd_pT0qDx69bZY3j59XBmpupvFDsV.cgmw9pI7Gf24yT..GdqpVdN5jgYI9LhJOpCwUDtYFzMSkXzbsPePyvrqRyDaaK14nS5fIISsQ_MCgtjJ9Q.CC4CpFPqLAEc7PGBOjx8ojl6E1e2At4OE4S555P3zrA--

12, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com
via HTTP; Thu, 14 May 2009 07:55:55 PDT
12, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
12, 24 -- References: {200905131337.n4DDb8xW007653-at-ns.microscopy.com}
12, 24 -- Date: Thu, 14 May 2009 07:55:55 -0700 (PDT)
12, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
12, 24 -- Subject: Re: [Microscopy] Re: viaWWW: optical staining of
colorless living
12, 24 -- To: microscopy-at-microscopy.com
12, 24 -- In-Reply-To: {200905131337.n4DDb8xW007653-at-ns.microscopy.com}
12, 24 -- MIME-Version: 1.0
12, 24 -- Content-Type: text/plain; charset=iso-8859-1
12, 24 -- Content-Transfer-Encoding: 8bit
12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n4EEtwrW026724
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************



==============================Original Headers==============================
3, 24 -- From frank_karl-at-lincolnelectric.com Thu May 14 10:44:44 2009
3, 24 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EFihUi011059
3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:44:44 -0500
3, 24 -- In-Reply-To: {200905141515.n4EFFTI6007474-at-ns.microscopy.com}
3, 24 -- Subject: Re: [Microscopy] viaWWW: optical staining of colorless living
3, 24 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com
3, 24 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
3, 24 -- Message-ID: {OFA188FCE1.597E3BA8-ON852575B6.005465AF-852575B6.00567427-at-lincolnelectric.com}
3, 24 -- Date: Thu, 14 May 2009 11:44:19 -0400
3, 24 -- From: Frank_Karl-at-lincolnelectric.com
3, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM,
3, 24 -- CD-MIME complete at 05/14/2009 11:44:19 AM,
3, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM,
3, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM,
3, 24 -- Serialize complete at 05/14/2009 11:44:19 AM
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- charset="ISO-8859-1"
3, 24 -- Content-Transfer-Encoding: 8bit
3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EFihUi011059
==============================End of - Headers==============================




From: riba-at-umd.edu
Date: Thu, 14 May 2009 11:12:36 -0500
Subject: [Microscopy] FW: LM Need manuals for equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have several pieces of optical equipment that seem to be working but we
do not have the manuals for them.  If anyone has copies of the manuals for
the following equipment please contact me at riba-at-umd.edu .

THORN EMI GENCOM Inc. C-10 Photon counter
Acton Research Corporation monochromator Model No. 275
VELMEX 86 mm control/driver

Thanks a lot.

Lourdes Salamanca-Riba

Dr. L. Salamanca-Riba
Professor
Materials Science and Engineering Dept.
University of Maryland
College Park, MD 20742
(301) 405-5220
riba-at-umd.edu




==============================Original Headers==============================
9, 22 -- From riba-at-umd.edu Thu May 14 11:12:36 2009
9, 22 -- Received: from md4.mail.umd.edu (md4.mail.umd.edu [128.8.31.174])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EGCZKv026795
9, 22 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 11:12:35 -0500
9, 22 -- Received: from LSalamancaRPC (129-2-175-79.wireless.umd.edu [129.2.175.79])
9, 22 -- by md4.mail.umd.edu (MOS 3.10.4-GA)
9, 22 -- with ESMTP id AKW34985 (AUTH riba);
9, 22 -- Thu, 14 May 2009 12:12:34 -0400 (EDT)
9, 22 -- From: "Lourdes Salamanca-Riba" {riba-at-umd.edu}
9, 22 -- To: {microscopy-at-microscopy.com}
9, 22 -- Subject: FW: LM Need manuals for equipment
9, 22 -- Date: Thu, 14 May 2009 12:12:20 -0400
9, 22 -- Message-ID: {003201c9d4ae$c42ea8c0$4c8bfa40$-at-edu}
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain;
9, 22 -- charset="iso-8859-1"
9, 22 -- X-Mailer: Microsoft Office Outlook 12.0
9, 22 -- Thread-index: AcnUrV+AkOhabKa8TemZgXWldPxEoAAAUZuA
9, 22 -- Content-Language: en-us
9, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at md4.mail.umd.edu)
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EGCZKv026795
==============================End of - Headers==============================




From: Debra.Moreau-at-AGR.GC.CA
Date: Thu, 14 May 2009 11:33:51 -0500
Subject: [Microscopy] Re: Comments on Zeiss ID 03

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good afternoon.

I have just inherited a Zeiss Invertoscope ID 03 and have pulled the operating instructions off the web. Unfortunately, I was not able to find any comments from reviewers on the limitations or advantages of this little scope. Does anyone have any experience with this particular model?

Warmest Regards
Deb

Debra Moreau, Ph.D.
Food Safety & Quality | Salubrité at qualité des ailments
Agriculture and Agri-Food Canada | Agriculture et Agroalimentaire Canada
32 Main Street | 32 Rue Main
Kentville, Nova Scotia | Nouvelle Écosse B4N 1J5
Debra.Moreau-at-agr.gc.ca {mailto:Debbie.Moreau-at-agr.gc.ca}
Telephone | Téléphone 902-679-5710
Facsimile | Télécopieur 902-679-2311
Teletypewriter | Téléimprimeur 613-759-7470
Government of Canada | Gouvernement du Canada




==============================Original Headers==============================
7, 22 -- From Debra.Moreau-at-AGR.GC.CA Thu May 14 11:33:51 2009
7, 22 -- Received: from agrpazsmtp8.agr.gc.ca (agrpazsmtp8.agr.gc.ca [192.197.71.119])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EGXo8n009739
7, 22 -- for {Microscopy-at-Microscopy.com} ; Thu, 14 May 2009 11:33:51 -0500
7, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 22 -- Content-class: urn:content-classes:message
7, 22 -- MIME-Version: 1.0
7, 22 -- Content-Type: text/plain;
7, 22 -- charset="iso-8859-1"
7, 22 -- Subject: Re: Comments on Zeiss ID 03
7, 22 -- Date: Thu, 14 May 2009 12:33:49 -0400
7, 22 -- Message-ID: {00CAA86962A9F24E84F35531C7CE65DC0111F0F9-at-ONOTTAXMS4.AGR.GC.CA}
7, 22 -- X-MS-Has-Attach:
7, 22 -- X-MS-TNEF-Correlator:
7, 22 -- Thread-Topic: Re: Comments on Zeiss ID 03
7, 22 -- Thread-Index: AcnUscK9DAK13Ja3Qv26980gnMkbcQ==
7, 22 -- From: "Moreau, Debra" {Debra.Moreau-at-AGR.GC.CA}
7, 22 -- To: {Microscopy-at-Microscopy.com}
7, 22 -- X-OriginalArrivalTime: 14 May 2009 16:33:50.0840 (UTC) FILETIME=[C3581780:01C9D4B1]
7, 22 -- Received-SPF: none
7, 22 -- Content-Transfer-Encoding: 8bit
7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EGXo8n009739
==============================End of - Headers==============================




From: bfostermme-at-sbcglobal.net
Date: Thu, 14 May 2009 16:31:58 -0500
Subject: [Microscopy] viaWWW: optical staining of colorless

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Thomas,

I had a chance to teach on a course with Dr. Smith about that time. I agree with Phil that this is probably Rheinberg Illumination. The method that Phil has described will, indeed, give you one color on one side of a gradient (slope) and a different color on the opposite slope or gradient and that, under the right conditions, those images can appear more 3-dimensional. I also agree that the high NA objective is necessary for optical sectioning. And remember: this approach will be very directional: the gradients need to sit parallel to the filters for optimum effect.

Years ago, Kodak made a set of Rheinberg filters which we used in many many of our classes. When they stopped making them, we acquired the master and, at one time, had them available for a modest cost. Rather than the half-and-half model proposed by Phil, these sets are annular rings which can be used for magnifications up to about 16x. The underlying physics is pretty simple and is based on modifying the diffraction pattern used to form the image. To see a diffraction pattern, use a simple specimen (grating, the dots in the "fins" on pleurasigma angulatum, and, for those of you who do reflected light work, the regular parallel structures in a FinFET from a computer chip). If you are doing the experiment in transmitted light, remove the condenser and just place a pinhole over the light port. Remove the eyepiece and peer down the tube into the back focal plane of the objective (BFPo). Center the image of the pinhole in the middle of the BFPo. Put the sample on the stag!
e. (F
or reflected light, close the AI as tightly as possible). You should see a pattern of spots. The finer the structure in the sample, the more broadly spaced the spots in the pattern, so you may need play with different objectives in order to capture a sufficient amount of the pattern.

In any event: the central (Zero Order) spot in the diffraction pattern is responsible for the background. All the other diffracted spots are sample dependent and are responsible for carrying the "code" from the sample to the image for spacing, orientation, and edge information. If you color the Zero order, the background in the image will automatically be that color. If you color any of the diffracted orders, the specimen detail will automatically be that color. The simplest Rheinberg filters in the Kodak set have both central spots and rings, so you can make "sandwiches" to suit your sample and yourself. For example, we worked with a major Ketchup manufacture many years ago on an application for counting very thin filamentous mold strands. In normal brightfield, the strands were nearly invisible (soft gray against a white background). However, we provided our class with a set of Rheinbergs. If they used a clear central spot and, say, a turquoise outer ring, the mol!
d show
ed up a deep turquoise strands against a white background. Or, if they used a red central spot with a clear outer ring, the strands showed up as white against a red background. One combination we have found especially valuable is a blue central spot with a gold outer ring (OK: This is a test: what color was the background and what color was the mold?). For good, rich color, we advocate stacking 2 of each ring.

Similar filters can be made for your individual microscope by setting up Koehler illumination using a 10x or 16x objective, removing the eyepiece, and, while peering into the BFPo, opening the CONDENSER aperture so that the edges just touch the edge of the BFP, then VERY carefully, removing the condenser and measuring the diameter of the opening. That is the size necessary for the central spot. Next, measure the aperture of the condenser, fully open. The difference between the two is the width necessary for the outer ring. Just construct spots and rings from densely colored filter material (ex: available from Edmund Scientific). Stack the colors as you see fit and use a slim piece of tape over the edges to hold them together. To use, just set up Koehler illumination, open the condenser iris completely, and gently tuck the filter into roughly space just about where the iris is.

I've checked to see if we had any of the old sets available, but they appear to be packed away. Will try to unearth them and let you all know if /when they are available again. Rheinberg is an OLD technique (mid 1800's) but great for lots of routine observations and marvelous for teaching.

Hope this was helpful.

Best regards,
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310 Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now scheduling customized, on-site courses through September. Call me for a free assessment and quote.




: as with darkfield, there is a central region and a ring around it
At 08:43 AM 5/13/2009, oshel1pe-at-cmich.edu wrote:



--|----------------------------------------------------------------------------
--|The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
18, 21 -- From bfostermme-at-sbcglobal.net Thu May 14 16:31:57 2009
18, 21 -- Received: from smtp108.sbc.mail.mud.yahoo.com (smtp108.sbc.mail.mud.yahoo.com [68.142.198.207])
18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4ELVudB008611
18, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 16:31:57 -0500
18, 21 -- Message-Id: {200905142131.n4ELVudB008611-at-ns.microscopy.com}
18, 21 -- Received: (qmail 17632 invoked from network); 14 May 2009 21:31:56 -0000
18, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
18, 21 -- s=s1024; d=sbcglobal.net;
18, 21 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type;
18, 21 -- b=qdHsEP0HCTfeuN1qKtAbNs3tNG7NcMz6oKRn4CxMU/qAY3RZBsSXmCtq0EjMUh9PWO1sqNS68tF4ZOw6vTH0dxHXj+E9z3ayHdzWLRRMKRdsIyjRkK1QSXLdMB9UoYOBQdaHs6AGYPxi08/+H60HwsmaZHOzDE8FUs2Bzxvju5w= ;
18, 21 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login)
18, 21 -- by smtp108.sbc.mail.mud.yahoo.com with SMTP; 14 May 2009 21:31:56 -0000
18, 21 -- X-YMail-OSG: F4RacukVM1nVsm5_WNB1Ad9_NsXNUCI5i98fxbH3TF92nbLTn.87ihOcMM8qbtbY8x0tcQw8xP9g1GUlloCvAVUcWVHQkv5b.YF.o20szPeXRsh3B.8kO4F6shj466gw84IZHa4H2x5m5NPUnPD3694b2yAP0.8NYCsw1it2sv4GnBWC9mt.ssHWdDa_dWicKdobQcFgjbzA3sTyt07UThoVcOChi5zvFXPnwcIkaRpp1CBSTLLdrpiycSlkrqgOiYAUoiZqmDaKQdiUHp6H.y2svywsP9piLtqnxcrtXtW3w1HP1dp33.ljWRDzz90jqYuSa6fqgt.dzp4QHK6JqEeohzs-
18, 21 -- X-Yahoo-Newman-Property: ymail-3
18, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
18, 21 -- Date: Thu, 14 May 2009 16:25:56 -0500
18, 21 -- To: microscopy-at-microscopy.com
18, 21 -- From: Barbara Foster {bfostermme-at-sbcglobal.net}
18, 21 -- Subject: viaWWW: optical staining of colorless
18, 21 -- Mime-Version: 1.0
18, 21 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Sat, 16 May 2009 11:32:20 -0500
Subject: [Microscopy] CL detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group: does anyone have any idea who I can contact about repairs
for this model?
Thanks so much.
Barbara

==============================Original Headers==============================
1, 33 -- From maloneyb-at-fiu.edu Thu May 14 17:24:24 2009
1, 33 -- Received: from sodium.colo.stayonline.net (potassium.colo.stayonline.net [69.25.86.41])
1, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EMON1A025160
1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 17:24:24 -0500
1, 33 -- X-ASG-Debug-ID: 1242310383-3287001c0000-4CH8be
1, 33 -- X-Barracuda-URL: http://192.168.5.11:8000/cgi-bin/mark.cgi
1, 33 -- Received: from et-den-37.site.stayonline.net (unknown [12.69.19.194])
1, 33 -- by sodium.colo.stayonline.net (Spam Firewall) with ESMTP id ADA784557F7
1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:13:03 -0400 (EDT)
1, 33 -- Received: from et-den-37.site.stayonline.net ([12.69.19.194]) by sodium.colo.stayonline.net with ESMTP id JnKaL0xgmqpETH08 for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:13:03 -0400 (EDT)
1, 33 -- Received: from [192.168.50.99] ([192.168.50.99])
1, 33 -- by et-den-37.site.stayonline.net (8.13.8/8.12.6) with ESMTP id n4EED2T3057990
1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 14:13:03 GMT
1, 33 -- Message-ID: {4A0C26E6.4020105-at-fiu.edu}
1, 33 -- Disposition-Notification-To: Barbara {maloneyb-at-fiu.edu}
1, 33 -- Date: Thu, 14 May 2009 10:12:54 -0400
1, 33 -- From: Barbara {maloneyb-at-fiu.edu}
1, 33 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
1, 33 -- MIME-Version: 1.0
1, 33 -- To: microscopy-at-microscopy.com
1, 33 -- X-ASG-Orig-Subj: Reichert-Jung Model 250 microtome
1, 33 -- Subject: Reichert-Jung Model 250 microtome
1, 33 -- X-Priority: 2 (High)
1, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
1, 33 -- Content-Transfer-Encoding: 7bit
1, 33 -- X-Barracuda-Connect: UNKNOWN[12.69.19.194]
1, 33 -- X-Barracuda-Start-Time: 1242310384
1, 33 -- X-Barracuda-Spam-Score: 0.00
1, 33 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=
1, 33 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.1.25799
1, 33 -- Rule breakdown below
1, 33 -- pts rule name description
1, 33 -- ---- ---------------------- --------------------------------------------------
==============================End of - Headers==============================

From wa1hco-at-adelphia.net Thu May 14 21:57:16 2009
Return-Path: {wa1hco-at-adelphia.net}
Received: from google.com (198.subnet125-161-87.speedy.telkom.net.id [125.161.87.198] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4F2vElB016747
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 14 May 2009 21:57:15 -0500
Received: from [124.116.22.183] (HELO google.com)
by luckybulls.cn; Fri, 15 May 2009 09:57:12 +0700

Multi-year service agreement needed on our electron microscopes.

Bids will be opened and contract awarded June 3rd.

Bid package is located at the following URL:
http://www2.winthrop.edu/procurement/bids.htm

Microscopes are:
ISI super IIIA with EDS
Zeiss EM10C with top-entry gonio
Both have been continuously under contract since installation here


Please direct any questions initially through Teresia Sexton at:

Teresia C. Sexton
Procurement Officer
Winthrop University
Purchasing Services & Risk Management
307 Tillman Hall
Rock Hill, SC 29733
Telephone: 803-323-2143, ext. 6026
Fax: 803-323-2564
sextont-at-winthrop.edu


--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)


==============================Original Headers==============================
11, 20 -- From smithj-at-winthrop.edu Fri May 15 08:25:23 2009
11, 20 -- Received: from messenger.winthrop.edu (messenger.winthrop.edu [199.79.254.177])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4FDPK1t026307
11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 May 2009 08:25:21 -0500
11, 20 -- Received: from berlin.win.winthrop.edu (berlin.win.winthrop.edu [10.2.0.22])
11, 20 -- by messenger.winthrop.edu (MOS 3.8.6-GA)
11, 20 -- with ESMTP id GTA18783;
11, 20 -- Fri, 15 May 2009 09:25:16 -0400 (EDT)
11, 20 -- Received: from Julian-Smiths-Computer.local ([10.3.84.33]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959);
11, 20 -- Fri, 15 May 2009 09:25:15 -0400
11, 20 -- Message-ID: {4A0D6D3B.7020003-at-winthrop.edu}
11, 20 -- Date: Fri, 15 May 2009 09:25:15 -0400
11, 20 -- From: Julian Smith III {smithj-at-winthrop.edu}
11, 20 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
11, 20 -- MIME-Version: 1.0
11, 20 -- To: microscopy-at-microscopy.com
11, 20 -- Subject: Service contract for SEM and TEM needed in Southeastern US
11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 20 -- Content-Transfer-Encoding: 7bit
11, 20 -- X-OriginalArrivalTime: 15 May 2009 13:25:15.0685 (UTC) FILETIME=[95663550:01C9D560]
==============================End of - Headers==============================

From olkpvimpg_rhc-at-emapfrance.com Fri May 15 23:49:34 2009
Return-Path: {olkpvimpg_rhc-at-emapfrance.com}
Received: from google.com (p5210-ipad08morioka.iwate.ocn.ne.jp [60.37.120.210])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4G4nWUN014867
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 15 May 2009 23:49:33 -0500
Received: from [98.82.46.182] (HELO google.com)
by thinycleansneak.org; Sat, 16 May 2009 14:19:41 +0930

Hello all,

I need your critique on CL options available, overall, and at this specific moment I need info on using CL for Geological applications (zircons, etc).

I have some info from Gatan and have come across the CL option from KE Development as well.

Are there any others out there, how well does the one you are using work for EM and uProbe applications? (be sure to tell me your CL MFG and model, apps, and spectral range to allow me to insure that I know how to match up requirments now and in the future) Pros / Cons, etc.

You may contact me off list, I need to make the best decision possible for a customer and do not want to steer them wrong due to pricing or poor judgement.

Thank you for your time and consideration!!!


--
Joe Ullmer

JoeXray LLC
7958 Dubois Road
Carlisle, OHIO 45005
OFFICE / FAX: 937 550-9224
Cell: 937 554-2628
joexray-at-cinci.rr.com
www.joexray.net

==============================Original Headers==============================
9, 19 -- From joexray-at-cinci.rr.com Sat May 16 11:32:20 2009
9, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4GGWK6Q012091
9, 19 -- for {Microscopy-at-Microscopy.Com} ; Sat, 16 May 2009 11:32:20 -0500
9, 19 -- Received: from hrndva-web05-z02 ([10.128.132.156])
9, 19 -- by hrndva-smta02.mail.rr.com with ESMTP
9, 19 -- id {20090516163219689.LMGA18742-at-hrndva-smta02.mail.rr.com}
9, 19 -- for {Microscopy-at-Microscopy.Com} ; Sat, 16 May 2009 16:32:19 +0000
9, 19 -- Received: from 24.209.0.20 by webmail.cinci.rr.com; Sat, 16 May 2009 16:32:03 +0000
9, 19 -- Message-ID: {20090516163219.80PMO.459706.root-at-hrndva-web05-z02}
9, 19 -- Date: Sat, 16 May 2009 12:32:19 -0400
9, 19 -- From: {joexray-at-cinci.rr.com}
9, 19 -- To: Microscopy {Microscopy-at-Microscopy.Com}
9, 19 -- Subject: CL detectors
9, 19 -- MIME-Version: 1.0
9, 19 -- Content-Type: text/plain; charset=utf-8
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- X-Priority: 3 (Normal)
9, 19 -- Sensitivity: Normal
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 18 May 2009 07:29:58 -0500
Subject: [Microscopy] Wavy SEM images- resolved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to thank everyone who helped with suggestions and ideas about the
source of my wavy SEM images. I was very impressed the response from the
JEOL community who called and e-mailed me.

Thanks everyone!

The problem was traced to a defective pump on the water chiller. I
suspected the motor, but I was wrong. It was the pump.

Stay safe...........
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
7, 21 -- From frank_karl-at-lincolnelectric.com Mon May 18 07:29:57 2009
7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ICTvnW032237
7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 18 May 2009 07:29:57 -0500
7, 21 -- Subject: Wavy SEM images- resolved
7, 21 -- To: Microscopy-at-microscopy.com
7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 21 -- Message-ID: {OF0CE82389.F4641E40-ON852575BA.0043E32B-852575BA.00449F96-at-lincolnelectric.com}
7, 21 -- Date: Mon, 18 May 2009 08:29:45 -0400
7, 21 -- From: Frank_Karl-at-lincolnelectric.com
7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM,
7, 21 -- CD-MIME complete at 05/18/2009 08:29:47 AM,
7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM,
7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM,
7, 21 -- Serialize complete at 05/18/2009 08:29:47 AM
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: sergey-at-seas.ucla.edu
Date: Mon, 18 May 2009 13:39:40 -0500
Subject: [Microscopy] viaWWW: Old Cambridge Stereoscan 250 Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sergey-at-seas.ucla.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sergey-at-seas.ucla.edu
Name: Sergey Prikhodko

Organization: UCLA

Title-Subject: [Filtered] Old Cambridge Stereoscan 250

Question: Dear all,

We have old SEM Cambridge Stereioscan for sale. System is in
reasonably good condition though turbomolecular pump controller
required to be fixed. Sysytem has digital image aquisition with SE
(E-T) and BSE (solid state)detectors as well as EDS detector (thin
Be-window) attached for elemental analysis (Na and higher at. #),
line scan and x-ray mapping.

Login Host: 128.97.83.27
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon May 18 13:39:40 2009
7, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4IIdcA4031640
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 18 May 2009 13:39:39 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c6375acf6230-at-[206.69.208.22]}
7, 11 -- Date: Mon, 18 May 2009 13:39:36 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: sergey-at-seas.ucla.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Old Cambridge Stereoscan 250 Available
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: eric.roth-at-med.nyu.edu
Date: Tue, 19 May 2009 19:14:02 -0500
Subject: [Microscopy] viaWWW: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both eric.roth-at-med.nyu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: eric.roth-at-med.nyu.edu
Name: Eric W. Roth

Organization: NYU

Title-Subject: [Filtered] Myelin problem

Question: Hello list,

We are having a problem with getting very low contrast and
precipitation in the myelin of some cultured Neuron cells.

Here is the protocol that we are using for processing:
rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the
fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M
sucrose at room temperature for 30min, then 4C overnight. After rinse
3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in
0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.

The sample was sectioned at 100nm and imaged at 120kV.
Here is a link to download a couple good examples of our problem.

http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip

Any ideas would be greatly appreciated. Thanks!
Eric

Login Host: 216.165.126.103
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Tue May 19 19:14:01 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4K0E1xN031150
11, 11 -- for {microscopy-at-microscopy.com} ; Tue, 19 May 2009 19:14:01 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]}
11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Myelin problem
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 20 May 2009 03:15:19 -0500
Subject: [Microscopy] viaWWW: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Eric,

I would advise to make a search on the subject because it is not the first question about myelin embedding.
You could try "microscopy listserver myelin" in google.

I have no experience with myelin embedding but I can tell you that phosphate ions precipitate in presence of Ca2+ and most biological tissues contain Ca2+.
This may be the reason of the precipitates. You could try Hepes buffer or the very classical cacodylate. To increase the contrast you could try potassium ferrocyanate in osmium tetroxyde. I wrote about it some time about in microscopy today. Just search the listserver. It may well all be the contrasting solutions, I don't know how experienced you or your lab are with EM techniques. Lead citrate can precipitate pretty easily.

Best regards,

Stephane (didn't look at the pics)

 


----- Original Message ----
X-from: "eric.roth-at-med.nyu.edu" {eric.roth-at-med.nyu.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, May 20, 2009 2:19:47 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both eric.roth-at-med.nyu.edu as well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: eric.roth-at-med.nyu.edu
Name: Eric W. Roth

Organization: NYU

Title-Subject: [Filtered] Myelin problem

Question: Hello list,

We are having a problem with getting very low contrast and
precipitation in the myelin of some cultured Neuron cells.

Here is the protocol that we are using for processing:
rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the
fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M
sucrose at room temperature for 30min, then 4C overnight. After rinse
3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in
0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.

The sample was sectioned at 100nm and imaged at 120kV.
Here is a link to download a couple good examples of our problem.

http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip

Any ideas would be greatly appreciated.  Thanks!
Eric

  Login Host: 216.165.126.103
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Tue May 19 19:14:01 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4K0E1xN031150
11, 11 --     for {microscopy-at-microscopy.com} ; Tue, 19 May 2009 19:14:01 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]}
11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Myelin problem
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
29, 25 -- From nizets2-at-yahoo.com Wed May 20 03:15:19 2009
29, 25 -- Received: from web110810.mail.gq1.yahoo.com (web110810.mail.gq1.yahoo.com [67.195.13.233])
29, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4K8FJog029097
29, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 03:15:19 -0500
29, 25 -- Received: (qmail 13481 invoked by uid 60001); 20 May 2009 08:15:18 -0000
29, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1242807318; bh=5q3KQg4pvH+zdx0fG+ZMffubmOc2Z47nHm1rB/jGEX0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=pthdRm0v3qyqgsKJx3dvo1XOgk5hdKceFOyD0UYS9fMmGaLs366VERjqssX/46hGrfjgakc/JSSpwOA2YvyKFC8H7w8lwF4MKLu4SCntnHEeq/zFNMG5eVbkdKxPBCLmXe72u8yi+T46YEYoED/o0cgMOVS+8LF74GpnoJDeN+k=
29, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
29, 25 -- s=s1024; d=yahoo.com;
29, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
29, 25 -- b=oLNjQoMtzUrkNEUyHAlSeUCnIiOBT3bgHbRZivDbvYNcsAFSSxqrT7qynQI3kRXBLGOsfdVudssBlobfKMmrDvf0Kt1ETrZFs9Hm8OoesCCIC0chklj9+9AeWvK21Wr9OH2E50Vkp00XGCrZjB3JIz5IIKoZYj3xPA0jG6pO9wA=;
29, 25 -- Message-ID: {575886.13457.qm-at-web110810.mail.gq1.yahoo.com}
29, 25 -- X-YMail-OSG: B_2PfJYVM1mSCJaAT1X_v2pCYOeyG6Bf7c2kd6PTGrar2gjXeKq4YJ04lo4dcb.ASeS_FIZFBJaEsnnMXWRC6s3JoW_EBdY6NdG2u3iqKS1ePP5YnmstW2kUngsqSvbvssyrcXJibQUB56QIvioCQzEd6DP9DG9SGwnuJWyVdTF4N_dRy3M5yu83xuZKHB3YZIyIahActLFZMXPq.FVaZikWqukIJt3OBKVo0_45Q6n_Wx1NdxNgO1KNWVzXE9xm1Ym1P.lIXarzQO91GXPm7sh1gUUPs5KUd0gP7DcFhcZIaUP3B6yugPZRHDNe7.TrUlXcI.miF7MX3L6Yk82l9wlg
29, 25 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Wed, 20 May 2009 01:15:18 PDT
29, 25 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
29, 25 -- References: {200905200019.n4K0Jlps005246-at-ns.microscopy.com}
29, 25 -- Date: Wed, 20 May 2009 01:15:18 -0700 (PDT)
29, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
29, 25 -- Subject: Re: [Microscopy] viaWWW: Myelin problem
29, 25 -- To: eric.roth-at-med.nyu.edu
29, 25 -- Cc: microscopy-at-microscopy.com
29, 25 -- In-Reply-To: {200905200019.n4K0Jlps005246-at-ns.microscopy.com}
29, 25 -- MIME-Version: 1.0
29, 25 -- Content-Type: text/plain; charset=iso-8859-1
29, 25 -- Content-Transfer-Encoding: 8bit
29, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4K8FJog029097
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Wed, 20 May 2009 05:40:49 -0500
Subject: [Microscopy] Re: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


(unfortunately, my original mailing from the MSA-spam-filter was
rejected for the following reason(s): Content-Type: multipart/alternative

This was due to unintendingly having changed the original text-format to Rich format. I apologize for any inconveniencies this might have induced)
----------------------------------------------------------------------

Good morning, hello } Big Apple {,
dear Eric,

not to post a nasty/unpleasant comment about your problem.....
Have seen your nonetheless nice pic's and have seen that they are completely free of any precipitates, so the problem IMhO for the most part ( 90-99% ) doesn't cohere with your staining solutions.

I bet that you can improve the ultrastructural preservation of myelin sheaths if you shorten your initial fixiation to 15 - 30 [at the most 60!] min's at room temperature or say 15-30 mins at RT, + additional 30 mins -at- 4 degr.C.. From my experience I think that your fixation /fixative over night (despite at 4 degr.C) is "elutive" to/with your delicate cultured neuron cells (and if you check the images you can find some minor preserved membranes [==} organelles], as this perhaps also is true for the speckled appearance of the myelin lamellae). Maybe also a consequence of too rapid dehydration (or ddH2O after osmication). Unfortunately you did not comment on the duration and steps of your dehydration schedule.

Your using PO4 buffer within / for your fixative does not mean necessarily (IMhO) that you "precipitate away" all your Ca++ in your cells, otherwise nobody would use such a fixative any longer for research or diagnostic issues (but I confess that perhaps sodium-cacodylate or HEPES, as suggested by Stepane, would be a promising - also more expensive - alternative.... But: in my opinion you have to think also about the "elutivity" of buffers and fixatives in general, whereby } elutivity { is meant on a molecular scale for more/less hydrophilic substrata for the fixative used and consequences arising afterwards in the dehydration steps).

The preservation of myelin sheaths in most of the diagnostic probes in our lab, as I remember, has always been sufficient, nothing bad is to be said about use of PO4 as the (fix,washing, postfix-) buffer system.

Ca++ and its (biological) complexes (as most PO4-ions) will be precipitated in the higher alcohols (so if you start with e.g. buffer washes[ PO4] after GA-fixation only or additional postfixation by OsO4, and change into / start dehydration with EtOH 50%, then 70% 80,90,6,100% etc. ) you rarely will get Ca++ and or PO4-precipitates (cf. "salt & pepper") in your specimens.

A good choice for the preservation of myelin sheaths (because it's relatively easy to include in the standard processing) would be - after osmication as usual - then washing twice in buffer, then EtOH 50% (for cultured cells) max 2-5 min - then incubation in 1% para-phenylenediamine (PPD) in 70%EtOH (e.g. ca. 15-20 min -at-RT), washing once or twice in pure EtOH 70% and proceed with your standard dehydration steps .... this will retain lipids and glycoconjugates within the spec's (if you would like to read/hear more about that, please reply for the details, information and lit.ref. ).


Best wishes and good luck,

Wolfgang MUSS
EM-Lab, Pathology
SALK-PMU (Fed. & City Gen. Hosp.) SALZBURG
AUSTRIA-EUROPE

This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.


-----Ursprüngliche Nachricht-----
Von: eric.roth-at-med.nyu.edu [mailto:eric.roth-at-med.nyu.edu]
Gesendet: Mittwoch, 20. Mai 2009 02:19
An: Muß Wolfgang
Betreff: [Microscopy] Myelin problem

---------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both eric.roth-at-med.nyu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------
Email: eric.roth-at-med.nyu.edu
Name: Eric W. Roth
Organization: NYU
Title-Subject: [Filtered] Myelin problem
Question:

Hello list,
We are having a problem with getting very low contrast and precipitation in the myelin of some cultured Neuron cells.
Here is the protocol that we are using for processing:

rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M sucrose at room temperature for 30min, then 4C overnight. After rinse 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.

The sample was sectioned at 100nm and imaged at 120kV.
Here is a link to download a couple good examples of our problem.

http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip

Any ideas would be greatly appreciated. Thanks!
Eric

Login Host: 216.165.126.103
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Tue May 19 19:14:01 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4K0E1xN031150
11, 11 -- for {microscopy-at-microscopy.com} ; Tue, 19 May 2009 19:14:01 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]}
11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Myelin problem
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
25, 35 -- From W.Muss-at-salk.at Wed May 20 05:40:48 2009
25, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
25, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KAelqI017518
25, 35 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 05:40:48 -0500
25, 35 -- Received: from localhost (localhost [127.0.0.1])
25, 35 -- by hermes.salk.at (Postfix) with ESMTP id F1FA5C38E0;
25, 35 -- Wed, 20 May 2009 12:40:46 +0200 (CEST)
25, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
25, 35 -- Received: from hermes.salk.at ([127.0.0.1])
25, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
25, 35 -- with ESMTP id jvJKggW6CQoB; Wed, 20 May 2009 12:40:46 +0200 (CEST)
25, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
25, 35 -- by hermes.salk.at (Postfix) with ESMTP id 87F16C38D9;
25, 35 -- Wed, 20 May 2009 12:40:46 +0200 (CEST)
25, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
25, 35 -- Wed, 20 May 2009 12:40:46 +0200
25, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
25, 35 -- Content-class: urn:content-classes:message
25, 35 -- MIME-Version: 1.0
25, 35 -- Content-Type: text/plain;
25, 35 -- charset="iso-8859-1"
25, 35 -- Subject: [Microscopy] Re: Myelin problem
25, 35 -- Date: Wed, 20 May 2009 12:40:46 +0200
25, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0A65-at-N1RZ116.lksdom21.lks.local}
25, 35 -- X-MS-Has-Attach:
25, 35 -- X-MS-TNEF-Correlator:
25, 35 -- Thread-Topic: [Microscopy] Re: Myelin problem
25, 35 -- Thread-Index: AcnZN26oe8UTUUfnQy2/tZ0Mun8VwQ==
25, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
25, 35 -- To: {microscopy-at-microscopy.com}
25, 35 -- Cc: {eric.roth-at-med.nyu.edu}
25, 35 -- X-OriginalArrivalTime: 20 May 2009 10:40:46.0493 (UTC) FILETIME=[6EF7C4D0:01C9D937]
25, 35 -- X-Scanned-By: SALK-Content-Filter
25, 35 -- Content-Transfer-Encoding: 8bit
25, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4KAelqI017518
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Wed, 20 May 2009 07:53:36 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John:

Are you seriously stirring this up again? Didn't we just handle O2
displacement via nitrogen boil off a few weeks ago?

The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only
300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at
100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a
tiny room for a scope and all the N2 would have to be released
rapidly enough to displace the O2 in the room (or reduce it from 22%
to below 12% for this to become an issue let alone life threatening)
without the N2 leaking out the doorway or any O2 leaking in. But in
fact it sounds like ". . .going through nitrogen gas like there is no
tomorrow." is probably days not minutes, and the scope room is a
"normal room" and not a sealed environmental chamber.

I have worked with compressed atmospheric air. No matter how dry it
is an extremely corrosive material, degrading fittings, seals and
regulator parts. I would suggest the long term costs and safety make
working with compressed air a far worse choice than ppN2


On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Elgin;
} My I suggest also that you have created a significant safety hazard by
} running your air table, and I presume your valving, on nitrogen. The
} leak you now have (and everything develops leaks eventually) is
} filling up your room with pure nitrogen and displacing the oxygen. You
} should be using only clean dry air for these items so that when it
} leaks, the room fills up with.....air! Air that you can breath!
} Nitrogen should only be used for venting the chamber.
}
}
} Sent from my iPhone.
} John Mardinly
}
} On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
}
} }
} }
} }
} } ---
} } ---
} } --------------------------------------------------------------------
} } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help


Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


==============================Original Headers==============================
10, 25 -- From edelmare-at-muohio.edu Wed May 20 07:53:35 2009
10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.67])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KCrZNA004846
10, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 20 May 2009 07:53:35 -0500
10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrsGt014924;
10, 25 -- Wed, 20 May 2009 08:53:54 -0400
10, 25 -- Received: from [192.168.1.23] ([134.53.14.105])
10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrYF2021966;
10, 25 -- Wed, 20 May 2009 08:53:34 -0400
10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
10, 25 -- To: microscopy-at-Microscopy.com
10, 25 -- Date: Wed, 20 May 2009 08:53:34 -0400
10, 25 -- MIME-Version: 1.0
10, 25 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak
10, 25 -- CC: "jmardinly-at-gmail.com" {jmardinly-at-gmail.com}
10, 25 -- Message-ID: {4A13C50E.27113.A79CE8A-at-edelmare.muohio.edu}
10, 25 -- Priority: normal
10, 25 -- In-reply-to: {200905082112.n48LCJEb013069-at-ns.microscopy.com}
10, 25 -- References: {200905082112.n48LCJEb013069-at-ns.microscopy.com}
10, 25 -- X-mailer: Pegasus Mail for Windows (4.41)
10, 25 -- Content-type: text/plain; charset=US-ASCII
10, 25 -- Content-transfer-encoding: 7BIT
10, 25 -- Content-description: Mail message body
10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67
==============================End of - Headers==============================




From: henrik.kaker-at-guest.arnes.si
Date: Wed, 20 May 2009 08:11:20 -0500
Subject: [Microscopy] viaWWW: Monitor for JEOL JSM 35CF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both henrik.kaker-at-guest.arnes.si as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: henrik.kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: Metal Ravne, Slovenia

Title-Subject: [Filtered] Monitor for JEOL JSM 35CF

Question: Dear All,

We are looking for JEOL JSM 35CF monitor(not 35C type). We have a
truble with the current monitor and service can't repair the error.

Henrik Kaker
Metal Ravne
SEM-EDS Laboratory

Login Host: 89.212.22.21
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed May 20 08:11:19 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KDBC7H017585
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 08:11:16 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c639b1dccbc1-at-[206.69.208.22]}
8, 11 -- Date: Wed, 20 May 2009 08:11:12 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: henrik.kaker-at-guest.arnes.si (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Monitor for JEOL JSM 35CF
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gill.dealtry-at-nmmu.ac.za
Date: Wed, 20 May 2009 08:11:49 -0500
Subject: [Microscopy] viaWWW: frozen samples for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gill.dealtry-at-nmmu.ac.za as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gill.dealtry-at-nmmu.ac.za
Name: Dr Gill Dealtry

Organization: Nelson Mandela Metropolitan University

Title-Subject: [Filtered] frozen samples for LM

Question: I have access to unfixed tissue pieces that were dissected
from sacrificed rats, snap frozen within cryotubes in liquid nitrogen
and stored at -80 for biochemical analysis. Is it possible to use
this for cryo-sections for LM? How would I retrieve the tissue and
process it for cryostat sections?

Login Host: 192.96.15.22
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed May 20 08:11:49 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KDBlQZ018316
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 08:11:48 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c639b1f9d28f-at-[206.69.208.22]}
6, 11 -- Date: Wed, 20 May 2009 08:11:46 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: gill.dealtry-at-nmmu.ac.za (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: frozen samples for LM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 20 May 2009 08:33:34 -0500
Subject: [Microscopy] viaWWW: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Eric,

This looks amazingly similar to a problem we had several years ago that
resisted any and all attempts to fix it until we began using
2-mercaptoethanol in our protocols. Go to
http://www.emc.missouri.edu/pandp.htm to see our procedures. After
nearly two years of trying everything else we could think of, this is
the only thing that fixed the problem, and the problem has stayed fixed
since. Unless, that is, we try processing without 2-Me again.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: eric.roth-at-med.nyu.edu [mailto:eric.roth-at-med.nyu.edu]
Sent: Tuesday, May 19, 2009 7:15 PM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both eric.roth-at-med.nyu.edu as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: eric.roth-at-med.nyu.edu
Name: Eric W. Roth

Organization: NYU

Title-Subject: [Filtered] Myelin problem

Question: Hello list,

We are having a problem with getting very low contrast and
precipitation in the myelin of some cultured Neuron cells.

Here is the protocol that we are using for processing:
rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the
fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M
sucrose at room temperature for 30min, then 4C overnight. After rinse
3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in
0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.

The sample was sectioned at 100nm and imaged at 120kV.
Here is a link to download a couple good examples of our problem.

http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip

Any ideas would be greatly appreciated. Thanks!
Eric

Login Host: 216.165.126.103
------------------------------------------------------------------------
---

==============================Original
Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Tue May 19 19:14:01 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n4K0E1xN031150
11, 11 -- for {microscopy-at-microscopy.com} ; Tue, 19 May 2009
19:14:01 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]}
11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Myelin problem
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
23, 30 -- From TindallR-at-missouri.edu Wed May 20 08:33:34 2009
23, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KDXXsY016434
23, 30 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 08:33:33 -0500
23, 30 -- X-IronPort-Anti-Spam-Filtered: true
23, 30 -- X-IronPort-Anti-Spam-Result: ApoEAOOjE0rRauUp/2dsb2JhbAC+JgEBAQeHfohOglMBAYEzBYgu
23, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
23, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 May 2009 08:33:33 -0500
23, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
23, 30 -- Wed, 20 May 2009 08:33:33 -0500
23, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
23, 30 -- Content-class: urn:content-classes:message
23, 30 -- MIME-Version: 1.0
23, 30 -- Content-Type: text/plain;
23, 30 -- charset="us-ascii"
23, 30 -- Subject: RE: [Microscopy] viaWWW: Myelin problem
23, 30 -- Date: Wed, 20 May 2009 08:33:32 -0500
23, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD80F1-at-UM-XMAIL08.um.umsystem.edu}
23, 30 -- In-Reply-To: {200905200015.n4K0FRpH032738-at-ns.microscopy.com}
23, 30 -- X-MS-Has-Attach:
23, 30 -- X-MS-TNEF-Correlator:
23, 30 -- Thread-Topic: [Microscopy] viaWWW: Myelin problem
23, 30 -- Thread-Index: AcnY4BSn68RWBOqYR8Wb7K558MF7fQAbvfig
23, 30 -- References: {200905200015.n4K0FRpH032738-at-ns.microscopy.com}
23, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 30 -- To: {eric.roth-at-med.nyu.edu}
23, 30 -- Cc: {microscopy-at-microscopy.com}
23, 30 -- X-OriginalArrivalTime: 20 May 2009 13:33:33.0132 (UTC) FILETIME=[91F758C0:01C9D94F]
23, 30 -- Content-Transfer-Encoding: 8bit
23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4KDXXsY016434
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Wed, 20 May 2009 09:51:43 -0500
Subject: [Microscopy] Re: viaWWW: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Eric,
Regarding your precipitate problem, I'm not sure what the cause is
though pretty certain it's osmium-related as I've had similar problems
recently (not neuronal cells though) and it doesn't happen in non-osmicated
samples and it is not buffer-dependent. It certainly does not look like a
lead citrate problem as this, as you may well know, has the appearance of
quite large spherical dense spots all over the sections (caused if you
breathe on the solution whilst staining, thus creating Pb carbonate I
believe).
Anyway, I think I can help you get rid of it though, which may be good
news for you. Prepare a saturated aqueous solution of sodium metaperiodate
and place it in an ultrasonic bath for a while (15-30min) so that when you
shake it up a little it has a milky appearance. Pipette from this milky
region (avoiding the undissolved granules at the bottom) and place 20ul
drops on parafilm. Slide your grids in very gently from the side of the
drops to fully immerse (float them section side down if they're collected
on support grids though....and for the subsequent rinses). Leave for 30min
and then rinse by immersion in distilled water for 4 X 3min minimum or so.
When you do this, again slide the grids in and out of the water very gently
and at right angles to the water surface, otherwise you could lose your
sections. after this you can stain as normal, perhaps taking on board the
advice of some others on here about contrast improvement.
This normally works for me and hope it does for you!
Cheers,
Jules

Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

--On 19 May 2009 19:24 -0500 eric.roth-at-med.nyu.edu wrote:

}
}
}
} -------------------------------------------------------------------------
} --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------
} ---
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} -------------------------------------------------------------------------
} -- Remember this posting is most likely not from a Subscriber, so when
} replying please copy both eric.roth-at-med.nyu.edu as well as the
} MIcroscopy Listserver
} -------------------------------------------------------------------------
} --
}
} Email: eric.roth-at-med.nyu.edu
} Name: Eric W. Roth
}
} Organization: NYU
}
} Title-Subject: [Filtered] Myelin problem
}
} Question: Hello list,
}
} We are having a problem with getting very low contrast and
} precipitation in the myelin of some cultured Neuron cells.
}
} Here is the protocol that we are using for processing:
} rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the
} fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M
} sucrose at room temperature for 30min, then 4C overnight. After rinse
} 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in
} 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.
}
} The sample was sectioned at 100nm and imaged at 120kV.
} Here is a link to download a couple good examples of our problem.
}
} http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip
}
} Any ideas would be greatly appreciated. Thanks!
} Eric
}
} Login Host: 216.165.126.103
} -------------------------------------------------------------------------
} --
} ==============================Original
} Headers============================== 11, 11 -- From
} zaluzec-at-microscopy.com Tue May 19 19:14:01 2009
} 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22]) 11, 11 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n4K0E1xN031150 11, 11 -- for
} {microscopy-at-microscopy.com} ; Tue, 19 May 2009 19:14:01 -0500 11, 11 --
} Mime-Version: 1.0
} 11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]}
} 11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500
} 11, 11 -- To: microscopy-at-microscopy.com
} 11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver)
} 11, 11 -- Subject: viaWWW: Myelin problem
} 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================





==============================Original Headers==============================
9, 23 -- From bafg3-at-sussex.ac.uk Wed May 20 09:51:43 2009
9, 23 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KEpg0U001612
9, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 09:51:42 -0500
9, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:3827)
9, 23 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
9, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} )
9, 23 -- id KJY6M4-000DA9-KB; Wed, 20 May 2009 15:51:40 +0100
9, 23 -- Date: Wed, 20 May 2009 15:51:39 +0100
9, 23 -- To: eric.roth-at-med.nyu.edu, Microscopy-at-microscopy.com
9, 23 -- Subject: Re: [Microscopy] viaWWW: Myelin problem
9, 23 -- Message-ID: {1589150928.1242834699-at-ls0130.lifesci.susx.ac.uk}
9, 23 -- In-Reply-To: {200905200024.n4K0O9iJ011788-at-ns.microscopy.com}
9, 23 -- Originator-Info: login-token=Mulberry:01AM9vm2Iw1QI0D8/1aTMuQuQOdDTawmEqLkTa;
9, 23 -- token_authority=postmaster-at-central.susx.ac.uk
9, 23 -- X-Mailer: Mulberry/2.0.8 (Win32)
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed
9, 23 -- Content-Transfer-Encoding: 7bit
9, 23 -- Content-Disposition: inline
9, 23 -- X-Sussex: true
9, 23 -- X-Sussex-transport: remote_smtp_rew
9, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 20 May 2009 09:58:14 -0500
Subject: [Microscopy] Re: myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I neglected to point out in my previous post that our precipitate was
osmium (Dr. John Bozzola's lab helped us out with EDS and confirmed
this). The 2-Me works by reducing the osmium. We never found out why
the problem suddenly started happening, which is frustrating, but it
certainly cured it.

Also, the problem happened with many types of tissue.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
7, 27 -- From TindallR-at-missouri.edu Wed May 20 09:58:14 2009
7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KEwCrl007422
7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 09:58:13 -0500
7, 27 -- X-IronPort-Anti-Spam-Filtered: true
7, 27 -- X-IronPort-Anti-Spam-Result: ApoEANq3E0rRauUp/2dsb2JhbAC+YAEJiACIToJTgTUF
7, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 May 2009 09:58:00 -0500
7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 27 -- Wed, 20 May 2009 09:58:00 -0500
7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 27 -- Content-class: urn:content-classes:message
7, 27 -- MIME-Version: 1.0
7, 27 -- Content-Type: text/plain;
7, 27 -- charset="us-ascii"
7, 27 -- Subject: Re: myelin problem
7, 27 -- Date: Wed, 20 May 2009 09:58:00 -0500
7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD80F4-at-UM-XMAIL08.um.umsystem.edu}
7, 27 -- X-MS-Has-Attach:
7, 27 -- X-MS-TNEF-Correlator:
7, 27 -- Thread-Topic: Re: myelin problem
7, 27 -- Thread-Index: AcnZW14mXa03oh+GRkqYHT8zEAHqZw==
7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
7, 27 -- To: {microscopy-at-microscopy.com}
7, 27 -- X-OriginalArrivalTime: 20 May 2009 14:58:00.0657 (UTC) FILETIME=[5E724C10:01C9D95B]
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4KEwCrl007422
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Wed, 20 May 2009 09:58:57 -0500
Subject: [Microscopy] viaWWW: Myelin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Eric,
Regarding your precipitate problem, I'm not sure what the cause is
though pretty certain it's osmium-related as I've had similar problems
recently (not neuronal cells though) and it doesn't happen in non-osmicated
samples and it is not buffer-dependent. It certainly does not look like a
lead citrate problem as this, as you may well know, has the appearance of
quite large spherical dense spots all over the sections (caused if you
breathe on the solution whilst staining, thus creating Pb carbonate I
believe).
Anyway, I think I can help you get rid of it though, which may be good
news for you. Prepare a saturated aqueous solution of sodium metaperiodate
and place it in an ultrasonic bath for a while (15-30min) so that when you
shake it up a little it has a milky appearance. Pipette from this milky
region (avoiding the undissolved granules at the bottom) and place 20ul
drops on parafilm. Slide your grids in very gently from the side of the
drops to fully immerse (float them section side down if they're collected
on support grids though....and for the subsequent rinses). Leave for 30min
and then rinse by immersion in distilled water for 4 X 3min minimum or so.
When you do this, again slide the grids in and out of the water very gently
and at right angles to the water surface, otherwise you could lose your
sections. after this you can stain as normal, perhaps taking on board the
advice of some others on here about contrast improvement.
This normally works for me and hope it does for you!
Cheers,
Jules


Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

==============================Original Headers==============================
4, 23 -- From bafg3-at-sussex.ac.uk Wed May 20 09:58:57 2009
4, 23 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KEwtx3008548
4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 09:58:56 -0500
4, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:3879)
4, 23 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
4, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} )
4, 23 -- id KJY6Y7-000GJK-4D
4, 23 -- for Microscopy-at-microscopy.com; Wed, 20 May 2009 15:58:55 +0100
4, 23 -- Date: Wed, 20 May 2009 15:58:54 +0100
4, 23 -- To: Microscopy-at-microscopy.com
4, 23 -- Subject: [Microscopy] viaWWW: Myelin problem
4, 23 -- Message-ID: {1589585553.1242835134-at-ls0130.lifesci.susx.ac.uk}
4, 23 -- Originator-Info: login-token=Mulberry:01TYlm36/qzEaBycY5tu17/NtSwe7RBq7ke/7R;
4, 23 -- token_authority=postmaster-at-central.susx.ac.uk
4, 23 -- X-Mailer: Mulberry/2.0.8 (Win32)
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- Content-Disposition: inline
4, 23 -- X-Sussex: true
4, 23 -- X-Sussex-transport: remote_smtp_rew
4, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Wed, 20 May 2009 10:08:13 -0500
Subject: [Microscopy] MM2009 Search Engine now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

The MM2009 Program search engine is now on-line.

You can search the 2009 program using keywords in
the following fields

Symposium, Author& Affiliation, Paper Title

Go to http://www.microscopy.org
Select the link to the Annual Meeting (12th left hand button)
Select the link to the Search Engine (2nd left hand button)


Cheers,

Nestor
Your Friendly Neighborhood SysOp


==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed May 20 10:08:13 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KF8CQg006962
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 10:08:12 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c637a29e370d-at-[206.69.208.22]}
10, 11 -- Date: Wed, 20 May 2009 10:08:11 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
10, 11 -- Subject: MM2009 Search Engine now On-Line
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 20 May 2009 10:56:31 -0500
Subject: [Microscopy] Carbon fiber for Baltec evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

We use an Balzers/Baltec MED 010 to carbon coat our samples for TEM and
SEM. It uses carbon fiber thread for one shot flash evaporation (Baltec
Ref : LZ 02307 VN, 25 m length).

The french supplier we had for this carbon thread (which comes from
Baltec Lichetensten) has no stock, and needs allways 4 to more than 6
weeks for the delivery. So I've two sets of questions, one for users and
one for suppliers.

-For users : First what kind of other brand fiber can be used on
this device. And secondly, I suppose it should exist a way to treat
general purpose carbon fiber , which is generally insolating, to get it
conducting, and have a cheaper way than the expensive genuine part (up
to } 200. euro for 25 meter !).

-For supplier : the same question about what other brand of fiber
could be used. That supplied by Baltec is very thin, less then 1 mm in
diameter. I had once some from a well known europeen TEM/SEM accessories
supplier which was a real rope, nice for EPMA at high current, but much
too thick for the power supply, and giving too to thick films for TEM or
SEM imaging.
-For europeen supplier : Who would have the Baltec referenced fiber
on stock ? And at which price !!! (off list ?!)

Thanks to all for help and answers.

Jacques

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed May 20 10:56:30 2009
9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152])
9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KFuUMR028856
9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 10:56:30 -0500
9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n4KFuTSl012050
9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 17:56:29 +0200 (CEST)
9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 29 -- (No client certificate requested)
9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 916511000216
9, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 20 May 2009 17:51:15 +0200 (CEST)
9, 29 -- Message-ID: {4A142824.8010802-at-ipcms.u-strasbg.fr}
9, 29 -- Date: Wed, 20 May 2009 17:56:20 +0200
9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 29 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
9, 29 -- MIME-Version: 1.0
9, 29 -- To: Microscopy-at-microscopy.com
9, 29 -- Subject: Carbon fiber for Baltec evaporator
9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 29 -- Content-Transfer-Encoding: 8bit
9, 29 -- X-IPCMS-MailScanner: Found to be clean
9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 20 May 2009 17:56:29 +0200 (CEST)
9, 29 -- X-Virus-Scanned: ClamAV 0.94.2/9376/Wed May 20 16:21:28 2009 on mr2.u-strasbg.fr
9, 29 -- X-Virus-Status: Clean
9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
9, 29 -- autolearn=disabled version=3.2.5
9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr2.u-strasbg.fr
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Wed, 20 May 2009 12:57:41 -0500
Subject: [Microscopy] Re: Carbon fiber for Baltec evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jaques,

There is nothing magical about carbon fiber to make it conductive. The
conductivity will depend to some extent on how the elementary fibers are
formed and the overall cross-sectional area, roughly proportional to the
diameter, but variable due to the type of assembly (rope laid, braided,
etc.) As far as I can tell, the variety of carbon strings and
suitability for particular instruments is related to the electronics
that are used to heat them, and it seems that most systems have
particular set voltages and power capacities and need a particular fiber
characteristic to match the equipment. For example, if a braid that is
larger dia and lower resistance is used in place of the smaller dia
braid that a power supply is designed to use, the power supply may put
out all of its current, maybe even too much, with associated damage, and
not have enough power to heat the braid to the sublimation temperature.

The size of the braid, power supply characteristics and physical
geometry of the evaporation source to sample are set by the manufacturer
to give the predictable result. The manufacturer's quality control is
certainly set to maintain this predictable result with their equipment.

Having said this as if there is no choice in the matter, I can say that
we use carbon braid in systems designed for carbon rods and since these
older systems typically have a 1kW power transformer and a variable
autotransformer to drive the power transformer, it is possible to tune
the voltage of these systems for many types of carbon "string" (string,
yarn, braid, cord....) and also choose the way in which it sublimes;
multiple pulses to white hot until it no longer conducts rather than
"flash" evaporation is my choice. In an old Kinney Evaporator I use the
tungsten basket/boat holders with a 1.25cm insulating spacer and clamp a
short piece of carbon string (SPI #11433) between. Using ~30% setting of
the autotransformer (gives 30% of the 24VAC secondary to power the
heating) I evaporate a single piece of carbon at ~5cm onto mica to make
pure carbon films.

I have collected a bit of data, have SEM pictures of the "burned out"
string tips (to show they don't explode but sublime away to an open
circuit) and some Voltage-current data for my setup. Since I can't make
attachments to the list, I will make the offer to share this info and if
anyone is further interested I would be happy to send or post on a website.

There is a notable paper on this topic although it is unfortunate that
very little information was given on the power supply details even
though this was otherwise a very technical report:

Precise and reproducible deposition of thin and ultra-thin carbon films
by flash evaporation of carbon yarn in high vacuum. Klaus-Ruediger
Peters. J. Micros. (133), p 17. 1984.

Dale Callaham

jacques.faerber-at-ipcms.u-strasbg.fr wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi all
}
} We use an Balzers/Baltec MED 010 to carbon coat our samples for TEM and
} SEM. It uses carbon fiber thread for one shot flash evaporation (Baltec
} Ref : LZ 02307 VN, 25 m length).
}
} The french supplier we had for this carbon thread (which comes from
} Baltec Lichetensten) has no stock, and needs allways 4 to more than 6
} weeks for the delivery. So I've two sets of questions, one for users and
} one for suppliers.
}
} -For users : First what kind of other brand fiber can be used on
} this device. And secondly, I suppose it should exist a way to treat
} general purpose carbon fiber , which is generally insolating, to get it
} conducting, and have a cheaper way than the expensive genuine part (up
} to } 200. euro for 25 meter !).
}
} -For supplier : the same question about what other brand of fiber
} could be used. That supplied by Baltec is very thin, less then 1 mm in
} diameter. I had once some from a well known europeen TEM/SEM accessories
} supplier which was a real rope, nice for EPMA at high current, but much
} too thick for the power supply, and giving too to thick films for TEM or
} SEM imaging.
} -For europeen supplier : Who would have the Baltec referenced fiber
} on stock ? And at which price !!! (off list ?!)
}
} Thanks to all for help and answers.
}
} Jacques
}

==============================Original Headers==============================
9, 22 -- From dac-at-research.umass.edu Wed May 20 12:57:40 2009
9, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KHveo6016937
9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 12:57:40 -0500
9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
9, 22 -- (authenticated bits=0)
9, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4KHvd0B004970
9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 13:57:40 -0400
9, 22 -- Message-ID: {4A144498.6080107-at-research.umass.edu}
9, 22 -- Date: Wed, 20 May 2009 13:57:44 -0400
9, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
9, 22 -- Reply-To: dac-at-research.umass.edu
9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16
9, 22 -- MIME-Version: 1.0
9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 22 -- Subject: Re: [Microscopy] Carbon fiber for Baltec evaporator
9, 22 -- References: {200905201602.n4KG2DJt004548-at-ns.microscopy.com}
9, 22 -- In-Reply-To: {200905201602.n4KG2DJt004548-at-ns.microscopy.com}
9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: tina.williams-at-ars.usda.gov
Date: Wed, 20 May 2009 18:26:29 -0500
Subject: [Microscopy] viaWWW: cryo-ultramicrotome access or cryo-unit attachment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tina.williams-at-ars.usda.gov as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tina.williams-at-ars.usda.gov
Name: Tina Williams

Organization: U.S. Dept. of Agriculture

Title-Subject: [Microscopy] cryo-ultramicrotome access or cryo-unit attachment

Question: Hello,

Does anyone have an FS-1000 cryo attachment that they are using for
the RMC MT-6000 XL? If you have one you no longer use, we would like
to borrow it or buy one if possible. We need to do trial
cryo-ultramicrotome of a sample to see if it will work.

Thanks,

Tina Williams
USDA, ARS, Albany CA
510-559-6159


Login Host: 199.133.212.245
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed May 20 18:26:29 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KNQSkJ017921
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 18:26:29 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c63a421098bd-at-[206.69.208.22]}
10, 11 -- Date: Wed, 20 May 2009 18:26:27 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: tina.williams-at-ars.usda.gov (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: cryo-ultramicrotome access or cryo-unit attachment
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Thu, 21 May 2009 08:36:38 -0500
Subject: [Microscopy] SEM of silicon - question about polarity of doping

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues:

Suppose you have two pieces of silicon, doped as follows:
p-doped, conductivity/resistivity in the range 1-25 ohm-cm
n-doped, conductivity/resistivity in the range 1-25 ohm-cm

and coated as follows:
50-nm film of Aluminum, etched to form a nm-scale pattern.

Would you expect both specimens to image well in SEM at high magnification
(10kx-100kx) without charging artifacts?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
6, 23 -- From donc-at-asmicro.com Thu May 21 08:36:37 2009
6, 23 -- Received: from smtp117.sbc.mail.re3.yahoo.com (smtp117.sbc.mail.re3.yahoo.com [66.196.96.90])
6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4LDaZb2002864
6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 08:36:37 -0500
6, 23 -- Received: (qmail 52350 invoked from network); 21 May 2009 13:36:34 -0000
6, 23 -- Received: from unknown (HELO asm15) (donc-at-66.178.233.250 with login)
6, 23 -- by smtp117.sbc.mail.re3.yahoo.com with SMTP; 21 May 2009 13:36:33 -0000
6, 23 -- X-Yahoo-Newman-Property: ymail-3
6, 23 -- Message-ID: {8B3BF7CB1B524E89959B04D3CEB476E4-at-asm15}
6, 23 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
6, 23 -- To: "Microscopy List" {microscopy-at-microscopy.com}
6, 23 -- Subject: SEM of silicon - question about polarity of doping
6, 23 -- Date: Thu, 21 May 2009 09:34:02 -0400
6, 23 -- MIME-Version: 1.0
6, 23 -- Content-Type: text/plain;
6, 23 -- format=flowed;
6, 23 -- charset="iso-8859-1";
6, 23 -- reply-type=original
6, 23 -- Content-Transfer-Encoding: 7bit
6, 23 -- X-Priority: 3
6, 23 -- X-MSMail-Priority: Normal
6, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
6, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 21 May 2009 12:46:02 -0500
Subject: [Microscopy] Re: SEM of silicon - question about polarity of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues:

1. The Nanoprobe Network is hosting the third on-line Live Forum on
*Friday, May 22 from 11:30 AM to 1:00 PM, EDT. The discussion topic will
be “Piezoresponse Force Microscopy”. You are invited to post comments on
the Nanoprobe Network Forum anytime prior to, during, or after the event
(http://nanoprobenetwork.org/forum).

During the Live Forum, AFM experts from around the globe will discuss
issues, ideas, and questions regarding the applications, principles,
recent advances and technical know-how of piezoresponse force microscopy
of ferroelectrics, multiferroics, and biological systems. The questions
you will be able to ask can range from:

* how can I implement PFM on a microsope
* how do I distinguish the PFM image from cross-talk
* how to acquire high-resolution spectroscopic data , and how to
interpret it
* what are the advantages and pitfalls of resonance enhanced PFM
* can PFM be done in liquid
* and many others.

Special expert guests for this chat will be Dr. Sergei Kalinin from Oak
Ridge National Laboratory, Dr. Andrei Kholkin from the University of
Aveiro, Portugal, and Dr. Alexei Gruverman from the University of Nebraska.

(http://nanoprobenetwork.org/bbpress/forum.php?id=26)
This text-based forum allows any Nanoprobe Network member to ask and
answer questions, propose ideas, suggest literature, and explore any
topic of interest related to PFM. To participate, you must be a member
of the Nanoprobe Network. Registration is free. Simply go to
http://www.nanoprobenetwork.org, and click on the "Register" button in
the upper right.

2. Enter the Network’s Best SPM Image Contest! The submission deadline
is Friday, May 29, and the user who submits the winning image will
receive an iPod Nano©. The winner of our first contest, to choose a
slogan for the Network, was Dr. Jan-Willem Weener from SmartTip. His
winning slogan entry is: "Think small, look deeper." Dr. Weener’s iPod
Nano© prize was sponsored by RHK Technology (http://www.rhk-tech.com).
You can win the next iPod Nano by visiting
http://nanoprobenetwork.org/av-center/av-center-submission-instructions

We hope you find the Nanoprobe Network resource beneficial to your work
and we look forward to seeing you on-line!

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
12, 28 -- From sergei2-at-ornl.gov Thu May 21 12:02:56 2009
12, 28 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119])
12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4LH2tCq004631
12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 12:02:56 -0500
12, 28 -- Received: from emroute1.ornl.gov ([127.0.0.1])
12, 28 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
12, 28 -- with ESMTP id {0KK000H4C7CVA6-at-emroute1.ornl.gov} for
12, 28 -- microscopy-at-microscopy.com; Thu, 21 May 2009 13:02:55 -0400 (EDT)
12, 28 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov
12, 28 -- (PMDF V6.4 #31561) id {0KK000H017CVPD-at-emroute1.ornl.gov} ; Thu,
12, 28 -- 21 May 2009 13:02:55 -0400 (EDT)
12, 28 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
12, 28 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
12, 28 -- with ESMTP id {0KK000FB37CVXY-at-emroute1.ornl.gov} ; Thu,
12, 28 -- 21 May 2009 13:02:55 -0400 (EDT)
12, 28 -- Date: Thu, 21 May 2009 13:02:55 -0400
12, 28 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
12, 28 -- Subject: Piezoresponse Live Forum - May 22
12, 28 -- To: microscopy-at-microscopy.com
12, 28 -- Cc: Andrei Kholkin {kholkin-at-ua.pt} ,
12, 28 -- Alexei Gruverman {agruverman2-at-unlnotes.unl.edu} ,
12, 28 -- "Dawn A. Bonnell" {bonnell-at-lrsm.upenn.edu} ,
12, 28 -- "Robert W. Carpick" {carpick-at-seas.upenn.edu}
12, 28 -- Message-id: {4A15893F.5000808-at-ornl.gov}
12, 28 -- MIME-version: 1.0
12, 28 -- Content-type: text/plain; charset=windows-1252; format=flowed
12, 28 -- Content-transfer-encoding: 8BIT
12, 28 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
==============================End of - Headers==============================

From jonaggert-at-hotmail.com Thu May 21 12:39:29 2009
Return-Path: {jonaggert-at-hotmail.com}
Received: from google.com (aac176.neoplus.adsl.tpnet.pl [83.25.2.176])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4LHdQZT020420
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 21 May 2009 12:39:28 -0500
Received: from [151.70.44.142] (HELO google.com)
by lucky-gat.net; Thu, 21 May 2009 19:39:22 +0200
Message-ID: {00000006839EF36439486483}
Reply-To: Kezia Cross {1389jessica.broman-at-gmail.com}

If you mean p-type and n-type wafers then coated with Al,
I would think this would work. The wafers would have to
be cleaned and stripped of any oxide before putting down
the Al or it will charge.

gary g.



At 06:42 AM 5/21/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 21 -- From gary-at-gaugler.com Thu May 21 12:46:02 2009
9, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4LHk1Cf020859
9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 12:46:02 -0500
9, 21 -- Message-Id: {200905211746.n4LHk1Cf020859-at-ns.microscopy.com}
9, 21 -- Received: (qmail 8603 invoked from network); 21 May 2009 10:39:21 -0700
9, 21 -- Received: by simscan 1.1.0 ppid: 8600, pid: 8601, t: 0.1172s
9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 21 -- by smtp2 with SMTP; 21 May 2009 10:39:20 -0700
9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 21 -- Date: Thu, 21 May 2009 10:45:58 -0700
9, 21 -- To: donc-at-asmicro.com
9, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 21 -- Subject: Re: [Microscopy] SEM of silicon - question about polarity of
9, 21 -- doping
9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 21 -- In-Reply-To: {200905211342.n4LDgR83005522-at-ns.microscopy.com}
9, 21 -- References: {200905211342.n4LDgR83005522-at-ns.microscopy.com}
9, 21 -- Mime-Version: 1.0
9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: lesley.bechtold-at-jax.org
Date: Thu, 21 May 2009 21:02:57 -0500
Subject: [Microscopy] viaWWW: Cryo EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lesley.bechtold-at-jax.org as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lesley.bechtold-at-jax.org
Name: Lesley Bechtold

Organization: The Jackson Laboratory

Title-Subject: [Filtered] Cryo EM

Question: Good morning,

Does anyone have Balzers or Bal-Tec equipment
used for preparing cryo samples in the northeast
region and would they would be willing to provide
access to a colleague of mine from the University
of Maine? (his email is below) Please respond to
me directly. Thank you.

Lesley Bechtold

"Hi Lesley, thanks in advance for any assistance
you can provide in trying to locate the
instrument that I need to use. I need to rapidly
freeze aqueous solutions containing polymers such
that the water vitrifies. From what I see in the
literature the widely accepted means of doing
this is using liquid propane as the cryogen. The
sample is sandwiched between two copper
planchettes and either rapidly immersed into
liquid propane via a spring loaded device, or
sprayed with liquid nitrogen. The Balzers
company, now Bal-tec used to, and may still, make
these devices for Freeze Fracture etc studies. I
am hoping to find one in the North East that I
can use on a fee for service basis. If you know
of anyone that has one Iíd sincerely appreciate
the help.
Kind regards,
Dave"



Login Host: 209.222.206.50
---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Thu May 21 21:02:56 2009
12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4M22t8V028635
12, 13 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 21:02:56 -0500
12, 13 -- Mime-Version: 1.0
12, 13 -- Message-Id: {p06240800c63bb82f4974-at-[206.69.208.22]}
12, 13 -- Date: Thu, 21 May 2009 21:02:55 -0500
12, 13 -- To: microscopy-at-microscopy.com
12, 13 -- From: lesley.bechtold-at-jax.org (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Cryo EM
12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
12, 13 -- Content-Transfer-Encoding: 8bit
12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4M22t8V028635
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 22 May 2009 09:12:16 -0500
Subject: [Microscopy] Precipitates and 2-mercaptoethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There has been some interest in the procedures we use for getting rid of
osmium precipitates by using 2-mercaptoethanol in our specimen
processing procedures, so I have posted our actual processing worksheet
in Excel format on our website. You can link to it at
http://www.emc.missouri.edu/pandp.htm. Click on Microwave Processing
Worksheet.

Below is the formula we use to make 0.1M Na Cacodylate buffer with 0.13M
sucrose and 0.01M 2-Mercaptoethanol:

62.5 ml of 0.4M Na Cacodylate buffer
11.12 g sucrose
0.18 ml 2- Mercaptoethanol

Bring to 250ml with ultrapure water.

Obviously, you can change or eliminate the sucrose, as well as add
anything else that's compatible with the 2-Me.

Hope this useful to a few people.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
11, 27 -- From TindallR-at-missouri.edu Fri May 22 09:12:15 2009
11, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MECEKN010021
11, 27 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 09:12:15 -0500
11, 27 -- X-IronPort-Anti-Spam-Filtered: true
11, 27 -- X-IronPort-Anti-Spam-Result: ApoEABxPFkrRauUp/2dsb2JhbADBdgEJh3WIToJWgTUF
11, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
11, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 22 May 2009 09:12:10 -0500
11, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
11, 27 -- Fri, 22 May 2009 09:12:09 -0500
11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 27 -- Content-class: urn:content-classes:message
11, 27 -- MIME-Version: 1.0
11, 27 -- Content-Type: text/plain;
11, 27 -- charset="us-ascii"
11, 27 -- Subject: Precipitates and 2-mercaptoethanol
11, 27 -- Date: Fri, 22 May 2009 09:11:24 -0500
11, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8108-at-UM-XMAIL08.um.umsystem.edu}
11, 27 -- X-MS-Has-Attach:
11, 27 -- X-MS-TNEF-Correlator:
11, 27 -- Thread-Topic: Precipitates and 2-mercaptoethanol
11, 27 -- Thread-Index: Acna5zCPbLMsGhL3SOye1OX31Z4pyg==
11, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
11, 27 -- To: {microscopy-at-microscopy.com}
11, 27 -- X-OriginalArrivalTime: 22 May 2009 14:12:09.0948 (UTC) FILETIME=[4BB8E9C0:01C9DAE7]
11, 27 -- Content-Transfer-Encoding: 8bit
11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4MECEKN010021
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 22 May 2009 12:20:59 -0500
Subject: [Microscopy] ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
What is the highest pressure ESEM? Does any ESEM have as much as one
atmosphere?
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
2, 20 -- From tivol-at-caltech.edu Fri May 22 12:20:59 2009
2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MHKwY0001677
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 12:20:59 -0500
2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id A3B562E50D7A
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 10:20:58 -0700 (PDT)
2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
2, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id 91DEE2E5073D
2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 10:20:57 -0700 (PDT)
2, 20 -- Message-Id: {A64B037E-54B5-4B7E-97C1-E8CC1D83A9C1-at-caltech.edu}
2, 20 -- From: Bill Tivol {tivol-at-caltech.edu}
2, 20 -- To: microscopy-at-microscopy.com
2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
2, 20 -- Content-Transfer-Encoding: 7bit
2, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
2, 20 -- Subject: ESEM
2, 20 -- Date: Fri, 22 May 2009 10:20:55 -0700
2, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Fri, 22 May 2009 13:54:49 -0500
Subject: [Microscopy] ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I understood that FEI had the only true E-SEM since they took over
Electro-Scan. I recall the maximum pressure was 10 torr. That was enough
to maintain equilibrium with water vapor at room temperature. I seem to
recall the maximum partial pressure of water was around 5 torr at 20 C.
(I suppose I could look it up.)

What do you have in mind that you would need 1 atm?

For comparison, most VP-SEMs seem to max out at around 2 torr.

Warren S.

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Friday, May 22, 2009 12:22 PM
To: wesaia-at-iastate.edu

Dear List,
What is the highest pressure ESEM? Does any ESEM have as much
as one
atmosphere?
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




==============================Original Headers==============================
12, 36 -- From wesaia-at-iastate.edu Fri May 22 13:54:49 2009
12, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MIsnJq020000
12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:49 -0500
12, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
12, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4MIsmId020367
12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500
12, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
12, 36 -- id 04a1_067fda08_4702_11de_b35f_001372578af6;
12, 36 -- Fri, 22 May 2009 13:54:48 -0500
12, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
12, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n4MIsmuE011738
12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500
12, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
12, 36 -- Fri, 22 May 2009 13:54:48 -0500
12, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 36 -- Content-class: urn:content-classes:message
12, 36 -- MIME-Version: 1.0
12, 36 -- Content-Type: text/plain;
12, 36 -- charset="us-ascii"
12, 36 -- Subject: RE: [Microscopy] ESEM
12, 36 -- Date: Fri, 22 May 2009 13:56:01 -0500
12, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE8091-at-maire.eng.iastate.edu}
12, 36 -- In-Reply-To: {200905221721.n4MHLnT4002721-at-ns.microscopy.com}
12, 36 -- X-MS-Has-Attach:
12, 36 -- X-MS-TNEF-Correlator:
12, 36 -- Thread-Topic: [Microscopy] ESEM
12, 36 -- Thread-Index: AcnbAcwnkMtbRInoSrCiXF4/KNWO3QADLH2w
12, 36 -- References: {200905221721.n4MHLnT4002721-at-ns.microscopy.com}
12, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
12, 36 -- To: {Microscopy-at-microscopy.com}
12, 36 -- X-OriginalArrivalTime: 22 May 2009 18:54:48.0676 (UTC) FILETIME=[C7E97240:01C9DB0E]
12, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.22.183735
12, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='FROM_SAME_AS_TO 0.05, BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FROM_SAME_AS_TO2 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
12, 36 -- Content-Transfer-Encoding: 8bit
12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4MIsnJq020000
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Fri, 22 May 2009 16:00:52 -0500
Subject: [Microscopy] Greeting to those of you in the office

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Today's count was 13 automated out-of-office replies to my post of
earlier this afternoon. All but one of those was for people returning to
the office next Tuesday (or Monday for those outside the USA).

I'm always a little curious how many of those messages I am going to get
when I post. The number is up a little this time. Some of you must have
started the Memorial Day holiday weekend a little early.

Let me take the opportunity to remind everyone that the list guidelines
ask that people unsubscribe if they are going to engage an automated
out-of-office reply. Your replies got to everyone who posts to the list
as well as to your more personal correspondents.

Even though I have gotten to know some of you well enough that I might
even count you as friends, I still don't really care to know when you
are on vacation or traveling. {g}

To those of you who are in the office and in the US, may you enjoy the
extended weekend.

Warren S.


==============================Original Headers==============================
7, 34 -- From wesaia-at-iastate.edu Fri May 22 16:00:51 2009
7, 34 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ML0ogP006578
7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:51 -0500
7, 34 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
7, 34 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4ML0nqt031824
7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:49 -0500
7, 34 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-10.iastate.edu with smtp
7, 34 -- id 055b_a0f09ce2_4713_11de_801c_00137253420a;
7, 34 -- Fri, 22 May 2009 16:00:48 -0500
7, 34 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
7, 34 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4ML0l1R009381
7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:47 -0500
7, 34 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 34 -- Fri, 22 May 2009 16:00:48 -0500
7, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 34 -- Content-class: urn:content-classes:message
7, 34 -- MIME-Version: 1.0
7, 34 -- Content-Type: text/plain;
7, 34 -- charset="us-ascii"
7, 34 -- Subject: Greeting to those of you in the office
7, 34 -- Date: Fri, 22 May 2009 16:02:01 -0500
7, 34 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE80C4-at-maire.eng.iastate.edu}
7, 34 -- X-MS-Has-Attach:
7, 34 -- X-MS-TNEF-Correlator:
7, 34 -- Thread-Topic: Greeting to those of you in the office
7, 34 -- Thread-Index: AcnbII059FwfaeB+S+Wp1d4c6kGlIA==
7, 34 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
7, 34 -- To: {Microscopy-at-microscopy.com}
7, 34 -- X-OriginalArrivalTime: 22 May 2009 21:00:48.0487 (UTC) FILETIME=[61E90F70:01C9DB20]
7, 34 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.22.204638
7, 34 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_900_999 0, ECARD_GREETING_WORD 0, TO_NO_NAME 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
7, 34 -- Content-Transfer-Encoding: 8bit
7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4ML0ogP006578
==============================End of - Headers==============================




From: innap-at-savion.huji.ac.il
Date: Sat, 23 May 2009 07:28:22 -0500
Subject: [Microscopy] viaWWW: ESEM - Max pressure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both innap-at-savion.huji.ac.il as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: innap-at-savion.huji.ac.il
Name: Inna Popov

Organization: Hebrew University of Jerusalem

Title-Subject: [Filtered] ESEM - Max pressure

Question: Hi,
As well as I know the max pressure in ESEM mode (FEI SEM) depends on
the PLA - pressure limiting aperture, and could be 40 or 20 Torr.
Sure, to realize the ESEM at such high pressure cooling of the sample
is obligatory. Therefore small Peltier attachment to stage MUST
present as a part of setup for ESEM experiment.
Best,
Inna

Login Host: 89.139.153.173
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Sat May 23 07:28:22 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4NCSKN1018707
6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 23 May 2009 07:28:22 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c63d9c53c418-at-[206.69.208.22]}
6, 11 -- Date: Sat, 23 May 2009 07:28:19 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: innap-at-savion.huji.ac.il (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: ESEM - Max pressure
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Sat, 23 May 2009 20:16:33 -0500
Subject: [Microscopy] ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Zeiss, who bought out Cambridge, have a range of ESEMs - we purchased the
EVO LS 15 last year. Much cheaper than FEI though not up to FEI
performance, but will image material in up to a few Torr (had to look this
up, work only in Pa these days...). Unfortunately, it won't image material
cooled on the Peltier stage with water vapour in the chamber (as claimed)
unless you go to higher vacuum - the low-vacuum detector is not up to
scratch yet. For ESEM at lowest vacuum you'd need one of the FEI Quanta
range, but I don't imagine you'd be able to see anything much at 1
atmosphere, the fog would be too thick.
cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 23/05/09 5:00 AM, "wesaia-at-iastate.edu" {wesaia-at-iastate.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I understood that FEI had the only true E-SEM since they took over
} Electro-Scan. I recall the maximum pressure was 10 torr. That was enough
} to maintain equilibrium with water vapor at room temperature. I seem to
} recall the maximum partial pressure of water was around 5 torr at 20 C.
} (I suppose I could look it up.)
}
} What do you have in mind that you would need 1 atm?
}
} For comparison, most VP-SEMs seem to max out at around 2 torr.
}
} Warren S.
}
} -----Original Message-----
} X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
} Sent: Friday, May 22, 2009 12:22 PM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] ESEM
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} Dear List,
} What is the highest pressure ESEM? Does any ESEM have as much
} as one
} atmosphere?
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
}
} ==============================Original Headers==============================
} 12, 36 -- From wesaia-at-iastate.edu Fri May 22 13:54:49 2009
} 12, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu
} [129.186.140.15])
} 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n4MIsnJq020000
} 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:49 -0500
} 12, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu
} [129.186.1.48])
} 12, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id
} n4MIsmId020367
} 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500
} 12, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by
} devirus-11.iastate.edu with smtp
} 12, 36 -- id 04a1_067fda08_4702_11de_b35f_001372578af6;
} 12, 36 -- Fri, 22 May 2009 13:54:48 -0500
} 12, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
} [129.186.23.85])
} 12, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id
} n4MIsmuE011738
} 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500
} 12, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
} owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 12, 36 -- Fri, 22 May 2009 13:54:48 -0500
} 12, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 12, 36 -- Content-class: urn:content-classes:message
} 12, 36 -- MIME-Version: 1.0
} 12, 36 -- Content-Type: text/plain;
} 12, 36 -- charset="us-ascii"
} 12, 36 -- Subject: RE: [Microscopy] ESEM
} 12, 36 -- Date: Fri, 22 May 2009 13:56:01 -0500
} 12, 36 -- Message-ID:
} {16A330AC32056A40B32842EC4BB8D72703DE8091-at-maire.eng.iastate.edu}
} 12, 36 -- In-Reply-To: {200905221721.n4MHLnT4002721-at-ns.microscopy.com}
} 12, 36 -- X-MS-Has-Attach:
} 12, 36 -- X-MS-TNEF-Correlator:
} 12, 36 -- Thread-Topic: [Microscopy] ESEM
} 12, 36 -- Thread-Index: AcnbAcwnkMtbRInoSrCiXF4/KNWO3QADLH2w
} 12, 36 -- References: {200905221721.n4MHLnT4002721-at-ns.microscopy.com}
} 12, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 12, 36 -- To: {Microscopy-at-microscopy.com}
} 12, 36 -- X-OriginalArrivalTime: 22 May 2009 18:54:48.0676 (UTC)
} FILETIME=[C7E97240:01C9DB0E]
} 12, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912,
} Antispam-Data: 2009.5.22.183735
} 12, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
} Report='FROM_SAME_AS_TO 0.05, BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0,
} BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0,
} __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY
} 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FROM_SAME_AS_TO2 0, __HAS_MSGID 0,
} __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0,
} __SANE_MSGID 0, __TO_MALFORMED_2 0'
} 12, 36 -- Content-Transfer-Encoding: 8bit
} 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4MIsnJq020000
} ==============================End of - Headers==============================



==============================Original Headers==============================
8, 43 -- From prvs=388877f58=Rosemary.White-at-csiro.au Sat May 23 20:16:33 2009
8, 43 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43])
8, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4O1GV5k004223
8, 43 -- for {Microscopy-at-microscopy.com} ; Sat, 23 May 2009 20:16:32 -0500
8, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
8, 43 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
8, 43 -- s=email; t=1243127792; x=1274663792;
8, 43 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
8, 43 -- mime-version:content-transfer-encoding:content-id:
8, 43 -- content-description:resent-date:resent-from:resent-sender:
8, 43 -- resent-to:resent-cc:resent-message-id:in-reply-to:
8, 43 -- references:list-id:list-help:list-unsubscribe:
8, 43 -- list-subscribe:list-post:list-owner:list-archive;
8, 43 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
8, 43 -- |Subject:=20Re:=20[Microscopy]=20RE:=20ESEM|Date:=20Sun,
8, 43 -- =2024=20May=202009=2011:16:25=20+1000|Message-ID:=20 {C63E
8, 43 -- DD09.5E51%rosemary.white-at-csiro.au} |To:=20 {Microscopy-at-micr
8, 43 -- oscopy.com} |MIME-Version:=201.0
8, 43 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200905
8, 43 -- 221900.n4MJ0BNA029014-at-ns.microscopy.com} ;
8, 43 -- bh=lrqwb3QQmVBKRQulBvIxA6ZN08pB4BhnzI9IYpFGqVM=;
8, 43 -- b=GVqJWheId9uG8+PTXNTJucuZrIHKMnK86rpIHyGvO+BoFdv7TDOb7Dgs
8, 43 -- zPuVi0J2V8xt4tmtdRHdDie0GPGvMxtiRz45v2eNIk+Go1NvLR/GW4pYJ
8, 43 -- Mx+63TcU4W+ix2u;
8, 43 -- X-IronPort-AV: E=Sophos;i="4.41,238,1241359200";
8, 43 -- d="scan'208";a="27224728"
8, 43 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
8, 43 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 24 May 2009 11:16:29 +1000
8, 43 -- Received: from [152.83.193.49] (152.83.193.49) by exvic-htca01.nexus.csiro.au
8, 43 -- (138.194.81.126) with Microsoft SMTP Server id 8.1.358.0; Sun, 24 May 2009
8, 43 -- 11:16:29 +1000
8, 43 -- User-Agent: Microsoft-Entourage/12.10.0.080409
8, 43 -- Date: Sun, 24 May 2009 11:16:25 +1000
8, 43 -- Subject: Re: [Microscopy] RE: ESEM
8, 43 -- From: Rosemary White {rosemary.white-at-csiro.au}
8, 43 -- To: {Microscopy-at-microscopy.com}
8, 43 -- Message-ID: {C63EDD09.5E51%rosemary.white-at-csiro.au}
8, 43 -- Thread-Topic: [Microscopy] RE: ESEM
8, 43 -- Thread-Index: AcnbD5AhdrSMpXKeRJ2ujtGlan4vGwA/bF0l
8, 43 -- In-Reply-To: {200905221900.n4MJ0BNA029014-at-ns.microscopy.com}
8, 43 -- MIME-Version: 1.0
8, 43 -- Content-Type: text/plain; charset="US-ASCII"
8, 43 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 26 May 2009 04:54:37 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

Independently of the main message of your mail,  I think it is a mistake to calculate in 3D even though we are talking about gas here.
This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example.

Regards,
Stephane



----- Original Message ----
X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, May 20, 2009 3:04:22 PM

John:

    Are you seriously stirring this up again?  Didn't we just handle O2
displacement via nitrogen boil off a few weeks ago?

    The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only
300 cubic feet of nitrogen.  That's a space 6.7' x 6.7' x 6.7' at
100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a
tiny room for a scope and all the N2 would have to be released
rapidly enough to displace the O2 in the room (or reduce it from 22%
to below 12% for this to become an issue let alone life threatening)
without the N2 leaking out the doorway or any O2 leaking in.  But in
fact it sounds like ". . .going through nitrogen gas like there is no
tomorrow."  is probably days not minutes, and the scope room is a
"normal room" and not a sealed environmental chamber.

    I have worked with compressed atmospheric air.  No matter how dry it
is an extremely corrosive material, degrading fittings, seals and
regulator parts.  I would suggest the long term costs and safety make
working with compressed air a far worse choice than ppN2


On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor:  The Microscopy Society
} of America To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Elgin;
} My I suggest also that you have created a significant safety hazard by
}   running your air table, and I presume your valving, on nitrogen. The
} leak you now have (and everything develops leaks eventually) is
} filling up your room with pure nitrogen and displacing the oxygen. You
}   should be using only clean dry air for these items so that when it
} leaks, the room fills up with.....air! Air that you can breath!
} Nitrogen should only be used for venting the chamber.
}
}
} Sent from my iPhone.
} John Mardinly
}
} On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
}
} }
} }
} }
} } ---
} } ---
} } --------------------------------------------------------------------
} } -- The Microscopy ListServer -- CoSponsor:  The Microscopy Society
} } of  America To  Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help


Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712        Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


==============================Original Headers==============================
10, 25 -- From edelmare-at-muohio.edu Wed May 20 07:53:35 2009
10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.67])
10, 25 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KCrZNA004846
10, 25 --     for {microscopy-at-Microscopy.com} ; Wed, 20 May 2009 07:53:35 -0500
10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
10, 25 --     by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrsGt014924;
10, 25 --     Wed, 20 May 2009 08:53:54 -0400
10, 25 -- Received: from [192.168.1.23] ([134.53.14.105])
10, 25 --     by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrYF2021966;
10, 25 --     Wed, 20 May 2009 08:53:34 -0400
10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
10, 25 -- To: microscopy-at-Microscopy.com
10, 25 -- Date: Wed, 20 May 2009 08:53:34 -0400
10, 25 -- MIME-Version: 1.0
10, 25 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak
10, 25 -- CC: "jmardinly-at-gmail.com" {jmardinly-at-gmail.com}
10, 25 -- Message-ID: {4A13C50E.27113.A79CE8A-at-edelmare.muohio.edu}
10, 25 -- Priority: normal
10, 25 -- In-reply-to: {200905082112.n48LCJEb013069-at-ns.microscopy.com}
10, 25 -- References: {200905082112.n48LCJEb013069-at-ns.microscopy.com}
10, 25 -- X-mailer: Pegasus Mail for Windows (4.41)
10, 25 -- Content-type: text/plain; charset=US-ASCII
10, 25 -- Content-transfer-encoding: 7BIT
10, 25 -- Content-description: Mail message body
10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67
==============================End of - Headers==============================






==============================Original Headers==============================
26, 25 -- From nizets2-at-yahoo.com Tue May 26 04:54:37 2009
26, 25 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
26, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4Q9sa0L026752
26, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 04:54:36 -0500
26, 25 -- Received: (qmail 78109 invoked by uid 60001); 26 May 2009 09:54:35 -0000
26, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243331675; bh=FPihZg+VPIBsWzBKW7S9flOM9f7OL/wjPlgVw1cGIKQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=UM7N13p3BBmsfnFFw6qvwHkvCqRYdPOEawnl2xMAba5rZO0zLFQU6d2DXPo6ikOJLC6lxnHlzOuBUZPiWCYF01kAZggA9W2XEsDcf9sLAu20Mkayo6Dg/rft3fNKunfrKKUTjEbtJlOyqzZ3Tt2rvUvmr7y6moUb5DAtOULH1DQ=
26, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
26, 25 -- s=s1024; d=yahoo.com;
26, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
26, 25 -- b=OnpWwjWCf07a3DDVUnu6CeTOkGa+kG9Rsgy2J/SWbm+mb/yRreYvdFXdajZ0VHioG5LxhDmzgJaH6/6qMwXIPxXu4/oFVXg1d8+g9Mn127Ul29Oe639nSe/bc+oS/FA55ALnK6gJFdOS/v7ccYosQRJZxzfasq+hmaatbGeKvnk=;
26, 25 -- Message-ID: {649345.78052.qm-at-web110804.mail.gq1.yahoo.com}
26, 25 -- X-YMail-OSG: JAeLrdEVM1lQq.nCnfKIL2Iol4eVVudxaAYM8H2AkrjFb5iHjCBxvGFbxmID.MIr0BLUKWjHzFJudvLiTeaDuBf7m4QqMK6nAKPxGz6zl78Jd2oDIpJzJ0YsSyUeyb1ACOYruODGsF5i1VviXD.oT6vJATUdBlzdOQ.BAJ87JnWvJ.EKDLgrZNNTNNWgXvKHV04TJBAZoAUX5cw_b8Q_c3Xt7x8bIooh_vt7zggHnfUC6VGzE5Z_wR_5xA9iGXTXDb6D4JYh3zs.w0reKTHZeLIevMwe9Pp1w1Htor_oQaw1dw9eX9z2MwpNA1rRLTXfkUcoysnTkyDWS.m3JA--
26, 25 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 26 May 2009 02:54:35 PDT
26, 25 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
26, 25 -- References: {200905201304.n4KD4MU5011688-at-ns.microscopy.com}
26, 25 -- Date: Tue, 26 May 2009 02:54:35 -0700 (PDT)
26, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
26, 25 -- Subject: Re: [Microscopy] viaWWW: Nitrogen leak
26, 25 -- To: edelmare-at-muohio.edu
26, 25 -- Cc: microscopy-at-microscopy.com
26, 25 -- In-Reply-To: {200905201304.n4KD4MU5011688-at-ns.microscopy.com}
26, 25 -- MIME-Version: 1.0
26, 25 -- Content-Type: text/plain; charset=iso-8859-1
26, 25 -- Content-Transfer-Encoding: 8bit
26, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4Q9sa0L026752
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 26 May 2009 09:07:40 -0500
Subject: [Microscopy] The Redox properties of minerals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all!

This subject is partly relevant to electron microscopy since TEM and SEM are parts of the analysis procedure.
I am interested in the redox properties of some minerals. Some minerals (namely layered silicates but I also read something about amphiboles) seem to be able to reduce metals in solutions, leading to their precipitation. The precipitation process does not occur uniformly at the surface of the minerals but is dependent on the structure.
A paper of particular interest is "Reaction of some trioctahedral micas with copper sulfate solutions at 25°C and 1 atm; an electron probe and TEM investigation" by Ilton et al. (1992).

Unfortunately, being a biologist I have no access to papers in earth science (which demonstrates the sad partitioning in science), not even the paper I cited above.

I would like to know the procedure used by these authors (or others). I also read about the possibility to precipitate silver ions on biotite but I need more details.

So I send this message in the bottle to the list. This is a call to anybody in the field who could help me devise a protocol, or simply send me a copy of the above paper. My knowledge in mineralogy is limited (I have some background), so I would appreciate being able to discuss specific issues like which kind a mineral would be expected to have which redox property...

Best regards,

Stephane-without-a-i





==============================Original Headers==============================
11, 22 -- From nizets2-at-yahoo.com Tue May 26 09:07:40 2009
11, 22 -- Received: from web110806.mail.gq1.yahoo.com (web110806.mail.gq1.yahoo.com [67.195.13.229])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4QE7dUD024382
11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:07:39 -0500
11, 22 -- Received: (qmail 49096 invoked by uid 60001); 26 May 2009 14:07:38 -0000
11, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243346857; bh=UlO355lMNinYx75gKjClgVcwWXrtZY/bkCiR8ploQeI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jRjj0vTZ+6RwYXxpYFweMBoXZgKGmtG5Hkq57FmsXUw38N0gHLBU7xpWo9pO1afJixXQSI2qWatEQhSJDi49mztFVnAO8FXcefZCviLS2On/oLcksUVrAptXKohJhVf0683UY2Kkxe2JCf52/BVytPTiQCiqkI+FNvHb7/RSNS8=
11, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
11, 22 -- s=s1024; d=yahoo.com;
11, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
11, 22 -- b=EfO94aDLWTRWAcrgkuYhA4UGGq46blHjxPR/8zukczi3SqE4QvO49dXpdwhcU3soKzRViPTo304kmxfkNiDtVeB8rUz/wgYrOuuQtGJ2erWhf60cGc4XAVfrl2hlFf+KaUHnsQRn54/M+2FbICo78+pqxWnPePPHIjV3/LP9faY=;
11, 22 -- Message-ID: {935923.48714.qm-at-web110806.mail.gq1.yahoo.com}
11, 22 -- X-YMail-OSG: CiWDi6UVM1mEEbRCcnB3FZ1_nN8UrSwB7HDmYb0QyLC206tlL_IH9C5MEd0lDc04MVmDdjoas49TAbJftX9.mFg104ecaEqIzr7Ru2xujiL0S6t.nZO3LPfA9L6XDWKhsyNfUaDDtaHIZQmP4srGj_hbunzZ.gNFNSkjfurh6lG5R9CizZfXwU.Yqbt4yz36UqaYMwtaGuvCX4N7e9Fgp6lz5_mcye1IiTpuQmD9QEihY5kn2ml5UYnjEmdKDEwnFqqlQRWwVu_NN.hr6GVDN57IISGKE70z03GCi_7BnI1kpeh8yIj4Mw--
11, 22 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Tue, 26 May 2009 07:07:37 PDT
11, 22 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
11, 22 -- Date: Tue, 26 May 2009 07:07:37 -0700 (PDT)
11, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
11, 22 -- Subject: The Redox properties of minerals
11, 22 -- To: microscopy-at-microscopy.com
11, 22 -- MIME-Version: 1.0
11, 22 -- Content-Type: text/plain; charset=iso-8859-1
11, 22 -- Content-Transfer-Encoding: 8bit
11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QE7dUD024382
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Tue, 26 May 2009 09:20:57 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are you sure the nitrogen will accumulate near the floor? This issue was about compressed nitrogen gas rather than LN2. Has anyone shown that this is an issue that we should be concerned about?

The N2 would cool a little upon expansion, but the flow would be slow enough and the cooling is not so drastic that I would not think a layer of cool gas would build up on the floor. Once warmed to room temperature, the N2 would be ever so slightly lighter than air. It seems this _should_ be treated as a 3-D dilution problem.

Warren


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, May 26, 2009 4:56 AM
To: wesaia-at-iastate.edu

Hi!

Independently of the main message of your mail,  I think it is a mistake to calculate in 3D even though we are talking about gas here.
This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example.

Regards,
Stephane

----- Original Message ----
X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, May 20, 2009 3:04:22 PM

John:

    Are you seriously stirring this up again?  Didn't we just handle O2
displacement via nitrogen boil off a few weeks ago?

    The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only
300 cubic feet of nitrogen.  That's a space 6.7' x 6.7' x 6.7' at
100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a
tiny room for a scope and all the N2 would have to be released
rapidly enough to displace the O2 in the room (or reduce it from 22%
to below 12% for this to become an issue let alone life threatening)
without the N2 leaking out the doorway or any O2 leaking in.  But in
fact it sounds like ". . .going through nitrogen gas like there is no
tomorrow."  is probably days not minutes, and the scope room is a
"normal room" and not a sealed environmental chamber.

    I have worked with compressed atmospheric air.  No matter how dry it
is an extremely corrosive material, degrading fittings, seals and
regulator parts.  I would suggest the long term costs and safety make
working with compressed air a far worse choice than ppN2


On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor:  The Microscopy Society
} of America To  Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Elgin;
} My I suggest also that you have created a significant safety hazard by
}   running your air table, and I presume your valving, on nitrogen. The
} leak you now have (and everything develops leaks eventually) is
} filling up your room with pure nitrogen and displacing the oxygen. You
}   should be using only clean dry air for these items so that when it
} leaks, the room fills up with.....air! Air that you can breath!
} Nitrogen should only be used for venting the chamber.
}
}
} Sent from my iPhone.
} John Mardinly
}
} On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
}
} }
} }
} }
} } ---
} } ---
} } --------------------------------------------------------------------
} } -- The Microscopy ListServer -- CoSponsor:  The Microscopy Society
} } of  America To  Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help



==============================Original Headers==============================
20, 36 -- From wesaia-at-iastate.edu Tue May 26 09:20:57 2009
20, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
20, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEKsRQ002148
20, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:20:55 -0500
20, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
20, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4QEKrpT006037;
20, 36 -- Tue, 26 May 2009 09:20:53 -0500
20, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
20, 36 -- id 0c88_6bdcb654_4a00_11de_a285_001372578af6;
20, 36 -- Tue, 26 May 2009 09:20:53 -0500
20, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
20, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4QEKqN3016931;
20, 36 -- Tue, 26 May 2009 09:20:52 -0500
20, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
20, 36 -- Tue, 26 May 2009 09:20:52 -0500
20, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 36 -- Content-class: urn:content-classes:message
20, 36 -- MIME-Version: 1.0
20, 36 -- Content-Type: text/plain;
20, 36 -- charset="iso-8859-1"
20, 36 -- Subject: RE: [Microscopy] Re: viaWWW: Nitrogen leak
20, 36 -- Date: Tue, 26 May 2009 09:22:07 -0500
20, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703E4A06B-at-maire.eng.iastate.edu}
20, 36 -- In-Reply-To: {200905260956.n4Q9u554028058-at-ns.microscopy.com}
20, 36 -- X-MS-Has-Attach:
20, 36 -- X-MS-TNEF-Correlator:
20, 36 -- Thread-Topic: [Microscopy] Re: viaWWW: Nitrogen leak
20, 36 -- Thread-Index: Acnd6DD2Rgqp93I5SXyQuSYC2FQUAQAI46fg
20, 36 -- References: {200905260956.n4Q9u554028058-at-ns.microscopy.com}
20, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
20, 36 -- To: {nizets2-at-yahoo.com} , {Microscopy-at-microscopy.com}
20, 36 -- X-OriginalArrivalTime: 26 May 2009 14:20:52.0231 (UTC) FILETIME=[2CADF970:01C9DE0D]
20, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.26.141105
20, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0'
20, 36 -- Content-Transfer-Encoding: 8bit
20, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEKsRQ002148
==============================End of - Headers==============================




From: ph2-at-sprynet.com
Date: Tue, 26 May 2009 09:45:50 -0500
Subject: [Microscopy] The Redox properties of minerals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

1. Go to:

http://homepages.eawag.ch/~wehrli/papers/87_97/Wehrli_RedoxSurface_Wiley90.p
df

2. Look also for:

Stumm, W. : Chemistry of the solid-water interface: Processes at the
mineral-water and particle-water interface in natural systems. John Wiley
Sons, 605 Third Ave., New York, NY 10158-0012 (United States). 1992

3 And probably the best thing:

Do a search on Metal Oxide - Mica Pigments as these as newer Luster pigments
created by coating metal oxides onto mica (muscovite/biotite).


Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
www.ph2llc.com

(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, May 26, 2009 10:18 AM
To: ph2-at-sprynet.com


Hi all!

This subject is partly relevant to electron microscopy since TEM and SEM are
parts of the analysis procedure.
I am interested in the redox properties of some minerals. Some minerals
(namely layered silicates but I also read something about amphiboles) seem
to be able to reduce metals in solutions, leading to their precipitation.
The precipitation process does not occur uniformly at the surface of the
minerals but is dependent on the structure.
A paper of particular interest is "Reaction of some trioctahedral micas with
copper sulfate solutions at 25°C and 1 atm; an electron probe and TEM
investigation" by Ilton et al. (1992).

Unfortunately, being a biologist I have no access to papers in earth science
(which demonstrates the sad partitioning in science), not even the paper I
cited above.

I would like to know the procedure used by these authors (or others). I also
read about the possibility to precipitate silver ions on biotite but I need
more details.

So I send this message in the bottle to the list. This is a call to anybody
in the field who could help me devise a protocol, or simply send me a copy
of the above paper. My knowledge in mineralogy is limited (I have some
background), so I would appreciate being able to discuss specific issues
like which kind a mineral would be expected to have which redox property...

Best regards,

Stephane-without-a-i





==============================Original Headers==============================
11, 22 -- From nizets2-at-yahoo.com Tue May 26 09:07:40 2009
11, 22 -- Received: from web110806.mail.gq1.yahoo.com
(web110806.mail.gq1.yahoo.com [67.195.13.229])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n4QE7dUD024382
11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:07:39
-0500
11, 22 -- Received: (qmail 49096 invoked by uid 60001); 26 May 2009 14:07:38
-0000
11, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1243346857; bh=UlO355lMNinYx75gKjClgVcwWXrtZY/bkCiR8ploQeI=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
b=jRjj0vTZ+6RwYXxpYFweMBoXZgKGmtG5Hkq57FmsXUw38N0gHLBU7xpWo9pO1afJixXQSI2qWa
tEQhSJDi49mztFVnAO8FXcefZCviLS2On/oLcksUVrAptXKohJhVf0683UY2Kkxe2JCf52/BVytP
TiQCiqkI+FNvHb7/RSNS8=
11, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
11, 22 -- s=s1024; d=yahoo.com;
11, 22 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
11, 22 --
b=EfO94aDLWTRWAcrgkuYhA4UGGq46blHjxPR/8zukczi3SqE4QvO49dXpdwhcU3soKzRViPTo30
4kmxfkNiDtVeB8rUz/wgYrOuuQtGJ2erWhf60cGc4XAVfrl2hlFf+KaUHnsQRn54/M+2FbICo78+
pqxWnPePPHIjV3/LP9faY=;
11, 22 -- Message-ID: {935923.48714.qm-at-web110806.mail.gq1.yahoo.com}
11, 22 -- X-YMail-OSG:
CiWDi6UVM1mEEbRCcnB3FZ1_nN8UrSwB7HDmYb0QyLC206tlL_IH9C5MEd0lDc04MVmDdjoas49T
AbJftX9.mFg104ecaEqIzr7Ru2xujiL0S6t.nZO3LPfA9L6XDWKhsyNfUaDDtaHIZQmP4srGj_hb
unzZ.gNFNSkjfurh6lG5R9CizZfXwU.Yqbt4yz36UqaYMwtaGuvCX4N7e9Fgp6lz5_mcye1IiTpu
QmD9QEihY5kn2ml5UYnjEmdKDEwnFqqlQRWwVu_NN.hr6GVDN57IISGKE70z03GCi_7BnI1kpeh8
yIj4Mw--
11, 22 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com
via HTTP; Tue, 26 May 2009 07:07:37 PDT
11, 22 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
11, 22 -- Date: Tue, 26 May 2009 07:07:37 -0700 (PDT)
11, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
11, 22 -- Subject: The Redox properties of minerals
11, 22 -- To: microscopy-at-microscopy.com
11, 22 -- MIME-Version: 1.0
11, 22 -- Content-Type: text/plain; charset=iso-8859-1
11, 22 -- Content-Transfer-Encoding: 8bit
11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n4QE7dUD024382
==============================End of - Headers==============================



==============================Original Headers==============================
29, 29 -- From ph2-at-sprynet.com Tue May 26 09:45:50 2009
29, 29 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62])
29, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEjmYx021375
29, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:45:49 -0500
29, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
29, 29 -- s=dk20050327; d=sprynet.com;
29, 29 -- b=YmDlvrDd8Mbi1RO0nj87At7seDRMhO6+EFTHHjGL3CKB9I4b9Jds1XSJYIttJtFj;
29, 29 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP;
29, 29 -- Received: from [75.61.18.94] (helo=user915fa8f284)
29, 29 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
29, 29 -- (envelope-from {ph2-at-sprynet.com} )
29, 29 -- id 1M8xuS-0007Ns-FH; Tue, 26 May 2009 10:45:44 -0400
29, 29 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
29, 29 -- To: {nizets2-at-yahoo.com}
29, 29 -- Cc: "Microscopy Listserve" {microscopy-at-microscopy.com}
29, 29 -- Subject: RE: [Microscopy] The Redox properties of minerals
29, 29 -- Date: Tue, 26 May 2009 10:45:39 -0400
29, 29 -- MIME-Version: 1.0
29, 29 -- Content-Type: text/plain;
29, 29 -- charset="iso-8859-1"
29, 29 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
29, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
29, 29 -- Thread-Index: AcneDMTU0fXg8Vp9RMioRwZrmJ1fegAAvx1g
29, 29 -- In-Reply-To: {200905261417.n4QEHt3O031880-at-ns.microscopy.com}
29, 29 -- Message-ID: {E1M8xuS-0007Ns-FH-at-elasmtp-dupuy.atl.sa.earthlink.net}
29, 29 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f94068f1d8be47278b1331ba7cc94569b6350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
29, 29 -- X-Originating-IP: 75.61.18.94
29, 29 -- Content-Transfer-Encoding: 8bit
29, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEjmYx021375
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Tue, 26 May 2009 10:16:40 -0500
Subject: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a problem?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

I haven't followed this whole thread, but I keep seeing messages pop up
on this topic. Maybe I missed something but is this really an issue? A
"standard" "200" cylinder of N2 (70 lbs, 2490psi) is listed as
containing 255cu ft (at STP, I assume, I didn't do the math...) and
this is only ~1/3 the volume of a 10ftx10ft room (8 ft ceilings,
neglecting installed content volume) even if it were instantly allowed
to fill the room; yes this would make the reduced O2 availability
equivalent to the "death zone" of mountain climbing
(http://en.wikipedia.org/wiki/Death_zone), although in that case O2
availability is reduced due to reduced pressure, not simply
concentration.... But death zone death is not instantaneous and you
would probably get loopy and sick but have enough wits to get to fresh
air. Most people would be well aware of a leak of that magnitude (would
sound like a deafening explosive "whoosh" that would interrupt even my
deepest nap). Rooms should have some ventillation that will draw in
fresh air. If I am going through a tank a month, I consider this a leak
that needs attention - because of trouble and expense more than other
reasons.

Most microscope rooms are probably something like 10'x10' for practical
reasons. If we only connect one tank at a time, it limits the risk. Or
else, use of a smaller tank will reduce the risk further. Make sure you
have code-compliant ventilation - a good idea anyway*.

So it this really a problem worth this level of concern? Has anyone ever
had a problem or been harmed?

Dale


*Ventilation can be an issue with TEM specs. A microscope I am familiar
with specs an air flow limitation that precludes breathing by the
operator. I haven't seen data for other manufacturers.




wesaia-at-iastate.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Are you sure the nitrogen will accumulate near the floor? This issue was about compressed nitrogen gas rather than LN2. Has anyone shown that this is an issue that we should be concerned about?
}
} The N2 would cool a little upon expansion, but the flow would be slow enough and the cooling is not so drastic that I would not think a layer of cool gas would build up on the floor. Once warmed to room temperature, the N2 would be ever so slightly lighter than air. It seems this _should_ be treated as a 3-D dilution problem.
}
} Warren
}
}
} -----Original Message-----
} X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
} Sent: Tuesday, May 26, 2009 4:56 AM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Re: viaWWW: Nitrogen leak
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi!
}
} Independently of the main message of your mail, I think it is a mistake to calculate in 3D even though we are talking about gas here.
} This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example.
}
} Regards,
} Stephane
}
} ----- Original Message ----
} X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, May 20, 2009 3:04:22 PM
} Subject: [Microscopy] viaWWW: Nitrogen leak
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} John:
}
} Are you seriously stirring this up again? Didn't we just handle O2
} displacement via nitrogen boil off a few weeks ago?
}
} The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only
} 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at
} 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a
} tiny room for a scope and all the N2 would have to be released
} rapidly enough to displace the O2 in the room (or reduce it from 22%
} to below 12% for this to become an issue let alone life threatening)
} without the N2 leaking out the doorway or any O2 leaking in. But in
} fact it sounds like ". . .going through nitrogen gas like there is no
} tomorrow." is probably days not minutes, and the scope room is a
} "normal room" and not a sealed environmental chamber.
}
} I have worked with compressed atmospheric air. No matter how dry it
} is an extremely corrosive material, degrading fittings, seals and
} regulator parts. I would suggest the long term costs and safety make
} working with compressed air a far worse choice than ppN2
}
}
} On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:
}
} }
} }
} } ----------------------------------------------------------------------
} } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } Elgin;
} } My I suggest also that you have created a significant safety hazard by
} } running your air table, and I presume your valving, on nitrogen. The
} } leak you now have (and everything develops leaks eventually) is
} } filling up your room with pure nitrogen and displacing the oxygen. You
} } should be using only clean dry air for these items so that when it
} } leaks, the room fills up with.....air! Air that you can breath!
} } Nitrogen should only be used for venting the chamber.
} }
} }
} } Sent from my iPhone.
} } John Mardinly
} }
} } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
} }
} } }
} } }
} } } ---
} } } ---
} } } --------------------------------------------------------------------
} } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society
} } } of America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html --- ---
} } } --------------------------------------------------------------------
} } } --
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at
} } } http://www.microscopy.com/MLFormMail.html --- ---
} } } --------------------------------------------------------------------
} } } - Remember this posting is most likely not from a Subscriber, so
} } } when replying please copy both eawoodruff-at-live.com as well as
} } } the MIcroscopy Listserver --- ---
} } } --------------------------------------------------------------------
} } } -
} } }
} } } Email: eawoodruff-at-live.com
} } } Name: Elvin Woodruff III
} } }
} } } Organization: Tennessee State University
} } }
} } } Title-Subject: [Filtered] Nitrogen leak
} } }
} } } Question: Hi All,
} } }
} } } I have recently started working with a JEOL 6701F scanning electron
} } } microscopy. The scope is running just fine, but it is going through
} } } nitrogen gas like there is not tomorrow. The scope sits on an air
} } } table which is supplied by gas (could this be it?)and other areas
} } } such as the specimen load chamber is also filled with nitrogen gas.
} } } I have talked to a couple of other people about the gas loss but to
} } } no avail. I have checked all of the usual places for a leak but
} } } can't find anything. We don't have a service contract right now so
} } } any help or ideas on why we are going through so much gas would be a
} } } big help.
} } }
} } } Thanks
} } }
} } } Elvin
} } }
} } } Login Host: 68.19.196.245
} } } --------------------------------------------------------------------
}
}
}
} ==============================Original Headers==============================
} 20, 36 -- From wesaia-at-iastate.edu Tue May 26 09:20:57 2009
} 20, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
} 20, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEKsRQ002148
} 20, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:20:55 -0500
} 20, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
} 20, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4QEKrpT006037;
} 20, 36 -- Tue, 26 May 2009 09:20:53 -0500
} 20, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
} 20, 36 -- id 0c88_6bdcb654_4a00_11de_a285_001372578af6;
} 20, 36 -- Tue, 26 May 2009 09:20:53 -0500
} 20, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
} 20, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4QEKqN3016931;
} 20, 36 -- Tue, 26 May 2009 09:20:52 -0500
} 20, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 20, 36 -- Tue, 26 May 2009 09:20:52 -0500
} 20, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 20, 36 -- Content-class: urn:content-classes:message
} 20, 36 -- MIME-Version: 1.0
} 20, 36 -- Content-Type: text/plain;
} 20, 36 -- charset="iso-8859-1"
} 20, 36 -- Subject: RE: [Microscopy] Re: viaWWW: Nitrogen leak
} 20, 36 -- Date: Tue, 26 May 2009 09:22:07 -0500
} 20, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703E4A06B-at-maire.eng.iastate.edu}
} 20, 36 -- In-Reply-To: {200905260956.n4Q9u554028058-at-ns.microscopy.com}
} 20, 36 -- X-MS-Has-Attach:
} 20, 36 -- X-MS-TNEF-Correlator:
} 20, 36 -- Thread-Topic: [Microscopy] Re: viaWWW: Nitrogen leak
} 20, 36 -- Thread-Index: Acnd6DD2Rgqp93I5SXyQuSYC2FQUAQAI46fg
} 20, 36 -- References: {200905260956.n4Q9u554028058-at-ns.microscopy.com}
} 20, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 20, 36 -- To: {nizets2-at-yahoo.com} , {Microscopy-at-microscopy.com}
} 20, 36 -- X-OriginalArrivalTime: 26 May 2009 14:20:52.0231 (UTC) FILETIME=[2CADF970:01C9DE0D]
} 20, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.26.141105
} 20, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0'
} 20, 36 -- Content-Transfer-Encoding: 8bit
} 20, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEKsRQ002148
} ==============================End of - Headers==============================

==============================Original Headers==============================
11, 22 -- From dac-at-research.umass.edu Tue May 26 10:16:39 2009
11, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QFGdgc004220
11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 10:16:39 -0500
11, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
11, 22 -- (authenticated bits=0)
11, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4QFGcKg015172
11, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 11:16:39 -0400
11, 22 -- Message-ID: {4A1C07D6.3040802-at-research.umass.edu}
11, 22 -- Date: Tue, 26 May 2009 11:16:38 -0400
11, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
11, 22 -- Reply-To: dac-at-research.umass.edu
11, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16
11, 22 -- MIME-Version: 1.0
11, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
11, 22 -- Subject: Re: [Microscopy] viaWWW: Nitrogen leak (is this really a problem?)
11, 22 -- References: {200905261426.n4QEQqUw014299-at-ns.microscopy.com}
11, 22 -- In-Reply-To: {200905261426.n4QEQqUw014299-at-ns.microscopy.com}
11, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 22 -- Content-Transfer-Encoding: 7bit
11, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Tue, 26 May 2009 13:15:59 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nitrogen gas is lighter than the air and so will rather go up than
down. What tricks us into assuming the opposite is the condensed water
"steam" that appears when LN2 is being poured.

Now, I hope this was not a bait to see who's a smart^$$ ;)

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com}
} Date: May 26, 2009 5:56:17 AM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] Re: viaWWW: Nitrogen leak
} Reply-To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Hi!
}
} Independently of the main message of your mail, I think it is a
} mistake to calculate in 3D even though we are talking about gas here.
} This is because N2 will not fill the room, but will fall on the
} floor. So I would be more realistic to calculate how high the level
} of N2 can be considering the surface of the actual room you are
} using. Then take into account the possibility that someone places
} his face near the ground for any reason, just to pick up (or search
} for) a pen which has fallen to the ground for example.
}
} Regards,
} Stephane
}
}
}
} ----- Original Message ----
} X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, May 20, 2009 3:04:22 PM
} Subject: [Microscopy] viaWWW: Nitrogen leak
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} John:
}
} Are you seriously stirring this up again? Didn't we just handle O2
} displacement via nitrogen boil off a few weeks ago?
}
} The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains
} only
} 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at
} 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a
} tiny room for a scope and all the N2 would have to be released
} rapidly enough to displace the O2 in the room (or reduce it from 22%
} to below 12% for this to become an issue let alone life threatening)
} without the N2 leaking out the doorway or any O2 leaking in. But in
} fact it sounds like ". . .going through nitrogen gas like there is no
} tomorrow." is probably days not minutes, and the scope room is a
} "normal room" and not a sealed environmental chamber.
}
} I have worked with compressed atmospheric air. No matter how dry
} it
} is an extremely corrosive material, degrading fittings, seals and
} regulator parts. I would suggest the long term costs and safety make
} working with compressed air a far worse choice than ppN2
}
}
} On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------ The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } Elgin;
} } My I suggest also that you have created a significant safety hazard
} } by
} } running your air table, and I presume your valving, on nitrogen. The
} } leak you now have (and everything develops leaks eventually) is
} } filling up your room with pure nitrogen and displacing the oxygen.
} } You
} } should be using only clean dry air for these items so that when it
} } leaks, the room fills up with.....air! Air that you can breath!
} } Nitrogen should only be used for venting the chamber.
} }
} }
} } Sent from my iPhone.
} } John Mardinly
} }
} } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
} }
} } }
} } }
} } }
} } } ---
} } } ---
} } } --------------------------------------------------------------------
} } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society
} } } of America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html --- ---
} } } --------------------------------------------------------------------
} } } --
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at
} } } http://www.microscopy.com/MLFormMail.html --- ---
} } } --------------------------------------------------------------------
} } } - Remember this posting is most likely not from a Subscriber, so
} } } when replying please copy both eawoodruff-at-live.com as well as
} } } the MIcroscopy Listserver --- ---
} } } --------------------------------------------------------------------
} } } -
} } }
} } } Email: eawoodruff-at-live.com
} } } Name: Elvin Woodruff III
} } }
} } } Organization: Tennessee State University
} } }
} } } Title-Subject: [Filtered] Nitrogen leak
} } }
} } } Question: Hi All,
} } }
} } } I have recently started working with a JEOL 6701F scanning electron
} } } microscopy. The scope is running just fine, but it is going through
} } } nitrogen gas like there is not tomorrow. The scope sits on an air
} } } table which is supplied by gas (could this be it?)and other areas
} } } such as the specimen load chamber is also filled with nitrogen gas.
} } } I have talked to a couple of other people about the gas loss but to
} } } no avail. I have checked all of the usual places for a leak but
} } } can't find anything. We don't have a service contract right now so
} } } any help or ideas on why we are going through so much gas would be a
} } } big help.
} } }
} } } Thanks
} } }
} } } Elvin
} } }
} } } Login Host: 68.19.196.245
} } } ---
} } } ---
} } } --------------------------------------------------------------------
} } } -
} } }
} } } ==============================Original
} } } Headers==============================
} } } 9, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:41:46 2009
} } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } } [206.69.208.22
} } } ])
} } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } } ESMTP id n48CfjvE003327 9, 11 -- for {microscopy-at-microscopy.com} ;
} } } Fri, 8 May 2009 07:41:46 -0500 9, 11 -- Mime-Version: 1.0 9, 11 --
} } } Message-Id: {p06240801c629d8f35379-at-[206.69.208.22]} 9, 11 -- Date:
} } } Fri, 8 May 2009 07:41:44 -0500 9, 11 -- To:
} } } microscopy-at-microscopy.com 9, 11 -- From: eawoodruff-at-live.com (by way
} } } of MicroscopyListserver) 9, 11 -- Subject: viaWWW: Nitrogen leak 9,
} } } 11 -- Content-Type: text/plain; charset="us-ascii" ;
} } } format="flowed" ==============================End of -
} } } Headers==============================
} }
} } ==============================Original
} } Headers============================== 5, 43 -- From
} } jmardinly-at-gmail.com Fri May 8 16:10:01 2009 5, 43 -- Received: from
} } wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.180]) 5,
} } 43
} } -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n48L9w3w012003 5, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 8
} } May
} } 2009 16:10:00 -0500 5, 43 -- Received: by wa-out-1112.google.com with



From: TindallR-at-missouri.edu
Date: Tue, 26 May 2009 13:49:37 -0500
Subject: [Microscopy] TEM: ortho-phthalaldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

Okay, here's a weird one. Has anyone heard of, or better yet, had any
experience with, using ortho-phthalaldehyde as a substitute for
glutaraldehyde in EM fixation? Cydex-OPA, in particular?

Seems one of our local hospitals is kicking about their researchers
fixing tissues for EM in glut-based fixatives and is pushing them hard
to find a substitute. My searches turn this up as a disinfectant, but
our director says it theoretically might work, based on the fact that
it's a dialdehyde.

Could this be the next great breakthrough in TEM?

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009
9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QInaYQ011348
9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/AQEJhmCIToJWgTUF
9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
9, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May 2009 13:49:36 -0500
9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 27 -- Tue, 26 May 2009 13:49:36 -0500
9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 27 -- Content-class: urn:content-classes:message
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Type: text/plain;
9, 27 -- charset="us-ascii"
9, 27 -- Subject: TEM: ortho-phthalaldehyde
9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500
9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu}
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde
9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw==
9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 27 -- To: {microscopy-at-microscopy.com}
9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC) FILETIME=[B73D51B0:01C9DE32]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QInaYQ011348
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 26 May 2009 14:14:09 -0500
Subject: [Microscopy] two more cents! - Nitrogen leak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Having once started a similar discussion let me put two cents in.

Inspection of the periodic chart indicate N2 should be lighter than air at
the same temperature and pressure. After all, air is just nitrogen
contaminated with heaver gasses like O2, CO2, hydrocarbons, H2O, noble
gases and only a really good analytical chemist knows what else. The point
is the LN2 as it evaporates or leaks out under pressure is colder than
anyone's room temperature, including that -40 degree room you're working
in. Colder is denser.

How fast it warms up and the effect of gas diffusion are topics beyond my
background.

Based on past discussions, I am now convinced that a large spill of liquid
N in a smallish room will ruin the vinyl floor tiles, cause serious injury
to you and cost you a pretty penny to repair/replace the dewar. It may
also end your life.

Leaking gases should be taken seriously.

stay safe......
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
8, 22 -- From frank_karl-at-lincolnelectric.com Tue May 26 14:14:09 2009
8, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QJE9Wq026305
8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 14:14:09 -0500
8, 22 -- In-Reply-To: {200905261827.n4QIR74K008389-at-ns.microscopy.com}
8, 22 -- Subject: two more cents! - Nitrogen leak
8, 22 -- To: vladislav_speransky-at-nih.gov, Microscopy-at-microscopy.com
8, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
8, 22 -- Message-ID: {OF379CE9F1.8FD458BC-ON852575C2.0067E866-852575C2.0069A284-at-lincolnelectric.com}
8, 22 -- Date: Tue, 26 May 2009 15:13:56 -0400
8, 22 -- From: Frank_Karl-at-lincolnelectric.com
8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM,
8, 22 -- CD-MIME complete at 05/26/2009 03:13:52 PM,
8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM,
8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM,
8, 22 -- Serialize complete at 05/26/2009 03:13:52 PM
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain;
8, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 26 May 2009 15:05:30 -0500
Subject: [Microscopy] Re: The Redox properties of minerals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On May 26, 2009, at 7:07 AM, nizets2-at-yahoo.com wrote:

} So I send this message in the bottle to the list. This is a call to
} anybody in the field who could help me devise a protocol, or simply
} send me a copy of the above paper.


Dear Stephane,
If you have the citation, I, or someone else from the list, could
attach a PDF of the paper to you.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Tue May 26 15:05:30 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QK5TlF009979
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 15:05:30 -0500
6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 7FE0A2E50B2A
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:05:29 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 84BD92E50E41
6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:05:28 -0700 (PDT)
6, 22 -- Message-Id: {A82D9DE8-5A08-4C9E-B595-07EFA815599D-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200905261407.n4QE7tp6024513-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
6, 22 -- Subject: Re: [Microscopy] The Redox properties of minerals
6, 22 -- Date: Tue, 26 May 2009 13:05:27 -0700
6, 22 -- References: {200905261407.n4QE7tp6024513-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Tue, 26 May 2009 18:53:09 -0500
Subject: [Microscopy] Looking for light microscope service in Florida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for someone in the Florida Tampa Bay area to work on,
clean, and repair an Olympus BX40 dark field microscope. Phone 813 220
4522, secret-at-mynikken.net

Please reply off list. Thank you.

Maurice Loeb


==============================Original Headers==============================
4, 18 -- From randerson20-at-tampabay.rr.com Tue May 26 18:53:09 2009
4, 18 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QNr9wT002726
4, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2009 18:53:09 -0500
4, 18 -- Received: from [127.0.0.1] (really [24.73.73.214])
4, 18 -- by hrndva-omta03.mail.rr.com with ESMTP
4, 18 -- id {20090526235308830.GJNL15928-at-hrndva-omta03.mail.rr.com}
4, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2009 23:53:08 +0000
4, 18 -- Message-ID: {4A1C80E0.30709-at-tampabay.rr.com}
4, 18 -- Date: Tue, 26 May 2009 19:53:04 -0400
4, 18 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
4, 18 -- Reply-To: secret-at-mynikken.net
4, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
4, 18 -- MIME-Version: 1.0
4, 18 -- To: Listserver {Microscopy-at-Microscopy.Com}
4, 18 -- Subject: Looking for light microscope service in Florida
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: shea.miller-at-agr.gc.ca
Date: Tue, 26 May 2009 19:14:44 -0500
Subject: [Microscopy] viaWWW: Light microscopy: localization of peroxidase in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both shea.miller-at-agr.gc.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: shea.miller-at-agr.gc.ca
Name: Shea Miller

Organization: Agriculture & AgriFood Canada

Title-Subject: [Filtered] Light microscopy: localization of
peroxidase in plants

Question: Hello all;
I have been trying to localize peroxidase in soybean seed coats,
and am wondering if anyone has a favourite protocol. The one I have
been using (chloronaphthol reagent) is a bit hit and miss, and I am
open to trying something new.

thanks in advance
shea

Login Host: 192.197.71.189
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue May 26 19:14:43 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R0EfTB017709
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 19:14:42 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c642365f91af-at-[206.69.208.22]}
7, 11 -- Date: Tue, 26 May 2009 19:14:41 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: shea.miller-at-agr.gc.ca (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Light microscopy: localization of peroxidase in plants
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Tue, 26 May 2009 23:31:26 -0500
Subject: [Microscopy] Fwd: TEM: ortho-phthalaldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I looked up the structure for this Cydex-OPA. Does not really look
like a good cross-linker. It is a benzene ring with two aldehyde
groups attached right next to each other. Quite different from that of
GA, which is a linear (flexible!) chain with an aldehyde group on each
end. Cydex-OPA molecule is bigger and less flexible. Organic Chemistry
was taken long time ago and never really needed, so I can't be fully
trusted here, but it appears that all bonds in Cydex-OPA can be
shared, stabilizing the whole structure - resonance, they call it in
English. Like in phenol. Also, the aldehyde groups are too close
together, another possible problem. All this considered, such molecule
will want to cross-link much less than GA - this is not even
considering how well it penetrates the structure.

It is a disinfectant? Sure, but so is phenol, which happens to be
quite similar in structure. Looking for the structural formula, I also
saw tons of disinfection references (and no cross-linking ones). Well,
from the point of view of whoever is pushing for that, going Green is
big right now, them bureaucrats they have all these "initiatives",
"roadmaps", you name it. If someone manages to eradicate GA from a
hospital, they'd score pretty big on that BS scale, I imagine.

Why doesn't somebody simply get a little of that stuff and tries fix a
tissue with it? I gave reasons above not too waste time on that, but a
few pictures will be a far stronger argument.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
} Date: May 26, 2009 9:03:22 PM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] TEM: ortho-phthalaldehyde
} Reply-To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Collective,
}
} Okay, here's a weird one. Has anyone heard of, or better yet, had any
} experience with, using ortho-phthalaldehyde as a substitute for
} glutaraldehyde in EM fixation? Cydex-OPA, in particular?
}
} Seems one of our local hospitals is kicking about their researchers
} fixing tissues for EM in glut-based fixatives and is pushing them hard
} to find a substitute. My searches turn this up as a disinfectant, but
} our director says it theoretically might work, based on the fact that
} it's a dialdehyde.
}
} Could this be the next great breakthrough in TEM?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original
} Headers==============================
} 9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009
} 9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
} (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n4QInaYQ011348
} 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36
} -0500
} 9, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/
} AQEJhmCIToJWgTUF
} 9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} ([209.106.229.41])
} 9, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May
} 2009 13:49:36 -0500
} 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft
} SMTPSVC(6.0.3790.3959);
} 9, 27 -- Tue, 26 May 2009 13:49:36 -0500
} 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 9, 27 -- Content-class: urn:content-classes:message
} 9, 27 -- MIME-Version: 1.0
} 9, 27 -- Content-Type: text/plain;
} 9, 27 -- charset="us-ascii"
} 9, 27 -- Subject: TEM: ortho-phthalaldehyde
} 9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500
} 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu
} }
} 9, 27 -- X-MS-Has-Attach:
} 9, 27 -- X-MS-TNEF-Correlator:
} 9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde
} 9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw==
} 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 9, 27 -- To: {microscopy-at-microscopy.com}
} 9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC)
} FILETIME=[B73D51B0:01C9DE32]
} 9, 27 -- Content-Transfer-Encoding: 8bit
} 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4QInaYQ011348
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
8, 23 -- From vladislav_speransky-at-nih.gov Tue May 26 23:31:26 2009
8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R4VQWV012789
8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 23:31:26 -0500
8, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id B4D183463BE
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:25 -0400 (EDT)
8, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
8, 23 -- by compute2.internal (MEProxy); Wed, 27 May 2009 00:31:25 -0400
8, 23 -- X-Sasl-enc: 0MIB3HGArrmbARYulZUbPptk8oXL0KbhZsh08gaeYQuP 1243398685
8, 23 -- Received: from [192.168.1.5] (pool-173-79-181-192.washdc.fios.verizon.net [173.79.181.192])
8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id B55A2463C6
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:24 -0400 (EDT)
8, 23 -- Message-Id: {8BAEDE10-0513-4244-BC16-6AA07CD2C1B9-at-nih.gov}
8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
8, 23 -- Subject: Fwd: [Microscopy] TEM: ortho-phthalaldehyde
8, 23 -- Date: Wed, 27 May 2009 00:31:22 -0400
8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2563D18B-at-NIHMLBX02.nih.gov}
8, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Wed, 27 May 2009 01:51:56 -0500
Subject: [Microscopy] Re: LM: localization of peroxidase in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,
hello all,

Dear Shea,
Despite I am not quite confident about similarity of peroxidase activity (-ies) in plants compared with animal tissues, I would like to point you to trying 3'3'-DAB (diamino-benzidine) as a medium for localization of peroxidase.

There is a whole issue of Histochem Cell Biol (2009) 131 No 4 dealing more/less with peroxisomal issues...
==} http://springerlink.com/content/x06832162703/

The Editorial was written by:
H. Dariush Fahimi
Peroxisomes: 40 years of histochemical staining, personal reminiscences

Histochem Cell Biol (2009) 131:437-440, DOI 10.1007/s00418-009-0562-8
Abstract: The historical circumstances that led to the discovery of the 3,3-diamino-benzidine (DAB) method for
staining of peroxisomes 40 years ago are reviewed. In the course of studies on the uptake and absorption of horse radish peroxidase in mammalian liver, in sections incubated for detection of peroxidase activity in DAB, it was noted that peroxisomes also stained positively for peroxidase activity. Subsequently, it was revealed that the peroxidatic activity of catalase, which is abundantly present in peroxisomes, is responsible for that staining. This notion was confirmed in quantitative biochemical studies with crystalline beef liver catalase and in tracer studies using catalase as an ultrastructural tracer. The application of the DAB method led to the discovery of peroxisomes as a ubiquitous eukaryotic cell organelle, attracting great interest in their investigation in biomedical research.

Perhaps an entry,

Good luck and best wishes

Wolfgang MUSS
EM-Lab, Pathology, SALK-PMU (Gen.Hosp.)
SALZBURG-Austria







} -----Ursprüngliche Nachricht-----
} Von: shea.miller-at-agr.gc.ca [mailto:shea.miller-at-agr.gc.ca]
} Gesendet: Mittwoch, 27. Mai 2009 02:17
} An: Muß Wolfgang
} Betreff: [Microscopy] LM: localization of peroxidase in plants
}
}
} --------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} --------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both shea.miller-at-agr.gc.ca as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} Email: shea.miller-at-agr.gc.ca
} Name: Shea Miller
} Organization: Agriculture & AgriFood Canada
} Title-Subject: Light
} microscopy: localization of peroxidase in plants
} Question:
}
} Hello all;
} I have been trying to localize peroxidase in soybean seed
} coats, and am wondering if anyone has a favourite protocol.
} The one I have been using (chloronaphthol reagent) is a bit
} hit and miss, and I am open to trying something new.
}
} thanks in advance
} shea
}
} Login Host: 192.197.71.189

} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Tue May 26 19:14:43 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R0EfTB017709
} 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 19:14:42 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240800c642365f91af-at-[206.69.208.22]}
} 7, 11 -- Date: Tue, 26 May 2009 19:14:41 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: shea.miller-at-agr.gc.ca (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Light microscopy: localization of peroxidase in plants
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
16, 36 -- From W.Muss-at-salk.at Wed May 27 01:51:56 2009
16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R6ptq5019121
16, 36 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 01:51:56 -0500
16, 36 -- Received: from localhost (localhost [127.0.0.1])
16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 6805DC386F;
16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST)
16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
16, 36 -- Received: from hermes.salk.at ([127.0.0.1])
16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
16, 36 -- with ESMTP id NnUaKMTIuB24; Wed, 27 May 2009 08:51:54 +0200 (CEST)
16, 36 -- Received: from n2rz123.lksdom21.lks.local (n2rz123.lksdom21.lks.local [192.168.101.123])
16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 00F04C386E;
16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST)
16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
16, 36 -- Wed, 27 May 2009 08:51:53 +0200
16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 36 -- Content-class: urn:content-classes:message
16, 36 -- MIME-Version: 1.0
16, 36 -- Content-Type: text/plain;
16, 36 -- charset="iso-8859-1"
16, 36 -- Subject: [Microscopy] Re: LM: localization of peroxidase in plants
16, 36 -- Date: Wed, 27 May 2009 08:51:53 +0200
16, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0A8E-at-N1RZ116.lksdom21.lks.local}
16, 36 -- In-Reply-To: {200905270017.n4R0HRhS023239-at-ns.microscopy.com}
16, 36 -- X-MS-Has-Attach:
16, 36 -- X-MS-TNEF-Correlator:
16, 36 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in plants
16, 36 -- Thread-Index: AcneYIpjXDYR3Wv0Q7ugkr2wHDdWZwANe1Lw
16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
16, 36 -- To: {shea.miller-at-agr.gc.ca}
16, 36 -- Cc: {microscopy-at-microscopy.com}
16, 36 -- X-OriginalArrivalTime: 27 May 2009 06:51:53.0972 (UTC) FILETIME=[9EA39B40:01C9DE97]
16, 36 -- X-Scanned-By: SALK-Content-Filter
16, 36 -- Content-Transfer-Encoding: 8bit
16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4R6ptq5019121
==============================End of - Headers==============================




From: malcolm.haswell-at-sunderland.ac.uk
Date: Tuesday, May 26, 2009 4:24 pm
Subject: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dale

I'm sorry but there are too many "mosts" and "probablys" in your
argument and to even say that oxygen content is approaching death zone
levels makes the basic assumption that any victim is a fit
acclimatized mountain climber to be relatively safe.

Risk assessment must look at the worst scenario anyway and assume that
someone may enter the room after the noisy leakage event occurred.
They would have no warning and would almost certainly be unaware of
what was happening to them. They might be the cleaner or an
inexperienced student and be in the room alone before/after anybody
else is in the building.

People do die in workplace/lab situations from oxygen depletion and
there is always the risk that rescue attempts can put more people at
risk.

So I would say do the risk assessment for your workplace looking at
the worst leakage and find out what would be considered a safe and
acceptable level of oxygen (certainly not as low as 12%). If your lab
falls below those requirements then do something such as better
ventilation, smaller source of gas or an oxygen depletion monitor with
an alarm.

Cheers

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: dac-at-research.umass.edu

==============================Original Headers==============================
12, 27 -- From malcolm.haswell-at-sunderland.ac.uk Wed May 27 03:49:32 2009
12, 27 -- Received: from max2.sunderland.ac.uk (max2.sunderland.ac.uk [157.228.98.76])
12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4R8nVeO018508
12, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 03:49:31 -0500
12, 27 -- Received: (qmail 1804 invoked from network); 27 May 2009 08:44:26 -0000
12, 27 -- Received: from localhost (127.0.0.1)
12, 27 -- by max2.sunderland.ac.uk with SMTP; 27 May 2009 08:44:26 -0000
12, 27 -- Received: (qmail 1742 invoked by uid 599); 27 May 2009 08:44:20 -0000
12, 27 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117)
12, 27 -- by max2.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 27 May 2009 09:44:20 +0100
12, 27 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed,
12, 27 -- 27 May 2009 09:44:21 +0100
12, 27 -- Date: Wed, 27 May 2009 09:44:21 +0100
12, 27 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
12, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a problem?)
12, 27 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
12, 27 -- Cc: dac-at-research.umass.edu
12, 27 -- Message-id: {241418243950.243950241418-at-sunderland.ac.uk}
12, 27 -- MIME-version: 1.0
12, 27 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004)
12, 27 -- Content-type: text/plain; charset=us-ascii
12, 27 -- Content-language: en
12, 27 -- Content-transfer-encoding: 7BIT
12, 27 -- Content-disposition: inline
12, 27 -- X-Accept-Language: en
12, 27 -- Priority: normal
12, 27 -- X-Virus-Scanned: by iCritical at max2.sunderland.ac.uk
==============================End of - Headers==============================




From: jberrim-at-nimr.mrc.ac.uk
Date: Wed, 27 May 2009 07:44:51 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak (is this really a problem?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jberrim-at-nimr.mrc.ac.uk as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: jberrim-at-nimr.mrc.ac.uk
Name: John Berriman

Title-Subject: [Filtered] Nitrogen leak (is this really a problem?)

Question: In situations where compressed gas has to be used in an
enclosed space why not use air rather than nitrogen?

Login Host: 81.129.165.50
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Wed May 27 07:44:50 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RCinQw011272
5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 07:44:50 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c642e62dc68f-at-[206.69.208.22]}
5, 11 -- Date: Wed, 27 May 2009 07:44:48 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: jberrim-at-nimr.mrc.ac.uk (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Nitrogen leak (is this really a problem?)
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wvrenter-at-sckcen.be
Date: Wed, 27 May 2009 07:55:00 -0500
Subject: [Microscopy] viaWWW: Covered TEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wvrenter-at-sckcen.be as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wvrenter-at-sckcen.be
Name: Wouter Van Renterghem

Organization: SCKïCEN

Title-Subject: [Filtered] Covered TEM specimen holder

Question: My institute has a JEOL 3010 TEM and I
want to investigate specimens which may not be
exposed to air. The problem is not that I need
to protect my specimen from air, but that I need
to guarantee that at all times my specimen will
not contaminate the environment. I can mount the
specimen on a TEM holder in a glove box, but I am
looking for a system to cover the specimen during
transport and mounting in the microscope.

Does there exist a TEM holder in which the
specimen is entirely encapsulated? Or is there
some kind of cask which can be placed aroud the
specimen tip and that can be connected to the
microscope?

Has somebody worked with specimens that could not
be exposed to air and can you tell how exposure
was avoided?

Thank you
Wouter Van Renterghem

Login Host: 193.190.187.220
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Wed May 27 07:54:59 2009
10, 13 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RCstSe018185
10, 13 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 07:54:57 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240804c642e88e5582-at-[206.69.208.22]}
10, 13 -- Date: Wed, 27 May 2009 07:54:54 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: wvrenter-at-sckcen.be (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: Covered TEM specimen holder
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4RCstSe018185
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 27 May 2009 08:27:52 -0500
Subject: [Microscopy] Fwd: TEM: ortho-phthalaldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am also skeptic. The presence of 2 aldhehyde groups is not sufficient to consider a molecule to be a good fixative.
What about the penetration? The speed of penetration? The solubility? The compatibility with biological buffers, with biological material, with stains, with processing, with resins?
What about the stability/reversability of the reactions? What about the pH and temperature-dependence of the reaction?
Theory is nice, but we still have found no alternative to practical testing for definitive assessment.
Please let us know how it worked :-).

On a side note, a benzene ring doesn't look particularly healthy in my mind of biologist. Is this product really so safe/so much safer than Glutar?

Regards,

Stephane



----- Original Message ----
X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov}
To: nizets2-at-yahoo.com
Sent: Wednesday, May 27, 2009 6:35:55 AM

I looked up the structure for this Cydex-OPA. Does not really look 
like a good cross-linker. It is a benzene ring with two aldehyde 
groups attached right next to each other. Quite different from that of 
GA, which is a linear (flexible!) chain with an aldehyde group on each 
end. Cydex-OPA molecule is bigger and less flexible. Organic Chemistry 
was taken long time ago and never really needed, so I can't be fully 
trusted here, but it appears that all bonds in Cydex-OPA can be 
shared, stabilizing the whole structure - resonance, they call it in 
English. Like in phenol. Also, the aldehyde groups are too close 
together, another possible problem. All this considered, such molecule 
will want to cross-link much less than GA - this is not even 
considering how well it penetrates the structure.

It is a disinfectant? Sure, but so is phenol, which happens to be 
quite similar in structure. Looking for the structural formula, I also 
saw tons of disinfection references (and no cross-linking ones). Well, 
from the point of view of whoever is pushing for that, going Green is 
big right now, them bureaucrats they have all these "initiatives", 
"roadmaps", you name it. If someone manages to eradicate GA from a 
hospital, they'd score pretty big on that BS scale, I imagine.

Why doesn't somebody simply get a little of that stuff and tries fix a 
tissue with it? I gave reasons above not too waste time on that, but a 
few pictures will be a far stronger argument.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do 
not represent the NIH. On the good side, this message is not 
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
} Date: May 26, 2009 9:03:22 PM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] TEM: ortho-phthalaldehyde
} Reply-To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of 
} America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Collective,
}
} Okay, here's a weird one.  Has anyone heard of, or better yet, had any
} experience with, using ortho-phthalaldehyde as a substitute for
} glutaraldehyde in EM fixation?  Cydex-OPA, in particular?
}
} Seems one of our local hospitals is kicking about their researchers
} fixing tissues for EM in glut-based fixatives and is pushing them hard
} to find a substitute.  My searches turn this up as a disinfectant, but
} our director says it theoretically might work, based on the fact that
} it's a dialdehyde.
}
} Could this be the next great breakthrough in TEM?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway:  http://www.sofn.com
}
}
}
}
} ==============================Original 
} Headers==============================
} 9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009
} 9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu 
} (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 9, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP 
} id n4QInaYQ011348
} 9, 27 --     for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36 
} -0500
} 9, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/
} AQEJhmCIToJWgTUF
} 9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) 
} ([209.106.229.41])
} 9, 27 --  by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May 
} 2009 13:49:36 -0500
} 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu 
} ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft 
} SMTPSVC(6.0.3790.3959);
} 9, 27 --     Tue, 26 May 2009 13:49:36 -0500
} 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 9, 27 -- Content-class: urn:content-classes:message
} 9, 27 -- MIME-Version: 1.0
} 9, 27 -- Content-Type: text/plain;
} 9, 27 --     charset="us-ascii"
} 9, 27 -- Subject: TEM: ortho-phthalaldehyde
} 9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500
} 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu
} }
} 9, 27 -- X-MS-Has-Attach:
} 9, 27 -- X-MS-TNEF-Correlator:
} 9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde
} 9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw==
} 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 9, 27 -- To: {microscopy-at-microscopy.com}
} 9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC) 
} FILETIME=[B73D51B0:01C9DE32]
} 9, 27 -- Content-Transfer-Encoding: 8bit
} 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by 
} ns.microscopy.com id n4QInaYQ011348
} ==============================End of - 
} Headers==============================


==============================Original Headers==============================
8, 23 -- From vladislav_speransky-at-nih.gov Tue May 26 23:31:26 2009
8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
8, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R4VQWV012789
8, 23 --     for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 23:31:26 -0500
8, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
8, 23 --     by out1.messagingengine.com (Postfix) with ESMTP id B4D183463BE
8, 23 --     for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:25 -0400 (EDT)
8, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
8, 23 --  by compute2.internal (MEProxy); Wed, 27 May 2009 00:31:25 -0400
8, 23 -- X-Sasl-enc: 0MIB3HGArrmbARYulZUbPptk8oXL0KbhZsh08gaeYQuP 1243398685
8, 23 -- Received: from [192.168.1.5] (pool-173-79-181-192.washdc.fios.verizon.net [173.79.181.192])
8, 23 --     by mail.messagingengine.com (Postfix) with ESMTPA id B55A2463C6
8, 23 --     for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:24 -0400 (EDT)
8, 23 -- Message-Id: {8BAEDE10-0513-4244-BC16-6AA07CD2C1B9-at-nih.gov}
8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
8, 23 -- Subject: Fwd: [Microscopy] TEM: ortho-phthalaldehyde
8, 23 -- Date: Wed, 27 May 2009 00:31:22 -0400
8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2563D18B-at-NIHMLBX02.nih.gov}
8, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================






==============================Original Headers==============================
25, 24 -- From nizets2-at-yahoo.com Wed May 27 08:27:51 2009
25, 24 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239])
25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4RDRoAb009077
25, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 08:27:50 -0500
25, 24 -- Received: (qmail 30902 invoked by uid 60001); 27 May 2009 13:27:48 -0000
25, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243430868; bh=AOjj9BscDZf0NTJUmfx588AejiwIakjCKk1Niam7N6c=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=BB3qUovinH1kAfIidCTaNq73RJxq8y4yQZ0hdQSGt9v89zx6n6OAnz9PZA/VhLCYGYGgTXNdoxlKdrzRBlV5yNbg4o5RTJRfBJmZdUenV1Vj9bnvtEim/U+HFQ1zOCtQ7jZ2e3eicz5INst6+jHxGnQ4/KgC9g0Bknn7z0N+S/M=
25, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
25, 24 -- s=s1024; d=yahoo.com;
25, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
25, 24 -- b=W4SCkGfgao7bNXZI7tdV21vKwhJb1MbX1yvAsh4wpytMeRM5Yx7bWtzBtMNTNlZCwEDNmSwNH73vSSsylod71YukOfdgjpB1J6LL48236NBhjHZ5knF4dO+lPatCXonHIUO+xLEWceZ9zv10wDySoo9qbxft/8WD2jYqqvMrGtw=;
25, 24 -- Message-ID: {634488.30550.qm-at-web110816.mail.gq1.yahoo.com}
25, 24 -- X-YMail-OSG: PKh7EG4VM1nxITvl6yszt5uIXVqMBr8t8.sNpzSjgf.KwZG4oH2tsqWXD0O4ioTWzQvrpRjfpZl2zJwoo8BE9XbmoV4_cumFYyVHpaIa2KnM5LZD9aN_INyNM.IYEFbbwe4KH5d5ZW6k4n5Vwmpkaq.QLU37DjX24y_7g5tldrYfyeILuGb_qsKpGZfZGGQ42zXOaZRwFRLytteLoA9uS710jISjSpIJJ34s0LfxkvZ6XdH5.RkkDh7Lwx65tpCHMzeOHOnKlFCimcyoWZb0NMQ7DialMNgeNRYaueyGI7xCGvtfIED91u.Dl46ZOMX0IoXksFDYmXBJI4Itov9diyRP
25, 24 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Wed, 27 May 2009 06:27:48 PDT
25, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
25, 24 -- References: {200905270435.n4R4ZtEp019244-at-ns.microscopy.com}
25, 24 -- Date: Wed, 27 May 2009 06:27:48 -0700 (PDT)
25, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
25, 24 -- Subject: Re: [Microscopy] Fwd: TEM: ortho-phthalaldehyde
25, 24 -- To: microscopy-at-microscopy.com
25, 24 -- In-Reply-To: {200905270435.n4R4ZtEp019244-at-ns.microscopy.com}
25, 24 -- MIME-Version: 1.0
25, 24 -- Content-Type: text/plain; charset=iso-8859-1
25, 24 -- Content-Transfer-Encoding: 8bit
25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4RDRoAb009077
==============================End of - Headers==============================




From: nicholls-at-uic.edu
Date: Wed, 27 May 2009 09:06:42 -0500
Subject: [Microscopy] Re: viaWWW: Covered TEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wouter

We have been successful using a glove bag instead of a glove box and
filling it with Argon. The specimen is loaded into the holder in the glove
bag and then with the help of other researchers is offered up to the
microscope airlock in the bag with a slight positive pressure of Argon. The
bag is opened slightly to enclose the airlock and the holder loaded into
the airlock. Once the pumping switches over to high vacuum the glove bag
can be removed.

This has also been successfully used to load reduced catalyst specimens
into an XPS with no measurable oxidation.

Regards

Alan

At 07:55 AM 5/27/2009, you wrote:

} Email: wvrenter-at-sckcen.be
} Name: Wouter Van Renterghem
}
} Organization: SCKïCEN
}
} Title-Subject: [Filtered] Covered TEM specimen holder
}
} Question: My institute has a JEOL 3010 TEM and I
} want to investigate specimens which may not be
} exposed to air. The problem is not that I need
} to protect my specimen from air, but that I need
} to guarantee that at all times my specimen will
} not contaminate the environment. I can mount the
} specimen on a TEM holder in a glove box, but I am
} looking for a system to cover the specimen during
} transport and mounting in the microscope.
}
} Does there exist a TEM holder in which the
} specimen is entirely encapsulated? Or is there
} some kind of cask which can be placed aroud the
} specimen tip and that can be connected to the
} microscope?
}
} Has somebody worked with specimens that could not
} be exposed to air and can you tell how exposure
} was avoided?
}
} Thank you
} Wouter Van Renterghem
}
} Login Host: 193.190.187.220
} ---------------------------------------------------------------------------
}
}

Alan W Nicholls, PhD
Interim Associate Director - RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu


==============================Original Headers==============================
11, 20 -- From nicholls-at-uic.edu Wed May 27 09:06:42 2009
11, 20 -- Received: from mail-3.priv.cc.uic.edu (mail-3.cc.uic.edu [128.248.155.42])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RE6fA2025263
11, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 09:06:41 -0500
11, 20 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241])
11, 20 -- (authenticated bits=0)
11, 20 -- by mail-3.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id n4RE6cQL011427
11, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
11, 20 -- Wed, 27 May 2009 09:06:39 -0500
11, 20 -- Message-Id: {6.2.1.2.2.20090527085929.031cb430-at-mail.uic.edu}
11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2
11, 20 -- Date: Wed, 27 May 2009 09:06:35 -0500
11, 20 -- To: wvrenter-at-sckcen.be, Microscopy-at-microscopy.com
11, 20 -- From: Alan Nicholls {nicholls-at-uic.edu}
11, 20 -- Subject: Re: [Microscopy] viaWWW: Covered TEM specimen holder
11, 20 -- In-Reply-To: {200905271255.n4RCtcMi019848-at-ns.microscopy.com}
11, 20 -- References: {200905271255.n4RCtcMi019848-at-ns.microscopy.com}
11, 20 -- Mime-Version: 1.0
11, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
11, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Wed, 27 May 2009 10:04:56 -0500
Subject: [Microscopy] viaWWW: Nitrogen leak (is this really a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dale,

Let's assume a six foot wide fume hood with a bypass grill 'overhead', has
a face velocity of 100 feet per minute, and a closed sash opening area of
12 square feet. That means that the cubic feet per minute of air flow is
1200 CFM. Let's use a 12 x 12 x 10 foot room that has a HVAC system of air
turnover. That room has a volume of 1440 cubic feet. So a hood outside
this room might be supplied by as many as three inlet air vents. So the
air turnover in the leaking nitrogen room could be as low as about 480 CFM.
So after three minutes, the volume of normal air forced into the room is
equal to the volume of the room.

It takes five volumes of a gas to flush out all the collected gas in a gas
collection bulb. Let's assume that is the correct volume needed for a lab
of volume 1440 CF. Five volumes circulated without a leak is 7200 CF.
7200/1200 = 6 minutes (for five volumes of room air). Now let's displace
some of that air with nitrogen. Let's lower the O2 level to about 16% from
20%. The dilution formula is C1*V1 = C2*V2. (20%)(V1) = (16%)(7200). V1
= 5760 CF of air. 7200 - 5760 = 1440 CF of extra nitrogen is needed in six
minutes. That's means 240 CFM of nitrogen leakage is needed to create a
16% O2 atmosphere from adding pure nitrogen to the HVAC air containing 20%
O2 every minute.

240 CFM is a leakage rate of 6,796 liters of nitrogen per minute. That's a
huge nitrogen leak for a pinhole in an air table bladder or a Philips EM
column bladder. Considering your N2 cylinder has only 225 CF per tank, one
would have to empty more than one cylinder per minute, probably without a
regulator! The nitrogen will be compressed in the bladder but once it
leaks out, it is 240 CFM.

That leakage rate might be a fairly noisy gas leak for an air table, IMO.

"(is this really a problem?)" Not in my opinion.

Paul


At 10:17 AM 5/26/09 -0500, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
9, 27 -- From beaurega-at-westol.com Wed May 27 10:04:56 2009
9, 27 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RF4tcE009484
9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 10:04:55 -0500
9, 27 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
9, 27 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n4RF4if3025800
9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 11:04:46 -0400
9, 27 -- Received: (qmail 20516 invoked by uid 89); 27 May 2009 13:17:59 -0000
9, 27 -- Received: from pitts-69-72-117-93.dynamic-dialup.coretel.net (HELO running) (69.72.117.93)
9, 27 -- by mail.winbeam.com with SMTP; 27 May 2009 13:17:59 -0000
9, 27 -- Message-Id: {3.0.6.32.20090527091735.0085f100-at-pop3.norton.antivirus}
9, 27 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus
9, 27 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
9, 27 -- Date: Wed, 27 May 2009 09:17:35 -0400
9, 27 -- To: microscopy-at-microscopy.com
9, 27 -- From: Beaurega {beaurega-at-westol.com}
9, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a
9, 27 -- problem?)
9, 27 -- Mime-Version: 1.0
9, 27 -- Content-Type: text/plain; charset="us-ascii"
9, 27 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
9, 27 -- X-Winbeam-MailScanner-ID: n4RF4if3025800
9, 27 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
9, 27 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted),
9, 27 -- SpamAssassin (not cached, score=-1.759, required 4,
9, 27 -- autolearn=not spam, AWL 0.16, BAYES_00 -2.00, TW_FT 0.08)
9, 27 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Wed, 27 May 2009 11:17:40 -0500
Subject: [Microscopy] viaWWW: Covered TEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wouter,

We have the SampleSaver(TM) Storage Container that will store and transport
samples in a slightly pressurized inert atmosphere. Here is a link to
information on the units, http://www.southbaytech.com/pdfs/SS1.pdf. We have
different racks including one that will store TEM grids. This unit was
designed for preventing oxidation of samples, but it would also be a
solution for you. They can be used quite easily with a glove bag or a glove
box. When you do use a bag or box, you do not need to purge the container
as they are normally used. I saw that Alan Nicholls already discussed using
a glove bag to load the TEM holder and into the TEM airlock. I would use N2
instead of Ar because of the expense and the possibility of using house N2
if you have it.

You might also look into some of the cryogenic holders. They can load the
sample under a N2 atmosphere and some have a little slide cover that
protects the sample during transport from the cyro-workstation to the
microscope. I know that Gatan has a system like that. Here is a link to
such a holder on their site:
http://gatan.com/products/specimen_holders/products/CT3500TR_CryoTransferTil
tRotate.php.




Disclaimer: SBT manufactures and sells the SampleSaver(TM) Storage
Container.

-Scott




Scott D. Walck, Ph.D.
Technical Director

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

t: +1-949-492-2600 
f: +1-949-492-1499
e: swalck-at-southbaytech.com
 
www.SouthBayTech.com



-----Original Message-----
X-from: wvrenter-at-sckcen.be [mailto:wvrenter-at-sckcen.be]
Sent: Wednesday, May 27, 2009 6:06 AM
To: swalck-at-southbaytech.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wvrenter-at-sckcen.be as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wvrenter-at-sckcen.be
Name: Wouter Van Renterghem

Organization: SCKïCEN

Title-Subject: [Filtered] Covered TEM specimen holder

Question: My institute has a JEOL 3010 TEM and I
want to investigate specimens which may not be
exposed to air. The problem is not that I need
to protect my specimen from air, but that I need
to guarantee that at all times my specimen will
not contaminate the environment. I can mount the
specimen on a TEM holder in a glove box, but I am
looking for a system to cover the specimen during
transport and mounting in the microscope.

Does there exist a TEM holder in which the
specimen is entirely encapsulated? Or is there
some kind of cask which can be placed aroud the
specimen tip and that can be connected to the
microscope?

Has somebody worked with specimens that could not
be exposed to air and can you tell how exposure
was avoided?

Thank you
Wouter Van Renterghem

Login Host: 193.190.187.220
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Wed May 27 07:54:59 2009
10, 13 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov
[130.202.238.72])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n4RCstSe018185
10, 13 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 07:54:57
-0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240804c642e88e5582-at-[206.69.208.22]}
10, 13 -- Date: Wed, 27 May 2009 07:54:54 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: wvrenter-at-sckcen.be (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: Covered TEM specimen holder
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n4RCstSe018185
==============================End of - Headers==============================
Internal Virus Database is out of date.
Checked by AVG - http://www.avg.com
Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008
5:29 PM




==============================Original Headers==============================
34, 29 -- From swalck-at-southbaytech.com Wed May 27 11:17:39 2009
34, 29 -- Received: from n25.bullet.mail.mud.yahoo.com (n25.bullet.mail.mud.yahoo.com [68.142.206.220])
34, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4RGHdJ1027027
34, 29 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 11:17:39 -0500
34, 29 -- Received: from [209.191.108.96] by n25.bullet.mail.mud.yahoo.com with NNFMP; 27 May 2009 16:17:39 -0000
34, 29 -- Received: from [68.142.201.73] by t3.bullet.mud.yahoo.com with NNFMP; 27 May 2009 16:17:39 -0000
34, 29 -- Received: from [127.0.0.1] by omp425.mail.mud.yahoo.com with NNFMP; 27 May 2009 16:17:39 -0000
34, 29 -- X-Yahoo-Newman-Id: 109340.49186.bm-at-omp425.mail.mud.yahoo.com
34, 29 -- Received: (qmail 10949 invoked from network); 27 May 2009 16:17:38 -0000
34, 29 -- Received: from unknown (HELO dynamicbl8uno3) (swalck-at-99.154.21.201 with login)
34, 29 -- by smtp111.plus.mail.sp1.yahoo.com with SMTP; 27 May 2009 16:17:38 -0000
34, 29 -- X-YMail-OSG: ioT4_vgVM1nDgk8vIf1i01sYIbk1eYEa9ICb7gSxgkUSw1g79Lw1DyZobvjNHJlCqbfb2JR5zmQNYacX1861PqFLQ8ND6rfriC51Dl0vM8.ASmFtY6pDZjCIztWkrahXY49gL2AdhqXMdsrr.ylKb7L3KXuCigl16A3.X6VTIac3Ung..OrjVqmRmiuwphx3G6HZ71ADwibOt5vxkqivULjKDeAfYulbYAW.Y9aVHdJrw7N5C.uWi8eOTSF9ACAbRveobKMXELmezG2AnI_9ITo94rFY_oqr06N3PO.e2iR0zC.BmSU-
34, 29 -- X-Yahoo-Newman-Property: ymail-3
34, 29 -- From: "Dr. Scott Walck" {swalck-at-southbaytech.com}
34, 29 -- To: {Microscopy-at-microscopy.com}
34, 29 -- Cc: {wvrenter-at-sckcen.be} , "Gary Heineman" {gheineman-at-southbaytech.com}
34, 29 -- References: {200905271306.n4RD66Ik007335-at-ns.microscopy.com}
34, 29 -- In-Reply-To: {200905271306.n4RD66Ik007335-at-ns.microscopy.com}
34, 29 -- Subject: RE: [Microscopy] viaWWW: Covered TEM specimen holder
34, 29 -- Date: Wed, 27 May 2009 09:17:43 -0700
34, 29 -- Message-ID: {007e01c9dee6$aa345310$fe9cf930$-at-com}
34, 29 -- MIME-Version: 1.0
34, 29 -- Content-Type: text/plain;
34, 29 -- charset="iso-8859-1"
34, 29 -- X-Mailer: Microsoft Office Outlook 12.0
34, 29 -- Thread-Index: Acney+bguQYrOD/YSNuwuJVf2rbGPgAF7qtw
34, 29 -- Content-Language: en-us
34, 29 -- Content-Transfer-Encoding: 8bit
34, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4RGHdJ1027027
==============================End of - Headers==============================




From: astrohsc-at-aerotek.com
Date: Wed, 27 May 2009 14:28:05 -0500
Subject: [Microscopy] viaWWW: Immediate need for an SEM Operator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both astrohsc-at-aerotek.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: astrohsc-at-aerotek.com
Name: Andrew Strohschein

Organization: Aerotek Scientific

Title-Subject: [Filtered] Immediate need for an SEM Operator

Question: Aerotek Scientific is looking for an SEM Operator to work
on a project basis through the end of the year at a Minnesota based
medical device company.

Qualified candidates need to have experience with SEM-EDS. This
person will be performing material verification / material
composition, failure analysis on medical devices.

Interested candidates should send resumes to astrohsc-at-aerotek.com or
contact Andrew directly at 763-852-1349.

Login Host: 216.152.101.68
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed May 27 14:28:04 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJS3EC019065
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:28:04 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c64344b2ee52-at-[206.69.208.22]}
8, 11 -- Date: Wed, 27 May 2009 14:28:02 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: astrohsc-at-aerotek.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Immediate need for an SEM Operator
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hong_s-at-palmer.edu
Date: Wed, 27 May 2009 14:28:31 -0500
Subject: [Microscopy] viaWWW: reembedment of a semithin section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hong_s-at-palmer.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hong_s-at-palmer.edu
Name: Se Pyo Hong

Organization: Graduate Studies at Palmer College of Chiropractic

Title-Subject: [Filtered] reembedment of a semithin section

Question: Hi everyone;
I have 2-micron thick flat specimens (cross sectioned rat spinal
cord) embedded in epon with toluidine stain on a glass slide. I want
to have ultrathin sections of part of the specimen (Raxed lamina 1 -
3 area). Please give me suggestions or a procedure. Thank you..


Login Host: 198.102.161.2
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed May 27 14:28:30 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJSTj8019343
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:28:29 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c64344cbf431-at-[206.69.208.22]}
7, 11 -- Date: Wed, 27 May 2009 14:28:27 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: hong_s-at-palmer.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: reembedment of a semithin section
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Wed, 27 May 2009 14:50:18 -0500
Subject: [Microscopy] RE;Covered spec rod

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have designed specimen rods to protect specimens from the
atmosphere during transfer from a glove box to the microscope which
have performed successfully in both a JEOL 2010 TEM and a JEOL
HF-2200 aberration-corrected TEM. I believe the specimen rod for the 3010 is
very similar to these others, and so a specimen rod of this design
should work for you. Let me know if you want more information
--
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
1, 15 -- From bigelow-at-umich.edu Wed May 27 14:50:18 2009
1, 15 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160])
1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJoIhO015434
1, 15 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:50:18 -0500
1, 15 -- Received: FROM [141.212.131.153] (bigelow-imac-g5.engin.umich.edu [141.212.131.153])
1, 15 -- By skycaptain.mr.itd.umich.edu ID 4A1D9979.C4FA8.8883 ;
1, 15 -- Authuser bigelow;
1, 15 -- 27 May 2009 15:50:17 EDT
1, 15 -- Mime-Version: 1.0
1, 15 -- Message-Id: {p06240800c643495659e5-at-[141.212.131.153]}
1, 15 -- Date: Wed, 27 May 2009 15:50:16 -0400
1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 15 -- Subject: [Microscopy]RE;Covered spec rod
1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Wed, 27 May 2009 21:39:04 -0500
Subject: [Microscopy] Re: Re: LM: localization of peroxidase in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Shea,

You can certainly use DAB to detect ROX in plants, also a fluorescent probe,
H2DCFDA to detect various oxygen radicals - results of peroxidase activity,
I guess, and there are others to detect H2O2 as well - a number of new
probes have been synthesised lately.


cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 27/05/09 4:58 PM, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Good morning,
} hello all,
}
} Dear Shea,
} Despite I am not quite confident about similarity of peroxidase activity
} (-ies) in plants compared with animal tissues, I would like to point you to
} trying 3'3'-DAB (diamino-benzidine) as a medium for localization of
} peroxidase.
}
} There is a whole issue of Histochem Cell Biol (2009) 131 No 4 dealing
} more/less with peroxisomal issues...
} ==} http://springerlink.com/content/x06832162703/
}
} The Editorial was written by:
} H. Dariush Fahimi
} Peroxisomes: 40 years of histochemical staining, personal reminiscences
}
} Histochem Cell Biol (2009) 131:437-440, DOI 10.1007/s00418-009-0562-8
} Abstract: The historical circumstances that led to the discovery of the
} 3,3-diamino-benzidine (DAB) method for
} staining of peroxisomes 40 years ago are reviewed. In the course of studies on
} the uptake and absorption of horse radish peroxidase in mammalian liver, in
} sections incubated for detection of peroxidase activity in DAB, it was noted
} that peroxisomes also stained positively for peroxidase activity.
} Subsequently, it was revealed that the peroxidatic activity of catalase, which
} is abundantly present in peroxisomes, is responsible for that staining. This
} notion was confirmed in quantitative biochemical studies with crystalline beef
} liver catalase and in tracer studies using catalase as an ultrastructural
} tracer. The application of the DAB method led to the discovery of peroxisomes
} as a ubiquitous eukaryotic cell organelle, attracting great interest in their
} investigation in biomedical research.
}
} Perhaps an entry,
}
} Good luck and best wishes
}
} Wolfgang MUSS
} EM-Lab, Pathology, SALK-PMU (Gen.Hosp.)
} SALZBURG-Austria
}
}
}
}
}
}
}
} } -----Ursprüngliche Nachricht-----
} } Von: shea.miller-at-agr.gc.ca [mailto:shea.miller-at-agr.gc.ca]
} } Gesendet: Mittwoch, 27. Mai 2009 02:17
} } An: Muß Wolfgang
} } Betreff: [Microscopy] LM: localization of peroxidase in plants
} }
} }
} } --------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} } --------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber,
} } so when replying
} } please copy both shea.miller-at-agr.gc.ca as well as the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } Email: shea.miller-at-agr.gc.ca
} } Name: Shea Miller
} } Organization: Agriculture & AgriFood Canada
} } Title-Subject: Light
} } microscopy: localization of peroxidase in plants
} } Question:
} }
} } Hello all;
} } I have been trying to localize peroxidase in soybean seed
} } coats, and am wondering if anyone has a favourite protocol.
} } The one I have been using (chloronaphthol reagent) is a bit
} } hit and miss, and I am open to trying something new.
} }
} } thanks in advance
} } shea
} }
} } Login Host: 192.197.71.189
}
} } ==============================Original Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Tue May 26 19:14:43 2009
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n4R0EfTB017709
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 19:14:42 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240800c642365f91af-at-[206.69.208.22]}
} } 7, 11 -- Date: Tue, 26 May 2009 19:14:41 -0500
} } 7, 11 -- To: microscopy-at-microscopy.com
} } 7, 11 -- From: shea.miller-at-agr.gc.ca (by way of MicroscopyListserver)
} } 7, 11 -- Subject: viaWWW: Light microscopy: localization of peroxidase in
} } plants
} } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } ==============================End of - Headers==============================
} }
}
}
} ==============================Original Headers==============================
} 16, 36 -- From W.Muss-at-salk.at Wed May 27 01:51:56 2009
} 16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
} 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n4R6ptq5019121
} 16, 36 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 01:51:56 -0500
} 16, 36 -- Received: from localhost (localhost [127.0.0.1])
} 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 6805DC386F;
} 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST)
} 16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
} 16, 36 -- Received: from hermes.salk.at ([127.0.0.1])
} 16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port
} 10024)
} 16, 36 -- with ESMTP id NnUaKMTIuB24; Wed, 27 May 2009 08:51:54 +0200 (CEST)
} 16, 36 -- Received: from n2rz123.lksdom21.lks.local
} (n2rz123.lksdom21.lks.local [192.168.101.123])
} 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 00F04C386E;
} 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST)
} 16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by
} n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
} 16, 36 -- Wed, 27 May 2009 08:51:53 +0200
} 16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 16, 36 -- Content-class: urn:content-classes:message
} 16, 36 -- MIME-Version: 1.0
} 16, 36 -- Content-Type: text/plain;
} 16, 36 -- charset="iso-8859-1"
} 16, 36 -- Subject: [Microscopy] Re: LM: localization of peroxidase in plants
} 16, 36 -- Date: Wed, 27 May 2009 08:51:53 +0200
} 16, 36 -- Message-ID:
} {06B4ED29F824524E98E8AA5BB64070625D0A8E-at-N1RZ116.lksdom21.lks.local}
} 16, 36 -- In-Reply-To: {200905270017.n4R0HRhS023239-at-ns.microscopy.com}
} 16, 36 -- X-MS-Has-Attach:
} 16, 36 -- X-MS-TNEF-Correlator:
} 16, 36 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in
} plants
} 16, 36 -- Thread-Index: AcneYIpjXDYR3Wv0Q7ugkr2wHDdWZwANe1Lw
} 16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
} 16, 36 -- To: {shea.miller-at-agr.gc.ca}
} 16, 36 -- Cc: {microscopy-at-microscopy.com}
} 16, 36 -- X-OriginalArrivalTime: 27 May 2009 06:51:53.0972 (UTC)
} FILETIME=[9EA39B40:01C9DE97]
} 16, 36 -- X-Scanned-By: SALK-Content-Filter
} 16, 36 -- Content-Transfer-Encoding: 8bit
} 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4R6ptq5019121
} ==============================End of - Headers==============================




==============================Original Headers==============================
12, 45 -- From prvs=392b67a6d=Rosemary.White-at-csiro.au Wed May 27 21:39:04 2009
12, 45 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37])
12, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4S2d2w9011843
12, 45 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 21:39:03 -0500
12, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
12, 45 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
12, 45 -- s=email; t=1243478343; x=1275014343;
12, 45 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
12, 45 -- mime-version:content-transfer-encoding:content-id:
12, 45 -- content-description:resent-date:resent-from:resent-sender:
12, 45 -- resent-to:resent-cc:resent-message-id:in-reply-to:
12, 45 -- references:list-id:list-help:list-unsubscribe:
12, 45 -- list-subscribe:list-post:list-owner:list-archive;
12, 45 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
12, 45 -- |Subject:=20Re:=20[Microscopy]=20=20Re:=20LM:=20localizat
12, 45 -- ion=20of=20peroxidase=20in=20plants|Date:=20Thu,=2028=20M
12, 45 -- ay=202009=2012:38:59=20+1000|Message-ID:=20 {C6443663.6042
12, 45 -- %rosemary.white-at-csiro.au} |To:=20 {microscopy-at-microscopy.co
12, 45 -- m} |MIME-Version:=201.0|Content-Transfer-Encoding:=20quote
12, 45 -- d-printable|In-Reply-To:=20 {200905270658.n4R6wUC9029042-at-n
12, 45 -- s.microscopy.com} ;
12, 45 -- bh=kRwahgIhDs/fQvs54i2G8XaE70Ki7MAU3nuEBYgo7wI=;
12, 45 -- b=kX18N9i+Xm6q6XY/y5reNXaC38t19WLyPfFtgvN7aK9FOYxzpm5evFXI
12, 45 -- /vDX5+casKGD2XuNHb5qbuPteWGGKRClkBC/55kHPjVHF3Hp13uWPFoTN
12, 45 -- R/mBcV037I3jvUw;
12, 45 -- X-IronPort-AV: E=Sophos;i="4.41,262,1241359200";
12, 45 -- d="scan'208";a="27520030"
12, 45 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
12, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 28 May 2009 12:39:01 +1000
12, 45 -- Received: from [152.83.193.49] (152.83.193.49) by EXNSW-HTCA01.nexus.csiro.au
12, 45 -- (130.155.117.126) with Microsoft SMTP Server id 8.1.358.0; Thu, 28 May 2009
12, 45 -- 12:39:00 +1000
12, 45 -- User-Agent: Microsoft-Entourage/12.10.0.080409
12, 45 -- Date: Thu, 28 May 2009 12:38:59 +1000
12, 45 -- Subject: Re: [Microscopy] Re: LM: localization of peroxidase in plants
12, 45 -- From: Rosemary White {rosemary.white-at-csiro.au}
12, 45 -- To: {microscopy-at-microscopy.com}
12, 45 -- Message-ID: {C6443663.6042%rosemary.white-at-csiro.au}
12, 45 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in plants
12, 45 -- Thread-Index: AcnemI1XpPjTEhxkQJqtG4t3e1HaMgApOa7g
12, 45 -- In-Reply-To: {200905270658.n4R6wUC9029042-at-ns.microscopy.com}
12, 45 -- MIME-Version: 1.0
12, 45 -- Content-Type: text/plain; charset="ISO-8859-1"
12, 45 -- Content-Transfer-Encoding: 8bit
12, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4S2d2w9011843
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Thu, 28 May 2009 07:38:04 -0500
Subject: [Microscopy] viaWWW: TEM: Quietening turbo pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: Dave Mitchell

Organization: EM Unit, University of Sydney

Title-Subject: [Filtered] TEM: Quietening turbo pumps

Question: Dear All

I have a new dry pumped TEM. The vacuum and performance is to
specification and the machine is operating perfectly. The system is
backed by two incredibly noisy scroll pumps. I have built a
sound-proof enclosure for these and it has made a huge difference.
However, in the new found hush, the whine of the two turbo pumps is
noticeable. It is not excessive, it is just annoying (to me anyway).
The pumps are water cooled.

Question: Has anyone attempted to quieten such pumps by wrapping them
in sound absorbing material, and if so, did it make any difference?

Login Host: 129.78.220.7
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:02 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SCc1LJ008615
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 07:38:02 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c644361682e2-at-[206.69.208.22]}
8, 11 -- Date: Thu, 28 May 2009 07:38:01 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: TEM: Quietening turbo pumps
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: liina170-at-hot.ee
Date: Thu, 28 May 2009 07:38:54 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both liina170-at-hot.ee as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: liina170-at-hot.ee
Name: Liina

Organization: University of Tartu

Title-Subject: [Filtered] SEM and TEM

Question: What would be the main advantages and disadvantages of a
SEM that operates upside down i.e. has e-gun at the bottom pointing
upwards. What about horizontal design? The same questions about TEM.


Login Host: 213.184.38.91
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SCcqfd009244
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 07:38:53 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: liina170-at-hot.ee (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: SEM and TEM Column Orientation
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 28 May 2009 07:58:56 -0500
Subject: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For SEM it would be very inconvenient to mount specimens upside down, in
addition all the dirt and dust will fall down in the direction of an
electron gun, contaminating it. TEM column is too tall to mount it
upside down, and fluorescence screen should be transparent. Horizontal
positioning will take much more space than vertical one, and again will
introduce inconvenience in mounting SEM specimens and observation of TEM
screen.

Physics allows any positioning of a column in space; ergonomics speaks
for traditional one, vertical with a gun on the top.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} Sent: Thursday, May 28, 2009 7:40 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://www.microscopy.com/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both liina170-at-hot.ee as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: liina170-at-hot.ee
} Name: Liina
}
} Organization: University of Tartu
}
} Title-Subject: [Filtered] SEM and TEM
}
} Question: What would be the main advantages and disadvantages
} of a SEM that operates upside down i.e. has e-gun at the
} bottom pointing upwards. What about horizontal design? The
} same questions about TEM.
}
}
} Login Host: 213.184.38.91
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n4SCcqfd009244
} 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} 2009 07:38:53 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by
} way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM
} and TEM Column Orientation 7, 11 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009
8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.167])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SCwsIq007428
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 07:58:55 -0500
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Thu, 28 May 2009 07:58:53 -0500
8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="us-ascii"
8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) FILETIME=[0D6DBFE0:01C9DF94]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SCwsIq007428
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Thu, 28 May 2009 08:19:02 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Not to mention that on an inverted column the electrons have to
overcome gravity to make their way from the gun to the sample! {...grin...}

Henk

At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:


} For SEM it would be very inconvenient to mount specimens upside down, in
} addition all the dirt and dust will fall down in the direction of an
} electron gun, contaminating it. TEM column is too tall to mount it
} upside down, and fluorescence screen should be transparent. Horizontal
} positioning will take much more space than vertical one, and again will
} introduce inconvenience in mounting SEM specimens and observation of TEM
} screen.
}
} Physics allows any positioning of a column in space; ergonomics speaks
} for traditional one, vertical with a gun on the top.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} } Sent: Thursday, May 28, 2009 7:40 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} } This Question/Comment was submitted to the Microscopy
} } Listserver using the WWW based Form at
} } http://www.microscopy.com/MLFormMail.html
} } --------------------------------------------------------------
} } -------------
} } Remember this posting is most likely not from a Subscriber,
} } so when replying
} } please copy both liina170-at-hot.ee as well as the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } -------------
} }
} } Email: liina170-at-hot.ee
} } Name: Liina
} }
} } Organization: University of Tartu
} }
} } Title-Subject: [Filtered] SEM and TEM
} }
} } Question: What would be the main advantages and disadvantages
} } of a SEM that operates upside down i.e. has e-gun at the
} } bottom pointing upwards. What about horizontal design? The
} } same questions about TEM.
} }
} }
} } Login Host: 213.184.38.91
} } --------------------------------------------------------------
} } -------------
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n4SCcqfd009244
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} } 2009 07:38:53 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by
} } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM
} } and TEM Column Orientation 7, 11 -- Content-Type: text/plain;
} } charset="us-ascii" ; format="flowed"
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009
} 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu
} (smtp.exchange.umkc.edu [134.193.143.167])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4SCwsIq007428
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
} 07:58:55 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Thu, 28 May 2009 07:58:53 -0500
} 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500
} 8, 25 -- Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
} 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC)
} FILETIME=[0D6DBFE0:01C9DF94]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4SCwsIq007428
} ==============================End of - Headers==============================

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009
7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDJ0tP023075
7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:19:01 -0500
7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
7, 26 -- id {01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
7, 26 -- Thu, 28 May 2009 09:18:58 -0400 (EDT)
7, 26 -- Received: from HOC1.ecr6.ohio-state.edu
7, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
7, 26 -- (PMDF V6.3-x18 #31556)
7, 26 -- with ESMTPA id {01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu,
7, 26 -- 28 May 2009 09:18:58 -0400 (EDT)
7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400
7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
7, 26 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation
7, 26 -- In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com}
7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
7, 26 -- To: DusevichV-at-umkc.edu
7, 26 -- Cc: Microscopy-at-microscopy.com
7, 26 -- Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu}
7, 26 -- MIME-version: 1.0
7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
7, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
7, 26 -- References: {200905281300.n4SD0xOu009804-at-ns.microscopy.com}
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Thu, 28 May 2009 08:24:47 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen pictures of a horizontal TEM from the early days.

Dave


-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: 28 May 2009 14:23
To: David Patton

Not to mention that on an inverted column the electrons have to overcome
gravity to make their way from the gun to the sample! {...grin...}

Henk

At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:


} For SEM it would be very inconvenient to mount specimens upside down,
} in addition all the dirt and dust will fall down in the direction of an

} electron gun, contaminating it. TEM column is too tall to mount it
} upside down, and fluorescence screen should be transparent. Horizontal
} positioning will take much more space than vertical one, and again will

} introduce inconvenience in mounting SEM specimens and observation of
} TEM screen.
}
} Physics allows any positioning of a column in space; ergonomics speaks
} for traditional one, vertical with a gun on the top.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} } Sent: Thursday, May 28, 2009 7:40 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://www.microscopy.com/MLFormMail.html
} } --------------------------------------------------------------
} } -------------
} } Remember this posting is most likely not from a Subscriber, so when
} } replying
} } please copy both liina170-at-hot.ee as well as the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } -------------
} }
} } Email: liina170-at-hot.ee
} } Name: Liina
} }
} } Organization: University of Tartu
} }
} } Title-Subject: [Filtered] SEM and TEM
} }
} } Question: What would be the main advantages and disadvantages of a
} } SEM that operates upside down i.e. has e-gun at the bottom pointing
} } upwards. What about horizontal design? The same questions about TEM.
} }
} }
} } Login Host: 213.184.38.91
} } --------------------------------------------------------------
} } -------------
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 7, 11
} } -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n4SCcqfd009244
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} } 2009 07:38:53 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by way of
} } MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM and TEM Column
} } Orientation 7, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} } format="flowed"
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original
} Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 --
} Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu
} [134.193.143.167])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4SCwsIq007428
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
} 07:58:55 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Thu, 28 May 2009 07:58:53 -0500
} 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 --
} Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column
} Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 --
} Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column
} Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
} 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To:
} {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 --
} X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC)
} FILETIME=[0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4SCwsIq007428 ==============================End
} of - Headers==============================

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.


==============================Original
Headers==============================
7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009 7, 26 --
Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n4SDJ0tP023075
7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
08:19:01 -0500
7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- id
{01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
7, 26 -- Thu, 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Received: from
HOC1.ecr6.ohio-state.edu 7, 26 -- (hoc1.ceof.ohio-state.edu
[164.107.76.179]) by er6s1.ecr6.ohio-state.edu 7, 26 -- (PMDF V6.3-x18
#31556) 7, 26 -- with ESMTPA id
{01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu, 7, 26 -- 28 May 2009
09:18:58 -0400 (EDT) 7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400 7,
26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 26 -- Subject: Re:
[Microscopy] RE: viaWWW: SEM and TEM Column Orientation 7, 26 --
In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com}
7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 26 -- To:
DusevichV-at-umkc.edu 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 --
Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu}
7, 26 -- MIME-version: 1.0
7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 26 --
Content-type: text/plain; charset=us-ascii; format=flowed 7, 26 --
X-Env-From: auth/colijn.1-at-osu.edu 7, 26 -- References:
{200905281300.n4SD0xOu009804-at-ns.microscopy.com}
==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
22, 34 -- From David.Patton-at-uwe.ac.uk Thu May 28 08:24:46 2009
22, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65])
22, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4SDOi19032193
22, 34 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:24:45 -0500
22, 34 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp
22, 34 -- id 55a2_e7ad0b7c_4b8a_11de_bcf6_00142221cca9;
22, 34 -- Thu, 28 May 2009 14:24:43 +0100
22, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
22, 34 -- (egen-uwe01.uwe.ac.uk [164.11.249.121])
22, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
22, 34 -- 2005)) with ESMTP id {0KKC000NWVX618-at-mta02.uwe.ac.uk} for
22, 34 -- microscopy-at-microscopy.com; Thu, 28 May 2009 14:24:43 +0100 (BST)
22, 34 -- Date: Thu, 28 May 2009 14:24:23 +0100
22, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk}
22, 34 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
22, 34 -- In-reply-to: {200905281322.n4SDMjxZ028879-at-ns.microscopy.com}
22, 34 -- To: colijn.1-at-osu.edu
22, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF8528A-at-egen-uwe01}
22, 34 -- MIME-version: 1.0
22, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
22, 34 -- Content-type: text/plain; charset=us-ascii
22, 34 -- Content-class: urn:content-classes:message
22, 34 -- Thread-topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
22, 34 -- Thread-index: Acnfl2q6izn2N4XySqCGyuJVGsyDfgAABc1g
22, 34 -- X-MS-Has-Attach:
22, 34 -- X-MS-TNEF-Correlator:
22, 34 -- References: {200905281322.n4SDMjxZ028879-at-ns.microscopy.com}
22, 34 -- X-NAIMIME-Disclaimer: 1
22, 34 -- X-NAIMIME-Modified: 1
22, 34 -- X-NAI-Spam-Score: 0
22, 34 -- X-NAI-Spam-Rules: 1 Rules triggered
22, 34 -- RV3284=0
22, 34 -- Content-Transfer-Encoding: 8bit
22, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SDOi19032193
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 28 May 2009 08:30:07 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One of the early TEM was inverted. The column ran at an angle from the
floor so the operator could look down at the screen. Rumor has it, the
rocket ship control pannels in a Flash Gorden serial from the 30's starring
Buster Crabbe from the -30's used a non-working inverted TEM. I want to
guess it was a Semens, but it just a guess.



DusevichV-at-umkc.ed
u
To
05/28/2009 09:08 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] RE: viaWWW: SEM and
DusevichV-at-umkc.ed TEM Column Orientation
u












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


For SEM it would be very inconvenient to mount specimens upside down, in
addition all the dirt and dust will fall down in the direction of an
electron gun, contaminating it. TEM column is too tall to mount it
upside down, and fluorescence screen should be transparent. Horizontal
positioning will take much more space than vertical one, and again will
introduce inconvenience in mounting SEM specimens and observation of TEM
screen.

Physics allows any positioning of a column in space; ergonomics speaks
for traditional one, vertical with a gun on the top.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} Sent: Thursday, May 28, 2009 7:40 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://www.microscopy.com/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both liina170-at-hot.ee as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: liina170-at-hot.ee
} Name: Liina
}
} Organization: University of Tartu
}
} Title-Subject: [Filtered] SEM and TEM
}
} Question: What would be the main advantages and disadvantages
} of a SEM that operates upside down i.e. has e-gun at the
} bottom pointing upwards. What about horizontal design? The
} same questions about TEM.
}
}
} Login Host: 213.184.38.91
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n4SCcqfd009244
} 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} 2009 07:38:53 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by
} way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM
} and TEM Column Orientation 7, 11 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original
Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009
8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu
[134.193.143.167])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n4SCwsIq007428
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
07:58:55 -0500
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Thu, 28 May 2009 07:58:53 -0500
8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="us-ascii"
8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500
8, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) FILETIME=
[0D6DBFE0:01C9DF94]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n4SCwsIq007428
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
25, 22 -- From frank_karl-at-lincolnelectric.com Thu May 28 08:30:07 2009
25, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
25, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDU5an013659
25, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:30:06 -0500
25, 22 -- In-Reply-To: {200905281308.n4SD8gpL020571-at-ns.microscopy.com}
25, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation
25, 22 -- To: DusevichV-at-umkc.edu, Microscopy-at-microscopy.com
25, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
25, 22 -- Message-ID: {OF047B30F9.825D96CF-ON852575C4.00499E1E-852575C4.004A25E8-at-lincolnelectric.com}
25, 22 -- Date: Thu, 28 May 2009 09:30:00 -0400
25, 22 -- From: Frank_Karl-at-lincolnelectric.com
25, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 05/28/2009 09:29:53 AM,
25, 22 -- CD-MIME complete at 05/28/2009 09:29:54 AM,
25, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 05/28/2009 09:29:54 AM,
25, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 05/28/2009 09:29:54 AM,
25, 22 -- Serialize complete at 05/28/2009 09:29:54 AM
25, 22 -- MIME-Version: 1.0
25, 22 -- Content-Type: text/plain;
25, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 28 May 2009 08:32:23 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes. It makes just everything more complicated but INTRINSICALLY it is feasible!
You can also look at your samples with your head down and your feet up. It is possible and does not change the results, it is just not convenient!
It is not advised to lay down horizontally along the table during the observations neither :-) (but it is possible)

Stéphane



----- Original Message ----
X-from: "colijn.1-at-osu.edu" {colijn.1-at-osu.edu}
To: nizets2-at-yahoo.com
Sent: Thursday, May 28, 2009 3:23:12 PM

Not to mention that on an inverted column the electrons have to
overcome gravity to make their way from the gun to the sample!  {...grin...}

Henk

At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:


} For SEM it would be very inconvenient to mount specimens upside down, in
} addition all the dirt and dust will fall down in the direction of an
} electron gun, contaminating it. TEM column is too tall to mount it
} upside down, and fluorescence screen should be transparent. Horizontal
} positioning will take much more space than vertical one, and again will
} introduce inconvenience in mounting SEM specimens and observation of TEM
} screen.
}
} Physics allows any positioning of a column in space; ergonomics speaks
} for traditional one, vertical with a gun on the top.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax:  (816) 235-5524
} Web:    http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} } Sent: Thursday, May 28, 2009 7:40 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy
} } Society of America To  Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} } This Question/Comment was submitted to the Microscopy
} } Listserver using the WWW based Form at
} } http://www.microscopy.com/MLFormMail.html
} } --------------------------------------------------------------
} } -------------
} } Remember this posting is most likely not from a Subscriber,
} } so when replying
} } please  copy  both liina170-at-hot.ee as well as  the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } -------------
} }
} } Email: liina170-at-hot.ee
} } Name: Liina
} }
} } Organization: University of Tartu
} }
} } Title-Subject: [Filtered] SEM and TEM
} }
} } Question: What would be the main advantages and disadvantages
} } of a SEM that operates upside down i.e. has e-gun at the
} } bottom pointing upwards. What about horizontal design? The
} } same questions about TEM.
} }
} }
} }   Login Host: 213.184.38.91
} } --------------------------------------------------------------
} } -------------
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 --      by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n4SCcqfd009244
} } 7, 11 --      for {microscopy-at-microscopy.com} ; Thu, 28 May
} } 2009 07:38:53 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by
} } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM
} } and TEM Column Orientation 7, 11 -- Content-Type: text/plain;
} } charset="us-ascii" ; format="flowed"
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009
} 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu
} (smtp.exchange.umkc.edu [134.193.143.167])
} 8, 25 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4SCwsIq007428
} 8, 25 --        for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
} 07:58:55 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 --        Thu, 28 May 2009 07:58:53 -0500
} 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 --        charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500
} 8, 25 -- Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
} 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC)
} FILETIME=[0D6DBFE0:01C9DF94]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4SCwsIq007428
} ==============================End of - Headers==============================

Hendrik O. Colijn                      colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674                  http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009
7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
7, 26 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDJ0tP023075
7, 26 --     for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:19:01 -0500
7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
7, 26 --  er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
7, 26 --  id {01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
7, 26 --  Thu, 28 May 2009 09:18:58 -0400 (EDT)
7, 26 -- Received: from HOC1.ecr6.ohio-state.edu
7, 26 --  (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
7, 26 --  (PMDF V6.3-x18 #31556)
7, 26 --  with ESMTPA id {01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu,
7, 26 --  28 May 2009 09:18:58 -0400 (EDT)
7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400
7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
7, 26 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation
7, 26 -- In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com}
7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
7, 26 -- To: DusevichV-at-umkc.edu
7, 26 -- Cc: Microscopy-at-microscopy.com
7, 26 -- Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu}
7, 26 -- MIME-version: 1.0
7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
7, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
7, 26 -- References: {200905281300.n4SD0xOu009804-at-ns.microscopy.com}
==============================End of - Headers==============================






==============================Original Headers==============================
22, 24 -- From nizets2-at-yahoo.com Thu May 28 08:32:23 2009
22, 24 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231])
22, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4SDWKcd019506
22, 24 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:32:22 -0500
22, 24 -- Received: (qmail 34028 invoked by uid 60001); 28 May 2009 13:32:17 -0000
22, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243517537; bh=GELY6Y7CKH6CK3+B/0tEu0axgMhFfL3i12mNqfwGKNk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=QjuiI9n+d6CwKVl3teLmUH8vSydt43loICS5kJupmtwMVPIqie170sRMOneXjX+fQF473GI5lge6Rfuiy0PLDO7qWBqH96TzCtXXyJGHTen5OQuPgCI0d5lqcyJ9tvAth7EeyBt1JEiquD/w7hB9y3C0/5p2qBE1nQnBKVCXK7g=
22, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
22, 24 -- s=s1024; d=yahoo.com;
22, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
22, 24 -- b=dog4PKHgBzqsQQ17vMQPddhrLKf5DnIpWRJ6BUSCnZlRkjOyyv0ErPnkCLtKmAvwwajnteFpv2BrJ47HV8+GwLT/Cp5MOIGwY8tDGXnnj8Q52XCZhgs/rn5gTJxixYrdY58/RAT4cMUUvn9IfrO5w9qb59rXfqIPavjc71QaAyg=;
22, 24 -- Message-ID: {615381.33741.qm-at-web110808.mail.gq1.yahoo.com}
22, 24 -- X-YMail-OSG: Piw6cBYVM1ljMjYx7xGtj4nUDb018uoslRQOr6pcZq1cjIUgnB0wUbNckFr8fi6tJm4bnLMQJb48EFYq4fCEh6ga3a636XqnpQEzmGyVhFvCckug6oGNPldthlAbNKabGDA0yom37AuHJ3DRgqgv7W.MTi1x0jwnNEoCJSFcqR9OjtK9KGqIz7fhPoCzHV2qyYLFnl_f4_dUQVcB491UBUEJymXHqQIRc2NfY05ZDqv.4..LMQQTEesdAVY_30zo.Qfjlt18D.Phjt3ifOmdO4NtEdH2.RfNxdDeB.J.LWFUKHIWNP9f4TNWA6IB1A6m1ASZrVRS7mooXT0oDf._2I41K14W9d9YtkGCdkkKkw--
22, 24 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Thu, 28 May 2009 06:32:17 PDT
22, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
22, 24 -- References: {200905281323.n4SDNCPn029656-at-ns.microscopy.com}
22, 24 -- Date: Thu, 28 May 2009 06:32:17 -0700 (PDT)
22, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
22, 24 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation
22, 24 -- To: microscopy-at-microscopy.com
22, 24 -- In-Reply-To: {200905281323.n4SDNCPn029656-at-ns.microscopy.com}
22, 24 -- MIME-Version: 1.0
22, 24 -- Content-Type: text/plain; charset=iso-8859-1
22, 24 -- Content-Transfer-Encoding: 8bit
22, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SDWKcd019506
==============================End of - Headers==============================




From: jsb43-at-cam.ac.uk
Date: Thu, 28 May 2009 08:34:31 -0500
Subject: [Microscopy] Re: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This reminded me of a conversation I overheard between a couple of
Professors about how electrons behave in a gravitational field. The
Theoretician pointed out that, as a source of energy, gravity is extremely
coherent and would be a good for interferometry. The Experimentalist
pointed out that the electron column would have to be several hundred light
years long for TEM-like energies. Just don't ask how one would align the
gun of a microscope that long!


}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Not to mention that on an inverted column the electrons have to overcome
} gravity to make their way from the gun to the sample! {...grin...}
}
} Henk


==============================Original Headers==============================
4, 27 -- From jsb43-at-hermes.cam.ac.uk Thu May 28 08:34:30 2009
4, 27 -- Received: from ppsw-6.csi.cam.ac.uk (ppsw-6.csi.cam.ac.uk [131.111.8.136])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDYT50025885
4, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:34:30 -0500
4, 27 -- X-Cam-AntiVirus: no malware found
4, 27 -- X-Cam-SpamDetails: not scanned
4, 27 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
4, 27 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:45447)
4, 27 -- by ppsw-6.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25)
4, 27 -- with esmtpa (EXTERNAL:jsb43) id 1M9fkU-0006Ba-JN (Exim 4.70) for Microscopy-at-microscopy.com
4, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 May 2009 14:34:22 +0100
4, 27 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk)
4, 27 -- with local (PRAYER:jsb43) id 1M9fkT-0005fk-W0 (Exim 4.67) for Microscopy-at-microscopy.com
4, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 May 2009 14:34:21 +0100
4, 27 -- Received: from [131.111.102.18] by webmail.hermes.cam.ac.uk
4, 27 -- with HTTP (Prayer-1.3.1); 28 May 2009 14:34:21 +0100
4, 27 -- Date: 28 May 2009 14:34:21 +0100
4, 27 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk}
4, 27 -- To: MSA Listserver {Microscopy-at-microscopy.com}
4, 27 -- Subject: Re: SEM and TEM Column Orientation
4, 27 -- Message-ID: {Prayer.1.3.1.0905281434210.16334-at-hermes-2.csi.cam.ac.uk}
4, 27 -- In-Reply-To: {200905281323.n4SDNghq030392-at-ns.microscopy.com}
4, 27 -- References: {200905281323.n4SDNghq030392-at-ns.microscopy.com}
4, 27 -- X-Mailer: Prayer v1.3.1
4, 27 -- Mime-Version: 1.0
4, 27 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1
4, 27 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk}
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Thu, 28 May 2009 09:02:40 -0500
Subject: [Microscopy] SEM and TEM column orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a vintage brochure for the Corinth 500, which was an upside-down
TEM. You straddled the column, and the specimen exchange port was right
at crotch level! They must have had GREAT faith in their x-ray shielding!
A scan of the cover is at:

http://www.mta.ca/dmf/download/jme/corinth500.htm

Enjoy!

JME
--
- Does the name Pavlov ring a bell?
--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
7, 18 -- From jehrman-at-mta.ca Thu May 28 09:02:39 2009
7, 18 -- Received: from mailgate2.mta.ca (mailgate2.mta.ca [138.73.1.154])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SE2dqr029566
7, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:02:39 -0500
7, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:64907)
7, 18 -- by mailgate2.mta.ca (smtp.mta.ca [138.73.1.130]:25)
7, 18 -- with esmtp id 1M9gEk-00063X-7U (Exim 4.69) for Microscopy-at-microscopy.com
7, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 28 May 2009 11:05:38 -0300
7, 18 -- Message-ID: {4A1E98C6.20100-at-mta.ca}
7, 18 -- Date: Thu, 28 May 2009 10:59:34 -0300
7, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
7, 18 -- MIME-Version: 1.0
7, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
7, 18 -- Subject: SEM and TEM column orientation
7, 18 -- X-Enigmail-Version: 0.95.7
7, 18 -- Content-Type: text/plain; charset=ISO-8859-1
7, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jayma.moore-at-ndsu.edu
Date: Thu, 28 May 2009 09:11:48 -0500
Subject: [Microscopy] Inverted TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Liina,

I've seen two SEMs with tilted columns.

CamScan (UK) make the X500 crystal probe (http://www.camscan.com/X500.htm) -
optimised for EBSD analysis with the column tilted through 70°. The original
concept was to allow rocks to be heated to close to their melting point and
to avoid them dripping (or spalling) off the horizontal stage.

Visitec (Germany, http://www.visitec-em.de/ ) make Mira - an SEM with a
chamber large enough to stand inside and a column that moves around and
tilts (http://www.visitec-em.de/cms/en/?Products:Positioning_system). Mira
can look at very large specimens, e.g. satellites, car engines (there's even
a photo of the head of Terracotta warrior).

The main advantage is that unusual specimens & geometries can be used.

Hope this helps,

Austin

----- Original Message -----
X-from: {liina170-at-hot.ee}
To: {AuntDaisy-at-gmail.com}
Sent: Thursday, May 28, 2009 1:45 PM

Our laboratory purchased an AEI Corinth 275 TEM with an inverted column
in 1973 (before my time!) The director reported it was a beast to
align, manually, the seven steel rings that comprised the column; and
there were a series of frustrating problems beginning at installation
and continuing for three long years. Apparently, though, the optics
were quite good (7 Angstrom resolution), and the wood cabinet was
beautiful. The microscope remained in operation until 1981. There's
more information and a picture in the history section on our website, if
you are interested:
http://www.ndsu.nodak.edu/ndsu/em_lab/documents/History.pdf

Jayma Moore, DVM MS
Laboratory Manager
Electron Microscopy Center
North Dakota State University
Fargo


==============================Original Headers==============================
3, 19 -- From jayma.moore-at-ndsu.edu Thu May 28 09:11:47 2009
3, 19 -- Received: from smtp1.NoDak.edu (smtp1.NoDak.edu [134.129.111.50])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SEBkHt016364
3, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 May 2009 09:11:47 -0500
3, 19 -- Received: from [134.129.124.162] (dyn162.124.ndsu.NoDak.edu [134.129.124.162])
3, 19 -- (authenticated bits=0)
3, 19 -- by smtp1.NoDak.edu (8.13.1/8.13.1) with ESMTP id n4SEBk2F010229
3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
3, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 May 2009 09:11:46 -0500
3, 19 -- Message-ID: {4A1E9B9E.8040900-at-ndsu.edu}
3, 19 -- Date: Thu, 28 May 2009 09:11:42 -0500
3, 19 -- From: "Jayma A. Moore DVM" {jayma.moore-at-ndsu.edu}
3, 19 -- Organization: NDSU Electron Microscopy Center
3, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
3, 19 -- MIME-Version: 1.0
3, 19 -- To: Microscopy-at-Microscopy.Com
3, 19 -- Subject: Inverted TEM
3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Thu, 28 May 2009 09:18:16 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Actually there have been a number of horizontal/inclined and inverted
column microscopes. Some that I can come up with off the top of my head are:

All of the VG STEMs (inverted)
Nion STEMs (inverted)
Philips EM100 (inclined)
JEOL SuperScope (inclined, JEM-50?, 50kV?)
AEI Corinth (inverted)
Vickers EM4 (early 1950s?)
Shimadzu SM-C2 (horizontal, early 1950s?)

Can anyone else come up with any? How about photos?

Cheers,
Henk


At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:


} For SEM it would be very inconvenient to mount specimens upside down, in
} addition all the dirt and dust will fall down in the direction of an
} electron gun, contaminating it. TEM column is too tall to mount it
} upside down, and fluorescence screen should be transparent. Horizontal
} positioning will take much more space than vertical one, and again will
} introduce inconvenience in mounting SEM specimens and observation of TEM
} screen.
}
} Physics allows any positioning of a column in space; ergonomics speaks
} for traditional one, vertical with a gun on the top.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} } Sent: Thursday, May 28, 2009 7:40 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} } This Question/Comment was submitted to the Microscopy
} } Listserver using the WWW based Form at
} } http://www.microscopy.com/MLFormMail.html
} } --------------------------------------------------------------
} } -------------
} } Remember this posting is most likely not from a Subscriber,
} } so when replying
} } please copy both liina170-at-hot.ee as well as the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } -------------
} }
} } Email: liina170-at-hot.ee
} } Name: Liina
} }
} } Organization: University of Tartu
} }
} } Title-Subject: [Filtered] SEM and TEM
} }
} } Question: What would be the main advantages and disadvantages
} } of a SEM that operates upside down i.e. has e-gun at the
} } bottom pointing upwards. What about horizontal design? The
} } same questions about TEM.
} }
} }
} } Login Host: 213.184.38.91
} } --------------------------------------------------------------
} } -------------
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n4SCcqfd009244
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} } 2009 07:38:53 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by
} } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM
} } and TEM Column Orientation 7, 11 -- Content-Type: text/plain;
} } charset="us-ascii" ; format="flowed"
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009
} 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu
} (smtp.exchange.umkc.edu [134.193.143.167])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4SCwsIq007428
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
} 07:58:55 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Thu, 28 May 2009 07:58:53 -0500
} 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500
} 8, 25 -- Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
} 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC)
} FILETIME=[0D6DBFE0:01C9DF94]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4SCwsIq007428
} ==============================End of - Headers==============================

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
10, 22 -- From colijn.1-at-osu.edu Thu May 28 09:18:15 2009
10, 22 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SEIFud005161
10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:18:15 -0500
10, 22 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
10, 22 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 22 -- id {01N9HDHXTPQO8XEUCW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
10, 22 -- Thu, 28 May 2009 10:18:14 -0400 (EDT)
10, 22 -- Received: from HOC1.ecr6.ohio-state.edu
10, 22 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
10, 22 -- (PMDF V6.3-x18 #31556) with ESMTPA id {01N9HDHWLVVY8XKOCN-at-ecr6.ohio-state.edu}
10, 22 -- for Microscopy-at-microscopy.com; Thu, 28 May 2009 10:18:13 -0400 (EDT)
10, 22 -- Date: Thu, 28 May 2009 10:19:40 -0400
10, 22 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
10, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation
10, 22 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
10, 22 -- To: Microscopy-at-microscopy.com
10, 22 -- Message-id: {01N9HDHWOAIO8XKOCN-at-ecr6.ohio-state.edu}
10, 22 -- MIME-version: 1.0
10, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed
10, 22 -- X-Env-From: auth/colijn.1-at-osu.edu
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Thu, 28 May 2009 10:05:18 -0500
Subject: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,
I was asked to forward this to the list and thought, "why not?"

Stay safe




"cannonmp"
{cannonmp-at-comcast
.net} To
{Frank_Karl-at-lincolnelectric.com}
05/28/2009 10:28 cc
AM
Subject
Re: [Microscopy] viaWWW: SEM and
TEM Column Orientation










Hello Frank,

For some reason I can't post to the list even though I was a very early
member.

I believe that the RCA EM-100 may have been the first commercial TEM. Its
column was oriented at approximately 45 degrees to the floor. The screen
was viewed directly like a television set. The cabinet was a cast and
polished console which did indeed look like something out of a 1940s space
movie.

I bought one of these at a surplus sale in 1984, and like a pure idiot I
scrapped the console. It was GORGEOUS and I regret that decision to this
day.

Think of what a fantastic cocktail bar that console would have made.

Forward to the list if you can.

Bart Cannon
Cannon Microprobe
Seattle
----- Original Message -----
X-from: {Frank_Karl-at-lincolnelectric.com}
To: {cannonmp-at-comcast.net}
Sent: Thursday, May 28, 2009 6:39 AM

Hello,
there is a quite recent device LVEM5 produced by Delong Instruments. It is a
low voltage electron microscope and it is "true inverted".
Best regards from Prague
Oldrich

P.S. I'm not sure if I may post here a WEBlink to DI company. They have there
a nice image of it and a lot of info.


On Thursday 28 of May 2009 16:20:37 colijn.1-at-osu.edu wrote:
} ---------------------------------------------------------------------------
} - The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------
} -
}
} Actually there have been a number of horizontal/inclined and inverted
} column microscopes. Some that I can come up with off the top of my head
} are:
}
} All of the VG STEMs (inverted)
} Nion STEMs (inverted)
} Philips EM100 (inclined)
} JEOL SuperScope (inclined, JEM-50?, 50kV?)
} AEI Corinth (inverted)
} Vickers EM4 (early 1950s?)
} Shimadzu SM-C2 (horizontal, early 1950s?)
}
} Can anyone else come up with any? How about photos?
}
} Cheers,
} Henk


==============================Original Headers==============================
5, 23 -- From benada-at-biomed.cas.cz Thu May 28 10:05:17 2009
5, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SF5HEX004591
5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 10:05:17 -0500
5, 23 -- Received: from ob117nb.localnet (u117ob.mbu.cas.cz [147.231.44.101])
5, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
5, 23 -- (No client certificate requested)
5, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id A51A21A4452B
5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 17:05:16 +0200 (CEST)
5, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
5, 23 -- Organization: Institute of Microbiology
5, 23 -- To: microscopy-at-microscopy.com
5, 23 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation
5, 23 -- Date: Thu, 28 May 2009 17:04:41 +0200
5, 23 -- User-Agent: KMail/1.11.2 (Linux/2.6.29-2-686; KDE/4.2.2; i686; ; )
5, 23 -- References: {200905281420.n4SEKbwS010884-at-ns.microscopy.com}
5, 23 -- In-Reply-To: {200905281420.n4SEKbwS010884-at-ns.microscopy.com}
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Type: Text/Plain;
5, 23 -- charset="utf-8"
5, 23 -- Content-Transfer-Encoding: 7bit
5, 23 -- Content-Disposition: inline
5, 23 -- Message-Id: {200905281704.41764.benada-at-biomed.cas.cz}
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 28 May 2009 11:24:33 -0500
Subject: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On May 28, 2009, at 5:39 AM, liina170-at-hot.ee wrote:

} What would be the main advantages and disadvantages of a
} SEM that operates upside down i.e. has e-gun at the bottom pointing
} upwards. What about horizontal design? The same questions about TEM.


Dear Liina,
A big disadvantage that no one has yet mentioned for TEM is that
brehmsstrahlung x-rays are forward directed and can have the same
energy as the beam electrons. Since TEMs are usually placed in rooms
that are on the lowest floor, the conventional orientation causes most
of the radiation to be directed harmlessly into the earth; whereas, an
inverted orientation would direct them into the floor above. For
lower voltage instruments, the intervening ceiling and floor might be
enough to absorb the radiation, but for higher voltage instruments,
there could be an unsafe level of radiation that makes it into the
room above the scope. For the HVEM in Albany, NY, we were very
concerned about radiation scattering in various directions, since a
1.2 MeV photon will traverse a few meters of lead.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
6, 22 -- From tivol-at-caltech.edu Thu May 28 11:24:33 2009
6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SGOW59023112
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:24:33 -0500
6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 8170766E471F
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:32 -0700 (PDT)
6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146])
6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 8F2E566E4720
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:31 -0700 (PDT)
6, 22 -- Message-Id: {5346CC5F-3A8C-42FB-B929-914A48421F67-at-caltech.edu}
6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
6, 22 -- To: microscopy-at-microscopy.com
6, 22 -- In-Reply-To: {200905281239.n4SCd1ai009416-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
6, 22 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation
6, 22 -- Date: Thu, 28 May 2009 09:24:31 -0700
6, 22 -- References: {200905281239.n4SCd1ai009416-at-ns.microscopy.com}
6, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Thu, 28 May 2009 11:33:55 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Years ago ( won't say how many as that might give away my age...and I try to
think young!!) I worked in the laboratory of Humberto Fernandez-Moran at the
U of Chicago. Moran had a large scar on his nose where he had a cancerous
lesion removed. He swore that it was from pressing his nose against the
window of a TEM before TEM windows were lead-coated to prevent release of
radiation.

This response just triggered the memory.....
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/


} From: Bill Tivol {tivol-at-caltech.edu}
} Reply-To: Bill Tivol {tivol-at-caltech.edu}
} Date: Thu, 28 May 2009 11:26:42 -0500
} To: Debby Sherman {dsherman-at-purdue.edu}
} Subject: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} On May 28, 2009, at 5:39 AM, liina170-at-hot.ee wrote:
}
} } What would be the main advantages and disadvantages of a
} } SEM that operates upside down i.e. has e-gun at the bottom pointing
} } upwards. What about horizontal design? The same questions about TEM.
}
}
} Dear Liina,
} A big disadvantage that no one has yet mentioned for TEM is that
} brehmsstrahlung x-rays are forward directed and can have the same
} energy as the beam electrons. Since TEMs are usually placed in rooms
} that are on the lowest floor, the conventional orientation causes most
} of the radiation to be directed harmlessly into the earth; whereas, an
} inverted orientation would direct them into the floor above. For
} lower voltage instruments, the intervening ceiling and floor might be
} enough to absorb the radiation, but for higher voltage instruments,
} there could be an unsafe level of radiation that makes it into the
} room above the scope. For the HVEM in Albany, NY, we were very
} concerned about radiation scattering in various directions, since a
} 1.2 MeV photon will traverse a few meters of lead.
} Yours,
} Bill Tivol, PhD
} EM Scientist
} Ultrafast EM Facility
} Noyes Laboratory, MC 127-72
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
} ==============================Original Headers==============================
} 6, 22 -- From tivol-at-caltech.edu Thu May 28 11:24:33 2009
} 6, 22 -- Received: from outgoing-mail.its.caltech.edu
} (outgoing-mail.its.caltech.edu [131.215.239.19])
} 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n4SGOW59023112
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:24:33 -0500
} 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1])
} 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 8170766E471F
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:32 -0700
} (PDT)
} 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new
} 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu
} [131.215.19.146])
} 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 8F2E566E4720
} 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:31 -0700
} (PDT)
} 6, 22 -- Message-Id: {5346CC5F-3A8C-42FB-B929-914A48421F67-at-caltech.edu}
} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
} 6, 22 -- To: microscopy-at-microscopy.com
} 6, 22 -- In-Reply-To: {200905281239.n4SCd1ai009416-at-ns.microscopy.com}
} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 6, 22 -- Content-Transfer-Encoding: 7bit
} 6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
} 6, 22 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation
} 6, 22 -- Date: Thu, 28 May 2009 09:24:31 -0700
} 6, 22 -- References: {200905281239.n4SCd1ai009416-at-ns.microscopy.com}
} 6, 22 -- X-Mailer: Apple Mail (2.935.3)
} ==============================End of - Headers==============================


==============================Original Headers==============================
5, 31 -- From dsherman-at-purdue.edu Thu May 28 11:33:55 2009
5, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SGXto5004504
5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:33:55 -0500
5, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
5, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n4SGXsiK018742
5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:33:54 -0400
5, 31 -- Received: from 1061exfe03a.purdue.lcl (1061exfe03a.itap.purdue.edu [128.210.1.10])
5, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n4SGXsQk012678
5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:33:54 -0400
5, 31 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe03a.purdue.lcl with Microsoft SMTPSVC(6.0.3790.3959);
5, 31 -- Thu, 28 May 2009 12:33:54 -0400
5, 31 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ;
5, 31 -- Thu, 28 May 2009 16:33:07 +0000
5, 31 -- User-Agent: Microsoft-Entourage/12.15.0.081119
5, 31 -- Date: Thu, 28 May 2009 12:33:06 -0400
5, 31 -- Subject: Re: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation
5, 31 -- From: Debby Sherman {dsherman-at-purdue.edu}
5, 31 -- To: Bill Tivol {tivol-at-caltech.edu} ,
5, 31 -- "message to: MSA list" {microscopy-at-microscopy.com}
5, 31 -- Message-ID: {C6443502.3D7B7%dsherman-at-exchange.purdue.edu}
5, 31 -- Thread-Topic: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation
5, 31 -- Thread-Index: Acnfsfpmth/eH0j0RW2EstDwM8XAXw==
5, 31 -- In-Reply-To: {200905281626.n4SGQgBv026261-at-ns.microscopy.com}
5, 31 -- Mime-version: 1.0
5, 31 -- Content-type: text/plain;
5, 31 -- charset="US-ASCII"
5, 31 -- Content-transfer-encoding: 7bit
5, 31 -- X-OriginalArrivalTime: 28 May 2009 16:33:54.0477 (UTC) FILETIME=[174B8DD0:01C9DFB2]
5, 31 -- X-PMX-Version: 5.4.0.320885
5, 31 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: joelsheffield-at-gmail.com
Date: Thu, 28 May 2009 12:04:55 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I picked up this thread a bit late, so please excuse me for any duplication. I cut my eyeteeth, as it
were, on a Phillips EM100, which had a horizontal column with a very thick viewing screen right up
against my face. The instrument looked like a front loading washing machine In order to align the
column, you had to mount a mirror on the wall so that you could get behind the instrument and
see the images on the screen. A few years ago, I came across one of these in the Science
Museum in Delft. How strange to see something I worked with, and published images from,
sitting stripped down as an historical artifact.

No evidence of any problems yet (45 years ago), but I always wondered about radiation exposure.
--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
3, 39 -- From joelsheffield-at-gmail.com Thu May 28 12:04:54 2009
3, 39 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.144])
3, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SH4sJi020284
3, 39 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:04:54 -0500
3, 39 -- Received: by qw-out-1920.google.com with SMTP id 4so1658796qwk.54
3, 39 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 10:04:53 -0700 (PDT)
3, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 39 -- d=gmail.com; s=gamma;
3, 39 -- h=domainkey-signature:received:received:from:to:date:mime-version
3, 39 -- :subject:message-id:priority:x-mailer:content-type
3, 39 -- :content-transfer-encoding:content-description;
3, 39 -- bh=AWWPTd2YI+PBNE4RiNUbWDC/BKXcl1/d6XsWpvBy6LM=;
3, 39 -- b=CyYX88H9dBgSlZSFLtRqVlQEzeRZCc09/hRUHiNH2YfdMwV+jnUKi2pZOPcyPC6r8k
3, 39 -- KSZbE1B0t9mGyqO7pz5dypsrnxl1TJEM7Z6lUT8hnhIgfqrbx8dkYQ9qJBHs8tbLebID
3, 39 -- nl1KDgLQ27sHB4+dtLfsYZbsfgqBsHkKiSeu0=
3, 39 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 39 -- d=gmail.com; s=gamma;
3, 39 -- h=from:to:date:mime-version:subject:message-id:priority:x-mailer
3, 39 -- :content-type:content-transfer-encoding:content-description;
3, 39 -- b=osiLyqiRjsGzI0FTweQ0Us1/9kLZQFKaiG3wENYyhQdVATwoj2JEAH6od9R82jcG/k
3, 39 -- fuheJqFa9D3pvnbRO4Jma193GU0HfOG94YkvBuFjh+dBdzU/qCPEuCFvPzt0eTOm97pz
3, 39 -- rZJ2m9YGfExJZoS3JPQleW4AkwFbZeAwksHnk=
3, 39 -- Received: by 10.229.80.137 with SMTP id t9mr814480qck.68.1243530293049;
3, 39 -- Thu, 28 May 2009 10:04:53 -0700 (PDT)
3, 39 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40])
3, 39 -- by mx.google.com with ESMTPS id 4sm684062yxq.44.2009.05.28.10.04.52
3, 39 -- (version=SSLv3 cipher=RC4-MD5);
3, 39 -- Thu, 28 May 2009 10:04:52 -0700 (PDT)
3, 39 -- From: joelsheffield-at-gmail.com
3, 39 -- To: microscopy-at-microscopy.com
3, 39 -- Date: Thu, 28 May 2009 13:05:18 -0400
3, 39 -- MIME-Version: 1.0
3, 39 -- Subject: Re: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation
3, 39 -- Message-ID: {4A1E8C0E.10390.4367B3F-at-joelsheffield.gmail.com}
3, 39 -- Priority: normal
3, 39 -- X-mailer: Pegasus Mail for Windows (4.41)
3, 39 -- Content-type: text/plain; charset=US-ASCII
3, 39 -- Content-transfer-encoding: 7BIT
3, 39 -- Content-description: Mail message body
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Tuesday, May 26, 2009 4:24 pm
Subject: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

After reading about this for a few months, I decided to actually call our Occupational Safety department for some facts. Although we are all scientists, this is not our actual job training. Our representative DID NOT FIND ANY recorded deaths from single compressed nitrogen or carbon dioxide gas tank failure. (If you know of any, feel free to share.)

Our microscopes are all in lab-rated rooms. The ventilation system refreshes the air 6-10 times per hour by law (depending on the exact room settings). The largest compressed nitrogen tank (L1) holds 300 cubic feet of air. Our confocal microscope room is 8x16x8 feet in dimensions; which equals 1,024 cubic feet. If that tank suffers catastrophic failure, the user goes from 20.9% oxygen to 14.8% oxygen. The gas will mix with the room air quickly, and the ratio will return to 20% in 10 minutes or less.

LIQUID NITROGEN HAS killed people, and here's the difference: One liter of liquid nitrogen expands to 696 liters of nitrogen gas! In addition, that nitrogen will mix with the room air more slowly due to the cooler temperature of the nitrogen.

Our unoccupied rooms are still rated at a total air replacement rate of 4 times per hour. Even in a small, airtight room the conditions for suffocation are low. Anything's possible, but I think we can leave the issue of dry nitrogen gas versus mixed gas up to the individual purchaser. Safety is not the overriding factor.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Wednesday, May 27, 2009 5:00 AM
To: Sobocinski, Gregg

Dale

I'm sorry but there are too many "mosts" and "probablys" in your
argument and to even say that oxygen content is approaching death zone
levels makes the basic assumption that any victim is a fit
acclimatized mountain climber to be relatively safe.

Risk assessment must look at the worst scenario anyway and assume that
someone may enter the room after the noisy leakage event occurred.
They would have no warning and would almost certainly be unaware of
what was happening to them. They might be the cleaner or an
inexperienced student and be in the room alone before/after anybody
else is in the building.

People do die in workplace/lab situations from oxygen depletion and
there is always the risk that rescue attempts can put more people at
risk.

So I would say do the risk assessment for your workplace looking at
the worst leakage and find out what would be considered a safe and
acceptable level of oxygen (certainly not as low as 12%). If your lab
falls below those requirements then do something such as better
ventilation, smaller source of gas or an oxygen depletion monitor with
an alarm.

Cheers

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: dac-at-research.umass.edu

==============================Original Headers==============================
12, 27 -- From malcolm.haswell-at-sunderland.ac.uk Wed May 27 03:49:32 2009
12, 27 -- Received: from max2.sunderland.ac.uk (max2.sunderland.ac.uk [157.228.98.76])
12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4R8nVeO018508
12, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 03:49:31 -0500
12, 27 -- Received: (qmail 1804 invoked from network); 27 May 2009 08:44:26 -0000
12, 27 -- Received: from localhost (127.0.0.1)
12, 27 -- by max2.sunderland.ac.uk with SMTP; 27 May 2009 08:44:26 -0000
12, 27 -- Received: (qmail 1742 invoked by uid 599); 27 May 2009 08:44:20 -0000
12, 27 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117)
12, 27 -- by max2.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 27 May 2009 09:44:20 +0100
12, 27 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed,
12, 27 -- 27 May 2009 09:44:21 +0100
12, 27 -- Date: Wed, 27 May 2009 09:44:21 +0100
12, 27 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
12, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a problem?)
12, 27 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
12, 27 -- Cc: dac-at-research.umass.edu
12, 27 -- Message-id: {241418243950.243950241418-at-sunderland.ac.uk}
12, 27 -- MIME-version: 1.0
12, 27 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004)
12, 27 -- Content-type: text/plain; charset=us-ascii
12, 27 -- Content-language: en
12, 27 -- Content-transfer-encoding: 7BIT
12, 27 -- Content-disposition: inline
12, 27 -- X-Accept-Language: en
12, 27 -- Priority: normal
12, 27 -- X-Virus-Scanned: by iCritical at max2.sunderland.ac.uk
==============================End of - Headers==============================


==============================Original Headers==============================
22, 31 -- From greggps-at-umich.edu Thu May 28 13:10:14 2009
22, 31 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SIADRa004499
22, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 13:10:14 -0500
22, 31 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.234]) by
22, 31 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Thu, 28 May
22, 31 -- 2009 14:10:12 -0400
22, 31 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
22, 31 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
22, 31 -- Date: Thu, 28 May 2009 14:10:08 -0400
22, 31 -- Subject: Nitrogen gas leaks are NOT lethal in a lab
22, 31 -- Thread-Topic: Nitrogen gas leaks are NOT lethal in a lab
22, 31 -- Thread-Index: AcneqXqJF7NIcxAuTnWAj+cXcyHw+QAKCbXA
22, 31 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA39340611A6154F-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
22, 31 -- References: {200905270859.n4R8xfuk030739-at-ns.microscopy.com}
22, 31 -- In-Reply-To: {200905270859.n4R8xfuk030739-at-ns.microscopy.com}
22, 31 -- Accept-Language: en-US
22, 31 -- Content-Language: en-US
22, 31 -- X-MS-Has-Attach:
22, 31 -- X-MS-TNEF-Correlator:
22, 31 -- x-cr-puzzleid: {89223E7D-1660-4E8D-83E4-582F72E82DA7}
22, 31 -- x-cr-hashedpuzzle: Amm/ ChDg Colp CyUw DIbN DW9u EHGq EJ/H Eki1 GL03 G5Bh
22, 31 -- HsSE IBXc IKxe IP1O
22, 31 -- IeOS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{89223E7D-1660-4E8D-83E4-582F72E82DA7};ZwByAGUAZwBnAHAAcwBAAHUAbQBpAGMAaAAuAGUAZAB1AA==;Thu,
22, 31 -- 28 May 2009 18:10:08
22, 31 -- GMT;TgBpAHQAcgBvAGcAZQBuACAAZwBhAHMAIABsAGUAYQBrAHMAIABhAHIAZQAgAE4ATwBUACAAbABlAHQAaABhAGwAIABpAG4AIABhACAAbABhAGIA
22, 31 -- acceptlanguage: en-US
22, 31 -- Content-Type: text/plain; charset="us-ascii"
22, 31 -- MIME-Version: 1.0
22, 31 -- Content-Transfer-Encoding: 8bit
22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SIADRa004499
==============================End of - Headers==============================




From: mckee-at-helix.mgh.harvard.edu
Date: Thu, 28 May 2009 17:44:51 -0500
Subject: [Microscopy] viaWWW: fixation of cAMP for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mckee-at-helix.mgh.harvard.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mckee-at-helix.mgh.harvard.edu
Name: Mary McKee

Organization: MGH

Title-Subject: [Filtered] fixation of cAMP for EM

Question: Good morning,

Does anyone have a protocol for fixing cAMP in tissue culture cells?
I've heard something about microwave fixation. Thanks in advance.

Mary

Login Host: 132.183.122.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu May 28 17:44:50 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SMioZr029658
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 17:44:50 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c644c44cd82b-at-[206.69.208.22]}
8, 11 -- Date: Thu, 28 May 2009 17:44:49 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mckee-at-helix.mgh.harvard.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: fixation of cAMP for EM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 29 May 2009 01:54:09 -0500
Subject: [Microscopy] fixation of cAMP for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} Does anyone have a protocol for fixing cAMP in tissue culture cells?
} I've heard something about microwave fixation. Thanks in advance.

Hi Mary,

I am just thinking about three ways:
1. physical, cryofixation, plus freeze-substitution-fixation: I would favor this route. The question is then: does cAMP react with any of the 'chemicals' in the FSF solution, and at which temperature? And: how to detect the product of this further reaction? if you do NOT add any chemical fixative: how to specifically detect or visualize pure cAMP as such?
2+3, chemical, with or without microwave (the first way is worth testing!):
2. chemical, by GA: it appears plausible to me that cAMP can react with aldehydes - whether it really does, may depend on a variety of parameters, though. Is cAMP bound to an enzyme, and is it really freely accessible to the aldehyde? and then, when it has reacted, the cAMP molecule is changed, presumably the base itself. How to specifically detect and visualize?
3. chemical, by OsO4: again, cAMP may react with this chemical. How does it react, and what is the product? how to detect it, selectively? if Os(2) is bound to it, I would guess it is merely a 'black dot', 1 nm in size, in a 120keV machine. In fact, invisible ....

What do others think about this? would be interesting to know.

best regards
Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
9, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri May 29 01:54:07 2009
9, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4T6s6Z0026176
9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 01:54:06 -0500
9, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
9, 26 -- by localhost (Postfix) with SMTP id 0588DABF39
9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 08:54:08 +0200 (CEST)
9, 26 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
9, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id E580BABF3B
9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 08:54:07 +0200 (CEST)
9, 26 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
9, 26 -- with Novell_GroupWise; Fri, 29 May 2009 08:54:05 +0200
9, 26 -- Message-Id: {4A1FA2A9020000540001543C-at-gwsmtp1.uni-regensburg.de}
9, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
9, 26 -- Date: Fri, 29 May 2009 08:54:01 +0200
9, 26 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
9, 26 -- To: {mckee-at-helix.mgh.harvard.edu}
9, 26 -- Cc: {microscopy-at-microscopy.com}
9, 26 -- Subject: fixation of cAMP for EM
9, 26 -- References: {200905282245.n4SMjCTY030020-at-ns.microscopy.com}
9, 26 -- In-Reply-To: {200905282245.n4SMjCTY030020-at-ns.microscopy.com}
9, 26 -- Mime-Version: 1.0
9, 26 -- Content-Type: text/plain; charset=US-ASCII
9, 26 -- Content-Disposition: inline
9, 26 -- Content-Transfer-Encoding: 8bit
9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4T6s6Z0026176
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Fri, 29 May 2009 03:49:49 -0500
Subject: [Microscopy] viaWWW: fixation of cAMP for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

Here is a paper in light microscopy (full freely available online).

http://www.jbc.org/cgi/content/full/272/50/31489

Please note the remark about the difficulty to use classical aldehyde fixatives to immobilize cAMP.
They also refer to microwave fixation, stating that it has reproducibility issues (which is no reason not to try it :-)).
It may be that acrolein is not sufficient to maintain a good overall morphology for EM. In this case I don't see why you could not try to cumulate acrolein (for cAMP) and aldehyde (for ultrastructure) fixation. However one has generally to accept a degradation of the morphology to gain signal by immunodetection.
Please also note the possibility to inject conjugated PKA to reveal cAMP. By choosing wisely the conjugate you may be able to use this technique for detection in EM.

Cryo-EM (I mean with cryo-observation, aka CEMOVIS method) is always an option, but it is not widely available.

Regards,

Stephane



 


----- Original Message ----
X-from: "mckee-at-helix.mgh.harvard.edu" {mckee-at-helix.mgh.harvard.edu}
To: nizets2-at-yahoo.com
Sent: Friday, May 29, 2009 12:49:24 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both mckee-at-helix.mgh.harvard.edu as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mckee-at-helix.mgh.harvard.edu
Name: Mary McKee

Organization: MGH

Title-Subject: [Filtered] fixation of cAMP for EM

Question: Good morning,

Does anyone have a protocol for fixing cAMP in tissue culture cells?
I've heard something about microwave fixation.  Thanks in advance.

Mary

  Login Host: 132.183.122.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu May 28 17:44:50 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SMioZr029658
8, 11 --     for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 17:44:50 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c644c44cd82b-at-[206.69.208.22]}
8, 11 -- Date: Thu, 28 May 2009 17:44:49 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mckee-at-helix.mgh.harvard.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: fixation of cAMP for EM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
30, 25 -- From nizets2-at-yahoo.com Fri May 29 03:49:49 2009
30, 25 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231])
30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4T8nmE7012921
30, 25 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 03:49:48 -0500
30, 25 -- Received: (qmail 15786 invoked by uid 60001); 29 May 2009 08:49:48 -0000
30, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243586988; bh=XSowHSJBhgiyfKPHJFD8TD1qdBb85OIKJmUqfdI1e0k=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=XBalOmVxDNstqK3dKufgzP2hYKtf7xJt2lYFmonpX4F71qWJ0lQX0csGVUSyoZ8cn5YBL71UQb+Lg2DY+WwyXB5EkzIodPQwoOijpsMiJdI7BQQ5eo8aM9+f+MhAVcL2D3wj0IzbtGIu9LKoKLTkd9/zmuXxovGU+zj1DfiXHxE=
30, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
30, 25 -- s=s1024; d=yahoo.com;
30, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
30, 25 -- b=rZFbskZzUjMpxFtwy+a4Ttxuww2+84IqosoyqLkagiVBuWCtpyY6Joo6B4SFFD9IU2v+wAaVqHy3r3ltam6xzk4qE5JRsUFL6z/hbL6NokoQJpRZcKVoBL8HmlSKwdi0uRLgLVYdCeVYEGcrQiPQ21o4IrzvFkX3U9NwH1NPE4s=;
30, 25 -- Message-ID: {361688.15745.qm-at-web110808.mail.gq1.yahoo.com}
30, 25 -- X-YMail-OSG: 2vMJ_lsVM1mJRV02N_55UykuYZbw1hmlvK2vui8DtUyW3Ys0tJNKKg1D68rhGMcloUuJ1X3Bckt7OrVMpAdkwRUX0z9skXc2QfrzH_jK8ckCxh.FW8HrUejBpb_v85PuYKV39Eir67pbDHX1n.dvRzejkZoY8yyt9gfCykeN81LzX0PgNT0RRcUctln1vuPtrbQ4WldPx7XaAFqgpaCXcyYB66TWsDM1u6B5QTI5CQ47X8Vjc19JxzCh7YxpFA5jFSpEOWY0i3bWIK5sdqGGQb0SwLzx4ijlci496bVkHpqJwUqxAAJZ1FJgUB9vQ0fjPpA6k7OgvghFlQ_iyjbr28X05a3AGqdINePWOhZs.w--
30, 25 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Fri, 29 May 2009 01:49:48 PDT
30, 25 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10
30, 25 -- References: {200905282249.n4SMnOOP003806-at-ns.microscopy.com}
30, 25 -- Date: Fri, 29 May 2009 01:49:48 -0700 (PDT)
30, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
30, 25 -- Subject: Re: [Microscopy] viaWWW: fixation of cAMP for EM
30, 25 -- To: mckee-at-helix.mgh.harvard.edu
30, 25 -- Cc: microscopy-at-microscopy.com
30, 25 -- In-Reply-To: {200905282249.n4SMnOOP003806-at-ns.microscopy.com}
30, 25 -- MIME-Version: 1.0
30, 25 -- Content-Type: text/plain; charset=iso-8859-1
30, 25 -- Content-Transfer-Encoding: 8bit
30, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4T8nmE7012921
==============================End of - Headers==============================




From: Derek.Simpson-at-fei.com
Date: Fri, 29 May 2009 07:49:05 -0500
Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cambridge Instruments manufactured the Geoscan and the Microscan MKV in
the late 60's both of which had horizontal columns. They were SEM's with
two large fully focusing WDX.



Regards
Derek Simpson




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Thursday, May 28, 2009 10:21 AM
To: Simpson, Derek

Actually there have been a number of horizontal/inclined and inverted
column microscopes. Some that I can come up with off the top of my head
are:

All of the VG STEMs (inverted)
Nion STEMs (inverted)
Philips EM100 (inclined)
JEOL SuperScope (inclined, JEM-50?, 50kV?) AEI Corinth (inverted)
Vickers EM4 (early 1950s?) Shimadzu SM-C2 (horizontal, early 1950s?)

Can anyone else come up with any? How about photos?

Cheers,
Henk


At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:


} For SEM it would be very inconvenient to mount specimens upside down,
} in addition all the dirt and dust will fall down in the direction of an

} electron gun, contaminating it. TEM column is too tall to mount it
} upside down, and fluorescence screen should be transparent. Horizontal
} positioning will take much more space than vertical one, and again will

} introduce inconvenience in mounting SEM specimens and observation of
} TEM screen.
}
} Physics allows any positioning of a column in space; ergonomics speaks
} for traditional one, vertical with a gun on the top.
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee]
} } Sent: Thursday, May 28, 2009 7:40 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation
} }
} }
} }
} }
} } --------------------------------------------------------------
} } --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } --------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://www.microscopy.com/MLFormMail.html
} } --------------------------------------------------------------
} } -------------
} } Remember this posting is most likely not from a Subscriber, so when
} } replying
} } please copy both liina170-at-hot.ee as well as the
} } MIcroscopy Listserver
} } --------------------------------------------------------------
} } -------------
} }
} } Email: liina170-at-hot.ee
} } Name: Liina
} }
} } Organization: University of Tartu
} }
} } Title-Subject: [Filtered] SEM and TEM
} }
} } Question: What would be the main advantages and disadvantages of a
} } SEM that operates upside down i.e. has e-gun at the bottom pointing
} } upwards. What about horizontal design? The same questions about TEM.
} }
} }
} } Login Host: 213.184.38.91
} } --------------------------------------------------------------
} } -------------
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 7, 11
} } -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} } with ESMTP id n4SCcqfd009244
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May
} } 2009 07:38:53 -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]}
} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To:
} } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by way of
} } MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM and TEM Column
} } Orientation 7, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} } format="flowed"
} } ==============================End of -
} } Headers==============================
} }
} }
}
}
} ==============================Original
} Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 --
} Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu
} [134.193.143.167])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4SCwsIq007428
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
} 07:58:55 -0500
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
} kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Thu, 28 May 2009 07:58:53 -0500
} 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 --
} Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column
} Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 --
} Message-ID:
} {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column
} Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA
} 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To:
} {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 --
} X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC)
} FILETIME=[0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4SCwsIq007428 ==============================End
} of - Headers==============================

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.


==============================Original
Headers==============================
10, 22 -- From colijn.1-at-osu.edu Thu May 28 09:18:15 2009 10, 22 --
Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n4SEIFud005161
10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009
09:18:15 -0500
10, 22 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
10, 22 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 10, 22 --
id {01N9HDHXTPQO8XEUCW-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com; 10, 22 -- Thu, 28 May 2009 10:18:14 -0400
(EDT) 10, 22 -- Received: from HOC1.ecr6.ohio-state.edu 10, 22 --
(hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
10, 22 -- (PMDF V6.3-x18 #31556) with ESMTPA id
{01N9HDHWLVVY8XKOCN-at-ecr6.ohio-state.edu}
10, 22 -- for Microscopy-at-microscopy.com; Thu, 28 May 2009 10:18:13
-0400 (EDT) 10, 22 -- Date: Thu, 28 May 2009 10:19:40 -0400 10, 22 --
X-from: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 22 -- Subject: Re:
[Microscopy] RE: viaWWW: SEM and TEM Column Orientation 10, 22 --
Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 22 -- To:
Microscopy-at-microscopy.com 10, 22 -- Message-id:
{01N9HDHWOAIO8XKOCN-at-ecr6.ohio-state.edu}
10, 22 -- MIME-version: 1.0
10, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 22 --
Content-type: text/plain; charset=us-ascii; format=flowed 10, 22 --
X-Env-From: auth/colijn.1-at-osu.edu ==============================End of -
Headers==============================


==============================Original Headers==============================
24, 31 -- From Derek.Simpson-at-fei.com Fri May 29 07:49:05 2009
24, 31 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83])
24, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4TCn3L9005875
24, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 07:49:04 -0500
24, 31 -- X-WSS-ID: 0KKEOXQ-01-P2H-02
24, 31 -- X-M-MSG:
24, 31 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134])
24, 31 -- by smtp.feico.com (Tumbleweed MailGate 3.6.1) with ESMTP id 2CBEA82F7E7;
24, 31 -- Fri, 29 May 2009 05:49:02 -0700 (PDT)
24, 31 -- Received: from hlexc04.w2k.feico.com ([10.150.40.55]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959);
24, 31 -- Fri, 29 May 2009 05:49:00 -0700
24, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 31 -- Content-class: urn:content-classes:message
24, 31 -- MIME-Version: 1.0
24, 31 -- Content-Type: text/plain;
24, 31 -- charset="us-ascii"
24, 31 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation
24, 31 -- Date: Fri, 29 May 2009 05:47:58 -0700
24, 31 -- Message-ID: {A6E613CC1F9FD34B9A5EEE7DC4590AE302522DB0-at-hlexc04.w2k.feico.com}
24, 31 -- In-Reply-To: {200905281420.n4SEKo3Q011265-at-ns.microscopy.com}
24, 31 -- X-MS-Has-Attach:
24, 31 -- X-MS-TNEF-Correlator:
24, 31 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation
24, 31 -- thread-index: Acnfn4IHGwgMKj7FQGGCWpxtbb0YEgAAf6cA
24, 31 -- References: {200905281420.n4SEKo3Q011265-at-ns.microscopy.com}
24, 31 -- From: "Simpson, Derek" {Derek.Simpson-at-fei.com}
24, 31 -- To: {Microscopy-at-microscopy.com}
24, 31 -- Cc: {colijn.1-at-osu.edu}
24, 31 -- X-OriginalArrivalTime: 29 May 2009 12:49:00.0990 (UTC) FILETIME=[D6F695E0:01C9E05B]
24, 31 -- Content-Transfer-Encoding: 8bit
24, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TCn3L9005875
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 29 May 2009 10:00:28 -0500
Subject: [Microscopy] Inverted Columns are great....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues;

As Henk and others have alluded to there have been "inverted" and "tilted"
columns for years. The highest resolution scanning tranmsision
electron microsopes in the world (previously made by VG and now by NION)
were all configured with the gun at the bottom. Although the new
generation of aberation corrected are giving these now 20 year
old instruments a run for their money in the area of image resolution.

My particuliar instrument the ANL AAEM/VG HB603Z which has its FEG at the
bottom had been running since the early 90's. It is both a STEM
and TEM and has a transmission screen at the top ~ 3 meters
off the ground. Transmission screen is viewed using both TV and CCD cameras
(no need to stand on your head).

Haven't yet found any conventional geometry (i.e. gun at the top)
that can beat the microanalytical sensitivity of this design. But
I will freely admit operating in standard TEM mode isn't as convenient
as a machine primarily designed for that function. My
603Z was never intended to be a TEM, but rather an AEM.

The ANL 603Z is in the process of being decommissioned so for those
of you that want to have a look at this geometry
(while it is being slowly decommisssioned) here are 2 links.


http://tpm.amc.anl.gov
Live streaming video of the AAEM Lab
Select Macroscope & Video Source #1
Use Firefox or Safari WWW browsers

http://www.amc.anl.gov/docs/anl/AAEM/AAEMDocument.html

An old static document with some photos.


Nestor

Your Friendly Neighborhood SysOp

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 630-252-7901, Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec-at-ANL
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Visiting Professor of Physics - Northern Illinois University




===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
23, 14 -- From zaluzec-at-aaem.amc.anl.gov Fri May 29 10:00:28 2009
23, 14 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
23, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TF0SQi027169
23, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:00:28 -0500
23, 14 -- Received: from [146.139.72.108] (aem108.amc.anl.gov [146.139.72.108])
23, 14 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id n4TF0NJg026196
23, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:00:23 -0500
23, 14 -- Mime-Version: 1.0
23, 14 -- Message-Id: {p06240800c6459d003455-at-[146.139.72.108]}
23, 14 -- Date: Fri, 29 May 2009 10:00:22 -0500
23, 14 -- To: Microscopy-at-microscopy.com
23, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
23, 14 -- Subject: Inverted Columns are great....
23, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Fri, 29 May 2009 10:15:10 -0500
Subject: [Microscopy] Inverted columns and N2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Everyone

The past week has been one of the most enjoyable and informative periods
I have ever seen on the server. In particular, note the discussions on
inverted columns and N2. The threads have been informative, but at the
same time there has been a bit of friendly humour - lighthearted banter.

I would like to thank the list, and in particular those who contributed
to these two threads. It has been great.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009
7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFF96Y006040
7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:15:09 -0500
7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
7, 18 -- (authenticated bits=0)
7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n4TFF57l019281;
7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT)
7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca}
7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500
7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
7, 18 -- MIME-Version: 1.0
7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
7, 18 -- Subject: Inverted columns and N2
7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 18 -- Content-Transfer-Encoding: 7bit
7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 29 May 2009 10:22:41 -0500
Subject: [Microscopy] Inverted columns and N2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Agreed, Paul. And as an aside, I would like to emphasize that ALL
column orientations are welcome in our field. Electron microscopy has a
tent big enough for all.

Cheers,
Randy

-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Friday, May 29, 2009 10:16 AM
To: Tindall, Randy D.

Everyone

The past week has been one of the most enjoyable and informative periods

I have ever seen on the server. In particular, note the discussions on
inverted columns and N2. The threads have been informative, but at the
same time there has been a bit of friendly humour - lighthearted
banter.

I would like to thank the list, and in particular those who contributed
to these two threads. It has been great.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original
Headers==============================
7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009
7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca
[130.179.16.34])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n4TFF96Y006040
7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
10:15:09 -0500
7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca
[140.193.25.69])
7, 18 -- (authenticated bits=0)
7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id
n4TFF57l019281;
7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT)
7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca}
7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500
7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
7, 18 -- MIME-Version: 1.0
7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
7, 18 -- Subject: Inverted columns and N2
7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 18 -- Content-Transfer-Encoding: 7bit
7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist
==============================End of -
Headers==============================


==============================Original Headers==============================
16, 30 -- From TindallR-at-missouri.edu Fri May 29 10:22:41 2009
16, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
16, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFMeNK023300
16, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:22:41 -0500
16, 30 -- X-IronPort-Anti-Spam-Filtered: true
16, 30 -- X-IronPort-Anti-Spam-Result: ApoEADuaH0rRauUp/2dsb2JhbADDGwEJhk6IUQKCVYE1BQ
16, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
16, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 29 May 2009 10:22:40 -0500
16, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
16, 30 -- Fri, 29 May 2009 10:22:39 -0500
16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 30 -- Content-class: urn:content-classes:message
16, 30 -- MIME-Version: 1.0
16, 30 -- Content-Type: text/plain;
16, 30 -- charset="us-ascii"
16, 30 -- Subject: RE: [Microscopy] Inverted columns and N2
16, 30 -- Date: Fri, 29 May 2009 10:22:32 -0500
16, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8134-at-UM-XMAIL08.um.umsystem.edu}
16, 30 -- In-Reply-To: {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
16, 30 -- X-MS-Has-Attach:
16, 30 -- X-MS-TNEF-Correlator:
16, 30 -- Thread-Topic: [Microscopy] Inverted columns and N2
16, 30 -- Thread-Index: AcngcG7DYbYyR9o3QJCZQ5XTQYFPsAAAJcdA
16, 30 -- References: {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
16, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
16, 30 -- To: {paul_hazelton-at-umanitoba.ca}
16, 30 -- Cc: {microscopy-at-microscopy.com}
16, 30 -- X-OriginalArrivalTime: 29 May 2009 15:22:39.0904 (UTC) FILETIME=[4DDD3A00:01C9E071]
16, 30 -- Content-Transfer-Encoding: 8bit
16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TFMeNK023300
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Fri, 29 May 2009 10:30:01 -0500
Subject: [Microscopy] RE: Inverted columns and N2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopists,
Maybe now is time to consider whether inverted columns
increase or decrease the danger from nitrogen? (wink).
Tobias

}
} ----------------------------------------------------------------------------
}
} Agreed, Paul. And as an aside, I would like to emphasize that ALL
} column orientations are welcome in our field. Electron microscopy has a
} tent big enough for all.
}
} Cheers,
} Randy
}
} -----Original Message-----
} X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
} Sent: Friday, May 29, 2009 10:16 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Inverted columns and N2
}
}



} Q.html
} ------------------------------------------------------------------------
} ----
}
} Everyone
}
} The past week has been one of the most enjoyable and informative periods
}
} I have ever seen on the server. In particular, note the discussions on
} inverted columns and N2. The threads have been informative, but at the
} same time there has been a bit of friendly humour - lighthearted
} banter.
}
} I would like to thank the list, and in particular those who contributed
} to these two threads. It has been great.
}
} paul
}
} --
} Paul R. Hazelton, PhD
} Viral Gastroenteritis Study Group
} University of Manitoba
} Department of Medical Microbiology
} 511 Basic Medical Sciences Building
} 745 William Avenue
} Winnipeg, Manitoba, Canada, R3E 0J9
} e-mail: paul_hazelton-at-umanitoba.ca
} paulhazelton-at-mts.net
} Phone: 204-789-3313 (w);
} 204-489-6924 (h)
} Cell: 204-781-6982
} Fax: 204-789-3926
}
}
}
} ==============================Original
} Headers==============================
} 7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009
} 7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca
} [130.179.16.34])
} 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4TFF96Y006040
} 7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
} 10:15:09 -0500
} 7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca
} [140.193.25.69])
} 7, 18 -- (authenticated bits=0)
} 7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id
} n4TFF57l019281;
} 7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT)
} 7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca}
} 7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500
} 7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
} 7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
} 7, 18 -- MIME-Version: 1.0
} 7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
} 7, 18 -- Subject: Inverted columns and N2
} 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 18 -- Content-Transfer-Encoding: 7bit
} 7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist
} ==============================End of -
} Headers==============================
}
}
} ==============================Original Headers==============================
} 16, 30 -- From TindallR-at-missouri.edu Fri May 29 10:22:41 2009
} 16, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 16, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4TFMeNK023300
} 16, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
} 10:22:41 -0500
} 16, 30 -- X-IronPort-Anti-Spam-Filtered: true
} 16, 30 -- X-IronPort-Anti-Spam-Result:
} ApoEADuaH0rRauUp/2dsb2JhbADDGwEJhk6IUQKCVYE1BQ
} 16, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} ([209.106.229.41])
} 16, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 29
} May 2009 10:22:40 -0500
} 16, 30 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft
} SMTPSVC(6.0.3790.3959);
} 16, 30 -- Fri, 29 May 2009 10:22:39 -0500
} 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 16, 30 -- Content-class: urn:content-classes:message
} 16, 30 -- MIME-Version: 1.0
} 16, 30 -- Content-Type: text/plain;
} 16, 30 -- charset="us-ascii"
} 16, 30 -- Subject: RE: [Microscopy] Inverted columns and N2
} 16, 30 -- Date: Fri, 29 May 2009 10:22:32 -0500
} 16, 30 -- Message-ID:
} {91108EF9255B394CBF8B7E3789814A4103CD8134-at-UM-XMAIL08.um.umsystem.edu}
} 16, 30 -- In-Reply-To: {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
} 16, 30 -- X-MS-Has-Attach:
} 16, 30 -- X-MS-TNEF-Correlator:
} 16, 30 -- Thread-Topic: [Microscopy] Inverted columns and N2
} 16, 30 -- Thread-Index: AcngcG7DYbYyR9o3QJCZQ5XTQYFPsAAAJcdA
} 16, 30 -- References: {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
} 16, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 16, 30 -- To: {paul_hazelton-at-umanitoba.ca}
} 16, 30 -- Cc: {microscopy-at-microscopy.com}
} 16, 30 -- X-OriginalArrivalTime: 29 May 2009 15:22:39.0904 (UTC)
} FILETIME=[4DDD3A00:01C9E071]
} 16, 30 -- Content-Transfer-Encoding: 8bit
} 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4TFMeNK023300
} ==============================End of - Headers==============================


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
7, 21 -- From baskin-at-bio.umass.edu Fri May 29 10:30:00 2009
7, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFU0nF005519
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:30:00 -0500
7, 21 -- Received: from [172.30.52.170] (eutopia [128.119.55.30])
7, 21 -- (authenticated bits=0)
7, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n4TFTwbW026268
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 21 -- Fri, 29 May 2009 11:29:59 -0400 (EDT)
7, 21 -- Mime-Version: 1.0
7, 21 -- Message-Id: {p06240509c645af898dba-at-[172.30.52.170]}
7, 21 -- In-Reply-To: {200905291523.n4TFN9H3024540-at-ns.microscopy.com}
7, 21 -- References: {200905291523.n4TFN9H3024540-at-ns.microscopy.com}
7, 21 -- Date: Fri, 29 May 2009 11:29:57 -0400
7, 21 -- To: TindallR-at-missouri.edu
7, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
7, 21 -- Subject: RE: Inverted columns and N2
7, 21 -- Cc: microscopy-at-microscopy.com
7, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
7, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Fri, 29 May 2009 11:29:59 -0400 (EDT)
7, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Fri, 29 May 2009 10:50:16 -0500
Subject: [Microscopy] Inverted columns and N2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There's an electron column near Stanford University that is horizontal
and about a mile long. All reports are it works just fine. It is
called SLAC.

John Mardinly
Sent from my iPhone.

On May 29, 2009, at 8:36 AM, baskin-at-bio.umass.edu wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} Microscopists,
} Maybe now is time to consider whether inverted columns
} increase or decrease the danger from nitrogen? (wink).
} Tobias
}
} }
} } ---
} } ---
} } ---
} } -------------------------------------------------------------------
} }
} } Agreed, Paul. And as an aside, I would like to emphasize that ALL
} } column orientations are welcome in our field. Electron microscopy
} } has a
} } tent big enough for all.
} }
} } Cheers,
} } Randy
} }
} } -----Original Message-----
} } X-from: paul_hazelton-at-umanitoba.ca
} } [mailto:paul_hazelton-at-umanitoba.ca]
} } Sent: Friday, May 29, 2009 10:16 AM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] Inverted columns and N2
} }
} }
}
}
}
} } Q.html
} } ---
} } ---------------------------------------------------------------------
} } ----
} }
} } Everyone
} }
} } The past week has been one of the most enjoyable and informative
} } periods
} }
} } I have ever seen on the server. In particular, note the
} } discussions on
} } inverted columns and N2. The threads have been informative, but at
} } the
} } same time there has been a bit of friendly humour - lighthearted
} } banter.
} }
} } I would like to thank the list, and in particular those who
} } contributed
} } to these two threads. It has been great.
} }
} } paul
} }
} } --
} } Paul R. Hazelton, PhD
} } Viral Gastroenteritis Study Group
} } University of Manitoba
} } Department of Medical Microbiology
} } 511 Basic Medical Sciences Building
} } 745 William Avenue
} } Winnipeg, Manitoba, Canada, R3E 0J9
} } e-mail: paul_hazelton-at-umanitoba.ca
} } paulhazelton-at-mts.net
} } Phone: 204-789-3313 (w);
} } 204-489-6924 (h)
} } Cell: 204-781-6982
} } Fax: 204-789-3926
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009
} } 7, 18 -- Received: from taygeta.cc.umanitoba.ca
} } (taygeta.cc.umanitoba.ca
} } [130.179.16.34])
} } 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n4TFF96Y006040
} } 7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
} } 10:15:09 -0500
} } 7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca
} } [140.193.25.69])
} } 7, 18 -- (authenticated bits=0)
} } 7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id
} } n4TFF57l019281;
} } 7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT)
} } 7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca}
} } 7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500
} } 7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
} } 7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
} } 7, 18 -- MIME-Version: 1.0
} } 7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
} } 7, 18 -- Subject: Inverted columns and N2
} } 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 7, 18 -- Content-Transfer-Encoding: 7bit
} } 7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist
} } ==============================End of -
} } Headers==============================
} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 30 -- From TindallR-at-missouri.edu Fri May 29 10:22:41 2009
} } 16, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} } 16, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n4TFMeNK023300
} } 16, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
} } 10:22:41 -0500
} } 16, 30 -- X-IronPort-Anti-Spam-Filtered: true
} } 16, 30 -- X-IronPort-Anti-Spam-Result:
} } ApoEADuaH0rRauUp/2dsb2JhbADDGwEJhk6IUQKCVYE1BQ
} } 16, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
} } ([209.106.229.41])
} } 16, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 29
} } May 2009 10:22:40 -0500
} } 16, 30 -- Received: from UM-XMAIL08.um.umsystem.edu
} } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft
} } SMTPSVC(6.0.3790.3959);
} } 16, 30 -- Fri, 29 May 2009 10:22:39 -0500
} } 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} } 16, 30 -- Content-class: urn:content-classes:message
} } 16, 30 -- MIME-Version: 1.0
} } 16, 30 -- Content-Type: text/plain;
} } 16, 30 -- charset="us-ascii"
} } 16, 30 -- Subject: RE: [Microscopy] Inverted columns and N2
} } 16, 30 -- Date: Fri, 29 May 2009 10:22:32 -0500
} } 16, 30 -- Message-ID:
} } {91108EF9255B394CBF8B7E3789814A4103CD8134-at-UM-XMAIL08.um.umsystem.edu}
} } 16, 30 -- In-Reply-To:
} } {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
} } 16, 30 -- X-MS-Has-Attach:
} } 16, 30 -- X-MS-TNEF-Correlator:
} } 16, 30 -- Thread-Topic: [Microscopy] Inverted columns and N2
} } 16, 30 -- Thread-Index: AcngcG7DYbYyR9o3QJCZQ5XTQYFPsAAAJcdA
} } 16, 30 -- References: {200905291516.n4TFGL0A008585-at-ns.microscopy.com}
} } 16, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} } 16, 30 -- To: {paul_hazelton-at-umanitoba.ca}
} } 16, 30 -- Cc: {microscopy-at-microscopy.com}
} } 16, 30 -- X-OriginalArrivalTime: 29 May 2009 15:22:39.0904 (UTC)
} } FILETIME=[4DDD3A00:01C9E071]
} } 16, 30 -- Content-Transfer-Encoding: 8bit
} } 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id n4TFMeNK023300
} } ==============================End of -
} } Headers==============================
}
}
} --
} _ ____ __ ____
} / \ / / \ / \ \ Tobias I. Baskin
} / / / / \ \ \ Biology Department
} /_ / __ /__ \ \ \__ 611 N. Pleasant St.
} / / / \ \ \ University of
} Massachusetts
} / / / \ \ \ Amherst, MA, 01003
} / / ___ / \ \__/ \ ____
} www.bio.umass.edu/biology/baskin
} Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
}
} ==============================Original
} Headers==============================
} 7, 21 -- From baskin-at-bio.umass.edu Fri May 29 10:30:00 2009
} 7, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu
} [128.119.55.19])
} 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n4TFU0nF005519
} 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009
} 10:30:00 -0500
} 7, 21 -- Received: from [172.30.52.170] (eutopia [128.119.55.30])
} 7, 21 -- (authenticated bits=0)
} 7, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id
} n4TFTwbW026268
} 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NO);
} 7, 21 -- Fri, 29 May 2009 11:29:59 -0400 (EDT)
} 7, 21 -- Mime-Version: 1.0
} 7, 21 -- Message-Id: {p06240509c645af898dba-at-[172.30.52.170]}
} 7, 21 -- In-Reply-To: {200905291523.n4TFN9H3024540-at-ns.microscopy.com}
} 7, 21 -- References: {200905291523.n4TFN9H3024540-at-ns.microscopy.com}
} 7, 21 -- Date: Fri, 29 May 2009 11:29:57 -0400
} 7, 21 -- To: TindallR-at-missouri.edu
} 7, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
} 7, 21 -- Subject: RE: Inverted columns and N2
} 7, 21 -- Cc: microscopy-at-microscopy.com
} 7, 21 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} 7, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by
} milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Fri, 29
} May 2009 11:29:59 -0400 (EDT)
} 7, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
4, 43 -- From jmardinly-at-gmail.com Fri May 29 10:50:16 2009
4, 43 -- Received: from mail-pz0-f196.google.com (mail-pz0-f196.google.com [209.85.222.196])
4, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFoFxe020573
4, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:50:16 -0500
4, 43 -- Received: by pzk34 with SMTP id 34so1548616pzk.10
4, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 08:50:15 -0700 (PDT)
4, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=domainkey-signature:received:received:references:message-id:from:to
4, 43 -- :in-reply-to:content-type:content-transfer-encoding:x-mailer
4, 43 -- :mime-version:subject:date:cc;
4, 43 -- bh=yWn8xGn3oEyF2y+GQScwlkIi+MkYTvA0T8VUSFw4Tb8=;
4, 43 -- b=GHVo0SdZMgfJbXkpB2C1USMmwwZ2ArhEYOovfpD7nWuLxSneSuaUZKWPBM8HlnIdb0
4, 43 -- 8b5A8+GxTjZaIo6ddcLnV+2UHGjxPQ5jqJvJq7r1XQDd5Kz1gxgiGiXt4nKWwgzE5WKa
4, 43 -- ZzYo/H5GaKKy6DMXWJe7zKbduMrgxMbOPu3GE=
4, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=references:message-id:from:to:in-reply-to:content-type
4, 43 -- :content-transfer-encoding:x-mailer:mime-version:subject:date:cc;
4, 43 -- b=OJhuwPXrNxagV4pRNDn8c93yWvxSt9o0mmUAO5wOHrtCnwhReh9X04oSaT93XOjVmy
4, 43 -- yMYEKW96pUYHYsu0ERIaD5dJsSgfoUd/A/mQA1iDQhDlHmzEVIHTnsFuRjNTGh8zn19W
4, 43 -- pEwX3lUJm/+lynJGsskyXo/IfJDmB2MX+7xuA=
4, 43 -- Received: by 10.114.159.5 with SMTP id h5mr4379049wae.101.1243612214963;
4, 43 -- Fri, 29 May 2009 08:50:14 -0700 (PDT)
4, 43 -- Received: from ?192.168.1.102? (75-25-131-32.lightspeed.sjcpca.sbcglobal.net [75.25.131.32])
4, 43 -- by mx.google.com with ESMTPS id j15sm2302184waf.16.2009.05.29.08.50.13
4, 43 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 43 -- Fri, 29 May 2009 08:50:14 -0700 (PDT)
4, 43 -- References: {200905291536.n4TFalMT019182-at-ns.microscopy.com}
4, 43 -- Message-Id: {B19FD6ED-4587-46EE-86F4-40FA3B91FDF8-at-gmail.com}
4, 43 -- From: John Mardinly {jmardinly-at-gmail.com}
4, 43 -- To: "baskin-at-bio.umass.edu" {baskin-at-bio.umass.edu}
4, 43 -- In-Reply-To: {200905291536.n4TFalMT019182-at-ns.microscopy.com}
4, 43 -- Content-Type: text/plain;
4, 43 -- charset=us-ascii;
4, 43 -- format=flowed;
4, 43 -- delsp=yes
4, 43 -- Content-Transfer-Encoding: 7bit
4, 43 -- X-Mailer: iPhone Mail (5H11)
4, 43 -- Mime-Version: 1.0 (iPhone Mail 5H11)
4, 43 -- Subject: Re: [Microscopy] RE: Inverted columns and N2
4, 43 -- Date: Fri, 29 May 2009 08:50:08 -0700
4, 43 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Fri, 29 May 2009 16:09:21 -0500
Subject: [Microscopy] Inverted Columns are great....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like everyone to sign my petition that electron microscopy be
designated as occurring in an upright column between one gun and one
transmission screen. Any other orientation or configuration should have
to be satisfied with civil union microscopy...(wink). (Sorry all, it's
Friday, and I've had to cram 5 days of work into 4, so I'm getting a
little silly)

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv. Permission is
specifically granted to the Microscopy Society of America to publish
some or all of this message in the Microscopy Today journal.

-----Original Message-----

} Microscopists,
} Maybe now is time to consider whether inverted columns
} increase or decrease the danger from nitrogen? (wink).
} Tobias
}
} }
} } ----------------------------------------------------------------------
----
} }
} } Agreed, Paul. And as an aside, I would like to emphasize that ALL
} } column orientations are welcome in our field. Electron microscopy has
a
} } tent big enough for all.
} }
} } Cheers,
} } Randy

This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
9, 34 -- From Robert.Zonis-at-Sanford.com Fri May 29 10:54:13 2009
9, 34 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243])
9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4TFsC9O027153
9, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:54:13 -0500
9, 34 -- X-VirusChecked: Checked
9, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
9, 34 -- X-Msg-Ref: server-13.tower-192.messagelabs.com!1243612445!38069439!11
9, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
9, 34 -- X-Originating-IP: [198.176.16.26]
9, 34 -- Received: (qmail 16150 invoked from network); 29 May 2009 15:54:12 -0000
9, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26)
9, 34 -- by server-13.tower-192.messagelabs.com with SMTP; 29 May 2009 15:54:12 -0000
9, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
9, 34 -- Fri, 29 May 2009 10:53:51 -0500
9, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 34 -- Content-class: urn:content-classes:message
9, 34 -- MIME-Version: 1.0
9, 34 -- Content-Type: text/plain;
9, 34 -- charset="us-ascii"
9, 34 -- Subject: RE: [Microscopy] RE: Inverted columns and N2
9, 34 -- Date: Fri, 29 May 2009 10:50:31 -0500
9, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402E72545-at-naoaksasebe02.nr.ad.newellco.com}
9, 34 -- In-Reply-To: {200905291534.n4TFYXSV014450-at-ns.microscopy.com}
9, 34 -- X-MS-Has-Attach:
9, 34 -- X-MS-TNEF-Correlator:
9, 34 -- Thread-Topic: [Microscopy] RE: Inverted columns and N2
9, 34 -- thread-index: AcngcvjDXwFgCgoQRPG31Bh2HV+6NwAANJ9g
9, 34 -- References: {200905291534.n4TFYXSV014450-at-ns.microscopy.com}
9, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
9, 34 -- To: {Microscopy-at-microscopy.com}
9, 34 -- Cc: {baskin-at-bio.umass.edu}
9, 34 -- X-OriginalArrivalTime: 29 May 2009 15:53:51.0642 (UTC) FILETIME=[A981C7A0:01C9E075]
9, 34 -- Content-Transfer-Encoding: 8bit
9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TFsC9O027153
==============================End of - Headers==============================

From bestsite103-at-hotmail.com Fri May 29 15:17:53 2009
Return-Path: {bestsite103-at-hotmail.com}
Received: from google.com (59-104-156-240.adsl.dynamic.seed.net.tw [59.104.156.240])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TKHqAX007657
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 29 May 2009 15:17:53 -0500
Received: from [119.71.63.188] (HELO google.com)
by huge-ranked.org; Sat, 30 May 2009 04:17:44 +0800
Message-ID: {0000000473483A61293987394}
Reply-To: Jerrard Joyce {monie.misty1185-at-gmail.com}

When I introduce our CM100 TEM to new users, I tell them it is configured like an inverted optical microscope. If that's the case, isn't the conventional TEM column really "inverted"???

All kidding aside, we've been calling it an "inverted column", which I still consider literally acceptable if the viewing screen or table is the baseline. Is it an actual industry standard for describing the more common TEM column arrangement "conventional" or "upright?" Is "inverted" also an acceptable industry-wide description? (It didn't seem to confuse anyone on this conversation.)

Regards,
~Gregg
Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Friday, May 29, 2009 11:17 AM
To: Sobocinski, Gregg

Colleagues;

As Henk and others have alluded to there have been "inverted" and "tilted"
columns for years. The highest resolution scanning transmission
electron microscopes in the world (previously made by VG and now by NION)
were all configured with the gun at the bottom. Although the new
generation of aberration corrected are giving these now 20 year
old instruments a run for their money in the area of image resolution.

My particuliar instrument the ANL AAEM/VG HB603Z which has its FEG at the
bottom had been running since the early 90's. It is both a STEM
and TEM and has a transmission screen at the top ~ 3 meters
off the ground. Transmission screen is viewed using both TV and CCD cameras
(no need to stand on your head).

Haven't yet found any conventional geometry (i.e. gun at the top)
that can beat the microanalytical sensitivity of this design. But
I will freely admit operating in standard TEM mode isn't as convenient
as a machine primarily designed for that function. My
603Z was never intended to be a TEM, but rather an AEM.

The ANL 603Z is in the process of being decommissioned so for those
of you that want to have a look at this geometry
(while it is being slowly decommisssioned) here are 2 links.


http://tpm.amc.anl.gov
Live streaming video of the AAEM Lab
Select Macroscope & Video Source #1
Use Firefox or Safari WWW browsers

http://www.amc.anl.gov/docs/anl/AAEM/AAEMDocument.html

An old static document with some photos.


Nestor

Your Friendly Neighborhood SysOp

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 630-252-7901, Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec-at-ANL
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Visiting Professor of Physics - Northern Illinois University




===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
23, 14 -- From zaluzec-at-aaem.amc.anl.gov Fri May 29 10:00:28 2009
23, 14 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
23, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TF0SQi027169
23, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:00:28 -0500
23, 14 -- Received: from [146.139.72.108] (aem108.amc.anl.gov [146.139.72.108])
23, 14 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id n4TF0NJg026196
23, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:00:23 -0500
23, 14 -- Mime-Version: 1.0
23, 14 -- Message-Id: {p06240800c6459d003455-at-[146.139.72.108]}
23, 14 -- Date: Fri, 29 May 2009 10:00:22 -0500
23, 14 -- To: Microscopy-at-microscopy.com
23, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
23, 14 -- Subject: Inverted Columns are great....
23, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
32, 25 -- From greggps-at-umich.edu Fri May 29 16:09:21 2009
32, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
32, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TL9LWA015877
32, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 16:09:21 -0500
32, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.234]) by
32, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Fri, 29 May
32, 25 -- 2009 17:09:21 -0400
32, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
32, 25 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
32, 25 -- Date: Fri, 29 May 2009 17:09:11 -0400
32, 25 -- Subject: RE: [Microscopy] Inverted Columns: Are they really inverted?
32, 25 -- Thread-Topic: [Microscopy] Inverted Columns: Are they really inverted?
32, 25 -- Thread-Index: AcngcHyDI/1kzC8tTBir+w7Abz/o8gAKf87A
32, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA39340611B36EF1-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
32, 25 -- References: {200905291516.n4TFGjZi009530-at-ns.microscopy.com}
32, 25 -- In-Reply-To: {200905291516.n4TFGjZi009530-at-ns.microscopy.com}
32, 25 -- Accept-Language: en-US
32, 25 -- Content-Language: en-US
32, 25 -- X-MS-Has-Attach:
32, 25 -- X-MS-TNEF-Correlator:
32, 25 -- acceptlanguage: en-US
32, 25 -- Content-Type: text/plain; charset="us-ascii"
32, 25 -- MIME-Version: 1.0
32, 25 -- Content-Transfer-Encoding: 8bit
32, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TL9LWA015877
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Fri, 29 May 2009 17:21:52 -0500
Subject: [Microscopy] viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.r.thorpe-at-sussex.ac.uk as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.r.thorpe-at-sussex.ac.uk
Name: Julian Thorpe

Organization: University of Sussex

Title-Subject: [Filtered] TEM Sample Preparation Charges

Question: Dear All,
By the way, as introduction I should say that I'm
in charge of the EM part of the Sussex Centre for
Advanced Microscopy here in Life Sciences at the
University of Sussex in Brighton UK.
Anyway, I've recently had the supervisor of a
postgrad student I've been helping (to prepare
leaf tissues treated in various ways to examine
effects upon chloroplast morphology) query my
charging and suggesting it was overpriced.
Here, we charge £10 per sample (if processed by
standard double-fixation approaches, dehydration
and embedding in TAAB low viscosity resin). And
by 'per sample', I mean as many pieces of tissue
(e.g. replicates of a given treatment) that can
be processed through in one glass vial.
I thought this was entirely reasonable.
Can you folks out there give me some examples of
your pricing for similar preparations, if you're
happy disclosing that of course! Otherwise, just
some general feedback would be useful.
Thanks in advance for your help,
Jules

Dr. Julian R. Thorpe
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm

Login Host: 139.184.30.134
---------------------------------------------------------------------------


==============================Original Headers==============================
9, 13 -- From zaluzec-at-microscopy.com Fri May 29 17:21:52 2009
9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TMLpoX032550
9, 13 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 17:21:52 -0500
9, 13 -- Mime-Version: 1.0
9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]}
9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500
9, 13 -- To: microscopy-at-microscopy.com
9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges
9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 13 -- Content-Transfer-Encoding: 8bit
9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TMLpoX032550
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Sat, 30 May 2009 07:55:53 -0500
Subject: [Microscopy] Re: viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Julian,


I'm not in biology or medicine but, I still think that you should
charge more than what you are currently charging. Because, you are
using your expertise. If the other party thinks your pricing is high
then he/she should obtain the expertise and prepare his samples by
himself.


Sorry, I jumped in even though it is not my field of expertise.
Because, it touched a nerve. I come across researchers who thinks that
TEM work is not a scientific contribution even when used in a research
paper.


At the moment, I'm doing TEM investigation for all the researchers. We
do not have enough trained people and we do not have the system in
place where I would give basic TEM training to a new user and leave
him on his own. Hopefully, we will have this system later on.


Recently, I had an inquiry that one researcher wants me to find out
size distribution of metal nano-particles which are 0.4 - 1.0 nm in
size based on XRD study. It sounds a lot hard to find them in the
first place. So, I told him that I can undertake this study only if I
will be included in the paper as co-author. He did not agree and said
that "You will give me two images. What is the scientific contribution
here?"


Regards,
Ayten.

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================



On Sat, May 30, 2009 at 1:27 AM, {j.r.thorpe-at-sussex.ac.uk} wrote:
}
}
} Email: j.r.thorpe-at-sussex.ac.uk
} Name: Julian Thorpe
}
} Organization: University of Sussex
}
} Title-Subject: [Filtered] TEM Sample Preparation Charges
}
} Question: Dear All,
} By the way, as introduction I should say that I'm
} in charge of the EM part of the Sussex Centre for
} Advanced Microscopy here in Life Sciences at the
} University of Sussex in Brighton UK.
} Anyway, I've recently had the supervisor of a
} postgrad student I've been helping (to prepare
} leaf tissues treated in various ways to examine
} effects upon chloroplast morphology) query my
} charging and suggesting it was overpriced.
} Here, we charge £10 per sample (if processed by
} standard double-fixation approaches, dehydration
} and embedding in TAAB low viscosity resin). And
} by 'per sample', I mean as many pieces of tissue
} (e.g. replicates of a given treatment) that can
} be processed through in one glass vial.
} I thought this was entirely reasonable.
} Can you folks out there give me some examples of
} your pricing for similar preparations, if you're
} happy disclosing that of course! Otherwise, just
} some general feedback would be useful.
} Thanks in advance for your help,
} Jules
}
} Dr. Julian R. Thorpe
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
} Tel.: ext +44 (0)1273 877585
}       int 7585
}
} URLs:
} (home)
} http://www.sussex.ac.uk/biology/profile2686.html
} (lab)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} (research)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
}
}  Login Host: 139.184.30.134
} ---------------------------------------------------------------------------
}
}
} ==============================Original Headers==============================
} 9, 13 -- From zaluzec-at-microscopy.com Fri May 29 17:21:52 2009
} 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 9, 13 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TMLpoX032550
} 9, 13 --        for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 17:21:52 -0500
} 9, 13 -- Mime-Version: 1.0
} 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]}
} 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500
} 9, 13 -- To: microscopy-at-microscopy.com
} 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
} 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges
} 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} 9, 13 -- Content-Transfer-Encoding: 8bit
} 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TMLpoX032550
} ==============================End of - Headers==============================


==============================Original Headers==============================
16, 36 -- From celikaktas-at-gmail.com Sat May 30 07:55:53 2009
16, 36 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4UCtqxm026348
16, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 07:55:53 -0500
16, 36 -- Received: by fxm8 with SMTP id 8so7876581fxm.18
16, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 05:55:52 -0700 (PDT)
16, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 36 -- d=gmail.com; s=gamma;
16, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references
16, 36 -- :date:message-id:subject:from:to:cc:content-type
16, 36 -- :content-transfer-encoding;
16, 36 -- bh=c+7cZgjMLGWY2e4EBUnaCifFlQ/h5JNNbTr314T+gyU=;
16, 36 -- b=lyWnxbyvNc5XclMvVmbs3gZbpJ2dFZbp039knhWliNdaeGXBykWNiVAKOEnP4j7qYv
16, 36 -- tPZ/L2immyG65ujQHnBurhE/ou2woRNGCey0K0ZtIPaHAzJIbC//tMx1hstw53z5Xxey
16, 36 -- T3mH+wrWPEtOwByznd61HhQgg9M/g010wIJtU=
16, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
16, 36 -- d=gmail.com; s=gamma;
16, 36 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
16, 36 -- :cc:content-type:content-transfer-encoding;
16, 36 -- b=q4xT1TLGUijMOHUHftLA6Mk4Xg1228xBun0obP6dFPJtePMlGl093Z+Afm3XpMACDD
16, 36 -- Jpr373wfE8nodF6snhBwhv4wtved3cT7GMsAD2fp45JcaZmZI5QLjIDdB/rQ8Nd5io6d
16, 36 -- ZqWXu2FSKPQMX+O9CEhlX05PVgrpezSFx9B00=
16, 36 -- MIME-Version: 1.0
16, 36 -- Received: by 10.103.223.2 with SMTP id a2mr2249133mur.4.1243688152197; Sat, 30
16, 36 -- May 2009 05:55:52 -0700 (PDT)
16, 36 -- In-Reply-To: {200905292227.n4TMRolY010992-at-ns.microscopy.com}
16, 36 -- References: {200905292227.n4TMRolY010992-at-ns.microscopy.com}
16, 36 -- Date: Sat, 30 May 2009 15:55:52 +0300
16, 36 -- Message-ID: {1075c5c10905300555m3efb167ek7c0d77482e9ba55e-at-mail.gmail.com}
16, 36 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges
16, 36 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
16, 36 -- To: j.r.thorpe-at-sussex.ac.uk
16, 36 -- Cc: microscopy {Microscopy-at-microscopy.com}
16, 36 -- Content-Type: text/plain; charset=UTF-8
16, 36 -- Content-Transfer-Encoding: 8bit
16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4UCtqxm026348
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Sat, 30 May 2009 17:34:49 -0500
Subject: [Microscopy] Re: viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Julian-

Due to the extremely wide variety of samples we are asked to process, we
do not have a flat rate, but charge for chemicals and for technician time
by the hour. We made up an informative sort of spreadsheet that helps
prospective clients see how long it will take (sometimes the most
important part when they want TEM!) and where the charges are, so that
they can decide if they want to do any, part, or all of it themselves.

Here's the bottom line for fixation to a few images on the TEM: $411 to
$645 (USD) for the first sample, plus another couple hundred dollars EACH
for more samples done at the same time! About $250 - $450 just to get them
into blocks, primarily because we charge $43/hour for tech time. It is
USUALLY much cheaper than this because they do not have to pay for me to
stand at the fume hood doing nothing while waiting to change to the next
alcohol, BUT they need to be prepared for this, if necessary.

So show them this and tell them they are getting a real bargain.

Aloha,
Tina

} Question: Dear All,
} By the way, as introduction I should say that I'm
} in charge of the EM part of the Sussex Centre for
} Advanced Microscopy here in Life Sciences at the
} University of Sussex in Brighton UK.
} Anyway, I've recently had the supervisor of a
} postgrad student I've been helping (to prepare
} leaf tissues treated in various ways to examine
} effects upon chloroplast morphology) query my
} charging and suggesting it was overpriced.
} Here, we charge £10 per sample (if processed by
} standard double-fixation approaches, dehydration
} and embedding in TAAB low viscosity resin). And
} by 'per sample', I mean as many pieces of tissue
} (e.g. replicates of a given treatment) that can
} be processed through in one glass vial.
} I thought this was entirely reasonable.
} Can you folks out there give me some examples of
} your pricing for similar preparations, if you're
} happy disclosing that of course! Otherwise, just
} some general feedback would be useful.
} Thanks in advance for your help,
} Jules
}
} Dr. Julian R. Thorpe
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
} Tel.: ext +44 (0)1273 877585
} int 7585
}
} URLs:
} (home)
} http://www.sussex.ac.uk/biology/profile2686.html
} (lab)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} (research)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
}
} Login Host: 139.184.30.134
} ---------------------------------------------------------------------------
}
}
} ==============================Original Headers==============================
} 9, 13 -- From zaluzec-at-microscopy.com Fri May 29 17:21:52 2009
} 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TMLpoX032550
} 9, 13 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 17:21:52 -0500
} 9, 13 -- Mime-Version: 1.0
} 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]}
} 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500
} 9, 13 -- To: microscopy-at-microscopy.com
} 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
} 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges
} 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} 9, 13 -- Content-Transfer-Encoding: 8bit
} 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TMLpoX032550
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



==============================Original Headers==============================
9, 23 -- From tina-at-pbrc.hawaii.edu Sat May 30 17:34:49 2009
9, 23 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4UMYmuD010985
9, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 17:34:48 -0500
9, 23 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
9, 23 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n4UMYh6Y007963
9, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
9, 23 -- Sat, 30 May 2009 12:34:44 -1000 (HST)
9, 23 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n4UMYfCo007960;
9, 23 -- Sat, 30 May 2009 12:34:42 -1000 (HST)
9, 23 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
9, 23 -- Date: Sat, 30 May 2009 12:34:40 -1000 (HST)
9, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
9, 23 -- X-Sender: tina-at-halia
9, 23 -- To: j.r.thorpe-at-sussex.ac.uk
9, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 23 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges
9, 23 -- In-Reply-To: {200905292222.n4TMMux0001634-at-ns.microscopy.com}
9, 23 -- Message-ID: {Pine.GSO.4.21.0905301221460.7927-100000-at-halia}
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: TEXT/PLAIN; charset=X-UNKNOWN
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n4UMYmuD010985
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Mon, 1 Jun 2009 04:25:01 -0500
Subject: [Microscopy] Re: viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bradford, Derrick, Paula, Teresa, Dale, Shashi, Ayten, Jim, Tina, John, Rosemary and anyone else I may have left out!

Many thanks for all your feedback on my post. Very useful, thank you, and I
feel more vindicated now that I am certainly not overcharging for my TEM preparation!

Cheers,
Jules


On 29-May-09, at 3:25 PM, j.r.thorpe-at-sussex.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both j.r.thorpe-at-sussex.ac.uk as
} well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: j.r.thorpe-at-sussex.ac.uk
} Name: Julian Thorpe
}
} Organization: University of Sussex
}
} Title-Subject: [Filtered] TEM Sample Preparation Charges
}
} Question: Dear All,
} By the way, as introduction I should say that I'm
} in charge of the EM part of the Sussex Centre for
} Advanced Microscopy here in Life Sciences at the
} University of Sussex in Brighton UK.
} Anyway, I've recently had the supervisor of a
} postgrad student I've been helping (to prepare
} leaf tissues treated in various ways to examine
} effects upon chloroplast morphology) query my
} charging and suggesting it was overpriced.
} Here, we charge £10 per sample (if processed by
} standard double-fixation approaches, dehydration
} and embedding in TAAB low viscosity resin). And
} by 'per sample', I mean as many pieces of tissue
} (e.g. replicates of a given treatment) that can
} be processed through in one glass vial.
} I thought this was entirely reasonable.
} Can you folks out there give me some examples of
} your pricing for similar preparations, if you're
} happy disclosing that of course! Otherwise, just
} some general feedback would be useful.
} Thanks in advance for your help,
} Jules
}
} Dr. Julian R. Thorpe
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
} Tel.: ext +44 (0)1273 877585
} int 7585
}
} URLs:
} (home)
} http://www.sussex.ac.uk/biology/profile2686.html
} (lab)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} (research)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm



==============================Original Headers==============================
7, 24 -- From bafg3-at-sussex.ac.uk Mon Jun 1 04:24:59 2009
7, 24 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n519OuY1002821
7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 04:24:58 -0500
7, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:2040)
7, 24 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
7, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} )
7, 24 -- id KKJZI9-00044S-HL
7, 24 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 10:25:21 +0100
7, 24 -- Date: Mon, 01 Jun 2009 10:21:34 +0100
7, 24 -- To: Microscopy-at-microscopy.com
7, 24 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges
7, 24 -- Message-ID: {2606146295.1243851694-at-ls0130.lifesci.susx.ac.uk}
7, 24 -- Originator-Info: login-token=Mulberry:01TqCG1rAB7D2C4OYwtwR8E/tJwgXx/a/7fBXx;
7, 24 -- token_authority=postmaster-at-central.susx.ac.uk
7, 24 -- X-Mailer: Mulberry/2.0.8 (Win32)
7, 24 -- MIME-Version: 1.0
7, 24 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed
7, 24 -- Content-Disposition: inline
7, 24 -- X-Sussex: true
7, 24 -- X-Sussex-transport: remote_smtp_rew
7, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
7, 24 -- Content-Transfer-Encoding: 8bit
7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n519OuY1002821
==============================End of - Headers==============================




From: marjolein.snippe-at-csc.mrc.ac.uk
Date: Mon, 1 Jun 2009 10:26:43 -0500
Subject: [Microscopy] EM Tokuyasu preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We have received some post-mortem FROZEN human brain pieces which we
would like to aldehyde fix and embed in sucrose for Tokuyasu
cryosectioning. Could anyone recommend the best fixative and fixation
procedure?

Would it work to just transfer the frozen tissue samples (0.5cm wide) to
melt in a buffered formaldehyde solution, possibly in the presence of
sucrose or another cryoprotectant? Would it be best to have the fixative
at 4oC for slow melting or at room temperature?

Thanks in advance,
Ana and Sheila


==============================Original Headers==============================
5, 19 -- From marjolein.snippe-at-csc.mrc.ac.uk Mon Jun 1 10:26:43 2009
5, 19 -- Received: from smtp1.cc.ic.ac.uk (smtp1.cc.ic.ac.uk [155.198.5.155])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n51FQhil031720
5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 10:26:43 -0500
5, 19 -- Received: from default.csc.mrc.ac.uk ([193.60.222.2] helo=[10.23.5.51])
5, 19 -- by smtp1.cc.ic.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
5, 19 -- (Exim 4.69)
5, 19 -- (envelope-from {marjolein.snippe-at-csc.mrc.ac.uk} )
5, 19 -- id 1MB9PO-0005uQ-LR
5, 19 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 16:26:42 +0100
5, 19 -- Message-ID: {4A23F332.7020908-at-imperial.ac.uk}
5, 19 -- Date: Mon, 01 Jun 2009 16:26:42 +0100
5, 19 -- From: Marjolein Snippe {marjolein.snippe-at-csc.mrc.ac.uk}
5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
5, 19 -- MIME-Version: 1.0
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- Subject: EM Tokuyasu preparation
5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Mon, 1 Jun 2009 11:05:02 -0500
Subject: [Microscopy] RE: viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks Randy. The truth is that these charges will definitely go up once people with research funds (hopefully!) successfully acquired for projects that have been 'fully economically costed', FEC as we say here, come through the system. At present I am just about able to cover my service contracts on my TEM and the associated camera with the income generated in my lab.
Jules

--On 01 June 2009 08:33 -0500 "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:

} I agree with the others. Your service is extremely cheap. We couldn't
} begin to cover the costs of our service with fees like you charge.
}
} We occasionally get complaints about price (normally from researchers
} from other countries for some reason), but I just calmly explain the
} steps we go through and show them some corporate rates for EM and they
} usually quiet down.
}
} Good luck.
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=W
} eek&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
}
}
}
} -----Original Message-----
} From: j.r.thorpe-at-sussex.ac.uk [mailto:j.r.thorpe-at-sussex.ac.uk]
} Sent: Friday, May 29, 2009 5:23 PM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: TEM Sample Preparation Charges
}
}
}
}
} -------------------------------------------------------------------------
} --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------
} ---
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} -------------------------------------------------------------------------
} -- Remember this posting is most likely not from a Subscriber, so when
} replying please copy both j.r.thorpe-at-sussex.ac.uk as
} well as the MIcroscopy Listserver
} -------------------------------------------------------------------------
} --
}
} Email: j.r.thorpe-at-sussex.ac.uk
} Name: Julian Thorpe
}
} Organization: University of Sussex
}
} Title-Subject: [Filtered] TEM Sample Preparation Charges
}
} Question: Dear All,
} By the way, as introduction I should say that I'm
} in charge of the EM part of the Sussex Centre for
} Advanced Microscopy here in Life Sciences at the
} University of Sussex in Brighton UK.
} Anyway, I've recently had the supervisor of a
} postgrad student I've been helping (to prepare
} leaf tissues treated in various ways to examine
} effects upon chloroplast morphology) query my
} charging and suggesting it was overpriced.
} Here, we charge £10 per sample (if processed by
} standard double-fixation approaches, dehydration
} and embedding in TAAB low viscosity resin). And
} by 'per sample', I mean as many pieces of tissue
} (e.g. replicates of a given treatment) that can
} be processed through in one glass vial.
} I thought this was entirely reasonable.
} Can you folks out there give me some examples of
} your pricing for similar preparations, if you're
} happy disclosing that of course! Otherwise, just
} some general feedback would be useful.
} Thanks in advance for your help,
} Jules
}
} Dr. Julian R. Thorpe
} Electron Microscope Division,
} The Sussex Centre for Advanced Microscopy,
} John Maynard-Smith Building,
} School of Life Sciences,
} University of Sussex,
} Falmer,
} Brighton BN1 9QG
} Tel.: ext +44 (0)1273 877585
} int 7585
}
} URLs:
} (home)
} http://www.sussex.ac.uk/biology/profile2686.html
} (lab)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} (research)
} http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
}
} Login Host: 139.184.30.134
} -------------------------------------------------------------------------
} --
}
}
} ==============================Original
} Headers============================== 9, 13 -- From
} zaluzec-at-microscopy.com Fri May 29 17:21:52 2009
} 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n4TMLpoX032550 9, 13 -- for {microscopy-at-microscopy.com} ;
} Fri, 29 May 2009 17:21:52 -0500 9, 13 -- Mime-Version: 1.0
} 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]}
} 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500
} 9, 13 -- To: microscopy-at-microscopy.com
} 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
} 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges
} 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} 9, 13 -- Content-Transfer-Encoding: 8bit
} 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n4TMLpoX032550 ==============================End of
} - Headers==============================



Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm


==============================Original Headers==============================
8, 25 -- From bafg3-at-sussex.ac.uk Mon Jun 1 11:05:02 2009
8, 25 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n51G519K014832
8, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 11:05:02 -0500
8, 25 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:1179)
8, 25 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
8, 25 -- (envelope-from {bafg3-at-sussex.ac.uk} )
8, 25 -- id KKKI17-000A7R-B4
8, 25 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 17:05:31 +0100
8, 25 -- Date: Mon, 01 Jun 2009 17:05:00 +0100
8, 25 -- To: Microscopy-at-microscopy.com
8, 25 -- Subject: RE: [Microscopy] viaWWW: TEM Sample Preparation Charges
8, 25 -- Message-ID: {2630352222.1243875900-at-ls0130.lifesci.susx.ac.uk}
8, 25 -- In-Reply-To: {91108EF9255B394CBF8B7E3789814A4103CD813B-at-UM-XMAIL08.um.umsystem.edu}
8, 25 -- Originator-Info: login-token=Mulberry:01MstRopQstwlmC7H8my9gPsYVpjC8yZMmYEC8;
8, 25 -- token_authority=postmaster-at-central.susx.ac.uk
8, 25 -- X-Mailer: Mulberry/2.0.8 (Win32)
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed
8, 25 -- Content-Disposition: inline
8, 25 -- X-Sussex: true
8, 25 -- X-Sussex-transport: remote_smtp_rew
8, 25 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n51G519K014832
==============================End of - Headers==============================




From: j.r.thorpe-at-sussex.ac.uk
Date: Mon, 1 Jun 2009 11:06:29 -0500
Subject: [Microscopy] Re: viaWWW: TEM Sample Preparation Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee, thanks for that and please see my reply to Randy......
Jules

--On 01 June 2009 09:45 -0400 Leona Cohen-Gould {lcgould-at-med.cornell.edu} wrote:

} Dear Jules-
} Even with the exchange rate what it is, I can tell you that he's getting
} a bargain. My standard fee for processing, embedding, sectioning and
} contrasting the grids (in LX112, EMbed 812 or Spurr's) is $125/sample,
} with my definition of "sample" matching yours. Preps for immuno are
} billed at $200/sample, plus labelling fees. You will always encounter
} people who squawk at your pricing...I have people here who think that
} because we are a College sponsored Core Facility they should not have to
} pay anything at all! I don't know where they think the funds for things
} like salaries, materials, chemicals, equipment, equipment maintenance,
} etc come from! Good luck,
} Lee
}
} } ------------------------------------------------------------------------
} } ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } ----
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} } ------------------------------------------------------------------------
} } --- Remember this posting is most likely not from a Subscriber, so when
} } replying please copy both j.r.thorpe-at-sussex.ac.uk as
} } well as the MIcroscopy Listserver
} } ------------------------------------------------------------------------
} } ---
} }
} } Email: j.r.thorpe-at-sussex.ac.uk
} } Name: Julian Thorpe
} }
} } Organization: University of Sussex
} }
} } Title-Subject: [Filtered] TEM Sample Preparation Charges
} }
} } Question: Dear All,
} } By the way, as introduction I should say that I'm
} } in charge of the EM part of the Sussex Centre for
} } Advanced Microscopy here in Life Sciences at the
} } University of Sussex in Brighton UK.
} } Anyway, I've recently had the supervisor of a
} } postgrad student I've been helping (to prepare
} } leaf tissues treated in various ways to examine
} } effects upon chloroplast morphology) query my
} } charging and suggesting it was overpriced.
} } Here, we charge £10 per sample (if processed by
} } standard double-fixation approaches, dehydration
} } and embedding in TAAB low viscosity resin). And
} } by 'per sample', I mean as many pieces of tissue
} } (e.g. replicates of a given treatment) that can
} } be processed through in one glass vial.
} } I thought this was entirely reasonable.
} } Can you folks out there give me some examples of
} } your pricing for similar preparations, if you're
} } happy disclosing that of course! Otherwise, just
} } some general feedback would be useful.
} } Thanks in advance for your help,
} } Jules
} }
} } Dr. Julian R. Thorpe
} } Electron Microscope Division,
} } The Sussex Centre for Advanced Microscopy,
} } John Maynard-Smith Building,
} } School of Life Sciences,
} } University of Sussex,
} } Falmer,
} } Brighton BN1 9QG
} } Tel.: ext +44 (0)1273 877585
} } int 7585
} }
} } URLs:
} } (home)
} } http://www.sussex.ac.uk/biology/profile2686.html
} } (lab)
} } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
} } (research)
} } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
} }
} } Login Host: 139.184.30.134
} } ------------------------------------------------------------------------
} } ---
} }
} }
} } ==============================Original
} } Headers============================== 9, 13 -- From
} } zaluzec-at-microscopy.com Fri May 29 17:21:52 2009
} } 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22]) 9, 13 -- by ns.microscopy.com
} } (8.12.11.20060308/8.12.8) with ESMTP id
} } n4TMLpoX032550
} } 9, 13 -- for {microscopy-at-microscopy.com} ;
} } Fri, 29 May 2009 17:21:52 -0500
} } 9, 13 -- Mime-Version: 1.0
} } 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]}
} } 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500
} } 9, 13 -- To: microscopy-at-microscopy.com
} } 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver)
} } 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges
} } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} } 9, 13 -- Content-Transfer-Encoding: 8bit
} } 9, 13 -- X-MIME-Autoconverted: from
} } quoted-printable to 8bit by ns.microscopy.com id
} } n4TMLpoX032550
} } ==============================End of -
} } Headers==============================
}
}
} --
} Lee Cohen-Gould, M.S.
} Sr. Staff Associate in Biochemistry and
} Cell & Developmental Biology
} Director, Electron Microscopy & Histology
} and Optical Microscopy Core Facilities
} Weill Cornell Medical College
}
} voice (212)746-6146
} fax (212)746-8175
} http://www.med.cornell.edu/research/rea_sup/
} http://www.cornellcelldevbiology.org
} http://www.cornellbiochem.org



Dr. Julian R. Thorpe
(Office 2C9/Lab 2C11-13)
Electron Microscope Division,
The Sussex Centre for Advanced Microscopy,
John Maynard-Smith Building,
School of Life Sciences,
University of Sussex,
Falmer,
Brighton BN1 9QG
Tel.: ext +44 (0)1273 877585
int 7585

URLs:
(home)
http://www.sussex.ac.uk/biology/profile2686.html
(lab)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm
(research)
http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm


==============================Original Headers==============================
8, 25 -- From bafg3-at-sussex.ac.uk Mon Jun 1 11:06:29 2009
8, 25 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n51G6SCQ016773
8, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 11:06:28 -0500
8, 25 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:1188)
8, 25 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64)
8, 25 -- (envelope-from {bafg3-at-sussex.ac.uk} )
8, 25 -- id KKKI3L-000AJY-P3
8, 25 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 17:06:58 +0100
8, 25 -- Date: Mon, 01 Jun 2009 17:06:27 +0100
8, 25 -- To: Microscopy-at-microscopy.com
8, 25 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges
8, 25 -- Message-ID: {2630438656.1243875987-at-ls0130.lifesci.susx.ac.uk}
8, 25 -- In-Reply-To: {p06230901c6498abd302f-at-[140.251.48.23]}
8, 25 -- Originator-Info: login-token=Mulberry:01dHG2fdbSHOSosRbX3dWi5Cvw6NYhpNXMouYh;
8, 25 -- token_authority=postmaster-at-central.susx.ac.uk
8, 25 -- X-Mailer: Mulberry/2.0.8 (Win32)
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed
8, 25 -- Content-Disposition: inline
8, 25 -- X-Sussex: true
8, 25 -- X-Sussex-transport: remote_smtp_rew
8, 25 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk}
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n51G6SCQ016773
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Tue, 2 Jun 2009 04:08:43 -0500
Subject: [Microscopy] SEM and TEM Column Orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Late replying to this thread (it was half term here last week so I was
at home) but it bought back happy memories of my first experience of
using a TEM. More years ago than I care to remember I started as a
trainee technician in the EM lab of the school of biological science at
Leicester University and first learnt to use an AEI Corinth.
The only problem I had initially was banging my knees against the
objective aperture control!
Happy days.

Nikki Weston

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
5, 35 -- From Nicola.Weston-at-nottingham.ac.uk Tue Jun 2 04:08:42 2009
5, 35 -- Received: from ixe-mta-23.emailfiltering.com (ixe-mta-23-tx.emailfiltering.com [194.116.199.156])
5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5298fei018409
5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2009 04:08:41 -0500
5, 35 -- Received: from smtp2.nottingham.ac.uk ([128.243.44.5])
5, 35 -- by ixe-mta-23.emailfiltering.com with emfmta (version 3.7.1.44.1.r-3.2.3-libc2.3.2) vanilla id 8320690
5, 35 -- for microscopy-at-microscopy.com; Tue, 02 Jun 2009 10:08:39 +0100
5, 35 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
5, 35 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
5, 35 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 35 -- id 1MBPyX-00042X-PP
5, 35 -- for microscopy-at-microscopy.com; Tue, 02 Jun 2009 10:08:05 +0100
5, 35 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 35 -- Tue, 2 Jun 2009 10:07:07 +0100
5, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 35 -- Content-class: urn:content-classes:message
5, 35 -- MIME-Version: 1.0
5, 35 -- Content-Type: text/plain;
5, 35 -- charset="us-ascii"
5, 35 -- Subject: SEM and TEM Column Orientation
5, 35 -- Date: Tue, 2 Jun 2009 10:07:07 +0100
5, 35 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94018C4B93-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 35 -- X-MS-Has-Attach:
5, 35 -- X-MS-TNEF-Correlator:
5, 35 -- Thread-Topic: SEM and TEM Column Orientation
5, 35 -- Thread-Index: AcnjYYEyQQfWtYTkSfeDKysO3s7Npw==
5, 35 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 35 -- To: {microscopy-at-microscopy.com}
5, 35 -- X-OriginalArrivalTime: 02 Jun 2009 09:07:08.0075 (UTC) FILETIME=[817FF7B0:01C9E361]
5, 35 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
5, 35 -- X-UoN-MailScanner: Found to be clean
5, 35 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 35 -- X-Spam-Status: No
5, 35 -- Content-Transfer-Encoding: 8bit
5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5298fei018409
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 2 Jun 2009 04:27:48 -0500
Subject: [Microscopy] Outlook autocomplete

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michael,

I meant to chat to Natalie yesterday, as she normally does the inductions
[safety talk/registration]. She's quite busy at the moment, so I can do the
induction if she's unavailable. How about tomorrow [Wednesday]
morning/afternoon? Say 10.00 or 2.00.

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


-----Original Message-----
X-from: Michael Mccarthy [mailto:michael.mccarthy-at-balliol.ox.ac.uk]
Sent: 01 June 2009 15:20
To: Keith Morris

Hi all,

Sorry for the last email. I meant to send it to our Core staff via
microscopy-at-well.ox.ac.uk. Been carefully avoiding this for two years, but
finally it happened [twice now, once to the confocal list-server].


Following on from this thread, if you want to delete an email address etc..
from an auto-complete tab do the following:

Delete a name from the AutoComplete list:
Select the unwanted name using the UP ARROW or DOWN ARROW.
Press DELETE.

Turn off AutoComplete name suggesting completely in Outlook:
On the Tools menu, click Options.
On the Preferences tab, click E-mail Options, and then click Advanced E-mail
Options.
Clear the Suggest names while completing To, Cc, and Bcc fields check box.


Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/




==============================Original Headers==============================
13, 20 -- From kjmorris-at-well.ox.ac.uk Tue Jun 2 04:27:48 2009
13, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n529RlOb014572
13, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Jun 2009 04:27:47 -0500
13, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
13, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
13, 20 -- id 1MBQHb-0000sK-Ce
13, 20 -- for Microscopy-at-Microscopy.Com; Tue, 02 Jun 2009 10:27:47 +0100
13, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
13, 20 -- To: {Microscopy-at-Microscopy.Com}
13, 20 -- Subject: Outlook autocomplete
13, 20 -- Date: Tue, 2 Jun 2009 10:29:30 +0100
13, 20 -- Message-ID: {371864BA87E549CDB7CC21428B9C18D0-at-CytoWhizz}
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain;
13, 20 -- charset="US-ASCII"
13, 20 -- Content-Transfer-Encoding: 7bit
13, 20 -- X-Mailer: Microsoft Office Outlook 11
13, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
13, 20 -- Thread-Index: AcnjZKGQYKeF2iX1Tfmdonxmxqc3ig==
==============================End of - Headers==============================




From: schaffne-at-patho.unibe.ch
Date: Tue, 2 Jun 2009 07:38:24 -0500
Subject: [Microscopy] viaWWW: reembedment of thick epon sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both schaffne-at-patho.unibe.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: schaffne-at-patho.unibe.ch
Name: Thomas Schaffner

Organization: Dept. of Pathology University of Bern Switzerland

Title-Subject: [Filtered] reembedment of thick epon sections

Question: Hallo Se Pyo

we have used a diamond pen to mark/scratch the interesting area on
the bottom of the slide carrying the thick section. Then we removed
the coverslip with xylene and carried the thick section through the
terminal solvents and embedding mixtures of our preferred resin to
finally cover the area of interest with a BEEM or other capsule
turned upside down (you have to cut the lid off). The scratch on the
bottom of the slide serves to center the capsule since the dye might
have largely come off the section. You then cure the new block on the
slide. To remove the block from the glass after curing place the
slide on dry ice for a minute then immerse it in luke warm water for
a minute. Repeat this cold/warm sequence patiently until the block
falls off.
Greetings from Switzerland

Thomas Schaffner
Professor of Pathology
University of Bern

Login Host: 130.92.9.57
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Jun 2 07:38:24 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n52CcN8p003235
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2009 07:38:23 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c64acdaa1142-at-[206.69.208.22]}
8, 11 -- Date: Tue, 2 Jun 2009 07:38:21 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: schaffne-at-patho.unibe.ch (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: reembedment of thick epon sections
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rojasvidovic-at-unina.it
Date: Tue, 2 Jun 2009 07:39:03 -0500
Subject: [Microscopy] viaWWW: freeze cutting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rojasvidovic-at-unina.it as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rojasvidovic-at-unina.it
Name: Juan Carlos Rojas

Organization: University of Glasgow

Title-Subject: [Filtered] freeze cutting

Question: Dear all,

Perhaps you can help me (or direct to an appropriate source) with
this query ...

I'm interested in conduct Environmenatl Scanning Electron Microscopy
(ESEM) analysis to study microstructures of fine grained soils.

Specimen preparation for ESEM analysis includes the creation of a
flat and smooth observation surface. In order to minimise
microstructural changes I will use the freeze cut method.

In the freeze cut method, a soil specimen is cut in a circular plate
shape with a diameter of 18 mm and a heigth of 3 mm. The specimen is
dipped into liquid nitrogen. The specimen is then cut into two parts
with a cooled sharp knife to generate, as mentioned above, a smooth
flat surface.

My question is: What kind of knife should I use?. Searching in
internet I found some high quality single-edge razor blades but were
not designed to cut ice.

Any clarification (or direction towards appropriate sources) will be
very helpful. Thanks for your time.

Best Regards,

Juan Carlos Rojas

Login Host: 130.209.6.42
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Tue Jun 2 07:39:03 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n52Cd2Ax004175
14, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2009 07:39:03 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240802c64acdc41735-at-[206.69.208.22]}
14, 11 -- Date: Tue, 2 Jun 2009 07:39:01 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: rojasvidovic-at-unina.it (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: freeze cutting
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: smithj-at-winthrop.edu
Date: Tue, 2 Jun 2009 08:40:02 -0500
Subject: [Microscopy] 2-color photoconversion?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all (with apologies for duplicate posting to those of you who
belong to both the confocal and microscopy listservs)
We're about to start a series of correlative experiments relating
confocal fluorescence to info from LM sections. We're using
AlexaFluor488 and AlexaFluor568, and using wholemounts of small (2mm by
200µm) worms. The current plan is to photoconvert the dye using DAB,
dehydrate, and embed in either paraffin or "Epon"/Araldite for sectioning.
I've found plenty of protocols for DAB photoconversion, and we're
starting those experiments this week. Does anyone know of a protocol
for the photoconversion of another readily-oxidized dye to give a
different color (other than the brown of DAB), so we can see both dyes
at once in our sections?
TIA,
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)


==============================Original Headers==============================
4, 21 -- From smithj-at-winthrop.edu Tue Jun 2 08:40:01 2009
4, 21 -- Received: from messenger.winthrop.edu (messenger.winthrop.edu [199.79.254.177])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n52De1pN001335
4, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2009 08:40:01 -0500
4, 21 -- Received: from berlin.win.winthrop.edu (berlin.win.winthrop.edu [10.2.0.22])
4, 21 -- by messenger.winthrop.edu (MOS 3.8.6-GA)
4, 21 -- with ESMTP id GUJ06312;
4, 21 -- Tue, 2 Jun 2009 09:39:52 -0400 (EDT)
4, 21 -- Received: from Julian-Smiths-Computer.local ([10.3.84.33]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959);
4, 21 -- Tue, 2 Jun 2009 09:39:52 -0400
4, 21 -- Message-ID: {4A252BA8.9000906-at-winthrop.edu}
4, 21 -- Date: Tue, 02 Jun 2009 09:39:52 -0400
4, 21 -- From: Julian Smith III {smithj-at-winthrop.edu}
4, 21 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
4, 21 -- MIME-Version: 1.0
4, 21 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} ,
4, 21 -- microscopy-at-microscopy.com
4, 21 -- Subject: 2-color photoconversion?
4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 21 -- Content-Transfer-Encoding: 8bit
4, 21 -- X-OriginalArrivalTime: 02 Jun 2009 13:39:52.0159 (UTC) FILETIME=[9B4106F0:01C9E387]
==============================End of - Headers==============================




From: RonMervis-at-neurostructural.org
Date: Wed, 3 Jun 2009 20:28:46 -0500
Subject: [Microscopy] viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both RonMervis-at-neurostructural.org as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: RonMervis-at-neurostructural.org
Name: Ron Mervis

Organization: Neurostructural Research Labs

Title-Subject: [Filtered] double images in microscope

Question: good morning...
i have a new student to whom i am teaching microscopy...we have Zeiss
brightfield research microscopes...
the problem i seem to have with him (and which i haven't had with
others) is that he consistently sees double images which overlap and
which he can't seem to get to converge...
the oculars are adjustable and he has moved them around to try to
remedy the problem -- but it hasn't helped...he occasionally wears
glasses (he's a bit farsighted)...but otherwise his vision is
perfectly normal...

any suggestions???

thanks...
ron mervis

Login Host: 64.12.117.15
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Jun 3 20:28:46 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n541Sjuw031726
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2009 20:28:45 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c64cd3b17d4d-at-[206.69.208.22]}
8, 11 -- Date: Wed, 3 Jun 2009 20:28:44 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: RonMervis-at-neurostructural.org (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: double images in microscope
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rana.kasim-at-ist.utl.pt
Date: Wed, 3 Jun 2009 20:29:27 -0500
Subject: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rana.kasim-at-ist.utl.pt as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rana.kasim-at-ist.utl.pt
Name: Rana Kasim

Organization: Ph.D student Instituto Superior
TÈcnicoMaterials Engineering
Department-university of lisbon

Title-Subject: [Filtered] Monte carlo simulation with EDS analysis

Question: Hi All,
Could any person help me to how i can get the
Monte carlo simulation with Energy Dispersive
Spectroscopy(EDS)and the software that can
calculate the light volume when transmitt inside
the tissue and its pentration depth.

Regards
Rana

Login Host: 193.136.139.253
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Wed Jun 3 20:29:27 2009
8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n541TQd4032314
8, 13 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2009 20:29:27 -0500
8, 13 -- Mime-Version: 1.0
8, 13 -- Message-Id: {p06240802c64cd3d8866b-at-[206.69.208.22]}
8, 13 -- Date: Wed, 3 Jun 2009 20:29:26 -0500
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: rana.kasim-at-ist.utl.pt (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: Monte carlo simulation with EDS analysis
8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 13 -- Content-Transfer-Encoding: 8bit
8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n541TQd4032314
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Thu, 4 Jun 2009 02:54:43 -0500
Subject: [Microscopy] Re: viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Just a questions and two possible answers :

Does this probleme occur on equal which microscope, or always,
independently of the microsocpe ?

In case it's microscope independent, maybe it's an accomodation
diffucly. Tell him he must try to look /"trought"/ the microscope, and
not /"in"/ the microscope. The eyes must be relaxed, as if he would be
looking at infinity, and not trying to accomodate at short distance.
Some people have difficulties to do this. He can try, siting at the
microscope, to look first out trough the window on something far away,
an then to go to the oculars. Of coarse the interpupillar distance must
be right. But for that, he can ask to an optician to measure it, and set
this measured value on the microscope head, as a starting point.

In case it is only on one specific microscope, it could be that the
binocular head has a collimation error, that he cannot compensate. Other
users won't have the difficulty as they are familiar with the
microscope, or are more trained to compensate. If it is the case, you
would have interesst to correct it. A collimation error can be a big
source of weariness, eyestrain and headeach. But such errors aren't
frequent on OM, more on consumer type binoculars.


Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



RonMervis-at-neurostructural.org a écrit :
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both RonMervis-at-neurostructural.org as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: RonMervis-at-neurostructural.org
} Name: Ron Mervis
}
} Organization: Neurostructural Research Labs
}
} Title-Subject: [Filtered] double images in microscope
}
} Question: good morning...
} i have a new student to whom i am teaching microscopy...we have Zeiss
} brightfield research microscopes...
} the problem i seem to have with him (and which i haven't had with
} others) is that he consistently sees double images which overlap and
} which he can't seem to get to converge...
} the oculars are adjustable and he has moved them around to try to
} remedy the problem -- but it hasn't helped...he occasionally wears
} glasses (he's a bit farsighted)...but otherwise his vision is
} perfectly normal...
}
} any suggestions???
}
} thanks...
} ron mervis
}
} Login Host: 64.12.117.15
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Wed Jun 3 20:28:46 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n541Sjuw031726
} 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2009 20:28:45 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c64cd3b17d4d-at-[206.69.208.22]}
} 8, 11 -- Date: Wed, 3 Jun 2009 20:28:44 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: RonMervis-at-neurostructural.org (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: double images in microscope
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
12, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jun 4 02:54:43 2009
12, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154])
12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n547sge4015897
12, 32 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 02:54:42 -0500
12, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
12, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n547seKE016886
12, 32 -- ; Thu, 4 Jun 2009 09:54:40 +0200 (CEST)
12, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
12, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
12, 32 -- (No client certificate requested)
12, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 967CF100021F;
12, 32 -- Thu, 4 Jun 2009 09:48:12 +0200 (CEST)
12, 32 -- Message-ID: {4A277DAE.3070404-at-ipcms.u-strasbg.fr}
12, 32 -- Date: Thu, 04 Jun 2009 09:54:22 +0200
12, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
12, 32 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
12, 32 -- MIME-Version: 1.0
12, 32 -- To: RonMervis-at-neurostructural.org, Microscopy-at-microscopy.com
12, 32 -- Subject: Re: [Microscopy] viaWWW: double images in microscope
12, 32 -- References: {200906040137.n541bSU7016129-at-ns.microscopy.com}
12, 32 -- In-Reply-To: {200906040137.n541bSU7016129-at-ns.microscopy.com}
12, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
12, 32 -- Content-Transfer-Encoding: 8bit
12, 32 -- X-IPCMS-MailScanner: Found to be clean
12, 32 -- X-IPCMS-MailScanner-SpamScore: s
12, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
12, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 04 Jun 2009 09:54:40 +0200 (CEST)
12, 32 -- X-Virus-Scanned: ClamAV 0.94.2/9419/Thu Jun 4 04:47:46 2009 on mr4.u-strasbg.fr
12, 32 -- X-Virus-Status: Clean
12, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
12, 32 -- autolearn=disabled version=3.2.5
12, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Thu, 4 Jun 2009 05:54:35 -0500
Subject: [Microscopy] Re: viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds like the interpupillary distance might not be set correctly for this user.
The eyepieces are either too far apart or too close together. Try adjusting that
until the images converge (without the glasses).

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329


On Wed Jun 3 21:29 , RonMervis-at-neurostructural.org sent:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America




==============================Original Headers==============================
8, 32 -- From SRS0=FDoHuB=B7=metallography.com=jeff-at-eigbox.net Thu Jun 4 05:54:34 2009
8, 32 -- Received: from bosmailout17.eigbox.net (bosmailout17.eigbox.net [66.96.184.17])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n54AsYGP007206
8, 32 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 05:54:34 -0500
8, 32 -- Received: from bosmailscan20.eigbox.net ([10.20.15.20])
8, 32 -- by bosmailout17.eigbox.net with esmtp (Exim)
8, 32 -- id 1MCAag-0002VA-6t
8, 32 -- for microscopy-at-microscopy.com; Thu, 04 Jun 2009 06:54:34 -0400
8, 32 -- Received: from bosimpout01.eigbox.net ([10.20.55.1])
8, 32 -- by bosmailscan20.eigbox.net with esmtp (Exim)
8, 32 -- id 1MCAag-0002DB-0H
8, 32 -- for microscopy-at-microscopy.com; Thu, 04 Jun 2009 06:54:34 -0400
8, 32 -- Received: from boswebmail22.eigbox.net ([10.20.16.22])
8, 32 -- by bosimpout01.eigbox.net with NO UCE
8, 32 -- id zilP1b0040UZg7e0000000; Thu, 04 Jun 2009 02:45:23 -0400
8, 32 -- X-EN-OrigOutIP: 10.20.16.22
8, 32 -- X-EN-IMPSID: zilP1b0040UZg7e0000000
8, 32 -- Received: from [72.74.219.78] by boswebmail22.eigbox.net via HTTP; Thu, 04 Jun 2009 06:54:21 -0400
8, 32 -- Content-Disposition: inline
8, 32 -- Content-Type: text/plain; charset="iso-8859-1"
8, 32 -- MIME-Version: 1.0
8, 32 -- From: {jeff-at-metallography.com}
8, 32 -- To: Microscopy-at-microscopy.com
8, 32 -- Subject: Re: [Microscopy] viaWWW: double images in microscope
8, 32 -- Reply-To: jeff-at-metallography.com
8, 32 -- X-Origin: 72.74.219.78
8, 32 -- Date: Thu, 04 Jun 2009 06:54:13 -0400
8, 32 -- Message-Id: {44660.1244112853-at-metallography.com}
8, 32 -- X-Mailer: AtMail 4.61 - 72.74.219.78 - jeff-at-metallography.com
8, 32 -- Sender: {jeff-at-metallography.com}
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n54AsYGP007206
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 4 Jun 2009 08:23:10 -0500
Subject: [Microscopy] RE: viaWWW: Monte carlo simulation with EDS analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can try "Casino", which is, I believe, freeware:
http://www.gel.usherbrooke.ca/casino/What.html

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: rana.kasim-at-ist.utl.pt [mailto:rana.kasim-at-ist.utl.pt]
} Sent: Wednesday, June 03, 2009 8:30 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both rana.kasim-at-ist.utl.pt as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: rana.kasim-at-ist.utl.pt
} Name: Rana Kasim
}
} Organization: Ph.D student Instituto Superior
} TÈcnicoMaterials Engineering Department-university of lisbon
}
} Title-Subject: [Filtered] Monte carlo simulation with EDS analysis
}
} Question: Hi All,
} Could any person help me to how i can get the Monte carlo
} simulation with Energy Dispersive Spectroscopy(EDS)and the
} software that can calculate the light volume when transmitt
} inside the tissue and its pentration depth.
}
} Regards
} Rana
}
} Login Host: 193.136.139.253
} --------------------------------------------------------------
} -------------
}
}
} ==============================Original
} Headers==============================
} 8, 13 -- From zaluzec-at-microscopy.com Wed Jun 3 20:29:27 2009
} 8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n541TQd4032314
} 8, 13 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun
} 2009 20:29:27 -0500
} 8, 13 -- Mime-Version: 1.0
} 8, 13 -- Message-Id: {p06240802c64cd3d8866b-at-[206.69.208.22]}
} 8, 13 -- Date: Wed, 3 Jun 2009 20:29:26 -0500 8, 13 -- To:
} microscopy-at-microscopy.com 8, 13 -- From:
} rana.kasim-at-ist.utl.pt (by way of MicroscopyListserver) 8, 13
} -- Subject: viaWWW: Monte carlo simulation with EDS analysis
} 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ;
} format="flowed"
} 8, 13 -- Content-Transfer-Encoding: 8bit 8, 13 --
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n541TQd4032314
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
6, 25 -- From DusevichV-at-umkc.edu Thu Jun 4 08:23:10 2009
6, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n54DN8bU026519
6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 08:23:09 -0500
6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Thu, 4 Jun 2009 08:23:06 -0500
6, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="iso-8859-1"
6, 25 -- Subject: RE: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis
6, 25 -- Date: Thu, 4 Jun 2009 08:23:05 -0500
6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB867-at-KC-MSX1.kc.umkc.edu}
6, 25 -- In-Reply-To: {200906040129.n541Tusk001241-at-ns.microscopy.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis
6, 25 -- Thread-Index: Acnks/iFFG6w/RwCQS2xrKe6YDEkxAAY3UTw
6, 25 -- References: {200906040129.n541Tusk001241-at-ns.microscopy.com}
6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
6, 25 -- To: {rana.kasim-at-ist.utl.pt} , {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 04 Jun 2009 13:23:06.0297 (UTC) FILETIME=[988A2A90:01C9E517]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n54DN8bU026519
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Thu, 4 Jun 2009 11:34:13 -0500
Subject: [Microscopy] Frigocut 2800 manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would any of you have (or know where to obtain) a manual (paper or pdf)
for a Leica Jung Frigocut 2800E cryostat?

Thanks,

Andy Bowling


==============================Original Headers==============================
4, 31 -- From AJBowling-at-dow.com Thu Jun 4 11:34:13 2009
4, 31 -- Received: from mail168.messagelabs.com (mail168.messagelabs.com [216.82.253.195])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n54GYCMF016223
4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 11:34:13 -0500
4, 31 -- X-VirusChecked: Checked
4, 31 -- X-Env-Sender: AJBowling-at-dow.com
4, 31 -- X-Msg-Ref: server-7.tower-168.messagelabs.com!1244133237!14443182!10
4, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,-
4, 31 -- X-Originating-IP: [204.136.184.21]
4, 31 -- Received: (qmail 28435 invoked from network); 4 Jun 2009 16:34:09 -0000
4, 31 -- Received: from mail3.dow.com (HELO USFRPMDOWS001.dow.com) (204.136.184.21)
4, 31 -- by server-7.tower-168.messagelabs.com with RC4-SHA encrypted SMTP; 4 Jun 2009 16:34:09 -0000
4, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USFRPMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.3959);
4, 31 -- Thu, 4 Jun 2009 11:34:07 -0500
4, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
4, 31 -- Content-class: urn:content-classes:message
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- charset="us-ascii"
4, 31 -- Subject: Frigocut 2800 manual
4, 31 -- Date: Thu, 4 Jun 2009 12:34:06 -0400
4, 31 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA10103C46A-at-USMDLMDOWX032.dow.com}
4, 31 -- X-MS-Has-Attach:
4, 31 -- X-MS-TNEF-Correlator:
4, 31 -- Thread-Topic: Frigocut 2800 manual
4, 31 -- Thread-Index: AcnlMkcdjwoPyuVVRJOeONyYm+5dHA==
4, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
4, 31 -- To: {microscopy-at-microscopy.com}
4, 31 -- X-OriginalArrivalTime: 04 Jun 2009 16:34:07.0801 (UTC) FILETIME=[4820E290:01C9E532]
4, 31 -- Content-Transfer-Encoding: 8bit
4, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n54GYCMF016223
==============================End of - Headers==============================




From: richard.ross-at-allisontransmission.com
Date: Thu, 4 Jun 2009 18:24:51 -0500
Subject: [Microscopy] viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both richard.ross-at-allisontransmission.com as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: richard.ross-at-allisontransmission.com
Name: Richard Ross

Organization: Allison Transmission Inc.

Title-Subject: [Microscopy] viaWWW: double images in microscope

Question: Could this person be one of the 10-15% of the population
that does not have binocular vision? I am one of these, and my
less-dominant eye is ignored for my central vision, so no microscope
issues (other than no stereo 3-d capability!). Perhaps this
individual's visual cortex is develeoped somewhere between binocular
and monocular vision and has trouble fusing the view presented in a
microscope. Just some thoughts to consider.



Login Host: 12.53.132.4
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 4 18:24:49 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n54NOl8r014610
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 18:24:48 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c64e082dcbc0-at-[206.69.208.22]}
8, 11 -- Date: Thu, 4 Jun 2009 18:24:46 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: richard.ross-at-allisontransmission.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: double images in microscope
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 5 Jun 2009 03:18:48 -0500
Subject: [Microscopy] TEM Mohs Scale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is it possible, for the commonly used resins in TEM embedding (like Epon, Durcupan / Araldite, ERL, LR White or Gold, Lowi's, etc), to give a figure for their relative hardness on the Mohs hardness scale?
relative to Copper and Gold, e.g. ?
Does anybody know a source for this type of information?
best regards,
Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
6, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jun 5 03:18:48 2009
6, 23 -- Received: from rrzmta4.rz.uni-regensburg.de (rrzmta4.rz.uni-regensburg.de [194.94.155.55])
6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n558IhX1009805
6, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 03:18:48 -0500
6, 23 -- Received: from rrzmta4.rz.uni-regensburg.de (localhost [127.0.0.1])
6, 23 -- by localhost (Postfix) with SMTP id A4C54775
6, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:18:21 +0200 (CEST)
6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
6, 23 -- by rrzmta4.rz.uni-regensburg.de (Postfix) with ESMTP id 8853B752
6, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:18:21 +0200 (CEST)
6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
6, 23 -- with Novell_GroupWise; Fri, 05 Jun 2009 10:18:42 +0200
6, 23 -- Message-Id: {4A28F0FF0200005400015FE8-at-gwsmtp1.uni-regensburg.de}
6, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
6, 23 -- Date: Fri, 05 Jun 2009 10:18:39 +0200
6, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
6, 23 -- To: {Microscopy-at-microscopy.com}
6, 23 -- Subject: TEM Mohs Scale
6, 23 -- Mime-Version: 1.0
6, 23 -- Content-Type: text/plain; charset=US-ASCII
6, 23 -- Content-Disposition: inline
6, 23 -- Content-Transfer-Encoding: 8bit
6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n558IhX1009805
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Fri, 5 Jun 2009 04:39:19 -0500
Subject: [Microscopy] viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have 'double vision', although this has been minimised with corrective
surgery a few years ago [my 'bad' right eye had drifted away from that of my
childhood and my brain was starting to process the visual info again from it
- so they put the eye pointing inwards again to switch it 'off', as the
brain was used to ignoring it there].

All this was from a lazy eye [squint] at childhood though and a clear visual
defect. In fact I keep the microscope binoculars open too wide so I only
look down with the left [good] eye* - I shift the good eye from right to
left binocular during cleaning for the users. However my lazy eye really
counts as a major visual problem and apparently your user hasn't got
anything like that [my right eyes perfect just the brain doesn't process the
info as it didn't like the double vision either when growing during my
youth]. Visual/cortex problems can produce odd effects - there's one where
people think they see little grey people...

I expect straining to peer down the microscope is knocking the eye out of 3D
alignment [you can make the eyes squint inwards if you try - personally I
don't], but perhaps someone from optimetrics can advise. Try the monocular
approach as well.

Keith

*I don't suppose many remember the monocular microscopes of yore, except
perhaps from school, but there's no stereoscopic info on a compound
microscope so monocular is fine. Not that I'd see 3D information anyway - in
fact we make good photographers, probably because we have an eye for the 2D
scene.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: richard.ross-at-allisontransmission.com
[mailto:richard.ross-at-allisontransmission.com]
Sent: 05 June 2009 00:31
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both richard.ross-at-allisontransmission.com as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: richard.ross-at-allisontransmission.com
Name: Richard Ross

Organization: Allison Transmission Inc.

Title-Subject: [Microscopy] viaWWW: double images in microscope

Question: Could this person be one of the 10-15% of the population
that does not have binocular vision? I am one of these, and my
less-dominant eye is ignored for my central vision, so no microscope
issues (other than no stereo 3-d capability!). Perhaps this
individual's visual cortex is develeoped somewhere between binocular
and monocular vision and has trouble fusing the view presented in a
microscope. Just some thoughts to consider.



Login Host: 12.53.132.4
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 4 18:24:49 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n54NOl8r014610
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 18:24:48
-0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c64e082dcbc0-at-[206.69.208.22]}
8, 11 -- Date: Thu, 4 Jun 2009 18:24:46 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: richard.ross-at-allisontransmission.com (by way of
MicroscopyListserver)
8, 11 -- Subject: viaWWW: double images in microscope
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 22 -- From kjmorris-at-well.ox.ac.uk Fri Jun 5 04:39:19 2009
20, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n559dJe8027496
20, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 5 Jun 2009 04:39:19 -0500
20, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
20, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
20, 22 -- id 1MCVtO-00024X-GL
20, 22 -- for Microscopy-at-Microscopy.Com; Fri, 05 Jun 2009 10:39:18 +0100
20, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
20, 22 -- To: {Microscopy-at-Microscopy.Com}
20, 22 -- References: {200906042331.n54NVMor024949-at-ns.microscopy.com}
20, 22 -- Subject: RE: [Microscopy] viaWWW: double images in microscope
20, 22 -- Date: Fri, 5 Jun 2009 10:39:18 +0100
20, 22 -- Message-ID: {33EBE5989E74400F8D4CD698B44D3BA3-at-CytoWhizz}
20, 22 -- MIME-Version: 1.0
20, 22 -- Content-Type: text/plain;
20, 22 -- charset="us-ascii"
20, 22 -- Content-Transfer-Encoding: 7bit
20, 22 -- X-Mailer: Microsoft Office Outlook 11
20, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 22 -- Thread-Index: AcnlbJJFtu1n4AzzQCe0EwvkdkdpPAAUdqgg
20, 22 -- In-Reply-To: {200906042331.n54NVMor024949-at-ns.microscopy.com}
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Fri, 5 Jun 2009 08:29:56 -0500
Subject: [Microscopy] Re: Reembedment of a semithin section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Se Pyo Hong,

it seems to me that there weren't many replies to your question...as far as from replies via the MSA Listserver.

I would like to add a reference for an article, published most recently on this topic:

Cf.
SAWAGUCHI A, AOYAMA F, IDE S & SUGANUMA T:
Capsule-Supporting ring: a new device for resin embedding of glass-mounted specimens.
J. Micr. 234 pt 2(no. 849),pp. 113-117, 2009

Special reference to:
- Re-embedding of epoxy-resin semithin sections for morphological observation,
- Re-embedding of Lowicryl K4M semithin sections for immunocytochemical observation
- in situ flat embedding of cultured cells on glass cover slip

You'll find a very sophisticated technique, sufficiently illustrated, to reembed distinct and small areas from a thick (semithin) resin section for a subsequent (T)EM-observation.

If you can't retrieve the paper from the Journal I could help you with a pdf of the article.

Best wishes and good luck,
have a nice weekend,


Wolfgang MUSS
EM-Lab/Pathology
SALK-LKH (Gen.Hosp.) - PMU (Priv. Paracelsus Med. Univ.) SALZBURG
AUSTRIA-Europe






} -----Ursprüngliche Nachricht-----
} Von: hong_s-at-palmer.edu [mailto:hong_s-at-palmer.edu]
} Gesendet: Mittwoch, 27. Mai 2009 21:33
} An: Muß Wolfgang
} Betreff: [Microscopy] Reembedment of a semithin section
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both hong_s-at-palmer.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
} Email: hong_s-at-palmer.edu
} Name: Se Pyo Hong
} Organization: Graduate Studies at Palmer College of Chiropractic
} Title-Subject: reembedment of a semithin section
} Question:
}
}
} Hi everyone;
} I have 2-micron thick flat specimens (cross sectioned rat
} spinal cord) embedded in epon with toluidine stain on a glass
} slide. I want to have ultrathin sections of part of the
} specimen (Raxed lamina 1 - 3 area).
} Please give me suggestions or a procedure. Thank you..
}
}
} Login Host: 198.102.161.2
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Wed May 27 14:28:30 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJSTj8019343
} 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:28:29 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240801c64344cbf431-at-[206.69.208.22]}
} 7, 11 -- Date: Wed, 27 May 2009 14:28:27 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: hong_s-at-palmer.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: reembedment of a semithin section
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of Headers==============================
}


==============================Original Headers==============================
17, 36 -- From W.Muss-at-salk.at Fri Jun 5 08:29:55 2009
17, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
17, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55DTqJf015595
17, 36 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 08:29:54 -0500
17, 36 -- Received: from localhost (localhost [127.0.0.1])
17, 36 -- by hermes.salk.at (Postfix) with ESMTP id 55D69C3856;
17, 36 -- Fri, 5 Jun 2009 15:29:47 +0200 (CEST)
17, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
17, 36 -- Received: from hermes.salk.at ([127.0.0.1])
17, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
17, 36 -- with ESMTP id 5-e4JbdQRJvI; Fri, 5 Jun 2009 15:29:46 +0200 (CEST)
17, 36 -- Received: from n2rz123.lksdom21.lks.local (n2rz123.lksdom21.lks.local [192.168.101.123])
17, 36 -- by hermes.salk.at (Postfix) with ESMTP id DD3A6C3875;
17, 36 -- Fri, 5 Jun 2009 15:29:46 +0200 (CEST)
17, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
17, 36 -- Fri, 5 Jun 2009 15:29:47 +0200
17, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 36 -- Content-class: urn:content-classes:message
17, 36 -- MIME-Version: 1.0
17, 36 -- Content-Type: text/plain;
17, 36 -- charset="iso-8859-1"
17, 36 -- Subject: [Microscopy] Re: Reembedment of a semithin section
17, 36 -- Date: Fri, 5 Jun 2009 15:29:46 +0200
17, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062938715-at-N1RZ116.lksdom21.lks.local}
17, 36 -- In-Reply-To: {200905271933.n4RJXSkk030084-at-ns.microscopy.com}
17, 36 -- X-MS-Has-Attach:
17, 36 -- X-MS-TNEF-Correlator:
17, 36 -- Thread-Topic: [Microscopy] Re: Reembedment of a semithin section
17, 36 -- Thread-Index: AcnfAgfphaNX5HVORKOAASO2yByKxAG3eyOQ
17, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
17, 36 -- To: {hong_s-at-palmer.edu}
17, 36 -- Cc: {microscopy-at-microscopy.com}
17, 36 -- X-OriginalArrivalTime: 05 Jun 2009 13:29:47.0087 (UTC) FILETIME=[B1D79DF0:01C9E5E1]
17, 36 -- X-Scanned-By: SALK-Content-Filter
17, 36 -- Content-Transfer-Encoding: 8bit
17, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55DTqJf015595
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Fri, 5 Jun 2009 09:30:47 -0500
Subject: [Microscopy] viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ron,
I always had double vision when using binocular eyepieces on binoculars as a child and through college, and never needed or wore glasses. For my first job in EM after college, I still had double-vision when using binocular microscopes. After 3-5 months of sectioning on the ultramicrotome and favoring one eye, I was looking at a semithin section on a basic binocular compound microscope one day, and the two images slowly started moving together until they overlapped. I didn't move at all during this 1-2 second experience. (It probably only seemed that long, though.) I have been able to use binocular microscopes correctly ever since.

My theory is that my brain just needed to adjust to this uncommon phenomenon of binocular eyepieces, and merely took longer than the average person.

As a follow-up: When I got eyeglasses a few years afterwards, I had a terrible time adjusting to every new prescription for several years. Now the adjustment is very brief.

Good luck to your student. Advise them to stick with it, and it may correct itself.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

"We are all individuals!" -- Monty Python, The Life of Brian. (The quote seems appropriate here.)



-----Original Message-----
X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk]
Sent: Friday, June 05, 2009 5:46 AM
To: Sobocinski, Gregg

I have 'double vision', although this has been minimised with corrective
surgery a few years ago [my 'bad' right eye had drifted away from that of my
childhood and my brain was starting to process the visual info again from it
- so they put the eye pointing inwards again to switch it 'off', as the
brain was used to ignoring it there].

All this was from a lazy eye [squint] at childhood though and a clear visual
defect. In fact I keep the microscope binoculars open too wide so I only
look down with the left [good] eye* - I shift the good eye from right to
left binocular during cleaning for the users. However my lazy eye really
counts as a major visual problem and apparently your user hasn't got
anything like that [my right eyes perfect just the brain doesn't process the
info as it didn't like the double vision either when growing during my
youth]. Visual/cortex problems can produce odd effects - there's one where
people think they see little grey people...

I expect straining to peer down the microscope is knocking the eye out of 3D
alignment [you can make the eyes squint inwards if you try - personally I
don't], but perhaps someone from optimetrics can advise. Try the monocular
approach as well.

Keith

*I don't suppose many remember the monocular microscopes of yore, except
perhaps from school, but there's no stereoscopic info on a compound
microscope so monocular is fine. Not that I'd see 3D information anyway - in
fact we make good photographers, probably because we have an eye for the 2D
scene.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: richard.ross-at-allisontransmission.com
[mailto:richard.ross-at-allisontransmission.com]
Sent: 05 June 2009 00:31
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both richard.ross-at-allisontransmission.com as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: richard.ross-at-allisontransmission.com
Name: Richard Ross

Organization: Allison Transmission Inc.

Title-Subject: [Microscopy] viaWWW: double images in microscope

Question: Could this person be one of the 10-15% of the population
that does not have binocular vision? I am one of these, and my
less-dominant eye is ignored for my central vision, so no microscope
issues (other than no stereo 3-d capability!). Perhaps this
individual's visual cortex is develeoped somewhere between binocular
and monocular vision and has trouble fusing the view presented in a
microscope. Just some thoughts to consider.



Login Host: 12.53.132.4
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 4 18:24:49 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n54NOl8r014610
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 18:24:48
-0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c64e082dcbc0-at-[206.69.208.22]}
8, 11 -- Date: Thu, 4 Jun 2009 18:24:46 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: richard.ross-at-allisontransmission.com (by way of
MicroscopyListserver)
8, 11 -- Subject: viaWWW: double images in microscope
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 22 -- From kjmorris-at-well.ox.ac.uk Fri Jun 5 04:39:19 2009
20, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n559dJe8027496
20, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 5 Jun 2009 04:39:19 -0500
20, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
20, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
20, 22 -- id 1MCVtO-00024X-GL
20, 22 -- for Microscopy-at-Microscopy.Com; Fri, 05 Jun 2009 10:39:18 +0100
20, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
20, 22 -- To: {Microscopy-at-Microscopy.Com}
20, 22 -- References: {200906042331.n54NVMor024949-at-ns.microscopy.com}
20, 22 -- Subject: RE: [Microscopy] viaWWW: double images in microscope
20, 22 -- Date: Fri, 5 Jun 2009 10:39:18 +0100
20, 22 -- Message-ID: {33EBE5989E74400F8D4CD698B44D3BA3-at-CytoWhizz}
20, 22 -- MIME-Version: 1.0
20, 22 -- Content-Type: text/plain;
20, 22 -- charset="us-ascii"
20, 22 -- Content-Transfer-Encoding: 7bit
20, 22 -- X-Mailer: Microsoft Office Outlook 11
20, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 22 -- Thread-Index: AcnlbJJFtu1n4AzzQCe0EwvkdkdpPAAUdqgg
20, 22 -- In-Reply-To: {200906042331.n54NVMor024949-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
35, 26 -- From greggps-at-umich.edu Fri Jun 5 09:30:46 2009
35, 26 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201])
35, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55EUkfJ031912
35, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 09:30:46 -0500
35, 26 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.235]) by
35, 26 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Fri, 5 Jun
35, 26 -- 2009 10:30:46 -0400
35, 26 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
35, 26 -- To: Microscopy MSA {Microscopy-at-microscopy.com} ,
35, 26 -- "RonMervis-at-neurostructural.org" {RonMervis-at-neurostructural.org}
35, 26 -- Date: Fri, 5 Jun 2009 10:30:45 -0400
35, 26 -- Subject: RE: [Microscopy] double images in microscopes-My own happy ending
35, 26 -- Thread-Topic: [Microscopy] double images in microscopes-My own happy ending
35, 26 -- Thread-Index: Acnlwmui20ZGUzIXRleF4jqQNmgjGwAJXTaQ
35, 26 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA393406143A68FE-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
35, 26 -- References: {200906050945.n559jrep006946-at-ns.microscopy.com}
35, 26 -- In-Reply-To: {200906050945.n559jrep006946-at-ns.microscopy.com}
35, 26 -- Accept-Language: en-US
35, 26 -- Content-Language: en-US
35, 26 -- X-MS-Has-Attach:
35, 26 -- X-MS-TNEF-Correlator:
35, 26 -- acceptlanguage: en-US
35, 26 -- Content-Type: text/plain; charset="us-ascii"
35, 26 -- MIME-Version: 1.0
35, 26 -- Content-Transfer-Encoding: 8bit
35, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55EUkfJ031912
==============================End of - Headers==============================




From: gul417-at-mail.usask.ca
Date: Fri, 5 Jun 2009 10:42:41 -0500
Subject: [Microscopy] SEM Philips505--Polaroid films and upgrade to digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I guess this topic has been discussed in this forum (I found that the
searching function in ListServer achieves was not so straightforward,
spoiled by Google), but I also think that there should be some newer
system(s)/technique available.

We have a 25yr-old Philips SEM 505 and are interested in upgrading it
to digital photography capability. I am looking advice for the system
should be considered. And estimated cost including computer and
software (preferably cheaper)?

In addition, does anyone still keep some Polaroid 665 films that we can
buy before ours run out?

Thank you in advance!

Guosheng Liu
Dept of Biology,
Univ of Saskatchewan
Canada


==============================Original Headers==============================
7, 20 -- From gul417-at-mail.usask.ca Fri Jun 5 10:42:37 2009
7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55FgaHK016599
7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:42:37 -0500
7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- id {0KKR00601VHZX6-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
7, 20 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2009 09:42:36 -0600 (CST)
7, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31])
7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- with SMTP id {0KKR00IV7VN09I-at-usask.ca} for Microscopy-at-microscopy.com; Fri,
7, 20 -- 05 Jun 2009 09:42:36 -0600 (CST)
7, 20 -- Date: Fri, 05 Jun 2009 09:42:35 -0600 (CST)
7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
7, 20 -- Subject: SEM Philips505--Polaroid films and upgrade to digital
7, 20 -- To: Microscopy-at-microscopy.com
7, 20 -- Message-id: {6016978.1244216555923.JavaMail.gul417-at-mail.usask.ca}
7, 20 -- MIME-version: 1.0
7, 20 -- Content-type: text/plain; charset=UTF-8
7, 20 -- Content-transfer-encoding: 7BIT
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Fri, 5 Jun 2009 11:03:35 -0500
Subject: [Microscopy] Re: SEM Philips505--Polaroid films and upgrade to digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Guosheng,
if you are able to do some soldering in the scan generator of the 505
there is a not too costly possibility to do a slow scan frame grabbing
up to two channels with an USB A/D converter, using a programm a friend
of mine wrote for his 505. In this case please reply to me offline.
Then there is - and this I use for my work - to use anexternal digital
scan generator and an external framegrabber. I use DISS5 from
www.pointelectronic.de/english/products/diss5_english.htm
With software this solution starts at ca. 8500 Euros, going up with eds
support and 4 channel grabbing to ca. 13 000 Euros. No adverdisement,
just a satisfied customer :-)

Best wishes,
Stefan



gul417-at-mail.usask.ca schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All,
}
} I guess this topic has been discussed in this forum (I found that the
} searching function in ListServer achieves was not so straightforward,
} spoiled by Google), but I also think that there should be some newer
} system(s)/technique available.
}
} We have a 25yr-old Philips SEM 505 and are interested in upgrading it
} to digital photography capability. I am looking advice for the system
} should be considered. And estimated cost including computer and
} software (preferably cheaper)?
}
} In addition, does anyone still keep some Polaroid 665 films that we can
} buy before ours run out?
}
} Thank you in advance!
}
} Guosheng Liu
} Dept of Biology,
} Univ of Saskatchewan
} Canada
}
}
} ==============================Original Headers==============================
} 7, 20 -- From gul417-at-mail.usask.ca Fri Jun 5 10:42:37 2009
} 7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55FgaHK016599
} 7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:42:37 -0500
} 7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
} 7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
} 7, 20 -- id {0KKR00601VHZX6-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
} 7, 20 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2009 09:42:36 -0600 (CST)
} 7, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31])
} 7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
} 7, 20 -- with SMTP id {0KKR00IV7VN09I-at-usask.ca} for Microscopy-at-microscopy.com; Fri,
} 7, 20 -- 05 Jun 2009 09:42:36 -0600 (CST)
} 7, 20 -- Date: Fri, 05 Jun 2009 09:42:35 -0600 (CST)
} 7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
} 7, 20 -- Subject: SEM Philips505--Polaroid films and upgrade to digital
} 7, 20 -- To: Microscopy-at-microscopy.com
} 7, 20 -- Message-id: {6016978.1244216555923.JavaMail.gul417-at-mail.usask.ca}
} 7, 20 -- MIME-version: 1.0
} 7, 20 -- Content-type: text/plain; charset=UTF-8
} 7, 20 -- Content-transfer-encoding: 7BIT
} ==============================End of - Headers==============================
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Fri Jun 5 11:03:34 2009
7, 22 -- Received: from mailout09.t-online.de (mailout09.t-online.de [194.25.134.84])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55G3XTU031360
7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 11:03:34 -0500
7, 22 -- Received: from fwd05.aul.t-online.de
7, 22 -- by mailout09.sul.t-online.de with smtp
7, 22 -- id 1MCbtF-0000HV-01; Fri, 05 Jun 2009 18:03:33 +0200
7, 22 -- Received: from mac-pro.local (Eqzd3EZSrheXK1OAQWIwA+zJmkcjHfseubi9SJEDBR-yhCeY7LKilUFgfIyZzAAwbd-at-[91.10.255.87]) by fwd05.aul.t-online.de
7, 22 -- with esmtp id 1MCbt3-21TFaa0; Fri, 5 Jun 2009 18:03:21 +0200
7, 22 -- Message-ID: {4A2941C8.4040406-at-t-online.de}
7, 22 -- Date: Fri, 05 Jun 2009 18:03:20 +0200
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: [Microscopy] SEM Philips505--Polaroid films and upgrade to digital
7, 22 -- References: {200906051547.n55Fl1ia023568-at-ns.microscopy.com}
7, 22 -- In-Reply-To: {200906051547.n55Fl1ia023568-at-ns.microscopy.com}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID: Eqzd3EZSrheXK1OAQWIwA+zJmkcjHfseubi9SJEDBR-yhCeY7LKilUFgfIyZzAAwbd
7, 22 -- X-TOI-MSGID: 512cb042-6443-4fce-b8cb-9e16d4a09485
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 5 Jun 2009 14:42:59 -0500
Subject: [Microscopy] SEM Philips505--Polaroid films and upgrade to digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Guosheng,
Contact Scanservice Corp.

Earl Weltmer
Scanservice Corp.
2681 Dow #D
Tustin, CA 92780
(714) 573-9158
(714) 573-9159 fax
eweltmer-at-sbcglobal.net
http://www.semservice.com

You provide the computer and Scanservice provides the adapters and software.
Passive or active, PCI card or USB, starting at around $3500 US.

Discalimer: Earl and I have worked together for years.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gul417-at-mail.usask.ca [mailto:gul417-at-mail.usask.ca]
Sent: Friday, June 05, 2009 11:45 AM
To: kenconverse-at-qualityimages.biz

Dear All,

I guess this topic has been discussed in this forum (I found that the
searching function in ListServer achieves was not so straightforward,
spoiled by Google), but I also think that there should be some newer
system(s)/technique available.

We have a 25yr-old Philips SEM 505 and are interested in upgrading it
to digital photography capability. I am looking advice for the system
should be considered. And estimated cost including computer and
software (preferably cheaper)?

In addition, does anyone still keep some Polaroid 665 films that we can
buy before ours run out?

Thank you in advance!

Guosheng Liu
Dept of Biology,
Univ of Saskatchewan
Canada


==============================Original Headers==============================
7, 20 -- From gul417-at-mail.usask.ca Fri Jun 5 10:42:37 2009
7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n55FgaHK016599
7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:42:37
-0500
7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- id {0KKR00601VHZX6-at-usask.ca} (original mail from
gul417-at-mail.usask.ca)
7, 20 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2009 09:42:36 -0600
(CST)
7, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31])
7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul
3 2007))
7, 20 -- with SMTP id {0KKR00IV7VN09I-at-usask.ca} for
Microscopy-at-microscopy.com; Fri,
7, 20 -- 05 Jun 2009 09:42:36 -0600 (CST)
7, 20 -- Date: Fri, 05 Jun 2009 09:42:35 -0600 (CST)
7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
7, 20 -- Subject: SEM Philips505--Polaroid films and upgrade to digital
7, 20 -- To: Microscopy-at-microscopy.com
7, 20 -- Message-id: {6016978.1244216555923.JavaMail.gul417-at-mail.usask.ca}
7, 20 -- MIME-version: 1.0
7, 20 -- Content-type: text/plain; charset=UTF-8
7, 20 -- Content-transfer-encoding: 7BIT
==============================End of - Headers==============================




==============================Original Headers==============================
22, 25 -- From kenconverse-at-qualityimages.biz Fri Jun 5 14:42:59 2009
22, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55JgwfH020050
22, 25 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 14:42:58 -0500
22, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
22, 25 -- with ESMTP
22, 25 -- id {20090605194258174.KIOV28090-at-cdptpa-omta04.mail.rr.com} ;
22, 25 -- Fri, 5 Jun 2009 19:42:58 +0000
22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
22, 25 -- To: {gul417-at-mail.usask.ca} , "MSA Listserver" {microscopy-at-microscopy.com}
22, 25 -- Subject: RE: [Microscopy] SEM Philips505--Polaroid films and upgrade to digital
22, 25 -- Date: Fri, 5 Jun 2009 15:42:42 -0400
22, 25 -- Message-ID: {3B8C42A2C004402C920F2A7CA8AA4778-at-Ken}
22, 25 -- MIME-Version: 1.0
22, 25 -- Content-Type: text/plain;
22, 25 -- charset="us-ascii"
22, 25 -- X-Priority: 3 (Normal)
22, 25 -- X-MSMail-Priority: Normal
22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
22, 25 -- Importance: Normal
22, 25 -- Thread-Index: Acnl9JfmkYlUEF18REaMNTor13ko6wABo5iA
22, 25 -- In-Reply-To: {200906051545.n55Fj2XK020565-at-ns.microscopy.com}
22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
22, 25 -- Content-Transfer-Encoding: 8bit
22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55JgwfH020050
==============================End of - Headers==============================




From: takenomm-at-u.washington.edu
Date: Fri, 5 Jun 2009 15:06:34 -0500
Subject: [Microscopy] TEM, LM - compositing / image stitching / making mosaics - Microsoft

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've been experimenting with Microsoft's Image Composite Editor ("ICE") to
make mosaics of microscopy images, and it seems that it works well. Though
most programs such as this are meant to stitch together consumer-level
JPEG photos into a panorama, this particular one can use planar / flat
field TIFFs - which is great for microscopy.

ICE is free of charge but is officially unsupported by Microsoft. It
appears to be stable in my limited testing.

It is available for 32- and 64-bit Windows systems from:

http://research.microsoft.com/en-us/um/redmond/groups/ivm/ICE/

There is an Image Composite Editor forum that has a few support notes
describing the various functions and settings of the program (link at the
bottom of the ICE page):

http://community.research.microsoft.com/forums/112.aspx
http://community.research.microsoft.com/forums/t/2002.aspx

ICE appears to be faster than the MosaicJ plug-in for ImageJ, though the
latter gives you more flexibility and options in layout and is documented
in the scientific literature. I'm not sure which algorithms ICE uses.

http://bigwww.epfl.ch/thevenaz/mosaicj/

usual disclaimers apply (even though I am writing to you from a mere 10
miles away from there:)

--
Marc Takeno, Ph.D. | Research Scientist |
University of Washington Department of Bioengineering |
N330B Foege | Box 355061 (206) 543-4290 | takenomm-at-u.washington.edu


==============================Original Headers==============================
11, 23 -- From takenomm-at-u.washington.edu Fri Jun 5 15:06:34 2009
11, 23 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55K6X6B002838
11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 15:06:34 -0500
11, 23 -- Received: from homer23.u.washington.edu (homer23.u.washington.edu [140.142.12.214])
11, 23 -- by mxout2.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n55K6QCt024953
11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 13:06:32 -0700
11, 23 -- Received: from localhost (takenomm-at-localhost)
11, 23 -- by homer23.u.washington.edu (8.14.3+UW09.03/8.14.3+Submit) with ESMTP id n55K69Q2023948
11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 13:06:09 -0700
11, 23 -- Date: Fri, 5 Jun 2009 13:06:09 -0700 (PDT)
11, 23 -- From: Marc Takeno {takenomm-at-u.washington.edu}
11, 23 -- To: microscopy-at-microscopy.com
11, 23 -- Subject: TEM, LM - compositing / image stitching / making mosaics - Microsoft
11, 23 -- ICE
11, 23 -- Message-ID: {alpine.LRH.2.01.0906051304050.23477-at-homer23.u.washington.edu}
11, 23 -- User-Agent: Alpine 2.01 (LRH 1217 2009-02-23)
11, 23 -- MIME-Version: 1.0
11, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
11, 23 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.6.5.194951
11, 23 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
11, 23 -- BODY_SIZE_1400_1499 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __USER_AGENT 0'
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Fri, 5 Jun 2009 15:24:55 -0500
Subject: [Microscopy] Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Photoshop CS2's Photomerge function works well on my LM flatfield images,
but with TEM images sometimes there are misalignments in some areas. I
assume this is due to spherical aberration in the TEM images. Can any of
the other software options out there detect and correct for spherical
aberration?

Ralph Common
Michigan State University


==============================Original Headers==============================
4, 24 -- From rcommon-at-msu.edu Fri Jun 5 15:24:54 2009
4, 24 -- Received: from sys53.mail.msu.edu (sys53.mail.msu.edu [35.9.75.233])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55KOs5Q017829
4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 15:24:54 -0500
4, 24 -- Received: from [35.9.122.125] (helo=emlab)
4, 24 -- by sys53.mail.msu.edu with esmtpsa (Exim 4.63 #12)
4, 24 -- (TLSv1:RC4-MD5:128)
4, 24 -- id 1MCfyA-0001b1-Gl
4, 24 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2009 16:24:54 -0400
4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu}
4, 24 -- To: {Microscopy-at-microscopy.com}
4, 24 -- Subject: Image stitching
4, 24 -- Date: Fri, 5 Jun 2009 16:24:09 -0400
4, 24 -- Message-ID: {006a01c9e61b$95da0a90$7d7a0923-at-msu.edu}
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: text/plain;
4, 24 -- charset="iso-8859-1"
4, 24 -- Content-Transfer-Encoding: 7bit
4, 24 -- X-Priority: 3 (Normal)
4, 24 -- X-MSMail-Priority: Normal
4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
4, 24 -- Importance: Normal
4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
4, 24 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 5 Jun 2009 16:44:17 -0500
Subject: [Microscopy] Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Photoshop CS2's Photomerge function works well on my LM flatfield images,
} but with TEM images sometimes there are misalignments in some areas. I
} assume this is due to spherical aberration in the TEM images. Can any of
} the other software options out there detect and correct for spherical
} aberration?
}
} Ralph Common
} Michigan State University

In our lab, people now use PS CS4, with - I assume - further improved function(s) for image stitching / merging. At least, the results are very good. - Photomerge.
TEM: this very much depends on the magnification. It works +/- easily and automatically (Photomerge) with images taken in medium / high mag mode (above 3000 x). In low mag mode on our CM12, images contain obvious distortions, preventing perfect alignments. - People who are well trained in PS CS4 can use built-in functions for correcting these distortions, before the images are merged. Not easy, but works satisfactorily.
I just checked PS CS4 again: "Photomerge" even includes filters ("layout") offering some preliminary correction of distortions.
HTH - best regards - Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
7, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jun 5 16:44:17 2009
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55LiGDG001162
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:44:16 -0500
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
7, 26 -- by localhost (Postfix) with SMTP id 3FC06ABFAD
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26 +0200 (CEST)
7, 26 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
7, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 37693ABFD0
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26 +0200 (CEST)
7, 26 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
7, 26 -- with Novell_GroupWise; Fri, 05 Jun 2009 23:44:15 +0200
7, 26 -- Message-Id: {4A29ADC802000054000161F6-at-gwsmtp1.uni-regensburg.de}
7, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
7, 26 -- Date: Fri, 05 Jun 2009 23:44:08 +0200
7, 26 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
7, 26 -- To: {rcommon-at-msu.edu}
7, 26 -- Cc: {Microscopy-at-microscopy.com}
7, 26 -- Subject: Image stitching
7, 26 -- References: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- In-Reply-To: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- Mime-Version: 1.0
7, 26 -- Content-Type: text/plain; charset=US-ASCII
7, 26 -- Content-Disposition: inline
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55LiGDG001162
==============================End of - Headers==============================




From: PWebster-at-hei.org
Date: Fri, 5 Jun 2009 16:52:03 -0500
Subject: [Microscopy] EM: gold and HRP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I wonder if anyone has performed pre-embedding colocalization with two antibodies, labeling one with nano-gold and the other with an HRP-conjugated antibody?

Specifically, I would like to know how to apply the two markers. are the markers both applied and then the visualization reactions performed, or are each of the markers applied and reacted individually?

Would the silver enhancement of the gold, cause a reaction with the DAB reaction product?

I guess I could find all this out for myself and publish it in an unknown journal, but it would be great if there was already a protocol available.

My apologies to the many people who have no idea what I am asking. I targeted the question to the few that may know the answer.

Maybe there are some wise people at Aurion or Nanoprobes who have the answer (cheers to Rick and Jan!).

Regards,

Paul Webster

House Ear Institute
2100 W 3rd St
Los Angeles
CA 90057.

Today it rained


==============================Original Headers==============================
13, 20 -- From PWebster-at-hei.org Fri Jun 5 16:52:03 2009
13, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55Lq3Hk014940
13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:52:03 -0500
13, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 20 -- Content-class: urn:content-classes:message
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain;
13, 20 -- charset="iso-8859-1"
13, 20 -- Subject: EM: gold and HRP
13, 20 -- Date: Fri, 5 Jun 2009 14:52:02 -0700
13, 20 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F830814CF98-at-hi0sml1.hei.org}
13, 20 -- X-MS-Has-Attach:
13, 20 -- X-MS-TNEF-Correlator:
13, 20 -- Thread-Topic: gold and HRP
13, 20 -- Thread-Index: AcnmJ9vjuLGo6Nn1S7664zZBFybxRQ==
13, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
13, 20 -- To: {microscopy-at-microscopy.com}
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55Lq3Hk014940
==============================End of - Headers==============================




From: ech-at-uvic.ca
Date: Fri, 5 Jun 2009 18:07:39 -0500
Subject: [Microscopy] viaWWW: Lighting of a Facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ech-at-uvic.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ech-at-uvic.ca
Name: Elaine Humphrey

Organization: University of Victoria

Title-Subject: [Filtered] Lighting of a Facility

Question: Has anyone got any advice about the lighting for an
electron microscope facility in the basement of a building?

In the winter when you leave home in the dark, leave work in the
dark, and actually work long hours in rooms with low lighting, I
think the lighting especially in the corridor and outside rooms can
have a profound effect on the mood of the people working there.

We are building a new em facility in the basement of a Science
building. I am given to understand that the lighting will be a
standard 3500 rating. Is this enough?
Many thanks
Elaine

Dr. Elaine Humphrey
STEHM technologist and lab manager
Advanced Microscopy Facility
University of Victoria
Canada

Login Host: 142.104.215.8
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri Jun 5 18:07:39 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55N7b0f009678
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 18:07:38 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c64f5595c3ff-at-[206.69.208.22]}
9, 11 -- Date: Fri, 5 Jun 2009 18:07:37 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: ech-at-uvic.ca (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Lighting of a Facility
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: elizabeth-at-aaisolutions.com
Date: Fri, 5 Jun 2009 18:08:29 -0500
Subject: [Microscopy] viaWWW: Nicolet Almega XR Dispersive Raman Spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both elizabeth-at-aaisolutions.com as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: elizabeth-at-aaisolutions.com
Name: Liz Leigh

Organization: Alliance Analytical

Title-Subject: [Filtered] Nicolet Almega XR Dispersive Raman Spectrometer

Question: My company recently acquired a 2004
Thermo Nicolet Almega XR Dispersive Raman
Spectrometer. Itís a complete system in very
good condition. Weíre resellers but generally
donít deal with this type of equipment so I am a
bit lost as to who would use this. Also, any
suggestions/knowledge of this instrument is much
appreciated.

Login Host: 64.81.17.181
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Fri Jun 5 18:08:29 2009
7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55N8Sm5010881
7, 13 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 18:08:29 -0500
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240803c64f55c1ce66-at-[206.69.208.22]}
7, 13 -- Date: Fri, 5 Jun 2009 18:08:27 -0500
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: elizabeth-at-aaisolutions.com (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: Nicolet Almega XR Dispersive Raman Spectrometer
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55N8Sm5010881
==============================End of - Headers==============================




From: sekkio-at-mac.com
Date: Sat, 6 Jun 2009 02:03:48 -0500
Subject: [Microscopy] alby

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends,
please do not miss the Fluorescence School in Genoa this Summer!
Check on the Fluorescence Foundation webpage http://www.fluorescence-foundation.org/
See you in Genoa
All the best
Alby

----------------------------------------
Alberto Diaspro
Head, Nanophysics Unit
Senior Scientist
The Italian Institute of Technology -IIT
Via Morego, 30
16163 - Genova (Italy)
phone: +39 010 71781503
fax: +39 010 720321
http://www.iit.it
alberto.diaspro-at-iit.it

Professor of Applied Physics
Department of Physics
University of Genova
Via Dodecaneso, 33
16146 Genova - Italy
tel. +39 010 353 6426
fax. +39 010 314218
http://www.lambs.it
diaspro-at-fisica.unige.it
-------------------------------------------------------


==============================Original Headers==============================
4, 19 -- From sekkio-at-mac.com Sat Jun 6 02:03:48 2009
4, 19 -- Received: from asmtpout024.mac.com (asmtpout024.mac.com [17.148.16.99])
4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5673lZN017736
4, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 6 Jun 2009 02:03:48 -0500
4, 19 -- MIME-version: 1.0
4, 19 -- Content-transfer-encoding: 7BIT
4, 19 -- Content-type: text/plain; charset=US-ASCII; format=flowed
4, 19 -- Received: from [10.0.1.3]
4, 19 -- (host10-59-dynamic.60-82-r.retail.telecomitalia.it [82.60.59.10])
4, 19 -- by asmtp024.mac.com
4, 19 -- (Sun Java(tm) System Messaging Server 6.3-8.01 (built Dec 16 2008; 32bit))
4, 19 -- with ESMTPSA id {0KKT005TQ29C8C50-at-asmtp024.mac.com} for
4, 19 -- Microscopy-at-microscopy.com; Sat, 06 Jun 2009 00:03:47 -0700 (PDT)
4, 19 -- Message-id: {6522D8B4-3BF3-4B89-8C00-15B49EBE5B76-at-mac.com}
4, 19 -- From: Alberto Diaspro {sekkio-at-mac.com}
4, 19 -- To: Microscopy-at-microscopy.com
4, 19 -- Subject: alby
4, 19 -- Date: Sat, 06 Jun 2009 09:03:46 +0200
4, 19 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: Aleksandr.Mironov-at-manchester.ac.uk
Date: Mon, 8 Jun 2009 10:02:00 -0500
Subject: [Microscopy] Re: EM: gold and HRP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Paul,

Probably, the best person to ask is Roman Polishchuk from Mario Negri
Sud (polish-at-negrisud.it {mailto:polish-at-negrisud.it} ). He applied HRP,
nanogold and GoldEnhance quite regularly. As far as I remember you apply
at first all the antibodies, then develop HRP, then developNanogold with
GoldEnhance.

Sincerely,
Alex


} Hello,
}
} I wonder if anyone has performed pre-embedding colocalization with two antibodies, labeling one with nano-gold and the other with an HRP-conjugated antibody?
}
} Specifically, I would like to know how to apply the two markers. are the markers both applied and then the visualization reactions performed, or are each of the markers applied and reacted individually?
}
} Would the silver enhancement of the gold, cause a reaction with the DAB reaction product?
}
} I guess I could find all this out for myself and publish it in an unknown journal, but it would be great if there was already a protocol available.
}
} My apologies to the many people who have no idea what I am asking. I targeted the question to the few that may know the answer.
}
} Maybe there are some wise people at Aurion or Nanoprobes who have the answer (cheers to Rick and Jan!).
}
} Regards,
}
} Paul Webster
}
} House Ear Institute
} 2100 W 3rd St
} Los Angeles
} CA 90057.
}
} Today it rained
} ========================End of - Headers==============================
}

--
Dr. Aleksandr Mironov MD, PhD
Experimental Officer
D.1527, M.Smith Building
EM Core Facility, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-5645
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
http://www.ls.manchester.ac.uk/research/facilities/electronmicroscopy/


==============================Original Headers==============================
8, 28 -- From Aleksandr.Mironov-at-manchester.ac.uk Mon Jun 8 10:01:59 2009
8, 28 -- Received: from tranquility.mcc.ac.uk (tranquility.mcc.ac.uk [130.88.200.145])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n58F1wfV004606
8, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Jun 2009 10:01:59 -0500
8, 28 -- Received: from gerhayn.mcc.ac.uk ([10.2.18.1])
8, 28 -- by tranquility.mcc.ac.uk with esmtps (TLSv1:AES256-SHA:256)
8, 28 -- (Exim 4.69 (FreeBSD))
8, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} )
8, 28 -- id 1MDgMH-0008fE-Bj; Mon, 08 Jun 2009 16:01:57 +0100
8, 28 -- Received: from mi-amironov.smith.man.ac.uk ([130.88.210.224]:3212)
8, 28 -- by gerhayn.mcc.ac.uk with esmtpsa (TLSv1:AES256-SHA:256)
8, 28 -- (Exim 4.69 (FreeBSD))
8, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} )
8, 28 -- id 1MDgMH-00075B-8t; Mon, 08 Jun 2009 16:01:57 +0100
8, 28 -- Message-ID: {4A2D36AE.4050600-at-manchester.ac.uk}
8, 28 -- Date: Mon, 08 Jun 2009 16:05:02 +0000
8, 28 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk}
8, 28 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
8, 28 -- MIME-Version: 1.0
8, 28 -- To: PWebster-at-hei.org, Microscopy-at-microscopy.com
8, 28 -- Subject: Re: [Microscopy] EM: gold and HRP
8, 28 -- References: {200906052155.n55LtDnh023575-at-ns.microscopy.com}
8, 28 -- In-Reply-To: {200906052155.n55LtDnh023575-at-ns.microscopy.com}
8, 28 -- Content-Type: text/plain; charset=windows-1251; format=flowed
8, 28 -- Content-Transfer-Encoding: 7bit
8, 28 -- X-Authenticated-Sender: Aleksandr Mironov from mi-amironov.smith.man.ac.uk [130.88.210.224]:3212
8, 28 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk
8, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details.
==============================End of - Headers==============================




From: gul417-at-mail.usask.ca
Date: Mon, 8 Jun 2009 10:16:31 -0500
Subject: [Microscopy] thank you for the feedbacks on upgading SEM from Polariod to digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I received tremendous responses in regard to upgrading our SEM from Polaroid to digital imaging.

Many thanks for all your very useful feedback and advice on my post. I'll not reply to each of the responses but might contact some of you if I need further info.

Thank you again!

Cheers,

Guosheng


==============================Original Headers==============================
7, 20 -- From gul417-at-mail.usask.ca Mon Jun 8 10:16:30 2009
7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n58FGTO8018983
7, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Jun 2009 10:16:30 -0500
7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- id {0KKX00H01E3PSJ-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
7, 20 -- for Microscopy-at-microscopy.com; Mon, 08 Jun 2009 09:16:29 -0600 (CST)
7, 20 -- Received: from paws3.usask.ca (paws3.usask.ca [128.233.194.31])
7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- with SMTP id {0KKX00DEFEFFT7-at-usask.ca} for Microscopy-at-microscopy.com; Mon,
7, 20 -- 08 Jun 2009 09:16:27 -0600 (CST)
7, 20 -- Date: Mon, 08 Jun 2009 09:16:26 -0600 (CST)
7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
7, 20 -- Subject: thank you for the feedbacks on upgading SEM from Polariod to digital
7, 20 -- To: Microscopy-at-microscopy.com
7, 20 -- Message-id: {9607089.1244474186982.JavaMail.gul417-at-mail.usask.ca}
7, 20 -- MIME-version: 1.0
7, 20 -- Content-type: text/plain; charset=UTF-8
7, 20 -- Content-transfer-encoding: 7BIT
==============================End of - Headers==============================




From: hyi-at-emory.edu
Date: Mon, 8 Jun 2009 12:32:54 -0500
Subject: [Microscopy] EM: gold and HRP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Paul:

This method is widely used for EM dual labeling in brain tissue. The general procedure involves incubation with two primaries (if from different species), incubation of two secondaries (one conjugated to HRP, the other Au), DAB reaction, post-fixation with glutaraldehyde, and finally silver enhancement. You can also use ABC system for DAB labeling.

According to some, DAB reaction product can mask the Fab sites, therefore the system can also be applied when two primaries are from the same species. In this case, two labelings should be done sequentially with the labeling using HRP going first. Presumably, any free Fab from the first secondary antibody would no longer be available for binding the second primary antibody after DAB reaction. Anyway, I have never tried this myself. I like to play safe by using two different primaries.

Hope this helps. Let me know if you want to have PDFs. Thank you.

Hong

________________________________________
X-from: PWebster-at-hei.org [PWebster-at-hei.org]
Sent: Friday, June 05, 2009 5:53 PM
To: Yi, Hong


Hello,

I wonder if anyone has performed pre-embedding colocalization with two antibodies, labeling one with nano-gold and the other with an HRP-conjugated antibody?

Specifically, I would like to know how to apply the two markers. are the markers both applied and then the visualization reactions performed, or are each of the markers applied and reacted individually?

Would the silver enhancement of the gold, cause a reaction with the DAB reaction product?

I guess I could find all this out for myself and publish it in an unknown journal, but it would be great if there was already a protocol available.

My apologies to the many people who have no idea what I am asking. I targeted the question to the few that may know the answer.

Maybe there are some wise people at Aurion or Nanoprobes who have the answer (cheers to Rick and Jan!).

Regards,

Paul Webster

House Ear Institute
2100 W 3rd St
Los Angeles
CA 90057.

Today it rained


==============================Original Headers==============================
13, 20 -- From PWebster-at-hei.org Fri Jun 5 16:52:03 2009
13, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55Lq3Hk014940
13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:52:03 -0500
13, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 20 -- Content-class: urn:content-classes:message
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain;
13, 20 -- charset="iso-8859-1"
13, 20 -- Subject: EM: gold and HRP
13, 20 -- Date: Fri, 5 Jun 2009 14:52:02 -0700
13, 20 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F830814CF98-at-hi0sml1.hei.org}
13, 20 -- X-MS-Has-Attach:
13, 20 -- X-MS-TNEF-Correlator:
13, 20 -- Thread-Topic: gold and HRP
13, 20 -- Thread-Index: AcnmJ9vjuLGo6Nn1S7664zZBFybxRQ==
13, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
13, 20 -- To: {microscopy-at-microscopy.com}
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55Lq3Hk014940
==============================End of - Headers==============================

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).


==============================Original Headers==============================
24, 34 -- From hyi-at-emory.edu Mon Jun 8 12:32:54 2009
24, 34 -- Received: from mr1.cc.emory.edu (mr1.cc.emory.edu [170.140.52.90])
24, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n58HWsNa006797
24, 34 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2009 12:32:54 -0500
24, 34 -- Received: from EXCHEDGE1.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254])
24, 34 -- by mr1.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n58HWjg0028206
24, 34 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2009 13:32:45 -0400
24, 34 -- Received: from EXCHHUB1.Enterprise.emory.net (170.140.30.53) by
24, 34 -- EXCHEDGE1.enterprise.emory.net (170.140.52.33) with Microsoft SMTP Server
24, 34 -- (TLS) id 8.2.148.1; Mon, 8 Jun 2009 13:32:51 -0400
24, 34 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by
24, 34 -- EXCHHUB1.Enterprise.emory.net ([170.140.30.53]) with mapi; Mon, 8 Jun 2009
24, 34 -- 13:32:45 -0400
24, 34 -- From: "Yi, Hong" {hyi-at-emory.edu}
24, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
24, 34 -- Date: Mon, 8 Jun 2009 13:31:50 -0400
24, 34 -- Subject: RE: [Microscopy] EM: gold and HRP
24, 34 -- Thread-Topic: [Microscopy] EM: gold and HRP
24, 34 -- Thread-Index: AcnmKBgwtMpZmEhES22XUfRh6PuT4wCNulg2
24, 34 -- Message-ID: {3940C1839888A54DAE85AE20F294FB69010CFB1C5058-at-EXCHANGE12.Enterprise.emory.net}
24, 34 -- References: {200906052153.n55LraBk019235-at-ns.microscopy.com}
24, 34 -- In-Reply-To: {200906052153.n55LraBk019235-at-ns.microscopy.com}
24, 34 -- Accept-Language: en-US
24, 34 -- Content-Language: en-US
24, 34 -- X-MS-Has-Attach:
24, 34 -- X-MS-TNEF-Correlator:
24, 34 -- acceptlanguage: en-US
24, 34 -- Content-Type: text/plain; charset="us-ascii"
24, 34 -- MIME-Version: 1.0
24, 34 -- X-emory.edu-MailScanner: Found to be clean
24, 34 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu
24, 34 -- X-Spam-Status: No
24, 34 -- Content-Transfer-Encoding: 8bit
24, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n58HWsNa006797
==============================End of - Headers==============================




From: swatkins-at-pitt.edu
Date: Mon, 8 Jun 2009 14:46:16 -0500
Subject: [Microscopy] leica cryokits available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone. due to the benevolence of the National Center for Research Resources we are able to replace two of our cryokits. We would like to donate these to anyone who has a genuine need. However, they can only be considered usable for parts, we have 2 FC4Ds and one FCS2, these are chambers and controllers, and some pump stuff etc. These devices are no longer made and you cannot get any parts new to repair them. However, they would be very valuable to a group who would need them to maintain devices they already have.
Let us know what you need. (we will spread them as thin as we can).
Simon



Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu


==============================Original Headers==============================
5, 23 -- From swatkins-at-pitt.edu Mon Jun 8 14:46:16 2009
5, 23 -- Received: from pitt-ht-01.univ.pitt.edu (pitt-ht-01.cssd.pitt.edu [136.142.251.58])
5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n58JkFFG028591
5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2009 14:46:16 -0500
5, 23 -- Received: from PITT-EXCH-07.univ.pitt.edu ([136.142.251.52]) by
5, 23 -- pitt-ht-01.univ.pitt.edu ([136.142.251.58]) with mapi; Mon, 8 Jun 2009
5, 23 -- 15:46:16 -0400
5, 23 -- From: "Watkins, Simon C" {swatkins-at-pitt.edu}
5, 23 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
5, 23 -- Date: Mon, 8 Jun 2009 15:46:15 -0400
5, 23 -- Subject: leica cryokits available
5, 23 -- Thread-Topic: leica cryokits available
5, 23 -- Thread-Index: AQHJ6HHIEvtckTitm0CeFgDN9awnBg==
5, 23 -- Message-ID: {5DB6E1A3283C6B4984EA0EADAEC583F484918E3B03-at-PITT-EXCH-07.univ.pitt.edu}
5, 23 -- Accept-Language: en-US
5, 23 -- Content-Language: en-US
5, 23 -- X-MS-Has-Attach:
5, 23 -- X-MS-TNEF-Correlator:
5, 23 -- acceptlanguage: en-US
5, 23 -- Content-Type: text/plain; charset="us-ascii"
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Transfer-Encoding: 8bit
5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n58JkFFG028591
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 9 Jun 2009 05:04:13 -0500
Subject: [Microscopy] viaWWW: double images in microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In my case the double vision is totally dependent on the orientation of my
head [with eyes looking forward]. I wonder if adjusting the chair position
and the microscope binoculars' eyepiece focus correctly* might help you
accommodate more easily. Gregg seems to be more in tune with the particular
problem having experienced it himself.

Keith

*Not that I need much in the way of adjustable binocular eyepieces [other
than to set them 'parfocal' with the camera], but a few tips nicked from the
net:

"Binocular vision is much more sensitive to light and detail than monocular
vision, so if you have a binocular microscope, take advantage of it. One or
both of the eyepieces may be a telescoping eyepiece, that is, you can focus
it. Since very few people have eyes that are perfectly matched, most of us
need to focus one eyepiece to match the other image. Look with the
appropriate eye into the fixed eyepiece and focus with the microscope focus
knob. Next, look into the adjustable eyepiece (with the other eye of
course), and adjust the eyepiece, not the microscope."

"If only one eyepiece is adjustable: To adjust these lenses, first, cover
the adjustable lens (or your eye) so that you cannot see the image through
it. Next, focus the microscope the way you normally would so that a sharp
image is produced through the ocular lens. Cover this lens (or eye) and view
the image through the other (adjustable) ocular. Turn the ocular lens but
not the focus knob and adjust so that the image is sharp."

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: greggps-at-umich.edu [mailto:greggps-at-umich.edu]
Sent: 05 June 2009 15:36
To: kjmorris-at-well.ox.ac.uk

Ron,
I always had double vision when using binocular eyepieces on binoculars as a
child and through college, and never needed or wore glasses. For my first
job in EM after college, I still had double-vision when using binocular
microscopes. After 3-5 months of sectioning on the ultramicrotome and
favoring one eye, I was looking at a semithin section on a basic binocular
compound microscope one day, and the two images slowly started moving
together until they overlapped. I didn't move at all during this 1-2 second
experience. (It probably only seemed that long, though.) I have been able to
use binocular microscopes correctly ever since.

My theory is that my brain just needed to adjust to this uncommon phenomenon
of binocular eyepieces, and merely took longer than the average person.

As a follow-up: When I got eyeglasses a few years afterwards, I had a
terrible time adjusting to every new prescription for several years. Now the
adjustment is very brief.

Good luck to your student. Advise them to stick with it, and it may correct
itself.

Regards,
~Gregg

Gregg Sobocinski
Imaging Specialist/Microscopist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

"We are all individuals!" -- Monty Python, The Life of Brian. (The quote
seems appropriate here.)



-----Original Message-----
X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk]
Sent: Friday, June 05, 2009 5:46 AM
To: Sobocinski, Gregg

I have 'double vision', although this has been minimised with corrective
surgery a few years ago [my 'bad' right eye had drifted away from that of my
childhood and my brain was starting to process the visual info again from it
- so they put the eye pointing inwards again to switch it 'off', as the
brain was used to ignoring it there].

All this was from a lazy eye [squint] at childhood though and a clear visual
defect. In fact I keep the microscope binoculars open too wide so I only
look down with the left [good] eye* - I shift the good eye from right to
left binocular during cleaning for the users. However my lazy eye really
counts as a major visual problem and apparently your user hasn't got
anything like that [my right eyes perfect just the brain doesn't process the
info as it didn't like the double vision either when growing during my
youth]. Visual/cortex problems can produce odd effects - there's one where
people think they see little grey people...

I expect straining to peer down the microscope is knocking the eye out of 3D
alignment [you can make the eyes squint inwards if you try - personally I
don't], but perhaps someone from optimetrics can advise. Try the monocular
approach as well.

Keith

*I don't suppose many remember the monocular microscopes of yore, except
perhaps from school, but there's no stereoscopic info on a compound
microscope so monocular is fine. Not that I'd see 3D information anyway - in
fact we make good photographers, probably because we have an eye for the 2D
scene.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: richard.ross-at-allisontransmission.com
[mailto:richard.ross-at-allisontransmission.com]
Sent: 05 June 2009 00:31
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both richard.ross-at-allisontransmission.com as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: richard.ross-at-allisontransmission.com
Name: Richard Ross

Organization: Allison Transmission Inc.

Title-Subject: [Microscopy] viaWWW: double images in microscope

Question: Could this person be one of the 10-15% of the population
that does not have binocular vision? I am one of these, and my
less-dominant eye is ignored for my central vision, so no microscope
issues (other than no stereo 3-d capability!). Perhaps this
individual's visual cortex is develeoped somewhere between binocular
and monocular vision and has trouble fusing the view presented in a
microscope. Just some thoughts to consider.



Login Host: 12.53.132.4
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 4 18:24:49 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n54NOl8r014610
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 18:24:48
-0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c64e082dcbc0-at-[206.69.208.22]}
8, 11 -- Date: Thu, 4 Jun 2009 18:24:46 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: richard.ross-at-allisontransmission.com (by way of
MicroscopyListserver)
8, 11 -- Subject: viaWWW: double images in microscope
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
20, 22 -- From kjmorris-at-well.ox.ac.uk Fri Jun 5 04:39:19 2009
20, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk
[129.67.44.2])
20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n559dJe8027496
20, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 5 Jun 2009 04:39:19
-0500
20, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178]
helo=CytoWhizz)
20, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
20, 22 -- id 1MCVtO-00024X-GL
20, 22 -- for Microscopy-at-Microscopy.Com; Fri, 05 Jun 2009 10:39:18
+0100
20, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
20, 22 -- To: {Microscopy-at-Microscopy.Com}
20, 22 -- References: {200906042331.n54NVMor024949-at-ns.microscopy.com}
20, 22 -- Subject: RE: [Microscopy] viaWWW: double images in microscope
20, 22 -- Date: Fri, 5 Jun 2009 10:39:18 +0100
20, 22 -- Message-ID: {33EBE5989E74400F8D4CD698B44D3BA3-at-CytoWhizz}
20, 22 -- MIME-Version: 1.0
20, 22 -- Content-Type: text/plain;
20, 22 -- charset="us-ascii"
20, 22 -- Content-Transfer-Encoding: 7bit
20, 22 -- X-Mailer: Microsoft Office Outlook 11
20, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 22 -- Thread-Index: AcnlbJJFtu1n4AzzQCe0EwvkdkdpPAAUdqgg
20, 22 -- In-Reply-To: {200906042331.n54NVMor024949-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
35, 26 -- From greggps-at-umich.edu Fri Jun 5 09:30:46 2009
35, 26 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu
(itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201])
35, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n55EUkfJ031912
35, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 09:30:46
-0500
35, 26 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu
([141.211.3.235]) by
35, 26 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi;
Fri, 5 Jun
35, 26 -- 2009 10:30:46 -0400
35, 26 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
35, 26 -- To: Microscopy MSA {Microscopy-at-microscopy.com} ,
35, 26 -- "RonMervis-at-neurostructural.org"
{RonMervis-at-neurostructural.org}
35, 26 -- Date: Fri, 5 Jun 2009 10:30:45 -0400
35, 26 -- Subject: RE: [Microscopy] double images in microscopes-My own
happy ending
35, 26 -- Thread-Topic: [Microscopy] double images in microscopes-My own
happy ending
35, 26 -- Thread-Index: Acnlwmui20ZGUzIXRleF4jqQNmgjGwAJXTaQ
35, 26 -- Message-ID:
{9F8ADD9ABC7F264E82EDDE4C10DA393406143A68FE-at-ITCS-ECLS-1-VS3.adsroot.itcs.umi
ch.edu}
35, 26 -- References: {200906050945.n559jrep006946-at-ns.microscopy.com}
35, 26 -- In-Reply-To: {200906050945.n559jrep006946-at-ns.microscopy.com}
35, 26 -- Accept-Language: en-US
35, 26 -- Content-Language: en-US
35, 26 -- X-MS-Has-Attach:
35, 26 -- X-MS-TNEF-Correlator:
35, 26 -- acceptlanguage: en-US
35, 26 -- Content-Type: text/plain; charset="us-ascii"
35, 26 -- MIME-Version: 1.0
35, 26 -- Content-Transfer-Encoding: 8bit
35, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n55EUkfJ031912
==============================End of - Headers==============================


==============================Original Headers==============================
47, 22 -- From kjmorris-at-well.ox.ac.uk Tue Jun 9 05:04:13 2009
47, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
47, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n59A4Crg031427
47, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 9 Jun 2009 05:04:12 -0500
47, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
47, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
47, 22 -- id 1MDyBf-0000BU-E7
47, 22 -- for Microscopy-at-Microscopy.Com; Tue, 09 Jun 2009 11:04:12 +0100
47, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
47, 22 -- To: {Microscopy-at-Microscopy.Com}
47, 22 -- References: {200906051436.n55Ea21u009773-at-ns.microscopy.com}
47, 22 -- Subject: RE: [Microscopy] RE: double images in microscopes
47, 22 -- Date: Tue, 9 Jun 2009 11:04:24 +0100
47, 22 -- Message-ID: {CD6826AA8D284501AA62F939A153F246-at-CytoWhizz}
47, 22 -- MIME-Version: 1.0
47, 22 -- Content-Type: text/plain;
47, 22 -- charset="us-ascii"
47, 22 -- Content-Transfer-Encoding: 7bit
47, 22 -- X-Mailer: Microsoft Office Outlook 11
47, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
47, 22 -- thread-index: Acnl6vREHfGqK8j8S62oxuImSxn5FgCLLKkg
47, 22 -- In-Reply-To: {200906051436.n55Ea21u009773-at-ns.microscopy.com}
==============================End of - Headers==============================




From: i.ross-at-shef.ac.uk
Date: Tue, 9 Jun 2009 07:42:03 -0500
Subject: [Microscopy] viaWWW: Conversion of FEI .ser and .emi raw data to digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both i.ross-at-shef.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: i.ross-at-shef.ac.uk
Name: Ian Ross

Organization: University of Sheffield

Title-Subject: [Filtered] Conversion of FEI .ser and .emi raw data to
digital microsgraph

Question: I have some Image and EELS Spectra data for analysis
acquired on an FEI instrument elsewhere. The data is in the format of
a series of .ser or .emi files. I would be very grateful for any
assistance as to how to convert these files to a format that can be
read into Digital Micrograph for further analysis.


Login Host: 79.68.96.237
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Tue Jun 9 07:42:03 2009
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n59Cg3lH016809
7, 12 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 07:42:03 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- Message-Id: {p06240800c6540906d81d-at-[206.69.208.22]}
7, 12 -- Date: Tue, 9 Jun 2009 07:42:02 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: i.ross-at-shef.ac.uk (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Conversion of FEI .ser and .emi raw data to digital
7, 12 -- microsgraph
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Tue, 9 Jun 2009 09:50:22 -0500
Subject: [Microscopy] EM: Sputter coater advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, fellow listers,

Recent experience has taught us that we need to have a small in-house sputter coater. (Many, many thanks to Joyce Miller at MTSU's MIMIC facility for her generous and patient help with our problem). Since these don't look to be hugely expensive, and since we'll likely only ever be allowed to buy one, I thought we'd be best off by aiming for the most versatile (various metals and carbon?), most fool-proof, and most reliable coater out there. I'm hoping that the folks on the list will be able to make some recommendations?  (Commercial companies are welcome to contact me off-list, but please don't clutter the list with responses)

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

The opinion(s) expressed in this message are those of the sender only. This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.


This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
8, 31 -- From Robert.Zonis-at-Sanford.com Tue Jun 9 09:50:21 2009
8, 31 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243])
8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n59EoJMI001186
8, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 09:50:20 -0500
8, 31 -- X-VirusChecked: Checked
8, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
8, 31 -- X-Msg-Ref: server-8.tower-192.messagelabs.com!1244558814!5743830!20
8, 31 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,-
8, 31 -- X-Originating-IP: [198.176.16.25]
8, 31 -- Received: (qmail 22797 invoked from network); 9 Jun 2009 14:47:18 -0000
8, 31 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
8, 31 -- by server-8.tower-192.messagelabs.com with SMTP; 9 Jun 2009 14:47:18 -0000
8, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
8, 31 -- Tue, 9 Jun 2009 09:47:15 -0500
8, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 31 -- Content-class: urn:content-classes:message
8, 31 -- MIME-Version: 1.0
8, 31 -- Content-Type: text/plain;
8, 31 -- charset="iso-8859-1"
8, 31 -- Subject: [Microscopy] EM: Sputter coater advice
8, 31 -- Date: Tue, 9 Jun 2009 09:45:56 -0500
8, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9403079033-at-naoaksasebe02.nr.ad.newellco.com}
8, 31 -- X-MS-Has-Attach:
8, 31 -- X-MS-TNEF-Correlator:
8, 31 -- Thread-Topic: [Microscopy] EM: Sputter coater advice
8, 31 -- thread-index: AcnpDNdqThZUhRFeQKii50V45BY6iwAA74Cg
8, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
8, 31 -- To: {Microscopy-at-microscopy.com}
8, 31 -- X-OriginalArrivalTime: 09 Jun 2009 14:47:15.0649 (UTC) FILETIME=[2E40E310:01C9E911]
8, 31 -- Content-Transfer-Encoding: 8bit
8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n59EoJMI001186
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Tue, 9 Jun 2009 13:11:20 -0500
Subject: [Microscopy] Electron Microscopy Director Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,

At Oregon State University we are currently searching for a
director for our Electron Microscopy Facility. The link to the posting
is found here:

https://jobs.oregonstate.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1244046446800

Basically it is a non-tenure track position, initially it is set up as
a 75% time funded position to run the facility, and the additional 25%
time could be filled if he/she pursues external grant money for
research. So it's essentially a research faculty position with 75%
hard money guaranteed.

Please forward to anyone you know who might be interested.

Best regards,

Jay


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Jamie Kruzic
Associate Professor
Oregon State University
School of Mechanical, Industrial, and
Manufacturing Engineering
204 Rogers Hall
Corvallis, OR 97331
jamie.kruzic-at-oregonstate.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




==============================Original Headers==============================
12, 20 -- From donovan-at-uoregon.edu Tue Jun 9 13:11:19 2009
12, 20 -- Received: from smtp.uoregon.edu (mserv3.uoregon.edu [128.223.142.101])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n59IBIDJ022119
12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 13:11:18 -0500
12, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
12, 20 -- (authenticated bits=0)
12, 20 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n59IBFvx013596
12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
12, 20 -- Tue, 9 Jun 2009 11:11:15 -0700
12, 20 -- Message-Id: {200906091811.n59IBFvx013596-at-smtp.uoregon.edu}
12, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 20 -- Date: Tue, 09 Jun 2009 11:11:54 -0700
12, 20 -- To: microscopy-at-microscopy.com
12, 20 -- From: John Donovan {donovan-at-uoregon.edu}
12, 20 -- Subject: Electron Microscopy Director Opening
12, 20 -- Cc: kruzicj-at-engr.orst.edu
12, 20 -- Mime-Version: 1.0
12, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
12, 20 -- X-Virus-Scanned: ClamAV 0.94.2/9445/Tue Jun 9 07:42:26 2009 on mserv3
12, 20 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: dave-at-boeckeler.com
Date: Tue, 9 Jun 2009 14:17:39 -0500
Subject: [Microscopy] High Pressure Freezer User Group Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

If you are planning on attending the MC2009 Congress in Graz, Austria,
this September, and if you have an interest in High Pressure Freezing,
please consider attending the following user group meeting:

High Pressure Freezer User Group Meeting

Wednesday, September 2, 2009
5:00pm until around 7:00pm
MC2009
Congress Hall Graz
Albrechtgasse 1, A-8010 Graz, Austria

Meeting Philosophy: An informal, relaxed, discussion group, sharing
experiences with high pressure freezing of biological samples. Although
this meeting is primarily focused on experiences with the HPM-010 High
Pressure Freezing Machine, it is open to anyone with an interest in
cryoimmobilization by high pressure freezing or with experiences to
share using other freezing devices.

Meeting Chairperson: Andres Kaech, PhD
Center for Microscopy and Image Analysis
University of Zurich
Meeting Agenda:

5:00pm Welcome & Introduction: A brief history of the
HPM-010 High Pressure Freezing Machine and the
birth of high pressure freezing in Zurich

5:15pm High Pressure Freezing of in vivo-DAB stained
organelles
Prof. Dr. Adolf Ellinger
Medical University of Vienna, Austria

5:45pm High Pressure Freezing of cells: the good, the
bad and the ugly.
Dr. Carolina Machado
NIBSC, Potters Bar, UK

6:00pm Open Discussion for approximately 1 hour

7:00pm (open) Meeting closes


Please drop me an e-mail if you would like to reserve a place.

See you in Graz

Dave Roberts
Director-RMC Products
Boeckeler Instruments Inc
4650 S. Butterfield Drive
Tucson, Arizona 85714
Tel: 520-745-0001
Fax: 520-745-0004
www.rmcproducts.com
dave-at-boeckeler.com

==============================Original Headers==============================
15, 15 -- From dave-at-boeckeler.com Tue Jun 9 14:17:39 2009
15, 15 -- Received: from spamstopper.cnsusa.com (spamstopper.cnsusa.com [209.104.7.157])
15, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n59JHdp7011922
15, 15 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 14:17:39 -0500
15, 15 -- Received: from [10.0.1.209] [207.224.156.250] by spamstopper.cnsusa.com with ESMTP
15, 15 -- (SMTPD-10.01) id A5520378; Tue, 09 Jun 2009 12:17:38 -0700
15, 15 -- Message-ID: {4A2EB551.2000708-at-boeckeler.com}
15, 15 -- Date: Tue, 09 Jun 2009 12:17:37 -0700
15, 15 -- From: Dave Roberts {dave-at-boeckeler.com}
15, 15 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
15, 15 -- MIME-Version: 1.0
15, 15 -- To: microscopy-at-microscopy.com
15, 15 -- Subject: High Pressure Freezer User Group Meeting
15, 15 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
15, 15 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 9 Jun 2009 15:58:07 -0500
Subject: [Microscopy] 3D software for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers,

We are on the market for 3D software for SEM (stereo measurements,
visualization, etc.) I would greatly appreciate all recommendations on
existing packages. Also I would like to find out if these packages are
able to recognize tilt axes (it looks like time consuming task to orient
images exactly along tilt axes.)

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
8, 23 -- From DusevichV-at-umkc.edu Tue Jun 9 15:58:06 2009
8, 23 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n59Kw6Zc010639
8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 15:58:06 -0500
8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Tue, 9 Jun 2009 15:58:06 -0500
8, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: 3D software for SEM
8, 23 -- Date: Tue, 9 Jun 2009 15:58:05 -0500
8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB869-at-KC-MSX1.kc.umkc.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: 3D software for SEM
8, 23 -- Thread-Index: AcnpRPwpQ80QbyvWRl2h6jXpXlMehQ==
8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 09 Jun 2009 20:58:06.0410 (UTC) FILETIME=[FCBD92A0:01C9E944]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n59Kw6Zc010639
==============================End of - Headers==============================




From: riba-at-umd.edu
Date: Tue, 9 Jun 2009 19:31:31 -0500
Subject: [Microscopy] viaWWW: TEM JEOL 4000 FX Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both riba-at-umd.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: riba-at-umd.edu
Name: Lourdes Salamanca-Riba

Organization: University of Maryland

Title-Subject: [Filtered] TEM JEOL 4000 FX

Question: Is anyone interested in getting a JEOL 4000 FX TEM as a
whole or for parts? The microscope was operational until a year ago
when it was shut down because of lack of use. We now need the room
where the microscope is and want to know if there is any interest in
any or all of the components. We would give the microscope for free
but you would be responsible for packing it and shipping it to a new
location.

I don't know how many of these microscopes are still operational.

Thank you,

Lourdes Salamanca-Riba

Login Host: 129.2.175.79
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Jun 9 19:31:31 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5A0VUOP012751
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 19:31:30 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c654af38cc95-at-[206.69.208.22]}
9, 11 -- Date: Tue, 9 Jun 2009 19:31:29 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: riba-at-umd.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: TEM JEOL 4000 FX Available
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jpare-at-emory.edu
Date: Tue, 9 Jun 2009 19:31:57 -0500
Subject: [Microscopy] viaWWW: Coolwell chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jpare-at-emory.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jpare-at-emory.edu
Name: Jeff ParÈ

Organization: Yerkes Research Center/ Emory University

Title-Subject: [Filtered] Coolwell chiller

Question: Good afternoon,

We have an old Coolwell chiller (Model S-075W)
here and we are starting to have problems with it
as it's not keeping the scope (Zeiss EM-10C) at a
reasonable temperature. The compressor works when
we bypass the high temp indicator but it looks
like the Thermostat is not functioning properly.

Anyone in here in possession of the electrical
diagram of this machine or one similar?

Please help! :)

Thanks

Jeff

Login Host: 170.140.166.234
---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Tue Jun 9 19:31:57 2009
12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5A0VugZ013277
12, 13 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 19:31:57 -0500
12, 13 -- Mime-Version: 1.0
12, 13 -- Message-Id: {p06240801c654af6ad83d-at-[206.69.208.22]}
12, 13 -- Date: Tue, 9 Jun 2009 19:31:56 -0500
12, 13 -- To: microscopy-at-microscopy.com
12, 13 -- From: jpare-at-emory.edu (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Coolwell chiller
12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
12, 13 -- Content-Transfer-Encoding: 8bit
12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5A0VugZ013277
==============================End of - Headers==============================




From: probe-at-geotrack.com.au
Date: Tue, 9 Jun 2009 22:16:30 -0500
Subject: [Microscopy] Zeiss axiotron MCU 26 controller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
We are having a problem with our zeiss axiotron MCU 26 controller, it does
not initialise and emits an ear piercing tone on startup and goes no
further. Any information on firmware ( we were thinking possibly corrupted
EPROM) or circuit schematics to aid in repairing would be greatly
appreciated. We have had no luck with info from zeiss.
Thanks in advance
Pat

Patrick R Kelly Operations Manager
Geotrack International Pty Ltd ABN16 006 821 209
37 Melville Road, Brunswick West, Victoria 3055 Australia
Telephone: +613 93801077 Facsimile: +613 93801477
http://www.geotrack.com.au


==============================Original Headers==============================
3, 24 -- From probe-at-geotrack.com.au Tue Jun 9 22:16:30 2009
3, 24 -- Received: from geotrack.com.au (geotrack.com.au [150.101.155.223])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5A3GTOH011357
3, 24 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jun 2009 22:16:30 -0500
3, 24 -- Received: from PAT (pat.geotrack.com.au [192.168.5.18])
3, 24 -- by geotrack.com.au (Postfix) with SMTP id C3D151C00135
3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 13:16:28 +1000 (EST)
3, 24 -- Message-ID: {732ACDC7EB0344798EF290B0178FC705-at-geotrack.com.au}
3, 24 -- Reply-To: "Pat Kelly" {probe-at-geotrack.com.au}
3, 24 -- From: "Pat Kelly" {probe-at-geotrack.com.au}
3, 24 -- To: {microscopy-at-microscopy.com}
3, 24 -- Subject: Zeiss axiotron MCU 26 controller
3, 24 -- Date: Wed, 10 Jun 2009 13:16:24 +1000
3, 24 -- Organization: Geotrack International
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- format=flowed;
3, 24 -- charset="iso-8859-1";
3, 24 -- reply-type=original
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- X-Priority: 3
3, 24 -- X-MSMail-Priority: Normal
3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Wed, 10 Jun 2009 04:44:24 -0500
Subject: [Microscopy] Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gatan include 'Digital Montage' in the software available for their cameras,
that seemed to work well stitching together the TEM images [it was only used
with their camera, albeit with an offline workstation]. But it was
fantastically expensive [well the camera was and it came as a package]. See:
http://ftp.gatan.com/products/digital_imaging/products/Digital_Montage.php
Not used it since I left UCL a couple of years ago though, but it went down
well with the TEM users [the software balances intensities between images as
well]. The Montage image files were large though.

I always found Photoshop CS2/CS3 Photomerge to be pretty awful at
photo-stitching photographs and I've long abandoned it [I'll try the CS4
version next time to see if it has improved any]. In fact the software that
came with my camera* 'Olympus Master' [after you paid for the Pro upgrade]
worked far better [for camera photos] so I still stick with that. It can't
get it right every time and so a manual edit to taste is often required
[camera lens distortions and positional errors are presumably worse than
that found in 'flat' 2D TEM images]. Try things like: copy & paste badly
affected areas, and Free Transform, and Transform 'Distort' etc. to overcome
poor stitching at the overlap [plus see the link below]. Image analysis
packages like MetaMorph & AxioVision seem to go for the more conservative
Montage, where the 'overlap' edge is left as is, but I don't see much
problem ethically with very minor photo editing to get it to look nice
assuming nothing of importance is edited away or added [generally though
'looking nice' is just for the web or presentations, being aesthetics and
often of no particular scientific importance].

You can buy various similar photo-stitching panorama software, all largely
aimed at photographers though:

http://www.cs.ubc.ca/~mbrown/autostitch/autostitch.html
[seems well regarded]

http://www.ptgui.com/info/photo_stitching.html

http://www.vrtoolbox.com

http://www.easypano.com/photo-stitch-software.html

http://www.panobuilder.com/index.asp

http://www.vextrasoft.com/rasterstitch.htm

This is just a selection, and prices vary [and all can't perform miracles,
although the results can be very impressive after a minor edit or smudge
here and there]. I tried a few on demo, but my Olympus E500 SLR camera's
Olympus Pro* software did as well so I never bothered 'upgrading'. Often
it's brightness differences rather than stitching that really ruins things,
so camera lock on the same area of the view and move to the next one in
sequence] - likewise on the microscope keep exposure times as identical as
possible [I'm an optical microscope guy]. Better photo-stitching software
should attempt to correct uneven lighting.

If you have a decent digital camera, though do try panoramas as fantastic
ones are really easy to create days [although it does mean I take around
12,000 photos a year these days]. Plus the software to do it should have
come free with the camera.


Keith

E.g. Typical photo stitch help guide
http://www.phong.com/tutorials/photostitch

*e.g. Canon cameras offer similar free photo-stitch software that is highly
regarded.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: reinhard.rachel-at-biologie.uni-regensburg.de
[mailto:reinhard.rachel-at-biologie.uni-regensburg.de]
Sent: 05 June 2009 22:50
To: kjmorris-at-well.ox.ac.uk

}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Photoshop CS2's Photomerge function works well on my LM flatfield images,
} but with TEM images sometimes there are misalignments in some areas. I
} assume this is due to spherical aberration in the TEM images. Can any of
} the other software options out there detect and correct for spherical
} aberration?
}
} Ralph Common
} Michigan State University

In our lab, people now use PS CS4, with - I assume - further improved
function(s) for image stitching / merging. At least, the results are very
good. - Photomerge.
TEM: this very much depends on the magnification. It works +/- easily and
automatically (Photomerge) with images taken in medium / high mag mode
(above 3000 x). In low mag mode on our CM12, images contain obvious
distortions, preventing perfect alignments. - People who are well trained in
PS CS4 can use built-in functions for correcting these distortions, before
the images are merged. Not easy, but works satisfactorily.
I just checked PS CS4 again: "Photomerge" even includes filters ("layout")
offering some preliminary correction of distortions.
HTH - best regards - Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
7, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jun 5 16:44:17
2009
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de
(rrzmta1.rz.uni-regensburg.de [194.94.155.51])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n55LiGDG001162
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:44:16
-0500
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
7, 26 -- by localhost (Postfix) with SMTP id 3FC06ABFAD
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
+0200 (CEST)
7, 26 -- Received: from gwsmtp1.uni-regensburg.de
(gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
7, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id
37693ABFD0
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
+0200 (CEST)
7, 26 -- Received: from uni-regensburg-smtp1-MTA by
gwsmtp1.uni-regensburg.de
7, 26 -- with Novell_GroupWise; Fri, 05 Jun 2009 23:44:15 +0200
7, 26 -- Message-Id: {4A29ADC802000054000161F6-at-gwsmtp1.uni-regensburg.de}
7, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
7, 26 -- Date: Fri, 05 Jun 2009 23:44:08 +0200
7, 26 -- From: "reinhard rachel"
{reinhard.rachel-at-biologie.uni-regensburg.de}
7, 26 -- To: {rcommon-at-msu.edu}
7, 26 -- Cc: {Microscopy-at-microscopy.com}
7, 26 -- Subject: Image stitching
7, 26 -- References: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- In-Reply-To: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- Mime-Version: 1.0
7, 26 -- Content-Type: text/plain; charset=US-ASCII
7, 26 -- Content-Disposition: inline
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n55LiGDG001162
==============================End of - Headers==============================


==============================Original Headers==============================
29, 20 -- From kjmorris-at-well.ox.ac.uk Wed Jun 10 04:44:24 2009
29, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5A9iNkO010297
29, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Jun 2009 04:44:23 -0500
29, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
29, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
29, 20 -- id 1MEKM3-000728-Ec
29, 20 -- for Microscopy-at-Microscopy.Com; Wed, 10 Jun 2009 10:44:23 +0100
29, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
29, 20 -- To: {Microscopy-at-Microscopy.Com}
29, 20 -- Subject: FW: [Microscopy] Image stitching
29, 20 -- Date: Wed, 10 Jun 2009 10:44:24 +0100
29, 20 -- Message-ID: {DE2F92C5E09C4899804B1A678E319D89-at-CytoWhizz}
29, 20 -- MIME-Version: 1.0
29, 20 -- Content-Type: text/plain;
29, 20 -- charset="us-ascii"
29, 20 -- Content-Transfer-Encoding: 7bit
29, 20 -- X-Mailer: Microsoft Office Outlook 11
29, 20 -- Thread-Index: AcnmJ5S4eGjfMuLRTraVkz1lbHzamwCyPRQgAC+WBaAAAAuL4A==
29, 20 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: jose-at-svi.nl
Date: Wed, 10 Jun 2009 05:49:10 -0500
Subject: [Microscopy] Re: FW: Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I once saw an impressive demo of this freeware software, with fluorescence data:

http://www.xuvtools.org/

You may want to give it a try!

Cheers,

jose.

==============================Original Headers==============================
5, 37 -- From jose.vr-at-gmail.com Wed Jun 10 05:49:10 2009
5, 37 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
5, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AAn9LN029718
5, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 05:49:09 -0500
5, 37 -- Received: by fxm8 with SMTP id 8so751280fxm.18
5, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 03:49:09 -0700 (PDT)
5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 37 -- d=gmail.com; s=gamma;
5, 37 -- h=domainkey-signature:mime-version:sender:received:in-reply-to
5, 37 -- :references:from:date:x-google-sender-auth:message-id:subject:to
5, 37 -- :content-type:content-transfer-encoding;
5, 37 -- bh=1+b69lkTCbVK6P1kxQEZ7NGt1vIYjwYxQHNM582n+bc=;
5, 37 -- b=d0QH3K1lZJ2RS5KZc0zgNp4VRwA3917ysf2LRJvmluDIJi3lUmAml8bebt3XBaCj5v
5, 37 -- T9SHbnb5TL5Af+Wgm1WbwDK8t5qpvEMhxBMSnHFiI23EfBPd7iyvmW8CadQYoFbnIN4O
5, 37 -- scqTlWAc8/fUmwkkotuvWqSsqKuRPE84StyiE=
5, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 37 -- d=gmail.com; s=gamma;
5, 37 -- h=mime-version:sender:in-reply-to:references:from:date
5, 37 -- :x-google-sender-auth:message-id:subject:to:content-type
5, 37 -- :content-transfer-encoding;
5, 37 -- b=ON3gyzvN9S5IAL3RCoKMYZjun/fDaLYrcFxEEAX3EZmu19jrx4E1ilLx41C0LxJNDT
5, 37 -- hVuR2HC+kz82YkzDIes3F+kuNxib/O49JReWPgF1MaCBCMAAoX1c2X/VhUNPJSkQ5WkO
5, 37 -- mSLyrTvxmCjAAF6bbfiBtMLQ9W3teb4FNH5cc=
5, 37 -- MIME-Version: 1.0
5, 37 -- Sender: jose.vr-at-gmail.com
5, 37 -- Received: by 10.204.72.129 with SMTP id m1mr1179411bkj.61.1244630947769; Wed,
5, 37 -- 10 Jun 2009 03:49:07 -0700 (PDT)
5, 37 -- In-Reply-To: {200906100944.n5A9ijCe010725-at-ns.microscopy.com}
5, 37 -- References: {200906100944.n5A9ijCe010725-at-ns.microscopy.com}
5, 37 -- From: =?ISO-8859-1?Q?Jose_Vi=F1a?= {jose-at-svi.nl}
5, 37 -- Date: Wed, 10 Jun 2009 12:48:47 +0200
5, 37 -- X-Google-Sender-Auth: 607be364bb0dcd76
5, 37 -- Message-ID: {1d2213e40906100348u5eb819d0p36fb4b510c98bfea-at-mail.gmail.com}
5, 37 -- Subject: Re: [Microscopy] FW: Image stitching
5, 37 -- To: Microscopy-at-microscopy.com
5, 37 -- Content-Type: text/plain; charset=ISO-8859-1
5, 37 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: cogswell-at-nbnet.nb.ca
Date: Wed, 10 Jun 2009 08:29:24 -0500
Subject: [Microscopy] viaWWW: Image Stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cogswell-at-nbnet.nb.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cogswell-at-nbnet.nb.ca
Name: Steven Cogswell

Organization: UNB Microscopy

Title-Subject: [Filtered] Image Stitching

Question: Hi folks;

On the subject of image stitching, I'll admit I've never used it with
any EM work, but I have had tremendous success with camera panoramas
with hugin:

http://hugin.sourceforge.net/

Not only does it to the best job of things I've used, the best part
is it's Free Software. Lots of power over the stitching and final
image projection.

Some folks on flickr tag their photos with it:

http://www.flickr.com/search/?q=hugin

although not everything is hugin related.

I have used my crappy old Canon Powershot's Photostitch software for
stitching TEM images together, which was pretty succesful. If I was
to do it again I'd definitely be trying Hugin.

Best regards,
Steven Cogswell

Login Host: 131.202.42.169
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 08:29:22 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5ADTKbb021778
14, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 08:29:20 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240800c65565a18dfc-at-[206.69.208.22]}
14, 11 -- Date: Wed, 10 Jun 2009 08:29:19 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: cogswell-at-nbnet.nb.ca (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: Image Stitching
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sbarlow-at-sunstroke.sdsu.edu
Date: Wed, 10 Jun 2009 12:39:41 -0500
Subject: [Microscopy] FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Those of you who have a variable pressure microscope: Do you
still coat your samples? We are considering upgrading to a FE-VP
SEM, While the sales people and the brochures assure me that coating
will no longer be necessary, in the real world, do you sometimes
forego the low KV and/or VP and coat the sample for high resolution,
high vacuum work?

If coating is still necessary from time to time, what is the current
choice for metals? Chromium oxidizes rapidly; some have suggested
iridium; am I ready to use osmium for something besides fixation......?

Thanks in advance for any and all advice.

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive MC-4614
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

==============================Original Headers==============================
5, 19 -- From sbarlow-at-sunstroke.sdsu.edu Wed Jun 10 12:39:41 2009
5, 19 -- Received: from sciences.sdsu.edu (sciences.sdsu.edu [130.191.140.33])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AHdelT021063
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 12:39:40 -0500
5, 19 -- Received: from [146.244.225.180] ([146.244.225.180])
5, 19 -- by sciences.sdsu.edu (8.14.3/8.12.10/SCEC-042008-9-50) with ESMTP id n5AHclHp005165
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 10:38:48 -0700 (PDT)
5, 19 -- Mime-Version: 1.0
5, 19 -- Message-Id: {p06240814c6559f10e4db-at-[146.244.225.180]}
5, 19 -- Date: Wed, 10 Jun 2009 10:39:11 -0700
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- From: Steve Barlow {sbarlow-at-sunstroke.sdsu.edu}
5, 19 -- Subject: FE -variable pressure SEM and sputter coating
5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 19 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-1.6 (sciences.sdsu.edu [130.191.140.33]); Wed, 10 Jun 2009 10:38:48 -0700 (PDT)
5, 19 -- X-COS-MailScanner: Found to be clean
5, 19 -- X-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, score=-99.9,
5, 19 -- required 8, RDNS_NONE 0.10, USER_IN_WHITELIST -100.00)
5, 19 -- X-COS-MailScanner-From: sbarlow-at-sunstroke.sdsu.edu
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 10 Jun 2009 12:58:52 -0500
Subject: [Microscopy] RE: FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We coat about 90% of our specimens, reserving VP and Wet mode only for
special specimens and special occasions.. High vacuum mode usually
brings better results both for imaging and for EDS. We have biological
specimens, ceramics, metals, plastics, etc.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



}
} Those of you who have a variable pressure microscope: Do you
} still coat your samples? We are considering upgrading to a
} FE-VP SEM, While the sales people and the brochures assure
} me that coating will no longer be necessary, in the real
} world, do you sometimes forego the low KV and/or VP and coat
} the sample for high resolution, high vacuum work?
}
} If coating is still necessary from time to time, what is the
} current choice for metals? Chromium oxidizes rapidly; some
} have suggested iridium; am I ready to use osmium for
} something besides fixation......?
}
} Thanks in advance for any and all advice.
}
} Steve
} --
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} 5500 Campanile Drive MC-4614
} San Diego CA 92182-4614
} phone: (619) 594-4523
} fax: (619) 594-5676
}
} email: sbarlow-at-sunstroke.sdsu.edu
} http://www.sci.sdsu.edu/emfacility
}
} ==============================Original
} Headers==============================
} 5, 19 -- From sbarlow-at-sunstroke.sdsu.edu Wed Jun 10 12:39:41
} 2009 5, 19 -- Received: from sciences.sdsu.edu
} (sciences.sdsu.edu [130.191.140.33])
} 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n5AHdelT021063
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun
} 2009 12:39:40 -0500
} 5, 19 -- Received: from [146.244.225.180] ([146.244.225.180])
} 5, 19 -- by sciences.sdsu.edu
} (8.14.3/8.12.10/SCEC-042008-9-50) with ESMTP id n5AHclHp005165
} 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun
} 2009 10:38:48 -0700 (PDT)
} 5, 19 -- Mime-Version: 1.0
} 5, 19 -- Message-Id: {p06240814c6559f10e4db-at-[146.244.225.180]}
} 5, 19 -- Date: Wed, 10 Jun 2009 10:39:11 -0700 5, 19 -- To:
} Microscopy-at-microscopy.com 5, 19 -- From: Steve Barlow
} {sbarlow-at-sunstroke.sdsu.edu} 5, 19 -- Subject: FE -variable
} pressure SEM and sputter coating 5, 19 -- Content-Type:
} text/plain; charset="us-ascii" ; format="flowed"
} 5, 19 -- X-Greylist: Sender IP whitelisted, not delayed by
} milter-greylist-1.6 (sciences.sdsu.edu [130.191.140.33]);
} Wed, 10 Jun 2009 10:38:48 -0700 (PDT) 5, 19 --
} X-COS-MailScanner: Found to be clean 5, 19 --
} X-MailScanner-SpamCheck: not spam, SpamAssassin (not cached,
} score=-99.9,
} 5, 19 -- required 8, RDNS_NONE 0.10, USER_IN_WHITELIST -100.00)
} 5, 19 -- X-COS-MailScanner-From: sbarlow-at-sunstroke.sdsu.edu
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Wed Jun 10 12:58:52 2009
7, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AHwpWV004754
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 12:58:51 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Wed, 10 Jun 2009 12:58:50 -0500
7, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] FE -variable pressure SEM and sputter coating
7, 25 -- Date: Wed, 10 Jun 2009 12:58:50 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB86A-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200906101740.n5AHeOks022006-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] FE -variable pressure SEM and sputter coating
7, 25 -- Thread-Index: Acnp8om/+07QQa4kSjmbG159WAdPEAAARnlg
7, 25 -- References: {200906101740.n5AHeOks022006-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {sbarlow-at-sunstroke.sdsu.edu} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 10 Jun 2009 17:58:50.0818 (UTC) FILETIME=[1C523A20:01C9E9F5]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5AHwpWV004754
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Wed, 10 Jun 2009 15:29:18 -0500
Subject: [Microscopy] FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Dr. Barlow,

You will almost always get a better image with coated materials. Insulating samples can still charge- and in new and often unusual ways. Depending on the tech/detection system the charge is masked by an opposing ion cloud. This works well for homogeneous surfaces, but not so much for complex materials/surfaces where charge distribution is varied across the surface. Metal coating mitigates this though a light and transparent carbon coat works well too.

In a conventional ESEM the higher atomic number metal coatings lower the interaction volumes thereby decreasing the effective beam size allowing better resolving power/higher mags. Carbon does not help in this respect much. Heat is also dissipated better allowing lower accelerating voltages with good secondary yield. Low vac needs higher acc. voltages for the BSE based detectors and to some extent the gaseous secondary. You mention a FE-SEM which could be expected to have great emission at very low accelerating voltages so in your case the above is not as much of an issue as with my LaB6 based machine. Our other FE-ESEM generates top notch images of uncoated materials and produces top notch quant. analyses uncoated. If yours is anything like it you could expect great end results either way- but again, interaction volume is the primary limitation on ultimate resolution and all things being equal, the higher z coating will resolve better in most instances.

What it will all boil down to however is a financial justification in a busy lab. Almost every biologic material we have examined took longer to image uncoated. Quant analysis is also easier on coated samples. The cost of the coater is more than made up for with the increased productivity. I can look at years of data generated on my conventional Lab6 ESEM and for like samples the researchers looking at the coated materials spent much less time here than those uncoated and often got better images. I wish I could give you a realistic number to use for cost recovery, but the FE-ESEM is in another department and they don't track usage statistics in the same way that I do. And remember it only takes 15min to coat a sample.

In short, I would get the coater.

Hope this helps,

Sincerely,

Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891

-----Original Message-----
X-from: sbarlow-at-sunstroke.sdsu.edu [mailto:sbarlow-at-sunstroke.sdsu.edu]
Sent: Wednesday, June 10, 2009 1:41 PM
To: Whittaker, Scott

Those of you who have a variable pressure microscope: Do you
still coat your samples? We are considering upgrading to a FE-VP
SEM, While the sales people and the brochures assure me that coating
will no longer be necessary, in the real world, do you sometimes
forego the low KV and/or VP and coat the sample for high resolution,
high vacuum work?

If coating is still necessary from time to time, what is the current
choice for metals? Chromium oxidizes rapidly; some have suggested
iridium; am I ready to use osmium for something besides fixation......?

Thanks in advance for any and all advice.

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive MC-4614
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

==============================Original Headers==============================
5, 19 -- From sbarlow-at-sunstroke.sdsu.edu Wed Jun 10 12:39:41 2009
5, 19 -- Received: from sciences.sdsu.edu (sciences.sdsu.edu [130.191.140.33])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AHdelT021063
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 12:39:40 -0500
5, 19 -- Received: from [146.244.225.180] ([146.244.225.180])
5, 19 -- by sciences.sdsu.edu (8.14.3/8.12.10/SCEC-042008-9-50) with ESMTP id n5AHclHp005165
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 10:38:48 -0700 (PDT)
5, 19 -- Mime-Version: 1.0
5, 19 -- Message-Id: {p06240814c6559f10e4db-at-[146.244.225.180]}
5, 19 -- Date: Wed, 10 Jun 2009 10:39:11 -0700
5, 19 -- To: Microscopy-at-microscopy.com
5, 19 -- From: Steve Barlow {sbarlow-at-sunstroke.sdsu.edu}
5, 19 -- Subject: FE -variable pressure SEM and sputter coating
5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 19 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-1.6 (sciences.sdsu.edu [130.191.140.33]); Wed, 10 Jun 2009 10:38:48 -0700 (PDT)
5, 19 -- X-COS-MailScanner: Found to be clean
5, 19 -- X-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, score=-99.9,
5, 19 -- required 8, RDNS_NONE 0.10, USER_IN_WHITELIST -100.00)
5, 19 -- X-COS-MailScanner-From: sbarlow-at-sunstroke.sdsu.edu
==============================End of - Headers==============================


==============================Original Headers==============================
19, 29 -- From WHITTAKS-at-si.edu Wed Jun 10 15:29:17 2009
19, 29 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177])
19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AKTHdR026715
19, 29 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 15:29:17 -0500
19, 29 -- Received: from si-msesmtpo-01.US.SINET.SI.EDU (SI-MSESMTP-N1.us.sinet.si.edu [160.111.49.75])
19, 29 -- by si-mailout03.si.edu (Postfix) with ESMTP id F126D6781;
19, 29 -- Wed, 10 Jun 2009 16:29:16 -0400 (EDT)
19, 29 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by si-msesmtpo-01.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959);
19, 29 -- Wed, 10 Jun 2009 16:29:16 -0400
19, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 29 -- Content-class: urn:content-classes:message
19, 29 -- MIME-Version: 1.0
19, 29 -- Content-Type: text/plain;
19, 29 -- charset="iso-8859-1"
19, 29 -- Subject: RE: [Microscopy] FE -variable pressure SEM and sputter coating
19, 29 -- Date: Wed, 10 Jun 2009 16:29:15 -0400
19, 29 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA303E5062B-at-SI-ECL02.US.SINET.SI.EDU}
19, 29 -- In-Reply-To: {200906101741.n5AHfGdX023488-at-ns.microscopy.com}
19, 29 -- X-MS-Has-Attach:
19, 29 -- X-MS-TNEF-Correlator:
19, 29 -- Thread-Topic: [Microscopy] FE -variable pressure SEM and sputter coating
19, 29 -- Thread-Index: Acnp8qmL54tviMuoTsS4YO2NSJcyCAAD75GA
19, 29 -- References: {200906101741.n5AHfGdX023488-at-ns.microscopy.com}
19, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
19, 29 -- To: {sbarlow-at-sunstroke.sdsu.edu}
19, 29 -- Cc: {microscopy-at-microscopy.com}
19, 29 -- X-OriginalArrivalTime: 10 Jun 2009 20:29:16.0654 (UTC) FILETIME=[202380E0:01C9EA0A]
19, 29 -- Content-Transfer-Encoding: 8bit
19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5AKTHdR026715
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Wed, 10 Jun 2009 16:03:26 -0500
Subject: [Microscopy] Balzers CPD 020

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I've been getting rid of old equipment and found a gem(?) in the otherwise unwanted pile of stuff. I can't even give these items away since they are VA tagged, it will be excessed and then probably thrown away. Durst enlarger (with lenses), photographic paper (polycontrast and F series), polycontrast filters, old Reichert-Jung cryo unit, etc.

It's a Balzers CPD 020. Of course it's just sitting in a box with out any owner's manual. Does anyone have one they can copy and e-mail to me?

It this CPD reliable? Let me know your opinions.


Dessicating the natural way (over time),

Paula :-)


Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF)

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.





==============================Original Headers==============================
22, 21 -- From vapatpxs-at-yahoo.com Wed Jun 10 16:03:25 2009
22, 21 -- Received: from web46104.mail.sp1.yahoo.com (web46104.mail.sp1.yahoo.com [68.180.199.121])
22, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5AL3PdJ009784
22, 21 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Jun 2009 16:03:25 -0500
22, 21 -- Received: (qmail 77348 invoked by uid 60001); 10 Jun 2009 21:03:25 -0000
22, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1244667804; bh=CifwnXygmaPepCIVQXUqdqvh8x69hYS+xkbtGM/ImQI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=A1JOUgIjFwB7KH5LXSSyM4lfGMNEaV4t+yoKtB9NPsLjBM0CjyeqtPb/B9Dk5TIMUm+uDeTIGKVQq1y3QfvSPcPBr/KgA8i8Hx92NZzTcxXEEiMKY2cwPwmImI7siuXR4yIGW8/LOAk614ZbS6s/MCCYyy+9wnREqByUqLZ62F4=
22, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
22, 21 -- s=s1024; d=yahoo.com;
22, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type;
22, 21 -- b=wnM7yVfK/uCsVZ05GczdAkQ+B1RKOBEn+nCaM8jqs++N8vi+Zsb12iY/eGO5xKBMe8oobUeOHiKTBqnv3aXXw7JhN2PaLgwFG1RbZakqtWpTXUQGAMfRpoeYu6HsbCoz28x2GyzFPQ7wGpnlo002k9lUrhIM5y1DXjetjpP+pkE=;
22, 21 -- Message-ID: {996561.76719.qm-at-web46104.mail.sp1.yahoo.com}
22, 21 -- X-YMail-OSG: UIm9SJkVM1lWKk.i7x6kFnLRpXLucmWgP65Y.Op9UHve.6GeJWsaJqWNb0giYm1Zhw282B2L4OWh84IuVf42lwOo7vR.qrEJ64ss0CS55zh2k7y8ODlcxgGZYCSUmFj00D6uLDZLOLB18pbQnHOCVMrqwTC3DOJnlk30zN9Q6oEqAgwaDrn.CX4sp4xSAumdXKmBcH0DlIT0TTKDxD0r27khdMIqo1XgiFaOCR56TmV0OpL.9x9OlJUJBa9dX03TJMSV9YFM15qKWg3pJIL_63zLdkrULI9K1Wusfg--
22, 21 -- Received: from [132.239.85.200] by web46104.mail.sp1.yahoo.com via HTTP; Wed, 10 Jun 2009 14:03:24 PDT
22, 21 -- X-Mailer: YahooMailClassic/5.4.12 YahooMailWebService/0.7.289.15
22, 21 -- Date: Wed, 10 Jun 2009 14:03:24 -0700 (PDT)
22, 21 -- From: vapatpxs-at-yahoo.com
22, 21 -- Reply-To: vapatpxs-at-yahoo.com
22, 21 -- Subject: Balzers CPD 020
22, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com}
22, 21 -- MIME-Version: 1.0
22, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: helsayed-at-ucalgary.ca
Date: Wed, 10 Jun 2009 17:57:54 -0500
Subject: [Microscopy] viaWWW: Au adhesion on Ti problem....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both helsayed-at-ucalgary.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: helsayed-at-ucalgary.ca
Name: Hany

Organization: University of Calgary

Title-Subject: [Filtered] Au adhesion on Ti problem....

Question: Hi All,

I have a question regarding Au thin film adhesion on Ti. We sputter a
thin film of Ti (5 min of sputtering at 50 mA gives a Ti film of
about 15 nm) on glass slides and follow this with sputtering a Au
film (15 min of sputtering at 50 mA gives about 150 nm). We find that
Au film does not stick on Ti film and that when the Au target is
replaced by a Pt target, a Pt film adheres well to the Ti layer
underneath. We use a Denton DV-502 A sputtering machine and the
sputtering is done at about 8 m Torr Ar.

Any suggestion of why Au does not stick on Ti film?

Thanks a lot in advance

Hany

Login Host: 136.159.250.213
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 17:57:54 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AMvrJ4027927
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 17:57:54 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c655eae1c98a-at-[206.69.208.22]}
10, 11 -- Date: Wed, 10 Jun 2009 17:57:53 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: helsayed-at-ucalgary.ca (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Au adhesion on Ti problem....
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: klr-at-mts.net
Date: Wed, 10 Jun 2009 17:59:59 -0500
Subject: [Microscopy] viaWWW: Checking eyepiece and objective magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both klr-at-mts.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: klr-at-mts.net
Name: K Ringland

Title-Subject: [Filtered] Checking eyepiece and objective magnification

Question: I recently purchased a stero-dissecting microscope
secondhand, however I am having trouble believing that the
magnifications stamped on the eyepieces and objectives are true. ie
1mm by 1mm grid square does not appear to be 10mm by 10mm when I use
a 10x eyepiece and with the 1x objective, the same is true when I use
a 10x eyepiece with a 3x objective, the square does not appear 30x
bigger. Is there a way that I can check or determine the
magnification of the objectives/eyepieces? Is there an equation out
there that might help me out. Thanks.

Login Host: 205.200.7.18
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 17:59:58 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AMxv8T031647
5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 17:59:58 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c655eb58e568-at-[206.69.208.22]}
5, 11 -- Date: Wed, 10 Jun 2009 17:59:56 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: klr-at-mts.net (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Checking eyepiece and objective magnification
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hlowers-at-usgs.gov
Date: Wed, 10 Jun 2009 18:00:27 -0500
Subject: [Microscopy] viaWWW: Automated polishers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hlowers-at-usgs.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hlowers-at-usgs.gov
Name: Heather Lowers

Organization: U.S. Geological Survey

Title-Subject: [Filtered] Automated polishers

Question: I'm looking for recommendations for an automated polishing
machine that can control the depth/thickness that is removed. It
needs to accomadate petrographic thin sections and round mounts. The
goal is to do serial sectioning and analyses to creat 3D
compositional images and to have an automated polishing system.

Thanks
Heather

Login Host: 136.177.22.60
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 18:00:27 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AN0QOV000701
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 18:00:27 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240804c655eb74ec00-at-[206.69.208.22]}
7, 11 -- Date: Wed, 10 Jun 2009 18:00:26 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: hlowers-at-usgs.gov (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Automated polishers
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Wed, 10 Jun 2009 19:18:39 -0500
Subject: [Microscopy] Re: viaWWW: Automated polishers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Heather,

You might look at the RoboMet. I know that it has been successfully
used for 3D reconstruction in one of the labs here. I believe that some
people at OSU contributed to its development, but I have no personal
interest in the company.

http://www.ues.com/images/Robo-Met.3D%20Insert.pdf

Cheers,
Henk
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both hlowers-at-usgs.gov as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: hlowers-at-usgs.gov
} Name: Heather Lowers
}
} Organization: U.S. Geological Survey
}
} Title-Subject: [Filtered] Automated polishers
}
} Question: I'm looking for recommendations for an automated polishing
} machine that can control the depth/thickness that is removed. It
} needs to accomadate petrographic thin sections and round mounts. The
} goal is to do serial sectioning and analyses to creat 3D
} compositional images and to have an automated polishing system.
}
} Thanks
} Heather
}
} Login Host: 136.177.22.60
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 18:00:27 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AN0QOV000701
} 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 18:00:27 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240804c655eb74ec00-at-[206.69.208.22]}
} 7, 11 -- Date: Wed, 10 Jun 2009 18:00:26 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: hlowers-at-usgs.gov (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Automated polishers
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
6, 26 -- From colijn.1-at-osu.edu Wed Jun 10 19:18:38 2009
6, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B0IcnH008342
6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 19:18:38 -0500
6, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
6, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
6, 26 -- id {01NA049S20DC94VOGJ-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
6, 26 -- Wed, 10 Jun 2009 20:18:37 -0400 (EDT)
6, 26 -- Received: from [192.168.1.129]
6, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
6, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
6, 26 -- with ESMTPA id {01NA049RCTCW94R4PG-at-ecr6.ohio-state.edu} ; Wed,
6, 26 -- 10 Jun 2009 20:18:37 -0400 (EDT)
6, 26 -- Date: Wed, 10 Jun 2009 20:18:36 -0400
6, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
6, 26 -- Subject: Re: [Microscopy] viaWWW: Automated polishers
6, 26 -- In-reply-to: {200906102302.n5AN24ab006304-at-ns.microscopy.com}
6, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
6, 26 -- To: hlowers-at-usgs.gov, microscopy-at-microscopy.com
6, 26 -- Message-id: {4A304D5C.1060900-at-osu.edu}
6, 26 -- MIME-version: 1.0
6, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
6, 26 -- Content-transfer-encoding: 7bit
6, 26 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
6, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
6, 26 -- References: {200906102302.n5AN24ab006304-at-ns.microscopy.com}
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Wed, 10 Jun 2009 19:21:16 -0500
Subject: [Microscopy] Re: viaWWW: Automated polishers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Heather,

You might look at the RoboMet. I know that it has been successfully
used for 3D reconstruction in one of the labs here. I believe that some
people at OSU contributed to its development, but I have no personal
interest in the company.

http://www.ues.com/images/Robo-Met.3D%20Insert.pdf

Cheers,
Henk
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
}
---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
replying
} please copy both hlowers-at-usgs.gov as well as the MIcroscopy
Listserver
}
---------------------------------------------------------------------------
}
} Email: hlowers-at-usgs.gov
} Name: Heather Lowers
}
} Organization: U.S. Geological Survey
}
} Title-Subject: [Filtered] Automated polishers
}
} Question: I'm looking for recommendations for an automated polishing
} machine that can control the depth/thickness that is removed. It
} needs to accomadate petrographic thin sections and round mounts. The
} goal is to do serial sectioning and analyses to creat 3D
} compositional images and to have an automated polishing system.
}
} Thanks
} Heather
}
} Login Host: 136.177.22.60
}
---------------------------------------------------------------------------
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
6, 24 -- From colijn.1-at-osu.edu Wed Jun 10 19:21:16 2009
6, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B0LGGO011683
6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 19:21:16 -0500
6, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
6, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
6, 24 -- id {01NA04D1QB1S94U5TL-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
6, 24 -- Wed, 10 Jun 2009 20:21:15 -0400 (EDT)
6, 24 -- Received: from [192.168.1.129]
6, 24 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
6, 24 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
6, 24 -- with ESMTPA id {01NA04D1622M94TUNT-at-ecr6.ohio-state.edu} ; Wed,
6, 24 -- 10 Jun 2009 20:21:15 -0400 (EDT)
6, 24 -- Date: Wed, 10 Jun 2009 20:21:15 -0400
6, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
6, 24 -- Subject: Re: [Microscopy] viaWWW: Automated polishers
6, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
6, 24 -- To: hlowers-at-usgs.gov, microscopy-at-microscopy.com
6, 24 -- Message-id: {4A304DFB.8070601-at-osu.edu}
6, 24 -- MIME-version: 1.0
6, 24 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
6, 24 -- Content-transfer-encoding: 7bit
6, 24 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
6, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Wed, 10 Jun 2009 19:25:23 -0500
Subject: [Microscopy] Re: FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve,

We do a lot of low vacuum imaging with our FEI NOVA nanoSEM and FEI Quanta
3D FEG. I like to coat whenever possible as I do believe that I get better
images as a result. I often do not do so if we want EDX on samples that are
basically low atomic # or have trace elements we want to detect. I do not
want the coating to mask anything. However, coating for a lot of samples
does not disturb the EDX results...you just end up with a peak that has to
be explained.

We also do ESEM work but since that is almost always hydrated samples,
coating is not possible.

We coat with either AuPd or Pt. I always coat with Pt if I think we will
need higher magnifications. You can see the Au grain but not the Pt grain
at magnifications well over 50,000x (30x26 display size). The new coater we
got (with Pt target) when we got our first FESEM has proved it's worth
beyond a doubt. We could not function without it. It is heavily used for
high vacuum since we do a lot of very high mag imaging...nano everything
...with samples often nonconductive or deposited on non-conductive
materials. Using it for low vac when possible is a no brainer...better
images with lots less hassle.

And, in my opinion, high vacuum imaging always gives sharper images (better
resolution, than low vac so use it when possible with coating as needed.
However, the very concentrated beams of a FESEM results in major charging
issues in some samples such as particulate or fibrous ones. That's where
low vac really shines.

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy






On 6/10/09 1:41 PM, "Steven Barlow" {sbarlow-at-sunstroke.sdsu.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Those of you who have a variable pressure microscope: Do you
} still coat your samples? We are considering upgrading to a FE-VP
} SEM, While the sales people and the brochures assure me that coating
} will no longer be necessary, in the real world, do you sometimes
} forego the low KV and/or VP and coat the sample for high resolution,
} high vacuum work?
}
} If coating is still necessary from time to time, what is the current
} choice for metals? Chromium oxidizes rapidly; some have suggested
} iridium; am I ready to use osmium for something besides fixation......?
}
} Thanks in advance for any and all advice.
}
} Steve


==============================Original Headers==============================
16, 31 -- From dsherman-at-purdue.edu Wed Jun 10 19:25:22 2009
16, 31 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129])
16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B0PMsl023537
16, 31 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 19:25:22 -0500
16, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
16, 31 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n5B0PLcR016401
16, 31 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 20:25:22 -0400
16, 31 -- Received: from 1061exfe03a.purdue.lcl (1061exfe03a.itap.purdue.edu [128.210.1.10])
16, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n5B0PLld010079
16, 31 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 20:25:21 -0400
16, 31 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe03a.purdue.lcl with Microsoft SMTPSVC(6.0.3790.3959);
16, 31 -- Wed, 10 Jun 2009 20:25:21 -0400
16, 31 -- Received: from 24.12.228.135 ([24.12.228.135]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ;
16, 31 -- Thu, 11 Jun 2009 00:25:19 +0000
16, 31 -- User-Agent: Microsoft-Entourage/12.17.0.090302
16, 31 -- Date: Wed, 10 Jun 2009 20:25:17 -0400
16, 31 -- Subject: Re: [Microscopy] FE -variable pressure SEM and sputter coating
16, 31 -- From: Debby Sherman {dsherman-at-purdue.edu}
16, 31 -- To: Steven Barlow {sbarlow-at-sunstroke.sdsu.edu} ,
16, 31 -- "message to: MSA list" {microscopy-at-microscopy.com}
16, 31 -- Message-ID: {C655C72D.29729%dsherman-at-purdue.edu}
16, 31 -- Thread-Topic: [Microscopy] FE -variable pressure SEM and sputter coating
16, 31 -- Thread-Index: AcnqKxhcsCxyx7qeNEO1F3KIwcgjwA==
16, 31 -- In-Reply-To: {200906101741.n5AHflcV024395-at-ns.microscopy.com}
16, 31 -- Mime-version: 1.0
16, 31 -- Content-type: text/plain;
16, 31 -- charset="US-ASCII"
16, 31 -- Content-transfer-encoding: 7bit
16, 31 -- X-OriginalArrivalTime: 11 Jun 2009 00:25:21.0748 (UTC) FILETIME=[1B315140:01C9EA2B]
16, 31 -- X-PMX-Version: 5.4.0.320885
16, 31 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 10 Jun 2009 21:04:28 -0500
Subject: [Microscopy] Re: FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IMO, VP means Very Poor imaging. It is a great idea
but not all that great in reality. An ESEM may be
different but this discussion is relative to VP.

For an FESEM with VP, the column is about 1/3
taller than a non-VP column. This makes the gun/tip and
column alignment much more difficult and fleeting over
time. Current systems like Zeiss Supra and Ultra will
do imaging at low KV (500V-1KV) and may not exhibit charging.
But this is not a patent rule. Thus, high vacuum with
coating is IMO always the best route to high quality
images. It takes little time to coat. But the specimen
is irreversibly altered as a result. If this is an issue,
the best that you can get from VP is a solution of compromise.

Specimens are an issue, relative to fixation,
drying and morphology. So it is a conundrum from start
to finish.

If I want high quality images, which I do, I coat and
use HV. I agree that this is not optimal for bio
specimens that are not fixed and CPD. This is a
tough area for SEM imaging.

gary g.




At 10:41 AM 6/10/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
13, 20 -- From gary-at-gaugler.com Wed Jun 10 21:04:28 2009
13, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5B24SqL020829
13, 20 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 21:04:28 -0500
13, 20 -- Message-Id: {200906110204.n5B24SqL020829-at-ns.microscopy.com}
13, 20 -- Received: (qmail 13913 invoked from network); 10 Jun 2009 18:56:10 -0700
13, 20 -- Received: by simscan 1.1.0 ppid: 13910, pid: 13911, t: 0.1059s
13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
13, 20 -- by smtp2 with SMTP; 10 Jun 2009 18:56:10 -0700
13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
13, 20 -- Date: Wed, 10 Jun 2009 19:04:29 -0700
13, 20 -- To: sbarlow-at-sunstroke.sdsu.edu
13, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
13, 20 -- Subject: Re: [Microscopy] FE -variable pressure SEM and sputter coating
13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
13, 20 -- In-Reply-To: {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
13, 20 -- References: {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
13, 20 -- Mime-Version: 1.0
13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Wed, 10 Jun 2009 21:22:40 -0500
Subject: [Microscopy] FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Forgot to copy to the list. As noted below, if you're imaging type
specimens, you can't coat with anything, so VP is the only way to go.

Dear Steve,

The best thing about not coating is the structural detail you can get in
BSE mode. We (i.e. our SEM expert, not me...) look at plant material, and
we can get cell outlines and other internal structural detail very easily in
uncoated tissue imaged uncoated in VP mode - for rapid image analysis
measuring cell sizes, for example. This is very difficult any other way
because in standard SEM mode with coated material, while the human brain can
easily discriminate cell outlines from changes in grey levels, the software
has a very hard time.

The other key application is imaging type specimens of plants and insects
which cannot under any circumstances be coated.

It's been a real boost for us. Sure, we do CPD or cryo when necessary, and
mainly coat with gold - most of our work is at relatively low mag - up to
about 10k.

cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 11/06/09 12:09 PM, "gary-at-gaugler.com" {gary-at-gaugler.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} IMO, VP means Very Poor imaging. It is a great idea
} but not all that great in reality. An ESEM may be
} different but this discussion is relative to VP.
}
} For an FESEM with VP, the column is about 1/3
} taller than a non-VP column. This makes the gun/tip and
} column alignment much more difficult and fleeting over
} time. Current systems like Zeiss Supra and Ultra will
} do imaging at low KV (500V-1KV) and may not exhibit charging.
} But this is not a patent rule. Thus, high vacuum with
} coating is IMO always the best route to high quality
} images. It takes little time to coat. But the specimen
} is irreversibly altered as a result. If this is an issue,
} the best that you can get from VP is a solution of compromise.
}
} Specimens are an issue, relative to fixation,
} drying and morphology. So it is a conundrum from start
} to finish.
}
} If I want high quality images, which I do, I coat and
} use HV. I agree that this is not optimal for bio
} specimens that are not fixed and CPD. This is a
} tough area for SEM imaging.
}
} gary g.
}
}
}
}
} At 10:41 AM 6/10/2009, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Those of you who have a variable pressure microscope: Do you
} } still coat your samples? We are considering upgrading to a FE-VP
} } SEM, While the sales people and the brochures assure me that coating
} } will no longer be necessary, in the real world, do you sometimes
} } forego the low KV and/or VP and coat the sample for high resolution,
} } high vacuum work?
} }
} } If coating is still necessary from time to time, what is the current
} } choice for metals? Chromium oxidizes rapidly; some have suggested
} } iridium; am I ready to use osmium for something besides fixation......?
} }
} } Thanks in advance for any and all advice.
} }
} } Steve
} } --
} } Dr. Steven Barlow
} } EM Facility/Biology Dept.
} } San Diego State University
} } 5500 Campanile Drive MC-4614
} } San Diego CA 92182-4614
} } phone: (619) 594-4523
} } fax: (619) 594-5676
} }
} } email: sbarlow-at-sunstroke.sdsu.edu
} } http://www.sci.sdsu.edu/emfacility
} }
} } ==============================Original Headers==============================
} } 5, 19 -- From sbarlow-at-sunstroke.sdsu.edu Wed Jun 10 12:39:41 2009
} } 5, 19 -- Received: from sciences.sdsu.edu (sciences.sdsu.edu
} } [130.191.140.33])
} } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n5AHdelT021063
} } 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009
} } 12:39:40 -0500
} } 5, 19 -- Received: from [146.244.225.180] ([146.244.225.180])
} } 5, 19 -- by sciences.sdsu.edu
} } (8.14.3/8.12.10/SCEC-042008-9-50) with ESMTP id n5AHclHp005165
} } 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jun 2009
} } 10:38:48 -0700 (PDT)
} } 5, 19 -- Mime-Version: 1.0
} } 5, 19 -- Message-Id: {p06240814c6559f10e4db-at-[146.244.225.180]}
} } 5, 19 -- Date: Wed, 10 Jun 2009 10:39:11 -0700
} } 5, 19 -- To: Microscopy-at-microscopy.com
} } 5, 19 -- From: Steve Barlow {sbarlow-at-sunstroke.sdsu.edu}
} } 5, 19 -- Subject: FE -variable pressure SEM and sputter coating
} } 5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } 5, 19 -- X-Greylist: Sender IP whitelisted, not delayed by
} } milter-greylist-1.6 (sciences.sdsu.edu [130.191.140.33]); Wed, 10
} } Jun 2009 10:38:48 -0700 (PDT)
} } 5, 19 -- X-COS-MailScanner: Found to be clean
} } 5, 19 -- X-MailScanner-SpamCheck: not spam, SpamAssassin (not
} } cached, score=-99.9,
} } 5, 19 -- required 8, RDNS_NONE 0.10, USER_IN_WHITELIST -100.00)
} } 5, 19 -- X-COS-MailScanner-From: sbarlow-at-sunstroke.sdsu.edu
} } ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 13, 20 -- From gary-at-gaugler.com Wed Jun 10 21:04:28 2009
} 13, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net
} [66.60.130.145])
} 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} n5B24SqL020829
} 13, 20 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 21:04:28 -0500
} 13, 20 -- Message-Id: {200906110204.n5B24SqL020829-at-ns.microscopy.com}
} 13, 20 -- Received: (qmail 13913 invoked from network); 10 Jun 2009 18:56:10
} -0700
} 13, 20 -- Received: by simscan 1.1.0 ppid: 13910, pid: 13911, t: 0.1059s
} 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
} 13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
} 13, 20 -- by smtp2 with SMTP; 10 Jun 2009 18:56:10 -0700
} 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 13, 20 -- Date: Wed, 10 Jun 2009 19:04:29 -0700
} 13, 20 -- To: sbarlow-at-sunstroke.sdsu.edu
} 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
} 13, 20 -- Subject: Re: [Microscopy] FE -variable pressure SEM and sputter
} coating
} 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
} 13, 20 -- In-Reply-To: {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
} 13, 20 -- References: {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
} 13, 20 -- Mime-Version: 1.0
} 13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} ==============================End of - Headers==============================



==============================Original Headers==============================
13, 44 -- From prvs=406aba5a1=Rosemary.White-at-csiro.au Wed Jun 10 21:22:39 2009
13, 44 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43])
13, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B2McwT002726
13, 44 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 21:22:38 -0500
13, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
13, 44 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
13, 44 -- s=email; t=1244686959; x=1276222959;
13, 44 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
13, 44 -- mime-version:content-transfer-encoding:content-id:
13, 44 -- content-description:resent-date:resent-from:resent-sender:
13, 44 -- resent-to:resent-cc:resent-message-id:in-reply-to:
13, 44 -- references:list-id:list-help:list-unsubscribe:
13, 44 -- list-subscribe:list-post:list-owner:list-archive;
13, 44 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
13, 44 -- |Subject:=20Re:=20[Microscopy]=20Re:=20FE=20-variable=20p
13, 44 -- ressure=20SEM=20and=20sputter=20coating|Date:=20Thu,=2011
13, 44 -- =20Jun=202009=2012:22:34=20+1000|Message-ID:=20 {C656A78A.
13, 44 -- 65DB%rosemary.white-at-csiro.au} |To:=20 {microscopy-at-microscop
13, 44 -- y.com} |MIME-Version:=201.0|Content-Transfer-Encoding:=207
13, 44 -- bit|In-Reply-To:=20 {200906110209.n5B29wcF029764-at-ns.micros
13, 44 -- copy.com} ;
13, 44 -- bh=+DbT6OBh+2EZ4HUaOD29wE62xC1TSMImd8Kw7r/uq5g=;
13, 44 -- b=oDb5JPkNilegdYBzEeoSRfK+r465IkGRyJetSdm2/cRnDGF3SQrTjlfV
13, 44 -- u0lAVufSZ6pRlyfHxcnSrAYtiOsf743vKuWFG3gqq8AJEYxc2BYeJWSrw
13, 44 -- /5onjfh7V1jdWdz;
13, 44 -- X-IronPort-AV: E=Sophos;i="4.42,199,1243778400";
13, 44 -- d="scan'208";a="28576242"
13, 44 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126])
13, 44 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 11 Jun 2009 12:22:36 +1000
13, 44 -- Received: from [152.83.193.49] (152.83.193.49) by EXNSW-HTCA01.nexus.csiro.au
13, 44 -- (130.155.117.126) with Microsoft SMTP Server id 8.1.375.2; Thu, 11 Jun 2009
13, 44 -- 12:22:32 +1000
13, 44 -- User-Agent: Microsoft-Entourage/12.10.0.080409
13, 44 -- Date: Thu, 11 Jun 2009 12:22:34 +1000
13, 44 -- Subject: Re: [Microscopy] Re: FE -variable pressure SEM and sputter coating
13, 44 -- From: Rosemary White {rosemary.white-at-csiro.au}
13, 44 -- To: {microscopy-at-microscopy.com}
13, 44 -- Message-ID: {C656A78A.65DB%rosemary.white-at-csiro.au}
13, 44 -- Thread-Topic: [Microscopy] Re: FE -variable pressure SEM and sputter coating
13, 44 -- Thread-Index: AcnqObqmryN/Itp8QwuSO/1K+h+lVgAAcAWf
13, 44 -- In-Reply-To: {200906110209.n5B29wcF029764-at-ns.microscopy.com}
13, 44 -- MIME-Version: 1.0
13, 44 -- Content-Type: text/plain; charset="US-ASCII"
13, 44 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 10 Jun 2009 21:28:13 -0500
Subject: [Microscopy] Re: FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How are you going to get morphology in BSE mode?

This is news.

gary g.


At 07:23 PM 6/10/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Wed Jun 10 21:28:13 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5B2SCvn015394
10, 20 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 21:28:13 -0500
10, 20 -- Message-Id: {200906110228.n5B2SCvn015394-at-ns.microscopy.com}
10, 20 -- Received: (qmail 23441 invoked from network); 10 Jun 2009 19:19:55 -0700
10, 20 -- Received: by simscan 1.1.0 ppid: 23437, pid: 23438, t: 0.2271s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 10 Jun 2009 19:19:55 -0700
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Wed, 10 Jun 2009 19:28:14 -0700
10, 20 -- To: rosemary.white-at-csiro.au
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] FE -variable pressure SEM and sputter coating
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200906110223.n5B2Nmfw005068-at-ns.microscopy.com}
10, 20 -- References: {200906110223.n5B2Nmfw005068-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: eikonika-at-otenet.gr
Date: Thu, 11 Jun 2009 01:00:04 -0500
Subject: [Microscopy] Re: FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Although I have a VP SEM I always dry and coat the specimens (talking about
biological material). Never dared to try the low pressure mode; I am afraid
that if the specimens are wet liquid material can explode and spoil the
microscope and then image will be poor even in the high pressure mode.
Can anybody from the list comment if my fears are justified?
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477



----- Original Message -----
X-from: {gary-at-gaugler.com}
To: {eikonika-at-otenet.gr}
Sent: Thursday, June 11, 2009 5:32 AM


==============================Original Headers==============================
9, 23 -- From eikonika-at-otenet.gr Thu Jun 11 01:00:01 2009
9, 23 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [83.235.67.30])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B5xv4S003669
9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 00:59:59 -0500
9, 23 -- Received: from axeron (athedsl-253337.home.otenet.gr [85.73.37.55])
9, 23 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id n5B5xsXp018052
9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 08:59:55 +0300
9, 23 -- Message-ID: {002401c9ea59$e3d50ff0$2101a8c0-at-axeron}
9, 23 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
9, 23 -- To: {microscopy-at-microscopy.com}
9, 23 -- References: {200906110232.n5B2WLG6025885-at-ns.microscopy.com}
9, 23 -- Subject: Re: FE -variable pressure SEM and sputter coating -my fears
9, 23 -- Date: Thu, 11 Jun 2009 09:00:15 +0300
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain;
9, 23 -- format=flowed;
9, 23 -- charset="iso-8859-7";
9, 23 -- reply-type=original
9, 23 -- Content-Transfer-Encoding: 7bit
9, 23 -- X-Priority: 3
9, 23 -- X-MSMail-Priority: Normal
9, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
9, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




From: g.h.ten.brink-at-rug.nl
Date: Thu, 11 Jun 2009 02:51:21 -0500
Subject: [Microscopy] FE -variable pressure SEM and sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nice to see so many comments about VP imaging.

A couple of practical things. Like Debby and others pointed out high vacuum
with coating gives the best results resolution wise.

We have all kinds of SEM's, XL30FEG, XL30sFEG, and one XL30FEG ESEM and I
use them all. Imaging in wet mode with water vapor goes until 20.000x mag my
best so far. With argon or nitrogen it's even more difficult to get some
contrast and thus good resolution. With the XL30sFEG 200.000 or more on
daily routine basis.

But in the ESEM you do see the true nature of things. Beside I can even
image samples at elevated temperatures (100-700 degrees C in situ and under
tensile conditions) I can't do that in any other mode.

I saw one comment about surface morphology and BSE and someone doubting it.
Well I can do it also just play with the detector quadrants (A+B or A-B
etc.) it gives nice images. Never too old to learn Gary :-) How ? the
quadrants or at an angle in respect to the origin of the BSE electrons
similar like your own eyes. It can also be that the guy mentioned BSE and
should have written GSE. But his statement was valid.

And one good thought for Yorgos Nikas, I never had anything exploding in my
ESEM nor planning to.

With kind regards,

Gert ten Brink
Dept. Applied Physics

Zernike Institute for Advanced Materials
University of Groningen
Nijenborgh 4
9747 AG Groningen
The Netherlands
phone: +31(0)503634889; fax:+31(0)503634881

e-mail: g.h.ten.brink-at-rug.nl;
http://materials-science.phys.rug.nl
http://www.m2i.nl




==============================Original Headers==============================
13, 23 -- From g.h.ten.brink-at-rug.nl Thu Jun 11 02:51:20 2009
13, 23 -- Received: from smtp2h.service.rug.nl (smtp2h.service.rug.nl [129.125.60.2])
13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5B7pJ0c022612
13, 23 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 02:51:20 -0500
13, 23 -- Received: from rugghbrink (bigip2.id.rug.nl [129.125.68.242])
13, 23 -- by smtp2h.service.rug.nl (8.14.3/8.14.3) with ESMTP id n5B7pJFW031359
13, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 09:51:19 +0200
13, 23 -- From: "Gert ten Brink" {g.h.ten.brink-at-rug.nl}
13, 23 -- To: {Microscopy-at-microscopy.com}
13, 23 -- Subject: FE -variable pressure SEM and sputter coating
13, 23 -- Date: Thu, 11 Jun 2009 09:51:14 +0200
13, 23 -- Message-ID: {000f01c9ea69$64f87540$2ee95fc0$-at-h.ten.brink-at-rug.nl}
13, 23 -- MIME-Version: 1.0
13, 23 -- Content-Type: text/plain;
13, 23 -- charset="us-ascii"
13, 23 -- Content-Transfer-Encoding: 7bit
13, 23 -- X-Mailer: Microsoft Office Outlook 12.0
13, 23 -- Thread-Index: AcnqaWMD6/0MmnXXQyqoHkxVleLW3w==
13, 23 -- Content-Language: nl
13, 23 -- x-cr-hashedpuzzle: AMSx AReO BFku EA9c EC60 EJSj EX17 Ew+I FJSB FU3p FfYl GMxd HdoC HrEf I1Ef K9Sw;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{4C3A41AD-14A8-4037-9579-EB700F6482AE};ZwAuAGgALgB0AGUAbgAuAGIAcgBpAG4AawBAAHIAdQBnAC4AbgBsAA==;Thu, 11 Jun 2009 07:51:11 GMT;RgBFACAALQB2AGEAcgBpAGEAYgBsAGUAIABwAHIAZQBzAHMAdQByAGUAIABTAEUATQAgAGEAbgBkACAAcwBwAHUAdAB0AGUAcgAgAGMAbwBhAHQAaQBuAGcA
13, 23 -- x-cr-puzzleid: {4C3A41AD-14A8-4037-9579-EB700F6482AE}
13, 23 -- X-Virus-Scanned: clamav-milter 0.95.1 at smtp2h
13, 23 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: kbarwick-at-ocsd.org
Date: Thu, 11 Jun 2009 08:47:22 -0500
Subject: [Microscopy] viaWWW: Ocular Micrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kbarwick-at-ocsd.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kbarwick-at-ocsd.org
Name: Kelvin Barwick

Organization: Orange County Sanitation District

Title-Subject: [Filtered] Ocular Micrometer

Question: Pardon me for honing in but I have a similar problem as Mr.
Ringland. My purchased Wild M5 came with an ocular micrometer but it
is divided into a twelfths. The 12 main marks are subdivided into
tenths. I have tried to line it up with metric and English rulers at
each of the magnification steps. It does not seem to be either a
system.

Kelvin


Login Host: 63.110.101.130
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 08:47:22 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BDlLXE029809
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 08:47:22 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240808c656bb57a614-at-[206.69.208.22]}
8, 11 -- Date: Thu, 11 Jun 2009 08:47:19 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: kbarwick-at-ocsd.org (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Ocular Micrometer
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dlowry-at-asu.edu
Date: Thu, 11 Jun 2009 08:47:46 -0500
Subject: [Microscopy] viaWWW: Lowicryl low-temperature embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] Lowicryl low-temperature embedding

Question: we are interested in attempting the use of a Lowicryl resin
for infiltrating and embedding samples at low sub-zero temperature.
How does one go about handling and processing samples at these
temperatures--does this normally require access to special
refrigerators and instruments, or are there strategies using more
common equipment and perhaps dry ice that will work? Any advice is
greatly appreciated.

Login Host: 129.219.58.218
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 08:47:46 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BDljMe029951
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 08:47:45 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240809c656bb70ac08-at-[206.69.208.22]}
6, 11 -- Date: Thu, 11 Jun 2009 08:47:45 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: dlowry-at-asu.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Lowicryl low-temperature embedding
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 11 Jun 2009 08:56:56 -0500
Subject: [Microscopy] FE -variable pressure SEM and sputter coating -my fears

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To observe wet specimens you need not VP but Environmental SEM, capable
to keep water vapor pressure at 5-6 torr and maintain specimen
temperature at about 5 degrees Celsius, to be close to a dew point to
minimize evaporation. Putting wet specimen to VP scope will result in
fast water evaporation, up to boiling (if specimen big enough), plus
gases dissolved in water could escape violently. So yes, "explosion" is
possible.

When working with wet specimens in wet mode it is helpful to put a few
droplets of water on the specimen stage to get water vapors in chamber
as fast as possible during pumping down (otherwise there is a
possibility of drying out specimen during pumping with rehydrating it
when equilibrium is reached). Escaping (from water droplets) gases can
send splashes in all directions, and it makes necessary to withdraw EDS
detector as far as possible to prevent window rupture.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} Although I have a VP SEM I always dry and coat the specimens
} (talking about biological material). Never dared to try the
} low pressure mode; I am afraid that if the specimens are wet
} liquid material can explode and spoil the microscope and then
} image will be poor even in the high pressure mode.
} Can anybody from the list comment if my fears are justified?
} yorgos
}
} Dr Yorgos Nikas
} Athens Innovative Microscopy
} Skra 36 Voula 16673 GREECE
}
} eikonika-at-otenet.gr
} yorgosnikas-at-hotmail.com
} Tel/fax +30 210 8957677
} Mobile +30 6945 107477
}
}
}
} ----- Original Message -----
} X-from: {gary-at-gaugler.com}
} To: {eikonika-at-otenet.gr}
} Sent: Thursday, June 11, 2009 5:32 AM
} Subject: [Microscopy] Re: FE -variable pressure SEM and
} sputter coating
}
}
} }
} }
} }
} }
} --------------------------------------------------------------
} --------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} }
} } How are you going to get morphology in BSE mode?
} }
} } This is news.
} }
} } gary g.
} }
} }
} } At 07:23 PM 6/10/2009, you wrote:
} }
} }
} }
} } } ------------------------------------------------------------
} ----------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ------------------------------------------------------------
} ----------------
} } }
} } } Forgot to copy to the list. As noted below, if you're imaging type
} } } specimens, you can't coat with anything, so VP is the only
} way to go.
} } }
} } } Dear Steve,
} } }
} } } The best thing about not coating is the structural detail
} you can get in
} } } BSE mode. We (i.e. our SEM expert, not me...) look at
} plant material, and
} } } we can get cell outlines and other internal structural
} detail very easily
} } } in
} } } uncoated tissue imaged uncoated in VP mode - for rapid
} image analysis
} } } measuring cell sizes, for example. This is very difficult
} any other way
} } } because in standard SEM mode with coated material, while
} the human brain
} } } can
} } } easily discriminate cell outlines from changes in grey levels, the
} } } software
} } } has a very hard time.
} } }
} } } The other key application is imaging type specimens of
} plants and insects
} } } which cannot under any circumstances be coated.
} } }
} } } It's been a real boost for us. Sure, we do CPD or cryo
} when necessary,
} } } and
} } } mainly coat with gold - most of our work is at relatively
} low mag - up to
} } } about 10k.
} } }
} } } cheers,
} } } Rosemary
} } }
} } } Rosemary White
} } } CSIRO Plant Industry
} } } GPO Box 1600
} } } Canberra, ACT 2601
} } } Australia
} } }
} } } ph 61 2 6246 5475
} } } fx 61 2 6246 5334
} } }
} } }
} } } On 11/06/09 12:09 PM, "gary-at-gaugler.com" {gary-at-gaugler.com} wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} --------------------------------------------------------------
} --------------
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of
} } } } America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} --------------------------------------------------------------
} --------------
} } } }
} } } } IMO, VP means Very Poor imaging. It is a great idea
} } } } but not all that great in reality. An ESEM may be
} } } } different but this discussion is relative to VP.
} } } }
} } } } For an FESEM with VP, the column is about 1/3
} } } } taller than a non-VP column. This makes the gun/tip and
} } } } column alignment much more difficult and fleeting over
} } } } time. Current systems like Zeiss Supra and Ultra will
} } } } do imaging at low KV (500V-1KV) and may not exhibit charging.
} } } } But this is not a patent rule. Thus, high vacuum with
} } } } coating is IMO always the best route to high quality
} } } } images. It takes little time to coat. But the specimen
} } } } is irreversibly altered as a result. If this is an issue,
} } } } the best that you can get from VP is a solution of compromise.
} } } }
} } } } Specimens are an issue, relative to fixation,
} } } } drying and morphology. So it is a conundrum from start
} } } } to finish.
} } } }
} } } } If I want high quality images, which I do, I coat and
} } } } use HV. I agree that this is not optimal for bio
} } } } specimens that are not fixed and CPD. This is a
} } } } tough area for SEM imaging.
} } } }
} } } } gary g.
} } } }
} } } }
} } } }
} } } }
} } } } At 10:41 AM 6/10/2009, you wrote:
} } } }
} } } }
} } } }
} } } } }
} } }
} --------------------------------------------------------------
} --------------
} } } } } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of
} } } } } America
} } } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } }
} --------------------------------------------------------------
} --------------
} } } } }
} } } } } Those of you who have a variable pressure microscope: Do you
} } } } } still coat your samples? We are considering upgrading
} to a FE-VP
} } } } } SEM, While the sales people and the brochures assure
} me that coating
} } } } } will no longer be necessary, in the real world, do you sometimes
} } } } } forego the low KV and/or VP and coat the sample for
} high resolution,
} } } } } high vacuum work?
} } } } }
} } } } } If coating is still necessary from time to time, what
} is the current
} } } } } choice for metals? Chromium oxidizes rapidly; some
} have suggested
} } } } } iridium; am I ready to use osmium for something besides
} } } } } fixation......?
} } } } }
} } } } } Thanks in advance for any and all advice.
} } } } }
} } } } } Steve
} } } } } --
} } } } } Dr. Steven Barlow
} } } } } EM Facility/Biology Dept.
} } } } } San Diego State University
} } } } } 5500 Campanile Drive MC-4614
} } } } } San Diego CA 92182-4614
} } } } } phone: (619) 594-4523
} } } } } fax: (619) 594-5676
} } } } }
} } } } } email: sbarlow-at-sunstroke.sdsu.edu
} } } } } http://www.sci.sdsu.edu/emfacility
} } } } }
} } } } } ==============================Original
} } } Headers==============================
} } } } } 5, 19 -- From sbarlow-at-sunstroke.sdsu.edu Wed Jun 10
} 12:39:41 2009
} } } } } 5, 19 -- Received: from sciences.sdsu.edu (sciences.sdsu.edu
} } } } } [130.191.140.33])
} } } } } 5, 19 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with
} } } } } ESMTP id n5AHdelT021063
} } } } } 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed,
} 10 Jun 2009
} } } } } 12:39:40 -0500
} } } } } 5, 19 -- Received: from [146.244.225.180] ([146.244.225.180])
} } } } } 5, 19 -- by sciences.sdsu.edu
} } } } } (8.14.3/8.12.10/SCEC-042008-9-50) with ESMTP id n5AHclHp005165
} } } } } 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed,
} 10 Jun 2009
} } } } } 10:38:48 -0700 (PDT)
} } } } } 5, 19 -- Mime-Version: 1.0
} } } } } 5, 19 -- Message-Id: {p06240814c6559f10e4db-at-[146.244.225.180]}
} } } } } 5, 19 -- Date: Wed, 10 Jun 2009 10:39:11 -0700
} } } } } 5, 19 -- To: Microscopy-at-microscopy.com
} } } } } 5, 19 -- From: Steve Barlow {sbarlow-at-sunstroke.sdsu.edu}
} } } } } 5, 19 -- Subject: FE -variable pressure SEM and sputter coating
} } } } } 5, 19 -- Content-Type: text/plain; charset="us-ascii" ;
} } } } } format="flowed"
} } } } } 5, 19 -- X-Greylist: Sender IP whitelisted, not delayed by
} } } } } milter-greylist-1.6 (sciences.sdsu.edu
} [130.191.140.33]); Wed, 10
} } } } } Jun 2009 10:38:48 -0700 (PDT)
} } } } } 5, 19 -- X-COS-MailScanner: Found to be clean
} } } } } 5, 19 -- X-MailScanner-SpamCheck: not spam, SpamAssassin (not
} } } } } cached, score=-99.9,
} } } } } 5, 19 -- required 8, RDNS_NONE 0.10,
} USER_IN_WHITELIST -100.00)
} } } } } 5, 19 -- X-COS-MailScanner-From: sbarlow-at-sunstroke.sdsu.edu
} } } } } ==============================End of -
} } } Headers==============================
} } } }
} } } }
} } } } ==============================Original
} } } Headers==============================
} } } } 13, 20 -- From gary-at-gaugler.com Wed Jun 10 21:04:28 2009
} } } } 13, 20 -- Received: from smtp2.mc.surewest.net
} (qsmtp.mc.surewest.net
} } } } [66.60.130.145])
} } } } 13, 20 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with SMTP id
} } } } n5B24SqL020829
} } } } 13, 20 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009
} } } } 21:04:28 -0500
} } } } 13, 20 -- Message-Id:
} {200906110204.n5B24SqL020829-at-ns.microscopy.com}
} } } } 13, 20 -- Received: (qmail 13913 invoked from network); 10 Jun
} } } 2009 18:56:10
} } } } -0700
} } } } 13, 20 -- Received: by simscan 1.1.0 ppid: 13910, pid: 13911, t:
} } } } 0.1059s
} } } } 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
} } } } 13, 20 -- Received: from unknown (HELO thor.gaugler.com)
} } } } (66.60.171.211)
} } } } 13, 20 -- by smtp2 with SMTP; 10 Jun 2009 18:56:10 -0700
} } } } 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} } } } 13, 20 -- Date: Wed, 10 Jun 2009 19:04:29 -0700
} } } } 13, 20 -- To: sbarlow-at-sunstroke.sdsu.edu
} } } } 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
} } } } 13, 20 -- Subject: Re: [Microscopy] FE -variable
} pressure SEM and
} } } } sputter
} } } } coating
} } } } 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
} } } } 13, 20 -- In-Reply-To:
} {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
} } } } 13, 20 -- References:
} {200906101741.n5AHfKSF023625-at-ns.microscopy.com}
} } } } 13, 20 -- Mime-Version: 1.0
} } } } 13, 20 -- Content-Type: text/plain; charset="us-ascii";
} format=flowed
} } } } ==============================End of -
} } } Headers==============================
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 13, 44 -- From prvs=406aba5a1=Rosemary.White-at-csiro.au Wed
} Jun 10 21:22:39
} } } 2009
} } } 13, 44 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au
} } } [150.229.7.43])
} } } 13, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } } ESMTP id n5B2McwT002726
} } } 13, 44 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009
} } } 21:22:38 -0500
} } } 13, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
} } } 13, 44 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
} } } 13, 44 -- s=email; t=1244686959; x=1276222959;
} } } 13, 44 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
} } } 13, 44 -- mime-version:content-transfer-encoding:content-id:
} } } 13, 44 --
} content-description:resent-date:resent-from:resent-sender:
} } } 13, 44 -- resent-to:resent-cc:resent-message-id:in-reply-to:
} } } 13, 44 -- references:list-id:list-help:list-unsubscribe:
} } } 13, 44 -- list-subscribe:list-post:list-owner:list-archive;
} } } 13, 44 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
} } } 13, 44 --
} |Subject:=20Re:=20[Microscopy]=20Re:=20FE=20-variable=20p
} } } 13, 44 --
} ressure=20SEM=20and=20sputter=20coating|Date:=20Thu,=2011
} } } 13, 44 --
} =20Jun=202009=2012:22:34=20+1000|Message-ID:=20 {C656A78A.
} } } 13, 44 --
} 65DB%rosemary.white-at-csiro.au} |To:=20 {microscopy-at-microscop
} } } 13, 44 --
} y.com} |MIME-Version:=201.0|Content-Transfer-Encoding:=207
} } } 13, 44 --
} bit|In-Reply-To:=20 {200906110209.n5B29wcF029764-at-ns.micros
} } } 13, 44 -- copy.com} ;
} } } 13, 44 -- bh=+DbT6OBh+2EZ4HUaOD29wE62xC1TSMImd8Kw7r/uq5g=;
} } } 13, 44 --
} b=oDb5JPkNilegdYBzEeoSRfK+r465IkGRyJetSdm2/cRnDGF3SQrTjlfV
} } } 13, 44 --
} u0lAVufSZ6pRlyfHxcnSrAYtiOsf743vKuWFG3gqq8AJEYxc2BYeJWSrw
} } } 13, 44 -- /5onjfh7V1jdWdz;
} } } 13, 44 -- X-IronPort-AV: E=Sophos;i="4.42,199,1243778400";
} } } 13, 44 -- d="scan'208";a="28576242"
} } } 13, 44 -- Received: from exnsw-htca01.nexus.csiro.au
} ([130.155.117.126])
} } } 13, 44 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 11
} } } Jun 2009 12:22:36 +1000
} } } 13, 44 -- Received: from [152.83.193.49] (152.83.193.49) by
} } } EXNSW-HTCA01.nexus.csiro.au
} } } 13, 44 -- (130.155.117.126) with Microsoft SMTP Server id
} } } 8.1.375.2; Thu, 11 Jun 2009
} } } 13, 44 -- 12:22:32 +1000
} } } 13, 44 -- User-Agent: Microsoft-Entourage/12.10.0.080409
} } } 13, 44 -- Date: Thu, 11 Jun 2009 12:22:34 +1000
} } } 13, 44 -- Subject: Re: [Microscopy] Re: FE -variable pressure SEM
} } } and sputter coating
} } } 13, 44 -- From: Rosemary White {rosemary.white-at-csiro.au}
} } } 13, 44 -- To: {microscopy-at-microscopy.com}
} } } 13, 44 -- Message-ID: {C656A78A.65DB%rosemary.white-at-csiro.au}
} } } 13, 44 -- Thread-Topic: [Microscopy] Re: FE -variable pressure SEM
} } } and sputter coating
} } } 13, 44 -- Thread-Index: AcnqObqmryN/Itp8QwuSO/1K+h+lVgAAcAWf
} } } 13, 44 -- In-Reply-To:
} {200906110209.n5B29wcF029764-at-ns.microscopy.com}
} } } 13, 44 -- MIME-Version: 1.0
} } } 13, 44 -- Content-Type: text/plain; charset="US-ASCII"
} } } 13, 44 -- Content-Transfer-Encoding: 7bit
} } } ==============================End of -
} } } Headers==============================
} }
} }
} } ==============================Original
} } Headers==============================
} } 10, 20 -- From gary-at-gaugler.com Wed Jun 10 21:28:13 2009
} } 10, 20 -- Received: from smtp2.mc.surewest.net
} (qsmtp.mc.surewest.net
} } [66.60.130.145])
} } 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id
} } n5B2SCvn015394
} } 10, 20 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009
} 21:28:13 -0500
} } 10, 20 -- Message-Id:
} {200906110228.n5B2SCvn015394-at-ns.microscopy.com}
} } 10, 20 -- Received: (qmail 23441 invoked from network); 10 Jun 2009
} } 19:19:55 -0700
} } 10, 20 -- Received: by simscan 1.1.0 ppid: 23437, pid:
} 23438, t: 0.2271s
} } 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
} } 10, 20 -- Received: from unknown (HELO thor.gaugler.com)
} (66.60.171.211)
} } 10, 20 -- by smtp2 with SMTP; 10 Jun 2009 19:19:55 -0700
} } 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} } 10, 20 -- Date: Wed, 10 Jun 2009 19:28:14 -0700
} } 10, 20 -- To: rosemary.white-at-csiro.au
} } 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
} } 10, 20 -- Subject: Re: [Microscopy] FE -variable pressure
} SEM and sputter
} } coating
} } 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
} } 10, 20 -- In-Reply-To:
} {200906110223.n5B2Nmfw005068-at-ns.microscopy.com}
} } 10, 20 -- References:
} {200906110223.n5B2Nmfw005068-at-ns.microscopy.com}
} } 10, 20 -- Mime-Version: 1.0
} } 10, 20 -- Content-Type: text/plain; charset="us-ascii";
} format=flowed
} } ==============================End of -
} } Headers==============================
} }
}
}
} ==============================Original
} Headers==============================
} 9, 23 -- From eikonika-at-otenet.gr Thu Jun 11 01:00:01 2009
} 9, 23 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr
} [83.235.67.30])
} 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n5B5xv4S003669
} 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun
} 2009 00:59:59 -0500
} 9, 23 -- Received: from axeron (athedsl-253337.home.otenet.gr
} [85.73.37.55])
} 9, 23 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3)
} with SMTP id n5B5xsXp018052
} 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun
} 2009 08:59:55 +0300
} 9, 23 -- Message-ID: {002401c9ea59$e3d50ff0$2101a8c0-at-axeron}
} 9, 23 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
} 9, 23 -- To: {microscopy-at-microscopy.com}
} 9, 23 -- References: {200906110232.n5B2WLG6025885-at-ns.microscopy.com}
} 9, 23 -- Subject: Re: FE -variable pressure SEM and sputter
} coating -my fears
} 9, 23 -- Date: Thu, 11 Jun 2009 09:00:15 +0300
} 9, 23 -- MIME-Version: 1.0
} 9, 23 -- Content-Type: text/plain;
} 9, 23 -- format=flowed;
} 9, 23 -- charset="iso-8859-7";
} 9, 23 -- reply-type=original
} 9, 23 -- Content-Transfer-Encoding: 7bit
} 9, 23 -- X-Priority: 3
} 9, 23 -- X-MSMail-Priority: Normal
} 9, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
} 9, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
} ==============================End of -
} Headers==============================
}
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Thu Jun 11 08:56:56 2009
7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BDutpq009568
7, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 08:56:55 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Thu, 11 Jun 2009 08:56:55 -0500
7, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] Re: FE -variable pressure SEM and sputter coating -my fears
7, 25 -- Date: Thu, 11 Jun 2009 08:56:54 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB86C-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200906110602.n5B62IHw004561-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] Re: FE -variable pressure SEM and sputter coating -my fears
7, 25 -- Thread-Index: AcnqWjGG0FkkBLAUQtGwsuPhY8Z4KgAP8dtw
7, 25 -- References: {200906110602.n5B62IHw004561-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {eikonika-at-otenet.gr} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 11 Jun 2009 13:56:55.0224 (UTC) FILETIME=[7AC41B80:01C9EA9C]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5BDutpq009568
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 11 Jun 2009 10:34:19 -0500
Subject: [Microscopy] Re: viaWWW: Ocular Micrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ocular scales are neither english or metric system. The scale must be
calibrated for each magnification you wish to use it at with a stage
micrometer or precision ruler (dependent on the magnification and accuracy
wanted).

Basically, you line up a ocular scale line with scale of a calibrated
ruler, find a second OSL that matches a line on your ruler, count the
number of OS units and the distance they occupy.

Now you know that 26 (for example) OSU occupy 1.1 mm so each OSU is 1.1/26
or 0.042 mm or 42 microns. Do the sample with the english system.

Repeat at a new magnification until you've done all the desired mags..

I think that's what you're asking...




kbarwick-at-ocsd.org

06/11/2009 10:07 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
kbarwick-at-ocsd.org [Microscopy] viaWWW: Ocular
Micrometer













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both kbarwick-at-ocsd.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kbarwick-at-ocsd.org
Name: Kelvin Barwick

Organization: Orange County Sanitation District

Title-Subject: [Filtered] Ocular Micrometer

Question: Pardon me for honing in but I have a similar problem as Mr.
Ringland. My purchased Wild M5 came with an ocular micrometer but it
is divided into a twelfths. The 12 main marks are subdivided into
tenths. I have tried to line it up with metric and English rulers at
each of the magnification steps. It does not seem to be either a
system.

Kelvin


Login Host: 63.110.101.130
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 08:47:22 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5BDlLXE029809
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009
08:47:22 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240808c656bb57a614-at-[206.69.208.22]}
8, 11 -- Date: Thu, 11 Jun 2009 08:47:19 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: kbarwick-at-ocsd.org (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Ocular Micrometer
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
29, 22 -- From frank_karl-at-lincolnelectric.com Thu Jun 11 10:34:18 2009
29, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
29, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BFYGgR011546
29, 22 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 10:34:17 -0500
29, 22 -- In-Reply-To: {200906111407.n5BE7qD6002257-at-ns.microscopy.com}
29, 22 -- Subject: Re: [Microscopy] viaWWW: Ocular Micrometer
29, 22 -- To: kbarwick-at-ocsd.org, Microscopy-at-microscopy.com
29, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
29, 22 -- Message-ID: {OF385BAC33.4364201B-ON852575D2.005458EC-852575D2.005580F5-at-lincolnelectric.com}
29, 22 -- Date: Thu, 11 Jun 2009 11:33:56 -0400
29, 22 -- From: Frank_Karl-at-lincolnelectric.com
29, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
29, 22 -- 07, 2008) at 06/11/2009 11:33:57 AM,
29, 22 -- CD-MIME complete at 06/11/2009 11:33:57 AM,
29, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
29, 22 -- 07, 2008) at 06/11/2009 11:33:57 AM,
29, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
29, 22 -- 07, 2008) at 06/11/2009 11:33:57 AM,
29, 22 -- Serialize complete at 06/11/2009 11:33:57 AM
29, 22 -- MIME-Version: 1.0
29, 22 -- Content-Type: text/plain;
29, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Thu, 11 Jun 2009 11:43:26 -0500
Subject: [Microscopy] Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had a go with Photoshop CS4's PhotoMerge*, and yes it is doing a far
better job than the older Photoshop version I last used [probably CS2] - and
it's now producing better output than my Olympus Master Pro in manual mode
[although the Olympus cameras have a hardware panorama mode of sequential
capture that works far more reliably]. The SLR barrel lens distortion
effects remained, but the stitching was pretty perfect. I'll give it five,
although it was slow at the stitching [presumably because it's thinking
hard]. I expect dedicated commercial stitching software has likewise
improved over the last couple of years - I'll check them out as demos, but I
can live with CS4's output.

I've never had a problem with slightly varied light levels on a scientific
montage of say a completely scanned section using Zeiss Axiovision and a
motorized stage - it kinda emphasizes that it is a montage and easily
identifies each specific image within the entire raster scan. For a scene of
Tenby harbor in the real world though it does look appalling.

Keith

*I have to say I think Photoshop CS4 is the best update since Photoshop CS
arrived.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: Ralph Common [mailto:rcommon-at-msu.edu]
Sent: 10 June 2009 15:22
To: kjmorris-at-well.ox.ac.uk

Gatan include 'Digital Montage' in the software available for their cameras,
that seemed to work well stitching together the TEM images [it was only used
with their camera, albeit with an offline workstation]. But it was
fantastically expensive [well the camera was and it came as a package]. See:
http://ftp.gatan.com/products/digital_imaging/products/Digital_Montage.php
Not used it since I left UCL a couple of years ago though, but it went down
well with the TEM users [the software balances intensities between images as
well]. The Montage image files were large though.

I always found Photoshop CS2/CS3 Photomerge to be pretty awful at
photo-stitching photographs and I've long abandoned it [I'll try the CS4
version next time to see if it has improved any]. In fact the software that
came with my camera* 'Olympus Master' [after you paid for the Pro upgrade]
worked far better [for camera photos] so I still stick with that. It can't
get it right every time and so a manual edit to taste is often required
[camera lens distortions and positional errors are presumably worse than
that found in 'flat' 2D TEM images]. Try things like: copy & paste badly
affected areas, and Free Transform, and Transform 'Distort' etc. to overcome
poor stitching at the overlap [plus see the link below]. Image analysis
packages like MetaMorph & AxioVision seem to go for the more conservative
Montage, where the 'overlap' edge is left as is, but I don't see much
problem ethically with very minor photo editing to get it to look nice
assuming nothing of importance is edited away or added [generally though
'looking nice' is just for the web or presentations, being aesthetics and
often of no particular scientific importance].

You can buy various similar photo-stitching panorama software, all largely
aimed at photographers though:

http://www.cs.ubc.ca/~mbrown/autostitch/autostitch.html
[seems well regarded]

http://www.ptgui.com/info/photo_stitching.html

http://www.vrtoolbox.com

http://www.easypano.com/photo-stitch-software.html

http://www.panobuilder.com/index.asp

http://www.vextrasoft.com/rasterstitch.htm

This is just a selection, and prices vary [and all can't perform miracles,
although the results can be very impressive after a minor edit or smudge
here and there]. I tried a few on demo, but my Olympus E500 SLR camera's
Olympus Pro* software did as well so I never bothered 'upgrading'. Often
it's brightness differences rather than stitching that really ruins things,
so camera lock on the same area of the view and move to the next one in
sequence] - likewise on the microscope keep exposure times as identical as
possible [I'm an optical microscope guy]. Better photo-stitching software
should attempt to correct uneven lighting.

If you have a decent digital camera, though do try panoramas as fantastic
ones are really easy to create days [although it does mean I take around
12,000 photos a year these days]. Plus the software to do it should have
come free with the camera.


Keith

E.g. Typical photo stitch help guide
http://www.phong.com/tutorials/photostitch

*e.g. Canon cameras offer similar free photo-stitch software that is highly
regarded.

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: reinhard.rachel-at-biologie.uni-regensburg.de
[mailto:reinhard.rachel-at-biologie.uni-regensburg.de]
Sent: 05 June 2009 22:50
To: kjmorris-at-well.ox.ac.uk

}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Photoshop CS2's Photomerge function works well on my LM flatfield images,
} but with TEM images sometimes there are misalignments in some areas. I
} assume this is due to spherical aberration in the TEM images. Can any of
} the other software options out there detect and correct for spherical
} aberration?
}
} Ralph Common
} Michigan State University

In our lab, people now use PS CS4, with - I assume - further improved
function(s) for image stitching / merging. At least, the results are very
good. - Photomerge.
TEM: this very much depends on the magnification. It works +/- easily and
automatically (Photomerge) with images taken in medium / high mag mode
(above 3000 x). In low mag mode on our CM12, images contain obvious
distortions, preventing perfect alignments. - People who are well trained in
PS CS4 can use built-in functions for correcting these distortions, before
the images are merged. Not easy, but works satisfactorily.
I just checked PS CS4 again: "Photomerge" even includes filters ("layout")
offering some preliminary correction of distortions.
HTH - best regards - Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
7, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jun 5 16:44:17
2009
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de
(rrzmta1.rz.uni-regensburg.de [194.94.155.51])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n55LiGDG001162
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:44:16
-0500
7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
7, 26 -- by localhost (Postfix) with SMTP id 3FC06ABFAD
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
+0200 (CEST)
7, 26 -- Received: from gwsmtp1.uni-regensburg.de
(gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
7, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id
37693ABFD0
7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
+0200 (CEST)
7, 26 -- Received: from uni-regensburg-smtp1-MTA by
gwsmtp1.uni-regensburg.de
7, 26 -- with Novell_GroupWise; Fri, 05 Jun 2009 23:44:15 +0200
7, 26 -- Message-Id: {4A29ADC802000054000161F6-at-gwsmtp1.uni-regensburg.de}
7, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
7, 26 -- Date: Fri, 05 Jun 2009 23:44:08 +0200
7, 26 -- From: "reinhard rachel"
{reinhard.rachel-at-biologie.uni-regensburg.de}
7, 26 -- To: {rcommon-at-msu.edu}
7, 26 -- Cc: {Microscopy-at-microscopy.com}
7, 26 -- Subject: Image stitching
7, 26 -- References: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- In-Reply-To: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
7, 26 -- Mime-Version: 1.0
7, 26 -- Content-Type: text/plain; charset=US-ASCII
7, 26 -- Content-Disposition: inline
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n55LiGDG001162
==============================End of - Headers==============================


==============================Original Headers==============================
29, 20 -- From kjmorris-at-well.ox.ac.uk Wed Jun 10 04:44:24 2009
29, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk
[129.67.44.2])
29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5A9iNkO010297
29, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Jun 2009 04:44:23
-0500
29, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178]
helo=CytoWhizz)
29, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
29, 20 -- id 1MEKM3-000728-Ec
29, 20 -- for Microscopy-at-Microscopy.Com; Wed, 10 Jun 2009 10:44:23
+0100
29, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
29, 20 -- To: {Microscopy-at-Microscopy.Com}
29, 20 -- Subject: FW: [Microscopy] Image stitching
29, 20 -- Date: Wed, 10 Jun 2009 10:44:24 +0100
29, 20 -- Message-ID: {DE2F92C5E09C4899804B1A678E319D89-at-CytoWhizz}
29, 20 -- MIME-Version: 1.0
29, 20 -- Content-Type: text/plain;
29, 20 -- charset="us-ascii"
29, 20 -- Content-Transfer-Encoding: 7bit
29, 20 -- X-Mailer: Microsoft Office Outlook 11
29, 20 -- Thread-Index: AcnmJ5S4eGjfMuLRTraVkz1lbHzamwCyPRQgAC+WBaAAAAuL4A==
29, 20 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================



==============================Original Headers==============================
48, 22 -- From kjmorris-at-well.ox.ac.uk Thu Jun 11 11:43:26 2009
48, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
48, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BGhPHg027550
48, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 11 Jun 2009 11:43:26 -0500
48, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz)
48, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
48, 22 -- id 1MEnN7-0004lf-9w
48, 22 -- for Microscopy-at-Microscopy.Com; Thu, 11 Jun 2009 17:43:25 +0100
48, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
48, 22 -- To: {Microscopy-at-Microscopy.Com}
48, 22 -- References: {200906100948.n5A9mLde016355-at-ns.microscopy.com} {00c401c9e9d6$ddd9ee50$7d7a0923-at-msu.edu}
48, 22 -- In-Reply-To: {00c401c9e9d6$ddd9ee50$7d7a0923-at-msu.edu}
48, 22 -- Subject: RE: [Microscopy] FW: Image stitching
48, 22 -- Date: Thu, 11 Jun 2009 17:43:25 +0100
48, 22 -- Message-ID: {001501c9eab3$bd585c60$38091520$-at-ox.ac.uk}
48, 22 -- MIME-Version: 1.0
48, 22 -- Content-Type: text/plain;
48, 22 -- charset="US-ASCII"
48, 22 -- Content-Transfer-Encoding: 7bit
48, 22 -- X-Mailer: Microsoft Office Outlook 12.0
48, 22 -- thread-index: Acnp1v35dvQPTfH/TX6btz39f8PDOAAn2ZEw
48, 22 -- Content-Language: en-gb
==============================End of - Headers==============================




From: Elliott-at-arizona.edu
Date: Thu, 11 Jun 2009 12:08:23 -0500
Subject: [Microscopy] FW: Image stitching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Photoshop has the ability to correct for barrel (or pincushion) lens
distortion.
I think it is under Filter -} Distort -} Lens Correction. The
process can be automated with a Photoshop 'Action'.

If you are using both a standard camera (SLR or P&S), there is a
great Photoshop plugin called LensFix that is very capable at fixing
geometric distortion.
http://www.kekus.com/software/plugin.html

No financial interest in either company, just a happy user.

David


On Jun 11, 2009, at 9:48 AM, kjmorris-at-well.ox.ac.uk wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} I have had a go with Photoshop CS4's PhotoMerge*, and yes it is
} doing a far
} better job than the older Photoshop version I last used [probably
} CS2] - and
} it's now producing better output than my Olympus Master Pro in
} manual mode
} [although the Olympus cameras have a hardware panorama mode of
} sequential
} capture that works far more reliably]. The SLR barrel lens distortion
} effects remained, but the stitching was pretty perfect. I'll give
} it five,
} although it was slow at the stitching [presumably because it's
} thinking
} hard]. I expect dedicated commercial stitching software has likewise
} improved over the last couple of years - I'll check them out as
} demos, but I
} can live with CS4's output.
}
} I've never had a problem with slightly varied light levels on a
} scientific
} montage of say a completely scanned section using Zeiss Axiovision
} and a
} motorized stage - it kinda emphasizes that it is a montage and easily
} identifies each specific image within the entire raster scan. For a
} scene of
} Tenby harbor in the real world though it does look appalling.
}
} Keith
}
} *I have to say I think Photoshop CS4 is the best update since
} Photoshop CS
} arrived.
}
} ----------------------------------------------------------------------
} -----
} Dr Keith J. Morris,
} Molecular Cytogenetics and Microscopy Core,
} Laboratory 00/069 and 00/070,
} The Wellcome Trust Centre for Human Genetics,
} Roosevelt Drive,
} Oxford OX3 7BN,
} United Kingdom.
}
} Telephone: +44 (0)1865 287568
} Email: kjmorris-at-well.ox.ac.uk
} Web-pages: http://www.well.ox.ac.uk/cytogenetics/
}
} -----Original Message-----
} X-from: Ralph Common [mailto:rcommon-at-msu.edu]
} Sent: 10 June 2009 15:22
} To: kjmorris-at-well.ox.ac.uk
} Subject: RE: [Microscopy] FW: Image stitching
}
} As I said in my original post, even CS2 does a truly seamless
} stitch of my
} LM images. The secret here, in addition to having flatfield
} objectives is,
} as you say, getting even lighting. Even my very expensive
} microscope does
} not have completely even illumination, and my camera sensor has
} brightness
} and color issues at the edges. The solution is "background
} subtraction",
} actually background division. Image Arithmetic is freeware that
} does a
} great job. You take a background shot of an empty field at the same
} exposure as your real image (in manual mode of course) and divide
} the image
} by the background image. It corrects brightness and color shifts
} remarkably
} well.
}
} You can download Image Arithmetic at http://www.t3i.nl/myblog/?
} page_id=7
}
} -----Original Message-----
} X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk]
} Sent: Wednesday, June 10, 2009 5:48 AM
} To: rcommon-at-msu.edu
} Subject: [Microscopy] FW: Image stitching
}
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Gatan include 'Digital Montage' in the software available for their
} cameras,
} that seemed to work well stitching together the TEM images [it was
} only used
} with their camera, albeit with an offline workstation]. But it was
} fantastically expensive [well the camera was and it came as a
} package]. See:
} http://ftp.gatan.com/products/digital_imaging/products/
} Digital_Montage.php
} Not used it since I left UCL a couple of years ago though, but it
} went down
} well with the TEM users [the software balances intensities between
} images as
} well]. The Montage image files were large though.
}
} I always found Photoshop CS2/CS3 Photomerge to be pretty awful at
} photo-stitching photographs and I've long abandoned it [I'll try
} the CS4
} version next time to see if it has improved any]. In fact the
} software that
} came with my camera* 'Olympus Master' [after you paid for the Pro
} upgrade]
} worked far better [for camera photos] so I still stick with that.
} It can't
} get it right every time and so a manual edit to taste is often
} required
} [camera lens distortions and positional errors are presumably worse
} than
} that found in 'flat' 2D TEM images]. Try things like: copy & paste
} badly
} affected areas, and Free Transform, and Transform 'Distort' etc. to
} overcome
} poor stitching at the overlap [plus see the link below]. Image
} analysis
} packages like MetaMorph & AxioVision seem to go for the more
} conservative
} Montage, where the 'overlap' edge is left as is, but I don't see much
} problem ethically with very minor photo editing to get it to look nice
} assuming nothing of importance is edited away or added [generally
} though
} 'looking nice' is just for the web or presentations, being
} aesthetics and
} often of no particular scientific importance].
}
} You can buy various similar photo-stitching panorama software, all
} largely
} aimed at photographers though:
}
} http://www.cs.ubc.ca/~mbrown/autostitch/autostitch.html
} [seems well regarded]
}
} http://www.ptgui.com/info/photo_stitching.html
}
} http://www.vrtoolbox.com
}
} http://www.easypano.com/photo-stitch-software.html
}
} http://www.panobuilder.com/index.asp
}
} http://www.vextrasoft.com/rasterstitch.htm
}
} This is just a selection, and prices vary [and all can't perform
} miracles,
} although the results can be very impressive after a minor edit or
} smudge
} here and there]. I tried a few on demo, but my Olympus E500 SLR
} camera's
} Olympus Pro* software did as well so I never bothered 'upgrading'.
} Often
} it's brightness differences rather than stitching that really ruins
} things,
} so camera lock on the same area of the view and move to the next
} one in
} sequence] - likewise on the microscope keep exposure times as
} identical as
} possible [I'm an optical microscope guy]. Better photo-stitching
} software
} should attempt to correct uneven lighting.
}
} If you have a decent digital camera, though do try panoramas as
} fantastic
} ones are really easy to create days [although it does mean I take
} around
} 12,000 photos a year these days]. Plus the software to do it should
} have
} come free with the camera.
}
}
} Keith
}
} E.g. Typical photo stitch help guide
} http://www.phong.com/tutorials/photostitch
}
} *e.g. Canon cameras offer similar free photo-stitch software that
} is highly
} regarded.
}
} ----------------------------------------------------------------------
} -----
} Dr Keith J. Morris,
} Molecular Cytogenetics and Microscopy Core,
} Laboratory 00/069 and 00/070,
} The Wellcome Trust Centre for Human Genetics,
} Roosevelt Drive,
} Oxford OX3 7BN,
} United Kingdom.
}
} Telephone: +44 (0)1865 287568
} Email: kjmorris-at-well.ox.ac.uk
} Web-pages: http://www.well.ox.ac.uk/cytogenetics/
}
} -----Original Message-----
} X-from: reinhard.rachel-at-biologie.uni-regensburg.de
} [mailto:reinhard.rachel-at-biologie.uni-regensburg.de]
} Sent: 05 June 2009 22:50
} To: kjmorris-at-well.ox.ac.uk
} Subject: [Microscopy] Image stitching
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} }
} ----------------------------------------------------------------------
} ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------
} ------
} }
} }
} } Photoshop CS2's Photomerge function works well on my LM flatfield
} } images,
} } but with TEM images sometimes there are misalignments in some
} } areas. I
} } assume this is due to spherical aberration in the TEM images. Can
} } any of
} } the other software options out there detect and correct for spherical
} } aberration?
} }
} } Ralph Common
} } Michigan State University
}
} In our lab, people now use PS CS4, with - I assume - further improved
} function(s) for image stitching / merging. At least, the results
} are very
} good. - Photomerge.
} TEM: this very much depends on the magnification. It works +/-
} easily and
} automatically (Photomerge) with images taken in medium / high mag mode
} (above 3000 x). In low mag mode on our CM12, images contain obvious
} distortions, preventing perfect alignments. - People who are well
} trained in
} PS CS4 can use built-in functions for correcting these distortions,
} before
} the images are merged. Not easy, but works satisfactorily.
} I just checked PS CS4 again: "Photomerge" even includes filters
} ("layout")
} offering some preliminary correction of distortions.
} HTH - best regards - Reinhard
}
}
} --
}
} PD Dr. Reinhard Rachel
} Universitaet Regensburg
} Centre for EM - NWF III -
} -at-Institute for Anatomy
} Universitaetsstr. 31
} D-93053 Regensburg - Germany
} tel +49 941 943 2837, 1720
} fax +49 941 943 2868
} mail reinhard.rachel-at-biologie.uni-regensburg.de
} office: VKL 3.1.29
}
}
}
} ==============================Original
} Headers==============================
} 7, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jun 5
} 16:44:17
} 2009
} 7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de
} (rrzmta1.rz.uni-regensburg.de [194.94.155.51])
} 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n55LiGDG001162
} 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 16:44:16
} -0500
} 7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost
} [127.0.0.1])
} 7, 26 -- by localhost (Postfix) with SMTP id 3FC06ABFAD
} 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
} +0200 (CEST)
} 7, 26 -- Received: from gwsmtp1.uni-regensburg.de
} (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
} 7, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id
} 37693ABFD0
} 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 23:44:26
} +0200 (CEST)
} 7, 26 -- Received: from uni-regensburg-smtp1-MTA by
} gwsmtp1.uni-regensburg.de
} 7, 26 -- with Novell_GroupWise; Fri, 05 Jun 2009 23:44:15 +0200
} 7, 26 -- Message-Id: {4A29ADC802000054000161F6-at-gwsmtp1.uni-
} regensburg.de}
} 7, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0
} 7, 26 -- Date: Fri, 05 Jun 2009 23:44:08 +0200
} 7, 26 -- From: "reinhard rachel"
} {reinhard.rachel-at-biologie.uni-regensburg.de}
} 7, 26 -- To: {rcommon-at-msu.edu}
} 7, 26 -- Cc: {Microscopy-at-microscopy.com}
} 7, 26 -- Subject: Image stitching
} 7, 26 -- References: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
} 7, 26 -- In-Reply-To: {200906052025.n55KPAg9018186-at-ns.microscopy.com}
} 7, 26 -- Mime-Version: 1.0
} 7, 26 -- Content-Type: text/plain; charset=US-ASCII
} 7, 26 -- Content-Disposition: inline
} 7, 26 -- Content-Transfer-Encoding: 8bit
} 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n55LiGDG001162
} ==============================End of -
} Headers==============================
}
}
} ==============================Original
} Headers==============================
} 29, 20 -- From kjmorris-at-well.ox.ac.uk Wed Jun 10 04:44:24 2009
} 29, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk
} [129.67.44.2])
} 29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id
} n5A9iNkO010297
} 29, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Jun 2009 04:44:23
} -0500
} 29, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178]
} helo=CytoWhizz)
} 29, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
} 29, 20 -- id 1MEKM3-000728-Ec
} 29, 20 -- for Microscopy-at-Microscopy.Com; Wed, 10 Jun 2009 10:44:23
} +0100
} 29, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
} 29, 20 -- To: {Microscopy-at-Microscopy.Com}
} 29, 20 -- Subject: FW: [Microscopy] Image stitching
} 29, 20 -- Date: Wed, 10 Jun 2009 10:44:24 +0100
} 29, 20 -- Message-ID: {DE2F92C5E09C4899804B1A678E319D89-at-CytoWhizz}
} 29, 20 -- MIME-Version: 1.0
} 29, 20 -- Content-Type: text/plain;
} 29, 20 -- charset="us-ascii"
} 29, 20 -- Content-Transfer-Encoding: 7bit
} 29, 20 -- X-Mailer: Microsoft Office Outlook 11
} 29, 20 -- Thread-Index: AcnmJ5S4eGjfMuLRTraVkz1lbHzamwCyPRQgAC
} +WBaAAAAuL4A==
} 29, 20 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.5579
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 48, 22 -- From kjmorris-at-well.ox.ac.uk Thu Jun 11 11:43:26 2009
} 48, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk
} [129.67.44.2])
} 48, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5BGhPHg027550
} 48, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 11 Jun 2009
} 11:43:26 -0500
} 48, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178]
} helo=CytoWhizz)
} 48, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
} 48, 22 -- id 1MEnN7-0004lf-9w
} 48, 22 -- for Microscopy-at-Microscopy.Com; Thu, 11 Jun 2009 17:43:25
} +0100
} 48, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
} 48, 22 -- To: {Microscopy-at-Microscopy.Com}
} 48, 22 -- References:
} {200906100948.n5A9mLde016355-at-ns.microscopy.com} {00c401c9e9d6
} $ddd9ee50$7d7a0923-at-msu.edu}
} 48, 22 -- In-Reply-To: {00c401c9e9d6$ddd9ee50$7d7a0923-at-msu.edu}
} 48, 22 -- Subject: RE: [Microscopy] FW: Image stitching
} 48, 22 -- Date: Thu, 11 Jun 2009 17:43:25 +0100
} 48, 22 -- Message-ID: {001501c9eab3$bd585c60$38091520$-at-ox.ac.uk}
} 48, 22 -- MIME-Version: 1.0
} 48, 22 -- Content-Type: text/plain;
} 48, 22 -- charset="US-ASCII"
} 48, 22 -- Content-Transfer-Encoding: 7bit
} 48, 22 -- X-Mailer: Microsoft Office Outlook 12.0
} 48, 22 -- thread-index: Acnp1v35dvQPTfH/TX6btz39f8PDOAAn2ZEw
} 48, 22 -- Content-Language: en-gb
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
8, 22 -- From Elliott-at-arizona.edu Thu Jun 11 12:08:21 2009
8, 22 -- Received: from mailgator.email.arizona.edu (pacer.email.arizona.edu [128.196.133.172])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BH8KPs009750
8, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 11 Jun 2009 12:08:20 -0500
8, 22 -- Received: from mailgators_amavis (amavis1.email.arizona.edu [10.0.0.204])
8, 22 -- by mailgator.email.arizona.edu (Postfix) with ESMTP id 029AC30D6FF3
8, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 11 Jun 2009 10:08:18 -0700 (MST)
8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126])
8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4922FAB7BE0
8, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Thu, 11 Jun 2009 10:08:14 -0700 (MST)
8, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1)
8, 22 -- In-Reply-To: {200906111648.n5BGm68q031931-at-ns.microscopy.com}
8, 22 -- References: {200906111648.n5BGm68q031931-at-ns.microscopy.com}
8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
8, 22 -- Message-Id: {75F2BF73-7B83-431C-9A06-977E6C110AEA-at-arizona.edu}
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- From: David Elliott {Elliott-at-arizona.edu}
8, 22 -- Subject: Re: [Microscopy] RE: FW: Image stitching
8, 22 -- Date: Thu, 11 Jun 2009 10:08:16 -0700
8, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com}
8, 22 -- X-Mailer: Apple Mail (2.753.1)
8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: schaffne-at-patho.unibe.ch
Date: Thu, 11 Jun 2009 18:19:32 -0500
Subject: [Microscopy] viaWWW: Lowicryl low-temperature embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both schaffne-at-patho.unibe.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: schaffne-at-patho.unibe.ch
Name: Thomas Schaffner

Organization: Institute of Pathology University of Bern

Title-Subject: [Filtered] Lowicryl low-temperature embedding

Question: Before considering low temperature embedding one has to
decide about sample fixation/dehydration from the intended goals of
the study. Equipment for freeze substitution i.e. real snap frozen
tissue with little distortion by ice crystals or water/ion migration
within the tissue would consist of at least a polished silver plate
chilled with liquid nitrogen in a dry atmosphere for contact-freezing
of tissue (tiny samples). Such samples are then transferred to
isopentane chilled in a -70 to -80 centigrade freezer for slow
(week)substitution of water by isopentane (which itself should just
not freeze), thereafter the Lowicryl intermedia can be applied as
indicated by the application note coming with the Lowicryl even at
higher temperatures. methylmethacrylates or hydrophilic acrylates can
also be used with appropriate catalysts and UV-irradiation in the
cold.
In my opinion the outlined procedures are tedious, prone to artefacts
and only worthwile when special questions arise.
Good luck!


Login Host: 130.92.9.55
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 18:19:31 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BNJUfW007167
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 18:19:31 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c65741721511-at-[206.69.208.22]}
7, 11 -- Date: Thu, 11 Jun 2009 18:19:28 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: schaffne-at-patho.unibe.ch (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Lowicryl low-temperature embedding
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ron.kelley-at-hp.com
Date: Thu, 11 Jun 2009 18:20:25 -0500
Subject: [Microscopy] viaWWW: EDS detector (sans electronics) for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ron.kelley-at-hp.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ron.kelley-at-hp.com
Name: Ron Kelley

Organization: Hewlett Packard

Title-Subject: [Filtered] EDS detector (sans electronics) for donation

Question: We have an older Oxford EDS detector (SiLi) from a
decomissioned ESEM 2020 WITHOUT the computer/electronics that's
available for donation (qualified group). It's model is 6807 10mm2
ATW1 with a retrograde angle of incline - specific to ESEM 2020. It
was in working order when pulled off the tool ~ 5 years ago. Payment
for shipping not included. Any takers?

Login Host: 15.251.169.70
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 18:20:25 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5BNKOql007961
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009 18:20:24 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c65741981dee-at-[206.69.208.22]}
6, 11 -- Date: Thu, 11 Jun 2009 18:20:23 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: ron.kelley-at-hp.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: EDS detector (sans electronics) for donation
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: walter.bobrowski-at-pfizer.com
Date: Fri, 12 Jun 2009 07:56:31 -0500
Subject: [Microscopy] viaWWW: TEM of embedded nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both walter.bobrowski-at-pfizer.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: walter.bobrowski-at-pfizer.com
Name: Walter Bobrowski

Organization: MSA

Title-Subject: [Filtered] TEM of embedded nanoparticles

Question: Any and all assistance greatly appreciated with imaging
nanoparticles (~20-50nm) embedded in LR White (nano drug material
encased in a spray dispersion particle). Unstained thin sections
imaged in TEM.

1. Are TEM parameters different for imaging crystalline particles
than imaging biological material? I simply cannot get sharp images of
these nanoparticles, unlike routine biological material.

2. Is there a visual way to determine if thin-sectioned nanoparticle
is amorphous or crystalline in nature?

TIA!
Walt

Login Host: 148.168.40.4
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri Jun 12 07:56:31 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CCuUfq029276
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 07:56:30 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c65800c5ed67-at-[206.69.208.22]}
9, 11 -- Date: Fri, 12 Jun 2009 07:56:29 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: walter.bobrowski-at-pfizer.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: TEM of embedded nanoparticles
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: underwoo-at-u.washington.edu
Date: Fri, 12 Jun 2009 11:11:51 -0500
Subject: [Microscopy] :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

I desire feedback on differences in immunolabeling patterns as observed between light level cryosections and cryothin sections for transmission electron microscopy. If I take the exact same tissue, a piece of skin in this case, fix it in the same fixative, usually a recipe containing buffered 2% para / 0.025% glut, for the same fixation time, cryoprotect in sucrose for LM and PVP/sucrose for TEM, then freeze and section 6 micron frozen sections for LM and 100nm thick cryothin sections picked up on nickel formvar/carbon grids for TEM, I get a completely different labeling pattern with perhaps 10-20% of antibodies. Has anyone had similar observations? If so, have you figured out why? I have tried to rule out PVP (polyvinylpyrrolidone) as one of the obvious differences between the two protocols but tissue cryoprotected with PVP/sucrose cannot be cut at 6 microns in a cryostat. I have tried to
rule out all other differences and remain stumped. As example, labeling for a particular transcription factor shows compartmentalized staining in the nucleus at the LM level and at the TEM level specifically stains intercellular desmosomal adhesions between the keratinocytes of the epidermis. Crazy!

Sincerely,
Robert Underwood
University of Washington
Dermatology
Seattle, WA







==============================Original Headers==============================
9, 22 -- From underwoo-at-u.washington.edu Fri Jun 12 11:11:51 2009
9, 22 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CGBmnS018118
9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 11:11:50 -0500
9, 22 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103])
9, 22 -- by mxout3.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5CGBj0l020228
9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:47 -0700
9, 22 -- Received: from localhost (localhost [127.0.0.1])
9, 22 -- by hymn13.u.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) with ESMTP id n5CGBgSq019694
9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:42 -0700
9, 22 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Fri, 12 Jun 2009 09:11:42 PDT
9, 22 -- Date: Fri, 12 Jun 2009 09:11:42 -0700 (PDT)
9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
9, 22 -- To: Microscopy List {Microscopy-at-microscopy.Com}
9, 22 -- Subject: [Microscopy]:LM vs TEM immunostain patterns
9, 22 -- Message-ID: {Pine.LNX.4.43.0906120911420.10631-at-hymn13.u.washington.edu}
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
9, 22 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.6.12.160138
9, 22 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
9, 22 -- SUPERLONG_LINE 0.05, BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0'
==============================End of - Headers==============================




From: drteddunne-at-yahoo.com
Date: Fri, 12 Jun 2009 12:12:00 -0500
Subject: [Microscopy] viaWWW: Checking eyepiece and objective magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hope I have the correct answer.

If you look at a 1mm x 1mm square (which is 1 sq mm) with a x10 magnification it will apear as 10 sq mm, which is approximately 3.16 x 3.16mm.

If it were to be 10 x 10mm that would be 100 sq mm which is x100 and not x10.

In other words the magnification factor which is printed on the lenses is for the area magnification and not the linear measurement.

You can use an eyepiece graticule to check magnification.

I will have a red face if this is wrong!!

Ted Dunn
The EMscope Company, Thailand



----- Original Message ----
X-from: "klr-at-mts.net" {klr-at-mts.net}
To: drteddunne-at-yahoo.com
Sent: Thursday, June 11, 2009 6:03:07 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both klr-at-mts.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: klr-at-mts.net
Name: K Ringland

Title-Subject: [Filtered] Checking eyepiece and objective magnification

Question: I recently purchased a stero-dissecting microscope
secondhand, however I am having trouble believing that the
magnifications stamped on the eyepieces and objectives are true. ie
1mm by 1mm grid square does not appear to be 10mm by 10mm when I use
a 10x eyepiece and with the 1x objective, the same is true when I use
a 10x eyepiece with a 3x objective, the square does not appear 30x
bigger. Is there a way that I can check or determine the
magnification of the objectives/eyepieces? Is there an equation out
there that might help me out. Thanks.

Login Host: 205.200.7.18
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 17:59:58 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5AMxv8T031647
5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 17:59:58 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c655eb58e568-at-[206.69.208.22]}
5, 11 -- Date: Wed, 10 Jun 2009 17:59:56 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: klr-at-mts.net (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Checking eyepiece and objective magnification
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================





==============================Original Headers==============================
24, 23 -- From drteddunne-at-yahoo.com Fri Jun 12 12:12:00 2009
24, 23 -- Received: from web33401.mail.mud.yahoo.com (web33401.mail.mud.yahoo.com [68.142.206.133])
24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5CHC05b008165
24, 23 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 12:12:00 -0500
24, 23 -- Received: (qmail 9233 invoked by uid 60001); 12 Jun 2009 17:11:58 -0000
24, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1244826718; bh=nRtwjzSVYnwhS/mIxB1yI6r0RxtmSk0O3ISXQU6uHcw=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type; b=tbE8B9CJwSL29Zh/qne+DanNPiaVBtVN5TLH7T3mIQ3BkOU8Nu4LsNfoHii7TbMFQMIWKX0SlshRzf0qF8g9NZsiOuiaIdUtHD2IlEjeI8cx4Tih3lpwdeK+uGGZEj0tX8XqCTUPhKRR76HjaX4rJLHF4EFr6vJosSrtd/9+9vU=
24, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
24, 23 -- s=s1024; d=yahoo.com;
24, 23 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type;
24, 23 -- b=MIoAKKu2KcdLn569sRvPehj9+UYRjojErB+bRQV6aXlsD8fTT9l+7ojpg6FXnvdQ4mc9/uJ0qPmk3fj78R3RK9HdFs4uO5Ygo7bRMbgR9Cyfki0xrHGAEKsh/JDP6Op1ApweR5W0uMWVaRkUJLMtxoxiTYIdKaVCTePlyQgGEtk=;
24, 23 -- Message-ID: {658634.6483.qm-at-web33401.mail.mud.yahoo.com}
24, 23 -- X-YMail-OSG: TtxcDXMVM1nSXZMYFcbN6CLAPJseSgWxmbc_vUH2nXqBo7sTPEECycZSOlIRYTlfIWf7WE.0PknePOkDfXTSBXOw2IPQ6so7pdTeoZFLkjE2hEaKrah0w1pewJ8Xl33sqpAkDKRLb0cU5lcI_NQlA03FheK6M1asafiNyQ9wqHXIGPKS0Y0FCGeau5nPauXyKS43nHDPRZgm5gJnTpIEIHV_bU6NAUNYj15TGXs4wVZExolxS2W21GwY0yLZPdYY0fb1.C83mQzw6pZcCUWm.EmfzgWSDDEZuVZPLnlkScHbjVViZiXkgQ5RwWuDizvs2vAlx4lA6xjTSjodhyiLVu7m
24, 23 -- Received: from [124.157.245.87] by web33401.mail.mud.yahoo.com via HTTP; Fri, 12 Jun 2009 10:11:58 PDT
24, 23 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.15
24, 23 -- References: {200906102303.n5AN36W2010177-at-ns.microscopy.com}
24, 23 -- Date: Fri, 12 Jun 2009 10:11:58 -0700 (PDT)
24, 23 -- From: ted dunn {drteddunne-at-yahoo.com}
24, 23 -- Subject: Re: [Microscopy] viaWWW: Checking eyepiece and objective magnification
24, 23 -- To: klr-at-mts.net
24, 23 -- Cc: microscopy-at-microscopy.com
24, 23 -- In-Reply-To: {200906102303.n5AN36W2010177-at-ns.microscopy.com}
24, 23 -- MIME-Version: 1.0
24, 23 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 12 Jun 2009 12:35:41 -0500
Subject: [Microscopy] viaWWW: Checking eyepiece and objective magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ted,
Sorry, but you're wrong. It is the linear (1D) dimensions that reflect the
magnification. My 1000 mesh gold grid (1000 holes/linear inch) shows 10
holes/linear inch at 100X or 1 hole/linear inch at 1000X.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: drteddunne-at-yahoo.com [mailto:drteddunne-at-yahoo.com]
Sent: Friday, June 12, 2009 1:18 PM
To: kenconverse-at-qualityimages.biz


I hope I have the correct answer.

If you look at a 1mm x 1mm square (which is 1 sq mm) with a x10
magnification it will apear as 10 sq mm, which is approximately 3.16 x
3.16mm.

If it were to be 10 x 10mm that would be 100 sq mm which is x100 and not
x10.

In other words the magnification factor which is printed on the lenses is
for the area magnification and not the linear measurement.

You can use an eyepiece graticule to check magnification.

I will have a red face if this is wrong!!

Ted Dunn
The EMscope Company, Thailand



----- Original Message ----
X-from: "klr-at-mts.net" {klr-at-mts.net}
To: drteddunne-at-yahoo.com
Sent: Thursday, June 11, 2009 6:03:07 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both klr-at-mts.net as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: klr-at-mts.net
Name: K Ringland

Title-Subject: [Filtered] Checking eyepiece and objective magnification

Question: I recently purchased a stero-dissecting microscope
secondhand, however I am having trouble believing that the
magnifications stamped on the eyepieces and objectives are true. ie
1mm by 1mm grid square does not appear to be 10mm by 10mm when I use
a 10x eyepiece and with the 1x objective, the same is true when I use
a 10x eyepiece with a 3x objective, the square does not appear 30x
bigger. Is there a way that I can check or determine the
magnification of the objectives/eyepieces? Is there an equation out
there that might help me out. Thanks.

Login Host: 205.200.7.18
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 17:59:58 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5AMxv8T031647
5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 17:59:58
-0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c655eb58e568-at-[206.69.208.22]}
5, 11 -- Date: Wed, 10 Jun 2009 17:59:56 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: klr-at-mts.net (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Checking eyepiece and objective magnification
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================





==============================Original Headers==============================
24, 23 -- From drteddunne-at-yahoo.com Fri Jun 12 12:12:00 2009
24, 23 -- Received: from web33401.mail.mud.yahoo.com
(web33401.mail.mud.yahoo.com [68.142.206.133])
24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n5CHC05b008165
24, 23 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 12:12:00
-0500
24, 23 -- Received: (qmail 9233 invoked by uid 60001); 12 Jun 2009 17:11:58
-0000
24, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1244826718; bh=nRtwjzSVYnwhS/mIxB1yI6r0RxtmSk0O3ISXQU6uHcw=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:C
c:In-Reply-To:MIME-Version:Content-Type;
b=tbE8B9CJwSL29Zh/qne+DanNPiaVBtVN5TLH7T3mIQ3BkOU8Nu4LsNfoHii7TbMFQMIWKX0Sls
hRzf0qF8g9NZsiOuiaIdUtHD2IlEjeI8cx4Tih3lpwdeK+uGGZEj0tX8XqCTUPhKRR76HjaX4rJL
HF4EFr6vJosSrtd/9+9vU=
24, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
24, 23 -- s=s1024; d=yahoo.com;
24, 23 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:C
c:In-Reply-To:MIME-Version:Content-Type;
24, 23 --
b=MIoAKKu2KcdLn569sRvPehj9+UYRjojErB+bRQV6aXlsD8fTT9l+7ojpg6FXnvdQ4mc9/uJ0qP
mk3fj78R3RK9HdFs4uO5Ygo7bRMbgR9Cyfki0xrHGAEKsh/JDP6Op1ApweR5W0uMWVaRkUJLMtxo
xiTYIdKaVCTePlyQgGEtk=;
24, 23 -- Message-ID: {658634.6483.qm-at-web33401.mail.mud.yahoo.com}
24, 23 -- X-YMail-OSG:
TtxcDXMVM1nSXZMYFcbN6CLAPJseSgWxmbc_vUH2nXqBo7sTPEECycZSOlIRYTlfIWf7WE.0Pkne
POkDfXTSBXOw2IPQ6so7pdTeoZFLkjE2hEaKrah0w1pewJ8Xl33sqpAkDKRLb0cU5lcI_NQlA03F
heK6M1asafiNyQ9wqHXIGPKS0Y0FCGeau5nPauXyKS43nHDPRZgm5gJnTpIEIHV_bU6NAUNYj15T
GXs4wVZExolxS2W21GwY0yLZPdYY0fb1.C83mQzw6pZcCUWm.EmfzgWSDDEZuVZPLnlkScHbjVVi
ZiXkgQ5RwWuDizvs2vAlx4lA6xjTSjodhyiLVu7m
24, 23 -- Received: from [124.157.245.87] by web33401.mail.mud.yahoo.com via
HTTP; Fri, 12 Jun 2009 10:11:58 PDT
24, 23 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.15
24, 23 -- References: {200906102303.n5AN36W2010177-at-ns.microscopy.com}
24, 23 -- Date: Fri, 12 Jun 2009 10:11:58 -0700 (PDT)
24, 23 -- From: ted dunn {drteddunne-at-yahoo.com}
24, 23 -- Subject: Re: [Microscopy] viaWWW: Checking eyepiece and objective
magnification
24, 23 -- To: klr-at-mts.net
24, 23 -- Cc: microscopy-at-microscopy.com
24, 23 -- In-Reply-To: {200906102303.n5AN36W2010177-at-ns.microscopy.com}
24, 23 -- MIME-Version: 1.0
24, 23 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




==============================Original Headers==============================
36, 25 -- From kenconverse-at-qualityimages.biz Fri Jun 12 12:35:41 2009
36, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
36, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CHZeiw024347
36, 25 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 12:35:40 -0500
36, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
36, 25 -- with ESMTP
36, 25 -- id {20090612173539855.XPKD16114-at-cdptpa-omta04.mail.rr.com} ;
36, 25 -- Fri, 12 Jun 2009 17:35:39 +0000
36, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
36, 25 -- To: {drteddunne-at-yahoo.com} , "MSA Listserver" {microscopy-at-microscopy.com}
36, 25 -- Subject: RE: [Microscopy] Re: viaWWW: Checking eyepiece and objective magnification
36, 25 -- Date: Fri, 12 Jun 2009 13:35:35 -0400
36, 25 -- Message-ID: {11A55CD6012742D48D68EBA9C4D4E0E2-at-Ken}
36, 25 -- MIME-Version: 1.0
36, 25 -- Content-Type: text/plain;
36, 25 -- charset="us-ascii"
36, 25 -- X-Priority: 3 (Normal)
36, 25 -- X-MSMail-Priority: Normal
36, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
36, 25 -- In-Reply-To: {200906121717.n5CHHY2G012438-at-ns.microscopy.com}
36, 25 -- Importance: Normal
36, 25 -- Thread-Index: AcnrgbXKlSOKCU6XTnyJtXJk1cxe4wAAcT/g
36, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
36, 25 -- Content-Transfer-Encoding: 8bit
36, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5CHZeiw024347
==============================End of - Headers==============================




From: underwoo-at-u.washington.edu
Date: Fri, 12 Jun 2009 14:56:34 -0500
Subject: [Microscopy] viaWWW: LM vs TEM IHC pattern changes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both underwoo-at-u.washington.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: underwoo-at-u.washington.edu
Name: Robert Underwood

Organization: University of Washington

Title-Subject: [Filtered] LM vs TEM IHC pattern changes

Question: Dear Microscopists,

I desire feedback on differences in immunolabeling patterns as
observe between light level cryosections and cryothin sections for
transmission electron microscopy. If I take the exact same tissue, a
piece of skin in this case, fix it in the same fixative, usually a
recipe containing buffered 2% para / 0.025% glut, for the same
fixation time, cryoprotect in sucrose for LM and PVP/sucrose for TEM,
then freeze and section 6 micron frozen sections for LM and 100nm
thick cryothin sections picked up on nickel formvar/carbon grids for
TEM, I get a completely different labeling pattern with perhaps
10-20% of antibodies. Has anyone had similar observations? If so,
have you figured out why? I have tried to rule out PVP
(polyvinylpyrrolidone) as one of the obvious differences between the
two protocols but tissue cryoprotected with PVP/sucrose cannot be cut
at 6 microns in a cryostat. I have tried to rule out all other
differences and remain stumped. As example, labeling for a particular
transcription factor shows compartmentalized staining in the nucleus
and at the TEM level specifically stains intercellular desmosomal
adhesions between the keratinocytes of the epidermis.

Sincerely,
Robert Underwood
University of Washington
Dermatology
Seattle, WA


Login Host: 128.208.106.143
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Fri Jun 12 14:56:33 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CJuWWv022047
9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 14:56:33 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c65863550791-at-[206.69.208.22]}
9, 11 -- Date: Fri, 12 Jun 2009 14:56:31 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: underwoo-at-u.washington.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: LM vs TEM IHC pattern changes
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: william.oxberry-at-downstate.edu
Date: Fri, 12 Jun 2009 14:57:15 -0500
Subject: [Microscopy] viaWWW: 473 solid state vs Argon gas laser

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both william.oxberry-at-downstate.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: william.oxberry-at-downstate.edu
Name: William Oxberry

Organization: SUNY Downstate Medical Center

Title-Subject: [Filtered] 473 solid state vs Argon gas laser

Question: We are condidering a SS diode laser with 473nm excitation
line instead of an Argon gas laser for our new confocal
system(Olympus FV-1000) Does this laser give good results for the
excitation of Alexa 488, FITC etc. ?

The Olympus rep assures me that it will. Can anybody who has
experience with this laser confirm this?

Thanks,
Bill

Login Host: 138.5.61.253
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Jun 12 14:57:14 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CJvDpn022502
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 14:57:14 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c65863770fa6-at-[206.69.208.22]}
8, 11 -- Date: Fri, 12 Jun 2009 14:57:13 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: william.oxberry-at-downstate.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: 473 solid state vs Argon gas laser
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Fri, 12 Jun 2009 15:06:32 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Robert,

Yes, I have seen this happen before, although in those case there were
always two different detection systems used. What you describe as an
example is very clear and extreme and I am not sure I have a solution.
The only thing you can do to solve this is keep all steps and
treatments rigorously the same except the visualization technique
used. Have you tried to label semi-thin cryosections prepared with the
Tokuyasu technique for LM? Using the smallest gold particles and
silver enhancement? Did you use the same buffers, additives, protocol?
Controls ok?

Even in ultrathin cryosections there is only very limited penetration
for gold conjugates when the particle size is 5 or 10 nm. You may
remember from the Atlanta workshop that such conjugates only label the
surface in general. The hydrodynamic size of LM reagents is usually
smaller than that of larger gold particles and therefore LM
secondaries penetrate more easily, so that is why the smallest
particles are preferred if you want to reach those antigens inside
hydrated sections for EM.

It seems insane when you see those differences, I agree. I can not say
that the penetration issue is the only reason, but it may be a start.
I am certain many have had similar experiences and it is very
worthwhile to understand what goes on. If not, what do we decide is
real? The label pattern we like, the one that suits us best?

Cheers, have a good weekend all!



Jan Leunissen


On 13/06/2009, at 4:12 AM, underwoo-at-u.washington.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Microscopists,
}
} I desire feedback on differences in immunolabeling patterns as
} observed between light level cryosections and cryothin sections for
} transmission electron microscopy. If I take the exact same tissue, a
} piece of skin in this case, fix it in the same fixative, usually a
} recipe containing buffered 2% para / 0.025% glut, for the same
} fixation time, cryoprotect in sucrose for LM and PVP/sucrose for
} TEM, then freeze and section 6 micron frozen sections for LM and
} 100nm thick cryothin sections picked up on nickel formvar/carbon
} grids for TEM, I get a completely different labeling pattern with
} perhaps 10-20% of antibodies. Has anyone had similar observations?
} If so, have you figured out why? I have tried to rule out PVP
} (polyvinylpyrrolidone) as one of the obvious differences between the
} two protocols but tissue cryoprotected with PVP/sucrose cannot be
} cut at 6 microns in a cryostat. I have tried to
} rule out all other differences and remain stumped. As example,
} labeling for a particular transcription factor shows
} compartmentalized staining in the nucleus at the LM level and at the
} TEM level specifically stains intercellular desmosomal adhesions
} between the keratinocytes of the epidermis. Crazy!
}
} Sincerely,
} Robert Underwood
} University of Washington
} Dermatology
} Seattle, WA
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 9, 22 -- From underwoo-at-u.washington.edu Fri Jun 12 11:11:51 2009
} 9, 22 -- Received: from mxout3.cac.washington.edu
} (mxout3.cac.washington.edu [140.142.32.166])
} 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n5CGBmnS018118
} 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 11:11:50
} -0500
} 9, 22 -- Received: from hymn13.u.washington.edu
} (hymn13.u.washington.edu [140.142.4.103])
} 9, 22 -- by mxout3.cac.washington.edu
} (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5CGBj0l020228
} 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NO)
} 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:47
} -0700
} 9, 22 -- Received: from localhost (localhost [127.0.0.1])
} 9, 22 -- by hymn13.u.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03)
} with ESMTP id n5CGBgSq019694
} 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:42
} -0700
} 9, 22 -- X-Auth-Received: from [128.208.106.143] by
} hymn13.u.washington.edu via HTTP; Fri, 12 Jun 2009 09:11:42 PDT
} 9, 22 -- Date: Fri, 12 Jun 2009 09:11:42 -0700 (PDT)
} 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
} 9, 22 -- To: Microscopy List {Microscopy-at-microscopy.Com}
} 9, 22 -- Subject: [Microscopy]:LM vs TEM immunostain patterns
} 9, 22 -- Message-ID: {Pine.LNX.4.43.0906120911420.10631-at-hymn13.u.washington.edu
} }
} 9, 22 -- MIME-Version: 1.0
} 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} 9, 22 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594,
} Antispam-Data: 2009.6.12.160138
} 9, 22 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
} 9, 22 -- SUPERLONG_LINE 0.05, BODY_SIZE_1300_1399 0,
} BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
} __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0,
} __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0,
} __TO_MALFORMED_2 0'
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
12, 20 -- From leunissen-at-aurion.nl Fri Jun 12 15:06:32 2009
12, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CK6Uk4012793
12, 20 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 15:06:30 -0500
12, 20 -- Received: from [192.168.1.50] (really [122.57.248.181]) by fep02.xtra.co.nz
12, 20 -- with ESMTP
12, 20 -- id {20090612200628.PHMS28374.fep02.xtra.co.nz-at-[192.168.1.50]} ;
12, 20 -- Sat, 13 Jun 2009 08:06:28 +1200
12, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
12, 20 -- To: underwoo-at-u.washington.edu
12, 20 -- In-Reply-To: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
12, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
12, 20 -- References: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
12, 20 -- Message-Id: {490EE65A-A1CA-4DE4-B767-912DE666A018-at-aurion.nl}
12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 20 -- Content-Transfer-Encoding: 7bit
12, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
12, 20 -- Date: Sat, 13 Jun 2009 08:06:25 +1200
12, 20 -- Cc: microscopy-at-microscopy.com
12, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Fri, 12 Jun 2009 15:51:12 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bob-
Confusing for sure.
You don't specify which "reporters" you are using in either
technique. My guess is fluorophores at the LM level and gold for EM.
Have you used peroxidase-DAB? You can use it for both preps and you
will then be comparing apples to apples in terms of marker and
labelling techniques.
Lee



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
7, 35 -- From lcgould-at-med.cornell.edu Fri Jun 12 15:51:12 2009
7, 35 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CKpBTi003765
7, 35 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 15:51:12 -0500
7, 35 -- MIME-version: 1.0
7, 35 -- Content-transfer-encoding: 7BIT
7, 35 -- Content-type: text/plain; charset=us-ascii; format=flowed
7, 35 -- Received: from mpx6.med.cornell.edu ([140.251.11.120])
7, 35 -- by mail-gw2.med.cornell.edu
7, 35 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
7, 35 -- with ESMTP id {0KL500GYB8LBNZ00-at-mail-gw2.med.cornell.edu} for
7, 35 -- microscopy-at-microscopy.com; Fri, 12 Jun 2009 16:51:11 -0400 (EDT)
7, 35 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
7, 35 -- by mpx6.med.cornell.edu
7, 35 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
7, 35 -- with ESMTPA id {0KL5009RZ8L9WB60-at-mpx6.med.cornell.edu} ; Fri,
7, 35 -- 12 Jun 2009 16:51:11 -0400 (EDT)
7, 35 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594,
7, 35 -- Antispam-Data: 2009.6.12.204049
7, 35 -- X-Perlmx-Spam: Gauge=X, Probability=10%, Report=' LINES_OF_YELLING_3 0.671,
7, 35 -- BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
7, 35 -- TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0,
7, 35 -- __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __LINES_OF_YELLING 0,
7, 35 -- __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
7, 35 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0,
7, 35 -- __TO_MALFORMED_2 0'
7, 35 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
7, 35 -- Message-id: {p06230907c6586eee5170-at-[140.251.48.23]}
7, 35 -- In-reply-to: {200906121627.n5CGRW1o032220-at-ns.microscopy.com}
7, 35 -- References: {200906121627.n5CGRW1o032220-at-ns.microscopy.com}
7, 35 -- Date: Fri, 12 Jun 2009 16:50:57 -0400
7, 35 -- To: underwoo-at-u.washington.edu,
7, 35 -- Microscopy Listserver {microscopy-at-microscopy.com}
7, 35 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
7, 35 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
==============================End of - Headers==============================




From: Rob.Bowen-at-caddock.com
Date: Fri, 12 Jun 2009 17:47:58 -0500
Subject: [Microscopy] Re: viaWWW: Au adhesion on Ti problem....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


--

Hany,

I'll speculate that the Ti is picking up oxygen, as it is wont to do,
from somewhere. Pt can form a rutile oxide similar to the Ti, so it's
adhesion wouldn't necessarily be hindered by an oxidized Ti layer. Au, not
liking oxygen, would slip off.


Robert C. Bowen
Research Scientist
Caddock Electronics, Inc
rob.bowen-at-caddock.com
http://www.caddock.com


} From: {helsayed-at-ucalgary.ca}
} Reply-To: {helsayed-at-ucalgary.ca}
} Date: Wed, 10 Jun 2009 18:05:41 -0500
} To: {rob.bowen-at-caddock.com}
} Subject: [Microscopy] viaWWW: Au adhesion on Ti problem....
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both helsayed-at-ucalgary.ca as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: helsayed-at-ucalgary.ca
} Name: Hany
}
} Organization: University of Calgary
}
} Title-Subject: [Filtered] Au adhesion on Ti problem....
}
} Question: Hi All,
}
} I have a question regarding Au thin film adhesion on Ti. We sputter a
} thin film of Ti (5 min of sputtering at 50 mA gives a Ti film of
} about 15 nm) on glass slides and follow this with sputtering a Au
} film (15 min of sputtering at 50 mA gives about 150 nm). We find that
} Au film does not stick on Ti film and that when the Au target is
} replaced by a Pt target, a Pt film adheres well to the Ti layer
} underneath. We use a Denton DV-502 A sputtering machine and the
} sputtering is done at about 8 m Torr Ar.
}
} Any suggestion of why Au does not stick on Ti film?
}
} Thanks a lot in advance
}
} Hany
}
} Login Host: 136.159.250.213
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 11 -- From zaluzec-at-microscopy.com Wed Jun 10 17:57:54 2009
} 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n5AMvrJ4027927
} 10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2009 17:57:54 -0500
} 10, 11 -- Mime-Version: 1.0
} 10, 11 -- Message-Id: {p06240801c655eae1c98a-at-[206.69.208.22]}
} 10, 11 -- Date: Wed, 10 Jun 2009 17:57:53 -0500
} 10, 11 -- To: microscopy-at-microscopy.com
} 10, 11 -- From: helsayed-at-ucalgary.ca (by way of MicroscopyListserver)
} 10, 11 -- Subject: viaWWW: Au adhesion on Ti problem....
} 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================



==============================Original Headers==============================
10, 21 -- From Rob.Bowen-at-caddock.com Fri Jun 12 17:47:58 2009
10, 21 -- Received: from msg.caddock.com (69-29-3-221.stat.centurytel.net [69.29.3.221])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CMlwHJ021504
10, 21 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 17:47:58 -0500
10, 21 -- Received: from [10.1.2.107] ([10.1.2.107])
10, 21 -- by msg.caddock.com (IceWarp 9.4.2) with ASMTP id TCH41356;
10, 21 -- Fri, 12 Jun 2009 15:47:56 -0700
10, 21 -- User-Agent: Microsoft-Entourage/11.3.3.061214
10, 21 -- Date: Fri, 12 Jun 2009 15:47:56 -0700
10, 21 -- Subject: Re: [Microscopy] viaWWW: Au adhesion on Ti problem....
10, 21 -- From: Rob Bowen {Rob.Bowen-at-caddock.com}
10, 21 -- To: {helsayed-at-ucalgary.ca} ,
10, 21 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
10, 21 -- Message-ID: {C658292C.A6F5%Rob.Bowen-at-caddock.com}
10, 21 -- Thread-Topic: [Microscopy] viaWWW: Au adhesion on Ti problem....
10, 21 -- Thread-Index: Acnrr9OuEkRP51ejEd6akQARJDSgFA==
10, 21 -- In-Reply-To: {200906102305.n5AN5fc1020430-at-ns.microscopy.com}
10, 21 -- Mime-version: 1.0
10, 21 -- Content-type: text/plain;
10, 21 -- charset="US-ASCII"
10, 21 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: binbing02-at-yahoo.com
Date: Mon, 15 Jun 2009 12:32:07 -0500
Subject: [Microscopy] plan-view TEM sample preparation by FIB?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Does anyone have experience with plan-view TEM sample preparation by using of FIB? Could you please share the info or email me some references for this?

thanks.

Sally





==============================Original Headers==============================
8, 20 -- From binbing02-at-yahoo.com Mon Jun 15 12:32:07 2009
8, 20 -- Received: from web111503.mail.gq1.yahoo.com (web111503.mail.gq1.yahoo.com [67.195.15.145])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5FHW7QH019407
8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:32:07 -0500
8, 20 -- Received: (qmail 14483 invoked by uid 60001); 15 Jun 2009 17:32:06 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245087126; bh=HNyWcO2CZaNkvyF299JE4pD0ABDty7y7l5j02T6n7C4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=HXIXK7z+a4jLkN0BzekSIalZgZHHPci2TVPIbd6rhUCEs9JoSd7GGMsd4zITGkAwNKP5ZHnNgrXb/yccIREm2QgBGekRGMPw7EaXGqGd+8rxUorgsa6EHE5m+s0NzFDmkd8OMSn3t8toljoZoPBN0cESHLXekJoQJUEJ1cA5wrM=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=0qbEtNHZZNa7c+o/TlyYtKJjzAxzEKd35f7tHMRHwteCF+bE4n+FNm94X6LtArL0TvrcCC9ZDI5RAqLgnCL58aMyJ5XrEvqludmepUrMYE1wl9FZ3vPRJkKvoS6tZ7OGYot/Qp+AdseOEE9MTnC4DBspFA7n5kIuknsNRp6lCdQ=;
8, 20 -- Message-ID: {430118.13919.qm-at-web111503.mail.gq1.yahoo.com}
8, 20 -- X-YMail-OSG: szDepHAVM1kLw3BPNJxJWmmnu7Jf_0is0le2aztZ0tq91SjY11sAG0B6wsa4R2xqqVWASNY0ssDI0Dw3gaaKjuDeq8yUJ3ntbHTa3rkKff4RCptk7fgIVhIKrj2AiPIDoeku.MC3o0Ye9whAFcTZVk71T8Xx_i6RQ2ZchUqBGqcbgi0l7oHinxliQ3rbn9YmieYA_y76TtsAoRU7AUXCciSF3C.iSMo5XL1b0W2M3yTBok6XODyNDMJKIgyGMIxNM99H4AgLPByrlWkzh4AaFhWnXlZ.imJfPNIpgWzQvAv5RZaxWA--
8, 20 -- Received: from [192.55.12.36] by web111503.mail.gq1.yahoo.com via HTTP; Mon, 15 Jun 2009 10:32:06 PDT
8, 20 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.15
8, 20 -- Date: Mon, 15 Jun 2009 10:32:06 -0700 (PDT)
8, 20 -- From: sally W {binbing02-at-yahoo.com}
8, 20 -- Subject: plan-view TEM sample preparation by FIB?
8, 20 -- To: Microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Mon, 15 Jun 2009 12:40:30 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was just watching an episode of NCIS (In infinite re-runs on USA
now...) and it dawned on me that they were using a mass spectrometer
which looked real to me- (Other things were off on their portrayal,
like timing, no specimen prep, and the results display...) and I was
just wondering if anyone out there has suffered "Lab envy" by watching
a TV show and thinking that that particular {insert lab equipment
here} would be put to much better use in your own lab...

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FHeTW9026891
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:40:29 -0500
3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 10:40:27 -0700 (PDT)
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
3, 31 -- :from:to:content-type:content-transfer-encoding;
3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
3, 31 -- b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
3, 31 -- Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
3, 31 -- :content-transfer-encoding;
3, 31 -- b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
3, 31 -- Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
3, 31 -- MIME-Version: 1.0
3, 31 -- Received: by 10.204.53.136 with SMTP id m8mr7247200bkg.109.1245087627116; Mon,
3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
3, 31 -- Message-ID: {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
3, 31 -- Subject: Lab envy?
3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
3, 31 -- To: microscopy-at-microscopy.com
3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
3, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Mon, 15 Jun 2009 14:25:27 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

When shows like NCIS, Todays Forensics, (not Forensic Files on TV that show real cases, they are correct and accurate to my knowledge to everything they show and the labs own their instruments or use contract labs) etc., run these shows with instruments they are propbably on loan. When I was with a leading MFG the MFG provided an EDXRF instrument for one of these shows and thought that this would be the price to pay for some further exposure, -- advertising, so to speak. They gave them an instrument for a few months as well as installation and application support.

Then the Hollywood or where-ever production ran a show with screen shots and results that did not exist in reality, they showed chemical atomic structure from a EDXRF acquisition, a stretch of the imagination I guess. They did not get to keep the instrument, it went back to the demo lab, I beleive, for use and re-sale.

Now if Harley would give me a Screamin Eagle to play with for six months I will try to market a motorcycle show something like American Chopperhas made their fortune with!!

Joe
---- kraftpiano-at-gmail.com wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was just watching an episode of NCIS (In infinite re-runs on USA
} now...) and it dawned on me that they were using a mass spectrometer
} which looked real to me- (Other things were off on their portrayal,
} like timing, no specimen prep, and the results display...) and I was
} just wondering if anyone out there has suffered "Lab envy" by watching
} a TV show and thinking that that particular {insert lab equipment
} here} would be put to much better use in your own lab...
}
} --Justin A. Kraft
}
} --
} "America believes in education; the average professor earns more money
} in a year than a professional athlete earns in a whole week." Evan
} Esar
}
} ==============================Original Headers==============================
} 3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
} 3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
} 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FHeTW9026891
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:40:29 -0500
} 3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 10:40:27 -0700 (PDT)
} 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
} 3, 31 -- :from:to:content-type:content-transfer-encoding;
} 3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
} 3, 31 -- b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
} 3, 31 -- Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
} 3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
} 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
} 3, 31 -- :content-transfer-encoding;
} 3, 31 -- b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
} 3, 31 -- Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
} 3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
} 3, 31 -- MIME-Version: 1.0
} 3, 31 -- Received: by 10.204.53.136 with SMTP id m8mr7247200bkg.109.1245087627116; Mon,
} 3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
} 3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
} 3, 31 -- Message-ID: {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
} 3, 31 -- Subject: Lab envy?
} 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} 3, 31 -- To: microscopy-at-microscopy.com
} 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} 3, 31 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================

--
Joe Ullmer

JoeXray LLC
7958 Dubois Road
Carlisle, OHIO 45005
OFFICE / FAX: 937 550-9224
Cell: 937 554-2628
joexray-at-cinci.rr.com
www.joexray.net

==============================Original Headers==============================
7, 21 -- From joexray-at-cinci.rr.com Mon Jun 15 14:25:27 2009
7, 21 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FJPQi9026460
7, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 14:25:26 -0500
7, 21 -- Received: from hrndva-web20-z01 ([10.128.132.111])
7, 21 -- by hrndva-smta01.mail.rr.com with ESMTP
7, 21 -- id {20090615192526217.LLWF19467-at-hrndva-smta01.mail.rr.com} ;
7, 21 -- Mon, 15 Jun 2009 19:25:26 +0000
7, 21 -- Message-ID: {20090615192526.XHES5.139420.root-at-hrndva-web20-z01}
7, 21 -- Date: Mon, 15 Jun 2009 15:25:26 -0400
7, 21 -- From: {joexray-at-cinci.rr.com}
7, 21 -- To: kraftpiano-at-gmail.com
7, 21 -- Subject: Re: [Microscopy] Lab envy?
7, 21 -- Cc: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 21 -- In-Reply-To: {200906151748.n5FHmx3E012940-at-ns.microscopy.com}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain; charset=utf-8
7, 21 -- Content-Transfer-Encoding: 7bit
7, 21 -- X-Priority: 3 (Normal)
7, 21 -- Sensitivity: Normal
7, 21 -- X-Originating-IP:
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 15 Jun 2009 16:04:37 -0500
Subject: [Microscopy] Critical point drying and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

As my coworker, tech extraordinaire Cheryl, just said, "This one is
totally wacko!"

One of our steady clients has acquired a new critical-point-dryer,
identical to ours, and they are now having charging problems on their
specimens. When they CPD in our unit, they have no charging. They have
even CPD'd one sample in theirs, one in ours, mounted them on the same
stub, coated them over here with our carbon evaporator, and viewed them
together in our FESEM. The one from their CPD charges badly, the one
from ours does not.

All aspects of specimen prep were the same. The liquid CO2 and
dehydration ethanol come from the same source, cover slips used for
mounting are identical, etc., etc., etc. The only difference is that
one specimen is CPD'd there and carried here and the other is CPD'd here
and only carried to the coater and scope.

Any thoughts? I can't wait to see the comments on this one!

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 27 -- From TindallR-at-missouri.edu Mon Jun 15 16:04:37 2009
10, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FL4aoV017116
10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 16:04:36 -0500
10, 27 -- X-IronPort-Anti-Spam-Filtered: true
10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAJdUNkrRauUo/2dsb2JhbADHPwEJhRSIUYJXgTYF
10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
10, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 15 Jun 2009 16:04:36 -0500
10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 27 -- Mon, 15 Jun 2009 16:04:36 -0500
10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 27 -- Content-class: urn:content-classes:message
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain;
10, 27 -- charset="us-ascii"
10, 27 -- Subject: Critical point drying and charging
10, 27 -- Date: Mon, 15 Jun 2009 16:04:35 -0500
10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8196-at-UM-XMAIL08.um.umsystem.edu}
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- Thread-Topic: Critical point drying and charging
10, 27 -- Thread-Index: Acnt/OM7JT4KH3/lTxKtHXww2q1fCg==
10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 27 -- To: {microscopy-at-microscopy.com}
10, 27 -- X-OriginalArrivalTime: 15 Jun 2009 21:04:36.0584 (UTC) FILETIME=[E3C7E680:01C9EDFC]
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5FL4aoV017116
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Mon, 15 Jun 2009 16:50:33 -0500
Subject: [Microscopy] Re: Critical point drying and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,
That is one for the books. Um, are you still using Tousimis?
I would call them (or have your steady client do). I think they are
pretty reputable and will help out. Sounds like that new machine is
not dehydrating properly. Could there is some grease from
manufacture, maybe, leading to a coat on the sample? Try cpd-ing a
piece of metal and see if it charges. You'd think that the carbon
coat would overcome that but maybe there is an interaction?

Have fun with this!
Tobias

}
}
} Dear Collective,
}
} As my coworker, tech extraordinaire Cheryl, just said, "This one is
} totally wacko!"
}
} One of our steady clients has acquired a new critical-point-dryer,
} identical to ours, and they are now having charging problems on their
} specimens. When they CPD in our unit, they have no charging. They have
} even CPD'd one sample in theirs, one in ours, mounted them on the same
} stub, coated them over here with our carbon evaporator, and viewed them
} together in our FESEM. The one from their CPD charges badly, the one
} from ours does not.
}
} All aspects of specimen prep were the same. The liquid CO2 and
} dehydration ethanol come from the same source, cover slips used for
} mounting are identical, etc., etc., etc. The only difference is that
} one specimen is CPD'd there and carried here and the other is CPD'd here
} and only carried to the coater and scope.
}
} Any thoughts? I can't wait to see the comments on this one!
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original Headers==============================
} 10, 27 -- From TindallR-at-missouri.edu Mon Jun 15 16:04:37 2009
} 10, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5FL4aoV017116
} 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 16:04:36 -0500
} 10, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 10, 27 -- X-IronPort-Anti-Spam-Result:
} ApoEAJdUNkrRauUo/2dsb2JhbADHPwEJhRSIUYJXgTYF
} 10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 10, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 15 Jun
} 2009 16:04:36 -0500
} 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft
} SMTPSVC(6.0.3790.3959);
} 10, 27 -- Mon, 15 Jun 2009 16:04:36 -0500
} 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 10, 27 -- Content-class: urn:content-classes:message
} 10, 27 -- MIME-Version: 1.0
} 10, 27 -- Content-Type: text/plain;
} 10, 27 -- charset="us-ascii"
} 10, 27 -- Subject: Critical point drying and charging
} 10, 27 -- Date: Mon, 15 Jun 2009 16:04:35 -0500
} 10, 27 -- Message-ID:
} {91108EF9255B394CBF8B7E3789814A4103CD8196-at-UM-XMAIL08.um.umsystem.edu}
} 10, 27 -- X-MS-Has-Attach:
} 10, 27 -- X-MS-TNEF-Correlator:
} 10, 27 -- Thread-Topic: Critical point drying and charging
} 10, 27 -- Thread-Index: Acnt/OM7JT4KH3/lTxKtHXww2q1fCg==
} 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 10, 27 -- To: {microscopy-at-microscopy.com}
} 10, 27 -- X-OriginalArrivalTime: 15 Jun 2009 21:04:36.0584 (UTC)
} FILETIME=[E3C7E680:01C9EDFC]
} 10, 27 -- Content-Transfer-Encoding: 8bit
} 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n5FL4aoV017116
} ==============================End of - Headers==============================



==============================Original Headers==============================
5, 21 -- From baskin-at-bio.umass.edu Mon Jun 15 16:50:33 2009
5, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FLoXqE003251
5, 21 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 16:50:33 -0500
5, 21 -- Received: from [172.30.52.170] (eutopia [128.119.55.30])
5, 21 -- (authenticated bits=0)
5, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n5FLoTfY011227
5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
5, 21 -- Mon, 15 Jun 2009 17:50:32 -0400 (EDT)
5, 21 -- Mime-Version: 1.0
5, 21 -- Message-Id: {p06240516c65c7184170b-at-[172.30.52.170]}
5, 21 -- In-Reply-To: {200906152105.n5FL5f8O017988-at-ns.microscopy.com}
5, 21 -- References: {200906152105.n5FL5f8O017988-at-ns.microscopy.com}
5, 21 -- Date: Mon, 15 Jun 2009 17:50:28 -0400
5, 21 -- To: TindallR-at-missouri.edu
5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
5, 21 -- Subject: Re: [Microscopy] Critical point drying and charging
5, 21 -- Cc: microscopy-at-microscopy.com
5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Mon, 15 Jun 2009 17:50:32 -0400 (EDT)
5, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Mon, 15 Jun 2009 16:55:58 -0500
Subject: [Microscopy] Re: Critical point drying and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It sounds like they forgot to get the rubber chicken.
Is your CPD facing east?
;-)
Beth

On Jun 15, 2009, at 5:05 PM, TindallR-at-missouri.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Collective,
}
} As my coworker, tech extraordinaire Cheryl, just said, "This one is
} totally wacko!"
}
} One of our steady clients has acquired a new critical-point-dryer,
} identical to ours, and they are now having charging problems on their
} specimens. When they CPD in our unit, they have no charging. They
} have
} even CPD'd one sample in theirs, one in ours, mounted them on the same
} stub, coated them over here with our carbon evaporator, and viewed
} them
} together in our FESEM. The one from their CPD charges badly, the one
} from ours does not.
}
} All aspects of specimen prep were the same. The liquid CO2 and
} dehydration ethanol come from the same source, cover slips used for
} mounting are identical, etc., etc., etc. The only difference is that
} one specimen is CPD'd there and carried here and the other is CPD'd
} here
} and only carried to the coater and scope.
}
} Any thoughts? I can't wait to see the comments on this one!
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} ==============================Original
} Headers==============================
} 10, 27 -- From TindallR-at-missouri.edu Mon Jun 15 16:04:37 2009
} 10, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n5FL4aoV017116
} 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 16:04:36 -0500
} 10, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAJdUNkrRauUo/
} 2dsb2JhbADHPwEJhRSIUYJXgTYF
} 10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 10, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 15 Jun
} 2009 16:04:36 -0500
} 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft
} SMTPSVC(6.0.3790.3959);
} 10, 27 -- Mon, 15 Jun 2009 16:04:36 -0500
} 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 10, 27 -- Content-class: urn:content-classes:message
} 10, 27 -- MIME-Version: 1.0
} 10, 27 -- Content-Type: text/plain;
} 10, 27 -- charset="us-ascii"
} 10, 27 -- Subject: Critical point drying and charging
} 10, 27 -- Date: Mon, 15 Jun 2009 16:04:35 -0500
} 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8196-at-UM-XMAIL08.um.umsystem.edu
} }
} 10, 27 -- X-MS-Has-Attach:
} 10, 27 -- X-MS-TNEF-Correlator:
} 10, 27 -- Thread-Topic: Critical point drying and charging
} 10, 27 -- Thread-Index: Acnt/OM7JT4KH3/lTxKtHXww2q1fCg==
} 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 10, 27 -- To: {microscopy-at-microscopy.com}
} 10, 27 -- X-OriginalArrivalTime: 15 Jun 2009 21:04:36.0584 (UTC)
} FILETIME=[E3C7E680:01C9EDFC]
} 10, 27 -- Content-Transfer-Encoding: 8bit
} 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n5FL4aoV017116
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
5, 21 -- From beth-at-plantbio.uga.edu Mon Jun 15 16:55:58 2009
5, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FLtvDv013989
5, 21 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 16:55:57 -0500
5, 21 -- Received: from [128.192.26.46] ([128.192.26.46])
5, 21 -- (authenticated user beth-at-plantbio.uga.edu)
5, 21 -- by dogwood.plantbio.uga.edu
5, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits));
5, 21 -- Mon, 15 Jun 2009 17:55:51 -0400
5, 21 -- Cc: microscopy microscopy {microscopy-at-microscopy.com}
5, 21 -- Message-Id: {980912C7-B777-464F-8134-9681D8B2B130-at-plantbio.uga.edu}
5, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
5, 21 -- To: TindallR-at-missouri.edu
5, 21 -- In-Reply-To: {200906152105.n5FL5k5l018091-at-ns.microscopy.com}
5, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 21 -- Content-Transfer-Encoding: 7bit
5, 21 -- Mime-Version: 1.0 (Apple Message framework v930.3)
5, 21 -- Subject: Re: [Microscopy] Critical point drying and charging
5, 21 -- Date: Mon, 15 Jun 2009 17:55:55 -0400
5, 21 -- References: {200906152105.n5FL5k5l018091-at-ns.microscopy.com}
5, 21 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Mon, 15 Jun 2009 16:56:53 -0500
Subject: [Microscopy] Re: Critical point drying and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hahahaa!

Can you put the two CPDs next to each other? Run one after the other on
the same tank? We had to do this once many years ago, but I can't remember
why. Now I have to think. Darn... Both were Tousimis and I remember that
we solved the problem, but I can't remember what that problem was.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



==============================Original Headers==============================
6, 21 -- From tina-at-pbrc.hawaii.edu Mon Jun 15 16:56:53 2009
6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FLuqar016780
6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 16:56:53 -0500
6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n5FLumAI015197
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
6, 21 -- Mon, 15 Jun 2009 11:56:49 -1000 (HST)
6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n5FLulGs015194;
6, 21 -- Mon, 15 Jun 2009 11:56:48 -1000 (HST)
6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
6, 21 -- Date: Mon, 15 Jun 2009 11:56:47 -1000 (HST)
6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
6, 21 -- X-Sender: tina-at-halia
6, 21 -- To: TindallR-at-missouri.edu
6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 21 -- Subject: Re: [Microscopy] Critical point drying and charging
6, 21 -- In-Reply-To: {200906152106.n5FL67Xo018678-at-ns.microscopy.com}
6, 21 -- Message-ID: {Pine.GSO.4.21.0906151145120.14627-100000-at-halia}
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: rosemary.white-at-csiro.au
Date: Mon, 15 Jun 2009 17:21:46 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This reminds me of the "spot-the-obvious-anomaly" in the first Jurassic Park
movie - I think it was Zeiss who offered a freebie or discount of some sort
to the first person who spotted that the compound microscope they were using
had had the condenser removed, presumably because it made the microscope
look less cluttered!

cheers,
Rosemary

On 16/06/09 5:32 AM, "joexray-at-cinci.rr.com" {joexray-at-cinci.rr.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Justin,
}
} When shows like NCIS, Todays Forensics, (not Forensic Files on TV that show
} real cases, they are correct and accurate to my knowledge to everything they
} show and the labs own their instruments or use contract labs) etc., run these
} shows with instruments they are propbably on loan. When I was with a leading
} MFG the MFG provided an EDXRF instrument for one of these shows and thought
} that this would be the price to pay for some further exposure, --
} advertising, so to speak. They gave them an instrument for a few months as
} well as installation and application support.
}
} Then the Hollywood or where-ever production ran a show with screen shots and
} results that did not exist in reality, they showed chemical atomic structure
} from a EDXRF acquisition, a stretch of the imagination I guess. They did not
} get to keep the instrument, it went back to the demo lab, I beleive, for use
} and re-sale.
}
} Now if Harley would give me a Screamin Eagle to play with for six months I
} will try to market a motorcycle show something like American Chopperhas made
} their fortune with!!
}
} Joe
} ---- kraftpiano-at-gmail.com wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I was just watching an episode of NCIS (In infinite re-runs on USA
} } now...) and it dawned on me that they were using a mass spectrometer
} } which looked real to me- (Other things were off on their portrayal,
} } like timing, no specimen prep, and the results display...) and I was
} } just wondering if anyone out there has suffered "Lab envy" by watching
} } a TV show and thinking that that particular {insert lab equipment
} } here} would be put to much better use in your own lab...
} }
} } --Justin A. Kraft
} }
} } --
} } "America believes in education; the average professor earns more money
} } in a year than a professional athlete earns in a whole week." Evan
} } Esar
} }
} } ==============================Original Headers==============================
} } 3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
} } 3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com
} } [209.85.220.212])
} } 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n5FHeTW9026891
} } 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:40:29 -0500
} } 3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
} } 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 10:40:27
} } -0700 (PDT)
} } 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} } 3, 31 -- d=gmail.com; s=gamma;
} } 3, 31 --
} } h=domainkey-signature:mime-version:received:date:message-id:subject
} } 3, 31 -- :from:to:content-type:content-transfer-encoding;
} } 3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
} } 3, 31 --
} } b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
} } 3, 31 --
} } Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
} } 3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
} } 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} } 3, 31 -- d=gmail.com; s=gamma;
} } 3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
} } 3, 31 -- :content-transfer-encoding;
} } 3, 31 --
} } b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
} } 3, 31 --
} } Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
} } 3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
} } 3, 31 -- MIME-Version: 1.0
} } 3, 31 -- Received: by 10.204.53.136 with SMTP id
} } m8mr7247200bkg.109.1245087627116; Mon,
} } 3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
} } 3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
} } 3, 31 -- Message-ID:
} } {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
} } 3, 31 -- Subject: Lab envy?
} } 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} } 3, 31 -- To: microscopy-at-microscopy.com
} } 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} } 3, 31 -- Content-Transfer-Encoding: 7bit
} } ==============================End of - Headers==============================
}
} --
} Joe Ullmer
}
} JoeXray LLC
} 7958 Dubois Road
} Carlisle, OHIO 45005
} OFFICE / FAX: 937 550-9224
} Cell: 937 554-2628
} joexray-at-cinci.rr.com
} www.joexray.net
}
} ==============================Original Headers==============================
} 7, 21 -- From joexray-at-cinci.rr.com Mon Jun 15 14:25:27 2009
} 7, 21 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com
} [71.74.56.123])
} 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n5FJPQi9026460
} 7, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 14:25:26 -0500
} 7, 21 -- Received: from hrndva-web20-z01 ([10.128.132.111])
} 7, 21 -- by hrndva-smta01.mail.rr.com with ESMTP
} 7, 21 -- id {20090615192526217.LLWF19467-at-hrndva-smta01.mail.rr.com} ;
} 7, 21 -- Mon, 15 Jun 2009 19:25:26 +0000
} 7, 21 -- Message-ID: {20090615192526.XHES5.139420.root-at-hrndva-web20-z01}
} 7, 21 -- Date: Mon, 15 Jun 2009 15:25:26 -0400
} 7, 21 -- From: {joexray-at-cinci.rr.com}
} 7, 21 -- To: kraftpiano-at-gmail.com
} 7, 21 -- Subject: Re: [Microscopy] Lab envy?
} 7, 21 -- Cc: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 21 -- In-Reply-To: {200906151748.n5FHmx3E012940-at-ns.microscopy.com}
} 7, 21 -- MIME-Version: 1.0
} 7, 21 -- Content-Type: text/plain; charset=utf-8
} 7, 21 -- Content-Transfer-Encoding: 7bit
} 7, 21 -- X-Priority: 3 (Normal)
} 7, 21 -- Sensitivity: Normal
} 7, 21 -- X-Originating-IP:
} ==============================End of - Headers==============================



==============================Original Headers==============================
6, 43 -- From prvs=41066db59=Rosemary.White-at-csiro.au Mon Jun 15 17:21:45 2009
6, 43 -- Received: from act-MTAout4.csiro.au (act-MTAout4.csiro.au [150.229.7.41])
6, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FMLhCM015760
6, 43 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 17:21:44 -0500
6, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
6, 43 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
6, 43 -- s=email; t=1245104504; x=1276640504;
6, 43 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
6, 43 -- mime-version:content-transfer-encoding:content-id:
6, 43 -- content-description:resent-date:resent-from:resent-sender:
6, 43 -- resent-to:resent-cc:resent-message-id:in-reply-to:
6, 43 -- references:list-id:list-help:list-unsubscribe:
6, 43 -- list-subscribe:list-post:list-owner:list-archive;
6, 43 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
6, 43 -- |Subject:=20Re:=20[Microscopy]=20Re:=20Lab=20envy?|Date:
6, 43 -- =20Tue,=2016=20Jun=202009=2008:23:30=20+1000|Message-ID:
6, 43 -- =20 {C65D0702.678D%rosemary.white-at-csiro.au} |To:=20 {Microsc
6, 43 -- opy-at-microscopy.com} |MIME-Version:=201.0
6, 43 -- |Content-Transfer-Encoding:=207bit|In-Reply-To:=20 {200906
6, 43 -- 151932.n5FJWP64003418-at-ns.microscopy.com} ;
6, 43 -- bh=EbuOzHdZjB3QXlycBNz3pJvu6pQXZyRwGT6HfGxy5Bw=;
6, 43 -- b=Y4zS9qntSria+OuuIWOA4r96rRZM8ZKtmQD7OFreNr+ncuaJCIshNT7l
6, 43 -- tv5HQRaIuBi+QBqNLcs8PKfj3McZ95ufDiJgPYWa03Y7NmNZNtnkFpWnw
6, 43 -- XnadfoYUTMWsegc;
6, 43 -- X-IronPort-AV: E=Sophos;i="4.42,224,1243778400";
6, 43 -- d="scan'208";a="28851025"
6, 43 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
6, 43 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 16 Jun 2009 08:21:41 +1000
6, 43 -- Received: from [152.83.193.49] (152.83.193.49) by exvic-htca01.nexus.csiro.au
6, 43 -- (138.194.81.126) with Microsoft SMTP Server id 8.1.375.2; Tue, 16 Jun 2009
6, 43 -- 08:21:40 +1000
6, 43 -- User-Agent: Microsoft-Entourage/12.10.0.080409
6, 43 -- Date: Tue, 16 Jun 2009 08:23:30 +1000
6, 43 -- Subject: Re: [Microscopy] Re: Lab envy?
6, 43 -- From: Rosemary White {rosemary.white-at-csiro.au}
6, 43 -- To: {Microscopy-at-microscopy.com}
6, 43 -- Message-ID: {C65D0702.678D%rosemary.white-at-csiro.au}
6, 43 -- Thread-Topic: [Microscopy] Re: Lab envy?
6, 43 -- Thread-Index: Acnt8AY3YOw0MWQ7Q0eUYo3gSe6l9AAF+Ll4
6, 43 -- In-Reply-To: {200906151932.n5FJWP64003418-at-ns.microscopy.com}
6, 43 -- MIME-Version: 1.0
6, 43 -- Content-Type: text/plain; charset="US-ASCII"
6, 43 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: bnross-at-interchange.ubc.ca
Date: Mon, 15 Jun 2009 17:28:40 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On the subject of incorrect timing, specimen prep, results, etc. the worst offender I have seen so far in terms of microscopy is the show Eleventh Hour. They put a hydrated sample on a microscope slide with a coverslip in a "scanning electron microscope" which was actually at best an upright widefield fluorescence microscope, or just a standard bright field optical microscope. However, to their (very slight) credit, they did show a pseudo-colored SEM image as the "result." But then they proceeded to animate it with cheesy computer graphics to make it look like the sample was moving.

Also, on the same program, I believe they showed a light microscope image as a result from a TEM. Sample prep for the TEM was also not required.

At least I got a good chuckle out of it!
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4




-----Original Message-----

} Date: Mon Jun 15 10:46:53 PDT 2009
} From: kraftpiano-at-gmail.com
} Subject: [Microscopy] Lab envy?
} To: bnross-at-interchange.ubc.ca
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was just watching an episode of NCIS (In infinite re-runs on USA
} now...) and it dawned on me that they were using a mass spectrometer
} which looked real to me- (Other things were off on their portrayal,
} like timing, no specimen prep, and the results display...) and I was
} just wondering if anyone out there has suffered "Lab envy" by watching
} a TV show and thinking that that particular {insert lab equipment
} here} would be put to much better use in your own lab...
}
} --Justin A. Kraft
}
} --
} "America believes in education; the average professor earns more money
} in a year than a professional athlete earns in a whole week." Evan
} Esar
}
} ==============================Original Headers==============================
} 3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
} 3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
} 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FHeTW9026891
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:40:29 -0500
} 3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 10:40:27 -0700 (PDT)
} 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
} 3, 31 -- :from:to:content-type:content-transfer-encoding;
} 3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
} 3, 31 -- b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
} 3, 31 -- Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
} 3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
} 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
} 3, 31 -- :content-transfer-encoding;
} 3, 31 -- b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
} 3, 31 -- Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
} 3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
} 3, 31 -- MIME-Version: 1.0
} 3, 31 -- Received: by 10.204.53.136 with SMTP id m8mr7247200bkg.109.1245087627116; Mon,
} 3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
} 3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
} 3, 31 -- Message-ID: {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
} 3, 31 -- Subject: Lab envy?
} 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} 3, 31 -- To: microscopy-at-microscopy.com
} 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} 3, 31 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-6996


==============================Original Headers==============================
12, 26 -- From bnross-at-interchange.ubc.ca Mon Jun 15 17:28:40 2009
12, 26 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FMSdRe027675
12, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 17:28:40 -0500
12, 26 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
12, 26 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP id 9EFD1156FB
12, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 15:28:38 -0700 (PDT)
12, 26 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12])
12, 26 -- by smtp.interchange.ubc.ca
12, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
12, 26 -- with ESMTP id {0KLA000K7X3PUU-at-smtp.interchange.ubc.ca} for
12, 26 -- Microscopy-at-microscopy.com; Mon, 15 Jun 2009 15:28:38 -0700 (PDT)
12, 26 -- Date: Mon, 15 Jun 2009 15:28:37 -0700 (PDT)
12, 26 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
12, 26 -- Subject: Re: Lab envy?
12, 26 -- To: Microscopy-at-microscopy.com
12, 26 -- Message-id: {2268261.12261245104917498.JavaMail.myubc2-at-brahms.my.ubc.ca}
12, 26 -- MIME-version: 1.0
12, 26 -- X-Mailer: uPortal WEB email client 3.0
12, 26 -- Content-type: text/plain; charset=us-ascii
12, 26 -- Content-transfer-encoding: 7bit
12, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.6.15.221713
12, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
12, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0
12, 26 -- X-Spam-Level:
12, 26 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Mon, 15 Jun 2009 18:21:13 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think the TV shows do use real equipment on the sets, however, their technical advisors seem to not know what the stuff is they buy or what it's
used for.  I know for expediencies sake they leave out steps to get to the end point.  Try going from wet sample to H&E slide in 10 minutes like they did on CSI once. Not to mention that one person, Abby, could not do
everything they have her do.  Consider her a compilation character of all the goth/punk lab techs you've had over the years.

On "Dexter", they have a old Zeiss column and console just sitting in the lab.  I agree that the orange Zeiss column is pretty and it does make for pretty lab pictures besides they probably picked it up cheap at a lab supply yard sale.  Remember, blood spatter analysts always use TEM's as part of their job.

Working with a pretty orange Zeiss right now.

Paula  :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.






==============================Original Headers==============================
9, 22 -- From vapatpxs-at-yahoo.com Mon Jun 15 18:21:13 2009
9, 22 -- Received: from web46103.mail.sp1.yahoo.com (web46103.mail.sp1.yahoo.com [68.180.199.120])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5FNLCrm016053
9, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 15 Jun 2009 18:21:13 -0500
9, 22 -- Received: (qmail 59988 invoked by uid 60001); 15 Jun 2009 23:21:10 -0000
9, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245108070; bh=eSYtLK2wTfd4r/u09hfmBbhv2HqKOHI/STxl14EH6DM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=ZwGL1i+uPYukyepsEAtSYHZRGd1mjwW+z0u3+hCKiUQdFENNmlVB0q0+faqPX/iFxNTiTckdW6+WCBdW+yOAQa4ktDx2jIfxRvnQgzR+JrPkrhFNSkmXRj600ZlAASJjnYcALa7zt2QiWL1e703dX401S6HaiwoENSHPJGYjREk=
9, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
9, 22 -- s=s1024; d=yahoo.com;
9, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
9, 22 -- b=P0rrs3cCp4bl95yvq+2zVh8+uI76bCP3fhTz4j19fmA0eyA71/87xHVSn6+6EQEsWF5rcttvLFs/t68KD3G301TldZFEDzuVIXPTHDnlkNyp3gM7br4xqFBHa7joLumGJp7cg4djsRwm1/v7cvIbLpBVUzGMDwf7jdZ8cMhlkjY=;
9, 22 -- Message-ID: {573959.59829.qm-at-web46103.mail.sp1.yahoo.com}
9, 22 -- X-YMail-OSG: pkYfISoVM1lN_7upJf9Z83a7qbe4tBvw2u5SWFNg.dHQCASKw9hDo3U0xWlS3N_YrX87GUkfAd5KeOR3iCDOkGXvZQp34QxHWnODZcrMfUS.0A_SwXzmki03FnJF7iRTsZE98BeSmbEDPjJ6YFI.o.igxPN.MgB694kVXzCbx0Cwf4xAuYW.tdvWQLmiL5drN9fpaLtBmPLUy57k1pDoltPMFmmpHxw.dkBf6fcpsYOXiZ4c4nkHN8OTlFqnPcWfSb0iiOhoYHKnc0Ei5JreVxxpl85zNNJxVkLi.Q--
9, 22 -- Received: from [132.239.85.200] by web46103.mail.sp1.yahoo.com via HTTP; Mon, 15 Jun 2009 16:21:10 PDT
9, 22 -- X-Mailer: YahooMailClassic/5.4.12 YahooMailWebService/0.7.289.15
9, 22 -- Date: Mon, 15 Jun 2009 16:21:10 -0700 (PDT)
9, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
9, 22 -- Subject: Re: [Microscopy] Lab envy?
9, 22 -- To: MSA BB {Microscopy-at-Microscopy.Com}
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain; charset=iso-8859-1
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5FNLCrm016053
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Mon, 15 Jun 2009 18:24:30 -0500
Subject: [Microscopy] viaWWW: Lowicryl low-temperature embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe that at least some of the varieties of Lowicryl (e.g., K4M)
will freeze at dry ice temperatures. How you approach this partly
depends on your purpose. If you simply want the benefits of a
hydrophilic "immuno-friendly" resin, you may get away with doing your
polymerization at 4 C in a cold room. But polymerization is exothermic
and more extraction occurs at 4 C than at lower temperatures so there is
an advantage of going lower. Commercial devices are pretty expensive but
we designed a simple apparatus:
Shoemaker, W., C. Hayes, and T.E. Phillips. 2003. A simple, low-cost
device for processing and embedding tissues at sub-zero temperatures.
Microscopy Research and Technique 62:262-266. Tom Phillips


Thomas E. Phillips, Ph.D.
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
Biological Sciences
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (voice)
573-882-0123 (fax)


-----Original Message-----
X-from: dlowry-at-asu.edu [mailto:dlowry-at-asu.edu]
Sent: Thursday, June 11, 2009 8:50 AM
To: Phillips, Thomas E.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver
------------------------------------------------------------------------
---

Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] Lowicryl low-temperature embedding

Question: we are interested in attempting the use of a Lowicryl resin
for infiltrating and embedding samples at low sub-zero temperature.
How does one go about handling and processing samples at these
temperatures--does this normally require access to special
refrigerators and instruments, or are there strategies using more
common equipment and perhaps dry ice that will work? Any advice is
greatly appreciated.

Login Host: 129.219.58.218
------------------------------------------------------------------------
---

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jun 11 08:47:46 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5BDljMe029951
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jun 2009
08:47:45 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240809c656bb70ac08-at-[206.69.208.22]}
6, 11 -- Date: Thu, 11 Jun 2009 08:47:45 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: dlowry-at-asu.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Lowicryl low-temperature embedding
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
16, 29 -- From PhillipsT-at-missouri.edu Mon Jun 15 18:24:29 2009
16, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FNOTBO022043
16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 18:24:29 -0500
16, 29 -- X-IronPort-Anti-Spam-Filtered: true
16, 29 -- X-IronPort-Anti-Spam-Result: ApoEAGd1NkrRauUp/2dsb2JhbADFdwEBAQeFLIhRglcBAYE0BYhb
16, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
16, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 15 Jun 2009 18:24:29 -0500
16, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
16, 29 -- Mon, 15 Jun 2009 18:24:29 -0500
16, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5
16, 29 -- Content-class: urn:content-classes:message
16, 29 -- MIME-Version: 1.0
16, 29 -- Content-Type: text/plain;
16, 29 -- charset="us-ascii"
16, 29 -- Subject: RE: [Microscopy] viaWWW: Lowicryl low-temperature embedding
16, 29 -- Date: Mon, 15 Jun 2009 18:24:28 -0500
16, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD06CB60BD-at-UM-XMAIL06.um.umsystem.edu}
16, 29 -- In-Reply-To: {200906111349.n5BDnb54032095-at-ns.microscopy.com}
16, 29 -- X-MS-Has-Attach:
16, 29 -- X-MS-TNEF-Correlator:
16, 29 -- Thread-Topic: [Microscopy] viaWWW: Lowicryl low-temperature embedding
16, 29 -- Thread-Index: Acnqm3d0//7p+YIjT8C9UzeD0NPltADdNrsQ
16, 29 -- References: {200906111349.n5BDnb54032095-at-ns.microscopy.com}
16, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
16, 29 -- To: {dlowry-at-asu.edu} , {microscopy-at-microscopy.com}
16, 29 -- X-OriginalArrivalTime: 15 Jun 2009 23:24:29.0028 (UTC) FILETIME=[6E113A40:01C9EE10]
16, 29 -- Content-Transfer-Encoding: 8bit
16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5FNOTBO022043
==============================End of - Headers==============================




From: henrik.kaker-at-guest.arnes.si
Date: Mon, 15 Jun 2009 18:50:03 -0500
Subject: [Microscopy] viaWWW: Rotary vacuum pump for Jeol JSM35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My two favorites are from the movies, The Andromeda Strain and Predator 2.

In The Andromeda Strain, they took the virus and put it in a Philips EM300
TEM and then instantaneously got a color image and watched it in real time
multiply under the beam. Recall that movie came out in 1971. In Predator
2, the forensic scientist put the claw into the SEM, closed the door, and
instantly got an XEDS spectrum and concluded that the material wasn't from
earth because it didn't match any of the elements.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com

-----Original Message-----
X-from: bnross-at-interchange.ubc.ca [mailto:bnross-at-interchange.ubc.ca]
Sent: Monday, June 15, 2009 3:35 PM
To: swalck-at-southbaytech.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both henrik.kaker-at-guest.arnes.si as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: henrik.kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: SEM-EDS Lab, Metal Ravne

Title-Subject: [Filtered] Rotary vacuum pump for Jeol JSM35

Question: Dear All,

We are looking for reassembling procedure for rotary vacuum pump. We
have small oil leak through axis and we must replace the rubber seal.

Best regards,

Henrik

Login Host: 89.212.22.21
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Jun 15 18:50:02 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FNo1Fm027168
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 18:50:02 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c65c8e973016-at-[206.69.208.22]}
9, 11 -- Date: Mon, 15 Jun 2009 18:50:00 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: henrik.kaker-at-guest.arnes.si (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Rotary vacuum pump for Jeol JSM35
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Mon, 15 Jun 2009 18:50:35 -0500
Subject: [Microscopy] viaWWW: Coloring in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Coloring in Photoshop

Question: I would like to attempt to color two different types of
cells in an SEM image. I recall an article, somewhere, (Microscopy
Today or maybe Microscopy & Microanalysis?) about just this. I have
started to look at my saved magazines, but I have not yet come across
it. Can anyone help me out with this article, if they recall just
where it was. If I remember, it was pretty helpful, going step by
step on how to do it in Photoshop.

Thank you! Peggy

Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher-at-princeton.edu

Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Mon Jun 15 18:50:35 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FNoYSW027543
8, 11 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 18:50:34 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c65c8eb13620-at-[206.69.208.22]}
8, 11 -- Date: Mon, 15 Jun 2009 18:50:33 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Coloring in Photoshop
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lamiller-at-illinois.edu
Date: Mon, 15 Jun 2009 21:52:53 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

1978, I loved it when Quincy used a hematology cell counter, a Coulter
Counter, to do instantaneous heavy metal analysis, that took less time
than even running a cell count could take!

==============================Original Headers==============================
1, 19 -- From lamiller-at-illinois.edu Mon Jun 15 21:52:53 2009
1, 19 -- Received: from QMTA12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227])
1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5G2qrhL004785
1, 19 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 21:52:53 -0500
1, 19 -- Received: from OMTA03.emeryville.ca.mail.comcast.net ([76.96.30.27])
1, 19 -- by QMTA12.emeryville.ca.mail.comcast.net with comcast
1, 19 -- id 4QEG1c0010b6N64ACSstuM; Tue, 16 Jun 2009 02:52:53 +0000
1, 19 -- Received: from lou-anns-computer.local ([98.212.148.206])
1, 19 -- by OMTA03.emeryville.ca.mail.comcast.net with comcast
1, 19 -- id 4Sss1c00E4TRewa8PSssyC; Tue, 16 Jun 2009 02:52:53 +0000
1, 19 -- Message-ID: {4A370902.9090901-at-illinois.edu}
1, 19 -- Date: Mon, 15 Jun 2009 21:52:50 -0500
1, 19 -- From: Lou Ann Miller {lamiller-at-illinois.edu}
1, 19 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302)
1, 19 -- MIME-Version: 1.0
1, 19 -- To: microscopy-at-microscopy.com
1, 19 -- Subject: [Microscopy] Lab envy?
1, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
1, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Mon, 15 Jun 2009 22:30:40 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Scott;
Andromeda Strain used a Forgeflow, which was the same as the last RCA
TEM.

John Mardinly
Sent from my iPhone.

On Jun 15, 2009, at 4:47 PM, swalck-at-southbaytech.com wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} My two favorites are from the movies, The Andromeda Strain and
} Predator 2.
}
} In The Andromeda Strain, they took the virus and put it in a Philips
} EM300
} TEM and then instantaneously got a color image and watched it in
} real time
} multiply under the beam. Recall that movie came out in 1971. In
} Predator
} 2, the forensic scientist put the claw into the SEM, closed the
} door, and
} instantly got an XEDS spectrum and concluded that the material
} wasn't from
} earth because it didn't match any of the elements.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} Technical Director
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} US Toll Free: 1-800-728-2233
} Tel: (949) 492-2600
} Fax: (949) 492-1499
}
} www.southbaytech.com
} swalck-at-southbaytech.com
}
} -----Original Message-----
} X-from: bnross-at-interchange.ubc.ca [mailto:bnross-at-interchange.ubc.ca]
} Sent: Monday, June 15, 2009 3:35 PM
} To: swalck-at-southbaytech.com
} Subject: [Microscopy] Re: Lab envy?
}
}
}
} -----Original Message-----
}
} } Date: Mon Jun 15 10:46:53 PDT 2009
} } From: kraftpiano-at-gmail.com
} } Subject: [Microscopy] Lab envy?
} } To: bnross-at-interchange.ubc.ca
} }
} }
} }
} }
} }
} ---
} ---
} ----------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ---
} ---
} ----------------------------------------------------------------------
} }
} } I was just watching an episode of NCIS (In infinite re-runs on USA
} } now...) and it dawned on me that they were using a mass spectrometer
} } which looked real to me- (Other things were off on their portrayal,
} } like timing, no specimen prep, and the results display...) and I was
} } just wondering if anyone out there has suffered "Lab envy" by
} } watching
} } a TV show and thinking that that particular {insert lab equipment
} } here} would be put to much better use in your own lab...
} }
} } --Justin A. Kraft
} }
} } --
} } "America believes in education; the average professor earns more
} } money
} } in a year than a professional athlete earns in a whole week." Evan
} } Esar
} }
} } ==============================Original
} Headers==============================
} } 3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
} } 3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-
} } f212.google.com
} [209.85.220.212])
} } 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id
} n5FHeTW9026891
} } 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} } 12:40:29
} -0500
} } 3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
} } 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 10:40:27 -0700 (PDT)
} } 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} } 3, 31 -- d=gmail.com; s=gamma;
} } 3, 31 --
} h=domainkey-signature:mime-version:received:date:message-id:subject
} } 3, 31 -- :from:to:content-type:content-transfer-encoding;
} } 3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
} } 3, 31 --
} b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
} } 3, 31 --
} Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
} } 3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
} } 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} } 3, 31 -- d=gmail.com; s=gamma;
} } 3, 31 --
} h=mime-version:date:message-id:subject:from:to:content-type
} } 3, 31 -- :content-transfer-encoding;
} } 3, 31 --
} b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
} } 3, 31 --
} Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
} } 3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
} } 3, 31 -- MIME-Version: 1.0
} } 3, 31 -- Received: by 10.204.53.136 with SMTP id
} m8mr7247200bkg.109.1245087627116; Mon,
} } 3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
} } 3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
} } 3, 31 -- Message-ID:
} {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
} } 3, 31 -- Subject: Lab envy?
} } 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} } 3, 31 -- To: microscopy-at-microscopy.com
} } 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} } 3, 31 -- Content-Transfer-Encoding: 7bit
} } ==============================End of -
} Headers==============================
} --
} Bradford Ross
}
} Microscopy Technician
} BioImaging Facility
} University of British Columbia
} 6270 University Blvd.
} Vancouver, B.C.
} Canada
} V6T 1Z4
}
} phone 604-822-6996
}
}
} ==============================Original
} Headers==============================
} 12, 26 -- From bnross-at-interchange.ubc.ca Mon Jun 15 17:28:40 2009
} 12, 26 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca
} [137.82.45.11])
} 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id
} n5FMSdRe027675
} 12, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 17:28:40
} -0500
} 12, 26 -- Received: from mta1.interchange.ubc.ca
} (mta1.interchange.ubc.ca
} [142.103.145.69])
} 12, 26 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP id
} 9EFD1156FB
} 12, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 15:28:38
} -0700 (PDT)
} 12, 26 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12
} ])
} 12, 26 -- by smtp.interchange.ubc.ca
} 12, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8
} 2003))
} 12, 26 -- with ESMTP id {0KLA000K7X3PUU-at-smtp.interchange.ubc.ca} for
} 12, 26 -- Microscopy-at-microscopy.com; Mon, 15 Jun 2009 15:28:38
} -0700 (PDT)
} 12, 26 -- Date: Mon, 15 Jun 2009 15:28:37 -0700 (PDT)
} 12, 26 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
} 12, 26 -- Subject: Re: Lab envy?
} 12, 26 -- To: Microscopy-at-microscopy.com
} 12, 26 -- Message-id:
} {2268261.12261245104917498.JavaMail.myubc2-at-brahms.my.ubc.ca}
} 12, 26 -- MIME-version: 1.0
} 12, 26 -- X-Mailer: uPortal WEB email client 3.0
} 12, 26 -- Content-type: text/plain; charset=us-ascii
} 12, 26 -- Content-transfer-encoding: 7bit
} 12, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-
} Engine:
} 2.6.1.350677, Antispam-Data: 2009.6.15.221713
} 12, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
} 12, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05,
} BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
} ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0,
} WEBMAIL_XMAILER 0,
} __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0,
} __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0,
} __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0,
} __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0,
} __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0,
} __SANE_MSGID 0,
} __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0
} 12, 26 -- X-Spam-Level:
} 12, 26 -- X-Spam-Flag: No
} ==============================End of -
} Headers==============================
} Internal Virus Database is out of date.
} Checked by AVG - http://www.avg.com
} Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date:
} 8/15/2008
} 5:29 PM
}
}
}
}
} ==============================Original
} Headers==============================
} 14, 29 -- From swalck-at-southbaytech.com Mon Jun 15 18:40:48 2009
} 14, 29 -- Received: from n5a.bullet.mud.yahoo.com
} (n5a.bullet.mud.yahoo.com [209.191.126.232])
} 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with



From: nizets2-at-yahoo.com
Date: Tue, 16 Jun 2009 04:03:48 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Although I find the contributions of Mr Leunissen always very interesting, I don't completely agree on this one.
If 2 methods demand different approaches, just approach them differently!
Let's suppose that you are working with polyclonal antibodies, you may reasonable think that there exist a possibility for different recognition patterns.
The antigens are usually less accessible in TEM (as stated by Mr Leunissen), which usually ends up in increased antibody concentrations. These are limitations that you cannot overcome, they are inherent to the technique.
I would just optimize each technique independently.
Now on the interpretation side: Perhaps (I said perhaps) the desmosome labeling was so intense that you did not pay enough attention to any other weak labeling. If you detect say 250 particles/µm² in the desmosomes, 25 in nuclear compartments and 5 in the resin, the nuclear compartment is still specifically labeled! (although weaker)
Or perhaps the antibody concentration is enough to label the desmosomes but not enough to label the nucleus (just increase the concentration further).

You seem to have no idea what to expect for a labeling pattern. You could make a simple immunolabeling in different cells in culture, so you would get an idea of how the transcription factor is generally distributed using your antibody. (this would answer questions like "what labeling is real?")
Normal keratinocytes and skin fibroblasts are available for cell culture (you lucky you). Keratinocytes may even be easily influenced just by changing the Ca2+ concentration in culture medium. Notwithstanding the fact that you will probably be freed from any unwanted desmosome labeling.

Side note: it would be helpful (I think) to run a western blot with a cell extract and the antibody you use in microscopy. You may be able to distinguish several bands, some attributed to your specific antigen (transcription factor) and others to unspecific targets (desmosome components?).

What really troubles me is that you have a strong labeling of desmosomes in EM and none in LM (or do you?). But hey if we could explain everything we would be finders and not researchers :-)

Best regards,
Stephane



----- Original Message ----
X-from: "leunissen-at-aurion.nl" {leunissen-at-aurion.nl}
To: nizets2-at-yahoo.com
Sent: Friday, June 12, 2009 10:10:22 PM

Hello Robert,

Yes, I have seen this happen before, although in those case there were 
always two different detection systems used. What you describe as an 
example is very clear and extreme and I am not sure I have a solution. 
The only thing you can do to solve this is keep all steps and 
treatments rigorously the same except the visualization technique 
used. Have you tried to label semi-thin cryosections prepared with the 
Tokuyasu technique for LM? Using the smallest gold particles and 
silver enhancement? Did you use the same buffers, additives, protocol? 
Controls ok?

Even in ultrathin cryosections there is only very limited penetration 
for gold conjugates when the particle size is 5 or 10 nm. You may 
remember from the Atlanta workshop that such conjugates only label the 
surface in general. The hydrodynamic size of LM reagents is usually 
smaller than that of larger gold particles and therefore LM 
secondaries penetrate more easily, so that is why the smallest 
particles are preferred if you want to reach those antigens inside 
hydrated sections for EM.

It seems insane when you see those differences, I agree. I can not say 
that the penetration issue is the only reason, but it may be a start. 
I am certain many have had similar experiences and it is very 
worthwhile to understand what goes on. If not, what do we decide is 
real? The label pattern we like, the one that suits us best?

Cheers, have a good weekend all!



Jan Leunissen


On 13/06/2009, at 4:12 AM, underwoo-at-u.washington.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of 
} America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Microscopists,
}
} I desire feedback on differences in immunolabeling patterns as 
} observed between light level cryosections and cryothin sections for 
} transmission electron microscopy. If I take the exact same tissue, a 
} piece of skin in this case, fix it in the same fixative, usually a 
} recipe containing buffered 2% para / 0.025% glut, for the same 
} fixation time, cryoprotect in sucrose for LM and PVP/sucrose for 
} TEM, then freeze and section 6 micron frozen sections for LM and 
} 100nm thick cryothin sections picked up on nickel formvar/carbon 
} grids for TEM, I get a completely different labeling pattern with 
} perhaps 10-20% of antibodies. Has anyone had similar observations? 
} If so, have you figured out why? I have tried to rule out PVP 
} (polyvinylpyrrolidone) as one of the obvious differences between the 
} two protocols but tissue cryoprotected with PVP/sucrose cannot be 
} cut at 6 microns in a cryostat. I have tried to
} rule out all other differences and remain stumped. As example, 
} labeling for a particular transcription factor shows 
} compartmentalized staining in the nucleus at the LM level and at the 
} TEM level specifically stains intercellular desmosomal adhesions 
} between the keratinocytes of the epidermis. Crazy!
}
} Sincerely,
} Robert Underwood
} University of Washington
} Dermatology
} Seattle, WA
}
}
}
}
}
}
}
} ==============================Original 
} Headers==============================
} 9, 22 -- From underwoo-at-u.washington.edu Fri Jun 12 11:11:51 2009
} 9, 22 -- Received: from mxout3.cac.washington.edu 
} (mxout3.cac.washington.edu [140.142.32.166])
} 9, 22 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP 
} id n5CGBmnS018118
} 9, 22 --     for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 11:11:50 
} -0500
} 9, 22 -- Received: from hymn13.u.washington.edu 
} (hymn13.u.washington.edu [140.142.4.103])
} 9, 22 --     by mxout3.cac.washington.edu 
} (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5CGBj0l020228
} 9, 22 --     (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 
} verify=NO)
} 9, 22 --     for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:47 
} -0700
} 9, 22 -- Received: from localhost (localhost [127.0.0.1])
} 9, 22 --     by hymn13.u.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) 
} with ESMTP id n5CGBgSq019694
} 9, 22 --     for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009 09:11:42 
} -0700
} 9, 22 -- X-Auth-Received: from [128.208.106.143] by 
} hymn13.u.washington.edu via HTTP; Fri, 12 Jun 2009 09:11:42 PDT
} 9, 22 -- Date: Fri, 12 Jun 2009 09:11:42 -0700 (PDT)
} 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
} 9, 22 -- To: Microscopy List {Microscopy-at-microscopy.Com}
} 9, 22 -- Subject: [Microscopy]:LM vs TEM immunostain patterns
} 9, 22 -- Message-ID: {Pine.LNX.4.43.0906120911420.10631-at-hymn13.u.washington.edu
} }
} 9, 22 -- MIME-Version: 1.0
} 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} 9, 22 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, 
} Antispam-Data: 2009.6.12.160138
} 9, 22 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
} 9, 22 --  SUPERLONG_LINE 0.05, BODY_SIZE_1300_1399 0, 
} BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, 
} __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, 
} __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, 
} __TO_MALFORMED_2 0'
} ==============================End of - 
} Headers==============================


==============================Original Headers==============================
12, 20 -- From leunissen-at-aurion.nl Fri Jun 12 15:06:32 2009
12, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
12, 20 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5CK6Uk4012793
12, 20 --     for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009 15:06:30 -0500
12, 20 -- Received: from [192.168.1.50] (really [122.57.248.181]) by fep02.xtra.co.nz
12, 20 --          with ESMTP
12, 20 --          id {20090612200628.PHMS28374.fep02.xtra.co.nz-at-[192.168.1.50]} ;
12, 20 --          Sat, 13 Jun 2009 08:06:28 +1200
12, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
12, 20 -- To: underwoo-at-u.washington.edu
12, 20 -- In-Reply-To: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
12, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
12, 20 -- References: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
12, 20 -- Message-Id: {490EE65A-A1CA-4DE4-B767-912DE666A018-at-aurion.nl}
12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 20 -- Content-Transfer-Encoding: 7bit
12, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
12, 20 -- Date: Sat, 13 Jun 2009 08:06:25 +1200
12, 20 -- Cc: microscopy-at-microscopy.com
12, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================






==============================Original Headers==============================
30, 25 -- From nizets2-at-yahoo.com Tue Jun 16 04:03:48 2009
30, 25 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231])
30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5G93lRB029336
30, 25 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 04:03:47 -0500
30, 25 -- Received: (qmail 75798 invoked by uid 60001); 16 Jun 2009 09:03:47 -0000
30, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245143026; bh=MEFlES/hYtB7mhvbYuj6ygxpqZXqXHDArEbpwXTHeYI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=budCPYcT83GtgbyG34Q3UDlKr7odRYBw84RfNztG8XsV9Dy/jrzLxIb0NA/0EZ7Yi1+fFIXSGfJ7glyjosifZLg0UpmBxjiW7NfFAfX7iBGdIHQgc4QFO6R3hEjYvKd6CXBJTvWdv/okGyDrxMA7Ew1dSpyNPpyEcv2Oy0/DdoI=
30, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
30, 25 -- s=s1024; d=yahoo.com;
30, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
30, 25 -- b=llK3EwHbI/8hg9yGEjsoFrlJFY9DUe96GZgFFF3aEBT/ZwoIeEffb+erJed8IctMPkmqd6klXIMbza/otpCZ/p+KvfWWTHjhO3ziYBiHrRBlZxFHv4RVnkYvUCNArvYY/X0nJcT3GrULhchsTjRvY49CtZtzYEaVQhSxb1dkUDA=;
30, 25 -- Message-ID: {884995.75697.qm-at-web110808.mail.gq1.yahoo.com}
30, 25 -- X-YMail-OSG: XEyC1J8VM1kxxv6VvG6MuIT_6NnlHC1m1QTWVg2ZX9.6TJJeEbUfyeiKZO96Vs74hMqLFqfymNqUbVx2k52063lTsu6fGhSlM3Id8Xu71tGWIjvfPJuCrsqnTzvdd2yUL_45NHFZoVBb.XrhhRpJcS.ZlddL3i4koUcFG_OyBOA_RKFEduH6b8SHz1TCB1OllrJCBIg3fjXrEkJtng4Du1ToHx_aS_eSwimzkFmmnJ6T6OpdBfpjsHLyXtkYJD4hzERCN4m.mSkJwc8JtSZCQgsVvd24JYrcazgLpVKTzqKY.LWOHTqUxIMXwJuKQKZ.v2HQComWseIN5NCxwCq2ayIiSSEyRfQjcPgjmsNyNr8-
30, 25 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Tue, 16 Jun 2009 02:03:46 PDT
30, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
30, 25 -- References: {200906122010.n5CKAMZv024835-at-ns.microscopy.com}
30, 25 -- Date: Tue, 16 Jun 2009 02:03:46 -0700 (PDT)
30, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
30, 25 -- Subject: Re: [Microscopy] Re: :LM vs TEM immunostain patterns
30, 25 -- To: underwoo-at-u.washington.edu
30, 25 -- Cc: microscopy-at-microscopy.com
30, 25 -- In-Reply-To: {200906122010.n5CKAMZv024835-at-ns.microscopy.com}
30, 25 -- MIME-Version: 1.0
30, 25 -- Content-Type: text/plain; charset=iso-8859-1
30, 25 -- Content-Transfer-Encoding: 8bit
30, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5G93lRB029336
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 16 Jun 2009 06:01:28 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't forget the lost cancer cure that Sean Connery was chasing in the
tropical forest of the Amazon. No electrical power, but he needed
compressed He for his GC. It was great GC too. Didn't need the MS and
gave complete structure of unknown compounds.

We must remember, the technology is just a plot device to add drama and
shore up sagging plots.

stay safe.........
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
5, 22 -- From frank_karl-at-lincolnelectric.com Tue Jun 16 06:01:27 2009
5, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GB1Rko017373
5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 06:01:27 -0500
5, 22 -- In-Reply-To: {200906160336.n5G3aGls002890-at-ns.microscopy.com}
5, 22 -- Subject: Re: [Microscopy] Re: Lab envy?
5, 22 -- To: Microscopy-at-microscopy.com
5, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
5, 22 -- Message-ID: {OF55E726DB.650ABBC1-ON852575D7.003BF45C-852575D7.003C82CF-at-lincolnelectric.com}
5, 22 -- Date: Tue, 16 Jun 2009 07:01:18 -0400
5, 22 -- From: Frank_Karl-at-lincolnelectric.com
5, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 22 -- 07, 2008) at 06/16/2009 07:01:15 AM,
5, 22 -- CD-MIME complete at 06/16/2009 07:01:15 AM,
5, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 22 -- 07, 2008) at 06/16/2009 07:01:15 AM,
5, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 22 -- 07, 2008) at 06/16/2009 07:01:15 AM,
5, 22 -- Serialize complete at 06/16/2009 07:01:15 AM
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain;
5, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Tue, 16 Jun 2009 07:07:32 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On the plus side -- did anyone see Ang Lee's "Incredible Hulk"? It
was roundly panned but the first third or so of the film features
many scenes in a molecular biology lab that felt amazingly right to
me (I didn't check to see if the pcr machines were upside down or
something). Anyone interested in the depiction of science in the
movies should check this one out.

TB

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
3, 20 -- From baskin-at-bio.umass.edu Tue Jun 16 07:07:32 2009
3, 20 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GC7WKx004263
3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 07:07:32 -0500
3, 20 -- Received: from [192.168.1.45] (ip-159.250.64.14.dsl.crocker.net [159.250.64.14])
3, 20 -- (authenticated bits=0)
3, 20 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n5GC7TwN015701
3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 08:07:31 -0400 (EDT)
3, 20 -- Mime-Version: 1.0
3, 20 -- Message-Id: {p0624050ac65d3a9f9e84-at-[192.168.1.45]}
3, 20 -- In-Reply-To: {200906161101.n5GB1uXV018156-at-ns.microscopy.com}
3, 20 -- References: {200906161101.n5GB1uXV018156-at-ns.microscopy.com}
3, 20 -- Date: Tue, 16 Jun 2009 08:07:27 -0400
3, 20 -- To: microscopy-at-microscopy.com
3, 20 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
3, 20 -- Subject: Re: [Microscopy] Lab envy?
3, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 20 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Tue, 16 Jun 2009 08:07:31 -0400 (EDT)
3, 20 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Tue, 16 Jun 2009 07:25:29 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My favourite is from an episode of the "The X Files". Scully is
investigating stories of an alien virus. The TEM operator shows Scully
an SEM image which looks like pollen or spores (boy! - those aliens are
cunning). Needless to say on driving home the TEM operator is involved
in a fatal car crash.

Dave

-----Original Message-----
X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu]
Sent: 16 June 2009 13:11
To: David Patton

On the plus side -- did anyone see Ang Lee's "Incredible Hulk"? It was
roundly panned but the first third or so of the film features many
scenes in a molecular biology lab that felt amazingly right to me (I
didn't check to see if the pcr machines were upside down or something).
Anyone interested in the depiction of science in the movies should check
this one out.

TB

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of
Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original
Headers==============================
3, 20 -- From baskin-at-bio.umass.edu Tue Jun 16 07:07:32 2009 3, 20 --
Received: from marlin.bio.umass.edu (marlin.bio.umass.edu
[128.119.55.19])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5GC7WKx004263
3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
07:07:32 -0500
3, 20 -- Received: from [192.168.1.45] (ip-159.250.64.14.dsl.crocker.net
[159.250.64.14])
3, 20 -- (authenticated bits=0)
3, 20 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id
n5GC7TwN015701
3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NO)
3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
08:07:31 -0400 (EDT)
3, 20 -- Mime-Version: 1.0
3, 20 -- Message-Id: {p0624050ac65d3a9f9e84-at-[192.168.1.45]}
3, 20 -- In-Reply-To: {200906161101.n5GB1uXV018156-at-ns.microscopy.com}
3, 20 -- References: {200906161101.n5GB1uXV018156-at-ns.microscopy.com}
3, 20 -- Date: Tue, 16 Jun 2009 08:07:27 -0400 3, 20 -- To:
microscopy-at-microscopy.com 3, 20 -- From: Tobias Baskin
{baskin-at-bio.umass.edu} 3, 20 -- Subject: Re: [Microscopy] Lab envy?
3, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 20 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by
milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Tue, 16 Jun
2009 08:07:31 -0400 (EDT) 3, 20 -- X-Scanned-By: MIMEDefang 2.54 on
128.119.55.19 ==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
15, 34 -- From David.Patton-at-uwe.ac.uk Tue Jun 16 07:25:29 2009
15, 34 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
15, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5GCPSDu019486
15, 34 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 07:25:28 -0500
15, 34 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
15, 34 -- id 77d5_c688d0da_5a70_11de_82fd_00142223915c;
15, 34 -- Tue, 16 Jun 2009 13:25:27 +0100
15, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
15, 34 -- (egen-uwe01.uwe.ac.uk [164.11.249.121])
15, 34 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
15, 34 -- 2005)) with ESMTP id {0KLB000GPZUF2U-at-mta01.uwe.ac.uk} for
15, 34 -- microscopy-at-microscopy.com; Tue, 16 Jun 2009 13:25:28 +0100 (BST)
15, 34 -- Date: Tue, 16 Jun 2009 13:24:08 +0100
15, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk}
15, 34 -- Subject: RE: [Microscopy] Re: Lab envy?
15, 34 -- In-reply-to: {200906161211.n5GCBDPD010145-at-ns.microscopy.com}
15, 34 -- To: baskin-at-bio.umass.edu
15, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB04D885D6-at-egen-uwe01}
15, 34 -- MIME-version: 1.0
15, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
15, 34 -- Content-type: text/plain; charset=us-ascii
15, 34 -- Content-class: urn:content-classes:message
15, 34 -- Thread-topic: [Microscopy] Re: Lab envy?
15, 34 -- Thread-index: Acnue5GE2LCgSFhvRV6/di5p5rQvSgAAQ1mQ
15, 34 -- X-MS-Has-Attach:
15, 34 -- X-MS-TNEF-Correlator:
15, 34 -- References: {200906161211.n5GCBDPD010145-at-ns.microscopy.com}
15, 34 -- X-NAIMIME-Disclaimer: 1
15, 34 -- X-NAIMIME-Modified: 1
15, 34 -- X-NAI-Spam-Score: 0
15, 34 -- X-NAI-Spam-Rules: 1 Rules triggered
15, 34 -- RV3297=0
15, 34 -- Content-Transfer-Encoding: 8bit
15, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GCPSDu019486
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 16 Jun 2009 07:31:14 -0500
Subject: [Microscopy] Re: Critical point drying and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

3 thoughts:
1) How is are the samples transported to your lab? In a sealed
dessicator with good dessicant? If not, they could be picking up
moisture from the air (Missouri ain't exactly dry).
2) Is this a brand new unit? There may be a thin film of oil on it
from manufacturing. Also, check that the o-rings sealing the chamber
are completely clean.
3) What supplier do they use for the CO2? Same as you? What grade CO2
did they get? Siphon tank, yes? Food grade CO2 is generally better.
If they used a different supplier, have them try yours, if they used
the same, they may just have gotten a bad cylinder. CO2 cylinders
will sometimes has oil and crud in them (and water) -- food-grade
cylinders have tighter controls on them for such things. They should
have both a no-go (water) filter/dessicator cartridge and a membrane
filter in the CO2 line.

I wouldn't blame charging on water left behind, that generally just
causes collapse and horrible specimens (how do they look otherwise?
OK?). Oil from the chamber or CO2 tank would cause charging.

Phil

} Dear Collective,
}
} As my coworker, tech extraordinaire Cheryl, just said, "This one is
} totally wacko!"
}
} One of our steady clients has acquired a new critical-point-dryer,
} identical to ours, and they are now having charging problems on their
} specimens. When they CPD in our unit, they have no charging. They have
} even CPD'd one sample in theirs, one in ours, mounted them on the same
} stub, coated them over here with our carbon evaporator, and viewed them
} together in our FESEM. The one from their CPD charges badly, the one
} from ours does not.
}
} All aspects of specimen prep were the same. The liquid CO2 and
} dehydration ethanol come from the same source, cover slips used for
} mounting are identical, etc., etc., etc. The only difference is that
} one specimen is CPD'd there and carried here and the other is CPD'd here
} and only carried to the coater and scope.
}
} Any thoughts? I can't wait to see the comments on this one!
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
5, 26 -- From oshel1pe-at-cmich.edu Tue Jun 16 07:31:14 2009
5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GCVD5R030760
5, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 07:31:14 -0500
5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n5GCVDYP005239;
5, 26 -- Tue, 16 Jun 2009 08:31:13 -0400
5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 26 -- Tue, 16 Jun 2009 08:31:13 -0400
5, 26 -- Mime-Version: 1.0
5, 26 -- Message-Id: {f06240800c65d3f14aa3f-at-[141.209.160.249]}
5, 26 -- In-Reply-To: {200906152109.n5FL9cQY023345-at-ns.microscopy.com}
5, 26 -- References: {200906152109.n5FL9cQY023345-at-ns.microscopy.com}
5, 26 -- Date: Tue, 16 Jun 2009 08:31:09 -0400
5, 26 -- To: TindallR-at-missouri.edu
5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 26 -- Subject: Re: [Microscopy] Critical point drying and charging
5, 26 -- Cc: Microscopy-at-microscopy.com
5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 26 -- X-OriginalArrivalTime: 16 Jun 2009 12:31:13.0680 (UTC) FILETIME=[563B8100:01C9EE7E]
5, 26 -- X-Canit-CHI2: 0.00
5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 26 -- X-Spam-Score: -4.20 () [Tag at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
5, 26 -- X-CanItPRO-Stream: default
5, 26 -- X-Canit-Stats-ID: 14432390 - 82d9e145750a
5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 16 Jun 2009 07:43:28 -0500
Subject: [Microscopy] Re: viaWWW: Coloring in Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peggy,

Microscopy Today, November/December 2003, Jerry Sedgewick, "Creating
Pseudocolored Images in Photoshop". pp 22-24.

Phil

} Email: mbisher-at-princeton.edu
} Name: Margaret Bisher
}
} Organization: Princeton University
}
} Title-Subject: [Filtered] Coloring in Photoshop
}
} Question: I would like to attempt to color two different types of
} cells in an SEM image. I recall an article, somewhere, (Microscopy
} Today or maybe Microscopy & Microanalysis?) about just this. I have
} started to look at my saved magazines, but I have not yet come across
} it. Can anyone help me out with this article, if they recall just
} where it was. If I remember, it was pretty helpful, going step by
} step on how to do it in Photoshop.
}
} Thank you! Peggy
}
} Margaret E. Bisher
} Electron Microscopy & Histology Core Facility Manager
} Department of Molecular Biology
} Princeton University
} Moffett Laboratory, Room 113
} Princeton, New Jersey
} Office: (609) 258-7026
} Fax: (609) 258-8468
} mbisher-at-princeton.edu
--
Philip Oshel
Technical Editor, Microscopy Today
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 26 -- From oshel1pe-at-cmich.edu Tue Jun 16 07:43:27 2009
4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GChRl4016022
4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 07:43:27 -0500
4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n5GCgmXN010856;
4, 26 -- Tue, 16 Jun 2009 08:43:26 -0400
4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 26 -- Tue, 16 Jun 2009 08:43:27 -0400
4, 26 -- Mime-Version: 1.0
4, 26 -- Message-Id: {f06240802c65d4357a9de-at-[141.209.160.249]}
4, 26 -- In-Reply-To: {200906152354.n5FNs48p007134-at-ns.microscopy.com}
4, 26 -- References: {200906152354.n5FNs48p007134-at-ns.microscopy.com}
4, 26 -- Date: Tue, 16 Jun 2009 08:43:25 -0400
4, 26 -- To: mbisher-at-princeton.edu
4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 26 -- Subject: Re: [Microscopy] viaWWW: Coloring in Photoshop
4, 26 -- Cc: Microscopy-at-microscopy.com
4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 26 -- X-OriginalArrivalTime: 16 Jun 2009 12:43:27.0339 (UTC) FILETIME=[0B86FBB0:01C9EE80]
4, 26 -- X-Canit-CHI2: 0.00
4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 26 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 26 -- X-CanItPRO-Stream: default
4, 26 -- X-Canit-Stats-ID: 14433813 - 4c54af549afc
4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 16 Jun 2009 08:32:58 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

And who can forget the SEM in the noodle shop in the street in the
immortal "Bladerunner". One stop shopping! Get a bowl of authentic
street noodles while getting a snake scale analyzed from an robot exotic
dancer's robot python (boa?).

You just can't make this stuff up. Oh, wait.....I guess they did,
didn't they....

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com





-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Monday, June 15, 2009 12:41 PM
To: Tindall, Randy D.

I was just watching an episode of NCIS (In infinite re-runs on USA
now...) and it dawned on me that they were using a mass spectrometer
which looked real to me- (Other things were off on their portrayal,
like timing, no specimen prep, and the results display...) and I was
just wondering if anyone out there has suffered "Lab envy" by watching
a TV show and thinking that that particular {insert lab equipment
here} would be put to much better use in your own lab...

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original
Headers==============================
3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
3, 31 -- Received: from mail-fx0-f212.google.com
(mail-fx0-f212.google.com [209.85.220.212])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5FHeTW9026891
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
12:40:29 -0500
3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
10:40:27 -0700 (PDT)
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 31 -- d=gmail.com; s=gamma;
3, 31 --
h=domainkey-signature:mime-version:received:date:message-id:subject
3, 31 -- :from:to:content-type:content-transfer-encoding;
3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
3, 31 --
b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
3, 31 --
Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 31 -- d=gmail.com; s=gamma;
3, 31 --
h=mime-version:date:message-id:subject:from:to:content-type
3, 31 -- :content-transfer-encoding;
3, 31 --
b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
3, 31 --
Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
3, 31 -- MIME-Version: 1.0
3, 31 -- Received: by 10.204.53.136 with SMTP id
m8mr7247200bkg.109.1245087627116; Mon,
3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
3, 31 -- Message-ID:
{25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
3, 31 -- Subject: Lab envy?
3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
3, 31 -- To: microscopy-at-microscopy.com
3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
3, 31 -- Content-Transfer-Encoding: 7bit
==============================End of -
Headers==============================



==============================Original Headers==============================
18, 29 -- From TindallR-at-missouri.edu Tue Jun 16 08:32:58 2009
18, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GDWvUj002293
18, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 08:32:57 -0500
18, 29 -- X-IronPort-Anti-Spam-Filtered: true
18, 29 -- X-IronPort-Anti-Spam-Result: ApoEAHM7N0rRauUo/2dsb2JhbADFFgEJhi2IUYJXgTYF
18, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
18, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Jun 2009 08:32:56 -0500
18, 29 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
18, 29 -- Tue, 16 Jun 2009 08:32:56 -0500
18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
18, 29 -- Content-class: urn:content-classes:message
18, 29 -- MIME-Version: 1.0
18, 29 -- Content-Type: text/plain;
18, 29 -- charset="us-ascii"
18, 29 -- Subject: RE: [Microscopy] Lab envy?
18, 29 -- Date: Tue, 16 Jun 2009 08:32:56 -0500
18, 29 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD81A0-at-UM-XMAIL08.um.umsystem.edu}
18, 29 -- In-Reply-To: {200906151741.n5FHfKZ8029105-at-ns.microscopy.com}
18, 29 -- X-MS-Has-Attach:
18, 29 -- X-MS-TNEF-Correlator:
18, 29 -- Thread-Topic: [Microscopy] Lab envy?
18, 29 -- Thread-Index: Acnt4ICPu8Io3DKJSr6FCJKOkCoskgApdI4g
18, 29 -- References: {200906151741.n5FHfKZ8029105-at-ns.microscopy.com}
18, 29 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
18, 29 -- To: {microscopy-at-microscopy.com}
18, 29 -- X-OriginalArrivalTime: 16 Jun 2009 13:32:56.0585 (UTC) FILETIME=[F555F790:01C9EE86]
18, 29 -- Content-Transfer-Encoding: 8bit
18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GDWvUj002293
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 16 Jun 2009 08:54:39 -0500
Subject: [Microscopy] CPD and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone for all the replies so far. I'm leaning toward the
residual oils explanation, but I don't recall this happening when we got
our CPD.

So, we're going to try two things initially: 1) We're bringing both
CPDs to Tina's lab for side-by-side testing until we get to the bottom
of this, and 2) where do you buy rubber chickens?

Keep 'em coming!

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
8, 27 -- From TindallR-at-missouri.edu Tue Jun 16 08:54:39 2009
8, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GDscsf018865
8, 27 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 08:54:38 -0500
8, 27 -- X-IronPort-Anti-Spam-Filtered: true
8, 27 -- X-IronPort-Anti-Spam-Result: ApoEAE9BN0rRauUo/2dsb2JhbADFQQEJhiyIUYJXgTYF
8, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
8, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Jun 2009 08:54:38 -0500
8, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 27 -- Tue, 16 Jun 2009 08:54:38 -0500
8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 27 -- Content-class: urn:content-classes:message
8, 27 -- MIME-Version: 1.0
8, 27 -- Content-Type: text/plain;
8, 27 -- charset="us-ascii"
8, 27 -- Subject: CPD and charging
8, 27 -- Date: Tue, 16 Jun 2009 08:54:38 -0500
8, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD81A1-at-UM-XMAIL08.um.umsystem.edu}
8, 27 -- X-MS-Has-Attach:
8, 27 -- X-MS-TNEF-Correlator:
8, 27 -- Thread-Topic: CPD and charging
8, 27 -- Thread-Index: AcnuifzpsutQ0hokR16KUaLUZ3mq8Q==
8, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
8, 27 -- To: {microscopy-at-microscopy.com}
8, 27 -- X-OriginalArrivalTime: 16 Jun 2009 13:54:38.0807 (UTC) FILETIME=[FD854670:01C9EE89]
8, 27 -- Content-Transfer-Encoding: 8bit
8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GDscsf018865
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 16 Jun 2009 09:02:20 -0500
Subject: [Microscopy] Re: CPD and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy!
From KFC of course.
(Kentucky Faux Chicken).
Phil

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
2, 25 -- From oshel1pe-at-cmich.edu Tue Jun 16 09:02:20 2009
2, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GE2JZv000993
2, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 09:02:20 -0500
2, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
2, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n5GE0cCI003345
2, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:02:16 -0400
2, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
2, 25 -- Tue, 16 Jun 2009 10:01:44 -0400
2, 25 -- Mime-Version: 1.0
2, 25 -- Message-Id: {f06240803c65d56130db8-at-[141.209.160.249]}
2, 25 -- In-Reply-To: {200906161357.n5GDvkxO024794-at-ns.microscopy.com}
2, 25 -- References: {200906161357.n5GDvkxO024794-at-ns.microscopy.com}
2, 25 -- Date: Tue, 16 Jun 2009 10:01:43 -0400
2, 25 -- To: Microscopy-at-microscopy.com
2, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
2, 25 -- Subject: Re: [Microscopy] CPD and charging
2, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
2, 25 -- X-OriginalArrivalTime: 16 Jun 2009 14:01:44.0408 (UTC) FILETIME=[FB32D580:01C9EE8A]
2, 25 -- X-Canit-CHI2: 0.00
2, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
2, 25 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
2, 25 -- X-CanItPRO-Stream: default
2, 25 -- X-Canit-Stats-ID: 14438897 - 0d4201a82728
2, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Tue, 16 Jun 2009 10:00:14 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peggy,
I think the article you are remembering is "Creating Pseudocolored Images in
Photoshop". You can find it in the November 2003 issue of Microscopy Today.
I tried it and it works!
Good Luck,
Pat Kysar
University of California, Davis
Medical School, Pathology
EM Lab

----- Original Message -----
X-from: {mbisher-at-princeton.edu}
To: {pekysar-at-ucdavis.edu}
Sent: Monday, June 15, 2009 4:58 PM

Speaking of instruments in movies, what is the earliest appearance of
an SEM in any movie? I've always thought that "The Man in the White
Suit" deserved that honor (anyone know which model it was?), but
maybe there is an earlier debut?

If you haven't seen Alec Guinness in the above movie you're in for a
treat- very entertaining.

On a related note, can anyone recall an electron microprobe in any movie?
john

John J. Donovan
Director, MicroAnalytical Facility
CAMCOR, University of Oregon

(541) 346-4632 (office)
(541) 346-4655 (lab)

www.camcor.uoregon.edu
www.epmalab.uoregon.edu




==============================Original Headers==============================
9, 20 -- From donovan-at-uoregon.edu Tue Jun 16 10:00:13 2009
9, 20 -- Received: from QMTA05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GF0D0N032662
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:00:13 -0500
9, 20 -- Message-Id: {200906161500.n5GF0D0N032662-at-ns.microscopy.com}
9, 20 -- Received: from OMTA14.emeryville.ca.mail.comcast.net ([76.96.30.60])
9, 20 -- by QMTA05.emeryville.ca.mail.comcast.net with comcast
9, 20 -- id 4bbJ1c0041HpZEsA5f0EkA; Tue, 16 Jun 2009 15:00:14 +0000
9, 20 -- Received: from SOURCE.uoregon.edu ([71.237.220.159])
9, 20 -- by OMTA14.emeryville.ca.mail.comcast.net with comcast
9, 20 -- id 4f0D1c00E3SwvZT8af0DB3; Tue, 16 Jun 2009 15:00:13 +0000
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Tue, 16 Jun 2009 08:00:12 -0700
9, 20 -- To: microscopy-at-microscopy.com
9, 20 -- From: "John J. Donovan" {donovan-at-uoregon.edu}
9, 20 -- Subject: Re: [Microscopy] Re: Lab envy?
9, 20 -- In-Reply-To: {200906161212.n5GCC7i5011972-at-ns.microscopy.com}
9, 20 -- References: {200906161212.n5GCC7i5011972-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Tue, 16 Jun 2009 10:02:08 -0500
Subject: [Microscopy] EM in film/TV (was: Lab envy?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

TindallR-at-missouri.edu wrote:
}
} And who can forget the SEM in the noodle shop in the street in the
} immortal "Bladerunner". One stop shopping! Get a bowl of authentic
} street noodles while getting a snake scale analyzed from an robot exotic
} dancer's robot python (boa?).
}
It's been a dozen years since I mentioned this, so maybe
there are some new folks who don't know it.

Star Trek V used an SEM for one of its special effects.
The planet beyond the end of the universe was an SEM
image of a crystalline mineral at low mag. They bought
a Zeiss SEM and a PGT imaging system specifically for
the purpose. A film industry motion-control software
expert was brought it to "fly" the field of view through
the crystals at a high tilt angle.

The digital image resolution wasn't all that great, so the
frames were blurred with blue fog in post-processing.
The end result may not have been all that wonderful
by comparison with modern all-digital animation, but
the idea was pretty cool. The long in-focus depth of
field was supposed to make it look otherworldly.

Rick Mott, PulseTor LLC (then at PGT, and visited
the special-effects house for this project)


==============================Original Headers==============================
5, 18 -- From rmott-at-pulsetor.com Tue Jun 16 10:02:08 2009
5, 18 -- Received: from smtpauth21.prod.mesa1.secureserver.net (smtpauth21.prod.mesa1.secureserver.net [64.202.165.38])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5GF27ss003556
5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:02:08 -0500
5, 18 -- Received: (qmail 18425 invoked from network); 16 Jun 2009 15:02:02 -0000
5, 18 -- Received: from unknown (98.221.245.7)
5, 18 -- by smtpauth21.prod.mesa1.secureserver.net (64.202.165.38) with ESMTP; 16 Jun 2009 15:01:58 -0000
5, 18 -- Message-ID: {4A37B3EA.6070509-at-pulsetor.com}
5, 18 -- Date: Tue, 16 Jun 2009 11:02:02 -0400
5, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
5, 18 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: microscopy-at-microscopy.com
5, 18 -- Subject: Re: [Microscopy] RE: EM in film/TV (was: Lab envy?)
5, 18 -- References: {200906161340.n5GDelaE013811-at-ns.microscopy.com}
5, 18 -- In-Reply-To: {200906161340.n5GDelaE013811-at-ns.microscopy.com}
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Tue, 16 Jun 2009 10:02:44 -0500
Subject: [Microscopy] EM in film/TV (was: Lab envy?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

TindallR-at-missouri.edu wrote:
}
} And who can forget the SEM in the noodle shop in the street in the
} immortal "Bladerunner". One stop shopping! Get a bowl of authentic
} street noodles while getting a snake scale analyzed from an robot exotic
} dancer's robot python (boa?).
}
It's been a dozen years since I mentioned this, so maybe
there are some new folks who don't know it.

Star Trek V used an SEM for one of its special effects.
The planet beyond the end of the universe was an SEM
image of a crystalline mineral at low mag. They bought
a Zeiss SEM and a PGT imaging system specifically for
the purpose. A film industry motion-control software
expert was brought it to "fly" the field of view through
the crystals at a high tilt angle.

The digital image resolution wasn't all that great, so the
frames were blurred with blue fog in post-processing.
The end result may not have been all that wonderful
by comparison with modern all-digital animation, but
the idea was pretty cool. The long in-focus depth of
field was supposed to make it look otherworldly.

Rick Mott, PulseTor LLC (then at PGT, and visited
the special-effects house for this project)


==============================Original Headers==============================
5, 18 -- From rmott-at-pulsetor.com Tue Jun 16 10:02:43 2009
5, 18 -- Received: from smtpout09.prod.mesa1.secureserver.net (smtpout09-01.prod.mesa1.secureserver.net [64.202.165.14])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5GF2hY7005063
5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:02:43 -0500
5, 18 -- Received: (qmail 13925 invoked from network); 16 Jun 2009 15:02:43 -0000
5, 18 -- Received: from unknown (98.221.245.7)
5, 18 -- by smtpout09.prod.mesa1.secureserver.net (64.202.165.14) with ESMTP; 16 Jun 2009 15:02:42 -0000
5, 18 -- Message-ID: {4A37B416.2090203-at-pulsetor.com}
5, 18 -- Date: Tue, 16 Jun 2009 11:02:46 -0400
5, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
5, 18 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: microscopy-at-microscopy.com
5, 18 -- Subject: Re: [Microscopy] RE: EM in film/TV (was: Lab envy?)
5, 18 -- References: {200906161340.n5GDelaE013811-at-ns.microscopy.com}
5, 18 -- In-Reply-To: {200906161340.n5GDelaE013811-at-ns.microscopy.com}
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Tue, 16 Jun 2009 10:17:13 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I worked with a microscope rep who had consulted on two episodes of CSI Miami. He said that it was fun, but a lot of flying and standing around considering he was working for nothing but perks. He also mentioned that some actors/actresses will pay close attention to his instruction and try to be as accurate as possible. Others will do whatever they feel like, no matter how long you do demos and instruction for them.

I'm pretty sure he said the equipment was loaned.

Regards,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Monday, June 15, 2009 1:49 PM
To: Sobocinski, Gregg

I was just watching an episode of NCIS (In infinite re-runs on USA
now...) and it dawned on me that they were using a mass spectrometer
which looked real to me- (Other things were off on their portrayal,
like timing, no specimen prep, and the results display...) and I was
just wondering if anyone out there has suffered "Lab envy" by watching
a TV show and thinking that that particular {insert lab equipment
here} would be put to much better use in your own lab...

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
3, 31 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5FHeTW9026891
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 12:40:29 -0500
3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009 10:40:27 -0700 (PDT)
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
3, 31 -- :from:to:content-type:content-transfer-encoding;
3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
3, 31 -- b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
3, 31 -- Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 31 -- d=gmail.com; s=gamma;
3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
3, 31 -- :content-transfer-encoding;
3, 31 -- b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
3, 31 -- Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
3, 31 -- MIME-Version: 1.0
3, 31 -- Received: by 10.204.53.136 with SMTP id m8mr7247200bkg.109.1245087627116; Mon,
3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
3, 31 -- Message-ID: {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
3, 31 -- Subject: Lab envy?
3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
3, 31 -- To: microscopy-at-microscopy.com
3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
3, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================


==============================Original Headers==============================
13, 25 -- From greggps-at-umich.edu Tue Jun 16 10:17:12 2009
13, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GFHCqv010610
13, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:17:12 -0500
13, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.235]) by
13, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Tue, 16 Jun
13, 25 -- 2009 11:17:12 -0400
13, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
13, 25 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
13, 25 -- Date: Tue, 16 Jun 2009 11:17:19 -0400
13, 25 -- Subject: RE: [Microscopy] Lab envy? Equipment usage in Film Industry
13, 25 -- Thread-Topic: [Microscopy] Lab envy? Equipment usage in Film Industry
13, 25 -- Thread-Index: Acnt4YU6HKA01FRFTqutkcGq6ns2QQAsyLkA
13, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA393406156C2E25-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
13, 25 -- References: {200906151748.n5FHmV8A011810-at-ns.microscopy.com}
13, 25 -- In-Reply-To: {200906151748.n5FHmV8A011810-at-ns.microscopy.com}
13, 25 -- Accept-Language: en-US
13, 25 -- Content-Language: en-US
13, 25 -- X-MS-Has-Attach:
13, 25 -- X-MS-TNEF-Correlator:
13, 25 -- acceptlanguage: en-US
13, 25 -- Content-Type: text/plain; charset="us-ascii"
13, 25 -- MIME-Version: 1.0
13, 25 -- Content-Transfer-Encoding: 8bit
13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GFHCqv010610
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Tue, 16 Jun 2009 10:27:26 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe the "electron microscope" in "The Man in the White Suit" in
a TEM as seen here:

http://www.tcm.com/mediaroom/index.jsp?cid=222698

Someone here should be able to identify the manufacturer and model.

Unfortunately I've never seen an electron microprobe in a film -- let
me know if anyone ever finds one!

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010





==============================Original Headers==============================
10, 20 -- From frah0010-at-umn.edu Tue Jun 16 10:27:26 2009
10, 20 -- Received: from mta-m3.tc.umn.edu (mta-m3.tc.umn.edu [134.84.135.123])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GFRQG9025072
10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:27:26 -0500
10, 20 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133])
10, 20 -- by mta-m3.tc.umn.edu (UMN smtpd) with ESMTP
10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:27:25 -0500 (CDT)
10, 20 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU
10, 20 -- X-Umn-Classification: local
10, 20 -- Message-Id: {6EAF6A1E-0672-45DA-B4A5-0B8CC5FED48B-at-umn.edu}
10, 20 -- From: Ellery Frahm {frah0010-at-umn.edu}
10, 20 -- To: microscopy-at-microscopy.com
10, 20 -- In-Reply-To: {200906161506.n5GF6LbO018543-at-ns.microscopy.com}
10, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 20 -- Content-Transfer-Encoding: 7bit
10, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
10, 20 -- Subject: Re: [Microscopy] Lab envy?
10, 20 -- Date: Tue, 16 Jun 2009 10:27:25 -0500
10, 20 -- References: {200906161506.n5GF6LbO018543-at-ns.microscopy.com}
10, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Tue, 16 Jun 2009 10:36:14 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes, of course, Ellery is correct- I meant to say first electron microscope.

Ok, so what's the earliest SEM in a movie though? Must be some 50's
sci-fi picture that had one.
john

At 08:31 AM 6/16/2009, you wrote:

} I believe the "electron microscope" in "The Man in the White Suit" in
} a TEM as seen here:
}
} http://www.tcm.com/mediaroom/index.jsp?cid=222698
}
} Someone here should be able to identify the manufacturer and model.
}
} Unfortunately I've never seen an electron microprobe in a film -- let
} me know if anyone ever finds one!
}
} Best,
} Ellery
}
} -----------------
} Ellery Frahm
} Senior Research Fellow, Department of Geology & Geophysics
} Manager & Principal Analyst, Electron Microprobe Lab
} Doctoral Candidate, Department of Anthropology
} University of Minnesota - Twin Cities campus
} http://umn.edu/~frah0010


==============================Original Headers==============================
5, 20 -- From donovan-at-uoregon.edu Tue Jun 16 10:36:13 2009
5, 20 -- Received: from QMTA03.emeryville.ca.mail.comcast.net (qmta03.emeryville.ca.mail.comcast.net [76.96.30.32])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GFaBEs007062
5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:36:12 -0500
5, 20 -- Message-Id: {200906161536.n5GFaBEs007062-at-ns.microscopy.com}
5, 20 -- Received: from OMTA11.emeryville.ca.mail.comcast.net ([76.96.30.36])
5, 20 -- by QMTA03.emeryville.ca.mail.comcast.net with comcast
5, 20 -- id 4bDB1c0020mlR8UA3fcB7F; Tue, 16 Jun 2009 15:36:11 +0000
5, 20 -- Received: from SOURCE.uoregon.edu ([71.237.220.159])
5, 20 -- by OMTA11.emeryville.ca.mail.comcast.net with comcast
5, 20 -- id 4fc91c00V3SwvZT8XfcAUj; Tue, 16 Jun 2009 15:36:10 +0000
5, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
5, 20 -- Date: Tue, 16 Jun 2009 08:36:08 -0700
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- From: "John J. Donovan" {donovan-at-uoregon.edu}
5, 20 -- Subject: Re: [Microscopy] Re: Lab envy?
5, 20 -- In-Reply-To: {200906161531.n5GFV4Rl032539-at-ns.microscopy.com}
5, 20 -- References: {200906161531.n5GFV4Rl032539-at-ns.microscopy.com}
5, 20 -- Mime-Version: 1.0
5, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 16 Jun 2009 10:46:29 -0500
Subject: [Microscopy] Fw: EM in film/TV

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Opps......... too fast on the send button
----- Forwarded by Frank Karl/Lincoln Electric/US on 06/16/2009 11:45 AM
-----

Frank
Karl/Lincoln
Electric/US To
rmott-at-pulsetor.com
06/16/2009 11:45 cc
AM
Subject
EM in film/TV (Document link:
Frank Karl)









Well since we're heading this way and making such good time...

Try: Buckaroo Bonzi: Adventure in the 8th Dimension. The 8th dimension
sports quite a few SEM images. Years ago in the advertisements for the
early Sci-Fi channel used different photomicrographs and SEM images in a
montage like effect. I seem to remember Brian Ford talking about those
images. Maybe he could elaborate............

stay safe......
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
9, 21 -- From frank_karl-at-lincolnelectric.com Tue Jun 16 10:46:29 2009
9, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GFkThv021835
9, 21 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:46:29 -0500
9, 21 -- Subject: Fw: EM in film/TV
9, 21 -- To: Microscopy-at-microscopy.com
9, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
9, 21 -- Message-ID: {OFFC41C4E1.664C6990-ON852575D7.00569485-852575D7.00569A60-at-lincolnelectric.com}
9, 21 -- Date: Tue, 16 Jun 2009 11:46:17 -0400
9, 21 -- From: Frank_Karl-at-lincolnelectric.com
9, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 06/16/2009 11:46:14 AM,
9, 21 -- CD-MIME complete at 06/16/2009 11:46:14 AM,
9, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 06/16/2009 11:46:14 AM,
9, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 06/16/2009 11:46:14 AM,
9, 21 -- Serialize complete at 06/16/2009 11:46:14 AM
9, 21 -- MIME-Version: 1.0
9, 21 -- Content-Type: text/plain;
9, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Tue, 16 Jun 2009 10:54:55 -0500
Subject: [Microscopy] Fwd: RE: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mmm... Bladerunner... hard to believe it came out of the same hands as
the [deleted] like Gladiator and Kingdom of Heaven...

Anyway - I can't believe noone has mentioned Spiderman? With that huge
TEM sitting in the middle of a bright hall in the museum?..

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
} Date: June 16, 2009 9:34:28 AM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] RE: Lab envy?
} Reply-To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} And who can forget the SEM in the noodle shop in the street in the
} immortal "Bladerunner". One stop shopping! Get a bowl of authentic
} street noodles while getting a snake scale analyzed from an robot
} exotic
} dancer's robot python (boa?).
}
} You just can't make this stuff up. Oh, wait.....I guess they did,
} didn't they....
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
}
} -----Original Message-----
} X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} Sent: Monday, June 15, 2009 12:41 PM
} To: Tindall, Randy D.
} Subject: [Microscopy] Lab envy?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} I was just watching an episode of NCIS (In infinite re-runs on USA
} now...) and it dawned on me that they were using a mass spectrometer
} which looked real to me- (Other things were off on their portrayal,
} like timing, no specimen prep, and the results display...) and I was
} just wondering if anyone out there has suffered "Lab envy" by watching
} a TV show and thinking that that particular {insert lab equipment
} here} would be put to much better use in your own lab...
}
} --Justin A. Kraft
}
} --
} "America believes in education; the average professor earns more money
} in a year than a professional athlete earns in a whole week." Evan
} Esar
}
} ==============================Original
} Headers==============================
} 3, 31 -- From kraftpiano-at-gmail.com Mon Jun 15 12:40:30 2009
} 3, 31 -- Received: from mail-fx0-f212.google.com
} (mail-fx0-f212.google.com [209.85.220.212])
} 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5FHeTW9026891
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 12:40:29 -0500
} 3, 31 -- Received: by fxm8 with SMTP id 8so4027818fxm.18
} 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2009
} 10:40:27 -0700 (PDT)
} 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 --
} h=domainkey-signature:mime-version:received:date:message-id:subject
} 3, 31 -- :from:to:content-type:content-transfer-encoding;
} 3, 31 -- bh=B2dF4hm5bWMXo7UdW987EMxVrlIUyAwH2M0NFYYmrgc=;
} 3, 31 --
} b=NDHfu42Eitt+TwedOQjoJsE0CKJlX/LNcw3Mm5DPcXxzmuqaKLw7wmDdbjfOtR2vhp
} 3, 31 --
} Yl1b9h94MXjB6kh6bDycgIdKL9C32BoNitrbUNVgUhuMtMEp+WJTwve++x7gfmX1Ofi6
} 3, 31 -- DXLVaI0556WW4a8dwVsq5VahlUtYezeuAECeM=
} 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 3, 31 -- d=gmail.com; s=gamma;
} 3, 31 --
} h=mime-version:date:message-id:subject:from:to:content-type
} 3, 31 -- :content-transfer-encoding;
} 3, 31 --
} b=NjxazExQkQXlnTkGHvSKCfZJXz5GuMtl8axCvOCk9UsjrdFe3HOGskCgM6TECJ0bxo
} 3, 31 --
} Qj1V87HoL5Fs9pECqFWm7ZvSAKoroF9VMpKPpzGlzI1CPFZOhQrJ1maIFu8I1rkbW/v9
} 3, 31 -- txl+MQyAN5dqt5rCpjbf+y8VbmLljbm1pu0mM=
} 3, 31 -- MIME-Version: 1.0
} 3, 31 -- Received: by 10.204.53.136 with SMTP id
} m8mr7247200bkg.109.1245087627116; Mon,
} 3, 31 -- 15 Jun 2009 10:40:27 -0700 (PDT)
} 3, 31 -- Date: Mon, 15 Jun 2009 13:40:27 -0400
} 3, 31 -- Message-ID:
} {25e2b0d20906151040t11a55beeo2c44ef8573eda6c8-at-mail.gmail.com}
} 3, 31 -- Subject: Lab envy?
} 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} 3, 31 -- To: microscopy-at-microscopy.com
} 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1
} 3, 31 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 18, 29 -- From TindallR-at-missouri.edu Tue Jun 16 08:32:58 2009
} 18, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
} (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n5GDWvUj002293
} 18, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
} 08:32:57 -0500
} 18, 29 -- X-IronPort-Anti-Spam-Filtered: true
} 18, 29 -- X-IronPort-Anti-Spam-Result: ApoEAHM7N0rRauUo/
} 2dsb2JhbADFFgEJhi2IUYJXgTYF
} 18, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 18, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Jun
} 2009 08:32:56 -0500
} 18, 29 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft
} SMTPSVC(6.0.3790.3959);
} 18, 29 -- Tue, 16 Jun 2009 08:32:56 -0500
} 18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 18, 29 -- Content-class: urn:content-classes:message
} 18, 29 -- MIME-Version: 1.0
} 18, 29 -- Content-Type: text/plain;
} 18, 29 -- charset="us-ascii"
} 18, 29 -- Subject: RE: [Microscopy] Lab envy?
} 18, 29 -- Date: Tue, 16 Jun 2009 08:32:56 -0500
} 18, 29 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD81A0-at-UM-XMAIL08.um.umsystem.edu
} }
} 18, 29 -- In-Reply-To: {200906151741.n5FHfKZ8029105-at-ns.microscopy.com}
} 18, 29 -- X-MS-Has-Attach:
} 18, 29 -- X-MS-TNEF-Correlator:
} 18, 29 -- Thread-Topic: [Microscopy] Lab envy?
} 18, 29 -- Thread-Index: Acnt4ICPu8Io3DKJSr6FCJKOkCoskgApdI4g
} 18, 29 -- References: {200906151741.n5FHfKZ8029105-at-ns.microscopy.com}
} 18, 29 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 18, 29 -- To: {microscopy-at-microscopy.com}
} 18, 29 -- X-OriginalArrivalTime: 16 Jun 2009 13:32:56.0585 (UTC)
} FILETIME=[F555F790:01C9EE86]
} 18, 29 -- Content-Transfer-Encoding: 8bit
} 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n5GDWvUj002293
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
7, 23 -- From vladislav_speransky-at-nih.gov Tue Jun 16 10:54:54 2009
7, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GFsqVV003656
7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 10:54:53 -0500
7, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
7, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id 5F19C361B80
7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 11:54:50 -0400 (EDT)
7, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
7, 23 -- by compute1.internal (MEProxy); Tue, 16 Jun 2009 11:54:50 -0400
7, 23 -- X-Sasl-enc: nphkr6EK31Ee/AIHMIa9OXufhwLIg8VLIZp/SazeH3Nu 1245167690
7, 23 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185])
7, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id 1CA8E179E2
7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 11:54:50 -0400 (EDT)
7, 23 -- Message-Id: {3F81DB17-693E-493F-897C-2FA7643D77EE-at-nih.gov}
7, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
7, 23 -- To: Microscopy-at-microscopy.com
7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 23 -- Content-Transfer-Encoding: 7bit
7, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3)
7, 23 -- Subject: Fwd: [Microscopy] RE: Lab envy?
7, 23 -- Date: Tue, 16 Jun 2009 11:54:48 -0400
7, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2563D2C7-at-NIHMLBX02.nih.gov}
7, 23 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: auntdaisy-at-gmail.com
Date: Tue, 16 Jun 2009 11:18:27 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Doctor Who has a SEM in the Tom Baker episode called "The Hand of Fear"
(http://www.drwhoguide.com/who_4n.htm) Part of a silicon-based life-form is
examined in it.

Austin Day

} } -----Original Message-----
} } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } Sent: Monday, June 15, 2009 12:41 PM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] Lab envy?
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } ----
} }
} } I was just watching an episode of NCIS (In infinite re-runs on USA
} } now...) and it dawned on me that they were using a mass spectrometer
} } which looked real to me- (Other things were off on their portrayal,
} } like timing, no specimen prep, and the results display...) and I was
} } just wondering if anyone out there has suffered "Lab envy" by watching
} } a TV show and thinking that that particular {insert lab equipment
} } here} would be put to much better use in your own lab...
} }
} } --Justin A. Kraft
} }
} } --
} } "America believes in education; the average professor earns more money
} } in a year than a professional athlete earns in a whole week." Evan
} } Esar


==============================Original Headers==============================
4, 45 -- From auntdaisy-at-gmail.com Tue Jun 16 11:18:27 2009
4, 45 -- Received: from mail-ew0-f211.google.com (mail-ew0-f211.google.com [209.85.219.211])
4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GGIQbR018877
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 11:18:27 -0500
4, 45 -- Received: by ewy7 with SMTP id 7so7719349ewy.18
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 09:18:26 -0700 (PDT)
4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 45 -- d=gmail.com; s=gamma;
4, 45 -- h=domainkey-signature:received:received:message-id:from:to:references
4, 45 -- :subject:date:mime-version:content-type:content-transfer-encoding
4, 45 -- :x-priority:x-msmail-priority:x-mailer:x-mimeole;
4, 45 -- bh=ml6/DOLceFlBhWNY+014aKBAmy3F+RjdcsIMMa1mCCw=;
4, 45 -- b=ee3He76vaAZYUsupO7onZC4ywrlybBNVjjZcXnkGj78z+YL4coM5pDVNGSrc/PzayR
4, 45 -- ghFM88ZN8qW2C69R/99OK4EIHZtT8OVL6e9aF1bt2N6fpFFs+hiRnwgBfNDlst2iPejW
4, 45 -- os9Yqv1ZtE7LNXY1bhWukc9ls2JAloXJ0abQU=
4, 45 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 45 -- d=gmail.com; s=gamma;
4, 45 -- h=message-id:from:to:references:subject:date:mime-version
4, 45 -- :content-type:content-transfer-encoding:x-priority:x-msmail-priority
4, 45 -- :x-mailer:x-mimeole;
4, 45 -- b=wXRmQ6PiVW016Wf/AvbvYloU21JUs8Z5TZAFuS1wClalft3h0eao0HLQIAWbI5dEuQ
4, 45 -- zPSmypBrL3FYeo5DeLpX6LJp/C01GyP6UByEGm5GqZF/WAWI5zIg5tiNsyJT0dm9xEOH
4, 45 -- z/i+RsUVlXnupQ2K2IB/UjkMlRLdHCtnLFrVw=
4, 45 -- Received: by 10.216.19.141 with SMTP id n13mr2919614wen.47.1245169105797;
4, 45 -- Tue, 16 Jun 2009 09:18:25 -0700 (PDT)
4, 45 -- Received: from AuntDaisy ([82.195.101.81])
4, 45 -- by mx.google.com with ESMTPS id 23sm50764eya.19.2009.06.16.09.18.24
4, 45 -- (version=SSLv3 cipher=RC4-MD5);
4, 45 -- Tue, 16 Jun 2009 09:18:25 -0700 (PDT)
4, 45 -- Message-ID: {C3F13D9303994F2E89F08BD67BEBA363-at-AuntDaisy}
4, 45 -- From: ADay {auntdaisy-at-gmail.com}
4, 45 -- To: {Microscopy-at-microscopy.com}
4, 45 -- References: {200906161557.n5GFvkAC008665-at-ns.microscopy.com}
4, 45 -- Subject: Re: [Microscopy] Lab envy?
4, 45 -- Date: Tue, 16 Jun 2009 17:18:07 +0100
4, 45 -- MIME-Version: 1.0
4, 45 -- Content-Type: text/plain;
4, 45 -- format=flowed;
4, 45 -- charset="iso-8859-1";
4, 45 -- reply-type=original
4, 45 -- Content-Transfer-Encoding: 7bit
4, 45 -- X-Priority: 3
4, 45 -- X-MSMail-Priority: Normal
4, 45 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138
4, 45 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Tue, 16 Jun 2009 11:21:16 -0500
Subject: [Microscopy] Re: Fwd: RE: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I actually kind of liked the EM shown in the first "Spiderman" film --
it worked in a comic book film. For those unfamiliar, here is an image:

http://screencapheaven.com/testcoppermine/displayimage.php?pid=3029&fullsize=1

It actually doesn't look too different in size and shape than JEOL's
ultra-high-voltage TEMs:

http://hints.es.hokudai.ac.jp/en/instruments/img/image21.jpg
http://www.mext.go.jp/english/org/struct/image/029.jpg
http://www.icb.osaka-u.ac.jp/images/clip_image002.jpg

The scientist leading the tour calls it "largest electron microscope
on the eastern seaboard." The problem, though, is that it appears the
cell images shown on the screens come from a phase contrast
microscope, not a TEM or SEM. Presumably that was just to show the
cells moving.

All in all, it wasn't too bad a representation of electron microscopy
for a Hollywood blockbuster.

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010


==============================Original Headers==============================
9, 20 -- From frah0010-at-umn.edu Tue Jun 16 11:21:16 2009
9, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GGLFkn021853
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 11:21:16 -0500
9, 20 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133])
9, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 11:21:14 -0500 (CDT)
9, 20 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU
9, 20 -- X-Umn-Classification: local
9, 20 -- Message-Id: {E9ACAB40-4B27-4C3F-BFA4-725927F53DDB-at-umn.edu}
9, 20 -- From: Ellery Frahm {frah0010-at-umn.edu}
9, 20 -- To: microscopy-at-microscopy.com
9, 20 -- In-Reply-To: {200906161559.n5GFx1la011281-at-ns.microscopy.com}
9, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 20 -- Content-Transfer-Encoding: 7bit
9, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
9, 20 -- Subject: Re: [Microscopy] Fwd: RE: Lab envy?
9, 20 -- Date: Tue, 16 Jun 2009 11:21:13 -0500
9, 20 -- References: {200906161559.n5GFx1la011281-at-ns.microscopy.com}
9, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Tue, 16 Jun 2009 12:29:09 -0500
Subject: [Microscopy] Re: Fwd: RE: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery -

Not to split hairs here, but the last two images of your JEOL TEMs say
'Hitachi' on their sides.

Also interesting is that the Spiderman EM lacks the walkways (though I
am not familiar with the workings of these enormous EMs, I assume they
are mostly for maintenance access?) and guard rails present on the
actual ones. I suppose the EM in the movie never requires maintenance,
and even if it does, there aren't hordes of lawyers running amok, making
guard rails and such necessary. Wouldn't that be nice?

David

-----Original Message-----
X-from: frah0010-at-umn.edu [mailto:frah0010-at-umn.edu]
Sent: Tuesday, June 16, 2009 12:30 PM
To: Parmiter, David (NIH/NCI) [C]

I actually kind of liked the EM shown in the first "Spiderman" film --
it worked in a comic book film. For those unfamiliar, here is an image:

http://screencapheaven.com/testcoppermine/displayimage.php?pid=3029&full
size=1

It actually doesn't look too different in size and shape than JEOL's
ultra-high-voltage TEMs:

http://hints.es.hokudai.ac.jp/en/instruments/img/image21.jpg
http://www.mext.go.jp/english/org/struct/image/029.jpg
http://www.icb.osaka-u.ac.jp/images/clip_image002.jpg

The scientist leading the tour calls it "largest electron microscope
on the eastern seaboard." The problem, though, is that it appears the
cell images shown on the screens come from a phase contrast
microscope, not a TEM or SEM. Presumably that was just to show the
cells moving.

All in all, it wasn't too bad a representation of electron microscopy
for a Hollywood blockbuster.

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010


==============================Original
Headers==============================
9, 20 -- From frah0010-at-umn.edu Tue Jun 16 11:21:16 2009
9, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu
[134.84.119.32])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5GGLFkn021853
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
11:21:16 -0500
9, 20 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu
[160.94.146.133])
9, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
11:21:14 -0500 (CDT)
9, 20 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133]
#+LO+TS+AU
9, 20 -- X-Umn-Classification: local
9, 20 -- Message-Id: {E9ACAB40-4B27-4C3F-BFA4-725927F53DDB-at-umn.edu}
9, 20 -- From: Ellery Frahm {frah0010-at-umn.edu}
9, 20 -- To: microscopy-at-microscopy.com
9, 20 -- In-Reply-To: {200906161559.n5GFx1la011281-at-ns.microscopy.com}
9, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
9, 20 -- Content-Transfer-Encoding: 7bit
9, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
9, 20 -- Subject: Re: [Microscopy] Fwd: RE: Lab envy?
9, 20 -- Date: Tue, 16 Jun 2009 11:21:13 -0500
9, 20 -- References: {200906161559.n5GFx1la011281-at-ns.microscopy.com}
9, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of -
Headers==============================


==============================Original Headers==============================
19, 28 -- From parmiterd-at-mail.nih.gov Tue Jun 16 12:29:09 2009
19, 28 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113])
19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GHT8nj019024
19, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 12:29:09 -0500
19, 28 -- X-IronPortListener: Outbound_SMTP
19, 28 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115])
19, 28 -- by nihxway5out.hub.nih.gov with ESMTP; 16 Jun 2009 13:28:56 -0400
19, 28 -- Received: from NIHCESMLBX8.nih.gov ([156.40.71.208]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
19, 28 -- Tue, 16 Jun 2009 13:28:56 -0400
19, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 28 -- Content-class: urn:content-classes:message
19, 28 -- MIME-Version: 1.0
19, 28 -- Content-Type: text/plain;
19, 28 -- charset="us-ascii"
19, 28 -- Subject: RE: Lab envy?
19, 28 -- Date: Tue, 16 Jun 2009 13:28:15 -0400
19, 28 -- Message-ID: {FE46A07FD9BDB64493F24C65B9667B49035B0EE6-at-NIHCESMLBX8.nih.gov}
19, 28 -- In-Reply-To: {200906161630.n5GGU9mE011918-at-ns.microscopy.com}
19, 28 -- X-MS-Has-Attach:
19, 28 -- X-MS-TNEF-Correlator:
19, 28 -- Thread-Topic: Lab envy?
19, 28 -- Thread-Index: Acnun7fxUmfs3/M+TEKcz7+63GbeHQABkpDQ
19, 28 -- References: {200906161630.n5GGU9mE011918-at-ns.microscopy.com}
19, 28 -- From: "Parmiter, David (NIH/NCI) [C]" {parmiterd-at-mail.nih.gov}
19, 28 -- To: {Microscopy-at-microscopy.com}
19, 28 -- X-OriginalArrivalTime: 16 Jun 2009 17:28:56.0211 (UTC) FILETIME=[ED216E30:01C9EEA7]
19, 28 -- Content-Transfer-Encoding: 8bit
19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GHT8nj019024
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 16 Jun 2009 13:26:42 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ellery,

Cool image! I haven't seen a scope like the one in the movie clip
before. However, based on the movie vintage and studio location, I
would suspect that it is a Vickers microscope. Metropolitan-Vickers
later became AEI. There is a photo of a Vickers EM-2 circa 1946 at
http://www.scienceandsociety.co.uk/results.asp?image=10307509

Cheers,
Henk

At 11:28 AM 06/16/09, frah0010-at-umn.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
9, 26 -- From colijn.1-at-osu.edu Tue Jun 16 13:26:41 2009
9, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GIQeZc004144
9, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 13:26:40 -0500
9, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
9, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
9, 26 -- id {01NA85PFPQR493G4UF-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
9, 26 -- Tue, 16 Jun 2009 14:26:36 -0400 (EDT)
9, 26 -- Received: from HOC1.ecr6.ohio-state.edu
9, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
9, 26 -- (PMDF V6.3-x18 #31556)
9, 26 -- with ESMTPA id {01NA85PG11JG953XUD-at-ecr6.ohio-state.edu} ; Tue,
9, 26 -- 16 Jun 2009 14:26:37 -0400 (EDT)
9, 26 -- Date: Tue, 16 Jun 2009 14:28:12 -0400
9, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
9, 26 -- Subject: Re: [Microscopy] Re: Lab envy?
9, 26 -- In-reply-to: {200906161528.n5GFSJYY027214-at-ns.microscopy.com}
9, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
9, 26 -- To: frah0010-at-umn.edu
9, 26 -- Cc: Microscopy-at-microscopy.com
9, 26 -- Message-id: {01NA85PG2GE6953XUD-at-ecr6.ohio-state.edu}
9, 26 -- MIME-version: 1.0
9, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
9, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
9, 26 -- References: {200906161528.n5GFSJYY027214-at-ns.microscopy.com}
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Tue, 16 Jun 2009 13:33:55 -0500
Subject: [Microscopy] Re: CPD and charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} So, we're going to try two things initially: 1) We're bringing both
} CPDs to Tina's lab for side-by-side testing until we get to the bottom
} of this, and 2) where do you buy rubber chickens?

OK, I'll clear a space next to my CPD. I know a mobile DJ/bartender team
that will come and supply the rubber chickens along with the drinks with
umbrellas. How many people are you bringing?

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
6, 21 -- From tina-at-pbrc.hawaii.edu Tue Jun 16 13:33:54 2009
6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GIXsnH012620
6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 13:33:54 -0500
6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n5GIXnSF018812
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
6, 21 -- Tue, 16 Jun 2009 08:33:50 -1000 (HST)
6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n5GIXnhs018809;
6, 21 -- Tue, 16 Jun 2009 08:33:49 -1000 (HST)
6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
6, 21 -- Date: Tue, 16 Jun 2009 08:33:48 -1000 (HST)
6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
6, 21 -- X-Sender: tina-at-halia
6, 21 -- To: TindallR-at-missouri.edu
6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 21 -- Subject: Re: [Microscopy] CPD and charging
6, 21 -- In-Reply-To: {200906161355.n5GDtU2j020354-at-ns.microscopy.com}
6, 21 -- Message-ID: {Pine.GSO.4.21.0906160831040.18745-100000-at-halia}
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Tue, 16 Jun 2009 13:34:59 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I stand corrected on the Hitachi scopes -- thanks for correcting that,
David. The first image is the JEOL JEM-ARM-1300, and the next two
were Hitachi H-3000. Both are called "ultra-high voltage electron
microscopes" so my Google image search turned up both. Sorry for any
confusion.

As for the Spiderman scope not needing maintenance, a lack of repairs
might be why there are radioactive spiders running around the lab.

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010





==============================Original Headers==============================
8, 20 -- From frah0010-at-umn.edu Tue Jun 16 13:34:59 2009
8, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GIYwRn015171
8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 13:34:58 -0500
8, 20 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133])
8, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 13:34:41 -0500 (CDT)
8, 20 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU
8, 20 -- X-Umn-Classification: local
8, 20 -- Message-Id: {FD6094AA-4549-4A68-B738-8672783D0688-at-umn.edu}
8, 20 -- From: Ellery Frahm {frah0010-at-umn.edu}
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- In-Reply-To: {200906161735.n5GHZLdV026903-at-ns.microscopy.com}
8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 20 -- Content-Transfer-Encoding: 7bit
8, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
8, 20 -- Subject: Re: [Microscopy] RE: Lab envy?
8, 20 -- Date: Tue, 16 Jun 2009 13:34:41 -0500
8, 20 -- References: {200906161735.n5GHZLdV026903-at-ns.microscopy.com}
8, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Tue, 16 Jun 2009 17:24:04 -0500
Subject: [Microscopy] Coolwell chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
I know Jeff Pare at Emory just asked for the schematics for a Coolwell
Chiller. It must be a southern thing cause today our Coolwell chiller/
water recirculator went out, too. It is a slightly different model.
Does anyone have schematics for a SE-080W CZ Coolwell? I would greatly
appreciate a copy.

Thanks for your consideration of this problemo.
Beth


==============================Original Headers==============================
3, 19 -- From beth-at-plantbio.uga.edu Tue Jun 16 17:24:04 2009
3, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GMO4IA024493
3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 17:24:04 -0500
3, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
3, 19 -- (authenticated user beth-at-plantbio.uga.edu)
3, 19 -- by dogwood.plantbio.uga.edu
3, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
3, 19 -- for microscopy-at-microscopy.com;
3, 19 -- Tue, 16 Jun 2009 18:23:57 -0400
3, 19 -- Message-Id: {35E71750-215C-4B39-A3A9-FA940E566BB1-at-plantbio.uga.edu}
3, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
3, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
3, 19 -- Content-Transfer-Encoding: 7bit
3, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
3, 19 -- Subject: Coolwell chiller
3, 19 -- Date: Tue, 16 Jun 2009 18:24:00 -0400
3, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Tue, 16 Jun 2009 18:24:19 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stephane,

Thank you for your appreciation. I do appreciate your slight
disagreement and would like to add that hopefully from difference of
opinions there will be in the end a useful contribution to explaining
the phenomenon that Robert describes.

I think you will agree that researchers have a responsibility to try
to explain differences that may arise from different approaches. I
would think it mandatory where the outcome of those approaches (being
LM and EM immunodetection) are different, but where nevertheless an
end result is put in the same wording "this or that antigen localizes
there and there". In Robert's case he has made a big effort to keep
many of the parameters involved very similar or even exactly the same.
And exactly that makes the difference that much more difficult to
understand and thus so interesting and challenging. In my opinion the
appropriate way forward would be to further eliminate the number of
remaining procedural parameters in an attempt to increase the
reliability of the outcome and to understand what's going on.

Allow me to comment to a few of the issues you raise.

I did not say antigens are usually less accessible in TEM. I stated
that 5 and 10 nm gold conjugates do not penetrate readily, even in
hydrated Tokuyasu sections. The solution is not using higher primary
concentrations but using a secondary reagent that penetrates more
easily.

What is the reason for using higher antibody concentrations, if you
assume antigens are only limitedly accessible, e.g. only at the
section surface? Those antigens do not "know" they are the only ones
available for binding. In general, any interaction between those
antigens and antibodies would only be different if there is a
difference in avidity binding, or when the antigen was altered to
affect affinity binding. That does not seem likely in the present
case, as the material was treated much the same, but ... often there
are differences without us being aware.

Although maybe not directly relevant for the observations of Robert, I
do agree that different antibody concentrations may result in
detecting different antigens/epitopes or molecular structures that are
very similar in surface structure. Or that the relative signal
densities may differ. This certainly can be the cause of
misinterpretations. Antibody concentrations determine what the
antibodies bind to. This raises the question: which concentration
(range) is the right one? I wish there was an easy answer, but there
isn't. In immunochemistry parameters and variables are much more easy
to establish and understand than in immunohisto- and
immunocytochemistry. In tissue, after fixation, dehydration, freezing,
thawing we are likely facing a vast range of modifications to the
original antigen, both physically and chemically. So being consistent,
comparing apples with apples, as Leona Cohen-Gould puts it, is the way
to go.

Strictly, finding a certain antigen pattern in cells does only mean
that that is the situation for those cells at best, under those
particular circumstances. Comparing localization in cells with tissues
should therefore be done with caution, but may certainly help.
However, using cells that lack certain structures and thus do not show
labeling of those structures.... hmmm...not sure about the relevance
of that one!

In my previous message I stated: "...it is very worthwhile to
understand what goes on. If not, what do we decide is real? The label
pattern we like, the one that suits us best?" Keeping an open mind for
what we see, for the results we obtain and making an effort to
understand those should also discriminate researchers from finders.


Cheers,

Jan


On 16/06/2009, at 9:04 PM, nizets2-at-yahoo.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Although I find the contributions of Mr Leunissen always very
} interesting, I don't completely agree on this one.
} If 2 methods demand different approaches, just approach them
} differently!
} Let's suppose that you are working with polyclonal antibodies, you
} may reasonable think that there exist a possibility for different
} recognition patterns.
} The antigens are usually less accessible in TEM (as stated by Mr
} Leunissen), which usually ends up in increased antibody
} concentrations. These are limitations that you cannot overcome, they
} are inherent to the technique.
} I would just optimize each technique independently.
} Now on the interpretation side: Perhaps (I said perhaps) the
} desmosome labeling was so intense that you did not pay enough
} attention to any other weak labeling. If you detect say 250
} particles/µm² in the desmosomes, 25 in nuclear compartments and 5 in
} the resin, the nuclear compartment is still specifically labeled!
} (although weaker)
} Or perhaps the antibody concentration is enough to label the
} desmosomes but not enough to label the nucleus (just increase the
} concentration further).
}
} You seem to have no idea what to expect for a labeling pattern. You
} could make a simple immunolabeling in different cells in culture, so
} you would get an idea of how the transcription factor is generally
} distributed using your antibody. (this would answer questions like
} "what labeling is real?")
} Normal keratinocytes and skin fibroblasts are available for cell
} culture (you lucky you). Keratinocytes may even be easily influenced
} just by changing the Ca2+ concentration in culture medium.
} Notwithstanding the fact that you will probably be freed from any
} unwanted desmosome labeling.
}
} Side note: it would be helpful (I think) to run a western blot with
} a cell extract and the antibody you use in microscopy. You may be
} able to distinguish several bands, some attributed to your specific
} antigen (transcription factor) and others to unspecific targets
} (desmosome components?).
}
} What really troubles me is that you have a strong labeling of
} desmosomes in EM and none in LM (or do you?). But hey if we could
} explain everything we would be finders and not researchers :-)
}
} Best regards,
} Stephane
}
}
}
} ----- Original Message ----
} X-from: "leunissen-at-aurion.nl" {leunissen-at-aurion.nl}
} To: nizets2-at-yahoo.com
} Sent: Friday, June 12, 2009 10:10:22 PM
} Subject: [Microscopy] Re: :LM vs TEM immunostain patterns
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Robert,
}
} Yes, I have seen this happen before, although in those case there were
} always two different detection systems used. What you describe as an
} example is very clear and extreme and I am not sure I have a solution.
} The only thing you can do to solve this is keep all steps and
} treatments rigorously the same except the visualization technique
} used. Have you tried to label semi-thin cryosections prepared with the
} Tokuyasu technique for LM? Using the smallest gold particles and
} silver enhancement? Did you use the same buffers, additives, protocol?
} Controls ok?
}
} Even in ultrathin cryosections there is only very limited penetration
} for gold conjugates when the particle size is 5 or 10 nm. You may
} remember from the Atlanta workshop that such conjugates only label the
} surface in general. The hydrodynamic size of LM reagents is usually
} smaller than that of larger gold particles and therefore LM
} secondaries penetrate more easily, so that is why the smallest
} particles are preferred if you want to reach those antigens inside
} hydrated sections for EM.
}
} It seems insane when you see those differences, I agree. I can not say
} that the penetration issue is the only reason, but it may be a start.
} I am certain many have had similar experiences and it is very
} worthwhile to understand what goes on. If not, what do we decide is
} real? The label pattern we like, the one that suits us best?
}
} Cheers, have a good weekend all!
}
}
}
} Jan Leunissen
}
}
} On 13/06/2009, at 4:12 AM, underwoo-at-u.washington.edu wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear Microscopists,
} }
} } I desire feedback on differences in immunolabeling patterns as
} } observed between light level cryosections and cryothin sections for
} } transmission electron microscopy. If I take the exact same tissue, a
} } piece of skin in this case, fix it in the same fixative, usually a
} } recipe containing buffered 2% para / 0.025% glut, for the same
} } fixation time, cryoprotect in sucrose for LM and PVP/sucrose for
} } TEM, then freeze and section 6 micron frozen sections for LM and
} } 100nm thick cryothin sections picked up on nickel formvar/carbon
} } grids for TEM, I get a completely different labeling pattern with
} } perhaps 10-20% of antibodies. Has anyone had similar observations?
} } If so, have you figured out why? I have tried to rule out PVP
} } (polyvinylpyrrolidone) as one of the obvious differences between the
} } two protocols but tissue cryoprotected with PVP/sucrose cannot be
} } cut at 6 microns in a cryostat. I have tried to
} } rule out all other differences and remain stumped. As example,
} } labeling for a particular transcription factor shows
} } compartmentalized staining in the nucleus at the LM level and at the
} } TEM level specifically stains intercellular desmosomal adhesions
} } between the keratinocytes of the epidermis. Crazy!
} }
} } Sincerely,
} } Robert Underwood
} } University of Washington
} } Dermatology
} } Seattle, WA
} }
} }
} }
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 22 -- From underwoo-at-u.washington.edu Fri Jun 12 11:11:51 2009
} } 9, 22 -- Received: from mxout3.cac.washington.edu
} } (mxout3.cac.washington.edu [140.142.32.166])
} } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP
} } id n5CGBmnS018118
} } 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009
} } 11:11:50
} } -0500
} } 9, 22 -- Received: from hymn13.u.washington.edu
} } (hymn13.u.washington.edu [140.142.4.103])
} } 9, 22 -- by mxout3.cac.washington.edu
} } (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5CGBj0l020228
} } 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} } verify=NO)
} } 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009
} } 09:11:47
} } -0700
} } 9, 22 -- Received: from localhost (localhost [127.0.0.1])
} } 9, 22 -- by hymn13.u.washington.edu
} } (8.14.3+UW09.03/8.14.3+UW09.03)
} } with ESMTP id n5CGBgSq019694
} } 9, 22 -- for {Microscopy-at-microscopy.Com} ; Fri, 12 Jun 2009
} } 09:11:42
} } -0700
} } 9, 22 -- X-Auth-Received: from [128.208.106.143] by
} } hymn13.u.washington.edu via HTTP; Fri, 12 Jun 2009 09:11:42 PDT
} } 9, 22 -- Date: Fri, 12 Jun 2009 09:11:42 -0700 (PDT)
} } 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
} } 9, 22 -- To: Microscopy List {Microscopy-at-microscopy.Com}
} } 9, 22 -- Subject: [Microscopy]:LM vs TEM immunostain patterns
} } 9, 22 -- Message-ID: {Pine.LNX.4.43.0906120911420.10631-at-hymn13.u.washington.edu
} } }
} } 9, 22 -- MIME-Version: 1.0
} } 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} } 9, 22 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594,
} } Antispam-Data: 2009.6.12.160138
} } 9, 22 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
} } 9, 22 -- SUPERLONG_LINE 0.05, BODY_SIZE_1300_1399 0,
} } BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
} } __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0,
} } __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0,
} } __TO_MALFORMED_2 0'
} } ==============================End of -
} } Headers==============================
}
}
} ==============================Original
} Headers==============================
} 12, 20 -- From leunissen-at-aurion.nl Fri Jun 12 15:06:32 2009
} 12, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz
} [210.54.141.244])
} 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5CK6Uk4012793
} 12, 20 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jun 2009
} 15:06:30 -0500
} 12, 20 -- Received: from [192.168.1.50] (really [122.57.248.181]) by
} fep02.xtra.co.nz
} 12, 20 -- with ESMTP
} 12, 20 -- id
} {20090612200628.PHMS28374.fep02.xtra.co.nz-at-[192.168.1.50]} ;
} 12, 20 -- Sat, 13 Jun 2009 08:06:28 +1200
} 12, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
} 12, 20 -- To: underwoo-at-u.washington.edu
} 12, 20 -- In-Reply-To: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
} 12, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
} 12, 20 -- References: {200906121612.n5CGCM0k018452-at-ns.microscopy.com}
} 12, 20 -- Message-Id: {490EE65A-A1CA-4DE4-B767-912DE666A018-at-aurion.nl}
} 12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes
} 12, 20 -- Content-Transfer-Encoding: 7bit
} 12, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
} 12, 20 -- Date: Sat, 13 Jun 2009 08:06:25 +1200
} 12, 20 -- Cc: microscopy-at-microscopy.com
} 12, 20 -- X-Mailer: Apple Mail (2.935.3)
} ==============================End of -
} Headers==============================
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 30, 25 -- From nizets2-at-yahoo.com Tue Jun 16 04:03:48 2009
} 30, 25 -- Received: from web110808.mail.gq1.yahoo.com
} (web110808.mail.gq1.yahoo.com [67.195.13.231])
} 30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} id n5G93lRB029336
} 30, 25 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
} 04:03:47 -0500
} 30, 25 -- Received: (qmail 75798 invoked by uid 60001); 16 Jun 2009
} 09:03:47 -0000
} 30, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} d=yahoo.com; s=s1024; t=1245143026; bh=MEFlES/
} hYtB7mhvbYuj6ygxpqZXqXHDArEbpwXTHeYI=; h=Message-ID:X-YMail-
} OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-
} To:MIME-Version:Content-Type:Content-Transfer-Encoding;
} b=budCPYcT83GtgbyG34Q3UDlKr7odRYBw84RfNztG8XsV9Dy/jrzLxIb0NA/
} 0EZ7Yi1
} +
} fFIXSGfJ7glyjosifZLg0UpmBxjiW7NfFAfX7iBGdIHQgc4QFO6R3hEjYvKd6CXBJTvWdv
} /okGyDrxMA7Ew1dSpyNPpyEcv2Oy0/DdoI=
} 30, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 30, 25 -- s=s1024; d=yahoo.com;
} 30, 25 -- h=Message-ID:X-YMail-OSG:Received:X-
} Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-
} Version:Content-Type:Content-Transfer-Encoding;
} 30, 25 -- b=llK3EwHbI/8hg9yGEjsoFrlJFY9DUe96GZgFFF3aEBT/ZwoIeEffb
} +erJed8IctMPkmqd6klXIMbza/otpCZ/p
} +KvfWWTHjhO3ziYBiHrRBlZxFHv4RVnkYvUCNArvYY/
} X0nJcT3GrULhchsTjRvY49CtZtzYEaVQhSxb1dkUDA=;
} 30, 25 -- Message-ID: {884995.75697.qm-at-web110808.mail.gq1.yahoo.com}
} 30, 25 -- X-YMail-OSG:
} XEyC1J8VM1kxxv6VvG6MuIT_6NnlHC1m1QTWVg2ZX9.6TJJeEbUfyeiKZO96Vs74hMqLFqfymNqUbVx2k52063lTsu6fGhSlM3Id8Xu71tGWIjvfPJuCrsqnTzvdd2yUL_45NHFZoVBb.XrhhRpJcS.ZlddL3i4koUcFG_OyBOA_RKFEduH6b8SHz1TCB1OllrJCBIg3fjXrEkJtng4Du1ToHx_aS_eSwimzkFmmnJ6T6OpdBfpjsHLyXtkYJD4hzERCN4m.mSkJwc8JtSZCQgsVvd24JYrcazgLpVKTzqKY.LWOHTqUxIMXwJuKQKZ.v2HQComWseIN5NCxwCq2ayIiSSEyRfQjcPgjmsNyNr8-
} 30, 25 -- Received: from [80.122.101.100] by
} web110808.mail.gq1.yahoo.com via HTTP; Tue, 16 Jun 2009 02:03:46 PDT
} 30, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
} 30, 25 -- References: {200906122010.n5CKAMZv024835-at-ns.microscopy.com}
} 30, 25 -- Date: Tue, 16 Jun 2009 02:03:46 -0700 (PDT)
} 30, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 30, 25 -- Subject: Re: [Microscopy] Re: :LM vs TEM immunostain
} patterns
} 30, 25 -- To: underwoo-at-u.washington.edu
} 30, 25 -- Cc: microscopy-at-microscopy.com
} 30, 25 -- In-Reply-To: {200906122010.n5CKAMZv024835-at-ns.microscopy.com}
} 30, 25 -- MIME-Version: 1.0
} 30, 25 -- Content-Type: text/plain; charset=iso-8859-1
} 30, 25 -- Content-Transfer-Encoding: 8bit
} 30, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n5G93lRB029336
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
17, 20 -- From leunissen-at-aurion.nl Tue Jun 16 18:24:19 2009
17, 20 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5GNOHNM010090
17, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 18:24:18 -0500
17, 20 -- Received: from [192.168.1.50] (really [122.57.247.101]) by fep01.xtra.co.nz
17, 20 -- with ESMTP
17, 20 -- id {20090616232411.KVCN3703.fep01.xtra.co.nz-at-[192.168.1.50]}
17, 20 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 11:24:11 +1200
17, 20 -- Message-Id: {EAFE7FC8-3F91-4D6B-AD93-B6D8D2850711-at-aurion.nl}
17, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
17, 20 -- To: microscopy-at-microscopy.com
17, 20 -- In-Reply-To: {200906160904.n5G94H6N029691-at-ns.microscopy.com}
17, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
17, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
17, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
17, 20 -- Date: Wed, 17 Jun 2009 11:24:10 +1200
17, 20 -- References: {200906160904.n5G94H6N029691-at-ns.microscopy.com}
17, 20 -- X-Mailer: Apple Mail (2.935.3)
17, 20 -- Content-Transfer-Encoding: 8bit
17, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5GNOHNM010090
==============================End of - Headers==============================




From: mmiralles-at-pi.ac.ae
Date: Tue, 16 Jun 2009 23:27:50 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wow! Great classic film!
These would be very useful for me in trying to convince students that I
am not the only one who's addicted to microscopy. =) Thank you for
citing all these movies/films!
I could vaguely remember the scene but I've seen an Denzel
Washington/Angelina Jolie film, "The Bone Collector" analyzes something
(sorry, forgot already what it is) using a table-top SEM/EDS.

Regards,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE


-----Original Message-----
X-from: frah0010-at-umn.edu [mailto:frah0010-at-umn.edu]
Sent: Tuesday, June 16, 2009 7:31 PM
To: Melina Miralles

I believe the "electron microscope" in "The Man in the White Suit" in
a TEM as seen here:

http://www.tcm.com/mediaroom/index.jsp?cid=222698

Someone here should be able to identify the manufacturer and model.

Unfortunately I've never seen an electron microprobe in a film -- let
me know if anyone ever finds one!

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010



==============================Original Headers==============================
17, 29 -- From mmiralles-at-pi.ac.ae Tue Jun 16 23:27:49 2009
17, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5H4RmPP006141
17, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 23:27:49 -0500
17, 29 -- X-IronPort-AV: E=Sophos;i="4.42,234,1243800000";
17, 29 -- d="scan'208";a="1179691"
17, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
17, 29 -- by mx1.pi.ac.ae with ESMTP; 17 Jun 2009 08:06:56 +0400
17, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
17, 29 -- Wed, 17 Jun 2009 08:26:39 +0400
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: Re: Lab envy?
17, 29 -- Date: Wed, 17 Jun 2009 08:26:27 +0400
17, 29 -- Message-ID: {D5603421C6303A46883F87E7968F285B0707EBF7-at-pi-exm.PI.AC.AE}
17, 29 -- In-Reply-To: {200906161531.n5GFVJ4A000398-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: Lab envy?
17, 29 -- Thread-Index: Acnul1jKJ356xezrTpq64vv6b0Pk6AAavJ7w
17, 29 -- References: {200906161531.n5GFVJ4A000398-at-ns.microscopy.com}
17, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
17, 29 -- To: {microscopy-at-microscopy.com}
17, 29 -- X-OriginalArrivalTime: 17 Jun 2009 04:26:39.0329 (UTC) FILETIME=[CEFB5D10:01C9EF03]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5H4RmPP006141
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 16 Jun 2009 23:52:44 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't see why any of us should give any time
or effort to this useless venue.

It is interesting to point out the stupid and
obnoxious disconnections in films. But so what?
We deal in reality while they deal in sureality.
Their literary licences allows them a great degree
of flexibility. As we have seen, much is fake.

Our surrounding comments are not going to make
any difference. I say, do our jobs, ignore these
idiots, dummies, etc.

If not, why not?

gary g.




==============================Original Headers==============================
8, 20 -- From gary-at-gaugler.com Tue Jun 16 23:52:44 2009
8, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5H4qhUK021093
8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009 23:52:43 -0500
8, 20 -- Message-Id: {200906170452.n5H4qhUK021093-at-ns.microscopy.com}
8, 20 -- Received: (qmail 5052 invoked from network); 16 Jun 2009 22:11:11 -0700
8, 20 -- Received: by simscan 1.1.0 ppid: 5049, pid: 5050, t: 0.2027s
8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
8, 20 -- by smtp1 with SMTP; 16 Jun 2009 22:11:10 -0700
8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 20 -- Date: Tue, 16 Jun 2009 21:52:39 -0700
8, 20 -- To: mmiralles-at-pi.ac.ae
8, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
8, 20 -- Subject: Re: [Microscopy] Re: Lab envy?
8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
8, 20 -- In-Reply-To: {200906170429.n5H4TVDw008514-at-ns.microscopy.com}
8, 20 -- References: {200906170429.n5H4TVDw008514-at-ns.microscopy.com}
8, 20 -- Mime-Version: 1.0
8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Wed, 17 Jun 2009 04:59:43 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Jan!

You say that gold conjugates have limited penetration and I say that antigens are less accessible. What is the difference?
I agree that "increasing the antibody concentration" was a bit hasty. Actually, having a general weaker signal (because of limited penetration), or to say otherwise having less antigens to recognize, you may wish to increase the signal in order to see something. I know that in this regard I have something to learn from you so I will always appreciate your comments on this matter but it all seems to make sense to me.

Now on the necessity to understand what happens: it is all a question of personal goals and priorities I think.
But I must say that it is my personal experience that it is better to have some limits to explorations. It happened in my career that I spent a lot of time to understand a phenomenon and in the end it did not bring much because that was not what I was looking for. Now I have become more pragmatic: I have a goal and I try not to let me disturb with whatever comes during the study. As researchers we are all curious in nature, but I think that we have to try and stay focused on our goals. In the end, what matters here is not to understand the precise mechanisms of labeling but to get a recognition patter of a transcription factor which makes sense in the given context.
In this case, if Robert can show a secondary "unspecific" band for a component of desmosomes in western blot, the staining of desmosomes in EM is OK, because it cannot interfere with a nuclear pattern (provided he has a specific nuclear pattern too). The absence of desmosome staining in LM does not need to be explained, it may be that upon thawing the antigens lost the conformation which allowed a correct immunodetection. Well there are possible explanations for the absence of staining in LM, they may remain as hypotheses because they do not interfere with the primary goal of location the transcription factor.

Finally, you wrote "However, using cells that lack certain structures and thus do not show labeling of those structures.... hmmm...not sure about the relevance of that one!". It IS relevant, as long as the-said features are not the object of study. If an antibody shows background staining in some cells and not in others, why not use the cells with low background staining (provided it still makes sense of course)? In the case I provided, I was talking about keratinocytes and skin fibroblasts because Robert is working with skin. Not because of the absence of desmosomes, but the absence of desmosomes is simply an addtional comfort.

Best regards,
Stéphane

 


----- Original Message ----
X-from: Jan Leunissen {leunissen-at-aurion.nl}
To: nizets2-at-yahoo.com
Sent: Wednesday, June 17, 2009 1:23:31 AM


Hi all
Just looked at a poster we have here & I think it's a Metropolitan
Vickers EM3A


Hi Ellery,

Cool image! I haven't seen a scope like the one in the movie clip
before. However, based on the movie vintage and studio location, I
would suspect that it is a Vickers microscope. Metropolitan-Vickers
later became AEI. There is a photo of a Vickers EM-2 circa 1946 at
http://www.scienceandsociety.co.uk/results.asp?image=10307509

Cheers,
Henk

At 11:28 AM 06/16/09, frah0010-at-umn.edu wrote:



} -----------------------------------------------------------------------
} ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} TEM as seen here:
}
} http://www.tcm.com/mediaroom/index.jsp?cid=222698
}
} Someone here should be able to identify the manufacturer and model.
}
} Unfortunately I've never seen an electron microprobe in a film -- let
} me know if anyone ever finds one!
}
} Best,
} Ellery
}
} -----------------
} Ellery Frahm
} Senior Research Fellow, Department of Geology & Geophysics Manager &
} Principal Analyst, Electron Microprobe Lab Doctoral Candidate,
} Department of Anthropology University of Minnesota - Twin Cities campus

} http://umn.edu/~frah0010

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
15, 35 -- From Nicola.Weston-at-nottingham.ac.uk Wed Jun 17 04:59:42 2009
15, 35 -- Received: from ixe-mta-23.emailfiltering.com (ixe-mta-23-tx.emailfiltering.com [194.116.199.156])
15, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5H9xfG3000732
15, 35 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 04:59:42 -0500
15, 35 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4])
15, 35 -- by ixe-mta-23.emailfiltering.com with emfmta (version 3.7.1.44.1.r-3.2.3-libc2.3.2) vanilla id 10390338
15, 35 -- for microscopy-at-microscopy.com; Wed, 17 Jun 2009 10:59:41 +0100
15, 35 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11])
15, 35 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60)
15, 35 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
15, 35 -- id 1MGrvO-0007v1-FV
15, 35 -- for microscopy-at-microscopy.com; Wed, 17 Jun 2009 10:59:22 +0100
15, 35 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
15, 35 -- Wed, 17 Jun 2009 10:58:26 +0100
15, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
15, 35 -- Content-class: urn:content-classes:message
15, 35 -- MIME-Version: 1.0
15, 35 -- Content-Type: text/plain;
15, 35 -- charset="us-ascii"
15, 35 -- Subject: Lab envy?
15, 35 -- Date: Wed, 17 Jun 2009 10:58:26 +0100
15, 35 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D9401A48D2E-at-VUIEXCHA.ad.nottingham.ac.uk}
15, 35 -- X-MS-Has-Attach:
15, 35 -- X-MS-TNEF-Correlator:
15, 35 -- Thread-Topic: Lab envy?
15, 35 -- Thread-Index: AcnvMiiZQsrjUQWvSS+FcX6SVdBczg==
15, 35 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
15, 35 -- To: {microscopy-at-microscopy.com}
15, 35 -- X-OriginalArrivalTime: 17 Jun 2009 09:58:26.0847 (UTC) FILETIME=[28C98AF0:01C9EF32]
15, 35 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
15, 35 -- X-UoN-MailScanner: Found to be clean
15, 35 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
15, 35 -- X-Spam-Status: No
15, 35 -- Content-Transfer-Encoding: 8bit
15, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5H9xfG3000732
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 17 Jun 2009 05:41:03 -0500
Subject: [Microscopy] compressed air drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all!

Due to my complete ignorance about all things cinema-oriented (I never watch TV, never go to the theater, instead I use my free time to make sport and yes sometimes I feel like an alien), here is something completely different:

We are the proud owners of a FEI Tecnai G20 and during his last visit, the FEI engineer told me that I should take care of the fact that the pressurized air used for the valves should not bring water in the instrument (I said air, not N2). He told me that the moisture produced is most of the time collected in the machine and one had to regularly empty the container otherwise it could enter the tubes and the instrument.
I had a look at our installation and it seems that nothing has been done to dry the compressed air.
So my question: what did YOU do to dry the compressed air? Silica?
An easy-to-do-it-yourself solution would be welcome.

Best regards,

Stéphane






==============================Original Headers==============================
10, 22 -- From nizets2-at-yahoo.com Wed Jun 17 05:41:02 2009
10, 22 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5HAf2KY016396
10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 05:41:02 -0500
10, 22 -- Received: (qmail 62189 invoked by uid 60001); 17 Jun 2009 10:41:02 -0000
10, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245235262; bh=QqHkZjN9KyvNRPOt34+d3CASWTbrZIYo5hR/RHNBWMY=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=qoontMyQG6D9K/kTnfS6YFW34O5d11Mg+QymQVH3d9JIbY4ThvYPMU9oYKD0dNsAik/x9nFE/ieJyU9wzB3ykAKof0htiQacnboxq2xDHdSWwt+MFcdBQTUKr/KbKE8oUahHZ6n4CxezTinG9vipfR2QrG8W/mXd6zIfcSNwtjg=
10, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 22 -- s=s1024; d=yahoo.com;
10, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
10, 22 -- b=dQB3Djj/KphZZN1On6I2Oz4PBr74Jvwc1/JZDM68SinxWojO923Q8Jfeb0MkeOUiQenRskVT0WiijJC6CCLhdUtlZShY01KyBFdG4/0MgO9k2s6XGoXxl+ZYUFVAgryPP2QK03HSiRwIa8W8EZBsrsvwse2EERjeFWULpG1eSXc=;
10, 22 -- Message-ID: {67977.62014.qm-at-web110814.mail.gq1.yahoo.com}
10, 22 -- X-YMail-OSG: uIlZTdkVM1m9mcYVbFlPQ0mRmE57pQUHTQpgS8EC2c_HK0saacCOV0AuD5zvISTQheFadVdOat6pvSR9nNJRkicFMYmUIHrEyOJn0ooVppLQ.TpNhWHdmeFCk3bKCJWZx2TBQr_9lQXaKpyMLUDn3ohkPySXe_lAQtRnnxs2J2ctxEFcvlnmOnlJuHy0DL3aZNX40C9WjeUWIdOcaK.ECin224FlXEpzi3_njOF7TAWrwGvwqMQQuc7WAIJukENPbpyzEVZMk.YhStNZCQhHAo_8uQT9Wu..J.C8rF7AyyEvVtl0G3Fp0sTOWKIxGarVrThZTqTa
10, 22 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Wed, 17 Jun 2009 03:41:01 PDT
10, 22 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
10, 22 -- Date: Wed, 17 Jun 2009 03:41:01 -0700 (PDT)
10, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
10, 22 -- Subject: compressed air drying
10, 22 -- To: microscopy-at-microscopy.com
10, 22 -- MIME-Version: 1.0
10, 22 -- Content-Type: text/plain; charset=iso-8859-1
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5HAf2KY016396
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Wed, 17 Jun 2009 05:55:08 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Why?
Great advances in knowledge have been accomplished by answering the
child-like question, why?

But not today.

This conversation is simply harmless, good-natured fun. And that, with all
the other problems facing us, our children and tour children's great grand
children, is reason enough.

stay safe........
Frank

PS: some of us just like taking an e-mail break........





gary-at-gaugler.com

06/17/2009 12:58 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
gary-at-gaugler.com [Microscopy] Lab envy?













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


I don't see why any of us should give any time
or effort to this useless venue.

It is interesting to point out the stupid and
obnoxious disconnections in films. But so what?
We deal in reality while they deal in sureality.
Their literary licences allows them a great degree
of flexibility. As we have seen, much is fake.

Our surrounding comments are not going to make
any difference. I say, do our jobs, ignore these
idiots, dummies, etc.

If not, why not?

gary g.




==============================Original
Headers==============================
8, 20 -- From gary-at-gaugler.com Tue Jun 16 23:52:44 2009
8, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n5H4qhUK021093
8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2009
23:52:43 -0500
8, 20 -- Message-Id: {200906170452.n5H4qhUK021093-at-ns.microscopy.com}
8, 20 -- Received: (qmail 5052 invoked from network); 16 Jun 2009 22:11:11
-0700
8, 20 -- Received: by simscan 1.1.0 ppid: 5049, pid: 5050, t: 0.2027s
8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
8, 20 -- by smtp1 with SMTP; 16 Jun 2009 22:11:10 -0700
8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 20 -- Date: Tue, 16 Jun 2009 21:52:39 -0700
8, 20 -- To: mmiralles-at-pi.ac.ae
8, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
8, 20 -- Subject: Re: [Microscopy] Re: Lab envy?
8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
8, 20 -- In-Reply-To: {200906170429.n5H4TVDw008514-at-ns.microscopy.com}
8, 20 -- References: {200906170429.n5H4TVDw008514-at-ns.microscopy.com}
8, 20 -- Mime-Version: 1.0
8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
30, 22 -- From frank_karl-at-lincolnelectric.com Wed Jun 17 05:55:07 2009
30, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
30, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HAt7C8030926
30, 22 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 05:55:07 -0500
30, 22 -- In-Reply-To: {200906170458.n5H4w6v5001297-at-ns.microscopy.com}
30, 22 -- Subject: Re: [Microscopy] Lab envy?
30, 22 -- To: Microscopy-at-microscopy.com
30, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
30, 22 -- Message-ID: {OF434FC118.139C5637-ON852575D8.003B7649-852575D8.003BF2BB-at-lincolnelectric.com}
30, 22 -- Date: Wed, 17 Jun 2009 06:54:53 -0400
30, 22 -- From: Frank_Karl-at-lincolnelectric.com
30, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 06/17/2009 06:54:54 AM,
30, 22 -- CD-MIME complete at 06/17/2009 06:54:54 AM,
30, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 06/17/2009 06:54:54 AM,
30, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
30, 22 -- 07, 2008) at 06/17/2009 06:54:54 AM,
30, 22 -- Serialize complete at 06/17/2009 06:54:54 AM
30, 22 -- MIME-Version: 1.0
30, 22 -- Content-Type: text/plain;
30, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Wed, 17 Jun 2009 07:07:34 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

gary-at-gaugler.com wrote:

} I don't see why any of us should give any time or effort to this
} useless venue. . .

One reason we should care about the portrayal of microscopy in films
and TV (other than simply amusing ourselves) is that it influences how
the public thinks about microscopy, science, research, etc. I suspect
that more people have seen, say, "Spiderman" than have ever seen or
used a real electron microscope or even had the use and function of
one explained to them. Millions of people watch CSI every week, and
these people are voters, taxpayers, jury members, and consumers of all
sorts of scientific and medical information, from global warming to
the H1N1 outbreak. The portrayal of microscopy and science in popular
culture is how many people get their information, however right or
wrong.

If you're looking for how sureality can affect reality, consider the
so-called CSI Effect:

http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
http://www.thepocketpart.org/2006/02/thomas.html

If our clients or students see microscopy and science portrayed badly
in films and TV, they can carry those expectations into the real
world. Many new users of the electron microprobe in my lab expect it
to operate under what I call the "microwave oven paradigm" -- open the
door and put in a sample, press one or two settings, hit the "Analyze"
button, wait a minute or two, and you're done! I haven't done any
surveys to learn exactly how they form such misconceptions, but I'd
hardly be surprised if the portrayal of scientific instruments on TV
had a lot to do with it.

The point of the discussion isn't really to change Hollywood. In
fact, I'd argue that we shouldn't -- even I don't want to watch five
minutes of doing a sample change in the middle of a film before the
scientist identifies the mysterious material as otherworldly.
Instead, we should be aware of the misconceptions that many people
have and that we need to expel before teaching them to use the
instrument or handing them their data. Scientists need to be more
aware of and involved in the public perception of science.

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010





==============================Original Headers==============================
13, 19 -- From frah0010-at-umn.edu Wed Jun 17 07:07:33 2009
13, 19 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HC7VNN015839
13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 07:07:31 -0500
13, 19 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206])
13, 19 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 07:07:31 -0500 (CDT)
13, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN
13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
13, 19 -- To: microscopy-at-microscopy.com
13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
13, 19 -- Content-Transfer-Encoding: 7bit
13, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 19 -- Subject: Re: [Microscopy] Lab envy?
13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
13, 19 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Wed, 17 Jun 2009 07:42:09 -0500
Subject: [Microscopy] Respect the subject line (was: Lab envy?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
If you don't want to follow this thread, don't open and read
the messages.

That intrusion of SEMs and TEMs into film is an appropriate
subject for this forum is given by the lively and wide-ranging
discussion it prompted. I delete unread all posts with EDS in the
subject line but I am not going to rain on any EDS thread, however
long-winded and multiposting it might become. That's why we have
subject lines.

Tobias
}
}
} I don't see why any of us should give any time
} or effort to this useless venue.
}
} It is interesting to point out the stupid and
} obnoxious disconnections in films. But so what?
} We deal in reality while they deal in sureality.
} Their literary licences allows them a great degree
} of flexibility. As we have seen, much is fake.
}
} Our surrounding comments are not going to make
} any difference. I say, do our jobs, ignore these
} idiots, dummies, etc.
}
} If not, why not?
}
} gary g.
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
4, 21 -- From baskin-at-bio.umass.edu Wed Jun 17 07:42:09 2009
4, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HCg9hq031410
4, 21 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 07:42:09 -0500
4, 21 -- Received: from [172.30.52.170] (eutopia [128.119.55.30])
4, 21 -- (authenticated bits=0)
4, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n5HCbVob025337
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
4, 21 -- Wed, 17 Jun 2009 08:37:32 -0400 (EDT)
4, 21 -- Mime-Version: 1.0
4, 21 -- Message-Id: {p06240502c65e9250c6a1-at-[172.30.52.170]}
4, 21 -- In-Reply-To: {200906170453.n5H4r6Kj021911-at-ns.microscopy.com}
4, 21 -- References: {200906170453.n5H4r6Kj021911-at-ns.microscopy.com}
4, 21 -- Date: Wed, 17 Jun 2009 08:37:29 -0400
4, 21 -- To: gary-at-gaugler.com
4, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 21 -- Subject: Respect the subject line (was: Lab envy?)
4, 21 -- Cc: microscopy-at-microscopy.com
4, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Wed, 17 Jun 2009 08:37:32 -0400 (EDT)
4, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Wed, 17 Jun 2009 08:03:47 -0500
Subject: [Microscopy] Re: compressed air drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stéphane,

Normally when you compress air, water condenses in the air compressor's
storage tank. It needs to be drained periodically. If not drained, it can
corrode the tank from the inside and water will get into the air delivery
lines.
To spray coatings, painters install an device called an 'extractor' in the
delivery line. Binks and Devilbiss make some nice extractors or condensate
separators. They also can be drained. Mine rarely had to be drained.
Grainger (on-line) even sells a refrigerated compressed air dryer, a
regenerative desiccant compressed air dryer, and a simple Dayton brand
silica gel desiccant as an in line dryer. I would try the later and see
how long the desiccant lasts and how bad the problem really is.

The real question here is, "How is that much water getting into the lines?"
I would suspect the storage tank is not being drained by your facilities
engineers or the automatic drain is not working properly.

Also, any iron pipes might be rusted and you might want to install a filter
to keep iron oxide particles from entering the pneumatics.
I once used a vial or beaker filled with cotton to check for rust in the
air or nitrogen lines. Just run a short piece of rubber tubing with an eye
dropper on the end into the cotton. Let the air line 'bleed' overnight.
In the morning, look for any rust stain in the cotton.

Paul

At 05:41 AM 6/17/09 -0500, nizets2-at-yahoo.com wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
6, 28 -- From beaurega-at-westol.com Wed Jun 17 08:03:47 2009
6, 28 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16])
6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HD3liJ013906
6, 28 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 08:03:47 -0500
6, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
6, 28 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id n5HD3dCZ002263
6, 28 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 09:03:43 -0400
6, 28 -- Received: (qmail 16183 invoked by uid 89); 17 Jun 2009 13:03:31 -0000
6, 28 -- Received: from pitts-69-72-21-55.dynamic-dialup.coretel.net (HELO running) (69.72.21.55)
6, 28 -- by mail.winbeam.com with SMTP; 17 Jun 2009 13:03:31 -0000
6, 28 -- Message-Id: {3.0.6.32.20090617090329.007af520-at-pop3.norton.antivirus}
6, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified)
6, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
6, 28 -- Date: Wed, 17 Jun 2009 09:03:29 -0400
6, 28 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com
6, 28 -- From: Beaurega {beaurega-at-westol.com}
6, 28 -- Subject: Re: [Microscopy] compressed air drying
6, 28 -- In-Reply-To: {200906171041.n5HAfOL0017012-at-ns.microscopy.com}
6, 28 -- Mime-Version: 1.0
6, 28 -- Content-Type: text/plain; charset="iso-8859-1"
6, 28 -- Content-Transfer-Encoding: 8bit
6, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
6, 28 -- X-Winbeam-MailScanner-ID: n5HD3dCZ002263
6, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
6, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted),
6, 28 -- SpamAssassin (not cached, score=-1.844, required 4, AWL 0.16,
6, 28 -- BAYES_00 -2.00)
6, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Wed, 17 Jun 2009 09:34:31 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,

I respect you a great deal, and you've been very helpful to me, so I
don't want this to seem like it's "pile-on Gary" time, but...I think
you're wrong.

The "CSI Effect" and depictions of microscopy specifically but also lab
work generally greatly affects my job, and probably that of others on
the list. It's the reason why I get requests/demands to explain why I
can't complete a 14 week project in 2 weeks, and why my bosses' boss
expects us to be able to do a full competitive product analysis and
deformulation in an hour. It's why visitors, when they see our brand-new
FEI Phenom, are disappointed that it has such a tiny, low-tech (to them)
touch screen - because they expect us to have the enormous,
semi-transparent, multi-touch screens that they've seen on CSI Miami.
(We don't even mention the fact that such a screen, even if you could
buy one, would cost significantly more than the Phenom did, and require
more computing power to operate) It's also one of the reasons why it is
so difficult to get permission to add new staff members to our group.

I'm not saying that we need to advocate for more accurate depictions of
science in the popular media either - just that we need to be aware of
them so that we know how and why to explain to non-scientists that the
science and/or the timing of the science that they see isn't real.

Best regards,

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company
Shelbyville, TN 37160
Direct: +1 (931) 685-6635

This message is intended for the Microscopy Listserv, and is the opinion
of the writer only. Permission is specifically granted to the Microscopy
Society of America to publish some or all of this message in the
Microscopy Today journal.

-----Original Message-----

I can't disagree with any of it. But then,

The public believes:
That ordinary police discharge their handguns at criminals several times a
year,
a person recovers from a gun shot in a less than TV month
district attorneys try and win 99% of every arrest
brilliant doctors constantly save every patient while angering patients and
support staff
Spies/agents are always good-looking, dashing and resourceful and leave a
undetected trail of bodies
WWII prisoners of war constantly got the upper hand on their captors and
ran resistance cells from the camp
Bosses are stupid and out witted by carefree employees..

Must I go on....

Entertainment almost always departs from reality and presents a distorted
view. If it didn't, it would be a documentary and not what most of us
would call entertainment.

Can we do anything about it? Yes, starting with answering questions from
friends who say..."Did you see... Can they really do that?"
Offer to lecture at high Schools, Civic organizations and book clubs.

stay safe.........






frah0010-at-umn.edu

06/17/2009 08:16 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
frah0010-at-umn.edu [Microscopy] Re: Lab envy?













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


gary-at-gaugler.com wrote:

} I don't see why any of us should give any time or effort to this
} useless venue. . .

One reason we should care about the portrayal of microscopy in films
and TV (other than simply amusing ourselves) is that it influences how
the public thinks about microscopy, science, research, etc. I suspect
that more people have seen, say, "Spiderman" than have ever seen or
used a real electron microscope or even had the use and function of
one explained to them. Millions of people watch CSI every week, and
these people are voters, taxpayers, jury members, and consumers of all
sorts of scientific and medical information, from global warming to
the H1N1 outbreak. The portrayal of microscopy and science in popular
culture is how many people get their information, however right or
wrong.

If you're looking for how sureality can affect reality, consider the
so-called CSI Effect:

http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
http://www.thepocketpart.org/2006/02/thomas.html

If our clients or students see microscopy and science portrayed badly
in films and TV, they can carry those expectations into the real
world. Many new users of the electron microprobe in my lab expect it
to operate under what I call the "microwave oven paradigm" -- open the
door and put in a sample, press one or two settings, hit the "Analyze"
button, wait a minute or two, and you're done! I haven't done any
surveys to learn exactly how they form such misconceptions, but I'd
hardly be surprised if the portrayal of scientific instruments on TV
had a lot to do with it.

The point of the discussion isn't really to change Hollywood. In
fact, I'd argue that we shouldn't -- even I don't want to watch five
minutes of doing a sample change in the middle of a film before the
scientist identifies the mysterious material as otherworldly.
Instead, we should be aware of the misconceptions that many people
have and that we need to expel before teaching them to use the
instrument or handing them their data. Scientists need to be more
aware of and involved in the public perception of science.

Best,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
Doctoral Candidate, Department of Anthropology
University of Minnesota - Twin Cities campus
http://umn.edu/~frah0010





==============================Original
Headers==============================
13, 19 -- From frah0010-at-umn.edu Wed Jun 17 07:07:33 2009
13, 19 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu
[134.84.119.32])
13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5HC7VNN015839
13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
07:07:31 -0500
13, 19 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net
[75.72.182.206])
13, 19 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
07:07:31 -0500 (CDT)
13, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net
[75.72.182.206] #+TS+AU+HN
13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
13, 19 -- To: microscopy-at-microscopy.com
13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
13, 19 -- Content-Transfer-Encoding: 7bit
13, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 19 -- Subject: Re: [Microscopy] Lab envy?
13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
13, 19 -- X-Mailer: Apple Mail (2.935.3)
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
37, 23 -- From frank_karl-at-lincolnelectric.com Wed Jun 17 09:34:31 2009
37, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
37, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HEYUUt015681
37, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 09:34:31 -0500
37, 23 -- In-Reply-To: {200906171216.n5HCGEvf028336-at-ns.microscopy.com}
37, 23 -- Subject: Lab envy?
37, 23 -- Sensitivity:
37, 23 -- To: Microscopy-at-microscopy.com
37, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
37, 23 -- Message-ID: {OFD94177D7.32F79557-ON852575D8.004434AB-852575D8.00500726-at-lincolnelectric.com}
37, 23 -- Date: Wed, 17 Jun 2009 10:34:13 -0400
37, 23 -- From: Frank_Karl-at-lincolnelectric.com
37, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
37, 23 -- CD-MIME complete at 06/17/2009 10:34:13 AM,
37, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
37, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
37, 23 -- Serialize complete at 06/17/2009 10:34:13 AM
37, 23 -- MIME-Version: 1.0
37, 23 -- Content-Type: text/plain;
37, 23 -- charset="US-ASCII"
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 17 Jun 2009 13:29:03 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well said, Frank, Ellery, Tobias, Robert. We have reasons to care about
the protrayal of microscopy in the media. Plus the thread is fun. It is so
much fun that I shared it with a couple of other people in the lab
yesterday, and finished with the comment that, since it was fun, some
crank was sure to object. We were wondering who it would be...

Aloha,
Tina

P.S.; my DELETE button is really easy to reach
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
4, 20 -- From tina-at-pbrc.hawaii.edu Wed Jun 17 13:29:03 2009
4, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HIT2vf007068
4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 13:29:03 -0500
4, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
4, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n5HISwmY022649
4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 08:28:59 -1000 (HST)
4, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n5HISvdk022646
4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 08:28:57 -1000 (HST)
4, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
4, 20 -- Date: Wed, 17 Jun 2009 08:28:57 -1000 (HST)
4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
4, 20 -- X-Sender: tina-at-halia
4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
4, 20 -- Subject: Re: [Microscopy] Lab envy?
4, 20 -- In-Reply-To: {200906170453.n5H4rQDr022597-at-ns.microscopy.com}
4, 20 -- Message-ID: {Pine.GSO.4.21.0906170825500.22606-100000-at-halia}
4, 20 -- MIME-Version: 1.0
4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Wed, 17 Jun 2009 17:41:05 -0500
Subject: [Microscopy] FIB Source Survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is to anyone who uses an FEI FIB or similar system using a
Gallium based Liquid Metal Ion Source(LMIS).

I'd like to know what the consensus is on whether to leave the source
running during the work week verse shutting it down every night.

We've currently been running ours doing overnight cuts or leaving it
idle, but on and blanked set to the highest aperture, for the night.
Usually we only turn it off for the weekends. Our last source lasted
roughly 6 months and had about 5970uAh on it. We've found that by
leaving it on the source seems more stable and is better able to stay
at 2.2uA over longer periods with little to no adjustments.

We have an old FIB820 here which isn't able to automatically control
the emission. So good stability is key for our overnight cutting. We
also do 90% of our cuts at 11.5nA.

Your two cents please?

==============================Original Headers==============================
5, 31 -- From ian.drucker-at-gmail.com Wed Jun 17 17:41:04 2009
5, 31 -- Received: from mail-yx0-f198.google.com (mail-yx0-f198.google.com [209.85.210.198])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HMf4Hn002223
5, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 17:41:04 -0500
5, 31 -- Received: by yxe36 with SMTP id 36so1003368yxe.10
5, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 15:41:04 -0700 (PDT)
5, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=domainkey-signature:mime-version:received:from:date:message-id
5, 31 -- :subject:to:content-type:content-transfer-encoding;
5, 31 -- bh=Yq3W0UKumN1vLYKcP92iegN+EE1wwcP6zAYEy1AhZJ0=;
5, 31 -- b=Bb7TQ5GjF+hkuh8IGxAT3u5vsvHRPpY19EdCfIMX/Z+rhd88gFc3NHNPjwdJal6gV8
5, 31 -- ZF4x7/rw/FqJeQbXjt4Gn3zdYJwIQvSGEVWRP+cLwX6r5SwJjVl6Ky6SiVjgq+PoQSAB
5, 31 -- Lk6u0Z1+usmAGiNejiEWaTOUclvibsFMVAbwM=
5, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 31 -- d=gmail.com; s=gamma;
5, 31 -- h=mime-version:from:date:message-id:subject:to:content-type
5, 31 -- :content-transfer-encoding;
5, 31 -- b=c/3avllEV3Penb196ny7heT3Vy60Mh5riOshHW5ngGMNKSGUbFHo5E5HzrTelrmCAQ
5, 31 -- 7j1SGO13Q2HQN1H8hRsJ4diSHmO3Q+IfDyvV3IWms/aRQT31hNE6SjbFj6nYMYPtOHEe
5, 31 -- PkKZhMpeIh5qt5QZVEWNbFSxCAFFn94m2da+Q=
5, 31 -- MIME-Version: 1.0
5, 31 -- Received: by 10.100.9.18 with SMTP id 18mr1011615ani.122.1245278463228; Wed,
5, 31 -- 17 Jun 2009 15:41:03 -0700 (PDT)
5, 31 -- From: ID {ian.drucker-at-gmail.com}
5, 31 -- Date: Wed, 17 Jun 2009 16:40:43 -0600
5, 31 -- Message-ID: {c0bfda950906171540r78d67a86rb4971fcdab6b1ae6-at-mail.gmail.com}
5, 31 -- Subject: FIB Source Survey
5, 31 -- To: Microscopy-at-microscopy.com
5, 31 -- Content-Type: text/plain; charset=ISO-8859-1
5, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: henrik.kaker-at-guest.arnes.si
Date: Wed, 17 Jun 2009 21:04:30 -0500
Subject: [Microscopy] viaWWW: SEM-EDS Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both henrik.kaker-at-guest.arnes.si as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: henrik.kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: SEM-EDS Lab

Title-Subject: [Filtered] Procedure for replacing rubber seal in Jeol
RP vacuum pump

Question: Bellow is the procedure for replacing the rubber seal in
Jeol JSM35C (CF) RP-100 vacuum pump.

1. Please remove the oil from the pump
( 1.5 ltr ) , keep it or change it .

2. Beat against the wheel from the V belt -
carefully on the right and on the left - with a
rubber hammer, to loosen the wheel from
the axel-drive shaft .
Please be careful, it comes suddenly that
you can pull the wheel from the shaft .

3. Now you can see the rubber seal around
the axel - drive shaft. The rubber seal is
protected with a thin metal ring.
You can loosen these ring with a special
tong .

4. You can see , that the old rubber seal is
completely rubber. The new one is from
metal, only around the shaft it is rubber .

5. Now , the onliest way to remove the old
rubber seal is, to destroy him !
You can do so with a stabel ( stundy )
screwdriver .

6. If you have done so like above, you have to
clean now the hole around the shaft ( the
place , where the rubber seal was been )

7. To bring in the new rubber seal , you can do
so with a special round tool.
Again please use a hammer and
tacker / hammer carefully with this tool
around and around the seal, till he is sitting
well in the hole - I am sorry, but you need a
bit experience for this work .

8. Fix the new rubber seal with the thin metal
ring ( ref. pos. 3 )

9. Install the wheel for the V - belt , and
hammer
him thigt ( rubber Hammer ) , mount the
6 / 8 mm hexagon screw in the middle of the
wheel .

10.Give the pump new fresh RP oil
( Pfeiffer P 3 oil )

Henrik

Login Host: 89.212.22.21
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Wed Jun 17 21:04:30 2009
17, 11 -- Received: from [12.154.81.156] (msdvpn072.msd.anl.gov [130.202.238.72])
17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5I24SYg021820
17, 11 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 21:04:29 -0500
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240806c65f510f9821-at-[12.154.81.156]}
17, 11 -- Date: Wed, 17 Jun 2009 21:04:32 -0500
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: henrik.kaker-at-guest.arnes.si (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: SEM-EDS Lab
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 17 Jun 2009 23:53:53 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, as an EE, fun means different dimensions I
suppose from others. But as Roseanne Roseannadanna
said, "Nevermind."

gary g.



At 11:31 AM 6/17/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 18 -- From gary-at-gaugler.com Wed Jun 17 23:53:53 2009
9, 18 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5I4rpWj010652
9, 18 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 23:53:52 -0500
9, 18 -- Message-Id: {200906180453.n5I4rpWj010652-at-ns.microscopy.com}
9, 18 -- Received: (qmail 32007 invoked from network); 17 Jun 2009 21:44:57 -0700
9, 18 -- Received: by simscan 1.1.0 ppid: 32004, pid: 32005, t: 0.1046s
9, 18 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 18 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 18 -- by smtp2 with SMTP; 17 Jun 2009 21:44:57 -0700
9, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 18 -- Date: Wed, 17 Jun 2009 21:53:44 -0700
9, 18 -- To: tina-at-pbrc.hawaii.edu
9, 18 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 18 -- Subject: [Microscopy] Re: Lab envy?
9, 18 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 18 -- Mime-Version: 1.0
9, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Thu, 18 Jun 2009 00:05:00 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stéphane,

This is a very interesting discussion and I hope it will stimulate
more responses from other members as we go "more fundamental". We are
perhaps moving away a little from the original thread. However, the
topic you addressed in the first line of your message challenges me to
give further explanations.

Even though the difference between limited penetration and limited
accessibility is fundamental, the end result may be the same and I
assume that is what you refer to. Both result in low signals,
sometimes too low to be convincing. To increase labeling efficiency it
is important to understand the mechanisms. Specimen characteristics
and specimen preparation control the environment of the antigen. When
antigens are encapsulated and therefore inaccessible, than that may be
something one has to accept for instance with antigens in acrylate
sections. On the other hand sometimes procedures can be applied that
expose masked antigens, e.g. by etching. Limited penetration on the
other hand is related to tissue characteristics as well as to the
reagents, and the development of smaller secondary gold conjugates
such as Auroprobe One, Undecagold, Nanogold and UltraSmall Gold
conjugates aimed at increasing labeling efficiency, and successfully so.

The pragmatic approach is a personal choice, certainly, and it is a
compromise. There may be many reasons why one would choose for a
pragmatic approach, one being the pressure on research funding. I
experience that in projects I am involved with too and it does not
always make me happy to be frank. As is the case with any compromise,
the chance for a pragmatic approach to be successful depends on how
well-informed one is about the options and this is where I hope to
contribute. Personally, whenever possible I choose the way that
explores more in-depth, in product development for instance. Going in-
depth in one area is probably what allows being pragmatic in another
area.

When you say "In the end, what matters here is not to understand the
precise mechanisms of labeling but to get a recognition patter of a
transcription factor which makes sense in the given context" I have to
disagree, especially with the second part. This tends to degenerate EM
to a confirmative technique rather than the unique research technique
status it deserves when employed properly. If we only look for what we
or the PI want to see because it fits nicely in existing concepts,
then we are bound to make only very limited progress, if at all and
why would we do this in the first place? From my personal experience I
know many core facilities find themselves confronted with this issue
and they probably often struggle to get the message across. To me this
seems a very fundamental issue.
When I was still associated with Utrecht university, longer ago than I
like to remember, we did some localization work in a collaboration
project with the microbiology dept. This was in the early days of
genetic engineering, involving multi-copy gene encoded hybrid proteins
which were hoped to be excreted or at least to be built into the outer
membrane of E coli. Biochemical studies using cell fractionation
indicated the hybrid proteins were in the membrane fraction.
Microbiologists happy .... and could we please make them a few
pictures, because it would look nice in the publication! The comic
book approach. Our localization work with immunogold revealed that the
hybrid protein was not IN the membrane but stacked below it on the
cytoplasmic side, blocking export sites etc. It took us a few meetings
to convey the message, you can probably well imagine. I think this
illustrates nicely how a confirmative approach would have made us give
up after a while or would have our immuno EM results to be discarded,
because the microbiologists thought they did not fit and did not like
it. Likewise, people coming from a light microscopy immuno background
often expect EM to give them the same results they obtained in the
light microscope. Without knowing how those results were obtained and
what they are worth, that can be hard! And here we are ... back to the
original topic.

One thing I admit: pragmaticians make for shorter messages!

Best regards everyone, we're heading towards the midwinter solstice
this weekend in New Zealand with a lantern parade and fireworks and
then the days will start to lengthen again!! Yay!
Enjoy the longest day and the magical fires on mountain ridges in some
areas of the Alps in the Northern hemisphere.


Jan


On 17/06/2009, at 8:52 PM, Stephane Nizet wrote:

}
} Hello Jan!
}
} You say that gold conjugates have limited penetration and I say that
} antigens are less accessible. What is the difference?
} I agree that "increasing the antibody concentration" was a bit
} hasty. Actually, having a general weaker signal (because of limited
} penetration), or to say otherwise having less antigens to recognize,
} you may wish to increase the signal in order to see something. I
} know that in this regard I have something to learn from you so I
} will always appreciate your comments on this matter but it all seems
} to make sense to me.
}
} Now on the necessity to understand what happens: it is all a
} question of personal goals and priorities I think.
} But I must say that it is my personal experience that it is better
} to have some limits to explorations. It happened in my career that I
} spent a lot of time to understand a phenomenon and in the end it did
} not bring much because that was not what I was looking for. Now I
} have become more pragmatic: I have a goal and I try not to let me
} disturb with whatever comes during the study. As researchers we are
} all curious in nature, but I think that we have to try and stay
} focused on our goals. In the end, what matters here is not to
} understand the precise mechanisms of labeling but to get a
} recognition patter of a transcription factor which makes sense in
} the given context.
} In this case, if Robert can show a secondary "unspecific" band for a
} component of desmosomes in western blot, the staining of desmosomes
} in EM is OK, because it cannot interfere with a nuclear pattern
} (provided he has a specific nuclear pattern too). The absence of
} desmosome staining in LM does not need to be explained, it may be
} that upon thawing the antigens lost the conformation which allowed a
} correct immunodetection. Well there are possible explanations for
} the absence of staining in LM, they may remain as hypotheses because
} they do not interfere with the primary goal of location the
} transcription factor.
}
} Finally, you wrote "However, using cells that lack certain
} structures and thus do not show labeling of those structures....
} hmmm...not sure about the relevance of that one!". It IS relevant,
} as long as the-said features are not the object of study. If an
} antibody shows background staining in some cells and not in others,
} why not use the cells with low background staining (provided it
} still makes sense of course)? In the case I provided, I was talking
} about keratinocytes and skin fibroblasts because Robert is working
} with skin. Not because of the absence of desmosomes, but the absence
} of desmosomes is simply an addtional comfort.
}
} Best regards,
} Stéphane
}
}
}
}
} ----- Original Message ----
} From: Jan Leunissen {leunissen-at-aurion.nl}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, June 17, 2009 1:23:31 AM
} Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
}
} Dear Stephane,
}
} Thank you for your appreciation. I do appreciate your slight
} disagreement and would like to add that hopefully from difference of
} opinions there will be in the end a useful contribution to
} explaining the phenomenon that Robert describes.
}
} I think you will agree that researchers have a responsibility to try
} to explain differences that may arise from different approaches. I
} would think it mandatory where the outcome of those approaches
} (being LM and EM immunodetection) are different, but where
} nevertheless an end result is put in the same wording "this or that
} antigen localizes there and there". In Robert's case he has made a
} big effort to keep many of the parameters involved very similar or
} even exactly the same. And exactly that makes the difference that
} much more difficult to understand and thus so interesting and
} challenging. In my opinion the appropriate way forward would be to
} further eliminate the number of remaining procedural parameters in
} an attempt to increase the reliability of the outcome and to
} understand what's going on.
}
} Allow me to comment to a few of the issues you raise.
}
} I did not say antigens are usually less accessible in TEM. I stated
} that 5 and 10 nm gold conjugates do not penetrate readily, even in
} hydrated Tokuyasu sections. The solution is not using higher primary
} concentrations but using a secondary reagent that penetrates more
} easily.
}
} What is the reason for using higher antibody concentrations, if you
} assume antigens are only limitedly accessible, e.g. only at the
} section surface? Those antigens do not "know" they are the only ones
} available for binding. In general, any interaction between those
} antigens and antibodies would only be different if there is a
} difference in avidity binding, or when the antigen was altered to
} affect affinity binding. That does not seem likely in the present
} case, as the material was treated much the same, but ... often there
} are differences without us being aware.
}
} Although maybe not directly relevant for the observations of Robert,
} I do agree that different antibody concentrations may result in
} detecting different antigens/epitopes or molecular structures that
} are very similar in surface structure. Or that the relative signal
} densities may differ. This certainly can be the cause of
} misinterpretations. Antibody concentrations determine what the
} antibodies bind to. This raises the question: which concentration
} (range) is the right one? I wish there was an easy answer, but there
} isn't. In immunochemistry parameters and variables are much more
} easy to establish and understand than in immunohisto- and
} immunocytochemistry. In tissue, after fixation, dehydration,
} freezing, thawing we are likely facing a vast range of modifications
} to the original antigen, both physically and chemically. So being
} consistent, comparing apples with apples, as Leona Cohen-Gould puts
} it, is the way to go.
}
} Strictly, finding a certain antigen pattern in cells does only mean
} that that is the situation for those cells at best, under those
} particular circumstances. Comparing localization in cells with
} tissues should therefore be done with caution, but may certainly
} help. However, using cells that lack certain structures and thus do
} not show labeling of those structures.... hmmm...not sure about the
} relevance of that one!
}
} In my previous message I stated: "...it is very worthwhile to
} understand what goes on. If not, what do we decide is real? The
} label pattern we like, the one that suits us best?" Keeping an open
} mind for what we see, for the results we obtain and making an effort
} to understand those should also discriminate researchers from finders.
}
}
} Cheers,
}
} Jan


==============================Original Headers==============================
13, 20 -- From leunissen-at-aurion.nl Thu Jun 18 00:04:59 2009
13, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5I54w3d024838
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 00:04:59 -0500
13, 20 -- Received: from [192.168.1.50] (really [122.57.245.108]) by fep02.xtra.co.nz
13, 20 -- with ESMTP
13, 20 -- id {20090618050456.GRJH28374.fep02.xtra.co.nz-at-[192.168.1.50]}
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 17:04:56 +1200
13, 20 -- Message-Id: {6E10F8DD-CF6A-47F7-AD54-7AB7A4239458-at-aurion.nl}
13, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
13, 20 -- To: microscopy-at-microscopy.com
13, 20 -- In-Reply-To: {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
13, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
13, 20 -- Date: Thu, 18 Jun 2009 17:04:55 +1200
13, 20 -- References: {200906160904.n5G94H6N029691-at-ns.microscopy.com} {AF3B072D-CB00-442E-9E59-930360ABA76E-at-aurion.nl} {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- X-Mailer: Apple Mail (2.935.3)
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5I54w3d024838
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 18 Jun 2009 03:38:33 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jan!

When you get a nuclear signal in LM and then your recognition pattern is a membrane one in EM, you must admit that something went wrong! If the target is known to be present in the nucleus, you have 99,99% chances that something went wrong with EM. But of course there remains a 0,01% chances that you just discovered something worth a paper in Science :-)
This is what I meant when I was talking about "to make sense". I know that we have to be careful about not being too much influenced by our expectations when analysing our results, but still we have to stay realistic with what we may expect. This is a delicate balance indeed and lots of controls always help.

TEM is definitely no confirmative technique, it is a complementary technique to LM.
Actually TEM is most often used not only to confirm but to increase the precision of a labeling pattern in LM and much less often to deny molecular biology data. In LM you can "see a spot" in the nucleus. In EM, you can say which nuclear structure is involved and even which part of this nuclear structure. It is not a matter of just confirming the LM pattern, it is a very important improvement upon LM results.

Best regards from the sunshiny Vienna, at the roots of the alps and the border of the not-so-blue Danube
And, incidentally, the best city in world to live in!
(see http://www.citymayors.com/features/quality_survey.html)

Stephane

 


----- Original Message ----
X-from: "leunissen-at-aurion.nl" {leunissen-at-aurion.nl}
To: nizets2-at-yahoo.com
Sent: Thursday, June 18, 2009 7:09:14 AM

Dear Stéphane,

This is a very interesting discussion and I hope it will stimulate 
more responses from other members as we go "more fundamental". We are 
perhaps moving away a little from the original thread. However, the 
topic you addressed in the first line of your message challenges me to 
give further explanations.

Even though the difference between limited penetration and limited 
accessibility is fundamental, the end result may be the same and I 
assume that is what you refer to. Both result in low signals, 
sometimes too low to be convincing. To increase labeling efficiency it 
is important to understand the mechanisms. Specimen characteristics 
and specimen preparation control the environment of the antigen. When 
antigens are encapsulated and therefore inaccessible, than that may be 
something one has to accept for instance with antigens in acrylate 
sections. On the other hand sometimes procedures can be applied that 
expose masked antigens, e.g. by etching. Limited penetration on the 
other hand is related to tissue characteristics as well as to the 
reagents, and the development of smaller secondary gold conjugates 
such as Auroprobe One, Undecagold, Nanogold and UltraSmall Gold 
conjugates aimed at increasing labeling efficiency, and successfully so.

The pragmatic approach is a personal choice, certainly, and it is a 
compromise. There may be many reasons why one would choose for a 
pragmatic approach, one being the pressure on research funding. I 
experience that in projects I am involved with too and it does not 
always make me happy to be frank. As is the case with any compromise, 
the chance for a pragmatic approach to be successful depends on how 
well-informed one is about the options and this is where I hope to 
contribute. Personally, whenever possible I choose the way that 
explores more in-depth, in product development for instance. Going in-
depth in one area is probably what allows being pragmatic in another 
area.

When you say "In the end, what matters here is not to understand the 
precise mechanisms of labeling but to get a recognition patter of a 
transcription factor which makes sense in the given context" I have to 
disagree, especially with the second part. This tends to degenerate EM 
to a confirmative technique rather than the unique research technique 
status it deserves when employed properly. If we only look for what we 
or the PI want to see because it fits nicely in existing concepts, 
then we are bound to make only very limited progress, if at all and 
why would we do this in the first place? From my personal experience I 
know many core facilities find themselves confronted with this issue 
and they probably often struggle to get the message across. To me this 
seems a very fundamental issue.
When I was still associated with Utrecht university, longer ago than I 
like to remember, we did some localization work in a collaboration 
project with the microbiology dept. This was in the early days of 
genetic engineering, involving multi-copy gene encoded hybrid proteins 
which were hoped to be excreted or at least to be built into the outer 
membrane of E coli. Biochemical studies using cell fractionation 
indicated the hybrid proteins were in the membrane fraction. 
Microbiologists happy .... and could we please make them a few 
pictures, because it would look nice in the publication! The comic 
book approach. Our localization work with immunogold revealed that the 
hybrid protein was not IN the membrane but stacked below it on the 
cytoplasmic side, blocking export sites etc. It took us a few meetings 
to convey the message, you can probably well imagine. I think this 
illustrates nicely how a confirmative approach would have made us give 
up after a while or would have our immuno EM results to be discarded, 
because the microbiologists thought they did not fit and did not like 
it. Likewise, people coming from a light microscopy immuno background 
often expect EM to give them the same results they obtained in the 
light microscope. Without knowing how those results were obtained and 
what they are worth, that can be hard! And here we are ... back to the 
original topic.

One thing I admit: pragmaticians make for shorter messages!

Best regards everyone, we're heading towards the midwinter solstice 
this weekend in New Zealand with a lantern parade and fireworks and 
then the days will start to lengthen again!! Yay!
Enjoy the longest day and the magical fires on mountain ridges in some 
areas of the Alps in the Northern hemisphere.


Jan


On 17/06/2009, at 8:52 PM, Stephane Nizet wrote:

}
} Hello Jan!
}
} You say that gold conjugates have limited penetration and I say that 
} antigens are less accessible. What is the difference?
} I agree that "increasing the antibody concentration" was a bit 
} hasty. Actually, having a general weaker signal (because of limited 
} penetration), or to say otherwise having less antigens to recognize, 
} you may wish to increase the signal in order to see something. I 
} know that in this regard I have something to learn from you so I 
} will always appreciate your comments on this matter but it all seems 
} to make sense to me.
}
} Now on the necessity to understand what happens: it is all a 
} question of personal goals and priorities I think.
} But I must say that it is my personal experience that it is better 
} to have some limits to explorations. It happened in my career that I 
} spent a lot of time to understand a phenomenon and in the end it did 
} not bring much because that was not what I was looking for. Now I 
} have become more pragmatic: I have a goal and I try not to let me 
} disturb with whatever comes during the study. As researchers we are 
} all curious in nature, but I think that we have to try and stay 
} focused on our goals. In the end, what matters here is not to 
} understand the precise mechanisms of labeling but to get a 
} recognition patter of a transcription factor which makes sense in 
} the given context.
} In this case, if Robert can show a secondary "unspecific" band for a 
} component of desmosomes in western blot, the staining of desmosomes 
} in EM is OK, because it cannot interfere with a nuclear pattern 
} (provided he has a specific nuclear pattern too). The absence of 
} desmosome staining in LM does not need to be explained, it may be 
} that upon thawing the antigens lost the conformation which allowed a 
} correct immunodetection. Well there are possible explanations for 
} the absence of staining in LM, they may remain as hypotheses because 
} they do not interfere with the primary goal of location the 
} transcription factor.
}
} Finally, you wrote "However, using cells that lack certain 
} structures and thus do not show labeling of those structures.... 
} hmmm...not sure about the relevance of that one!". It IS relevant, 
} as long as the-said features are not the object of study. If an 
} antibody shows background staining in some cells and not in others, 
} why not use the cells with low background staining (provided it 
} still makes sense of course)? In the case I provided, I was talking 
} about keratinocytes and skin fibroblasts because Robert is working 
} with skin. Not because of the absence of desmosomes, but the absence 
} of desmosomes is simply an addtional comfort.
}
} Best regards,
} Stéphane
}
}
}
}
} ----- Original Message ----
} From: Jan Leunissen {leunissen-at-aurion.nl}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, June 17, 2009 1:23:31 AM
} Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
}
} Dear Stephane,
}
} Thank you for your appreciation. I do appreciate your slight 
} disagreement and would like to add that hopefully from difference of 
} opinions there will be in the end a useful contribution to 
} explaining the phenomenon that Robert describes.
}
} I think you will agree that researchers have a responsibility to try 
} to explain differences that may arise from different approaches. I 
} would think it mandatory where the outcome of those approaches 
} (being LM and EM immunodetection) are different, but where 
} nevertheless an end result is put in the same wording "this or that 
} antigen localizes there and there". In Robert's case he has made a 
} big effort to keep many of the parameters involved very similar or 
} even exactly the same. And exactly that makes the difference that 
} much more difficult to understand and thus so interesting and 
} challenging. In my opinion the appropriate way forward would be to 
} further eliminate the number of remaining procedural parameters in 
} an attempt to increase the reliability of the outcome and to 
} understand what's going on.
}
} Allow me to comment to a few of the issues you raise.
}
} I did not say antigens are usually less accessible in TEM. I stated 
} that 5 and 10 nm gold conjugates do not penetrate readily, even in 
} hydrated Tokuyasu sections. The solution is not using higher primary 
} concentrations but using a secondary reagent that penetrates more 
} easily.
}
} What is the reason for using higher antibody concentrations, if you 
} assume antigens are only limitedly accessible, e.g. only at the 
} section surface? Those antigens do not "know" they are the only ones 
} available for binding. In general, any interaction between those 
} antigens and antibodies would only be different if there is a 
} difference in avidity binding, or when the antigen was altered to 
} affect affinity binding. That does not seem likely in the present 
} case, as the material was treated much the same, but ... often there 
} are differences without us being aware.
}
} Although maybe not directly relevant for the observations of Robert, 
} I do agree that different antibody concentrations may result in 
} detecting different antigens/epitopes or molecular structures that 
} are very similar in surface structure. Or that the relative signal 
} densities may differ. This certainly can be the cause of 
} misinterpretations. Antibody concentrations determine what the 
} antibodies bind to. This raises the question: which concentration 
} (range) is the right one? I wish there was an easy answer, but there 
} isn't. In immunochemistry parameters and variables are much more 
} easy to establish and understand than in immunohisto- and 
} immunocytochemistry. In tissue, after fixation, dehydration, 
} freezing, thawing we are likely facing a vast range of modifications 
} to the original antigen, both physically and chemically. So being 
} consistent, comparing apples with apples, as Leona Cohen-Gould puts 
} it, is the way to go.
}
} Strictly, finding a certain antigen pattern in cells does only mean 
} that that is the situation for those cells at best, under those 
} particular circumstances. Comparing localization in cells with 
} tissues should therefore be done with caution, but may certainly 
} help. However, using cells that lack certain structures and thus do 
} not show labeling of those structures.... hmmm...not sure about the 
} relevance of that one!
}
} In my previous message I stated: "...it is very worthwhile to 
} understand what goes on. If not, what do we decide is real? The 
} label pattern we like, the one that suits us best?" Keeping an open 
} mind for what we see, for the results we obtain and making an effort 
} to understand those should also discriminate researchers from finders.
}
}
} Cheers,
}
} Jan


==============================Original Headers==============================
13, 20 -- From leunissen-at-aurion.nl Thu Jun 18 00:04:59 2009
13, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
13, 20 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5I54w3d024838
13, 20 --     for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 00:04:59 -0500
13, 20 -- Received: from [192.168.1.50] (really [122.57.245.108]) by fep02.xtra.co.nz
13, 20 --          with ESMTP
13, 20 --          id {20090618050456.GRJH28374.fep02.xtra.co.nz-at-[192.168.1.50]}
13, 20 --          for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 17:04:56 +1200
13, 20 -- Message-Id: {6E10F8DD-CF6A-47F7-AD54-7AB7A4239458-at-aurion.nl}
13, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
13, 20 -- To: microscopy-at-microscopy.com
13, 20 -- In-Reply-To: {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
13, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 20 -- Subject: Re: [Microscopy]  :LM vs TEM immunostain patterns
13, 20 -- Date: Thu, 18 Jun 2009 17:04:55 +1200
13, 20 -- References: {200906160904.n5G94H6N029691-at-ns.microscopy.com} {AF3B072D-CB00-442E-9E59-930360ABA76E-at-aurion.nl} {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- X-Mailer: Apple Mail (2.935.3)
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5I54w3d024838
==============================End of - Headers==============================






==============================Original Headers==============================
31, 25 -- From nizets2-at-yahoo.com Thu Jun 18 03:38:33 2009
31, 25 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
31, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5I8cX5b014744
31, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 03:38:33 -0500
31, 25 -- Received: (qmail 33983 invoked by uid 60001); 18 Jun 2009 08:38:32 -0000
31, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245314312; bh=uGU7yvbfSg3v1O7sTslumbaaKsXX9iBCzLIQWs2ER4Y=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=4N/GK3AAFOKn2BHjEGEvDpiojTtk4lZUFG3XEVsW0NW6nTUaqwAKEJ+FM7QIgZ/yFi4POK0ckusk2spUu2ZPce+lid2CSzV8GGdnVEcVgxQ1ZztRbt3i5wYXZH9Ai/twfCtHwUYC6ruZyaMUNn4PPVOr++nYyNtNQb99T/uh0cY=
31, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
31, 25 -- s=s1024; d=yahoo.com;
31, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
31, 25 -- b=0r4hDtFiYNFI4+YbwMQQpBnvRMG0z2xXEfrF1cbwhEe6Y6741XvMwn/JddFIH7EE7cj4ftQD2V4pgxtPcGVV7eg+/JMF8rJRZXILpBCXQWRAmu1yNPuRk67EJKWtCBmFUOIYt4V5paQEBldUjwDbMVhCOHP32sP79yYThsgB+AU=;
31, 25 -- Message-ID: {684191.33121.qm-at-web110805.mail.gq1.yahoo.com}
31, 25 -- X-YMail-OSG: Wkvs334VM1mdN7VjPyDH1hpKd.hSzwz52rOSjNwLssE7H2JNrUyMVb5uaY2.Wu7Napgkx7yqPfOB7ThWmqPCeXaQGLkNyzBC27XtT4AA9DReSgyleoUd07Tu.mbPufnkp4Jmwg.QqxEkTGhv4IytJPM84_fn2.ccilK7sEsdDHyCjBijCMO7CjUmS57RzsjX03Sog0dJTnc7SdECNuJjR_b1BR9wggortgfMhe7u4tPT9i4ZkhjIthgyy.1IDHNQlWvjZqUO4Owp67Erc5ZB5dECCEoIxJwU26yL6sYopqoeKZAivK9JvMYbVqGeC.nDFHsFyQ2klVDLybigxiRrIlwMB4365rnfVbFSJdHwzoQ-
31, 25 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 18 Jun 2009 01:38:32 PDT
31, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
31, 25 -- References: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- Date: Thu, 18 Jun 2009 01:38:32 -0700 (PDT)
31, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
31, 25 -- Subject: Re: [Microscopy] Re: :LM vs TEM immunostain patterns
31, 25 -- To: leunissen-at-aurion.nl
31, 25 -- Cc: microscopy-at-microscopy.com
31, 25 -- In-Reply-To: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- MIME-Version: 1.0
31, 25 -- Content-Type: text/plain; charset=iso-8859-1
31, 25 -- Content-Transfer-Encoding: 8bit
31, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5I8cX5b014744
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Thu, 18 Jun 2009 09:27:14 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Perhaps we have discovered a new revenue center for research labs- film sets!

I offer (With a wink and apologies) these suggestions for the Fall
2009 prime time lineup:

Johns Hopkins 90210:
A group of young, talented lab techs fall in and out of love over
the years as they wait for bacterial cultures to multiply in their
pursuit of an unknown virus which has been linked with coffee
addiction.

FIB: Miami
Horatio grows impatient as Eric and Ryan troubleshoot vacuum
issues before being able to modify a prototype semiconductor to test
compatibility with a new set of specifications.

NIST:
Gibbs and Denozzo follow leads in the relentless pursuit of
uncalibrated equipment, as Abby finally reveals that she had
contracted out to four other labs, and that is how she was able to get
everything done.

Lab:
Dr. Gregory Lab sees patients who present him with a myriad of
difficult symptoms, prescribes an antibiotic, and the patient gets
better. Lab tries to follow up by obtaining an actual diagnosis, but
ends up getting bogged down with more patients, escapes to the golf
course instead.



My apologies if I have offended anyone, that was not my intention. I
have deepest respect for those of you on the forefront of research. I
just wanted to give you a little chuckle.

--Justin A. Kraft


On Wed, Jun 17, 2009 at 10:39 AM, {Frank_Karl-at-lincolnelectric.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I can't disagree with any of it.  But then,
}
} The public believes:
} That ordinary police discharge their handguns at criminals several times a
} year,
} a person recovers from a gun shot in a less than TV month
} district attorneys try and win 99% of every arrest
} brilliant doctors constantly save every patient while angering patients and
} support staff
} Spies/agents are always good-looking, dashing and resourceful and leave a
} undetected trail of bodies
} WWII prisoners of war constantly got the upper hand on their captors and
} ran resistance cells from the camp
} Bosses are stupid and out witted by carefree employees..
}
} Must I go on....
}
} Entertainment almost always departs from reality and presents a distorted
} view.  If it didn't, it would be a documentary and not what most of us
} would call entertainment.
}
} Can we do anything about it?  Yes, starting with answering questions from
} friends who say..."Did you see...  Can they really do that?"
} Offer to lecture at high Schools, Civic organizations and book clubs.
}
} stay safe.........
}
}
}
}
}
}
}             frah0010-at-umn.edu
}
}             06/17/2009 08:16                                           To
}             AM                        frank_karl-at-lincolnelectric.com
}                                                                        cc
}
}             Please respond to                                     Subject
}             frah0010-at-umn.edu          [Microscopy] Re:  Lab envy?
}
}
}
}
}
}
}
}
}
}
}
}
}
} ----------------------------------------------------------------------------
}
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} gary-at-gaugler.com wrote:
}
} } I don't see why any of us should give any time or effort to this
} } useless venue. . .
}
} One reason we should care about the portrayal of microscopy in films
} and TV (other than simply amusing ourselves) is that it influences how
} the public thinks about microscopy, science, research, etc.  I suspect
} that more people have seen, say, "Spiderman" than have ever seen or
} used a real electron microscope or even had the use and function of
} one explained to them.  Millions of people watch CSI every week, and
} these people are voters, taxpayers, jury members, and consumers of all
} sorts of scientific and medical information, from global warming to
} the H1N1 outbreak.  The portrayal of microscopy and science in popular
} culture is how many people get their information, however right or
} wrong.
}
} If you're looking for how sureality can affect reality, consider the
} so-called CSI Effect:
}
} http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
} http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
} http://www.thepocketpart.org/2006/02/thomas.html
}
} If our clients or students see microscopy and science portrayed badly
} in films and TV, they can carry those expectations into the real
} world.  Many new users of the electron microprobe in my lab expect it
} to operate under what I call the "microwave oven paradigm" -- open the
} door and put in a sample, press one or two settings, hit the "Analyze"
} button, wait a minute or two, and you're done!  I haven't done any
} surveys to learn exactly how they form such misconceptions, but I'd
} hardly be surprised if the portrayal of scientific instruments on TV
} had a lot to do with it.
}
} The point of the discussion isn't really to change Hollywood.  In
} fact, I'd argue that we shouldn't -- even I don't want to watch five
} minutes of doing a sample change in the middle of a film before the
} scientist identifies the mysterious material as otherworldly.
} Instead, we should be aware of the misconceptions that many people
} have and that we need to expel before teaching them to use the
} instrument or handing them their data.  Scientists need to be more
} aware of and involved in the public perception of science.
}
} Best,
} Ellery
}
} -----------------
} Ellery Frahm
} Senior Research Fellow, Department of Geology & Geophysics
} Manager & Principal Analyst, Electron Microprobe Lab
} Doctoral Candidate, Department of Anthropology
} University of Minnesota - Twin Cities campus
} http://umn.edu/~frah0010
}
}
}
}
}
} ==============================Original
} Headers==============================
} 13, 19 -- From frah0010-at-umn.edu Wed Jun 17 07:07:33 2009
} 13, 19 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu
} [134.84.119.32])
} 13, 19 --          by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5HC7VNN015839
} 13, 19 --          for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
} 07:07:31 -0500
} 13, 19 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net
} [75.72.182.206])
} 13, 19 --          by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
} 13, 19 --          for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
} 07:07:31 -0500 (CDT)
} 13, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net
} [75.72.182.206] #+TS+AU+HN
} 13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
} 13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
} 13, 19 -- To: microscopy-at-microscopy.com
} 13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes
} 13, 19 -- Content-Transfer-Encoding: 7bit
} 13, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
} 13, 19 -- Subject: Re: [Microscopy]  Lab envy?
} 13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
} 13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} 13, 19 -- X-Mailer: Apple Mail (2.935.3)
} ==============================End of -
} Headers==============================
}
}
} --
} *************************************************************
} Note:
}  The information contained in this message may be
}  privileged and confidential and protected from disclosure. If
}  the reader of this message is not the intended recipient, or
}  an employee or agent responsible for delivering this message
}  to the intended recipient, you are hereby notified that any
}  dissemination, distribution or copying of this communication
}  is strictly prohibited. If you have received this
}  communication in error, please notify us immediately by
}  replying to the message and deleting it from your computer.
} Thank you,
} The Lincoln Electric Company
} **************************************************************
}
}
} ==============================Original Headers==============================
} 37, 23 -- From frank_karl-at-lincolnelectric.com Wed Jun 17 09:34:31 2009
} 37, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
} 37, 23 --       by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HEYUUt015681
} 37, 23 --       for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 09:34:31 -0500
} 37, 23 -- In-Reply-To: {200906171216.n5HCGEvf028336-at-ns.microscopy.com}
} 37, 23 -- Subject: Lab envy?
} 37, 23 -- Sensitivity:
} 37, 23 -- To: Microscopy-at-microscopy.com
} 37, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
} 37, 23 -- Message-ID: {OFD94177D7.32F79557-ON852575D8.004434AB-852575D8.00500726-at-lincolnelectric.com}
} 37, 23 -- Date: Wed, 17 Jun 2009 10:34:13 -0400
} 37, 23 -- From: Frank_Karl-at-lincolnelectric.com
} 37, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 --       CD-MIME complete at 06/17/2009 10:34:13 AM,
} 37, 23 --       Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 --       Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 --       Serialize complete at 06/17/2009 10:34:13 AM
} 37, 23 -- MIME-Version: 1.0
} 37, 23 -- Content-Type: text/plain;
} 37, 23 --       charset="US-ASCII"
} ==============================End of - Headers==============================
}



--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar


==============================Original Headers==============================
16, 35 -- From kraftpiano-at-gmail.com Thu Jun 18 09:27:13 2009
16, 35 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IERCKB010938
16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 09:27:13 -0500
16, 35 -- Received: by fxm8 with SMTP id 8so1216136fxm.18
16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 07:27:12 -0700 (PDT)
16, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 35 -- d=gmail.com; s=gamma;
16, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
16, 35 -- :date:message-id:subject:from:to:content-type
16, 35 -- :content-transfer-encoding;
16, 35 -- bh=yfEOxXZjFaJVZWpimJ3DTyy4QkaXxp7E8zTwuSF4tyc=;
16, 35 -- b=pjAQ84eqhC8j17eNYNpQuwA4ZWrLRrS6lMo61RHFK38DOOCuj92zExY/ZDf6cxRwjl
16, 35 -- J5hdOMA6/ShdUV4efOJkY9lZ+b9ck9EYhoO+6WT7x9SPPnkeSoJ6LhioOsg9KCpgmf5z
16, 35 -- 2X2G6vNYfCs8zqoBVf8A1Hd48KWAtKGtbaw1M=
16, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
16, 35 -- d=gmail.com; s=gamma;
16, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
16, 35 -- :content-type:content-transfer-encoding;
16, 35 -- b=SgXkMFlx7JRuB68JWqlN1ylqW97RV5Aw8gNQLuhZ+pBnO+PmFkz7QEmXIrSWb9wsL3
16, 35 -- O+X3gE+d3Rsrdr61KHHjBUwQ1qzwgR6Cn7vK2xGZfkkuEDoLN4bsNiayp0/jN6Ag6Vvt
16, 35 -- Jpp5eIGZ7a2P4FRqSPjctTdc8NOgcZB8s5vvk=
16, 35 -- MIME-Version: 1.0
16, 35 -- Received: by 10.204.101.2 with SMTP id a2mr1403069bko.104.1245335231608; Thu,
16, 35 -- 18 Jun 2009 07:27:11 -0700 (PDT)
16, 35 -- In-Reply-To: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
16, 35 -- References: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
16, 35 -- Date: Thu, 18 Jun 2009 10:27:11 -0400
16, 35 -- Message-ID: {25e2b0d20906180727v4e117e2fmc9ad3907075eb4f6-at-mail.gmail.com}
16, 35 -- Subject: Re: [Microscopy] Lab envy?
16, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
16, 35 -- To: Frank_Karl-at-lincolnelectric.com, microscopy-at-microscopy.com
16, 35 -- Content-Type: text/plain; charset=ISO-8859-1
16, 35 -- Content-Transfer-Encoding: 8bit
16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IERCKB010938
==============================End of - Headers==============================




From: underwoo-at-u.washington.edu
Date: Thu, 18 Jun 2009 10:35:17 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,
I appreciate this discussion and agree with both of you. There is a balance to be struck between understanding these types of phenomenon and the pragmatic side of getting the work completed with the best bang for the buck. As usual, in my position, I am caught in the middle. Each time I am confronted with such a phenomenon I am compelled to understand it in order to be confident of the result but mostly to improve the results and interpretation of future experiments. I admit, however, that if I had informed the people who pay my salary how much time and resources has been spent to make each incremental improvement, I would have been fired long ago. I still believe that what they don’t know helps them in the long run and passionately believe that the hypothetical function of a gene product should be supported by morphologic location and the leap to ultrastructural localization is the key to
assigning that function. So having said that, I lie awake at night with fears that a simple component of a cryoprotectant that is so commonly used such as polyvinylpyrrolidone could cause a change in immunolabeling pattern for some proteins. Years of irreplaceable tissue samples are bathed in PVP/sucrose and stored in liquid nitrogen. Just writing that sends -200ºC chills through me. I will continue to pursue it for now and let you know if I find out something relevant.
Thanks for all your suggestions.

Robert Underwood
University of Washington
Dermatology
X-from: nizets2-at-yahoo.com
To: underwoo-at-u.washington.edu


Hi Jan!

When you get a nuclear signal in LM and then your recognition pattern is a
membrane one in EM, you must admit that something went wrong! If the target is
known to be present in the nucleus, you have 99,99% chances that something went
wrong with EM. But of course there remains a 0,01% chances that you just
discovered something worth a paper in Science :-)
This is what I meant when I was talking about "to make sense". I know that we
have to be careful about not being too much influenced by our expectations when
analysing our results, but still we have to stay realistic with what we may
expect. This is a delicate balance indeed and lots of controls always help.

TEM is definitely no confirmative technique, it is a complementary technique to
LM.
Actually TEM is most often used not only to confirm but to increase the
precision of a labeling pattern in LM and much less often to deny molecular
biology data. In LM you can "see a spot" in the nucleus. In EM, you can say
which nuclear structure is involved and even which part of this nuclear
structure. It is not a matter of just confirming the LM pattern, it is a very
important improvement upon LM results.

Best regards from the sunshiny Vienna, at the roots of the alps and the border
of the not-so-blue Danube
And, incidentally, the best city in world to live in!
(see http://www.citymayors.com/features/quality_survey.html)

Stephane




----- Original Message ----
X-from: "leunissen-at-aurion.nl" {leunissen-at-aurion.nl}
To: nizets2-at-yahoo.com
Sent: Thursday, June 18, 2009 7:09:14 AM

Dear Stéphane,

This is a very interesting discussion and I hope it will stimulate
more responses from other members as we go "more fundamental". We are
perhaps moving away a little from the original thread. However, the
topic you addressed in the first line of your message challenges me to
give further explanations.

Even though the difference between limited penetration and limited
accessibility is fundamental, the end result may be the same and I
assume that is what you refer to. Both result in low signals,
sometimes too low to be convincing. To increase labeling efficiency it
is important to understand the mechanisms. Specimen characteristics
and specimen preparation control the environment of the antigen. When
antigens are encapsulated and therefore inaccessible, than that may be
something one has to accept for instance with antigens in acrylate
sections. On the other hand sometimes procedures can be applied that
expose masked antigens, e.g. by etching. Limited penetration on the
other hand is related to tissue characteristics as well as to the
reagents, and the development of smaller secondary gold conjugates
such as Auroprobe One, Undecagold, Nanogold and UltraSmall Gold
conjugates aimed at increasing labeling efficiency, and successfully so.

The pragmatic approach is a personal choice, certainly, and it is a
compromise. There may be many reasons why one would choose for a
pragmatic approach, one being the pressure on research funding. I
experience that in projects I am involved with too and it does not
always make me happy to be frank. As is the case with any compromise,
the chance for a pragmatic approach to be successful depends on how
well-informed one is about the options and this is where I hope to
contribute. Personally, whenever possible I choose the way that
explores more in-depth, in product development for instance. Going in-
depth in one area is probably what allows being pragmatic in another
area.

When you say "In the end, what matters here is not to understand the
precise mechanisms of labeling but to get a recognition patter of a
transcription factor which makes sense in the given context" I have to
disagree, especially with the second part. This tends to degenerate EM
to a confirmative technique rather than the unique research technique
status it deserves when employed properly. If we only look for what we
or the PI want to see because it fits nicely in existing concepts,
then we are bound to make only very limited progress, if at all and
why would we do this in the first place? From my personal experience I
know many core facilities find themselves confronted with this issue
and they probably often struggle to get the message across. To me this
seems a very fundamental issue.
When I was still associated with Utrecht university, longer ago than I
like to remember, we did some localization work in a collaboration
project with the microbiology dept. This was in the early days of
genetic engineering, involving multi-copy gene encoded hybrid proteins
which were hoped to be excreted or at least to be built into the outer
membrane of E coli. Biochemical studies using cell fractionation
indicated the hybrid proteins were in the membrane fraction.
Microbiologists happy .... and could we please make them a few
pictures, because it would look nice in the publication! The comic
book approach. Our localization work with immunogold revealed that the
hybrid protein was not IN the membrane but stacked below it on the
cytoplasmic side, blocking export sites etc. It took us a few meetings
to convey the message, you can probably well imagine. I think this
illustrates nicely how a confirmative approach would have made us give
up after a while or would have our immuno EM results to be discarded,
because the microbiologists thought they did not fit and did not like
it. Likewise, people coming from a light microscopy immuno background
often expect EM to give them the same results they obtained in the
light microscope. Without knowing how those results were obtained and
what they are worth, that can be hard! And here we are ... back to the
original topic.

One thing I admit: pragmaticians make for shorter messages!

Best regards everyone, we're heading towards the midwinter solstice
this weekend in New Zealand with a lantern parade and fireworks and
then the days will start to lengthen again!! Yay!
Enjoy the longest day and the magical fires on mountain ridges in some
areas of the Alps in the Northern hemisphere.


Jan


On 17/06/2009, at 8:52 PM, Stephane Nizet wrote:

}
} Hello Jan!
}
} You say that gold conjugates have limited penetration and I say that
} antigens are less accessible. What is the difference?
} I agree that "increasing the antibody concentration" was a bit
} hasty. Actually, having a general weaker signal (because of limited
} penetration), or to say otherwise having less antigens to recognize,
} you may wish to increase the signal in order to see something. I
} know that in this regard I have something to learn from you so I
} will always appreciate your comments on this matter but it all seems
} to make sense to me.
}
} Now on the necessity to understand what happens: it is all a
} question of personal goals and priorities I think.
} But I must say that it is my personal experience that it is better
} to have some limits to explorations. It happened in my career that I
} spent a lot of time to understand a phenomenon and in the end it did
} not bring much because that was not what I was looking for. Now I
} have become more pragmatic: I have a goal and I try not to let me
} disturb with whatever comes during the study. As researchers we are
} all curious in nature, but I think that we have to try and stay
} focused on our goals. In the end, what matters here is not to
} understand the precise mechanisms of labeling but to get a
} recognition patter of a transcription factor which makes sense in
} the given context.
} In this case, if Robert can show a secondary "unspecific" band for a
} component of desmosomes in western blot, the staining of desmosomes
} in EM is OK, because it cannot interfere with a nuclear pattern
} (provided he has a specific nuclear pattern too). The absence of
} desmosome staining in LM does not need to be explained, it may be
} that upon thawing the antigens lost the conformation which allowed a
} correct immunodetection. Well there are possible explanations for
} the absence of staining in LM, they may remain as hypotheses because
} they do not interfere with the primary goal of location the
} transcription factor.
}
} Finally, you wrote "However, using cells that lack certain
} structures and thus do not show labeling of those structures....
} hmmm...not sure about the relevance of that one!". It IS relevant,
} as long as the-said features are not the object of study. If an
} antibody shows background staining in some cells and not in others,
} why not use the cells with low background staining (provided it
} still makes sense of course)? In the case I provided, I was talking
} about keratinocytes and skin fibroblasts because Robert is working
} with skin. Not because of the absence of desmosomes, but the absence
} of desmosomes is simply an addtional comfort.
}
} Best regards,
} Stéphane
}
}
}
}
} ----- Original Message ----
} From: Jan Leunissen {leunissen-at-aurion.nl}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, June 17, 2009 1:23:31 AM
} Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
}
} Dear Stephane,
}
} Thank you for your appreciation. I do appreciate your slight
} disagreement and would like to add that hopefully from difference of
} opinions there will be in the end a useful contribution to
} explaining the phenomenon that Robert describes.
}
} I think you will agree that researchers have a responsibility to try
} to explain differences that may arise from different approaches. I
} would think it mandatory where the outcome of those approaches
} (being LM and EM immunodetection) are different, but where
} nevertheless an end result is put in the same wording "this or that
} antigen localizes there and there". In Robert's case he has made a
} big effort to keep many of the parameters involved very similar or
} even exactly the same. And exactly that makes the difference that
} much more difficult to understand and thus so interesting and
} challenging. In my opinion the appropriate way forward would be to
} further eliminate the number of remaining procedural parameters in
} an attempt to increase the reliability of the outcome and to
} understand what's going on.
}
} Allow me to comment to a few of the issues you raise.
}
} I did not say antigens are usually less accessible in TEM. I stated
} that 5 and 10 nm gold conjugates do not penetrate readily, even in
} hydrated Tokuyasu sections. The solution is not using higher primary
} concentrations but using a secondary reagent that penetrates more
} easily.
}
} What is the reason for using higher antibody concentrations, if you
} assume antigens are only limitedly accessible, e.g. only at the
} section surface? Those antigens do not "know" they are the only ones
} available for binding. In general, any interaction between those
} antigens and antibodies would only be different if there is a
} difference in avidity binding, or when the antigen was altered to
} affect affinity binding. That does not seem likely in the present
} case, as the material was treated much the same, but ... often there
} are differences without us being aware.
}
} Although maybe not directly relevant for the observations of Robert,
} I do agree that different antibody concentrations may result in
} detecting different antigens/epitopes or molecular structures that
} are very similar in surface structure. Or that the relative signal
} densities may differ. This certainly can be the cause of
} misinterpretations. Antibody concentrations determine what the
} antibodies bind to. This raises the question: which concentration
} (range) is the right one? I wish there was an easy answer, but there
} isn't. In immunochemistry parameters and variables are much more
} easy to establish and understand than in immunohisto- and
} immunocytochemistry. In tissue, after fixation, dehydration,
} freezing, thawing we are likely facing a vast range of modifications
} to the original antigen, both physically and chemically. So being
} consistent, comparing apples with apples, as Leona Cohen-Gould puts
} it, is the way to go.
}
} Strictly, finding a certain antigen pattern in cells does only mean
} that that is the situation for those cells at best, under those
} particular circumstances. Comparing localization in cells with
} tissues should therefore be done with caution, but may certainly
} help. However, using cells that lack certain structures and thus do
} not show labeling of those structures.... hmmm...not sure about the
} relevance of that one!
}
} In my previous message I stated: "...it is very worthwhile to
} understand what goes on. If not, what do we decide is real? The
} label pattern we like, the one that suits us best?" Keeping an open
} mind for what we see, for the results we obtain and making an effort
} to understand those should also discriminate researchers from finders.
}
}
} Cheers,
}
} Jan


==============================Original Headers==============================
13, 20 -- From leunissen-at-aurion.nl Thu Jun 18 00:04:59 2009
13, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5I54w3d024838
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 00:04:59 -0500
13, 20 -- Received: from [192.168.1.50] (really [122.57.245.108]) by
fep02.xtra.co.nz
13, 20 -- with ESMTP
13, 20 -- id {20090618050456.GRJH28374.fep02.xtra.co.nz-at-[192.168.1.50]}
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 17:04:56
+1200
13, 20 -- Message-Id: {6E10F8DD-CF6A-47F7-AD54-7AB7A4239458-at-aurion.nl}
13, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
13, 20 -- To: microscopy-at-microscopy.com
13, 20 -- In-Reply-To: {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
13, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
13, 20 -- Date: Thu, 18 Jun 2009 17:04:55 +1200
13, 20 -- References: {200906160904.n5G94H6N029691-at-ns.microscopy.com}
{AF3B072D-CB00-442E-9E59-930360ABA76E-at-aurion.nl}
{609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- X-Mailer: Apple Mail (2.935.3)
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n5I54w3d024838
==============================End of - Headers==============================






==============================Original Headers==============================
31, 25 -- From nizets2-at-yahoo.com Thu Jun 18 03:38:33 2009
31, 25 -- Received: from web110805.mail.gq1.yahoo.com
(web110805.mail.gq1.yahoo.com [67.195.13.228])
31, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n5I8cX5b014744
31, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 03:38:33 -0500
31, 25 -- Received: (qmail 33983 invoked by uid 60001); 18 Jun 2009 08:38:32
-0000
31, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1245314312; bh=uGU7yvbfSg3v1O7sTslumbaaKsXX9iBCzLIQWs2ER4Y=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In
-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=4N/GK3AAFOKn2BHjEGEvDpiojTtk4lZUFG3XEVsW0NW6nTUaqwAKEJ+FM7QIgZ/yFi4POK0ckusk2s
pUu2ZPce+lid2CSzV8GGdnVEcVgxQ1ZztRbt3i5wYXZH9Ai/twfCtHwUYC6ruZyaMUNn4PPVOr++nYyN
tNQb99T/uh0cY=
31, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
31, 25 -- s=s1024; d=yahoo.com;
31, 25 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In
-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
31, 25 --
b=0r4hDtFiYNFI4+YbwMQQpBnvRMG0z2xXEfrF1cbwhEe6Y6741XvMwn/JddFIH7EE7cj4ftQD2V4pgx
tPcGVV7eg+/JMF8rJRZXILpBCXQWRAmu1yNPuRk67EJKWtCBmFUOIYt4V5paQEBldUjwDbMVhCOHP32s
P79yYThsgB+AU=;
31, 25 -- Message-ID: {684191.33121.qm-at-web110805.mail.gq1.yahoo.com}
31, 25 -- X-YMail-OSG:
Wkvs334VM1mdN7VjPyDH1hpKd.hSzwz52rOSjNwLssE7H2JNrUyMVb5uaY2.Wu7Napgkx7yqPfOB7ThW
mqPCeXaQGLkNyzBC27XtT4AA9DReSgyleoUd07Tu.mbPufnkp4Jmwg.QqxEkTGhv4IytJPM84_fn2.cc
ilK7sEsdDHyCjBijCMO7CjUmS57RzsjX03Sog0dJTnc7SdECNuJjR_b1BR9wggortgfMhe7u4tPT9i4Z
khjIthgyy.1IDHNQlWvjZqUO4Owp67Erc5ZB5dECCEoIxJwU26yL6sYopqoeKZAivK9JvMYbVqGeC.nD
FHsFyQ2klVDLybigxiRrIlwMB4365rnfVbFSJdHwzoQ-
31, 25 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via
HTTP; Thu, 18 Jun 2009 01:38:32 PDT
31, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
31, 25 -- References: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- Date: Thu, 18 Jun 2009 01:38:32 -0700 (PDT)
31, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
31, 25 -- Subject: Re: [Microscopy] Re: :LM vs TEM immunostain patterns
31, 25 -- To: leunissen-at-aurion.nl
31, 25 -- Cc: microscopy-at-microscopy.com
31, 25 -- In-Reply-To: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- MIME-Version: 1.0
31, 25 -- Content-Type: text/plain; charset=iso-8859-1
31, 25 -- Content-Transfer-Encoding: 8bit
31, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n5I8cX5b014744
==============================End of - Headers==============================








==============================Original Headers==============================
47, 24 -- From underwoo-at-u.washington.edu Thu Jun 18 10:35:17 2009
47, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134])
47, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IFZG1V027383
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 10:35:17 -0500
47, 24 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103])
47, 24 -- by mxout1.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5IFZFad018188
47, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 08:35:15 -0700
47, 24 -- Received: from localhost (localhost [127.0.0.1])
47, 24 -- by hymn13.u.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) with ESMTP id n5IFZCm4005460
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 08:35:12 -0700
47, 24 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Thu, 18 Jun 2009 08:35:12 PDT
47, 24 -- Date: Thu, 18 Jun 2009 08:35:12 -0700 (PDT)
47, 24 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
47, 24 -- To: Microscopy List {Microscopy-at-microscopy.Com}
47, 24 -- Subject: [Microscopy]: LM vs TEM immuostain pattern changes
47, 24 -- Message-ID: {Pine.LNX.4.43.0906180835120.25964-at-hymn13.u.washington.edu}
47, 24 -- MIME-Version: 1.0
47, 24 -- Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed
47, 24 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.6.18.152115
47, 24 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
47, 24 -- SUPERLONG_LINE 0.05, BODY_SIZE_10000_PLUS 0, ECARD_KNOWN_DOMAINS 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
47, 24 -- Content-Transfer-Encoding: 8bit
47, 24 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n5IFZG1V027383
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 18 Jun 2009 11:55:36 -0500
Subject: [Microscopy] Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Justin!

Those were well thought out shows, now we just need to get someone to pitch them to the networks.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.


--- On Thu, 6/18/09, kraftpiano-at-gmail.com {kraftpiano-at-gmail.com} wrote:

} From: kraftpiano-at-gmail.com {kraftpiano-at-gmail.com}
} Subject: [Microscopy] Re: Lab envy?
} To: vapatpxs-at-yahoo.com
} Date: Thursday, June 18, 2009, 2:34 PM
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Perhaps we have discovered a new revenue center for
} research labs- film sets!
}
} I offer (With a wink and apologies) these suggestions for
} the Fall
} 2009 prime time lineup:
}
} Johns Hopkins 90210:
}      A group of young, talented lab
} techs fall in and out of love over
} the years as they wait for bacterial cultures to multiply
} in their
} pursuit of an unknown virus which has been linked with
} coffee
} addiction.
}
} FIB: Miami
}      Horatio grows impatient as Eric
} and Ryan troubleshoot vacuum
} issues before being able to modify a prototype
} semiconductor to test
} compatibility with a new set of specifications.
}
} NIST:
}      Gibbs and Denozzo follow leads in
} the relentless pursuit of
} uncalibrated equipment, as Abby finally reveals that she
} had
} contracted out to four other labs, and that is how she was
} able to get
} everything done.
}
} Lab:
}       Dr. Gregory Lab sees patients who
} present him with a myriad of
} difficult symptoms, prescribes an antibiotic, and the
} patient gets
} better.  Lab tries to follow up by obtaining an actual
} diagnosis, but
} ends up getting bogged down with more patients, escapes to
} the golf
} course instead.
}
}
}
} My apologies if I have offended anyone, that was not my
} intention.  I
} have deepest respect for those of you on the forefront of
} research.  I
} just wanted to give you a little chuckle.
}
} --Justin A. Kraft
}
}
} On Wed, Jun 17, 2009 at 10:39 AM, {Frank_Karl-at-lincolnelectric.com}
} wrote:
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } I can't disagree with any of it.  But then,
} }
} } The public believes:
} } That ordinary police discharge their handguns at
} criminals several times a
} } year,
} } a person recovers from a gun shot in a less than TV
} month
} } district attorneys try and win 99% of every arrest
} } brilliant doctors constantly save every patient while
} angering patients and
} } support staff
} } Spies/agents are always good-looking, dashing and
} resourceful and leave a
} } undetected trail of bodies
} } WWII prisoners of war constantly got the upper hand on
} their captors and
} } ran resistance cells from the camp
} } Bosses are stupid and out witted by carefree
} employees..
} }
} } Must I go on....
} }
} } Entertainment almost always departs from reality and
} presents a distorted
} } view.  If it didn't, it would be a documentary and
} not what most of us
} } would call entertainment.
} }
} } Can we do anything about it?  Yes, starting with
} answering questions from
} } friends who say..."Did you see...  Can they really do
} that?"
} } Offer to lecture at high Schools, Civic organizations
} and book clubs.
} }
} } stay safe.........
} }
} }
} }
} }
} }
} }
} }             frah0010-at-umn.edu
} }
} }             06/17/2009 08:16            
}                               To
} }             AM                      
}  frank_karl-at-lincolnelectric.com
} }                                    
}                                    cc
} }
} }             Please respond to            
}                         Subject
} }             frah0010-at-umn.edu
}          [Microscopy] Re:  Lab envy?
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} }
} } gary-at-gaugler.com
} wrote:
} }
} } } I don't see why any of us should give any time or
} effort to this
} } } useless venue. . .
} }
} } One reason we should care about the portrayal of
} microscopy in films
} } and TV (other than simply amusing ourselves) is that
} it influences how
} } the public thinks about microscopy, science, research,
} etc.  I suspect
} } that more people have seen, say, "Spiderman" than have
} ever seen or
} } used a real electron microscope or even had the use
} and function of
} } one explained to them.  Millions of people watch CSI
} every week, and
} } these people are voters, taxpayers, jury members, and
} consumers of all
} } sorts of scientific and medical information, from
} global warming to
} } the H1N1 outbreak.  The portrayal of microscopy and
} science in popular
} } culture is how many people get their information,
} however right or
} } wrong.
} }
} } If you're looking for how sureality can affect
} reality, consider the
} } so-called CSI Effect:
} }
} } http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
} } http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
} } http://www.thepocketpart.org/2006/02/thomas.html
} }
} } If our clients or students see microscopy and science
} portrayed badly
} } in films and TV, they can carry those expectations
} into the real
} } world.  Many new users of the electron microprobe in
} my lab expect it
} } to operate under what I call the "microwave oven
} paradigm" -- open the
} } door and put in a sample, press one or two settings,
} hit the "Analyze"
} } button, wait a minute or two, and you're done!  I
} haven't done any
} } surveys to learn exactly how they form such
} misconceptions, but I'd
} } hardly be surprised if the portrayal of scientific
} instruments on TV
} } had a lot to do with it.
} }
} } The point of the discussion isn't really to change
} Hollywood.  In
} } fact, I'd argue that we shouldn't -- even I don't want
} to watch five
} } minutes of doing a sample change in the middle of a
} film before the
} } scientist identifies the mysterious material as
} otherworldly.
} } Instead, we should be aware of the misconceptions that
} many people
} } have and that we need to expel before teaching them to
} use the
} } instrument or handing them their data.  Scientists
} need to be more
} } aware of and involved in the public perception of
} science.
} }
} } Best,
} } Ellery
} }
} } -----------------
} } Ellery Frahm
} } Senior Research Fellow, Department of Geology &
} Geophysics
} } Manager & Principal Analyst, Electron Microprobe
} Lab
} } Doctoral Candidate, Department of Anthropology
} } University of Minnesota - Twin Cities campus
} } http://umn.edu/~frah0010
} }
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 13, 19 -- From frah0010-at-umn.edu
} Wed Jun 17 07:07:33 2009
} } 13, 19 -- Received: from mta-w3.tc.umn.edu
} (mta-w3.tc.umn.edu
} } [134.84.119.32])
} } 13, 19 --          by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with
} } ESMTP id n5HC7VNN015839
} } 13, 19 --          for {microscopy-at-microscopy.com} ;
} Wed, 17 Jun 2009
} } 07:07:31 -0500
} } 13, 19 -- Received: from [10.0.1.199]
} (c-75-72-182-206.hsd1.mn.comcast.net
} } [75.72.182.206])
} } 13, 19 --          by mta-w3.tc.umn.edu (UMN
} smtpd) with ESMTP
} } 13, 19 --          for {microscopy-at-microscopy.com} ;
} Wed, 17 Jun 2009
} } 07:07:31 -0500 (CDT)
} } 13, 19 -- X-Umn-Remote-Mta: [N]
} c-75-72-182-206.hsd1.mn.comcast.net
} } [75.72.182.206] #+TS+AU+HN
} } 13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
} } 13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
} } 13, 19 -- To: microscopy-at-microscopy.com
} } 13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} } 13, 19 -- Content-Type: text/plain; charset=US-ASCII;
} format=flowed;
} } delsp=yes
} } 13, 19 -- Content-Transfer-Encoding: 7bit
} } 13, 19 -- Mime-Version: 1.0 (Apple Message framework
} v935.3)
} } 13, 19 -- Subject: Re: [Microscopy]  Lab envy?
} } 13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
} } 13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} } 13, 19 -- X-Mailer: Apple Mail (2.935.3)
} } ==============================End of -
} } Headers==============================
} }
} }
} } --
} }
} *************************************************************
} } Note:
} }  The information contained in this message may be
} }  privileged and confidential and protected from
} disclosure. If
} }  the reader of this message is not the intended
} recipient, or
} }  an employee or agent responsible for delivering this
} message
} }  to the intended recipient, you are hereby notified
} that any
} }  dissemination, distribution or copying of this
} communication
} }  is strictly prohibited. If you have received this
} }  communication in error, please notify us immediately
} by
} }  replying to the message and deleting it from your
} computer.
} } Thank you,
} } The Lincoln Electric Company
} }
} **************************************************************
} }
} }
} } ==============================Original
} Headers==============================
} } 37, 23 -- From frank_karl-at-lincolnelectric.com
} Wed Jun 17 09:34:31 2009
} } 37, 23 -- Received: from lincolnelectric.com
} (smtp2.lincolnelectric.com [64.109.211.115])
} } 37, 23 --       by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n5HEYUUt015681
} } 37, 23 --       for {microscopy-at-microscopy.com} ;
} Wed, 17 Jun 2009 09:34:31 -0500
} } 37, 23 -- In-Reply-To: {200906171216.n5HCGEvf028336-at-ns.microscopy.com}
} } 37, 23 -- Subject: Lab envy?
} } 37, 23 -- Sensitivity:
} } 37, 23 -- To: Microscopy-at-microscopy.com
} } 37, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November
} 30, 2005
} } 37, 23 -- Message-ID: {OFD94177D7.32F79557-ON852575D8.004434AB-852575D8.00500726-at-lincolnelectric.com}
} } 37, 23 -- Date: Wed, 17 Jun 2009 10:34:13 -0400
} } 37, 23 -- From: Frank_Karl-at-lincolnelectric.com
} } 37, 23 -- X-MIMETrack: CD-MIME by Router on
} Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} } 37, 23 --       CD-MIME complete at 06/17/2009
} 10:34:13 AM,
} } 37, 23 --       Itemize by Router on
} Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} } 37, 23 --       Serialize by Router on
} Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } 37, 23 --  07, 2008) at 06/17/2009 10:34:13 AM,
} } 37, 23 --       Serialize complete at 06/17/2009
} 10:34:13 AM
} } 37, 23 -- MIME-Version: 1.0
} } 37, 23 -- Content-Type: text/plain;
} } 37, 23 --       charset="US-ASCII"
} } ==============================End of -
} Headers==============================
} }
}
}
}
} --
} "America believes in education; the average professor earns
} more money
} in a year than a professional athlete earns in a whole
} week." Evan
} Esar
}
}
} ==============================Original
} Headers==============================
} 16, 35 -- From kraftpiano-at-gmail.com
} Thu Jun 18 09:27:13 2009
} 16, 35 -- Received: from mail-fx0-f212.google.com
} (mail-fx0-f212.google.com [209.85.220.212])
} 16, 35 --     by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n5IERCKB010938
} 16, 35 --     for {microscopy-at-microscopy.com} ;
} Thu, 18 Jun 2009 09:27:13 -0500
} 16, 35 -- Received: by fxm8 with SMTP id 8so1216136fxm.18
} 16, 35 --         for {microscopy-at-microscopy.com} ;
} Thu, 18 Jun 2009 07:27:12 -0700 (PDT)
} 16, 35 -- DKIM-Signature: v=1; a=rsa-sha256;
} c=relaxed/relaxed;
} 16, 35 --     
}    d=gmail.com; s=gamma;
} 16, 35 --     
}    h=domainkey-signature:mime-version:received:in-reply-to:references
} 16, 35 --         
} :date:message-id:subject:from:to:content-type
} 16, 35 --         
} :content-transfer-encoding;
} 16, 35 --     
}    bh=yfEOxXZjFaJVZWpimJ3DTyy4QkaXxp7E8zTwuSF4tyc=;
} 16, 35 --     
}    b=pjAQ84eqhC8j17eNYNpQuwA4ZWrLRrS6lMo61RHFK38DOOCuj92zExY/ZDf6cxRwjl
} 16, 35 --         
} J5hdOMA6/ShdUV4efOJkY9lZ+b9ck9EYhoO+6WT7x9SPPnkeSoJ6LhioOsg9KCpgmf5z
} 16, 35 --         
} 2X2G6vNYfCs8zqoBVf8A1Hd48KWAtKGtbaw1M=
} 16, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 16, 35 --     
}    d=gmail.com; s=gamma;
} 16, 35 --     
}    h=mime-version:in-reply-to:references:date:message-id:subject:from:to
} 16, 35 --         
} :content-type:content-transfer-encoding;
} 16, 35 --     
}    b=SgXkMFlx7JRuB68JWqlN1ylqW97RV5Aw8gNQLuhZ+pBnO+PmFkz7QEmXIrSWb9wsL3
} 16, 35 --         
} O+X3gE+d3Rsrdr61KHHjBUwQ1qzwgR6Cn7vK2xGZfkkuEDoLN4bsNiayp0/jN6Ag6Vvt
} 16, 35 --         
} Jpp5eIGZ7a2P4FRqSPjctTdc8NOgcZB8s5vvk=
} 16, 35 -- MIME-Version: 1.0
} 16, 35 -- Received: by 10.204.101.2 with SMTP id
} a2mr1403069bko.104.1245335231608; Thu,
} 16, 35 --     18 Jun 2009 07:27:11 -0700
} (PDT)
} 16, 35 -- In-Reply-To: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
} 16, 35 -- References: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
} 16, 35 -- Date: Thu, 18 Jun 2009 10:27:11 -0400
} 16, 35 -- Message-ID: {25e2b0d20906180727v4e117e2fmc9ad3907075eb4f6-at-mail.gmail.com}
} 16, 35 -- Subject: Re: [Microscopy] Lab envy?
} 16, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} 16, 35 -- To: Frank_Karl-at-lincolnelectric.com,
} microscopy-at-microscopy.com
} 16, 35 -- Content-Type: text/plain; charset=ISO-8859-1
} 16, 35 -- Content-Transfer-Encoding: 8bit
} 16, 35 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n5IERCKB010938
} ==============================End of -
} Headers==============================
}





==============================Original Headers==============================
12, 22 -- From vapatpxs-at-yahoo.com Thu Jun 18 11:55:35 2009
12, 22 -- Received: from web46114.mail.sp1.yahoo.com (web46114.mail.sp1.yahoo.com [68.180.199.131])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5IGtZTO013213
12, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 18 Jun 2009 11:55:35 -0500
12, 22 -- Received: (qmail 95991 invoked by uid 60001); 18 Jun 2009 16:55:33 -0000
12, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245344132; bh=fP+FEljZC8gqyX+LReJdb9V2qNJLO+S1c6/yskIo8/0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=tvS2IrrG+XW7z9YDmMfGPp360FpHFsT8vhS3nm2QPmqqOs5RG9AXysQ84qkoT5JUPGBEBbwMJL2lzFFH6YvuxRP/qFuOAiYR7Ol7p5YeOfVKLs/P+6ocspS869sG1yxPxL7CNG6//YG+z3TgoIYzoOCAHc9frShLUOlmxB+E9Cw=
12, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 22 -- s=s1024; d=yahoo.com;
12, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
12, 22 -- b=bIDaHugfDxHI0Dx+HFiFkBop2SZDiw9QZg/hOBNRh1xqT1KZHkrFfImd6ALgSSOZYTjKFydtEJO6Ov2INMP6jJPYmjFeU3LsmmMBbrLTcrgf5KcaTu//NYIls/8GhnZ3h/CIX2xbKcx9cUuxVayaB9Y5dg6/TnjCo4GPLqJeGBI=;
12, 22 -- Message-ID: {518693.95135.qm-at-web46114.mail.sp1.yahoo.com}
12, 22 -- X-YMail-OSG: jEq7qqAVM1m3X9z8yc0.MViPOx7qjRweq4ti1vdIxy2xOeYBToETPeOGPSZvSySPhKUrdJ65Y9gtPwIDCpw9gkrXxvCq3wiiMwm6lhz5ez.9eNeZ1isOvIn.vSPeWP.L6x1m5knvOJb8Rmteba6cSfXyT5MoT8UQJJNmvP3NMYin0AaSaCjkSYYSF3XptgH3H0EEhsIj7_Zy5qR2vAdurBD8KDLdMqsz3zhMSge3m3.uvomrEdcpi5YarP15LsWV7hyTZfFlK.Fk0uKU_yh4VGePG7X1DzrYgZOYIz6.sFOLrrKLwDgrpduC9U7VirrQTGUBvoeiXGHnXE5v1OzpSAW2N9qjSOuqmtjN4IqczZM-
12, 22 -- Received: from [132.239.85.200] by web46114.mail.sp1.yahoo.com via HTTP; Thu, 18 Jun 2009 09:55:32 PDT
12, 22 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
12, 22 -- Date: Thu, 18 Jun 2009 09:55:32 -0700 (PDT)
12, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 22 -- Subject: Re: [Microscopy] Re: Lab envy?
12, 22 -- To: kraftpiano-at-gmail.com, MSA BB {Microscopy-at-Microscopy.Com}
12, 22 -- MIME-Version: 1.0
12, 22 -- Content-Type: text/plain; charset=iso-8859-1
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IGtZTO013213
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Thu, 18 Jun 2009 13:19:32 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Billy Mays.
"But wait! There's more! Order "Lab" now, and
we'll throw in 'As the Centrifuge Turns' at no
additional cost*!"

*just pay shipping and handling (but we're not
telling you that's 2X the cost of the show)

Phil

} Thanks Justin!
}
} Those were well thought out shows, now we just
} need to get someone to pitch them to the
} networks.
}
} Paula :-)
}
} Paula Sicurello
} VA Medical Center San Diego
} Veterans Medical Research Foundation (VMRF)
} Core Research Imaging Center (CRIC)
} 3350 La Jolla Village Dr., MC151
} San Diego, CA 92161
} 858-552-8585 x2397
}
} Your images flow through our CRIC.
}
}
} --- On Thu, 6/18/09, kraftpiano-at-gmail.com {kraftpiano-at-gmail.com} wrote:
}
} } From: kraftpiano-at-gmail.com {kraftpiano-at-gmail.com}
} } Subject: [Microscopy] Re: Lab envy?
} } To: vapatpxs-at-yahoo.com
} } Date: Thursday, June 18, 2009, 2:34 PM
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:Ý The
} } Microscopy Society of America
} } ToÝ Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Perhaps we have discovered a new revenue center for
} } research labs- film sets!
} }
} } I offer (With a wink and apologies) these suggestions for
} } the Fall
} } 2009 prime time lineup:
} }
} } Johns Hopkins 90210:
} } Ý ÝÝÝA group of young, talented lab
} } techs fall in and out of love over
} } the years as they wait for bacterial cultures to multiply
} } in their
} } pursuit of an unknown virus which has been linked with
} } coffee
} } addiction.
} }
} } FIB: Miami
} } Ý ÝÝÝHoratio grows impatient as Eric
} } and Ryan troubleshoot vacuum
} } issues before being able to modify a prototype
} } semiconductor to test
} } compatibility with a new set of specifications.
} }
} } NIST:
} } Ý ÝÝÝGibbs and Denozzo follow leads in
} } the relentless pursuit of
} } uncalibrated equipment, as Abby finally reveals that she
} } had
} } contracted out to four other labs, and that is how she was
} } able to get
} } everything done.
} }
} } Lab:
} } Ý Ý Ý Dr. Gregory Lab sees patients who
} } present him with a myriad of
} } difficult symptoms, prescribes an antibiotic, and the
} } patient gets
} } better.Ý Lab tries to follow up by obtaining an actual
} } diagnosis, but
} } ends up getting bogged down with more patients, escapes to
} } the golf
} } course instead.
} }
} }
} }
} } My apologies if I have offended anyone, that was not my
} } intention.Ý I
} } have deepest respect for those of you on the forefront of
} } research.Ý I
} } just wanted to give you a little chuckle.
} }
} } --Justin A. Kraft
} }
} }
} } On Wed, Jun 17, 2009 at 10:39 AM, {Frank_Karl-at-lincolnelectric.com}
} } wrote:
} } }
} } }
} } }
} } }
} } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: ÝThe
} } Microscopy Society of America
} } } To ÝSubscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ----------------------------------------------------------------------------
} } }
} } } I can't disagree with any of it. ÝBut then,
} } }
} } } The public believes:
} } } That ordinary police discharge their handguns at
} } criminals several times a
} } } year,
} } } a person recovers from a gun shot in a less than TV
} } month
} } } district attorneys try and win 99% of every arrest
} } } brilliant doctors constantly save every patient while
} } angering patients and
} } } support staff
} } } Spies/agents are always good-looking, dashing and
} } resourceful and leave a
} } } undetected trail of bodies
} } } WWII prisoners of war constantly got the upper hand on
} } their captors and
} } } ran resistance cells from the camp
} } } Bosses are stupid and out witted by carefree
} } employees..
} } }
} } } Must I go on....
} } }
} } } Entertainment almost always departs from reality and
} } presents a distorted
} } } view. ÝIf it didn't, it would be a documentary and
} } not what most of us
} } } would call entertainment.
} } }
} } } Can we do anything about it? ÝYes, starting with
} } answering questions from
} } } friends who say..."Did you see... ÝCan they really do
} } that?"
} } } Offer to lecture at high Schools, Civic organizations
} } and book clubs.
} } }
} } } stay safe.........
} } }
} } }
} } }
} } }
} } }
} } }
} } } Ý Ý Ý Ý Ý Ý frah0010-at-umn.edu
} } }
} } } Ý Ý Ý Ý Ý Ý 06/17/2009 08:16 Ý Ý Ý Ý Ý Ý
} } Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý To
} } } Ý Ý Ý Ý Ý Ý AM Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý
} } Ýfrank_karl-at-lincolnelectric.com
} } } Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý
} } Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ýcc
} } }
} } } Ý Ý Ý Ý Ý Ý Please respond to Ý Ý Ý Ý Ý Ý
} } Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Ý Subject
} } } Ý Ý Ý Ý Ý Ý frah0010-at-umn.edu
} } Ý Ý Ý Ý Ý[Microscopy] Re: ÝLab envy?
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } ----------------------------------------------------------------------------
} } }
} } } The Microscopy ListServer -- CoSponsor: ÝThe
} } Microscopy Society of America
} } } To ÝSubscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ----------------------------------------------------------------------------
} } }
} } }
} } } gary-at-gaugler.com
} } wrote:
} } }
} } } } I don't see why any of us should give any time or
} } effort to this
} } } } useless venue. . .
} } }
} } } One reason we should care about the portrayal of
} } microscopy in films
} } } and TV (other than simply amusing ourselves) is that
} } it influences how
} } } the public thinks about microscopy, science, research,
} } etc. ÝI suspect
} } } that more people have seen, say, "Spiderman" than have
} } ever seen or
} } } used a real electron microscope or even had the use
} } and function of
} } } one explained to them. ÝMillions of people watch CSI
} } every week, and
} } } these people are voters, taxpayers, jury members, and
} } consumers of all
} } } sorts of scientific and medical information, from
} } global warming to
} } } the H1N1 outbreak. ÝThe portrayal of microscopy and
} } science in popular
} } } culture is how many people get their information,
} } however right or
} } } wrong.
} } }
} } } If you're looking for how sureality can affect
} } reality, consider the
} } } so-called CSI Effect:
} } }
} } } http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
} } } http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
} } } http://www.thepocketpart.org/2006/02/thomas.html
} } }
} } } If our clients or students see microscopy and science
} } portrayed badly
} } } in films and TV, they can carry those expectations
} } into the real
} } } world. ÝMany new users of the electron microprobe in
} } my lab expect it
} } } to operate under what I call the "microwave oven
} } paradigm" -- open the
} } } door and put in a sample, press one or two settings,
} } hit the "Analyze"
} } } button, wait a minute or two, and you're done! ÝI
} } haven't done any
} } } surveys to learn exactly how they form such
} } misconceptions, but I'd
} } } hardly be surprised if the portrayal of scientific
} } instruments on TV
} } } had a lot to do with it.
} } }
} } } The point of the discussion isn't really to change
} } Hollywood. ÝIn
} } } fact, I'd argue that we shouldn't -- even I don't want
} } to watch five
} } } minutes of doing a sample change in the middle of a
} } film before the
} } } scientist identifies the mysterious material as
} } otherworldly.
} } } Instead, we should be aware of the misconceptions that
} } many people
} } } have and that we need to expel before teaching them to
} } use the
} } } instrument or handing them their data. ÝScientists
} } need to be more
} } } aware of and involved in the public perception of
} } science.
} } }
} } } Best,
} } } Ellery
} } }
} } } -----------------
} } } Ellery Frahm
} } } Senior Research Fellow, Department of Geology &
} } Geophysics
} } } Manager & Principal Analyst, Electron Microprobe
} } Lab
} } } Doctoral Candidate, Department of Anthropology
} } } University of Minnesota - Twin Cities campus
} } } http://umn.edu/~frah0010
} } }
} } }
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 13, 19 -- From frah0010-at-umn.edu
} } Wed Jun 17 07:07:33 2009
} } } 13, 19 -- Received: from mta-w3.tc.umn.edu
} } (mta-w3.tc.umn.edu
} } } [134.84.119.32])
} } } 13, 19 -- Ý Ý Ý Ý Ýby ns.microscopy.com
} } (8.12.11.20060308/8.12.8) with
} } } ESMTP id n5HC7VNN015839
} } } 13, 19 -- Ý Ý Ý Ý Ýfor {microscopy-at-microscopy.com} ;
} } Wed, 17 Jun 2009
} } } 07:07:31 -0500
} } } 13, 19 -- Received: from [10.0.1.199]
} } (c-75-72-182-206.hsd1.mn.comcast.net
} } } [75.72.182.206])
} } } 13, 19 -- Ý Ý Ý Ý Ýby mta-w3.tc.umn.edu (UMN
} } smtpd) with ESMTP
} } } 13, 19 -- Ý Ý Ý Ý Ýfor {microscopy-at-microscopy.com} ;
} } Wed, 17 Jun 2009
} } } 07:07:31 -0500 (CDT)
} } } 13, 19 -- X-Umn-Remote-Mta: [N]
} } c-75-72-182-206.hsd1.mn.comcast.net
} } } [75.72.182.206] #+TS+AU+HN
} } } 13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
} } } 13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
} } } 13, 19 -- To: microscopy-at-microscopy.com
} } } 13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} } } 13, 19 -- Content-Type: text/plain; charset=US-ASCII;
} } format=flowed;
} } } delsp=yes
} } } 13, 19 -- Content-Transfer-Encoding: 7bit
} } } 13, 19 -- Mime-Version: 1.0 (Apple Message framework
} } v935.3)
} } } 13, 19 -- Subject: Re: [Microscopy] ÝLab envy?
} } } 13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
} } } 13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} } } 13, 19 -- X-Mailer: Apple Mail (2.935.3)
} } } ==============================End of -
} } } Headers==============================
} } }
} } }
} } } --
} } }
} } *************************************************************
} } } Note:
} } } ÝThe information contained in this message may be
} } } Ýprivileged and confidential and protected from
} } disclosure. If
} } } Ýthe reader of this message is not the intended
} } recipient, or
} } } Ýan employee or agent responsible for delivering this
} } message
} } } Ýto the intended recipient, you are hereby notified
} } that any
} } } Ýdissemination, distribution or copying of this
} } communication
} } } Ýis strictly prohibited. If you have received this
} } } Ýcommunication in error, please notify us immediately
} } by
} } } Ýreplying to the message and deleting it from your
} } computer.
} } } Thank you,
} } } The Lincoln Electric Company
} } }
} } **************************************************************
} } }
} } }
} } } ==============================Original
} } Headers==============================
} } } 37, 23 -- From frank_karl-at-lincolnelectric.com
} } Wed Jun 17 09:34:31 2009
} } } 37, 23 -- Received: from lincolnelectric.com
} } (smtp2.lincolnelectric.com [64.109.211.115])
} } } 37, 23 -- Ý Ý Ý by ns.microscopy.com
} } (8.12.11.20060308/8.12.8) with ESMTP id n5HEYUUt015681
} } } 37, 23 -- Ý Ý Ý for {microscopy-at-microscopy.com} ;
} } Wed, 17 Jun 2009 09:34:31 -0500
} } } 37, 23 -- In-Reply-To: {200906171216.n5HCGEvf028336-at-ns.microscopy.com}
} } } 37, 23 -- Subject: Lab envy?
} } } 37, 23 -- Sensitivity:
} } } 37, 23 -- To: Microscopy-at-microscopy.com
} } } 37, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November
} } 30, 2005
} } } 37, 23 -- Message-ID:
} } {OFD94177D7.32F79557-ON852575D8.004434AB-852575D8.00500726-at-lincolnelectric.com}
} } } 37, 23 -- Date: Wed, 17 Jun 2009 10:34:13 -0400
} } } 37, 23 -- From: Frank_Karl-at-lincolnelectric.com
} } } 37, 23 -- X-MIMETrack: CD-MIME by Router on
} } Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } } 37, 23 -- Ý07, 2008) at 06/17/2009 10:34:13 AM,
} } } 37, 23 -- Ý Ý Ý CD-MIME complete at 06/17/2009
} } 10:34:13 AM,
} } } 37, 23 -- Ý Ý Ý Itemize by Router on
} } Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } } 37, 23 -- Ý07, 2008) at 06/17/2009 10:34:13 AM,
} } } 37, 23 -- Ý Ý Ý Serialize by Router on
} } Notescom1/Lincoln Electric/US(Release 8.0.1|February
} } } 37, 23 -- Ý07, 2008) at 06/17/2009 10:34:13 AM,
} } } 37, 23 -- Ý Ý Ý Serialize complete at 06/17/2009
} } 10:34:13 AM
} } } 37, 23 -- MIME-Version: 1.0
} } } 37, 23 -- Content-Type: text/plain;
} } } 37, 23 -- Ý Ý Ý charset="US-ASCII"
} } } ==============================End of -
} } Headers==============================
} } }
} }
} }
} }
} } --
} } "America believes in education; the average professor earns
} } more money
} } in a year than a professional athlete earns in a whole
} } week." Evan
} } Esar
} }
} }
} } ==============================Original
} } Headers==============================
} } 16, 35 -- From kraftpiano-at-gmail.com
} } Thu Jun 18 09:27:13 2009
} } 16, 35 -- Received: from mail-fx0-f212.google.com
} } (mail-fx0-f212.google.com [209.85.220.212])
} } 16, 35 -- ÝÝÝ by ns.microscopy.com
} } (8.12.11.20060308/8.12.8) with ESMTP id n5IERCKB010938
} } 16, 35 -- ÝÝÝ for {microscopy-at-microscopy.com} ;
} } Thu, 18 Jun 2009 09:27:13 -0500
} } 16, 35 -- Received: by fxm8 with SMTP id 8so1216136fxm.18
} } 16, 35 --Ý Ý Ý ÝÝÝfor {microscopy-at-microscopy.com} ;
} } Thu, 18 Jun 2009 07:27:12 -0700 (PDT)
} } 16, 35 -- DKIM-Signature: v=1; a=rsa-sha256;
} } c=relaxed/relaxed;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝd=gmail.com; s=gamma;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝh=domainkey-signature:mime-version:received:in-reply-to:references
} } 16, 35 --Ý Ý Ý Ý Ý
} } :date:message-id:subject:from:to:content-type
} } 16, 35 --Ý Ý Ý Ý Ý
} } :content-transfer-encoding;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝbh=yfEOxXZjFaJVZWpimJ3DTyy4QkaXxp7E8zTwuSF4tyc=;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝb=pjAQ84eqhC8j17eNYNpQuwA4ZWrLRrS6lMo61RHFK38DOOCuj92zExY/ZDf6cxRwjl
} } 16, 35 --Ý Ý Ý Ý Ý
} } J5hdOMA6/ShdUV4efOJkY9lZ+b9ck9EYhoO+6WT7x9SPPnkeSoJ6LhioOsg9KCpgmf5z
} } 16, 35 --Ý Ý Ý Ý Ý
} } 2X2G6vNYfCs8zqoBVf8A1Hd48KWAtKGtbaw1M=
} } 16, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝd=gmail.com; s=gamma;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝh=mime-version:in-reply-to:references:date:message-id:subject:from:to
} } 16, 35 --Ý Ý Ý Ý Ý
} } :content-type:content-transfer-encoding;
} } 16, 35 --Ý Ý Ý
} } ÝÝÝb=SgXkMFlx7JRuB68JWqlN1ylqW97RV5Aw8gNQLuhZ+pBnO+PmFkz7QEmXIrSWb9wsL3
} } 16, 35 --Ý Ý Ý Ý Ý
} } O+X3gE+d3Rsrdr61KHHjBUwQ1qzwgR6Cn7vK2xGZfkkuEDoLN4bsNiayp0/jN6Ag6Vvt
} } 16, 35 --Ý Ý Ý Ý Ý
} } Jpp5eIGZ7a2P4FRqSPjctTdc8NOgcZB8s5vvk=
} } 16, 35 -- MIME-Version: 1.0
} } 16, 35 -- Received: by 10.204.101.2 with SMTP id
} } a2mr1403069bko.104.1245335231608; Thu,
} } 16, 35 -- ÝÝÝ 18 Jun 2009 07:27:11 -0700
} } (PDT)
} } 16, 35 -- In-Reply-To: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
} } 16, 35 -- References: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
} } 16, 35 -- Date: Thu, 18 Jun 2009 10:27:11 -0400
} } 16, 35 -- Message-ID:
} } {25e2b0d20906180727v4e117e2fmc9ad3907075eb4f6-at-mail.gmail.com}
} } 16, 35 -- Subject: Re: [Microscopy] Lab envy?
} } 16, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
} } 16, 35 -- To: Frank_Karl-at-lincolnelectric.com,
} } microscopy-at-microscopy.com
} } 16, 35 -- Content-Type: text/plain; charset=ISO-8859-1
} } 16, 35 -- Content-Transfer-Encoding: 8bit
} } 16, 35 -- X-MIME-Autoconverted: from quoted-printable to
} } 8bit by ns.microscopy.com id n5IERCKB010938
} } ==============================End of -
} } Headers==============================
} }
}
}
}
}
}
} ==============================Original Headers==============================
} 12, 22 -- From vapatpxs-at-yahoo.com Thu Jun 18 11:55:35 2009
} 12, 22 -- Received: from
} web46114.mail.sp1.yahoo.com
} (web46114.mail.sp1.yahoo.com [68.180.199.131])
} 12, 22 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with SMTP id
} n5IGtZTO013213
} 12, 22 -- for {Microscopy-at-Microscopy.Com} ;
} Thu, 18 Jun 2009 11:55:35 -0500
} 12, 22 -- Received: (qmail 95991 invoked by uid
} 60001); 18 Jun 2009 16:55:33 -0000
} 12, 22 -- DKIM-Signature: v=1; a=rsa-sha256;
} c=relaxed/relaxed; d=yahoo.com; s=s1024;
} t=1245344132;
} bh=fP+FEljZC8gqyX+LReJdb9V2qNJLO+S1c6/yskIo8/0=;
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
} b=tvS2IrrG+XW7z9YDmMfGPp360FpHFsT8vhS3nm2QPmqqOs5RG9AXysQ84qkoT5JUPGBEBbwMJL2lzFFH6YvuxRP/qFuOAiYR7Ol7p5YeOfVKLs/P+6ocspS869sG1yxPxL7CNG6//YG+z3TgoIYzoOCAHc9frShLUOlmxB+E9Cw=
} 12, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 12, 22 -- s=s1024; d=yahoo.com;
} 12, 22 --
} h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
} 12, 22 --
} b=bIDaHugfDxHI0Dx+HFiFkBop2SZDiw9QZg/hOBNRh1xqT1KZHkrFfImd6ALgSSOZYTjKFydtEJO6Ov2INMP6jJPYmjFeU3LsmmMBbrLTcrgf5KcaTu//NYIls/8GhnZ3h/CIX2xbKcx9cUuxVayaB9Y5dg6/TnjCo4GPLqJeGBI=;
} 12, 22 -- Message-ID: {518693.95135.qm-at-web46114.mail.sp1.yahoo.com}
} 12, 22 -- X-YMail-OSG:
} jEq7qqAVM1m3X9z8yc0.MViPOx7qjRweq4ti1vdIxy2xOeYBToETPeOGPSZvSySPhKUrdJ65Y9gtPwIDCpw9gkrXxvCq3wiiMwm6lhz5ez.9eNeZ1isOvIn.vSPeWP.L6x1m5knvOJb8Rmteba6cSfXyT5MoT8UQJJNmvP3NMYin0AaSaCjkSYYSF3XptgH3H0EEhsIj7_Zy5qR2vAdurBD8KDLdMqsz3zhMSge3m3.uvomrEdcpi5YarP15LsWV7hyTZfFlK.Fk0uKU_yh4VGePG7X1DzrYgZOYIz6.sFOLrrKLwDgrpduC9U7VirrQTGUBvoeiXGHnXE5v1OzpSAW2N9qjSOuqmtjN4IqczZM-
} 12, 22 -- Received: from [132.239.85.200] by
} web46114.mail.sp1.yahoo.com via HTTP; Thu, 18
} Jun 2009 09:55:32 PDT
} 12, 22 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
} 12, 22 -- Date: Thu, 18 Jun 2009 09:55:32 -0700 (PDT)
} 12, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
} 12, 22 -- Subject: Re: [Microscopy] Re: Lab envy?
} 12, 22 -- To: kraftpiano-at-gmail.com, MSA BB {Microscopy-at-Microscopy.Com}
} 12, 22 -- MIME-Version: 1.0
} 12, 22 -- Content-Type: text/plain; charset=iso-8859-1
} 12, 22 -- Content-Transfer-Encoding: 8bit
} 12, 22 -- X-MIME-Autoconverted: from
} quoted-printable to 8bit by ns.microscopy.com id
} n5IGtZTO013213
} ==============================End of - Headers==============================

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
6, 27 -- From oshel1pe-at-cmich.edu Thu Jun 18 13:19:32 2009
6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IIJVTY029737
6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 13:19:31 -0500
6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n5IIJNvG008018
6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 14:19:30 -0400
6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Thu, 18 Jun 2009 14:19:29 -0400
6, 27 -- Mime-Version: 1.0
6, 27 -- Message-Id: {f06240808c66035414cf3-at-[141.209.160.249]}
6, 27 -- In-Reply-To: {200906181659.n5IGxOlH018977-at-ns.microscopy.com}
6, 27 -- References: {200906181659.n5IGxOlH018977-at-ns.microscopy.com}
6, 27 -- Date: Thu, 18 Jun 2009 14:19:26 -0400
6, 27 -- To: Microscopy-at-microscopy.com
6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 27 -- Subject: Re: [Microscopy] Lab envy?
6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
6, 27 -- X-OriginalArrivalTime: 18 Jun 2009 18:19:29.0305 (UTC) FILETIME=[51D25890:01C9F041]
6, 27 -- X-Canit-CHI2: 0.00
6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 27 -- X-Spam-Score: -1.60 () [Tag at 5.00] L_EXCH_MF,L_USD,MIME_QP_LONG_LINE,RDNS_NONE,SARE_MILLIONSOF,_L_SUPPORT,Bayes(0.0001,-0.5)
6, 27 -- X-CanItPRO-Stream: default
6, 27 -- X-Canit-Stats-ID: 14559400 - ef830b4669fb
6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
6, 27 -- Content-Transfer-Encoding: 8bit
6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IIJVTY029737
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Thu, 18 Jun 2009 13:25:48 -0500
Subject: [Microscopy] SEM Prep mosquito eggs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

I have a researcher here who is studying mosquito eggs. They are 100x300 µm and have an extremely delicate membrane surrounding them. I seem to only get good preservation via CPD out of amyl acetate. Problem is getting enough samples in the chamber to be useful. I received 30 last time and it took a week and a half to prepare them all as I can only get 3 samples in the CPD chamber using the filter membrane holders- and it uses A LOT of expensive solvent.

I just found out I have about 300 more samples coming and can expect this numerous times over the next year or so. The look I got when I glanced at my volunteer as she slunk away was priceless. Have any of you practical experience with preparing samples this small yet in quantity? I have a few ideas and will test them out but no sense in reinventing the wheel.

One thought I had was using glass pipette tips, suck them in and plug with something permeable(agarose maybe or would it shrink too much and fall out).

Another is little bags of dialysis tubing with them floating around inside. Is amyl acetate too big to exchange?

Yet another is some very small glass vials with TEM grids glued on the end. However glued on is difficult to open. Anything less permanent yet solvent resistant?

With either of these I could do at least 30 a day but wanted to poll the list for less theoretical contraptions.



Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891



==============================Original Headers==============================
12, 26 -- From WHITTAKS-at-si.edu Thu Jun 18 13:25:47 2009
12, 26 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IIPlmu006936
12, 26 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 13:25:47 -0500
12, 26 -- Received: from SI-MSESMTPO-02.US.SINET.SI.EDU (SI-MSESMTP-N2.us.sinet.si.edu [160.111.49.76])
12, 26 -- by si-mailout04.si.edu (Postfix) with ESMTP id 4817EFB62
12, 26 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 14:21:55 -0400 (EDT)
12, 26 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by SI-MSESMTPO-02.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959);
12, 26 -- Thu, 18 Jun 2009 14:25:46 -0400
12, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 26 -- Content-class: urn:content-classes:message
12, 26 -- MIME-Version: 1.0
12, 26 -- Content-Type: text/plain;
12, 26 -- charset="iso-8859-1"
12, 26 -- Subject: SEM Prep mosquito eggs
12, 26 -- Date: Thu, 18 Jun 2009 14:25:46 -0400
12, 26 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA303E50B2C-at-SI-ECL02.US.SINET.SI.EDU}
12, 26 -- X-MS-Has-Attach:
12, 26 -- X-MS-TNEF-Correlator:
12, 26 -- Thread-Topic: SEM Prep mosquito eggs
12, 26 -- Thread-Index: AcnwQjEQ6TR4jsy8SbGmSDxtCzB5sw==
12, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
12, 26 -- To: {microscopy-at-microscopy.com}
12, 26 -- X-OriginalArrivalTime: 18 Jun 2009 18:25:46.0882 (UTC) FILETIME=[32E00620:01C9F042]
12, 26 -- Content-Transfer-Encoding: 8bit
12, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IIPlmu006936
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 18 Jun 2009 15:00:38 -0500
Subject: [Microscopy] Re: Lab envy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Justin, you gave me a good laugh!

What about an episode involving filament blow-up of a JEOL TEM and the waiting for the filament to come from Japan?

Stephane



----- Original Message ----
X-from: "kraftpiano-at-gmail.com" {kraftpiano-at-gmail.com}
To: nizets2-at-yahoo.com
Sent: Thursday, June 18, 2009 4:32:03 PM

Perhaps we have discovered a new revenue center for research labs- film sets!

I offer (With a wink and apologies) these suggestions for the Fall
2009 prime time lineup:

Johns Hopkins 90210:
A group of young, talented lab techs fall in and out of love over
the years as they wait for bacterial cultures to multiply in their
pursuit of an unknown virus which has been linked with coffee
addiction.

FIB: Miami
Horatio grows impatient as Eric and Ryan troubleshoot vacuum
issues before being able to modify a prototype semiconductor to test
compatibility with a new set of specifications.

NIST:
Gibbs and Denozzo follow leads in the relentless pursuit of
uncalibrated equipment, as Abby finally reveals that she had
contracted out to four other labs, and that is how she was able to get
everything done.

Lab:
Dr. Gregory Lab sees patients who present him with a myriad of
difficult symptoms, prescribes an antibiotic, and the patient gets
better. Lab tries to follow up by obtaining an actual diagnosis, but
ends up getting bogged down with more patients, escapes to the golf
course instead.



My apologies if I have offended anyone, that was not my intention. I
have deepest respect for those of you on the forefront of research. I
just wanted to give you a little chuckle.

--Justin A. Kraft


On Wed, Jun 17, 2009 at 10:39 AM, {Frank_Karl-at-lincolnelectric.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I can't disagree with any of it. But then,
}
} The public believes:
} That ordinary police discharge their handguns at criminals several times a
} year,
} a person recovers from a gun shot in a less than TV month
} district attorneys try and win 99% of every arrest
} brilliant doctors constantly save every patient while angering patients and
} support staff
} Spies/agents are always good-looking, dashing and resourceful and leave a
} undetected trail of bodies
} WWII prisoners of war constantly got the upper hand on their captors and
} ran resistance cells from the camp
} Bosses are stupid and out witted by carefree employees..
}
} Must I go on....
}
} Entertainment almost always departs from reality and presents a distorted
} view. If it didn't, it would be a documentary and not what most of us
} would call entertainment.
}
} Can we do anything about it? Yes, starting with answering questions from
} friends who say..."Did you see... Can they really do that?"
} Offer to lecture at high Schools, Civic organizations and book clubs.
}
} stay safe.........
}
}
}
}
}
}
} frah0010-at-umn.edu
}
} 06/17/2009 08:16 To
} AM frank_karl-at-lincolnelectric.com
} cc
}
} Please respond to Subject
} frah0010-at-umn.edu [Microscopy] Re: Lab envy?
}
}
}
}
}
}
}
}
}
}
}
}
}
} ----------------------------------------------------------------------------
}
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} gary-at-gaugler.com wrote:
}
} } I don't see why any of us should give any time or effort to this
} } useless venue. . .
}
} One reason we should care about the portrayal of microscopy in films
} and TV (other than simply amusing ourselves) is that it influences how
} the public thinks about microscopy, science, research, etc. I suspect
} that more people have seen, say, "Spiderman" than have ever seen or
} used a real electron microscope or even had the use and function of
} one explained to them. Millions of people watch CSI every week, and
} these people are voters, taxpayers, jury members, and consumers of all
} sorts of scientific and medical information, from global warming to
} the H1N1 outbreak. The portrayal of microscopy and science in popular
} culture is how many people get their information, however right or
} wrong.
}
} If you're looking for how sureality can affect reality, consider the
} so-called CSI Effect:
}
} http://www.usatoday.com/news/nation/2004-08-05-csi-effect_x.htm
} http://www.legalzoom.com/legal-articles/the-csi-effect-juries-demand.html
} http://www.thepocketpart.org/2006/02/thomas.html
}
} If our clients or students see microscopy and science portrayed badly
} in films and TV, they can carry those expectations into the real
} world. Many new users of the electron microprobe in my lab expect it
} to operate under what I call the "microwave oven paradigm" -- open the
} door and put in a sample, press one or two settings, hit the "Analyze"
} button, wait a minute or two, and you're done! I haven't done any
} surveys to learn exactly how they form such misconceptions, but I'd
} hardly be surprised if the portrayal of scientific instruments on TV
} had a lot to do with it.
}
} The point of the discussion isn't really to change Hollywood. In
} fact, I'd argue that we shouldn't -- even I don't want to watch five
} minutes of doing a sample change in the middle of a film before the
} scientist identifies the mysterious material as otherworldly.
} Instead, we should be aware of the misconceptions that many people
} have and that we need to expel before teaching them to use the
} instrument or handing them their data. Scientists need to be more
} aware of and involved in the public perception of science.
}
} Best,
} Ellery
}
} -----------------
} Ellery Frahm
} Senior Research Fellow, Department of Geology & Geophysics
} Manager & Principal Analyst, Electron Microprobe Lab
} Doctoral Candidate, Department of Anthropology
} University of Minnesota - Twin Cities campus
} http://umn.edu/~frah0010
}
}
}
}
}
} ==============================Original
} Headers==============================
} 13, 19 -- From frah0010-at-umn.edu Wed Jun 17 07:07:33 2009
} 13, 19 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu
} [134.84.119.32])
} 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n5HC7VNN015839
} 13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
} 07:07:31 -0500
} 13, 19 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net
} [75.72.182.206])
} 13, 19 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
} 13, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009
} 07:07:31 -0500 (CDT)
} 13, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net
} [75.72.182.206] #+TS+AU+HN
} 13, 19 -- Message-Id: {F14FB1FC-EF3B-4452-9F4C-1B76AECAA9C9-at-umn.edu}
} 13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu}
} 13, 19 -- To: microscopy-at-microscopy.com
} 13, 19 -- In-Reply-To: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes
} 13, 19 -- Content-Transfer-Encoding: 7bit
} 13, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
} 13, 19 -- Subject: Re: [Microscopy] Lab envy?
} 13, 19 -- Date: Wed, 17 Jun 2009 07:07:30 -0500
} 13, 19 -- References: {200906170456.n5H4uImR029033-at-ns.microscopy.com}
} 13, 19 -- X-Mailer: Apple Mail (2.935.3)
} ==============================End of -
} Headers==============================
}
}
} --
} *************************************************************
} Note:
} The information contained in this message may be
} privileged and confidential and protected from disclosure. If
} the reader of this message is not the intended recipient, or
} an employee or agent responsible for delivering this message
} to the intended recipient, you are hereby notified that any
} dissemination, distribution or copying of this communication
} is strictly prohibited. If you have received this
} communication in error, please notify us immediately by
} replying to the message and deleting it from your computer.
} Thank you,
} The Lincoln Electric Company
} **************************************************************
}
}
} ==============================Original Headers==============================
} 37, 23 -- From frank_karl-at-lincolnelectric.com Wed Jun 17 09:34:31 2009
} 37, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
} 37, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5HEYUUt015681
} 37, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jun 2009 09:34:31 -0500
} 37, 23 -- In-Reply-To: {200906171216.n5HCGEvf028336-at-ns.microscopy.com}
} 37, 23 -- Subject: Lab envy?
} 37, 23 -- Sensitivity:
} 37, 23 -- To: Microscopy-at-microscopy.com
} 37, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
} 37, 23 -- Message-ID: {OFD94177D7.32F79557-ON852575D8.004434AB-852575D8.00500726-at-lincolnelectric.com}
} 37, 23 -- Date: Wed, 17 Jun 2009 10:34:13 -0400
} 37, 23 -- From: Frank_Karl-at-lincolnelectric.com
} 37, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 -- CD-MIME complete at 06/17/2009 10:34:13 AM,
} 37, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
} 37, 23 -- 07, 2008) at 06/17/2009 10:34:13 AM,
} 37, 23 -- Serialize complete at 06/17/2009 10:34:13 AM
} 37, 23 -- MIME-Version: 1.0
} 37, 23 -- Content-Type: text/plain;
} 37, 23 -- charset="US-ASCII"
} ==============================End of - Headers==============================
}



--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar


==============================Original Headers==============================
16, 35 -- From kraftpiano-at-gmail.com Thu Jun 18 09:27:13 2009
16, 35 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212])
16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IERCKB010938
16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 09:27:13 -0500
16, 35 -- Received: by fxm8 with SMTP id 8so1216136fxm.18
16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 07:27:12 -0700 (PDT)
16, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 35 -- d=gmail.com; s=gamma;
16, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
16, 35 -- :date:message-id:subject:from:to:content-type
16, 35 -- :content-transfer-encoding;
16, 35 -- bh=yfEOxXZjFaJVZWpimJ3DTyy4QkaXxp7E8zTwuSF4tyc=;
16, 35 -- b=pjAQ84eqhC8j17eNYNpQuwA4ZWrLRrS6lMo61RHFK38DOOCuj92zExY/ZDf6cxRwjl
16, 35 -- J5hdOMA6/ShdUV4efOJkY9lZ+b9ck9EYhoO+6WT7x9SPPnkeSoJ6LhioOsg9KCpgmf5z
16, 35 -- 2X2G6vNYfCs8zqoBVf8A1Hd48KWAtKGtbaw1M=
16, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
16, 35 -- d=gmail.com; s=gamma;
16, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
16, 35 -- :content-type:content-transfer-encoding;
16, 35 -- b=SgXkMFlx7JRuB68JWqlN1ylqW97RV5Aw8gNQLuhZ+pBnO+PmFkz7QEmXIrSWb9wsL3
16, 35 -- O+X3gE+d3Rsrdr61KHHjBUwQ1qzwgR6Cn7vK2xGZfkkuEDoLN4bsNiayp0/jN6Ag6Vvt
16, 35 -- Jpp5eIGZ7a2P4FRqSPjctTdc8NOgcZB8s5vvk=
16, 35 -- MIME-Version: 1.0
16, 35 -- Received: by 10.204.101.2 with SMTP id a2mr1403069bko.104.1245335231608; Thu,
16, 35 -- 18 Jun 2009 07:27:11 -0700 (PDT)
16, 35 -- In-Reply-To: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
16, 35 -- References: {200906171439.n5HEdGAL024315-at-ns.microscopy.com}
16, 35 -- Date: Thu, 18 Jun 2009 10:27:11 -0400
16, 35 -- Message-ID: {25e2b0d20906180727v4e117e2fmc9ad3907075eb4f6-at-mail.gmail.com}
16, 35 -- Subject: Re: [Microscopy] Lab envy?
16, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
16, 35 -- To: Frank_Karl-at-lincolnelectric.com, microscopy-at-microscopy.com
16, 35 -- Content-Type: text/plain; charset=ISO-8859-1
16, 35 -- Content-Transfer-Encoding: 8bit
16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IERCKB010938
==============================End of - Headers==============================





==============================Original Headers==============================
31, 22 -- From nizets2-at-yahoo.com Thu Jun 18 15:00:38 2009
31, 22 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
31, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5IK0be3029855
31, 22 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 15:00:38 -0500
31, 22 -- Received: (qmail 34183 invoked by uid 60001); 18 Jun 2009 20:00:37 -0000
31, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245355237; bh=5JlVVAK5cFXJP8EstJIVjen4AV7BioSvKsviacl8cX4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type; b=WpXaBVy9P3gE3SHTbANWuqTwtvYiGawW+11ZuTqKU4vEl/vqW/JEYjT7J2VLxrw5TlSHT8pIf3dYA4/KDxmZxdT7Ljl0Xf9EF2FZaB4cPvTQr82HDnslUJmZUA+qGySsfQMdntfjMkOIfvCqHth4ORAdINMIppR9vUD/jCKGuls=
31, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
31, 22 -- s=s1024; d=yahoo.com;
31, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type;
31, 22 -- b=h3uMhQ/GVW/EKFY4VKGz17Eb22qwRQaWoEQnm6FKTOiHFthSHC50328JXRWUe4XpBYwn1pTiXtZzttPTKmcH2Hm9V3v7viYq5FtElOd9a/JwFzl2KUjlXz+j3YwrjKvcqsAqA+a4pNHFdxXl4iNR4r3Qk+eoBItyFP+45GfoLoc=;
31, 22 -- Message-ID: {145960.32750.qm-at-web110804.mail.gq1.yahoo.com}
31, 22 -- X-YMail-OSG: GkgLlGUVM1nxBeCpDfWZjWUu2tbH3hbbZmeK9wIs6NTxb5DpMGFqiIeRotdksDhodPC3vJJMf.7ExwPrqzffrzn2ZRuoTQh_Hj5QFdfLh3m0YyLwm6kskiJBzpCJjKg5MVOK9S3_S0x_iWxw_gPBn9fkzbf71BEJEM2r.mmgGykYKNAbKXglnwUH1JDpkcR_b2b4PWjKA_CbheOYnmepaDXLeyyWQynwYGjUqd3Zpj89naER7Q_L5zBujxe1w1OHJtgLzba1JXrXN.psFEH.dwk4Rz_Uhyc3Na4mdbJk7KEYUGjjg3ZbXR4bRFuIcBQ4SbRwc9Qk.onzEVxu3aAVgBhlZsmbcmXv1Iid6VM-
31, 22 -- Received: from [80.121.4.68] by web110804.mail.gq1.yahoo.com via HTTP; Thu, 18 Jun 2009 13:00:36 PDT
31, 22 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
31, 22 -- References: {200906181432.n5IEW3Jj017370-at-ns.microscopy.com}
31, 22 -- Date: Thu, 18 Jun 2009 13:00:36 -0700 (PDT)
31, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
31, 22 -- Subject: Re: [Microscopy] Re: Lab envy?
31, 22 -- To: microscopy-at-microscopy.com
31, 22 -- In-Reply-To: {200906181432.n5IEW3Jj017370-at-ns.microscopy.com}
31, 22 -- MIME-Version: 1.0
31, 22 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 18 Jun 2009 15:11:50 -0500
Subject: [Microscopy] Re: :LM vs TEM immunostain patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is absolutely no doubt that localization and function are closely related.
One of the best example I like to give is the well-known NF-kB pathway. The transcription factor NF-kB is inhibited by interacting with the so-called ikB (inhibitor of NF-kB) and its subsequent sequestration in the cytoplasm (you can imagine how hard it is to activate the transcription in the cell cytoplasm)! In this simple example the location of NF-kB is a critical parameter to determine if the transcription factor is active or not.

Stéphane




----- Original Message ----
X-from: "underwoo-at-u.washington.edu" {underwoo-at-u.washington.edu}
To: nizets2-at-yahoo.com
Sent: Thursday, June 18, 2009 5:40:57 PM

Hello All,
I appreciate this discussion and agree with both of you. There is a balance to be struck between understanding these types of phenomenon and the pragmatic side of getting the work completed with the best bang for the buck. As usual, in my position, I am caught in the middle. Each time I am confronted with such a phenomenon I am compelled to understand it in order to be confident of the result but mostly to improve the results and interpretation of future experiments. I admit, however, that if I had informed the people who pay my salary how much time and resources has been spent to make each incremental improvement, I would have been fired long ago. I still believe that what they don’t know helps them in the long run and passionately believe that the hypothetical function of a gene product should be supported by morphologic location and the leap to ultrastructural localization is the key to
assigning that function. So having said that, I lie awake at night with fears that a simple component of a cryoprotectant that is so commonly used such as polyvinylpyrrolidone could cause a change in immunolabeling pattern for some proteins. Years of irreplaceable tissue samples are bathed in PVP/sucrose and stored in liquid nitrogen. Just writing that sends -200ºC chills through me. I will continue to pursue it for now and let you know if I find out something relevant.
Thanks for all your suggestions.

Robert Underwood
University of Washington
Dermatology
X-from: nizets2-at-yahoo.com
To: underwoo-at-u.washington.edu


Hi Jan!

When you get a nuclear signal in LM and then your recognition pattern is a
membrane one in EM, you must admit that something went wrong! If the target is
known to be present in the nucleus, you have 99,99% chances that something went
wrong with EM. But of course there remains a 0,01% chances that you just
discovered something worth a paper in Science :-)
This is what I meant when I was talking about "to make sense". I know that we
have to be careful about not being too much influenced by our expectations when
analysing our results, but still we have to stay realistic with what we may
expect. This is a delicate balance indeed and lots of controls always help.

TEM is definitely no confirmative technique, it is a complementary technique to
LM.
Actually TEM is most often used not only to confirm but to increase the
precision of a labeling pattern in LM and much less often to deny molecular
biology data. In LM you can "see a spot" in the nucleus. In EM, you can say
which nuclear structure is involved and even which part of this nuclear
structure. It is not a matter of just confirming the LM pattern, it is a very
important improvement upon LM results.

Best regards from the sunshiny Vienna, at the roots of the alps and the border
of the not-so-blue Danube
And, incidentally, the best city in world to live in!
(see http://www.citymayors.com/features/quality_survey.html)

Stephane




----- Original Message ----
X-from: "leunissen-at-aurion.nl" {leunissen-at-aurion.nl}
To: nizets2-at-yahoo.com
Sent: Thursday, June 18, 2009 7:09:14 AM

Dear Stéphane,

This is a very interesting discussion and I hope it will stimulate
more responses from other members as we go "more fundamental". We are
perhaps moving away a little from the original thread. However, the
topic you addressed in the first line of your message challenges me to
give further explanations.

Even though the difference between limited penetration and limited
accessibility is fundamental, the end result may be the same and I
assume that is what you refer to. Both result in low signals,
sometimes too low to be convincing. To increase labeling efficiency it
is important to understand the mechanisms. Specimen characteristics
and specimen preparation control the environment of the antigen. When
antigens are encapsulated and therefore inaccessible, than that may be
something one has to accept for instance with antigens in acrylate
sections. On the other hand sometimes procedures can be applied that
expose masked antigens, e.g. by etching. Limited penetration on the
other hand is related to tissue characteristics as well as to the
reagents, and the development of smaller secondary gold conjugates
such as Auroprobe One, Undecagold, Nanogold and UltraSmall Gold
conjugates aimed at increasing labeling efficiency, and successfully so.

The pragmatic approach is a personal choice, certainly, and it is a
compromise. There may be many reasons why one would choose for a
pragmatic approach, one being the pressure on research funding. I
experience that in projects I am involved with too and it does not
always make me happy to be frank. As is the case with any compromise,
the chance for a pragmatic approach to be successful depends on how
well-informed one is about the options and this is where I hope to
contribute. Personally, whenever possible I choose the way that
explores more in-depth, in product development for instance. Going in-
depth in one area is probably what allows being pragmatic in another
area.

When you say "In the end, what matters here is not to understand the
precise mechanisms of labeling but to get a recognition patter of a
transcription factor which makes sense in the given context" I have to
disagree, especially with the second part. This tends to degenerate EM
to a confirmative technique rather than the unique research technique
status it deserves when employed properly. If we only look for what we
or the PI want to see because it fits nicely in existing concepts,
then we are bound to make only very limited progress, if at all and
why would we do this in the first place? From my personal experience I
know many core facilities find themselves confronted with this issue
and they probably often struggle to get the message across. To me this
seems a very fundamental issue.
When I was still associated with Utrecht university, longer ago than I
like to remember, we did some localization work in a collaboration
project with the microbiology dept. This was in the early days of
genetic engineering, involving multi-copy gene encoded hybrid proteins
which were hoped to be excreted or at least to be built into the outer
membrane of E coli. Biochemical studies using cell fractionation
indicated the hybrid proteins were in the membrane fraction.
Microbiologists happy .... and could we please make them a few
pictures, because it would look nice in the publication! The comic
book approach. Our localization work with immunogold revealed that the
hybrid protein was not IN the membrane but stacked below it on the
cytoplasmic side, blocking export sites etc. It took us a few meetings
to convey the message, you can probably well imagine. I think this
illustrates nicely how a confirmative approach would have made us give
up after a while or would have our immuno EM results to be discarded,
because the microbiologists thought they did not fit and did not like
it. Likewise, people coming from a light microscopy immuno background
often expect EM to give them the same results they obtained in the
light microscope. Without knowing how those results were obtained and
what they are worth, that can be hard! And here we are ... back to the
original topic.

One thing I admit: pragmaticians make for shorter messages!

Best regards everyone, we're heading towards the midwinter solstice
this weekend in New Zealand with a lantern parade and fireworks and
then the days will start to lengthen again!! Yay!
Enjoy the longest day and the magical fires on mountain ridges in some
areas of the Alps in the Northern hemisphere.


Jan


On 17/06/2009, at 8:52 PM, Stephane Nizet wrote:

}
} Hello Jan!
}
} You say that gold conjugates have limited penetration and I say that
} antigens are less accessible. What is the difference?
} I agree that "increasing the antibody concentration" was a bit
} hasty. Actually, having a general weaker signal (because of limited
} penetration), or to say otherwise having less antigens to recognize,
} you may wish to increase the signal in order to see something. I
} know that in this regard I have something to learn from you so I
} will always appreciate your comments on this matter but it all seems
} to make sense to me.
}
} Now on the necessity to understand what happens: it is all a
} question of personal goals and priorities I think.
} But I must say that it is my personal experience that it is better
} to have some limits to explorations. It happened in my career that I
} spent a lot of time to understand a phenomenon and in the end it did
} not bring much because that was not what I was looking for. Now I
} have become more pragmatic: I have a goal and I try not to let me
} disturb with whatever comes during the study. As researchers we are
} all curious in nature, but I think that we have to try and stay
} focused on our goals. In the end, what matters here is not to
} understand the precise mechanisms of labeling but to get a
} recognition patter of a transcription factor which makes sense in
} the given context.
} In this case, if Robert can show a secondary "unspecific" band for a
} component of desmosomes in western blot, the staining of desmosomes
} in EM is OK, because it cannot interfere with a nuclear pattern
} (provided he has a specific nuclear pattern too). The absence of
} desmosome staining in LM does not need to be explained, it may be
} that upon thawing the antigens lost the conformation which allowed a
} correct immunodetection. Well there are possible explanations for
} the absence of staining in LM, they may remain as hypotheses because
} they do not interfere with the primary goal of location the
} transcription factor.
}
} Finally, you wrote "However, using cells that lack certain
} structures and thus do not show labeling of those structures....
} hmmm...not sure about the relevance of that one!". It IS relevant,
} as long as the-said features are not the object of study. If an
} antibody shows background staining in some cells and not in others,
} why not use the cells with low background staining (provided it
} still makes sense of course)? In the case I provided, I was talking
} about keratinocytes and skin fibroblasts because Robert is working
} with skin. Not because of the absence of desmosomes, but the absence
} of desmosomes is simply an addtional comfort.
}
} Best regards,
} Stéphane
}
}
}
}
} ----- Original Message ----
} From: Jan Leunissen {leunissen-at-aurion.nl}
} To: nizets2-at-yahoo.com
} Sent: Wednesday, June 17, 2009 1:23:31 AM
} Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
}
} Dear Stephane,
}
} Thank you for your appreciation. I do appreciate your slight
} disagreement and would like to add that hopefully from difference of
} opinions there will be in the end a useful contribution to
} explaining the phenomenon that Robert describes.
}
} I think you will agree that researchers have a responsibility to try
} to explain differences that may arise from different approaches. I
} would think it mandatory where the outcome of those approaches
} (being LM and EM immunodetection) are different, but where
} nevertheless an end result is put in the same wording "this or that
} antigen localizes there and there". In Robert's case he has made a
} big effort to keep many of the parameters involved very similar or
} even exactly the same. And exactly that makes the difference that
} much more difficult to understand and thus so interesting and
} challenging. In my opinion the appropriate way forward would be to
} further eliminate the number of remaining procedural parameters in
} an attempt to increase the reliability of the outcome and to
} understand what's going on.
}
} Allow me to comment to a few of the issues you raise.
}
} I did not say antigens are usually less accessible in TEM. I stated
} that 5 and 10 nm gold conjugates do not penetrate readily, even in
} hydrated Tokuyasu sections. The solution is not using higher primary
} concentrations but using a secondary reagent that penetrates more
} easily.
}
} What is the reason for using higher antibody concentrations, if you
} assume antigens are only limitedly accessible, e.g. only at the
} section surface? Those antigens do not "know" they are the only ones
} available for binding. In general, any interaction between those
} antigens and antibodies would only be different if there is a
} difference in avidity binding, or when the antigen was altered to
} affect affinity binding. That does not seem likely in the present
} case, as the material was treated much the same, but ... often there
} are differences without us being aware.
}
} Although maybe not directly relevant for the observations of Robert,
} I do agree that different antibody concentrations may result in
} detecting different antigens/epitopes or molecular structures that
} are very similar in surface structure. Or that the relative signal
} densities may differ. This certainly can be the cause of
} misinterpretations. Antibody concentrations determine what the
} antibodies bind to. This raises the question: which concentration
} (range) is the right one? I wish there was an easy answer, but there
} isn't. In immunochemistry parameters and variables are much more
} easy to establish and understand than in immunohisto- and
} immunocytochemistry. In tissue, after fixation, dehydration,
} freezing, thawing we are likely facing a vast range of modifications
} to the original antigen, both physically and chemically. So being
} consistent, comparing apples with apples, as Leona Cohen-Gould puts
} it, is the way to go.
}
} Strictly, finding a certain antigen pattern in cells does only mean
} that that is the situation for those cells at best, under those
} particular circumstances. Comparing localization in cells with
} tissues should therefore be done with caution, but may certainly
} help. However, using cells that lack certain structures and thus do
} not show labeling of those structures.... hmmm...not sure about the
} relevance of that one!
}
} In my previous message I stated: "...it is very worthwhile to
} understand what goes on. If not, what do we decide is real? The
} label pattern we like, the one that suits us best?" Keeping an open
} mind for what we see, for the results we obtain and making an effort
} to understand those should also discriminate researchers from finders.
}
}
} Cheers,
}
} Jan


==============================Original Headers==============================
13, 20 -- From leunissen-at-aurion.nl Thu Jun 18 00:04:59 2009
13, 20 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5I54w3d024838
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 00:04:59 -0500
13, 20 -- Received: from [192.168.1.50] (really [122.57.245.108]) by
fep02.xtra.co.nz
13, 20 -- with ESMTP
13, 20 -- id {20090618050456.GRJH28374.fep02.xtra.co.nz-at-[192.168.1.50]}
13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 17:04:56
+1200
13, 20 -- Message-Id: {6E10F8DD-CF6A-47F7-AD54-7AB7A4239458-at-aurion.nl}
13, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
13, 20 -- To: microscopy-at-microscopy.com
13, 20 -- In-Reply-To: {609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
13, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
13, 20 -- Subject: Re: [Microscopy] :LM vs TEM immunostain patterns
13, 20 -- Date: Thu, 18 Jun 2009 17:04:55 +1200
13, 20 -- References: {200906160904.n5G94H6N029691-at-ns.microscopy.com}
{AF3B072D-CB00-442E-9E59-930360ABA76E-at-aurion.nl}
{609288.11506.qm-at-web110809.mail.gq1.yahoo.com}
13, 20 -- X-Mailer: Apple Mail (2.935.3)
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n5I54w3d024838
==============================End of - Headers==============================






==============================Original Headers==============================
31, 25 -- From nizets2-at-yahoo.com Thu Jun 18 03:38:33 2009
31, 25 -- Received: from web110805.mail.gq1.yahoo.com
(web110805.mail.gq1.yahoo.com [67.195.13.228])
31, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n5I8cX5b014744
31, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 03:38:33 -0500
31, 25 -- Received: (qmail 33983 invoked by uid 60001); 18 Jun 2009 08:38:32
-0000
31, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1245314312; bh=uGU7yvbfSg3v1O7sTslumbaaKsXX9iBCzLIQWs2ER4Y=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In
-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=4N/GK3AAFOKn2BHjEGEvDpiojTtk4lZUFG3XEVsW0NW6nTUaqwAKEJ+FM7QIgZ/yFi4POK0ckusk2s
pUu2ZPce+lid2CSzV8GGdnVEcVgxQ1ZztRbt3i5wYXZH9Ai/twfCtHwUYC6ruZyaMUNn4PPVOr++nYyN
tNQb99T/uh0cY=
31, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
31, 25 -- s=s1024; d=yahoo.com;
31, 25 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In
-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
31, 25 --
b=0r4hDtFiYNFI4+YbwMQQpBnvRMG0z2xXEfrF1cbwhEe6Y6741XvMwn/JddFIH7EE7cj4ftQD2V4pgx
tPcGVV7eg+/JMF8rJRZXILpBCXQWRAmu1yNPuRk67EJKWtCBmFUOIYt4V5paQEBldUjwDbMVhCOHP32s
P79yYThsgB+AU=;
31, 25 -- Message-ID: {684191.33121.qm-at-web110805.mail.gq1.yahoo.com}
31, 25 -- X-YMail-OSG:
Wkvs334VM1mdN7VjPyDH1hpKd.hSzwz52rOSjNwLssE7H2JNrUyMVb5uaY2.Wu7Napgkx7yqPfOB7ThW
mqPCeXaQGLkNyzBC27XtT4AA9DReSgyleoUd07Tu.mbPufnkp4Jmwg.QqxEkTGhv4IytJPM84_fn2.cc
ilK7sEsdDHyCjBijCMO7CjUmS57RzsjX03Sog0dJTnc7SdECNuJjR_b1BR9wggortgfMhe7u4tPT9i4Z
khjIthgyy.1IDHNQlWvjZqUO4Owp67Erc5ZB5dECCEoIxJwU26yL6sYopqoeKZAivK9JvMYbVqGeC.nD
FHsFyQ2klVDLybigxiRrIlwMB4365rnfVbFSJdHwzoQ-
31, 25 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via
HTTP; Thu, 18 Jun 2009 01:38:32 PDT
31, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
31, 25 -- References: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- Date: Thu, 18 Jun 2009 01:38:32 -0700 (PDT)
31, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
31, 25 -- Subject: Re: [Microscopy] Re: :LM vs TEM immunostain patterns
31, 25 -- To: leunissen-at-aurion.nl
31, 25 -- Cc: microscopy-at-microscopy.com
31, 25 -- In-Reply-To: {200906180509.n5I59Evu031173-at-ns.microscopy.com}
31, 25 -- MIME-Version: 1.0
31, 25 -- Content-Type: text/plain; charset=iso-8859-1
31, 25 -- Content-Transfer-Encoding: 8bit
31, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n5I8cX5b014744
==============================End of - Headers==============================








==============================Original Headers==============================
47, 24 -- From underwoo-at-u.washington.edu Thu Jun 18 10:35:17 2009
47, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134])
47, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5IFZG1V027383
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 10:35:17 -0500
47, 24 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103])
47, 24 -- by mxout1.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.05) with ESMTP id n5IFZFad018188
47, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 08:35:15 -0700
47, 24 -- Received: from localhost (localhost [127.0.0.1])
47, 24 -- by hymn13.u.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) with ESMTP id n5IFZCm4005460
47, 24 -- for {Microscopy-at-microscopy.Com} ; Thu, 18 Jun 2009 08:35:12 -0700
47, 24 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Thu, 18 Jun 2009 08:35:12 PDT
47, 24 -- Date: Thu, 18 Jun 2009 08:35:12 -0700 (PDT)
47, 24 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
47, 24 -- To: Microscopy List {Microscopy-at-microscopy.Com}
47, 24 -- Subject: [Microscopy]: LM vs TEM immuostain pattern changes
47, 24 -- Message-ID: {Pine.LNX.4.43.0906180835120.25964-at-hymn13.u.washington.edu}
47, 24 -- MIME-Version: 1.0
47, 24 -- Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed
47, 24 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.6.18.152115
47, 24 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
47, 24 -- SUPERLONG_LINE 0.05, BODY_SIZE_10000_PLUS 0, ECARD_KNOWN_DOMAINS 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
47, 24 -- Content-Transfer-Encoding: 8bit
47, 24 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n5IFZG1V027383
==============================End of - Headers==============================






==============================Original Headers==============================
63, 25 -- From nizets2-at-yahoo.com Thu Jun 18 15:11:50 2009
63, 25 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
63, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5IKBnDU011759
63, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 15:11:49 -0500
63, 25 -- Received: (qmail 39595 invoked by uid 60001); 18 Jun 2009 20:11:49 -0000
63, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245355909; bh=db+2CYl/xz0I2Jfr16ofXgV5o15v0Rmh6y9HwmmKbu8=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=RiErZ0+24tXbdIuk+q8JFNJAolRy6hv8etPHAHKjlohePmn/F7n7hYVDaxbqZzJMpmaXmS6fimh2NIEAV2DlknZUUg4nTT83T35uABswZIuXdFBcKkszkzQEqivlSkQj49gP4lrGbUAloc4c72CXX3leD1DZ0v+xVZJOcplwteI=
63, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
63, 25 -- s=s1024; d=yahoo.com;
63, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
63, 25 -- b=orfHbcz50YKvBSUtwG+SO+cOJDV17SsYap4NwIAY92d7t//5F8YNoMvLeCcVCHrGfE8t3M14zdCHUNBg+gQ+e8QSFjFOwcHVux1B1MG6zyORao/fLeswiP7OFksy9Zyez+z5ERnwyCRynQpG/T0PFBK1cv4zvcU+Q6xzfkT0FTE=;
63, 25 -- Message-ID: {186585.39420.qm-at-web110804.mail.gq1.yahoo.com}
63, 25 -- X-YMail-OSG: 6nVS2wcVM1muiRTvU72s5RKOVjQiw7p5uAm8HdWcyyh5Vy7EJ7HWyLlDzbF04.V79yHOWD0oEuS7PxK6XpuGke_PNOpe2bp67CdJaArG3ytJobp1mB5KVuq7isq23837ZGKA3oxlzm5vVLh4N07wVjsOCZZ5dmkyTvSyurcelrEwUvsi5LgcieGCJhHdUm3hOtMFA3fCdPfxWm_bF883kINIWUTxvWbmJJLuiEH1CfQn6PoA_tYHx9Xa_mH6AR2UVKr1k2qw3v53hIuGHVh1jIPJ0mP_gwETUTcH0oCdB5ErqkkR0EwpGb4xoCn.uXK6zE.lcq4Gfo6Tf7YVQcAGwMCSql0jEGhso5QnBag-
63, 25 -- Received: from [80.121.4.68] by web110804.mail.gq1.yahoo.com via HTTP; Thu, 18 Jun 2009 13:11:48 PDT
63, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
63, 25 -- References: {200906181540.n5IFevmN001469-at-ns.microscopy.com}
63, 25 -- Date: Thu, 18 Jun 2009 13:11:48 -0700 (PDT)
63, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
63, 25 -- Subject: Re: [Microscopy] : LM vs TEM immuostain pattern changes
63, 25 -- To: underwoo-at-u.washington.edu
63, 25 -- Cc: microscopy-at-microscopy.com
63, 25 -- In-Reply-To: {200906181540.n5IFevmN001469-at-ns.microscopy.com}
63, 25 -- MIME-Version: 1.0
63, 25 -- Content-Type: text/plain; charset=utf-8
63, 25 -- Content-Transfer-Encoding: 8bit
63, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5IKBnDU011759
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Fri, 19 Jun 2009 12:35:09 -0500
Subject: [Microscopy] LR White and immunofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

We have been doing CPD of young mouse embryos. We use a 24 specimen holder
(ours from Electron Microscopy Sciences) . The holes are about 4mm in
diameter arranged in two discs of 12 holes each with a mesh screen on top
and bottom. The outer diameter is about an inch so it fits nicely into our
CPD.

These small tissues are difficult to get out after the CPD so after fixation
we put the holder into water just to the top of the holes, make an envelope
or small cup out of a tiny piece of Kimwipe, place it into the holder hole,
put or pipet the specimen in then close it up snuggly.

To facilitate dehydration (Ethanol) we use two 50 ml Tri-pour beakers fitted
with a shelf made of screen bent into a "U" shape with a stir bar
underneath. The level of ethanol is such that when the specimen holder is
put into the beaker it will be well covered. Put the beaker on a stirrer
for as long as you like. Alternate between the two beakers giving a rinse
with the next solution before filling. I have not had amyl acetate in a
Tri-pour beaker - you'd know if you would need to use glass.

After CPD the tissues come easily out of the holder by grabbing the paper.
The specimen does not get touched. You could "dump" the egg/eggs right onto
the prepped SEM stub without needing to touch them.

Depending on your samples you may be able to do them all in a few runs.

Second thought...
Years ago I worked with rabbit eggs and needed to use 1% Acrolein in my
fixative (glutaraldehyde plus paraformaldehyde) to get a good fix for TEM.
Would that help? If so be advised that acrolein is in tear gas or it acts
like it!
Since the eggs are laid on water, do you use water or buffer for your fix?

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not
represent the NIH. This message is not confidential and can be freely shared
and reproduced.

=======================
X-from: {WHITTAKS-at-si.edu}
Reply-To: {WHITTAKS-at-si.edu}


Happy Friday Everybody,

I have a user who has a precious sample and wants to embed in LR White, maybe. I'm having him practice on something not precious first. My questions are:

1. He wants to do immunofluorescence and morphology on these nerve samples. He is planning on perfusing in 2% PFA, 0.1% glut in 0.1M sodium cacodylate. Then he will dissect out the nerve, cut it in half and place half in 2% PFA, 0.1% glut (embed this in LR White) and the other half in 4% PFA, 2.5% glut (embed this in Epon) to finish the fixation. Will that work? Or will the perfusion fixation with the weaker fix cause the stronger submersion fix to be moot?

2. He wants to do immunofluorescence on the LR white sample. I figure, primary antibody, then secondary with glow is no different that secondary with gold ball, am I correct in this?

Thanks and have a great weekend.

All aglow waiting for your answer,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.




==============================Original Headers==============================
12, 21 -- From vapatpxs-at-yahoo.com Fri Jun 19 12:35:08 2009
12, 21 -- Received: from web46113.mail.sp1.yahoo.com (web46113.mail.sp1.yahoo.com [68.180.199.130])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5JHZ8pZ023043
12, 21 -- for {Microscopy-at-Microscopy.Com} ; Fri, 19 Jun 2009 12:35:08 -0500
12, 21 -- Received: (qmail 55935 invoked by uid 60001); 19 Jun 2009 17:35:07 -0000
12, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245432906; bh=FNx12R/rM5oVszCnFhdGMEOg09f7lhiF25b8JXgn06U=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=H9MzfTpfXtIPvjtYfVF0plTeWszuhB9KejXTsWofMx2dBD6hF280y7v4YiJz4HP0t8SdcchRqmpL5s6W+CiXnwrYk2U5tFYOYXugUG1V+kXWGyEnSv9RMYvlVWkYiWI5wYFeNaCMN1vsTWDrFmNAjQMQr4SEtdd2mI4nmmRWi6g=
12, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 21 -- s=s1024; d=yahoo.com;
12, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 21 -- b=s3KOHX5IKOpLHgSVDqquIgLVoYOh2GRtSClxaH03ot+M3EH0ZPejwYgl+YOZnXVFE/Hv210jsqm3qCJUuXA2nP5xs4jr5A7WeDU1cUP/IT/ZBVHtyNRKPhwAr9udTOHpLWpwpYKdLlNhGqkiVpHnR34kIKBIuw1EuQzIFlAbPRw=;
12, 21 -- Message-ID: {778498.55603.qm-at-web46113.mail.sp1.yahoo.com}
12, 21 -- X-YMail-OSG: xRizKA0VM1mPM1uhlSENLpzIBNwquohwnbHhrHdZ_A.BZ87vZwpX778CpqX8LG.4SunY..oiyGgqhdNl93DtzJMCWLlLv5ovzgJ19ZUnWO58azdhlMUr6bOoYMmNG2C_Pet4EvCYxqeOx0vNFvyBMsmdp2DXJEL2NOQQA7MVu4wS9rXRS568A2VqsV5gsvqjwUrrFxBj5.lZdUoUUf_vflgFNlwZIU6uAiLkpzpqMgDrH0mtgcO7vdZerK6PyZy_ZHweDlCc_0MjV_R.FRgI22mwjPBvA6VqkSuspw--
12, 21 -- Received: from [132.239.85.200] by web46113.mail.sp1.yahoo.com via HTTP; Fri, 19 Jun 2009 10:35:03 PDT
12, 21 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
12, 21 -- Date: Fri, 19 Jun 2009 10:35:03 -0700 (PDT)
12, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 21 -- Subject: LR White and immunofluorescence
12, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
12, 21 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
12, 21 -- MIME-Version: 1.0
12, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Fri, 19 Jun 2009 13:43:02 -0500
Subject: [Microscopy] SEM Prep mosquito eggs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Scott,
Your embryos are about the same size as drosophila embryos which I have
processed many times for SEM in years past. One of the best embryo
containers for CPD is one I made and you can easily make. Purchase some
Nylon or polyethlene (I typically used the Nitex brand of nylon mesh but
other materials work also) mesh of the appropriate size. I usually
worked with 80--90 micrometer opening/mesh size. Cut off the bottom of a
standard Beem embedding capsule so you end up with a cylinder with a
cap. Cut another cap from another Beem capsule that you will use to cap
the the second end of the cut Beem capsule cylinder. Cut a little square
or circle of the Nylon mesh to fit over the end of the Beem capsule with
extra around the edges. Push a cap on which will seal the mesh to the
capsule. I processed the tissue in these open at the top Beem capsules
by placing them, standing up, in a Petri dish filled with fluid and
changing the fluid as needed. After the final ethanol I added the Nylon
mesh and cap to the top of the specimen containing Beem capsule. The
entire capsule or several of them go into the CPD. Fluid transfer is
quite good through the mesh. When the samples are dry I carefully opened
one end of the capsule and tapped the dry embryos onto a sample stub
with one of the various adhesive methods or onto a piece of the very
smooth Ross lens tissue for careful transfer via forceps.
There are various commercial assemblies that can utilize a filter made
with Nylon mesh but I did not find any advantage over Beem capsules.
Best wishes,
Larry


connellyps-at-nhlbi.nih.gov wrote:
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} Scott,
}
} We have been doing CPD of young mouse embryos. We use a 24 specimen holder
} (ours from Electron Microscopy Sciences) . The holes are about 4mm in
} diameter arranged in two discs of 12 holes each with a mesh screen on top
} and bottom. The outer diameter is about an inch so it fits nicely into our
} CPD.
}
} These small tissues are difficult to get out after the CPD so after fixation
} we put the holder into water just to the top of the holes, make an envelope
} or small cup out of a tiny piece of Kimwipe, place it into the holder hole,
} put or pipet the specimen in then close it up snuggly.
}
} To facilitate dehydration (Ethanol) we use two 50 ml Tri-pour beakers fitted
} with a shelf made of screen bent into a "U" shape with a stir bar
} underneath. The level of ethanol is such that when the specimen holder is
} put into the beaker it will be well covered. Put the beaker on a stirrer
} for as long as you like. Alternate between the two beakers giving a rinse
} with the next solution before filling. I have not had amyl acetate in a
} Tri-pour beaker - you'd know if you would need to use glass.
}
} After CPD the tissues come easily out of the holder by grabbing the paper.
} The specimen does not get touched. You could "dump" the egg/eggs right onto
} the prepped SEM stub without needing to touch them.
}
} Depending on your samples you may be able to do them all in a few runs.
}
} Second thought...
} Years ago I worked with rabbit eggs and needed to use 1% Acrolein in my
} fixative (glutaraldehyde plus paraformaldehyde) to get a good fix for TEM.
} Would that help? If so be advised that acrolein is in tear gas or it acts
} like it!
} Since the eggs are laid on water, do you use water or buffer for your fix?
}
} Pat
}
} Patricia Stranen Connelly
} Research Assistant
} NHLBI Electron Microscopy Core
} National Institutes of Health
} 14 Service Road West
} Bldg. 14E ­ Rm. 111B MSC 5570
} Bethesda, MD 20892-5570
} Phone 301-496-3491
} FAX 301-480-6560
} connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
}
} Opinions and experiences related are those of Pat Connelly and do not
} represent the NIH. This message is not confidential and can be freely shared
} and reproduced.
}
} =======================
} X-from: {WHITTAKS-at-si.edu}
} Reply-To: {WHITTAKS-at-si.edu}
} Date: Thu, 18 Jun 2009 14:29:43 -0400
} To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
} Conversation: [Microscopy] SEM Prep mosquito eggs
} Subject: [Microscopy] SEM Prep mosquito eggs
} ----------------------------------------------------------------------------
}
} All,
}
} I have a researcher here who is studying mosquito eggs. They are 100x300 µm
} and have an extremely delicate membrane surrounding them. I seem to only get
} good preservation via CPD out of amyl acetate. Problem is getting enough
} samples in the chamber to be useful. I received 30 last time and it took a
} week and a half to prepare them all as I can only get 3 samples in the CPD
} chamber using the filter membrane holders- and it uses A LOT of expensive
} solvent.
}
} I just found out I have about 300 more samples coming and can expect this
} numerous times over the next year or so. The look I got when I glanced at my
} volunteer as she slunk away was priceless. Have any of you practical
} experience with preparing samples this small yet in quantity? I have a few
} ideas and will test them out but no sense in reinventing the wheel.
}
} One thought I had was using glass pipette tips, suck them in and plug with
} something permeable(agarose maybe or would it shrink too much and fall out).
}
} Another is little bags of dialysis tubing with them floating around inside.
} Is amyl acetate too big to exchange?
}
} Yet another is some very small glass vials with TEM grids glued on the end.
} However glued on is difficult to open. Anything less permanent yet solvent
} resistant?
}
} With either of these I could do at least 30 a day but wanted to poll the
} list for less theoretical contraptions.
}
} Scott Whittaker
} Head NMNH Imaging
} Manager SEM Lab
} Smithsonian Institution
} National Museum of Natural History
} PO Box 37012 MRC104
} Washington DC 20013-7012
} 202-633-0891
}
}
}
}
}
} ==============================Original Headers==============================
} 23, 30 -- From connellyps-at-nhlbi.nih.gov Thu Jun 18 19:46:28 2009
} 23, 30 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109])
} 23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5J0kSVr003602
} 23, 30 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 19:46:28 -0500
} 23, 30 -- X-IronPortListener: Outbound_SMTP
} 23, 30 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116])
} 23, 30 -- by nihxwayout.hub.nih.gov with ESMTP; 18 Jun 2009 20:46:28 -0400
} 23, 30 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830);
} 23, 30 -- Thu, 18 Jun 2009 20:46:28 -0400
} 23, 30 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT01.nih.gov
} 23, 30 -- ([156.40.71.20]) with mapi; Thu, 18 Jun 2009 20:46:28 -0400
} 23, 30 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
} 23, 30 -- To: "WHITTAKS-at-si.edu" {WHITTAKS-at-si.edu}
} 23, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 23, 30 -- Date: Thu, 18 Jun 2009 20:44:04 -0400
} 23, 30 -- Subject: Re: [Microscopy] SEM Prep mosquito eggs
} 23, 30 -- Thread-Topic: [Microscopy] SEM Prep mosquito eggs
} 23, 30 -- Thread-Index: AcnwQsIpRM3i1e+hTPKVh5+xSn28xgANElAp
} 23, 30 -- Message-ID: {C6605794.3934%connellyps-at-nhlbi.nih.gov}
} 23, 30 -- In-Reply-To: {200906181829.n5IIThRr018749-at-ns.microscopy.com}
} 23, 30 -- Accept-Language: en, en-US
} 23, 30 -- Content-Language: en
} 23, 30 -- X-MS-Has-Attach:
} 23, 30 -- X-MS-TNEF-Correlator:
} 23, 30 -- acceptlanguage: en, en-US
} 23, 30 -- Content-Type: text/plain; charset="iso-8859-1"
} 23, 30 -- MIME-Version: 1.0
} 23, 30 -- X-OriginalArrivalTime: 19 Jun 2009 00:46:28.0388 (UTC) FILETIME=[61793E40:01C9F077]
} 23, 30 -- Content-Transfer-Encoding: 8bit
} 23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5J0kSVr003602
} ==============================End of - Headers==============================
} .
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400

==============================Original Headers==============================
6, 38 -- From Larry.Ackerman-at-ucsf.edu Fri Jun 19 13:43:00 2009
6, 38 -- Received: from mail.ucsf.edu (bcuda1.ucsf.edu [64.54.247.213])
6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5JIh0NN007160
6, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 13:43:00 -0500
6, 38 -- X-ASG-Debug-ID: 1245436972-5820003a0004-1DjkGe
6, 38 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
6, 38 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
6, 38 -- by mail.ucsf.edu (Spam & Virus Firewall) with ESMTP
6, 38 -- id 6F59823D968; Fri, 19 Jun 2009 11:42:53 -0700 (PDT)
6, 38 -- Received: from EXHT03.net.ucsf.edu ([64.54.247.193]) by mail.ucsf.edu with ESMTP id u0AF5sFnGCJE2Abn; Fri, 19 Jun 2009 11:42:53 -0700 (PDT)
6, 38 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
6, 38 -- Received: from exfe02.net.ucsf.edu (64.54.128.202) by EXHT03.net.ucsf.edu
6, 38 -- (64.54.247.220) with Microsoft SMTP Server id 8.1.375.2; Fri, 19 Jun 2009
6, 38 -- 11:42:50 -0700
6, 38 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by exfe02.net.ucsf.edu
6, 38 -- with Microsoft SMTPSVC(6.0.3790.3959); Fri, 19 Jun 2009 11:42:49 -0700
6, 38 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
6, 38 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Fri, 19 Jun 2009
6, 38 -- 11:42:49 -0700
6, 38 -- Message-ID: {4A3BDC18.4050600-at-ucsf.edu}
6, 38 -- Date: Fri, 19 Jun 2009 11:42:32 -0700
6, 38 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
6, 38 -- Reply-To: larry.ackerman-at-ucsf.edu
6, 38 -- Organization: UCSF, NeuroAnatomy
6, 38 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 38 -- MIME-Version: 1.0
6, 38 -- To: "connellyps-at-nhlbi.nih.gov" {connellyps-at-nhlbi.nih.gov} ,
6, 38 -- Microscopy-at-microscopy.com
6, 38 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: SEM Prep mosquito eggs
6, 38 -- Subject: Re: [Microscopy] Re: SEM Prep mosquito eggs
6, 38 -- References: {200906190053.n5J0rcGH013490-at-ns.microscopy.com}
6, 38 -- In-Reply-To: {200906190053.n5J0rcGH013490-at-ns.microscopy.com}
6, 38 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
6, 38 -- Content-Transfer-Encoding: 8bit
6, 38 -- X-OriginalArrivalTime: 19 Jun 2009 18:42:49.0383 (UTC) FILETIME=[BEBF0B70:01C9F10D]
6, 38 -- X-Barracuda-Connect: UNKNOWN[64.54.247.193]
6, 38 -- X-Barracuda-Start-Time: 1245436973
6, 38 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Fri, 19 Jun 2009 14:51:05 -0500
Subject: [Microscopy] Re: SEM Prep mosquito eggs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We do variations on what both Pat and Larry wrote.

The Nitex in the BEEM capsule is an elegent solution, and we even had one
researcher make them out of the extra-tiny versions. They have lasted for
decades.

We use Ross lens paper instead of Kimwipe, since it seems to shed
less. Our Japanese users make little origami boxes out of the lens tissue,
but we also have the "haole" versions, which is pretty much folding it
over and securing with a staple. For individual mouse eggs (sigh), I made
little origami cup shapes and put them in microporous capsules for CPD.

Right now I have a user on the SEM looking at copepod nauplii that he
managed to keep track of in just the unlined extra-fine microporous
capsules from Pella.

Aloha,
Tina

} Your embryos are about the same size as drosophila embryos which I have
} processed many times for SEM in years past. One of the best embryo
} containers for CPD is one I made and you can easily make. Purchase some
} Nylon or polyethlene (I typically used the Nitex brand of nylon mesh but
} other materials work also) mesh of the appropriate size. I usually
} worked with 80--90 micrometer opening/mesh size. Cut off the bottom of a
} standard Beem embedding capsule so you end up with a cylinder with a
} cap. Cut another cap from another Beem capsule that you will use to cap
} the the second end of the cut Beem capsule cylinder. Cut a little square
} or circle of the Nylon mesh to fit over the end of the Beem capsule with
} extra around the edges. Push a cap on which will seal the mesh to the
} capsule. I processed the tissue in these open at the top Beem capsules
} by placing them, standing up, in a Petri dish filled with fluid and
} changing the fluid as needed. After the final ethanol I added the Nylon
} mesh and cap to the top of the specimen containing Beem capsule. The
} entire capsule or several of them go into the CPD. Fluid transfer is
} quite good through the mesh. When the samples are dry I carefully opened
} one end of the capsule and tapped the dry embryos onto a sample stub
} with one of the various adhesive methods or onto a piece of the very
} smooth Ross lens tissue for careful transfer via forceps.
} There are various commercial assemblies that can utilize a filter made
} with Nylon mesh but I did not find any advantage over Beem capsules.
} Best wishes,
} Larry
}
}
} connellyps-at-nhlbi.nih.gov wrote:
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} }
} } Scott,
} }
} } We have been doing CPD of young mouse embryos. We use a 24 specimen holder
} } (ours from Electron Microscopy Sciences) . The holes are about 4mm in
} } diameter arranged in two discs of 12 holes each with a mesh screen on top
} } and bottom. The outer diameter is about an inch so it fits nicely into our
} } CPD.
} }
} } These small tissues are difficult to get out after the CPD so after fixation
} } we put the holder into water just to the top of the holes, make an envelope
} } or small cup out of a tiny piece of Kimwipe, place it into the holder hole,
} } put or pipet the specimen in then close it up snuggly.
} }
} } To facilitate dehydration (Ethanol) we use two 50 ml Tri-pour beakers fitted
} } with a shelf made of screen bent into a "U" shape with a stir bar
} } underneath. The level of ethanol is such that when the specimen holder is
} } put into the beaker it will be well covered. Put the beaker on a stirrer
} } for as long as you like. Alternate between the two beakers giving a rinse
} } with the next solution before filling. I have not had amyl acetate in a
} } Tri-pour beaker - you'd know if you would need to use glass.
} }
} } After CPD the tissues come easily out of the holder by grabbing the paper.
} } The specimen does not get touched. You could "dump" the egg/eggs right onto
} } the prepped SEM stub without needing to touch them.
} }
} } Depending on your samples you may be able to do them all in a few runs.
} }
} } Second thought...
} } Years ago I worked with rabbit eggs and needed to use 1% Acrolein in my
} } fixative (glutaraldehyde plus paraformaldehyde) to get a good fix for TEM.
} } Would that help? If so be advised that acrolein is in tear gas or it acts
} } like it!
} } Since the eggs are laid on water, do you use water or buffer for your fix?
} }
} } Pat
} }
} } Patricia Stranen Connelly
} } Research Assistant
} } NHLBI Electron Microscopy Core
} } National Institutes of Health
} } 14 Service Road West
} } Bldg. 14E ­ Rm. 111B MSC 5570
} } Bethesda, MD 20892-5570
} } Phone 301-496-3491
} } FAX 301-480-6560
} } connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} }
} } Opinions and experiences related are those of Pat Connelly and do not
} } represent the NIH. This message is not confidential and can be freely shared
} } and reproduced.
} }
} } =======================
} } X-from: {WHITTAKS-at-si.edu}
} } Reply-To: {WHITTAKS-at-si.edu}
} } Date: Thu, 18 Jun 2009 14:29:43 -0400
} } To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
} } Conversation: [Microscopy] SEM Prep mosquito eggs
} } Subject: [Microscopy] SEM Prep mosquito eggs
} } ----------------------------------------------------------------------------
} }
} } All,
} }
} } I have a researcher here who is studying mosquito eggs. They are 100x300 µm
} } and have an extremely delicate membrane surrounding them. I seem to only get
} } good preservation via CPD out of amyl acetate. Problem is getting enough
} } samples in the chamber to be useful. I received 30 last time and it took a
} } week and a half to prepare them all as I can only get 3 samples in the CPD
} } chamber using the filter membrane holders- and it uses A LOT of expensive
} } solvent.
} }
} } I just found out I have about 300 more samples coming and can expect this
} } numerous times over the next year or so. The look I got when I glanced at my
} } volunteer as she slunk away was priceless. Have any of you practical
} } experience with preparing samples this small yet in quantity? I have a few
} } ideas and will test them out but no sense in reinventing the wheel.
} }
} } One thought I had was using glass pipette tips, suck them in and plug with
} } something permeable(agarose maybe or would it shrink too much and fall out).
} }
} } Another is little bags of dialysis tubing with them floating around inside.
} } Is amyl acetate too big to exchange?
} }
} } Yet another is some very small glass vials with TEM grids glued on the end.
} } However glued on is difficult to open. Anything less permanent yet solvent
} } resistant?
} }
} } With either of these I could do at least 30 a day but wanted to poll the
} } list for less theoretical contraptions.
} }
} } Scott Whittaker
} } Head NMNH Imaging
} } Manager SEM Lab
} } Smithsonian Institution
} } National Museum of Natural History
} } PO Box 37012 MRC104
} } Washington DC 20013-7012
} } 202-633-0891
} }
} }
} }
} }
} }
} } ==============================Original Headers==============================
} } 23, 30 -- From connellyps-at-nhlbi.nih.gov Thu Jun 18 19:46:28 2009
} } 23, 30 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109])
} } 23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5J0kSVr003602
} } 23, 30 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2009 19:46:28 -0500
} } 23, 30 -- X-IronPortListener: Outbound_SMTP
} } 23, 30 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116])
} } 23, 30 -- by nihxwayout.hub.nih.gov with ESMTP; 18 Jun 2009 20:46:28 -0400
} } 23, 30 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830);
} } 23, 30 -- Thu, 18 Jun 2009 20:46:28 -0400
} } 23, 30 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT01.nih.gov
} } 23, 30 -- ([156.40.71.20]) with mapi; Thu, 18 Jun 2009 20:46:28 -0400
} } 23, 30 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
} } 23, 30 -- To: "WHITTAKS-at-si.edu" {WHITTAKS-at-si.edu}
} } 23, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } 23, 30 -- Date: Thu, 18 Jun 2009 20:44:04 -0400
} } 23, 30 -- Subject: Re: [Microscopy] SEM Prep mosquito eggs
} } 23, 30 -- Thread-Topic: [Microscopy] SEM Prep mosquito eggs
} } 23, 30 -- Thread-Index: AcnwQsIpRM3i1e+hTPKVh5+xSn28xgANElAp
} } 23, 30 -- Message-ID: {C6605794.3934%connellyps-at-nhlbi.nih.gov}
} } 23, 30 -- In-Reply-To: {200906181829.n5IIThRr018749-at-ns.microscopy.com}
} } 23, 30 -- Accept-Language: en, en-US
} } 23, 30 -- Content-Language: en
} } 23, 30 -- X-MS-Has-Attach:
} } 23, 30 -- X-MS-TNEF-Correlator:
} } 23, 30 -- acceptlanguage: en, en-US
} } 23, 30 -- Content-Type: text/plain; charset="iso-8859-1"
} } 23, 30 -- MIME-Version: 1.0
} } 23, 30 -- X-OriginalArrivalTime: 19 Jun 2009 00:46:28.0388 (UTC) FILETIME=[61793E40:01C9F077]
} } 23, 30 -- Content-Transfer-Encoding: 8bit
} } 23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5J0kSVr003602
} } ==============================End of - Headers==============================
} } .
} }
}
} --
} Larry Ackerman, Specialist
} UCSF, Dept. of Anatomy, Rm S1347
} 513 Parnassus Ave., Box 0452
} San Francisco, CA 94143
}
} larry.ackerman-at-ucsf.edu
}
} 415-476-4400
}
} ==============================Original Headers==============================
} 6, 38 -- From Larry.Ackerman-at-ucsf.edu Fri Jun 19 13:43:00 2009
} 6, 38 -- Received: from mail.ucsf.edu (bcuda1.ucsf.edu [64.54.247.213])
} 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5JIh0NN007160
} 6, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 13:43:00 -0500
} 6, 38 -- X-ASG-Debug-ID: 1245436972-5820003a0004-1DjkGe
} 6, 38 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
} 6, 38 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
} 6, 38 -- by mail.ucsf.edu (Spam & Virus Firewall) with ESMTP
} 6, 38 -- id 6F59823D968; Fri, 19 Jun 2009 11:42:53 -0700 (PDT)
} 6, 38 -- Received: from EXHT03.net.ucsf.edu ([64.54.247.193]) by mail.ucsf.edu with ESMTP id u0AF5sFnGCJE2Abn; Fri, 19 Jun 2009 11:42:53 -0700 (PDT)
} 6, 38 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
} 6, 38 -- Received: from exfe02.net.ucsf.edu (64.54.128.202) by EXHT03.net.ucsf.edu
} 6, 38 -- (64.54.247.220) with Microsoft SMTP Server id 8.1.375.2; Fri, 19 Jun 2009
} 6, 38 -- 11:42:50 -0700
} 6, 38 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by exfe02.net.ucsf.edu
} 6, 38 -- with Microsoft SMTPSVC(6.0.3790.3959); Fri, 19 Jun 2009 11:42:49 -0700
} 6, 38 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by
} 6, 38 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Fri, 19 Jun 2009
} 6, 38 -- 11:42:49 -0700
} 6, 38 -- Message-ID: {4A3BDC18.4050600-at-ucsf.edu}
} 6, 38 -- Date: Fri, 19 Jun 2009 11:42:32 -0700
} 6, 38 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
} 6, 38 -- Reply-To: larry.ackerman-at-ucsf.edu
} 6, 38 -- Organization: UCSF, NeuroAnatomy
} 6, 38 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
} 6, 38 -- MIME-Version: 1.0
} 6, 38 -- To: "connellyps-at-nhlbi.nih.gov" {connellyps-at-nhlbi.nih.gov} ,
} 6, 38 -- Microscopy-at-microscopy.com
} 6, 38 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: SEM Prep mosquito eggs
} 6, 38 -- Subject: Re: [Microscopy] Re: SEM Prep mosquito eggs
} 6, 38 -- References: {200906190053.n5J0rcGH013490-at-ns.microscopy.com}
} 6, 38 -- In-Reply-To: {200906190053.n5J0rcGH013490-at-ns.microscopy.com}
} 6, 38 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
} 6, 38 -- Content-Transfer-Encoding: 8bit
} 6, 38 -- X-OriginalArrivalTime: 19 Jun 2009 18:42:49.0383 (UTC) FILETIME=[BEBF0B70:01C9F10D]
} 6, 38 -- X-Barracuda-Connect: UNKNOWN[64.54.247.193]
} 6, 38 -- X-Barracuda-Start-Time: 1245436973
} 6, 38 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



==============================Original Headers==============================
9, 22 -- From tina-at-pbrc.hawaii.edu Fri Jun 19 14:51:04 2009
9, 22 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5JJp3DB023621
9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 14:51:04 -0500
9, 22 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
9, 22 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n5JJow9H001206
9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 09:50:59 -1000 (HST)
9, 22 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n5JJowAY001203
9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 09:50:58 -1000 (HST)
9, 22 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
9, 22 -- Date: Fri, 19 Jun 2009 09:50:57 -1000 (HST)
9, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
9, 22 -- X-Sender: tina-at-halia
9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 22 -- Subject: Re: [Microscopy] SEM Prep mosquito eggs
9, 22 -- In-Reply-To: {200906191844.n5JIiOMo008618-at-ns.microscopy.com}
9, 22 -- Message-ID: {Pine.GSO.4.21.0906190944260.992-100000-at-halia}
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: TEXT/PLAIN; charset=X-UNKNOWN
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n5JJp3DB023621
==============================End of - Headers==============================




From: Jessica.Nims-at-pnl.gov
Date: Fri, 19 Jun 2009 16:04:28 -0500
Subject: [Microscopy] Electron Microscopist Opportunity at a DOE National Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello!

We have an electron microscopy position open here at the Pacific Northwest National Laboratory. If you are interested - or know someone who might be - please follow the instructions below to find out more or to apply online. The job description is also below.

- Go to "jobs.pnl.gov"
- Click "Current Job Openings"
- Click "Advanced Search"
- Enter 117594 into the "Job ID" field

Feel free to contact me if you have any questions.

Thanks!

Jessica
__________________________________________________
Jessica Nims
National Security Recruiting Specialist
Pacific Northwest National Laboratory
Tel:  509-375-3869
Fax: 509-372-4301
jessica.nims-at-pnl.gov



Electron Microscopist

Please apply online at jobs.pnl.gov for job 117594.

This position is located in Richland, WA.

Company Description
The Pacific Northwest National Laboratory, located in Richland, WA, is one of the U.S. Department of Energy's (DOE's) national laboratories, managed by DOE's Office of Science. PNNL delivers breakthrough science and technology to meet today's key national needs. Please visit our website - www.pnl.gov - for more information.

Technical Group Description
PNNL offers world-class radio analytical capabilities that significantly contribute to the security of the United States and the world. Our staff's expertise includes characterization of particulate materials and mass spectrometry. Our staff uses state-of-the-art equipment to perform work that spans the spectrum of basic science to unique analytical services.
Position Description
This position will provide electron microscopy analysis for characterization of geologic and man-made particulate materials for an ongoing analytical production project. In addition, this position will be part of R&D projects that include particulate characterization by SEM, EMP, TEM, EBSD and XRD. The incumbent will work both independently and in collaboration with project teams, the position will support a range of US Government and private clients. The research group seeking to fill this position supports a number of critical national security and homeland security missions along with missions relating to environmental monitoring. This position will report to one or more task leaders on the project team as well as to the line manager (team lead) of the technical group.

- Level I: Performs basic science and engineering tasks and activities with minimal oversight. Primary focus is on application of capabilities to specific tasks assigned by project leaders with some independent work expectations.

- Level II: Independently completes recurring assignments. Exercises limited judgment on work details. Makes preliminary selections and adaptations of technical alternatives. Expected to contribute professionally, building a professional reputation for technical expertise. Fully applying and interpreting standard theories, principles, methods, tools and technologies.

Will provide support to the Advanced Radioanalytical Chemistry group within the National Security Division. Will work directly with multidisciplinary project teams to characterize particulate materials using electron microscopy tools. Will operate scanning electron microscopes and electron microprobes, perform imaging, as well as energy and wave-length dispersive analysis. Will assume partial responsibility for the equipment and lab space as an alternative laboratory cognizant space manager. The expected outcome of the staff member's contribution to projects will be timely delivery of microscopy data and reports to task leads and project managers working in the group.

Initially, work will be directed by senior team members. However as the staff member develops, it is expected that he/she will become more involved in assuming direct responsibility for specific project tasks.


Position Requirements

Technical Expertise: Experience should be in scanning electron microscopy and/or electron microprobe analysis. Experience in transmission electron microscopy, x-ray diffraction, electron backscatter detection or other analytical techniques is desired.

Level of Responsibility: Strong interpersonal skills and the ability to collaborate effectively on diverse teams are required. Candidates should be creative, and capable of productive research both independently and as part of diverse teams.

Breadth of Relevant Technical Knowledge: The successful candidate should be good at problem solving, have meticulous attention to detail, and an in-depth knowledge of electron microscope operation. Other desirable experience includes familiarity with spread sheets, word processing software, and image analysis software. Due to the multidisciplinary nature of the work and project teams, a fundamental grasp of basic mineralogy, material science, or chemistry principles is essential.

Minimum Education/Experience:
- Level I: A minimum of a Bachelor's degree with 0-1 years experience. Expected to contribute professionally, applying basic theories, principles, methods, tools and technologies to well-defined assignments of limited scope.
- Level II: BS + 4 years of experience in process analytical work. Masters degree preferred. Demonstrated proficiency with electron microscopy tools, ability to work independently with platforms.

Clearance Level and/or U.S. Citizenship Required: Ability to obtain and maintain a DOE Q Security clearance (to obtain a clearance requires U.S. Citizenship).


__________________________

Pacific Northwest National Laboratory (PNNL) is an Affirmative Action / Equal Opportunity Employer and supports diversity in the workplace. Applicants will be considered for employment without regard to race, color, religion, sex, national origin, age, disability, veteran status, marital status, or sexual orientation.

Applicants selected will be subject to a Federal background investigation and must meet eligibility requirements for access to classified matter in accordance 10 CFR 710, Appendix B. All Security Clearance (L or Q) positions will be considered by the Department of Energy to be Testing Designated Positions which means that they are subject to applicant, random, and for cause drug testing. In addition, applicants must be able to demonstrate non-use of illegal drugs for the 12 consecutive months preceding completion of the requisite Questionnaire for National Security Positions (QNSP).

Note: Applicants will be considered ineligible for security clearance processing by the U.S. Department of Energy until non-use of illegal drugs for 12 consecutive months can be demonstrated.


==============================Original Headers==============================
30, 29 -- From prvs=414c4250d=Jessica.Nims-at-pnl.gov Fri Jun 19 16:04:28 2009
30, 29 -- Received: from emailgw04.pnl.gov (emailgw04.pnl.gov [192.101.109.35])
30, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5JL4RVk007228
30, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jun 2009 16:04:27 -0500
30, 29 -- X-IronPort-AV: E=Sophos;i="4.42,255,1243839600";
30, 29 -- d="scan'208";a="5729941"
30, 29 -- Received: from emailbh02.pnl.gov ([130.20.128.50])
30, 29 -- by emailgw04.pnl.gov with ESMTP; 19 Jun 2009 14:04:27 -0700
30, 29 -- Received: from EMAIL03.pnl.gov ([130.20.128.72]) by emailbh02.pnl.gov with Microsoft SMTPSVC(6.0.3790.3959);
30, 29 -- Fri, 19 Jun 2009 14:04:27 -0700
30, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
30, 29 -- MIME-Version: 1.0
30, 29 -- Content-Type: text/plain;
30, 29 -- charset="iso-8859-1"
30, 29 -- x-cr-puzzleid: {3BDD8EFB-7372-4009-806F-D6307E173288}
30, 29 -- x-cr-hashedpuzzle: Arnb CwO7 CzzZ Ewvj FKmm G5jx Hi2A IQJH Ive/ IxGR I2Sg KSit LAfh LIFW L/NY MFAR;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{3BDD8EFB-7372-4009-806F-D6307E173288};agBlAHMAcwBpAGMAYQAuAG4AaQBtAHMAQABwAG4AbAAuAGcAbwB2AA==;Fri, 19 Jun 2009 21:04:23 GMT;RQBsAGUAYwB0AHIAbwBuACAATQBpAGMAcgBvAHMAYwBvAHAAaQBzAHQAIABPAHAAcABvAHIAdAB1AG4AaQB0AHkAIABhAHQAIABhACAARABPAEUAIABOAGEAdABpAG8AbgBhAGwAIABMAGEAYgBvAHIAYQB0AG8AcgB5AA==
30, 29 -- Content-class: urn:content-classes:message
30, 29 -- Subject: Electron Microscopist Opportunity at a DOE National Laboratory
30, 29 -- Date: Fri, 19 Jun 2009 14:04:23 -0700
30, 29 -- Message-ID: {77ABE1C6F7B0AE4794A66EBAB92B1F6B01A419B1-at-EMAIL03.pnl.gov}
30, 29 -- X-MS-Has-Attach:
30, 29 -- X-MS-TNEF-Correlator:
30, 29 -- Thread-Topic: Electron Microscopist Opportunity at a DOE National Laboratory
30, 29 -- Thread-Index: AcnxIYV/f/Wd76NfR3ucqo30LMYnrQ==
30, 29 -- From: "Nims, Jessica A" {Jessica.Nims-at-pnl.gov}
30, 29 -- To: {Microscopy-at-microscopy.com}
30, 29 -- X-OriginalArrivalTime: 19 Jun 2009 21:04:27.0389 (UTC) FILETIME=[87F3DAD0:01C9F121]
30, 29 -- Content-Transfer-Encoding: 8bit
30, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5JL4RVk007228
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Mon, 22 Jun 2009 07:52:03 -0500
Subject: [Microscopy] Microscopy Today July 2009 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Microscopy Today has a new editor-in-chief, a new look and format, and a
new digital edition. Appended below is the July editorial by Charles
Lyman and the July Table of Contents.

MT remains a free publication in print form for MSA members and
microscopists in North America. New subscriptions, address changes, or
unsubscribe requests should be directed to http://www.microscopy-today.com

Ron Anderson

=======================================================

* Editorial*

Welcome to the new look of Microscopy Today, the magazine for all
microscopists and microanalysts. This controlled-circulation publication
is still owned by the Microscopy Society of America, but it is now
published by Cambridge University Press along with the society’s
peer-reviewed scientific journal, Microscopy and Microanalysis.

How was Microscopy Today published before this issue? Answer: through
Ron Anderson’s editorial skill, financial acumen, and determined
salesmanship. While Ron handled most editorial and production tasks
single-handedly, he was ably assisted by Dale Anderson (Art Director),
Phil Oshel (Technical Editor), and Renée Stratmoen (Advertising
Director). Without seven successful years under their leadership, MT
might not even be an MSA publication. Thanks, Ron.

What is different inside these covers? Many features remain the same:
Stephen Carmichael’s column (his 109th appears in this issue), NetNotes
(edited by Thomas Phillips), Dear Abbe (correspondence handled by John
Shields), Microscopy 101, and eight-to-ten articles covering all areas
of microscopy. New aspects include a feature article that provides an
overview of a topic of interest to practicing microscopists (MSA
President-Elect Dave Piston supplied the feature article for this
issue), a new column entitled Microscopy Pioneers (edited by Michael
Davidson), and a column about Microscopy Education (edited by Steven
Barlow).

A completely new feature is the Microscopy Today digital edition.
Beginning with this issue, MT will be published simultaneously as an
exact-replica digital edition. You may have seen other digital
magazines, but MT’s on-line presence will have a special feature. The
reader will be able to adjust the page magnification continuously,
overcoming an annoying difficulty with many digital magazines. Try it
out at the MT website: www.microscopy-today.com. (Available simultaneous
with the printed edition shortly).

Finally, I am announcing some new awards. Microscopy Today is sponsoring
ten awards for the most innovative instruments or methods related to
microscopy in a given year. The awards will be called the MT-10 Awards.
Details about the submission process and deadlines will be given in the
September issue.

Charles Lyman Editor-in-Chief

--------------------------------------

*Table of Contents*

* Feature Article*

Photoactivation in Fluorescence Microscopy
David W. Piston, Gert-Jan Kremers, Richard K.P. Benninger, and Michael
W. Davidson

*Biological Applications*

The Long Shot: Multiphoton Microscopy Offers Deeper, Sharper, Safer
Imaging Than Ever Before
Sam Tesfai, John Jordan, and Dennis Donley

Red/green Dual Fluorescence Detection of Both the Nucleus and Nucleolus
in Living Cells
Jack Coleman, Hilary Cox, Zaiguo Li, Praveen Pande, Dee Shen, Divina
Gatica, and Wayne F. Patton

*Materials Applications*

Interface Stabilized Nanoscale Quasi-Liquid Films
Jian Luo, Shen J. Dillon, and Martin P. Harmer

SEM-EDX at the Service of Archaeology to Unravel Historical Technology
Carolina Cardell, Isabel Guerra, and Antonio Sánchez-Navas

*Instrumentation*

Applications of Multibeam SEM/FIB Instrumentation in the Integrated Sciences
G. McMahon, J. Rybczynski, Y. Wang, Y. Gao, D. Cai, P. Dhakal, N. Argenti,
K. Kempa, Z.F. Ren, N. Erdman, and M.J. Naughton

Improving SEM Imaging Performance Using Beam Deceleration
D. Phifer, L.Tuma, T. Vystavel, P. Wandrol, and R.J. Young

A Novel Heating Technology for Ultra-High Resolution Imaging in Electron
Microscopes
Lawrence F. Allard, Wilbur C. Bigelow, Steven A. Bradley, and Jingyue
(Jimmy) Liu

Innovative Instrumentation for Analysis of Nanoparticles: The ?
Steradian Detector
Nestor J. Zaluzec

*Departments*

Editorial
Charmichael’s Concise Review
Microscopy Pioneers
Microscopy Education
Microscopy 101
Industry News
NetNotes
Dear Abbe
Opinion
Index of Advertisers





==============================Original Headers==============================
33, 17 -- From randerson20-at-tampabay.rr.com Mon Jun 22 07:52:03 2009
33, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
33, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5MCq2Qf010936
33, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 22 Jun 2009 07:52:03 -0500
33, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
33, 17 -- by hrndva-omta03.mail.rr.com with ESMTP
33, 17 -- id {20090622125201446.SVLO85-at-hrndva-omta03.mail.rr.com}
33, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 22 Jun 2009 12:52:01 +0000
33, 17 -- Message-ID: {4A3F7E6F.1030104-at-tampabay.rr.com}
33, 17 -- Date: Mon, 22 Jun 2009 08:51:59 -0400
33, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
33, 17 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
33, 17 -- MIME-Version: 1.0
33, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
33, 17 -- Subject: Microscopy Today July 2009 Table of Contents
33, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
33, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: lang-at-eps.mcgill.ca
Date: Mon, 22 Jun 2009 11:11:06 -0500
Subject: [Microscopy] Sdd vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Members,

I would like to have the comments on Silicon Drift Detector (SDD), especially
advantages and disadvantages compared with SiLi detector.

Thanks.

Lang
EPS, McGill University



----------------------------------------------------------------
This message was sent using IMP, the Internet Messaging Program.

==============================Original Headers==============================
8, 30 -- From lang-at-eps.mcgill.ca Mon Jun 22 11:11:06 2009
8, 30 -- Received: from drizzle.cc.mcgill.ca (drizzle.cc.mcgill.ca [132.206.27.48])
8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5MGB50S000537
8, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Jun 2009 11:11:06 -0500
8, 30 -- Received: from mailscan1.ncs.mcgill.ca (mailscan1.NCS.McGill.CA [132.216.77.248])
8, 30 -- by drizzle.cc.mcgill.ca (8.12.11.20060308/8.12.3) with ESMTP id n5MGB5qq026924
8, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Jun 2009 12:11:05 -0400
8, 30 -- Received: from mailscan1.ncs.mcgill.ca (localhost [127.0.0.1])
8, 30 -- by localhost (Postfix) with SMTP id 8EB207D7D
8, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Jun 2009 12:11:05 -0400 (EDT)
8, 30 -- Received: from cobbles.eps.mcgill.ca (cobbles.eps.mcgill.ca [132.206.152.2])
8, 30 -- by mailscan1.ncs.mcgill.ca (Postfix) with ESMTP id 77B5D7D7C
8, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Jun 2009 12:11:05 -0400 (EDT)
8, 30 -- Received: by cobbles.eps.mcgill.ca (Postfix, from userid 99)
8, 30 -- id 66FD0231CC3; Mon, 22 Jun 2009 12:11:05 -0400 (EDT)
8, 30 -- Received: from ip166.eps.mcgill.ca (ip166.eps.mcgill.ca [132.206.152.166])
8, 30 -- by www.eps.mcgill.ca (IMP) with HTTP
8, 30 -- for {lang-at-localhost} ; Mon, 22 Jun 2009 12:11:05 -0400
8, 30 -- Message-ID: {1245687065.4a3fad195f047-at-www.eps.mcgill.ca}
8, 30 -- Date: Mon, 22 Jun 2009 12:11:05 -0400
8, 30 -- From: lang-at-eps.mcgill.ca
8, 30 -- To: Microscopy-at-microscopy.com
8, 30 -- Subject: Sdd vs SiLi
8, 30 -- MIME-Version: 1.0
8, 30 -- Content-Type: text/plain; charset=ISO-8859-1
8, 30 -- Content-Transfer-Encoding: 8bit
8, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.4
8, 30 -- X-Originating-IP: 132.206.152.166
8, 30 -- X-PMX-Version: 5.4.2.338381, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.6.15.190726
8, 30 -- X-McGill-WhereFrom: Internal
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Tue, 23 Jun 2009 09:54:54 -0500
Subject: [Microscopy] Nikon coolpix for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
We have a Nikon Coolpix 990 that we use for imaging on a light
microscope. It is easy to use and the images look great. I've had a
researcher request new camera info - they want the same sort of set up
for LM. Is there a favorite new camera out there for LM?
Is anyone using the new Coolpix P90 for LM?

Your advice is greatly appreciated.
thanks,
Beth


==============================Original Headers==============================
3, 19 -- From beth-at-plantbio.uga.edu Tue Jun 23 09:54:52 2009
3, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5NEspQI021918
3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Jun 2009 09:54:51 -0500
3, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
3, 19 -- (authenticated user beth-at-plantbio.uga.edu)
3, 19 -- by dogwood.plantbio.uga.edu
3, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
3, 19 -- for microscopy-at-microscopy.com;
3, 19 -- Tue, 23 Jun 2009 10:54:47 -0400
3, 19 -- Message-Id: {36C57FAD-0D83-4A94-AC9D-CD91A6900725-at-plantbio.uga.edu}
3, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
3, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
3, 19 -- Content-Transfer-Encoding: 7bit
3, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3)
3, 19 -- Subject: Nikon coolpix for LM
3, 19 -- Date: Tue, 23 Jun 2009 10:54:49 -0400
3, 19 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: karsten.goemann-at-utas.edu.au
Date: Tue, 23 Jun 2009 19:53:22 -0500
Subject: [Microscopy] Lab analyst position EPMA/SEM/uXRF Uni of Tasmania

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

please see below for a position re-opening at
University of Tasmania in Hobart for a Laboratory
Analyst in the area of scanning electron
microscopy and x-ray microanalysis.
We advertised for a slightly different position
end of last year and hired a laser ablation
specialist who now works full time in our laser
ablation facility. Work kept increasing and we're
still looking for another full-time staff member
to work with me in the electron microscopy /
x-ray microanalysis area. Position description
and salary package have changed slightly.

Apologies in advance if you receive this post
several times due to cross-posting to other lists.

Thanks and cheers,
Karsten

Reference No: HG 171/09
Position: Laboratory Analyst, SEM, EPMA
Type: General
Location: Hobart
School/Section: CODES
Faculty/Division: Faculty of Science, Engineering & Technology
Appointment: Fixed-Term
Availability: Internal & External
Closing Date: Monday, 20 July 2009
Applications are invited for appointment to this
position, which will be offered on a full-time
fixed-term basis, commencing on or after 1
September 2009 for a period of 24 months, with a
possibility of extension for up to 4.5 years.
CODES is a Centre of Excellence funded by the
Australian Research Council, which undertakes
research focused on the origin and discovery of
ore deposits. The Centre currently employs over
30 academic and 7 general staff and has a large
group of postgraduate students. The Centre is
located within the Sandy Bay Campus of the
University.
The position will involve active participation
running the Electron Microscopy and X-ray
Microanalysis Facility of the Central Science
Laboratory (CSL), which is currently housing
three major instruments: A FEI Quanta 600 MLA
scanning electron microscope (SEM), a Cameca
SX100 electron microprobe (EPMA), and a Horiba
XGT-7000V x-ray analytical microscope (µXRF). The
appointee will undertake maintenance of the
analytical instruments, provide expert advice on
the application of the different instruments to
geological problems, demonstrate their operation
to staff and students and develop further
analytical techniques for sample analyses.
The successful applicant will have a honours
degree qualification in earth sciences with a
substantial geochemical component and with at
least 2 years analytical lab-based experience, or
a postgraduate degree (Masters/PhD) in
geochemistry. The successful applicant will also
have demonstrated experience in working with
analytical equipment, interest and the ability to
show initiative in analytical developments,
experience with data reduction procedures,
experience in the interpretation of geochemical
microanalysis data and well-developed
organisational, and written and oral
communication skills.
The appointment will be at HEO Level 7 and will
have a total remuneration package of up to
$83,673 per annum. This package comprises salary
within the range $64,217 - $71,515 plus 17%
superannuation, with the option of an additional
3% salary loading in exchange for 14% instead of
17% superannuation.
For further information about the position please
contact Dr Leonid Danyushevsky, Leader, CODES
Technology Research Program on telephone (03)
6226 2469, fax (03) 6226 7662 or email
L.Dan-at-utas.edu.au.
The University is an equal opportunity Employer.

To access the job application package including a
full position description, go to the following
URL:
http://jobs.admin.utas.edu.au/positions/pd_detail.aspx?PositionId=1079


--
Dr. Karsten Goemann
Scientific Officer-in-Charge, Electron Microscopy & X-ray Microanalysis
Central Science Laboratory, University of Tasmania
Private Bag 74, Hobart TAS 7001, Australia
Tel.: +61 (0) 3 6226 2146
Fax: +61 (0) 3 6226 2494
E-mail: Karsten.Goemann-at-utas.edu.au
http://www.utas.edu.au/csl


==============================Original Headers==============================
9, 17 -- From karsten.goemann-at-utas.edu.au Tue Jun 23 19:53:22 2009
9, 17 -- Received: from corinna.its.utas.edu.au (corinna.its.utas.edu.au [131.217.10.51])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5O0rLPg006889
9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 23 Jun 2009 19:53:21 -0500
9, 17 -- Received: from [131.217.50.60] (kgoemann.csl.utas.edu.au [131.217.50.60])
9, 17 -- by corinna.its.utas.edu.au (8.13.8+Sun/8.13.6) with ESMTP id n5O0rI7n011063;
9, 17 -- Wed, 24 Jun 2009 10:53:19 +1000 (EST)
9, 17 -- Mime-Version: 1.0
9, 17 -- Message-Id: {f06230907c66727a092b6-at-[131.217.50.60]}
9, 17 -- Date: Wed, 24 Jun 2009 10:53:18 +1000
9, 17 -- To: microscopy-at-microscopy.com
9, 17 -- From: Karsten Goemann {karsten.goemann-at-utas.edu.au}
9, 17 -- Subject: Lab analyst position EPMA/SEM/uXRF Uni of Tasmania
9, 17 -- Cc: l.dan-at-utas.edu.au
9, 17 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
9, 17 -- Content-Transfer-Encoding: 8bit
9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5O0rLPg006889
==============================End of - Headers==============================




From: i.white-at-ucl.ac.uk
Date: Wed, 24 Jun 2009 17:43:30 -0500
Subject: [Microscopy] viaWWW: Distance between gold and antigen in Tokuyasu staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both i.white-at-ucl.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: i.white-at-ucl.ac.uk
Name: Ian White

Organization: MRC Unit at University College London

Title-Subject: [Filtered] Distance between gold and antigen in
Tokuyasu staining

Question: If I label an antigen on a section using the Tokuyasu
technique with a primary IgG, and then a bridging IgG before finally
labelling this with protein A gold, what in approximate theoretical
terms is the maximum distance my gold partical can be from the
antigen it is "labelling"?

In other words, how long is IgG+IgG+proteinA?

Thanks for any help

Login Host: 128.40.81.24
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Jun 24 17:43:29 2009
8, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5OMhNwJ019936
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009 17:43:25 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6685c793d60-at-[206.69.208.22]}
8, 11 -- Date: Wed, 24 Jun 2009 17:43:22 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: i.white-at-ucl.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Distance between gold and antigen in Tokuyasu staining
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tgreco-at-marine.usf.edu
Date: Wed, 24 Jun 2009 17:43:55 -0500
Subject: [Microscopy] viaWWW: SDD vs. SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tgreco-at-marine.usf.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tgreco-at-marine.usf.edu
Name: Tony Greco

Organization: USF College of Marine Science

Title-Subject: [Filtered] SDD vs. SiLi

Question: There really is no reason to purchase a SiLi detector as
the Silicon Drift detectors now have vastly superior count rates,
better resolution and excellent low end peak separation. SiLi
detectors offer no advantage at all.

Login Host: 131.247.139.233
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Jun 24 17:43:53 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5OMhpFL020109
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009 17:43:52 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6685c9042a1-at-[206.69.208.22]}
6, 11 -- Date: Wed, 24 Jun 2009 17:43:50 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: tgreco-at-marine.usf.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: SDD vs. SiLi
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Henning.Stahlberg-at-unibas.ch
Date: Wed, 24 Jun 2009 17:44:30 -0500
Subject: [Microscopy] viaWWW: Image Processing positions at the University Basel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Henning.Stahlberg-at-unibas.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Henning.Stahlberg-at-unibas.ch
Name: Henning Stahlberg

Organization: Biozentrum, University Basel, Switzerland

Title-Subject: [Filtered] Image Processing positions at the University Basel

Question:
PhD, Postdoctoral or Senior Scientist Positions Available
in Image Processing and/or Software Development at the University Basel
Center for Cellular Imaging and Nano Analytics (C-CINA)
Biozentrum, University Basel, Switzerland

The Center for Cellular Imaging and Nano Analytics is studying
several biological systems by cryo electron tomography, single
particle cryo-EM and electron crystallography. The lab is excellently
equipped, housing among other instruments a FEI Titan Krios. Projects
include the imaging of cellular proteomes with a single
particle/electron tomography approach, and the study of the
structure, function, and complex formation of membrane proteins,
secretion systems, and DNA-binding protein complexes. I recently
moved from UC Davis to the University Basel. We are now setting up a
new research group in Basel, and have several open positions in the
areas of cryo-EM imaging, image processing, and software development.

We are looking for individuals to participate in the image processing
of electron tomography and single particle cryo-EM data. Experience
with single particle processing (e.g. SPIDER, Frealign, etc.) and/or
electron tomography 3D reconstruction (e.g. iMod, UCSF tomography, or
TOM) would be welcome. In addition, we are looking for a members of
our software development team. Available projects concern the testing
and implementation of new algorithms for electron crystallography and
electron tomography image processing, the maintenance and further
development of electron crystallography software (see 2dx.org), and
the development of a software module for the refinement of tomography
3D reconstructions.

The positions are funded for several years.

If you are interested, or for further information, please contact me at:

Henning Stahlberg
Center for Cellular Imaging and Nanoanalytics (C-CINA)
Structural Biology and Biophysics, Biozentrum,
WRO-1058, Mattenstrasse 26
University Basel, CH-4058 Basel, Switzerland
Tel: +41-61-387 32 62 (office)
Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
Fax: +41-61-387 39 86
mailto:Henning.Stahlberg-at-unibas.ch
http://stahlberglab.org
http://2dx.org


Login Host: 129.132.128.96
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Wed Jun 24 17:44:29 2009
12, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5OMiPXs020657
12, 11 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009 17:44:27 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240802c6685caf49f1-at-[206.69.208.22]}
12, 11 -- Date: Wed, 24 Jun 2009 17:44:23 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: Henning.Stahlberg-at-unibas.ch (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Image Processing positions at the University Basel
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Wed, 24 Jun 2009 19:12:47 -0500
Subject: [Microscopy] Re: viaWWW: Distance between gold and antigen in Tokuyasu staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ian,

the archives of this listserver have a number of messages dealing with
your question. Just in case these are accessible for you, I have sent
a collection to your email address.

cheers

Jan

On 25/06/2009, at 10:44 AM, i.white-at-ucl.ac.uk wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both i.white-at-ucl.ac.uk as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: i.white-at-ucl.ac.uk
} Name: Ian White
}
} Organization: MRC Unit at University College London
}
} Title-Subject: [Filtered] Distance between gold and antigen in
} Tokuyasu staining
}
} Question: If I label an antigen on a section using the Tokuyasu
} technique with a primary IgG, and then a bridging IgG before finally
} labelling this with protein A gold, what in approximate theoretical
} terms is the maximum distance my gold partical can be from the
} antigen it is "labelling"?
}
} In other words, how long is IgG+IgG+proteinA?
}
} Thanks for any help
}
} Login Host: 128.40.81.24


==============================Original Headers==============================
7, 19 -- From leunissen-at-aurion.nl Wed Jun 24 19:12:47 2009
7, 19 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244])
7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5P0CkFJ011456
7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009 19:12:46 -0500
7, 19 -- Received: from [192.168.1.50] (really [222.152.98.164]) by fep02.xtra.co.nz
7, 19 -- with ESMTP
7, 19 -- id {20090625001240.ZSDU28374.fep02.xtra.co.nz-at-[192.168.1.50]}
7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 12:12:40 +1200
7, 19 -- Message-Id: {147FCCE6-B0D1-44F9-B0F9-BC7D8E8E8DB7-at-aurion.nl}
7, 19 -- From: Jan Leunissen {leunissen-at-aurion.nl}
7, 19 -- To: microscopy-at-microscopy.com
7, 19 -- In-Reply-To: {200906242244.n5OMiM9X020629-at-ns.microscopy.com}
7, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 19 -- Content-Transfer-Encoding: 7bit
7, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
7, 19 -- Subject: Re: [Microscopy] viaWWW: Distance between gold and antigen in Tokuyasu staining
7, 19 -- Date: Thu, 25 Jun 2009 12:12:39 +1200
7, 19 -- References: {200906242244.n5OMiM9X020629-at-ns.microscopy.com}
7, 19 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 24 Jun 2009 21:12:39 -0500
Subject: [Microscopy] Re: Sdd vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If one does not consider any cost differential if the SDD is
higher, then IMO, there are no cons for SDDs...only plusses.

There is no LN2, no bulky Dewar, higher count rate and small
detector. The smaller size reduces column vibration if the
SDD does not have fans. There are basically three types of
cooling to accommodate the Peltier cooling method. One is
ambient cooling, two is the use of fans (not good for high mag SEM)
and three, liquid cooling. Regardless, the time from turn on
to EDS analysis is very short. Plus, the detector never suffers
from icing as do Si(Li) detectors.

In the past, the SDD detector chips had the FET in the center
of the die. The current and likely future generation of
detector chips are tear drop shaped with the FET off center.
Plus, the rings and intrinsic Silicon have been vastly improved
over the years. While the detector still needs cooling, it
is now typically done using solid state Peltier modules.

I see that some makers claim that their SDD is a flat line
of resolution versus cps. Perhaps true, but I have not tested
all makers' products. Just like Si(Li), I see that resolution
changes with cps and DT and filter time. But, SDD diversions
are hugely smaller than those of Si(Li).

At present, I think the SDD detectors are very good. The problem
area is the digital pulse processor (DPP). Claims of 1M cps are
possible but at what DT? Even so, this requires large probe diameters.
I am not convinced that resolution remains the same at increasing
cps and at the same DT. This is a fascinating area for multi-core
processing to enable the handling of high cps at low DT and with
good resolution and peak deconvolution. I hope that this will lead
to new DPPs with huge improvements. The IBM Cell processor is one
option. Plurality is another. Xilinx Spartan 3 is still another.
I think that divide and conquer applies to DPP.

My conclusion is that if you have a Si(Li) and it works for you,
keep it. If you are getting a new system or upgrading, get an SDD.
If you are in a clean room, SDD is the answer. Try the maker's
SDD and focus on their software. The detectors are IMO almost the
same. The analysis software is again, IMO, different. So the
differentiation factor is no longer the detector but the EDS GUI
and baseline software. And you also have to look at support.
My experience with xxxx SDD is such that support is not an issue,
the system is very stable.

gary g.


At 09:12 AM 6/22/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
13, 20 -- From gary-at-gaugler.com Wed Jun 24 21:12:38 2009
13, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5P2CcL2020191
13, 20 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009 21:12:38 -0500
13, 20 -- Message-Id: {200906250212.n5P2CcL2020191-at-ns.microscopy.com}
13, 20 -- Received: (qmail 32554 invoked from network); 24 Jun 2009 19:03:09 -0700
13, 20 -- Received: by simscan 1.1.0 ppid: 32546, pid: 32552, t: 0.1344s
13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
13, 20 -- by smtp2 with SMTP; 24 Jun 2009 19:03:09 -0700
13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
13, 20 -- Date: Wed, 24 Jun 2009 19:12:24 -0700
13, 20 -- To: lang-at-eps.mcgill.ca
13, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
13, 20 -- Subject: Re: [Microscopy] Sdd vs SiLi
13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
13, 20 -- In-Reply-To: {200906221612.n5MGCcvN002945-at-ns.microscopy.com}
13, 20 -- References: {200906221612.n5MGCcvN002945-at-ns.microscopy.com}
13, 20 -- Mime-Version: 1.0
13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gerd.leitinger-at-medunigraz.at
Date: Thu, 25 Jun 2009 04:26:20 -0500
Subject: [Microscopy] RE: Distance between gold and antigen in Tokuyasu staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ian White,

I have found one reference in which such distances were actually
measured: Matsubara et al. · AMPA Receptors at a Glutamatergic Synapse
J. Neurosci., July 15, 1996, 16(14):4457–4467

I think the lateral resolution of indirect immunogold is about 10-20nm.


I hope this helps

Gerd Leitinger

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both i.white-at-ucl.ac.uk as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: i.white-at-ucl.ac.uk
Name: Ian White

Organization: MRC Unit at University College London

Title-Subject: [Filtered] Distance between gold and antigen in
Tokuyasu staining

Question: If I label an antigen on a section using the Tokuyasu
technique with a primary IgG, and then a bridging IgG before finally
labelling this with protein A gold, what in approximate theoretical
terms is the maximum distance my gold partical can be from the
antigen it is "labelling"?

In other words, how long is IgG+IgG+proteinA?

Thanks for any help

Login Host: 128.40.81.24
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Jun 24 17:43:29 2009
8, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov
[130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n5OMhNwJ019936
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2009
17:43:25 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6685c793d60-at-[206.69.208.22]}
8, 11 -- Date: Wed, 24 Jun 2009 17:43:22 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: i.white-at-ucl.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Distance between gold and antigen in Tokuyasu
staining
8, 11 -- Content-Type: text/plain; charset="us-ascii" ;
format="flowed"
==============================End of -


--
Dr. Gerd Leitinger

Laboratory Coordinator
Core Facility Ultrastructure Analysis
Center for Medical Research (ZMF)
Medical University of Graz

Postal address:
Institute of Cell Biology, Histology and Embryology
Harrachgasse 21
8010 Graz
Austria
Tel. +43 316 380 4237
Fax. +43 316 380 9625
Mailto: Gerd.Leitinger-at-medunigraz.at


==============================Original Headers==============================
18, 16 -- From gerd.leitinger-at-medunigraz.at Thu Jun 25 04:26:20 2009
18, 16 -- Received: from si062.medunigraz.at (si062.meduni-graz.at [193.170.105.62])
18, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5P9QIm3029104
18, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 04:26:19 -0500
18, 16 -- Received: from connect_62-MTA by si062.medunigraz.at
18, 16 -- with Novell_GroupWise; Thu, 25 Jun 2009 11:26:18 +0200
18, 16 -- Message-Id: {4A435D53.E434.00B0.0-at-medunigraz.at}
18, 16 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
18, 16 -- Date: Thu, 25 Jun 2009 11:25:59 +0200
18, 16 -- From: "Gerd Leitinger" {gerd.leitinger-at-medunigraz.at}
18, 16 -- To: {Microscopy-at-microscopy.com}
18, 16 -- Subject: RE: Distance between gold and antigen in Tokuyasu staining
18, 16 -- Mime-Version: 1.0
18, 16 -- Content-Type: text/plain; charset=UTF-8
18, 16 -- Content-Transfer-Encoding: 8bit
18, 16 -- Content-Disposition: inline
==============================End of - Headers==============================




From: Frank.Eggert-at-ametek.de
Date: Thu, 25 Jun 2009 07:30:19 -0500
Subject: [Microscopy] viaWWW: Re: viaWWW: SDD vs. SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ian

I would have thought that the addition of Protein A gold to a
secondary and primary IgG would only have reduced the resolution
further. Surely it would be better to either attach the PAG to the
primary IgG or else use a gold labelled secondary IgG.

I understand that there may be particular reasons for doing it the way
you suggest but it will reduce the resolution of the labelling. I'm
not sufficiently experienced in these matters but I would have
thought that there would be a possibility of the PAG labelling either
the primary or secondary IgG anyway.

Good luck

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: gerd.leitinger-at-medunigraz.at

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Frank.Eggert-at-ametek.de as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: Frank.Eggert-at-ametek.de
Name: Frank Eggert

Organization: EDAX / Ametek

Title-Subject: [Filtered] Re: viaWWW: SDD vs. SiLi

Question:
Toni,

your statement: "SiLi detectors offer no advantage at all." is not
really true.

I go with you about the other properties. But there are advantages
also at Si(Li) side with the thickness of active detector area. The
SDD is no more than 0.5 mm thick. That means that the efficiency is
greatly reduced with X-rays with higher than 10 keV energy. Also,
high energy photons are able to hit the back of the detector. This
brings effects similar the usual incomplete charge collection at
X-ray entrance front. Some traces in the spectrum could come from
excitation of backside parts. The Si(Li) is 3 or 5 mm thick. This
could be important for acquisition of high-energy X-rays (15 keV and
more) like with TEM or X-ray fluorescence (which is also possible
inside a SEM). Both come with a much better sensitivity for trace
elements an therefore more sensibility with the disadvantages.

Regards

Frank

Login Host: 84.189.141.172
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 07:30:19 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PCUJAT001003
10, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 07:30:19 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c6691e41a925-at-[206.69.208.22]}
10, 11 -- Date: Thu, 25 Jun 2009 07:30:18 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: Frank.Eggert-at-ametek.de (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Re: viaWWW: SDD vs. SiLi
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 25 Jun 2009 07:52:23 -0500
Subject: [Microscopy] Re: viaWWW: SDD vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Frank Eggert is correct about the high energy advantages of Si(Li)
vs SDD. If you wish to see a plot of the relative detector
efficienies look at Figure 1 of the following article.

Detector Solid Angle Formulas for Use in X-Ray Energy
Dispersive Spectrometry, N.J. Zaluzec
Microsc. Microanal. 15, 93-98, 2009

At x-ray energies of 20 keV, the efficiency difference
as you can see in this figure is ~ 95% (3 mm SiLi) vs ~30% (0.35 mm SDD).
The thickness values of SDD range from 0.35 to 1.0 mm, with most in
at the low end of this range.

I have both SiLi and SDD systems on both TEMs and SEMs, they all
operate well, needless to say it is important to remember the
relative merits of
both types of detectors and the instrument your using.

One other point which hasn't yet been mentioned is the potential
solid angle achievable with the SDD can be significantly better
than the SiLi because of the ability to create new geometries.
There is a short article on this topic for those who might
be interested in the upcoming July issue of Microscopy-Today.

One needs to weigh all factors when choozing detectors,
and the application will dictate which is best for your applications.

Nestor
Your Friendly Neighborhood SysOp & Closet Microanalyst.

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 07:52:23 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PCqMeO015932
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 07:52:23 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240802c6691ef1d262-at-[206.69.208.22]}
8, 11 -- Date: Thu, 25 Jun 2009 07:52:22 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
8, 11 -- Subject: Re: viaWWW: SDD vs SiLi
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank.Eggert-at-ametek.de
Date: Thu, 25 Jun 2009 07:53:16 -0500
Subject: [Microscopy] viaWWW: Re: viaWWW: SDD vs. SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Frank.Eggert-at-ametek.de as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: Frank.Eggert-at-ametek.de
Name: Frank Eggert

Organization: EDAX / Ametek

Title-Subject: [Filtered] Re: viaWWW: SDD vs. SiLi

Question: Frank
What is your opinion as to the potential application of SDD on a 100
to 300kV STEM? Oak Ridge and Dow recently installed them onto their
TEM/STEM. Do we have to worry about hard x-ray deterioration? Do we
gain any counts because of the lower dead count? Will we see more of
these installed or is it just a fad?

Thanks,
Steve

Steve Bradley

Hi Steve,
I'm not really a TEM expert. Regard the basics, the count rates gain
compensates quite well the losses in efficiency. Always it depends
from which X-ray lines are to measure. Let's look to the next
experiences there about detector back side artefacts in spectra. But
anyway, it's not a fad. It may depend from application.

Frank


Login Host: 84.189.141.172
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 07:53:16 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PCrFAa017864
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 07:53:16 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c66923a4ec5d-at-[206.69.208.22]}
11, 11 -- Date: Thu, 25 Jun 2009 07:53:15 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: Frank.Eggert-at-ametek.de (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Re: viaWWW: SDD vs. SiLi
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 25 Jun 2009 08:21:36 -0500
Subject: [Microscopy] SDD on TEM/STEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve/Frank

I have operated a windowless 50 mm^2 SDD on my 300 kV FEG AAEM
since 2003 (see Microscopy & Microanalysis 2004 for details).

The detector (which was a prototype and I believe was the first SDD
on a TEM/STEM column was custom built for me by Photon Imaging
now SIIUSA) was intentionally irradiated by 300 kV backscattered electrons
for 1 hour. I attempted this experiment with the precise intent of trying to
test the damage limits of the detector and was achieved by turning off
the Objective lens. Under these conditions the 300 kV electron signal
completely swamped the detector and spectroscopy was not possible.

However, once normal conditions were restored (after about 10 minutes of
recovery time) the detector fully recovered and it returned to it's
pre-irradiation performance. (see Figure 6 in article by
Barken etal in Microscopy-Today Vol 12, #6 Nov. 2004).

I have not repeated this experiment with any of the newer crop of detectors,
or with detectors from other vendors.

Disclaimer: I have no financial interest in SIIUSA, however, I have published
collaborative research with that company.


Nestor
Your Friendly Neighborhood SysOp & Closet Microanalyst.







==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 08:21:35 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PDLYug012533
14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 08:21:34 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240804c669241d08bd-at-[206.69.208.22]}
14, 11 -- Date: Thu, 25 Jun 2009 08:21:34 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
14, 11 -- Subject: SDD on TEM/STEM's
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank.Eggert-at-ametek.de
Date: Thu, 25 Jun 2009 08:39:03 -0500
Subject: [Microscopy] Re: viaWWW: SDD vs. SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Frank.Eggert-at-ametek.de as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: Frank.Eggert-at-ametek.de
Name: Frank Eggert

Organization: EDAX / Ametek

Title-Subject: [Filtered] Re: viaWWW: SDD vs. SiLi

Question: Nestor,

thank you for the information. But my worry was not so much with the
demage of SDD, but more with artefacts inside the spectra comming
from high energy photon detector backside irradiation (scattered,
reflections, fluorescence and other artefacts) which could influence
qualitative and quanitative results, particulary in analysis of trace
elements.

The radiation demage problem was an issue, but solved some years ago.

Frank

Login Host: 84.189.141.172
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 08:39:03 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PDd3IY027786
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 08:39:03 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240809c6692e657196-at-[206.69.208.22]}
9, 11 -- Date: Thu, 25 Jun 2009 08:39:02 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: Frank.Eggert-at-ametek.de (by way of MicroscopyListserver)
9, 11 -- Subject: Re: viaWWW: SDD vs. SiLi
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 25 Jun 2009 09:10:23 -0500
Subject: [Microscopy] CPD and charging mystery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Apparently the problem with specimens charging when dried in one
critical point dryer but not in a different, but identical, one has
resolved itself spontaneously. (I described this weirdness in a recent
post to the list.) The client is now getting super-looking specimens
with this machine.

They're thinking it was residual oils from manufacturing in the CPD that
finally were removed after doing a run with a Kimwipe in the chamber. I
have my doubts, since these machines are tested before being sent out,
and I would think that many runs with ethanol would pretty much have
flushed out any oils long ago.

I suspect it may have been a bad tank of CO2 (same supplier we use) and
after changing tanks it resolved itself. But we may never know.

Thanks for all the suggestions. And sorry, Tina, trip's off. Anybody
in the market for a used rubber chicken?

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 27 -- From TindallR-at-missouri.edu Thu Jun 25 09:10:23 2009
10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PEANNP013522
10, 27 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 09:10:23 -0500
10, 27 -- X-IronPort-Anti-Spam-Filtered: true
10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAI8iQ0rRauUo/2dsb2JhbADBXwEJhzWIToJYgTUF
10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 25 Jun 2009 09:10:20 -0500
10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 27 -- Thu, 25 Jun 2009 09:10:16 -0500
10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 27 -- Content-class: urn:content-classes:message
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain;
10, 27 -- charset="us-ascii"
10, 27 -- Subject: CPD and charging mystery
10, 27 -- Date: Thu, 25 Jun 2009 09:10:15 -0500
10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD81EE-at-UM-XMAIL08.um.umsystem.edu}
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- Thread-Topic: CPD and charging mystery
10, 27 -- Thread-Index: Acn1nqmFHLR/d6CsSyuWZxYgJRYTbQ==
10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 27 -- To: {microscopy-at-microscopy.com}
10, 27 -- X-OriginalArrivalTime: 25 Jun 2009 14:10:16.0076 (UTC) FILETIME=[A9E4ECC0:01C9F59E]
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PEANNP013522
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Thu, 25 Jun 2009 09:20:24 -0500
Subject: [Microscopy] Re: viaWWW: Distance between gold and antigen in Tokuyasu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ian, Jan, Gerd, and those whose comments I've not seen.

I've made my own gold probe. I've conjugated it to antibody both
directly and through Protein A. Truth of the matter, I would never do
that again. Good quality premade probes of many sizes are ready
available commercially, and for a cost far below that which we would
realistically have if we were to do it ourselves.


If I were really interested in the probe to antigen distance and had
lots of antibody I would buy a premade probe and conjugate the antibody
directly to it. Not hard, but takes a day or two. And that would
eliminate the whole issue of the extra layers between the probe and
antigen. There are other circumstances where I would do that. Not
many, but there are others.


At the same time, if I wanted to do an indirect immuno assay, which is
all the Tokuyasu method is, then I would not waste my time with Protein
A. There are too many other things that could get involved in terms of
non-specific binding which could cause failure of the process. I would
just buy a gold:species specific AntiIgX via whomever is your favourite
supplier - they are pretty well all quite reputable ( X= IgG, IgA, IgM,
specific fragments such as fAb, etc. Take your pick.) . There are
fewer steps, and if the experiment does not give you the results you
were hoping for, or there is evidence that it didn't work, then you know
that it's probably not the gold probe that was the problem. Eliminating
that from the mix when trouble shooting is a bonus.


Just my 2 bits worth (2¢ after inflation).


Paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
13, 21 -- From paul_hazelton-at-umanitoba.ca Thu Jun 25 09:20:24 2009
13, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PEKO8Z028696
13, 21 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 09:20:24 -0500
13, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
13, 21 -- (authenticated bits=0)
13, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n5PEKNTP019654;
13, 21 -- Thu, 25 Jun 2009 09:20:23 -0500 (CDT)
13, 21 -- Message-ID: {4A4387A1.5080809-at-umanitoba.ca}
13, 21 -- Date: Thu, 25 Jun 2009 09:20:17 -0500
13, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
13, 21 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
13, 21 -- MIME-Version: 1.0
13, 21 -- To: i.white-at-ucl.ac.uk, Microscopy Listserver {microscopy-at-microscopy.com}
13, 21 -- Subject: Re: [Microscopy] viaWWW: Distance between gold and antigen in Tokuyasu
13, 21 -- staining
13, 21 -- References: {200906242247.n5OMlIQh028285-at-ns.microscopy.com}
13, 21 -- In-Reply-To: {200906242247.n5OMlIQh028285-at-ns.microscopy.com}
13, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
13, 21 -- Content-Transfer-Encoding: 8bit
13, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: lovett-at-tcnj.edu
Date: Thu, 25 Jun 2009 09:26:57 -0500
Subject: [Microscopy] stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers:

A colleague in biomedical engineering wants to examine biofouling
(bacteria and algae) on the acrylic lining of a bioreactor with the
SEM. He wants to take samples of the acrylic lining of the tank
(acrylite GP) and look for adhering cells. Does anyone have experience
with whether or not this material is stable under the electron beam? Is
there anything I can do (in addition to low acc voltage) that I can do
to minimize the chance of column contamination? (We are using a Hitachi
S-510).

Thanks,

Don (a mere biologist)

--

Donald L. Lovett E-mail: lovett-at-tcnj.edu
Professor Phone: 609-771-2876
Department of Biology Fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


==============================Original Headers==============================
7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009
7, 27 -- Received: from mailgate-out.TCNJ.EDU (mailgate-out.TCNJ.EDU [159.91.14.88])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PEQuBT009510
7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 09:26:56 -0500
7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
7, 27 -- by mailgate-out.TCNJ.EDU (Postfix) with ESMTP id 0DB78CC3BE;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU
7, 27 -- Received: from zcs.tcnj.edu ([127.0.0.1])
7, 27 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024)
7, 27 -- with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU [159.91.99.16])
7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu}
7, 27 -- Date: Thu, 25 Jun 2009 10:26:51 -0400
7, 27 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu}
7, 27 -- Organization: Department of Biology, The College of New Jersey
7, 27 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
7, 27 -- MIME-Version: 1.0
7, 27 -- To: Microscopy-at-microscopy.com
7, 27 -- Subject: stability of acrylic under the electron beam
7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 27 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 25 Jun 2009 10:13:11 -0500
Subject: [Microscopy] RE: stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
} Sent: Thursday, June 25, 2009 9:28 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] stability of acrylic under the electron beam
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Dear listers:
}
} A colleague in biomedical engineering wants to examine
} biofouling (bacteria and algae) on the acrylic lining of a
} bioreactor with the SEM. He wants to take samples of the
} acrylic lining of the tank (acrylite GP) and look for
} adhering cells. Does anyone have experience with whether or
} not this material is stable under the electron beam? Is
} there anything I can do (in addition to low acc voltage) that
} I can do to minimize the chance of column contamination? (We
} are using a Hitachi S-510).
}
} Thanks,
}
} Don (a mere biologist)
}
} --
}
} Donald L. Lovett E-mail: lovett-at-tcnj.edu
} Professor Phone: 609-771-2876
} Department of Biology Fax: 609-637-5118
} The College of New Jersey
} P.O. Box 7718
} Ewing, NJ 08628-0718
}
}
} ==============================Original
} Headers==============================
} 7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009 7, 27
} -- Received: from mailgate-out.TCNJ.EDU
} (mailgate-out.TCNJ.EDU [159.91.14.88])
} 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n5PEQuBT009510
} 7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun
} 2009 09:26:56 -0500
} 7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
} 7, 27 -- by mailgate-out.TCNJ.EDU (Postfix) with ESMTP
} id 0DB78CC3BE;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 7, 27
} -- Received: from zcs.tcnj.edu ([127.0.0.1])
} 7, 27 -- by localhost (zcs.tcnj.edu [127.0.0.1])
} (amavisd-new, port 10024)
} 7, 27 -- with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009
} 10:26:52 -0400 (EDT)
} 7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU
} [159.91.99.16])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu} 7, 27 --
} Date: Thu, 25 Jun 2009 10:26:51 -0400 7, 27 -- From: "Donald
} L. Lovett" {lovett-at-tcnj.edu} 7, 27 -- Organization:
} Department of Biology, The College of New Jersey 7, 27 --
} User-Agent: Thunderbird 2.0.0.22 (Windows/20090605) 7, 27 --
} MIME-Version: 1.0 7, 27 -- To: Microscopy-at-microscopy.com 7,
} 27 -- Subject: stability of acrylic under the electron beam
} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1;
} format=flowed 7, 27 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Thu Jun 25 10:13:10 2009
7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PFDApG027303
7, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 10:13:10 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Thu, 25 Jun 2009 10:13:10 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="US-ASCII"
7, 25 -- Subject: RE: [Microscopy] stability of acrylic under the electron beam
7, 25 -- Date: Thu, 25 Jun 2009 10:13:09 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB87E-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200906251427.n5PERnpp011572-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] stability of acrylic under the electron beam
7, 25 -- thread-index: Acn1oR+5Hp/o9mzvRnucATjzKulsvQABkkCQ
7, 25 -- References: {200906251427.n5PERnpp011572-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {lovett-at-tcnj.edu} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 25 Jun 2009 15:13:10.0047 (UTC) FILETIME=[735B32F0:01C9F5A7]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PFDApG027303
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 25 Jun 2009 11:00:37 -0500
Subject: [Microscopy] stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don,
Along with the low kV, you want a small beam current, small probe size or
high condenser lens current (3 ways of saying the same thing). This will
give you a noisy image, but you can compensate with a longer record scan
(although this will deposit more energy per unit area per unit time, ie
generate more heat).

Also keep your magnification as low as possible. This distributes the
energy over a larger area. Also, focus and stigmate near your area of
interest then shift your image to the area of interest, quickly make any
fine focus adjustments and immediately collect your image.

Practice with a piece of the acrylic before-hand. See what kind of
conditions cause the acrylic to soften and change shape. If you take it far
enough it will probably start to bubble, but then the contamination rate
really starts to climb.

I'm assuming that you don't have a cold stage, which might help reduce the
contamination rate.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
Sent: Thursday, June 25, 2009 10:30 AM
To: kenconverse-at-qualityimages.biz

Dear listers:

A colleague in biomedical engineering wants to examine biofouling
(bacteria and algae) on the acrylic lining of a bioreactor with the
SEM. He wants to take samples of the acrylic lining of the tank
(acrylite GP) and look for adhering cells. Does anyone have experience
with whether or not this material is stable under the electron beam? Is
there anything I can do (in addition to low acc voltage) that I can do
to minimize the chance of column contamination? (We are using a Hitachi
S-510).

Thanks,

Don (a mere biologist)

--

Donald L. Lovett E-mail: lovett-at-tcnj.edu
Professor Phone: 609-771-2876
Department of Biology Fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


==============================Original Headers==============================
7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009
7, 27 -- Received: from mailgate-out.TCNJ.EDU (mailgate-out.TCNJ.EDU
[159.91.14.88])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5PEQuBT009510
7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 09:26:56
-0500
7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
7, 27 -- by mailgate-out.TCNJ.EDU (Postfix) with ESMTP id 0DB78CC3BE;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU
7, 27 -- Received: from zcs.tcnj.edu ([127.0.0.1])
7, 27 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port
10024)
7, 27 -- with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009 10:26:52 -0400
(EDT)
7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU [159.91.99.16])
7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu}
7, 27 -- Date: Thu, 25 Jun 2009 10:26:51 -0400
7, 27 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu}
7, 27 -- Organization: Department of Biology, The College of New Jersey
7, 27 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
7, 27 -- MIME-Version: 1.0
7, 27 -- To: Microscopy-at-microscopy.com
7, 27 -- Subject: stability of acrylic under the electron beam
7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 27 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




==============================Original Headers==============================
22, 25 -- From kenconverse-at-qualityimages.biz Thu Jun 25 11:00:36 2009
22, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PG0Zwu011341
22, 25 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 11:00:36 -0500
22, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
22, 25 -- with ESMTP
22, 25 -- id {20090625160035312.IJZO24524-at-cdptpa-omta02.mail.rr.com} ;
22, 25 -- Thu, 25 Jun 2009 16:00:35 +0000
22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
22, 25 -- To: {lovett-at-tcnj.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
22, 25 -- Subject: RE: [Microscopy] stability of acrylic under the electron beam
22, 25 -- Date: Thu, 25 Jun 2009 12:00:25 -0400
22, 25 -- Message-ID: {4DAFE31B9072445790D40E3AC2947640-at-Ken}
22, 25 -- MIME-Version: 1.0
22, 25 -- Content-Type: text/plain;
22, 25 -- charset="us-ascii"
22, 25 -- X-Priority: 3 (Normal)
22, 25 -- X-MSMail-Priority: Normal
22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
22, 25 -- Importance: Normal
22, 25 -- thread-index: Acn1oV8N+rn0gfrDRZ2AWw4yZH17nwACuQ2w
22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
22, 25 -- In-Reply-To: {200906251429.n5PETbmm015136-at-ns.microscopy.com}
22, 25 -- Content-Transfer-Encoding: 8bit
22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PG0Zwu011341
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 25 Jun 2009 11:32:28 -0500
Subject: [Microscopy] stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe longer scan time = greater damage. Better to integrate several
fast scans to improve signal/noise ratio.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Don,
} Along with the low kV, you want a small beam current, small
} probe size or high condenser lens current (3 ways of saying
} the same thing). This will give you a noisy image, but you
} can compensate with a longer record scan (although this will
} deposit more energy per unit area per unit time, ie generate
} more heat).
}
} Also keep your magnification as low as possible. This
} distributes the energy over a larger area. Also, focus and
} stigmate near your area of interest then shift your image to
} the area of interest, quickly make any fine focus adjustments
} and immediately collect your image.
}
} Practice with a piece of the acrylic before-hand. See what
} kind of conditions cause the acrylic to soften and change
} shape. If you take it far enough it will probably start to
} bubble, but then the contamination rate really starts to climb.
}
} I'm assuming that you don't have a cold stage, which might
} help reduce the contamination rate.
}
} Ken Converse
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
} Sent: Thursday, June 25, 2009 10:30 AM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] stability of acrylic under the electron beam
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Dear listers:
}
} A colleague in biomedical engineering wants to examine biofouling
} (bacteria and algae) on the acrylic lining of a bioreactor with the
} SEM. He wants to take samples of the acrylic lining of the tank
} (acrylite GP) and look for adhering cells. Does anyone have
} experience
} with whether or not this material is stable under the
} electron beam? Is
} there anything I can do (in addition to low acc voltage) that
} I can do
} to minimize the chance of column contamination? (We are
} using a Hitachi
} S-510).
}
} Thanks,
}
} Don (a mere biologist)
}
} --
}
} Donald L. Lovett E-mail: lovett-at-tcnj.edu
} Professor Phone: 609-771-2876
} Department of Biology Fax: 609-637-5118
} The College of New Jersey
} P.O. Box 7718
} Ewing, NJ 08628-0718
}
}
} ==============================Original
} Headers==============================
} 7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009
} 7, 27 -- Received: from mailgate-out.TCNJ.EDU (mailgate-out.TCNJ.EDU
} [159.91.14.88])
} 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id
} n5PEQuBT009510
} 7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun
} 2009 09:26:56
} -0500
} 7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
} 7, 27 -- by mailgate-out.TCNJ.EDU (Postfix) with ESMTP
} id 0DB78CC3BE;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU
} 7, 27 -- Received: from zcs.tcnj.edu ([127.0.0.1])
} 7, 27 -- by localhost (zcs.tcnj.edu [127.0.0.1])
} (amavisd-new, port
} 10024)
} 7, 27 -- with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009
} 10:26:52 -0400
} (EDT)
} 7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU
} [159.91.99.16])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu}
} 7, 27 -- Date: Thu, 25 Jun 2009 10:26:51 -0400
} 7, 27 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu}
} 7, 27 -- Organization: Department of Biology, The College of
} New Jersey
} 7, 27 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
} 7, 27 -- MIME-Version: 1.0
} 7, 27 -- To: Microscopy-at-microscopy.com
} 7, 27 -- Subject: stability of acrylic under the electron beam
} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 27 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}
}
}
}
} ==============================Original
} Headers==============================
} 22, 25 -- From kenconverse-at-qualityimages.biz Thu Jun 25 11:00:36 2009
} 22, 25 -- Received: from cdptpa-omtalb.mail.rr.com
} (cdptpa-omtalb.mail.rr.com [75.180.132.123])
} 22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n5PG0Zwu011341
} 22, 25 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun
} 2009 11:00:36 -0500
} 22, 25 -- Received: from Ken ([72.227.111.133]) by
} cdptpa-omta02.mail.rr.com
} 22, 25 -- with ESMTP
} 22, 25 -- id
} {20090625160035312.IJZO24524-at-cdptpa-omta02.mail.rr.com} ;
} 22, 25 -- Thu, 25 Jun 2009 16:00:35 +0000
} 22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} 22, 25 -- To: {lovett-at-tcnj.edu} , "MSA Listserver"
} {microscopy-at-microscopy.com}
} 22, 25 -- Subject: RE: [Microscopy] stability of acrylic
} under the electron beam
} 22, 25 -- Date: Thu, 25 Jun 2009 12:00:25 -0400
} 22, 25 -- Message-ID: {4DAFE31B9072445790D40E3AC2947640-at-Ken}
} 22, 25 -- MIME-Version: 1.0
} 22, 25 -- Content-Type: text/plain;
} 22, 25 -- charset="us-ascii"
} 22, 25 -- X-Priority: 3 (Normal)
} 22, 25 -- X-MSMail-Priority: Normal
} 22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
} 22, 25 -- Importance: Normal
} 22, 25 -- thread-index: Acn1oV8N+rn0gfrDRZ2AWw4yZH17nwACuQ2w
} 22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 22, 25 -- In-Reply-To: {200906251429.n5PETbmm015136-at-ns.microscopy.com}
} 22, 25 -- Content-Transfer-Encoding: 8bit
} 22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit
} by ns.microscopy.com id n5PG0Zwu011341
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
6, 25 -- From DusevichV-at-umkc.edu Thu Jun 25 11:32:27 2009
6, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp1.exchange.umkc.edu [134.193.143.155])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PGWRLn027521
6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 11:32:27 -0500
6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Thu, 25 Jun 2009 11:32:23 -0500
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="US-ASCII"
6, 25 -- Subject: RE: [Microscopy] RE: stability of acrylic under the electron beam
6, 25 -- Date: Thu, 25 Jun 2009 11:32:22 -0500
6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB87F-at-KC-MSX1.kc.umkc.edu}
6, 25 -- In-Reply-To: {200906251601.n5PG1Ac2012202-at-ns.microscopy.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: [Microscopy] RE: stability of acrylic under the electron beam
6, 25 -- thread-index: Acn1rikkL3sH4J42Q/u7OzslNVqYOAABEGuQ
6, 25 -- References: {200906251601.n5PG1Ac2012202-at-ns.microscopy.com}
6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 25 Jun 2009 16:32:23.0225 (UTC) FILETIME=[84789A90:01C9F5B2]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PGWRLn027521
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Thu, 25 Jun 2009 11:33:18 -0500
Subject: [Microscopy] Sdd vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

gary-at-gaugler.com wrote:

} At present, I think the SDD detectors are very good. The problem
} area is the digital pulse processor (DPP). Claims of 1M cps are
} possible but at what DT? Even so, this requires large probe diameters.
} I am not convinced that resolution remains the same at increasing
} cps and at the same DT. This is a fascinating area for multi-core
} processing to enable the handling of high cps at low DT and with
} good resolution and peak deconvolution.

As someone who designs the things for a living, I feel qualified
to address this... :)

You are correct that the DPP is a major concern, but the main
issue is pile-up rejection rather than raw computing horsepower,
particularly for SEM detectors with light-element windows. I
gave a poster last year at M&M discussing this in detail, and
will be glad to provide a PDF on request.

In a nutshell, there are subtleties to the SDD which make life
difficult for pile-up detection. The main issue is that the
preamp rise time varies depending on where the X ray strikes
the sensor; the rise time is longer for longer drift times.
That limits the effectiveness of the classic technique for
low-energy pile-up detection, which is pulse width testing.
If the pulse width varies for a single X ray, the limit must
be long enough to allow for the worst-case legitimate rise time.
Otherwise, you reject single events which happen to have rise
times longer than average.

I gave a poster three weeks ago at the Munich detector conference
discussing digital methods which are not sensitive to rise time.
It is now available on the web:

http://www.hll.mpg.de/02_news/Conference/SDStalks-posters.php

(in author order, second from bottom at the moment)

On the bright side, SDDs are inherently better than Si(Li)'s on
pile-up at extremely low energies because the shaping times are
so much shorter. For a good description of the pile-up performance
on Si(Li)'s, and for software modeling/stripping of sum peaks,
see:

P. J. Statham, Microchim. Acta 155, (2006) 289-294.

He notes a resolving time for carbon of 54 uS! For an SDD, that
number should be about 2-3 uS (I haven't measured it) -- not because
the fast pile-up channels detect it, but because the pulse width
required for carbon detection is so short that you will get
separate pulses from the main edge detection channel which
triggers energy measurement.

Sum peaks become comparable in magnitude to trace-element peaks
at 50-100kcps input rate, and their relative magnitude grows
linearly with input rate. So while SDDs are indeed capable of
extreme rates, pile-up with light elements becomes important
at rates which are now easily reachable.

Rick Mott, PulseTor LLC

(disclaimer: PulseTor sells SDD upgrades and DPPs, so I have
an obvious commercial interest in the discussion)

==============================Original Headers==============================
14, 18 -- From rmott-at-pulsetor.com Thu Jun 25 11:33:18 2009
14, 18 -- Received: from smtpauth04.prod.mesa1.secureserver.net (smtpauth04.prod.mesa1.secureserver.net [64.202.165.95])
14, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5PGXHpo028806
14, 18 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 11:33:17 -0500
14, 18 -- Received: (qmail 802 invoked from network); 25 Jun 2009 16:33:15 -0000
14, 18 -- Received: from unknown (98.221.245.7)
14, 18 -- by smtpauth04.prod.mesa1.secureserver.net (64.202.165.95) with ESMTP; 25 Jun 2009 16:33:13 -0000
14, 18 -- Message-ID: {4A43A6FD.8030302-at-pulsetor.com}
14, 18 -- Date: Thu, 25 Jun 2009 12:34:05 -0400
14, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
14, 18 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
14, 18 -- MIME-Version: 1.0
14, 18 -- To: microscopy-at-microscopy.com
14, 18 -- Subject: Re: [Microscopy] Re: Sdd vs SiLi
14, 18 -- References: {200906250220.n5P2KCxK031547-at-ns.microscopy.com}
14, 18 -- In-Reply-To: {200906250220.n5P2KCxK031547-at-ns.microscopy.com}
14, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
14, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 25 Jun 2009 12:14:10 -0500
Subject: [Microscopy] stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vladimir,
You're right and most digital image systems let you choose between point,
line and frame averaging, the first giving you the quickest collection at a
given resolution and number of samples/point, while the last gives the
longest collection times, the lowest energy input, the least specimen
charging and requires the most system stability. Line averaging tends to be
a compromise on all counts.

So, yes, frame averaging should give the best results in this case.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu]
Sent: Thursday, June 25, 2009 12:35 PM
To: kenconverse-at-qualityimages.biz

I believe longer scan time = greater damage. Better to integrate several
fast scans to improve signal/noise ratio.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Don,
} Along with the low kV, you want a small beam current, small
} probe size or high condenser lens current (3 ways of saying
} the same thing). This will give you a noisy image, but you
} can compensate with a longer record scan (although this will
} deposit more energy per unit area per unit time, ie generate
} more heat).
}
} Also keep your magnification as low as possible. This
} distributes the energy over a larger area. Also, focus and
} stigmate near your area of interest then shift your image to
} the area of interest, quickly make any fine focus adjustments
} and immediately collect your image.
}
} Practice with a piece of the acrylic before-hand. See what
} kind of conditions cause the acrylic to soften and change
} shape. If you take it far enough it will probably start to
} bubble, but then the contamination rate really starts to climb.
}
} I'm assuming that you don't have a cold stage, which might
} help reduce the contamination rate.
}
} Ken Converse
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
} Sent: Thursday, June 25, 2009 10:30 AM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] stability of acrylic under the electron beam
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Dear listers:
}
} A colleague in biomedical engineering wants to examine biofouling
} (bacteria and algae) on the acrylic lining of a bioreactor with the
} SEM. He wants to take samples of the acrylic lining of the tank
} (acrylite GP) and look for adhering cells. Does anyone have
} experience
} with whether or not this material is stable under the
} electron beam? Is
} there anything I can do (in addition to low acc voltage) that
} I can do
} to minimize the chance of column contamination? (We are
} using a Hitachi
} S-510).
}
} Thanks,
}
} Don (a mere biologist)
}
} --
}
} Donald L. Lovett E-mail: lovett-at-tcnj.edu
} Professor Phone: 609-771-2876
} Department of Biology Fax: 609-637-5118
} The College of New Jersey
} P.O. Box 7718
} Ewing, NJ 08628-0718
}
}
} ==============================Original
} Headers==============================
} 7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009
} 7, 27 -- Received: from mailgate-out.TCNJ.EDU (mailgate-out.TCNJ.EDU
} [159.91.14.88])
} 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id
} n5PEQuBT009510
} 7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun
} 2009 09:26:56
} -0500
} 7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
} 7, 27 -- by mailgate-out.TCNJ.EDU (Postfix) with ESMTP
} id 0DB78CC3BE;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU
} 7, 27 -- Received: from zcs.tcnj.edu ([127.0.0.1])
} 7, 27 -- by localhost (zcs.tcnj.edu [127.0.0.1])
} (amavisd-new, port
} 10024)
} 7, 27 -- with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009
} 10:26:52 -0400
} (EDT)
} 7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU
} [159.91.99.16])
} 7, 27 -- by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
} 7, 27 -- Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
} 7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu}
} 7, 27 -- Date: Thu, 25 Jun 2009 10:26:51 -0400
} 7, 27 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu}
} 7, 27 -- Organization: Department of Biology, The College of
} New Jersey
} 7, 27 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
} 7, 27 -- MIME-Version: 1.0
} 7, 27 -- To: Microscopy-at-microscopy.com
} 7, 27 -- Subject: stability of acrylic under the electron beam
} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 27 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}
}
}
}
} ==============================Original
} Headers==============================
} 22, 25 -- From kenconverse-at-qualityimages.biz Thu Jun 25 11:00:36 2009
} 22, 25 -- Received: from cdptpa-omtalb.mail.rr.com
} (cdptpa-omtalb.mail.rr.com [75.180.132.123])
} 22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n5PG0Zwu011341
} 22, 25 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun
} 2009 11:00:36 -0500
} 22, 25 -- Received: from Ken ([72.227.111.133]) by
} cdptpa-omta02.mail.rr.com
} 22, 25 -- with ESMTP
} 22, 25 -- id
} {20090625160035312.IJZO24524-at-cdptpa-omta02.mail.rr.com} ;
} 22, 25 -- Thu, 25 Jun 2009 16:00:35 +0000
} 22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} 22, 25 -- To: {lovett-at-tcnj.edu} , "MSA Listserver"
} {microscopy-at-microscopy.com}
} 22, 25 -- Subject: RE: [Microscopy] stability of acrylic
} under the electron beam
} 22, 25 -- Date: Thu, 25 Jun 2009 12:00:25 -0400
} 22, 25 -- Message-ID: {4DAFE31B9072445790D40E3AC2947640-at-Ken}
} 22, 25 -- MIME-Version: 1.0
} 22, 25 -- Content-Type: text/plain;
} 22, 25 -- charset="us-ascii"
} 22, 25 -- X-Priority: 3 (Normal)
} 22, 25 -- X-MSMail-Priority: Normal
} 22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
} 22, 25 -- Importance: Normal
} 22, 25 -- thread-index: Acn1oV8N+rn0gfrDRZ2AWw4yZH17nwACuQ2w
} 22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 22, 25 -- In-Reply-To: {200906251429.n5PETbmm015136-at-ns.microscopy.com}
} 22, 25 -- Content-Transfer-Encoding: 8bit
} 22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit
} by ns.microscopy.com id n5PG0Zwu011341
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
6, 25 -- From DusevichV-at-umkc.edu Thu Jun 25 11:32:27 2009
6, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp1.exchange.umkc.edu
[134.193.143.155])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n5PGWRLn027521
6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 11:32:27
-0500
6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by
KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Thu, 25 Jun 2009 11:32:23 -0500
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="US-ASCII"
6, 25 -- Subject: RE: [Microscopy] RE: stability of acrylic under the
electron beam
6, 25 -- Date: Thu, 25 Jun 2009 11:32:22 -0500
6, 25 -- Message-ID:
{032EC4F75A527A4FA58C5B1B5DECFBB3062CB87F-at-KC-MSX1.kc.umkc.edu}
6, 25 -- In-Reply-To: {200906251601.n5PG1Ac2012202-at-ns.microscopy.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: [Microscopy] RE: stability of acrylic under the
electron beam
6, 25 -- thread-index: Acn1rikkL3sH4J42Q/u7OzslNVqYOAABEGuQ
6, 25 -- References: {200906251601.n5PG1Ac2012202-at-ns.microscopy.com}
6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 25 Jun 2009 16:32:23.0225 (UTC)
FILETIME=[84789A90:01C9F5B2]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n5PGWRLn027521
==============================End of - Headers==============================




==============================Original Headers==============================
19, 25 -- From kenconverse-at-qualityimages.biz Thu Jun 25 12:14:10 2009
19, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PHE9OG026018
19, 25 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 12:14:09 -0500
19, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
19, 25 -- with ESMTP
19, 25 -- id {20090625171408480.IWSK19511-at-cdptpa-omta03.mail.rr.com} ;
19, 25 -- Thu, 25 Jun 2009 17:14:08 +0000
19, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
19, 25 -- To: {DusevichV-at-umkc.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
19, 25 -- Subject: RE: [Microscopy] stability of acrylic under the electron beam
19, 25 -- Date: Thu, 25 Jun 2009 13:13:58 -0400
19, 25 -- Message-ID: {B0C088687370478E813B7921D72F82EB-at-Ken}
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Type: text/plain;
19, 25 -- charset="us-ascii"
19, 25 -- X-Priority: 3 (Normal)
19, 25 -- X-MSMail-Priority: Normal
19, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
19, 25 -- Importance: Normal
19, 25 -- thread-index: Acn1svdfqHI7JsOWS/yM/3K8hiyMUgABJQsQ
19, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 25 -- In-Reply-To: {200906251635.n5PGZMk6002901-at-ns.microscopy.com}
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PHE9OG026018
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 25 Jun 2009 12:32:09 -0500
Subject: [Microscopy] Re: CPD and charging mystery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rubber chicken! I love rubber chickens. It reminds me of mom's home cooking.

Have a great weekend everybody.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.


--- On Thu, 6/25/09, TindallR-at-missouri.edu {TindallR-at-missouri.edu} wrote:

} From: TindallR-at-missouri.edu {TindallR-at-missouri.edu}
} Subject: [Microscopy] CPD and charging mystery
} To: vapatpxs-at-yahoo.com
} Date: Thursday, June 25, 2009, 2:15 PM
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} Apparently the problem with specimens charging when dried
} in one
} critical point dryer but not in a different, but identical,
} one has
} resolved itself spontaneously.  (I described this
} weirdness in a recent
} post to the list.)  The client is now getting
} super-looking specimens
} with this machine.
}
} They're thinking it was residual oils from manufacturing in
} the CPD that
} finally were removed after doing a run with a Kimwipe in
} the chamber.  I
} have my doubts, since these machines are tested before
} being sent out,
} and I would think that many runs with ethanol would pretty
} much have
} flushed out any oils long ago. 
}
} I suspect it may have been a bad tank of CO2 (same supplier
} we use) and
} after changing tanks it resolved itself.  But we may
} never know.
}
} Thanks for all the suggestions.  And sorry, Tina,
} trip's off.  Anybody
} in the market for a used rubber chicken?
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
} Sons of Norway:  http://www.sofn.com
}
}
}
}
} ==============================Original
} Headers==============================
} 10, 27 -- From TindallR-at-missouri.edu
} Thu Jun 25 09:10:23 2009
} 10, 27 -- Received: from
} mxtip01-umsystem-out.um.umsystem.edu
} (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
} 10, 27 --     by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n5PEANNP013522
} 10, 27 --     for {microscopy-at-microscopy.com} ;
} Thu, 25 Jun 2009 09:10:23 -0500
} 10, 27 -- X-IronPort-Anti-Spam-Filtered: true
} 10, 27 -- X-IronPort-Anti-Spam-Result:
} ApoEAI8iQ0rRauUo/2dsb2JhbADBXwEJhzWIToJYgTUF
} 10, 27 -- Received: from unknown (HELO
} um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
} 10, 27 --   by
} mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 25 Jun 2009
} 09:10:20 -0500
} 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu
} ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with
} Microsoft SMTPSVC(6.0.3790.3959);
} 10, 27 --      Thu, 25 Jun 2009
} 09:10:16 -0500
} 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 10, 27 -- Content-class: urn:content-classes:message
} 10, 27 -- MIME-Version: 1.0
} 10, 27 -- Content-Type: text/plain;
} 10, 27 --     charset="us-ascii"
} 10, 27 -- Subject: CPD and charging mystery
} 10, 27 -- Date: Thu, 25 Jun 2009 09:10:15 -0500
} 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD81EE-at-UM-XMAIL08.um.umsystem.edu}
} 10, 27 -- X-MS-Has-Attach:
} 10, 27 -- X-MS-TNEF-Correlator:
} 10, 27 -- Thread-Topic: CPD and charging mystery
} 10, 27 -- Thread-Index: Acn1nqmFHLR/d6CsSyuWZxYgJRYTbQ==
} 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 10, 27 -- To: {microscopy-at-microscopy.com}
} 10, 27 -- X-OriginalArrivalTime: 25 Jun 2009 14:10:16.0076
} (UTC) FILETIME=[A9E4ECC0:01C9F59E]
} 10, 27 -- Content-Transfer-Encoding: 8bit
} 10, 27 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n5PEANNP013522
} ==============================End of -
} Headers==============================
}





==============================Original Headers==============================
12, 22 -- From vapatpxs-at-yahoo.com Thu Jun 25 12:32:09 2009
12, 22 -- Received: from web46101.mail.sp1.yahoo.com (web46101.mail.sp1.yahoo.com [68.180.199.118])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5PHW97R008913
12, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 25 Jun 2009 12:32:09 -0500
12, 22 -- Received: (qmail 61415 invoked by uid 60001); 25 Jun 2009 17:32:08 -0000
12, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1245951128; bh=9Wg8wolSb25i0oZk0cHOBOZK1Oa9vRXz+ifhndJVK7c=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=AIy1K1ZtsICIjHwgDyrnco+WiCpyISfhQH4sESwXpkCOHn0svzl6k6whSLHrIAtuAz7aL7LS9rW+PaFXEcR85K1FsWPA1gT9kgRFl4eQmIkaMtbesylkwb5m2SNmCeTVwaZp2/U7wDtDWjfArkpSuTixB74D/wOf/Zcnsw1663g=
12, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 22 -- s=s1024; d=yahoo.com;
12, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
12, 22 -- b=uHTDHhaLPkJuLQlS2d+vZq85xVvEYUod7AFd8bwPICcvJrMXL3lK4VImL4145yWWKfwgfUvk8n+qgKXxTcHZwlF6LNmQN0JlNdGMv6sSakvM3PqhbIEqBK3lATHt3DKPIhELB9oG+Og85R4VFpG8QRRf8IVHmL2vjSVcQasW108=;
12, 22 -- Message-ID: {783165.53514.qm-at-web46101.mail.sp1.yahoo.com}
12, 22 -- X-YMail-OSG: LK5O7lkVM1n8o25axTu8K5NWSmumKzG31S6sXjr3G_qXrMXyz6Mgdfo7yEoviBaL59o9DPXkLaBwLW674DcfwGKEmyLQpT3MMpYh0TJcUk4yog0maFlo55IZ4e95DGxz6VzESeSlOZG4E2GQDbkwflGJcJo0WzcaM6xhtUQRFKTI.caY68JelFkwGuJJOFPsqIu.h25vr2ReFkJZTp3q3rn7djZWxStT2LnTrTMgxBMh_50I5T7C6ST3bOVD__2mOE6zW1IfBUA2FOUq4UXk7LEtjYXe8AyhZ72Tkdis3R_bLraiCGbZt0.SIPAkQ18X98ykvPzOLhBOXynwcf6d6S4CuEhyEuk7LXKz_jONjO4-
12, 22 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Thu, 25 Jun 2009 10:32:08 PDT
12, 22 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
12, 22 -- Date: Thu, 25 Jun 2009 10:32:08 -0700 (PDT)
12, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 22 -- Subject: Re: [Microscopy] CPD and charging mystery
12, 22 -- To: TindallR-at-missouri.edu, MSA BB {Microscopy-at-Microscopy.Com}
12, 22 -- MIME-Version: 1.0
12, 22 -- Content-Type: text/plain; charset=iso-8859-1
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5PHW97R008913
==============================End of - Headers==============================




From: kmiller-at-admin.nmt.edu
Date: Thu, 25 Jun 2009 17:50:54 -0500
Subject: [Microscopy] viaWWW: Service Source Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kmiller-at-admin.nmt.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kmiller-at-admin.nmt.edu
Name: Kimela Miller

Organization: New Mexico Tech (NMT)

Title-Subject: [Filtered] Service Source

Question: NMT has acquired a FEI/Philips CM-30 TEM and need to have
it de-installed, moved, and installed. I am looking for
sources who can provide this service. I will be issuing
a bid. Thanks - K
kmiller-at-admin.nmt.edu or 575.835.5881

Login Host: 129.138.18.112
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jun 25 17:50:54 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PMoqOB011824
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 17:50:54 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c669afb2c00f-at-[206.69.208.22]}
6, 11 -- Date: Thu, 25 Jun 2009 17:50:51 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: kmiller-at-admin.nmt.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Service Source Needed
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 25 Jun 2009 23:11:39 -0500
Subject: [Microscopy] Re: Sdd vs SiLi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the feedback. Wow!! The conference looks
like it was awesome. I would liked to attended that
conference if I knew about it.

First off, can you put the DPP units for Si(Li) in
perspective to SDD DPP units? Not a challenge, just
a question. We agree, I think, that SDD processing
is more difficult than Si(Li).

How much of the DPP can be accomplished via Spartan 3
or Virtex 2? Where is it's distinguishing points for an
ASIC as referred to in the conference notes? Why an FPGA
vs an ASIC? Please reinforce my/your basis for this option.

Like I said, I do not see a flat line of cps versus DT and
resolution. If not, why not?

I think that SDD is now the norm and Si(Li)is history.

Ask me for details.

gary g.





At 09:34 AM 6/25/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
17, 20 -- From gary-at-gaugler.com Thu Jun 25 23:11:38 2009
17, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5Q4BaWB017535
17, 20 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 23:11:37 -0500
17, 20 -- Message-Id: {200906260411.n5Q4BaWB017535-at-ns.microscopy.com}
17, 20 -- Received: (qmail 7862 invoked from network); 25 Jun 2009 21:31:13 -0700
17, 20 -- Received: by simscan 1.1.0 ppid: 7859, pid: 7860, t: 0.1467s
17, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
17, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
17, 20 -- by smtp1 with SMTP; 25 Jun 2009 21:31:13 -0700
17, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
17, 20 -- Date: Thu, 25 Jun 2009 21:11:28 -0700
17, 20 -- To: rmott-at-pulsetor.com
17, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
17, 20 -- Subject: Re: [Microscopy] Sdd vs SiLi
17, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
17, 20 -- In-Reply-To: {200906251634.n5PGYcEW000482-at-ns.microscopy.com}
17, 20 -- References: {200906251634.n5PGYcEW000482-at-ns.microscopy.com}
17, 20 -- Mime-Version: 1.0
17, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Fri, 26 Jun 2009 02:19:49 -0500
Subject: [Microscopy] stability of acrylic under the electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Don!

As you apparently only need the morphological information, I would definitely use a nice coating.
Not only does it discharge your sample and disperse the beam energy but it also increases the contrast.
I would still use fast scanning (with averaging as suggested) and small probe sizes to avoid accumulation of energy locally.
Bacteria and algae, we are talking µm here! In all cases you will definitely won't need high mag.
I don't think that the challenge is in the examination. I would be more concerned about the preparation.

Best regards,

Stephane

 


----- Original Message ----
X-from: "lovett-at-tcnj.edu" {lovett-at-tcnj.edu}
To: nizets2-at-yahoo.com
Sent: Thursday, June 25, 2009 4:31:08 PM

Dear listers:

A colleague in biomedical engineering wants to examine biofouling
(bacteria and algae) on the acrylic lining of a bioreactor with the
SEM.  He wants to take samples of the acrylic lining of the tank
(acrylite GP) and look for adhering cells.  Does anyone have experience
with whether or not this material is stable under the electron beam?  Is
there anything I can do (in addition to low acc voltage) that I can do
to minimize the chance of column contamination?  (We are using a Hitachi
S-510).

Thanks,

Don (a mere biologist)

--

Donald L. Lovett                E-mail:  lovett-at-tcnj.edu
Professor                    Phone:  609-771-2876
Department of Biology                  Fax:  609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ  08628-0718


==============================Original Headers==============================
7, 27 -- From lovett-at-tcnj.edu Thu Jun 25 09:26:56 2009
7, 27 -- Received: from mailgate-out.TCNJ.EDU (mailgate-out.TCNJ.EDU [159.91.14.88])
7, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5PEQuBT009510
7, 27 --     for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2009 09:26:56 -0500
7, 27 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209])
7, 27 --     by mailgate-out.TCNJ.EDU (Postfix) with ESMTP id 0DB78CC3BE;
7, 27 --     Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 27 --     by zcs.tcnj.edu (Postfix) with ESMTP id A783F33756A;
7, 27 --     Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU
7, 27 -- Received: from zcs.tcnj.edu ([127.0.0.1])
7, 27 --     by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024)
7, 27 --     with ESMTP id OEkq3fEbViNM; Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Received: from [159.91.99.16] (TCNJ-99-16.TCNJ.EDU [159.91.99.16])
7, 27 --     by zcs.tcnj.edu (Postfix) with ESMTP id 5E74D337502;
7, 27 --     Thu, 25 Jun 2009 10:26:52 -0400 (EDT)
7, 27 -- Message-ID: {4A43892B.5090305-at-tcnj.edu}
7, 27 -- Date: Thu, 25 Jun 2009 10:26:51 -0400
7, 27 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu}
7, 27 -- Organization: Department of Biology, The College of New Jersey
7, 27 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
7, 27 -- MIME-Version: 1.0
7, 27 -- To: Microscopy-at-microscopy.com
7, 27 -- Subject: stability of acrylic under the electron beam
7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 27 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================






==============================Original Headers==============================
24, 25 -- From nizets2-at-yahoo.com Fri Jun 26 02:19:49 2009
24, 25 -- Received: from web110809.mail.gq1.yahoo.com (web110809.mail.gq1.yahoo.com [67.195.13.232])
24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5Q7JmIg021025
24, 25 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2009 02:19:48 -0500
24, 25 -- Received: (qmail 70208 invoked by uid 60001); 26 Jun 2009 07:19:48 -0000
24, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1246000788; bh=WIkh7ul7dyHVFcSBcCvRD+YEqDYaX/8uA7xYoI3D0no=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=3q3zkXNMmIFcTPcObwygSFdT340VcpnS9HKsZ/pJkdDhHjcobL12oOIrysXNqwC66cRc1buRbFThIKfAD5i3rORzxW2DDT6mEyUiwdjhrbW4mhAHFhh74NKla5Xo0TMqR5HxRYzoxybHORqQugmgKdKxX/LYnSeac1rwslq64Nk=
24, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
24, 25 -- s=s1024; d=yahoo.com;
24, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
24, 25 -- b=y1wACDcCQWCEfJAPJ4qtRvhlN0a6/X55+/SswCaEDMjhNkf6FKCtnczifdwmvS+cVhpuZxrwJQQ05E08qcbOtT7jULrU4TsaWsP/ihNBKtkEYNKvV+yg69YPfR6WvS3RYMzpX708we33e2V5PoJLVNKoSMbrkgQeJ7Tx0vrTqYU=;
24, 25 -- Message-ID: {235682.69395.qm-at-web110809.mail.gq1.yahoo.com}
24, 25 -- X-YMail-OSG: JytqEesVM1lYVqUOxkPqb_kku4QEOW3uI..3z9SkfMPTcJcLhA.3klzTPFWDQLS3LjgVCa0Q_pYslmikHVdLPiPmQQ4ZRjV_9jXWRSnrEQpway_UBt_QEUcti8y5SNLhqJ3uPFyRRjBCNxmBiRQlMHCFClYiT_TeHQocjAhuueK3o3U.ot8lWuo7k4b2k2D6Czoej6AhLZ50mi2lo904a_7wtyniPeb0_CoDxIcQEHFlP2BYoKVltnwcIsUtcDLrHAu7ad3TKX0Tyrzr1vwKT58fABUWxhtu1ckMj8EyKmuy0y_VHll1iI73sd0RWhCAJMpOjQVXTC79o_JPYZiM.RmQnzkNKotvaWx5xxNZQCU-
24, 25 -- Received: from [80.122.101.100] by web110809.mail.gq1.yahoo.com via HTTP; Fri, 26 Jun 2009 00:19:47 PDT
24, 25 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
24, 25 -- References: {200906251431.n5PEV8eY017655-at-ns.microscopy.com}
24, 25 -- Date: Fri, 26 Jun 2009 00:19:47 -0700 (PDT)
24, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
24, 25 -- Subject: Re: [Microscopy] stability of acrylic under the electron beam
24, 25 -- To: lovett-at-tcnj.edu
24, 25 -- Cc: microscopy-at-microscopy.com
24, 25 -- In-Reply-To: {200906251431.n5PEV8eY017655-at-ns.microscopy.com}
24, 25 -- MIME-Version: 1.0
24, 25 -- Content-Type: text/plain; charset=iso-8859-1
24, 25 -- Content-Transfer-Encoding: 8bit
24, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5Q7JmIg021025
==============================End of - Headers==============================




From: frank.eggert-at-ametek.de
Date: Fri, 26 Jun 2009 08:15:14 -0500
Subject: [Microscopy] viaWWW: SDD pile-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both frank.eggert-at-ametek.de as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: frank.eggert-at-ametek.de
Name: Frank Eggert

Organization: EDAX / Ametek

Title-Subject: [Filtered] SDD pile-up

Question: Before:
I do have financial interest in this stuff, because I'm working for EDAX.

The sumary:
Digital Pulse Processor (DPP) + Pile-Up correction software should be
both a requirement to handle SDD spectrometers reasonable. Data
acquisition with 10 kcps and much more without any worry about limits
and the requirements above is producing dirty spectra.

Details:
The SDD is an dramtic improvement in energy dispersive X-ray
spectrometry, for sure. But sometimes the things were exaggerated
regarding the specs and properties. High count rates statements are
one of the most seductive things. It's really true, there is an
principle advantage in relation to Si(Li) to achieve better count
rate with same other properties of the spectrum. Despite of there is
no question that any SDD is able to perform count rates much higher
than the Si(Li) ever or with much better resolution at medium count
rates (because shorter shaping times), this is not the full truth.

There are no principle advantages regarding the pile-up effect of low
energy photons (with ultra thin detector windows). Sure, because of
shorter basis shaping time of SDD there is also an advantage, but
only with a factor of about 3 or so. That means, a 2000 cps of an
Si(Li) is quite similar to a spectrum from SDD with about 6000 cps.
All higher count rates produce spectra which are dirty in comparison
to a Si(Li), which is not able to run higher with best resolution.
The reason is a principle thing: You can only get best energy
resolution or best time resolution. Therefore all tries to improve
the time resolution with shorter shaping times (hidden processing
channels for pile-up rejection in digital or analogue pulse
processors) comes with poorer energy resolution (not the resolution
in visible spectrum!). That's why this is always a compromise of
both, because we need some energy resolution to distinguish still
soft X-rays from electronical and detector noise. Therefore all
pile-up rejection approaches in pulse processors have to fight with
this, to recognize low energy photons also in time-resolving channel.
Therefore always the energy treshold in pile-up rejection channels
are higher than with the visual spectrum, in principle. This problem
is not with 1...2 keV and higher. The time resolution can be set very
short with enough remaining energy resolution to trigger photons } 1
keV. But we are discussing about SEM spectrometers with ultra thin
windows and ability to start with 0.1 keV energies.

Therefore, an additional spectra processing pile-up correction
software is necessarily needed to process the spectra of SDD in high
count rate cases (} 10 kcps). This is always necessary. No pulse
processor is able to do this job completely. Only then it is possible
to run the count rate higher with keeping the spectra clean.

To Gery:
It is true, the performance of DPP pile-up rejection depends from
pulse-pair resolving time. You describe in your poster some live
pattern recognition in the DPP which may push the limits towards
better low energy counts pile-up rejection. But also with your last
two graphs in your poster, the pulse-pair resolving of O+O is more
than 0.2 us and of O with other peaks between 0.15 and 0.2 us. It is
much better only if no low-energy photons are inside the game (Fe+Fe
with 0.05 us). This demonstrates again the principle problem with
low-energy pile up rejection. Unfortunately I could not found any
high cps spectrum example with demonstration of the improvements
inside your poster. Therefore I have simulated the situation for 50
kcps and have still found clear sum peaks Al+O with 0.2us and Al+Al
with 0.05us, possibly less than usual but still present. It becomes
more complicated with C (below 500 eV, Oxygen is fortunately still
barely below problem-boundary). I miss a statement regard { 500eV!
But Carbon and all the L- and M-lines are also often inside the
spectra.

Finally, also considering your contribution I would state again:
There are issues with low energy photons and high cps. The best way
to handle indeed is using an digital pulse processor with some clever
processing possibilities. Rising DPP processing speeds will shift the
limits in future. But a post processing with pile-up correction is
also needed (considering the DPP parameters) to get clean spectra. If
this is not available you should keep the cps below 10kcps also with
SDD and not like advertised with 100kcps or even more. If you have no
low-energy counts inside the spectrum, then don't worry. But this is
rather rare. An alternative is putting a Be-window onto the detector
with loosing light-elements detectability.

The Si(Li) was never faced to these problems, simply because it was
not able to run with 50 kcps and more (and if, then without Carbon
detection also in spectrometric channel).

Frank




Login Host: 84.189.143.37
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Fri Jun 26 08:15:14 2009
17, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5QDFD6C022397
17, 11 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2009 08:15:14 -0500
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240801c66a7a0a2dd1-at-[206.69.208.22]}
17, 11 -- Date: Fri, 26 Jun 2009 08:15:12 -0500
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: frank.eggert-at-ametek.de (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: SDD pile-up
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nama1213-at-hotmail.com
Date: Sat, 27 Jun 2009 07:41:13 -0500
Subject: [Microscopy] viaWWW: EDS on Leo VPFESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nama1213-at-hotmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nama1213-at-hotmail.com
Name: Naemah Al-Mansour

Organization: Kuwait University

Title-Subject: [Filtered] VPFESEM

Question: My best greetings to you all. I have a question and would
like to get your help. I have used LEO microscope (VPFESEM) with EDX
attachment to measure elemental composition of fresh plant tissues.
Lately a cryo-attachemnet of the instrument was operated and when I
tried to use EDX system (without cryo-operation), I could not get
(CPS) from the system and was unable to complete the work. Does any
one know why I am not getting cps from EDX system? I was thinking if
the anti-contaminator plate installed in the LEO microscope chamber
(when cryo-attachment is connected) might obstruct the EDX but from
my knowledge we can perform EDX under cryo conditions as well (so how
come cps was not detected in the system). Can we perform EDX analyses
if cryo-attachment was attached (but not operated)? Please help me in
this problem as I am running of time and need to complete my
microscopy work. Many thanks Naemah Al-Mansour

Login Host: 78.89.1.123
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Sat Jun 27 07:41:13 2009
6, 11 -- Received: from [10.0.1.6] ([206.69.208.18])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5RCfC0x009464
6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 27 Jun 2009 07:41:13 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c66bc3cc0474-at-[156.80.114.159]}
6, 11 -- Date: Sat, 27 Jun 2009 08:41:12 -0400
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: nama1213-at-hotmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: EDS on Leo VPFESEM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Sat, 27 Jun 2009 14:58:19 -0500
Subject: [Microscopy] Junk drawer clean-out.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just cleaned out my junk drawer, and I cam across a Channeltron
multiplier assembly, what looks like a photomultiplier, model 4770E,
serial number 45804.

Does anybody have use for this? If so, contact me off-list and it's yours.

--Justin.

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
4, 31 -- From kraftpiano-at-gmail.com Sat Jun 27 14:58:19 2009
4, 31 -- Received: from mail-bw0-f208.google.com (mail-bw0-f208.google.com [209.85.218.208])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5RJwIYY005324
4, 31 -- for {microscopy-at-microscopy.com} ; Sat, 27 Jun 2009 14:58:18 -0500
4, 31 -- Received: by bwz4 with SMTP id 4so2952281bwz.18
4, 31 -- for {microscopy-at-microscopy.com} ; Sat, 27 Jun 2009 12:58:18 -0700 (PDT)
4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
4, 31 -- :from:to:content-type:content-transfer-encoding;
4, 31 -- bh=vy9qWp3HSXKKRKivINtMGSSx9kHmmlPCU6RtEJpOE4s=;
4, 31 -- b=PfTog7YhFJXGM6Nqu/+HDJseGt0vBhtc7OKl6+ErXjAZZgKfmLhAG3XtyB1eMGG76v
4, 31 -- PQUgHJ+8rFh7g9bKK9SBFr/01xkiGs0aOEjHMUgJoFJ9M/PTwKAIJz4n8erml+E9Bzra
4, 31 -- fSGt119CsjfvhHzFz6WkILM4Dh5gtzPzc+LFk=
4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 31 -- d=gmail.com; s=gamma;
4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
4, 31 -- :content-transfer-encoding;
4, 31 -- b=POD0EsAv/rHzwIje8fa7efNrlrlNDZ0GQvoCvTAs8kfF4mUP9FOE/617EaLQOXy4Bk
4, 31 -- wX7tlHEjxLg7TLRo1Sccf1PI9fvV9rmdecTfXqVHKl/I9xON5825YYLsiX4HtA4Lz17X
4, 31 -- R0o0GQj6kiY622MNIgIfG6y/xqhNtxHix3zLI=
4, 31 -- MIME-Version: 1.0
4, 31 -- Received: by 10.204.51.210 with SMTP id e18mr5177567bkg.38.1246132697985; Sat,
4, 31 -- 27 Jun 2009 12:58:17 -0700 (PDT)
4, 31 -- Date: Sat, 27 Jun 2009 15:58:17 -0400
4, 31 -- Message-ID: {25e2b0d20906271258n101eb117kda581d55a1b9a326-at-mail.gmail.com}
4, 31 -- Subject: Junk drawer clean-out.
4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
4, 31 -- To: microscopy-at-microscopy.com
4, 31 -- Content-Type: text/plain; charset=ISO-8859-1
4, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Sun, 28 Jun 2009 08:33:28 -0500
Subject: [Microscopy] Re: viaWWW: SDD pile-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

frank.eggert-at-ametek.de wrote:

} Therefore, an additional spectra processing pile-up correction
} software is necessarily needed to process the spectra of SDD in high
} count rate cases (} 10 kcps). This is always necessary. No pulse
} processor is able to do this job completely. Only then it is possible
} to run the count rate higher with keeping the spectra clean.
}
(and a bit later...)
} Therefore I have simulated the situation for 50
} kcps and have still found clear sum peaks Al+O with 0.2us and Al+Al
} with 0.05us, possibly less than usual but still present. It becomes
} more complicated with C (below 500 eV, Oxygen is fortunately still
} barely below problem-boundary). I miss a statement regard { 500eV!
} But Carbon and all the L- and M-lines are also often inside the
} spectra.

Sorry, I got busy and it took a couple of days to get back to this.

I agree entirely with your post. My point is that while residual
sum peaks will always occur at high rates, the performance
of the real-time pulse-to-pulse processing in the DPP affects the
ease and accuracy of software pile-up correction later.

If the DPP is set up properly, the remaining sum peaks should be:
(a) right at the expected sum energy of the parent lines, and
(b) have good Gaussian shapes and predictable resolution.

This isn't always true. I've seen sum peaks which are low in
energy by 50+ eV and have significant low-side tails. The DPP
design choices which result in that are detailed in the poster.

Also, even if the software model for the sum peak location and
shape is perfect (which it generally isn't; all the ones I've
seen so far leave some residual artifacts at sufficiently high
rates), the size of the sum peak being stripped away
affects the minimum detectable limit (MDL) of an underlying
trace peak. It's no different from conventional peak
deconvolution; the larger the overlapping peak, the more its
statistical variance contributes to the error in the smaller
underlying peak.

So the DPP and the software are complementary. If the DPP
gives smaller sum peaks with good shape, it makes the software's
job easier and results in smaller residual errors after stripping.

Rick Mott, PulseTor LLC

==============================Original Headers==============================
9, 18 -- From rmott-at-pulsetor.com Sun Jun 28 08:33:28 2009
9, 18 -- Received: from smtpauth14.prod.mesa1.secureserver.net (smtpauth14.prod.mesa1.secureserver.net [64.202.165.39])
9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5SDXREL011933
9, 18 -- for {microscopy-at-microscopy.com} ; Sun, 28 Jun 2009 08:33:28 -0500
9, 18 -- Received: (qmail 14038 invoked from network); 28 Jun 2009 13:33:27 -0000
9, 18 -- Received: from unknown (98.221.245.7)
9, 18 -- by smtpauth14.prod.mesa1.secureserver.net (64.202.165.39) with ESMTP; 28 Jun 2009 13:33:26 -0000
9, 18 -- Message-ID: {4A477169.7070007-at-pulsetor.com}
9, 18 -- Date: Sun, 28 Jun 2009 09:34:33 -0400
9, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
9, 18 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
9, 18 -- MIME-Version: 1.0
9, 18 -- To: microscopy-at-microscopy.com
9, 18 -- Subject: Re: [Microscopy] viaWWW: SDD pile-up
9, 18 -- References: {200906261322.n5QDMmA4000756-at-ns.microscopy.com}
9, 18 -- In-Reply-To: {200906261322.n5QDMmA4000756-at-ns.microscopy.com}
9, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Mon, 29 Jun 2009 03:02:27 -0500
Subject: [Microscopy] SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

First thanks to Frank, Rick and other for there very usefull comments
about SDD.

As soon said by others, the high count rate capability is the first
caracteristic of the SDD which is shown off. Now, what about sensitivity ?

We work with a cold FEG-SEM, and have needs for EDS on thin films on
bulk surfaces or sub micron and nano particules.
We need always to adapte the primary energy to the thickness which is to
be analysed, with the compromise to get some signal on the right peaks,
all in the 400-4000 eV range (O-K, L line of transition metals, Al-K,
Si-K, M lines of heavy metals, etc). This impose energies in the 2-7
keV. In these conditions, the max beam current is in the 50-150 pA
range, and I'm glad when I get 500 c/s in the best conditions ! By the
way, our SEM cannot give more then 2 nA, what gives in best cases 12-15
kcps at } 20 keV on a 30mm2 SiLi, and 10µs time constant...
So I've no interest in the tenth kcps capabilities of a SDD.

In such a situation, what would be the pro and cont of SDD versus SiLi.
Is there an noticeable difference in sensitivity (counts/nA/mm2) between
both ? Apart to get free of the N2l duty, what would be the gains with
a SDD ?

Thanks

--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


==============================Original Headers==============================
9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Jun 29 03:02:27 2009
9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156])
9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5T82QKX022279
9, 29 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 03:02:26 -0500
9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n5T82Pf9088497
9, 29 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 10:02:25 +0200 (CEST)
9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 29 -- (No client certificate requested)
9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 499C110002C2
9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 29 Jun 2009 09:54:20 +0200 (CEST)
9, 29 -- Message-ID: {4A487503.1020804-at-ipcms.u-strasbg.fr}
9, 29 -- Date: Mon, 29 Jun 2009 10:02:11 +0200
9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 29 -- User-Agent: Thunderbird 2.0.0.22 (X11/20090608)
9, 29 -- MIME-Version: 1.0
9, 29 -- To: Microscopy-at-microscopy.com
9, 29 -- Subject: SiLi v. SDD other quest.
9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 29 -- Content-Transfer-Encoding: 8bit
9, 29 -- X-IPCMS-MailScanner: Found to be clean
9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.156]); Mon, 29 Jun 2009 10:02:25 +0200 (CEST)
9, 29 -- X-Virus-Scanned: ClamAV 0.94.2/9517/Mon Jun 29 01:08:06 2009 on mr6.u-strasbg.fr
9, 29 -- X-Virus-Status: Clean
9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
9, 29 -- autolearn=disabled version=3.2.5
9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr6.u-strasbg.fr
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Mon, 29 Jun 2009 06:34:57 -0500
Subject: [Microscopy] Re: SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

jacques.faerber-at-ipcms.u-strasbg.fr wrote:

}
} In such a situation, what would be the pro and cont of SDD versus SiLi.
} Is there an noticeable difference in sensitivity (counts/nA/mm2) between
} both ? Apart to get free of the N2l duty, what would be the gains with
} a SDD ?
}

Assuming the same geometry, there's no improvement in (counts/nA/mm2).

However, SDDs can have better ultimate resolution than Si(Li)s
of the same size. One reason is very low device capacitance which
doesn't change (much) with active area for an SDD, whereas it is
proportional to area for a Si(Li).

So you could get some benefit in peak separation and P/B ratio,
which in your situation is more significant for low energy lines.
Because electronic noise adds in quadrature to the energy-dependent
component of resolution, a given resolution advantage at Mn-K
becomes a larger difference as a fraction of peak width for low
energies.

Rick Mott, PulseTor LLC

==============================Original Headers==============================
6, 18 -- From rmott-at-pulsetor.com Mon Jun 29 06:34:57 2009
6, 18 -- Received: from smtpauth05.prod.mesa1.secureserver.net (smtpauth05.prod.mesa1.secureserver.net [64.202.165.99])
6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n5TBYuDo016565
6, 18 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 06:34:56 -0500
6, 18 -- Received: (qmail 30256 invoked from network); 29 Jun 2009 11:34:55 -0000
6, 18 -- Received: from unknown (98.221.245.7)
6, 18 -- by smtpauth05.prod.mesa1.secureserver.net (64.202.165.99) with ESMTP; 29 Jun 2009 11:34:55 -0000
6, 18 -- Message-ID: {4A48A726.8080103-at-pulsetor.com}
6, 18 -- Date: Mon, 29 Jun 2009 07:36:06 -0400
6, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
6, 18 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
6, 18 -- MIME-Version: 1.0
6, 18 -- To: microscopy-at-microscopy.com
6, 18 -- Subject: Re: [Microscopy] SiLi v. SDD other quest.
6, 18 -- References: {200906290810.n5T8A3V1002225-at-ns.microscopy.com}
6, 18 -- In-Reply-To: {200906290810.n5T8A3V1002225-at-ns.microscopy.com}
6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: j.wittig-at-vanderbilt.edu
Date: Mon, 29 Jun 2009 07:00:07 -0500
Subject: [Microscopy] viaWWW: M and M 2009 Golf

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.wittig-at-vanderbilt.edu as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.wittig-at-vanderbilt.edu
Name: James Wittig

Organization: Vanderbilt University

Title-Subject: [Filtered] M and M 2009 Golf

Question: Although there will not be an official
2009 M and M golf tournament in Richmond, there
will still be an opportunity to golf with your
fellow microscopists in the inaugural ìSinclair
Cupî. The tournament, which is a tribute to
Robert Sinclairís MSA 2009 Distinguished
Scientist Award, will be held at the Independence
Golf Club http://independencegolfclub.com on
Sunday, July 26th. For more information, please
email Jim Wittig at j.wittig-at-vanderbilt.edu .

Login Host: 193.175.131.12
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Mon Jun 29 07:00:07 2009
7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5TC062p032148
7, 13 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 07:00:06 -0500
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240800c66e5d336fbd-at-[206.69.208.22]}
7, 13 -- Date: Mon, 29 Jun 2009 07:00:06 -0500
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: j.wittig-at-vanderbilt.edu (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: M and M 2009 Golf
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5TC062p032148
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Mon, 29 Jun 2009 08:54:11 -0500
Subject: [Microscopy] 5th Workshop on PFM and SNDM - NIMS, Japan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

You are cordially invited to attend the International Tutorial Workshop on Piezoresponse Force Microscopy - Instrumentation, Theory, and Applications (5th in series), to be held at National Institute for Materials Science (NIMS), Tsukuba, Japan on August 19-21. The workshop precedes the IMF-ISAF conference at Xi'an Jiaotong University in China.

The workshop will feature a series of tutorial and invited lectures on instrumental and theoretical aspects of PFM, nanoscale ferroelectricity, and emerging techniques such as Scanning Nonlinear Dielectric Microscopy. The tutorial speakers include Andrei Kholkin (U. Aveiro), Sergei Kalinin (ORNL), Alexei Gruver
man (UNL), Roger Proksch (Asylum), Venkat Gopalan (Penn State), James Gimjewski (UCLA) and Jiangyu Li (UW). The detailed information on the workshop is available on-line at http://www.nims.go.jp/omc/pfm5.html.

For the first time, the workshop will feature tutorials on SNDM, tunneling experiments in PFM, relaxor ferroelectric imaging and spectroscopy, novel dynamic modes and spectroscopic imaging, reflecting the tremendous progress in the field in the last year.

The workshop will also feature the series of hands-on tutorials and equipment demoes in PFM and SNDM operation.

Yours

Kenji Kitamura and Sergei V. Kalinin

Kenji KITAMURA, Dr.Sc.
Fellow (NIMS), Principal Investigator (WPI-MANA)
National Institute for Materials Science (NIMS)
WPI Center for Materials Nanoarchitechtonics(WPI-MANA)
1-1 Namiki, Tsukuba 305-0044, Japan

Affiliate Professor
University of Washington
College of Engineering
NIMS Overseas Operation Office
Roosevelt Commons Bldg #440
4311 11th Ave NE, Seattle, WA 98105, USA

Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
13, 24 -- From sergei2-at-ornl.gov Mon Jun 29 08:54:10 2009
13, 24 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110])
13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5TDsA2b021573
13, 24 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 08:54:10 -0500
13, 24 -- Received: from emroute3.ornl.gov ([127.0.0.1])
13, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
13, 24 -- with ESMTP id {0KM00062M6M6K8-at-emroute3.ornl.gov} for
13, 24 -- microscopy-at-microscopy.com; Mon, 29 Jun 2009 09:54:07 -0400 (EDT)
13, 24 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov
13, 24 -- (PMDF V6.4 #31561) id {0KM0006016M5QJ-at-emroute3.ornl.gov} ; Mon,
13, 24 -- 29 Jun 2009 09:54:05 -0400 (EDT)
13, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
13, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
13, 24 -- with ESMTP id {0KM00067H6M4HY-at-emroute3.ornl.gov} ; Mon,
13, 24 -- 29 Jun 2009 09:54:05 -0400 (EDT)
13, 24 -- Date: Mon, 29 Jun 2009 09:54:04 -0400
13, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
13, 24 -- Subject: 5th Workshop on PFM and SNDM - NIMS, Japan
13, 24 -- To: microscopy-at-microscopy.com, Kenji {zta06251-at-nifty.com}
13, 24 -- Message-id: {4A48C77C.9090800-at-ornl.gov}
13, 24 -- MIME-version: 1.0
13, 24 -- Content-type: text/plain; format=flowed; charset=windows-1252
13, 24 -- Content-transfer-encoding: 7bit
13, 24 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
==============================End of - Headers==============================




From: Frank.Eggert-at-ametek.de
Date: Mon, 29 Jun 2009 22:17:53 -0500
Subject: [Microscopy] viaWWW: SDD pile-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Frank.Eggert-at-ametek.de as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: Frank.Eggert-at-ametek.de
Name: Frank Eggert

Organization: EDAX / Ametek

Title-Subject: [Filtered] SDD pile-up

Question:
To Rick:

I agree fully with your statement:
"So the DPP and the software are complementary."

Sorry about, I've named you wrongly with 'Gery' in my last post.

Frank

Login Host: 84.189.146.111
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Jun 29 22:17:53 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5U3Hp0i016353
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jun 2009 22:17:52 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c66f3447d971-at-[206.69.208.22]}
9, 11 -- Date: Mon, 29 Jun 2009 22:17:50 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: Frank.Eggert-at-ametek.de (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: SDD pile-up
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mcgeejj-at-kapl.gov
Date: Tue, 30 Jun 2009 08:02:53 -0500
Subject: [Microscopy] FIB Microscopist job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have justed posted a position for an experienced FIB Microscopist,
preferably with materials science background, to be responsible for a
new FIB (dual SEM/Ion columns) instrument lab in early 2010. If
interested, please see the position description at the Monster site
(search under 'KAPL FIB'):

http://jobsearch.monster.com

I will be at the M&M meeting in Richmond, July 26-30, if anyone wants to
meet and discuss this position.

Thanks.

Jim

************************************
James J. McGee
Lead Engineer, Chemistry Programs
BMPC- KAPL
Mail Bin 149
PO Box 1072
Schenectady, NY 12301-1072

Tel: 518-395-4612
Fax: 518-395-4340
email: mcgeejj-at-kapl.gov
************************************


==============================Original Headers==============================
3, 26 -- From mcgeejj-at-kapl.gov Tue Jun 30 08:02:53 2009
3, 26 -- Received: from ibetpms06.bias2000.bettis.gov (bettis.gov [65.170.176.212])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5UD2rXa008447
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2009 08:02:53 -0500
3, 26 -- Received: from atlas.bias2000.bettis.gov (unverified) by ibetpms06.bias2000.bettis.gov
3, 26 -- (Clearswift SMTPRS 5.2.9) with SMTP id {T8f3ccfc751ac1002861aac-at-ibetpms06.bias2000.bettis.gov} for {microscopy-at-microscopy.com} ;
3, 26 -- Tue, 30 Jun 2009 09:02:53 -0400
3, 26 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830);
3, 26 -- Tue, 30 Jun 2009 09:02:52 -0400
3, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 26 -- Content-class: urn:content-classes:message
3, 26 -- MIME-Version: 1.0
3, 26 -- Content-Type: text/plain;
3, 26 -- charset="us-ascii"
3, 26 -- Subject: FIB Microscopist job
3, 26 -- Date: Tue, 30 Jun 2009 09:02:52 -0400
3, 26 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB8301E6C137-at-ibetpex02.bias2000.bettis.gov}
3, 26 -- X-MS-Has-Attach:
3, 26 -- X-MS-TNEF-Correlator:
3, 26 -- Thread-Topic: FIB Microscopist job
3, 26 -- Thread-Index: Acn5gxLgRj+01uG1SPelQl6lGHKWQQ==
3, 26 -- From: "McGee, James" {mcgeejj-at-kapl.gov}
3, 26 -- To: {microscopy-at-microscopy.com}
3, 26 -- X-OriginalArrivalTime: 30 Jun 2009 13:02:52.0718 (UTC) FILETIME=[13EE34E0:01C9F983]
3, 26 -- Content-Transfer-Encoding: 8bit
3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5UD2rXa008447
==============================End of - Headers==============================




From: mirandan-at-mail.nih.gov
Date: Tue, 30 Jun 2009 12:56:39 -0500
Subject: [Microscopy] EM - Senior Scientist Opportunity in Frederick, MD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SAIC-Frederick, Inc. is looking for a Senior Scientist (Requisition 149958) to manage our Electron Microscopy (EM) Laboratory at our location in Frederick, MD.

The Electron Microscopy Laboratory (EML) is part of the Advanced Technology Program (ATP) and offers ultrasctructural analysis, viral diagnosis, and immunoelectron imaging of cell and tissue samples using transmission electron microscopy (TEM) and scanning electron microscopy. The Senior Scientist will manage the staff and operations of the EML at SAIC-Frederick, Inc. Specific duties include managerial oversight of the EM laboratory's core services, interface with intra- and extramural investigators to provide EM characterization support, and methods development and research using techniques such as energy dispersive x-ray spectroscopy (EDS), cryogenics, and 3D tomography on biological samples. The Senior Scientist will also interpret of results and prepare written reports, and work with an interdisciplinary team of scientists to characterize nanomaterials intended for cancer therapeutics and diagnostics.

REQUIRED SKILLS: Possession of a doctoral degree from an accredited college/ university in a field related to Chemistry, Biology, Material Science, Engineering or Physics. Foreign educated candidates who have completed part or all of their education outside of the United States must have their foreign education evaluated by an SAIC-approved accrediting organization to assure that it has met the equivalency of the qualifications of degree work in the United States. In addition to education requirements, a minimum of five (5) years of related experience. A minimum of three (3) years using electron microscopy (TEM, SEM). Experience with energy dispersive x-ray spectroscopy (EDS), cryogenics, and 3D tomography is required. This position is subject to obtaining a Public Trust Clearance.

DESIRED SKILLS: Experience with nanomaterials and associated equipment/methods; ability to work independently in a cross-disciplinary setting; previous experience setting-up laboratories. Experience with evaluation of cancer biomarkers.

For more information and to apply, please visit our website at www.saic-frederick.com and look for Requisition 149958.


==============================Original Headers==============================
6, 31 -- From mirandan-at-mail.nih.gov Tue Jun 30 12:56:38 2009
6, 31 -- Received: from nihxway6out.hub.nih.gov (nihxway6out.hub.nih.gov [128.231.90.114])
6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5UHucUt003623
6, 31 -- for {microscopy-at-microscopy.org} ; Tue, 30 Jun 2009 12:56:38 -0500
6, 31 -- X-IronPortListener: Outbound_SMTP
6, 31 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115])
6, 31 -- by nihxway6out.hub.nih.gov with ESMTP; 30 Jun 2009 13:54:48 -0400
6, 31 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
6, 31 -- Tue, 30 Jun 2009 13:54:47 -0400
6, 31 -- Received: from NIHHTRC.nih.gov (156.40.71.74) by NIHHT01.nih.gov
6, 31 -- (156.40.71.20) with Microsoft SMTP Server (TLS) id 8.1.358.0; Tue, 30 Jun
6, 31 -- 2009 13:54:47 -0400
6, 31 -- Received: from NIHMLBX01.nih.gov ([156.40.71.31]) by NIHHTRC.nih.gov
6, 31 -- ([156.40.71.74]) with mapi; Tue, 30 Jun 2009 13:54:47 -0400
6, 31 -- From: "Miranda, Nelmarie (NIH/NCI) [C]" {mirandan-at-mail.nih.gov}
6, 31 -- To: "'microscopy-at-microscopy.org'" {microscopy-at-microscopy.org}
6, 31 -- Date: Tue, 30 Jun 2009 13:54:46 -0400
6, 31 -- Subject: EM - Senior Scientist Opportunity in Frederick, MD
6, 31 -- Thread-Topic: EM - Senior Scientist Opportunity in Frederick, MD
6, 31 -- Thread-Index: Acn5q9r2E0lLL8SzTmSq4WSfCkzBqA==
6, 31 -- Message-ID: {3D7FD38AAD34A84982D156F22703B0E096ECCB0B-at-NIHMLBX01.nih.gov}
6, 31 -- Accept-Language: en-US
6, 31 -- Content-Language: en-US
6, 31 -- X-MS-Has-Attach:
6, 31 -- X-MS-TNEF-Correlator:
6, 31 -- acceptlanguage: en-US
6, 31 -- Content-Type: text/plain; charset="us-ascii"
6, 31 -- MIME-Version: 1.0
6, 31 -- X-OriginalArrivalTime: 30 Jun 2009 17:54:47.0609 (UTC) FILETIME=[DB9E7A90:01C9F9AB]
6, 31 -- Content-Transfer-Encoding: 8bit
6, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5UHucUt003623
==============================End of - Headers==============================




From: clrodekoh-at-presby.edu
Date: Tue, 30 Jun 2009 20:34:53 -0500
Subject: [Microscopy] viaWWW: JSM 840 (Older JEOL SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both clrodekoh-at-presby.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: clrodekoh-at-presby.edu
Name: Chad Rodekohr

Organization: Presbyterian College

Title-Subject: [Filtered] JSM 840 (Older JEOL SEM)

Question: Hi everyone,

I am putting together a donated SEM (JSM 840), and I have become
aware that a crucial part is missing: the high voltage oil tank which
supplies the bias for the gun. Does anyone have any 840's that you
are cannibalizing for old parts? If so is there a high voltage oil
tank I could purchase from you? If not do you have any ideas where I
could find one?

Thanks so much!,
Chad



Login Host: 68.115.193.23
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Jun 30 20:34:52 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n611Yq3g016759
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2009 20:34:52 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c6706d9c4aef-at-[206.69.208.22]}
10, 11 -- Date: Tue, 30 Jun 2009 20:34:51 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: clrodekoh-at-presby.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: JSM 840 (Older JEOL SEM)
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 30 Jun 2009 20:49:28 -0500
Subject: [Microscopy] Re: viaWWW: JSM 840 (Older JEOL SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

why not take it apart and fix the problem??

These are not all that complicated. But I agree
that it is not totally simple.

gary g.



At 06:36 PM 6/30/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Tue Jun 30 20:49:28 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n611nSgf031395
10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2009 20:49:28 -0500
10, 20 -- Message-Id: {200907010149.n611nSgf031395-at-ns.microscopy.com}
10, 20 -- Received: (qmail 8867 invoked from network); 30 Jun 2009 18:39:31 -0700
10, 20 -- Received: by simscan 1.1.0 ppid: 8863, pid: 8864, t: 0.1043s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 30 Jun 2009 18:39:31 -0700
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Tue, 30 Jun 2009 18:49:18 -0700
10, 20 -- To: clrodekoh-at-presby.edu
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] viaWWW: JSM 840 (Older JEOL SEM)
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200907010136.n611aNVH019165-at-ns.microscopy.com}
10, 20 -- References: {200907010136.n611aNVH019165-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 1 Jul 2009 02:32:31 -0500
Subject: [Microscopy] Re: SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To Rick

Thanks for your feedback. But as my english is not so good, I'm not
shure to understand right your last sentence :
} Because electronic noise adds in quadrature to the energy-dependent
} component of resolution, a given resolution advantage at Mn-K
} becomes a larger difference as a fraction of peak width for low
} energies.
Could you explain a little bit more ?
Thanks in advance



To Jim

Thanks for your advice. I made some tests at high tilt years ago, and it
was diffcult to find a good compromise between the layer thickness, the
analysed surface and the need to image what is really analysed. It works
with tall samples which have a very homogeneous coating in the } 100 nm
range. But with tenth of nm, one need quasi grazzing tilt angles, what
makes the situation more complexe.

But right, I had a bit absolutly eliminated that way to work.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} jacques.faerber-at-ipcms.u-strasbg.fr wrote:
}
}
} } }
} } } In such a situation, what would be the pro and cont of SDD versus SiLi.
} } } Is there an noticeable difference in sensitivity (counts/nA/mm2) between
} } } both ? Apart to get free of the N2l duty, what would be the gains with
} } } a SDD ?
} } }
} }
}
} Assuming the same geometry, there's no improvement in (counts/nA/mm2).
}
} However, SDDs can have better ultimate resolution than Si(Li)s
} of the same size. One reason is very low device capacitance which
} doesn't change (much) with active area for an SDD, whereas it is
} proportional to area for a Si(Li).
}
} So you could get some benefit in peak separation and P/B ratio,
} which in your situation is more significant for low energy lines.
} Because electronic noise adds in quadrature to the energy-dependent
} component of resolution, a given resolution advantage at Mn-K
} becomes a larger difference as a fraction of peak width for low
} energies.
}
} Rick Mott, PulseTor LLC
}


Jim Quinn a écrit :
} Have you considered putting a high tilt
} on your sample and using higher energy?
}
} JQuinn
}
}
}
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi all
} }
} } First thanks to Frank, Rick and other for there very usefull comments
} } about SDD.
} }
} } As soon said by others, the high count rate capability is the first
} } caracteristic of the SDD which is shown off. Now, what about sensitivity ?
} }
} } We work with a cold FEG-SEM, and have needs for EDS on thin films on
} } bulk surfaces or sub micron and nano particules.
} } We need always to adapte the primary energy to the thickness which is to
} } be analysed, with the compromise to get some signal on the right peaks,
} } all in the 400-4000 eV range (O-K, L line of transition metals, Al-K,
} } Si-K, M lines of heavy metals, etc). This impose energies in the 2-7
} } keV. In these conditions, the max beam current is in the 50-150 pA
} } range, and I'm glad when I get 500 c/s in the best conditions ! By the
} } way, our SEM cannot give more then 2 nA, what gives in best cases 12-15
} } kcps at } 20 keV on a 30mm2 SiLi, and 10µs time constant...
} } So I've no interest in the tenth kcps capabilities of a SDD.
} }
} } In such a situation, what would be the pro and cont of SDD versus SiLi.
} } Is there an noticeable difference in sensitivity (counts/nA/mm2) between
} } both ? Apart to get free of the N2l duty, what would be the gains with
} } a SDD ?
} }
} } Thanks
} }
} } --
} } J. Faerber
} } IPCMS-GSI
} } (Institut de Physique et Chimie des Matériaux de Strasbourg
} } Groupe Surface et Interfaces)
} } 23, rue de Loess ; BP43
} } 67034 Strasbourg CEDEX 2
} } France
} }
} } Tel 00 33(0)3 88 10 71 01
} } Fax 00 33(0)3 88 10 72 48
} } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
} }
} }
}
}

==============================Original Headers==============================
13, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Jul 1 02:32:30 2009
13, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.155])
13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n617WUh3029431
13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 02:32:30 -0500
13, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
13, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n617WS2S072622
13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 09:32:29 +0200 (CEST)
13, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
13, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
13, 31 -- (No client certificate requested)
13, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id E594B10002D1
13, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Jul 2009 09:23:00 +0200 (CEST)
13, 31 -- Message-ID: {4A4B10B5.7070703-at-ipcms.u-strasbg.fr}
13, 31 -- Date: Wed, 01 Jul 2009 09:31:01 +0200
13, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
13, 31 -- User-Agent: Thunderbird 2.0.0.22 (X11/20090608)
13, 31 -- MIME-Version: 1.0
13, 31 -- To: Microscopy-at-microscopy.com
13, 31 -- Subject: Re: [Microscopy] SiLi v. SDD other quest.
13, 31 -- References: {200906291340.n5TDekm12320-at-www.matscieng.sunysb.edu}
13, 31 -- In-Reply-To: {200906291340.n5TDekm12320-at-www.matscieng.sunysb.edu}
13, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
13, 31 -- Content-Transfer-Encoding: 8bit
13, 31 -- X-IPCMS-MailScanner: Found to be clean
13, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
13, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.155]); Wed, 01 Jul 2009 09:32:29 +0200 (CEST)
13, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9531/Wed Jul 1 07:09:17 2009 on mr5.u-strasbg.fr
13, 31 -- X-Virus-Status: Clean
13, 31 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
13, 31 -- autolearn=disabled version=3.2.5
13, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr5.u-strasbg.fr
==============================End of - Headers==============================




From: yu_zongsen-at-yahoo.com
Date: Wed, 1 Jul 2009 04:57:21 -0500
Subject: [Microscopy] visualization spectrum image (EELS line-scan) as a surface plot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues


I have acquired a spectrum image (EELS line-scan across a grain boundary, about 50 measurements ), I want to visualize the EELS line-scan as a surface plot , and to see the signals change when crossing grain boundary. However, whatever I try with Digital Micrograph , a greyscale image is always attached underneath the surface plot, how can I get rid of this image ?


Thanks

Zaoli




==============================Original Headers==============================
8, 20 -- From yu_zongsen-at-yahoo.com Wed Jul 1 04:57:20 2009
8, 20 -- Received: from web31813.mail.mud.yahoo.com (web31813.mail.mud.yahoo.com [68.142.207.76])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n619vKMI016531
8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 04:57:20 -0500
8, 20 -- Received: (qmail 26037 invoked by uid 60001); 1 Jul 2009 09:57:20 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1246442240; bh=nlaU+Uw3eNaMhOJ7YgrW8O1BZnrA+ffLQj+4OjrxtkY=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=6C8uR+RInEiPz46TnpNXR5t/kO8y23GuOn+xa0PEO115XisndiHxEjr7W7o4YS0JuikzcYGf1ff1vTIbuFe5gr2WCWtfD0HdJ1s46pOA9+VhzF9E6jVE0BBgvav3e7wsacLXnysyb0uzxW1N2CBMXWBYBqZr81Jx5lqGGjYOegc=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=qrcX0Fn/+Cz0PdE7PQ7GDPSExNxi5sbhVOmnEwKNRW0FZFc/RUtSSeGfeoRiDCSM8mMrGa4Us16g4DDtLeY7fWTLwsc7oXAYuQsJnXcGsrROGT8MR4fVV158s7vEdIfu2PVjdiUZNUcube5qn6xKpvy8i4XMR/xijuX7c059wQI=;
8, 20 -- Message-ID: {301881.25777.qm-at-web31813.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG: 8k1hbU0VM1kdBr6p0Mhtre.2s44XXvNI04OXsrFqV28KDNd8W7YLa4pNq5nEEj7St1xD2WsMOph5Uu452_GxmYEFWeFaQSyswaQEgDM5lFOLx3ti3BmfBjkRBcaKU5d4DlE01apb6SMOvJ99qd60hazFj3vZsEII434lOFVqPXCloh977pps35eUljHS6qrNoJz9fQJNXoQ1qlPLSpWp5WEK5S8QZs2a_u0hGd_o1X7qjWO7CqJ5op8eqXM1fMK.syVPenggppWuElDmgh821w6sxv.BjcaV5EwwZao_NWtb9FelnlM-
8, 20 -- Received: from [193.170.20.79] by web31813.mail.mud.yahoo.com via HTTP; Wed, 01 Jul 2009 02:57:19 PDT
8, 20 -- X-Mailer: YahooMailClassic/6.0.18 YahooMailWebService/0.7.338
8, 20 -- Date: Wed, 1 Jul 2009 02:57:19 -0700 (PDT)
8, 20 -- From: zongsen yu {yu_zongsen-at-yahoo.com}
8, 20 -- Subject: visualization spectrum image (EELS line-scan) as a surface plot
8, 20 -- To: Microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 1 Jul 2009 06:58:54 -0500
Subject: [Microscopy] Re: viaWWW: JSM 840 (Older JEOL SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gee Gary,
Perhaps you could help me out, because after 32 years of repairing SEMs I
have yet to figure out how to take apart and repair something that is
missing. If I could learn how to do that I might just be able to retire!

Thanks for you're your help.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Tuesday, June 30, 2009 9:51 PM
To: kenconverse-at-qualityimages.biz

why not take it apart and fix the problem??

These are not all that complicated. But I agree
that it is not totally simple.

gary g.



At 06:36 PM 6/30/2009, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Tue Jun 30 20:49:28 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n611nSgf031395
10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2009 20:49:28
-0500
10, 20 -- Message-Id: {200907010149.n611nSgf031395-at-ns.microscopy.com}
10, 20 -- Received: (qmail 8867 invoked from network); 30 Jun 2009 18:39:31
-0700
10, 20 -- Received: by simscan 1.1.0 ppid: 8863, pid: 8864, t: 0.1043s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 30 Jun 2009 18:39:31 -0700
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Tue, 30 Jun 2009 18:49:18 -0700
10, 20 -- To: clrodekoh-at-presby.edu
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] viaWWW: JSM 840 (Older JEOL SEM)
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200907010136.n611aNVH019165-at-ns.microscopy.com}
10, 20 -- References: {200907010136.n611aNVH019165-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




==============================Original Headers==============================
23, 25 -- From kenconverse-at-qualityimages.biz Wed Jul 1 06:58:54 2009
23, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n61BwqEP002593
23, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 06:58:53 -0500
23, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
23, 25 -- with ESMTP
23, 25 -- id {20090701115848496.ELKD26680-at-cdptpa-omta04.mail.rr.com} ;
23, 25 -- Wed, 1 Jul 2009 11:58:48 +0000
23, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
23, 25 -- To: {gary-at-gaugler.com} , "MSA Listserver" {microscopy-at-microscopy.com}
23, 25 -- Subject: RE: [Microscopy] Re: viaWWW: JSM 840 (Older JEOL SEM)
23, 25 -- Date: Wed, 1 Jul 2009 07:58:39 -0400
23, 25 -- Message-ID: {DDDCF165FC8F45999E3B56388E1B1414-at-Ken}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain;
23, 25 -- charset="us-ascii"
23, 25 -- X-Priority: 3 (Normal)
23, 25 -- X-MSMail-Priority: Normal
23, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
23, 25 -- Importance: Normal
23, 25 -- Thread-Index: Acn57nIvFSsR2kVrR92c46CGkViJagAVDnUg
23, 25 -- In-Reply-To: {200907010151.n611pEvS002923-at-ns.microscopy.com}
23, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n61BwqEP002593
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Wed, 1 Jul 2009 18:22:29 -0500
Subject: [Microscopy] Magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ken

clearly, that's why you're the expert and we aren't.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
X-from: kenconverse-at-qualityimages.biz

A colleague of mine is preparing to install an FEI Quanta450-FEG. The
site survey by FEI of the room designated to accommodate the SEM
revealed that there is a horizontal magnetic field component which is
above FEI specs, all other specs for acoustic, electrical, and
electromagnetic background were met. The measurements revealed
horizontal peak-to-peak magnetic field of 4.3 mGauss whereas the specs
require 3 mGauss or less.

My question is does anyone have experience with such levels of
magnetic field strength deviations from the specs and to what extent
would this affect the maximum attainable resolution?


Krassimir N. Bozhilov

University of California
Riverside, CA 92521

tel: 951 827 2998
fax: 951 827 2489

bozhilov-at-ucr.edu



==============================Original Headers==============================
9, 19 -- From bozhilov-at-ucr.edu Wed Jul 1 18:22:29 2009
9, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.214.80])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n61NMSqh015242
9, 19 -- for {microscopy-at-microscopy.org} ; Wed, 1 Jul 2009 18:22:28 -0500
9, 19 -- Received: from [138.23.185.168] ([138.23.185.168])
9, 19 -- by sentoku.ucr.edu (MOS 3.10.5-GA)
9, 19 -- with ESMTP id FYR75031 (AUTH bozhilov-at-ucr.edu)
9, 19 -- for {microscopy-at-microscopy.org} ;
9, 19 -- Wed, 1 Jul 2009 16:22:26 -0700 (PDT)
9, 19 -- Message-Id: {00EAA329-DFA6-43E2-8A3A-A941B8F7A2A9-at-ucr.edu}
9, 19 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
9, 19 -- To: microscopy-at-microscopy.org
9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
9, 19 -- Subject: Magnetic field
9, 19 -- Date: Wed, 1 Jul 2009 16:22:26 -0700
9, 19 -- X-Mailer: Apple Mail (2.935.3)
9, 19 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================




From: avergason-at-tetracore.com
Date: Wed, 1 Jul 2009 19:02:41 -0500
Subject: [Microscopy] viaWWW: TEM Primary fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both avergason-at-tetracore.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: avergason-at-tetracore.com
Name: Amy Vergason

Organization: Tetracore, Inc

Title-Subject: [Filtered] TEM Primary fixatives

Question: Hi Everyone,

I am trying to find the recipes for TEM Primary fixatives 1, 2, and 3
which are referred to in "Current Protocols in Microbiology" and I am
having no luck. The protocol says "see recipe" but I cannot find
any. Can anyone help?

Thank you!
Amy

Login Host: 66.3.246.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Jul 1 19:02:41 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6202eQu030144
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 19:02:41 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c671a98f592e-at-[206.69.208.22]}
8, 11 -- Date: Wed, 1 Jul 2009 19:02:39 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: avergason-at-tetracore.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: TEM Primary fixatives
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: opmills-at-mtu.edu
Date: Wed, 1 Jul 2009 19:03:14 -0500
Subject: [Microscopy] viaWWW: Reichert Jung Ultra Milling machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: opmills-at-mtu.edu
Name: Owen MIlls

Organization: Michigan Tech University

Title-Subject: [Filtered] Reichert Jung Ultra Milling machine

Question: All,

We have one of these milling machines, vintage 1982. Ours was
installed and then moved to make room for other equipment. It was
not reconnected to power. I want to reconnect it for use but all the
manuals are in German. I'd like to talk to someone who has one of
these machines. Contact me offline.

Thanks,

Owen Mills

Login Host: 141.219.192.45
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Jul 1 19:03:14 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6203DwO031182
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 19:03:14 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c671a9a95f55-at-[206.69.208.22]}
9, 11 -- Date: Wed, 1 Jul 2009 19:03:13 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Reichert Jung Ultra Milling machine
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Wed, 1 Jul 2009 22:27:45 -0500
Subject: [Microscopy] Fwd: viaWWW: TEM Primary fixatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Amy,

These designations are not something commonly accepted. Do you know
the authors of the protocol? If yes, I would then look up their papers.

Generally, if you provide more detail, perhaps there will be people
who recognize a clue and can help.

Good luck,
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

Begin forwarded message:

} From: "avergason-at-tetracore.com" {avergason-at-tetracore.com}
} Date: July 1, 2009 8:03:31 PM EDT
} To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com}
} Subject: [Microscopy] viaWWW: TEM Primary fixatives
} Reply-To: "avergason-at-tetracore.com" {avergason-at-tetracore.com}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both avergason-at-tetracore.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: avergason-at-tetracore.com
} Name: Amy Vergason
}
} Organization: Tetracore, Inc
}
} Title-Subject: [Filtered] TEM Primary fixatives
}
} Question: Hi Everyone,
}
} I am trying to find the recipes for TEM Primary fixatives 1, 2, and 3
} which are referred to in "Current Protocols in Microbiology" and I am
} having no luck. The protocol says "see recipe" but I cannot find
} any. Can anyone help?
}
} Thank you!
} Amy
}
} Login Host: 66.3.246.66
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Wed Jul 1 19:02:41 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n6202eQu030144
} 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 19:02:41
} -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c671a98f592e-at-[206.69.208.22]}
} 8, 11 -- Date: Wed, 1 Jul 2009 19:02:39 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: avergason-at-tetracore.com (by way of
} MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: TEM Primary fixatives
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
8, 23 -- From vladislav_speransky-at-nih.gov Wed Jul 1 22:27:44 2009
8, 23 -- Received: from out5.smtp.messagingengine.com (out5.smtp.messagingengine.com [66.111.4.29])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n623RiL8025304
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 22:27:44 -0500
8, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id 8C599389140
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 23:27:43 -0400 (EDT)
8, 23 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
8, 23 -- by compute1.internal (MEProxy); Wed, 01 Jul 2009 23:27:43 -0400
8, 23 -- X-Sasl-enc: FG554+8Gs6jeSkJHOoVVxFIWVey6u8YhhxG2pz/WNcpi 1246505263
8, 23 -- Received: from [192.168.1.5] (pool-173-73-11-193.washdc.fios.verizon.net [173.73.11.193])
8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id 228481A4D0
8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jul 2009 23:27:43 -0400 (EDT)
8, 23 -- Message-Id: {2DFC46D2-0E18-4211-B57E-0F08A0305B99-at-nih.gov}
8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Mime-Version: 1.0 (Apple Message framework v935.3)
8, 23 -- Subject: Fwd: [Microscopy] viaWWW: TEM Primary fixatives
8, 23 -- Date: Wed, 1 Jul 2009 23:27:43 -0400
8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2B1D4920-at-NIHMLBX02.nih.gov}
8, 23 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: ALawrence-at-entomology.msstate.edu
Date: Thu, 2 Jul 2009 08:21:00 -0500
Subject: [Microscopy] M&M 2009 bursary/volunteer note

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings one and all,
Less than one month till Richmond. There are still a few areas where we could use the assistance of a student bursary or volunteer.

Student bursaries are paid $10 per hour for helping out during the meetings by serving as room monitors for paper sessions, or staffing the MegaBooth and Internet Cafe. Although volunteers are not paid hourly as the student bursaries are, they do receive some compensation. Both bursaries and volunteers get an additional $10 cash to help with meals for each morning and/or afternoon session worked.

If you would like to help as either a student bursary or volunteer, please let me know.

Thanks,
Amanda Lawrence
Electron Microscope Center
Mississippi State University
Box 9775
Mississippi State, MS 39762



==============================Original Headers==============================
6, 23 -- From ALawrence-at-entomology.msstate.edu Thu Jul 2 08:21:00 2009
6, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120])
6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n62DKweA006515
6, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jul 2009 08:20:59 -0500
6, 23 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197])
6, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n62DKulv003281
6, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL)
6, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jul 2009 08:20:56 -0500
6, 23 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu
6, 23 -- with Novell_GroupWise; Thu, 02 Jul 2009 08:20:56 -0500
6, 23 -- Message-Id: {4A4C6DE2.B26A.00E6.0-at-entomology.msstate.edu}
6, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
6, 23 -- Date: Thu, 02 Jul 2009 08:20:50 -0500
6, 23 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu}
6, 23 -- To: {Microscopy-at-Microscopy.Com}
6, 23 -- Subject: M&M 2009 bursary/volunteer note
6, 23 -- References: {4A4C6D4E020000E600064034-at-mailhost.groupwise.msstate.edu}
6, 23 -- {4A4C6DE2020000E600064037-at-mailhost.groupwise.msstate.edu}
6, 23 -- Mime-Version: 1.0
6, 23 -- Content-Type: text/plain; charset=US-ASCII
6, 23 -- Content-Disposition: inline
6, 23 -- Content-Transfer-Encoding: 8bit
6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n62DKweA006515
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Thu, 2 Jul 2009 11:21:45 -0500
Subject: [Microscopy] Magnetic field

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

It is my experience that you will throw away so much performance that you
really need to find a site that fits the FEI specification. In most
laboratories you find that from the date of installation the environment
tends to degrade, so starting with a problem is not a good idea!

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {bozhilov-at-ucr.edu}
To: {protrain-at-emcourses.com}
Sent: Thursday, July 02, 2009 12:24 AM

Hi Sudhir,

Thank you for your reply.
The general common sense replies I got about cancelation fields,
changing rooms, etc are welcomed but they are not what my question is
about.
I am actually looking for input from someone who has practical
experience with such levels of interference and could tell me the
ranges of magnificaions at which one starts to see problems.

About the problems you mention at 20Kx or so, did you observe the
interferences by yourself and do you know what ranges of magnetic
field did cause it?

Krassimir.
================================


On Jul 2, 2009, at 7:20 AM, Sudhir Sharma wrote:

} Hello,
}
} It's better to change the site immediately or else you would face
} very tough situation in which there will be magnetic interference in
} images even at magnification of 20,000 or even lower.
}
} Sudhir Sharma
}
}
}
}
}
} On Thu, Jul 2, 2009 at 7:24 PM, {protrain-at-emcourses.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi
}
} It is my experience that you will throw away so much performance
} that you
} really need to find a site that fits the FEI specification. In most
} laboratories you find that from the date of installation the
} environment
} tends to degrade, so starting with a problem is not a good idea!
}
} Steve Chapman FRMS
} Senior Consultant
} Protrain for Electron Microscopy Consultancy and Training world wide
} Tel +44 1280816512 Fax +44 1280814007
} Cell +44 7711606967 Web www.emcourses.com
}
}
} ----- Original Message -----
} X-from: {bozhilov-at-ucr.edu}
} To: {protrain-at-emcourses.com}
} Sent: Thursday, July 02, 2009 12:24 AM
} Subject: [Microscopy] Magnetic field
}
}
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } A colleague of mine is preparing to install an FEI Quanta450-FEG.
} The
} } site survey by FEI of the room designated to accommodate the SEM
} } revealed that there is a horizontal magnetic field component which
} is
} } above FEI specs, all other specs for acoustic, electrical, and
} } electromagnetic background were met. The measurements revealed
} } horizontal peak-to-peak magnetic field of 4.3 mGauss whereas the
} specs
} } require 3 mGauss or less.
} }
} } My question is does anyone have experience with such levels of
} } magnetic field strength deviations from the specs and to what extent
} } would this affect the maximum attainable resolution?
} }
} }
} } Krassimir N. Bozhilov
} }
} } University of California
} } Riverside, CA 92521
} }
} } tel: 951 827 2998
} } fax: 951 827 2489
} }
} } bozhilov-at-ucr.edu
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 19 -- From bozhilov-at-ucr.edu Wed Jul 1 18:22:29 2009
} } 9, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu
} [138.23.214.80])
} } 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id
} } n61NMSqh015242
} } 9, 19 -- for {microscopy-at-microscopy.org} ; Wed, 1 Jul 2009 18:22:28
} -0500
} } 9, 19 -- Received: from [138.23.185.168] ([138.23.185.168])
} } 9, 19 -- by sentoku.ucr.edu (MOS 3.10.5-GA)
} } 9, 19 -- with ESMTP id FYR75031 (AUTH bozhilov-at-ucr.edu)
} } 9, 19 -- for {microscopy-at-microscopy.org} ;
} } 9, 19 -- Wed, 1 Jul 2009 16:22:26 -0700 (PDT)
} } 9, 19 -- Message-Id: {00EAA329-DFA6-43E2-8A3A-A941B8F7A2A9-at-ucr.edu}
} } 9, 19 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
} } 9, 19 -- To: microscopy-at-microscopy.org
} } 9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} } delsp=yes
} } 9, 19 -- Content-Transfer-Encoding: 7bit
} } 9, 19 -- Mime-Version: 1.0 (Apple Message framework v935.3)
} } 9, 19 -- Subject: Magnetic field
} } 9, 19 -- Date: Wed, 1 Jul 2009 16:22:26 -0700
} } 9, 19 -- X-Mailer: Apple Mail (2.935.3)
} } 9, 19 -- X-Junkmail-Status: score=0/50, host=
} } ==============================End of -
} } Headers==============================
}
}
} ==============================Original
} Headers==============================
} 8, 30 -- From protrain-at-emcourses.com Thu Jul 2 08:48:30 2009
} 8, 30 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net
} [196.26.208.170])
} 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n62DmTgJ021603
} 8, 30 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jul 2009
} 08:48:29 -0500
} 8, 30 -- Received: from 5ad57283.bb.sky.com ([90.213.114.131]:49932
} helo=StevePC)
} 8, 30 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0)
} 8, 30 -- (envelope-from {protrain-at-emcourses.com} )
} 8, 30 -- id 1MMMeI-0000VT-3d by authid
} {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Thu, 02 Jul
} 2009 15:48:27 +0200
} 8, 30 -- Message-ID: {C7E27A00930E4877B72668F0E8ACBAFE-at-StevePC}
} 8, 30 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com}
} 8, 30 -- From: "Steve Chapman" {protrain-at-emcourses.com}
} 8, 30 -- To: {bozhilov-at-ucr.edu}
} 8, 30 -- Cc: "American EM Soc" {microscopy-at-microscopy.com}
} 8, 30 -- References: {200907012324.n61NOGoP016632-at-ns.microscopy.com}
} 8, 30 -- In-Reply-To: {200907012324.n61NOGoP016632-at-ns.microscopy.com}
} 8, 30 -- Subject: Re: [Microscopy] Magnetic field
} 8, 30 -- Date: Thu, 2 Jul 2009 14:48:23 +0100
} 8, 30 -- Organization: Protrain
} 8, 30 -- MIME-Version: 1.0
} 8, 30 -- Content-Type: text/plain;
} 8, 30 -- format=flowed;
} 8, 30 -- charset="iso-8859-1";
} 8, 30 -- reply-type=original
} 8, 30 -- Content-Transfer-Encoding: 7bit
} 8, 30 -- X-Priority: 3
} 8, 30 -- X-MSMail-Priority: Normal
} 8, 30 -- X-Mailer: Microsoft Windows Mail 6.0.6001.18000
} 8, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.0.6001.18049
} 8, 30 -- X-Scan-Signature: 6976016b80fad038dd08d7eb08e44422{82}}
} 8, 30 -- X-Trace: smtp01.dial-up.net 1MMMeI-0000VT-3d
} 37dcc9f524014c3829d232c9dc9f2c12
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
8, 21 -- From bozhilov-at-ucr.edu Thu Jul 2 11:21:44 2009
8, 21 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n62GLfEV011869
8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jul 2009 11:21:42 -0500
8, 21 -- Received: from [138.23.185.168] ([138.23.185.168])
8, 21 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
8, 21 -- with ESMTP id EXD78922 (AUTH bozhilov-at-ucr.edu);
8, 21 -- Thu, 2 Jul 2009 09:21:38 -0700 (PDT)
8, 21 -- Cc: microscopy-at-microscopy.com
8, 21 -- Message-Id: {1A57DACC-F3CB-4C46-ABA1-6B4C75D8DDEC-at-ucr.edu}
8, 21 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
8, 21 -- To: Sudhir Sharma {sudhir.bcl-at-gmail.com}
8, 21 -- In-Reply-To: {8a8192dd0907020720n8abd0d8l5eb927f683d851f6-at-mail.gmail.com}
8, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 21 -- Content-Transfer-Encoding: 7bit
8, 21 -- Mime-Version: 1.0 (Apple Message framework v935.3)
8, 21 -- Subject: Re: [Microscopy] Re: Magnetic field
8, 21 -- Date: Thu, 2 Jul 2009 09:21:37 -0700
8, 21 -- References: {200907021354.n62DsXEA001493-at-ns.microscopy.com} {8a8192dd0907020720n8abd0d8l5eb927f683d851f6-at-mail.gmail.com}
8, 21 -- X-Mailer: Apple Mail (2.935.3)
8, 21 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================




From: Williams-at-GENECTR.HUNTER.CUNY.EDU
Date: Thu, 2 Jul 2009 12:31:25 -0500
Subject: [Microscopy] Opening To Teach TEM Course at Hunter College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List
Hunter College is looking for an individual to teach a one semester electron microscopy course in the Spring of 2010. This school is located on the upper east side of New York City, at 68th street and Lexington. The class would meet twice a week for lectures and once for a lab. It would be at the introductory level covering  the basic theoretical background of TEM, sample preparation and imaging.  Interested candidates should email their resume to microscopy-at-genectr.hunter.cuny.edu

Thank you

Dr. Lloyd Williams
Dept of Biology
Hunter College
695 Park Ave
New York NY 10065




==============================Original Headers==============================
6, 32 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Thu Jul 2 12:31:24 2009
6, 32 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34])
6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n62HVMUr028862
6, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jul 2009 12:31:23 -0500
6, 32 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by
6, 32 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id
6, 32 -- 8.1.375.2; Thu, 2 Jul 2009 13:31:35 -0400
6, 32 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by
6, 32 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Thu, 2 Jul 2009
6, 32 -- 13:31:54 -0400
6, 32 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU}
6, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
6, 32 -- Date: Thu, 2 Jul 2009 13:31:47 -0400
6, 32 -- Subject: Opening To Teach TEM Course at Hunter College
6, 32 -- Thread-Topic: Opening To Teach TEM Course at Hunter College
6, 32 -- Thread-Index: Acn7OsJbBVG16l4AQDiyuI9sJwxzhQ==
6, 32 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE744B05EFE7-at-Xchange5.bio.hunter.cuny.edu}
6, 32 -- Accept-Language: en-US
6, 32 -- Content-Language: en-US
6, 32 -- X-MS-Has-Attach:
6, 32 -- X-MS-TNEF-Correlator:
6, 32 -- x-cr-puzzleid: {9771D83E-E464-446C-90C5-E52AC769B554}
6, 32 -- x-cr-hashedpuzzle: qlg= AXYm Al6A BKW+ Cmyf EaG+ F9YQ GCRn GTAf HJgV IZ87
6, 32 -- IiJq JH49 J6jj KQYW
6, 32 -- Kln9;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{9771D83E-E464-446C-90C5-E52AC769B554};dwBpAGwAbABpAGEAbQBzAEAAZwBlAG4AZQBjAHQAcgAuAGgAdQBuAHQAZQByAC4AYwB1AG4AeQAuAGUAZAB1AA==;Thu,
6, 32 -- 02 Jul 2009 17:31:47
6, 32 -- GMT;TwBwAGUAbgBpAG4AZwAgAFQAbwAgAFQAZQBhAGMAaAAgAFQARQBNACAAQwBvAHUAcgBzAGUAIABhAHQAIABIAHUAbgB0AGUAcgAgAEMAbwBsAGwAZQBnAGUA
6, 32 -- acceptlanguage: en-US
6, 32 -- Content-Type: text/plain; charset="iso-8859-1"
6, 32 -- MIME-Version: 1.0
6, 32 -- Content-Transfer-Encoding: 8bit
6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n62HVMUr028862
==============================End of - Headers==============================




From: mjamison-at-caissonlabs.com
Date: Thu, 2 Jul 2009 18:24:37 -0500
Subject: [Microscopy] viaWWW: paraffin embedding with protein and lipid staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mjamison-at-caissonlabs.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mjamison-at-caissonlabs.com
Name: Michelle Jamison

Organization: Caisson Labs

Title-Subject: [Filtered] paraffin embedding with protein and lipid staining

Question: Hi all
I have been using Polyethylene Glycol to embed fresh sorghum seed,
10-20 days after pollination. My ultimate goal is to stain for
protein and lipids. I have had sucess with this method, but would
like to use parafin instead due to the beautiful sections obtainable
with this method, and ease of use.
I hesitate to use this due to problems of fixation (formaldehyde,
acetic acid ethanol), dehydration (ethanol), and prolonged heat. I
understand that these cause denatureation of protein and lipid
breakdown.
Does anyone have experience processing plant material in the way I
would like to? Any input woudl be most appreciated.
Thanks!
...One of the Privileged who gets to see Small Things!
Michelle

Login Host: 129.123.46.133
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jul 2 18:24:37 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n62NOZjR022346
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jul 2009 18:24:37 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c672f2225d45-at-[206.69.208.22]}
6, 11 -- Date: Thu, 2 Jul 2009 18:24:35 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: mjamison-at-caissonlabs.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: paraffin embedding with protein and lipid staining
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rra-at-stowers.org
Date: Thu, 2 Jul 2009 18:25:08 -0500
Subject: [Microscopy] viaWWW: Cryo-Sectioning with trough

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rra-at-stowers.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rra-at-stowers.org
Name: Rhonda Trimble

Organization: Stowers Institute

Title-Subject: [Filtered] Cryo-Sectioning with trough

Question: Good Afternoon to you all! I have a question for those who
may have experience doing ultra cryo-sectioning with a trough, water
& DMSO? We are only using a cryo-dry knife.

The cryo-knife diamond with a trough is offered through Diatome, and
I am wondering what applications this is good for? Does anyone have
experience with this?

Thanks in advance, and may everyone have a "not to explosive" 4th of
July holiday!

Rhonda Trimble
rra-at-stowers.org

Login Host: 204.56.6.101
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Jul 2 18:25:07 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n62NP72p022928
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jul 2009 18:25:07 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c672f23c6346-at-[206.69.208.22]}
9, 11 -- Date: Thu, 2 Jul 2009 18:25:06 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: rra-at-stowers.org (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Cryo-Sectioning with trough
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Thu, 2 Jul 2009 19:08:23 -0500
Subject: [Microscopy] Re: viaWWW: Cryo-Sectioning with trough

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Rhonda,

I guess those who reply to your question have little illusions about
their age.... ;-)

I do have experience with "wet" cryosectioning according to the
Kuhlmann and Bernhard technique of the late 60's, early 70's which
used glycerol/water or DMSO/water mixtures. I guess the methods
developed because they compared more or less to sectioning at room
temperature, facilitating section pick up with marinozzi rings etc.
With the development of the Tokuyasu technique in 1973, the "wet"
approach was largely abandoned. Some reasons: trough liquids at
temperatures around -50°C are very viscous and cause sections to be
severely compressed as they hit the syrupy liquid surface. Sections
did not really float the way plastic ultrathins do on water, but they
were always halfway submerged and it is very hard if not impossible to
judge section quality. But with the equipment available at that time,
that was hardly the issue. I remember being happy to have a few decent
sections once every few days on the old unmodified Reichert FC-2!

Cheers, I don't think the 4th of July will be very explosive in New
Zealand!

Jan Leunissen


On 3/07/2009, at 11:25 AM, rra-at-stowers.org wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both rra-at-stowers.org as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: rra-at-stowers.org
} Name: Rhonda Trimble
}
} Organization: Stowers Institute
}
} Title-Subject: [Filtered] Cryo-Sectioning with trough
}
} Question: Good Afternoon to you all! I have a question for those who
} may have experience doing ultra cryo-sectioning with a trough, water
} & DMSO? We are only using a cryo-dry knife.
}
} The cryo-knife diamond with a trough is offered through Diatome, and
} I am wondering what applications this is good for? Does anyone have
} experience with this?
}
} Thanks in advance, and may everyone have a "not to explosive" 4th of
} July holiday!
}
} Rhonda Trimble
} rra-at-stowers.org


==============================Original Headers==============================
9, 21 -- From leunissen-at-aurion.nl Thu Jul 2 19:08:22 2009
9, 21 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6308LbL019527
9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jul 2009 19:08:22 -0500
9, 21 -- Received: from [192.168.1.50] (really [122.57.246.3]) by fep01.xtra.co.nz
9, 21 -- with ESMTP
9, 21 -- id {20090703000819.BTFT3703.fep01.xtra.co.nz-at-[192.168.1.50]} ;
9, 21 -- Fri, 3 Jul 2009 12:08:19 +1200
9, 21 -- Cc: rra-at-stowers.org
9, 21 -- Message-Id: {73F665AB-1C7D-464B-8F9B-43A94C63C009-at-aurion.nl}
9, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl}
9, 21 -- To: microscopy-at-microscopy.com
9, 21 -- In-Reply-To: {200907022325.n62NPK5W023487-at-ns.microscopy.com}
9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes
9, 21 -- Mime-Version: 1.0 (Apple Message framework v935.3)
9, 21 -- Subject: Re: [Microscopy] viaWWW: Cryo-Sectioning with trough
9, 21 -- Date: Fri, 3 Jul 2009 12:08:18 +1200
9, 21 -- References: {200907022325.n62NPK5W023487-at-ns.microscopy.com}
9, 21 -- X-Mailer: Apple Mail (2.935.3)
9, 21 -- Content-Transfer-Encoding: 8bit
9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6308LbL019527
==============================End of - Headers==============================




From: yngli-at-ucdavis.edu
Date: Fri, 3 Jul 2009 08:07:13 -0500
Subject: [Microscopy] viaWWW: convergence angle and acceptance angle for EELS in STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yngli-at-ucdavis.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yngli-at-ucdavis.edu
Name: Ying Li

Organization: University of California Davis

Title-Subject: [Filtered] The convergence angle and acceptance angle
for EELS in STEM mode with JEM 2500 TEM

Question: Hi, all,
How to get the convergence angle and acceptance angle values for EELS
in STEM mode with the JEOL JEM2500 SE TEM? Before I done EELS with
FEI TECNAI F30, the acceptance angle value is the radius of the
entrance aperture EELS spectrometer divided the distance between
specimen and the entrance aperture EELS spectrometer. And convergence
angle is the radius of diffraction disk of the CBED patterns divided
the camera length in which you got the CBED.
Are these methods still applicable for JEM 2500 TEM?
And for camera length is just the showing camera length when you do
CBED or need get the value from the Gatan engineer? There is some
difference for EELS quantitative analysis with STEM mode and TEM mode?

Thanks a lot!


Best regards
Ying Li(Y. Li)
------------------------------------------------------------------------------------------------------------
Department of Chemical Engineering and Materials Science
University of California, Davis,
One Shields Avenue,Davis, CA 95616
Phone:530-752-9568; email:yngli-at-ucdavis.edu


Login Host: 169.237.204.120
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Fri Jul 3 08:07:11 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n63D789s020492
10, 12 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jul 2009 08:07:10 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240800c673b2ae7ed8-at-[206.69.208.22]}
10, 12 -- Date: Fri, 3 Jul 2009 08:07:08 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: yngli-at-ucdavis.edu (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: convergence angle and acceptance angle for EELS in STEM
10, 12 -- mode with JEM 2500
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ray.twesten-at-sbcglobal.net
Date: Fri, 3 Jul 2009 12:29:57 -0500
Subject: [Microscopy] viaWWW: convergence angle and acceptance angle for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ying Li,

Your basic analysis is correct. The collection angle is governed by the
actual camera length at the entrance aperture to the spectrometer. This is
typically given by the indicated camera length times a correction factor
related to the difference between the diffraction camera plane and the
entrance aperture to the spectrometer. However, in STEM mode, the indicated
camera length is often not well calibrated or simply not listed.

On the Gatan website, there is a recent article describing the method of
actually measuring the convergence and collection angles of the system at a
large range of settings and operating mode. The URL is:
|--http://www.gatan.com/resources/knowhow/kh19_eels.php--|

By actually measuring the values at your operating conditions, you can be
sure the values are accurate.

Regarding "There is some difference for EELS quantitative analysis with STEM
mode and TEM mode?". In principle, there is no difference as long as you
correctly measure the convergence and collection angles in both modes. In
practice, there can be subtle operational differences.

In STEM mode, the analysis area is fixed by the probes size. The convergence
angle is fixed by the condenser aperture and the probe settings. Also, you
will always have a diffraction pattern entering the spectrometer (aka, image
coupled mode) so the collection angle is well defined.

In TEM mode, you can be in either diffraction or image mode.
In diffraction mode (aka, image coupled mode), you have a diffraction
pattern entering the spectrometer so the collection angle is fixed, but the
illumination area and convergence angles can vary a great deal. If you are
defining the illuminated area with a converged beam, you are the same as the
STEM case. If you are working in a selected area diffraction mode, the
convergence angle will be quite variable. Fortunately, as long as it is
small compared to the collection angle, the exact value is not that
important. The analyzed area in this mode will be defined by the selected
area aperture. Due to lens aberrations, this area will change as a function
of energy loss so it will be hard to define a small area.

In image mode (aka, diffraction coupled mode), the collection angle is
defined by the objective aperture. The analysis area is defined by the
spectrometer entrance aperture. Due mainly to chromatic aberration, this
area will be a strong function of energy loss. If the sample is uniform in
composition and thickness, this is not such an issue. If the sample is
changing rapidly in the region of the entrance aperture, this can cause
major problems.

(see Egerton 2nd ed., p 69-76)

Hope this helps.

Best regards,
Ray


Ray D. Twesten, Ph.D.
Product Manager – Analytical Instruments
  Gatan, Inc.
Tel. +1 (925) 224-7392

Disclaimer: Gatan manufactures energy-loss spectrometers and imaging
filters. We have a vested interest in everyone getting great EELS data!


-----Original Message-----
X-from: yngli-at-ucdavis.edu [mailto:yngli-at-ucdavis.edu]
Sent: Friday, July 03, 2009 6:22 AM
To: ray.twesten-at-sbcglobal.net

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yngli-at-ucdavis.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yngli-at-ucdavis.edu
Name: Ying Li

Organization: University of California Davis

Title-Subject: [Filtered] The convergence angle and acceptance angle
for EELS in STEM mode with JEM 2500 TEM

Question: Hi, all,
How to get the convergence angle and acceptance angle values for EELS
in STEM mode with the JEOL JEM2500 SE TEM? Before I done EELS with
FEI TECNAI F30, the acceptance angle value is the radius of the
entrance aperture EELS spectrometer divided the distance between
specimen and the entrance aperture EELS spectrometer. And convergence
angle is the radius of diffraction disk of the CBED patterns divided
the camera length in which you got the CBED.
Are these methods still applicable for JEM 2500 TEM?
And for camera length is just the showing camera length when you do
CBED or need get the value from the Gatan engineer? There is some
difference for EELS quantitative analysis with STEM mode and TEM mode?

Thanks a lot!


Best regards
Ying Li(Y. Li)
----------------------------------------------------------------------------
--------------------------------
Department of Chemical Engineering and Materials Science
University of California, Davis,
One Shields Avenue,Davis, CA 95616
Phone:530-752-9568; email:yngli-at-ucdavis.edu


Login Host: 169.237.204.120
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Fri Jul 3 08:07:11 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n63D789s020492
10, 12 -- for |--microscopy-at-microscopy.com--|; Fri, 3 Jul 2009
08:07:10
-0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: |--p06240800c673b2ae7ed8-at-[206.69.208.22]--|
10, 12 -- Date: Fri, 3 Jul 2009 08:07:08 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: yngli-at-ucdavis.edu (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: convergence angle and acceptance angle for EELS
in STEM
10, 12 -- mode with JEM 2500
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
33, 23 -- From rtwesten-at-gatan.com Fri Jul 3 12:27:38 2009
33, 23 -- Received: from smtp.gatan.com (smtp.gatan.com [209.3.42.249])
33, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n63HRcaZ015463
33, 23 -- for |--microscopy-at-microscopy.com--|; Fri, 3 Jul 2009
12:27:38 -0500
33, 23 -- Received: from TWESTEDLTE6400 ([10.13.7.105]) by smtp.gatan.com
with Microsoft SMTPSVC(5.0.2195.6713);
33, 23 -- Fri, 3 Jul 2009 10:30:02 -0700
33, 23 -- From: "Ray Twesten" |--rtwesten-at-gatan.com--|
33, 23 -- To: |--microscopy-at-microscopy.com--|
33, 23 -- Cc: |--yngli-at-ucdavis.edu--|
33, 23 -- References: |--200907031322.n63DM07x000476-at-ns.microscopy.com--|
33, 23 -- In-Reply-To: |--200907031322.n63DM07x000476-at-ns.microscopy.com--|
33, 23 -- Subject: RE: [Microscopy] viaWWW: convergence angle and acceptance
angle for EELS in STEM
33, 23 -- Date: Fri, 3 Jul 2009 10:27:33 -0700
33, 23 -- Message-ID: |--040101c9fc03$8ce21ce0$a6a656a0$-at-com--|
33, 23 -- MIME-Version: 1.0
33, 23 -- Content-Type: text/plain;
33, 23 -- charset="iso-8859-1"
33, 23 -- X-Mailer: Microsoft Office Outlook 12.0
33, 23 -- Thread-Index: Acn74UDKizftkkUWQiqGVnbOV/EMSAAG5i2g
33, 23 -- Content-Language: en-us
33, 23 -- X-OriginalArrivalTime: 03 Jul 2009 17:30:02.0262 (UTC)
FILETIME=[E585C360:01C9FC03]
33, 23 -- Content-Transfer-Encoding: 8bit
33, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n63HRcaZ015463
==============================End of - Headers==============================



==============================Original Headers==============================
35, 29 -- From ray.twesten-at-sbcglobal.net Fri Jul 3 12:29:57 2009
35, 29 -- Received: from smtp103.sbc.mail.gq1.yahoo.com (smtp103.sbc.mail.gq1.yahoo.com [67.195.15.62])
35, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n63HTrtO015574
35, 29 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jul 2009 12:29:55 -0500
35, 29 -- Received: (qmail 71344 invoked from network); 3 Jul 2009 17:29:52 -0000
35, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
35, 29 -- s=s1024; d=sbcglobal.net;
35, 29 -- h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:From:To:References:In-Reply-To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language;
35, 29 -- b=IlBbpEi6+4os9p3qJAXJQwsu55rVTh2EKLAZoqAB8MqyrhT0qLsiUzWyUQU8agAo5OWsGngnc7czHqrwf13s95razdu5ra7YAgGZub+9f07gXQhmdW6r4/R5wQrgCEFyreU9q8HcH9zW5BpCtY7aQodAiM3GdeijZzBHslic2pE= ;
35, 29 -- Received: from unknown (HELO TWESTEDLTE6400) (ray.twesten-at-209.3.42.11 with login)
35, 29 -- by smtp103.sbc.mail.gq1.yahoo.com with SMTP; 3 Jul 2009 17:29:51 -0000
35, 29 -- X-Yahoo-SMTP: fycHU2aswBByXb8EnYI7o6SmqB8qZEbNW53UkXuunfjk8OMjORM-
35, 29 -- X-YMail-OSG: nTD7rBQVM1krd3YiIGGAbUt4RMzVbSaZSkeNc3cHVqpIj4G6eOZzGHXtNklrn.io1_eSNhEONKD4oz6olUvVfaRTcz5D8kQUCuO2IbKNQQ0zxciCs0CejfgKb.9qUQ1q9_1YqG.4pgYKr7NOgLMkk3i26YNd52lFnBiuXPk.NED3lxQwwhyREFYWKb1Ssqfjemj1azCu7gczximV_X.A3atrHnABahan_xuPwSv07gGToaOLYAaFL9MsKvqjYiWSuIl5wotsESgtCM0u_OZ_PgDaExMCwFY8pcGlrTKPczmk3Kwh
35, 29 -- X-Yahoo-Newman-Property: ymail-3
35, 29 -- From: "Ray Twesten" {ray.twesten-at-sbcglobal.net}
35, 29 -- To: {microscopy-at-microscopy.com}
35, 29 -- References: {200907031727.n63HRdjQ015466-at-ns.microscopy.com}
35, 29 -- In-Reply-To: {200907031727.n63HRdjQ015466-at-ns.microscopy.com}
35, 29 -- Subject: RE: viaWWW: convergence angle and acceptance angle for EELS in STEM
35, 29 -- Date: Fri, 3 Jul 2009 10:29:51 -0700
35, 29 -- Message-ID: {040201c9fc03$df33dfb0$9d9b9f10$-at-twesten-at-sbcglobal.net}
35, 29 -- MIME-Version: 1.0
35, 29 -- Content-Type: text/plain;
35, 29 -- charset="iso-8859-1"
35, 29 -- X-Mailer: Microsoft Office Outlook 12.0
35, 29 -- Thread-Index: Acn8A5AhYzWQYGHgS5WjmV0nJjreYgAACGmg
35, 29 -- Content-Language: en-us
35, 29 -- Content-Transfer-Encoding: 8bit
35, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n63HTrtO015574
==============================End of - Headers==============================




From: Williams-at-GENECTR.HUNTER.CUNY.EDU
Date: Mon, 6 Jul 2009 15:40:54 -0500
Subject: [Microscopy] Opening To Teach TEM Course at Hunter College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List
Hunter College is looking for an individual to teach a one semester electron microscopy course in the Spring of 2010. This school is located on the upper east side of New York City, at 68th street and Lexington. The class would meet twice a week for lectures and once for a lab. It would be at the introductory level covering  the basic theoretical background of TEM, sample preparation and imaging. The position would be at the level of an adjunct faculty appointment. Interested candidates should email their resume to microscopy-at-genectr.hunter.cuny.edu
Note I am resubmitting this as there were problems with the above E-mail address receiving E-mail before.

Thank you

Dr. Lloyd Williams
Dept of Biology
Hunter College
695 Park Ave
New York NY 10065




==============================Original Headers==============================
6, 32 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Mon Jul 6 15:40:54 2009
6, 32 -- Received: from genectr.hunter.cuny.edu (genectr.hunter.cuny.edu [146.95.150.34])
6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n66KeqIt005374
6, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jul 2009 15:40:53 -0500
6, 32 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by
6, 32 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id
6, 32 -- 8.1.375.2; Mon, 6 Jul 2009 16:40:57 -0400
6, 32 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by
6, 32 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Mon, 6 Jul 2009
6, 32 -- 16:41:23 -0400
6, 32 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU}
6, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
6, 32 -- Date: Mon, 6 Jul 2009 16:41:18 -0400
6, 32 -- Subject: Opening To Teach TEM Course at Hunter College
6, 32 -- Thread-Topic: Opening To Teach TEM Course at Hunter College
6, 32 -- Thread-Index: Acn7OsJbBVG16l4AQDiyuI9sJwxzhQ==
6, 32 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE744B05F172-at-Xchange5.bio.hunter.cuny.edu}
6, 32 -- Accept-Language: en-US
6, 32 -- Content-Language: en-US
6, 32 -- X-MS-Has-Attach:
6, 32 -- X-MS-TNEF-Correlator:
6, 32 -- x-cr-puzzleid: {82FF31FC-87B8-47F4-B144-4E723A9B6C7A}
6, 32 -- x-cr-hashedpuzzle: AHSQ A8Gs BP3u CwMy DVdN Dqip FZGZ F4mI F+Ov HMSR IT7Q
6, 32 -- Io18 Ix7B KHky KvKM
6, 32 -- K07i;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{82FF31FC-87B8-47F4-B144-4E723A9B6C7A};dwBpAGwAbABpAGEAbQBzAEAAZwBlAG4AZQBjAHQAcgAuAGgAdQBuAHQAZQByAC4AYwB1AG4AeQAuAGUAZAB1AA==;Mon,
6, 32 -- 06 Jul 2009 20:41:18
6, 32 -- GMT;TwBwAGUAbgBpAG4AZwAgAFQAbwAgAFQAZQBhAGMAaAAgAFQARQBNACAAQwBvAHUAcgBzAGUAIABhAHQAIABIAHUAbgB0AGUAcgAgAEMAbwBsAGwAZQBnAGUA
6, 32 -- acceptlanguage: en-US
6, 32 -- Content-Type: text/plain; charset="iso-8859-1"
6, 32 -- MIME-Version: 1.0
6, 32 -- Content-Transfer-Encoding: 8bit
6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n66KeqIt005374
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Tue, 7 Jul 2009 06:16:24 -0500
Subject: [Microscopy] Sem stereo images question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all
I have a student who wishes to do some stereo images, which I have no
experience of. Could you tell me the best software to use to merge the 2
tilted images. Or some good references.

Many thanks
Nikki

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
5, 35 -- From Nicola.Weston-at-nottingham.ac.uk Tue Jul 7 06:16:23 2009
5, 35 -- Received: from ixe-mta-23.emailfiltering.com (ixe-mta-23-tx.emailfiltering.com [194.116.199.156])
5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67BGLLD000495
5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 06:16:22 -0500
5, 35 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4])
5, 35 -- by ixe-mta-23.emailfiltering.com with emfmta (version 3.7.1.44.1.r-3.2.3-libc2.3.2) vanilla id 12871378
5, 35 -- for microscopy-at-microscopy.com; Tue, 07 Jul 2009 12:16:19 +0100
5, 35 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10])
5, 35 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60)
5, 35 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 35 -- id 1MO8eL-0007yU-GT
5, 35 -- for microscopy-at-microscopy.com; Tue, 07 Jul 2009 12:15:49 +0100
5, 35 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 35 -- Tue, 7 Jul 2009 12:15:37 +0100
5, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 35 -- Content-class: urn:content-classes:message
5, 35 -- MIME-Version: 1.0
5, 35 -- Content-Type: text/plain;
5, 35 -- charset="us-ascii"
5, 35 -- Subject: Sem stereo images question
5, 35 -- Date: Tue, 7 Jul 2009 12:15:37 +0100
5, 35 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D9401C0C36C-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 35 -- X-MS-Has-Attach:
5, 35 -- X-MS-TNEF-Correlator:
5, 35 -- Thread-Topic: Sem stereo images question
5, 35 -- Thread-Index: Acn+9EEgtBVFBbnZRyCy+0OFqU4YFw==
5, 35 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 35 -- To: {microscopy-at-microscopy.com}
5, 35 -- X-OriginalArrivalTime: 07 Jul 2009 11:15:37.0787 (UTC) FILETIME=[414DE8B0:01C9FEF4]
5, 35 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
5, 35 -- X-UoN-MailScanner: Found to be clean
5, 35 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 35 -- X-Spam-Status: No
5, 35 -- Content-Transfer-Encoding: 8bit
5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67BGLLD000495
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 7 Jul 2009 09:41:57 -0500
Subject: [Microscopy] Question: AFM to measure affinity forces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all!

I would have questions concerning the measurement of affinity forces by AFM.
My first questions are
1) is it possible to measure such forces in a liquid environment (like a physiological buffer)?
2) Is it a relative quantification or an absolute quantification?
3) On which scale is the affinity expressed?
4) Is a calibration necessary? How is it done?

Further questions should better be asked in private emails.

Best regards,

Stephane (did you notice there is no "i"?)





==============================Original Headers==============================
9, 24 -- From nizets2-at-yahoo.com Tue Jul 7 09:41:57 2009
9, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n67Efuap022759
9, 24 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 09:41:57 -0500
9, 24 -- Received: (qmail 3606 invoked by uid 60001); 7 Jul 2009 14:41:55 -0000
9, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1246977715; bh=IV3Ce1LfBaysDvtL86cm/PhkQWARqQjQbtOOIkmhrfE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=Ag6xjjud3VH4rrk7RBRlkptaB6u75bRSTU7+tWUl2LbuE/pSr1DaZGDmgpoz7gfmJaJ2mb6hMoUDGX+6yzjHz/rXv1QbURDzqamDSJGd4pJtZEy5gZv6TD1gprGOeRU7LuSY60dgeGjdTlJaosrIvCJ4K9l+lDcbOOEONIA6loQ=
9, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
9, 24 -- s=s1024; d=yahoo.com;
9, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
9, 24 -- b=b5oCWl1wJvVQlOrxd6QBOUUTlK6HgaKwKDRbNLilGH+m7GCr5XLX7tyDjN93KfN645N4yyRJLHPnqh+q5SJ7v6qFPEuv/1M9jphqxI4O81VMfL2Rr+LSKgGNxRv9yzk0aSWulXwscm04212cw1RbRUetnVd9EjXtIM1MbnmWfNg=;
9, 24 -- Message-ID: {604816.1893.qm-at-web110801.mail.gq1.yahoo.com}
9, 24 -- X-YMail-OSG: 0oLGJHoVM1nfLnqJIOPpfwadHPXDk6S2xsYBP30oWyDZmTsw1kDB66oPGWOH1f6ogeTf8c1a3mMJFsXKhXQ0xUviOtj_wi.NSnV.g1FjZcN0v5lt7mNUHlZwWpcNPIgWH9PRUNa4yXd5sJtjT8.zs7_8GaAJSH2aGnYMql0L6QPxg6tCnCum4VBz4oNEGGu4RpFz6Q5dsM_F_VjLUSRP5xCbIDL6DDjbRL50g71qf4SVgq0lZnnyKESsWjYP5Kw6GoV4gVDcPIN8RR_Z8_lnPfvU0e7u..oBHCmGxBhJ_F1TgNPtRHmmcB07s3b_DC.LPw8Fk947
9, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Tue, 07 Jul 2009 07:41:55 PDT
9, 24 -- X-Mailer: YahooMailRC/1357.18 YahooMailWebService/0.7.289.15
9, 24 -- References: {200907071127.n67BR4h8007095-at-ns.microscopy.com}
9, 24 -- Date: Tue, 7 Jul 2009 07:41:55 -0700 (PDT)
9, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
9, 24 -- Subject: Question: AFM to measure affinity forces
9, 24 -- To: microscopy-at-microscopy.com
9, 24 -- In-Reply-To: {200907071127.n67BR4h8007095-at-ns.microscopy.com}
9, 24 -- MIME-Version: 1.0
9, 24 -- Content-Type: text/plain; charset=iso-8859-1
9, 24 -- Content-Transfer-Encoding: 8bit
9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67Efuap022759
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Tue, 7 Jul 2009 12:38:01 -0500
Subject: [Microscopy] Re: price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, We plan to purchase new transmission electron microscope and our
budget is S 150,000 (This is for only biological samples). Is it
possible for us to get one with that price range?. Please let us know
if any one recently bought one. Your help is appreciated.

Sincerely
V.Kabilan
UMDNJ-NJDS
New Jersey-NJ


==============================Original Headers==============================
3, 26 -- From velliyka-at-umdnj.edu Tue Jul 7 12:38:01 2009
3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n67Hc0kr013985
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 12:38:01 -0500
3, 26 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 88BBB10B7FD
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:38:00 -0400 (EDT)
3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
3, 26 -- by zix03.umdnj.edu (Proprietary) with ESMTP id AB7ED10B7C1
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:37:59 -0400 (EDT)
3, 26 -- Received: from ([10.32.15.167])
3, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.19170237;
3, 26 -- Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- MIME-version: 1.0
3, 26 -- Content-transfer-encoding: 7BIT
3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
3, 26 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
3, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KMF00298AB8TP30-at-umduwc01.umdnj.edu} for
3, 26 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 13:37:56 -0400 (EDT)
3, 26 -- Message-id: {d471a6153f3c62244cb90f9ff3d95136-at-umdnj.edu}
3, 26 -- To: Microscopy-at-microscopy.com
3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
3, 26 -- Subject: Re: price for new Transmission electron microscope
3, 26 -- Date: Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Tue, 7 Jul 2009 12:46:25 -0500
Subject: [Microscopy] Re: price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just sat on an NIH S10 study section dealing with funding requests for
new EMs. The requests ranged from 400K to just over 7 million. I don't
know for sure but my guess is that it would be hard to get much lower
400K - maybe 300K without any options like a digital camera but I doubt
it.

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Chair, MU Faculty Council
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


-----Original Message-----
X-from: velliyka-at-umdnj.edu [mailto:velliyka-at-umdnj.edu]
Sent: Tuesday, July 07, 2009 12:39 PM
To: Phillips, Thomas E.

Hi, We plan to purchase new transmission electron microscope and our
budget is S 150,000 (This is for only biological samples). Is it
possible for us to get one with that price range?. Please let us know
if any one recently bought one. Your help is appreciated.

Sincerely
V.Kabilan
UMDNJ-NJDS
New Jersey-NJ


==============================Original
Headers==============================
3, 26 -- From velliyka-at-umdnj.edu Tue Jul 7 12:38:01 2009
3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU
[130.219.34.126])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n67Hc0kr013985
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
12:38:01 -0500
3, 26 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id
88BBB10B7FD
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
13:38:00 -0400 (EDT)
3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
3, 26 -- by zix03.umdnj.edu (Proprietary) with ESMTP id
AB7ED10B7C1
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
13:37:59 -0400 (EDT)
3, 26 -- Received: from ([10.32.15.167])
3, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.19170237;
3, 26 -- Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- MIME-version: 1.0
3, 26 -- Content-transfer-encoding: 7BIT
3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
3, 26 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server
6.3-6.03 (built
3, 26 -- Mar 14 2008; 32bit)) with ESMTP id
{0KMF00298AB8TP30-at-umduwc01.umdnj.edu} for
3, 26 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 13:37:56 -0400
(EDT)
3, 26 -- Message-id: {d471a6153f3c62244cb90f9ff3d95136-at-umdnj.edu}
3, 26 -- To: Microscopy-at-microscopy.com
3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
3, 26 -- Subject: Re: price for new Transmission electron microscope
3, 26 -- Date: Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of -
Headers==============================


==============================Original Headers==============================
13, 29 -- From PhillipsT-at-missouri.edu Tue Jul 7 12:46:25 2009
13, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67HkPxh027188
13, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 12:46:25 -0500
13, 29 -- X-IronPort-Anti-Spam-Filtered: true
13, 29 -- X-IronPort-Anti-Spam-Result: ApoEADUnU0rRauUo/2dsb2JhbADADgEJhzqITQKCVoE7BYE6h18
13, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
13, 29 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Jul 2009 12:46:10 -0500
13, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
13, 29 -- Tue, 7 Jul 2009 12:46:10 -0500
13, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5
13, 29 -- Content-class: urn:content-classes:message
13, 29 -- MIME-Version: 1.0
13, 29 -- Content-Type: text/plain;
13, 29 -- charset="us-ascii"
13, 29 -- Subject: RE: [Microscopy] Re: price for new Transmission electron microscope
13, 29 -- Date: Tue, 7 Jul 2009 12:46:08 -0500
13, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD06EE93C3-at-UM-XMAIL06.um.umsystem.edu}
13, 29 -- In-Reply-To: {200907071738.n67HcxZm015081-at-ns.microscopy.com}
13, 29 -- X-MS-Has-Attach:
13, 29 -- X-MS-TNEF-Correlator:
13, 29 -- Thread-Topic: [Microscopy] Re: price for new Transmission electron microscope
13, 29 -- Thread-Index: Acn/KdBpr20fsDFdRGG0pGzohy/yfAAAHQ3w
13, 29 -- References: {200907071738.n67HcxZm015081-at-ns.microscopy.com}
13, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
13, 29 -- To: {velliyka-at-umdnj.edu} , {Microscopy-at-microscopy.com}
13, 29 -- X-OriginalArrivalTime: 07 Jul 2009 17:46:10.0820 (UTC) FILETIME=[D07AFC40:01C9FF2A]
13, 29 -- Content-Transfer-Encoding: 8bit
13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67HkPxh027188
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 7 Jul 2009 13:11:32 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think they are more expensive than $150,000 US. You could probably buy a used one in that price range.

See what the rest of the listers say. I'd be curious to know the price of a new TEM myself. It will help justify keeping my old one up a running under service contract.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.


--- On Tue, 7/7/09, velliyka-at-umdnj.edu {velliyka-at-umdnj.edu} wrote:

} From: velliyka-at-umdnj.edu {velliyka-at-umdnj.edu}
} Subject: [Microscopy] Re: price for new Transmission electron microscope
} To: vapatpxs-at-yahoo.com
} Date: Tuesday, July 7, 2009, 5:45 PM
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi, We plan to purchase new transmission electron
} microscope and our
} budget is S 150,000 (This is for only biological samples).
} Is it
} possible for us to get one with that price range?. Please
} let us know
} if any one recently bought one. Your help is appreciated.
}
} Sincerely
} V.Kabilan
} UMDNJ-NJDS
} New Jersey-NJ
}
}
} ==============================Original
} Headers==============================
} 3, 26 -- From velliyka-at-umdnj.edu
} Tue Jul  7 12:38:01 2009
} 3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU
} [130.219.34.126])
} 3, 26 --     by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with SMTP id n67Hc0kr013985
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue, 7 Jul 2009 12:38:01 -0500
} 3, 26 -- Received: from zix03.umdnj.edu (ZixVPM
} [127.0.0.1])
} 3, 26 --     by Outbound.umdnj.edu
} (Proprietary) with ESMTP id 88BBB10B7FD
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue,  7 Jul 2009 13:38:00 -0400 (EDT)
} 3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
} 3, 26 --     by zix03.umdnj.edu
} (Proprietary) with ESMTP id AB7ED10B7C1
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue,  7 Jul 2009 13:37:59 -0400 (EDT)
} 3, 26 -- Received: from ([10.32.15.167])
} 3, 26 --     by imail.umdnj.edu with
} ESMTP  id 8XSJWG1.19170237;
} 3, 26 --     Tue, 07 Jul 2009 13:37:56
} -0400
} 3, 26 -- MIME-version: 1.0
} 3, 26 -- Content-transfer-encoding: 7BIT
} 3, 26 -- Content-type: text/plain; charset=US-ASCII;
} format=flowed
} 3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
} 3, 26 --  by umduwc01.umdnj.edu (Sun Java(tm) System
} Messaging Server 6.3-6.03 (built
} 3, 26 --  Mar 14 2008; 32bit)) with ESMTP id {0KMF00298AB8TP30-at-umduwc01.umdnj.edu}
} for
} 3, 26 --  Microscopy-at-microscopy.com;
} Tue, 07 Jul 2009 13:37:56 -0400 (EDT)
} 3, 26 -- Message-id: {d471a6153f3c62244cb90f9ff3d95136-at-umdnj.edu}
} 3, 26 -- To: Microscopy-at-microscopy.com
} 3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
} 3, 26 -- Subject: Re:  price for new Transmission
} electron microscope
} 3, 26 -- Date: Tue, 07 Jul 2009 13:37:56 -0400
} 3, 26 -- X-Mailer: Apple Mail (2.624)
} ==============================End of -
} Headers==============================
}





==============================Original Headers==============================
12, 22 -- From vapatpxs-at-yahoo.com Tue Jul 7 13:11:32 2009
12, 22 -- Received: from web46115.mail.sp1.yahoo.com (web46115.mail.sp1.yahoo.com [68.180.199.132])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n67IBTuC011368
12, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 7 Jul 2009 13:11:30 -0500
12, 22 -- Received: (qmail 94472 invoked by uid 60001); 7 Jul 2009 18:11:27 -0000
12, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1246990287; bh=NEnlL7bPvzlGPzPGImDCpqt7LaiZ6Yw3NcLJqLk3lvg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=OjzAC+Gga5FnHCiZsEGkEpPg36/VmgDbsA55ijGwynL09YN+mRA+qhrsowCsTD/Ka+yF1b4LoSjXqkfKkKi0WKSznmVrDEKcnNXZszYuIK2M1IR6X8R2d659cyiWhCyN7o2Ns8EL++iEhiENuQCHMJirXdgn6CDG4mZpq7YHAt0=
12, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 22 -- s=s1024; d=yahoo.com;
12, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
12, 22 -- b=3lr1C/tDUzzQPxkwqmU5YZ2QFDZSOltPXM+jzCyS7vzgnHX2+oA8OtuOlT7d//KhS8N9Qeuzr5WAu+eMZ295w0fFuSiLoqYtg8j4FmT78FvHOVFGvYrKthWVwm2+06OfWkYMhIT9zBKeGbPOQdzF/TL7rR9oup68x/rdRPRBAAA=;
12, 22 -- Message-ID: {402715.92647.qm-at-web46115.mail.sp1.yahoo.com}
12, 22 -- X-YMail-OSG: VraqIowVM1kh2qMZ7aakmafDLH8sP4mw7ah6tVhDu9iKbOEKTekel3pG41xL8dr2rPoeO.dLBctJPS8iFFdQDMy65ofobq7LDkjMJowqYDQx8U2jRij3fq_Jy6KwMZj1g3.AgOGsrkZT3kCIVGKXoMQkVT0HxbdzCNjT7.fLQI8DSLdlqZl2xba9oWNRqMVBN94VYQ7H7eZPrhAzyVI7ue2YkfO4k1Q066hGXhlhDDzWYCb3kONFmOroluTz035B0rnu0F76aulrMWICTgVv3coP2_j6_GFgs0W24Xybr56zfIRUqBiJYNfupVNBh4f1m3s57MTo1A--
12, 22 -- Received: from [152.132.10.194] by web46115.mail.sp1.yahoo.com via HTTP; Tue, 07 Jul 2009 11:11:27 PDT
12, 22 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
12, 22 -- Date: Tue, 7 Jul 2009 11:11:27 -0700 (PDT)
12, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 22 -- Subject: Re: [Microscopy] Re: price for new Transmission electron microscope
12, 22 -- To: velliyka-at-umdnj.edu, MSA BB {Microscopy-at-Microscopy.Com}
12, 22 -- MIME-Version: 1.0
12, 22 -- Content-Type: text/plain; charset=iso-8859-1
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67IBTuC011368
==============================End of - Headers==============================




From: ZZhang-at-uwyo.edu
Date: Tue, 7 Jul 2009 13:33:20 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I doubt if $150K will buy a brand-new TEM, but it should be enough for same
instrument couple of years old if one is available.

Contact TEM manufacturers, particularly their service departments
and ask for a used or demo instrument of recent or current model.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com


----- Original Message -----
X-from: {velliyka-at-umdnj.edu}
To: {vitalylazar-at-att.net}
Sent: Tuesday, July 07, 2009 1:39 PM

I recently had a quote for a new, basic, "biological" TEM for $300,000, not including the digital camera, EDS etc. $150,000 is apparently not enough. A decent digital camera could easily consume 1/3 of your $150K.


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038




-----Original Message-----
X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com]
Sent: Tuesday, July 07, 2009 12:16 PM
To: Z.J. Zhang


I think they are more expensive than $150,000 US. You could probably buy a used one in that price range.

See what the rest of the listers say. I'd be curious to know the price of a new TEM myself. It will help justify keeping my old one up a running under service contract.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.


--- On Tue, 7/7/09, velliyka-at-umdnj.edu {velliyka-at-umdnj.edu} wrote:

} From: velliyka-at-umdnj.edu {velliyka-at-umdnj.edu}
} Subject: [Microscopy] Re: price for new Transmission electron microscope
} To: vapatpxs-at-yahoo.com
} Date: Tuesday, July 7, 2009, 5:45 PM
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The
} Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi, We plan to purchase new transmission electron
} microscope and our
} budget is S 150,000 (This is for only biological samples).
} Is it
} possible for us to get one with that price range?. Please
} let us know
} if any one recently bought one. Your help is appreciated.
}
} Sincerely
} V.Kabilan
} UMDNJ-NJDS
} New Jersey-NJ
}
}
} ==============================Original
} Headers==============================
} 3, 26 -- From velliyka-at-umdnj.edu
} Tue Jul  7 12:38:01 2009
} 3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU
} [130.219.34.126])
} 3, 26 --     by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with SMTP id n67Hc0kr013985
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue, 7 Jul 2009 12:38:01 -0500
} 3, 26 -- Received: from zix03.umdnj.edu (ZixVPM
} [127.0.0.1])
} 3, 26 --     by Outbound.umdnj.edu
} (Proprietary) with ESMTP id 88BBB10B7FD
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue,  7 Jul 2009 13:38:00 -0400 (EDT)
} 3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
} 3, 26 --     by zix03.umdnj.edu
} (Proprietary) with ESMTP id AB7ED10B7C1
} 3, 26 --     for {microscopy-at-microscopy.com} ;
} Tue,  7 Jul 2009 13:37:59 -0400 (EDT)
} 3, 26 -- Received: from ([10.32.15.167])
} 3, 26 --     by imail.umdnj.edu with
} ESMTP  id 8XSJWG1.19170237;
} 3, 26 --     Tue, 07 Jul 2009 13:37:56
} -0400
} 3, 26 -- MIME-version: 1.0
} 3, 26 -- Content-transfer-encoding: 7BIT
} 3, 26 -- Content-type: text/plain; charset=US-ASCII;
} format=flowed
} 3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
} 3, 26 --  by umduwc01.umdnj.edu (Sun Java(tm) System
} Messaging Server 6.3-6.03 (built
} 3, 26 --  Mar 14 2008; 32bit)) with ESMTP id {0KMF00298AB8TP30-at-umduwc01.umdnj.edu}
} for
} 3, 26 --  Microscopy-at-microscopy.com;
} Tue, 07 Jul 2009 13:37:56 -0400 (EDT)
} 3, 26 -- Message-id: {d471a6153f3c62244cb90f9ff3d95136-at-umdnj.edu}
} 3, 26 -- To: Microscopy-at-microscopy.com
} 3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
} 3, 26 -- Subject: Re:  price for new Transmission
} electron microscope
} 3, 26 -- Date: Tue, 07 Jul 2009 13:37:56 -0400
} 3, 26 -- X-Mailer: Apple Mail (2.624)
} ==============================End of -
} Headers==============================
}





==============================Original Headers==============================
12, 22 -- From vapatpxs-at-yahoo.com Tue Jul 7 13:11:32 2009
12, 22 -- Received: from web46115.mail.sp1.yahoo.com (web46115.mail.sp1.yahoo.com [68.180.199.132])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n67IBTuC011368
12, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 7 Jul 2009 13:11:30 -0500
12, 22 -- Received: (qmail 94472 invoked by uid 60001); 7 Jul 2009 18:11:27 -0000
12, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1246990287; bh=NEnlL7bPvzlGPzPGImDCpqt7LaiZ6Yw3NcLJqLk3lvg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=OjzAC+Gga5FnHCiZsEGkEpPg36/VmgDbsA55ijGwynL09YN+mRA+qhrsowCsTD/Ka+yF1b4LoSjXqkfKkKi0WKSznmVrDEKcnNXZszYuIK2M1IR6X8R2d659cyiWhCyN7o2Ns8EL++iEhiENuQCHMJirXdgn6CDG4mZpq7YHAt0=
12, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 22 -- s=s1024; d=yahoo.com;
12, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
12, 22 -- b=3lr1C/tDUzzQPxkwqmU5YZ2QFDZSOltPXM+jzCyS7vzgnHX2+oA8OtuOlT7d//KhS8N9Qeuzr5WAu+eMZ295w0fFuSiLoqYtg8j4FmT78FvHOVFGvYrKthWVwm2+06OfWkYMhIT9zBKeGbPOQdzF/TL7rR9oup68x/rdRPRBAAA=;
12, 22 -- Message-ID: {402715.92647.qm-at-web46115.mail.sp1.yahoo.com}
12, 22 -- X-YMail-OSG: VraqIowVM1kh2qMZ7aakmafDLH8sP4mw7ah6tVhDu9iKbOEKTekel3pG41xL8dr2rPoeO.dLBctJPS8iFFdQDMy65ofobq7LDkjMJowqYDQx8U2jRij3fq_Jy6KwMZj1g3.AgOGsrkZT3kCIVGKXoMQkVT0HxbdzCNjT7.fLQI8DSLdlqZl2xba9oWNRqMVBN94VYQ7H7eZPrhAzyVI7ue2YkfO4k1Q066hGXhlhDDzWYCb3kONFmOroluTz035B0rnu0F76aulrMWICTgVv3coP2_j6_GFgs0W24Xybr56zfIRUqBiJYNfupVNBh4f1m3s57MTo1A--
12, 22 -- Received: from [152.132.10.194] by web46115.mail.sp1.yahoo.com via HTTP; Tue, 07 Jul 2009 11:11:27 PDT
12, 22 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
12, 22 -- Date: Tue, 7 Jul 2009 11:11:27 -0700 (PDT)
12, 22 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 22 -- Subject: Re: [Microscopy] Re: price for new Transmission electron microscope
12, 22 -- To: velliyka-at-umdnj.edu, MSA BB {Microscopy-at-Microscopy.Com}
12, 22 -- MIME-Version: 1.0
12, 22 -- Content-Type: text/plain; charset=iso-8859-1
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67IBTuC011368
==============================End of - Headers==============================


==============================Original Headers==============================
24, 29 -- From ZZhang-at-uwyo.edu Tue Jul 7 13:33:20 2009
24, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67IXKRg007559
24, 29 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:33:20 -0500
24, 29 -- Received: from ponyexpress-ht2.uwyo.edu (ponyexpress-ht2.uwyo.edu [10.84.60.209])
24, 29 -- by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n67IXHwN001450
24, 29 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL)
24, 29 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 12:33:17 -0600 (MDT)
24, 29 -- (envelope-from ZZhang-at-uwyo.edu)
24, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht2
24, 29 -- ([10.84.60.209]) with mapi; Tue, 7 Jul 2009 12:33:18 -0600
24, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
24, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
24, 29 -- Date: Tue, 7 Jul 2009 12:33:16 -0600
24, 29 -- Subject: RE: price for new Transmission electron microscope
24, 29 -- Thread-Topic: price for new Transmission electron microscope
24, 29 -- Thread-Index: Acn/LwKmW4DVHv/yTS6O1aMrtZeKqAAAREfA
24, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE33FC831C-at-ponyexpress-mb2}
24, 29 -- References: {200907071816.n67IG4LH023782-at-ns.microscopy.com}
24, 29 -- In-Reply-To: {200907071816.n67IG4LH023782-at-ns.microscopy.com}
24, 29 -- Accept-Language: en-US
24, 29 -- Content-Language: en-US
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- acceptlanguage: en-US
24, 29 -- Content-Type: text/plain; charset="iso-8859-1"
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67IXKRg007559
==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Tue, 7 Jul 2009 13:35:21 -0500
Subject: [Microscopy] Re: price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've responded via personal message, but for the benefit of the listers, here's my experience:

New TEMs are more automated than ever, keeping the purchase price over $400,000. The cheapest EM digital camera quote I could get to upgrade our old TEM to digital was $42,000 for just the camera system.

Two routes appear to be available for used EMs:
1) Auction houses: When industry closes a research or production site, the assets are often put in the hands of online auction houses specializing in business equipment only. (E-bay for business equipment) Remember that you will be charged a premium over your bid (~17% in some cases). Also remember that moving and setting up an EM can cost over $16,000. Also note that EMs don't like to be shut down for long periods of time. Old parts that were hanging on will be prone to malfunction. Keep a repair budget on hand for that first year. There may also be parts missing from the equipment, as packing and moving isn't always performed by happy workers, some of which are just filling time until the official layoff notice comes.

2) Refurbished scopes removed from a site in favor of newer models can be had, but are often spoken for before they are even removed. I've been told that the waiting list is over a year long, and unpredictable.

Good luck,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
X-from: velliyka-at-umdnj.edu [mailto:velliyka-at-umdnj.edu]
Sent: Tuesday, July 07, 2009 1:46 PM
To: Sobocinski, Gregg

Hi, We plan to purchase new transmission electron microscope and our
budget is S 150,000 (This is for only biological samples). Is it
possible for us to get one with that price range?. Please let us know
if any one recently bought one. Your help is appreciated.

Sincerely
V.Kabilan
UMDNJ-NJDS
New Jersey-NJ


==============================Original Headers==============================
3, 26 -- From velliyka-at-umdnj.edu Tue Jul 7 12:38:01 2009
3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n67Hc0kr013985
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 12:38:01 -0500
3, 26 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 88BBB10B7FD
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:38:00 -0400 (EDT)
3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
3, 26 -- by zix03.umdnj.edu (Proprietary) with ESMTP id AB7ED10B7C1
3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:37:59 -0400 (EDT)
3, 26 -- Received: from ([10.32.15.167])
3, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.19170237;
3, 26 -- Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- MIME-version: 1.0
3, 26 -- Content-transfer-encoding: 7BIT
3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
3, 26 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
3, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KMF00298AB8TP30-at-umduwc01.umdnj.edu} for
3, 26 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 13:37:56 -0400 (EDT)
3, 26 -- Message-id: {d471a6153f3c62244cb90f9ff3d95136-at-umdnj.edu}
3, 26 -- To: Microscopy-at-microscopy.com
3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
3, 26 -- Subject: Re: price for new Transmission electron microscope
3, 26 -- Date: Tue, 07 Jul 2009 13:37:56 -0400
3, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================


==============================Original Headers==============================
15, 25 -- From greggps-at-umich.edu Tue Jul 7 13:35:20 2009
15, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67IZKEF011344
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 13:35:20 -0500
15, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.235]) by
15, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Tue, 7 Jul
15, 25 -- 2009 14:35:19 -0400
15, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu}
15, 25 -- To: Microscopy MSA {Microscopy-at-microscopy.com}
15, 25 -- Date: Tue, 7 Jul 2009 14:35:17 -0400
15, 25 -- Subject: RE: [Microscopy] Re: price for new Transmission electron microscope
15, 25 -- Thread-Topic: [Microscopy] Re: price for new Transmission electron microscope
15, 25 -- Thread-Index: Acn/Ksegb3DFW9gkTkqreOEyzCSTtAABM4JQ
15, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA39340615A1842D-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu}
15, 25 -- References: {200907071745.n67HjeK9025883-at-ns.microscopy.com}
15, 25 -- In-Reply-To: {200907071745.n67HjeK9025883-at-ns.microscopy.com}
15, 25 -- Accept-Language: en-US
15, 25 -- Content-Language: en-US
15, 25 -- X-MS-Has-Attach:
15, 25 -- X-MS-TNEF-Correlator:
15, 25 -- acceptlanguage: en-US
15, 25 -- Content-Type: text/plain; charset="us-ascii"
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67IZKEF011344
==============================End of - Headers==============================




From: msa-at-lv-em.com
Date: Tue, 7 Jul 2009 14:02:46 -0500
Subject: [Microscopy] RE: price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All.

After seeing a few of the replies to this posting, I wanted to put our
system pricing out there. Usually, a vendor can't post this information on
the list, but given the specific topic being discussed I think it is
relevant and appropriate.

Our TEM is a FEG (Schottky) that operates in vacuum and has 2 nm resolution.

It is a benchtop system.

It has optional Scanning modes: SEM (3 nm. resolution) and STEM (2 nm.
resolution).

The TEM system price is 135,000 (SEM mode add 10,000, STEM add another
10,000).
Add an additional 10,000 for our high end (2K x 2K cooled) CCD digital
camera.

So for 145,000 you can get a fully functional TEM with 2K digital camera
(and there are no hidden charges...this price includes all components plus
installation, training and 1 year service).

The LVEM5 does NOT do everything that EVERY other EM does (no equipment does
everything and EM is no different) so you may want to visit our website to
learn more. Or contact me directly.

www.lv-em.com


see you at M&M (booth 741)


Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.

Montreal, Canada

www.lv-em.com




==============================Original Headers==============================
21, 19 -- From msa-at-lv-em.com Tue Jul 7 14:02:45 2009
21, 19 -- Received: from relais.videotron.ca (relais.videotron.ca [24.201.245.36])
21, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67J2jIJ004156
21, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:02:45 -0500
21, 19 -- MIME-version: 1.0
21, 19 -- Content-transfer-encoding: 7BIT
21, 19 -- Content-type: text/plain; charset=US-ASCII
21, 19 -- Received: from DF92QRH1 ([74.56.174.139]) by VL-MO-MR004.ip.videotron.ca
21, 19 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
21, 19 -- with ESMTP id {0KMF00728E88DGF0-at-VL-MO-MR004.ip.videotron.ca} for
21, 19 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:02:32 -0400 (EDT)
21, 19 -- From: msa-at-lv-em.com
21, 19 -- To: Microscopy-at-microscopy.com
21, 19 -- Subject: RE: [Microscopy] price for new Transmission electron microscope
21, 19 -- Date: Tue, 07 Jul 2009 15:02:35 -0400
21, 19 -- Message-id: {006401c9ff35$7d94e7b0$78beb710$-at-com}
21, 19 -- X-Mailer: Microsoft Office Outlook 12.0
21, 19 -- Thread-index: Acn/L00r3RRA4S6kSqiZoRJqKeiP3QAATc9wAAD1OnA=
21, 19 -- Content-language: en-us
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 7 Jul 2009 14:27:25 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25 -0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 -- id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
8, 24 -- (PMDF V6.3-x18 #31556) with ESMTPA id {01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE: price for new Transmission electron microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================




From: msa-at-lv-em.com
Date: Tue, 7 Jul 2009 14:41:31 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 -- id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
er6s1.ecr6.ohio-state.edu
8, 24 -- (PMDF V6.3-x18 #31556) with ESMTPA id
{01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE: price for new Transmission electron
microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul 7 14:41:31 2009
24, 21 -- Received: from relais.videotron.ca (relais.videotron.ca [24.201.245.36])
24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67JfVs8000863
24, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT
24, 21 -- Content-type: text/plain; charset=US-ASCII
24, 21 -- Received: from DF92QRH1 ([74.56.174.139]) by VL-MH-MR002.ip.videotron.ca
24, 21 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
24, 21 -- with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca} for
24, 21 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30 -0400 (EDT)
24, 21 -- From: msa-at-lv-em.com
24, 21 -- To: Microscopy-at-microscopy.com
24, 21 -- References: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy] price for new Transmission electron microscope
24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400
24, 21 -- Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0
24, 21 -- Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of - Headers==============================




From: tom-at-TomKaye.com
Date: Tue, 7 Jul 2009 14:59:59 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

Searching the net looking for info on permanent magnet SEM's I came across
this bit of trivia. A full brochure of RCA's 1950's tabletop SEM. I don't
think I have seen this on the listserv before so here is the link.

http://www.smecc.org/rca_emt_tabletop.htm

Tom Kaye





-----Original Message-----
X-from: msa-at-lv-em.com [mailto:msa-at-lv-em.com]
Sent: Tuesday, July 07, 2009 12:44 PM
To: tom-at-tomkaye.com

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 -- id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
er6s1.ecr6.ohio-state.edu
8, 24 -- (PMDF V6.3-x18 #31556) with ESMTPA id
{01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE: price for new Transmission electron
microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul 7 14:41:31 2009
24, 21 -- Received: from relais.videotron.ca (relais.videotron.ca
[24.201.245.36])
24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JfVs8000863
24, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT
24, 21 -- Content-type: text/plain; charset=US-ASCII
24, 21 -- Received: from DF92QRH1 ([74.56.174.139]) by
VL-MH-MR002.ip.videotron.ca
24, 21 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3
2007; 32bit))
24, 21 -- with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca} for
24, 21 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30 -0400 (EDT)
24, 21 -- From: msa-at-lv-em.com
24, 21 -- To: Microscopy-at-microscopy.com
24, 21 -- References: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy] price for new Transmission electron
microscope
24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400
24, 21 -- Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0
24, 21 -- Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of - Headers==============================


==============================Original Headers==============================
39, 22 -- From tom-at-TomKaye.com Tue Jul 7 14:59:58 2009
39, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com [66.102.127.13])
39, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67JxwUY015667
39, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:59:58 -0500
39, 22 -- Received: from TKLAPTOP ([216.75.124.10]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959);
39, 22 -- Tue, 7 Jul 2009 14:59:57 -0500
39, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com}
39, 22 -- To: {microscopy-at-microscopy.com}
39, 22 -- Subject: Permanent Magnet Tabletop SEM---1950 Historical Note
39, 22 -- Date: Tue, 7 Jul 2009 13:00:00 -0700
39, 22 -- Message-ID: {LHECKJAKKIOGKDBHHJMLCEJJDGAA.tom-at-tomkaye.com}
39, 22 -- MIME-Version: 1.0
39, 22 -- Content-Type: text/plain;
39, 22 -- charset="iso-8859-1"
39, 22 -- Content-Transfer-Encoding: 7bit
39, 22 -- X-Priority: 3 (Normal)
39, 22 -- X-MSMail-Priority: Normal
39, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0)
39, 22 -- In-Reply-To: {200907071944.n67JiOCQ006854-at-ns.microscopy.com}
39, 22 -- Importance: Normal
39, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
39, 22 -- X-OriginalArrivalTime: 07 Jul 2009 19:59:57.0639 (UTC) FILETIME=[80D65D70:01C9FF3D]
==============================End of - Headers==============================




From: ZZhang-at-uwyo.edu
Date: Tue, 7 Jul 2009 15:06:19 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For a 5 kV tabletop SEM (such as the Hitachi TM 1000), the resolution is about 30 nm.

I am curious how the LV-EM5 reaches its 2-3 nm resolution. I am not saying it cannot, but it seems to me that everything needs to be perfect in order to do so.


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038





-----Original Message-----
X-from: msa-at-lv-em.com [mailto:msa-at-lv-em.com]
Sent: Tuesday, July 07, 2009 1:45 PM
To: Z.J. Zhang

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 -- id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
er6s1.ecr6.ohio-state.edu
8, 24 -- (PMDF V6.3-x18 #31556) with ESMTPA id
{01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE: price for new Transmission electron
microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul 7 14:41:31 2009
24, 21 -- Received: from relais.videotron.ca (relais.videotron.ca [24.201.245.36])
24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67JfVs8000863
24, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT
24, 21 -- Content-type: text/plain; charset=US-ASCII
24, 21 -- Received: from DF92QRH1 ([74.56.174.139]) by VL-MH-MR002.ip.videotron.ca
24, 21 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
24, 21 -- with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca} for
24, 21 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30 -0400 (EDT)
24, 21 -- From: msa-at-lv-em.com
24, 21 -- To: Microscopy-at-microscopy.com
24, 21 -- References: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy] price for new Transmission electron microscope
24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400
24, 21 -- Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0
24, 21 -- Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of - Headers==============================


==============================Original Headers==============================
38, 30 -- From ZZhang-at-uwyo.edu Tue Jul 7 15:06:19 2009
38, 30 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
38, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67K6JSJ029429
38, 30 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 15:06:19 -0500
38, 30 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208])
38, 30 -- by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n67K68wR028290
38, 30 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL)
38, 30 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:06:17 -0600 (MDT)
38, 30 -- (envelope-from ZZhang-at-uwyo.edu)
38, 30 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht1
38, 30 -- ([10.84.60.208]) with mapi; Tue, 7 Jul 2009 14:06:12 -0600
38, 30 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
38, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
38, 30 -- Date: Tue, 7 Jul 2009 14:06:10 -0600
38, 30 -- Subject: RE: [Microscopy] RE: price for new Transmission electron microscope
38, 30 -- Thread-Topic: [Microscopy] RE: price for new Transmission electron
38, 30 -- microscope
38, 30 -- Thread-Index: Acn/O2EDlHbIZXKCQeW1kLYO1IZHxAAAMuMA
38, 30 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE33FC839D-at-ponyexpress-mb2}
38, 30 -- References: {200907071944.n67JiXFg007188-at-ns.microscopy.com}
38, 30 -- In-Reply-To: {200907071944.n67JiXFg007188-at-ns.microscopy.com}
38, 30 -- Accept-Language: en-US
38, 30 -- Content-Language: en-US
38, 30 -- X-MS-Has-Attach:
38, 30 -- X-MS-TNEF-Correlator:
38, 30 -- acceptlanguage: en-US
38, 30 -- Content-Type: text/plain; charset="us-ascii"
38, 30 -- MIME-Version: 1.0
38, 30 -- Content-Transfer-Encoding: 8bit
38, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67K6JSJ029429
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 7 Jul 2009 16:21:29 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,
There was one of these sitting in a lab at SUNY Syracuse Environmental
Science & Forestry (Baker Lab) for years. It was not operating in the late
70s and early 80s, and I would have loved to have gotten my hands on it. I
don't know what happened to it. Hope it didn't go to the dump.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: tom-at-TomKaye.com [mailto:tom-at-TomKaye.com]
Sent: Tuesday, July 07, 2009 4:02 PM
To: kenconverse-at-qualityimages.biz

All,

Searching the net looking for info on permanent magnet SEM's I came across
this bit of trivia. A full brochure of RCA's 1950's tabletop SEM. I don't
think I have seen this on the listserv before so here is the link.

http://www.smecc.org/rca_emt_tabletop.htm

Tom Kaye





-----Original Message-----
X-from: msa-at-lv-em.com [mailto:msa-at-lv-em.com]
Sent: Tuesday, July 07, 2009 12:44 PM
To: tom-at-tomkaye.com

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 -- id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
er6s1.ecr6.ohio-state.edu
8, 24 -- (PMDF V6.3-x18 #31556) with ESMTPA id
{01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE: price for new Transmission electron
microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul 7 14:41:31 2009
24, 21 -- Received: from relais.videotron.ca (relais.videotron.ca
[24.201.245.36])
24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JfVs8000863
24, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT
24, 21 -- Content-type: text/plain; charset=US-ASCII
24, 21 -- Received: from DF92QRH1 ([74.56.174.139]) by
VL-MH-MR002.ip.videotron.ca
24, 21 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3
2007; 32bit))
24, 21 -- with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca} for
24, 21 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30 -0400 (EDT)
24, 21 -- From: msa-at-lv-em.com
24, 21 -- To: Microscopy-at-microscopy.com
24, 21 -- References: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy] price for new Transmission electron
microscope
24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400
24, 21 -- Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0
24, 21 -- Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of - Headers==============================


==============================Original Headers==============================
39, 22 -- From tom-at-TomKaye.com Tue Jul 7 14:59:58 2009
39, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com
[66.102.127.13])
39, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JxwUY015667
39, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:59:58
-0500
39, 22 -- Received: from TKLAPTOP ([216.75.124.10]) by
w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959);
39, 22 -- Tue, 7 Jul 2009 14:59:57 -0500
39, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com}
39, 22 -- To: {microscopy-at-microscopy.com}
39, 22 -- Subject: Permanent Magnet Tabletop SEM---1950 Historical Note
39, 22 -- Date: Tue, 7 Jul 2009 13:00:00 -0700
39, 22 -- Message-ID: {LHECKJAKKIOGKDBHHJMLCEJJDGAA.tom-at-tomkaye.com}
39, 22 -- MIME-Version: 1.0
39, 22 -- Content-Type: text/plain;
39, 22 -- charset="iso-8859-1"
39, 22 -- Content-Transfer-Encoding: 7bit
39, 22 -- X-Priority: 3 (Normal)
39, 22 -- X-MSMail-Priority: Normal
39, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0)
39, 22 -- In-Reply-To: {200907071944.n67JiOCQ006854-at-ns.microscopy.com}
39, 22 -- Importance: Normal
39, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
39, 22 -- X-OriginalArrivalTime: 07 Jul 2009 19:59:57.0639 (UTC)
FILETIME=[80D65D70:01C9FF3D]
==============================End of - Headers==============================




==============================Original Headers==============================
51, 25 -- From kenconverse-at-qualityimages.biz Tue Jul 7 16:21:29 2009
51, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
51, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67LLSS8014819
51, 25 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 16:21:29 -0500
51, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
51, 25 -- with ESMTP
51, 25 -- id {20090707212128007.OLCM19903-at-cdptpa-omta03.mail.rr.com} ;
51, 25 -- Tue, 7 Jul 2009 21:21:28 +0000
51, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
51, 25 -- To: {tom-at-TomKaye.com} , "MSA Listserver" {microscopy-at-microscopy.com}
51, 25 -- Subject: RE: [Microscopy] Permanent Magnet Tabletop SEM---1950 Historical Note
51, 25 -- Date: Tue, 7 Jul 2009 17:21:19 -0400
51, 25 -- Message-ID: {663A850846594C87AA24A1386168796F-at-Ken}
51, 25 -- MIME-Version: 1.0
51, 25 -- Content-Type: text/plain;
51, 25 -- charset="us-ascii"
51, 25 -- X-Priority: 3 (Normal)
51, 25 -- X-MSMail-Priority: Normal
51, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
51, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
51, 25 -- Thread-Index: Acn/PcpdInKo+NGsQcO9YGgDrz/yEwACoDvQ
51, 25 -- Importance: Normal
51, 25 -- In-Reply-To: {200907072001.n67K1pmr019583-at-ns.microscopy.com}
51, 25 -- Content-Transfer-Encoding: 8bit
51, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67LLSS8014819
==============================End of - Headers==============================




From: cwinkler-at-drexel.edu
Date: Tue, 7 Jul 2009 21:08:04 -0500
Subject: [Microscopy] viaWWW: Tips or techniques for imaging magnetic materials in the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dale,

Yes your right TEM, my bad.

Tom



-----Original Message-----
X-from: Dale Callaham [mailto:dac-at-research.umass.edu]
Sent: Tuesday, July 07, 2009 1:14 PM
To: tom-at-TomKaye.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cwinkler-at-drexel.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cwinkler-at-drexel.edu
Name: Christopher Winkler

Organization: Drexel University

Title-Subject: [Filtered] Tips or techniques for imaging magnetic
materials in the TEM?

Question: Hello all,

One of the students in our research group needs to examine the
microstructure of low alloyed soft magnetic steels via TEM.
Preliminary work with these steels has left me frustrated, as the
interaction between the magnetism present in the steels and the
magnetic field in the objective lens causes two major headaches: one,
the astigmatism in the objective and condenser lenses changes as I
translate around the plane of the sample and two, the beam is
strongly deflected when I tilt in both the x and y directions. The
former problem can be ameliorated by using the caustic spot to
correct for C-stig and O-stig (see
doi:10.1016/j.ultramic.2008.11.014) but I don't see a way to mitigate
the strong beam deflection that results from tilting the sample. Can
anyone offer suggestions on how to minimize or eliminate this beam
deflection?

Thank you.

Login Host: 129.25.18.137
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Tue Jul 7 21:08:04 2009
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68283KB021824
8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 21:08:04 -0500
8, 12 -- Mime-Version: 1.0
8, 12 -- Message-Id: {p06240802c679aff01b61-at-[206.69.208.22]}
8, 12 -- Date: Tue, 7 Jul 2009 21:08:03 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: cwinkler-at-drexel.edu (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Tips or techniques for imaging magnetic materials in the
8, 12 -- TEM?
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 7 Jul 2009 22:41:58 -0500
Subject: [Microscopy] Re: viaWWW: Tips or techniques for imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Welcome to the world of Zeiss electrostatic
final lenses versus other's magnetic immersion lenses.

Older systems may not have this issue. But
magnetic final lenses are a big issue. Do as you will.

Good luck.

gary g.


At 07:09 PM 7/7/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 21 -- From gary-at-gaugler.com Tue Jul 7 22:41:58 2009
10, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n683fv0k006425
10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 22:41:58 -0500
10, 21 -- Message-Id: {200907080341.n683fv0k006425-at-ns.microscopy.com}
10, 21 -- Received: (qmail 3975 invoked from network); 7 Jul 2009 20:31:27 -0700
10, 21 -- Received: by simscan 1.1.0 ppid: 3972, pid: 3973, t: 0.1061s
10, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 21 -- by smtp2 with SMTP; 7 Jul 2009 20:31:27 -0700
10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 21 -- Date: Tue, 07 Jul 2009 20:41:46 -0700
10, 21 -- To: cwinkler-at-drexel.edu
10, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 21 -- Subject: Re: [Microscopy] viaWWW: Tips or techniques for imaging
10, 21 -- magnetic materials in the
10, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 21 -- In-Reply-To: {200907080209.n6829fwr024220-at-ns.microscopy.com}
10, 21 -- References: {200907080209.n6829fwr024220-at-ns.microscopy.com}
10, 21 -- Mime-Version: 1.0
10, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jean-paul.bailon-at-polymtl.ca
Date: Tue, 7 Jul 2009 23:36:46 -0500
Subject: [Microscopy] Re: Sem stereo images question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Nikky !

It is quite easy to get stunning stereo pictures in a SEM. Following is my
receipt to get excellent stereo photomicrograph.

1) First of all, be sure that your specimen holder is oriented (playing on the
rotation) in such a way that when you will tilt your specimen, this tilting
will be along a vertical line (North-South direction).

2) Take a first picture of the area of interest.

3) Take care to carefully note the exact position of some points of interest in
this first picture (particularly their vertical positions = Y coordinate). If
your SEM is equipped with a conventional CRT screen, you can use an erasable
marker to note the position of some remarkable details of this first photo
directly on the screen. With the new digital generation of SEM equipped with
LCD monitor, most of their software will allow you to use the “ruler” or
“marker” command to clearly pinpoint two remarkable details on this first
photo. Or put an acetate sheet on the LCD screen and use an erasable marker on
this sheet.

4) Tilt your specimen in the clockwise direction by 7, 8 or 9 degrees. When
doing that, the area of interest will be shifted. So, go to 8 degrees (for
example) by small steps when keeping your area of interest in view by playing
on the X and Y positions of the sample holder at each step.

5) Refocus your area of interest. The best way is to take a detail in the center
of this area and to focus it by playing on the Z position of the specimen. If
you are working at small magnifications (e.g. { 2 K) and with a large depth of
field (high working distance WD associated with a small objective aperture),
you can refocus the area of interest with the “Focus” knob of the SEM; it is
easier than to refocus with the Z position of the specimen.

6) By playing with the X and Y positions of the area of interest, be sure that
the remarkable details already pinpointed at the step 3 are now located at the
same positions that they have in the first picture (taken at step 2). Their
vertical position is particularly important. Most generally, their horizontal
distance will have changed. So, keep their new horizontal position in between
the previous one.

7) Take the second picture of your area of interest.

Et VOILA! You are done! Now, to view your area of interest in stereo, you need
a stereo viewer under which you place your two views (left and right) and play
on their positions up until you get a stereo image.

You can also prepare an anaglyph with these two photos, the first one being
tinted in red and the second one tinted in blue (or green). To do that, I
suggest you to download the freeware ANAMAKER
(http://www.stereoeye.jp/software/index_e.html).

It is easy to prepare an anaglyph with this program which allows correcting some
X and Y misalignments between the two photos. The anaglyph generated by Anamaker
can now be viewed through Red-Blue (or Red-Green) glasses.

Good lucks and enjoy the stereo world.

Jean-Paul Bailon


} Greetings all
} I have a student who wishes to do some stereo images, which I have no
} experience of. Could you tell me the best software to use to merge the 2
} tilted images. Or some good references.
}
} Many thanks
} Nikki

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Prof. Jean-Paul Baïlon jean-paul.bailon-at-polymtl.ca
Dépt. de Génie mécanique Tél.: 1(514) 340 4711, p. 4260
École Polytechnique Fax.: 1(514) 340 4468
CP6079, Succ. Centre-Ville
Montréal (QC) Canada H3C 3A7
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

==============================Original Headers==============================
17, 21 -- From jean-paul.bailon-at-polymtl.ca Tue Jul 7 23:36:45 2009
17, 21 -- Received: from smtp.polymtl.ca (smtp.polymtl.ca [132.207.4.11])
17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n684ajuN022781
17, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 23:36:45 -0500
17, 21 -- Received: from localhost (imp-4-2.polymtl.ca [132.207.4.77])
17, 21 -- by smtp.polymtl.ca (8.14.3/8.14.3) with ESMTP id n684aiZM000887
17, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 00:36:44 -0400
17, 21 -- Received: from bas8-montreal28-1242355051.dsl.bell.ca (bas8-montreal28-1242355051.dsl.bell.ca [74.12.213.107])
17, 21 -- by www.imp.polymtl.ca (IMP) with HTTP
17, 21 -- for {p500203-at-132.207.4.131} ; Wed, 08 Jul 2009 00:36:44 -0400
17, 21 -- Message-ID: {1247027804.4a54225c192b5-at-www.imp.polymtl.ca}
17, 21 -- Date: Wed, 08 Jul 2009 00:36:44 -0400
17, 21 -- From: Jean-Paul =?iso-8859-1?b?QmHvbG9u?= {jean-paul.bailon-at-polymtl.ca}
17, 21 -- To: Microscopy-at-microscopy.com
17, 21 -- Subject: Re: Sem stereo images question
17, 21 -- MIME-Version: 1.0
17, 21 -- Content-Type: text/plain; charset=ISO-8859-1
17, 21 -- Content-Transfer-Encoding: 8bit
17, 21 -- User-Agent: Internet Messaging Program (IMP) 3.2.3
17, 21 -- X-Originating-IP: 74.12.213.107
17, 21 -- X-Poly-FromMTA: (imp-4-2.polymtl.ca [132.207.4.77]) at Wed, 8 Jul 2009 04:36:44 +0000
==============================End of - Headers==============================




From: ron.doole-at-materials.ox.ac.uk
Date: Wed, 8 Jul 2009 00:04:19 -0500
Subject: [Microscopy] Re: viaWWW: Tips or techniques for imaging magnetic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Christopher,

Yes, TEM of magnetic materials will always be a problem but there are ways in
which you can reduce the various effects.

First - and I hope it is not too late, NEVER put a sample in a holder that does
not have a positive clamping system. Always use a screwed specimen clamp not a
friction held one (those old wire circlips are not good). This is to prevent
the high field of the objective lens ripping your sample out of the holder. If
you are using a tilting holder make sure the gimbal is also positively held and
not a frction drive (eg the old JEOL 100/200 style) otherwise your sample may
just align with the vertical lens field. I would always switch the obj lens to
a low setting (using the focus knob) when inserting or removing the sample and
then increase the current to focus when the sample is in the column. This tends
to avoid the sample coming out of the holder on the way into the column. If you
do lose your sample in the column don't worry you (or the service engineer)
will find it standing vertically on the pole piece. It will probably be
preventing anyone from using the instrument.

Second - reduce the bulk of the magnetic material as much as possible. How are
you making your samples? If you are using a 3mm disc for electropolishing or
ion milling it needs to be ground as thin as possible (20um?) before final
polishing so that the rim is also thin. If you are using a 'tenupol' style
electropolisher you will need to pack Pt washers either side of your sample to
ensure that you get contact and no electrolyte seepage around the edges as the
sample needs to be too thin for most holders. Can you get a FIB sample welded
onto a TEM grid? That is probably the least amount of material you can get.

Third - try to keep the hole central and not too big. The ideal sample would
not have a hole just a thin area in the centre. Think of it on the microscope's
scale. You have an electron beam that will be affected by a magnetic field with
a magnet (sample) one side of the beam and not the other, you are bound to get
problems. If you can reduce the difference in magnetic field between the two
sides of the beam you will improve your situation. Don't look at the very edge
of the sample look further in so that you have sample all around the beam, if
the sample is not thin enough to look further in than the very edge then you
will need to make better samples.

Other hints.
Don't try to set the eucentric height by rocking the sample, it just won't
work. Set the eucentric height by setting the correct objective lens current
and focus by adjusting the sample height. If you don't know the focus current
set up a non magnetic (eg C film) sample first.
Don't bother too much with the beam tilt and obj astigmatism until you have set
up your sample area and tilt, you will need to reset them whenever you change
area.
Use the highest accelerating voltage available to you, the more energetic the
electron beam the harder it is to deflect.

Finally - remember if it was easy some else would do the work!

Regards,
Ron


In message {200907080218.n682IJKV003642-at-ns.microscopy.com} cwinkler-at-drexel.edu
writes:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both cwinkler-at-drexel.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: cwinkler-at-drexel.edu
} Name: Christopher Winkler
}
} Organization: Drexel University
}
} Title-Subject: [Filtered] Tips or techniques for imaging magnetic
} materials in the TEM?
}
} Question: Hello all,
}
} One of the students in our research group needs to examine the
} microstructure of low alloyed soft magnetic steels via TEM.
} Preliminary work with these steels has left me frustrated, as the
} interaction between the magnetism present in the steels and the
} magnetic field in the objective lens causes two major headaches: one,
} the astigmatism in the objective and condenser lenses changes as I
} translate around the plane of the sample and two, the beam is
} strongly deflected when I tilt in both the x and y directions. The
} former problem can be ameliorated by using the caustic spot to
} correct for C-stig and O-stig (see
} doi:10.1016/j.ultramic.2008.11.014) but I don't see a way to mitigate
} the strong beam deflection that results from tilting the sample. Can
} anyone offer suggestions on how to minimize or eliminate this beam
} deflection?
}
} Thank you.
}
} Login Host: 129.25.18.137
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 7 21:08:04 2009
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n68283KB021824
} 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 21:08:04 -0500
} 8, 12 -- Mime-Version: 1.0
} 8, 12 -- Message-Id: {p06240802c679aff01b61-at-[206.69.208.22]}
} 8, 12 -- Date: Tue, 7 Jul 2009 21:08:03 -0500
} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: cwinkler-at-drexel.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: Tips or techniques for imaging magnetic materials
in the
} 8, 12 -- TEM?
} 8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================

--
Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk

==============================Original Headers==============================
11, 25 -- From ron.doole-at-materials.ox.ac.uk Wed Jul 8 00:04:18 2009
11, 25 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169])
11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6854I78005826
11, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 00:04:18 -0500
11, 25 -- Received: from webmail222.herald.ox.ac.uk ([163.1.0.222])
11, 25 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69)
11, 25 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
11, 25 -- id 1MOPKJ-0006W1-UB; Wed, 08 Jul 2009 06:04:15 +0100
11, 25 -- Received: by webmail222.herald.ox.ac.uk (Postfix, from userid 33)
11, 25 -- id EE4A11400C; Wed, 8 Jul 2009 06:04:14 +0100 (BST)
11, 25 -- Content-Type: text/plain
11, 25 -- Content-Disposition: inline
11, 25 -- Content-Transfer-Encoding: 7bit
11, 25 -- MIME-Version: 1.0
11, 25 -- X-Mailer: MIME-tools 5.420 (Entity 5.420)
11, 25 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
11, 25 -- Date: Wed, 08 Jul 2009 06:04:14 +0100
11, 25 -- To: cwinkler-at-drexel.edu
11, 25 -- CC: Microscopy-at-microscopy.com
11, 25 -- Subject: Re: [Microscopy] viaWWW: Tips or techniques for imaging magnetic
11, 25 -- materials in the
11, 25 -- In-Reply-To: {200907080218.n682IJKV003642-at-ns.microscopy.com}
11, 25 -- X-Webmail-Sender: rdoole
11, 25 -- X-Webmail-Originating-Ip: 86.157.38.71
11, 25 -- Message-Id: {20090708050414.EE4A11400C-at-webmail222.herald.ox.ac.uk}
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 8 Jul 2009 02:46:05 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have no interest in this microscope, not even as a user. But I am must say that microscope IS unique.
I think that this alternative is interesting however Ephram should have been a little bit more objective with the features of this instrument as this list is not used for advertisement but instead for information purposes. In this case one should better list the pluses AND minuses of the instrument (and not list the pluses and give a link to discover the minuses by yourself).
Now the original post stated that it would be used for applications in biology.
Fact is that the contrasting mechanism is really special and very interesting for biological samples BUT on the minus side one has to cut very very thin sections, no more than 50nm (remember: 5kV). Objectively it is not something easy to do, I simply couldn't do it. I wonder what colour have sections of this thickness floating on water but I suppose they are not brightly coloured :-).
This information is not especially easy to find on the website (look in product-specimen preparation) although it is a critical inconvenience I think.

To complement the other posts about the purchase of a TEM instrument: please do not underestimate the running costs of the instrument and the costs of sample preparation.

Best regards,

Stephane 




----- Original Message ----
X-from: "ZZhang-at-uwyo.edu" {ZZhang-at-uwyo.edu}
To: nizets2-at-yahoo.com
Sent: Tuesday, July 7, 2009 10:09:32 PM

For a 5 kV tabletop SEM (such as the Hitachi TM 1000), the resolution is about 30 nm.

I am curious how the LV-EM5 reaches its 2-3 nm resolution. I am not saying it cannot, but it seems to me that everything needs to be perfect in order to do so.


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038





-----Original Message-----
X-from: msa-at-lv-em.com [mailto:msa-at-lv-em.com]
Sent: Tuesday, July 07, 2009 1:45 PM
To: Z.J. Zhang

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it
is a 5kV permanent magnet electron microscope with whatever pluses
and minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

Hendrik O. Colijn                      colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674                  http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul  7 14:27:25 2009
8, 24 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n67JRO2B018834
8, 24 --     for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 --  er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 24 --  id {01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for
Microscopy-at-microscopy.com;
8, 24 --  Tue, 07 Jul 2009 15:27:18 -0400 (EDT)
8, 24 -- Received: from HOC1.ecr6.ohio-state.edu
8, 24 --  (hoc1.ceof.ohio-state.edu [164.107.76.179]) by
er6s1.ecr6.ohio-state.edu
8, 24 --  (PMDF V6.3-x18 #31556) with ESMTPA id
{01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 --  for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400
8, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 24 -- Subject: Re: [Microscopy] RE:  price for new Transmission electron
microscope
8, 24 -- In-reply-to: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 24 -- To: Microscopy-at-microscopy.com
8, 24 -- Message-id: {01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 24 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 24 -- References: {200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of - Headers==============================


==============================Original Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul  7 14:41:31 2009
24, 21 -- Received: from relais.videotron.ca (relais.videotron.ca [24.201.245.36])
24, 21 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67JfVs8000863
24, 21 --     for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT
24, 21 -- Content-type: text/plain; charset=US-ASCII
24, 21 -- Received: from DF92QRH1 ([74.56.174.139]) by VL-MH-MR002.ip.videotron.ca
24, 21 --  (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug  3 2007; 32bit))
24, 21 --  with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca} for
24, 21 --  Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30 -0400 (EDT)
24, 21 -- From: msa-at-lv-em.com
24, 21 -- To: Microscopy-at-microscopy.com
24, 21 -- References: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy]  price for new Transmission electron microscope
24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400
24, 21 -- Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0
24, 21 -- Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of - Headers==============================


==============================Original Headers==============================
38, 30 -- From ZZhang-at-uwyo.edu Tue Jul  7 15:06:19 2009
38, 30 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
38, 30 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n67K6JSJ029429
38, 30 --     for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 15:06:19 -0500
38, 30 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208])
38, 30 --     by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n67K68wR028290
38, 30 --     (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL)
38, 30 --     for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009 14:06:17 -0600 (MDT)
38, 30 --     (envelope-from ZZhang-at-uwyo.edu)
38, 30 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht1
38, 30 --  ([10.84.60.208]) with mapi; Tue, 7 Jul 2009 14:06:12 -0600
38, 30 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
38, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
38, 30 -- Date: Tue, 7 Jul 2009 14:06:10 -0600
38, 30 -- Subject: RE: [Microscopy] RE:  price for new Transmission electron microscope
38, 30 -- Thread-Topic: [Microscopy] RE:  price for new Transmission electron
38, 30 --  microscope
38, 30 -- Thread-Index: Acn/O2EDlHbIZXKCQeW1kLYO1IZHxAAAMuMA
38, 30 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE33FC839D-at-ponyexpress-mb2}
38, 30 -- References: {200907071944.n67JiXFg007188-at-ns.microscopy.com}
38, 30 -- In-Reply-To: {200907071944.n67JiXFg007188-at-ns.microscopy.com}
38, 30 -- Accept-Language: en-US
38, 30 -- Content-Language: en-US
38, 30 -- X-MS-Has-Attach:
38, 30 -- X-MS-TNEF-Correlator:
38, 30 -- acceptlanguage: en-US
38, 30 -- Content-Type: text/plain; charset="us-ascii"
38, 30 -- MIME-Version: 1.0
38, 30 -- Content-Transfer-Encoding: 8bit
38, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n67K6JSJ029429
==============================End of - Headers==============================






==============================Original Headers==============================
58, 24 -- From nizets2-at-yahoo.com Wed Jul 8 02:46:04 2009
58, 24 -- Received: from web110812.mail.gq1.yahoo.com (web110812.mail.gq1.yahoo.com [67.195.13.235])
58, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n687k4pC002001
58, 24 -- for {microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 02:46:04 -0500
58, 24 -- Received: (qmail 34207 invoked by uid 60001); 8 Jul 2009 07:46:03 -0000
58, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1247039163; bh=SCNzCbUyjxBpTAu/5h4CVwD/cLuDLdjRS4sB48fmNAE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=uYqMJZPBdDlbJ11xHyENDbFL8s+WLPPJ61I6dxh7heVvFpDmMqKV8vgpiECl2NBK+qpYNhGl8/eUXwIsBTTO8Rq7faAL6948RarYos6fkiQsHoHTzyQLKyn2Wpun6hQPSgJ1qZjmCHqYjq33cFYu5VKlDwey1ZKzmk5SfnyPG0E=
58, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
58, 24 -- s=s1024; d=yahoo.com;
58, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
58, 24 -- b=j6z19T7cLqfHGczFI/a4VphIjqA5DmPhQihJ6w/0GB9svuYooYep9/idCUI29oa7SedprHWCdZ1/sq1qe3ZFEkfKVS6VKNcLrX8XCsg5v+1mfPW5CqXSkaRlLaM7jsIwHSd51FUl+7xIml+32kAoqMhUD4Ngs89ZQm6iyDDZ28Q=;
58, 24 -- Message-ID: {582876.33843.qm-at-web110812.mail.gq1.yahoo.com}
58, 24 -- X-YMail-OSG: c5jfPbUVM1kJQQdYcHFUwiiXXuvYwJ5dX0CdY8ceqhFV.Bxfwabi.M.kq7Yl4cQNIlfL2olJ8259XoxH7j0LYWVfHuBMbpkDCsxz82zaWAyyx1cNn2C_wPxfBioVONS5WX76BO94UJi2TVckn6fWIyUTUwKWIl95CCpgKd0zo2EDSX7GBRUCF.cC0oJnq3iWzhHDbjQ0jaw4JoRaVKME.dKDYjiNHYSXbDQ3OK70yFM8vWhYWBk_QdLTjGIqZ4i8rCtbTLW9iYv_azGWL5mqi2rvbDFmuNP9sUwf.mZkg6Z2xcvdXPueFpYunEWOLmqKXC548YS6E.HHMqmEIK7bQWCdxbZgRLmX20juQKBB8_M-
58, 24 -- Received: from [80.122.101.100] by web110812.mail.gq1.yahoo.com via HTTP; Wed, 08 Jul 2009 00:46:03 PDT
58, 24 -- X-Mailer: YahooMailRC/1358.21 YahooMailWebService/0.7.289.15
58, 24 -- References: {200907072009.n67K9WcX003792-at-ns.microscopy.com}
58, 24 -- Date: Wed, 8 Jul 2009 00:46:03 -0700 (PDT)
58, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
58, 24 -- Subject: Re: [Microscopy] price for new Transmission electron microscope
58, 24 -- To: microscopy-at-microscopy.com
58, 24 -- In-Reply-To: {200907072009.n67K9WcX003792-at-ns.microscopy.com}
58, 24 -- MIME-Version: 1.0
58, 24 -- Content-Type: text/plain; charset=iso-8859-1
58, 24 -- Content-Transfer-Encoding: 8bit
58, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n687k4pC002001
==============================End of - Headers==============================




From: Nicola.Weston-at-nottingham.ac.uk
Date: Wed, 8 Jul 2009 07:04:28 -0500
Subject: [Microscopy] Sem stereo images question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to all who responded to my plea for help. I think we are
ready to give it a go. You've all saved me a lot of research time with
your links.

Thanks again
Nikki

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.



==============================Original Headers==============================
5, 35 -- From Nicola.Weston-at-nottingham.ac.uk Wed Jul 8 07:04:26 2009
5, 35 -- Received: from gse-mta-23.emailfiltering.com (gse-mta-23-tx.emailfiltering.com [194.116.198.156])
5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68C4N8T017932
5, 35 -- for {microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 07:04:25 -0500
5, 35 -- Received: from smtp2.nottingham.ac.uk ([128.243.44.5])
5, 35 -- by gse-mta-23.emailfiltering.com with emfmta (version 3.7.1.44.1.r-3.2.3-libc2.3.2) vanilla id 13168688
5, 35 -- for microscopy-at-microscopy.com; Wed, 08 Jul 2009 13:04:21 +0100
5, 35 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11])
5, 35 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60)
5, 35 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} )
5, 35 -- id 1MOVsP-0001vE-MK
5, 35 -- for microscopy-at-microscopy.com; Wed, 08 Jul 2009 13:03:53 +0100
5, 35 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959);
5, 35 -- Wed, 8 Jul 2009 13:03:22 +0100
5, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 35 -- Content-class: urn:content-classes:message
5, 35 -- MIME-Version: 1.0
5, 35 -- Content-Type: text/plain;
5, 35 -- charset="us-ascii"
5, 35 -- Subject: Sem stereo images question
5, 35 -- Date: Wed, 8 Jul 2009 13:03:21 +0100
5, 35 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D9401C0C8D4-at-VUIEXCHA.ad.nottingham.ac.uk}
5, 35 -- X-MS-Has-Attach:
5, 35 -- X-MS-TNEF-Correlator:
5, 35 -- Thread-Topic: Sem stereo images question
5, 35 -- Thread-Index: Acn/xBbdW4RzsjpuR4C0GVzv3v8fKQ==
5, 35 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk}
5, 35 -- To: {microscopy-at-microscopy.com}
5, 35 -- X-OriginalArrivalTime: 08 Jul 2009 12:03:22.0955 (UTC) FILETIME=[177D99B0:01C9FFC4]
5, 35 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information
5, 35 -- X-UoN-MailScanner: Found to be clean
5, 35 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk
5, 35 -- X-Spam-Status: No
5, 35 -- Content-Transfer-Encoding: 8bit
5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n68C4N8T017932
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Wed, 8 Jul 2009 07:28:32 -0500
Subject: [Microscopy] Re: viaWWW: Tips or techniques for imaging magnetic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Christopher,

Ah yes... magnetic materials in the TEM! The bane of every materials
microscopist. It gives you great appreciation for the microscopists
in the 50s and 60s who did so much work on steels on the older scopes.

Basically, what you want to do is to reduce the amount of magnetic
material within the pole-piece of the TEM. FIB preparation is
probably the best in this regard. If you use ex-situ liftout, be
aware that soft iron will probably get pulled off the carbon film
because the magnetic forces exceed the van der Waals forces holding
it down. I've overcome this by overcoating the FIBbed sample with
carbon. In-situ FIB liftout attaches the sample to a post on a
support grid and in general works well for the magnetic samples I've
prepared.

For conventional thinning, try cutting a smaller disk of the iron and
glue it to a Cu or Mo support ring. You should be able to jet-polish
or ion mill the smaller disk. The support ring will build the sample
out to the standard 3mm diameter.

Good luck,
Henk


At 10:09 PM 07/07/09, cwinkler-at-drexel.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
12, 27 -- From colijn.1-at-osu.edu Wed Jul 8 07:28:32 2009
12, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68CSVUJ001655
12, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 07:28:31 -0500
12, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
12, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
12, 27 -- id {01NB2JM0N3CW965N36-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
12, 27 -- Wed, 08 Jul 2009 08:28:29 -0400 (EDT)
12, 27 -- Received: from HOC1.ecr6.ohio-state.edu
12, 27 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
12, 27 -- (PMDF V6.3-x18 #31556)
12, 27 -- with ESMTPA id {01NB2JM0CC9Q9657TK-at-ecr6.ohio-state.edu} ; Wed,
12, 27 -- 08 Jul 2009 08:28:28 -0400 (EDT)
12, 27 -- Date: Wed, 08 Jul 2009 08:30:14 -0400
12, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
12, 27 -- Subject: Re: [Microscopy] viaWWW: Tips or techniques for imaging magnetic
12, 27 -- materials in the
12, 27 -- In-reply-to: {200907080209.n6829VZc024024-at-ns.microscopy.com}
12, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
12, 27 -- To: cwinkler-at-drexel.edu
12, 27 -- Cc: Microscopy-at-microscopy.com
12, 27 -- Message-id: {01NB2JM0DFTC9657TK-at-ecr6.ohio-state.edu}
12, 27 -- MIME-version: 1.0
12, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed
12, 27 -- X-Env-From: auth/colijn.1-at-osu.edu
12, 27 -- References: {200907080209.n6829VZc024024-at-ns.microscopy.com}
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Wed, 8 Jul 2009 09:45:28 -0500
Subject: [Microscopy] price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wow - I am going to show some school visitors our Philips CM10 TEM and
the lovely colour 1950 RCA table top TEM right now.

Did they ever sell any?

Dave

-----Original Message-----
X-from: tom-at-TomKaye.com [mailto:tom-at-TomKaye.com]
Sent: 07 July 2009 21:03
To: David Patton

All,

Searching the net looking for info on permanent magnet SEM's I came
across this bit of trivia. A full brochure of RCA's 1950's tabletop SEM.
I don't think I have seen this on the listserv before so here is the
link.

http://www.smecc.org/rca_emt_tabletop.htm

Tom Kaye





-----Original Message-----
X-from: msa-at-lv-em.com [mailto:msa-at-lv-em.com]
Sent: Tuesday, July 07, 2009 12:44 PM
To: tom-at-tomkaye.com

True.

And on the website there's more information about what makes the LVEM5
unique - such as the benefits and limitations of 5kV in imaging and spec
prep.

Regarding 5 kV: We're pretty enthusiastic about that. It's one of the
factors what allows for the smaller footprint (that and the permanent
magnet
lenses) and much higher/better contrast for light elements - without
staining.

Ephram


----------------------------------
Ephram Shizgal

LVEM5 Team
Delong America Inc.
Montreal, Canada
www.lv-em.com




-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Tuesday, July 07, 2009 3:33 PM
To: msa-at-lv-em.com

While the LV-EM looks quite nice and is under USD 150k, note that it is
a 5kV permanent magnet electron microscope with whatever pluses and
minuses that entails.

Cheers,
Henk

At 03:03 PM 07/07/09, msa-at-lv-em.com wrote:



} -----------------------------------------------------------------------
} ----
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} on the list, but given the specific topic being discussed I think it is

} relevant and appropriate.
}
} Our TEM is a FEG (Schottky) that operates in vacuum and has 2 nm
resolution.
}
} It is a benchtop system.
}
} It has optional Scanning modes: SEM (3 nm. resolution) and STEM (2 nm.
} resolution).
}
} The TEM system price is 135,000 (SEM mode add 10,000, STEM add another
} 10,000).
} Add an additional 10,000 for our high end (2K x 2K cooled) CCD digital
} camera.
}
} So for 145,000 you can get a fully functional TEM with 2K digital
} camera (and there are no hidden charges...this price includes all
} components plus installation, training and 1 year service).
}
} The LVEM5 does NOT do everything that EVERY other EM does (no equipment
does
} everything and EM is no different) so you may want to visit our website

} to learn more. Or contact me directly.
}
} www.lv-em.com
}
}
} see you at M&M (booth 741)
}
}
} Ephram
}
}
} ----------------------------------
} Ephram Shizgal
}
} LVEM5 Team
} Delong America Inc.
}
} Montreal, Canada
}
} www.lv-em.com
}
}
}
}
} ==============================Original
Headers==============================
} 21, 19 -- From msa-at-lv-em.com Tue Jul 7 14:02:45 2009 21, 19 --
} Received: from relais.videotron.ca (relais.videotron.ca
} [24.201.245.36])
} 21, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n67J2jIJ004156
} 21, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
} 14:02:45 -0500
} 21, 19 -- MIME-version: 1.0
} 21, 19 -- Content-transfer-encoding: 7BIT 21, 19 -- Content-type:
} text/plain; charset=US-ASCII 21, 19 -- Received: from DF92QRH1
} ([74.56.174.139]) by VL-MO-MR004.ip.videotron.ca 21, 19 -- (Sun
} Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
} 21, 19 -- with ESMTP id {0KMF00728E88DGF0-at-VL-MO-MR004.ip.videotron.ca}

} for 21, 19 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:02:32
} -0400 (EDT) 21, 19 -- From: msa-at-lv-em.com 21, 19 -- To:
} Microscopy-at-microscopy.com 21, 19 -- Subject: RE: [Microscopy] price
} for new Transmission electron microscope 21, 19 -- Date: Tue, 07 Jul
} 2009 15:02:35 -0400 21, 19 -- Message-id:
} {006401c9ff35$7d94e7b0$78beb710$-at-com}
} 21, 19 -- X-Mailer: Microsoft Office Outlook 12.0 21, 19 --
} Thread-index: Acn/L00r3RRA4S6kSqiZoRJqKeiP3QAATc9wAAD1OnA=
} 21, 19 -- Content-language: en-us
} ==============================End of -
Headers==============================

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.


==============================Original
Headers==============================
8, 24 -- From colijn.1-at-osu.edu Tue Jul 7 14:27:25 2009 8, 24 --
Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu
[164.107.76.2])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id
n67JRO2B018834
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
14:27:25
-0500
8, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 8, 24 -- id
{01NB1JXYBIG09611CK-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
8, 24 -- Tue, 07 Jul 2009 15:27:18 -0400 (EDT) 8, 24 -- Received: from
HOC1.ecr6.ohio-state.edu 8, 24 -- (hoc1.ceof.ohio-state.edu
[164.107.76.179]) by er6s1.ecr6.ohio-state.edu 8, 24 -- (PMDF V6.3-x18
#31556) with ESMTPA id {01NB1JXXUFF2962W1S-at-ecr6.ohio-state.edu}
8, 24 -- for Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:27:18 -0400
(EDT)
8, 24 -- Date: Tue, 07 Jul 2009 15:29:03 -0400 8, 24 -- From: "Hendrik
O. Colijn" {colijn.1-at-osu.edu} 8, 24 -- Subject: Re: [Microscopy] RE:
price for new Transmission electron microscope 8, 24 -- In-reply-to:
{200907071903.n67J3sxN006293-at-ns.microscopy.com}
8, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 24 -- To:
Microscopy-at-microscopy.com 8, 24 -- Message-id:
{01NB1JXXVZWW962W1S-at-ecr6.ohio-state.edu}
8, 24 -- MIME-version: 1.0
8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 24 --
Content-type: text/plain; charset=us-ascii; format=flowed 8, 24 --
X-Env-From: auth/colijn.1-at-osu.edu 8, 24 -- References:
{200907071903.n67J3sxN006293-at-ns.microscopy.com}
==============================End of -
Headers==============================


==============================Original
Headers==============================
24, 21 -- From msa-at-lv-em.com Tue Jul 7 14:41:31 2009 24, 21 --
Received: from relais.videotron.ca (relais.videotron.ca
[24.201.245.36])
24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id
n67JfVs8000863
24, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
14:41:31 -0500
24, 21 -- MIME-version: 1.0
24, 21 -- Content-transfer-encoding: 7BIT 24, 21 -- Content-type:
text/plain; charset=US-ASCII 24, 21 -- Received: from DF92QRH1
([74.56.174.139]) by VL-MH-MR002.ip.videotron.ca 24, 21 -- (Sun
Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
24, 21 -- with ESMTP id {0KMF00MNLG14TFD0-at-VL-MH-MR002.ip.videotron.ca}
for 24, 21 -- Microscopy-at-microscopy.com; Tue, 07 Jul 2009 15:41:30
-0400 (EDT) 24, 21 -- From: msa-at-lv-em.com 24, 21 -- To:
Microscopy-at-microscopy.com 24, 21 -- References:
{200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- In-reply-to: {200907071932.n67JWYDM029015-at-ns.microscopy.com}
24, 21 -- Subject: RE: [Microscopy] price for new Transmission electron
microscope 24, 21 -- Date: Tue, 07 Jul 2009 15:41:32 -0400 24, 21 --
Message-id: {008601c9ff3a$eeface10$ccf06a30$-at-com}
24, 21 -- X-Mailer: Microsoft Office Outlook 12.0 24, 21 --
Thread-index: Acn/Oa5YMXTmQEA6RWWBxBSUHy9fQgAAJmDQ
24, 21 -- Content-language: en-us
==============================End of -
Headers==============================


==============================Original
Headers==============================
39, 22 -- From tom-at-TomKaye.com Tue Jul 7 14:59:58 2009 39, 22 --
Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com
[66.102.127.13])
39, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n67JxwUY015667
39, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jul 2009
14:59:58 -0500
39, 22 -- Received: from TKLAPTOP ([216.75.124.10]) by
w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959);
39, 22 -- Tue, 7 Jul 2009 14:59:57 -0500
39, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com} 39, 22 -- To:
{microscopy-at-microscopy.com} 39, 22 -- Subject: Permanent Magnet Tabletop
SEM---1950 Historical Note 39, 22 -- Date: Tue, 7 Jul 2009 13:00:00
-0700 39, 22 -- Message-ID:
{LHECKJAKKIOGKDBHHJMLCEJJDGAA.tom-at-tomkaye.com}
39, 22 -- MIME-Version: 1.0
39, 22 -- Content-Type: text/plain;
39, 22 -- charset="iso-8859-1"
39, 22 -- Content-Transfer-Encoding: 7bit 39, 22 -- X-Priority: 3
(Normal) 39, 22 -- X-MSMail-Priority: Normal 39, 22 -- X-Mailer:
Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 39, 22 --
In-Reply-To: {200907071944.n67JiOCQ006854-at-ns.microscopy.com}
39, 22 -- Importance: Normal
39, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 39,
22 -- X-OriginalArrivalTime: 07 Jul 2009 19:59:57.0639 (UTC)
FILETIME=[80D65D70:01C9FF3D] ==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
56, 34 -- From David.Patton-at-uwe.ac.uk Wed Jul 8 09:45:28 2009
56, 34 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
56, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n68EjRpV026473
56, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 09:45:27 -0500
56, 34 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
56, 34 -- id 710e_f96db7ae_6bcd_11de_98ee_00142223915c;
56, 34 -- Wed, 08 Jul 2009 15:45:26 +0100
56, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
56, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
56, 34 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
56, 34 -- 2005)) with ESMTP id {0KMG00EY0WZOJN-at-mta01.uwe.ac.uk} for
56, 34 -- Microscopy-at-microscopy.com; Wed, 08 Jul 2009 15:45:26 +0100 (BST)
56, 34 -- Date: Wed, 08 Jul 2009 15:45:14 +0100
56, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk}
56, 34 -- Subject: RE: [Microscopy] Permanent Magnet Tabletop SEM---1950 Historical Note
56, 34 -- In-reply-to: {200907072002.n67K2jQx021302-at-ns.microscopy.com}
56, 34 -- To: tom-at-TomKaye.com
56, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB04D88699-at-egen-uwe01}
56, 34 -- MIME-version: 1.0
56, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
56, 34 -- Content-type: text/plain; charset=us-ascii
56, 34 -- Content-class: urn:content-classes:message
56, 34 -- Thread-topic: [Microscopy] Permanent Magnet Tabletop SEM---1950 Historical Note
56, 34 -- Thread-index: Acn/PmvKoBy9vmkBTxyCzlb8w1VxggAmv3rQ
56, 34 -- X-MS-Has-Attach:
56, 34 -- X-MS-TNEF-Correlator:
56, 34 -- References: {200907072002.n67K2jQx021302-at-ns.microscopy.com}
56, 34 -- X-NAIMIME-Disclaimer: 1
56, 34 -- X-NAIMIME-Modified: 1
56, 34 -- X-NAI-Spam-Score: 0
56, 34 -- X-NAI-Spam-Rules: 1 Rules triggered
56, 34 -- RV3313=0
56, 34 -- Content-Transfer-Encoding: 8bit
56, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n68EjRpV026473
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Wed, 8 Jul 2009 10:51:36 -0500
Subject: [Microscopy] Philips pPerm Mag TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Philips Co. Also manufactured a TEM with permanent magnetic
lenses: their Model EM-75, about 1955. As I recall, magnification
was varied by changing the spacing between the pole pieces of the
projector lens. I have a photo of this instrument, and would be
willing to send a copy to anyone that would like to have it.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
1, 15 -- From bigelow-at-umich.edu Wed Jul 8 10:51:35 2009
1, 15 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160])
1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68FpYjV011560
1, 15 -- for {microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 10:51:34 -0500
1, 15 -- Received: FROM [76.226.73.83] (adsl-76-226-73-83.dsl.sfldmi.sbcglobal.net [76.226.73.83])
1, 15 -- By skycaptain.mr.itd.umich.edu ID 4A54C083.E595F.595 ;
1, 15 -- Authuser bigelow;
1, 15 -- 8 Jul 2009 11:51:32 EDT
1, 15 -- Mime-Version: 1.0
1, 15 -- Message-Id: {p06240801c67a6fcd6103-at-[76.226.73.83]}
1, 15 -- Date: Wed, 8 Jul 2009 11:51:25 -0400
1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 15 -- Subject: [Microscopy] Philips pPerm Mag TEM
1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jmardinly-at-gmail.com
Date: Wed, 8 Jul 2009 12:32:49 -0500
Subject: [Microscopy] Re: Philips pPerm Mag TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Actually there is a photo of the EM-75 on the cover of the Ted Pella
catalog as well as the home page of their web site.

John Mardinly
Sent from my iPhone.

On Jul 8, 2009, at 9:00 AM, bigelow-at-umich.edu wrote:

}
}
}
} ---
} ---
} ----------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---
} ---
} ----------------------------------------------------------------------
}
} The Philips Co. Also manufactured a TEM with permanent magnetic
} lenses: their Model EM-75, about 1955. As I recall, magnification
} was varied by changing the spacing between the pole pieces of the
} projector lens. I have a photo of this instrument, and would be
} willing to send a copy to anyone that would like to have it.
} --
} Wilbur C. Bigelow, Professor Emeritus
} Materials Sci. & Engr., Univ. of Michigan
} Ann Arbor, Michigan 48109-2136
} e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-975-0858
} Address mail to: 2911 Whittier Court
} Ann Arbor, MI 48104-6731
}
} ==============================Original
} Headers==============================
} 1, 15 -- From bigelow-at-umich.edu Wed Jul 8 10:51:35 2009
} 1, 15 -- Received: from skycaptain.mr.itd.umich.edu
} (smtp.mail.umich.edu [141.211.93.160])
} 1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n68FpYjV011560
} 1, 15 -- for {microscopy-at-microscopy.com} ; Wed, 8 Jul 2009
} 10:51:34 -0500
} 1, 15 -- Received: FROM [76.226.73.83]
} (adsl-76-226-73-83.dsl.sfldmi.sbcglobal.net [76.226.73.83])
} 1, 15 -- By skycaptain.mr.itd.umich.edu ID 4A54C083.E595F.595 ;
} 1, 15 -- Authuser bigelow;
} 1, 15 -- 8 Jul 2009 11:51:32 EDT
} 1, 15 -- Mime-Version: 1.0
} 1, 15 -- Message-Id: {p06240801c67a6fcd6103-at-[76.226.73.83]}
} 1, 15 -- Date: Wed, 8 Jul 2009 11:51:25 -0400
} 1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
} 1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
} 1, 15 -- Subject: [Microscopy] Philips pPerm Mag TEM
} 1, 15 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
4, 43 -- From jmardinly-at-gmail.com Wed Jul 8 12:32:49 2009
4, 43 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.169])
4, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68HWn6T030206
4, 43 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 12:32:49 -0500
4, 43 -- Received: by wf-out-1314.google.com with SMTP id 28so2044864wfa.21
4, 43 -- for {Microscopy-at-microscopy.com} ; Wed, 08 Jul 2009 10:32:48 -0700 (PDT)
4, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=domainkey-signature:received:received:references:message-id:from:to
4, 43 -- :in-reply-to:content-type:content-transfer-encoding:x-mailer
4, 43 -- :mime-version:subject:date:cc;
4, 43 -- bh=ghzqeMklnce1HeWtN9EdUYbKHvYMhmU5CeSCnxvaMew=;
4, 43 -- b=GuSaiu9xbGX78t77QvsocIyTs9yR/tOp591/D1PhlBSM702nKKRlbZxTP/31mEbbZy
4, 43 -- aafdzXOto/7x1KIk2BCXW64axW1Lrzl9fnd/gXxLPPTt9xEjYwvbCpNS9fhOneSZruVO
4, 43 -- HFAnFqCXQae8bD+4n/98JOFv7RdnH7bXED/SE=
4, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 43 -- d=gmail.com; s=gamma;
4, 43 -- h=references:message-id:from:to:in-reply-to:content-type
4, 43 -- :content-transfer-encoding:x-mailer:mime-version:subject:date:cc;
4, 43 -- b=tp9lY2BW4ErHOOTVGrD8BbWWrMrxwZq0LUOpCQ2MzzkXD38URFTetxJKk30QkCBSj7
4, 43 -- fndFCYaXOydIxnqlGqsIhSwpB+O+ubqP8uJJmsqskNQDWH9tWpfLTOH9Pv4Oz/lj0Bug
4, 43 -- mlaVS38tap13ikAulm0bqzStwn0L5Nh3qRYHM=
4, 43 -- Received: by 10.143.3.4 with SMTP id f4mr2329247wfi.62.1247074368623;
4, 43 -- Wed, 08 Jul 2009 10:32:48 -0700 (PDT)
4, 43 -- Received: from ?192.168.1.102? (75-25-131-32.lightspeed.sjcpca.sbcglobal.net [75.25.131.32])
4, 43 -- by mx.google.com with ESMTPS id 30sm8076037wfd.23.2009.07.08.10.32.47
4, 43 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 43 -- Wed, 08 Jul 2009 10:32:48 -0700 (PDT)
4, 43 -- References: {200907081600.n68G04Cf025026-at-ns.microscopy.com}
4, 43 -- Message-Id: {10679C5D-7AAD-4392-8EC6-107615F7C125-at-gmail.com}
4, 43 -- From: John Mardinly {jmardinly-at-gmail.com}
4, 43 -- To: "Wilbur C. Bigelow" {bigelow-at-umich.edu}
4, 43 -- In-Reply-To: {200907081600.n68G04Cf025026-at-ns.microscopy.com}
4, 43 -- Content-Type: text/plain;
4, 43 -- charset=us-ascii;
4, 43 -- format=flowed;
4, 43 -- delsp=yes
4, 43 -- Content-Transfer-Encoding: 7bit
4, 43 -- X-Mailer: iPhone Mail (5H11)
4, 43 -- Mime-Version: 1.0 (iPhone Mail 5H11)
4, 43 -- Subject: Re: [Microscopy] Philips pPerm Mag TEM
4, 43 -- Date: Wed, 8 Jul 2009 10:32:42 -0700
4, 43 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
==============================End of - Headers==============================




From: DennisH658-at-aol.com
Date: Wed, 8 Jul 2009 14:53:29 -0500
Subject: [Microscopy] Sem stereo images question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

You may also want to check out StereoPhoto Maker
(_http://stereo.jpn.org/eng/stphmkr/_ (http://stereo.jpn.org/eng/stphmkr/) ). This program is highly
rated by several stereo photographic societies, and can produce pictures in
several 3-D formats. It can also adjust pictures that are not quite
properly aligned. Oh yes... It's also freeware.

Dennis


----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hello Nikky !

It is quite easy to get stunning stereo pictures in a SEM. Following is my
receipt to get excellent stereo photomicrograph.

1) First of all, be sure that your specimen holder is oriented (playing on
the
rotation) in such a way that when you will tilt your specimen, this tilting
will be along a vertical line (North-South direction).

2) Take a first picture of the area of interest.

3) Take care to carefully note the exact position of some points of
interest in
this first picture (particularly their vertical positions = Y coordinate).
If
your SEM is equipped with a conventional CRT screen, you can use an
erasable
marker to note the position of some remarkable details of this first photo
directly on the screen. With the new digital generation of SEM equipped
with
LCD monitor, most of their software will allow you to use the “ruler†or
“marker†command to clearly pinpoint two remarkable details on this first
photo. Or put an acetate sheet on the LCD screen and use an erasable
marker on
this sheet.

4) Tilt your specimen in the clockwise direction by 7, 8 or 9 degrees. When
doing that, the area of interest will be shifted. So, go to 8 degrees (for
example) by small steps when keeping your area of interest in view by
playing
on the X and Y positions of the sample holder at each step.

5) Refocus your area of interest. The best way is to take a detail in the
center
of this area and to focus it by playing on the Z position of the specimen.
If
you are working at small magnifications (e.g. { 2 K) and with a large
depth of
field (high working distance WD associated with a small objective
aperture),
you can refocus the area of interest with the “Focus†knob of the SEM; it
is
easier than to refocus with the Z position of the specimen.

6) By playing with the X and Y positions of the area of interest, be sure
that
the remarkable details already pinpointed at the step 3 are now located at
the
same positions that they have in the first picture (taken at step 2). Their
vertical position is particularly important. Most generally, their
horizontal
distance will have changed. So, keep their new horizontal position in
between
the previous one.

7) Take the second picture of your area of interest.

Et VOILA! You are done! Now, to view your area of interest in stereo, you
need
a stereo viewer under which you place your two views (left and right) and
play
on their positions up until you get a stereo image.

You can also prepare an anaglyph with these two photos, the first one being
tinted in red and the second one tinted in blue (or green). To do that, I
suggest you to download the freeware ANAMAKER
(http://www.stereoeye.jp/software/index_e.html).

It is easy to prepare an anaglyph with this program which allows
correcting some
X and Y misalignments between the two photos. The anaglyph generated by
Anamaker
can now be viewed through Red-Blue (or Red-Green) glasses.

Good lucks and enjoy the stereo world.

Jean-Paul Bailon


} Greetings all
} I have a student who wishes to do some stereo images, which I have no
} experience of. Could you tell me the best software to use to merge the 2
} tilted images. Or some good references.
}
} Many thanks
} Nikki

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Prof. Jean-Paul Baïlon jean-paul.bailon-at-polymtl.ca
Dépt. de Génie mécanique Tél.: 1(514) 340 4711, p. 4260
École Polytechnique Fax.: 1(514) 340 4468
CP6079, Succ. Centre-Ville
Montréal (QC) Canada H3C 3A7
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

**************Summer concert season is here! Find your favorite artists on
tour at TourTracker.com. (http://www.tourtracker.com/?ncid=eml
cntusmusi00000006)


==============================Original Headers==============================
22, 22 -- From DennisH658-at-aol.com Wed Jul 8 14:53:28 2009
22, 22 -- Received: from imr-da04.mx.aol.com (imr-da04.mx.aol.com [205.188.105.146])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68JrSaL019075
22, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 14:53:28 -0500
22, 22 -- Received: from imo-ma02.mx.aol.com (imo-ma02.mx.aol.com [64.12.78.137])
22, 22 -- by imr-da04.mx.aol.com (8.14.1/8.14.1) with ESMTP id n68JrPoO028746
22, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 15:53:25 -0400
22, 22 -- Received: from DennisH658-at-aol.com
22, 22 -- by imo-ma02.mx.aol.com (mail_out_v40_r1.5.) id w.cdc.4f628a19 (65099)
22, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 15:53:17 -0400 (EDT)
22, 22 -- From: DennisH658-at-aol.com
22, 22 -- Message-ID: {cdc.4f628a19.37865346-at-aol.com}
22, 22 -- Date: Wed, 8 Jul 2009 15:53:42 EDT
22, 22 -- Subject: Sem stereo images question
22, 22 -- To: Microscopy-at-microscopy.com
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain; charset="UTF-8"
22, 22 -- Content-Language: en
22, 22 -- X-Mailer: AOL 9.5 sub 29
22, 22 -- X-Spam-Flag: NO
22, 22 -- Content-Transfer-Encoding: 8bit
22, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n68JrSaL019075
==============================End of - Headers==============================




From: bengu-at-fen.bilkent.edu.tr
Date: Wed, 8 Jul 2009 16:46:11 -0500
Subject: [Microscopy] Question regarding JEOL 6400

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

We are trying to upgrade a JEOL 6400. We would like acquire quality
digital images using a third party solution from this SEM.

Anybody who can provide some info on the available options ? Pros and cons
will be much appreciated. Also, how much did you spend ?

Thanx

Erman




==============================Original Headers==============================
9, 22 -- From bengu-at-fen.bilkent.edu.tr Wed Jul 8 16:46:11 2009
9, 22 -- Received: from ispinoz.bcc.bilkent.edu.tr (ispinoz.bcc.bilkent.edu.tr [139.179.10.240])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n68Lk9ii005807
9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 8 Jul 2009 16:46:10 -0500
9, 22 -- Received: from newmail.bilkent.edu.tr (unknown [192.168.10.203])
9, 22 -- by ispinoz.bcc.bilkent.edu.tr (Postfix) with ESMTP id EC27C6413
9, 22 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 00:46:24 +0300 (EEST)
9, 22 -- Received: from 139.179.97.107
9, 22 -- (SquirrelMail authenticated user bengu-at-fen.bilkent.edu.tr)
9, 22 -- by newmail.bilkent.edu.tr with HTTP;
9, 22 -- Thu, 9 Jul 2009 00:46:10 +0300 (EEST)
9, 22 -- Message-ID: {4749.139.179.97.107.1247089570.squirrel-at-newmail.bilkent.edu.tr}
9, 22 -- Date: Thu, 9 Jul 2009 00:46:10 +0300 (EEST)
9, 22 -- Subject: Question regarding JEOL 6400
9, 22 -- From: "Erman Bengu" {bengu-at-fen.bilkent.edu.tr}
9, 22 -- To: microscopy-at-microscopy.com
9, 22 -- User-Agent: SquirrelMail/1.4.13
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain;charset=iso-8859-1
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-Priority: 3 (Normal)
9, 22 -- Importance: Normal
==============================End of - Headers==============================




From: emlabservices-at-cox.net
Date: Thu, 9 Jul 2009 07:22:40 -0500
Subject: [Microscopy] Re: price for new Transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

In response to the recent thread on the purchase price of a new TEM, I would
add that some may be pleasantly surprised after researching the secondary or
used instrument market. For $150K you can get plenty of uncompromised TEM,
EDX, digital imaging (yes, CCD camera), electro-magnetic/electro-static
lens-based instrumentation. The current market conditions have never been
better for purchasing this type of equipment.

While much emphasis is placed on the negotiated terms and purchase price of
instrumentation, I would point out that long after these issues fade and
after the sale or transaction passes, what matters most is future support,
service, parts availability, and other necessary resources to sustain your
research and the instrument's intended application for years to come.

One point or suggestion was made to contact the OEM's for availability of
instruments. It has been my experience the OEM 's are primarily involved in
bringing new equipment to market and their interests are sales along these
lines.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



==============================Original Headers==============================
7, 33 -- From emlabservices-at-cox.net Thu Jul 9 07:22:40 2009
7, 33 -- Received: from eastrmmtao104.cox.net (eastrmmtao104.cox.net [68.230.240.46])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69CMdk7000342
7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 07:22:40 -0500
7, 33 -- Received: from eastrmimpo02.cox.net ([68.1.16.120])
7, 33 -- by eastrmmtao104.cox.net
7, 33 -- (InterMail vM.7.08.02.01 201-2186-121-102-20070209) with ESMTP
7, 33 -- id {20090709122239.XBVW5576.eastrmmtao104.cox.net-at-eastrmimpo02.cox.net}
7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 08:22:39 -0400
7, 33 -- Received: from EMLabServices ([68.103.21.144])
7, 33 -- by eastrmimpo02.cox.net with bizsmtp
7, 33 -- id DoNe1c00C36XJGc02oNfr4; Thu, 09 Jul 2009 08:22:39 -0400
7, 33 -- X-VR-Score: 0.00
7, 33 -- X-Authority-Analysis: v=1.0 c=1 a=q1M_cKjS8ioA:10 a=7lK6D5y0AAAA:8
7, 33 -- a=Ed-285B1apY9YOpFAF4A:9 a=8_8kEpb3kuadRVkIQ2sA:7
7, 33 -- a=aVYF7M_YvNjJlHIfOpd2Jyo6negA:4 a=5FnZdabJD9cA:10 a=Ukfy9IDRlMMA:10
7, 33 -- a=QdrHNQEAe2YA:10
7, 33 -- X-CM-Score: 0.00
7, 33 -- Message-ID: {CC7CBA98FE844A039192DF02022B2A65-at-EMLabServices}
7, 33 -- From: "EM Lab Services" {emlabservices-at-cox.net}
7, 33 -- To: {Microscopy-at-microscopy.com}
7, 33 -- Subject: [Microscopy] Re: price for new Transmission electron microscope
7, 33 -- Date: Thu, 9 Jul 2009 06:22:43 -0600
7, 33 -- MIME-Version: 1.0
7, 33 -- Content-Type: text/plain;
7, 33 -- format=flowed;
7, 33 -- charset="iso-8859-1";
7, 33 -- reply-type=original
7, 33 -- Content-Transfer-Encoding: 7bit
7, 33 -- X-Priority: 3
7, 33 -- X-MSMail-Priority: Normal
7, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
7, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: apobanz-at-gmail.com
Date: Thu, 9 Jul 2009 07:22:56 -0500
Subject: [Microscopy] viaWWW: SEM image optimization - contrast & brightness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both apobanz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: apobanz-at-gmail.com
Name: Alan Pobanz

Title-Subject: [Filtered] SEM image optimization - contrast & brightness

Question: Regarding adjusting contrast and brightness in SEM imaging,
Brian Tracy, in a microscopy seminar, gave an excellent description
about mimicking Ansel Adams B&W photos: 5% of the image should be
saturated black, 5% should be saturated white, and the remainder
should exhibit every possible gray level. I have a coworker that
thinks this is crazy (she uses very high contrast and no brightness).
Can you point me to some good reference material that describes how
best to adjust contrast and brightness in SEM images?
Thanks!
Alan

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Thu Jul 9 07:22:56 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69CMtrX000635
5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 07:22:55 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240800c67b9177f95a-at-[206.69.208.22]}
5, 11 -- Date: Thu, 9 Jul 2009 07:22:54 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: apobanz-at-gmail.com (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: SEM image optimization - contrast & brightness
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: joe.kintz-at-stress.com
Date: Thu, 9 Jul 2009 08:49:27 -0500
Subject: [Microscopy] viaWWW: SEM image optimization - contrast & brightness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Alan:

My Tescan SEM has a histogram feature that makes it very easy to see what
portion of the image is at all of the various brightness levels. What I do
is adjust the brightness and contrast until none of the darks and none of
the brights are outside of the range of the histogram, meaning that this
data would be lost from the image. Then I increase or decrease the gamma
setting until the image looks pleasing to my eye. I find this method to be
more reliable than trusting my eye alone, particularly if your monitor
hasn't been calibrated.

Joe

Joseph Kintz, P.E.
Staff Consultant
Stress Engineering Services, Inc. - An Employee Owned Company
13800 Westfair East Drive
Houston, TX 77041-1101

Office: 281-955-2900, ext. 266
Home: 281-861-9700
Mobile: 281-851-0591

joe.kintz-at-stress.com
www.stress.com

-----Original Message-----
X-from: apobanz-at-gmail.com [mailto:apobanz-at-gmail.com]
Sent: Thursday, July 09, 2009 7:36 AM
To: joe.kintz-at-stress.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both apobanz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: apobanz-at-gmail.com
Name: Alan Pobanz

Title-Subject: [Filtered] SEM image optimization - contrast & brightness

Question: Regarding adjusting contrast and brightness in SEM imaging,
Brian Tracy, in a microscopy seminar, gave an excellent description
about mimicking Ansel Adams B&W photos: 5% of the image should be
saturated black, 5% should be saturated white, and the remainder
should exhibit every possible gray level. I have a coworker that
thinks this is crazy (she uses very high contrast and no brightness).
Can you point me to some good reference material that describes how
best to adjust contrast and brightness in SEM images?
Thanks!
Alan

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Thu Jul 9 07:22:56 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n69CMtrX000635
5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 07:22:55
-0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240800c67b9177f95a-at-[206.69.208.22]}
5, 11 -- Date: Thu, 9 Jul 2009 07:22:54 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: apobanz-at-gmail.com (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: SEM image optimization - contrast & brightness
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
14, 21 -- From Joe.Kintz-at-stress.com Thu Jul 9 08:49:27 2009
14, 21 -- Received: from mxgate.stress.com (mxgate.stress.com [12.96.4.229])
14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69DnREe000769
14, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 08:49:27 -0500
14, 21 -- Received: from jkintz ([172.16.12.84]) by mail.stress.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 9 Jul 2009 08:49:22 -0500
14, 21 -- From: "Joe Kintz" {joe.kintz-at-stress.com}
14, 21 -- To: {apobanz-at-gmail.com}
14, 21 -- Cc: {microscopy-at-microscopy.com}
14, 21 -- References: {200907091235.n69CZVpw027685-at-ns.microscopy.com}
14, 21 -- Subject: RE: [Microscopy] viaWWW: SEM image optimization - contrast & brightness
14, 21 -- Date: Thu, 9 Jul 2009 08:49:21 -0500
14, 21 -- Message-ID: {001f01ca009c$0ff23470$540c10ac-at-stress.com}
14, 21 -- MIME-Version: 1.0
14, 21 -- Content-Type: text/plain;charset="us-ascii"
14, 21 -- Content-Transfer-Encoding: 7bit
14, 21 -- X-Mailer: Microsoft Office Outlook 11
14, 21 -- Thread-Index: AcoAkcDlLwpWaQ0BT7KiQPzQaQc2QwACZ4Sw
14, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
14, 21 -- In-Reply-To: {200907091235.n69CZVpw027685-at-ns.microscopy.com}
14, 21 -- X-OriginalArrivalTime: 09 Jul 2009 13:49:22.0122 (UTC) FILETIME=[10433AA0:01CA009C]
14, 21 -- X-SCA-Stop: [cust-96647FDDB5F74CA0B2173CACB4D94086,18,39,0]
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 9 Jul 2009 08:57:30 -0500
Subject: [Microscopy] viaWWW: SEM image optimization - contrast & brightness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alan,
For aesthetics it may be nice to have 5% black and 5% white, but for
technical images, that means that 10% of your image has no information.
Unless there are areas that you are absolutely sure are of no interest, my
feeling has always been that the entire ORIGINAL image should be in the gray
range. In digital terms, from 1-254 on a scale of 0-255. You can always
play with the aesthetics in PhotoShop afterwards while keeping the original
file protected.

Back in the dark ages I would slightly over-expose my Polaroid Type 55
positives because it yielded a denser negative that made better prints, but
I still tried not to saturate the negative either white or black. I could
then play with contrast in the dark room, but still had 100% of the
information on the negative.

I guess the question is this: is your coworker "making pictures" for art or
"taking pictures" for technical information? Either one is fine, but each
has a different end in mind and uses different techniques.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: apobanz-at-gmail.com [mailto:apobanz-at-gmail.com]
Sent: Thursday, July 09, 2009 8:26 AM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both apobanz-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: apobanz-at-gmail.com
Name: Alan Pobanz

Title-Subject: [Filtered] SEM image optimization - contrast & brightness

Question: Regarding adjusting contrast and brightness in SEM imaging,
Brian Tracy, in a microscopy seminar, gave an excellent description
about mimicking Ansel Adams B&W photos: 5% of the image should be
saturated black, 5% should be saturated white, and the remainder
should exhibit every possible gray level. I have a coworker that
thinks this is crazy (she uses very high contrast and no brightness).
Can you point me to some good reference material that describes how
best to adjust contrast and brightness in SEM images?
Thanks!
Alan

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Thu Jul 9 07:22:56 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n69CMtrX000635
5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 07:22:55
-0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240800c67b9177f95a-at-[206.69.208.22]}
5, 11 -- Date: Thu, 9 Jul 2009 07:22:54 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: apobanz-at-gmail.com (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: SEM image optimization - contrast & brightness
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
19, 25 -- From kenconverse-at-qualityimages.biz Thu Jul 9 08:57:30 2009
19, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69DvU2o011863
19, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 08:57:30 -0500
19, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
19, 25 -- with ESMTP
19, 25 -- id {20090709135729427.QBUI19322-at-cdptpa-omta02.mail.rr.com} ;
19, 25 -- Thu, 9 Jul 2009 13:57:29 +0000
19, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
19, 25 -- To: {apobanz-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com}
19, 25 -- Subject: RE: [Microscopy] viaWWW: SEM image optimization - contrast & brightness
19, 25 -- Date: Thu, 9 Jul 2009 09:57:19 -0400
19, 25 -- Message-ID: {B0E24C9DF35D490CB331E86FB5F0BFD0-at-Ken}
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Type: text/plain;
19, 25 -- charset="us-ascii"
19, 25 -- X-Priority: 3 (Normal)
19, 25 -- X-MSMail-Priority: Normal
19, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
19, 25 -- Importance: Normal
19, 25 -- In-Reply-To: {200907091226.n69CQC3n008275-at-ns.microscopy.com}
19, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 25 -- Thread-Index: AcoAkHt0py1EzqLTQOSHWeCGuc2TMAAClefg
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n69DvU2o011863
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 9 Jul 2009 09:02:19 -0500
Subject: [Microscopy] Re: viaWWW: SEM image optimization - contrast &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't follow this set of guidelines. Adams' Zone system
was based on expanding the tonal range of final prints.
The first phase is The Negative while the second phase is
The Print. Typically what I have done (in film era) is
to over expose the neg and then under develop it. This
significantly elongates the S-shape curve of the negative
contrast range. In this situation, the middle part of the
density area is elongated and results in more data for
gray shades. Blown out highlights are useless as are under
exposed black areas. The negative is first and foremost of
importance. Typically over exposure of the neg by one stop
and under development by 20% resulted in an excellent negative.
Printing was then fairly routine.

Why not opposite? Under expose the neg and over develop the print?
If no light (data) on the film, it cannot be created by
over developing. Once the exposure and development critera
are set, Zone III is always the central target and that yields
two zones below and above Zone III. Plus, all five zones
are in a near perfect linear region.

In a digital arena, my strategy is to process in 16-bits with
either 12 or 14 native active bits. Input gamma adjustment is
essential for creating the initial TIFF image. Most SEMs will
do a contrast and brightness display with an upper line and lower
line. With contrast adjusted to maximize peaks and valleys and
brightness adjusted to keep all within the lines, that is a good
starting point. Then look at the image. A black area is not going
to show any info, hence, it needs more brightness. A white area
also will show no info and thus, needs less contrast.

A poor SEM image is easy to spot. Point and shoot does not apply.
The eyes, hands and brain have to be working in sync to get a
final crisp and useful photo/pix. Polaroids had limited tonal
range and I see no pain about their loss.

gary g.

At 05:24 AM 7/9/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 21 -- From gary-at-gaugler.com Thu Jul 9 09:02:19 2009
10, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n69E2IkR024825
10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 09:02:18 -0500
10, 21 -- Message-Id: {200907091402.n69E2IkR024825-at-ns.microscopy.com}
10, 21 -- Received: (qmail 7427 invoked from network); 9 Jul 2009 06:51:45 -0700
10, 21 -- Received: by simscan 1.1.0 ppid: 7424, pid: 7425, t: 0.0914s
10, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 21 -- by smtp2 with SMTP; 9 Jul 2009 06:51:45 -0700
10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 21 -- Date: Thu, 09 Jul 2009 07:02:09 -0700
10, 21 -- To: apobanz-at-gmail.com
10, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 21 -- Subject: Re: [Microscopy] viaWWW: SEM image optimization - contrast &
10, 21 -- brightness
10, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 21 -- In-Reply-To: {200907091224.n69COtWa005161-at-ns.microscopy.com}
10, 21 -- References: {200907091224.n69COtWa005161-at-ns.microscopy.com}
10, 21 -- Mime-Version: 1.0
10, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 9 Jul 2009 10:50:57 -0500
Subject: [Microscopy] RE: viaWWW: SEM image optimization - contrast & brightness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think it is not a good advice to throw away 10% of your image in
advance.

I believe we are talking about digital images. My strategy for getting
better looking images is to obtain SEM image utilizing wave form monitor
so that all gray levels are within acquisition range. To be on the safe
side (not to get truncated histogram) I let highest and lowest levels of
signal to be within acceptable range, not exactly on its thresholds.
Than comes Photoshop. With "levels" tool I can eliminate empty levels
(moving left and right slider) and expand histogram. Of course, I am
loosing some of the levels, but for 8-bit image we have 256 gray levels
while human eye can distinguish only about 150 levels. So, we have some
extra. I almost never use Photoshop's brightness/contrast corrections,
but sometimes I do use a bit of gamma correction.

In some special cases I prefer a truncated histogram, mostly during
acquisition, not in Photoshop manipulations. For example, if I have an
edge effect (and for some purposes do not want to lower high voltage), I
can allow signal level from edges go well above white limit of a wave
monitor, so that a histogram of the rest of the picture will be in
acceptable range. On the other side, when I have some objects
(particles, cells, etc.) on dark substrate, I can allow signal from
substrate go below black limits of a wave monitor and get a picture of
objects on black background.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: apobanz-at-gmail.com [mailto:apobanz-at-gmail.com]
} Sent: Thursday, July 09, 2009 7:24 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] viaWWW: SEM image optimization -
} contrast & brightness
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} This Question/Comment was submitted to the Microscopy
} Listserver using the WWW based Form at
} http://www.microscopy.com/MLFormMail.html
} --------------------------------------------------------------
} -------------
} Remember this posting is most likely not from a Subscriber,
} so when replying
} please copy both apobanz-at-gmail.com as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------
} -------------
}
} Email: apobanz-at-gmail.com
} Name: Alan Pobanz
}
} Title-Subject: [Filtered] SEM image optimization - contrast &
} brightness
}
} Question: Regarding adjusting contrast and brightness in SEM
} imaging, Brian Tracy, in a microscopy seminar, gave an
} excellent description about mimicking Ansel Adams B&W photos:
} 5% of the image should be saturated black, 5% should be
} saturated white, and the remainder should exhibit every
} possible gray level. I have a coworker that thinks this is
} crazy (she uses very high contrast and no brightness).
} Can you point me to some good reference material that
} describes how best to adjust contrast and brightness in SEM images?
} Thanks!
} Alan
}
} Login Host: 63.163.107.100
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
} 5, 11 -- From zaluzec-at-microscopy.com Thu Jul 9 07:22:56 2009
} 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n69CMtrX000635
} 5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul
} 2009 07:22:55 -0500
} 5, 11 -- Mime-Version: 1.0
} 5, 11 -- Message-Id: {p06240800c67b9177f95a-at-[206.69.208.22]}
} 5, 11 -- Date: Thu, 9 Jul 2009 07:22:54 -0500 5, 11 -- To:
} microscopy-at-microscopy.com 5, 11 -- From: apobanz-at-gmail.com
} (by way of MicroscopyListserver) 5, 11 -- Subject: viaWWW:
} SEM image optimization - contrast & brightness 5, 11 --
} Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
9, 25 -- From DusevichV-at-umkc.edu Thu Jul 9 10:50:57 2009
9, 25 -- Received: from kc-msxproto3.kc.umkc.edu (smtp2.exchange.umkc.edu [134.193.44.10])
9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69FovsC019225
9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 10:50:57 -0500
9, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 25 -- Thu, 9 Jul 2009 10:50:56 -0500
9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 25 -- Content-class: urn:content-classes:message
9, 25 -- MIME-Version: 1.0
9, 25 -- Content-Type: text/plain;
9, 25 -- charset="US-ASCII"
9, 25 -- Subject: RE: [Microscopy] viaWWW: SEM image optimization - contrast & brightness
9, 25 -- Date: Thu, 9 Jul 2009 10:50:55 -0500
9, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB889-at-KC-MSX1.kc.umkc.edu}
9, 25 -- In-Reply-To: {200907091223.n69CNW8Z002104-at-ns.microscopy.com}
9, 25 -- X-MS-Has-Attach:
9, 25 -- X-MS-TNEF-Correlator:
9, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM image optimization - contrast & brightness
9, 25 -- thread-index: AcoAkBQNUpxRqMNESBmffseRokdEGQAHMYcQ
9, 25 -- References: {200907091223.n69CNW8Z002104-at-ns.microscopy.com}
9, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
9, 25 -- To: {apobanz-at-gmail.com} , {Microscopy-at-microscopy.com}
9, 25 -- X-OriginalArrivalTime: 09 Jul 2009 15:50:56.0559 (UTC) FILETIME=[0C15FBF0:01CA00AD]
9, 25 -- Content-Transfer-Encoding: 8bit
9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n69FovsC019225
==============================End of - Headers==============================




From: velliyka-at-umdnj.edu
Date: Thu, 9 Jul 2009 11:08:31 -0500
Subject: [Microscopy] Re: Thank you all

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all. Thank you very much for reply to my query. This info is very
useful to know the current market.

Sincerely
V.Kabilan


==============================Original Headers==============================
3, 26 -- From velliyka-at-umdnj.edu Thu Jul 9 11:08:30 2009
3, 26 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126])
3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n69G8UYU002225
3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 11:08:30 -0500
3, 26 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id DA85810B875
3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 12:08:29 -0400 (EDT)
3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102])
3, 26 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 12FB310B7FE
3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 12:08:29 -0400 (EDT)
3, 26 -- Received: from ([10.32.15.171])
3, 26 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.19964033;
3, 26 -- Thu, 09 Jul 2009 12:08:25 -0400
3, 26 -- MIME-version: 1.0
3, 26 -- Content-transfer-encoding: 7BIT
3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed
3, 26 -- Received: from [10.4.62.229] ([10.32.15.102])
3, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built
3, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KMI00GIMVI0A610-at-umduwc02.umdnj.edu} for
3, 26 -- Microscopy-at-microscopy.com; Thu, 09 Jul 2009 12:08:25 -0400 (EDT)
3, 26 -- Message-id: {dd844f44945d5b9b5a1a1102bf86ed02-at-umdnj.edu}
3, 26 -- To: Microscopy-at-microscopy.com
3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu}
3, 26 -- Subject: Re: Thank you all
3, 26 -- Date: Thu, 09 Jul 2009 12:08:25 -0400
3, 26 -- X-Mailer: Apple Mail (2.624)
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 9 Jul 2009 11:13:17 -0500
Subject: [Microscopy] viaWWW: SEM image optimization - contrast &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

But why you are processing in 16 bit?

Vladimir

} -----Original Message-----
} From: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
} Sent: Thursday, July 09, 2009 9:03 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Re: viaWWW: SEM image optimization - contrast &
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I don't follow this set of guidelines. Adams' Zone system
} was based on expanding the tonal range of final prints.
} The first phase is The Negative while the second phase is The
} Print. Typically what I have done (in film era) is to over
} expose the neg and then under develop it. This significantly
} elongates the S-shape curve of the negative contrast range.
} In this situation, the middle part of the density area is
} elongated and results in more data for gray shades. Blown
} out highlights are useless as are under exposed black areas.
} The negative is first and foremost of importance. Typically
} over exposure of the neg by one stop and under development by
} 20% resulted in an excellent negative.
} Printing was then fairly routine.
}
} Why not opposite? Under expose the neg and over develop the print?
} If no light (data) on the film, it cannot be created by over
} developing. Once the exposure and development critera are
} set, Zone III is always the central target and that yields
} two zones below and above Zone III. Plus, all five zones are
} in a near perfect linear region.
}
} In a digital arena, my strategy is to process in 16-bits with
} either 12 or 14 native active bits. Input gamma adjustment
} is essential for creating the initial TIFF image. Most SEMs
} will do a contrast and brightness display with an upper line
} and lower line. With contrast adjusted to maximize peaks and
} valleys and brightness adjusted to keep all within the lines,
} that is a good starting point. Then look at the image. A
} black area is not going to show any info, hence, it needs
} more brightness. A white area also will show no info and
} thus, needs less contrast.
}
} A poor SEM image is easy to spot. Point and shoot does not apply.
} The eyes, hands and brain have to be working in sync to get a
} final crisp and useful photo/pix. Polaroids had limited
} tonal range and I see no pain about their loss.
}
} gary g.
}
} At 05:24 AM 7/9/2009, you wrote:
}
}
}
} } -------------------------------------------------------------
} ----------
} } ----- The Microscopy ListServer -- CoSponsor: The
} Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------
} } -----
} }
} } This Question/Comment was submitted to the Microscopy
} Listserver using
} } the WWW based Form at http://www.microscopy.com/MLFormMail.html
} } -------------------------------------------------------------
} ----------
} } ---- Remember this posting is most likely not from a Subscriber, so
} } when replying
} } please copy both apobanz-at-gmail.com as well as the
} MIcroscopy Listserver
} } -------------------------------------------------------------
} ----------
} } ----
} }
} } Email: apobanz-at-gmail.com
} } Name: Alan Pobanz
} }
} } Title-Subject: [Filtered] SEM image optimization - contrast &
} } brightness
} }
} } Question: Regarding adjusting contrast and brightness in SEM
} imaging,
} } Brian Tracy, in a microscopy seminar, gave an excellent description
} } about mimicking Ansel Adams B&W photos: 5% of the image should be
} } saturated black, 5% should be saturated white, and the
} remainder should
} } exhibit every possible gray level. I have a coworker that
} thinks this
} } is crazy (she uses very high contrast and no brightness).
} } Can you point me to some good reference material that describes how
} } best to adjust contrast and brightness in SEM images?
} } Thanks!
} } Alan
} }
} } Login Host: 63.163.107.100
} } -------------------------------------------------------------
} ----------
} } ----
} }
} } ==============================Original
} } Headers==============================
} } 5, 11 -- From zaluzec-at-microscopy.com Thu Jul 9 07:22:56
} 2009 5, 11 --
} } Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} } ESMTP id n69CMtrX000635
} } 5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009
} } 07:22:55 -0500
} } 5, 11 -- Mime-Version: 1.0
} } 5, 11 -- Message-Id: {p06240800c67b9177f95a-at-[206.69.208.22]}
} } 5, 11 -- Date: Thu, 9 Jul 2009 07:22:54 -0500 5, 11 -- To:
} } microscopy-at-microscopy.com 5, 11 -- From: apobanz-at-gmail.com
} (by way of
} } MicroscopyListserver) 5, 11 -- Subject: viaWWW: SEM image
} optimization
} } - contrast & brightness 5, 11 -- Content-Type: text/plain;
} } charset="us-ascii" ; format="flowed"
} } ==============================End of -
} } Headers==============================
}
}
} ==============================Original
} Headers==============================
} 10, 21 -- From gary-at-gaugler.com Thu Jul 9 09:02:19 2009 10,
} 21 -- Received: from smtp2.mc.surewest.net
} (qsmtp.mc.surewest.net [66.60.130.145])
} 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with SMTP id n69E2IkR024825
} 10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul
} 2009 09:02:18 -0500
} 10, 21 -- Message-Id: {200907091402.n69E2IkR024825-at-ns.microscopy.com}
} 10, 21 -- Received: (qmail 7427 invoked from network); 9 Jul
} 2009 06:51:45 -0700 10, 21 -- Received: by simscan 1.1.0
} ppid: 7424, pid: 7425, t: 0.0914s
} 10, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
} 10, 21 -- Received: from unknown (HELO thor.gaugler.com)
} (66.60.171.211)
} 10, 21 -- by smtp2 with SMTP; 9 Jul 2009 06:51:45 -0700
} 10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 10, 21 -- Date: Thu, 09 Jul 2009 07:02:09 -0700 10, 21 -- To:
} apobanz-at-gmail.com 10, 21 -- From: Gary Gaugler
} {gary-at-gaugler.com} 10, 21 -- Subject: Re: [Microscopy]
} viaWWW: SEM image optimization - contrast &
} 10, 21 -- brightness
} 10, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10,
} 21 -- In-Reply-To: {200907091224.n69COtWa005161-at-ns.microscopy.com}
} 10, 21 -- References: {200907091224.n69COtWa005161-at-ns.microscopy.com}
} 10, 21 -- Mime-Version: 1.0
} 10, 21 -- Content-Type: text/plain; charset="us-ascii";
} format=flowed ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
4, 25 -- From DusevichV-at-umkc.edu Thu Jul 9 11:13:17 2009
4, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n69GDFWi009503
4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 11:13:16 -0500
4, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Thu, 9 Jul 2009 11:13:13 -0500
4, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
4, 25 -- Content-class: urn:content-classes:message
4, 25 -- MIME-Version: 1.0
4, 25 -- Content-Type: text/plain;
4, 25 -- charset="US-ASCII"
4, 25 -- Subject: RE: [Microscopy] Re: viaWWW: SEM image optimization - contrast &
4, 25 -- Date: Thu, 9 Jul 2009 11:13:13 -0500
4, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB88A-at-KC-MSX1.kc.umkc.edu}
4, 25 -- In-Reply-To: {200907091402.n69E2nct026567-at-ns.microscopy.com}
4, 25 -- X-MS-Has-Attach:
4, 25 -- X-MS-TNEF-Correlator:
4, 25 -- Thread-Topic: [Microscopy] Re: viaWWW: SEM image optimization - contrast &
4, 25 -- thread-index: AcoAnfMR1twWFUyXQaeRy03tfIbFfQAEhd0Q
4, 25 -- References: {200907091402.n69E2nct026567-at-ns.microscopy.com}
4, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
4, 25 -- To: {Microscopy-at-microscopy.com}
4, 25 -- X-OriginalArrivalTime: 09 Jul 2009 16:13:13.0531 (UTC) FILETIME=[28FBB8B0:01CA00B0]
4, 25 -- Content-Transfer-Encoding: 8bit
4, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n69GDFWi009503
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 9 Jul 2009 13:17:37 -0500
Subject: [Microscopy] Acrolein Alternatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I'm asking this question for a colleague in Minnesota.

She has an investigator who wants to perfusion fix using acrolein. We know this is teargas and Homeland Security will probably give her the hairy eyeball if she buys it from EMS. Since we EM and histo types are already buying all sorts of seriously toxic nasties.

She needs to rapidly perfuse/fix a brain. What alternatives does she have?

Thanks in advance.

We're crying just thinking about using this stuff.

Paula :-)


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center (CRIC)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

Your images flow through our CRIC.




==============================Original Headers==============================
13, 20 -- From vapatpxs-at-yahoo.com Thu Jul 9 13:17:36 2009
13, 20 -- Received: from web46115.mail.sp1.yahoo.com (web46115.mail.sp1.yahoo.com [68.180.199.132])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n69IHY1P009191
13, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 9 Jul 2009 13:17:35 -0500
13, 20 -- Received: (qmail 69605 invoked by uid 60001); 9 Jul 2009 18:17:33 -0000
13, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1247163453; bh=z/cPk+++woviWn1d1MUi6+IQEiGRL0B276nwvXeuS7U=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=s9Jb50WdI+LupahW8IiVW8MJV4FPR/jTrPvYODrhBlHVnrASvoWNs2ygV2fOjEl4M7TnRioPvsB+xWfwlAmwj8DCySHpBtQXaFa9FIGoYiIiFv7o6ULX2ngHzJpIp1/lvtJlRn26OJFTTN3+wJqqaY5P/Ye+hqoqwr+v77/6xyo=
13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 20 -- s=s1024; d=yahoo.com;
13, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 20 -- b=IwWhoUr35SADZAPLuz6CXO6hXlfphTZQgWBgqm3czvP07PT1QrJpiG0eXvVvu9vBuOWH7vteQ0jvaOdyIVJklDfeOZSPfsWcVztK9p2Jz9NngbacRs81B0OfRNfM0mXIdHicfxR8ZhJhhQ8YMptrg77QCs4qtsmIkL/J9vQemzw=;
13, 20 -- Message-ID: {421947.68716.qm-at-web46115.mail.sp1.yahoo.com}
13, 20 -- X-YMail-OSG: UxnFnhoVM1ngwOf78KFbNYkt2wHG4PhaECUkU2e6Q.fiTDsoT0wngS_bgnD7Tv1SbniDrNVHwaC2KlNolg8Xp2YDJ_Da8BR7Q0_UFvl1Y802eAphAOsdTV.mYruj2VkJKpANGdBVLOH8slc60NvLloMzXsU7kiPxiwuFa80B.iRBil63IjKYeYOtOEzT_p0eCs8OFdW745wgzeQ.LJ__6zAAeqGTx3cBSjbTzSDRKQk4A_oQcOUeAw8nicPUEB3TZP8K5PQt2GNTNQX_qn9QUGccFYMNtrobIpa2Cg--
13, 20 -- Received: from [132.239.85.200] by web46115.mail.sp1.yahoo.com via HTTP; Thu, 09 Jul 2009 11:17:33 PDT
13, 20 -- X-Mailer: YahooMailClassic/5.4.17 YahooMailWebService/0.7.289.15
13, 20 -- Date: Thu, 9 Jul 2009 11:17:33 -0700 (PDT)
13, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 20 -- Subject: Acrolein Alternatives
13, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Thu, 9 Jul 2009 19:52:06 -0500
Subject: [Microscopy] SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

jacques.faerber-at-ipcms.u-strasbg.fr wrote:

}
} To Rick
}
} Thanks for your feedback. But as my english is not so good, I'm not
} sure to understand right your last sentence :
} } Because electronic noise adds in quadrature to the energy-dependent
} } component of resolution, a given resolution advantage at Mn-K
} } becomes a larger difference as a fraction of peak width for low
} } energies.
} Could you explain a little bit more ?


Sorry to answer a bit slowly, I've been away for a week
(in Alaska on a cruise ship with family). I figured one of
the usual suspects would have answered this already, but
here goes...

The resolution of an X-ray detector, commonly quoted as a single
number, varies with energy in reality. The resolution is much less
at low energies than at high energies.

A good approximation is: FWHM**2 = N**2 + cE

where FWHM is the fitted peak resolution, N is the "electronic
noise", c is a constant related to the so-called Fano factor,
and E is the energy in eV. 2.45 is a good value for 'c'.

So for example, a detector rated 129 eV at Mn K-alpha has
a noise resolution N of: N = sqrt(129**2 - (2.45*5898))
= 46.8 eV

Then you can compute the resolution at any other line energy,
such as O K-alpha: sqrt(46.8**2 + 2.45*525) = 59 eV

The proper value of 'c' for your detector can be determined
by measuring (very carefully) any two peaks and solving for
the value of c using the equation above.

Now make the Mn resolution a bit worse, say 138 eV. Then
N becomes 67.8 eV and O K-alpha becomes 76.7 eV, a relative
increase of (76.7/59) = 130% compared to (138/129) = 107%
for Mn K-alpha.

The noise resolution N is sometimes called the "zero peak"
resolution, or the resolution of a peak triggered in the
absence of any X-rays in the signal. It is determined by
the integration time of the pulse processor (variously
called "shaping time", "peaking time", "processing time"),
the SDD's capacitance, and the characteristics of the
first FET stage of the preamplifier. The capacitance
of an SDD is enormously lower than for a Si(Li), which
is one reason why it achieves its optimum resolution at
a much shorter integration time.

The lower the line energy, the greater the relative
contribution of N to the fitted peak resolution, until
at zero energy N is all that's left.

Rick Mott, PulseTor LLC


==============================Original Headers==============================
15, 18 -- From rmott-at-pulsetor.com Thu Jul 9 19:52:06 2009
15, 18 -- Received: from smtpout08.prod.mesa1.secureserver.net (smtpout08-01.prod.mesa1.secureserver.net [64.202.165.119])
15, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6A0q6X3015076
15, 18 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jul 2009 19:52:06 -0500
15, 18 -- Received: (qmail 13119 invoked from network); 10 Jul 2009 00:52:05 -0000
15, 18 -- Received: from unknown (98.221.243.51)
15, 18 -- by smtpout08.prod.mesa1.secureserver.net (64.202.165.119) with ESMTP; 10 Jul 2009 00:52:04 -0000
15, 18 -- Message-ID: {4A56908D.4010102-at-pulsetor.com}
15, 18 -- Date: Thu, 09 Jul 2009 20:51:25 -0400
15, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
15, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
15, 18 -- MIME-Version: 1.0
15, 18 -- To: microscopy-at-microscopy.com
15, 18 -- Subject: Re: [Microscopy] Re: SiLi v. SDD other quest.
15, 18 -- References: {200907010739.n617dF6K007679-at-ns.microscopy.com}
15, 18 -- In-Reply-To: {200907010739.n617dF6K007679-at-ns.microscopy.com}
15, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
15, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Fri, 10 Jul 2009 01:33:17 -0500
Subject: [Microscopy] Re: SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SDD resolution is more like a bathtub curve than
a straight line. If one keeps cps and DT in check,
the results are self-evident. I do not see how a
detector can do 1M cps and have any decent resolution
or low DT.

Show me how.

I'll put real data out in contrast.

gary g.


At 05:54 PM 7/9/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 20 -- From gary-at-gaugler.com Fri Jul 10 01:33:16 2009
10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6A6XGSL018604
10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 01:33:16 -0500
10, 20 -- Message-Id: {200907100633.n6A6XGSL018604-at-ns.microscopy.com}
10, 20 -- Received: (qmail 23085 invoked from network); 9 Jul 2009 23:22:39 -0700
10, 20 -- Received: by simscan 1.1.0 ppid: 23082, pid: 23083, t: 0.1254s
10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 20 -- by smtp2 with SMTP; 9 Jul 2009 23:22:39 -0700
10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 20 -- Date: Thu, 09 Jul 2009 23:33:06 -0700
10, 20 -- To: rmott-at-pulsetor.com
10, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 20 -- Subject: Re: [Microscopy] SiLi v. SDD other quest.
10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 20 -- In-Reply-To: {200907100054.n6A0s3Qr017463-at-ns.microscopy.com}
10, 20 -- References: {200907100054.n6A0s3Qr017463-at-ns.microscopy.com}
10, 20 -- Mime-Version: 1.0
10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gprodan-at-univ-ovidius.ro
Date: Fri, 10 Jul 2009 08:52:26 -0500
Subject: [Microscopy] viaWWW: RDF from SAED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gprodan-at-univ-ovidius.ro as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gprodan-at-univ-ovidius.ro
Name: gabi prodan

Organization: Ovidius University

Title-Subject: [Filtered] RDF from SAED

Question: Dear Listers,
RDF are very important to estimate structure of amorphous film. Can
we extract RDF from electron diffraction? I read some work related to
this, but I don't understand:

RDF are related to number of atoms (how can we estimate this number?)
In case of mounted specimen, it's possible to differentiate between
sample and support?

I try to use PDFFit and PDFGetX2(for XRD)but I'm still concerning how
to interpret the results, since the atomic factor are not the same
(for electron and X)

Thanks in advance
Gabi


Login Host: 81.196.74.130
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Fri Jul 10 08:52:26 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6ADqOqb007276
10, 11 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 08:52:25 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c67cf8000303-at-[206.69.208.22]}
10, 11 -- Date: Fri, 10 Jul 2009 08:52:24 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: gprodan-at-univ-ovidius.ro (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: RDF from SAED
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: obffn-at-yahoo.com
Date: Fri, 10 Jul 2009 08:52:58 -0500
Subject: [Microscopy] viaWWW: icamera replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both obffn-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: obffn-at-yahoo.com
Name: obffn

Organization: optical service engineer

Title-Subject: [Filtered] icamera replacement

Question: Iawant to replace 2/3"ccd camera in autoref topcon because
this is too much costly

Login Host: 58.27.152.126
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Jul 10 08:52:58 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6ADqtNF007888
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 08:52:58 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c67cf8220b02-at-[206.69.208.22]}
6, 11 -- Date: Fri, 10 Jul 2009 08:52:54 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: obffn-at-yahoo.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: icamera replacement
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Wadowska-at-upei.ca
Date: Fri, 10 Jul 2009 11:26:59 -0500
Subject: [Microscopy] H7000 Turbo molecular pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everybody,
Turbo molecular pump on our H7000 blew. We are looking for a replacement. There are a few options:
1. buy a rebuilt/refurbished turbo molecular pump
2. buy a new turbo molecular pump.
3. convert to a diffusion pump and install this pump instead of TM.
Has anybody of you faced this problem? What would you recommend?.
TIA
Dorota.

Dorota Wadowska
Manager
Electron Microscopy Laboratory
Atlantic Veterinary College,
UPEI
Charlottetown, PEI Canada
office 902 566 0849




==============================Original Headers==============================
5, 21 -- From Wadowska-at-grpwise.novell.upei.ca Fri Jul 10 11:26:58 2009
5, 21 -- Received: from mx2.upei.ca (magellanic.cs.upei.ca [137.149.3.22])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6AGQwMc018583
5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 11:26:58 -0500
5, 21 -- Received: from [137.149.64.174] (helo=grpwise.novell.upei.ca)
5, 21 -- by mx2.upei.ca with esmtp (Exim 4.50 #1 (Debian))
5, 21 -- id 1MPIw5-0001HV-0b
5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 13:26:57 -0300
5, 21 -- Received: from gwiadom-MTA by grpwise.novell.upei.ca
5, 21 -- with Novell_GroupWise; Fri, 10 Jul 2009 13:26:56 -0300
5, 21 -- Message-Id: {4A57419A.EA7E.0081.0-at-groupwise.upei.ca}
5, 21 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
5, 21 -- Date: Fri, 10 Jul 2009 13:26:51 -0300
5, 21 -- From: "Dorota Wadowska" {Wadowska-at-upei.ca}
5, 21 -- To: {Microscopy-at-microscopy.com}
5, 21 -- Subject: H7000 Turbo molecular pump
5, 21 -- Mime-Version: 1.0
5, 21 -- Content-Type: text/plain; charset=US-ASCII
5, 21 -- Content-Disposition: inline
5, 21 -- Content-Transfer-Encoding: 8bit
5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6AGQwMc018583
==============================End of - Headers==============================




From: Wadowska-at-upei.ca
Date: Fri, 10 Jul 2009 13:30:14 -0500
Subject: [Microscopy] turbomolecular pump H7000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
To follow e-mails that I received. Our pump is a "toast", the rotor inside looks like a ball so I think it is beyond repair. Pump spec are:
STP 300 H, S/N 0627.
TIA
Dorota

Dorota Wadowska
Manager
Electron Microscopy Laboratory
Atlantic Veterinary College,
UPEI
Charlottetown, PEI Canada
office 902 566 0849




==============================Original Headers==============================
5, 21 -- From Wadowska-at-grpwise.novell.upei.ca Fri Jul 10 13:30:14 2009
5, 21 -- Received: from mx2.upei.ca (magellanic.cs.upei.ca [137.149.3.22])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6AIUEIw010214
5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 13:30:14 -0500
5, 21 -- Received: from [137.149.64.174] (helo=grpwise.novell.upei.ca)
5, 21 -- by mx2.upei.ca with esmtp (Exim 4.50 #1 (Debian))
5, 21 -- id 1MPKrN-0003IV-MD
5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 15:30:13 -0300
5, 21 -- Received: from gwiadom-MTA by grpwise.novell.upei.ca
5, 21 -- with Novell_GroupWise; Fri, 10 Jul 2009 15:30:13 -0300
5, 21 -- Message-Id: {4A575E82.EA7E.0081.0-at-groupwise.upei.ca}
5, 21 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
5, 21 -- Date: Fri, 10 Jul 2009 15:30:10 -0300
5, 21 -- From: "Dorota Wadowska" {Wadowska-at-upei.ca}
5, 21 -- To: {Microscopy-at-microscopy.com}
5, 21 -- Subject: turbomolecular pump H7000
5, 21 -- Mime-Version: 1.0
5, 21 -- Content-Type: text/plain; charset=US-ASCII
5, 21 -- Content-Disposition: inline
5, 21 -- Content-Transfer-Encoding: 8bit
5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6AIUEIw010214
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Sun, 12 Jul 2009 10:05:04 -0500
Subject: [Microscopy] M&M Meeting proceedings offered to a good home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

This is to notify you that today morning, my computer or the internet
connection went a bit off the track and sent out an anonymous
invitation to you, claiming to be a facebook invitation. Please kindly
delete any such invitation received and do not accept. If I send an
invitation, usually will have a personalised message enclosed.

Regards,
Rachel

==============================Original Headers==============================
3, 118 -- From ecoagripolicy-at-gmail.com Fri Jul 10 23:26:34 2009
3, 118 -- Received: from mail-pz0-f200.google.com (mail-pz0-f200.google.com [209.85.222.200])
3, 118 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6B4QY79031314
3, 118 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 23:26:34 -0500
3, 118 -- Received: by pzk38 with SMTP id 38so284786pzk.10
3, 118 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jul 2009 21:26:33 -0700 (PDT)
3, 118 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 118 -- d=gmail.com; s=gamma;
3, 118 -- h=domainkey-signature:mime-version:received:date:message-id:subject
3, 118 -- :from:to:content-type:content-transfer-encoding;
3, 118 -- bh=gHF7Dq/uz+TlurrENLZnvMetJ0w9Vl4xquQJ+cJu9Rc=;
3, 118 -- b=MIbHBp8LG+Q8TaU08tU2iEh3OJ8VsRXspm+ur8ercNRJC976m/31d7a7xgwt3cyXob
3, 118 -- YHA5KNC8adK0E47O2paynhhbnAzbqJB9k0aecUgID6iMRPJgqMbLw4EvoBaB8zc1UQnX
3, 118 -- Tm+YInO7Ti5CcJcBqxVSXMKlpPCOaZqK32htQ=
3, 118 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 118 -- d=gmail.com; s=gamma;
3, 118 -- h=mime-version:date:message-id:subject:from:to:content-type
3, 118 -- :content-transfer-encoding;
3, 118 -- b=wOHodpFGlLx9x2rDX2mrAS7sBxCJ09/CpVLkSRQ3VoLhvhhkV+ap0DYisqPNkDm0yw
3, 118 -- U9aNTVHhad49D6vd+d7LW6cdeLQUTqH9POPWg9LJ7kLFhAvOxsFVOC+WpfKFXq1aTScF
3, 118 -- YZnoS08CtDKJYBBxrjFYI5Wiu3lwk5doUuA70=
3, 118 -- MIME-Version: 1.0
3, 118 -- Received: by 10.142.49.4 with SMTP id w4mr759451wfw.127.1247286390358; Fri, 10
3, 118 -- Jul 2009 21:26:30 -0700 (PDT)
3, 118 -- Date: Sat, 11 Jul 2009 09:56:30 +0530
3, 118 -- Message-ID: {ba6260910907102126t6e7caaebwda4c5ab1bca25487-at-mail.gmail.com}
3, 118 -- Subject: re:Apologies
3, 118 -- From: Rachel Predeepa {ecoagripolicy-at-gmail.com}
3, 118 -- To: abiramiu {abiramiU-at-gmail.com} , administration-at-phddata.org,
3, 118 -- alan-at-fnas.uwa.edu.au, Alicia Zablah {alicia.zablah-at-uwa.edu.au} ,
3, 118 -- Amit Malhotra {amit.malhotra-at-idp.com} ,
3, 118 -- Biotech-Mod4 {biotech-mod4-at-fao.org} , biotech-room4-at-mailserv.fao.org,
3, 118 -- boda-at-atmail.com, botanycontent-at-gmail.com,
3, 118 -- Carmel Ewing {Carmel.Ewing-at-lwa.gov.au} , cchaitanya-at-gmail.com,
3, 118 -- Christine Kerin {chris.kerin-at-uwa.edu.au} ,
3, 118 -- =?UTF-8?B?w4fDjGrDtSDGklLDok7CouKAocWg?= {cijo321-at-gmail.com} ,
3, 118 -- consumercentral-at-acresso.com, cop-citynews-request-at-lists.highway1.biz,
3, 118 -- cop-pubnoticeboard-request-at-lists.highway1.biz,
3, 118 -- COPUSnews-l administration {COPUSnews-l-subscribe-at-aibs.org} ,
3, 118 -- cordovaeditors-at-hotmail.com, Dana Crisan {admin.cmca-at-uwa.edu.au} ,
3, 118 -- david_gri-at-rediffmail.com, david.glance-at-csp.uwa.edu.au, david-at-yahoo.com,
3, 118 -- davorka-at-fnas.uwa.edu.au, Deborah Pyatt {deborah.pyatt-at-uwa.edu.au} ,
3, 118 -- Deepu Thomas John {deeputhomas80-at-gmail.com} , dhanya.renjen-at-gmail.com,
3, 118 -- diby {diby007-at-gmail.com} , dickreisz-at-frontiernet.net,
3, 118 -- "DILIP JAMES!!!" {james.dilip-at-gmail.com} , ecoraji_ias-at-gmail.com,
3, 118 -- ecoraji-at-gmail.com, ed.selvakumar-at-gmail.com, ed.sevakumar-at-gmail.com,
3, 118 -- editor-at-atlanticbooks.com, editorial-at-atlanticbooks.com,
3, 118 -- enquiries-at-guild.uwa.edu.au, entdr-at-hotmail.com, events-at-curtin.edu.au,
3, 118 -- firoz pm {firozpm-at-gmail.com} , gayathri-at-yahoo.com,
3, 118 -- gerald.koch-at-vti.bund.de, gkoch-at-holz.uni-hamburg.de, govind-at-yahoo.com,
3, 118 -- gronbek-at-gronbeksecurity.com.au, harish {harish.dr.nagpal-at-gmail.com} ,
3, 118 -- harmeet.pental-at-idp.com, harmit.pental-at-idp.com,
3, 118 -- "home alone :(" {satishkevin-at-gmail.com} ,
3, 118 -- Housing {housing-at-admin.uwa.edu.au} , housing-at-uwa.edu.au,
3, 118 -- hr-at-wwfindia.net, iivanova-at-hatch.com.au, ijmami-at-gmail.com,
3, 118 -- info-at-peoplecar.com.au,
3, 118 -- international-sc.1198149087.pnhnlkoppiiffladapfm-ecoagripolicy=gmail.com-at-lists.science.org.au,
3, 118 -- international-sc.1198979393.iffjoheojdipkkcomjkc-ecoagripolicy=gmail.com-at-lists.science.org.au,
3, 118 -- ithelp-its-at-uwa.edu.au, ithelp-itshelp-at-uwa.edu.au,
3, 118 -- its-help-at-admin.uwa.edu.au, IVORY RS {fegdasky-at-gmail.com} ,
3, 118 -- Jacqueline Gilchrist {reception-at-guild.uwa.edu.au} ,
3, 118 -- Jessica Roberts {jessicaroberts-at-anxa.com} ,
3, 118 -- Jessica Roberts {noreply-us-at-anxa.com} ,
3, 118 -- jigna modh {jigna.modh-at-gmail.com} , Jiju Alex {jijualexk-at-gmail.com} ,
3, 118 -- jms-at-uwa.edu.au, Jo Hocking {Jo.Hocking-at-uwa.edu.au} ,
3, 118 -- khaisp01-at-student.uwa.edu.au, kontakt-at-systat.de,
3, 118 -- kvmallaiah-at-rediffmail.com, kyocera-at-uwa.edu.au,
3, 118 -- lhodgson-at-cyllene.uwa.edu.au, likia-at-yahoo.com,
3, 118 -- Lucy Reilly {lucy.reilly-at-uwa.edu.au} , madhu {biozeal-at-gmail.com} ,
3, 118 -- mailserv-at-mailserv.fao.org, Maja Milosevic {pb-purch-at-plants.uwa.edu.au} ,
3, 118 -- malabika {malabika.maulik-at-gmail.com} , manas_jhakaas-at-rediffmail.com,
3, 118 -- Marut Agarwal {eugene.enigmatic-at-gmail.com} ,
3, 118 -- megha {megs.saha-at-gmail.com} , Michelle_Yong-at-dell.com,
3, 118 -- microscopy-at-microscopy.com, midhun charls {midhuncharls-at-gmail.com} ,
3, 118 -- milydevji {milydevji-at-gmail.com} ,
3, 118 -- Miroslav Buran {miroslav.buran-at-upek.com} , mludwig-at-cyllene.uwa.edu.au,
3, 118 -- Nadia Bazihizina {bazihn01-at-student.uwa.edu.au} ,
3, 118 -- Nagib Nassar {nagnassa-at-rudah.com.br} ,
3, 118 -- newtoperth {newtoperth-at-yahoo.com} , online-at-westpac.com.au,
3, 118 -- Peter Matthews {pjm-at-gol.com} ,
3, 118 -- Plant Biology Admin {plantbio-at-plants.uwa.edu.au} , pratibha.sen-at-idp.com,
3, 118 -- preveenaacottons108-at-gmail.com, rachel8predeeba-at-yahoo.com,
3, 118 -- rachelpredeepa-at-hotmail.com, Radhika {radhika-at-idp.com} ,
3, 118 -- Ram {ramjnu-at-gmail.com} , ranjith-at-yahoo.com, registrar-at-mail.jnu.ac.in,
3, 118 -- registtrar-at-mail.jnu.ac.in, rekha {rekharekha123-at-rediffmail.com} ,
3, 118 -- Renu Sharma {rsharma-at-fnas.uwa.edu.au} , Renu_Chauhan-at-dell.com,
3, 118 -- riyaz-at-yahoo.com, Rosemary White {rosemary.white-at-csiro.au} ,
3, 118 -- rpredeepa-at-ymail.com, Rqachel Predeepa {ecoagripolicy-at-gmail.com} ,
3, 118 -- rtotn57-at-tn.nic.in, saifulhc {saifulhc-at-yahoo.com} ,
3, 118 -- Sanjeev Philip Thomas {sanjeev_forever-at-hotmail.com} ,
3, 118 -- santhosh cha {santhosh.cherian-at-gmail.com} ,
3, 118 -- sarika {tyagisarika1402-at-gmail.com} ,
3, 118 -- satish {microbiologistkevin-at-gmail.com} , sbavich-at-admin.uwa.edu.au,
3, 118 -- shafi {shafiunme-at-gmail.com} , shanu santhosh {shanu.santhosh-at-gmail.com} ,
3, 118 -- Shashi Rekha S C {shashirsc-at-gmail.com} ,
3, 118 -- Sid Mamella {sridhar.mamella-at-gmail.com} ,
3, 118 -- Smarte XS {smartexs-at-smartecarte.com.au} ,
3, 118 -- Sonia Thomas {itssony80-at-gmail.com} ,
3, 118 -- Sony Samuel {samuel.sony-at-gmail.com} , sridevi_microbio-at-yahoo.co.in,
3, 118 -- Sudhir Sahadev {sudhir04-at-gmail.com} , support.student-at-uwa.edu.au,
3, 118 -- support-at-student.uwa.edu.au, Suraj Thapa {t.suraj-at-ymail.com} ,
3, 118 -- swarup {swarup25mitra-at-gmail.com} ,
3, 118 -- Tamilarasi Muthukumar {tamilmk77-at-yahoo.co.in} , tamilmk-at-yahoo.co.in,
3, 118 -- "Tan, Aik Loon" {AikLoon.Tan-at-auspost.com.au} , tanvi.jain-at-airtel.in,
3, 118 -- tdcolmer-at-cyllen.uwa.edu.au, tdcolmer-at-cyllene.uwa.edu.au,
3, 118 -- tdcolmer-at-studnt.uwa.edu.au,
3, 118 -- The BigPond Team {thebigpondteam-at-bigpond-mail.custhelp.com} ,
3, 118 -- venu-at-yahoo.com,
3, 118 -- Virgin Mobile Customer Service {team-at-virginmobile.com.au} ,
3, 118 -- vishal-at-yahoo.com, vishy2003-at-gmail.com, vkj0400-at-hotmail.com,
3, 118 -- vodafonecare.del-at-vodafone.com, vodafonecare.del-at-vodafone.in,
3, 118 -- vu3ybm {vu3ybm-at-yahoo.com} , vyk-at-vykonline.org,
3, 118 -- WA Cust Care {WACustCare-at-auspost.com.au} ,
3, 118 -- Wolfgang Muss {W.Muss-at-salk.at} ,
3, 118 -- ybalamuralikrishna {ybalamuralikrishna-at-gmail.com} ,
3, 118 -- yhostel-at-del2.vsnl.net.in
3, 118 -- Content-Type: text/plain; charset=ISO-8859-1
3, 118 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From jyxjfrpc-at-public1.wx.js.cn Sat Jul 11 20:50:07 2009
Return-Path: {jyxjfrpc-at-public1.wx.js.cn}
Received: from jlonline.com (pip32.ptt.js.cn [61.155.13.208])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6C1ndBp017232;
Sat, 11 Jul 2009 20:49:52 -0500
Received: from User([196.47.83.62]) by ptt.js.cn(AIMC 3.2.0.0)
with SMTP id jm1324a599d16; Sun, 12 Jul 2009 09:37:48 +0800
Reply-To: {williamwilcox09-at-sify.com}

I have printed M&M proceedings for 2000 and 2002 to 2006 that I no
longer need.

I'll be happy to ship them as a group to a new home in the USA at my
expense, or outside the USA at your expense.

First come, first served.

Ron Anderson


==============================Original Headers==============================
5, 17 -- From randerson20-at-tampabay.rr.com Sun Jul 12 10:05:03 2009
5, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6CF53Fr019068
5, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 12 Jul 2009 10:05:03 -0500
5, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
5, 17 -- by hrndva-omta04.mail.rr.com with ESMTP
5, 17 -- id {20090712150500228.YMRN16393-at-hrndva-omta04.mail.rr.com}
5, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 12 Jul 2009 15:05:00 +0000
5, 17 -- Message-ID: {4A59FB7E.5010400-at-tampabay.rr.com}
5, 17 -- Date: Sun, 12 Jul 2009 11:04:30 -0400
5, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
5, 17 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
5, 17 -- MIME-Version: 1.0
5, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
5, 17 -- Subject: M&M Meeting proceedings offered to a good home
5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 17 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: randerson20-at-tampabay.rr.com
Date: Sun, 12 Jul 2009 11:17:23 -0500
Subject: [Microscopy] M&M Meeting proceedings found a good home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The proceedings have been claimed. A graduate student in the Midwest USA.

Ron


==============================Original Headers==============================
3, 17 -- From randerson20-at-tampabay.rr.com Sun Jul 12 11:17:23 2009
3, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122])
3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6CGHMuF003749
3, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 12 Jul 2009 11:17:23 -0500
3, 17 -- Received: from [127.0.0.1] (really [24.73.73.214])
3, 17 -- by hrndva-omta04.mail.rr.com with ESMTP
3, 17 -- id {20090712161722357.ZZGD16393-at-hrndva-omta04.mail.rr.com}
3, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 12 Jul 2009 16:17:22 +0000
3, 17 -- Message-ID: {4A5A0C75.8010102-at-tampabay.rr.com}
3, 17 -- Date: Sun, 12 Jul 2009 12:16:53 -0400
3, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com}
3, 17 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
3, 17 -- MIME-Version: 1.0
3, 17 -- To: Listserver {Microscopy-at-Microscopy.Com}
3, 17 -- Subject: M&M Meeting proceedings found a good home
3, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 17 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: rmott-at-pulsetor.com
Date: Sun, 12 Jul 2009 13:13:51 -0500
Subject: [Microscopy] Re: SiLi v. SDD other quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary Gaugler wrote:
} SDD resolution is more like a bathtub curve than
} a straight line. If one keeps cps and DT in check,
} the results are self-evident. I do not see how a
} detector can do 1M cps and have any decent resolution
} or low DT.
}

It can't, of course, if you mean maintaining anything
near "best" resolution. The noise component of the
resolution equation includes the effect of DPP
integration time. If that is very short (as it must
be at very high rates), N will be larger and resolution
will be worse than for "optimum" integration time.

The relationship between total desd time per pulse,
including all integration intervals and any "gap" or
"flat top" in the filter function, and the maximum
throughput is approximated by:

max throughput = 1 / (2.7D)

where D is the total dead time per pulse. You can
turn that around to get the required D for some
desired throughput OCR:

D = 1 / (2.7 times OCR)


However, there are differences between SDDs in terms
of their resolution as a function of shaping time.
Integrated FET devices deliver better resolution at
shorter integration times, resulting in higher throughput
for a given resolution (smaller D in the above equations).

How much of this is due to net capacitance differences and
how much is due to differences in FET noise characteristics,
I don't know enough to tell you.

Rick Mott, PulseTor LLC




==============================Original Headers==============================
13, 18 -- From rmott-at-pulsetor.com Sun Jul 12 13:13:51 2009
13, 18 -- Received: from smtpauth11.prod.mesa1.secureserver.net (smtpauth11.prod.mesa1.secureserver.net [64.202.165.33])
13, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6CIDpGh022016
13, 18 -- for {Microscopy-at-Microscopy.Com} ; Sun, 12 Jul 2009 13:13:51 -0500
13, 18 -- Received: (qmail 13083 invoked from network); 12 Jul 2009 18:13:50 -0000
13, 18 -- Received: from unknown (24.59.153.241)
13, 18 -- by smtpauth11.prod.mesa1.secureserver.net (64.202.165.33) with ESMTP; 12 Jul 2009 18:13:50 -0000
13, 18 -- Message-ID: {4A5A27DE.9090302-at-pulsetor.com}
13, 18 -- Date: Sun, 12 Jul 2009 14:13:50 -0400
13, 18 -- From: Rick Mott {rmott-at-pulsetor.com}
13, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
13, 18 -- MIME-Version: 1.0
13, 18 -- To: Microscopy-at-Microscopy.Com
13, 18 -- Subject: Re: [Microscopy] SiLi v. SDD other quest.
13, 18 -- References: {200907100054.n6A0s3Qr017463-at-ns.microscopy.com}
13, 18 -- In-Reply-To: {200907100054.n6A0s3Qr017463-at-ns.microscopy.com}
13, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
13, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: s.k.pountney-at-reading.ac.uk
Date: Mon, 13 Jul 2009 08:14:29 -0500
Subject: [Microscopy] viaWWW: Honey crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both s.k.pountney-at-reading.ac.uk as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: s.k.pountney-at-reading.ac.uk
Name: Stephen Pountney

Organization: University

Title-Subject: [Filtered] Honey crystals

Question: I am trying to image and monitor sugar crytal formation in
clear honey over time following innoculation with 'honey seed
solution', ideally to see 3D structures. Any advice gratefully
received.

Login Host: 134.225.154.67
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Jul 13 08:14:29 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6DDERgf007244
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 08:14:28 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c680e3a18a58-at-[206.69.208.22]}
6, 11 -- Date: Mon, 13 Jul 2009 08:14:26 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: s.k.pountney-at-reading.ac.uk (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Honey crystals
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 13 Jul 2009 12:48:13 -0500
Subject: [Microscopy] LaB6 emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

I'm interested in hearing from people who have used third-party LaB6
emitters vs. OEM products. I am particularly curious about Kimball
Physics' line of emitters, in terms of stability, compatibility, useful
life, etc, but am open to thoughts on all vendors' products. We will
soon be replacing the emitter on our JEOL JEM 1400 TEM, which we use for
general TEM work but occasionally is called upon for high-mag work with
eensy nanoparticles.

BTW, this does not imply any dissatisfaction with our current emitter or
JEOL's service in any way, shape or form---on the contrary, actually.
I'm just curious how the various providers stack up.

Any and all replies welcome, including vendors, of course. Off list is
fine, too.

Thanks in advance.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 27 -- From TindallR-at-missouri.edu Mon Jul 13 12:48:13 2009
10, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6DHmCk4005066
10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 12:48:13 -0500
10, 27 -- X-IronPort-Anti-Spam-Filtered: true
10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAGsQW0rRauUo/2dsb2JhbADBVwEJhWCIT4JNgTwFgT0
10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
10, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 13 Jul 2009 12:48:12 -0500
10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 27 -- Mon, 13 Jul 2009 12:48:12 -0500
10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 27 -- Content-class: urn:content-classes:message
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Type: text/plain;
10, 27 -- charset="us-ascii"
10, 27 -- Subject: LaB6 emitters
10, 27 -- Date: Mon, 13 Jul 2009 12:48:12 -0500
10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD822F-at-UM-XMAIL08.um.umsystem.edu}
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- Thread-Topic: LaB6 emitters
10, 27 -- Thread-Index: AcoD4hc9JhRw+5heS7KinqPhOmk2iQ==
10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 27 -- To: {microscopy-at-microscopy.com}
10, 27 -- X-OriginalArrivalTime: 13 Jul 2009 17:48:12.0599 (UTC) FILETIME=[178B7C70:01CA03E2]
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6DHmCk4005066
==============================End of - Headers==============================




From: dhorne-at-interchange.ubc.ca
Date: Mon, 13 Jul 2009 17:57:23 -0500
Subject: [Microscopy] viaWWW: out of focus raster on S4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dhorne-at-interchange.ubc.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] out of focus raster on S4700

Question: Our S4700 demonstrated a new problem the other day.

During an image acquisition in line capture mode, there were
alternating bands that were in and out of focus along the x axis.
This could not be explained by anything to do with the sample (i.e.
regular, repeating, large-magnitude topographical changes), and was
occurring at all mags that we checked. I believe they were there
with varying degrees of raster rotation engaged as well. I did not
measure the spacing, but it did look as if the distortion was
repeating at regular intervals.

After realigning the scope, noting there was some charging at
spacings in the cuticle at the edges of the image, and changing to a
different objective aperture, I was able to minimize but not
eliminate the problem. It did not reappear at startup this week.

Can anyone offer an explanation to how this distortion could be
caused? I'm particularly interested in the electronics side of it, as
this has been our bugbear over the last few years.

Thanks in advance

Derrick

Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Mon Jul 13 17:57:22 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6DMvL9Q002566
11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 17:57:22 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240800c6816c3c8f4d-at-[206.69.208.22]}
11, 11 -- Date: Mon, 13 Jul 2009 17:57:21 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: out of focus raster on S4700
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Mon, 13 Jul 2009 21:45:25 -0500
Subject: [Microscopy] AMS carbon 14 dating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

I'm looking for a lab that can perform AMS carbon dating. If anyone knows
or can recommend a lab with this capability please let me know their
contact info if possible.

Thank you very much,

dj





==============================Original Headers==============================
8, 20 -- From dljones-at-bestweb.net Mon Jul 13 21:45:24 2009
8, 20 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6E2jOVc031690
8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 21:45:24 -0500
8, 20 -- Received: from Pappee (ool-435170eb.dyn.optonline.net [67.81.112.235])
8, 20 -- by mta2.srv.hcvlny.cv.net
8, 20 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007))
8, 20 -- with ESMTP id {0KMR00GP63NOKZF0-at-mta2.srv.hcvlny.cv.net} for
8, 20 -- Microscopy-at-microscopy.com; Mon, 13 Jul 2009 22:45:24 -0400 (EDT)
8, 20 -- Date: Mon, 13 Jul 2009 22:45:26 -0400 (Eastern Daylight Time)
8, 20 -- From: "David L. Jones" {dljones-at-bestweb.net}
8, 20 -- Subject: AMS carbon 14 dating
8, 20 -- In-reply-to: {200907091554.n69Fsrnc024647-at-ns.microscopy.com}
8, 20 -- X-X-Sender: dljones-at-imap.bestweb.net
8, 20 -- To: Microscopy-at-microscopy.com
8, 20 -- Message-id: {Pine.WNT.4.64.0907132235150.4696-at-Pappee}
8, 20 -- MIME-version: 1.0
8, 20 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed
8, 20 -- Content-transfer-encoding: 7BIT
8, 20 -- References: {200907091554.n69Fsrnc024647-at-ns.microscopy.com}
==============================End of - Headers==============================




From: jtwilley-at-sprynet.com
Date: Mon, 13 Jul 2009 21:57:49 -0500
Subject: [Microscopy] Re: AMS carbon 14 dating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

DJ,

You have not indicated what the material is that you wish to have dated or how old it may possibly be. There are a lot of considerations involved, including the removal of contamination based on its prior circumstances and condition, and the class of material to be dated. I have a good deal of experience with this and would advise you based on the type of material. Some accelerator AMS facilities have staff members with particular experience in the application of appropriate offsets when converting the raw radiocarbon measurement to a calendar date. This is especially true when dealing with marine material. In my experience, some have been more responsive than others to the demands of specific pretreatment steps.

Depending on the object and material, there may be important considerations in where the sample should be taken so as to best answer the underlying question.

John Twilley
Conservation Scientist
P.O. Box 215
Hawthorne, NY 10532
USA

-----Original Message-----
} From: dljones-at-bestweb.net
} Sent: Jul 13, 2009 10:45 PM
} To: jtwilley-at-sprynet.com
} Subject: [Microscopy] AMS carbon 14 dating
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
6, 25 -- From jtwilley-at-sprynet.com Mon Jul 13 21:57:49 2009
6, 25 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6E2vn3R013747
6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 21:57:49 -0500
6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 25 -- s=dk20050327; d=sprynet.com;
6, 25 -- b=eZaBKQm2aOPE2xjwkg4cy4oWiqa6ef8EJyHDHfPYmHHM3y6BjlJaNreqRIi2d9No;
6, 25 -- h=Message-ID:Date:From:Reply-To:To:Subject:Mime-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-ELNK-Trace:X-Originating-IP;
6, 25 -- Received: from [209.86.224.28] (helo=mswamui-blood.atl.sa.earthlink.net)
6, 25 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
6, 25 -- (envelope-from {jtwilley-at-sprynet.com} )
6, 25 -- id 1MQYDE-0002JY-NU; Mon, 13 Jul 2009 22:57:48 -0400
6, 25 -- Received: from 70.23.75.247 by webmail.earthlink.net with HTTP; Mon, 13 Jul 2009 22:57:48 -0400
6, 25 -- Message-ID: {18002091.1247540268720.JavaMail.root-at-mswamui-blood.atl.sa.earthlink.net}
6, 25 -- Date: Mon, 13 Jul 2009 22:57:48 -0400 (EDT)
6, 25 -- From: jtwilley-at-sprynet.com
6, 25 -- Reply-To: jtwilley-at-sprynet.com
6, 25 -- To: dljones-at-bestweb.net, Microscopy-at-microscopy.com
6, 25 -- Subject: Re: [Microscopy] AMS carbon 14 dating
6, 25 -- Mime-Version: 1.0
6, 25 -- Content-Type: text/plain; charset=UTF-8
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- X-Mailer: EarthLink Zoo Mail 1.0
6, 25 -- X-ELNK-Trace: adbb89a220ca650b5741bb2dafe8270584f945684cbf6968fb03a1e520c9e1f61c73108cdc4eecf7350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
6, 25 -- X-Originating-IP: 209.86.224.28
==============================End of - Headers==============================




From: jdsugar-at-sandia.gov
Date: Tue, 14 Jul 2009 05:31:55 -0500
Subject: [Microscopy] viaWWW: Tabbed Text Importer for Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jdsugar-at-sandia.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jdsugar-at-sandia.gov
Name: Josh Sugar

Organization: Sandia

Title-Subject: [Filtered] Tabbed Text Importer for Digital Micrograph

Question: Does anyone have a script that will import tabbed text into
Digital Micrograph as a spectrum? I have some spectra from our Oxford
EDS system that I would like to analyze in DM.

Thanks,
Josh

Login Host: 198.206.219.44
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Jul 14 05:31:53 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EAVqxB031928
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 05:31:52 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c6820f06b772-at-[206.69.208.22]}
7, 11 -- Date: Tue, 14 Jul 2009 05:31:51 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: jdsugar-at-sandia.gov (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Tabbed Text Importer for Digital Micrograph
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mary.walker-at-csl.com.au
Date: Tue, 14 Jul 2009 05:32:20 -0500
Subject: [Microscopy] viaWWW: SEM Preparation of Lyophilized/Freeze-Dried Product

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mary.walker-at-csl.com.au as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: mary.walker-at-csl.com.au
Name: mary walker

Organization: CSL Limited

Title-Subject: [Filtered] SEM Preparation of Lyophilized/Freeze-Dried Product

Question: I am interested in observing the surface structure of a
lyophilized cake using SEM. I am having difficulties with some of
the samples, they will not stick to the carbon tape. Any suggestions

Thanks in advance

Mary

Login Host: 203.10.36.68
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Jul 14 05:32:20 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EAWJZI032127
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 05:32:20 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6820f21bdcc-at-[206.69.208.22]}
8, 11 -- Date: Tue, 14 Jul 2009 05:32:19 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mary.walker-at-csl.com.au (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: SEM Preparation of Lyophilized/Freeze-Dried Product
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Tue, 14 Jul 2009 07:42:46 -0500
Subject: [Microscopy] Oxford Inca (ISIS) system error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning listers,

Lately I've been getting the following error message on startup of our
Oxford Inca 200 system:

Isis System Error
Isis Link Driver:
fault (Isis hardware)
Error 85,[0] 15.54V Analog 15V above limit {00,00}

So far this message has only appeared on startup and when the system is
idle - doesn't seem to crop up when I'm actually working with the
system, and seems to show up less frequently with time after the
software is started. Also so far the "measured" over voltage is always
exactly 15.54V, so my thinking is that it is some digital value that is
just one bit off acceptable. I can dismiss the error and everything
seems to be working normally.

Any ideas out there? Livable nuisance? Easy no-brainer fix? Omen of
impending doom? The system is coming up on 10 years old, but we really
don't have the use to justify (or the desire) scrounging for funds to
upgrade the system, so anything I can do to keep it breathing would be
worthwhile.

As always, thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
11, 18 -- From jehrman-at-mta.ca Tue Jul 14 07:42:46 2009
11, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.201])
11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6ECgkO8010991
11, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 07:42:46 -0500
11, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:51202)
11, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
11, 18 -- with esmtp id 1MQhOr-0007hq-Or (Exim 4.69) for Microscopy-at-microscopy.com
11, 18 -- (return-path {jehrman-at-mta.ca} ); Tue, 14 Jul 2009 09:46:25 -0300
11, 18 -- Message-ID: {4A5C7BDE.3000409-at-mta.ca}
11, 18 -- Date: Tue, 14 Jul 2009 09:36:46 -0300
11, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
11, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
11, 18 -- MIME-Version: 1.0
11, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
11, 18 -- Subject: Oxford Inca (ISIS) system error
11, 18 -- X-Enigmail-Version: 0.95.7
11, 18 -- Content-Type: text/plain; charset=ISO-8859-1
11, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 14 Jul 2009 08:43:28 -0500
Subject: [Microscopy] Mystery course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've got a small mystery I hope the microscopy community can help solve.
My lab has what looks like professional bound course notes called "Electron
Microscopy in Failure Analysis" but there is no information on when it was
published, for what course, or where the course was offered.

It's an older text, The EDS spectra reminds me of the silver thermal paper
used in the late 70's. Two pages have the letterhead from SEAL, Scanning
Electron Analysis Laboratories, Inc located at 5301 Beethoven St. Los
Angeles, but no date.

An article entitled " Use of Laboratory Failure Simulation Exemplars to
Study Intergranular Fracture modes in 9Ni-4Co-0.20C Steel" by Young and
Kumar. ASTM published it in 1978.,

The only other historical date is a photo essay on "CH-53 crash on I-80".

So.... does any of this ring a bell, jog a memory, make anyone remember
some amusing incident from this course? If so help me put a date and
course information on this.

Thanks!
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
9, 21 -- From frank_karl-at-lincolnelectric.com Tue Jul 14 08:43:28 2009
9, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EDhSOr029192
9, 21 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 08:43:28 -0500
9, 21 -- Subject: Mystery course
9, 21 -- To: Microscopy-at-microscopy.com
9, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
9, 21 -- Message-ID: {OF8F96712B.62D3DE8E-ON852575F3.0048FF19-852575F3.004B5BB5-at-lincolnelectric.com}
9, 21 -- Date: Tue, 14 Jul 2009 09:43:06 -0400
9, 21 -- From: Frank_Karl-at-lincolnelectric.com
9, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 07/14/2009 09:43:05 AM,
9, 21 -- CD-MIME complete at 07/14/2009 09:43:05 AM,
9, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 07/14/2009 09:43:05 AM,
9, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
9, 21 -- 07, 2008) at 07/14/2009 09:43:05 AM,
9, 21 -- Serialize complete at 07/14/2009 09:43:05 AM
9, 21 -- MIME-Version: 1.0
9, 21 -- Content-Type: text/plain;
9, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Tue, 14 Jul 2009 09:34:57 -0500
Subject: [Microscopy] Oxford Inca (ISIS) system error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning, Jim.

We have a venerable ISIS system here that we are using. Personally, I
like the ISIS interface better than the INCA interface. One of these
days I will have to do something about it, but not just yet.

A couple years back, we encountered similar errors indicating that one
or more of our voltages were out of spec. There are test points behind
the front panel that you could check with a voltmeter. There is also a
validation utility program as part of the ISIS package that should let
you check the voltages at will. I would have to check my notes for the
details on using it.

Anyway, we confirmed that our power supply was failing. Oxford could
supply a replacement, but it would cost quite a bit. Instead, I put the
word out on this list figuring there must be some ISIS units taken out
of service and gathering dust somewhere or another. Indeed there were. I
got two replies about two such systems and secured one and had it
shipped to me. (Thanks, Scott.) It was a fairly simple matter to swap
out the power supply module and we are still running with that module.

I don't know if the second unit is still out there, but I could dig up
the info. Probably there are other units that have been retired since I
checked. This community is gracious enough that you could probably
secure one without too much trouble.

Warren

-----Original Message-----
X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca]
Sent: Tuesday, July 14, 2009 7:44 AM
To: wesaia-at-iastate.edu

Good morning listers,

Lately I've been getting the following error message on startup of our
Oxford Inca 200 system:

Isis System Error
Isis Link Driver:
fault (Isis hardware)
Error 85,[0] 15.54V Analog 15V above limit {00,00}

So far this message has only appeared on startup and when the system is
idle - doesn't seem to crop up when I'm actually working with the
system, and seems to show up less frequently with time after the
software is started. Also so far the "measured" over voltage is always
exactly 15.54V, so my thinking is that it is some digital value that is
just one bit off acceptable. I can dismiss the error and everything
seems to be working normally.

Any ideas out there? Livable nuisance? Easy no-brainer fix? Omen of
impending doom? The system is coming up on 10 years old, but we really
don't have the use to justify (or the desire) scrounging for funds to
upgrade the system, so anything I can do to keep it breathing would be
worthwhile.

As always, thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



==============================Original Headers==============================
20, 37 -- From wesaia-at-iastate.edu Tue Jul 14 09:34:57 2009
20, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
20, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EEYv9g014526
20, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 09:34:57 -0500
20, 37 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
20, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n6EEYuU6022808;
20, 37 -- Tue, 14 Jul 2009 09:34:56 -0500
20, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
20, 37 -- id 3dea_808fab98_7083_11de_bc91_001372578af6;
20, 37 -- Tue, 14 Jul 2009 09:34:56 -0500
20, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
20, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n6EEYuYW007814;
20, 37 -- Tue, 14 Jul 2009 09:34:56 -0500
20, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
20, 37 -- Tue, 14 Jul 2009 09:34:56 -0500
20, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 37 -- Content-class: urn:content-classes:message
20, 37 -- MIME-Version: 1.0
20, 37 -- Content-Type: text/plain;
20, 37 -- charset="us-ascii"
20, 37 -- Subject: RE: [Microscopy] Oxford Inca (ISIS) system error
20, 37 -- Date: Tue, 14 Jul 2009 09:36:53 -0500
20, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703FFF362-at-maire.eng.iastate.edu}
20, 37 -- In-Reply-To: {200907141243.n6EChd4E012051-at-ns.microscopy.com}
20, 37 -- X-MS-Has-Attach:
20, 37 -- X-MS-TNEF-Correlator:
20, 37 -- Thread-Topic: [Microscopy] Oxford Inca (ISIS) system error
20, 37 -- Thread-Index: AcoEgLjOz/SftdOZSxqkB8WY2KbvXwABvyqw
20, 37 -- References: {200907141243.n6EChd4E012051-at-ns.microscopy.com}
20, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
20, 37 -- To: {jehrman-at-mta.ca}
20, 37 -- Cc: {Microscopy-at-microscopy.com}
20, 37 -- X-OriginalArrivalTime: 14 Jul 2009 14:34:56.0300 (UTC) FILETIME=[42066EC0:01CA0490]
20, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.7.14.142115
20, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_AGE_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
20, 37 -- Content-Transfer-Encoding: 8bit
20, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6EEYv9g014526
==============================End of - Headers==============================




From: jtwilley-at-sprynet.com
Date: Tue, 14 Jul 2009 09:53:08 -0500
Subject: [Microscopy] Re: Mystery course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,

You are approximately correct about the late 70's date. I was in charge of failure analysis at Teledyne Microelectronics in the early '80s, located a short distance from the Beethoven St. address. Prior to that the company had relied upon SEAL for some forms of testing and I believe that one of the principals had formerly worked for Teledyne. As I recall, SEAL was at that address when I arrived at Teledyne in 1979 and then moved to another location roughly around 1982.

As to the notes, they might derive from an ISTFA course (International Symposium for Testing & Failure Analysis).

John Twilley

-----Original Message-----
} From: Frank_Karl-at-lincolnelectric.com
} Sent: Jul 14, 2009 8:43 AM
} To: jtwilley-at-sprynet.com
} Subject: [Microscopy] Mystery course
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
6, 25 -- From jtwilley-at-sprynet.com Tue Jul 14 09:53:08 2009
6, 25 -- Received: from elasmtp-scoter.atl.sa.earthlink.net (elasmtp-scoter.atl.sa.earthlink.net [209.86.89.67])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EEr7sk029569
6, 25 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 09:53:08 -0500
6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 25 -- s=dk20050327; d=sprynet.com;
6, 25 -- b=UxxBqJZqMkexbiZENhbgdAxTH4yUDdxYyTLPhpqBK1keZkZqZkCwrPY6ufeBqcep;
6, 25 -- h=Message-ID:Date:From:Reply-To:To:Subject:Mime-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-ELNK-Trace:X-Originating-IP;
6, 25 -- Received: from [209.86.224.31] (helo=elwamui-chisos.atl.sa.earthlink.net)
6, 25 -- by elasmtp-scoter.atl.sa.earthlink.net with esmtpa (Exim 4.67)
6, 25 -- (envelope-from {jtwilley-at-sprynet.com} )
6, 25 -- id 1MQjNT-0006HJ-Mh; Tue, 14 Jul 2009 10:53:07 -0400
6, 25 -- Received: from 70.23.75.247 by webmail.earthlink.net with HTTP; Tue, 14 Jul 2009 10:53:07 -0400
6, 25 -- Message-ID: {33260823.1247583187648.JavaMail.root-at-elwamui-chisos.atl.sa.earthlink.net}
6, 25 -- Date: Tue, 14 Jul 2009 09:53:07 -0500 (GMT-05:00)
6, 25 -- From: jtwilley-at-sprynet.com
6, 25 -- Reply-To: jtwilley-at-sprynet.com
6, 25 -- To: Frank_Karl-at-lincolnelectric.com, microscopy-at-microscopy.com
6, 25 -- Subject: Re: [Microscopy] Mystery course
6, 25 -- Mime-Version: 1.0
6, 25 -- Content-Type: text/plain; charset=UTF-8
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- X-Mailer: EarthLink Zoo Mail 1.0
6, 25 -- X-ELNK-Trace: adbb89a220ca650b5741bb2dafe8270584f945684cbf6968be0f0012ffba3f52db1c5d33308c2610350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
6, 25 -- X-Originating-IP: 209.86.224.31
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Tue, 14 Jul 2009 11:11:38 -0500
Subject: [Microscopy] Re: Oxford Inca (ISIS) system error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks Warren,

I'd very much appreciate any info you (or other listers) have on
potential replacement power supplies. I'll happily pay shipping, etc.
for any parts sent to me. Beyond that I'd have to check with
Administration, but it's not inconceivable that we could pay a bit to
take a dusty box off someone's hands.

X-from Warren's info it sounds like an ISIS system power supply might be
compatible with the Inca? Our system actually came with both software
suites, so I'm guessing the hardware is pretty much all the same...

Thanking all in advance,

Jim

Straszheim, Warren E [M S E] wrote:
} Good morning, Jim.
}
} We have a venerable ISIS system here that we are using. Personally, I
} like the ISIS interface better than the INCA interface. One of these
} days I will have to do something about it, but not just yet.
}
} A couple years back, we encountered similar errors indicating that one
} or more of our voltages were out of spec. There are test points behind
} the front panel that you could check with a voltmeter. There is also a
} validation utility program as part of the ISIS package that should let
} you check the voltages at will. I would have to check my notes for the
} details on using it.
}
} Anyway, we confirmed that our power supply was failing. Oxford could
} supply a replacement, but it would cost quite a bit. Instead, I put the
} word out on this list figuring there must be some ISIS units taken out
} of service and gathering dust somewhere or another. Indeed there were. I
} got two replies about two such systems and secured one and had it
} shipped to me. (Thanks, Scott.) It was a fairly simple matter to swap
} out the power supply module and we are still running with that module.
}
} I don't know if the second unit is still out there, but I could dig up
} the info. Probably there are other units that have been retired since I
} checked. This community is gracious enough that you could probably
} secure one without too much trouble.
}
} Warren
}
} -----Original Message-----
} From: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca]
} Sent: Tuesday, July 14, 2009 7:44 AM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Oxford Inca (ISIS) system error
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} Good morning listers,
}
} Lately I've been getting the following error message on startup of our
} Oxford Inca 200 system:
}
} Isis System Error
} Isis Link Driver:
} fault (Isis hardware)
} Error 85,[0] 15.54V Analog 15V above limit {00,00}
}
} So far this message has only appeared on startup and when the system is
} idle - doesn't seem to crop up when I'm actually working with the
} system, and seems to show up less frequently with time after the
} software is started. Also so far the "measured" over voltage is always
} exactly 15.54V, so my thinking is that it is some digital value that is
} just one bit off acceptable. I can dismiss the error and everything
} seems to be working normally.
}
} Any ideas out there? Livable nuisance? Easy no-brainer fix? Omen of
} impending doom? The system is coming up on 10 years old, but we really
} don't have the use to justify (or the desire) scrounging for funds to
} upgrade the system, so anything I can do to keep it breathing would be
} worthwhile.
}
} As always, thanks in advance,
}
} Jim
}
}
} ------------------------------------------------------------------------
}
}
} No virus found in this incoming message.
} Checked by AVG - www.avg.com
} Version: 8.5.387 / Virus Database: 270.13.13/2237 - Release Date: 07/14/09 05:56:00
}
}


--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
11, 21 -- From jehrman-at-mta.ca Tue Jul 14 11:11:38 2009
11, 21 -- Received: from mailgate2.mta.ca (mailgate2.mta.ca [138.73.1.200])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EGBbYU016346
11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 11:11:37 -0500
11, 21 -- Received: from host-22-194.mta.ca ([138.73.22.194]:51776)
11, 21 -- by mailgate2.mta.ca (smtp.mta.ca [138.73.1.130]:25)
11, 21 -- with esmtp id 1MQkeq-0005nt-Fu (Exim 4.69)
11, 21 -- (return-path {jehrman-at-mta.ca} ); Tue, 14 Jul 2009 13:15:08 -0300
11, 21 -- Message-ID: {4A5CACD0.1050402-at-mta.ca}
11, 21 -- Date: Tue, 14 Jul 2009 13:05:36 -0300
11, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
11, 21 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
11, 21 -- MIME-Version: 1.0
11, 21 -- To: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
11, 21 -- CC: Microscopy-at-microscopy.com
11, 21 -- Subject: Re: [Microscopy] Oxford Inca (ISIS) system error
11, 21 -- References: {200907141243.n6EChd4E012051-at-ns.microscopy.com} {16A330AC32056A40B32842EC4BB8D72703FFF362-at-maire.eng.iastate.edu}
11, 21 -- In-Reply-To: {16A330AC32056A40B32842EC4BB8D72703FFF362-at-maire.eng.iastate.edu}
11, 21 -- X-Enigmail-Version: 0.95.7
11, 21 -- Content-Type: text/plain; charset=ISO-8859-1
11, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: bthomson-at-21cm.com
Date: Tue, 14 Jul 2009 12:13:52 -0500
Subject: [Microscopy] Re: Mystery Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,
I used to work at SEAL Labs where it is now located in El Segundo, CA on
250 North Nash street - before I switched back to Bio.
It is a good and reputable failure analysis laboratory where they also
do destructive physical analysis as well as various analyses for
customers.
They used to give seminars way back when, and supposedly they still do,
but I never saw that happen while I was working there.
Arun Kumar, president of the company, is still there with other good
knowledgeable people.


Bruce Thomson
Microscopist, Imaging
ph. (909) 466-8633
fax (909) 466-8618
bthomson-at-21cm.com

21st Century Medicine
14960 Hilton Drive
Fontana, CA. 92336
www.21cm.com




This message has been scanned for any potential malware.


==============================Original Headers==============================
9, 32 -- From bthomson-at-21cm.com Tue Jul 14 12:13:52 2009
9, 32 -- Received: from p02c12o142.mxlogic.net (p02c12o142.mxlogic.net [208.65.145.75])
9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6EHDjT5001659
9, 32 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jul 2009 12:13:51 -0500
9, 32 -- Received: from unknown [74.212.219.34] (EHLO 21cm.com)
9, 32 -- by p02c12o142.mxlogic.net (mxl_mta-6.2.0-4)
9, 32 -- with ESMTP id 7ccbc5a4.2608057232.500260.00-019.p02c12o142.mxlogic.net (envelope-from {bthomson-at-21cm.com} );
9, 32 -- Tue, 14 Jul 2009 11:13:51 -0600 (MDT)
9, 32 -- Content-class: urn:content-classes:message
9, 32 -- MIME-Version: 1.0
9, 32 -- Content-Type: text/plain;
9, 32 -- charset="us-ascii"
9, 32 -- Subject: Re: Mystery Course
9, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
9, 32 -- Date: Tue, 14 Jul 2009 10:13:42 -0700
9, 32 -- Message-ID: {824E5F5FF9A5E74480853FD0B40C4BBC0C645B-at-Server1.21CM.local}
9, 32 -- X-MS-Has-Attach:
9, 32 -- X-MS-TNEF-Correlator:
9, 32 -- Thread-Topic: Re: Mystery Course
9, 32 -- Thread-Index: AcoEpm/+MQmfUm5oS5idsHgbiLJgpg==
9, 32 -- From: "Bruce Thomson" {bthomson-at-21cm.com}
9, 32 -- To: {microscopy-at-microscopy.com}
9, 32 -- X-Processed-By: Rebuild v2.0-0
9, 32 -- X-Spam: [F=0.2000000000; CM=0.500; S=0.200(2009070901)]
9, 32 -- X-MAIL-FROM: {bthomson-at-21cm.com}
9, 32 -- X-SOURCE-IP: [74.212.219.34]
9, 32 -- X-AnalysisOut: [v=1.0 c=1 a=rpGmfqynNp5hD1BdSTXp5A==:17 a=c38n3t_TAAAA:8 a]
9, 32 -- X-AnalysisOut: [=4kkeDm8qJHyK6pAyN94A:9 a=ftnBR44Iddet1mqu5iY6KAsZ-xMA:4 a]
9, 32 -- X-AnalysisOut: [=WJ_dtGxla2IA:10 a=M3OdVr6-8VEA:10 a=QEXaimFis7IA:10 a=i_Q]
9, 32 -- X-AnalysisOut: [9QRP--P4A:10 a=fD4zNOj7GI4A:10 a=IXW8o0AqNScA:10]
9, 32 -- Content-Transfer-Encoding: 8bit
9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6EHDjT5001659
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Wed, 15 Jul 2009 07:46:55 -0500
Subject: [Microscopy] viaWWW: TEM: Image distortion on omega filtered JEOL 2200FS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: David Mitchell

Organization: EM Unit, University of Sydney

Title-Subject: [Filtered] TEM: Image distortion on omega filtered JEOL 2200FS

Question: Dear Listers

I have recently started using our new JEOL 2200FS. I have been doing
energy filtered diffraction and have noticed that there is elliptical
distortion in electron diffraction patterns from polycrystalline thin
films. I have optimised (as best I know how to), the isochromaticity,
the achromaticity and the intermediate stigmation. The best result I
can get is a distortion of 2-3%. There is no automated image
distortion correction with an Omega filter as there is with a GIF.
The GIF routinely manages distortions {0.8% with its correction.

Question: Has anyone measured image distortion for the 2200FS and if
so, could you please supply me with some numbers? I'd like to know
if I can do better. This magnitude of distortion will impact on my
planned RDF work.

Thanks and regards,

Dave Mitchell

Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au


Login Host: 129.78.64.100
---------------------------------------------------------------------------

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Wed Jul 15 07:46:55 2009
14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6FCksHK030361
14, 11 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jul 2009 07:46:54 -0500
14, 11 -- Mime-Version: 1.0
14, 11 -- Message-Id: {p06240800c683802d3e5e-at-[206.69.208.22]}
14, 11 -- Date: Wed, 15 Jul 2009 07:46:53 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
14, 11 -- Subject: viaWWW: TEM: Image distortion on omega filtered JEOL 2200FS
14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Thu, 16 Jul 2009 07:21:31 -0500
Subject: [Microscopy] Followup: Oxford Inca (ISIS) system error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning listers,

First, thanks as usual to all who have offered assistance and
suggestions. What a truly indispensable resource the Listserv is!

I'm tracking down solutions to my problem (most likely replacing the
power supply) but in the course of planning I've come up with some
additional questions:

1. Is it better to power down the system when not in use (ostensibly to
preserve the failing power supply) or is that going to add fuel to the
fire and further weaken compromised components?
2. Come to think of it, even with a healthy system, is it better to
power on only when the system is being used, or leave it on 24/7? We use
EDS something less than once a week.
3. When I disconnect the Inca box to fiddle with it, and possibly send
the power supply off for repair, is it better to keep the detector cool
or do a warmup?
4. Does anyone know for certain whether the power supplies for the Isis
systems are the same as the blue-box Inca systems?

Thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
10, 18 -- From jehrman-at-mta.ca Thu Jul 16 07:21:30 2009
10, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.202])
10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6GCLTfJ015094
10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jul 2009 07:21:30 -0500
10, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:49986)
10, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
10, 18 -- with esmtp id 1MRPy3-0001Kk-SN (Exim 4.69) for Microscopy-at-microscopy.com
10, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 16 Jul 2009 09:21:43 -0300
10, 18 -- Message-ID: {4A5F19E1.8010206-at-mta.ca}
10, 18 -- Date: Thu, 16 Jul 2009 09:15:29 -0300
10, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
10, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
10, 18 -- MIME-Version: 1.0
10, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
10, 18 -- Subject: Followup: Oxford Inca (ISIS) system error
10, 18 -- X-Enigmail-Version: 0.95.7
10, 18 -- Content-Type: text/plain; charset=ISO-8859-1
10, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 16 Jul 2009 08:37:30 -0500
Subject: [Microscopy] LaB6 Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to emit a big thank you for all the responses to my inquiry
about LaB6 sources. The winners seem to be Kimball and Denka, in that
order.

May your futures be stable and bright and founded upon an indestructible
base.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original Headers==============================
6, 27 -- From TindallR-at-missouri.edu Thu Jul 16 08:37:29 2009
6, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6GDbSjc001037
6, 27 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jul 2009 08:37:29 -0500
6, 27 -- X-IronPort-Anti-Spam-Filtered: true
6, 27 -- X-IronPort-Anti-Spam-Result: ApoEAGXKXkrRauUo/2dsb2JhbADBCwEJiASIT4JOgT0FgUA
6, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
6, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Jul 2009 08:37:13 -0500
6, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Thu, 16 Jul 2009 08:37:13 -0500
6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 27 -- Content-class: urn:content-classes:message
6, 27 -- MIME-Version: 1.0
6, 27 -- Content-Type: text/plain;
6, 27 -- charset="us-ascii"
6, 27 -- Subject: LaB6 Emitters
6, 27 -- Date: Thu, 16 Jul 2009 08:37:13 -0500
6, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8242-at-UM-XMAIL08.um.umsystem.edu}
6, 27 -- X-MS-Has-Attach:
6, 27 -- X-MS-TNEF-Correlator:
6, 27 -- Thread-Topic: LaB6 Emitters
6, 27 -- Thread-Index: AcoGGotQx7HScjmRT2eYweMCa6gPGg==
6, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
6, 27 -- To: {microscopy-at-microscopy.com}
6, 27 -- X-OriginalArrivalTime: 16 Jul 2009 13:37:13.0864 (UTC) FILETIME=[87142080:01CA061A]
6, 27 -- Content-Transfer-Encoding: 8bit
6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6GDbSjc001037
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 17 Jul 2009 13:04:37 -0500
Subject: [Microscopy] viaWWW: out of focus raster on S4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Derrick,
X-from your description, my first inclination is to say that the problem is
your sample. If changing apertures changes the focus problem, it would look
as though the sample was charging and discharging. Some samples can do this
in a very periodic fashion at certain beam currents and scan rates.
Changing apertures, condenser lens settings, scan rates, accelerating
voltage, etc. will change the response.

Another possibility is that the stage ground is intermittent, so sometimes
the entire stage charges, then discharges, while at other times everything
behaves fine. Sometimes this is a problem in the rotation function, as it
requires a sliding contact rather than a wire held by a screw.

If your stage ground is good and the problem sometimes occurs with known
conductive samples, then there is a possibility that the high voltage is not
stable. There are many possibilities here. I'm assuming that you are using
digital capture so that the electronics involved in a recording CRT are not
the issue.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: dhorne-at-interchange.ubc.ca [mailto:dhorne-at-interchange.ubc.ca]
Sent: Monday, July 13, 2009 7:03 PM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dhorne-at-interchange.ubc.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] out of focus raster on S4700

Question: Our S4700 demonstrated a new problem the other day.

During an image acquisition in line capture mode, there were
alternating bands that were in and out of focus along the x axis.
This could not be explained by anything to do with the sample (i.e.
regular, repeating, large-magnitude topographical changes), and was
occurring at all mags that we checked. I believe they were there
with varying degrees of raster rotation engaged as well. I did not
measure the spacing, but it did look as if the distortion was
repeating at regular intervals.

After realigning the scope, noting there was some charging at
spacings in the cuticle at the edges of the image, and changing to a
different objective aperture, I was able to minimize but not
eliminate the problem. It did not reappear at startup this week.

Can anyone offer an explanation to how this distortion could be
caused? I'm particularly interested in the electronics side of it, as
this has been our bugbear over the last few years.

Thanks in advance

Derrick

Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Mon Jul 13 17:57:22 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n6DMvL9Q002566
11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jul 2009 17:57:22
-0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240800c6816c3c8f4d-at-[206.69.208.22]}
11, 11 -- Date: Mon, 13 Jul 2009 17:57:21 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: out of focus raster on S4700
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Fri Jul 17 13:04:37 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6HI4bJE021523
25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2009 13:04:37 -0500
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20090717180436789.ZJPN28563-at-cdptpa-omta02.mail.rr.com} ;
25, 25 -- Fri, 17 Jul 2009 18:04:36 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: {dhorne-at-interchange.ubc.ca} , "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] viaWWW: out of focus raster on S4700
25, 25 -- Date: Fri, 17 Jul 2009 14:04:28 -0400
25, 25 -- Message-ID: {02364577D41643A8B4B12ED727080CCE-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="US-ASCII"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- In-Reply-To: {200907132303.n6DN3Suk006609-at-ns.microscopy.com}
25, 25 -- Importance: Normal
25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- thread-index: AcoEDiWUJyFq8EVAQ5C4W7BBRTsb+gC+E1iw
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6HI4bJE021523
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 17 Jul 2009 17:09:30 -0500
Subject: [Microscopy] Making Posters for Meetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm planning an article for Microscopy Today on the production of
large format posters for scientific meetings.

Although the article will deal primarily with Adobe InDesign, I'm very
interested in finding out what programs others use to produce posters.

Also, if you would share any hints or protocols used to produce your
posters (including esthetic considerations), I will attempt to
integrate them into the discussion.

Thanks, everyone.


John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901

==============================Original Headers==============================
6, 16 -- From bozzola-at-siu.edu Fri Jul 17 17:09:30 2009
6, 16 -- Received: from mail-qy0-f199.google.com (mail-qy0-f199.google.com [209.85.221.199])
6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6HM9UBQ019634
6, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jul 2009 17:09:30 -0500
6, 16 -- Received: by qyk37 with SMTP id 37so921790qyk.10
6, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jul 2009 15:09:30 -0700 (PDT)
6, 16 -- MIME-Version: 1.0
6, 16 -- Received: by 10.224.6.148 with SMTP id 20mr1120263qaz.203.1247868569471; Fri,
6, 16 -- 17 Jul 2009 15:09:29 -0700 (PDT)
6, 16 -- Date: Fri, 17 Jul 2009 17:09:29 -0500
6, 16 -- Message-ID: {ebc2299e0907171509j1a647f4ax4355d01d09f4e54-at-mail.gmail.com}
6, 16 -- Subject: Making Posters for Meetings
6, 16 -- From: John Bozzola {bozzola-at-siu.edu}
6, 16 -- To: MSAListserver {Microscopy-at-microscopy.com}
6, 16 -- Content-Type: text/plain; charset=UTF-8
6, 16 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Mon, 20 Jul 2009 14:58:33 -0500
Subject: [Microscopy] Preserving plaque for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I seem to remember that my friends at Link Systems, now Oxford, would run
the systems in their demo labs all of the time. I also remember when
working with the Edax team they would run a system for three weeks prior to
using it for demonstrations; its all about stability!

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com

----- Original Message -----
X-from: {jehrman-at-mta.ca}
To: {protrain-at-emcourses.com}
Sent: Thursday, July 16, 2009 1:24 PM

Hi, All-

I am looking at diamond-studded burrs used for drilling through
atherosclerotic plaque in cardiac patients. We would like to preserve the
material that sticks to the burr for SEM. Being a biologist, if I were
looking at heart tissue, I'd know what to do. But this is more a mix of
goop and materials science... The burr is made of steel, cobalt, diamonds,
and I'm not sure what else. It corroded like mad in bleach. Not sure what
it would do in a critical point dryer.

Any suggestions? Shall I send fixative into the operating room, which I
hesitate to do? Or something else?

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
6, 19 -- From tina-at-pbrc.hawaii.edu Mon Jul 20 14:58:33 2009
6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6KJwWm2021718
6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Jul 2009 14:58:33 -0500
6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n6KJwThv005269
6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Jul 2009 09:58:30 -1000 (HST)
6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n6KJwSBM005266
6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Jul 2009 09:58:28 -1000 (HST)
6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
6, 19 -- Date: Mon, 20 Jul 2009 09:58:28 -1000 (HST)
6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
6, 19 -- X-Sender: tina-at-halia
6, 19 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 19 -- Subject: Preserving plaque for SEM
6, 19 -- Message-ID: {Pine.GSO.4.21.0907200943560.4964-100000-at-halia}
6, 19 -- MIME-Version: 1.0
6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: nyilmaz-at-mersin.edu.tr
Date: Tue, 21 Jul 2009 02:31:38 -0500
Subject: [Microscopy] donation request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleaques,
We are expecting a quite busy period in terms of research studies.
As some of you do also experiencing similiar issues,we are using fluorescent
microscopes from other depts when available.
But mostly it is not easy. Buying a new one does not seem possible soon due
to financial
issues. My question is, if any colleague has a redundant fluorescein
microscope available to donate us. Your kindly helps would be greatly
appreciated.
Thanks in advance.

Dr. Necat Yilmaz MD, PhD
University of Mersin
School of Medicine
Histology and Embryology Dept.
Mersin/TURKEY


==============================Original Headers==============================
3, 31 -- From nyilmaz-at-mersin.edu.tr Tue Jul 21 02:31:37 2009
3, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6L7Vahb004646
3, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 02:31:37 -0500
3, 31 -- Received: from localhost (localhost [127.0.0.1])
3, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 45C512F9BB3
3, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 10:31:02 +0300 (EEST)
3, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr
3, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1])
3, 31 -- by localhost (mail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024)
3, 31 -- with ESMTP id lmsK+zYJE+Tj for {Microscopy-at-microscopy.com} ;
3, 31 -- Tue, 21 Jul 2009 10:30:57 +0300 (EEST)
3, 31 -- Received: from nejat1 (unknown [193.255.129.131])
3, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 459DF2F9B82
3, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 10:30:57 +0300 (EEST)
3, 31 -- Message-ID: {000201ca09d5$3eb12040$2101a8c0-at-nejat1}
3, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
3, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com}
3, 31 -- Subject: donation request
3, 31 -- Date: Mon, 20 Jul 2009 23:15:40 +0300
3, 31 -- MIME-Version: 1.0
3, 31 -- Content-Type: text/plain;
3, 31 -- format=flowed;
3, 31 -- charset="iso-8859-9";
3, 31 -- reply-type=original
3, 31 -- Content-Transfer-Encoding: 7bit
3, 31 -- X-Priority: 3
3, 31 -- X-MSMail-Priority: Normal
3, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138
3, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
3, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Tue, 21 Jul 2009 10:12:04 -0500
Subject: [Microscopy] Meeting Announcement: Midwest Microscopy and Microanalysis Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All,

The next meeting of the Midwest Microscopy and Microanalysis Society
will be held on Wednesday, August 26, 2009, at the University of
Illinois at Chicago. The program is entitled, "Materials Electron
Microscopy at the Limit", and Robert Klie has organized an exciting
lineup of speakers to discuss advanced electron microscopy in materials
science. Details of the meeting can be found on our website under
Meetings:

www.midwestmicroscopy.org


We look forward to seeing you then, if not sooner in Richmond!

All the best,

Elaine Schumacher
M3S Program Coordinator


*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com}
Web Site: www.mccrone.com {http://www.mccrone.com/}







==============================Original Headers==============================
13, 23 -- From eschumacher-at-mccrone.com Tue Jul 21 10:12:04 2009
13, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6LFC2hX012492
13, 23 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 10:12:03 -0500
13, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959);
13, 23 -- Tue, 21 Jul 2009 10:11:19 -0500
13, 23 -- Content-class: urn:content-classes:message
13, 23 -- MIME-Version: 1.0
13, 23 -- Content-Type: text/plain;
13, 23 -- charset="us-ascii"
13, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society
13, 23 -- Date: Tue, 21 Jul 2009 10:11:19 -0500
13, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A702307E82-at-MCCRONEMSG.tmg.mccrone.com}
13, 23 -- X-MS-Has-Attach:
13, 23 -- X-MS-TNEF-Correlator:
13, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society
13, 23 -- Thread-Index: AcoKFX/0mRF/WuqWRrOlepIaJFIQ9A==
13, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
13, 23 -- To: {microscopy-at-microscopy.com}
13, 23 -- X-OriginalArrivalTime: 21 Jul 2009 15:11:19.0492 (UTC) FILETIME=[80335840:01CA0A15]
13, 23 -- Content-Transfer-Encoding: 8bit
13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6LFC2hX012492
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Tue, 21 Jul 2009 14:41:41 -0500
Subject: [Microscopy] Hot Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm wondering if anyone out there might have a SEM with the following
ability and the willingness to assist us?

1) A Hot Stage able to go to 450deg C.

2) Ability to create a movie file(avi, mpg, etc..) while imaging with the SEM.

3) Time to run 4 samples through 2 different heating ramp profiles.
We think the total time involved would be about 15mins per sample.

The samples are pieces of silicon wafers and contain features which
we'd like to image while heating to better understand the changes that
take place when these features are heated. The feature we'd be
looking at would be between 10-30um in diameter with a copper top
layer.

Please let me know offline if you might be interested in helping.

Thanks in advance!

==============================Original Headers==============================
7, 31 -- From ian.drucker-at-gmail.com Tue Jul 21 14:41:40 2009
7, 31 -- Received: from mail-yx0-f190.google.com (mail-yx0-f190.google.com [209.85.210.190])
7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6LJfdDb009121
7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 14:41:40 -0500
7, 31 -- Received: by yxe28 with SMTP id 28so5835639yxe.10
7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2009 12:41:38 -0700 (PDT)
7, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 31 -- d=gmail.com; s=gamma;
7, 31 -- h=domainkey-signature:mime-version:received:from:date:message-id
7, 31 -- :subject:to:content-type:content-transfer-encoding;
7, 31 -- bh=Vg32S5Rsl/dzN1eKxS4yEug3eG4RFPFwJsghDit0y5o=;
7, 31 -- b=scq4uRHuTgXDWWB+WXkJdDiukqofabsn9/4vk5FEpMGpt5Q+X7cnE6mZTwF9u0AwWi
7, 31 -- 7JVA+wSQZONtv7qMxhQHSt+VtxjN0w/aDP/SKCH1bqTmgl8lMxyXGptybTCMtJKE7Aa1
7, 31 -- CNEG+BD8bpyBdjHxEXHg+MjT229AuxNYacHVU=
7, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 31 -- d=gmail.com; s=gamma;
7, 31 -- h=mime-version:from:date:message-id:subject:to:content-type
7, 31 -- :content-transfer-encoding;
7, 31 -- b=cf7IYHGTHpWi0OXofOOeoD+FCJ3xIyD8nzYYtXWB1m3HaulTxCpPJNUxrPlhRYkHYu
7, 31 -- fJaRI7LDVyPiZ+zdsLMTfhziR2lWrFDRy7YCjbna8u/SfD9Rra2j4pYE2VELWh16sawI
7, 31 -- 8MoF2bmcDdik70kK68HkpuE/cH1MlveJzHe7k=
7, 31 -- MIME-Version: 1.0
7, 31 -- Received: by 10.100.166.13 with SMTP id o13mr53625ane.103.1248205298237; Tue,
7, 31 -- 21 Jul 2009 12:41:38 -0700 (PDT)
7, 31 -- From: ID {ian.drucker-at-gmail.com}
7, 31 -- Date: Tue, 21 Jul 2009 13:41:18 -0600
7, 31 -- Message-ID: {c0bfda950907211241q434356abx499e95690063d053-at-mail.gmail.com}
7, 31 -- Subject: Hot Stage
7, 31 -- To: Microscopy-at-microscopy.com
7, 31 -- Content-Type: text/plain; charset=ISO-8859-1
7, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nasi-at-imem.cnr.it
Date: Wed, 22 Jul 2009 09:18:12 -0500
Subject: [Microscopy] viaWWW: TEM cross section preparation of CIGS/glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nasi-at-imem.cnr.it as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nasi-at-imem.cnr.it
Name: Lucia Nasi

Organization: CNR-IMEM Institute, Parma Italy

Title-Subject: [Filtered] TEM cross section preparation of CIGS/glass

Question: Hi all,
does anyone have experience with the preparation of
CIGS/glass for TEM cross section analysis?

Thank you,
Lucia.

--
Lucia Nasi

CNR-IMEM Institute
V.le Usberti 37/A
43100 Parma (Italy)
www.imem.cnr.it

e-mail nasi-at-imem.cnr.it
tel +39 0521 269285
fax +39 0521 269206

Login Host: 192.167.161.20
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Jul 22 09:18:12 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6MEIAOw026560
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jul 2009 09:18:11 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c68cd010537c-at-[206.69.208.22]}
10, 11 -- Date: Wed, 22 Jul 2009 09:18:09 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: nasi-at-imem.cnr.it (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TEM cross section preparation of CIGS/glass
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nasi-at-imem.cnr.it
Date: Wed, 22 Jul 2009 09:44:53 -0500
Subject: [Microscopy] CIGS/glass preparation for TEM cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all!
Does anyone have experience with the preparation of CIGS/glass for TEM
cross section analysis?
Thank you,
Lucia.

--
Lucia Nasi

CNR-IMEM Institute
V.le Usberti 37/A
43100 Parma (Italy)
www.imem.cnr.it

e-mail nasi-at-imem.cnr.it
tel +39 0521 269285
fax +39 0521 269206



==============================Original Headers==============================
7, 25 -- From nasi-at-imem.cnr.it Wed Jul 22 09:44:53 2009
7, 25 -- Received: from area.bo.cnr.it (area.bo.cnr.it [192.167.160.13])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6MEiq57010183
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2009 09:44:53 -0500
7, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 25 -- by area.bo.cnr.it (Postfix) with ESMTP id E24DD4B0079
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2009 16:44:51 +0200 (CEST)
7, 25 -- X-Virus-Scanned: amavisd-new at area.bo.cnr.it
7, 25 -- Received: from area.bo.cnr.it ([127.0.0.1])
7, 25 -- by localhost (area.bo.cnr.it [127.0.0.1]) (amavisd-new, port 10024)
7, 25 -- with ESMTP id iarE02Ikhj0D for {Microscopy-at-microscopy.com} ;
7, 25 -- Wed, 22 Jul 2009 16:44:35 +0200 (CEST)
7, 25 -- Received: from [127.0.0.1] (lucia.imem.cnr.it [160.78.60.58])
7, 25 -- by area.bo.cnr.it (Postfix) with ESMTP id 3EABE4B0078
7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2009 16:44:35 +0200 (CEST)
7, 25 -- Message-ID: {4A6725CE.3020000-at-imem.cnr.it}
7, 25 -- Date: Wed, 22 Jul 2009 16:44:30 +0200
7, 25 -- From: Lucia Nasi {nasi-at-imem.cnr.it}
7, 25 -- Organization: CNR-IMEM
7, 25 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
7, 25 -- MIME-Version: 1.0
7, 25 -- To: Microscopy-at-microscopy.com
7, 25 -- Subject: CIGS/glass preparation for TEM cross sections
7, 25 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 25 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jerzy.gazda-at-ceriumlabs.com
Date: Wed, 22 Jul 2009 14:48:55 -0500
Subject: [Microscopy] viaWWW: TEM cross section preparation of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lucia,
What in particular do you need to know? We have some experience working
with FIB tools to prepare TEM cross-sections of CIGS solar cells on
glass substrates.

Jerzy
www.CeriumLabs.com
-----Original Message-----
X-from: nasi-at-imem.cnr.it [mailto:nasi-at-imem.cnr.it]
Sent: Wednesday, July 22, 2009 9:41 AM
To: Gazda, Jerzy

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both nasi-at-imem.cnr.it as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: nasi-at-imem.cnr.it
Name: Lucia Nasi

Organization: CNR-IMEM Institute, Parma Italy

Title-Subject: [Filtered] TEM cross section preparation of CIGS/glass

Question: Hi all,
does anyone have experience with the preparation of
CIGS/glass for TEM cross section analysis?

Thank you,
Lucia.

--
Lucia Nasi

CNR-IMEM Institute
V.le Usberti 37/A
43100 Parma (Italy)
www.imem.cnr.it

e-mail nasi-at-imem.cnr.it
tel +39 0521 269285
fax +39 0521 269206

Login Host: 192.167.161.20
------------------------------------------------------------------------
---

==============================Original
Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Jul 22 09:18:12 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n6MEIAOw026560
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jul 2009
09:18:11 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c68cd010537c-at-[206.69.208.22]}
10, 11 -- Date: Wed, 22 Jul 2009 09:18:09 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: nasi-at-imem.cnr.it (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TEM cross section preparation of CIGS/glass
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
17, 29 -- From Jerzy.Gazda-at-spansion.com Wed Jul 22 14:48:55 2009
17, 29 -- Received: from usausmgw02.spansion.com (usausmgw02.spansion.com [12.110.209.162])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6MJmtL8013778
17, 29 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jul 2009 14:48:55 -0500
17, 29 -- X-IronPort-AV: E=McAfee;i="5300,2777,5684"; a="4347927"
17, 29 -- Received: from usausexbh2.spansion.com ([10.248.26.116])
17, 29 -- by usausmgw02.spansion.com with ESMTP; 22 Jul 2009 12:48:54 -0700
17, 29 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by usausexbh2.spansion.com with Microsoft SMTPSVC(6.0.3790.3959);
17, 29 -- Wed, 22 Jul 2009 14:48:54 -0500
17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 29 -- Content-class: urn:content-classes:message
17, 29 -- MIME-Version: 1.0
17, 29 -- Content-Type: text/plain;
17, 29 -- charset="us-ascii"
17, 29 -- Subject: RE: [Microscopy] viaWWW: TEM cross section preparation of CIGS/glass
17, 29 -- Date: Wed, 22 Jul 2009 14:48:54 -0500
17, 29 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158FCA79A-at-USAUSEXMBPF1.spansion.com}
17, 29 -- In-Reply-To: {200907221441.n6MEfGkg009680-at-ns.microscopy.com}
17, 29 -- X-MS-Has-Attach:
17, 29 -- X-MS-TNEF-Correlator:
17, 29 -- Thread-Topic: [Microscopy] viaWWW: TEM cross section preparation of CIGS/glass
17, 29 -- Thread-Index: AcoK2nyRE03yAK6+STaK0BFs947ZywAKqxJg
17, 29 -- References: {200907221441.n6MEfGkg009680-at-ns.microscopy.com}
17, 29 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com}
17, 29 -- To: {nasi-at-imem.cnr.it}
17, 29 -- Cc: {microscopy-at-microscopy.com}
17, 29 -- X-OriginalArrivalTime: 22 Jul 2009 19:48:54.0963 (UTC) FILETIME=[720C4830:01CA0B05]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6MJmtL8013778
==============================End of - Headers==============================




From: Jared-at-lv-em.com
Date: Thu, 23 Jul 2009 08:52:01 -0500
Subject: [Microscopy] TEM - Sample Preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I was wondering if anyone would be able to recommend a comprehensive
text for TEM sample preparation. This text could be geared towards the
novice user or advanced.

Any input would be appreciated.

Thanks,


----------------------------------

Jared Lapkovsky

LVEM5 Team
Delong America Inc.

Montreal, Canada

514.904.1202
www.lv-em.com



==============================Original Headers==============================
12, 17 -- From Jared-at-lv-em.com Thu Jul 23 08:52:01 2009
12, 17 -- Received: from relais.videotron.ca (relais.videotron.ca [24.201.245.36])
12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6NDq0vY022463
12, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 23 Jul 2009 08:52:00 -0500
12, 17 -- MIME-version: 1.0
12, 17 -- Content-transfer-encoding: 7BIT
12, 17 -- Content-type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 17 -- Received: from [192.168.1.101] ([74.56.174.139]) by VL-MO-MR003.ip.videotron.ca
12, 17 -- (Sun Java(tm) System Messaging Server 6.3-4.01 (built Aug 3 2007; 32bit))
12, 17 -- with ESMTP id {0KN800480MINKK90-at-VL-MO-MR003.ip.videotron.ca} for
12, 17 -- Microscopy-at-Microscopy.Com; Thu, 23 Jul 2009 09:51:59 -0400 (EDT)
12, 17 -- Message-id: {EB01E508-91E9-4BE3-B039-32DB6354D7A7-at-lv-em.com}
12, 17 -- From: Jared Lapkovsky {Jared-at-lv-em.com}
12, 17 -- To: Microscopy-at-Microscopy.Com
12, 17 -- Subject: TEM - Sample Preparation
12, 17 -- Date: Thu, 23 Jul 2009 09:51:59 -0400
12, 17 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 23 Jul 2009 20:52:40 -0500
Subject: [Microscopy] Loevyou

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://evenguide.com



==============================Original Headers==============================
3, 21 -- From smithsoniantqt0767-at-richardson-roofing.com Thu Jul 23 20:52:37 2009
3, 21 -- Received: from 189-135-223-170-dyn.prod-infinitum.com.mx (dsl-189-135-223-170-dyn.prod-infinitum.com.mx [189.135.223.170] (may be forged))
3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O1qY2C032498;
3, 21 -- Thu, 23 Jul 2009 20:52:36 -0500
3, 21 -- Received: from [168.106.15.1] (helo=bvdoneewrejw.epzhvpmbg.info)
3, 21 -- by 189-135-223-170-dyn.prod-infinitum.com.mx with esmtpa (Exim 4.69)
3, 21 -- (envelope-from )
3, 21 -- id 1MM5ON-7212jx-5K
3, 21 -- for zaluzec-at-microscopy.com; Thu, 23 Jul 2009 19:52:36 -0600
3, 21 -- From: zaluzec-at-microscopy.com
3, 21 -- To: {zaluzec-at-microscopy.com}
3, 21 -- Subject: Loevyou
3, 21 -- Date: Thu, 23 Jul 2009 19:52:36 -0600
3, 21 -- MIME-Version: 1.0
3, 21 -- Content-Type: text/plain;
3, 21 -- charset="Windows-1252"
3, 21 -- Content-Transfer-Encoding: 7bit
3, 21 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353
3, 21 -- Thread-Index: Aca6QKWPTCTNGSB6XQPTZP20CZ3QOL==
3, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2741.2600
3, 21 -- Message-ID: {3147065930.2QAG18UO188340-at-tvilstrleybf.ubkzbclzqkgroz.org}
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 23 Jul 2009 20:58:56 -0500
Subject: [Microscopy] Administrivia: SPAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues....

A bit of spam just got through my filters. Bear with me while
I investigate and tighten up the filters even more.

My apologies.

Your Friendly Neighborhood SysOp
Nestor

==============================Original Headers==============================
4, 11 -- From zaluzec-at-microscopy.com Thu Jul 23 20:58:56 2009
4, 11 -- Received: from [172.16.0.112] (msdvpn072.msd.anl.gov [130.202.238.72])
4, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O1wtSo007899
4, 11 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jul 2009 20:58:56 -0500
4, 11 -- Mime-Version: 1.0
4, 11 -- Message-Id: {p06240801c68ec56b55d2-at-[206.69.208.26]}
4, 11 -- Date: Thu, 23 Jul 2009 20:58:54 -0500
4, 11 -- To: microscopy-at-microscopy.com
4, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
4, 11 -- Subject: Administrivia: SPAM
4, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hubner-at-m6.mech.pk.edu.pl
Date: Fri, 24 Jul 2009 03:45:13 -0500
Subject: [Microscopy] international conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends and colleagues,

I'll have to do something I've managed to avoid so far: section a cell
from monolayer defined by immunofluorescence under LM and... look for
the centrosome!
The first question I would like to ask, what do you like to use as a
substrate to grow the cells on for such case? I once bought some
coverslips with a kind of grid on them, years ago. Never used them.
They were individually wrapped and I thought were Nunc brand, but I
just looked on the Nunc website and could not find them, nor could I
find them in EM suppliers' catalogs. And no, I don't have them
anymore. Never used them and that was on my earlier job...
Can anyone tell me what they might have been, or perhaps suggest an
alternative? If I don't find a suitable marked substrate, then of
course taking plenty of pictures with the LM camera will do.

My second question is a humble request for any tips on how to best
embed so that I can then locate the cells in the block. I remember
someone's (sorry!..) tip fairly recently where just a thin layer of
epoxy was poured on top of the coverslip - is that what people do?
I remember my lab neighbors some 15 years ago would just put the
coverslip (Nevada-shaped) on the bottom of a round embedding well,
pour 3-4 mm resin on top, and that was it. They were then able to find
their cells in the block allright, although removing the glass was
occasionally a problem.

I do have experience embedding monolayers, just never had to section a
particular cell.

Thanks!
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

==============================Original Headers==============================
5, 22 -- From vladislav_speransky-at-nih.gov Thu Jul 23 22:16:47 2009
5, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O3GlaM030072
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jul 2009 22:16:47 -0500
5, 22 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
5, 22 -- by out1.messagingengine.com (Postfix) with ESMTP id EF7843BCAF2;
5, 22 -- Thu, 23 Jul 2009 23:16:46 -0400 (EDT)
5, 22 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
5, 22 -- by compute1.internal (MEProxy); Thu, 23 Jul 2009 23:16:46 -0400
5, 22 -- X-Sasl-enc: RGNsVkSeWrksq8lrY1GI1wNc5KWgIsm2C7HqXjJdwiLE 1248405406
5, 22 -- Received: from [192.168.1.5] (pool-173-66-201-136.washdc.fios.verizon.net [173.66.201.136])
5, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 9F2DE3202B;
5, 22 -- Thu, 23 Jul 2009 23:16:46 -0400 (EDT)
5, 22 -- Message-Id: {0E092A1B-3ADA-43EE-92BE-4405D0CAF97D-at-nih.gov}
5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
5, 22 -- To: Microscopy-at-microscopy.com
5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 22 -- Content-Transfer-Encoding: 7bit
5, 22 -- Subject: embedding monolayer to section a particular cell (2 questions)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
5, 22 -- Date: Thu, 23 Jul 2009 23:16:46 -0400
5, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================

From qc-at-crownofficechambers.com Fri Jul 24 02:40:29 2009
Return-Path: {qc-at-crownofficechambers.com}
Received: from mail.camden.net (mail.camden.net [67.222.164.3])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O7eTin014181;
Fri, 24 Jul 2009 02:40:29 -0500
Message-Id: {200907240740.n6O7eTin014181-at-ns.microscopy.com}
Received: from 123.172.7.131 [123.172.7.131] by mail.camden.net with SMTP;
Thu, 23 Jul 2009 20:57:21 -0400
Reply-To: {markturnerqc-at-hotmail.co.uk}


Hello,

Magnesium alloys and their applications - 8th international 26-29 oct 2009
Weimar Germany
link to www.dgm.de/magnesium

European symposium on superalloys an their appliactions 22 - 28 may 2010
Freiburg Germany,
link to www.dgm/superalloys

best regards

Krzysztof Hübner
Cracow University of Technology



==============================Original Headers==============================
8, 31 -- From hubner-at-m6.mech.pk.edu.pl Fri Jul 24 03:45:12 2009
8, 31 -- Received: from m6.mech.pk.edu.pl (m6.mech.pk.edu.pl [149.156.153.154])
8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O8jBpO017267
8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 03:45:12 -0500
8, 31 -- Received: from localhost (unknown [127.0.0.1])
8, 31 -- by m6.mech.pk.edu.pl (Postfix) with ESMTP id 705DF595
8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 11:12:45 +0200 (MEST)
8, 31 -- Received: from m6.mech.pk.edu.pl ([127.0.0.1])
8, 31 -- by localhost (m6 [127.0.0.1]) (amavisd-new, port 10024) with ESMTP
8, 31 -- id 09186-01 for {microscopy-at-microscopy.com} ;
8, 31 -- Fri, 24 Jul 2009 11:12:11 +0200 (MEST)
8, 31 -- Received: from Krzysiek (unknown [149.156.153.184])
8, 31 -- by m6.mech.pk.edu.pl (Postfix) with SMTP id 08BB03E3
8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 11:12:10 +0200 (MEST)
8, 31 -- Message-ID: {02B98D7953DF49BBA4DD747E2F285F1F-at-Krzysiek}
8, 31 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-m6.mech.pk.edu.pl}
8, 31 -- To: "Microscopy list" {microscopy-at-microscopy.com}
8, 31 -- Subject: international conference
8, 31 -- Date: Fri, 24 Jul 2009 10:44:33 +0200
8, 31 -- Organization: =?iso-8859-2?Q?Politechnika_Krakowska_Wydzia=B3_Mechaniczny?=
8, 31 -- MIME-Version: 1.0
8, 31 -- Content-Type: text/plain;
8, 31 -- format=flowed;
8, 31 -- charset="iso-8859-2";
8, 31 -- reply-type=original
8, 31 -- Content-Transfer-Encoding: 8bit
8, 31 -- X-Priority: 3
8, 31 -- X-MSMail-Priority: Normal
8, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
8, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
8, 31 -- X-Virus-Scanned: by amavisd-new at m6.mech.pk.edu.pl
==============================End of - Headers==============================




From: youngwk-at-snu.ac.kr
Date: Fri, 24 Jul 2009 04:58:37 -0500
Subject: [Microscopy] Looking for ASID scanning unit for JEOL TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
We're looking for scanning control unit for STEM operation in JEOL JEM-2010F
(or any X010 model) TEM.
If you have a unit not plan to use, please contact off list.
We will pay for the relocation and restoration cost with the purchase.
Thank you.

Young-Woon Kim
Professor
Department of Materials Science and Engineering
Seoul National University
Gwanak-gu Silim-dong San 56-1
Seoul, Republic of Korea ( Postal code 151-744)
Tel) +82-2-880-7977
Fax) +82-2-883-8197
E-mail) youngwk-at-snu.ac.kr







==============================Original Headers==============================
8, 28 -- From youngwk-at-snu.ac.kr Fri Jul 24 04:58:37 2009
8, 28 -- Received: from nabi3.snu.ac.kr (nabi3.snu.ac.kr [147.46.10.161])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6O9waud002074
8, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 04:58:37 -0500
8, 28 -- Received: from [147.46.10.145] ([147.46.10.145])
8, 28 -- by nabi3.snu.ac.kr ([147.46.10.161])
8, 28 -- with ESMTP id 2009072418:58:24:480093.4993.72956832
8, 28 -- for {Microscopy-at-microscopy.com} ;
8, 28 -- Fri, 24 Jul 2009 18:58:24 +0900 (KST)
8, 28 -- Received: from [147.46.233.110] ([147.46.233.110])
8, 28 -- by auk1.snu.ac.kr ([147.46.10.145])
8, 28 -- with ESMTP id 2009072418:58:33:370130.14691.107404208
8, 28 -- for {Microscopy-at-microscopy.com} ;
8, 28 -- Fri, 24 Jul 2009 18:58:33 +0900 (KST)
8, 28 -- From: "Young-Woon Kim" {youngwk-at-snu.ac.kr}
8, 28 -- To: {Microscopy-at-microscopy.com}
8, 28 -- Subject: Looking for ASID scanning unit for JEOL TEM
8, 28 -- Date: Fri, 24 Jul 2009 18:58:15 +0900
8, 28 -- Message-ID: {025101ca0c45$436b6970$ca423c50$-at-ac.kr}
8, 28 -- MIME-Version: 1.0
8, 28 -- Content-Type: text/plain;
8, 28 -- charset="us-ascii"
8, 28 -- Content-Transfer-Encoding: 7bit
8, 28 -- X-Mailer: Microsoft Office Outlook 12.0
8, 28 -- Thread-Index: AcoMRUM5vSsoYxqbQju9poZJe3m5uw==
8, 28 -- Content-Language: ko
8, 28 -- X-TERRACE-SPAMMARK: NO (SR:31.29)
8, 28 -- (by Terrace)
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Fri, 24 Jul 2009 11:26:03 -0500
Subject: [Microscopy] Administrivia: Testing updated Filter please ignore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

I'm testing the temporary fix to the Email filters
please ignore this message.

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
3, 11 -- From zaluzec-at-microscopy.com Fri Jul 24 11:26:03 2009
3, 11 -- Received: from [172.16.0.112] (msdvpn072.msd.anl.gov [130.202.238.72])
3, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OGQ149005533
3, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 11:26:02 -0500
3, 11 -- Mime-Version: 1.0
3, 11 -- Message-Id: {p06240800c68f90d67d84-at-[172.16.0.112]}
3, 11 -- Date: Fri, 24 Jul 2009 11:26:00 -0500
3, 11 -- To: microscopy-at-microscopy.com
3, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
3, 11 -- Subject: Administrivia: Testing updated Filter please ignore
3, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 24 Jul 2009 11:40:43 -0500
Subject: [Microscopy] Administrivia: Testing 3 updated Filter please ignore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues;

Hopefully, this is the last filter test. Please ignore again.

Nestor

==============================Original Headers==============================
3, 11 -- From zaluzec-at-aaem.amc.anl.gov Fri Jul 24 11:40:43 2009
3, 11 -- Received: from [172.16.0.112] (msdvpn072.msd.anl.gov [130.202.238.72])
3, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OGef8c019940
3, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 11:40:42 -0500
3, 11 -- Mime-Version: 1.0
3, 11 -- Message-Id: {p06240803c68f942d45f4-at-[172.16.0.112]}
3, 11 -- Date: Fri, 24 Jul 2009 11:40:40 -0500
3, 11 -- To: microscopy-at-microscopy.com
3, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
3, 11 -- Subject: Administrivia: Testing 3 updated Filter please ignore
3, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jfish-at-gladstone.ucsf.edu
Date: Fri, 24 Jul 2009 13:54:50 -0500
Subject: [Microscopy] embedding monolayer to section a particular cell (2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Vlad,

I have done this many, many times. The Thermanox Plastic coverslips from
Nunc the best. The catalog number for the round 22mm size is #174977. You
can also order them in many other sizes, both larger and smaller as well as
rectangular shaped. I use them along with ACLAR plastic sheets. You can
cut the ACLAR down to an appropriate size and then after the final resin
infiltration step, just put a drop of resin on the ACLAR and invert your
coverslip over the drop. Polymerize the resin and you are good to go. You
can take the embedded cells to the light microscope and accurately choose
the are that you would like to section, remove just that area and glue it to
a blank resin block. Then section as usual. You can also choose several
areas and layer them like a stack of pancakes and embed them into a resin
block. That way you can section through several layers and obtain cross
sections of the cells, quite handy if you need to see several layers as they
interacted in culture.

I hope this helps to answer your questions.

Good luck and take care,
Jo Dee


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish-at-gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov]
Sent: Thursday, July 23, 2009 8:21 PM
To: jfish-at-gladstone.ucsf.edu

Dear friends and colleagues,

I'll have to do something I've managed to avoid so far: section a cell from
monolayer defined by immunofluorescence under LM and... look for the
centrosome!
The first question I would like to ask, what do you like to use as a
substrate to grow the cells on for such case? I once bought some coverslips
with a kind of grid on them, years ago. Never used them.
They were individually wrapped and I thought were Nunc brand, but I just
looked on the Nunc website and could not find them, nor could I find them in
EM suppliers' catalogs. And no, I don't have them anymore. Never used them
and that was on my earlier job...
Can anyone tell me what they might have been, or perhaps suggest an
alternative? If I don't find a suitable marked substrate, then of course
taking plenty of pictures with the LM camera will do.

My second question is a humble request for any tips on how to best embed so
that I can then locate the cells in the block. I remember someone's
(sorry!..) tip fairly recently where just a thin layer of epoxy was poured
on top of the coverslip - is that what people do?
I remember my lab neighbors some 15 years ago would just put the coverslip
(Nevada-shaped) on the bottom of a round embedding well, pour 3-4 mm resin
on top, and that was it. They were then able to find their cells in the
block allright, although removing the glass was occasionally a problem.

I do have experience embedding monolayers, just never had to section a
particular cell.

Thanks!
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource National Institute of
Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

==============================Original Headers==============================
5, 22 -- From vladislav_speransky-at-nih.gov Thu Jul 23 22:16:47 2009 5, 22 --
Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com
[66.111.4.25])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n6O3GlaM030072
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jul 2009 22:16:47
-0500
5, 22 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
5, 22 -- by out1.messagingengine.com (Postfix) with ESMTP id
EF7843BCAF2;
5, 22 -- Thu, 23 Jul 2009 23:16:46 -0400 (EDT)
5, 22 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
5, 22 -- by compute1.internal (MEProxy); Thu, 23 Jul 2009 23:16:46 -0400
5, 22 -- X-Sasl-enc: RGNsVkSeWrksq8lrY1GI1wNc5KWgIsm2C7HqXjJdwiLE 1248405406
5, 22 -- Received: from [192.168.1.5]
(pool-173-66-201-136.washdc.fios.verizon.net [173.66.201.136])
5, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id
9F2DE3202B;
5, 22 -- Thu, 23 Jul 2009 23:16:46 -0400 (EDT)
5, 22 -- Message-Id: {0E092A1B-3ADA-43EE-92BE-4405D0CAF97D-at-nih.gov}
5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- To:
Microscopy-at-microscopy.com 5, 22 -- Content-Type: text/plain;
charset=US-ASCII; format=flowed; delsp=yes 5, 22 --
Content-Transfer-Encoding: 7bit 5, 22 -- Subject: embedding monolayer to
section a particular cell (2 questions) 5, 22 -- Mime-Version: 1.0 (Apple
Message framework v935.3) 5, 22 -- Date: Thu, 23 Jul 2009 23:16:46 -0400 5,
22 -- X-Mailer: Apple Mail (2.935.3) ==============================End of -
Headers==============================


==============================Original Headers==============================
17, 27 -- From jfish-at-gladstone.ucsf.edu Fri Jul 24 13:54:50 2009
17, 27 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31])
17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OIsnCE007162
17, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 13:54:49 -0500
17, 27 -- X-IronPort-AV: E=Sophos;i="4.43,265,1246863600";
17, 27 -- d="scan'208";a="2370790"
17, 27 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25])
17, 27 -- by gmail2.ucsf.edu with ESMTP; 24 Jul 2009 11:54:48 -0700
17, 27 -- Received: from [169.230.76.4] (HELO JFISH)
17, 27 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10)
17, 27 -- with ESMTP id 567688551; Fri, 24 Jul 2009 11:54:48 -0700
17, 27 -- Reply-To: {jfish-at-gladstone.ucsf.edu}
17, 27 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu}
17, 27 -- To: {vladislav_speransky-at-nih.gov} , {microscopy-at-microscopy.com}
17, 27 -- References: {200907240321.n6O3LN73006403-at-ns.microscopy.com}
17, 27 -- Subject: RE: [Microscopy] embedding monolayer to section a particular cell (2 questions)
17, 27 -- Date: Fri, 24 Jul 2009 11:54:45 -0700
17, 27 -- Organization: J. David Gladstone Institutes
17, 27 -- Message-ID: {9ED0F80DF0E745E0B6957F22E2FD77F1-at-JFISH}
17, 27 -- MIME-Version: 1.0
17, 27 -- Content-Type: text/plain;
17, 27 -- charset="us-ascii"
17, 27 -- Content-Transfer-Encoding: 7bit
17, 27 -- X-Mailer: Microsoft Office Outlook 11
17, 27 -- Thread-Index: AcoMDdMkXn8MqTHZSKKuV0m+sFrTPAAgTbEg
17, 27 -- In-Reply-To: {200907240321.n6O3LN73006403-at-ns.microscopy.com}
17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Fri, 24 Jul 2009 15:24:21 -0500
Subject: [Microscopy] embedding monolayer... THANKS!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To all who responded to my question, on and off the List, my most
sincere thanks!
In the middle of summer, so many knowledgeable people generous with
their time!

I will be just one more question shortly... and promise to contribute
myself more often in the future.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

==============================Original Headers==============================
3, 22 -- From vladislav_speransky-at-nih.gov Fri Jul 24 15:24:20 2009
3, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OKOK0L025257
3, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 15:24:20 -0500
3, 22 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
3, 22 -- by out1.messagingengine.com (Postfix) with ESMTP id 484B93B0D58;
3, 22 -- Fri, 24 Jul 2009 16:24:20 -0400 (EDT)
3, 22 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
3, 22 -- by compute2.internal (MEProxy); Fri, 24 Jul 2009 16:24:20 -0400
3, 22 -- X-Sasl-enc: /EnhY1cDoyaqVM+OGVrb044sGZedd6b0e5SnGRYqORjU 1248467060
3, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
3, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 0107BBFF3;
3, 22 -- Fri, 24 Jul 2009 16:24:19 -0400 (EDT)
3, 22 -- Message-Id: {2848BEE4-39E2-4A27-8FF4-76E92937E2D0-at-nih.gov}
3, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
3, 22 -- To: Microscopy-at-microscopy.com
3, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- Subject: embedding monolayer... THANKS!
3, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
3, 22 -- Date: Fri, 24 Jul 2009 16:24:19 -0400
3, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Fri, 24 Jul 2009 16:00:09 -0500
Subject: [Microscopy] permeabilizing cell culture for AlexaFluorNanogold and Aurion Ultrasmall

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends and colleagues,

This question is for the same project, that of serial sectioning for
the centrosome in *some* of the cells in a cultured monolayer. Those
cells that got transfected, of course ;) The culture is human kidney
epithelium. A protein is expected to be bound in WT but not in the
mutant, and I expect we can only see that by immunolabeling, since the
protein is not very large. This is one case that just calls for pre-
embedding, which has not been my strength (as my then boss Alasdair
Steven put it after one particularly destructive experiment, "The
surgery was successful but the patient is dead"...).

So my question this time is a request to share your favorite way to
permeabilize a *cell culture monolayer* (as opposed to those brain
slices, sorry ;) ), so that IgG molecules can make their way in. I
plan to try both AlexaFluorNanogold and Aurion's Ultrasmall GAR F(ab')
and F(ab')2 as secondary marker. I understand that these probes are
all smaller than a whole IgG molecule.

Now, I have done some homework of course, searched for papers. The
most thorough one I found is Pre-Embedding Immunolabeling for Electron
Microscopy: An Evaluation of Permeabilization Methods and Markers by
Bruno Humbel and coauthors, Microsc. Res. Tech. 42:43 (1998). The
authors seem to have preferred 0.5% TX-100 in PBS 5 minutes, after 30
min pre-fixation with 2%FA-0.02%GA. Pictures look acceptable. I saw
similar protocols in some other sources, including the advice on the
Nanoprobes website, so that's more or less what I plan to do.

I will appreciate your opinions on this matter, particularly any tips
and warnings of perils. As long as damage is not too severe, for the
first run I would like to make sure the Abs can reach the target,
centrosome.

Once all is embedded, and the cells found, and blocks trimmed, I do
have an interesting idea on how to make the serial sectioning part
considerably easier. I'll share it when I get there (and when it
works).

Thanks!
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

==============================Original Headers==============================
7, 22 -- From vladislav_speransky-at-nih.gov Fri Jul 24 16:00:08 2009
7, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OL08jp010531
7, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 16:00:08 -0500
7, 22 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
7, 22 -- by out1.messagingengine.com (Postfix) with ESMTP id 803A13BC786;
7, 22 -- Fri, 24 Jul 2009 17:00:08 -0400 (EDT)
7, 22 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
7, 22 -- by compute1.internal (MEProxy); Fri, 24 Jul 2009 17:00:08 -0400
7, 22 -- X-Sasl-enc: +FjuF61AMNi54OuRxFKEo1HJhwKaemiiJ0vapHqP9I7g 1248469208
7, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
7, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 46356C012;
7, 22 -- Fri, 24 Jul 2009 17:00:08 -0400 (EDT)
7, 22 -- Message-Id: {0E3CDEAD-1155-4E8B-942C-46E8BF3C1FF8-at-nih.gov}
7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
7, 22 -- To: Microscopy-at-microscopy.com
7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- Subject: permeabilizing cell culture for AlexaFluorNanogold and Aurion Ultrasmall
7, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
7, 22 -- Date: Fri, 24 Jul 2009 17:00:08 -0400
7, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: rcguerrero-at-gmail.com
Date: Fri, 24 Jul 2009 16:53:00 -0500
Subject: [Microscopy] Hotel room is available for MandM meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all!
Please contact me if you are still looking for a Hotel to stay in
during MM 2009 in Richmond. I have a reservation at the Commonwealth
Park Suites Hotel - Richmond and I could share that room.
Details below:

Commonwealth Park Suites Hotel - Richmond
901 Bank St
Richmond, VA 23219
USA

Check in: Sun Jul-26-2009
Check out: Thu Jul-30-2009

Room description: Suite
Includes: Full Breakfast
Room type: 2 DOUBLE BEDS

If interested and to discuss the cost please contact me at rcguerr-at-emory.edu

Thanks!
Ricardo

--------------------------------------------------------
Ricardo C. Guerrero-Ferreira, PhD
Emory University School of Medicine
Division of Pediatric Infectious Diseases Emory Children Center
2015 Uppergate Dr NE
Suite 560
Atlanta, GA 30322

Lab: +1 404-727 3752
Cell: +1 575-571 0891
Office: + 1 404-727-0039

==============================Original Headers==============================
8, 31 -- From rcguerrero-at-gmail.com Fri Jul 24 16:52:59 2009
8, 31 -- Received: from mail-bw0-f211.google.com (mail-bw0-f211.google.com [209.85.218.211])
8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OLqxCi026411
8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 16:52:59 -0500
8, 31 -- Received: by bwz7 with SMTP id 7so1619199bwz.18
8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 14:52:58 -0700 (PDT)
8, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 31 -- d=gmail.com; s=gamma;
8, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
8, 31 -- :from:to:content-type:content-transfer-encoding;
8, 31 -- bh=l8D6Le+lectD4/RQyasvG5y3stdwP3h8WrA4osMNDu0=;
8, 31 -- b=WHQYEGlC+rfutpoG9y/UGnLbFI5EEiqh/y9EMAkbwvCzaqETYa77qP7BsiA/8Ea0lh
8, 31 -- bhtH15U/+KiqMbZ9g6mBfPadF+OggkGwQw9tB83paDeBm/wgKeFVoFT7Rw4W4jcrv2Jd
8, 31 -- vGRrQY43Rdnpls/yQFxHu5EGa2JHbN4nHnJus=
8, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 31 -- d=gmail.com; s=gamma;
8, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
8, 31 -- :content-transfer-encoding;
8, 31 -- b=rp/vRl8dJ9OxaPVZQ/4WuV0QVhch3Pb/ELtxxjku3MQ9KZqVhxjk5hjhTycTIM+tX9
8, 31 -- RQgul5rlXLlTtGUvjniXS57B3QVxAfZ4ciPrLJoBz5teOMZ2GEdVJJXdNHXEQWst4Pjg
8, 31 -- IwcL2s6MTx6sNv19ERTis779DfJWctqn6Zt0U=
8, 31 -- MIME-Version: 1.0
8, 31 -- Received: by 10.239.151.82 with SMTP id q18mr402998hbb.22.1248472378326; Fri,
8, 31 -- 24 Jul 2009 14:52:58 -0700 (PDT)
8, 31 -- Date: Fri, 24 Jul 2009 17:52:58 -0400
8, 31 -- Message-ID: {97cbc6d0907241452g677a9db1u3ebc9bab5f00a8bf-at-mail.gmail.com}
8, 31 -- Subject: Hotel room is available for MandM meeting
8, 31 -- From: Ricardo Guerrero {rcguerrero-at-gmail.com}
8, 31 -- To: microscopy-at-microscopy.com
8, 31 -- Content-Type: text/plain; charset=UTF-8
8, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: dma02007-at-mymail.pomona.edu
Date: Fri, 24 Jul 2009 16:54:52 -0500
Subject: [Microscopy] viaWWW: fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dma02007-at-mymail.pomona.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dma02007-at-mymail.pomona.edu
Name: Duncan Ariey

Organization: Pomona College/University of Alaska Anchorage

Title-Subject: [Filtered] fluorescence microscopy

Question: Hello all,

I'm an undergrad at Pomona currently doing research at UAA. I'm
currently tasked with imaging cells in certain conditions via
fluorescence microscopy, and the other day encountered something I
had never seen before. The intention of the experiment was to stain
nuclei with hoechst stain, stain actin with conjugated phalloidin,
and stain cytosolic Rho GEF proteins with primary and secondary
antibodies. The nuclei would then appear in the blue channel, the
actin in the green, and the Rho GEF proteins in the red.

After performing the requisite exposure, fixing, permeablizing,
blocking, and staining, I put the cells under the microscope and was
surprised by two different things. For one, the red channel didn't
stain. It's entirely possible that this was some error on my part and
I'm not too worried about the reasons for this. The second problem
took me a few minutes to notice. While I was viewing the green
channel, actin displayed as it normally does when stained; I could
see the cell outlines, but not much inside the cells. Then, after I
switched to the blue channel to look at nuclei for a few minutes and
switched back to green, the nuclei were flourescing green, and much
brighter than the actin at the membranes of the cells. If I moved to
a new area of the slide, or even to a new slide entirely, the process
repeated itself.

Does anyone have any thoughts as to what could have caused this? I've
been doing this staining process for several weeks now and this is
the first instance. The only new procedure introduced this time was
that I blocked with Invitrogen Image-iT FX signal enhancer in
addition to growth media containing 10% FBS. We also recently found
out that some of our cell cultures were infected with bacteria, so
that may have had a hand in it. Is there any kind of foreign particle
that could have been introduced to the culture that absorbs light at
the blue excitation wavelength and emits it at the green emission
wavelength?

Any help would be most appreciated,

Duncan Ariey

Login Host: 137.229.143.184
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Jul 24 16:54:52 2009
11, 11 -- Received: from [172.16.0.112] (msdvpn072.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OLsoja029516
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 16:54:51 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c68fde12828d-at-[172.16.0.112]}
11, 11 -- Date: Fri, 24 Jul 2009 17:54:49 -0400
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dma02007-at-mymail.pomona.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: fluorescence microscopy
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kun.li-at-kaust.edu.sa
Date: Sat, 25 Jul 2009 15:00:39 -0500
Subject: [Microscopy] viaWWW: EMS and Desktop Microscopist software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kun.li-at-kaust.edu.sa as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kun.li-at-kaust.edu.sa
Name: kun li

Organization: KAUST

Title-Subject: [Filtered] EMS and Desktop Microscopist software

Question: Dear Listers,

We are planning to but a few sets of EMS and Desktop Microscopist
software. Does anyone of you know how to make the purchase ( how to
contact the software owner)?

Regards,

Simon
KAUST
Imaging Core Lab

Login Host: 78.93.149.23
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Sat Jul 25 15:00:39 2009
9, 11 -- Received: from [12.50.200.53] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6PK0c8L028949
9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 25 Jul 2009 15:00:39 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c69114d3b4b5-at-[12.50.200.53]}
9, 11 -- Date: Sat, 25 Jul 2009 16:00:37 -0400
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: kun.li-at-kaust.edu.sa (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: EMS and Desktop Microscopist software
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Mon, 27 Jul 2009 02:24:03 -0500
Subject: [Microscopy] permeabilizing cell culture for AlexaFluorNanogold and Aurion Ultrasmall

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Vlad!

In my experience the permeabilization conditions should be optimized for each cell line!
(which is quite common in cell biology, isn't it?)
Some are more sensitive to detergents, some are indestructible! :-)
Triton X-100 worked well for me too. As you probably guessed you have to play with the concentrations, time and temperature.
Sometimes I got best results at RT but then the time must be very short!
If I remember well the centrosome is in cytoplasm :-))) which is good because permeabilizing the nucleus requires stringent conditions.
If you like to try perilous things you may try very low concentrations of detergent along with your Ab. You may make lots of victims, but cells in culture do not often complain (never been sued for the millions of victims I make daily).
Just use a common labeling like actin during the optimization trials.

Best luck,

Stephane


----- Original Message ----
X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov}
To: nizets2-at-yahoo.com
Sent: Friday, July 24, 2009 11:03:41 PM

Dear friends and colleagues,

This question is for the same project, that of serial sectioning for 
the centrosome in *some* of the cells in a cultured monolayer. Those 
cells that got transfected, of course ;) The culture is human kidney 
epithelium. A protein is expected to be bound in WT but not in the 
mutant, and I expect we can only see that by immunolabeling, since the 
protein is not very large. This is one case that just calls for pre-
embedding, which has not been my strength (as my then boss Alasdair 
Steven put it after one particularly destructive experiment, "The 
surgery was successful but the patient is dead"...).

So my question this time is a request to share your favorite way to 
permeabilize a *cell culture monolayer* (as opposed to those brain 
slices, sorry ;) ), so that IgG molecules can make their way in. I 
plan to try both AlexaFluorNanogold and Aurion's Ultrasmall GAR F(ab') 
and F(ab')2 as secondary marker. I understand that these probes are 
all smaller than a whole IgG molecule.

Now, I have done some homework of course, searched for papers. The 
most thorough one I found is Pre-Embedding Immunolabeling for Electron 
Microscopy: An Evaluation of Permeabilization Methods and Markers by 
Bruno Humbel and coauthors, Microsc. Res. Tech. 42:43 (1998). The 
authors seem to have preferred 0.5% TX-100 in PBS 5 minutes, after 30 
min pre-fixation with 2%FA-0.02%GA. Pictures look acceptable. I saw 
similar protocols in some other sources, including the advice on the 
Nanoprobes website, so that's more or less what I plan to do.

I will appreciate your opinions on this matter, particularly any tips 
and warnings of perils. As long as damage is not too severe, for the 
first run I would like to make sure the Abs can reach the target, 
centrosome.

Once all is embedded, and the cells found, and blocks trimmed, I do 
have an interesting idea on how to make the serial sectioning part 
considerably easier.  I'll share it when I get there (and when it 
works).

Thanks!
Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

==============================Original Headers==============================
7, 22 -- From vladislav_speransky-at-nih.gov Fri Jul 24 16:00:08 2009
7, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
7, 22 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6OL08jp010531
7, 22 --     for {Microscopy-at-microscopy.com} ; Fri, 24 Jul 2009 16:00:08 -0500
7, 22 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
7, 22 --     by out1.messagingengine.com (Postfix) with ESMTP id 803A13BC786;
7, 22 --     Fri, 24 Jul 2009 17:00:08 -0400 (EDT)
7, 22 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
7, 22 --  by compute1.internal (MEProxy); Fri, 24 Jul 2009 17:00:08 -0400
7, 22 -- X-Sasl-enc: +FjuF61AMNi54OuRxFKEo1HJhwKaemiiJ0vapHqP9I7g 1248469208
7, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
7, 22 --     by mail.messagingengine.com (Postfix) with ESMTPA id 46356C012;
7, 22 --     Fri, 24 Jul 2009 17:00:08 -0400 (EDT)
7, 22 -- Message-Id: {0E3CDEAD-1155-4E8B-942C-46E8BF3C1FF8-at-nih.gov}
7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
7, 22 -- To: Microscopy-at-microscopy.com
7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- Subject: permeabilizing cell culture for AlexaFluorNanogold and Aurion Ultrasmall
7, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3)
7, 22 -- Date: Fri, 24 Jul 2009 17:00:08 -0400
7, 22 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================






==============================Original Headers==============================
23, 25 -- From nizets2-at-yahoo.com Mon Jul 27 02:24:03 2009
23, 25 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6R7O2jb014619
23, 25 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 02:24:03 -0500
23, 25 -- Received: (qmail 79086 invoked by uid 60001); 27 Jul 2009 07:24:02 -0000
23, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1248679442; bh=zMHMWj2WmulGMUR0+Hf8TwA11SfMeWFx2YuIaAm8HlE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=ZxaCeZMr+hmPS2yA76IbazgnkfKsbY3PfSEHJdh3x/NTEUt3kaUkI2YaXVIw3QxNnls2QKqThF9MJM7MMSHADWyZH6F1gEbmmqMxVzBOpdnbgV0F978ie9ohXxAHC8xZpta8kYDCWJ2JZt1Qh04CF9m3HSaqI55zzkR8yt96Crw=
23, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 25 -- s=s1024; d=yahoo.com;
23, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 25 -- b=40fZOZg/677v8iCZeaiAGje7tifn02b2EmMKPPAKEmECKAohiYsuCWQGESz4rv/b9Wumy6e8WiELF7+RGoOaahSW7faCzl4mxgCmvgWg9Xqxtz5LLURrMSi4ClU/pe6wS20XnieLo0gkYyRTajAH+EbfGQhxz/m8A4ecqET/vXI=;
23, 25 -- Message-ID: {74693.78382.qm-at-web110805.mail.gq1.yahoo.com}
23, 25 -- X-YMail-OSG: auU3eBcVM1nwsI0g2GywJO_g.dq8hhsSz1FENXPtJ_9dn3gJDQ2.adLMIEEJrL00n6pL0.t515nZWiCrbAPFlvezDPnVGi12NJAH84.LwdpF2zbk53IUcidFwDD0i66gZESxnZhjc70NqmfP_QTs1CN3EBq4fg0onqnIWJJmn7ruknRMeGD3nYrIQrzdUAQJ6AbhA.4ldbJ9Ip.xDSmY1pd2wXRTi3dS34Dmanaq5N.9Sa3btb3.AMkQtAcXAXlP5GfO_0S5x0.5QXctf.NK9GGE9ncBCD9pzNiT9jqTzruaaqS2VWY86QtlcTvd8S7711KXrSfiVc5qi6fUcF1gMBQPIpaJvOBFHVZlDysNYz8-
23, 25 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Mon, 27 Jul 2009 00:24:01 PDT
23, 25 -- X-Mailer: YahooMailRC/1358.22 YahooMailWebService/0.7.289.15
23, 25 -- References: {200907242103.n6OL3fo1016916-at-ns.microscopy.com}
23, 25 -- Date: Mon, 27 Jul 2009 00:24:01 -0700 (PDT)
23, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 25 -- Subject: Re: [Microscopy] permeabilizing cell culture for AlexaFluorNanogold and Aurion Ultrasmall
23, 25 -- To: vladislav_speransky-at-nih.gov
23, 25 -- Cc: microscopy-at-microscopy.com
23, 25 -- In-Reply-To: {200907242103.n6OL3fo1016916-at-ns.microscopy.com}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain; charset=iso-8859-1
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6R7O2jb014619
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Mon, 27 Jul 2009 03:13:04 -0500
Subject: [Microscopy] LM: Microscope repair service.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for a good service company in the south Florida area,
preferably in Miami. I have a Leica compound scope that needs
cleaning, and would like recommendations on a firm that would be able
to do it. Any comments on past experiences as well as commercial
replies are welcome off-list.

Thank you,

Justin A. Kraft

Sent from my iPhone

==============================Original Headers==============================
4, 40 -- From kraftpiano-at-gmail.com Mon Jul 27 03:12:54 2009
4, 40 -- Received: from mail-yx0-f190.google.com (mail-yx0-f190.google.com [209.85.210.190])
4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6R8Crxg030319
4, 40 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 03:12:54 -0500
4, 40 -- Received: by yxe28 with SMTP id 28so5699969yxe.10
4, 40 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 01:12:52 -0700 (PDT)
4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 40 -- d=gmail.com; s=gamma;
4, 40 -- h=domainkey-signature:received:received:message-id:from:to
4, 40 -- :content-type:content-transfer-encoding:x-mailer:mime-version
4, 40 -- :subject:date;
4, 40 -- bh=uJOa63aeYdM3t/WgHAu98pLIFPlEQ294Bn7QhpSlO/o=;
4, 40 -- b=uS0b/wMxwxFmJMQFHw4ay0L4vu1TvantDaHU+EyZlYB5BtrOL5JOfYlwuXdF/YWVIl
4, 40 -- HSK9JV76cZxtFyZlBTMYM90YQtdjQL1POcXNXy7Qm4sUEsf7u72tCeUdXZnZmAFykT+D
4, 40 -- gBOF1nPY629j6Z5Ol+U0WVJd/0yAyKzcA6XCc=
4, 40 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 40 -- d=gmail.com; s=gamma;
4, 40 -- h=message-id:from:to:content-type:content-transfer-encoding:x-mailer
4, 40 -- :mime-version:subject:date;
4, 40 -- b=KThOz/LN63unpTWvZSx1DG9aqf8PG7GGdG5yhcel61LlfKFi4Aho6kjuR0ZJ0hnGWG
4, 40 -- 41RSizZYDnvvyAS5rfjLe4o47+R/YY2mvOVcIN5uYEDq9ACJ5YYjFdtp+vyHJxunGjQN
4, 40 -- OKpD0Yyp0dW6LjRjwPqTB7b7mV2SNjxvQKIq4=
4, 40 -- Received: by 10.100.166.7 with SMTP id o7mr7868914ane.90.1248682372259;
4, 40 -- Mon, 27 Jul 2009 01:12:52 -0700 (PDT)
4, 40 -- Received: from ?10.19.235.130? ([166.195.185.233])
4, 40 -- by mx.google.com with ESMTPS id b7sm15899294ana.17.2009.07.27.01.12.50
4, 40 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 40 -- Mon, 27 Jul 2009 01:12:51 -0700 (PDT)
4, 40 -- Message-Id: {72B793F2-55B6-4DE7-8F5E-92D3D6995770-at-gmail.com}
4, 40 -- From: "Justin A. Kraft" {kraftpiano-at-gmail.com}
4, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
4, 40 -- Content-Type: text/plain;
4, 40 -- charset=us-ascii;
4, 40 -- format=flowed;
4, 40 -- delsp=yes
4, 40 -- Content-Transfer-Encoding: 7bit
4, 40 -- X-Mailer: iPhone Mail (7A341)
4, 40 -- Mime-Version: 1.0 (iPhone Mail 7A341)
4, 40 -- Subject: LM: Microscope repair service.
4, 40 -- Date: Mon, 27 Jul 2009 04:12:39 -0400
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Mon, 27 Jul 2009 06:58:49 -0500
Subject: [Microscopy] Resolution: Oxford Inca error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,

Just a note to thank all the listers who helped out with my EDS problem
(especially Warren Straszheim). Happily a zero-cost solution turned up,
and the system has hummed along all weekend without any errors. Rather
than take up space here describing a solution that is most likely
peculiar to Inca systems, please contact me off list if you happen to
come across the same error message.

A million thanks again - makes we wonder if I shouldn't be spending
service contract money on beer and pizza for the listers instead!

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
8, 18 -- From jehrman-at-mta.ca Mon Jul 27 06:58:48 2009
8, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.204])
8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6RBwlAE023281
8, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 06:58:48 -0500
8, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:59860)
8, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
8, 18 -- with esmtp id 1MVOrj-0007N2-0R (Exim 4.69) for Microscopy-at-microscopy.com
8, 18 -- (return-path {jehrman-at-mta.ca} ); Mon, 27 Jul 2009 08:59:39 -0300
8, 18 -- Message-ID: {4A6D94D3.3070307-at-mta.ca}
8, 18 -- Date: Mon, 27 Jul 2009 08:51:47 -0300
8, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
8, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
8, 18 -- MIME-Version: 1.0
8, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
8, 18 -- Subject: Resolution: Oxford Inca error
8, 18 -- X-Enigmail-Version: 0.96.0
8, 18 -- Content-Type: text/plain; charset=ISO-8859-1
8, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: dcristofori-at-unive.it
Date: Mon, 27 Jul 2009 08:09:29 -0500
Subject: [Microscopy] Re: viaWWW: EMS and Desktop Microscopist software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hesitation is a sign of weakness.
Be strong! :-))

Stephane




----- Original Message ----
X-from: "jehrman-at-mta.ca" {jehrman-at-mta.ca}
To: nizets2-at-yahoo.com
Sent: Monday, July 27, 2009 2:07:51 PM

Hi Simon
As for EMS I guess you mean the old DOS software by Prof. Stadelmann,
which was upgraded to a java version called jems.
You can find all the information you need at this page:

http://cimewww.epfl.ch/people/stadelmann/jemsWebSite/jems.html



We don't use Desktop Microscopist... but try this:


Contacting Virtual Laboratories

For sales, Demos, and general questions, please contact Virtual
Laboratories at:

Virtual Laboratories
P.O. Box 14266
Albuquerque, NM 87191-4266

Phone: (505) 828-1640
Fax: (505) 822-9759
URL: http://www.rt66.com/~virtlabs/

Or email us at VirtLabs-at-rt66.com



Regards

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive.it

Universita' Ca' Foscari Venezia
Dipartimento di Chimica Fisica
Lab. di Scienza e Tecnologia dei Materiali
Via Torino, 155b
I-30172 Mestre (VE)
Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

kun.li-at-kaust.edu.sa ha scritto:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both kun.li-at-kaust.edu.sa as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: kun.li-at-kaust.edu.sa
} Name: kun li
}
} Organization: KAUST
}
} Title-Subject: [Filtered] EMS and Desktop Microscopist software
}
} Question: Dear Listers,
}
} We are planning to but a few sets of EMS and Desktop Microscopist
} software. Does anyone of you know how to make the purchase ( how to
} contact the software owner)?
}
} Regards,
}
} Simon
} KAUST
} Imaging Core Lab
}
} Login Host: 78.93.149.23
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 11 -- From zaluzec-at-microscopy.com Sat Jul 25 15:00:39 2009
} 9, 11 -- Received: from [12.50.200.53] (msdvpn072.msd.anl.gov [130.202.238.72])
} 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6PK0c8L028949
} 9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 25 Jul 2009 15:00:39 -0500
} 9, 11 -- Mime-Version: 1.0
} 9, 11 -- Message-Id: {p06240801c69114d3b4b5-at-[12.50.200.53]}
} 9, 11 -- Date: Sat, 25 Jul 2009 16:00:37 -0400
} 9, 11 -- To: microscopy-at-microscopy.com
} 9, 11 -- From: kun.li-at-kaust.edu.sa (by way of MicroscopyListserver)
} 9, 11 -- Subject: viaWWW: EMS and Desktop Microscopist software
} 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


==============================Original Headers==============================
21, 27 -- From dcristofori-at-unive.it Mon Jul 27 08:09:27 2009
21, 27 -- Received: from zeus.unive.it (zeus.unive.it [157.138.1.81])
21, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6RD9Pvm021956
21, 27 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 08:09:27 -0500
21, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1])
21, 27 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n6RD9NoZ002978;
21, 27 -- Mon, 27 Jul 2009 15:09:23 +0200
21, 27 -- Received: from zeus.unive.it ([127.0.0.1])
21, 27 -- by localhost (zeus.unive.it [127.0.0.1]) (amavisd-new, port 10024)
21, 27 -- with LMTP id agdOzJ58i8fV; Mon, 27 Jul 2009 15:09:22 +0200 (CEST)
21, 27 -- Received: from helios.unive.it (helios.unive.it [157.138.8.4])
21, 27 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n6RD9K4Z002964;
21, 27 -- Mon, 27 Jul 2009 15:09:20 +0200
21, 27 -- Received: from [127.0.0.1] (ferroni.dcf.unive.it [157.138.23.68])
21, 27 -- by helios.unive.it (8.13.8/8.13.8) with ESMTP id n6RD9jdV023466;
21, 27 -- Mon, 27 Jul 2009 15:09:58 +0200
21, 27 -- Message-ID: {4A6DA936.4060205-at-unive.it}
21, 27 -- Date: Mon, 27 Jul 2009 15:18:46 +0200
21, 27 -- From: Davide Cristofori {dcristofori-at-unive.it}
21, 27 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
21, 27 -- MIME-Version: 1.0
21, 27 -- To: kun.li-at-kaust.edu.sa, microscopy-at-microscopy.com
21, 27 -- Subject: Re: [Microscopy] viaWWW: EMS and Desktop Microscopist software
21, 27 -- References: {200907252007.n6PK7XPf006229-at-ns.microscopy.com}
21, 27 -- In-Reply-To: {200907252007.n6PK7XPf006229-at-ns.microscopy.com}
21, 27 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
21, 27 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: xin-at-magnet.fsu.edu
Date: Mon, 27 Jul 2009 16:45:20 -0500
Subject: [Microscopy] viaWWW: Advanced Analytical TEM Laboratory Support Scientist at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both xin-at-magnet.fsu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: xin-at-magnet.fsu.edu
Name: Yan Xin

Organization: Florida State University

Title-Subject: [Filtered] job opening

Question: Advanced Analytical TEM Laboratory
Support Scientist at Florida State University

Background:
Florida State University is building a world
class materials characterization facility which
will include a new generation Cs corrected sub-‰
resolution, cold field emission, analytical
TEM/STEM (JEOL JEM-ARM200F). This is a key
component of a developing Materials
Characterization Center which is fundamental to
the success of a multi-departmental initiative in
the Growth, Processing, and Characterization of
Advanced Materials supported by FSUís Pathways of
Excellence Initiative (http://pathways.fsu.edu/).
Strong parallel support for the TEM facility
comes from the Departments of Chemistry, Physics,
Mechanical Engineering, and Industrial &
Manufacturing Engineering, the High-Performance
Materials Institute, the Applied
Superconductivity Center and National High
Magnetic Field Laboratory. The TEM laboratory is
located in the National High Field Laboratory in
Tallahassee, Florida. The major equipment
currently includes a JEM-2011 LaB6 TEM, a Gatan
PIPS, and a Fischone ion mill. There are also
Zeiss 1540 EsB and 1540 XB FESEM/FIB facilities
located in the adjacent Shaw Building that are
similarly available to trained users and
supported for general access.
Functions of the TEM support scientist
Responsibilities include:
* Interacting, assisting and training users for
routine operation of the TEM/STEM microscopes and
related sample preparation equipment.
* Routine maintenance (major equipment is under
service contract) and essential repair of the
microscopes and related sample preparation
equipment.
* Management of routine operation, including
logs, book keeping, billing, maintenance record,
inventory, and consumables purchase.
* Make recommendations and assisting in the purchase of new equipment.
* Participate in the microscopy community and related research activities
* Prepare monthly and annual reports concerning
the TEM Laboratory and participate required
review meetings
* Provide outreach and tours for the general public.
Qualifications, experience and skills:
* Advanced University degree (Masterís or higher)
and demonstrated experience in instrumentation
support with at least four years of relevant
experience. Applicants with experience working in
a multi-user facility are especially encouraged.
* Proven knowledge and experience with electron
microscopes (TEM experience is preferred).
* Good management skills relevant to a multi-user
environment and good communication and
interpersonal skills.

Salary Range: Salary range up to $60,000
commensurate with qualifications and experience.

Florida State University is an equal opportunity
employer providing excellent compensation and
benefit packages.
Contact information:
Prof. David Larbalestier
253 Shaw Building
2031 E. Paul Dirac Dr., Tallahassee, FL 32310
Email: larbalestier-at-asc.magnet.fsu.edu




Login Host: 146.201.233.66
---------------------------------------------------------------------------


==============================Original Headers==============================
13, 14 -- From zaluzec-at-microscopy.com Mon Jul 27 16:45:20 2009
13, 14 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov [130.202.238.72])
13, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6RLjIkW028867
13, 14 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2009 16:45:19 -0500
13, 14 -- Mime-Version: 1.0
13, 14 -- Message-Id: {p06240801c693d0568ccd-at-[172.16.0.101]}
13, 14 -- Date: Mon, 27 Jul 2009 17:45:16 -0400
13, 14 -- To: microscopy-at-microscopy.com
13, 14 -- From: xin-at-magnet.fsu.edu (by way of MicroscopyListserver)
13, 14 -- Subject: viaWWW: Advanced Analytical TEM Laboratory Support Scientist at
13, 14 -- Florida State University
13, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
13, 14 -- Content-Transfer-Encoding: 8bit
13, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6RLjIkW028867
==============================End of - Headers==============================




From: daveparm-at-yahoo.com
Date: Wed, 29 Jul 2009 08:24:18 -0500
Subject: [Microscopy] viaWWW: EDAX Genesis system on a TEM/STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both daveparm-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: daveparm-at-yahoo.com
Name: David Parmiter

Organization: SAIC

Title-Subject: [Filtered] EDAX Genesis

Question: Hi all -

I'd like to know anyone's experience with an EDAX EDS system on a
TEM/STEM using their newest Genesis software on a biological sample.

Specifically, I'd like to know if the program is easy-to-use, how
long does acquiring a standard map take, what kind of counts/second
do you get, and how accurate do you think the software is in judging
elemental composition?

Thanks kindly in advance!

- Dave Parmiter

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Jul 29 08:24:17 2009
10, 11 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov [130.202.238.72])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6TDOFjQ028540
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jul 2009 08:24:17 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c695fdcab269-at-[172.16.0.101]}
10, 11 -- Date: Wed, 29 Jul 2009 09:24:12 -0400
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: daveparm-at-yahoo.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: EDAX Genesis system on a TEM/STEM
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Wed, 29 Jul 2009 11:22:19 -0500
Subject: [Microscopy] Re: viaWWW: EDAX Genesis system on a TEM/STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI Dave
Questions like:
} how long does acquiring a standard map take, what kind of counts/
} second
} do you get
Are TEM (electron source and mode of operation) and sample specific
and can not easily be compared. Contact EDAX and schedule a demo with
your samples and on TEM/STEM that you have or would purchase.

Kind regards,
Roseann

Roseann Csencsits, PhD
Scientist in Charge - Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548







On Jul 29, 2009, at 6:50 AM, daveparm-at-yahoo.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both daveparm-at-yahoo.com as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: daveparm-at-yahoo.com
} Name: David Parmiter
}
} Organization: SAIC
}
} Title-Subject: [Filtered] EDAX Genesis
}
} Question: Hi all -
}
} I'd like to know anyone's experience with an EDAX EDS system on a
} TEM/STEM using their newest Genesis software on a biological sample.
}
} Specifically, I'd like to know if the program is easy-to-use, how
} long does acquiring a standard map take, what kind of counts/second
} do you get, and how accurate do you think the software is in judging
} elemental composition?
}
} Thanks kindly in advance!
}
} - Dave Parmiter
}
}

==============================Original Headers==============================
11, 23 -- From RCsencsits-at-lbl.gov Wed Jul 29 11:22:19 2009
11, 23 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6TGMJGc025053
11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jul 2009 11:22:19 -0500
11, 23 -- X-Ironport-SBRS: None
11, 23 -- X-IronPort-Anti-Spam-Filtered: true
11, 23 -- X-IronPort-Anti-Spam-Result: AnYHAM4TcEqD8yP2/2dsb2JhbACCFI0KtUwJj36CS4FGBQ
11, 23 -- X-IronPort-AV: E=Sophos;i="4.43,289,1246863600";
11, 23 -- d="scan'208";a="101399565"
11, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov ([131.243.35.246])
11, 23 -- by ironport2.lbl.gov with ESMTP/TLS/AES128-SHA; 29 Jul 2009 09:22:18 -0700
11, 23 -- Cc: Microscopy-at-microscopy.com
11, 23 -- Message-Id: {B126E1A1-0085-4120-A531-BAD5422573C6-at-lbl.gov}
11, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
11, 23 -- To: daveparm-at-yahoo.com
11, 23 -- In-Reply-To: {200907291350.n6TDoAoU013112-at-ns.microscopy.com}
11, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 23 -- Content-Transfer-Encoding: 7bit
11, 23 -- Mime-Version: 1.0 (Apple Message framework v935.3)
11, 23 -- Subject: Re: [Microscopy] viaWWW: EDAX Genesis system on a TEM/STEM
11, 23 -- Date: Wed, 29 Jul 2009 09:22:16 -0700
11, 23 -- References: {200907291350.n6TDoAoU013112-at-ns.microscopy.com}
11, 23 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Wed, 29 Jul 2009 22:05:38 -0500
Subject: [Microscopy] Digital micrograph and iTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We have recently installed and new camera on our TEM and it came with the iTEM software from Olympus-SIS. Up until then we had been using Digital Micrograph (embarrassingly, version 2.5 on the Mac's). While we can save the DM images as 8 bit TIFF files I was wondering if anyone had tried successfully to import the original DM file into iTEM.

Using the define file type dialogue box in iTEM I can almost get them correctly imported, but there is some pixel wrap around and a small amount of noise inserted at the bottom of the image. As our TEM couldn't interface with DM there is no magnification information to be imported etc. If the annotation layers in the DM image like the micron marker could also be imported that would be very useful.

Any assistance would be greatly appreciated.

Cheers

Colin Veitch

Electron Microscopist
CSIRO Materials Science and Engineering, Geelong Laboratory
PO Box 21, BELMONT, Vic. 3216. Australia.
colin.veitch-at-csiro.au
http://www.tft.csiro.au

Tel:       +61 (0) 3 5246 4000  
Mobile:  0438 538 475
Fax:      +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.



==============================Original Headers==============================
11, 46 -- From prvs=4555220e5=Colin.Veitch-at-csiro.au Wed Jul 29 22:05:38 2009
11, 46 -- Received: from vic-MTAout3.csiro.au (vic-MTAout3.csiro.au [150.229.64.39])
11, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6U35bLa032705
11, 46 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jul 2009 22:05:37 -0500
11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
11, 46 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt;
11, 46 -- s=email; t=1248923137; x=1280459137;
11, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
11, 46 -- mime-version:content-transfer-encoding:content-id:
11, 46 -- content-description:resent-date:resent-from:resent-sender:
11, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
11, 46 -- references:list-id:list-help:list-unsubscribe:
11, 46 -- list-subscribe:list-post:list-owner:list-archive;
11, 46 -- z=From:=20 {Colin.Veitch-at-csiro.au} |Subject:=20Digital=20mic
11, 46 -- rograph=20and=20iTEM|Date:=20Thu,=2030=20Jul=202009=2013:
11, 46 -- 05:20=20+1000|Message-ID:=20 {4AA5DD81F68A934F8C80DA2BF125
11, 46 -- 163D333F36D2-at-EXVIC-MBX02.nexus.csiro.au} |To:=20 {microscop
11, 46 -- y-at-microscopy.com} |MIME-Version:=201.0
11, 46 -- |Content-Transfer-Encoding:=20quoted-printable;
11, 46 -- bh=jvDDcxWtucIXwe1b+EYF1+zMukp/4UN+UqFwDTm7cEQ=;
11, 46 -- b=TihugvWax58HFtIhFvUOF8w4IDjzOZx35x5tGEz9HFSnShHF3UlO5aYw
11, 46 -- Sv/hKnkv4V6PWIYkM08Mm5Vy6UxTKFPdnWgOCQ45LpJ8N+o6qGJgSixsn
11, 46 -- qhjaysw6+o3iRbm;
11, 46 -- X-IronPort-AV: E=Sophos;i="4.43,292,1246802400";
11, 46 -- d="scan'208";a="19320014"
11, 46 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127])
11, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 30 Jul 2009 13:05:35 +1000
11, 46 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by
11, 46 -- exvic-htca02.nexus.csiro.au ([138.194.81.127]) with mapi; Thu, 30 Jul 2009
11, 46 -- 13:05:21 +1000
11, 46 -- From: {Colin.Veitch-at-csiro.au}
11, 46 -- To: {microscopy-at-microscopy.com}
11, 46 -- Date: Thu, 30 Jul 2009 13:05:20 +1000
11, 46 -- Subject: Digital micrograph and iTEM
11, 46 -- Thread-Topic: Digital micrograph and iTEM
11, 46 -- Thread-Index: AcoQwpLPho4vTY2wSsWmslYHCm23Hg==
11, 46 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D333F36D2-at-EXVIC-MBX02.nexus.csiro.au}
11, 46 -- Accept-Language: en-US, en-AU
11, 46 -- Content-Language: en-US
11, 46 -- X-MS-Has-Attach:
11, 46 -- X-MS-TNEF-Correlator:
11, 46 -- acceptlanguage: en-US, en-AU
11, 46 -- Content-Type: text/plain; charset="iso-8859-1"
11, 46 -- MIME-Version: 1.0
11, 46 -- Content-Transfer-Encoding: 8bit
11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6U35bLa032705
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 29 Jul 2009 23:32:06 -0500
Subject: [Microscopy] Re: viaWWW: EDAX Genesis system on a TEM/STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't think that EDAX's latest Genesis s/w is the
biggest concern. Their current Genesis is very good.
I use it. The main issue I think is the detector.

In the past, it was a Si(Li) Dewar detector for everyone.
IMO, these are history. Every maker now offers a SDD.
This third or fourth generation SDD product line is awesome.
What happened is that the SDD chip makers moved the FET
from the center of the detector to the outside of the
total chip, resulting in a more tear drop appearance.

As I understand it, all EDS makers are getting the detector
chips from the same source (correction is appreciated). Therefore,
the software becomes a big issue as well as how the makers
integrate the detector chip into a detector unit.

I see high cps advertisements with flat line resolution.
I do not believe this. Furthermore, your specimen is going
to have a big effect on actual results. Is it coated or not?
Wet, dry, whatever. I find that the detectors can do high
cps but cannot be processed {30% DT by the DPP. Someone is
going to figure out how to divide and conquer DPP using multi-cell
processing. At present, they use mostly FPGAs. This does not
exclude them. A multi-cell processor will make a huge difference, IMO.

If you think that you can get 1M cps at 30% DT, good luck.
Plus, your sample/specimen will have lots to say about your
results. This is a huge variable.

I think Genesis is very good and easy to use. But it is not
simple...nor are the other makers' GUIs. But, once you get
used to it, no change to another brand...IMO. Delta cost is
an obvious topic. If you ever go with EBSD, EDAX has that
very nicely covered.

OK...disclaimer...I am a user of EDAX products. So there.

gary g.


At 06:29 AM 7/29/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
14, 20 -- From gary-at-gaugler.com Wed Jul 29 23:32:06 2009
14, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6U4W6tL017073
14, 20 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jul 2009 23:32:06 -0500
14, 20 -- Message-Id: {200907300432.n6U4W6tL017073-at-ns.microscopy.com}
14, 20 -- Received: (qmail 5816 invoked from network); 29 Jul 2009 21:56:12 -0700
14, 20 -- Received: by simscan 1.1.0 ppid: 5810, pid: 5812, t: 0.1072s
14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
14, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
14, 20 -- by smtp1 with SMTP; 29 Jul 2009 21:56:12 -0700
14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 20 -- Date: Wed, 29 Jul 2009 21:31:27 -0700
14, 20 -- To: daveparm-at-yahoo.com
14, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
14, 20 -- Subject: Re: [Microscopy] viaWWW: EDAX Genesis system on a TEM/STEM
14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
14, 20 -- In-Reply-To: {200907291329.n6TDTcHE031623-at-ns.microscopy.com}
14, 20 -- References: {200907291329.n6TDTcHE031623-at-ns.microscopy.com}
14, 20 -- Mime-Version: 1.0
14, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 30 Jul 2009 00:06:22 -0500
Subject: [Microscopy] Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Why post anything to the list?

I get 10-15 out of office crap.

Ridiculous.

Thanks...I'll get another 15 OOF msgs to this one.

gary g.


==============================Original Headers==============================
6, 17 -- From gary-at-gaugler.com Thu Jul 30 00:06:22 2009
6, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6U56LHq032455
6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 00:06:22 -0500
6, 17 -- Message-Id: {200907300506.n6U56LHq032455-at-ns.microscopy.com}
6, 17 -- Received: (qmail 16984 invoked from network); 29 Jul 2009 22:30:28 -0700
6, 17 -- Received: by simscan 1.1.0 ppid: 16979, pid: 16981, t: 0.1339s
6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
6, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
6, 17 -- by smtp1 with SMTP; 29 Jul 2009 22:30:28 -0700
6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
6, 17 -- Date: Wed, 29 Jul 2009 22:05:43 -0700
6, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
6, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
6, 17 -- Subject: Out of office BS
6, 17 -- Mime-Version: 1.0
6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gprodan-at-univ-ovidius.ro
Date: Thu, 30 Jul 2009 05:17:52 -0500
Subject: [Microscopy] Digital micrograph and iTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I work with iTEM and recently I have opportunities to work on Tecnai F30
connected to Gatan Microscope Suite package. The format of files was DM3,
and I have no problem to import them in iTEM (with all informations:
magnification, scale, HT). I don't know what happened if the format is old
one ( like DM2 or else) .



Google for DM script to convert old format to new one (large freeware
scripts database on Graz University of Technology web).



Good luck

Gabi Prodan

PhD Student


==============================Original Headers==============================
9, 19 -- From gprodan-at-univ-ovidius.ro Thu Jul 30 05:17:52 2009
9, 19 -- Received: from univ-ovidius.ro (mail.univ-ovidius.ro [81.196.74.53])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UAHpAY031170
9, 19 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 05:17:52 -0500
9, 19 -- Received: from emsrv ([81.196.74.130]) by univ-ovidius.ro with Microsoft SMTPSVC(6.0.3790.3959);
9, 19 -- Thu, 30 Jul 2009 13:18:14 +0300
9, 19 -- From: "gabi prodan" {gprodan-at-univ-ovidius.ro}
9, 19 -- To: {microscopy-at-microscopy.com}
9, 19 -- Subject: Digital micrograph and iTEM
9, 19 -- Date: Thu, 30 Jul 2009 13:17:38 +0300
9, 19 -- Message-ID: {000001ca10fe$f6cfc6b0$e46f5410$-at-ro}
9, 19 -- MIME-Version: 1.0
9, 19 -- Content-Type: text/plain;
9, 19 -- charset="us-ascii"
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- X-Mailer: Microsoft Office Outlook 12.0
9, 19 -- Thread-Index: AcoQ/vagUjTL9ETRQUKq3Sm+jcE0vQ==
9, 19 -- Content-Language: ro
9, 19 -- X-OriginalArrivalTime: 30 Jul 2009 10:18:15.0015 (UTC) FILETIME=[0CC0D770:01CA10FF]
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 30 Jul 2009 05:59:17 -0500
Subject: [Microscopy] Re: Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gary,
One advantage of posting to the list is the out of office notices. This
let's me check if the company server is up. Sometimes my in-box thinks its
the proverbially Maytag repairman.

Another possible use is to mine for companies which employes microscopist.
One could start a list of companies that might need another microscopist as
the economic world improves.

And finally, but not lastly, it generates interesting responses which make
me, (and others I hope ) smile.

Stay safe.........
Frank
Manager of Linked-In Microscopy Group

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
6, 22 -- From frank_karl-at-lincolnelectric.com Thu Jul 30 05:59:17 2009
6, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UAxGrg017183
6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 05:59:17 -0500
6, 22 -- In-Reply-To: {200907300511.n6U5BN3h012099-at-ns.microscopy.com}
6, 22 -- Subject: Re: [Microscopy] Out of office BS
6, 22 -- To: gary-at-gaugler.com, Microscopy-at-microscopy.com
6, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
6, 22 -- Message-ID: {OFA9EEDD36.C86D7A86-ON85257603.003B56C5-85257603.003C0FE4-at-lincolnelectric.com}
6, 22 -- Date: Thu, 30 Jul 2009 06:56:22 -0400
6, 22 -- From: Frank_Karl-at-lincolnelectric.com
6, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 07/30/2009 06:58:57 AM,
6, 22 -- CD-MIME complete at 07/30/2009 06:58:57 AM,
6, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 07/30/2009 06:58:57 AM,
6, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 22 -- 07, 2008) at 07/30/2009 06:58:57 AM,
6, 22 -- Serialize complete at 07/30/2009 06:58:57 AM
6, 22 -- MIME-Version: 1.0
6, 22 -- Content-Type: text/plain;
6, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: daveparm-at-yahoo.com
Date: Thu, 30 Jul 2009 09:56:04 -0500
Subject: [Microscopy] viaWWW: EDS Clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'd comment, but I'm currently out of the office attending M&M 2009.

Ira Bleiweiss
Smart Imaging Technologies
713.589.3216 direct
877.280.1100 ext.1004 Toll Free (US)

ira-at-simagis.us
www.smartimtech.com
Advanced Software Solutions for Science and Industry


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Thursday, July 30, 2009 12:11 AM
To: ira-at-simagis.us

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both daveparm-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: daveparm-at-yahoo.com
Name: David Parmiter

Organization: SAIC

Title-Subject: [Filtered] EDS Clarification

Question: Sorry to all if my previous message was a bit vague. We
have an older version of Genesis on an S3000 SEM, which works well
but doesn't have the resolution we need.

I'd like to know if anyone has used Genesis on, say, an 80-120kV TEM.
I'm just looking for some general information on whatever map size
and frames count you usually collect - i.e. How long the map takes to
acquire, how good you feel its resolution is, etc.

Please let me know if further clarification is needed.

Thanks again!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Jul 30 09:56:04 2009
9, 11 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UEu1Sa023061
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 09:56:02 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c69764ed9cfc-at-[172.16.0.101]}
9, 11 -- Date: Thu, 30 Jul 2009 10:55:57 -0400
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: daveparm-at-yahoo.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: EDS Clarification
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Adena.Rollins-at-sandisk.com
Date: Thu, 30 Jul 2009 09:56:36 -0500
Subject: [Microscopy] viaWWW:Manual Denton Desk II Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Adena.Rollins-at-sandisk.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Adena.Rollins-at-sandisk.com
Name: Adena Rollins

Organization: SanDisk

Title-Subject: [Filtered] RE: Denton Desk II Sputter Coater

Question: Does anyone have the manual for a Denton Desk II Sputter
Coater?? My power switch has went out and I need the schematics to
be able to work on it myself. It's going to take a while to get the
vendor approved for me to order one and I need it fixed ASAP!

Thank you all very much for ANY help with this matter!

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Jul 30 09:56:35 2009
7, 11 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UEuX0m023320
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 09:56:34 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c697650ea4cb-at-[172.16.0.101]}
7, 11 -- Date: Thu, 30 Jul 2009 10:56:31 -0400
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: Adena.Rollins-at-sandisk.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW:Manual Denton Desk II Sputter Coater
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Thu, 30 Jul 2009 11:15:29 -0500
Subject: [Microscopy] viaWWW:Manual Denton Desk II Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Adena,
I have the Denton Desk II sputter coater and the "Operating Instructions"
that came with it. Do you want the big, fold-out schematic? I would think
that the main power switch would be relatively straight forward to replace.
I have also found the people at Denton Vacuum to be very helpful and able to
supply replacement parts.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: Adena.Rollins-at-sandisk.com [mailto:Adena.Rollins-at-sandisk.com]
Sent: July 30, 2009 8:20 AM
To: maryflet-at-interchange.ubc.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Adena.Rollins-at-sandisk.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Adena.Rollins-at-sandisk.com
Name: Adena Rollins

Organization: SanDisk

Title-Subject: [Filtered] RE: Denton Desk II Sputter Coater

Question: Does anyone have the manual for a Denton Desk II Sputter
Coater?? My power switch has went out and I need the schematics to
be able to work on it myself. It's going to take a while to get the
vendor approved for me to order one and I need it fixed ASAP!

Thank you all very much for ANY help with this matter!

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Jul 30 09:56:35 2009
7, 11 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov
[130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n6UEuX0m023320
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 09:56:34
-0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c697650ea4cb-at-[172.16.0.101]}
7, 11 -- Date: Thu, 30 Jul 2009 10:56:31 -0400
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: Adena.Rollins-at-sandisk.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW:Manual Denton Desk II Sputter Coater
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
16, 33 -- From maryflet-at-interchange.ubc.ca Thu Jul 30 11:15:29 2009
16, 33 -- Received: from mr7.mail-relay.ubc.ca (mr7.mail-relay.ubc.ca [137.82.45.13])
16, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UGFTj2022797
16, 33 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 11:15:29 -0500
16, 33 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
16, 33 -- by mr7.mail-relay.ubc.ca (Postfix) with ESMTP id 3BDAC1C2A0
16, 33 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 09:15:28 -0700 (PDT)
16, 33 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
16, 33 -- by smtp.interchange.ubc.ca
16, 33 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
16, 33 -- with ESMTP id {0KNL00BKXRTRK2-at-smtp.interchange.ubc.ca} for
16, 33 -- microscopy-at-microscopy.com; Thu, 30 Jul 2009 09:15:28 -0700 (PDT)
16, 33 -- Date: Thu, 30 Jul 2009 09:15:24 -0700
16, 33 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
16, 33 -- Subject: RE: [Microscopy] viaWWW:Manual Denton Desk II Sputter Coater
16, 33 -- In-reply-to: {200907301520.n6UFK7du019515-at-ns.microscopy.com}
16, 33 -- To: Adena.Rollins-at-sandisk.com
16, 33 -- Cc: microscopy-at-microscopy.com
16, 33 -- Reply-to: maryflet-at-interchange.ubc.ca
16, 33 -- Message-id: {0KNL00BKYRTRK2-at-smtp.interchange.ubc.ca}
16, 33 -- Organization: Materials Eng.
16, 33 -- MIME-version: 1.0
16, 33 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
16, 33 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
16, 33 -- Content-type: text/plain; charset=us-ascii
16, 33 -- Content-transfer-encoding: 7bit
16, 33 -- Thread-index: AcoRKTq49Tq8uH5XQ3KeupK9CMog7gABvFdw
16, 33 -- X-UBC-Scanned: Sophos PureMessage 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.7.30.160048
16, 33 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
16, 33 -- X-PerlMx-Spam: Probability=8%, Report=
16, 33 -- BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __USER_AGENT_MS_GENERIC 0
16, 33 -- X-Spam-Level:
16, 33 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Thu, 30 Jul 2009 13:05:10 -0500
Subject: [Microscopy] Re: Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear, dear Gary-

You can be totally, completely, 100% sure, each and every year, to get Out
of Office responses during the week of the Microscopy & Microanalysis
conference, since so many of our colleagues are there.

The workaround for this is for *you* to unsubscribe for that week. That
will dramatically cut down on the traffic.

With much aloha,
Tina
(who is in the office and missed M&M this year)


} Why post anything to the list?
}
} I get 10-15 out of office crap.
}
} Ridiculous.
}
} Thanks...I'll get another 15 OOF msgs to this one.
}
} gary g.
}
}
} ==============================Original Headers==============================
} 6, 17 -- From gary-at-gaugler.com Thu Jul 30 00:06:22 2009
} 6, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
} 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6U56LHq032455
} 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 00:06:22 -0500
} 6, 17 -- Message-Id: {200907300506.n6U56LHq032455-at-ns.microscopy.com}
} 6, 17 -- Received: (qmail 16984 invoked from network); 29 Jul 2009 22:30:28 -0700
} 6, 17 -- Received: by simscan 1.1.0 ppid: 16979, pid: 16981, t: 0.1339s
} 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
} 6, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
} 6, 17 -- by smtp1 with SMTP; 29 Jul 2009 22:30:28 -0700
} 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 6, 17 -- Date: Wed, 29 Jul 2009 22:05:43 -0700
} 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
} 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
} 6, 17 -- Subject: Out of office BS
} 6, 17 -- Mime-Version: 1.0
} 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
8, 21 -- From tina-at-pbrc.hawaii.edu Thu Jul 30 13:05:09 2009
8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UI599f014626
8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 13:05:09 -0500
8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n6UI53vi017302
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
8, 21 -- Thu, 30 Jul 2009 08:05:04 -1000 (HST)
8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n6UI5256017298;
8, 21 -- Thu, 30 Jul 2009 08:05:03 -1000 (HST)
8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
8, 21 -- Date: Thu, 30 Jul 2009 08:05:02 -1000 (HST)
8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 21 -- X-Sender: tina-at-halia
8, 21 -- To: gary-at-gaugler.com
8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 21 -- Subject: Re: [Microscopy] Out of office BS
8, 21 -- In-Reply-To: {200907300507.n6U57AsQ001471-at-ns.microscopy.com}
8, 21 -- Message-ID: {Pine.GSO.4.21.0907300801450.17270-100000-at-halia}
8, 21 -- MIME-Version: 1.0
8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Thu, 30 Jul 2009 13:23:42 -0500
Subject: [Microscopy] Out of Office - what would happen if....?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Since the Out of Office debate has been running by my mail window (as it
does every year about this time, along with the ragweed pollen), I can't
stop thinking about what would happen if you sneaked (snuck?) onto
someone's computer who had the "Out of Office" thing set, and sent an
email to somebody else who also had an "Out of Office" thing set?
There's probably some bits somewhere that tell the software not to
continue bouncing the message, but wouldn't it be sweet justice to have
these people come back with 10 million little gifts in their mailbox?

Deviously yours,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
7, 18 -- From jehrman-at-mta.ca Thu Jul 30 13:23:42 2009
7, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.204])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UINfOr030391
7, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 13:23:42 -0500
7, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:51635)
7, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
7, 18 -- with esmtp id 1MWaJN-0006eO-VG (Exim 4.69) for Microscopy-at-microscopy.com
7, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 30 Jul 2009 15:25:06 -0300
7, 18 -- Message-ID: {4A71E3B4.3000801-at-mta.ca}
7, 18 -- Date: Thu, 30 Jul 2009 15:17:24 -0300
7, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
7, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
7, 18 -- MIME-Version: 1.0
7, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
7, 18 -- Subject: Out of Office - what would happen if....?
7, 18 -- X-Enigmail-Version: 0.96.0
7, 18 -- Content-Type: text/plain; charset=ISO-8859-1
7, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: lherault-at-bu.edu
Date: Thu, 30 Jul 2009 14:28:03 -0500
Subject: [Microscopy] Re: Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One solution to Out of Office messages, if you use Outlook, would be to
create a "rule" which sends messages with that phrase to the junk folder.

Ron L

-----Original Message-----
X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu]
Sent: Thursday, July 30, 2009 2:09 PM
To: lherault-at-bu.edu

Dear, dear Gary-

You can be totally, completely, 100% sure, each and every year, to get Out
of Office responses during the week of the Microscopy & Microanalysis
conference, since so many of our colleagues are there.

The workaround for this is for *you* to unsubscribe for that week. That
will dramatically cut down on the traffic.

With much aloha,
Tina
(who is in the office and missed M&M this year)


} Why post anything to the list?
}
} I get 10-15 out of office crap.
}
} Ridiculous.
}
} Thanks...I'll get another 15 OOF msgs to this one.
}
} gary g.
}
}
} ==============================Original
Headers==============================
} 6, 17 -- From gary-at-gaugler.com Thu Jul 30 00:06:22 2009
} 6, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
} 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n6U56LHq032455
} 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 00:06:22
-0500
} 6, 17 -- Message-Id: {200907300506.n6U56LHq032455-at-ns.microscopy.com}
} 6, 17 -- Received: (qmail 16984 invoked from network); 29 Jul 2009
22:30:28 -0700
} 6, 17 -- Received: by simscan 1.1.0 ppid: 16979, pid: 16981, t: 0.1339s
} 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
} 6, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
} 6, 17 -- by smtp1 with SMTP; 29 Jul 2009 22:30:28 -0700
} 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} 6, 17 -- Date: Wed, 29 Jul 2009 22:05:43 -0700
} 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
} 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
} 6, 17 -- Subject: Out of office BS
} 6, 17 -- Mime-Version: 1.0
} 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} ==============================End of -
Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *

* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*

****************************************************************************


==============================Original Headers==============================
8, 21 -- From tina-at-pbrc.hawaii.edu Thu Jul 30 13:05:09 2009
8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu
[128.171.22.7])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n6UI599f014626
8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 13:05:09
-0500
8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id
n6UI53vi017302
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NO);
8, 21 -- Thu, 30 Jul 2009 08:05:04 -1000 (HST)
8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu
(8.12.11/8.12.11/Submit) with ESMTP id n6UI5256017298;
8, 21 -- Thu, 30 Jul 2009 08:05:03 -1000 (HST)
8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process
doing -bs
8, 21 -- Date: Thu, 30 Jul 2009 08:05:02 -1000 (HST)
8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 21 -- X-Sender: tina-at-halia
8, 21 -- To: gary-at-gaugler.com
8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 21 -- Subject: Re: [Microscopy] Out of office BS
8, 21 -- In-Reply-To: {200907300507.n6U57AsQ001471-at-ns.microscopy.com}
8, 21 -- Message-ID: {Pine.GSO.4.21.0907300801450.17270-100000-at-halia}
8, 21 -- MIME-Version: 1.0
8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================


==============================Original Headers==============================
18, 22 -- From lherault-at-bu.edu Thu Jul 30 14:28:03 2009
18, 22 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62])
18, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UJS2QV016454
18, 22 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 14:28:02 -0500
18, 22 -- X-Envelope-From: lherault-at-bu.edu
18, 22 -- Received: from BUSDM801203 ([155.41.214.86])
18, 22 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n6UJRGeP021002
18, 22 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 15:27:16 -0400
18, 22 -- From: "Ron L'Herault" {lherault-at-bu.edu}
18, 22 -- To: {microscopy-at-microscopy.com}
18, 22 -- References: {200907301808.n6UI8bkA019406-at-ns.microscopy.com}
18, 22 -- Subject: RE: [Microscopy] Re: Out of office BS
18, 22 -- Date: Thu, 30 Jul 2009 15:27:16 -0400
18, 22 -- Message-ID: {006601ca114b$bf92aa80$56d6299b-at-ad.bu.edu}
18, 22 -- MIME-Version: 1.0
18, 22 -- Content-Type: text/plain;
18, 22 -- charset="us-ascii"
18, 22 -- Content-Transfer-Encoding: 7bit
18, 22 -- X-Mailer: Microsoft Office Outlook 11
18, 22 -- In-Reply-To: {200907301808.n6UI8bkA019406-at-ns.microscopy.com}
18, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
18, 22 -- Thread-Index: AcoRQPRvZFBkgd3GTf2AU8b7ymuedAACqGwQ
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Thu, 30 Jul 2009 14:28:49 -0500
Subject: [Microscopy] Fwd: Re: Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just consider this a byproduct of posting. Any time of year I post,
I get a bunch. The good thing, I now know how to say "I am out of
office" in German, French, Italian, Polish, Japanese...

These messages are easy to filter to a special little box or just to
Trash.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear, dear Gary-
}
} You can be totally, completely, 100% sure, each and every year, to
} get Out
} of Office responses during the week of the Microscopy & Microanalysis
} conference, since so many of our colleagues are there.
}
} The workaround for this is for *you* to unsubscribe for that week.
} That
} will dramatically cut down on the traffic.
}
} With much aloha,
} Tina
} (who is in the office and missed M&M this year)
}
}
} } Why post anything to the list?
} }
} } I get 10-15 out of office crap.
} }
} } Ridiculous.
} }
} } Thanks...I'll get another 15 OOF msgs to this one.
} }
} } gary g.
} }
} }
} } ==============================Original
} } Headers==============================
} } 6, 17 -- From gary-at-gaugler.com Thu Jul 30 00:06:22 2009
} } 6, 17 -- Received: from smtp1.mc.surewest.net
} } (qsmtp.mc.surewest.net [66.60.130.145])
} } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
} } id n6U56LHq032455
} } 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009
} } 00:06:22 -0500
} } 6, 17 -- Message-Id: {200907300506.n6U56LHq032455-at-ns.microscopy.com}
} } 6, 17 -- Received: (qmail 16984 invoked from network); 29 Jul 2009
} } 22:30:28 -0700
} } 6, 17 -- Received: by simscan 1.1.0 ppid: 16979, pid: 16981, t:
} } 0.1339s
} } 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
} } 6, 17 -- Received: from unknown (HELO thor.gaugler.com)
} } (66.60.171.211)
} } 6, 17 -- by smtp1 with SMTP; 29 Jul 2009 22:30:28 -0700
} } 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} } 6, 17 -- Date: Wed, 29 Jul 2009 22:05:43 -0700
} } 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
} } 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
} } 6, 17 -- Subject: Out of office BS
} } 6, 17 -- Mime-Version: 1.0
} } 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} } ==============================End of -
} } Headers==============================
} }
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho *
} tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808)
} 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
} ==============================Original
} Headers==============================
} 8, 21 -- From tina-at-pbrc.hawaii.edu Thu Jul 30 13:05:09 2009
} 8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu
} [128.171.22.7])
} 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n6UI599f014626
} 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 13:05:09
} -0500
} 8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
} 8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id
} n6UI53vi017302
} 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NO);
} 8, 21 -- Thu, 30 Jul 2009 08:05:04 -1000 (HST)
} 8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu
} (8.12.11/8.12.11/Submit) with ESMTP id n6UI5256017298;
} 8, 21 -- Thu, 30 Jul 2009 08:05:03 -1000 (HST)
} 8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned
} process doing -bs
} 8, 21 -- Date: Thu, 30 Jul 2009 08:05:02 -1000 (HST)
} 8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} 8, 21 -- X-Sender: tina-at-halia
} 8, 21 -- To: gary-at-gaugler.com
} 8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
} 8, 21 -- Subject: Re: [Microscopy] Out of office BS
} 8, 21 -- In-Reply-To: {200907300507.n6U57AsQ001471-at-ns.microscopy.com}
} 8, 21 -- Message-ID: {Pine.GSO.4.21.0907300801450.17270-100000-at-halia}
} 8, 21 -- MIME-Version: 1.0
} 8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
8, 23 -- From vladislav_speransky-at-nih.gov Thu Jul 30 14:28:49 2009
8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UJSnhK017476
8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 14:28:49 -0500
8, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id 112EA3BDADF;
8, 23 -- Thu, 30 Jul 2009 15:28:48 -0400 (EDT)
8, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
8, 23 -- by compute1.internal (MEProxy); Thu, 30 Jul 2009 15:28:48 -0400
8, 23 -- X-Sasl-enc: RnLrJ4ZlmxghnHc8TssQP0pK0X48Xetsgey/h/GIQRBJ 1248982127
8, 23 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id C78F6765;
8, 23 -- Thu, 30 Jul 2009 15:28:47 -0400 (EDT)
8, 23 -- Message-Id: {27940548-F5A2-4A4E-BE63-A06EDF292A13-at-nih.gov}
8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Subject: Fwd: [Microscopy] Re: Out of office BS
8, 23 -- Mime-Version: 1.0 (Apple Message framework v935.3)
8, 23 -- Date: Thu, 30 Jul 2009 15:28:46 -0400
8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2D379035-at-NIHMLBX02.nih.gov}
8, 23 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 30 Jul 2009 17:08:57 -0500
Subject: [Microscopy] Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That is a novel idea! I do have filters set up in
Eudora but they do not catch all of the OoO messages.
Before my ISP switched to Mailguard, they used Spam
Assassin. SA allowed spefic training with phrases,
Bayesen logic, etc. Mailguard just does white and black
list for addresses--no shot at phrases.

OoO happens often but you are right about the timing
with M&M. Good suggestion. The other option is to just
not post anything.

gary g.


At 11:07 AM 7/30/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Thu Jul 30 17:08:57 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6UM8v4d026212
9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 17:08:57 -0500
9, 20 -- Message-Id: {200907302208.n6UM8v4d026212-at-ns.microscopy.com}
9, 20 -- Received: (qmail 32730 invoked from network); 30 Jul 2009 14:56:41 -0700
9, 20 -- Received: by simscan 1.1.0 ppid: 32719, pid: 32721, t: 0.9599s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 30 Jul 2009 14:56:40 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Thu, 30 Jul 2009 15:08:21 -0700
9, 20 -- To: tina-at-pbrc.hawaii.edu
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] Re: Out of office BS
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- References: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 30 Jul 2009 17:52:12 -0500
Subject: [Microscopy] Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

:-) :-) :-) :-) :-) :-) :-) :-)

Or you could use Mailwasher and set a filter for "microscopy" plus "out of
office" but then you'd miss out on the "out of Office BS" because it would
get deleted. Ain't life a ...

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Thursday, July 30, 2009 6:12 PM
To: kenconverse-at-qualityimages.biz

That is a novel idea! I do have filters set up in
Eudora but they do not catch all of the OoO messages.
Before my ISP switched to Mailguard, they used Spam
Assassin. SA allowed spefic training with phrases,
Bayesen logic, etc. Mailguard just does white and black
list for addresses--no shot at phrases.

OoO happens often but you are right about the timing
with M&M. Good suggestion. The other option is to just
not post anything.

gary g.


At 11:07 AM 7/30/2009, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Thu Jul 30 17:08:57 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n6UM8v4d026212
9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 17:08:57
-0500
9, 20 -- Message-Id: {200907302208.n6UM8v4d026212-at-ns.microscopy.com}
9, 20 -- Received: (qmail 32730 invoked from network); 30 Jul 2009 14:56:41
-0700
9, 20 -- Received: by simscan 1.1.0 ppid: 32719, pid: 32721, t: 0.9599s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 30 Jul 2009 14:56:40 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Thu, 30 Jul 2009 15:08:21 -0700
9, 20 -- To: tina-at-pbrc.hawaii.edu
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] Re: Out of office BS
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- References: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================





==============================Original Headers==============================
23, 25 -- From kenconverse-at-qualityimages.biz Thu Jul 30 17:52:12 2009
23, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UMqCoG010489
23, 25 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 17:52:12 -0500
23, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
23, 25 -- with ESMTP
23, 25 -- id {20090730225211561.ZZLK24863-at-cdptpa-omta03.mail.rr.com} ;
23, 25 -- Thu, 30 Jul 2009 22:52:11 +0000
23, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
23, 25 -- To: {gary-at-gaugler.com} , "MSA Listserver" {microscopy-at-microscopy.com}
23, 25 -- Subject: RE: [Microscopy] Out of office BS
23, 25 -- Date: Thu, 30 Jul 2009 18:52:03 -0400
23, 25 -- Message-ID: {34E4639E390E48E1AD50F5179A868836-at-Ken}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain;
23, 25 -- charset="us-ascii"
23, 25 -- X-Priority: 3 (Normal)
23, 25 -- X-MSMail-Priority: Normal
23, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
23, 25 -- Importance: Normal
23, 25 -- Thread-Index: AcoRZxhF4A0QoAOZQ1Ch5vowyy0FyQAAF6KQ
23, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 25 -- In-Reply-To: {200907302211.n6UMBiib030260-at-ns.microscopy.com}
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6UMqCoG010489
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 30 Jul 2009 18:07:16 -0500
Subject: [Microscopy] Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You know, Gary, this is a real problem because now I have to go through all
of my automatically deleted OoO emails to find any REAL replies. Ain't life
a .

Ken Converse

owner


QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Thursday, July 30, 2009 6:12 PM
To: kenconverse-at-qualityimages.biz

That is a novel idea! I do have filters set up in
Eudora but they do not catch all of the OoO messages.
Before my ISP switched to Mailguard, they used Spam
Assassin. SA allowed spefic training with phrases,
Bayesen logic, etc. Mailguard just does white and black
list for addresses--no shot at phrases.

OoO happens often but you are right about the timing
with M&M. Good suggestion. The other option is to just
not post anything.

gary g.


At 11:07 AM 7/30/2009, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Thu Jul 30 17:08:57 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n6UM8v4d026212
9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 17:08:57
-0500
9, 20 -- Message-Id: {200907302208.n6UM8v4d026212-at-ns.microscopy.com}
9, 20 -- Received: (qmail 32730 invoked from network); 30 Jul 2009 14:56:41
-0700
9, 20 -- Received: by simscan 1.1.0 ppid: 32719, pid: 32721, t: 0.9599s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 30 Jul 2009 14:56:40 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Thu, 30 Jul 2009 15:08:21 -0700
9, 20 -- To: tina-at-pbrc.hawaii.edu
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] Re: Out of office BS
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- References: {200907301807.n6UI7CFe017180-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================





==============================Original Headers==============================
24, 25 -- From kenconverse-at-qualityimages.biz Thu Jul 30 18:07:16 2009
24, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UN7GpC025516
24, 25 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 18:07:16 -0500
24, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
24, 25 -- with ESMTP
24, 25 -- id {20090730230715779.JTM24863-at-cdptpa-omta03.mail.rr.com} ;
24, 25 -- Thu, 30 Jul 2009 23:07:15 +0000
24, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
24, 25 -- To: {gary-at-gaugler.com} , "MSA Listserver" {microscopy-at-microscopy.com}
24, 25 -- Subject: RE: [Microscopy] Out of office BS
24, 25 -- Date: Thu, 30 Jul 2009 19:07:07 -0400
24, 25 -- Message-ID: {AAA3AAAB25DE403B85AD39F3C7F5E0C8-at-Ken}
24, 25 -- MIME-Version: 1.0
24, 25 -- Content-Type: text/plain;
24, 25 -- charset="us-ascii"
24, 25 -- X-Priority: 3 (Normal)
24, 25 -- X-MSMail-Priority: Normal
24, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
24, 25 -- Importance: Normal
24, 25 -- Thread-Index: AcoRZxhF4A0QoAOZQ1Ch5vowyy0FyQAAw/wg
24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 25 -- In-Reply-To: {200907302211.n6UMBiib030260-at-ns.microscopy.com}
24, 25 -- Content-Transfer-Encoding: 8bit
24, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6UN7GpC025516
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Thu, 30 Jul 2009 18:40:50 -0500
Subject: [Microscopy] Out of office BS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Personally, I used to find the out of office replies annoying until I
had the realization of how many languages I was getting them in. That
gave me pause to realize just how large this community is.

And then again, there is that momentary rush when I look at my email
client and see "48 new messages" and think that for one moment I am
really popular or needed. It's similar to the euphoria I get when I
find a five dollar bill in a pocket that I had forgotten about. ;)

That, and, well, I wish I had an office to be out of from time to time. :)

--Justin A. Kraft

On Thu, Jul 30, 2009 at 7:11 PM, {kenconverse-at-qualityimages.biz} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} You know, Gary, this is a real problem because now I have to go through all
} of my automatically deleted OoO emails to find any REAL replies.  Ain't life
} a .
}
} Ken Converse
}
} owner
}
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME  04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
} Sent: Thursday, July 30, 2009 6:12 PM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] Out of office BS
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} That is a novel idea!  I do have filters set up in
} Eudora but they do not catch all of the OoO messages.
} Before my ISP switched to Mailguard, they used Spam
} Assassin.  SA allowed spefic training with phrases,
} Bayesen logic, etc.  Mailguard just does white and black
} list for addresses--no shot at phrases.
}
} OoO happens often but you are right about the timing
} with M&M.  Good suggestion.  The other option is to just
} not post anything.
}
} gary g.
}
}
} At 11:07 AM 7/30/2009, you wrote:
}
}
}
} } ---------------------------------------------------------------------------
} -
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver



--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar


==============================Original Headers==============================
9, 35 -- From kraftpiano-at-gmail.com Thu Jul 30 18:40:50 2009
9, 35 -- Received: from mail-ew0-f211.google.com (mail-ew0-f211.google.com [209.85.219.211])
9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6UNen30008391
9, 35 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 18:40:49 -0500
9, 35 -- Received: by ewy7 with SMTP id 7so724003ewy.18
9, 35 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2009 16:40:49 -0700 (PDT)
9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
9, 35 -- d=gmail.com; s=gamma;
9, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
9, 35 -- :date:message-id:subject:from:to:content-type
9, 35 -- :content-transfer-encoding;
9, 35 -- bh=1O5xjtjrjkCZ3V8uUItlFlT6cVZJSz3bRXas4aC+iRs=;
9, 35 -- b=fmGUMs0LveQlh8LhEmal4LUFeYA92sKkvFpV6uOJF2gPUvJIb5Az4og2BQD2JyfSJD
9, 35 -- Ptyi4kh4nFllogntNYws902h/wUa+LIswqaWK0/grv3kg9pGKguJ0ImYYgaRAbPm6435
9, 35 -- wHHUTX4oVftudk1M04+Dnn7KxfCnhIT6H1ykE=
9, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
9, 35 -- d=gmail.com; s=gamma;
9, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
9, 35 -- :content-type:content-transfer-encoding;
9, 35 -- b=b+u4S5UMVmynCrIoUaeRGivVWL/VFtz/8DaO2MSKEQeSI02z8Ki/IWuIsDGSFtVqah
9, 35 -- VXQlCBeGDqnAVC5wOTqLLW9+pynmQd6HOZhGuAiEpa6IIuAt3c1eMJlLaFbjUerNJ8a5
9, 35 -- 54WA5t48emWxCN2ZSDzPmEGHhyZF9psKxinM0=
9, 35 -- MIME-Version: 1.0
9, 35 -- Received: by 10.216.53.196 with SMTP id g46mr389729wec.63.1248997248590; Thu,
9, 35 -- 30 Jul 2009 16:40:48 -0700 (PDT)
9, 35 -- In-Reply-To: {200907302311.n6UNBBT5002022-at-ns.microscopy.com}
9, 35 -- References: {200907302311.n6UNBBT5002022-at-ns.microscopy.com}
9, 35 -- Date: Thu, 30 Jul 2009 19:40:48 -0400
9, 35 -- Message-ID: {25e2b0d20907301640x653f297w99fb08cf488f327e-at-mail.gmail.com}
9, 35 -- Subject: Re: [Microscopy] RE: Out of office BS
9, 35 -- From: Justin Kraft {kraftpiano-at-gmail.com}
9, 35 -- To: kenconverse-at-qualityimages.biz, microscopy-at-microscopy.com
9, 35 -- Content-Type: text/plain; charset=ISO-8859-1
9, 35 -- Content-Transfer-Encoding: 8bit
9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6UNen30008391
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Fri, 31 Jul 2009 00:15:39 -0500
Subject: [Microscopy] LM: Microscope lubrication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What lubricant is suitable for a sticky dovetail slide mechanism?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
3, 24 -- From donc-at-asmicro.com Fri Jul 31 00:15:39 2009
3, 24 -- Received: from smtp104.sbc.mail.mud.yahoo.com (smtp104.sbc.mail.mud.yahoo.com [68.142.198.203])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6V5FcKa007870
3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 00:15:39 -0500
3, 24 -- Received: (qmail 38409 invoked from network); 31 Jul 2009 05:15:37 -0000
3, 24 -- Received: from unknown (HELO asm15) (donc-at-98.226.78.153 with login)
3, 24 -- by smtp104.sbc.mail.mud.yahoo.com with SMTP; 31 Jul 2009 05:15:37 -0000
3, 24 -- X-YMail-OSG: KdDozjIVM1l20IPLMtRKZge9UfAhkr0zohrWkjJursZM7WVMeva8NX07hyuOPrVV1TjJ0Hwjd6SoOayAYp6v_qFncxwzlH5q1tyRtNHnpLHLl94FbuMvBvco8I2qjL6MkuprMwgzijckxTDAqMEc2vcxKhr46VU0MNyxHU5gak4IbfaquOZ0p.w5VYawEzkOFZ3h4hx1YCXbvpwXtdcnTjr48QDE2BsHC0b6m_ATBmABAHDS8n.8x_fP9NrLdZPxzX0KKneD7FKlSG097vPyXMbDUvT2HkhmP41LYPJv1Q3xmaz8vZb6VLvEGuZ13Cwqo6Du5cg3aGUeD8VoJ01CRmaa_dH1EziTiQfn
3, 24 -- X-Yahoo-Newman-Property: ymail-3
3, 24 -- Message-ID: {AAE54FA878BA48709532552D1E939AC9-at-asm15}
3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
3, 24 -- Subject: LM: Microscope lubrication
3, 24 -- Date: Fri, 31 Jul 2009 01:15:28 -0400
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- format=flowed;
3, 24 -- charset="iso-8859-1";
3, 24 -- reply-type=original
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- X-Priority: 3
3, 24 -- X-MSMail-Priority: Normal
3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: msf-at-forensica.com
Date: Fri, 31 Jul 2009 06:07:38 -0500
Subject: [Microscopy] viaWWW: Old EDX equipment for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both msf-at-forensica.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: msf-at-forensica.com
Name: Mark Floyd

Organization: Forensic Analytical Laboratories

Title-Subject: [Filtered] Old EDX equipment for donation

Question: Available for donation:
1. Kevex Delta Class EDX analyzer, keyboard, manuals & software. Was
a working system until hard drive failed.
2. EDAX 9800 analyzer, keyboard, manuals & software. Was a working
system until hard drive failed.
No detectors or monitors available. Hoping to help someone revive an
older EDX system. These items are about 20 years old.
Prefer local pickup (Hayward, CA 94545), but will ship part or all at
recipient's expense to US addresses only.
For photos or more details, email msf-at-forensica.com offline.
Remainders will be picked up by electronic recycler no later than Aug 7, 2009
Mark Floyd
Forensic Analytical Laboratories


Login Host: 206.169.98.180
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Jul 31 06:07:38 2009
7, 11 -- Received: from [172.16.0.101] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6VB7bTa008935
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 06:07:38 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c69880dc4f89-at-[172.16.0.101]}
7, 11 -- Date: Fri, 31 Jul 2009 07:07:36 -0400
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: msf-at-forensica.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Old EDX equipment for donation
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Fri, 31 Jul 2009 16:31:08 -0500
Subject: [Microscopy] LM: Microscope lubrication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

First clean it. Start with some WD-40 and some cotton and get the old sticky grease back off.

Once it is all clean apply a light coating of thin grease or a thin layer of heavy oil.



-----Original Message-----
X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com]
Sent: Fri 7/31/2009 1:24 AM
To: Williams, Geoffrey

What lubricant is suitable for a sticky dovetail slide mechanism?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================


==============================Original Headers==============================
3, 24 -- From donc-at-asmicro.com Fri Jul 31 00:15:39 2009
3, 24 -- Received: from smtp104.sbc.mail.mud.yahoo.com (smtp104.sbc.mail.mud.yahoo.com [68.142.198.203])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n6V5FcKa007870
3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 00:15:39 -0500
3, 24 -- Received: (qmail 38409 invoked from network); 31 Jul 2009 05:15:37 -0000
3, 24 -- Received: from unknown (HELO asm15) (donc-at-98.226.78.153 with login)
3, 24 -- by smtp104.sbc.mail.mud.yahoo.com with SMTP; 31 Jul 2009 05:15:37 -0000
3, 24 -- X-YMail-OSG: KdDozjIVM1l20IPLMtRKZge9UfAhkr0zohrWkjJursZM7WVMeva8NX07hyuOPrVV1TjJ0Hwjd6SoOayAYp6v_qFncxwzlH5q1tyRtNHnpLHLl94FbuMvBvco8I2qjL6MkuprMwgzijckxTDAqMEc2vcxKhr46VU0MNyxHU5gak4IbfaquOZ0p.w5VYawEzkOFZ3h4hx1YCXbvpwXtdcnTjr48QDE2BsHC0b6m_ATBmABAHDS8n.8x_fP9NrLdZPxzX0KKneD7FKlSG097vPyXMbDUvT2HkhmP41LYPJv1Q3xmaz8vZb6VLvEGuZ13Cwqo6Du5cg3aGUeD8VoJ01CRmaa_dH1EziTiQfn
3, 24 -- X-Yahoo-Newman-Property: ymail-3
3, 24 -- Message-ID: {AAE54FA878BA48709532552D1E939AC9-at-asm15}
3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com}
3, 24 -- Subject: LM: Microscope lubrication
3, 24 -- Date: Fri, 31 Jul 2009 01:15:28 -0400
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- format=flowed;
3, 24 -- charset="iso-8859-1";
3, 24 -- reply-type=original
3, 24 -- Content-Transfer-Encoding: 7bit
3, 24 -- X-Priority: 3
3, 24 -- X-MSMail-Priority: Normal
3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512
3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================



==============================Original Headers==============================
13, 29 -- From Geoffrey_Williams-at-brown.edu Fri Jul 31 16:30:58 2009
13, 29 -- Received: from honeydew.services.brown.edu (honeydew.services.brown.edu [128.148.106.194])
13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6VLUwQM015576
13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 16:30:58 -0500
13, 29 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53])
13, 29 -- by honeydew.services.brown.edu (8.14.3/8.14.3) with ESMTP id n6VLUvrw026941
13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 17:30:57 -0400
13, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959);
13, 29 -- Fri, 31 Jul 2009 17:30:57 -0400
13, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 29 -- Content-class: urn:content-classes:message
13, 29 -- MIME-Version: 1.0
13, 29 -- Content-Type: text/plain;
13, 29 -- charset="iso-8859-1"
13, 29 -- Subject: RE: [Microscopy] LM: Microscope lubrication
13, 29 -- Date: Fri, 31 Jul 2009 17:26:05 -0400
13, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F032FD40F-at-MAIL1.AD.Brown.Edu}
13, 29 -- X-MS-Has-Attach:
13, 29 -- X-MS-TNEF-Correlator:
13, 29 -- Thread-Topic: [Microscopy] LM: Microscope lubrication
13, 29 -- Thread-Index: AcoRnyBM7KXe2lt5Q66bbeTLNZeNPAAhmKWn
13, 29 -- References: {200907310524.n6V5O5mo021729-at-ns.microscopy.com}
13, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
13, 29 -- To: {donc-at-asmicro.com} , {microscopy-at-microscopy.com}
13, 29 -- X-OriginalArrivalTime: 31 Jul 2009 21:30:57.0327 (UTC) FILETIME=[30FAA3F0:01CA1226]
13, 29 -- X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 definitions=5694 signatures=542516
13, 29 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0907200000 definitions=main-0907310139
13, 29 -- Content-Transfer-Encoding: 8bit
13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6VLUwQM015576
==============================End of - Headers==============================




From: charlotte.ownby-at-okstate.edu
Date: Fri, 31 Jul 2009 16:36:47 -0500
Subject: [Microscopy] Technician Position in OSU Microscopy Lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Research Lab Technician
OSU Microscopy Lab


Summary of Duties and Responsibilities:

Operate, prepare samples and assist researchers with the operation of the Transmission Electron Microscope, Scanning Electron Microscope, Atomic Force Microscope and Laser Scanning Confocal Microscope. Ensure that researchers get suitable computer generated images and other pertinent data from the microscopes, and assist researchers with sample preparation for electron microscopy.

Assist Lab Manager in training researchers, lab technicians and students in the use of microscopes and other equipment in the Lab. Advance skills by learning new technologies relevant to the mission of the laboratory. Maintain laboratory equipment and ensure that all equipment is in good working order.

Assist the laboratory manager with keeping the laboratory stocked with necessary supplies, maintaining the chemical inventory, keeping the laboratory clean and orderly, and performing other duties as deemed necessary.

Qualifications/Requirements:

Bachelor’s degree required.
Good organization and excellent communication, and computer skills.
Good to excellent fine motor skills.
One to two years experience in microscopy is preferred.
Must sometimes perform work in a fume hood.
Must wear protective gloves and eyewear when handling some chemicals.
Ability to lift 35 pounds.

For full position description, please go to jobs.okstate.edu and refer to posting number 05568.

Complete application at OSU Human Resources, 106 Whitehurst or apply on-line at jobs.okstate.edu.

To receive full consideration, applications must be received no later than August 14, 2009.



“Oklahoma State University is an Affirmative Action/Equal Employment Opportunity employer committed to multicultural diversity. Oklahoma State University employs only U.S. citizens and lawfully authorized non-U.S. citizens. OSU participates in E-Verify to verify employment eligibility of all employees pursuant to the Oklahoma Taxpayer and Citizen Protection Act.”





Dr. Charlotte L. Ownby
Professor Emeritus
Director, OSU Microscopy Laboratory
Chair, OSU IACUC


OSU Microscopy Laboratory
1110 S. Innovation Way
Stillwater, OK 74074
Phone: 405-744-8087

==============================Original Headers==============================
12, 23 -- From charlotte.ownby-at-okstate.edu Fri Jul 31 16:36:46 2009
12, 23 -- Received: from smtp.ad.okstate.edu (smtp.ad.okstate.edu [139.78.133.2])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n6VLakWj022795
12, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jul 2009 16:36:46 -0500
12, 23 -- Received: from STWEXE2.ad.okstate.edu ([139.78.102.98]) by smtp.ad.okstate.edu
12, 23 -- ([139.78.133.2]) with mapi; Fri, 31 Jul 2009 16:36:45 -0500
12, 23 -- From: "Ownby, Charlotte" {charlotte.ownby-at-okstate.edu}
12, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
12, 23 -- CC: "Pogue, Jason R" {jason.pogue-at-okstate.edu}
12, 23 -- Date: Fri, 31 Jul 2009 16:36:45 -0500
12, 23 -- Subject: Technician Position in OSU Microscopy Lab
12, 23 -- Thread-Topic: Technician Position in OSU Microscopy Lab
12, 23 -- Thread-Index: AQHKEicAKie/ZcDZnki1hNXw8Hag2A==
12, 23 -- Message-ID: {A8863653D82073448887B2B7FA3380BB2BCA103815-at-STWEXE2.ad.okstate.edu}
12, 23 -- Accept-Language: en-US
12, 23 -- Content-Language: en-US
12, 23 -- X-MS-Has-Attach:
12, 23 -- X-MS-TNEF-Correlator:
12, 23 -- acceptlanguage: en-US
12, 23 -- Content-Type: text/plain; charset="Windows-1252"
12, 23 -- MIME-Version: 1.0
12, 23 -- Content-Transfer-Encoding: 8bit
12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n6VLakWj022795
==============================End of - Headers==============================




From: stegridl-at-aol.com
Date: Sat, 1 Aug 2009 16:33:59 -0500
Subject: [Microscopy] viaWWW: Leica m420 parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both stegridl-at-aol.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: stegridl-at-aol.com
Name: steve g

Organization: self

Title-Subject: [Filtered] Leica m420 parts

Question: I am looking for parts to a Leica M420 (or MZ
series)microscope including a stand with coarse and fine focus
control, carrier, and filter holder.

Login Host: 205.188.116.7
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Sat Aug 1 16:33:59 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n71LXwK5020430
6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 1 Aug 2009 16:33:59 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c69a6538d0cb-at-[206.69.208.22]}
6, 11 -- Date: Sat, 1 Aug 2009 16:33:57 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: stegridl-at-aol.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Leica m420 parts
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Sat, 1 Aug 2009 17:02:10 -0500
Subject: [Microscopy] RE: Lube for microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suggest trying one of the Fomblin or Krytox vacuum pump
oils. These perfluorinated compounds are excellent l.ubricants and
are so chemically inert that they are likely to ever become thick and
gummy.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
1, 15 -- From bigelow-at-umich.edu Sat Aug 1 17:02:10 2009
1, 15 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160])
1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n71M2AGW002732
1, 15 -- for {microscopy-at-microscopy.com} ; Sat, 1 Aug 2009 17:02:10 -0500
1, 15 -- Received: FROM [76.226.139.227] (adsl-76-226-139-227.dsl.sfldmi.sbcglobal.net [76.226.139.227])
1, 15 -- By skycaptain.mr.itd.umich.edu ID 4A74BB61.C62D7.26336 ;
1, 15 -- Authuser bigelow;
1, 15 -- 1 Aug 2009 18:02:09 EDT
1, 15 -- Mime-Version: 1.0
1, 15 -- Message-Id: {p06240800c69a6a75d5bb-at-[99.131.44.62]}
1, 15 -- Date: Sat, 1 Aug 2009 18:01:49 -0400
1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 15 -- Subject: [Microscopy]RE: Lube for microscope
1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Tue, 4 Aug 2009 15:02:43 -0500
Subject: [Microscopy] Ocean Optics Spectrometer recommendation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,
I'd like to upgrade my ancient Oxford panchromatic CL system to do
spectroscopy. It seems that one of the Ocean Optics spectrometers
would fit the bill. Has anyone on the list performed such an
modification/upgrade on their panchromatic system?

I was thinking of connecting the fiber optic to the unit just before
the PMT in place of the RGB filter plate. That way I could easily
switch from panchromatic to spectroscopy mode and back again in a few
seconds. I will have to rig some kind of an adapter to get the light
cone into a fiber optics but I think a small parabolic mirror or
prism might work. Or is there an off-the-shelf solution that anyone
knows about? The size of such an adapter would be pretty limited
because the filter plate slot is only 0.312" thick x 1.41" high (or
7.9mm x 35.8 mm).

I would like to cover the visible light range but maybe an extended
capability to the UV might be possible using a quartz fiber optic
cable? Which model spectrometer would be most suitable for microscopy?

Any suggestions would be welcome. Thanks.
john

John Donovan
Director- Microanalytical Facility
CAMCOR-UofO
541-346-4632
donovan-at-uoregon.edu
www.camcor.uoregon.edu
www.epmalab.uoregon.edu


==============================Original Headers==============================
6, 20 -- From donovan-at-uoregon.edu Tue Aug 4 15:02:43 2009
6, 20 -- Received: from smtp.uoregon.edu (mserv3.uoregon.edu [128.223.142.101])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n74K2giu016161
6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Aug 2009 15:02:43 -0500
6, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46])
6, 20 -- (authenticated bits=0)
6, 20 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n74K2eD4026492
6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
6, 20 -- Tue, 4 Aug 2009 13:02:41 -0700
6, 20 -- Message-Id: {200908042002.n74K2eD4026492-at-smtp.uoregon.edu}
6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
6, 20 -- Date: Tue, 04 Aug 2009 13:02:36 -0700
6, 20 -- To: microscopy-at-microscopy.com
6, 20 -- From: John Donovan {donovan-at-uoregon.edu}
6, 20 -- Subject: Ocean Optics Spectrometer recommendation
6, 20 -- Mime-Version: 1.0
6, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
6, 20 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.8161:2.4.5,1.2.40,4.0.166 definitions=2009-08-04_09:2009-07-24,2009-08-04,2009-08-04 signatures=0
6, 20 -- X-Virus-Scanned: ClamAV 0.94.2/9653/Tue Aug 4 11:35:53 2009 on mserv3
6, 20 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Thu, 6 Aug 2009 12:14:20 -0500
Subject: [Microscopy] Support for core units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everybody,

I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December.

We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors.

Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture?

Please let me know your experiences.

Thanks,

Paula :-}


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.




==============================Original Headers==============================
13, 21 -- From vapatpxs-at-yahoo.com Thu Aug 6 12:14:19 2009
13, 21 -- Received: from web46104.mail.sp1.yahoo.com (web46104.mail.sp1.yahoo.com [68.180.199.121])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n76HEJW2002954
13, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 12:14:19 -0500
13, 21 -- Received: (qmail 22284 invoked by uid 60001); 6 Aug 2009 17:14:19 -0000
13, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1249578859; bh=0spHjN8fjWVOxrej0aZzLx11kJZuuL17bfRBBWtuI5M=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=yzRN5uAA15f2SbhXc0Z5xeFOYNqeol8+iNxld3ET3PUY7dwp0bYPx+v9GyINX+JtA7bYgOqTELIXJFZhyQ2ztXkJiS5MElh0jtUUseJggNuTukMjRUR0yLf0+y4sG3dJKe0xFOP2I/FVtcXJqsCVUEIsO7sDGGUdp/hcr4DaQGU=
13, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 21 -- s=s1024; d=yahoo.com;
13, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 21 -- b=bNC8PmaoJBb+G+hbT4L/Z2scVN6HqbSOuTp4mGI121M2CfmD80AOm/iHeGG3rjFeTVcoPhgDRtSwv2DAJt1na2O6VypI9U5vHIq2h7EEb4pKKPUkj18ht3rcUaZTxPCjyRqC+SUFC3AiUTITPfRiAt1PW+G/I9dl3Yxd5+5qCXQ=;
13, 21 -- Message-ID: {73199.21406.qm-at-web46104.mail.sp1.yahoo.com}
13, 21 -- X-YMail-OSG: jfEhGqsVM1kKXLFSEbAOsUjPcVsR_1Pl9c6zwqicFFbQz_UQ.RvS5x8BGqibxdbzUEbH00LvMYB6otZCl0yb00QzpHPfe6AMTS4WS9.0xPSX9aYWtvFlkNTGIX1kUY5waL3YBqI3vVtivdkGpaWTJ8GwjELVA8D94bK3rn6ljhDIIPOF3UzRnj9bkrGrmXsy8UWRqfvDigJrWHsmIB3r7AXPMjKOb9LXLG6IN5TCFWAWYVQpEPAWwCfmw6e9xAGvSZEsXMUlvH6s.I8_y6F91v_0s86H4oJT6okrww--
13, 21 -- Received: from [132.239.85.200] by web46104.mail.sp1.yahoo.com via HTTP; Thu, 06 Aug 2009 10:14:19 PDT
13, 21 -- X-Mailer: YahooMailClassic/6.1.2 YahooMailWebService/0.7.289.15
13, 21 -- Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT)
13, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 21 -- Subject: Support for core units
13, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
13, 21 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
13, 21 -- MIME-Version: 1.0
13, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: ZZhang-at-uwyo.edu
Date: Thu, 6 Aug 2009 13:36:32 -0500
Subject: [Microscopy] Support for core units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Paula:

The following is a forwarded email from Phil Oshel, who did a nation-wide survey in 2002 on the usage and support of core facilities. Hope this would help you (and possibly your CEO) get an some idea.

My own experience is that it is almost impossible to support a core facility solely by user fees. Someone told me that it is as easy as traveling to the moon.

Whether it is possible to collect $25K of recharge mainly depends on your user base - how many users and how much time your service is being used. You don't want to charge users too much (more than $50 per hour on average). It will chase your users away. With that said, you need at least 500 hours of usage between now to December in order to collect your $25K, without bankrupting your users.

I've been asked recently by my administration to "increase the user base, or recruit more users", so to cover the service contracts solely by user recharge. We are in a relatively small university, with limited faculty number and limited funding. Any advice on how and where I could dig out more users? Anyone out there want to become my user? I have a TEM, a Tabletop SEM, a confocal, two epi-fluorescence, and in the process of getting a flow cytometer.....


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038




===============BEGINNING OF FORWARDED EMAIL==========================
-----Original Message-----
X-from: Philip Oshel [mailto:peoshel-at-facstaff.wisc.edu]
Sent: Monday, July 22, 2002 2:45 PM
To: Microscopy-at-sparc5.microscopy.com


It spite of my best efforts to format the survey with Geneva
fixed-width font in ascii, it still came across to some folks (many?)
all disarranged. So Here is the survey reformatted by J Quinn at SUNY
(Stony Brook, I believe).
Phil
**************************************************

2. Nature of institution
COUNT %
Academic 50 89
Other 4 7
Private research 2 4
Commercial 1 2

56 Total Respondents
57 Total Responses

**************************************************

3. Type of facility
COUNT %
Institution core 32 58
Departmental 19 35
Other 5 9
Satellite 1 2

55 Total Respondents
57 Total Responses

**************************************************

4. Predominate work
COUNT %
Biological 53 87
Materials 27 44

61 Total Respondents
80 Total Responses

**************************************************

5. User Base
COUNT %
Multi - user 56 93
Service 47 78

60 Total Respondents
103 Total Responses

**************************************************

6. Type of microscopes
COUNT %
TEM 48 80
SEM 36 60
Optical 18 30
Confocal 18 30
Other 18 30
Other Optical 16 27
FESEM 12 20
ESEM or LV 12 20
Other Confocal 5 8
Scanning Probe 5 8
FETEM 3 5
Other Scanning Probe 3 5

60 Total Respondents
194 Total Responses

**************************************************

7. Salaries
COUNT %
100% Your Institution 31 50
80% Your Institution 6 10
50% Your Institution 5 8
100% User fees 4 6
20% Grants 4 6
100% Grants 4 6
10% Your Institution 3 5
20% User fees 3 5
25% User fees 3 5
30% User fees 3 5
50% User fees 3 5
25% Grants 3 5
70% Your Institution 2 3
75% Your Institution 2 3
10% User fees 2 3
10% Grants 2 3
50% Grants 2 3
Other 2 3
20% Your Institution 1 2
30% Your Institution 1 2
60% Your Institution 1 2
90% Your Institution 1 2
Other 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
Other User fee percentage 1 2
30% Grants 1 2
40% Grants 1 2
70% Grants 1 2
75% Grants 1 2
Other Grants 1 2

62 Total Respondents
98 Total Responses

**************************************************

8. Instrument maintenance
COUNT %
100% User fees 19 31
100% Your Institution 18 30
50% Your Institution 6 9
50% Grants 4 6
10% Your Institution 3 5
90% Your Institution 3 5
50% User fees 3 5
60% User fees 3 5
20% Your Institution 2 3
40% Your Institution 2 3
Other 2 3
10% User fees 2 3
80% User fees 2 3
90% User fees 2 3
10% Grants 2 3
20% Grants 2 3
100% Grants 2 3
25% Your Institution 1 2
30% Your Institution 1 2
25% User fees 1 2
30% User fees 1 2
60% Grants 1 2
75% Grants 1 2
Other 1 2
61 Total Respondents
84 Total Responses

**************************************************

9. Replacement of existing equipment
COUNT %
100% Grants 16 30
100% Your Institution 11 20
50% Your Institution 6 11
50% Grants 5 9
60% Grants 5 9
20% Your Institution 4 7
30% Your Institution 4 7
10% User fees 4 7
100% User fees 4 7
25% Your Institution 3 5
40% Your Institution 3 5
20% User fees 3 5
50% User fees 3 5
10% Your Institution 2 4
70% Grants 2 4
75% Grants 2 4
90% Grants 2 4
40% User fees 1 2
75% User fees 1 2
80% User fees 1 2
90% User fees 1 2
10% Grants 1 2
40% Grants 1 2
80% Grants 1 2
Other 1 2

56 Total Respondents
87 Total Responses

**************************************************

10. Purchasing of new equipment
COUNT %
100% Grants 21 37
100% Your Institution 9 16
50% Your Institution 6 11
50% Grants 6 11
20% Your Institution 5 9
30% Your Institution 5 9
70% Grants 4 7
20% User fees 3 5
60% Grants 3 5
80% Grants 3 5
10% Your Institution 2 4
25% Your Institution 2 4
40% Your Institution 2 4
75% Your Institution 2 4
10% User fees 2 4
30% User fees 2 4
100% User fees 2 4
10% Grants 2 4
90% Grants 2 4
60% Your Institution 1 2
70% Your Institution 1 2
40% User fees 1 2
25% Grants 1 2
40% Grants 1 2
75% Grants 1 2

57 Total Respondents
89 Total Responses

**************************************************

11. Supplies
COUNT %
100% User fees 28 46
100% Your Institution 9 15
50% Grants 5 8
80% Your Institution 4 7
10% Your Institution 3 5
50% Your Institution 3 5
50% User fees 3 5
90% User fees 3 5
10% Grants 3 5
90% Your Institution 2 3
10% User fees 2 3
30% User fees 2 3
20% Grants 2 3
100% Grants 2 3
20% Your Institution 1 2
25% Your Institution 1 2
30% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
20% User fees 1 2
25% User fees 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
40% Grants 1 2
70% Grants 1 2
90% Grants 1 2
Other 1 2

61 Total Respondents
85 Total Responses

**************************************************

12. Operating expenses
COUNT %
100% User fees 19 40
100% Your Institution 16 33
50% Your Institution 6 12
50% User fees 6 12
100% Grants 3 6
20% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
80% Your Institution 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
20% Grants 1 2
Other 1 2

48 Total Respondents
59 Total Responses

**************************************************
============END OF FORWARDED EMAIL================

-----Original Message-----
X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com]
Sent: Thursday, August 06, 2009 11:19 AM
To: Z.J. Zhang


Hello Everybody,

I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December.

We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors.

Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture?

Please let me know your experiences.

Thanks,

Paula :-}


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.




==============================Original Headers==============================
13, 21 -- From vapatpxs-at-yahoo.com Thu Aug 6 12:14:19 2009
13, 21 -- Received: from web46104.mail.sp1.yahoo.com (web46104.mail.sp1.yahoo.com [68.180.199.121])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n76HEJW2002954
13, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 12:14:19 -0500
13, 21 -- Received: (qmail 22284 invoked by uid 60001); 6 Aug 2009 17:14:19 -0000
13, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1249578859; bh=0spHjN8fjWVOxrej0aZzLx11kJZuuL17bfRBBWtuI5M=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=yzRN5uAA15f2SbhXc0Z5xeFOYNqeol8+iNxld3ET3PUY7dwp0bYPx+v9GyINX+JtA7bYgOqTELIXJFZhyQ2ztXkJiS5MElh0jtUUseJggNuTukMjRUR0yLf0+y4sG3dJKe0xFOP2I/FVtcXJqsCVUEIsO7sDGGUdp/hcr4DaQGU=
13, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 21 -- s=s1024; d=yahoo.com;
13, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 21 -- b=bNC8PmaoJBb+G+hbT4L/Z2scVN6HqbSOuTp4mGI121M2CfmD80AOm/iHeGG3rjFeTVcoPhgDRtSwv2DAJt1na2O6VypI9U5vHIq2h7EEb4pKKPUkj18ht3rcUaZTxPCjyRqC+SUFC3AiUTITPfRiAt1PW+G/I9dl3Yxd5+5qCXQ=;
13, 21 -- Message-ID: {73199.21406.qm-at-web46104.mail.sp1.yahoo.com}
13, 21 -- X-YMail-OSG: jfEhGqsVM1kKXLFSEbAOsUjPcVsR_1Pl9c6zwqicFFbQz_UQ.RvS5x8BGqibxdbzUEbH00LvMYB6otZCl0yb00QzpHPfe6AMTS4WS9.0xPSX9aYWtvFlkNTGIX1kUY5waL3YBqI3vVtivdkGpaWTJ8GwjELVA8D94bK3rn6ljhDIIPOF3UzRnj9bkrGrmXsy8UWRqfvDigJrWHsmIB3r7AXPMjKOb9LXLG6IN5TCFWAWYVQpEPAWwCfmw6e9xAGvSZEsXMUlvH6s.I8_y6F91v_0s86H4oJT6okrww--
13, 21 -- Received: from [132.239.85.200] by web46104.mail.sp1.yahoo.com via HTTP; Thu, 06 Aug 2009 10:14:19 PDT
13, 21 -- X-Mailer: YahooMailClassic/6.1.2 YahooMailWebService/0.7.289.15
13, 21 -- Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT)
13, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 21 -- Subject: Support for core units
13, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
13, 21 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
13, 21 -- MIME-Version: 1.0
13, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================


==============================Original Headers==============================
67, 31 -- From ZZhang-at-uwyo.edu Thu Aug 6 13:36:32 2009
67, 31 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
67, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n76IaWCL019104
67, 31 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 13:36:32 -0500
67, 31 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208])
67, 31 -- by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n76Ia4t5029360
67, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO);
67, 31 -- Thu, 6 Aug 2009 12:36:30 -0600 (MDT)
67, 31 -- (envelope-from ZZhang-at-uwyo.edu)
67, 31 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht1
67, 31 -- ([10.84.60.208]) with mapi; Thu, 6 Aug 2009 12:36:25 -0600
67, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
67, 31 -- To: "vapatpxs-at-yahoo.com" {vapatpxs-at-yahoo.com} ,
67, 31 -- "microscopy-at-microscopy.com"
67, 31 -- {microscopy-at-microscopy.com}
67, 31 -- Date: Thu, 6 Aug 2009 12:36:33 -0600
67, 31 -- Subject: RE: Support for core units
67, 31 -- Thread-Topic: Support for core units
67, 31 -- Thread-Index: AcoWuhGwQIEyFhiISnq+umA4L+ZxjwABCq+A
67, 31 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE7978C64A-at-ponyexpress-mb2}
67, 31 -- References: {200908061719.n76HJQNn009156-at-ns.microscopy.com}
67, 31 -- In-Reply-To: {200908061719.n76HJQNn009156-at-ns.microscopy.com}
67, 31 -- Accept-Language: en-US
67, 31 -- Content-Language: en-US
67, 31 -- X-MS-Has-Attach:
67, 31 -- X-MS-TNEF-Correlator:
67, 31 -- acceptlanguage: en-US
67, 31 -- Content-Type: text/plain; charset="us-ascii"
67, 31 -- MIME-Version: 1.0
67, 31 -- Content-Transfer-Encoding: 8bit
67, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n76IaWCL019104
==============================End of - Headers==============================




From: acalhoun-at-bidmc.harvard.edu
Date: Thu, 6 Aug 2009 13:44:29 -0500
Subject: [Microscopy] viaWWW: Kodak Polycontrast Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both acalhoun-at-bidmc.harvard.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: acalhoun-at-bidmc.harvard.edu
Name: Andrea Calhoun

Organization: Beth Israel Deaconess Medical Center

Title-Subject: [Filtered] Kodak Polycontrast Paper

Question: For any of you out there still using films, enlargers, and
processors, I have 33 boxes of Kodak Professional Polycontrast III RC
black and white paper, 8x10 glossy, that I am looking to give a new
home.

Our EM facility has finally made the jump into the 21st Century and
will no longer be needing this, but it seems a waste just to throw
away. If your lab needs any number of boxes, and is willing to pay
for shipping, please let me know!

Login Host: 134.174.110.5
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Aug 6 13:44:29 2009
7, 11 -- Received: from [146.139.72.120] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n76IiRRP032269
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 13:44:28 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c6a0d4f6c512-at-[172.16.0.101]}
7, 11 -- Date: Thu, 6 Aug 2009 14:44:26 -0400
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: acalhoun-at-bidmc.harvard.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Kodak Polycontrast Paper
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zerfasp-at-ors.od.nih.gov
Date: Thu, 6 Aug 2009 13:45:15 -0500
Subject: [Microscopy] viaWWW: Extended warranty for AMT camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zerfasp-at-ors.od.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: zerfasp-at-ors.od.nih.gov
Name: Patricia Zerfas

Organization: NIH

Title-Subject: [Filtered] Extended warranty for AMT camera

Question: Dear Listservers,
The warranty on the XR611M camera that we purchased from AMT
(Danvers, MA) will expire soon and we have to consider extended
warranties. There are several options to consider with prices
ranging from approx. $700 to $7,000. I have had no problems with the
camera the first year and wanted to know what other laboratories have
done to continue coverage or decided to have no coverage.

Thanks,
Patricia Zerfas

Login Host: 128.231.171.150
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Aug 6 13:45:14 2009
7, 11 -- Received: from [146.139.72.120] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n76IjDQM001392
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 13:45:14 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c6a0d523cf8b-at-[146.139.72.120]}
7, 11 -- Date: Thu, 6 Aug 2009 14:45:13 -0400
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: zerfasp-at-ors.od.nih.gov (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Extended warranty for AMT camera
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dcromey-at-email.arizona.edu
Date: Thu, 6 Aug 2009 13:47:41 -0500
Subject: [Microscopy] Support for core units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You might remind your administrators/accountants that if your facility will
be charging federal grants (USA), then you are subject to the lengthy and
frustrating OMB circular A-21
{http://www.whitehouse.gov/omb/rewrite/circulars/a021/a021.html} . I am not
an expert on this, but I am pasting in the abstract and some notes I took
while attending a presentation at the New England Regional Life Sciences
Core Directors meeting in the fall of 2008. The speaker was Stephen Bobin
(Dartmouth Medical School).

This is not easy.
Doug



OMB Circular A-21 section J47 provides specific guidelines for specialized
service centers. There are two major conditions set forth in this section;
breakeven and non-discriminatory pricing. Crucial to compliance with the
breakeven provision is the correct assessment of the aggregate costs of the
service. It's important to know these costs so that rates can be set to hit
the breakeven point. This requires good accounting and reasonable
predictions of the level of service. Most Universities use an annual
assessment in the form of a rate document. Rate documentation assists in
developing a cost structure from which a price for the service can be
derived. Developing rate documents involves a host of accounting guidelines
from various OMB circulars. It is important to work with your institution
in developing rate documentation to avoid conflicts with the overall F&A
costs (indirects). Discriminatory pricing should be a concern of the
facility manager as it is often difficult to determine the source of funds
for a service request. Cores are often asked for discounts for specially
treated samples.

OMB A-21, A-110 and A-133 can be downloaded from http://www.whitehouse.gov

Rate documentation - describe the services offered, the basis of the rates,
rate calculations used, special pricing, service center policies

Funded depreciation - only for equipment purchased on institutional funds
(not grants), can be included in chargebacks

Discriminatory pricing - federally funded activities can only have one
price! Reduced prices require a subsidy from another account. The Feds
don't care how many accounts are used to pay the bill, as long as the price
is the same.

There should be an institution-wide policy on service centers.

Facilities cannot include in their charges to users the interest on internal
loans. They can expense the interest on external loans.

Unallowable costs include equipment purchase, interest & recreational
alcohol (booze).

If you have multiple similar services where one loses money and the other
service covers those losses, you can address the different pricing for these
services in a policy statement.


^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA

office: AHSC 4212 email: Cromey-at-Arizona.edu
voice: 520-626-2824 fax: 520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


-----Original Message-----
X-from: ZZhang-at-uwyo.edu [mailto:ZZhang-at-uwyo.edu]
Sent: Thursday, August 06, 2009 11:37 AM
To: dcromey-at-email.arizona.edu

Dear Paula:

The following is a forwarded email from Phil Oshel, who did a nation-wide
survey in 2002 on the usage and support of core facilities. Hope this would
help you (and possibly your CEO) get an some idea.

My own experience is that it is almost impossible to support a core facility
solely by user fees. Someone told me that it is as easy as traveling to the
moon.

Whether it is possible to collect $25K of recharge mainly depends on your
user base - how many users and how much time your service is being used. You
don't want to charge users too much (more than $50 per hour on average). It
will chase your users away. With that said, you need at least 500 hours of
usage between now to December in order to collect your $25K, without
bankrupting your users.

I've been asked recently by my administration to "increase the user base, or
recruit more users", so to cover the service contracts solely by user
recharge. We are in a relatively small university, with limited faculty
number and limited funding. Any advice on how and where I could dig out more
users? Anyone out there want to become my user? I have a TEM, a Tabletop
SEM, a confocal, two epi-fluorescence, and in the process of getting a flow
cytometer.....


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038




===============BEGINNING OF FORWARDED EMAIL==========================
-----Original Message-----
X-from: Philip Oshel [mailto:peoshel-at-facstaff.wisc.edu]
Sent: Monday, July 22, 2002 2:45 PM
To: Microscopy-at-sparc5.microscopy.com


It spite of my best efforts to format the survey with Geneva
fixed-width font in ascii, it still came across to some folks (many?)
all disarranged. So Here is the survey reformatted by J Quinn at SUNY
(Stony Brook, I believe).
Phil
**************************************************

2. Nature of institution
COUNT %
Academic 50 89
Other 4 7
Private research 2 4
Commercial 1 2

56 Total Respondents
57 Total Responses

**************************************************

3. Type of facility
COUNT %
Institution core 32 58
Departmental 19 35
Other 5 9
Satellite 1 2

55 Total Respondents
57 Total Responses

**************************************************

4. Predominate work
COUNT %
Biological 53 87
Materials 27 44

61 Total Respondents
80 Total Responses

**************************************************

5. User Base
COUNT %
Multi - user 56 93
Service 47 78

60 Total Respondents
103 Total Responses

**************************************************

6. Type of microscopes
COUNT %
TEM 48 80
SEM 36 60
Optical 18 30
Confocal 18 30
Other 18 30
Other Optical 16 27
FESEM 12 20
ESEM or LV 12 20
Other Confocal 5 8
Scanning Probe 5 8
FETEM 3 5
Other Scanning Probe 3 5

60 Total Respondents
194 Total Responses

**************************************************

7. Salaries
COUNT %
100% Your Institution 31 50
80% Your Institution 6 10
50% Your Institution 5 8
100% User fees 4 6
20% Grants 4 6
100% Grants 4 6
10% Your Institution 3 5
20% User fees 3 5
25% User fees 3 5
30% User fees 3 5
50% User fees 3 5
25% Grants 3 5
70% Your Institution 2 3
75% Your Institution 2 3
10% User fees 2 3
10% Grants 2 3
50% Grants 2 3
Other 2 3
20% Your Institution 1 2
30% Your Institution 1 2
60% Your Institution 1 2
90% Your Institution 1 2
Other 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
Other User fee percentage 1 2
30% Grants 1 2
40% Grants 1 2
70% Grants 1 2
75% Grants 1 2
Other Grants 1 2

62 Total Respondents
98 Total Responses

**************************************************

8. Instrument maintenance
COUNT %
100% User fees 19 31
100% Your Institution 18 30
50% Your Institution 6 9
50% Grants 4 6
10% Your Institution 3 5
90% Your Institution 3 5
50% User fees 3 5
60% User fees 3 5
20% Your Institution 2 3
40% Your Institution 2 3
Other 2 3
10% User fees 2 3
80% User fees 2 3
90% User fees 2 3
10% Grants 2 3
20% Grants 2 3
100% Grants 2 3
25% Your Institution 1 2
30% Your Institution 1 2
25% User fees 1 2
30% User fees 1 2
60% Grants 1 2
75% Grants 1 2
Other 1 2
61 Total Respondents
84 Total Responses

**************************************************

9. Replacement of existing equipment
COUNT %
100% Grants 16 30
100% Your Institution 11 20
50% Your Institution 6 11
50% Grants 5 9
60% Grants 5 9
20% Your Institution 4 7
30% Your Institution 4 7
10% User fees 4 7
100% User fees 4 7
25% Your Institution 3 5
40% Your Institution 3 5
20% User fees 3 5
50% User fees 3 5
10% Your Institution 2 4
70% Grants 2 4
75% Grants 2 4
90% Grants 2 4
40% User fees 1 2
75% User fees 1 2
80% User fees 1 2
90% User fees 1 2
10% Grants 1 2
40% Grants 1 2
80% Grants 1 2
Other 1 2

56 Total Respondents
87 Total Responses

**************************************************

10. Purchasing of new equipment
COUNT %
100% Grants 21 37
100% Your Institution 9 16
50% Your Institution 6 11
50% Grants 6 11
20% Your Institution 5 9
30% Your Institution 5 9
70% Grants 4 7
20% User fees 3 5
60% Grants 3 5
80% Grants 3 5
10% Your Institution 2 4
25% Your Institution 2 4
40% Your Institution 2 4
75% Your Institution 2 4
10% User fees 2 4
30% User fees 2 4
100% User fees 2 4
10% Grants 2 4
90% Grants 2 4
60% Your Institution 1 2
70% Your Institution 1 2
40% User fees 1 2
25% Grants 1 2
40% Grants 1 2
75% Grants 1 2

57 Total Respondents
89 Total Responses

**************************************************

11. Supplies
COUNT %
100% User fees 28 46
100% Your Institution 9 15
50% Grants 5 8
80% Your Institution 4 7
10% Your Institution 3 5
50% Your Institution 3 5
50% User fees 3 5
90% User fees 3 5
10% Grants 3 5
90% Your Institution 2 3
10% User fees 2 3
30% User fees 2 3
20% Grants 2 3
100% Grants 2 3
20% Your Institution 1 2
25% Your Institution 1 2
30% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
20% User fees 1 2
25% User fees 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
40% Grants 1 2
70% Grants 1 2
90% Grants 1 2
Other 1 2

61 Total Respondents
85 Total Responses

**************************************************

12. Operating expenses
COUNT %
100% User fees 19 40
100% Your Institution 16 33
50% Your Institution 6 12
50% User fees 6 12
100% Grants 3 6
20% Your Institution 1 2
40% Your Institution 1 2
60% Your Institution 1 2
80% Your Institution 1 2
40% User fees 1 2
60% User fees 1 2
80% User fees 1 2
20% Grants 1 2
Other 1 2

48 Total Respondents
59 Total Responses

**************************************************
============END OF FORWARDED EMAIL================

-----Original Message-----
X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com]
Sent: Thursday, August 06, 2009 11:19 AM
To: Z.J. Zhang


Hello Everybody,

I was wondering what type of institutional support y'all have at your
various institutions. I work for a research foundation and they want to
drop my salary and have the researchers grants pay my salary and almost
everything else. I am a fee-for-service lab and the CEO wants to see $25K
in recharges between now and December.

We offer TEM, confocal, histology and cryosectioning. We will be adding
micro-CT and a deconvolution microscope that does FRET and can handle
samples that have been transfected(?) with viral vectors.

Is she being unrealistic? I am only 80% time and the only person down here.
Should I start looking for a new position before she shuts me down? How
many people have had their facilities closed by the people who don't see the
big picture?

Please let me know your experiences.

Thanks,

Paula :-}


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.




==============================Original Headers==============================
13, 21 -- From vapatpxs-at-yahoo.com Thu Aug 6 12:14:19 2009
13, 21 -- Received: from web46104.mail.sp1.yahoo.com
(web46104.mail.sp1.yahoo.com [68.180.199.121])
13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n76HEJW2002954
13, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 12:14:19
-0500
13, 21 -- Received: (qmail 22284 invoked by uid 60001); 6 Aug 2009 17:14:19
-0000
13, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1249578859; bh=0spHjN8fjWVOxrej0aZzLx11kJZuuL17bfRBBWtuI5M=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=yzRN5uAA15f2SbhXc0Z5xeFOYNqeol8+iNxld3ET3PUY7dwp0bYPx+v9GyINX+JtA7bYgOqTEL
IXJFZhyQ2ztXkJiS5MElh0jtUUseJggNuTukMjRUR0yLf0+y4sG3dJKe0xFOP2I/FVtcXJqsCVUE
IsO7sDGGUdp/hcr4DaQGU=
13, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 21 -- s=s1024; d=yahoo.com;
13, 21 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
13, 21 --
b=bNC8PmaoJBb+G+hbT4L/Z2scVN6HqbSOuTp4mGI121M2CfmD80AOm/iHeGG3rjFeTVcoPhgDRt
Swv2DAJt1na2O6VypI9U5vHIq2h7EEb4pKKPUkj18ht3rcUaZTxPCjyRqC+SUFC3AiUTITPfRiAt
1PW+G/I9dl3Yxd5+5qCXQ=;
13, 21 -- Message-ID: {73199.21406.qm-at-web46104.mail.sp1.yahoo.com}
13, 21 -- X-YMail-OSG:
jfEhGqsVM1kKXLFSEbAOsUjPcVsR_1Pl9c6zwqicFFbQz_UQ.RvS5x8BGqibxdbzUEbH00LvMYB6
otZCl0yb00QzpHPfe6AMTS4WS9.0xPSX9aYWtvFlkNTGIX1kUY5waL3YBqI3vVtivdkGpaWTJ8Gw
jELVA8D94bK3rn6ljhDIIPOF3UzRnj9bkrGrmXsy8UWRqfvDigJrWHsmIB3r7AXPMjKOb9LXLG6I
N5TCFWAWYVQpEPAWwCfmw6e9xAGvSZEsXMUlvH6s.I8_y6F91v_0s86H4oJT6okrww--
13, 21 -- Received: from [132.239.85.200] by web46104.mail.sp1.yahoo.com via
HTTP; Thu, 06 Aug 2009 10:14:19 PDT
13, 21 -- X-Mailer: YahooMailClassic/6.1.2 YahooMailWebService/0.7.289.15
13, 21 -- Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT)
13, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 21 -- Subject: Support for core units
13, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
13, 21 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
13, 21 -- MIME-Version: 1.0
13, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================


==============================Original Headers==============================
67, 31 -- From ZZhang-at-uwyo.edu Thu Aug 6 13:36:32 2009
67, 31 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu
[129.72.10.32])
67, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n76IaWCL019104
67, 31 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 13:36:32
-0500
67, 31 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu
[10.84.60.208])
67, 31 -- by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id
n76Ia4t5029360
67, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO);
67, 31 -- Thu, 6 Aug 2009 12:36:30 -0600 (MDT)
67, 31 -- (envelope-from ZZhang-at-uwyo.edu)
67, 31 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by
ponyexpress-ht1
67, 31 -- ([10.84.60.208]) with mapi; Thu, 6 Aug 2009 12:36:25 -0600
67, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
67, 31 -- To: "vapatpxs-at-yahoo.com" {vapatpxs-at-yahoo.com} ,
67, 31 -- "microscopy-at-microscopy.com"
67, 31 -- {microscopy-at-microscopy.com}
67, 31 -- Date: Thu, 6 Aug 2009 12:36:33 -0600
67, 31 -- Subject: RE: Support for core units
67, 31 -- Thread-Topic: Support for core units
67, 31 -- Thread-Index: AcoWuhGwQIEyFhiISnq+umA4L+ZxjwABCq+A
67, 31 -- Message-ID:
{8E12868E4E3D65439AEC1BAAF2BF60BE7978C64A-at-ponyexpress-mb2}
67, 31 -- References: {200908061719.n76HJQNn009156-at-ns.microscopy.com}
67, 31 -- In-Reply-To: {200908061719.n76HJQNn009156-at-ns.microscopy.com}
67, 31 -- Accept-Language: en-US
67, 31 -- Content-Language: en-US
67, 31 -- X-MS-Has-Attach:
67, 31 -- X-MS-TNEF-Correlator:
67, 31 -- acceptlanguage: en-US
67, 31 -- Content-Type: text/plain; charset="us-ascii"
67, 31 -- MIME-Version: 1.0
67, 31 -- Content-Transfer-Encoding: 8bit
67, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n76IaWCL019104
==============================End of - Headers==============================



==============================Original Headers==============================
92, 27 -- From dcromey-at-email.arizona.edu Thu Aug 6 13:47:40 2009
92, 27 -- Received: from mailgator.email.arizona.edu (pacer.email.arizona.edu [128.196.133.172])
92, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n76IleIP008117
92, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 13:47:40 -0500
92, 27 -- Received: from mailgators_amavis (amavis6.email.arizona.edu [10.0.0.209])
92, 27 -- by mailgator.email.arizona.edu (Postfix) with ESMTP id 7A19B33425EE
92, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 11:47:39 -0700 (MST)
92, 27 -- Received: from RASTER (hepatic1.cba.arizona.edu [128.196.157.6])
92, 27 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 083CC10F1FC7
92, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Aug 2009 11:47:36 -0700 (MST)
92, 27 -- Reply-To: {cromey-at-arizona.edu}
92, 27 -- From: "Doug Cromey" {dcromey-at-email.arizona.edu}
92, 27 -- To: "Microscopy listserv" {Microscopy-at-Microscopy.Com}
92, 27 -- References: {200908061837.n76IbD7Q019917-at-ns.microscopy.com}
92, 27 -- In-Reply-To: {200908061837.n76IbD7Q019917-at-ns.microscopy.com}
92, 27 -- Subject: RE: [Microscopy] RE: Support for core units
92, 27 -- Date: Thu, 6 Aug 2009 11:47:37 -0700
92, 27 -- Organization: University of Arizona
92, 27 -- Message-ID: {005201ca16c6$5e8e7310$1bab5930$-at-arizona.edu}
92, 27 -- MIME-Version: 1.0
92, 27 -- Content-Type: text/plain;
92, 27 -- charset="US-ASCII"
92, 27 -- Content-Transfer-Encoding: 7bit
92, 27 -- X-Mailer: Microsoft Office Outlook 12.0
92, 27 -- Thread-Index: AcoWxO0OACEHEENwQ1SPyKWcbaa8BgAABhdA
92, 27 -- Content-Language: en-us
92, 27 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: renaudgeological-at-execulink.com
Date: Thu, 6 Aug 2009 18:58:24 -0500
Subject: [Microscopy] viaWWW: Silver halides and hydroxides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both renaudgeological-at-execulink.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: renaudgeological-at-execulink.com
Name: Jim

Title-Subject: [Filtered] Silver halides and hydroxides

Question: Hello,

I am trying to remove some silver complexes (silver halides and
silver hydroxides) from a rock composed of calcium carbonate
(limestone). I am not trying to extract native silver but the
complexes as they occur in the rock. My goal is to extract these
complexes as a heavy mineral seperate, mount them on a section, and
analyze them via electron microprobe and XRD. Can anyone suggest a
method of extraction that won't breakdown the silver complexes. I
have considered grinding the rock and adding HCl acid (to dissolve
the calcium carbonate) but I'm afraid that will likely destroy the
silver complexes. Any suggestions?

Regards,

Jim.

Login Host: 209.239.5.7
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Aug 6 18:58:24 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n76NwNkW004467
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 18:58:24 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6a11e8fc258-at-[206.69.208.22]}
8, 11 -- Date: Thu, 6 Aug 2009 18:58:23 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: renaudgeological-at-execulink.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Silver halides and hydroxides
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Thu, 6 Aug 2009 21:07:13 -0500
Subject: [Microscopy] Re: viaWWW: Silver halides and hydroxides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Jim,

Maybe you could use a solution of Sodium EDTA at neutral pH. Ag+ does
not have a high affinity for EDTA, so chances are the insoluble
hydroxide and chloride will not dissolve.
I do not know if the Ca++ removal would be fast, but it might be worth
a try.

Cheers

Jan
===
Jan Leunissen
Aurion

On 7/08/2009, at 11:58 AM, renaudgeological-at-execulink.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both renaudgeological-at-execulink.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: renaudgeological-at-execulink.com
} Name: Jim
}
} Title-Subject: [Filtered] Silver halides and hydroxides
}
} Question: Hello,
}
} I am trying to remove some silver complexes (silver halides and
} silver hydroxides) from a rock composed of calcium carbonate
} (limestone). I am not trying to extract native silver but the
} complexes as they occur in the rock. My goal is to extract these
} complexes as a heavy mineral seperate, mount them on a section, and
} analyze them via electron microprobe and XRD. Can anyone suggest a
} method of extraction that won't breakdown the silver complexes. I
} have considered grinding the rock and adding HCl acid (to dissolve
} the calcium carbonate) but I'm afraid that will likely destroy the
} silver complexes. Any suggestions?
}
} Regards,
}
} Jim.
}
} Login Host: 209.239.5.7
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Thu Aug 6 18:58:24 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n76NwNkW004467
} 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 18:58:24
} -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c6a11e8fc258-at-[206.69.208.22]}
} 8, 11 -- Date: Thu, 6 Aug 2009 18:58:23 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: renaudgeological-at-execulink.com (by way of
} MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Silver halides and hydroxides
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
7, 20 -- From leunissen-at-aurion.nl Thu Aug 6 21:07:12 2009
7, 20 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7727Bae021350
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009 21:07:12 -0500
7, 20 -- Received: from [192.168.1.50] (really [125.238.255.65]) by fep01.xtra.co.nz
7, 20 -- with ESMTP
7, 20 -- id {20090807020701.VSXU25157.fep01.xtra.co.nz-at-[192.168.1.50]} ;
7, 20 -- Fri, 7 Aug 2009 14:07:01 +1200
7, 20 -- Cc: microscopy-at-microscopy.com
7, 20 -- Message-Id: {BE17CEEC-8194-4075-ACB5-607A928DAA9C-at-aurion.nl}
7, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl}
7, 20 -- To: renaudgeological-at-execulink.com
7, 20 -- In-Reply-To: {200908062358.n76Nwm84004805-at-ns.microscopy.com}
7, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3)
7, 20 -- Subject: Re: [Microscopy] viaWWW: Silver halides and hydroxides
7, 20 -- Date: Fri, 7 Aug 2009 14:07:00 +1200
7, 20 -- References: {200908062358.n76Nwm84004805-at-ns.microscopy.com}
7, 20 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 7 Aug 2009 06:13:51 -0500
Subject: [Microscopy] Re: viaWWW: Silver halides and hydroxides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jim,
This isn't my area of expertise, but I suspect (weasel words set to ON) you
will need to remove the carbonate ion from solution to keep the equilium
shifting from solid CaCO3 to the dissociated form and keep the Ca ions in
solution. This sounds like buffer chemistery to me. I suspect your answer
will could involve lactic acid or other week acids and a buffered pH
system. I found (http://jds.fass.org/cgi/content/full/87/4/863) on
solubility of Ca lactate. It may spark a few ideas.

(weasel words set to OFF)
Stay safe........
Frank




renaudgeological-at-
execulink.com
To
08/06/2009 08:08 frank_karl-at-lincolnelectric.com
PM cc

Subject
Please respond to [Microscopy] viaWWW: Silver halides
renaudgeological-at- and hydroxides
execulink.com












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both renaudgeological-at-execulink.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: renaudgeological-at-execulink.com
Name: Jim

Title-Subject: [Filtered] Silver halides and hydroxides

Question: Hello,

I am trying to remove some silver complexes (silver halides and
silver hydroxides) from a rock composed of calcium carbonate
(limestone). I am not trying to extract native silver but the
complexes as they occur in the rock. My goal is to extract these
complexes as a heavy mineral seperate, mount them on a section, and
analyze them via electron microprobe and XRD. Can anyone suggest a
method of extraction that won't breakdown the silver complexes. I
have considered grinding the rock and adding HCl acid (to dissolve
the calcium carbonate) but I'm afraid that will likely destroy the
silver complexes. Any suggestions?

Regards,

Jim.

Login Host: 209.239.5.7
---------------------------------------------------------------------------

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Aug 6 18:58:24 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n76NwNkW004467
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2009
18:58:24 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6a11e8fc258-at-[206.69.208.22]}
8, 11 -- Date: Thu, 6 Aug 2009 18:58:23 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: renaudgeological-at-execulink.com (by way of
MicroscopyListserver)
8, 11 -- Subject: viaWWW: Silver halides and hydroxides
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
26, 22 -- From frank_karl-at-lincolnelectric.com Fri Aug 7 06:13:51 2009
26, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
26, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n77BDoJa029181
26, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Aug 2009 06:13:51 -0500
26, 22 -- In-Reply-To: {200908070008.n7708Kag016467-at-ns.microscopy.com}
26, 22 -- Subject: Re: [Microscopy] viaWWW: Silver halides and hydroxides
26, 22 -- To: renaudgeological-at-execulink.com, Microscopy-at-microscopy.com
26, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
26, 22 -- Message-ID: {OF759131F5.26F9C305-ON8525760B.003C7BC4-8525760B.003DA8B2-at-lincolnelectric.com}
26, 22 -- Date: Fri, 7 Aug 2009 07:13:50 -0400
26, 22 -- From: Frank_Karl-at-lincolnelectric.com
26, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
26, 22 -- 07, 2008) at 08/07/2009 07:13:27 AM,
26, 22 -- CD-MIME complete at 08/07/2009 07:13:27 AM,
26, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
26, 22 -- 07, 2008) at 08/07/2009 07:13:27 AM,
26, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
26, 22 -- 07, 2008) at 08/07/2009 07:13:27 AM,
26, 22 -- Serialize complete at 08/07/2009 07:13:27 AM
26, 22 -- MIME-Version: 1.0
26, 22 -- Content-Type: text/plain;
26, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: corvos-at-aol.com
Date: Mon, 10 Aug 2009 08:03:55 -0500
Subject: [Microscopy] viaWWW: Model 283 IR Spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both corvos-at-aol.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: corvos-at-aol.com
Name: Walter Protheroe

Organization: E-MAC

Title-Subject: [Filtered] Model 283 IR Spectrometer

Question: I am assisting a college in acquiring a part for their GCMS and would like to know if anyone can help on this.

They have a Model 283 IR Spectrometer made by Perkin Elmer that is missing the power cable between the electronics and optics section - 283-0173

Please contact the following person if you have the unit that you want to part with, just the cable or have a schematic... Any help would be appreciated...

Dr. Craig Litton
Brazosport College
(979) 230-3507
craig.litton-at-brazosport.edu


Regards,

Walter Protheroe
E-MAC, Inc.
corvos-at-aol.com



Login Host: 205.188.116.65
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Mon Aug 10 08:03:55 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7AD3si0007606
12, 11 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 08:03:54 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c6a5cac1b81a-at-[206.69.208.22]}
12, 11 -- Date: Mon, 10 Aug 2009 08:03:53 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: corvos-at-aol.com (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Model 283 IR Spectrometer
12, 11 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: yu_zongsen-at-yahoo.com
Date: Mon, 10 Aug 2009 11:12:27 -0500
Subject: [Microscopy] beam shower

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

In STEM mode, I learn that using ' beam shower' can eliminate the contamination. However, I don't exactly know how it works, does it mean that intensive electron beams remove the hydrocarbons on the surface of your specimen, or just decompose them ?


regards,


Leo




==============================Original Headers==============================
8, 20 -- From yu_zongsen-at-yahoo.com Mon Aug 10 11:12:27 2009
8, 20 -- Received: from web31804.mail.mud.yahoo.com (web31804.mail.mud.yahoo.com [68.142.207.67])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7AGCQCg013219
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 11:12:27 -0500
8, 20 -- Received: (qmail 28747 invoked by uid 60001); 10 Aug 2009 16:12:26 -0000
8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1249920746; bh=ZLMCOqG9wQpqXTUEtZS1/L+NzP8ZeFQNkh0VIrWtXPw=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=VGqxpitJUIEXbQ0HYhhUl3X0gABEx9Jmc4KgH9oxr5/vgkXlPjerF3SmX3qhBpLiczh/4tYGLjZABACwwKidwU4YzW4b48Q9yUYIaf94IMaxeffFhLt5BgqotSJ4RxBtDkyE8NApu2i4HeU5Sue2c5oJ50oTJXNnApp187DPXWo=
8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 20 -- s=s1024; d=yahoo.com;
8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
8, 20 -- b=0VadSn8olASXBS9NabaRumB0U0jkkkow3xb1nL0KXtdxxoG7TCxFfxvJKqMv0bE/z/TlKK25ncu7UC029McvIx1JgyPdgXwbQEOCdg8x+E0Ja84kzXOvwLrjin5Kt0Hx9Ami2Vdpr8j5puyHy77UKTshMbliaX/6VLTL9OMRBfM=;
8, 20 -- Message-ID: {590527.28605.qm-at-web31804.mail.mud.yahoo.com}
8, 20 -- X-YMail-OSG: vdToAdYVM1lDEF9f.DMYFkoPGFz.XThP9rd3Yat9mcblvih7Zrov9H9RcVnzxtC_6isfLzsEUQX78BfXnuh4Pl732FkCW4C59KKanfYJIf4h7quN54Ym3nFtJiQxxhRutZib.R4wuNHdbMBt4JXVGF9DivPqGf7X_1KIlXAXzPjzPwtq2dtuCV4Ud9B79I7aO_v3m7jzneXP8C3RrHKnFU.ylvS7lFrhjl175vKqu5KzhKLg8dzJZe.TZQP1.Zr16dYgKdGt2TSgRO8NaKuZzn6j2XXYAxbLWOdgW46euhZh0Aa7fhXQeen7Hw8NE.MKFqWbqg--
8, 20 -- Received: from [193.170.20.79] by web31804.mail.mud.yahoo.com via HTTP; Mon, 10 Aug 2009 09:12:26 PDT
8, 20 -- X-Mailer: YahooMailClassic/6.1.2 YahooMailWebService/0.7.347.1
8, 20 -- Date: Mon, 10 Aug 2009 09:12:26 -0700 (PDT)
8, 20 -- From: zongsen yu {yu_zongsen-at-yahoo.com}
8, 20 -- Subject: beam shower
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Mon, 10 Aug 2009 11:27:25 -0500
Subject: [Microscopy] Philips 301 parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have some Philips 301 TEM parts available to anyone willing to pay the
shipping charges. Included are filaments, apertures, specimen holder tips,
plates, receiver boxes, and Wehnelt caps. Let me know if you are
interested.
Ralph Common


==============================Original Headers==============================
3, 31 -- From rcommon-at-msu.edu Mon Aug 10 11:27:25 2009
3, 31 -- Received: from sys02.mail.msu.edu (sys02.mail.msu.edu [35.9.75.102])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7AGRPkr028162
3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 11:27:25 -0500
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=msu.edu;
3, 31 -- s=mail; h=From:To:Subject:Date:Message-ID:MIME-Version:
3, 31 -- Content-Type:Content-Transfer-Encoding; bh=Z2KWqfukFGaULsQCMSb0i
3, 31 -- 1GL82T3sVKn7Q3tzackHlQ=; b=m6YlQz1HYsORg2VT2Hx2Tl7IYp11I8DUy4uvq
3, 31 -- D0xabsXUpdt/24RnsitMbVOCaYLtajp+PnIm/ZJMFUeYQGci5pNO2QS2ChMtpONF
3, 31 -- 8Br/sMk8IhtVZXv3T0WZGxGkLLpTdhLnKOamsZDZCgV36tl2AASNnqKMV3PzpaTn
3, 31 -- jcBjkg=
3, 31 -- Received: from [35.9.122.125] (helo=emlab)
3, 31 -- by sys02.mail.msu.edu with esmtpsa (Exim 4.69 #1)
3, 31 -- (TLSv1:RC4-MD5:128)
3, 31 -- id 1MaXiX-0004Bj-AS
3, 31 -- for microscopy-at-microscopy.com; Mon, 10 Aug 2009 12:27:25 -0400
3, 31 -- From: "Ralph Common" {rcommon-at-msu.edu}
3, 31 -- To: {microscopy-at-microscopy.com}
3, 31 -- Subject: Philips 301 parts
3, 31 -- Date: Mon, 10 Aug 2009 12:26:21 -0400
3, 31 -- Message-ID: {008c01ca19d7$4c63c370$7d7a0923-at-msu.edu}
3, 31 -- MIME-Version: 1.0
3, 31 -- Content-Type: text/plain;
3, 31 -- charset="iso-8859-1"
3, 31 -- Content-Transfer-Encoding: 7bit
3, 31 -- X-Priority: 3 (Normal)
3, 31 -- X-MSMail-Priority: Normal
3, 31 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
3, 31 -- Importance: Normal
3, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933
3, 31 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: tom_pella-at-tedpella.com
Date: Mon, 10 Aug 2009 13:32:18 -0500
Subject: [Microscopy] Bob Thomas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I am looking for Bob Thomas of Thomas Technical Services, an independent
EM Service Engineer in the Southern California area. The last phone
number I have is 714-892-5559 and email of 'tts12-at-juno.com'.

That number is disconnected, and that email is no longer in service.
Also, his cell number that another EM Service Engineer had is also
disconnected.

Does anyone have any more current contact info, or his status?

Thanks.

Tom Pella
Ted Pella, Inc.


==============================Original Headers==============================
2, 20 -- From tom_pella-at-tedpella.com Mon Aug 10 13:32:18 2009
2, 20 -- Received: from PELLA-IS.pelco.biz (connect.tedpella.com [12.7.209.242])
2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7AIWHIc017219
2, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 13:32:18 -0500
2, 20 -- Subject: Bob Thomas
2, 20 -- Date: Mon, 10 Aug 2009 11:32:36 -0700
2, 20 -- Message-ID: {9B9C1DE81B04074EAB662592DB1391E362AA35-at-PELLA-IS.pelco.biz}
2, 20 -- X-MS-Has-Attach:
2, 20 -- MIME-Version: 1.0
2, 20 -- Content-Type: text/plain;
2, 20 -- charset="us-ascii"
2, 20 -- X-MS-TNEF-Correlator:
2, 20 -- Thread-Topic: Bob Thomas
2, 20 -- Thread-Index: AcoZ6O8QXBddJWEGRe+z/5pSh0oFrA==
2, 20 -- Content-class: urn:content-classes:message
2, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
2, 20 -- From: "Tom Pella" {tom_pella-at-tedpella.com}
2, 20 -- To: {Microscopy-at-microscopy.com}
2, 20 -- Content-Transfer-Encoding: 8bit
2, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7AIWHIc017219
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Mon, 10 Aug 2009 14:07:22 -0500
Subject: [Microscopy] Re: beam shower to mitigate contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leo

The "beam shower" sometimes called "beam flooding" trick does not
not eliminate contamination, but rather temporarily binds the
moobile hydrocarbon in a thin layer over the entire area of the beam.
This is usually done in TEM mode with a very large beam (& beam
current). This basically spreads the contamination out over a large
area. At the perimeter of the beam flood zone the contamination may
build up to a thick "ring". This technique is generally only
effective if the contamination is specimen borne and is not the
result of poor vaccuum conditions in your instrument.
In my experience with the newer instruments, most of the
contamination is specimen borne.

After a period of time the contamination will return as any unpinned
hydrocarbon diffuses across the surface of your sample and mobile
species returns to the area where you have pinned the previous
material. This diffusion is not simply thermally driven, but there is
an electrostatic component
which accelerates the process to the vicinity of the probe.

If your contamination is on the specimen, then I would recommend
that you consider plasma cleaning your samples, this generally works
better but of course requires you buy or build an appropriate plasma
unit. The advantages is that it can volatize alot (but not all) of
the mobile hydrocarbons over your whole sample converting the
hydrocarbon to a CO species which is pumped away.

Here are a couple of reference to plasma cleaning.

Plasma Processing of Specimens for Electron Microscopy & Microanalysis
N. J. Zaluzec In Progress in Transmission Electron Microscopy 1:
Concepts and Techniques, ( X. F. Zhang, Z. Zhang eds) Publisher:
Springer-Verlag Berlin Heidelberg New York, Chapter 10, Pages
343-351, (2001)

Applications of Reactive Gas Plasma Cleaning Technology in Minimizing
Contamination of Specimens during Transmission and Analytical
Electron Microscopy
S. P. Roberts, N. J. Zaluzec, S. D. Walck, J. T. Grant
Mat. Res. Soc. Symp Proc., 480, 127-136, (1997)

Reactive Gas Plasma Specimen Processing for Use in Microanalysis and
Imaging in Analytical Electron Microscopy
N. J. Zaluzec, B. J. Kestel, D. Henriks
Microscopy and Microanalysis, 3, S2, 983-984, (1997)

You can also check with the WWW sites of various vendors: XEI, SBT,
SPI, Fischione....
they will also be able to provide similiar info.

I might also mention that IR heating sometimes also works, if you
have such a lamp in your specimen airlock.

Good Luck,

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer (ANL) has licensed plasma cleaning technology
for TEM/SEM
applications to most commerical firms who sell these units.




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 630-252-7901, Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec-at-ANL
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Fellow of the Microscopy Society of America
Visiting Professor of Physics - Northern Illinois University




===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
28, 16 -- From zaluzec-at-aaem.amc.anl.gov Mon Aug 10 14:07:22 2009
28, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
28, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7AJ7LGl032634
28, 16 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 14:07:22 -0500
28, 16 -- Received: from [146.139.72.108] (aem108.amc.anl.gov [146.139.72.108])
28, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id n7AJ7Jh7028736
28, 16 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 14:07:21 -0500
28, 16 -- Mime-Version: 1.0
28, 16 -- Message-Id: {p06240802c6a6178fce5c-at-[146.139.72.108]}
28, 16 -- In-Reply-To: {200908101612.n7AGCRfN013226-at-ns.microscopy.com}
28, 16 -- References: {200908101612.n7AGCRfN013226-at-ns.microscopy.com}
28, 16 -- Date: Mon, 10 Aug 2009 14:07:18 -0500
28, 16 -- To: Microscopy-at-microscopy.com
28, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
28, 16 -- Subject: Re: [Microscopy] beam shower to mitigate contamination
28, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 10 Aug 2009 14:50:27 -0500
Subject: [Microscopy] Carbon coaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone,
Oh! Speak to me collective knowledge of the microscopy community:

We are thinking of purchasing a Cressington 208C turbo carbon coater. We
want to evaporate carbon onto metal to isolated inclusion for EDS analysis
by electro-etching. I'm looking for any experience with turbo pumps, noise
and reliability in a semi-dusty environment. Do I need the rotary stage to
cast strong films? I want to have the ability to coat SEM samples with
gold or Pt/Pd. Is this a reasonable way of accomplishing this. (I'd
rather buy a sputter coater, but it's not in the cards.)

I used to use a Kenny style carbon evaporator to carbon coat SEM samples,
but we bought our carbon coated grids, so I have no experience with carbon
films.

Thanks in advance....

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
7, 21 -- From frank_karl-at-lincolnelectric.com Mon Aug 10 14:50:27 2009
7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7AJoQn8015801
7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 14:50:27 -0500
7, 21 -- Subject: Carbon coaters
7, 21 -- To: Microscopy-at-microscopy.com
7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 21 -- Message-ID: {OFC51F8F07.6678B2FB-ON8525760E.006BACC3-8525760E.006CF673-at-lincolnelectric.com}
7, 21 -- Date: Mon, 10 Aug 2009 15:50:26 -0400
7, 21 -- From: Frank_Karl-at-lincolnelectric.com
7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 08/10/2009 03:50:07 PM,
7, 21 -- CD-MIME complete at 08/10/2009 03:50:07 PM,
7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 08/10/2009 03:50:07 PM,
7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 08/10/2009 03:50:07 PM,
7, 21 -- Serialize complete at 08/10/2009 03:50:07 PM
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Mon, 10 Aug 2009 21:28:56 -0500
Subject: [Microscopy] Re: beam shower to mitigate contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is also another technique that is available for cleaning samples other
than plasma cleaning. This technique is called UVO-Cleaning®. A mercury
lamp provides two wavelengths of light, one that creates activated oxygen
species and the other that breaks hydrocarbon bonds. Under a flow of air or
O2, the decomposed hydrocarbons are flushed away from the surface leaving it
clean. This has been an effective methods for cleaning wafers for
deposition systems for years. South Bay Technology has introduced the
UVO-Cleaner® Station that uses this technology. Not only can you clean your
TEM rod but you can clean your sample as well. The system was designed
primarily to store TEM rods and periodically turn on the lamp to keep the
rods clean, but a short time under the UV lamp will clean a sample. The
system is not as expensive as a plasma cleaner because it does not use a
vacuum system. You can find out more about UVO-Cleaning at the bottom of
the following website:
http://www.jelight.com/uvo-ozone-cleaning.php

Disclaimer: SBT manufactures and sells a Plasma Cleaner. SBT manufactures
and sells the UVO-Cleaner® Station in conjunction with Jelight Company, Inc.
UVO-Cleaner® is a registered trademark of Jelight Company, Inc.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Monday, August 10, 2009 12:14 PM
To: swalck-at-southbaytech.com

Leo

The "beam shower" sometimes called "beam flooding" trick does not
not eliminate contamination, but rather temporarily binds the
moobile hydrocarbon in a thin layer over the entire area of the beam.
This is usually done in TEM mode with a very large beam (& beam
current). This basically spreads the contamination out over a large
area. At the perimeter of the beam flood zone the contamination may
build up to a thick "ring". This technique is generally only
effective if the contamination is specimen borne and is not the
result of poor vaccuum conditions in your instrument.
In my experience with the newer instruments, most of the
contamination is specimen borne.

After a period of time the contamination will return as any unpinned
hydrocarbon diffuses across the surface of your sample and mobile
species returns to the area where you have pinned the previous
material. This diffusion is not simply thermally driven, but there is
an electrostatic component
which accelerates the process to the vicinity of the probe.

If your contamination is on the specimen, then I would recommend
that you consider plasma cleaning your samples, this generally works
better but of course requires you buy or build an appropriate plasma
unit. The advantages is that it can volatize alot (but not all) of
the mobile hydrocarbons over your whole sample converting the
hydrocarbon to a CO species which is pumped away.

Here are a couple of reference to plasma cleaning.

Plasma Processing of Specimens for Electron Microscopy & Microanalysis
N. J. Zaluzec In Progress in Transmission Electron Microscopy 1:
Concepts and Techniques, ( X. F. Zhang, Z. Zhang eds) Publisher:
Springer-Verlag Berlin Heidelberg New York, Chapter 10, Pages
343-351, (2001)

Applications of Reactive Gas Plasma Cleaning Technology in Minimizing
Contamination of Specimens during Transmission and Analytical
Electron Microscopy
S. P. Roberts, N. J. Zaluzec, S. D. Walck, J. T. Grant
Mat. Res. Soc. Symp Proc., 480, 127-136, (1997)

Reactive Gas Plasma Specimen Processing for Use in Microanalysis and
Imaging in Analytical Electron Microscopy
N. J. Zaluzec, B. J. Kestel, D. Henriks
Microscopy and Microanalysis, 3, S2, 983-984, (1997)

You can also check with the WWW sites of various vendors: XEI, SBT,
SPI, Fischione....
they will also be able to provide similiar info.

I might also mention that IR heating sometimes also works, if you
have such a lamp in your specimen airlock.

Good Luck,

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer (ANL) has licensed plasma cleaning technology
for TEM/SEM
applications to most commerical firms who sell these units.




} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 630-252-7901, Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec-at-ANL
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Fellow of the Microscopy Society of America
Visiting Professor of Physics - Northern Illinois University




===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
28, 16 -- From zaluzec-at-aaem.amc.anl.gov Mon Aug 10 14:07:22 2009
28, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
28, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n7AJ7LGl032634
28, 16 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 14:07:22
-0500
28, 16 -- Received: from [146.139.72.108] (aem108.amc.anl.gov
[146.139.72.108])
28, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id
n7AJ7Jh7028736
28, 16 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 14:07:21
-0500
28, 16 -- Mime-Version: 1.0
28, 16 -- Message-Id: {p06240802c6a6178fce5c-at-[146.139.72.108]}
28, 16 -- In-Reply-To: {200908101612.n7AGCRfN013226-at-ns.microscopy.com}
28, 16 -- References: {200908101612.n7AGCRfN013226-at-ns.microscopy.com}
28, 16 -- Date: Mon, 10 Aug 2009 14:07:18 -0500
28, 16 -- To: Microscopy-at-microscopy.com
28, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
28, 16 -- Subject: Re: [Microscopy] beam shower to mitigate contamination
28, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================





==============================Original Headers==============================
41, 30 -- From swalck-at-southbaytech.com Mon Aug 10 21:28:56 2009
41, 30 -- Received: from n25.bullet.mail.mud.yahoo.com (n25.bullet.mail.mud.yahoo.com [68.142.206.220])
41, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7B2SuQh008326
41, 30 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 21:28:56 -0500
41, 30 -- Received: from [68.142.200.221] by n25.bullet.mail.mud.yahoo.com with NNFMP; 11 Aug 2009 02:28:55 -0000
41, 30 -- Received: from [68.142.201.241] by t9.bullet.mud.yahoo.com with NNFMP; 11 Aug 2009 02:28:55 -0000
41, 30 -- Received: from [127.0.0.1] by omp402.mail.mud.yahoo.com with NNFMP; 11 Aug 2009 02:28:55 -0000
41, 30 -- X-Yahoo-Newman-Id: 896598.47202.bm-at-omp402.mail.mud.yahoo.com
41, 30 -- Received: (qmail 74409 invoked from network); 11 Aug 2009 02:28:55 -0000
41, 30 -- Received: from unknown (HELO SWALCKD1) (swalck-at-99.154.21.201 with login)
41, 30 -- by smtp118.plus.mail.sp1.yahoo.com with SMTP; 11 Aug 2009 02:28:55 -0000
41, 30 -- X-Yahoo-SMTP: rolQR_SswBBVziZtd9khRtiR2zI_imwDSS008k3o3DaS
41, 30 -- X-YMail-OSG: ndZmAZMVM1kE_6_.jGLLnh6jFcAp0Mu6q6fHkOghv5wX1Hs.71eLKpP8Vz5h.dYjPehXfYSp6OrWAgmzgVJ_ShVub9lbKM7FRPt6.P.p373MK7COxv6vM7qKytwCR9Ha4t34zZ1d7JgmnxyZalizMOg5d__xP4LmrvuYeOyfnhfV_RbwqLptYuHX1oCDhf9ml1VHyyrzqBnaJy1PLH_3p_OZMjWj_pHzQPIB23ad3x7yYtIQRDQYm0UrYYxJJXLJy19cppYg8lsAG7fGDP4kDoCu2tT6AF.WDY5RJfoxEL26UkcLD5s-
41, 30 -- X-Yahoo-Newman-Property: ymail-3
41, 30 -- From: "Scott Walck" {swalck-at-southbaytech.com}
41, 30 -- To: {Microscopy-at-microscopy.com}
41, 30 -- Cc: {zaluzec-at-aaem.amc.anl.gov}
41, 30 -- References: {200908101914.n7AJEJ4K013760-at-ns.microscopy.com}
41, 30 -- In-Reply-To: {200908101914.n7AJEJ4K013760-at-ns.microscopy.com}
41, 30 -- Subject: RE: [Microscopy] Re: beam shower to mitigate contamination
41, 30 -- Date: Mon, 10 Aug 2009 19:30:46 -0700
41, 30 -- Message-ID: {014f01ca1a2b$bbd6c190$338444b0$-at-com}
41, 30 -- MIME-Version: 1.0
41, 30 -- Content-Type: text/plain;
41, 30 -- charset="iso-8859-1"
41, 30 -- X-Mailer: Microsoft Office Outlook 12.0
41, 30 -- thread-index: AcoZ7sO32Y1+AtJgR4iEiuOCTbkKhQAMH4lQ
41, 30 -- Content-Language: en-us
41, 30 -- Content-Transfer-Encoding: 8bit
41, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7B2SuQh008326
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Mon, 10 Aug 2009 22:08:05 -0500
Subject: [Microscopy] Flu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is extremely difficult to operate an electron microscope while in
the throes of a bout with the flu. Therefore, because of the
current high level of concern about the H1N1 flu, I thought all of
you who read the Microscopy Listserver might be interested in
something that can be done that is simple, inexpensive, and highly
likely to increase resistance to the flu.

If you will use Google to search "Vitamin D and Flu' you will find
an article by Dr. Cannell entitled "Epidemic Flu and Vitamin D" in
which he presents clinical evidence showing that supplemental amounts
of Vitamin D (2000 IU or more per day) can be effective in keeping
people from catching the flu. Other articles indicate that taking
supplemental Vitamin D can also moderate the effects of the flue even
if it is contracted.

Dr. Cannell is a real doctor, and his findings are supported by a
number of other reputable researchers. My doctor said that taking
Vitamin D in the recommended amounts would not be harmful, but could
prove very beneficial.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
4, 15 -- From bigelow-at-umich.edu Mon Aug 10 22:08:05 2009
4, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81])
4, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7B384mU023425
4, 15 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 22:08:05 -0500
4, 15 -- Received: FROM [76.226.102.25] (adsl-76-226-102-25.dsl.sfldmi.sbcglobal.net [76.226.102.25])
4, 15 -- By hackers.mr.itd.umich.edu ID 4A80E094.3917B.23459 ;
4, 15 -- Authuser bigelow;
4, 15 -- 10 Aug 2009 23:08:04 EDT
4, 15 -- Mime-Version: 1.0
4, 15 -- Message-Id: {p06240804c6a68e7230e2-at-[76.226.102.25]}
4, 15 -- Date: Mon, 10 Aug 2009 23:07:38 -0400
4, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
4, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
4, 15 -- Subject: Flu
4, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 10 Aug 2009 22:10:04 -0500
Subject: [Microscopy] Re: beam shower

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One way to do this is to use a mineralogical UV
lamp...outside the SEM/TEM. Very low cost.
It can be done over and over again without
damage. I do this frequently and get good results.

gary g.


At 09:14 AM 8/10/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 20 -- From gary-at-gaugler.com Mon Aug 10 22:10:03 2009
8, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7B3A3U2027544
8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 22:10:03 -0500
8, 20 -- Message-Id: {200908110310.n7B3A3U2027544-at-ns.microscopy.com}
8, 20 -- Received: (qmail 32361 invoked from network); 10 Aug 2009 19:56:54 -0700
8, 20 -- Received: by simscan 1.1.0 ppid: 32358, pid: 32359, t: 0.1065s
8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
8, 20 -- by smtp2 with SMTP; 10 Aug 2009 19:56:53 -0700
8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 20 -- Date: Mon, 10 Aug 2009 20:09:53 -0700
8, 20 -- To: yu_zongsen-at-yahoo.com
8, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
8, 20 -- Subject: Re: [Microscopy] beam shower
8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
8, 20 -- In-Reply-To: {200908101614.n7AGE1tY015605-at-ns.microscopy.com}
8, 20 -- References: {200908101614.n7AGE1tY015605-at-ns.microscopy.com}
8, 20 -- Mime-Version: 1.0
8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 11 Aug 2009 03:02:02 -0500
Subject: [Microscopy] Flu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Interesting subject although not directly related to microscopy.
I've always been said that 5 minutes of sun a day was enough to supply the necessary amount of vitamin D to my body, but there may indeed be a significative difference between "necessary amount" and "really helpful amount".
I have (re)discovered lately Linus Pauling and his theory about supplementation in Vitamin C. I studied the subject for a while before adopting a permanent vitamin cure. Since then I ingest daily my ration of vitamins, one of which is vitamin D (but only 200 I.E.).
After 18 monthes of daily use I cannot say that I saw a difference in susceptibility to upper respiratory illnesses in winter, but I must say that I always was seldom ill. However I did a bad flu last winter which kept me in bed for 4 days with fever, an absolute exceptionnal illness for me and it seems that the vitamin supplementation could do few against it. If it didn't seem to help a lot against infections, I noticed I was much less tired and could better concentrate and better memorize though.

Personally I think that a healthy living already offers a strong protection itself. It may not be a simple solution, but why must solutions necessarily be simple??
We probably spend a lot of time sitting at microscopes, meaning our body didn't move the whole day. For this reason we ought to have some excercice after work. Ingesting tons of vitamins while seating in front of the TV probably does not help much.

BTW here is the last paper of Dr Cannell:

http://www.ncbi.nlm.nih.gov/pubmed/18377099?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum

Best regards,
Stephane

----- Original Message ----
X-from: "bigelow-at-umich.edu" {bigelow-at-umich.edu}
To: nizets2-at-yahoo.com
Sent: Tuesday, August 11, 2009 5:11:32 AM

It is extremely difficult to operate an electron microscope while in
the throes of a bout with the flu.  Therefore, because of the
current high level of concern about the H1N1 flu, I thought all of
you who read the Microscopy Listserver might be interested in
something that can be done that is simple, inexpensive, and highly
likely to increase resistance to the flu.

If you will use Google to search "Vitamin D and Flu'  you will find
an article  by Dr. Cannell entitled "Epidemic Flu and Vitamin D" in
which he presents clinical evidence showing that supplemental amounts
of Vitamin D (2000 IU or more per day) can be effective in keeping
people from catching the flu.  Other articles  indicate that taking
supplemental Vitamin D can also moderate the effects of the flue even
if it is contracted.

  Dr. Cannell is a real doctor, and his findings are supported by a
number of other reputable researchers.  My doctor said that taking
Vitamin D in the recommended amounts would not be harmful, but could
prove very beneficial.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr.,  Univ. of Michigan
Ann Arbor, Michigan 48109-2136
  e-mail: bigelow-at-umich.edu;
  Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor,  MI  48104-6731

==============================Original Headers==============================
4, 15 -- From bigelow-at-umich.edu Mon Aug 10 22:08:05 2009
4, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81])
4, 15 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7B384mU023425
4, 15 --     for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2009 22:08:05 -0500
4, 15 -- Received: FROM [76.226.102.25] (adsl-76-226-102-25.dsl.sfldmi.sbcglobal.net [76.226.102.25])
4, 15 --     By hackers.mr.itd.umich.edu ID 4A80E094.3917B.23459 ;
4, 15 --     Authuser bigelow;
4, 15 --     10 Aug 2009 23:08:04 EDT
4, 15 -- Mime-Version: 1.0
4, 15 -- Message-Id: {p06240804c6a68e7230e2-at-[76.226.102.25]}
4, 15 -- Date: Mon, 10 Aug 2009 23:07:38 -0400
4, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
4, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
4, 15 -- Subject: Flu
4, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
19, 25 -- From nizets2-at-yahoo.com Tue Aug 11 03:02:02 2009
19, 25 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7B822JD030982
19, 25 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 03:02:02 -0500
19, 25 -- Received: (qmail 27689 invoked by uid 60001); 11 Aug 2009 08:02:01 -0000
19, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1249977721; bh=h7EUe7+bnfTHxBo03dHuDltQsMF7lhqvBi4hP8sypa0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=MJbEpruPOpuRrGdc4IiWXYkpPTeZq5k9ZVghOftlRl1YlC3ykqFmNrM3FSse01eY1XjqIE1qa8Q7QFVQgNaXFLHYapQ2qRCKU49gpFqwzNyhjt+oEz9D60mWFnXw72cqMlqapIZBaMCFAWjVtpmHJA0CDemYGvBO7TpuaW1u5MA=
19, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
19, 25 -- s=s1024; d=yahoo.com;
19, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
19, 25 -- b=flFr9i8HCj3CGPELjZsk53sQIqgyPCvUp/ntHIpDP+XKtKuSQYybzksaLWUkyI42OFSiTcgNMFx02VnB6t4xzNHdl+xw5UdqxxyCRaSBkuFBsE04/UVlluilgIrlJrWDkaZXTrvSDo4SsHJv7Ds5Bg04+AyMLStaFcL7cyLMJh4=;
19, 25 -- Message-ID: {507588.20003.qm-at-web110805.mail.gq1.yahoo.com}
19, 25 -- X-YMail-OSG: PoSgIKUVM1nINppnObZuQnYPsZHjIMd8chdpXh8zRUE7jsBKp084G_dA096_W.MLPk1_6s.BDRgUz4uydQBwfUw6WA95Vvqlz0bsI.dN0lgLQmP4xUjboW88uX6MgHAr0E6T72NXfu1l.GG2du9U2j0Kk6Fkr_dkc65J4VDKAjOUA8R_7LHtI8unoUdxnQq9OWsCsjf9rqAqtSX3a30X6w0W9Cj2WMbG1Novlj7QF_OLSeXFipEJs.Opcu4WfERn2EWQrMnQ.5gIWJ3Prk2OylBSkPTPrOV7ucrA7K6oJUC2z9gJlYCtVA9bIMVOk2VEe5Uc758Y9Ulx11CrY1UbFjF6iNzgGRbX1ODImOzk.N0-
19, 25 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Tue, 11 Aug 2009 01:02:00 PDT
19, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
19, 25 -- References: {200908110311.n7B3BWT6032474-at-ns.microscopy.com}
19, 25 -- Date: Tue, 11 Aug 2009 01:02:00 -0700 (PDT)
19, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
19, 25 -- Subject: Re: [Microscopy] Flu
19, 25 -- To: bigelow-at-umich.edu
19, 25 -- Cc: microscopy-at-microscopy.com
19, 25 -- In-Reply-To: {200908110311.n7B3BWT6032474-at-ns.microscopy.com}
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Type: text/plain; charset=iso-8859-1
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7B822JD030982
==============================End of - Headers==============================




From: david_bell-at-millipore.com
Date: Tue, 11 Aug 2009 08:04:36 -0500
Subject: [Microscopy] SEM: Help with preparation of mycoplasma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I have an internal customer who wants images of mycoplasma for sizing
purposes. They prepare the organisms with one of two techniques (see
below for details), yet the results are highly variable. Sometimes we
will get beautiful images of the bugs, other times they appear to be
encased in goo as if they had lysed. Lately it seems there are more of
the latter. I've had them make fresh solutions of everything, with no
success. Being primarily a materials person, I'm at a loss to explain
why, beyond trying the obvious. I was hoping some of you biological
experts out there could take a look at the prep methods they are using and
something would show up as an obvious cause.

Thanks in advance for your help!

David


Long Prep for mycoplasma

Serial dilute mycoplasma culture to achieve desired concentration ( ~ 1 x
105 cfu/ml)
1 ml of culture dilution to 4ml of 5% (v/v) glutaraldehyde
Place samples in refrigerator -at- 4ºC for 60 minutes
Vacuum-filter 2.5ml of fixed solution onto 0.1um Durapore®
Transfer sample to Petri dish. Add 3ml of 5% Glutaraldehyde to cover
filter
Chill -at- 4ºC for 60 minutes
Transfer to new dish, add 3 ml of 1% (w/v) sucrose solution. Chill -at- 4ºC
for 30 minutes
Transfer to new dish and add 3ml 2% (v/v) Osmium tetroxide. Chill -at- 4ºC
for 30 minutes
Rinse 3 times with 3ml Mycoplasma buffer
Transfer to new dish for each dehydration step:
15% EtOH 5min
25% EtOH 5min
35% EtOH 5min
45% EtOH 5min
55% EtOH 5min
65% EtOH 5min
75% EtOH 5min
85% EtOH 5min
95% EtOH 5minmicroscopy-at-microscopy.com
95% EtOH 5min
95% EtOH 5min

Place filters in glass Petri dishes. Add 3ml hexamethyldisilazane for 5
minutes
Allow to dry and desiccate until SEM

Short Prep for mycoplasma

Dilute mycoplasma culture to achieve desired concentration
2ml of culture is added to 3ml glutaraldehyde for 30-60 minutes at room
temperature
Transfer solution to 0.1um Durapore® membrane for filtration
Transfer membrane to semi-dehydration steps:
15% EtOH 5min
25% EtOH 5min
45% EtOH 5min
65% EtOH 5min
85% EtOH 5min
95% EtOH 5min

Bring to SEM for imaging

David Bell
Technical Lead
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108


==============================Original Headers==============================
13, 19 -- From david_bell-at-millipore.com Tue Aug 11 08:04:36 2009
13, 19 -- Received: from mail2.millipore.com (mail2.millipore.com [157.93.11.15])
13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7BD4YhN025831
13, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 08:04:35 -0500
13, 19 -- To: microscopy-at-microscopy.com
13, 19 -- Subject: SEM: Help with preparation of mycoplasma
13, 19 -- MIME-Version: 1.0
13, 19 -- X-KeepSent: D7618220:70D05F09-8525760F:0046DCE7;
13, 19 -- type=4; name=$KeepSent
13, 19 -- X-Mailer: Lotus Notes Release 6.5.6 March 06, 2007
13, 19 -- Message-ID: {OFD7618220.70D05F09-ON8525760F.0046DCE7-8525760F.0047CF61-at-Millipore.com}
13, 19 -- From: David Bell {david_bell-at-millipore.com}
13, 19 -- Date: Tue, 11 Aug 2009 09:04:30 -0400
13, 19 -- X-MIMETrack: Serialize by Router on Mail2/NA/Millipore(Release 8.5 HF345|April 28, 2009) at
13, 19 -- 08/11/2009 09:04:37 AM,
13, 19 -- Serialize complete at 08/11/2009 09:04:37 AM
13, 19 -- Content-Type: text/plain; charset="ISO-8859-1"
13, 19 -- Content-Transfer-Encoding: 8bit
13, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7BD4YhN025831
==============================End of - Headers==============================




From: lorenzo.menzel-at-fiu.edu
Date: Tue, 11 Aug 2009 11:49:08 -0500
Subject: [Microscopy] Re: SEM: Help with preparation of mycoplasma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

The short prep as stated would have a lot of shrinkage of the mycoplasma.

Why not grow some cells (HeLa Cell work great!) on glass coverslips and let
them be infected by the mycoplasma. If these cultures were CPD or
hexamethyldisilazane dried one could see if there was shrinkage of the
tissue cultured cells or not as a control for the mycoplasma shrinkage; the
mycoplasma would be Happy Campers; they would be well stuck and easy to
handle for processing. You do not mention sputter coating which should be
done after the drying step so that the specimens do not have charging
problems.

If you wish a protocol for tissue culture infected with mycoplasma contact
me off line and I¹ll provide one that I use.

I have noted that your address is at Millipore.com so I see why you are
using filters. I¹ll add the following comments:

I personally would use less glutaraldehyde at a 2 to 3% in a phosphate or
sodium cacodylate buffer (not PBS) and filter then instead of waiting. Wash
well in a few changes of buffer then fix in OsO4. Dehydrate then add a
second step of hexamethyldisilazane before air drying.


Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not
represent the NIH. This message is not confidential and can be freely shared
and reproduced.


X-from: {david_bell-at-millipore.com}

Hi David,

In addition to Pat's comments (buffered fixative, lower concentration
of fix), I would add some (3) 100% ethanol changes prior to the HMDS
(2 changes).

You might also try letting the mycoplasma adhere to the membranes
before washing (the membranes), fixation and dehydration.


Lorenzo
On Tue, Aug 11, 2009 at 9:25 AM, {david_bell-at-millipore.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello all,
}
} I have an internal customer who wants images of mycoplasma for sizing
} purposes. They prepare the organisms with one of two techniques (see
} below for details), yet the results are highly variable. Sometimes we
} will get beautiful images of the bugs, other times they appear to be
} encased in goo as if they had lysed. Lately it seems there are more of
} the latter. I've had them make fresh solutions of everything, with no
} success. Being primarily a materials person, I'm at a loss to explain
} why, beyond trying the obvious. I was hoping some of you biological
} experts out there could take a look at the prep methods they are using and
} something would show up as an obvious cause.
}
} Thanks in advance for your help!
}
} David
}
}
} Long Prep for mycoplasma
}
} Serial dilute mycoplasma culture to achieve desired concentration ( ~ 1 x
} 105 cfu/ml)
} 1 ml of culture dilution to 4ml of 5% (v/v) glutaraldehyde
} Place samples in refrigerator -at- 4ºC for 60 minutes
} Vacuum-filter 2.5ml of fixed solution onto 0.1um Durapore®
} Transfer sample to Petri dish. Add 3ml of 5% Glutaraldehyde to cover
} filter
} Chill -at- 4ºC for 60 minutes
} Transfer to new dish, add 3 ml of 1% (w/v) sucrose solution. Chill -at- 4ºC
} for 30 minutes
} Transfer to new dish and add 3ml 2% (v/v) Osmium tetroxide. Chill -at- 4ºC
} for 30 minutes
} Rinse 3 times with 3ml Mycoplasma buffer
} Transfer to new dish for each dehydration step:
} 15% EtOH 5min
} 25% EtOH 5min
} 35% EtOH 5min
} 45% EtOH 5min
} 55% EtOH 5min
} 65% EtOH 5min
} 75% EtOH 5min
} 85% EtOH 5min
} 95% EtOH 5minmicroscopy-at-microscopy.com
} 95% EtOH 5min
} 95% EtOH 5min
}
} Place filters in glass Petri dishes. Add 3ml hexamethyldisilazane for 5
} minutes
} Allow to dry and desiccate until SEM
}
} Short Prep for mycoplasma
}
} Dilute mycoplasma culture to achieve desired concentration
} 2ml of culture is added to 3ml glutaraldehyde for 30-60 minutes at room
} temperature
} Transfer solution to 0.1um Durapore® membrane for filtration
} Transfer membrane to semi-dehydration steps:
} 15% EtOH 5min
} 25% EtOH 5min
} 45% EtOH 5min
} 65% EtOH 5min
} 85% EtOH 5min
} 95% EtOH 5min
}
} Bring to SEM for imaging
}
} David Bell
} Technical Lead
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108


==============================Original Headers==============================
6, 19 -- From lmenz001-at-fiu.edu Tue Aug 11 11:49:08 2009
6, 19 -- Received: from mail-bw0-f222.google.com (mail-bw0-f222.google.com [209.85.218.222])
6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7BGn8W3029158
6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 11:49:08 -0500
6, 19 -- Received: by bwz22 with SMTP id 22so3371897bwz.18
6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 09:49:07 -0700 (PDT)
6, 19 -- MIME-Version: 1.0
6, 19 -- Received: by 10.103.160.3 with SMTP id m3mr2612489muo.3.1250009347238; Tue, 11
6, 19 -- Aug 2009 09:49:07 -0700 (PDT)
6, 19 -- In-Reply-To: {200908111325.n7BDPMXt006085-at-ns.microscopy.com}
6, 19 -- References: {200908111325.n7BDPMXt006085-at-ns.microscopy.com}
6, 19 -- Date: Tue, 11 Aug 2009 12:49:07 -0400
6, 19 -- Message-ID: {8ee6d2fb0908110949p725ca17cu13223cac867d01c-at-mail.gmail.com}
6, 19 -- Subject: Re: [Microscopy] SEM: Help with preparation of mycoplasma
6, 19 -- From: Lorenzo Menzel {lorenzo.menzel-at-fiu.edu}
6, 19 -- To: david_bell-at-millipore.com, microscopy-at-microscopy.com
6, 19 -- Content-Type: text/plain; charset=ISO-8859-1
6, 19 -- Content-Transfer-Encoding: 8bit
6, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7BGn8W3029158
==============================End of - Headers==============================




From: Phil.Ahrenkiel-at-sdsmt.edu
Date: Tue, 11 Aug 2009 19:51:42 -0500
Subject: [Microscopy] viaWWW: demo bio-TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Phil.Ahrenkiel-at-sdsmt.edu as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Phil.Ahrenkiel-at-sdsmt.edu
Name: Phil Ahrenkiel

Organization: South Dakota School of Mines & Technology

Title-Subject: [Filtered] demo bio-TEM samples

Question: We are planning an electron-microscopy
workshop for a group of non-experts, covering
about half TEM and half SEM. For TEM, I have
plenty of materials samples to look at (e.g.,
carbon nanotubes, Au nanoparticles), but I expect
the participants will get a bigger kick out of
looking at more familiar things. I would like to
have some TEM sections of natural, bio-materials,
and am wondering if any demo samples are
available for purchase. I havenít found anything
like this through the major vendors. I have
embedded and will try to section a catís whisker,
some lavender, and a leaf, but I donít have any
experience with these things, or tools for
staining. I would welcome any suggestions on
other materials or techniques. Thanks.

For SEM, I think we can find plenty of
entertaining things (e.g., bugs) to analyze.

-Phil
------------------------------------------
Phil Ahrenkiel, Assistant Professor
Nanoscience and Nanoengineering Ph.D. Program
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701
Office: EP 221
Phone: 605-394-5238, Fax: 605-394-2365
Email: Phil.Ahrenkiel-at-sdsmt.edu


Login Host: 151.159.10.46
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Tue Aug 11 19:51:41 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7C0pe9a030904
10, 13 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 19:51:41 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240800c6a7c26b7d33-at-[206.69.208.22]}
10, 13 -- Date: Tue, 11 Aug 2009 19:51:40 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: Phil.Ahrenkiel-at-sdsmt.edu (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: demo bio-TEM samples
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7C0pe9a030904
==============================End of - Headers==============================




From: bmollon-at-gatan.com
Date: Tue, 11 Aug 2009 19:52:28 -0500
Subject: [Microscopy] viaWWW: Lost and Found NoteBook @ MM2009

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bmollon-at-gatan.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bmollon-at-gatan.com
Name: B Mollon

Organization: Gatan, Inc

Title-Subject: [Filtered] Lost and Found MM2009

Question: Did anyone on the listserver lose a note book in the Gatan
booth exhibit area this year? Its contents appear to be significant
enough that someone would miss this. Please contact me for more
info. CONDOR II is labeled on the front cover.

Login Host: 209.3.42.11
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Aug 11 19:52:28 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7C0qPtY031496
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 19:52:28 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6a7c2ad8cca-at-[206.69.208.22]}
6, 11 -- Date: Tue, 11 Aug 2009 19:52:25 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: bmollon-at-gatan.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Lost and Found NoteBook -at- MM2009
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 12 Aug 2009 03:29:01 -0500
Subject: [Microscopy] viaWWW: demo bio-TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Phil!

By definition electron microscopy allow us to see things we cannot see with bare eyes.
So everything looks unfamiliar under a TEM: no relief, everything B/W and most importantly, one only sees a section through a 3D structure!
I think that sections of human organs would be most interesting to observe and not so hard to find, however to understand a minimum what one sees it is necessary to have some background. A transverse section in skin, the wonderful regularity of muscle cells, Intestinal microvilli with globlet cells and synaptic vesicles (brain sections) are striking examples, don't you think? The human body is an eternal source of enjoyment under a TEM! (and sometimes in other situations too but this question is outside the score of this list)

Best regards,
Stephane

 
----- Original Message ----
X-from: "Phil.Ahrenkiel-at-sdsmt.edu" {Phil.Ahrenkiel-at-sdsmt.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, August 12, 2009 2:57:31 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both Phil.Ahrenkiel-at-sdsmt.edu as
well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Phil.Ahrenkiel-at-sdsmt.edu
Name: Phil Ahrenkiel

Organization: South Dakota School of Mines & Technology

Title-Subject: [Filtered] demo bio-TEM samples

Question: We are planning an electron-microscopy
workshop for a group of non-experts, covering
about half TEM and half SEM.  For TEM, I have
plenty of materials samples to look at (e.g.,
carbon nanotubes, Au nanoparticles), but I expect
the participants will get a bigger kick out of
looking at more familiar things. I would like to
have some TEM sections of natural, bio-materials,
and am wondering if any demo samples are
available for purchase. I havenít found anything
like this through the major vendors. I have
embedded and will try to section a catís whisker,
some lavender, and a leaf, but I donít have any
experience with these things, or tools for
staining. I would welcome any suggestions on
other materials or techniques. Thanks.

For SEM, I think we can find plenty of
entertaining things (e.g., bugs) to analyze.

-Phil
------------------------------------------
Phil Ahrenkiel, Assistant Professor
Nanoscience and Nanoengineering Ph.D. Program
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701
Office: EP 221
Phone: 605-394-5238, Fax: 605-394-2365
Email: Phil.Ahrenkiel-at-sdsmt.edu


  Login Host: 151.159.10.46
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Tue Aug 11 19:51:41 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7C0pe9a030904
10, 13 --     for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 19:51:41 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240800c6a7c26b7d33-at-[206.69.208.22]}
10, 13 -- Date: Tue, 11 Aug 2009 19:51:40 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: Phil.Ahrenkiel-at-sdsmt.edu (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: demo bio-TEM samples
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7C0pe9a030904
==============================End of - Headers==============================






==============================Original Headers==============================
23, 25 -- From nizets2-at-yahoo.com Wed Aug 12 03:29:01 2009
23, 25 -- Received: from web110806.mail.gq1.yahoo.com (web110806.mail.gq1.yahoo.com [67.195.13.229])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7C8T15O008826
23, 25 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 03:29:01 -0500
23, 25 -- Received: (qmail 52645 invoked by uid 60001); 12 Aug 2009 08:29:00 -0000
23, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250065740; bh=kd+RggLe5Vt+HC2zHkM7bflfZTYGV6txJNjRkLnQKbA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=IQHaFsCBcwYGmlDvBQp7N8Tr+bkH8R4E/LJ9ccrJEK7QkgbB9EGntQBGs+5Oq6e11+Avdz//ls8aiwYreR5RBvxg24AfFjyxbY0DUj727ZPAFpRCYahkhlt5b9URbOGCII72xMrqRYZW2VbFviewnAIJLMNt1tPZGCCU6uvMLpE=
23, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 25 -- s=s1024; d=yahoo.com;
23, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 25 -- b=X6pEB97pJ0DGIjj/Q2l/qdYV3P5suIB7oEHKew99wa5oMiqRWI3SWconEcZplDPuRmEXO0snTAWHQC3el6cwRFWy8hWm/sRvEhcbHs7myY0HY423yB7oBb/J0hLDCKw7ejQsNPAlO5GGSvcZ2j+R93PsL3eGULg5SvhYKb5m77c=;
23, 25 -- Message-ID: {326186.51512.qm-at-web110806.mail.gq1.yahoo.com}
23, 25 -- X-YMail-OSG: LQ8gftYVM1lAUEYPsUvqZWVcTD2GmQ5SuYLCTZLlvENv.nOiDrglrAqWLLy9USQuqWaevP3sSCJmEM5wNMTSE_7KxsHrhpkItyn3dx6kgPRMtKXO.wPj60_CW79GvOmI.lKiFhmwGPQP8wMs2f_mmxzAD5nL2pxa9NjjySa9ovOi35.N8CSmqywU8TA8AANXJWgcHgciZiiarykbNIesNfHqNO6C3IC24pKXHXuZ0dl1rCbffL19i2rSkrFK2STaypN6MmUvgb9jMg22s0ruRP3UK0FoQRIWBqIh9WWtPSgjjCVdLFPSOiXGi8VAc0_Cn3sVf9UT1yPjxndk3gvo.OykCasaXw--
23, 25 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Wed, 12 Aug 2009 01:29:00 PDT
23, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
23, 25 -- References: {200908120057.n7C0vVJ1011209-at-ns.microscopy.com}
23, 25 -- Date: Wed, 12 Aug 2009 01:29:00 -0700 (PDT)
23, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 25 -- Subject: Re: [Microscopy] viaWWW: demo bio-TEM samples
23, 25 -- To: Phil.Ahrenkiel-at-sdsmt.edu
23, 25 -- Cc: microscopy-at-microscopy.com
23, 25 -- In-Reply-To: {200908120057.n7C0vVJ1011209-at-ns.microscopy.com}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain; charset=iso-8859-1
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7C8T15O008826
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 12 Aug 2009 03:29:11 -0500
Subject: [Microscopy] viaWWW: demo bio-TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Phil!

By definition electron microscopy allow us to see things we cannot see with bare eyes.
So everything looks unfamiliar under a TEM: no relief, everything B/W and most importantly, one only sees a section through a 3D structure!
I think that sections of human organs would be most interesting to observe and not so hard to find, however to understand a minimum what one sees it is necessary to have some background. A transverse section in skin, the wonderful regularity of muscle cells, Intestinal microvilli with globlet cells and synaptic vesicles (brain sections) are striking examples, don't you think? The human body is an eternal source of enjoyment under a TEM! (and sometimes in other situations too but this question is outside the score of this list)

Best regards,
Stephane

 
----- Original Message ----
X-from: "Phil.Ahrenkiel-at-sdsmt.edu" {Phil.Ahrenkiel-at-sdsmt.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, August 12, 2009 2:57:31 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both Phil.Ahrenkiel-at-sdsmt.edu as
well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Phil.Ahrenkiel-at-sdsmt.edu
Name: Phil Ahrenkiel

Organization: South Dakota School of Mines & Technology

Title-Subject: [Filtered] demo bio-TEM samples

Question: We are planning an electron-microscopy
workshop for a group of non-experts, covering
about half TEM and half SEM.  For TEM, I have
plenty of materials samples to look at (e.g.,
carbon nanotubes, Au nanoparticles), but I expect
the participants will get a bigger kick out of
looking at more familiar things. I would like to
have some TEM sections of natural, bio-materials,
and am wondering if any demo samples are
available for purchase. I havenít found anything
like this through the major vendors. I have
embedded and will try to section a catís whisker,
some lavender, and a leaf, but I donít have any
experience with these things, or tools for
staining. I would welcome any suggestions on
other materials or techniques. Thanks.

For SEM, I think we can find plenty of
entertaining things (e.g., bugs) to analyze.

-Phil
------------------------------------------
Phil Ahrenkiel, Assistant Professor
Nanoscience and Nanoengineering Ph.D. Program
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701
Office: EP 221
Phone: 605-394-5238, Fax: 605-394-2365
Email: Phil.Ahrenkiel-at-sdsmt.edu


  Login Host: 151.159.10.46
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Tue Aug 11 19:51:41 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7C0pe9a030904
10, 13 --     for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2009 19:51:41 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240800c6a7c26b7d33-at-[206.69.208.22]}
10, 13 -- Date: Tue, 11 Aug 2009 19:51:40 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: Phil.Ahrenkiel-at-sdsmt.edu (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: demo bio-TEM samples
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7C0pe9a030904
==============================End of - Headers==============================






==============================Original Headers==============================
23, 25 -- From nizets2-at-yahoo.com Wed Aug 12 03:29:11 2009
23, 25 -- Received: from web110809.mail.gq1.yahoo.com (web110809.mail.gq1.yahoo.com [67.195.13.232])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7C8TAH3008930
23, 25 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 03:29:10 -0500
23, 25 -- Received: (qmail 10951 invoked by uid 60001); 12 Aug 2009 08:29:10 -0000
23, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250065750; bh=kd+RggLe5Vt+HC2zHkM7bflfZTYGV6txJNjRkLnQKbA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jdl4H9w/t6pD5dACPsCkmK3agrc3rGxz7FbBKPLUFrGP/IypEV3h1el+7D1bMH0deh0XU47BvlMpv5ax8HIQQwNHPSL+6bVj2jOFEKMp8nnsTNqUUxh4mXROSfnG3fgt5Cl3KNUwx/jnHZUlG8Wx0xY6QmU/J64QX4wYmXQKybQ=
23, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 25 -- s=s1024; d=yahoo.com;
23, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 25 -- b=PYpvfpPmb/qBWYrALu8PbzbNHxYBb67JYgKI2yS2A23HAIZ2PDPYiQLLUHczD8ysmvmd3DJX1Ijt4lqsMaGuwGZNFvgJaJAxpkxCvYTg9LTD2UZgDEjuT8YhTeH+jpGlknIdWTg0MM1Gtl1EyRIvHdLurveOGJ1SBW4fhczZKHc=;
23, 25 -- Message-ID: {55571.9831.qm-at-web110809.mail.gq1.yahoo.com}
23, 25 -- X-YMail-OSG: 4d_LVUEVM1m1_XNrCXWYC1cn.aJznMck5BmOHXSaaoOb3SJ4if4FHWEYaFyvVwaG_znaQ2ibCC9zmEX7pN9.HNpNZE53ZBUtvma6Jy..JvZ7jTvkMojdA25WHTWmZ7Pr6Fp0M1.TEbupi1itv35Kep63_A.bI6JrX3qunaMrvljaY2Yj9qKCW6_DXmYMMa3ortxIArD0XrD97jPoKTKMga869PJ6TqDaJd9RY6cwYcubSYyCib_wwNHSMRtVBWFNvA.bE6KD4s_yQagxILO_gnyUUhLRTMtnWiv9D09y7zJBZDj267WgZpmAGnCjvVprp4FYNdIpVJ_WqAPpkGqpGknAuxWjCQ--
23, 25 -- Received: from [80.122.101.100] by web110809.mail.gq1.yahoo.com via HTTP; Wed, 12 Aug 2009 01:29:09 PDT
23, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
23, 25 -- References: {200908120057.n7C0vVJ1011209-at-ns.microscopy.com}
23, 25 -- Date: Wed, 12 Aug 2009 01:29:09 -0700 (PDT)
23, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 25 -- Subject: Re: [Microscopy] viaWWW: demo bio-TEM samples
23, 25 -- To: Phil.Ahrenkiel-at-sdsmt.edu
23, 25 -- Cc: microscopy-at-microscopy.com
23, 25 -- In-Reply-To: {200908120057.n7C0vVJ1011209-at-ns.microscopy.com}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain; charset=iso-8859-1
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7C8TAH3008930
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Wed, 12 Aug 2009 10:45:50 -0500
Subject: [Microscopy] FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Am I the only microscopist who would like to strangle the person or
persons responsible for "FIB" because of the effect it has on our writing?

When there was debate as to whether we should use EBSP (for electron
backscattering pattern) or EBSD (for electron backscattering
diffraction), the community chose the latter, in part because we can now
write about an “EBSD pattern”, whereas an “EBSP pattern” would be an
“electron backscattering pattern pattern”.

The term FIB is used to refer to an instrument or a technique but a
focused ion beam is neither an instrument nor a technique. So what is
the correct usage? What are we to make of a sentence (from a rather
famous SEM text) which reads: “All of the advantages of FIB in
semiconductor applications....”? If FIB is replaced by “focused ion
beam”, this makes no grammatical sense. Now it is true that, in that
same text, most of the times that “FIB” occurs it is followed by
“microscope” or “instrument” or “system”, etc., but most people writing
about FIB are not so careful about the grammatical sense of what they
are writing.

How did we get into this mess? Can we, even at this late stage, find an
alternative to FIB that is syntactically reasonable?

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
6, 21 -- From jae5-at-lehigh.edu Wed Aug 12 10:45:50 2009
6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CFjn7v023209
6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 10:45:50 -0500
6, 21 -- Received: from [127.0.0.1] (r054008.mat.Lehigh.EDU [128.180.54.8])
6, 21 -- (authenticated bits=0)
6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n7CFjiah031249
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:45:49 -0400
6, 21 -- Message-ID: {4A82E3A9.8060408-at-lehigh.edu}
6, 21 -- Date: Wed, 12 Aug 2009 11:45:45 -0400
6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
6, 21 -- Organization: Lehigh University
6, 21 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
6, 21 -- MIME-Version: 1.0
6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
6, 21 -- Subject: FIB
6, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed
6, 21 -- Content-Transfer-Encoding: 8bit
6, 21 -- X-Virus-Scanned: clamav-milter 0.95.2 at rain.cc.lehigh.edu
6, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 12 Aug 2009 11:22:45 -0500
Subject: [Microscopy] Re: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alwyn,

Find an alternative now? You know how hard it is to kill a FIB.

Phil

} Am I the only microscopist who would like to strangle the person or
} persons responsible for "FIB" because of the effect it has on our writing?
}
} When there was debate as to whether we should use EBSP (for electron
} backscattering pattern) or EBSD (for electron backscattering
} diffraction), the community chose the latter, in part because we can now
} write about an ìEBSD patternî, whereas an ìEBSP patternî would be an
} ìelectron backscattering pattern patternî.
}
} The term FIB is used to refer to an instrument or a technique but a
} focused ion beam is neither an instrument nor a technique. So what is
} the correct usage? What are we to make of a sentence (from a rather
} famous SEM text) which reads: ìAll of the advantages of FIB in
} semiconductor applications....î? If FIB is replaced by ìfocused ion
} beamî, this makes no grammatical sense. Now it is true that, in that
} same text, most of the times that ìFIBî occurs it is followed by
} ìmicroscopeî or ìinstrumentî or ìsystemî, etc., but most people writing
} about FIB are not so careful about the grammatical sense of what they
} are writing.
}
} How did we get into this mess? Can we, even at this late stage, find an
} alternative to FIB that is syntactically reasonable?
}
} --
} ...........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
5, 27 -- From oshel1pe-at-cmich.edu Wed Aug 12 11:22:45 2009
5, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CGMjxn005994
5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:22:45 -0500
5, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id n7CGMfTq010732
5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 12:22:44 -0400
5, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 27 -- Wed, 12 Aug 2009 12:22:41 -0400
5, 27 -- Mime-Version: 1.0
5, 27 -- Message-Id: {f0624080ac6a89c949e21-at-[141.209.160.249]}
5, 27 -- In-Reply-To: {200908121551.n7CFpYYr028975-at-ns.microscopy.com}
5, 27 -- References: {200908121551.n7CFpYYr028975-at-ns.microscopy.com}
5, 27 -- Date: Wed, 12 Aug 2009 12:22:39 -0400
5, 27 -- To: Microscopy-at-microscopy.com
5, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 27 -- Subject: Re: [Microscopy] FIB
5, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
5, 27 -- X-OriginalArrivalTime: 12 Aug 2009 16:22:42.0103 (UTC) FILETIME=[1DEC6C70:01CA1B69]
5, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN)
5, 27 -- X-Spam-Score: -2.60 () [Tag at 5.00] L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
5, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
5, 27 -- X-CanItPRO-Stream: default
5, 27 -- X-Canit-Stats-ID: 16771708 - e4b7be5ee9a3 - 20090812
5, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
5, 27 -- Content-Transfer-Encoding: 8bit
5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7CGMjxn005994
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Wed, 12 Aug 2009 11:58:53 -0500
Subject: [Microscopy] FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{painful pun}
Maybe the people actually doing FIB should be given the task of coming
up with a new term/acronym. How about Localized Ion Etching (LIE).
FIBers should have no problem with being called LIErs!
{/painful pun}

Jim



oshel1pe-at-cmich.edu wrote:
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Alwyn,
}
} Find an alternative now? You know how hard it is to kill a FIB.
}
} Phil
}
}
} } Am I the only microscopist who would like to strangle the person or
} } persons responsible for "FIB" because of the effect it has on our writing?
} }
} } When there was debate as to whether we should use EBSP (for electron
} } backscattering pattern) or EBSD (for electron backscattering
} } diffraction), the community chose the latter, in part because we can now
} } write about an ìEBSD patternî, whereas an ìEBSP patternî would be an
} } ìelectron backscattering pattern patternî.
} }
} } The term FIB is used to refer to an instrument or a technique but a
} } focused ion beam is neither an instrument nor a technique. So what is
} } the correct usage? What are we to make of a sentence (from a rather
} } famous SEM text) which reads: ìAll of the advantages of FIB in
} } semiconductor applications....î? If FIB is replaced by ìfocused ion
} } beamî, this makes no grammatical sense. Now it is true that, in that
} } same text, most of the times that ìFIBî occurs it is followed by
} } ìmicroscopeî or ìinstrumentî or ìsystemî, etc., but most people writing
} } about FIB are not so careful about the grammatical sense of what they
} } are writing.
} }
} } How did we get into this mess? Can we, even at this late stage, find an
} } alternative to FIB that is syntactically reasonable?
} }
} } --
} } ...........
} } Alwyn Eades
} } Department of Materials Science and Engineering
} } Lehigh University
} } 5 East Packer Avenue
} } Bethlehem
} } Pennsylvania 18015-3195
} } Phone 610 758 4231
} } Fax 610 758 4244
} } jae5-at-lehigh.edu
} }
} } ------------------------------------------------------------------------
} }
} }
} } No virus found in this incoming message.
} } Checked by AVG - www.avg.com
} } Version: 8.5.392 / Virus Database: 270.13.52/2298 - Release Date: 08/12/09 06:09:00
} }
} }


--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Sent from my {insert this week's hip device}


==============================Original Headers==============================
12, 20 -- From jehrman-at-mta.ca Wed Aug 12 11:58:52 2009
12, 20 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.204])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CGwqQC021173
12, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:58:52 -0500
12, 20 -- Received: from host-22-194.mta.ca ([138.73.22.194]:50998)
12, 20 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
12, 20 -- with esmtp id 1MbHAn-0001N6-8h (Exim 4.69) for Microscopy-at-microscopy.com
12, 20 -- (return-path {jehrman-at-mta.ca} ); Wed, 12 Aug 2009 13:59:37 -0300
12, 20 -- Message-ID: {4A82F343.8030400-at-mta.ca}
12, 20 -- Date: Wed, 12 Aug 2009 13:52:19 -0300
12, 20 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
12, 20 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
12, 20 -- MIME-Version: 1.0
12, 20 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
12, 20 -- Subject: Re: [Microscopy] Re: FIB
12, 20 -- References: {200908121623.n7CGN5Ur006659-at-ns.microscopy.com}
12, 20 -- In-Reply-To: {200908121623.n7CGN5Ur006659-at-ns.microscopy.com}
12, 20 -- X-Enigmail-Version: 0.96.0
12, 20 -- Content-Type: text/plain; charset=ISO-8859-1
12, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: lgiannuzzi-at-comcast.net
Date: Wed, 12 Aug 2009 13:18:38 -0500
Subject: [Microscopy] RE: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The crazy usage of the acronym "FIB" can be attributed to its exponential
growth (including applications outside of microscopy) over the past 10-15
years. For good or bad, a focused ion beam column, instrument, or
workstation, has been shortened to just "FIB." The term "FIB" has also
erroneously been used to refer to dual platform FIB/SEM instruments. The
term "FIB tomography" was correct when first introduced since this technique
was actually performed on a single column FIB instrument. However, these
days most "FIB tomograms" are actually a collection of serial sections
collected by FIB milling followed by SEM imaging.

Many do not properly identify the type of image acquired with a FIB/SEM.
When originating with the FIB as a source, the image should be properly
referred to as an "ion induced secondary electron (or ion) image." The
acronym ISE has been used of late to describe this type of FIB secondary
electron image.

I agree the FIB usage and nomenclature is a mess, but it would certainly
take more than the FIB police to sort this out. Most of the time I have
tried to use "FIB" to make grammatical sense, but I am sure I have been
guilty of short cuts here or there. I have seen the word "FIB" used as a
noun, verb, or adjective, as in:

I used the FIB to mill that sample.
Go FIB that sample.
I FIB milled that sample.

I have also seen the word FIB conjugated or pluralized, (with or without an
hypotheses) as in FIBed, FIBing, FIBer, FIBs, etc. (A person operating a FIB
is a FIBster or a FIBber). :)

I do not think that this grammatical problem is plagued solely by the FIB
community. It is not going to get any better, especially in the error of
short-hand text messaging. If anyone has reviewed a recent manuscript for
publication of late, then correct use of "FIB" seems to be the least of our
grammatical problems.

Lucille A Giannuzzi

} -----Original Message-----
} From: jae5-at-lehigh.edu [mailto:jae5-at-lehigh.edu]
} Sent: Wednesday, August 12, 2009 11:54 AM
} To: lgiannuzzi-at-comcast.net
} Subject: [Microscopy] FIB
}
}
}
}
} --------------------------------------------------------------------------
} --
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
} Am I the only microscopist who would like to strangle the person or
} persons responsible for "FIB" because of the effect it has on our writing?
}
} When there was debate as to whether we should use EBSP (for electron
} backscattering pattern) or EBSD (for electron backscattering
} diffraction), the community chose the latter, in part because we can now
} write about an "EBSD pattern", whereas an "EBSP pattern" would be an
} "electron backscattering pattern pattern".
}
} The term FIB is used to refer to an instrument or a technique but a
} focused ion beam is neither an instrument nor a technique. So what is
} the correct usage? What are we to make of a sentence (from a rather
} famous SEM text) which reads: "All of the advantages of FIB in
} semiconductor applications...."? If FIB is replaced by "focused ion
} beam", this makes no grammatical sense. Now it is true that, in that
} same text, most of the times that "FIB" occurs it is followed by
} "microscope" or "instrument" or "system", etc., but most people writing
} about FIB are not so careful about the grammatical sense of what they
} are writing.
}
} How did we get into this mess? Can we, even at this late stage, find an
} alternative to FIB that is syntactically reasonable?
}
} --
} ...........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
}
}
} ==============================Original
} Headers==============================
} 6, 21 -- From jae5-at-lehigh.edu Wed Aug 12 10:45:50 2009
} 6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu
} [128.180.2.160])
} 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n7CFjn7v023209
} 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 10:45:50 -
} 0500
} 6, 21 -- Received: from [127.0.0.1] (r054008.mat.Lehigh.EDU
} [128.180.54.8])
} 6, 21 -- (authenticated bits=0)
} 6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id
} n7CFjiah031249
} 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NOT)
} 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:45:49 -
} 0400
} 6, 21 -- Message-ID: {4A82E3A9.8060408-at-lehigh.edu}
} 6, 21 -- Date: Wed, 12 Aug 2009 11:45:45 -0400
} 6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
} 6, 21 -- Organization: Lehigh University
} 6, 21 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
} 6, 21 -- MIME-Version: 1.0
} 6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
} 6, 21 -- Subject: FIB
} 6, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed
} 6, 21 -- Content-Transfer-Encoding: 8bit
} 6, 21 -- X-Virus-Scanned: clamav-milter 0.95.2 at rain.cc.lehigh.edu
} 6, 21 -- X-Virus-Status: Clean
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
9, 24 -- From lgiannuzzi-at-comcast.net Wed Aug 12 13:18:38 2009
9, 24 -- Received: from QMTA07.westchester.pa.mail.comcast.net (qmta07.westchester.pa.mail.comcast.net [76.96.62.64])
9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CIIbYo005408
9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 13:18:38 -0500
9, 24 -- Received: from OMTA02.westchester.pa.mail.comcast.net ([76.96.62.19])
9, 24 -- by QMTA07.westchester.pa.mail.comcast.net with comcast
9, 24 -- id TSk31c0070QuhwU57WJe0Y; Wed, 12 Aug 2009 18:18:38 +0000
9, 24 -- Received: from Lu ([76.101.236.170])
9, 24 -- by OMTA02.westchester.pa.mail.comcast.net with comcast
9, 24 -- id TWJe1c0053hG0sU3NWJemM; Wed, 12 Aug 2009 18:18:38 +0000
9, 24 -- From: "Lucille A. Giannuzzi" {lgiannuzzi-at-comcast.net}
9, 24 -- To: {Microscopy-at-microscopy.com}
9, 24 -- References: {200908121554.n7CFs4i1032227-at-ns.microscopy.com}
9, 24 -- Subject: RE: [Microscopy] FIB
9, 24 -- Date: Wed, 12 Aug 2009 14:18:35 -0400
9, 24 -- Message-ID: {C08C2835308D4B1B86283B920D7BA536-at-Lu}
9, 24 -- MIME-Version: 1.0
9, 24 -- Content-Type: text/plain;
9, 24 -- charset="us-ascii"
9, 24 -- Content-Transfer-Encoding: 7bit
9, 24 -- X-Mailer: Microsoft Office Outlook 11
9, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
9, 24 -- Thread-Index: AcobZR8L1cZ1M+7PST24QhuhOvX4xgAE800g
9, 24 -- In-Reply-To: {200908121554.n7CFs4i1032227-at-ns.microscopy.com}
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Wed, 12 Aug 2009 13:25:07 -0500
Subject: [Microscopy] Re: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

OK, I've got another grammatical question. If we accept FIB as an
instrument, should it be "a FIB" as Phil wrote it or "an FIB"? Same goes
with "a SEM" or "an SEM"? I've secretly struggled with this every time I've
used them in a sentence. Since I say the letters and not the full term, I
use "an". But I don't know for certain what is correct.



-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Wednesday, August 12, 2009 9:29 AM
To: swalck-at-southbaytech.com

Alwyn,

Find an alternative now? You know how hard it is to kill a FIB.

Phil

} Am I the only microscopist who would like to strangle the person or
} persons responsible for "FIB" because of the effect it has on our writing?
}
} When there was debate as to whether we should use EBSP (for electron
} backscattering pattern) or EBSD (for electron backscattering
} diffraction), the community chose the latter, in part because we can now
} write about an ìEBSD patternî, whereas an ìEBSP patternî would be an
} ìelectron backscattering pattern patternî.
}
} The term FIB is used to refer to an instrument or a technique but a
} focused ion beam is neither an instrument nor a technique. So what is
} the correct usage? What are we to make of a sentence (from a rather
} famous SEM text) which reads: ìAll of the advantages of FIB in
} semiconductor applications....î? If FIB is replaced by ìfocused ion
} beamî, this makes no grammatical sense. Now it is true that, in that
} same text, most of the times that ìFIBî occurs it is followed by
} ìmicroscopeî or ìinstrumentî or ìsystemî, etc., but most people writing
} about FIB are not so careful about the grammatical sense of what they
} are writing.
}
} How did we get into this mess? Can we, even at this late stage, find an
} alternative to FIB that is syntactically reasonable?
}
} --
} ...........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
5, 27 -- From oshel1pe-at-cmich.edu Wed Aug 12 11:22:45 2009
5, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n7CGMjxn005994
5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:22:45
-0500
5, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id
n7CGMfTq010732
5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 12:22:44
-0400
5, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by
egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 27 -- Wed, 12 Aug 2009 12:22:41 -0400
5, 27 -- Mime-Version: 1.0
5, 27 -- Message-Id: {f0624080ac6a89c949e21-at-[141.209.160.249]}
5, 27 -- In-Reply-To: {200908121551.n7CFpYYr028975-at-ns.microscopy.com}
5, 27 -- References: {200908121551.n7CFpYYr028975-at-ns.microscopy.com}
5, 27 -- Date: Wed, 12 Aug 2009 12:22:39 -0400
5, 27 -- To: Microscopy-at-microscopy.com
5, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 27 -- Subject: Re: [Microscopy] FIB
5, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
5, 27 -- X-OriginalArrivalTime: 12 Aug 2009 16:22:42.0103 (UTC)
FILETIME=[1DEC6C70:01CA1B69]
5, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN)
5, 27 -- X-Spam-Score: -2.60 () [Tag at 5.00]
L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
5, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount
Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473;
metrocode=513; areacode=989;
http://maps.google.com/maps?q=43.5647,-84.8473&z=6
5, 27 -- X-CanItPRO-Stream: default
5, 27 -- X-Canit-Stats-ID: 16771708 - e4b7be5ee9a3 - 20090812
5, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
5, 27 -- Content-Transfer-Encoding: 8bit
5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n7CGMjxn005994
==============================End of - Headers==============================





==============================Original Headers==============================
19, 30 -- From swalck-at-southbaytech.com Wed Aug 12 13:25:06 2009
19, 30 -- Received: from n12a.bullet.mail.mud.yahoo.com (n12a.bullet.mail.mud.yahoo.com [209.191.125.177])
19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7CIP6O6016185
19, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 13:25:06 -0500
19, 30 -- Received: from [68.142.200.225] by n12.bullet.mail.mud.yahoo.com with NNFMP; 12 Aug 2009 18:25:06 -0000
19, 30 -- Received: from [68.142.201.254] by t6.bullet.mud.yahoo.com with NNFMP; 12 Aug 2009 18:25:05 -0000
19, 30 -- Received: from [127.0.0.1] by omp415.mail.mud.yahoo.com with NNFMP; 12 Aug 2009 18:25:05 -0000
19, 30 -- X-Yahoo-Newman-Id: 973760.28604.bm-at-omp415.mail.mud.yahoo.com
19, 30 -- Received: (qmail 3767 invoked from network); 12 Aug 2009 18:25:05 -0000
19, 30 -- Received: from unknown (HELO SWALCKD1) (swalck-at-99.154.21.201 with login)
19, 30 -- by smtp117.plus.mail.sp1.yahoo.com with SMTP; 12 Aug 2009 18:25:05 -0000
19, 30 -- X-Yahoo-SMTP: rolQR_SswBBVziZtd9khRtiR2zI_imwDSS008k3o3DaS
19, 30 -- X-YMail-OSG: RMLFYhIVM1nQC3C3NHZuCl9noRPX5SWyiriUvnL3vo_N3EHxX3nEWFtuZGE3XfPBwa.od2.Z0srmm7X9emPKpLjDt2K7Yzy8m57Y34tacADMHOK4aj6eTUwqoFb2leayCED0DiZfJuk.hyhFkeEYp4HEIbOp0H6PAuWgBS4xXDrSoPgl4ZGuxCVZ_wkLXRjHd1Gv6J_fE4T.kAw6a9TvDAdBU3DObZsGSx7qHtkQTlShvq5NitxO5QMmjbBfgnDwZzsyF90K1FLCUrIiV2tzBdTmot2AtId.PCfdrkL64hCFZ_LtsSQ-
19, 30 -- X-Yahoo-Newman-Property: ymail-3
19, 30 -- From: "Scott Walck" {swalck-at-southbaytech.com}
19, 30 -- To: {Microscopy-at-microscopy.com}
19, 30 -- Cc: {oshel1pe-at-cmich.edu}
19, 30 -- References: {200908121628.n7CGSotJ019428-at-ns.microscopy.com}
19, 30 -- In-Reply-To: {200908121628.n7CGSotJ019428-at-ns.microscopy.com}
19, 30 -- Subject: RE: [Microscopy] Re: FIB
19, 30 -- Date: Wed, 12 Aug 2009 11:26:58 -0700
19, 30 -- Message-ID: {002d01ca1b7a$7a508da0$6ef1a8e0$-at-com}
19, 30 -- MIME-Version: 1.0
19, 30 -- Content-Type: text/plain;
19, 30 -- charset="iso-8859-1"
19, 30 -- X-Mailer: Microsoft Office Outlook 12.0
19, 30 -- thread-index: Acobafk1c2OtFo2rTsOkxl2z7I7yaQAD7AnA
19, 30 -- Content-Language: en-us
19, 30 -- Content-Transfer-Encoding: 8bit
19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7CIP6O6016185
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 12 Aug 2009 13:40:35 -0500
Subject: [Microscopy] FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If the acronym begins with a vowel or vowel
sound, use "an", if it begins with a consonant or
consonant sound, use "a".
Hence "a SEM", "a FIB", "an EELS" (just to create
another annoying acronym misuse).
Phil

} OK, I've got another grammatical question. If we accept FIB as an
} instrument, should it be "a FIB" as Phil wrote it or "an FIB"? Same goes
} with "a SEM" or "an SEM"? I've secretly struggled with this every time I've
} used them in a sentence. Since I say the letters and not the full term, I
} use "an". But I don't know for certain what is correct.
}
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} Technical Director
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} US Toll Free: 1-800-728-2233
} Tel: (949) 492-2600
} Fax: (949) 492-1499
}
} www.southbaytech.com
} swalck-at-southbaytech.com
}
}
} -----Original Message-----
} From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
} Sent: Wednesday, August 12, 2009 9:29 AM
} To: swalck-at-southbaytech.com
} Subject: [Microscopy] Re: FIB
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
4, 28 -- From oshel1pe-at-cmich.edu Wed Aug 12 13:40:34 2009
4, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CIeYm5001706
4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 13:40:34 -0500
4, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id n7CIePgh026079
4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 14:40:33 -0400
4, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 28 -- Wed, 12 Aug 2009 14:40:29 -0400
4, 28 -- Mime-Version: 1.0
4, 28 -- Message-Id: {f06240800c6a8bc981f2b-at-[141.209.160.249]}
4, 28 -- In-Reply-To: {002d01ca1b7a$7a508da0$6ef1a8e0$-at-com}
4, 28 -- References: {200908121628.n7CGSotJ019428-at-ns.microscopy.com}
4, 28 -- {002d01ca1b7a$7a508da0$6ef1a8e0$-at-com}
4, 28 -- Date: Wed, 12 Aug 2009 14:40:27 -0400
4, 28 -- To: Microscopy-at-microscopy.com
4, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 28 -- Subject: RE: [Microscopy] Re: FIB
4, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
4, 28 -- X-OriginalArrivalTime: 12 Aug 2009 18:40:29.0218 (UTC) FILETIME=[5D821020:01CA1B7C]
4, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN)
4, 28 -- X-Spam-Score: -2.40 () [Tag at 5.00] L_EXCH_MF,L_USD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
4, 28 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 28 -- X-CanItPRO-Stream: default
4, 28 -- X-Canit-Stats-ID: 16779742 - 45f99e130516 - 20090812
4, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
4, 28 -- Content-Transfer-Encoding: 8bit
4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7CIeYm5001706
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 12 Aug 2009 14:10:55 -0500
Subject: [Microscopy] RE: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We must be tired or on vacation, I'm getting this
correction from many folks. I guess I'm more
ophidian when I pronouce "sss"s and less of
vowelist about using "es". Besides, I often use
"sem" as a word instead of pronoucing the letters
"S E M", and choosing between "a sem" and "an S E
M" would continually trip up my speech centers.
Phil

} But "S" is pronounced "es", so should therefore be an SEM, right?
} Andrea
}
} Andrea Blake Brothers
} Senior Scientist, MMCC
} Microscopy & Microanalysis
} Characterization Center
}
} andrea.brothers-at-biovail.com
}
} (703) 480-5879 office
} (703) 480-5943 fax
}
} BIOVAIL
} 3701 Concorde Parkway
} Chantilly, VA 20151
}
} Faith is a fine invention,
} For gentlemen who see;
} But microscopes are prudent
} In an emergency!
}
} Emily Dickinson (1830-1886)
}
} The information contained in this e-mail message
} may be privileged and confidential information
} and is intended only for the use of the
} individual and/or entity identified in the
} address of this message. If the reader of this
} message is not the intended recipient, or an
} employee or agent responsible to deliver it to
} the intended recipient, you are hereby requested
} not to distribute or copy this communication. If
} you have received this communication in error,
} please notify us immediately by calling us
} collect at (703) 480-6000, or by so advising us
} by return e-mail. In this circumstance, we
} request that you delete the original message
} from your system.
}
}
} -----Original Message-----
} From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
} Sent: Wednesday, August 12, 2009 2:42 PM
} To: Andrea Brothers
} Subject: [Microscopy] FIB
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America To
} Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver



==============================Original Headers==============================
4, 29 -- From oshel1pe-at-cmich.edu Wed Aug 12 14:10:55 2009
4, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CJAt80016971
4, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 14:10:55 -0500
4, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id n7CJAC9N029798
4, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 15:10:54 -0400
4, 29 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 29 -- Wed, 12 Aug 2009 15:10:42 -0400
4, 29 -- Mime-Version: 1.0
4, 29 -- Message-Id: {f06240806c6a8c331aae0-at-[141.209.160.249]}
4, 29 -- In-Reply-To:
4, 29 -- {318F1FB9ABD8D842B1C8F555C5322BEA01A17CF9-at-CA0EXC50.biovail.net}
4, 29 -- References: {200908121841.n7CIfmZM004349-at-ns.microscopy.com}
4, 29 -- {318F1FB9ABD8D842B1C8F555C5322BEA01A17CF9-at-CA0EXC50.biovail.net}
4, 29 -- Date: Wed, 12 Aug 2009 15:10:40 -0400
4, 29 -- To: Microscopy-at-microscopy.com
4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 29 -- Subject: RE: [Microscopy] FIB
4, 29 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
4, 29 -- X-OriginalArrivalTime: 12 Aug 2009 19:10:42.0862 (UTC) FILETIME=[96862CE0:01CA1B80]
4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN)
4, 29 -- X-Spam-Score: -2.30 () [Tag at 5.00] L_EXCH_MF,L_USD,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
4, 29 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 29 -- X-CanItPRO-Stream: default
4, 29 -- X-Canit-Stats-ID: 16781786 - 57b71dcbe7b2 - 20090812
4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
4, 29 -- Content-Transfer-Encoding: 8bit
4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7CJAt80016971
==============================End of - Headers==============================




From: lgiannuzzi-at-comcast.net
Date: Wed, 12 Aug 2009 16:36:34 -0500
Subject: [Microscopy] FW: RE: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As evident from my text below, I am often guilty for misuse of the "a" vs.
"an" rule, especially in front of "FIB" because in my head (and in most
speech) I always pronounce the word "fib" instead of the letters in the
acronym "F - I - B."

Lucille

} -----Original Message-----
} From: lgiannuzzi-at-comcast.net [mailto:lgiannuzzi-at-comcast.net]
} Sent: Wednesday, August 12, 2009 2:24 PM
} To: lgiannuzzi-at-comcast.net
} Subject: [Microscopy] RE: FIB
}
}
}
}
} --------------------------------------------------------------------------
} --
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
} The crazy usage of the acronym "FIB" can be attributed to its exponential
} growth (including applications outside of microscopy) over the past 10-15
} years. For good or bad, a focused ion beam column, instrument, or
} workstation, has been shortened to just "FIB." The term "FIB" has also
} erroneously been used to refer to dual platform FIB/SEM instruments. The
} term "FIB tomography" was correct when first introduced since this
} technique
} was actually performed on a single column FIB instrument. However, these
} days most "FIB tomograms" are actually a collection of serial sections
} collected by FIB milling followed by SEM imaging.
}
} Many do not properly identify the type of image acquired with a FIB/SEM.
} When originating with the FIB as a source, the image should be properly
} referred to as an "ion induced secondary electron (or ion) image." The
} acronym ISE has been used of late to describe this type of FIB secondary
} electron image.
}
} I agree the FIB usage and nomenclature is a mess, but it would certainly
} take more than the FIB police to sort this out. Most of the time I have
} tried to use "FIB" to make grammatical sense, but I am sure I have been
} guilty of short cuts here or there. I have seen the word "FIB" used as a
} noun, verb, or adjective, as in:
}
} I used the FIB to mill that sample.
} Go FIB that sample.
} I FIB milled that sample.
}
} I have also seen the word FIB conjugated or pluralized, (with or without
} an
} hypotheses) as in FIBed, FIBing, FIBer, FIBs, etc. (A person operating a
} FIB
} is a FIBster or a FIBber). :)
}
} I do not think that this grammatical problem is plagued solely by the FIB
} community. It is not going to get any better, especially in the error of
} short-hand text messaging. If anyone has reviewed a recent manuscript for
} publication of late, then correct use of "FIB" seems to be the least of
} our
} grammatical problems.
}
} Lucille A Giannuzzi
}
} } -----Original Message-----
} } From: jae5-at-lehigh.edu [mailto:jae5-at-lehigh.edu]
} } Sent: Wednesday, August 12, 2009 11:54 AM
} } To: lgiannuzzi-at-comcast.net
} } Subject: [Microscopy] FIB
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} --
} } --
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} --
} } --
} }
} } Am I the only microscopist who would like to strangle the person or
} } persons responsible for "FIB" because of the effect it has on our
} writing?
} }
} } When there was debate as to whether we should use EBSP (for electron
} } backscattering pattern) or EBSD (for electron backscattering
} } diffraction), the community chose the latter, in part because we can now
} } write about an "EBSD pattern", whereas an "EBSP pattern" would be an
} } "electron backscattering pattern pattern".
} }
} } The term FIB is used to refer to an instrument or a technique but a
} } focused ion beam is neither an instrument nor a technique. So what is
} } the correct usage? What are we to make of a sentence (from a rather
} } famous SEM text) which reads: "All of the advantages of FIB in
} } semiconductor applications...."? If FIB is replaced by "focused ion
} } beam", this makes no grammatical sense. Now it is true that, in that
} } same text, most of the times that "FIB" occurs it is followed by
} } "microscope" or "instrument" or "system", etc., but most people writing
} } about FIB are not so careful about the grammatical sense of what they
} } are writing.
} }
} } How did we get into this mess? Can we, even at this late stage, find an
} } alternative to FIB that is syntactically reasonable?
} }
} } --
} } ...........
} } Alwyn Eades
} } Department of Materials Science and Engineering
} } Lehigh University
} } 5 East Packer Avenue
} } Bethlehem
} } Pennsylvania 18015-3195
} } Phone 610 758 4231
} } Fax 610 758 4244
} } jae5-at-lehigh.edu
} }
} }
} } ==============================Original
} } Headers==============================
} } 6, 21 -- From jae5-at-lehigh.edu Wed Aug 12 10:45:50 2009
} } 6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu
} } [128.180.2.160])
} } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n7CFjn7v023209
} } 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 10:45:50 -
} } 0500
} } 6, 21 -- Received: from [127.0.0.1] (r054008.mat.Lehigh.EDU
} } [128.180.54.8])
} } 6, 21 -- (authenticated bits=0)
} } 6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id
} } n7CFjiah031249
} } 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} } verify=NOT)
} } 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 11:45:49 -
} } 0400
} } 6, 21 -- Message-ID: {4A82E3A9.8060408-at-lehigh.edu}
} } 6, 21 -- Date: Wed, 12 Aug 2009 11:45:45 -0400
} } 6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
} } 6, 21 -- Organization: Lehigh University
} } 6, 21 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
} } 6, 21 -- MIME-Version: 1.0
} } 6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
} } 6, 21 -- Subject: FIB
} } 6, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed
} } 6, 21 -- Content-Transfer-Encoding: 8bit
} } 6, 21 -- X-Virus-Scanned: clamav-milter 0.95.2 at rain.cc.lehigh.edu
} } 6, 21 -- X-Virus-Status: Clean
} } ==============================End of -
} } Headers==============================
}
}
} ==============================Original
} Headers==============================
} 9, 24 -- From lgiannuzzi-at-comcast.net Wed Aug 12 13:18:38 2009
} 9, 24 -- Received: from QMTA07.westchester.pa.mail.comcast.net
} (qmta07.westchester.pa.mail.comcast.net [76.96.62.64])
} 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n7CIIbYo005408
} 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 13:18:38 -
} 0500
} 9, 24 -- Received: from OMTA02.westchester.pa.mail.comcast.net
} ([76.96.62.19])
} 9, 24 -- by QMTA07.westchester.pa.mail.comcast.net with comcast
} 9, 24 -- id TSk31c0070QuhwU57WJe0Y; Wed, 12 Aug 2009 18:18:38 +0000
} 9, 24 -- Received: from Lu ([76.101.236.170])
} 9, 24 -- by OMTA02.westchester.pa.mail.comcast.net with comcast
} 9, 24 -- id TWJe1c0053hG0sU3NWJemM; Wed, 12 Aug 2009 18:18:38 +0000
} 9, 24 -- From: "Lucille A. Giannuzzi" {lgiannuzzi-at-comcast.net}
} 9, 24 -- To: {Microscopy-at-microscopy.com}
} 9, 24 -- References: {200908121554.n7CFs4i1032227-at-ns.microscopy.com}
} 9, 24 -- Subject: RE: [Microscopy] FIB
} 9, 24 -- Date: Wed, 12 Aug 2009 14:18:35 -0400
} 9, 24 -- Message-ID: {C08C2835308D4B1B86283B920D7BA536-at-Lu}
} 9, 24 -- MIME-Version: 1.0
} 9, 24 -- Content-Type: text/plain;
} 9, 24 -- charset="us-ascii"
} 9, 24 -- Content-Transfer-Encoding: 7bit
} 9, 24 -- X-Mailer: Microsoft Office Outlook 11
} 9, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 9, 24 -- Thread-Index: AcobZR8L1cZ1M+7PST24QhuhOvX4xgAE800g
} 9, 24 -- In-Reply-To: {200908121554.n7CFs4i1032227-at-ns.microscopy.com}
} ==============================End of -
} Headers==============================


==============================Original Headers==============================
4, 22 -- From lgiannuzzi-at-comcast.net Wed Aug 12 16:36:33 2009
4, 22 -- Received: from QMTA03.westchester.pa.mail.comcast.net (qmta03.westchester.pa.mail.comcast.net [76.96.62.32])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CLaXUR003556
4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 16:36:33 -0500
4, 22 -- Received: from OMTA17.westchester.pa.mail.comcast.net ([76.96.62.89])
4, 22 -- by QMTA03.westchester.pa.mail.comcast.net with comcast
4, 22 -- id TUsQ1c0051vXlb853ZcK4a; Wed, 12 Aug 2009 21:36:19 +0000
4, 22 -- Received: from Lu ([76.101.236.170])
4, 22 -- by OMTA17.westchester.pa.mail.comcast.net with comcast
4, 22 -- id TZg61c00M3hG0sU3dZg6VE; Wed, 12 Aug 2009 21:40:07 +0000
4, 22 -- From: "Lucille A. Giannuzzi" {lgiannuzzi-at-comcast.net}
4, 22 -- To: {Microscopy-at-microscopy.com}
4, 22 -- Subject: FW: [Microscopy] RE: FIB
4, 22 -- Date: Wed, 12 Aug 2009 17:36:32 -0400
4, 22 -- Message-ID: {013E7211BCCB40ADA26E5B9C6FE9957E-at-Lu}
4, 22 -- MIME-Version: 1.0
4, 22 -- Content-Type: text/plain;
4, 22 -- charset="us-ascii"
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- X-Mailer: Microsoft Office Outlook 11
4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
4, 22 -- Thread-Index: Acobehs2exxG6dcnTUadQzMLiz9V0QAGrjpg
==============================End of - Headers==============================




From: Susan.Trant-at-viha.ca
Date: Wed, 12 Aug 2009 16:47:28 -0500
Subject: [Microscopy] sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone

We have a new renal pathologist at our hospital. The old pathologist did not require higher magnification images. The new pathologist requires images up to and including 100k. I cut at 60nm and use 300 mesh copper grids.

The staining technique that is used before was:
* 30 minutes of 1% uranyl acetate in distilled water
* distilled water rinse
* 10-15 minutes dry time
* 1% lead citrate in distilled water 5 minutes
* dry time 10-15 minutes

This method was adequate for the old system but the contrast was not great. I decided to go to a methanol based uranyl acetate that seems to have great contrast, but now the sections look like they are folding/wrinkling and plain out falling off of the grids.

* 30 minutes 10% uranyl acetate in methanol
* 3 methanol rinses
* dry 15-20 minutes
* 5 minutes in 1% lead citrate
* distilled water rinse

I even tried the Ted Pella system for grid sticks, but got some background junk on the grids.

Any help

Sue




==============================Original Headers==============================
11, 23 -- From Susan.Trant-at-viha.ca Wed Aug 12 16:47:27 2009
11, 23 -- Received: from VICEXFE03.VIHA.CA (smtpout.viha.ca [207.194.133.161])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7CLlRib017790
11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 16:47:27 -0500
11, 23 -- Received: from VICVEX02.VIHA.CA ([10.193.225.5]) by VICEXFE03.VIHA.CA with Microsoft SMTPSVC(6.0.3790.1830);
11, 23 -- Wed, 12 Aug 2009 14:47:27 -0700
11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 23 -- Content-class: urn:content-classes:message
11, 23 -- MIME-Version: 1.0
11, 23 -- Content-Type: text/plain;
11, 23 -- charset="iso-8859-1"
11, 23 -- Subject: sections falling off grids
11, 23 -- Date: Wed, 12 Aug 2009 14:47:26 -0700
11, 23 -- Message-ID: {8AD1AFCC4FE7864284CBA022E89F7D375112F7-at-VICVEX02.VIHA.CA}
11, 23 -- X-MS-Has-Attach:
11, 23 -- X-MS-TNEF-Correlator:
11, 23 -- Thread-Topic: sections falling off grids
11, 23 -- Thread-Index: Acoblnuj4szHorbaTZ+VWUe68y9sIA==
11, 23 -- From: "Trant, Susan" {Susan.Trant-at-viha.ca}
11, 23 -- To: {Microscopy-at-microscopy.com}
11, 23 -- X-OriginalArrivalTime: 12 Aug 2009 21:47:27.0345 (UTC) FILETIME=[7C086610:01CA1B96]
11, 23 -- Content-Transfer-Encoding: 8bit
11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7CLlRib017790
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Wed, 12 Aug 2009 19:29:11 -0500
Subject: [Microscopy] viaWWW: Free JEOL SEM Specimen Mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sue,

I have not used methanol but 1%UA in 50%EtOH - a drop of 2%UA in water then
added a drop of 200 proof Ethanol. Stain for 10 min. wash several times and
then leave the grid in basic water drops until ready to lead stain. My water
is always acidic so I add a bit of NaOH so as not to add acid to the lead
stain.
Reynold¹s Lead, Sato¹s Triple-Lead or Venable & Coggeshall's lead stain are
all basic and work well.

I should mention that my tissues are block stained in 1% aqueous UA
overnight before dehydration.

Why do you dry the grids before lead staining? Wet grids slip easily into
the lead stain and if there has been a slight precipitation on the surface
it is less likely to stick to the wet sections.

If you are recently a member of the Listserv you may not have noted the
procedure of putting the grids with sections into a hot oven (60 degree C)
for 15 min. or longer before staining (from Debby Sherman). This keeps the
sections well stuck on the grids. I use 200 mesh grids and if having
problems the above warming has worked very well. Of course this does not
help eliminate folds etc. that were formed in picking up the sections in the
first place. You can check unstained grids to see if the folds are formed
before staining.
Sometimes I get fewer folds when using a Loop to pick up the sections before
placing them onto a grid.

Feel free to contact me off line if you have further questions.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not
represent the NIH. This message is not confidential and can be freely shared
and reproduced.
=============

X-from: {Susan.Trant-at-viha.ca}
Reply-To: {Susan.Trant-at-viha.ca}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Free JEOL SEM Specimen Mounts

Question: Doing a little lab clean-up and I have a lot (100's) of SEM
Specimen Mounts for a JEOL SEM. We no longer have a JEOL on campus,
so I thought it would be a good idea for me to offer these to anyone
who needs them. They have not been used and I have many sizes.

Here are the measurements of some sizes:

10mm x 10mm & 45 degree angle
15mm dia x 10mm tall
25mm dia x 5mm tall
10mm dia x 10mm dia
8mm dia x 5mm tall

Hope that they can be used....

Cheers, Peggy

Margaret E. Bisher
Electron Microscopy & Histology Core Facility
Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher-at-princeton.edu


Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Wed Aug 12 19:29:11 2009
12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7D0T9wX019272
12, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 19:29:10 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c6a90eb965ee-at-[206.69.208.22]}
12, 11 -- Date: Wed, 12 Aug 2009 19:29:09 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Free JEOL SEM Specimen Mounts
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: yngli-at-ucdavis.edu
Date: Wed, 12 Aug 2009 19:29:42 -0500
Subject: [Microscopy] viaWWW: JEOL2100F operation manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yngli-at-ucdavis.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yngli-at-ucdavis.edu
Name: Ying Li

Organization: University of California Davis

Title-Subject: [Filtered] For JEOL2100F operation manual

Question: Hi,All,
There anybody has the operation manual of JEOL 2100F? If yes, can
you share it to me? Please send to my emailbox
yngli-at-ucdavis.edu.


Best regards!
Ying Li(Y. Li)
------------------------------------------------------------------------------------------------------------
Department of Chemical Engineering and Materials Science
University of California, Davis,
One Shields Avenue,Davis, CA 95616
Phone:530-752-9568; email:yngli-at-ucdavis.edu


Login Host: 169.237.204.112
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Aug 12 19:29:42 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7D0TfQE019974
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 19:29:42 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c6a90ee36fd6-at-[206.69.208.22]}
9, 11 -- Date: Wed, 12 Aug 2009 19:29:41 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: yngli-at-ucdavis.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: JEOL2100F operation manual
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 12 Aug 2009 23:50:22 -0500
Subject: [Microscopy] Re: FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They are probably all true. Fractures of the
English language and the maker's monikers apply.

An SEM=an SEM. A SEM=a scanning electron microscope.

For FIB, I would use FIB tool...as in "I used a
FIB tool." One could replace tool with system.

In the bigger picture, what difference does it make?
Yeah, grammar is important. But with SEM, TEM and FIB,
it is a small circle of interested people. They
know what is what. And they know what it is.

Upper echelons would not know or care about the difference...IMO.

gary g.



At 11:41 AM 8/12/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
14, 22 -- From gary-at-gaugler.com Wed Aug 12 23:50:22 2009
14, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7D4oLTZ024022
14, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 23:50:21 -0500
14, 22 -- Message-Id: {200908130450.n7D4oLTZ024022-at-ns.microscopy.com}
14, 22 -- Received: (qmail 16152 invoked from network); 12 Aug 2009 22:16:23 -0700
14, 22 -- Received: by simscan 1.1.0 ppid: 16144, pid: 16147, t: 0.2193s
14, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
14, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
14, 22 -- by smtp1 with SMTP; 12 Aug 2009 22:16:23 -0700
14, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 22 -- Date: Wed, 12 Aug 2009 21:50:09 -0700
14, 22 -- To: oshel1pe-at-cmich.edu
14, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
14, 22 -- Subject: Re: [Microscopy] FIB
14, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
14, 22 -- In-Reply-To: {200908121841.n7CIffSC004046-at-ns.microscopy.com}
14, 22 -- References: {200908121841.n7CIffSC004046-at-ns.microscopy.com}
14, 22 -- Mime-Version: 1.0
14, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
14, 22 -- Content-Transfer-Encoding: 8bit
14, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7D4oLTZ024022
==============================End of - Headers==============================




From: christopher_r_huntley-at-yahoo.com
Date: Thu, 13 Aug 2009 00:44:01 -0500
Subject: [Microscopy] FEI Quanta 200 FEG Manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,

I'm beginning temp work next week and will be operating an FEI Quanta 200 FEG, which I am fairly unfamiliar with. Does anybody have an electronic version of the operating manual they could email to me? I hoped I could get help from 'fei.com for owners', but couldn't sign up without the serial number. Thanks!

Chris Huntley
christopher_r_huntley-at-yahoo.com




==============================Original Headers==============================
5, 20 -- From christopher_r_huntley-at-yahoo.com Thu Aug 13 00:44:00 2009
5, 20 -- Received: from web35401.mail.mud.yahoo.com (web35401.mail.mud.yahoo.com [66.163.179.110])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7D5i0Ck007307
5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 00:44:00 -0500
5, 20 -- Received: (qmail 25937 invoked by uid 60001); 13 Aug 2009 05:44:00 -0000
5, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250142240; bh=fpNbbeZUKEGJVh0LyMFOHet5z+ymbDwF/sRHgCBEqvc=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=nfqyq/0BQfI6r3F7ISP/6WjIa6YYY61kmISWBgMzeyE14ljVxLNC5s6qGaqosY2eo5wKX8a4j/HRz8yHPmWI67f6NM1emN8X8VBMEyJLA3ElvGbtlElzdJRV/8WqoyFNHVKPrOjXRfrlravVezgTpzClV+JlG7FjS41ETWYiLH8=
5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 20 -- s=s1024; d=yahoo.com;
5, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
5, 20 -- b=j3upTGoqIKdKjgDbM0reo6gMhViGS3D3v61zrK0WLSq/Orch4iJwcBFYxuxz8th3g0v3bykVQIbbxmjIR7zmLDzu9Kcm6nhNplGWpQk8yICrBnClk7T2oTHZwwazsbfPD2MiSrjG7apTRBne0HkBHL7m7Sk+aJUj+8V20i1U1Rc=;
5, 20 -- Message-ID: {339870.24397.qm-at-web35401.mail.mud.yahoo.com}
5, 20 -- X-YMail-OSG: VToVOPwVM1kLQ3uFXr.8p3TlNMYwK3s44U4h1Hf5OV6IAYwXVQF8tM_Odww9ETRvw6EOMGgN2BYeR.be0E1zyW8OkmKNXJAX264jritMejHVreV6W6SjeoOmjEaP.35V50L.H.fhENlYN7PHP_ePdwZBcsSDUnLZCU7IzEUPGZehmy.6gSQjzpd2jqPIA9qYoOWWjRWp1oqmcJBH1_HcTwL1gVTPcuq076wGGp6GE33DH_xufrs2hl4lZTpu3NDbGS1SkfpBg5GgNRsPSLF1.qdjzZ2rINsRhDu2biTladRxT8ulP6spK8OJdnXI_a1wDSAGhbU49I17H4bNOYYTVZganrjeEXFnAwcb8zSV31U-
5, 20 -- Received: from [75.30.244.51] by web35401.mail.mud.yahoo.com via HTTP; Wed, 12 Aug 2009 22:44:00 PDT
5, 20 -- X-Mailer: YahooMailClassic/6.1.2 YahooMailWebService/0.7.338.2
5, 20 -- Date: Wed, 12 Aug 2009 22:44:00 -0700 (PDT)
5, 20 -- From: christopher_r_huntley-at-yahoo.com
5, 20 -- Subject: FEI Quanta 200 FEG Manual?
5, 20 -- To: Microscopy-at-microscopy.com
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: jimmy-at-j3photography.co.uk
Date: Thu, 13 Aug 2009 01:20:57 -0500
Subject: [Microscopy] RE: sections falling off grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sue

Many years ago, I had similar problems. The folding problem was solved by
'stretching' the sections before they were lifted onto the grids (originally
using a cotton wool swab soaked in chloroform, later changed to a heat pen
on health and safety grounds). The sections coming off the grids problem
was improved by cleaning the grids and then storing them in alcohol until
immediately before use.

Best regards,

James Ito


----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hello everyone

We have a new renal pathologist at our hospital. The old pathologist did not
require higher magnification images. The new pathologist requires images up
to and including 100k. I cut at 60nm and use 300 mesh copper grids.

The staining technique that is used before was:
* 30 minutes of 1% uranyl acetate in distilled water
* distilled water rinse
* 10-15 minutes dry time
* 1% lead citrate in distilled water 5 minutes
* dry time 10-15 minutes

This method was adequate for the old system but the contrast was not great.
I decided to go to a methanol based uranyl acetate that seems to have great
contrast, but now the sections look like they are folding/wrinkling and
plain out falling off of the grids.

* 30 minutes 10% uranyl acetate in methanol
* 3 methanol rinses
* dry 15-20 minutes
* 5 minutes in 1% lead citrate
* distilled water rinse

I even tried the Ted Pella system for grid sticks, but got some background
junk on the grids.

Any help

Sue




==============================Original Headers==============================
11, 23 -- From Susan.Trant-at-viha.ca Wed Aug 12 16:47:27 2009
11, 23 -- Received: from VICEXFE03.VIHA.CA (smtpout.viha.ca
[207.194.133.161])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n7CLlRib017790
11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2009 16:47:27
-0500
11, 23 -- Received: from VICVEX02.VIHA.CA ([10.193.225.5]) by
VICEXFE03.VIHA.CA with Microsoft SMTPSVC(6.0.3790.1830);
11, 23 -- Wed, 12 Aug 2009 14:47:27 -0700
11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 23 -- Content-class: urn:content-classes:message
11, 23 -- MIME-Version: 1.0
11, 23 -- Content-Type: text/plain;
11, 23 -- charset="iso-8859-1"
11, 23 -- Subject: sections falling off grids
11, 23 -- Date: Wed, 12 Aug 2009 14:47:26 -0700
11, 23 -- Message-ID:
{8AD1AFCC4FE7864284CBA022E89F7D375112F7-at-VICVEX02.VIHA.CA}
11, 23 -- X-MS-Has-Attach:
11, 23 -- X-MS-TNEF-Correlator:
11, 23 -- Thread-Topic: sections falling off grids
11, 23 -- Thread-Index: Acoblnuj4szHorbaTZ+VWUe68y9sIA==
11, 23 -- From: "Trant, Susan" {Susan.Trant-at-viha.ca}
11, 23 -- To: {Microscopy-at-microscopy.com}
11, 23 -- X-OriginalArrivalTime: 12 Aug 2009 21:47:27.0345 (UTC)
FILETIME=[7C086610:01CA1B96]
11, 23 -- Content-Transfer-Encoding: 8bit
11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n7CLlRib017790
==============================End of - Headers==============================


==============================Original Headers==============================
18, 25 -- From jimmy-at-j3photography.co.uk Thu Aug 13 01:20:57 2009
18, 25 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.186])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7D6KuDf022235
18, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 01:20:56 -0500
18, 25 -- Received: from JamesItoPC (cpc1-broo1-0-0-cust442.renf.cable.ntl.com [86.0.197.187])
18, 25 -- by mrelayeu.kundenserver.de (node=mreu1) with ESMTP (Nemesis)
18, 25 -- id 0MKv1o-1MbTgF06Rc-000pRo; Thu, 13 Aug 2009 08:20:55 +0200
18, 25 -- Reply-To: {jimmy-at-j3photography.co.uk}
18, 25 -- From: "James Ito" {jimmy-at-j3photography.co.uk}
18, 25 -- To: {Microscopy-at-microscopy.com}
18, 25 -- References: {200908122150.n7CLo4Md023657-at-ns.microscopy.com}
18, 25 -- In-Reply-To: {200908122150.n7CLo4Md023657-at-ns.microscopy.com}
18, 25 -- Subject: RE: [Microscopy] sections falling off grids
18, 25 -- Date: Thu, 13 Aug 2009 07:20:58 +0100
18, 25 -- Message-ID: {001401ca1bde$396ced10$ac46c730$-at-co.uk}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain;
18, 25 -- charset="US-ASCII"
18, 25 -- Content-Transfer-Encoding: 7bit
18, 25 -- X-Mailer: Microsoft Office Outlook 12.0
18, 25 -- Thread-Index: AcobltoA4/cBOw8MQGOpan+VrHt4XQARs0Xg
18, 25 -- Content-Language: en-gb
18, 25 -- X-Provags-ID: V01U2FsdGVkX191A4PxRoluSUqCeEji+mX8cDlQw55FR37F781
18, 25 -- cajGgMkEFhB5o/1ZbnFIgHOOsUp0GzAkWcZVmO74pHgRGAL4eg
18, 25 -- w2XyGS0WsZyOFXNsbF7PA==
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Thu, 13 Aug 2009 07:08:15 -0500
Subject: [Microscopy] Re: JEOL stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To whoever posted the message about free JEOL stubs (I fat-fingered the
keyboard this morning and sent your message to the Land of Wind and
Ghosts) I'd be interested in taking them if they're not already spoken
for. I'll pay shipping and whatever other charges necessary.

Thanks,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Sent from my {insert this week's hip device}


==============================Original Headers==============================
8, 18 -- From jehrman-at-mta.ca Thu Aug 13 07:08:15 2009
8, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.204])
8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7DC8ELm017862
8, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 07:08:15 -0500
8, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:49526)
8, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
8, 18 -- with esmtp id 1MbZ7B-00062R-Hg (Exim 4.69) for Microscopy-at-microscopy.com
8, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 13 Aug 2009 09:09:05 -0300
8, 18 -- Message-ID: {4A8400A5.8020903-at-mta.ca}
8, 18 -- Date: Thu, 13 Aug 2009 09:01:41 -0300
8, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
8, 18 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
8, 18 -- MIME-Version: 1.0
8, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
8, 18 -- Subject: Re: JEOL stubs
8, 18 -- X-Enigmail-Version: 0.96.0
8, 18 -- Content-Type: text/plain; charset=ISO-8859-1
8, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: jpflugheber-at-stlawu.edu
Date: Thu, 13 Aug 2009 08:22:47 -0500
Subject: [Microscopy] Phillips 525 and EDAX available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

We are in the process of upgrading our SEM and have available our
Phillips 525 with Falcon EDAX unit. The 525 is currently operational
but not under service contract. The EDAX unit has been maintained under
service contract. If you are interested, please contact me.

Jill Pflugheber
St. Lawrence University
Canton, NY 13617
315-229-5645
{jpflugheber-at-stlawu.edu}

==============================Original Headers==============================
3, 23 -- From jpflugheber-at-stlawu.edu Thu Aug 13 08:22:47 2009
3, 23 -- Received: from mx4.stlawu.edu (mx4.stlawu.edu [69.6.96.24])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7DDMk6V002539
3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 08:22:47 -0500
3, 23 -- Received: from mx4.stlawu.edu (localhost.localdomain [127.0.0.1])
3, 23 -- by localhost (Postfix) with SMTP id 6C775B6D2D
3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 09:22:45 -0400 (EDT)
3, 23 -- Received: from [10.33.121.53] (jpfl1x6h9g1.stlawu.local [10.33.121.53])
3, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
3, 23 -- (No client certificate requested)
3, 23 -- (Authenticated sender: jpfl)
3, 23 -- by mx4.stlawu.edu (Postfix) with ESMTP id 667E9B6D29
3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 09:22:45 -0400 (EDT)
3, 23 -- Message-ID: {4A8413A5.8000202-at-stlawu.edu}
3, 23 -- Date: Thu, 13 Aug 2009 09:22:45 -0400
3, 23 -- From: Jill Pflugheber {jpflugheber-at-stlawu.edu}
3, 23 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
3, 23 -- MIME-Version: 1.0
3, 23 -- To: microscopy-at-microscopy.com
3, 23 -- Subject: Phillips 525 and EDAX available
3, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 23 -- Content-Transfer-Encoding: 7bit
3, 23 -- X-Virus-Scanned-pmx: 5.5.7.378829, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.8.13.130917
==============================End of - Headers==============================




From: hale0007-at-mc.duke.edu
Date: Thu, 13 Aug 2009 11:49:39 -0500
Subject: [Microscopy] Electron Microscopy Technician Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Technician Position Available (Note: Do not reply to
this
email; instead, see contact information below.)

Location: Duke University Medical Center, Durham, NC

REQUIREMENTS: BS degree. US citizen or green card. Prior training and
experience in running electron microscopes, proficiency in cutting
ultrathin
sections and performing negative staining. Knowledge of scientific
laboratory
operation (making solutions, ordering supplies, typing results, keeping
records, etc.). Clinical laboratory and research experience is
advantageous;
note: prior experience in both TEM and ultramicrotomy is required.

Laboratory description: The work force consists of the director and 6 EM
technologists who perform pathology (500 samples/year), virology (1000
samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes?2 with
cryo attachments, plus ancillary specimen preparation equipment.

EM Laboratory web site:
http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain

Job is posted at www.hr.duke.edu - Jobs - External Candidates

Job # 400330062 Molecular Tech IV

Send resume to:
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Laboratory
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Phone: 919 684-3452
Fax: 919 684-3265
Email: saram-at-duke.edu


==============================Original Headers==============================
10, 26 -- From hale0007-at-mc.duke.edu Thu Aug 13 11:49:38 2009
10, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7DGncsP023192
10, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 11:49:38 -0500
10, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54])
10, 26 -- by porthos.duhs.duke.edu (8.14.3/8.14.3) with ESMTP id n7DGncCH188870
10, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
10, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 12:49:39 -0400
10, 26 -- Importance: Normal
10, 26 -- X-Priority: 3 (Normal)
10, 26 -- In-Reply-To:
10, 26 -- References:
10, 26 -- Subject: Electron Microscopy Technician Position Available
10, 26 -- MIME-Version: 1.0
10, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu}
10, 26 -- To: Microscopy-at-microscopy.com
10, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release
10, 26 -- 8.0.2FP1|January 12, 2009) at 08/13/2009 12:49:34,
10, 26 -- MIME-CD complete at 08/13/2009 12:49:34,
10, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release
10, 26 -- 8.0.2|August 07, 2008) at 08/13/2009 12:49:37 PM
10, 26 -- Message-ID: {OF57D349EA.B7B300A4-ON85257611.005C6DC0-85257611.005C6DCE-at-notes.duke.edu}
10, 26 -- Date: Thu, 13 Aug 2009 12:49:34 -0400
10, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2FP1 January 12, 2009
10, 26 -- Content-type: text/plain; charset=US-ASCII
10, 26 -- X-Scanned-By: MIMEDefang 2.67 on 152.16.199.201
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Thu, 13 Aug 2009 16:50:43 -0500
Subject: [Microscopy] Bookshelf cleanout

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've just finished cleaning out my personal bookshelves, and have the
following available, free to a good home:

Kevex Quantex software reference manual, version VI

Kevex Delta class analyzer tutorial, version 5.07

I also have the following three application notes/brochures, if anyone
is interested, else to the recycle bin they go:

TEM cross-sections for the semiconductor industry, Charles Evans & Associates

Quantum, from Kevex Instruments "A Frank discussion about Kevex's
revolutionary new light element x-ray detector"

Kevex APD Advanced Performance Detector

I realize that these might be pretty much useless, but I didn't know
if anyone out there was into the history side of things and wanted
some of these for their collection.

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
10, 31 -- From kraftpiano-at-gmail.com Thu Aug 13 16:50:42 2009
10, 31 -- Received: from mail-bw0-f212.google.com (mail-bw0-f212.google.com [209.85.218.212])
10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7DLogou019343
10, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 16:50:42 -0500
10, 31 -- Received: by bwz8 with SMTP id 8so860413bwz.4
10, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Aug 2009 14:50:41 -0700 (PDT)
10, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 31 -- d=gmail.com; s=gamma;
10, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject
10, 31 -- :from:to:content-type:content-transfer-encoding;
10, 31 -- bh=s5IC4M/GFdvAboTlJuti5qvyUAc2Ou9nz2i21luQJK0=;
10, 31 -- b=PiMXoBoUocDsvfY3FZakX1x+0NQHl4ir5dT9GqQ8+kZitFoas+1jkZuF++vys7oJFo
10, 31 -- bvzc9KJzrSLmwBYLHbn2uoLyJb2ZYKhM1hypNk5SNaDYJQrSF+pAFbmVFTAattwU0CLO
10, 31 -- HS95qYiCDpTSC/OiOdGiyTERYrMmkpqmwL0K4=
10, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
10, 31 -- d=gmail.com; s=gamma;
10, 31 -- h=mime-version:date:message-id:subject:from:to:content-type
10, 31 -- :content-transfer-encoding;
10, 31 -- b=B7+a9m3QU49qf2iKDsb3TnU6VQC4ZFFxD/5cqCcoEyMXwrHAz2EtH3mbVyzMQZ0d2D
10, 31 -- d28e3hkBSMWjDfCTMUQxxpGHj8wvBA0I5xZB56rI+99BMvRFImzzsT821QQ2xy9Igvvf
10, 31 -- JFyNhMDHmGKY/SEhMKpqcbyJyw47tM9SmNVYU=
10, 31 -- MIME-Version: 1.0
10, 31 -- Received: by 10.204.102.133 with SMTP id g5mr846989bko.180.1250200241764; Thu,
10, 31 -- 13 Aug 2009 14:50:41 -0700 (PDT)
10, 31 -- Date: Thu, 13 Aug 2009 17:50:41 -0400
10, 31 -- Message-ID: {25e2b0d20908131450s3405a6f2pee660bc40b1e1484-at-mail.gmail.com}
10, 31 -- Subject: Bookshelf cleanout
10, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com}
10, 31 -- To: microscopy-at-microscopy.com
10, 31 -- Content-Type: text/plain; charset=ISO-8859-1
10, 31 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: ALawrence-at-entomology.msstate.edu
Date: Fri, 14 Aug 2009 08:08:38 -0500
Subject: [Microscopy] Sputter Coater Comparisons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

We are requesting that if anyone has compared and contrasted sputter
coaters in the last fiscal year as part of a purchase to please
contact us with your results and opinions concerning make/model/price.

Please reply off-line to me or

Bill Monroe monroe-at-emcenter.msstate.edu




==============================Original Headers==============================
7, 21 -- From ALawrence-at-entomology.msstate.edu Fri Aug 14 08:08:38 2009
7, 21 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7ED8c9H032175
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 08:08:38 -0500
7, 21 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197])
7, 21 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n7ED8bc1021326
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL)
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 08:08:37 -0500
7, 21 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu
7, 21 -- with Novell_GroupWise; Fri, 14 Aug 2009 08:08:37 -0500
7, 21 -- Message-Id: {4A851B7F.B26A.00E6.0-at-entomology.msstate.edu}
7, 21 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
7, 21 -- Date: Fri, 14 Aug 2009 08:08:32 -0500
7, 21 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu}
7, 21 -- To: {microscopy-at-microscopy.com}
7, 21 -- Subject: Sputter Coater Comparisons
7, 21 -- Mime-Version: 1.0
7, 21 -- Content-Type: text/plain; charset=US-ASCII
7, 21 -- Content-Disposition: inline
7, 21 -- Content-Transfer-Encoding: 8bit
7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7ED8c9H032175
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Fri, 14 Aug 2009 11:54:14 -0500
Subject: [Microscopy] S440 SEM HELP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,
I just picked up a LEO S440 SEM. It runs fine, except, in the area
where the image is supposed to be displayed on the Windows monitor, it
is this putrid green color. I also cannot load Tif files. All of my
cabling is correct, I've double checked. Could there be a software
issue? Anyone with S440 LEO-32 software expertise? Replies off line
would be appreciated. Thanks in advance.

Gary Easton
Scanners Corporation
90 Aileron Court, Suite 6
Westminster, Maryland USA
410.857.7633 x102

SEM Service/Refurbished SEM's Sales



==============================Original Headers==============================
5, 19 -- From garyeaston-at-scannerscorp.com Fri Aug 14 10:42:47 2009
5, 19 -- Received: from omr16.networksolutionsemail.com (omr16.networksolutionsemail.com [205.178.146.66])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7EFgl8V019050
5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 10:42:47 -0500
5, 19 -- Received: from mail.networksolutionsemail.com (ns-omr16.mgt.hosting.dc2.netsol.com [10.49.6.79])
5, 19 -- by omr16.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n7EFggCf030679
5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 11:42:42 -0400
5, 19 -- Received: (qmail 6875 invoked by uid 78); 14 Aug 2009 15:42:41 -0000
5, 19 -- Received: from unknown (HELO ?127.0.0.1?) (72.81.185.48)
5, 19 -- by ns-omr16.lb.hosting.dc2.netsol.com with SMTP; 14 Aug 2009 15:42:41 -0000
5, 19 -- Message-ID: {4A8585E9.1020600-at-scannerscorp.com}
5, 19 -- Date: Fri, 14 Aug 2009 11:42:33 -0400
5, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
5, 19 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
5, 19 -- MIME-Version: 1.0
5, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
5, 19 -- Subject: LEO S440 SEM
5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From daphne-at-ns3x.twi.com.br Fri Aug 14 11:44:55 2009
Return-Path: {daphne-at-ns3x.twi.com.br}
Received: from vps275.ev1servers.net (ev1s-67-15-108-192.theplanet.com [67.15.108.192] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7EGisYp002637
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 14 Aug 2009 11:44:54 -0500
Message-Id: {200908141644.n7EGisYp002637-at-ns.microscopy.com}
Received: (qmail 23788 invoked from network); 14 Aug 2009 06:22:10 -0400
Received: from unknown (HELO User) (41.202.74.202)
by ev1s-67-15-108-171.theplanet.com with SMTP; 14 Aug 2009 06:22:10 -0400
Reply-To: {oliverwab11-at-yahoo.fr}

Hi Listers,
I received my fix already. Thanks to everyone who replied.

Gary



==============================Original Headers==============================
4, 19 -- From garyeaston-at-scannerscorp.com Fri Aug 14 11:54:14 2009
4, 19 -- Received: from omr13.networksolutionsemail.com (omr13.networksolutionsemail.com [205.178.146.63])
4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7EGsBZP002878
4, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 11:54:13 -0500
4, 19 -- Received: from mail.networksolutionsemail.com (ns-omr13.mgt.hosting.dc2.netsol.com [10.49.6.76])
4, 19 -- by omr13.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n7EGs7p7016693
4, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2009 12:54:07 -0400
4, 19 -- Received: (qmail 26226 invoked by uid 78); 14 Aug 2009 16:54:06 -0000
4, 19 -- Received: from unknown (HELO ?127.0.0.1?) (72.81.185.48)
4, 19 -- by ns-omr13.lb.hosting.dc2.netsol.com with SMTP; 14 Aug 2009 16:54:06 -0000
4, 19 -- Message-ID: {4A8596A5.9050901-at-scannerscorp.com}
4, 19 -- Date: Fri, 14 Aug 2009 12:53:57 -0400
4, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
4, 19 -- User-Agent: Thunderbird 2.0.0.22 (Windows/20090605)
4, 19 -- MIME-Version: 1.0
4, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
4, 19 -- Subject: S440 SEM HELP
4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: fsp-at-boselec.com
Date: Tue, 18 Aug 2009 12:04:30 -0500
Subject: [Microscopy] viaWWW: Workshop on FLIM - Fluorescence Lifetime Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Following on very loosely from the 'microscopes in film' thread [e.g. the
sick bay on board Voyager**], the BBC has just started transmitting a good
documentary series* called 'The cell: The hidden kingdom'. I missed the
first episode [of three] showing last Thursday [repeated tonight at 8.00pm
BBC4] but it is available on BBC iPlayer for those microscopists in the UK
who missed it. Sadly iPlayer probably won't function to internet users
beyond the UK, so only the lucky few here can watch Robert Hooke looking at
a sliver of cork through a microscope lens and recording the "pores" or
"cells" he saw. You also get to see through Anton van Leeuwenhoek's simple
microscope with only one lens to view tiny objects bombing about in pond
water [obviously living microscopic things, called 'Animacules' apparently].

http://www.bbc.co.uk/iplayer/episode/b00m425d/The_Cell_The_Hidden_Kingdom

http://www.bbc.co.uk/programmes/b00m425d

There's not much for the metallurgical crowd and their SEMs though. I expect
the 3 part series will cross the pond to the US shortly.

Regards

Keith

*Well the first episode on the history of the cell [and hence the
microscope] finishing up with Pasteur was great.

Last showing episode one: Tue 18 Aug 8.00 pm BBC Four tonight - I'll try and
remember to tape, sorry DVD, it

**http://memory-alpha.org/en/wiki/Sickbay

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: swalck-at-southbaytech.com [mailto:swalck-at-southbaytech.com]
Sent: 16 June 2009 00:46
To: kjmorris-at-well.ox.ac.uk

My two favorites are from the movies, The Andromeda Strain and Predator 2.

In The Andromeda Strain, they took the virus and put it in a Philips EM300
TEM and then instantaneously got a color image and watched it in real time
multiply under the beam. Recall that movie came out in 1971. In Predator
2, the forensic scientist put the claw into the SEM, closed the door, and
instantly got an XEDS spectrum and concluded that the material wasn't from
earth because it didn't match any of the elements.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com

-----Original Message-----
X-from: bnross-at-interchange.ubc.ca [mailto:bnross-at-interchange.ubc.ca]
Sent: Monday, June 15, 2009 3:35 PM
To: swalck-at-southbaytech.com

Keith

yes I saw this on i-player the other day and thought I had missed the
TV run but, as you say, the repeats are on and I hope to catch all
three episodes. I've enjoyed what I saw of the first episode and it's
fascinating just how much of the original instrumentation was produced
by gifted amateurs rather than people working with optics. Photographs
in textbooks and journals just can't do justice to some of the early
microscopy and such works as Micrographia.

Cheers

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both fsp-at-boselec.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: fsp-at-boselec.com
Name: Fred Perry

Organization: Boston Electronics Corporation

Title-Subject: [Filtered] Workshop on FLIM - Fluorescence Lifetime
Imaging Microscopy

Question: Please join us for the 3rd annual exploration of advanced
TCSPC techniques using Becker & Hickl TCSPC modules. TCSPC is a
technique of interest for those working with:

FLIM Microscopy
Clinical FLIM Applications
STED-FLIM
FRET
Protein Interaction
Tissue Autofluorescence
Single-Molecule Spectroscopy
Single-Molecule FRET
Time-Domain Diffuse Imaging
Transient Fuorescence Lifetime Phenomena

The workshop will be co-led by Dr. Brian Bacskai of the MassGeneral
Institute of Neurodegenerative Disease, part of Massachusetts General
Hospital (a Harvard Medical School affiliate), and Dr. Wolfgang
Becker of Becker & Hickl GmbH.

Click or paste into browser for registration WebSite:
http://www.boselec.com/Conferences.htm
or
http://guest.cvent.com/EVENTS/Info/Summary.aspx?e=b2552116-6986-4d57-bd9e-6816c043e8f5



Login Host: 72.93.169.137
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 12 -- From zaluzec-at-microscopy.com Tue Aug 18 12:04:29 2009
11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7IH4SXn004239
11, 12 -- for {microscopy-at-microscopy.com} ; Tue, 18 Aug 2009 12:04:29 -0500
11, 12 -- Mime-Version: 1.0
11, 12 -- Message-Id: {p06240801c6b08f88a50a-at-[206.69.208.22]}
11, 12 -- Date: Tue, 18 Aug 2009 12:04:26 -0500
11, 12 -- To: microscopy-at-microscopy.com
11, 12 -- From: fsp-at-boselec.com (by way of MicroscopyListserver)
11, 12 -- Subject: viaWWW: Workshop on FLIM - Fluorescence Lifetime Imaging
11, 12 -- Microscopy
11, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 19 Aug 2009 03:29:18 -0500
Subject: [Microscopy] scanning speed and EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear community,

Please allow me to group 2 unrelated questions (+ my thanks in advance) in 1 message since this will avoid a second wave of "out of office" replies.

1) Theoretically the scanning speed in SEM should have no effect on EDX analysis, am I right? What about reality? Does the scanning speed influence the EDX analysis in any way?

2) I am trying to determine if a redox reaction occured at the surface of a material. If this reactions occured I should have a thin metallic film at the surface of the material. If I analyze the surface from an angle normal to the surface ("above"), what would be the optimal HT? The film is expected to be thin, so on the one hand it may be advisable to use high HT to increase the signal. However on the other hand a high HT would penetrate deeper, making the part of the signal due to surface material less significant (basically I would analyse the bulk material, not its surface). What would you advise? A HT as low as possible?
I am aware that this method is probably not optimal, but I am not sure if I can do something else. I have no machine to make nice clean cross sections, I fear that the film would detach if I simply break the material with a hammer. The ionic species which are expected to be deposited are Ag and Cu.

Many thanks in advance for your help.

Best regards,

Stephane





==============================Original Headers==============================
10, 22 -- From nizets2-at-yahoo.com Wed Aug 19 03:29:18 2009
10, 22 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7J8THra012634
10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 03:29:18 -0500
10, 22 -- Received: (qmail 20718 invoked by uid 60001); 19 Aug 2009 08:29:17 -0000
10, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250670557; bh=Tpe6lBDcks71pSGbWYhK4lIilkZAtC1SUSSt080aK2I=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=lGmz9LVSqL039/6Ovbjvu5ZVMebgvuyJLfn2VpoWcaPvv9Jg9eCGuHW0JkiZzy/FV6KnNiwRiRy87t9jaBz1Hw6bF1sLBAlnHZ3aeBH4qVsk6WpyvzsiA5Zy1E53p6EUBJl0be8OHKT6gHfO76SyXfI87YZITpXsi0nOebp0Ofw=
10, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 22 -- s=s1024; d=yahoo.com;
10, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
10, 22 -- b=zv1klmTtOlFPNWKMHj1h4e6VZfudtPh9NVnRGcdcshcw455iYJcOdVxPRCImj7tcyp3NCHlZknYAIfzaaHOhWXGPF9HdBEBwBFTpue3kyyJQUKxQnpcMmGqYvsOz2PyhigYVwb5ryyyQXgcZJGrh7UXIHMKiJYe8zMbcgjpq+MY=;
10, 22 -- Message-ID: {363047.19394.qm-at-web110802.mail.gq1.yahoo.com}
10, 22 -- X-YMail-OSG: 5yWh7QYVM1moZugfwNcXqkEYYLGgdzkAaNoA07stC0NhK3x2kC0q1sYx3h4jzogM5EwTVaC6UrF_KHq4jdISbPmn86GENksIDMDjnqGEny9arRxQ1Fs.qF2bIccyisu_MQHUyk34ZjFXE5DIOqTEKUhlk49RqdnsPW.jMZCF94sOYeB1i18tm_5ehyvv318fzvfNv.ZMxUjsRFu62gb08NVLVrB.J097j8495hulTTMmt7g09GaTiDSZdCuquKc4JjH0oqH75TLsXdRAfDk_HiyUcqjgECkmPKWtpkABvhFF8CxrLVtk2rIwFYb9KDMGgpxLnpYd
10, 22 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Wed, 19 Aug 2009 01:29:17 PDT
10, 22 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.1
10, 22 -- Date: Wed, 19 Aug 2009 01:29:17 -0700 (PDT)
10, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
10, 22 -- Subject: scanning speed and EDX
10, 22 -- To: microscopy-at-microscopy.com
10, 22 -- MIME-Version: 1.0
10, 22 -- Content-Type: text/plain; charset=iso-8859-1
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7J8THra012634
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 19 Aug 2009 08:37:42 -0500
Subject: [Microscopy] nanoscopic scale and under

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear community,

After a discussion with my colleagues chemists, we don't agree on one theoretical question and I wanted to submit it to the list and collect your opinions.
Following my colleagues iodine (wether I or I-) would have too large a diameter to be stuffed in nanotubes (in the channels).
I don't understand this point of view, because the atomic scale is well under the nm! I know that ions are envelopped with water, but I don't think one can exclude that the ions can enter nanoscopic structures only based on this reason. The water molecules are not so strongly bound!
Could someone with experience with nanomaterial give his opinion?
Opinions supported by literature would be preferred ;-)

Best regards,

Stephane




==============================Original Headers==============================
6, 20 -- From nizets2-at-yahoo.com Wed Aug 19 08:37:41 2009
6, 20 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7JDbf74008046
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 08:37:41 -0500
6, 20 -- Received: (qmail 25542 invoked by uid 60001); 19 Aug 2009 13:37:41 -0000
6, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250689061; bh=PzwFsNamvYtGsU5qf8YuC7eY9wpVSoVk6Ux47/MWnVU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=r0xutsljEqFpI4Km0+MBBP+EFGMHb2AYyngHSpg6V8HVPKPXAqZ1c/pI9HbWcqLhRcU9awQCdN26Bs1FOizRBCMe5OCLv5Z0c9rbBPxeqZWg9lOluYKwZap67XXYXG426h6WIUhIxcFvF1Dp2dIV2DxTImSosYJJ7wpTw9hsQ64=
6, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
6, 20 -- s=s1024; d=yahoo.com;
6, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
6, 20 -- b=EnKg152iCU1dfKwZ64oj3nJd+oaUZ61pfmyMXKGW9ZrmuljEQnTpX56wFg4hAIvbPkQ4r2R1rn9HJl8WXRpY4b+9oIDkPrddNWFpmsjDQP6G9P9XiFQkc2LxAU4MuXdejpNTmnhHqbW941AgQI28EFsEZd9Xx0cvzTK+5SewW6A=;
6, 20 -- Message-ID: {27368.25233.qm-at-web110801.mail.gq1.yahoo.com}
6, 20 -- X-YMail-OSG: GgB9la4VM1k51BGYPt1oe0PGKqzgGBNFzJLjZxE0RiZFUBryrV_CW65KXYtlbgs9MQtRfP84k096vkxXhHnkflL.nEx5OkZCnYkEOtRROhHVsU0KEkgHTNJ15t16sQfXhhCqC_dwOH60eRZ8J8laOzdH4FCl2NAtNjss.6aR_Dk7ysP2_lmXZs4Qw5s8hfq5PSGve5G7QNM62P8JytaQ.Sc_sEC9YTmFsv7aVqJNuH3a5b4F6sxmuerbDChGPLhpm7sgFYKaJVvgzetNZEqlUXia2l4z1_NGhr2H7.Ns0NXrFIyNpkCbqbQLisLTlbmis2M3eM6u
6, 20 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Wed, 19 Aug 2009 06:37:40 PDT
6, 20 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.1
6, 20 -- Date: Wed, 19 Aug 2009 06:37:40 -0700 (PDT)
6, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
6, 20 -- Subject: nanoscopic scale and under
6, 20 -- To: microscopy-at-microscopy.com
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 19 Aug 2009 08:37:46 -0500
Subject: [Microscopy] nanoscopic scale and under

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear community,

After a discussion with my colleagues chemists, we don't agree on one theoretical question and I wanted to submit it to the list and collect your opinions.
Following my colleagues iodine (wether I or I-) would have too large a diameter to be stuffed in nanotubes (in the channels).
I don't understand this point of view, because the atomic scale is well under the nm! I know that ions are envelopped with water, but I don't think one can exclude that the ions can enter nanoscopic structures only based on this reason. The water molecules are not so strongly bound!
Could someone with experience with nanomaterial give his opinion?
Opinions supported by literature would be preferred ;-)

Best regards,

Stephane

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
5, 20 -- From nizets2-at-yahoo.com Wed Aug 19 08:37:46 2009
5, 20 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7JDbjgo008081
5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 08:37:46 -0500
5, 20 -- Received: (qmail 25575 invoked by uid 60001); 19 Aug 2009 13:37:45 -0000
5, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1250689065; bh=8GjjFstjgRlF3G1zxr6NhXoyrhEfx4YIfHann+NZxIg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=ZbeytHXMKZtHw9l0SxBHVPrJkpgMXdYhrpgaCGGD1230kdlB2XeJTkO1xaLRolFWkZ5gSDxZGBvmHi9Vq9fEKO+1mteFG6G1V4/n/HU/mP6kp3JaToF2wSk+3n3OFd+DcnsnosYxLwiS2kdBGGgymNvgz/meD3S3+UfDiBuwlh4=
5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 20 -- s=s1024; d=yahoo.com;
5, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
5, 20 -- b=FBXBKiOapYOnHdNrpYI3jUKdtAYLkPK17KPGZDPtlY7/B127BCP9sp1zNA78xGCy42fLBpDXhIyWyQllyHbVMOfyPhRQeArCBlMTgxu5j8gWeoseEASNtrU8DqAy5XBzB5W75Dqw5pNktCgrxfXGpFf9WiYm3rqKN+InFH3O62M=;
5, 20 -- Message-ID: {763938.25228.qm-at-web110801.mail.gq1.yahoo.com}
5, 20 -- X-YMail-OSG: 2cy_bdIVM1kV_imxlf4I73J6ZMA3rJIhJpFl4s6BbkMi63fELKbqaRu.u2SSWavVje6KJNSosTret9sFcSI7CuYYlbzDeuYV5skypR1jara_J1335EQxu_dim.3_kXcx9R8.148HFwT_D5yWHgK7LSCejmnSJseiUv0OonIu_veSY.ECjRj4qDoF2Azp3MQInC7OXqMO8LdePSC2h6n5YmB_b9ASRUg3vWMMEUHY06h7Fnr31qtJNiTPK2GW7q9zQlqPdiCndcdUW3oNHAcbP8oeS0_UkeyMYJrWJlQMR9JrEcdHf6YK1tFcuGggGpHc45z27iKw5HJHtRSm_5I0bCY0HALQjGDvyMRB4ZTNm.UDTAMVqaJc9czmoA--
5, 20 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Wed, 19 Aug 2009 06:37:45 PDT
5, 20 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.1
5, 20 -- Date: Wed, 19 Aug 2009 06:37:45 -0700 (PDT)
5, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 20 -- Subject: nanoscopic scale and under
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: tina.williams-at-ars.usda.gov
Date: Wed, 19 Aug 2009 09:55:44 -0500
Subject: [Microscopy] viaWWW: Argon gas cannisters for Alto 2500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tina.williams-at-ars.usda.gov as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tina.williams-at-ars.usda.gov
Name: Tina Williams

Organization: U.S. Dept. of Agriculture

Title-Subject: [Filtered] Argon gas cannisters for Alto 2500

Question: Does anyone know where replacement Argon gas cannisters for
the Alto 2500 cryoprep/sputtering unit can be purchased? It is dry
and high purity. The old cannister was purchased from
Messer,cat.TGS0012, but may have come along with the unit.

Thank you.

Login Host: 199.133.214.91
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Aug 19 09:55:44 2009
7, 11 -- Received: from [78.64.120.74] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7JEtfGI006432
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 09:55:42 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c6b1c2db065a-at-[78.64.120.74]}
7, 11 -- Date: Wed, 19 Aug 2009 09:55:40 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: tina.williams-at-ars.usda.gov (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Argon gas cannisters for Alto 2500
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: klivi-at-jhu.edu
Date: Wed, 19 Aug 2009 12:31:28 -0500
Subject: [Microscopy] TEM open EM position UNC at Charlotte

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am posting this for a friend. Please do not respond to me, but to
Terry Xu txu-at-uncc.edu. Thank You.
Ken


Duke Energy Postdoctoral Fellowship ⎯ Electron Microscopist
Charlotte Research Institute: Center for Optoelectronics and Optical
Communication
The University of North Carolina at Charlotte
The University of North Carolina at Charlotte is establishing a
Transmission Electron
Microscopy (TEM) facility. The facility housed in the Center for
Optoelectronics and Optical
Communication will operate a new JEOL JEM-2100 transmission electron
microscope equipped with a Gatan Orius camera and an Oxford INCA EDS
system, and a variety of sample
preparation tools including precision ion polishing system,
ultramicrotome, etc. The facility is
seeking to hire an electron microscopist to provide high-level
expertise and conduct original
research in the field of electron microscopy. The successful candidate
will be hired through the
Charlotte Research Institute – Duke Energy Postdoctoral Fellowship
Program. Review of
candidates will begin immediately, and will continue until the
position is filled. The candidate is
expected to start the work on September 1, 2009.
Position summary
The role of the electron microscopist is to lead the operation and the
user program of the facility, and conduct original research with
advanced TEM-based techniques. More specifically, the
candidate will be responsible for teaching lab sections of TEM,
supporting and training the users, collaborating with internal and
external investigators to perform research wherever needs for advanced
electron microscopy techniques arise.
Position Requirements
1. A Ph.D. degree in Materials Science, Physics, Chemistry,
Engineering or related areas.
2. Advanced understanding and skills in the acquisition and
interpretation of electron
microscopy data in the physical science.
3. At least three years of operation and maintenance experience in TEM
and associated sample preparation tools. Experience with scanning
electron microscopy, x-ray diffractometry and other materials science
characterization techniques will be plus.
4. A good research publication record.
5. Strong oral and written communication skills.
Application Process
The applicant should submit electronically a current curriculum vitae,
a summary of expertise
and accomplishments and contact information of three references to:
Dr. Terry Xu at
ttxu-at-uncc.edu.



==============================Original Headers==============================
5, 18 -- From klivi-at-jhu.edu Wed Aug 19 12:31:18 2009
5, 18 -- Received: from ipex2.johnshopkins.edu (ipex2.johnshopkins.edu [162.129.8.151])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7JHVHFT025182
5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 12:31:18 -0500
5, 18 -- X-IronPort-AV: E=Sophos;i="4.43,409,1246852800";
5, 18 -- d="scan'208";a="215452429"
5, 18 -- Received: from olin60-959-3185.olin.jhu.edu ([128.220.60.112])
5, 18 -- by ipex2.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 19 Aug 2009 13:31:11 -0400
5, 18 -- Message-Id: {E68E902F-7776-494B-A726-2EF85F4BA6DB-at-jhu.edu}
5, 18 -- From: Ken Livi {klivi-at-jhu.edu}
5, 18 -- To: Microscopy-at-microscopy.com
5, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=yes
5, 18 -- Mime-Version: 1.0 (Apple Message framework v935.3)
5, 18 -- Subject: TEM open EM position UNC at Charlotte
5, 18 -- Date: Wed, 19 Aug 2009 13:31:11 -0400
5, 18 -- X-Mailer: Apple Mail (2.935.3)
5, 18 -- Content-Transfer-Encoding: 8bit
5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7JHVHFT025182
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Wed, 19 Aug 2009 14:04:09 -0500
Subject: [Microscopy] scanning speed and EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stephane,
Since the EDX detector is gathering all the x-rays from the sample, it
doesn't know or care whether the beam is scanning or still or what area the
beam is scanning. You should just be aware that the SEM's scanning pattern
includes a "pause" at the upper left-hand corner of the scanned rectangle
and at the left-hand side of every line, so the x-rays will not be evenly
acquired from the whole area scanned. Some SEMs have a special "Analysis
Mode" that moves the beam evenly on a rectangle to give an even x-ray
distribution over the area.
If you want to analyse a thin metal film on top of a substrate, you would be
advised to lower the Kv and look for the L lines of the metal. Increase the
beam current to increase the signal. Use an accelerating voltage no more
than twice the energy of the metal's L line. If you use a high Kv and try to
get the K lines of your metal, you will get too much x-ray flux from the
substrate and will not be able to detect the x-rays from the thin metal film
on top.
I hope this helps.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: August 19, 2009 1:38 AM
To: maryflet-at-interchange.ubc.ca

Dear community,

Please allow me to group 2 unrelated questions (+ my thanks in advance) in 1
message since this will avoid a second wave of "out of office" replies.

1) Theoretically the scanning speed in SEM should have no effect on EDX
analysis, am I right? What about reality? Does the scanning speed influence
the EDX analysis in any way?

2) I am trying to determine if a redox reaction occured at the surface of a
material. If this reactions occured I should have a thin metallic film at
the surface of the material. If I analyze the surface from an angle normal
to the surface ("above"), what would be the optimal HT? The film is expected
to be thin, so on the one hand it may be advisable to use high HT to
increase the signal. However on the other hand a high HT would penetrate
deeper, making the part of the signal due to surface material less
significant (basically I would analyse the bulk material, not its surface).
What would you advise? A HT as low as possible?
I am aware that this method is probably not optimal, but I am not sure if I
can do something else. I have no machine to make nice clean cross sections,
I fear that the film would detach if I simply break the material with a
hammer. The ionic species which are expected to be deposited are Ag and Cu.

Many thanks in advance for your help.

Best regards,

Stephane





==============================Original Headers==============================
10, 22 -- From nizets2-at-yahoo.com Wed Aug 19 03:29:18 2009
10, 22 -- Received: from web110802.mail.gq1.yahoo.com
(web110802.mail.gq1.yahoo.com [67.195.13.225])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n7J8THra012634
10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 03:29:18
-0500
10, 22 -- Received: (qmail 20718 invoked by uid 60001); 19 Aug 2009 08:29:17
-0000
10, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1250670557; bh=Tpe6lBDcks71pSGbWYhK4lIilkZAtC1SUSSt080aK2I=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
b=lGmz9LVSqL039/6Ovbjvu5ZVMebgvuyJLfn2VpoWcaPvv9Jg9eCGuHW0JkiZzy/FV6KnNiwRiR
y87t9jaBz1Hw6bF1sLBAlnHZ3aeBH4qVsk6WpyvzsiA5Zy1E53p6EUBJl0be8OHKT6gHfO76SyXf
I87YZITpXsi0nOebp0Ofw=
10, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 22 -- s=s1024; d=yahoo.com;
10, 22 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type:Content-Transfer-Encoding;
10, 22 --
b=zv1klmTtOlFPNWKMHj1h4e6VZfudtPh9NVnRGcdcshcw455iYJcOdVxPRCImj7tcyp3NCHlZkn
YAIfzaaHOhWXGPF9HdBEBwBFTpue3kyyJQUKxQnpcMmGqYvsOz2PyhigYVwb5ryyyQXgcZJGrh7U
XIHMKiJYe8zMbcgjpq+MY=;
10, 22 -- Message-ID: {363047.19394.qm-at-web110802.mail.gq1.yahoo.com}
10, 22 -- X-YMail-OSG:
5yWh7QYVM1moZugfwNcXqkEYYLGgdzkAaNoA07stC0NhK3x2kC0q1sYx3h4jzogM5EwTVaC6UrF_
KHq4jdISbPmn86GENksIDMDjnqGEny9arRxQ1Fs.qF2bIccyisu_MQHUyk34ZjFXE5DIOqTEKUhl
k49RqdnsPW.jMZCF94sOYeB1i18tm_5ehyvv318fzvfNv.ZMxUjsRFu62gb08NVLVrB.J097j849
5hulTTMmt7g09GaTiDSZdCuquKc4JjH0oqH75TLsXdRAfDk_HiyUcqjgECkmPKWtpkABvhFF8Cxr
LVtk2rIwFYb9KDMGgpxLnpYd
10, 22 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com
via HTTP; Wed, 19 Aug 2009 01:29:17 PDT
10, 22 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.1
10, 22 -- Date: Wed, 19 Aug 2009 01:29:17 -0700 (PDT)
10, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
10, 22 -- Subject: scanning speed and EDX
10, 22 -- To: microscopy-at-microscopy.com
10, 22 -- MIME-Version: 1.0
10, 22 -- Content-Type: text/plain; charset=iso-8859-1
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n7J8THra012634
==============================End of - Headers==============================



==============================Original Headers==============================
20, 34 -- From maryflet-at-interchange.ubc.ca Wed Aug 19 14:04:09 2009
20, 34 -- Received: from mr7.mail-relay.ubc.ca (mr7.mail-relay.ubc.ca [137.82.45.13])
20, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7JJ48re009202
20, 34 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 14:04:09 -0500
20, 34 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
20, 34 -- by mr7.mail-relay.ubc.ca (Postfix) with ESMTP id 1FF901C291
20, 34 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 12:04:06 -0700 (PDT)
20, 34 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
20, 34 -- by smtp.interchange.ubc.ca
20, 34 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
20, 34 -- with ESMTP id {0KON00D8G0YTXW-at-smtp.interchange.ubc.ca} for
20, 34 -- microscopy-at-microscopy.com; Wed, 19 Aug 2009 12:04:06 -0700 (PDT)
20, 34 -- Date: Wed, 19 Aug 2009 12:03:59 -0700
20, 34 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
20, 34 -- Subject: RE: [Microscopy] scanning speed and EDX
20, 34 -- In-reply-to: {200908190837.n7J8btvb026567-at-ns.microscopy.com}
20, 34 -- To: nizets2-at-yahoo.com
20, 34 -- Cc: microscopy-at-microscopy.com
20, 34 -- Reply-to: maryflet-at-interchange.ubc.ca
20, 34 -- Message-id: {0KON00D8H0YTXW-at-smtp.interchange.ubc.ca}
20, 34 -- Organization: Materials Eng.
20, 34 -- MIME-version: 1.0
20, 34 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 34 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
20, 34 -- Content-type: text/plain; charset=iso-8859-1
20, 34 -- Thread-index: AcogqFlntZwVTYbTToSOHW9/8omIDwAViZvA
20, 34 -- X-UBC-Scanned: Sophos PureMessage 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.8.19.185417
20, 34 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
20, 34 -- X-PerlMx-Spam: Probability=8%, Report=
20, 34 -- BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __USER_AGENT_MS_GENERIC 0
20, 34 -- X-Spam-Level:
20, 34 -- X-Spam-Flag: No
20, 34 -- Content-Transfer-Encoding: 8bit
20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7JJ48re009202
==============================End of - Headers==============================




From: twigg-at-estd.nrl.navy.mil
Date: Wed, 19 Aug 2009 15:06:10 -0500
Subject: [Microscopy] viaWWW: Buying Dimpled Si Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both twigg-at-estd.nrl.navy.mil as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: Naval Research Laboratory

Title-Subject: [Filtered] Buying Dimpled Si Samples

Question: A colleague of mine would like to purchase what would
essentially be 3 mm dimpled plan-view Si TEM samples to use as a
support for a spectroscopy experiment. The thickness of the dimpled
sample in the center would need to be 10 microns or less--that is,
where the Si is thin enough to have a reddish appearance under
optical illumination. Is there anybody out there who sells such a
thing?

Thanks,

Mark

Login Host: 132.250.22.5
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Aug 19 15:06:10 2009
8, 11 -- Received: from [78.64.120.74] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7JK68B4024980
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 15:06:09 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6b20b911112-at-[78.64.120.74]}
8, 11 -- Date: Wed, 19 Aug 2009 15:06:06 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Buying Dimpled Si Samples
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Wed, 19 Aug 2009 21:38:49 -0500
Subject: [Microscopy] Advanced PFM School - ORNL September 15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

We are delighted to bring to our attention the Advanced Piezoresponse Force Microscopy School, to be held in conjunction with the User meeting of the Center for Nanophase Materials Sciences on September 15, 2009. The information on the registration and venue is available at http://www.cnms.ornl.gov/. This workshop is 6th in the series of PFM workshops held in the last 2 years at several locations worldwide, including ORNL, EPFL (Switzerland), U. Aveiro (Portugal), and NIMS (Japan).

The workshop on Advanced PFM aims to provide in-depth description and recent advances in Piezoresponse Force Microscopy, specifically highlighting the recent technical advances in PFM, including vector PFM, high-frequency PFM, band-excitation and DART imaging, switching spectroscopy PFM and imaging and polarization switching in liquids and vacuum that are available as a part of CNMS user program. A demo session illustrating the applications of band excitation PFM and SS-PFM will be included. The program will also include tutorial lectures on thermomechanical imaging, PFM coupled to electrical transport measurements, and multivariate statistics analysis of PFM data.

Please contact Sergei Kalinin (sergei2-at-ornl.gov) and Art Baddorf (baddorfap-at-ornl.gov) with any questions related to venue and program for the school.

Yours

Sergei V. Kalinin
Art P. Baddorf

==============================Original Headers==============================
6, 35 -- From sergei2-at-ornl.gov Wed Aug 19 21:38:49 2009
6, 35 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119])
6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7K2cmaD017571
6, 35 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2009 21:38:49 -0500
6, 35 -- Received: from emroute1.ornl.gov ([127.0.0.1])
6, 35 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
6, 35 -- with ESMTP id {0KON00ANEM0OZB-at-emroute1.ornl.gov} for
6, 35 -- microscopy-at-microscopy.com; Wed, 19 Aug 2009 22:38:48 -0400 (EDT)
6, 35 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov
6, 35 -- (PMDF V6.4 #31561) id {0KON00L01M0NB4-at-emroute1.ornl.gov} for
6, 35 -- microscopy-at-microscopy.com; Wed, 19 Aug 2009 22:38:48 -0400 (EDT)
6, 35 -- Received: from exchhub1.ornl.gov (exchhub1.ornl.gov [160.91.2.111])
6, 35 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
6, 35 -- with ESMTPS id {0KON008D6M0NXF-at-emroute1.ornl.gov} for
6, 35 -- microscopy-at-microscopy.com; Wed, 19 Aug 2009 22:38:47 -0400 (EDT)
6, 35 -- Received: from EXCHMB.ornl.gov ([160.91.2.201])
6, 35 -- by exchhub1.ornl.gov ([160.91.2.111]) with mapi; Wed,
6, 35 -- 19 Aug 2009 22:38:47 -0400
6, 35 -- Date: Wed, 19 Aug 2009 22:38:46 -0400
6, 35 -- From: "Kalinin, Sergei V." {sergei2-at-ornl.gov}
6, 35 -- Subject: Advanced PFM School - ORNL September 15
6, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
6, 35 -- Message-id: {37187374E1157F409035F4EB48DE96BD03FF7CB8D342-at-EXCHMB.ornl.gov}
6, 35 -- MIME-version: 1.0
6, 35 -- Content-type: text/plain; charset=us-ascii
6, 35 -- Content-language: en-US
6, 35 -- Importance: high
6, 35 -- Thread-Topic: Advanced PFM School - ORNL September 15
6, 35 -- Thread-Index: AQHKIT9XWRVqTm9gZEWHLTEmPbVo6w==
6, 35 -- Accept-Language: en-US
6, 35 -- acceptlanguage: en-US
6, 35 -- X-MS-Has-Attach:
6, 35 -- X-MS-TNEF-Correlator:
6, 35 -- Content-Transfer-Encoding: 8bit
6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7K2cmaD017571
==============================End of - Headers==============================




From: hubner-at-m6.mech.pk.edu.pl
Date: Thu, 20 Aug 2009 02:29:18 -0500
Subject: [Microscopy] EWGAE - European Working Group on Acoustic Emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

29th EUROPEAN CONFERENCE ON ACOUSTIC EMISSION TESTING

will be held in VIENNA, AUSTRIA
on September 8÷10th, 2010

Organizer: TÜV Austria Services GmbH

http://www.ewgae.eu/page.html

best regards

Krzysztof Hübner

Cracow University of Technology
Institute of Production Engineering
Laboratory of Applied Research
al. Jana Paw³a II 37
31-864 Kraków
Poland



==============================Original Headers==============================
10, 31 -- From hubner-at-m6.mech.pk.edu.pl Thu Aug 20 02:29:16 2009
10, 31 -- Received: from m6.mech.pk.edu.pl (m6.mech.pk.edu.pl [149.156.153.154])
10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7K7TGm5006635
10, 31 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 02:29:16 -0500
10, 31 -- Received: from localhost (unknown [127.0.0.1])
10, 31 -- by m6.mech.pk.edu.pl (Postfix) with ESMTP id 4AC363FF
10, 31 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 09:57:58 +0200 (MEST)
10, 31 -- Received: from m6.mech.pk.edu.pl ([127.0.0.1])
10, 31 -- by localhost (m6 [127.0.0.1]) (amavisd-new, port 10024) with ESMTP
10, 31 -- id 14157-04 for {microscopy-at-microscopy.com} ;
10, 31 -- Thu, 20 Aug 2009 09:57:27 +0200 (MEST)
10, 31 -- Received: from Krzysiek (unknown [149.156.153.184])
10, 31 -- by m6.mech.pk.edu.pl (Postfix) with SMTP id 2D604327
10, 31 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 09:57:27 +0200 (MEST)
10, 31 -- Message-ID: {B06563F446CB4D08A8413E082412DCD6-at-Krzysiek}
10, 31 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-m6.mech.pk.edu.pl}
10, 31 -- To: "Microscopy list" {microscopy-at-microscopy.com}
10, 31 -- Subject: EWGAE - European Working Group on Acoustic Emission
10, 31 -- Date: Thu, 20 Aug 2009 09:28:42 +0200
10, 31 -- Organization: =?iso-8859-2?Q?Politechnika_Krakowska_Wydzia=B3_Mechaniczny?=
10, 31 -- MIME-Version: 1.0
10, 31 -- Content-Type: text/plain;
10, 31 -- format=flowed;
10, 31 -- charset="iso-8859-2";
10, 31 -- reply-type=original
10, 31 -- Content-Transfer-Encoding: 8bit
10, 31 -- X-Priority: 3
10, 31 -- X-MSMail-Priority: Normal
10, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843
10, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
10, 31 -- X-Virus-Scanned: by amavisd-new at m6.mech.pk.edu.pl
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Thu, 20 Aug 2009 10:03:46 -0500
Subject: [Microscopy] Cryo-EM position posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have noticed the following position posting at NIH. If interested check
out the site below. I have pasted only part of the description. It is not
necessary that you are familiar with every technique listed but the cryo-EM
seems to be a must.

Important! The end date for applying is 8/21 and the application takes
hours to fill in on USA JOBS. I believe that you must be a citizen of the
USA.

If you are not employed by the government the starting salary is the lowest
posted.
==

Biologist - National Cancer Institute - CR -DE
{http://jobview.usajobs.gov/getjob.asp?JobID=82810211&aid=38802312-1789&WT.m
c_n=125}

GS-0401-12/12

$73,100-$95,026

Health And Human Services/National Institutes of Health

Bethesda, MD

08/21/2009

Conduct experimental studies or part of a study by performing
complex procedures and techniques which may include, but are not
limited to, the following: isolation and purification of monoclonal
antibodies, radioimmunoassay, protein analysis, protein refolding
and DNA replication, protein purification, radioactive labeling of
proteins in vitro and vivo, DNA extraction and purification, high
pressure liquid chromatography, column chromatography, mass
spectrophotometry, peptide synthesis, immunoprecipitation, enzyme
linked immunoabsorbant assay, cell/tissue culture, and thorough in-
depth knowledge of preparation of biological material for
examination using CRYO-ELECTRON MICROSCOPY.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E ­ Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not
represent the NIH.



==============================Original Headers==============================
14, 31 -- From connellyps-at-nhlbi.nih.gov Thu Aug 20 10:03:46 2009
14, 31 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113])
14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KF3j8q003043
14, 31 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 10:03:46 -0500
14, 31 -- X-IronPortListener: Outbound_SMTP
14, 31 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117])
14, 31 -- by nihxway5out.hub.nih.gov with ESMTP; 20 Aug 2009 11:03:45 -0400
14, 31 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
14, 31 -- Thu, 20 Aug 2009 11:03:44 -0400
14, 31 -- Received: from NIHHTRC.nih.gov (156.40.71.74) by NIHHT01.nih.gov
14, 31 -- (156.40.71.20) with Microsoft SMTP Server (TLS) id 8.1.358.0; Thu, 20 Aug
14, 31 -- 2009 11:03:44 -0400
14, 31 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHTRC.nih.gov
14, 31 -- ([156.40.71.74]) with mapi; Thu, 20 Aug 2009 11:03:44 -0400
14, 31 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
14, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
14, 31 -- Date: Thu, 20 Aug 2009 11:00:35 -0400
14, 31 -- Subject: Cryo-EM position posting
14, 31 -- Thread-Topic: Cryo-EM position posting
14, 31 -- Thread-Index: AcohpvhyNtpHpo2aEd6ILwANk2Yv1A==
14, 31 -- Message-ID: {C6B2DD53.3D68%connellyps-at-nhlbi.nih.gov}
14, 31 -- Accept-Language: en-US
14, 31 -- Content-Language: en
14, 31 -- X-MS-Has-Attach:
14, 31 -- X-MS-TNEF-Correlator:
14, 31 -- acceptlanguage: en-US
14, 31 -- Content-Type: text/plain; charset="iso-8859-1"
14, 31 -- MIME-Version: 1.0
14, 31 -- X-OriginalArrivalTime: 20 Aug 2009 15:03:44.0872 (UTC) FILETIME=[699E5680:01CA21A7]
14, 31 -- Content-Transfer-Encoding: 8bit
14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7KF3j8q003043
==============================End of - Headers==============================




From: William.H.Roberts-at-USA.dupont.com
Date: Thu, 20 Aug 2009 10:47:37 -0500
Subject: [Microscopy] LM user needs help for Polaroid DMC-1e software issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Does anyone know if updated software is available to run the Polaroid
DMC-1e camera using a computer with Windows XP operating system? The
original computer was a Win98 version, but it died. Polaroid's customer
support telephone message says that they no longer support the system. Do

I need a new camera (I know that I do, just need confirmation.)?

TIA Bill Roberts

This communication is for use by the intended recipient and contains
information that may be Privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender by
return e-mail and delete this e-mail from your system. Unless explicitly
and conspicuously designated as "E-Contract Intended", this e-mail does
not constitute a contract offer, a contract amendment, or an acceptance
of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html


==============================Original Headers==============================
8, 28 -- From William.H.Roberts-at-USA.dupont.com Thu Aug 20 10:47:37 2009
8, 28 -- Received: from mail203.messagelabs.com (mail203.messagelabs.com [216.82.254.243])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7KFlakm018375
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 10:47:37 -0500
8, 28 -- X-VirusChecked: Checked
8, 28 -- X-Env-Sender: William.H.Roberts-at-USA.dupont.com
8, 28 -- X-Msg-Ref: server-12.tower-203.messagelabs.com!1250783255!47774296!1
8, 28 -- X-StarScan-Version: 6.1.3; banners=-,-,-
8, 28 -- X-Originating-IP: [52.129.16.69]
8, 28 -- Received: (qmail 16278 invoked from network); 20 Aug 2009 15:47:35 -0000
8, 28 -- Received: from unknown (HELO demhub21.lvs.dupont.com) (52.129.16.69)
8, 28 -- by server-12.tower-203.messagelabs.com with SMTP; 20 Aug 2009 15:47:35 -0000
8, 28 -- Received: from unknown (HELO demhub1.lvs.dupont.com) ([52.99.10.45])
8, 28 -- by demhub21a.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:35 -0400
8, 28 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com}
8, 28 -- Received: from cdcln05.lvs.dupont.com ([52.99.26.10])
8, 28 -- by demhub1.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:34 -0400
8, 28 -- To: Microscopy-at-microscopy.com
8, 28 -- Subject: LM user needs help for Polaroid DMC-1e software issue
8, 28 -- MIME-Version: 1.0
8, 28 -- X-Mailer: Lotus Notes Release 7.0.1 CCH2 May 01, 2006
8, 28 -- Message-ID: {OF77C1DB99.81A8CB13-ON85257618.00560DA5-85257618.0056BF74-at-CDCLN05.LVS.DUPONT.COM}
8, 28 -- Date: Thu, 20 Aug 2009 11:47:31 -0400
8, 28 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release 8.5 HF460|May 15, 2009) at
8, 28 -- 08/20/2009 11:47:34 AM,
8, 28 -- Serialize complete at 08/20/2009 11:47:34 AM
8, 28 -- Content-Transfer-Encoding: 7bit
8, 28 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: hindc-at-baxter.com
Date: Thu, 20 Aug 2009 11:09:14 -0500
Subject: [Microscopy] viaWWW: FEI Quanta 250 and Inspect S50 SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both hindc-at-baxter.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: hindc-at-baxter.com
Name: Cameron

Organization: Baxter

Title-Subject: [Filtered] FEI Quanta 250 and Inspect S50 SEMs

Question: Hi Listservers,

I'm out of touch with the price of SEMs these days; anyone out there
with an idea of a ballpark figure for the above SEMs?
Local FEI people don't give out figures but before they come and talk
to me I'd like to have an idea whether I'm in for a shock or a
(relatively) pleasant surprise.

regards,

CH

Login Host: 167.83.9.20
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Aug 20 11:09:13 2009
9, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KG9A2x000757
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 11:09:12 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c6b32378a487-at-[78.64.120.92]}
9, 11 -- Date: Thu, 20 Aug 2009 11:00:09 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: hindc-at-baxter.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: FEI Quanta 250 and Inspect S50 SEMs
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gas19-at-chrysler.com
Date: 08/20/2009 11:53AM
Subject: [Microscopy] LM user needs help for Polaroid DMC-1e software issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We had the same issue a few years ago. We had all of our cameras on
Windows 2000 computers. One of the computers died, so we tried it on a
Windows XP computer, and could not get it to work. Luckily we had a slow
transition from Windows 2000 to Windows XP, and were able to upgrade all of
our cameras without loosing function for too long.

I think you need to buy a new camera.

Gerald Shulke
Materials Engineering Specialist
Materials Characterization Labs
Chrysler Group LLC



-----William.H.Roberts-at-USA.dupont.com wrote: -----


To: gas19-at-chrysler.com
X-from: William.H.Roberts-at-USA.dupont.com




----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear Listers,

Does anyone know if updated software is available to run the Polaroid
DMC-1e camera using a computer with Windows XP operating system? The
original computer was a Win98 version, but it died. Polaroid's customer
support telephone message says that they no longer support the system. Do

I need a new camera (I know that I do, just need confirmation.)?

TIA Bill Roberts

This communication is for use by the intended recipient and contains
information that may be Privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender by
return e-mail and delete this e-mail from your system. Unless explicitly
and conspicuously designated as "E-Contract Intended", this e-mail does
not constitute a contract offer, a contract amendment, or an acceptance
of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html


==============================Original
Headers==============================
8, 28 -- From William.H.Roberts-at-USA.dupont.com Thu Aug 20 10:47:37 2009
8, 28 -- Received: from mail203.messagelabs.com (mail203.messagelabs.com
[216.82.254.243])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n7KFlakm018375
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 10:47:37
-0500
8, 28 -- X-VirusChecked: Checked
8, 28 -- X-Env-Sender: William.H.Roberts-at-USA.dupont.com
8, 28 -- X-Msg-Ref:
server-12.tower-203.messagelabs.com!1250783255!47774296!1
8, 28 -- X-StarScan-Version: 6.1.3; banners=-,-,-
8, 28 -- X-Originating-IP: [52.129.16.69]
8, 28 -- Received: (qmail 16278 invoked from network); 20 Aug 2009 15:47:35
-0000
8, 28 -- Received: from unknown (HELO demhub21.lvs.dupont.com)
(52.129.16.69)
8, 28 -- by server-12.tower-203.messagelabs.com with SMTP; 20 Aug 2009
15:47:35 -0000
8, 28 -- Received: from unknown (HELO demhub1.lvs.dupont.com)
([52.99.10.45])
8, 28 -- by demhub21a.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:35
-0400
8, 28 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com}
8, 28 -- Received: from cdcln05.lvs.dupont.com ([52.99.26.10])
8, 28 -- by demhub1.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:34 -0400
8, 28 -- To: Microscopy-at-microscopy.com
8, 28 -- Subject: LM user needs help for Polaroid DMC-1e software issue
8, 28 -- MIME-Version: 1.0
8, 28 -- X-Mailer: Lotus Notes Release 7.0.1 CCH2 May 01, 2006
8, 28 -- Message-ID: {OF77C1DB99.81A8CB13-ON85257618.00560DA5-85257618.0056
BF74-at-CDCLN05.LVS.DUPONT.COM}
8, 28 -- Date: Thu, 20 Aug 2009 11:47:31 -0400
8, 28 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release
8.5 HF460|May 15, 2009) at
8, 28 -- 08/20/2009 11:47:34 AM,
8, 28 -- Serialize complete at 08/20/2009 11:47:34 AM
8, 28 -- Content-Transfer-Encoding: 7bit
8, 28 -- Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 28 -- From gas19-at-chrysler.com Thu Aug 20 11:35:40 2009
25, 28 -- Received: from shbmap02.extra.chrysler.com (shbmap02.out.extra.chrysler.com [129.9.168.37])
25, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KGZdMT015875
25, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 11:35:39 -0500
25, 28 -- Received: from shbmap04.shdc.chrysler.com (unknown [53.28.160.92])
25, 28 -- by shbmap02.extra.chrysler.com (Symantec Mail Security) with ESMTP id 860CC4E4003
25, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 12:35:39 -0400 (EDT)
25, 28 -- X-AuditID: 8109a824-a99d6bb00000726d-40-4a8d7b5b9ad1
25, 28 -- Received: from sodddg01.wk.dcx.com (odmcp057-vip3.oddc.chrysler.com [53.231.74.218])
25, 28 -- by shbmap04.shdc.chrysler.com (Symantec Brightmail Gateway) with SMTP id 34.C5.03866.B5B7D8A4; Thu, 20 Aug 2009 12:35:39 -0400 (EDT)
25, 28 -- Importance: Normal
25, 28 -- X-Priority: 3 (Normal)
25, 28 -- In-Reply-To: {200908201553.n7KFr5xM028999-at-ns.microscopy.com}
25, 28 -- References: {200908201553.n7KFr5xM028999-at-ns.microscopy.com}
25, 28 -- Subject: Re: [Microscopy] LM user needs help for Polaroid DMC-1e software issue
25, 28 -- MIME-Version: 1.0
25, 28 -- From: gas19-at-chrysler.com
25, 28 -- To: microscopy-at-microscopy.com
25, 28 -- X-MIMETrack: MIME-CD by Notes Server on sctcdm03.wk.dcx.com/Server/Prod/DCX(Release
25, 28 -- 8.0.2HF887 | March 23, 2009) at 08/20/2009 12:36:44,
25, 28 -- MIME-CD complete at 08/20/2009 12:36:44,
25, 28 -- Serialize by Router on sodddg01.wk.dcx.com/Server/Prod/DCX(Release
25, 28 -- 7.0.3HF258 | February 19, 2008) at 08/20/2009 12:35:39 PM
25, 28 -- Message-ID: {OF3042CC7A.DD57B05E-ON85257618.005B4127-85257618.005B412C-at-wkamerica.notes.chrysler.com}
25, 28 -- Date: Thu, 20 Aug 2009 12:36:44 -0400
25, 28 -- X-Mailer: Lotus Domino Web Server Release 8.0.2HF887 March 23, 2009
25, 28 -- Content-type: text/plain; charset=US-ASCII
25, 28 -- X-Brightmail-Tracker: AAAAAA==
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Thu, 20 Aug 2009 11:44:29 -0500
Subject: [Microscopy] TEM block labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KGiTcr030692
for {MicroscopyListserverArchive-at-microscopy.com} ; Thu, 20 Aug 2009 11:44:29 -0500
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n7KGiTUM030689;
Thu, 20 Aug 2009 11:44:29 -0500


Several years ago I posted a message about my system for producing labels
for TEM blocks. It consists of a few Word documents which are templates for
labels. By using Search and Replace in your word processor, you can make
labels quickly and save lots of keystrokes. I made these templates
available, and many people requested them. But I received no feedback at
all, and I am just curious if anyone found them helpful, or if their was
confusion about how to use them. If anyone would like to have copies of the
templates, I will be happy to send them to you.

Ralph Common
Michigan State University


==============================Original Headers==============================
4, 31 -- From rcommon-at-msu.edu Thu Aug 20 11:44:29 2009
4, 31 -- Received: from sys50.mail.msu.edu (sys50.mail.msu.edu [35.9.75.230])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KGiTIc030686
4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 11:44:29 -0500
4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=msu.edu;
4, 31 -- s=mail; h=From:To:Subject:Date:Message-ID:MIME-Version:
4, 31 -- Content-Type:Content-Transfer-Encoding; bh=Fq3ahMuQr4kGwe5z5rWrV
4, 31 -- gG23PfDAycnkOoMUawvhp0=; b=Ehu7Cnxqbu0GzyEMVoAkafqFdifNxGDBgD91L
4, 31 -- XxmlS9Pf2HT+aw3OJLhm0E91ICcXE/5cjZO3Hfzb5ZHwZJ+aWFghX378ag7+ls1x
4, 31 -- 1M3N+RBJ5ViFTdXgBsVBx1kk6IL1ExA10EZsfjPbS7EvFTNmB8ED9lKpz6rQU0Wc
4, 31 -- uT/0k8=
4, 31 -- Received: from [35.9.122.125] (helo=emlab)
4, 31 -- by sys50.mail.msu.edu with esmtpsa (Exim 4.69 #1)
4, 31 -- (TLSv1:RC4-MD5:128)
4, 31 -- id 1MeAkX-0001MD-Ak
4, 31 -- for microscopy-at-microscopy.com; Thu, 20 Aug 2009 12:44:29 -0400
4, 31 -- From: "Ralph Common" {rcommon-at-msu.edu}
4, 31 -- To: {microscopy-at-microscopy.com}
4, 31 -- Subject: TEM block labeling
4, 31 -- Date: Thu, 20 Aug 2009 12:43:21 -0400
4, 31 -- Message-ID: {004001ca21b5$54cdefa0$7d7a0923-at-msu.edu}
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- charset="iso-8859-1"
4, 31 -- Content-Transfer-Encoding: 7bit
4, 31 -- X-Priority: 3 (Normal)
4, 31 -- X-MSMail-Priority: Normal
4, 31 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
4, 31 -- Importance: Normal
4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1983
4, 31 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: nestor-at-microscopy.com
Date: Thu, 20 Aug 2009 12:04:35 -0500
Subject: [Microscopy] Better Job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Now you are getting a 100% verifiable degree in just 4-5 weeks, with the help of your work experience.
You can get Bachelors, Masters or Doctorate degree in a few weeks time.

Call us right now
1.305.460.5721

Drop us your msg, with your full name and contact number so we can call you back.



==============================Original Headers==============================
5, 18 -- From spitz9-at-marina-4r8rw3ho Thu Aug 20 12:04:31 2009
5, 18 -- Received: from marina-4r8rw3ho (f049091225.adsl.alicedsl.de [78.49.91.225])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KH4SYt012889;
5, 18 -- Thu, 20 Aug 2009 12:04:30 -0500
5, 18 -- Received: from 78.49.91.225 by mail.renfroindia.com; Thu, 20 Aug 2009 18:04:29 +0100
5, 18 -- Message-ID: {01ca21c0$a93b8c90$e15b314e-at-spitz9}
5, 18 -- From: "Debera M. Hannah" {nestor-at-microscopy.com}
5, 18 -- To: {nestor-at-microscopy.com}
5, 18 -- Subject: Better Job
5, 18 -- Date: Thu, 20 Aug 2009 18:04:29 +0100
5, 18 -- MIME-Version: 1.0
5, 18 -- Content-Type: text/plain;
5, 18 -- charset="iso-8859-1"
5, 18 -- Content-Transfer-Encoding: 7bit
5, 18 -- X-Priority: 3
5, 18 -- X-MSMail-Priority: Normal
5, 18 -- X-Mailer: Microsoft Outlook Express 4.72.3155.0
5, 18 -- X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3155.0
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Thu, 20 Aug 2009 13:08:00 -0500
Subject: [Microscopy] Choosing ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

We are going to by a new ultramicrotome (no cryo work), and there are
two of them on the market: from Leika (UC7) and from RMS (PT-XL). While
both microtomes looks similar, the price tag is quite different. Could
you please advise me on pluses and minuses of these ultramicrotomes.
Responds off-list are welcome.

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
8, 23 -- From DusevichV-at-umkc.edu Thu Aug 20 13:07:59 2009
8, 23 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KI7xT6028960
8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 13:07:59 -0500
8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 23 -- Thu, 20 Aug 2009 13:07:58 -0500
8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 23 -- Content-class: urn:content-classes:message
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: text/plain;
8, 23 -- charset="us-ascii"
8, 23 -- Subject: Choosing ultramicrotome
8, 23 -- Date: Thu, 20 Aug 2009 13:07:58 -0500
8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB8A7-at-KC-MSX1.kc.umkc.edu}
8, 23 -- X-MS-Has-Attach:
8, 23 -- X-MS-TNEF-Correlator:
8, 23 -- Thread-Topic: Choosing ultramicrotome
8, 23 -- Thread-Index: AcohwSYfd8/M9RF8RaWOX6VPRKkKsw==
8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 23 -- To: {Microscopy-at-microscopy.com}
8, 23 -- X-OriginalArrivalTime: 20 Aug 2009 18:07:59.0030 (UTC) FILETIME=[2668FD60:01CA21C1]
8, 23 -- Content-Transfer-Encoding: 8bit
8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7KI7xT6028960
==============================End of - Headers==============================




From: kusum45-at-yahoo.com
Date: Thu, 20 Aug 2009 15:25:07 -0500
Subject: [Microscopy] viaWWW: JEOL 100CX TEM - Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kusum45-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kusum45-at-yahoo.com
Name: Vin Berry

Organization: Department of Chemical Engineering, West Virginia
University, Morgantown, WV

Title-Subject: [Filtered] JEOL 100CX TEM - Available

Question: We have a JEOL 100CX TEM/STEM available immediately for a
reasonable price. The microscope is in excellent working condition.
It has been in service contract throughout its life till Jan. 2009.
We are getting a brand new microscope so we need the room to install
the new one. So, we want to get rid of it right away. Please send me
an email or call me, preferably my cell to talk about the details.

Vin Berry, Ph.d.
Dept. Chem. Engineering,
West Virginia university,
Morgantown, WV 26505

Home: 304-428-6961
Cell: 304-615-1244
email: kusum45-at-yahoo.com


Login Host: 98.210.154.8
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Thu Aug 20 15:25:07 2009
9, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KKP6A8014487
9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 15:25:07 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240803c6b35edd107f-at-[78.64.120.92]}
9, 11 -- Date: Thu, 20 Aug 2009 15:13:44 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: kusum45-at-yahoo.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: JEOL 100CX TEM - Available
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nestor-at-microscopy.com
Date: Thu, 20 Aug 2009 16:20:36 -0500
Subject: [Microscopy] Get more money for less work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Now you are getting a 100% verifiable degree in just 4-5 weeks, with the help of your work experience.
You can get Bachelors, Masters or Doctorate degree in a few weeks time.

Call us right now
1-305.460.5721

Leave your msg, with your full name and number and we will get back to you shortly.


==============================Original Headers==============================
4, 20 -- From harriettlnc75-at-packo-fn7aim8yd Thu Aug 20 16:20:33 2009
4, 20 -- Received: from packo-fn7aim8yd (dynamic-78-30-175-148.adsl.eunet.rs [78.30.175.148])
4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KLKTBI029832;
4, 20 -- Thu, 20 Aug 2009 16:20:31 -0500
4, 20 -- Message-ID: {01ca21e4$6e2a5a90$94af1e4e-at-harriettlnc75}
4, 20 -- From: "Raquel G. Hedstrom" {nestor-at-microscopy.com}
4, 20 -- To: {nestor-at-microscopy.com}
4, 20 -- Subject: Get more money for less work
4, 20 -- Date: Thu, 20 Aug 2009 22:20:31 +0100
4, 20 -- MIME-Version: 1.0
4, 20 -- Content-Type: text/plain;
4, 20 -- format=flowed;
4, 20 -- charset="iso-8859-2";
4, 20 -- reply-type=original
4, 20 -- Content-Transfer-Encoding: 7bit
4, 20 -- X-Priority: 3
4, 20 -- X-MSMail-Priority: Normal
4, 20 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2905
4, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2905
4, 20 -- X-Spam: Not detected
==============================End of - Headers==============================




From: rbeavers-at-mail.smu.edu
Date: Thu, 20 Aug 2009 16:34:22 -0500
Subject: [Microscopy] Carbon error in EDS data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List,

I have gathered some weight percent compositions of mineral grains in thin sections using EDS and now see error in calculating oxides which I think is due to the inclusion of C in the data set. The C (epoxy mounting material?) should not be part of the mineral composition although I do see a small peak giving me around 10 wt%. If I remove the carbon and redistribute that to the other elements in the composition and convert to oxides my totals are coming out high in the 110 to 117 percent range for elements present.

Element
Wt%
At%
CK
09.26
16.16
OK
34.96
45.79
MgK
09.54
08.22
AlK
02.30
01.78
SiK
25.74
19.21
CaK
13.52
07.07
FeK
04.70
01.76
Matrix
Correction
ZAF


Any thoughts?

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
8, 28 -- From rbeavers-at-mail.smu.edu Thu Aug 20 16:34:21 2009
8, 28 -- Received: from smtap1.systems.smu.edu (smtap1.systems.smu.edu [129.119.65.148])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7KLYL9k011669
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 16:34:21 -0500
8, 28 -- X-IronPort-Anti-Spam-Filtered: true
8, 28 -- X-IronPort-Anti-Spam-Result: ApoEAPBdjUqBd0GE/2dsb2JhbADHawEJiAOITIJMgUwFii0
8, 28 -- Received: from sxht1p1.systems.smu.edu ([129.119.65.132])
8, 28 -- by smtah1.systems.smu.edu with ESMTP/TLS/RC4-MD5; 20 Aug 2009 16:34:21 -0500
8, 28 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
8, 28 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Thu, 20 Aug 2009
8, 28 -- 16:34:21 -0500
8, 28 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
8, 28 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
8, 28 -- "PROBEUSERS-at-LISTS.UMN.EDU" {PROBEUSERS-at-LISTS.UMN.EDU}
8, 28 -- Date: Thu, 20 Aug 2009 16:34:20 -0500
8, 28 -- Subject: Carbon error in EDS data
8, 28 -- Thread-Topic: Carbon error in EDS data
8, 28 -- Thread-Index: Acoh3fqDmnYKWWdLSMmuL9IuKo8E0Q==
8, 28 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB64338301DE-at-SXMBXC.systems.smu.edu}
8, 28 -- Accept-Language: en-US
8, 28 -- Content-Language: en-US
8, 28 -- X-MS-Has-Attach:
8, 28 -- X-MS-TNEF-Correlator:
8, 28 -- acceptlanguage: en-US
8, 28 -- Content-Type: text/plain; charset="iso-8859-1"
8, 28 -- MIME-Version: 1.0
8, 28 -- Content-Transfer-Encoding: 8bit
8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7KLYL9k011669
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 21 Aug 2009 00:04:20 -0500
Subject: [Microscopy] Administrivia: SPAM Leaking through

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues...

You might notice a couple of spam postings leaked through the filter.
I'm working on closing the hole. The problem is not a virus,
but rather address obscuring, i.e. forging senders/receivers address.


Of course this happens when I'm out of the country, so debugging
isn't easy as I don't have all details I need here.

My apologies.

Cheers,

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
7, 17 -- From zaluzec-at-aaem.amc.anl.gov Fri Aug 21 00:04:19 2009
7, 17 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7L54JCP005902
7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 00:04:19 -0500
7, 17 -- Received: from host-78-64-120-92.homerun.telia.com (msdvpn072.msd.anl.gov [130.202.238.72])
7, 17 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id n7L54I8c002895;
7, 17 -- Fri, 21 Aug 2009 00:04:18 -0500
7, 17 -- Message-Id: {4112DE18-04AA-490B-9C11-0DDAE7E00664-at-aaem.amc.anl.gov}
7, 17 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
7, 17 -- To: microscopy-at-microscopy.com
7, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
7, 17 -- Content-Transfer-Encoding: 7bit
7, 17 -- Mime-Version: 1.0 (Apple Message framework v935.3)
7, 17 -- Subject: Administrivia: SPAM Leaking through
7, 17 -- Date: Fri, 21 Aug 2009 00:04:17 -0500
7, 17 -- Cc: Nestor Zaluzec {zaluzec-at-aaem.amc.anl.gov}
7, 17 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: jeff-at-tss-consulting.com
Date: Fri, 21 Aug 2009 00:10:04 -0500
Subject: [Microscopy] viaWWW: Research Assistant position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jeff-at-tss-consulting.com as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jeff-at-tss-consulting.com
Name: Jeff West

Organization: TSS Consulting Ltd

Title-Subject: [Filtered] Research Assistant position

Question: I am searching for an MS in Material
Science With XRD, AFM, EPD etc. who will provide
support to a Sr research Scientist investigating
the development of Photovoltaics. The ideal
candidate will be a recent graduate who's thesis
work was in semiconductor materials and/or (b)
x-ray diffraction analysis, but those arenít
requirements. This is a privately held reseach
company with whom I have this exclusive
assignemnt.
Please forward your resume and contact
information, a good time for me to call and your
availability for this regular employee position
(this is not a contract assignment).
jeff-at-tss-consulting.com
207-925-6014

Login Host: 74.209.46.201
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Fri Aug 21 00:10:04 2009
7, 13 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7L5A2wK014992
7, 13 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 00:10:03 -0500
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240801c6b3dc968790-at-[78.64.120.92]}
7, 13 -- Date: Fri, 21 Aug 2009 00:10:00 -0500
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: jeff-at-tss-consulting.com (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: Research Assistant position
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7L5A2wK014992
==============================End of - Headers==============================




From: Susan.Wert-at-CCHMC.org
Date: Fri, 21 Aug 2009 00:10:38 -0500
Subject: [Microscopy] viaWWW: Durst enlarger available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Susan.Wert-at-CCHMC.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Susan.Wert-at-CCHMC.org
Name: Dr. Susan E. Wert, PhD

Organization: Cincinnati Children's Hospital Medical Center/Research Foundation

Title-Subject: [Filtered] Durst enlarger

Question: We have been forced to shut down our Darkroom. I have a
Durst enlarger complete all lenses that is available to someone that
can use it. Make an offer or contact me for details.





Login Host: 205.142.197.72
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Fri Aug 21 00:10:37 2009
10, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7L5AZnj016718
10, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 00:10:36 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c6b3dcb38e8b-at-[78.64.120.92]}
10, 11 -- Date: Fri, 21 Aug 2009 00:10:34 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: Susan.Wert-at-CCHMC.org (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Durst enlarger available
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Fri, 21 Aug 2009 10:58:45 -0500
Subject: [Microscopy] Carbon error in EDS data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What is the condition of your standards and sample? Were they both carbon coated and coated for the same time? I have seen variation in the intensity of the other elements due to the thickness of the carbon coating. That included increasing the intensities if the C layer is thinner than normal.

The carbon layer resides on the surface, so it probably shouldn't be handled by the matrix correction as if it were distributed uniformly. I normally ignore it. It was probably ignored for the standards.

You haven't said anything about the standards and the software used for matrix correction. That could make a big difference.

I'd be happy to continue the discussion off-line.

Warren

-----Original Message-----
X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
Sent: Thursday, August 20, 2009 4:35 PM
To: wesaia-at-iastate.edu

List,

I have gathered some weight percent compositions of mineral grains in thin sections using EDS and now see error in calculating oxides which I think is due to the inclusion of C in the data set. The C (epoxy mounting material?) should not be part of the mineral composition although I do see a small peak giving me around 10 wt%. If I remove the carbon and redistribute that to the other elements in the composition and convert to oxides my totals are coming out high in the 110 to 117 percent range for elements present.

Element
Wt%
At%
CK
09.26
16.16
OK
34.96
45.79
MgK
09.54
08.22
AlK
02.30
01.78
SiK
25.74
19.21
CaK
13.52
07.07
FeK
04.70
01.76
Matrix
Correction
ZAF


Any thoughts?

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu


==============================Original Headers==============================
14, 36 -- From wesaia-at-iastate.edu Fri Aug 21 10:58:45 2009
14, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
14, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LFwjc0021679
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 10:58:45 -0500
14, 36 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
14, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n7LFwiBM025531
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 10:58:44 -0500
14, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
14, 36 -- id 4ddf_81238826_8e6b_11de_9f95_00137253420a;
14, 36 -- Fri, 21 Aug 2009 10:58:43 -0500
14, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
14, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n7LFwhoX013638
14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 10:58:44 -0500
14, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 36 -- Fri, 21 Aug 2009 10:58:44 -0500
14, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 36 -- Content-class: urn:content-classes:message
14, 36 -- MIME-Version: 1.0
14, 36 -- Content-Type: text/plain;
14, 36 -- charset="iso-8859-1"
14, 36 -- Subject: RE: [Microscopy] Carbon error in EDS data
14, 36 -- Date: Fri, 21 Aug 2009 11:01:18 -0500
14, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270414E812-at-maire.eng.iastate.edu}
14, 36 -- In-Reply-To: {200908202134.n7KLYt7w012683-at-ns.microscopy.com}
14, 36 -- X-MS-Has-Attach:
14, 36 -- X-MS-TNEF-Correlator:
14, 36 -- Thread-Topic: [Microscopy] Carbon error in EDS data
14, 36 -- Thread-Index: Acoh5iIUUC/SDre7RFCwK03BLOmQFQAj9hOg
14, 36 -- References: {200908202134.n7KLYt7w012683-at-ns.microscopy.com}
14, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
14, 36 -- To: {Microscopy-at-microscopy.com}
14, 36 -- X-OriginalArrivalTime: 21 Aug 2009 15:58:44.0206 (UTC) FILETIME=[429680E0:01CA2278]
14, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.8.21.154822
14, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_2000_2999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0'
14, 36 -- Content-Transfer-Encoding: 8bit
14, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7LFwjc0021679
==============================End of - Headers==============================




From: jbricker-at-emqso.com
Date: Fri, 21 Aug 2009 15:46:59 -0500
Subject: [Microscopy] viaWWW: Old JEOL TEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jbricker-at-emqso.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jbricker-at-emqso.com
Name:

Title-Subject: [Filtered] Old JEOL TEM's

Question: Dear readers,

A prior posting from someone hoping to find a new home for their old
JEOL TEM got me thinking...

I'm looking for JEOL TEM's in order to help keep other JEOL TEM's
running. If anyone else happens to have one lying around I would love
to hear from you off-line.

Thank you.
Joe

Login Host: 173.26.214.103
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Aug 21 15:46:59 2009
8, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LKkvwp013214
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 15:46:59 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240806c6b4b7a9a2f8-at-[78.64.120.92]}
8, 11 -- Date: Fri, 21 Aug 2009 15:46:56 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: jbricker-at-emqso.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Old JEOL TEM's
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: halina-at-ucsd.edu
Date: Fri, 21 Aug 2009 15:47:04 -0500
Subject: [Microscopy] viaWWW: Adding digital camera to TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both halina-at-ucsd.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: halina-at-ucsd.edu
Name: Halina Witkiewicz

Organization: PRISM

Title-Subject: [Filtered] Adding digital camera to TEM

Question: Does anybody have an experience with adding a digital
camera to a TEM? My impression from watching this list is that it may
not be an easy task because older microscopes have been offered for
free "to good homes". Am I right?
Thank you for sharing your thoughts if you would, please.

Halina Witkiewicz, Ph.D.
Senior Research Scientist
PRISM
Proteogenomics Research Institute for Systems Medicine
11107 Roselle St.
San Diego, CA 92121

Login Host: 72.34.67.78
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Aug 21 15:47:04 2009
7, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LKl1Gc013240
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 15:47:03 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240807c6b4b7ddaf20-at-[78.64.120.92]}
7, 11 -- Date: Fri, 21 Aug 2009 15:46:59 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: halina-at-ucsd.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Adding digital camera to TEM
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 21 Aug 2009 17:14:10 -0500
Subject: [Microscopy] Re: viaWWW: Adding digital camera to TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Halina,

It should be possible to add a digital camera to most older TEMs.
Normally, you would remove the film camera and install the digital
camera system in its place. Or, with TEMs that have ports above the
viewing chamber (for accommodating a 35 mm film camera) you could
install a slide-in digital camera system at that location.

Digital cameras for TEMs vary in price from $25K up to $150K (or
more). You may be able to buy a used one, even. If you do the
installation yourself, as we did recently, you can get a reasonable
system for $25-30K. It definitely won't be a fancy system, but it will
produce publication quality images.

I believe most people give away older TEMs since parts are no longer
available, they cannot afford to keep them running, or they no longer
use TEM enough to justify keeping one online. Realistically, the
digital camera (even the cheapest one) is worth more than many older
TEMs (even if the TEM is still operational).

John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901


-------------------------

On Fri, Aug 21, 2009 at 3:48 PM, {halina-at-ucsd.edu} wrote:
}
} Email: halina-at-ucsd.edu
} Name: Halina Witkiewicz
}
Question: Does anybody have an experience with adding a digital
} camera to a TEM? My impression from watching this list is that it may
} not be an easy task because older microscopes have been offered for
} free "to good homes". Am I right?
} Thank you for sharing your thoughts if you would, please.
}
} Halina Witkiewicz, Ph.D.
} Senior Research Scientist
} PRISM
} Proteogenomics Research Institute for Systems Medicine
} 11107 Roselle St.
} San Diego, CA 92121

--

==============================Original Headers==============================
9, 18 -- From bozzola-at-siu.edu Fri Aug 21 17:14:09 2009
9, 18 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.149])
9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LME9Tv013539
9, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 17:14:09 -0500
9, 18 -- Received: by qw-out-1920.google.com with SMTP id 5so677174qwf.54
9, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 15:14:09 -0700 (PDT)
9, 18 -- MIME-Version: 1.0
9, 18 -- Received: by 10.224.44.157 with SMTP id a29mr1262298qaf.1.1250892848952; Fri,
9, 18 -- 21 Aug 2009 15:14:08 -0700 (PDT)
9, 18 -- In-Reply-To: {200908212048.n7LKm5Bf015826-at-ns.microscopy.com}
9, 18 -- References: {200908212048.n7LKm5Bf015826-at-ns.microscopy.com}
9, 18 -- Date: Fri, 21 Aug 2009 17:14:08 -0500
9, 18 -- Message-ID: {ebc2299e0908211514w23afcda0u2566922b7f31368e-at-mail.gmail.com}
9, 18 -- Subject: Re: [Microscopy] viaWWW: Adding digital camera to TEM
9, 18 -- From: John Bozzola {bozzola-at-siu.edu}
9, 18 -- To: halina-at-ucsd.edu, MSAListserver {Microscopy-at-microscopy.com}
9, 18 -- Content-Type: text/plain; charset=UTF-8
9, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: axelsson-at-acc.umu.se
Date: Fri, 21 Aug 2009 17:43:26 -0500
Subject: [Microscopy] Re: viaWWW: Old JEOL TEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

I reply on list as there might be more people interested in parts.

I have one complete system that I haven't been using for a couple of
years and a lot of spare parts from two other systems (one 100CX and one
100).
I saved as much as possible from the two dismantled TEM:s to keep my
remaining system running or to sell or trade with people needing spare
parts.

If you or anyone else is looking for anything specific part, let me know.

Regards, Göran Axelsson
Umeå, Sweden

jbricker-at-emqso.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both jbricker-at-emqso.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: jbricker-at-emqso.com
} Name:
}
} Title-Subject: [Filtered] Old JEOL TEM's
}
} Question: Dear readers,
}
} A prior posting from someone hoping to find a new home for their old
} JEOL TEM got me thinking...
}
} I'm looking for JEOL TEM's in order to help keep other JEOL TEM's
} running. If anyone else happens to have one lying around I would love
} to hear from you off-line.
}
} Thank you.
} Joe
}
}


==============================Original Headers==============================
7, 24 -- From axelsson-at-acc.umu.se Fri Aug 21 17:43:26 2009
7, 24 -- Received: from mail.acc.umu.se (mail.acc.umu.se [130.239.18.156])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LMhPi5029055
7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 17:43:26 -0500
7, 24 -- Received: from localhost (localhost [127.0.0.1])
7, 24 -- by amavisd-new (Postfix) with ESMTP id 7716D8F1;
7, 24 -- Sat, 22 Aug 2009 00:43:25 +0200 (MEST)
7, 24 -- X-Virus-Scanned: amavisd-new at acc.umu.se
7, 24 -- Received: from [192.168.1.4] (1-1-10-48a.um.um.bostream.se [82.182.239.55])
7, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
7, 24 -- (No client certificate requested)
7, 24 -- by mail.acc.umu.se (Postfix) with ESMTPS id 344748EE;
7, 24 -- Sat, 22 Aug 2009 00:43:13 +0200 (MEST)
7, 24 -- Message-ID: {4A8F2301.4080009-at-acc.umu.se}
7, 24 -- Date: Sat, 22 Aug 2009 00:43:13 +0200
7, 24 -- From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se}
7, 24 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318)
7, 24 -- MIME-Version: 1.0
7, 24 -- To: jbricker-at-emqso.com, microscopy-at-microscopy.com
7, 24 -- Subject: Re: [Microscopy] viaWWW: Old JEOL TEM's
7, 24 -- References: {200908212047.n7LKl9d9013342-at-ns.microscopy.com}
7, 24 -- In-Reply-To: {200908212047.n7LKl9d9013342-at-ns.microscopy.com}
7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 24 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Fri, 21 Aug 2009 17:47:54 -0500
Subject: [Microscopy] Re: viaWWW: Adding digital camera to TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Last year I made a rig to hold a "35mm" standard digital camera with
macro lens precisely aligned and angled to image the fluorescent screen
on my TEM. The $700 camera produces 11 megapixel images. The results
were slightly distorted since the screen is in the 45 degree viewing
position but otherwise no one could distinguish the resulting image from
one taken with standard film and scanned at 1200dpi. Not all TEMs are
equally adapatable but it is possible to get good results for about $1000!

Larry

halina-at-ucsd.edu wrote:
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both halina-at-ucsd.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: halina-at-ucsd.edu
} Name: Halina Witkiewicz
}
} Organization: PRISM
}
} Title-Subject: [Filtered] Adding digital camera to TEM
}
} Question: Does anybody have an experience with adding a digital
} camera to a TEM? My impression from watching this list is that it may
} not be an easy task because older microscopes have been offered for
} free "to good homes". Am I right?
} Thank you for sharing your thoughts if you would, please.
}
} Halina Witkiewicz, Ph.D.
} Senior Research Scientist
} PRISM
} Proteogenomics Research Institute for Systems Medicine
} 11107 Roselle St.
} San Diego, CA 92121
}
} Login Host: 72.34.67.78
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Fri Aug 21 15:47:04 2009
} 7, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LKl1Gc013240
} 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 15:47:03 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240807c6b4b7ddaf20-at-[78.64.120.92]}
} 7, 11 -- Date: Fri, 21 Aug 2009 15:46:59 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: halina-at-ucsd.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Adding digital camera to TEM
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
} .
}

--
Larry Ackerman, Specialist
UCSF, Dept. of Anatomy, Rm S1347
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400

==============================Original Headers==============================
6, 33 -- From Larry.Ackerman-at-ucsf.edu Fri Aug 21 17:47:54 2009
6, 33 -- Received: from mail.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LMlrNT007039
6, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 17:47:54 -0500
6, 33 -- X-ASG-Debug-ID: 1250894872-1c3f01610000-1DjkGe
6, 33 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
6, 33 -- Received: from EXHT02.net.ucsf.edu (localhost [127.0.0.1])
6, 33 -- by mail.ucsf.edu (Spam & Virus Firewall) with ESMTP
6, 33 -- id 27B18D99F38; Fri, 21 Aug 2009 15:47:52 -0700 (PDT)
6, 33 -- Received: from EXHT02.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by mail.ucsf.edu with ESMTP id EgqUjqVRh9fgDf2a; Fri, 21 Aug 2009 15:47:52 -0700 (PDT)
6, 33 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
6, 33 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
6, 33 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
6, 33 -- Received: from Ralston-Lab-Larry-Ackerman.local (128.218.123.88) by
6, 33 -- EXHT02.net.ucsf.edu (64.54.247.219) with Microsoft SMTP Server id 8.1.393.1;
6, 33 -- Fri, 21 Aug 2009 15:47:52 -0700
6, 33 -- Message-ID: {4A8F2400.1060409-at-ucsf.edu}
6, 33 -- Date: Fri, 21 Aug 2009 15:47:28 -0700
6, 33 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
6, 33 -- Reply-To: larry.ackerman-at-ucsf.edu
6, 33 -- Organization: UCSF, NeuroAnatomy
6, 33 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707)
6, 33 -- MIME-Version: 1.0
6, 33 -- To: "halina-at-ucsd.edu" {halina-at-ucsd.edu} , {Microscopy-at-microscopy.com}
6, 33 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW: Adding digital camera to TEM
6, 33 -- Subject: Re: [Microscopy] viaWWW: Adding digital camera to TEM
6, 33 -- References: {200908212055.n7LKtTrL000317-at-ns.microscopy.com}
6, 33 -- In-Reply-To: {200908212055.n7LKtTrL000317-at-ns.microscopy.com}
6, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
6, 33 -- Content-Transfer-Encoding: 7bit
6, 33 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
6, 33 -- X-Barracuda-Start-Time: 1250894873
6, 33 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Fri, 21 Aug 2009 18:43:04 -0500
Subject: [Microscopy] street camera TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was wondering if anyone had done what Larry Ackerman describes. I haven't
gotten around to making the rigid frame, but got good pictures just by
holding the camera against the glass. The distortion Larry mentions can be
easily corrected in Photoshop by multiplying the distorted dimension of the
photo by the cosine of the angle of your screen, for example .707 if your
screen is a5 45 degrees. You have to uncheck the "constrain dimensions" box
in Photoshop Image -- Image Size.

Ralph Common


==============================Original Headers==============================
3, 31 -- From rcommon-at-msu.edu Fri Aug 21 18:43:04 2009
3, 31 -- Received: from sys07.mail.msu.edu (sys07.mail.msu.edu [35.9.75.107])
3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LNh4YL026671
3, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 18:43:04 -0500
3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=msu.edu;
3, 31 -- s=mail; h=From:To:Subject:Date:Message-ID:MIME-Version:
3, 31 -- Content-Type:Content-Transfer-Encoding; bh=VfuPcIfXY2DaBpfusj23J
3, 31 -- t5fJqp6EVugM7uuCoJZ9B4=; b=La8tLy2cjJ9Xm9Mk0Bnh8ZJT/9EXb+sTRzrCu
3, 31 -- t4xPGhn7+gC1vBNGwL0GbOdgbq1ixSTShDmfVBNGoWu0flBIoGZkSpQU1QVM3Iac
3, 31 -- srY3Te8EfBPb1PXE7MVqkuJ3TYryxNJ/MqLBWPc4xeAccKAorzkFoXvvmWFK7ySO
3, 31 -- UZ1+0E=
3, 31 -- Received: from [35.9.122.125] (helo=emlab)
3, 31 -- by sys07.mail.msu.edu with esmtpsa (Exim 4.69 #1)
3, 31 -- (TLSv1:RC4-MD5:128)
3, 31 -- id 1Medl9-0007g8-MF
3, 31 -- for Microscopy-at-microscopy.com; Fri, 21 Aug 2009 19:43:03 -0400
3, 31 -- From: "Ralph Common" {rcommon-at-msu.edu}
3, 31 -- To: {Microscopy-at-microscopy.com}
3, 31 -- Subject: street camera TEM
3, 31 -- Date: Fri, 21 Aug 2009 19:41:55 -0400
3, 31 -- Message-ID: {00bb01ca22b8$f845a0c0$7d7a0923-at-msu.edu}
3, 31 -- MIME-Version: 1.0
3, 31 -- Content-Type: text/plain;
3, 31 -- charset="iso-8859-1"
3, 31 -- Content-Transfer-Encoding: 7bit
3, 31 -- X-Priority: 3 (Normal)
3, 31 -- X-MSMail-Priority: Normal
3, 31 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
3, 31 -- Importance: Normal
3, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1983
3, 31 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 21 Aug 2009 19:28:44 -0500
Subject: [Microscopy] Re: street camera TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm sure purists would give many reasons why this is not a good
approach (low resolution of viewing screen, dirt on screen, etc.). But
one cannot argue with the price of this system. Furthermore, it may be
perfectly adequate for capturing study images, and even (gasp, gasp)
publishable ones. Obviously, markings on the screen (cross hairs,
bulls eyes, etc.) would nix some uses.

Go for it! After all, if it doesn't work out, you still have a digital
camera for lab use.


--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts (IMAGE)
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901

--------------------------------------------

On Fri, Aug 21, 2009 at 6:43 PM, {rcommon-at-msu.edu} wrote:

} I was wondering if anyone had done what Larry Ackerman describes.  I haven't
} gotten around to making the rigid frame, but got good pictures just by
} holding the camera against the glass.  The distortion Larry mentions can be
} easily corrected in Photoshop by multiplying the distorted dimension of the
} photo by the cosine of the angle of your screen, for example .707 if your
} screen is a5 45 degrees.  You have to uncheck the "constrain dimensions" box
} in Photoshop Image -- Image Size.
}
} Ralph Common


==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Fri Aug 21 19:28:43 2009
8, 19 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.148])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7M0ShCt009951
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 19:28:43 -0500
8, 19 -- Received: by qw-out-1920.google.com with SMTP id 5so718718qwf.54
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 17:28:43 -0700 (PDT)
8, 19 -- MIME-Version: 1.0
8, 19 -- Received: by 10.224.29.203 with SMTP id r11mr1293111qac.110.1250900923357;
8, 19 -- Fri, 21 Aug 2009 17:28:43 -0700 (PDT)
8, 19 -- In-Reply-To: {200908212343.n7LNhsFt027974-at-ns.microscopy.com}
8, 19 -- References: {200908212343.n7LNhsFt027974-at-ns.microscopy.com}
8, 19 -- Date: Fri, 21 Aug 2009 19:28:43 -0500
8, 19 -- Message-ID: {ebc2299e0908211728k4433528bm1443f9b3ec997647-at-mail.gmail.com}
8, 19 -- Subject: Re: [Microscopy] street camera TEM
8, 19 -- From: John Bozzola {bozzola-at-siu.edu}
8, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
8, 19 -- Content-Type: text/plain; charset=UTF-8
8, 19 -- Content-Transfer-Encoding: 8bit
8, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7M0ShCt009951
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Sat, 22 Aug 2009 04:30:50 -0500
Subject: [Microscopy] Re: viaWWW: Adding digital camera to TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Adding a newer digital camera to the wide angle side port of an older
TEM (which one in specific?) is not too complicated. I did this various
times on Zeiss EM10s and on Philips / FEI EM 400/420.
There are interface boards available to get an automatic reading of high
voltage and magnification values to simplify working with cameras. These
boards work with GATAN, Olympus-SoftImaging and any other camera
software which is able to handle a few bytes being transfered via a
RS232 port...
These interface boards are available from me, I also do complete
installations of camera, interfaces and software.
When I can be of further help, ask me offline.

Best regards,
Stefan


--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------



halina-at-ucsd.edu schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both halina-at-ucsd.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: halina-at-ucsd.edu
} Name: Halina Witkiewicz
}
} Organization: PRISM
}
} Title-Subject: [Filtered] Adding digital camera to TEM
}
} Question: Does anybody have an experience with adding a digital
} camera to a TEM? My impression from watching this list is that it may
} not be an easy task because older microscopes have been offered for
} free "to good homes". Am I right?
} Thank you for sharing your thoughts if you would, please.
}
} Halina Witkiewicz, Ph.D.
} Senior Research Scientist
} PRISM
} Proteogenomics Research Institute for Systems Medicine
} 11107 Roselle St.
} San Diego, CA 92121
}
} Login Host: 72.34.67.78
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Fri Aug 21 15:47:04 2009
} 7, 11 -- Received: from [78.64.120.92] (msdvpn072.msd.anl.gov [130.202.238.72])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7LKl1Gc013240
} 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2009 15:47:03 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240807c6b4b7ddaf20-at-[78.64.120.92]}
} 7, 11 -- Date: Fri, 21 Aug 2009 15:46:59 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: halina-at-ucsd.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Adding digital camera to TEM
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
9, 22 -- From stefan.diller-at-t-online.de Sat Aug 22 04:30:49 2009
9, 22 -- Received: from mailout03.t-online.de (mailout03.t-online.de [194.25.134.81])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7M9Unvs020621
9, 22 -- for {microscopy-at-microscopy.com} ; Sat, 22 Aug 2009 04:30:49 -0500
9, 22 -- Received: from fwd00.aul.t-online.de
9, 22 -- by mailout03.t-online.de with smtp
9, 22 -- id 1Memvw-0005yV-01; Sat, 22 Aug 2009 11:30:48 +0200
9, 22 -- Received: from [192.168.2.101] (bRB-qeZSrhpq10GeleChtl2DocqZ42hOMAQNWfXQnGj6q8co-TsdKSTwW4HICJBgSn-at-[93.222.96.39]) by fwd00.aul.t-online.de
9, 22 -- with esmtp id 1Memvp-0DwooS0; Sat, 22 Aug 2009 11:30:41 +0200
9, 22 -- Message-ID: {4A8FBAC1.5060403-at-t-online.de}
9, 22 -- Date: Sat, 22 Aug 2009 11:30:41 +0200
9, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
9, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
9, 22 -- MIME-Version: 1.0
9, 22 -- To: microscopy-at-microscopy.com
9, 22 -- Subject: Re: [Microscopy] viaWWW: Adding digital camera to TEM
9, 22 -- References: {200908212053.n7LKrH3Z026903-at-ns.microscopy.com}
9, 22 -- In-Reply-To: {200908212053.n7LKrH3Z026903-at-ns.microscopy.com}
9, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-ID: bRB-qeZSrhpq10GeleChtl2DocqZ42hOMAQNWfXQnGj6q8co-TsdKSTwW4HICJBgSn
9, 22 -- X-TOI-MSGID: 0b970d6e-26b5-45b8-ad21-5b9b815755fd
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Mon, 24 Aug 2009 08:04:12 -0500
Subject: [Microscopy] Re: viaWWW: Adding digital camera to TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Halina-
We just added a digital camera to our (very old) JEM 100 CX-II. The
only hang-up was that the manufacturer didn't have a flange fitting
for our column when we ordered the camera, since they hadn't
anticipated putting a camera on such an old 'scope. The sales rep &
I found 3 other labs with the same model EM that were interested, so
the company made the fitting. It took a while for me, since I was
their first, but now they have the spex & any subsequent orders
should go quickly. Now if I can just find the time to learn how to
use the darned software!
Lee



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
7, 34 -- From lcgould-at-med.cornell.edu Mon Aug 24 08:04:11 2009
7, 34 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7OD4Axm000510
7, 34 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2009 08:04:11 -0500
7, 34 -- MIME-version: 1.0
7, 34 -- Content-transfer-encoding: 7BIT
7, 34 -- Content-type: text/plain; charset=us-ascii; format=flowed
7, 34 -- Received: from mpx5.med.cornell.edu ([140.251.11.120])
7, 34 -- by mail-gw2.med.cornell.edu
7, 34 -- (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 14 2008; 32bit))
7, 34 -- with ESMTP id {0KOV00HAUTMVKD10-at-mail-gw2.med.cornell.edu} for
7, 34 -- microscopy-at-microscopy.com; Mon, 24 Aug 2009 09:04:07 -0400 (EDT)
7, 34 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
7, 34 -- by mpx5.med.cornell.edu
7, 34 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
7, 34 -- with ESMTPA id {0KOV0068PTMT6U60-at-mpx5.med.cornell.edu} ; Mon,
7, 34 -- 24 Aug 2009 09:04:07 -0400 (EDT)
7, 34 -- X-PMX-Version: 5.5.7.378829, Antispam-Engine: 2.7.2.376379,
7, 34 -- Antispam-Data: 2009.8.24.125117
7, 34 -- X-Perlmx-Spam: Gauge=X, , Probability=10%, Report=' TO_IN_SUBJECT 0.5,
7, 34 -- BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
7, 34 -- TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0,
7, 34 -- __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0,
7, 34 -- __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
7, 34 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0,
7, 34 -- __URI_NS '
7, 34 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
7, 34 -- Message-id: {p0623095bc6b83f6a8046-at-[140.251.48.23]}
7, 34 -- In-reply-to: {200908212058.n7LKwHPw009227-at-ns.microscopy.com}
7, 34 -- References: {200908212058.n7LKwHPw009227-at-ns.microscopy.com}
7, 34 -- Date: Mon, 24 Aug 2009 09:03:58 -0400
7, 34 -- To: halina-at-ucsd.edu, Microscopy Listserver {microscopy-at-microscopy.com}
7, 34 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
7, 34 -- Subject: Re: [Microscopy] viaWWW: Adding digital camera to TEM
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Mon, 24 Aug 2009 10:09:19 -0500
Subject: [Microscopy] Sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

I am impressed (and grateful!) with responses on my previous question,
so I am ready to ask a new one.

We are ready to replace our old sputter coater with rotary pump. We are
coating our specimens with Au/Pd alloy, which is fine up to
magnifications 50,000-100,000, and these mags are about the maximum our
field emission XL30 can reach. Additionally, we use "etch" mode to make
coated TEM grids hydrophilic.

Some of our specimens consist of fine "network" of thin fibers (collagen
in etched bone and teeth, bone scaffolds) or of piles of spherical micro
or nano particles. To decrease charging, we coat specimens like these
2-3 times, manually changing their position in between runs, so that
they would be coated under different angles.

Additionally, our carbon coater is aging, and for our new sputter coater
I'd like to have a possibility of switching to carbon coating when
necessary.

So, my "ideal" new coater should have simple pumping system (to keep
price down), tilting/rotating stage (for tough to coat specimens),
carbon coating attachment and "etch" mode. Unfortunately, I have found
out there are no such coater on a market. The main problem is that
"etch" mode is incompatible with tilting/rotating stage.

So, my main two questions are:
1. Is tilting/rotating stage really helpful in coating tough specimens?
2. To keep tilting/rotating stage in a coater, should I by separate glow
discharge unit for TEM grids, which costs almost as much as sputter
coater itself, or should I switch to a cheaper but new for me method of
making TEM grids hydrophilic (which one)?

Additionally, I would like to learn opinions on different brands of
sputter coaters (off-list), especially on their appetite for targets.

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
14, 23 -- From DusevichV-at-umkc.edu Mon Aug 24 10:09:19 2009
14, 23 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155])
14, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7OF9HnA019776
14, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Aug 2009 10:09:18 -0500
14, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
14, 23 -- Mon, 24 Aug 2009 10:09:16 -0500
14, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
14, 23 -- Content-class: urn:content-classes:message
14, 23 -- MIME-Version: 1.0
14, 23 -- Content-Type: text/plain;
14, 23 -- charset="us-ascii"
14, 23 -- Subject: Sputter coater
14, 23 -- Date: Mon, 24 Aug 2009 10:09:15 -0500
14, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB8B6-at-KC-MSX1.kc.umkc.edu}
14, 23 -- X-MS-Has-Attach:
14, 23 -- X-MS-TNEF-Correlator:
14, 23 -- Thread-Topic: Sputter coater
14, 23 -- Thread-Index: AcokzNiXpKY13OKtTGqAvazGZljSQg==
14, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
14, 23 -- To: {Microscopy-at-microscopy.com}
14, 23 -- X-OriginalArrivalTime: 24 Aug 2009 15:09:16.0219 (UTC) FILETIME=[D8C4E0B0:01CA24CC]
14, 23 -- Content-Transfer-Encoding: 8bit
14, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7OF9HnA019776
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 24 Aug 2009 14:23:33 -0500
Subject: [Microscopy] Prices on Carbon film Extraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone,
I'm trying to get a cost estimate of what an outside lab would charge for a
carbon film extraction on metal.

For those interested, you polish a metal sample to 1um, lightly etch and
carbon coat. The coating is scored into little squares and the sample is
place in a electrolytic etch. The metal is etched away and the carbon film
containing the inclusions floats free. You them spend a little time
chasing the film with a TEM grid. Hopefully the features of interest are
suspended over a opening in the grid and the SEM can get a nice clean
spectra and reasonable images.

I have the SEM, but the carbon film and etch need some outside support, I'm
working up a budget. My internal customer would like at least a sample a
week for a year.

Any thoughts would be welcome..............

Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
8, 21 -- From frank_karl-at-lincolnelectric.com Mon Aug 24 14:23:32 2009
8, 21 -- Received: from lincolnelectric.com ([64.109.211.114])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7OJNVn6010731
8, 21 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2009 14:23:31 -0500
8, 21 -- Subject: Prices on Carbon film Extraction
8, 21 -- To: Microscopy-at-microscopy.com
8, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
8, 21 -- Message-ID: {OF52AC837B.4314A70E-ON8525761C.00691B5D-8525761C.006A80B6-at-lincolnelectric.com}
8, 21 -- Date: Mon, 24 Aug 2009 15:23:39 -0400
8, 21 -- From: Frank_Karl-at-lincolnelectric.com
8, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 21 -- 07, 2008) at 08/24/2009 03:23:18 PM,
8, 21 -- CD-MIME complete at 08/24/2009 03:23:18 PM,
8, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 21 -- 07, 2008) at 08/24/2009 03:23:18 PM,
8, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
8, 21 -- 07, 2008) at 08/24/2009 03:23:18 PM,
8, 21 -- Serialize complete at 08/24/2009 03:23:18 PM
8, 21 -- MIME-Version: 1.0
8, 21 -- Content-Type: text/plain;
8, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: 08/20/2009 11:53AM
Subject: [Microscopy] LM user needs help for Polaroid DMC-1e software issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Assuming the old PC is genuinely beyond repair [Motherboard shot] and
doesn't just need say a new PSU or hard drive, if the camera is meeting your
users needs rather than junk a decent [for its day] working 1MP colour
camera, you could alternatively just buy a 2nd hand [probably 1GHz Intel
PIII or 1.2GHz AMD Athlon] PC from eBay and run Windows 98SE on that after a
clean install. I'd avoid dual booting with XP though as Windows 98 often
seemed to always screw up after a few months. Pick any 6-8 year old computer
and Win 98 drivers will be available for most PC hardware [check out the
motherboard at the manufacturer's website for downloadable drivers, and what
PCI/ISA/AGP Graphics slots is has]. Plus you can get a top of the range PC
from the time for peanuts. Cost, about £60 in the UK, if you buy local and
collect. Use the old monitor and keyboard/mouse [or go wild and upgrade the
VGA monitor to a 19" LCD [with VGA in] and get an optical mouse as well].

Be discerning though and I'd pick a home PC that’s been replaced with a
modern PC [far less wear and tear]. Also check that eBay feedback. I tend to
upgrade the AGP 2-4x graphics card to the best 2002 could offer as well for
another £20 [and a similar top grade £10 S/H Creative sound card if for our
pre-school bouncing beavers software use]. I also try and avoid Dell and
pick a PC with a commercial motherboard that's better supported for drivers
on-line. Ideally stuff in plenty of matched second hand memory [up to
typically 512Mb, perhaps use 2nd hand Crucial where you can check
compatibility/max memory more easily] and perhaps a new PATA hard drive
[that will soup things up - but beware of any motherboard BIOS limitations
on capacity, i.e 8.4Gb or much more likely 120Gb - again check on-line, and
there are software hard drive 'fixes' from some HD manufacturers, but I'd
stick to a new 120Gb, they are still about]. You might be banned from the
works intranet though and have to run standalone [but at least Windows 98
supports USB2 unlike NT - so also fit a Belkin USB2 PCI card asap]. Shame
that Windows 98SE is a fairly naff unstable OS compared to XP [caused most
likely by Microsoft over-burdening it with security patches, although I'm
sure that's not to deliberately tempt you to upgrade to XP]. Oh and Hoover
out the PC dust bunnies [avoid brushing the electronic circuits, static and
all that, so use a dust blower and the hovering Hoover on those]. You might
need an identical S/H SCSI-2 card if the old one's shot, but like sound
cards and graphics card they generally run forever in PC terms. Win98 SCSI
drivers will not run under XP and manufacturers rarely if ever bother to
update the drivers for old SCSI cards for a new OS.

Otherwise spend presumably a lot more than $100 and buy that new camera
[with more than a 2MP resolution].

Keith


---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/cytogenetics/

-----Original Message-----
X-from: gas19-at-chrysler.com [mailto:gas19-at-chrysler.com]
Sent: 20 August 2009 17:41
To: kjmorris-at-well.ox.ac.uk


We had the same issue a few years ago. We had all of our cameras on
Windows 2000 computers. One of the computers died, so we tried it on a
Windows XP computer, and could not get it to work. Luckily we had a slow
transition from Windows 2000 to Windows XP, and were able to upgrade all of
our cameras without loosing function for too long.

I think you need to buy a new camera.

Gerald Shulke
Materials Engineering Specialist
Materials Characterization Labs
Chrysler Group LLC



-----William.H.Roberts-at-USA.dupont.com wrote: -----


To: gas19-at-chrysler.com
X-from: William.H.Roberts-at-USA.dupont.com




----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear Listers,

Does anyone know if updated software is available to run the Polaroid
DMC-1e camera using a computer with Windows XP operating system? The
original computer was a Win98 version, but it died. Polaroid's customer
support telephone message says that they no longer support the system. Do

I need a new camera (I know that I do, just need confirmation.)?

TIA Bill Roberts

This communication is for use by the intended recipient and contains
information that may be Privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender by
return e-mail and delete this e-mail from your system. Unless explicitly
and conspicuously designated as "E-Contract Intended", this e-mail does
not constitute a contract offer, a contract amendment, or an acceptance
of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html


==============================Original
Headers==============================
8, 28 -- From William.H.Roberts-at-USA.dupont.com Thu Aug 20 10:47:37 2009
8, 28 -- Received: from mail203.messagelabs.com (mail203.messagelabs.com
[216.82.254.243])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n7KFlakm018375
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 10:47:37
-0500
8, 28 -- X-VirusChecked: Checked
8, 28 -- X-Env-Sender: William.H.Roberts-at-USA.dupont.com
8, 28 -- X-Msg-Ref:
server-12.tower-203.messagelabs.com!1250783255!47774296!1
8, 28 -- X-StarScan-Version: 6.1.3; banners=-,-,-
8, 28 -- X-Originating-IP: [52.129.16.69]
8, 28 -- Received: (qmail 16278 invoked from network); 20 Aug 2009 15:47:35
-0000
8, 28 -- Received: from unknown (HELO demhub21.lvs.dupont.com)
(52.129.16.69)
8, 28 -- by server-12.tower-203.messagelabs.com with SMTP; 20 Aug 2009
15:47:35 -0000
8, 28 -- Received: from unknown (HELO demhub1.lvs.dupont.com)
([52.99.10.45])
8, 28 -- by demhub21a.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:35
-0400
8, 28 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com}
8, 28 -- Received: from cdcln05.lvs.dupont.com ([52.99.26.10])
8, 28 -- by demhub1.lvs.dupont.com with ESMTP; 20 Aug 2009 11:47:34 -0400
8, 28 -- To: Microscopy-at-microscopy.com
8, 28 -- Subject: LM user needs help for Polaroid DMC-1e software issue
8, 28 -- MIME-Version: 1.0
8, 28 -- X-Mailer: Lotus Notes Release 7.0.1 CCH2 May 01, 2006
8, 28 -- Message-ID: {OF77C1DB99.81A8CB13-ON85257618.00560DA5-85257618.0056
BF74-at-CDCLN05.LVS.DUPONT.COM}
8, 28 -- Date: Thu, 20 Aug 2009 11:47:31 -0400
8, 28 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release
8.5 HF460|May 15, 2009) at
8, 28 -- 08/20/2009 11:47:34 AM,
8, 28 -- Serialize complete at 08/20/2009 11:47:34 AM
8, 28 -- Content-Transfer-Encoding: 7bit
8, 28 -- Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 28 -- From gas19-at-chrysler.com Thu Aug 20 11:35:40 2009
25, 28 -- Received: from shbmap02.extra.chrysler.com
(shbmap02.out.extra.chrysler.com [129.9.168.37])
25, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n7KGZdMT015875
25, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 11:35:39
-0500
25, 28 -- Received: from shbmap04.shdc.chrysler.com (unknown [53.28.160.92])
25, 28 -- by shbmap02.extra.chrysler.com (Symantec Mail Security) with
ESMTP id 860CC4E4003
25, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2009 12:35:39
-0400 (EDT)
25, 28 -- X-AuditID: 8109a824-a99d6bb00000726d-40-4a8d7b5b9ad1
25, 28 -- Received: from sodddg01.wk.dcx.com
(odmcp057-vip3.oddc.chrysler.com [53.231.74.218])
25, 28 -- by shbmap04.shdc.chrysler.com (Symantec Brightmail Gateway)



From: kjmorris-at-well.ox.ac.uk
Date: 08/20/2009 11:53AM
Subject: [Microscopy] LM user needs help for Polaroid DMC-1e software issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



==============================Original Headers==============================
38, 23 -- From kjmorris-at-well.ox.ac.uk Tue Aug 25 06:30:27 2009
38, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
38, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7PBUP1R009901
38, 23 -- for {microscopy-at-microscopy.com} ; Tue, 25 Aug 2009 06:30:26 -0500
38, 23 -- Received: from dhcp285.well.ox.ac.uk ([129.67.45.128] helo=CytoWhizz)
38, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
38, 23 -- id 1MfuEL-0008Jr-2s
38, 23 -- for microscopy-at-microscopy.com; Tue, 25 Aug 2009 12:30:25 +0100
38, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
38, 23 -- To: {microscopy-at-microscopy.com}
38, 23 -- References: {200908201640.n7KGebCh026358-at-ns.microscopy.com}
38, 23 -- In-Reply-To: {200908201640.n7KGebCh026358-at-ns.microscopy.com}
38, 23 -- Subject: RE: [Microscopy] Re: LM user needs help for Polaroid DMC-1e software issue
38, 23 -- Date: Tue, 25 Aug 2009 12:30:24 +0100
38, 23 -- Message-ID: {001901ca2577$703025e0$509071a0$-at-ox.ac.uk}
38, 23 -- MIME-Version: 1.0
38, 23 -- Content-Type: text/plain;
38, 23 -- charset="iso-8859-1"
38, 23 -- X-Mailer: Microsoft Office Outlook 12.0
38, 23 -- Thread-Index: AcohtPKDC1iuy9nLRG6qztEFFcQZXQAjfKiw
38, 23 -- Content-Language: en-gb
38, 23 -- Content-Transfer-Encoding: 8bit
38, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7PBUP1R009901
==============================End of - Headers==============================




From: kusum45-at-yahoo.com
Date: Tue, 25 Aug 2009 16:40:36 -0500
Subject: [Microscopy] viaWWW: JEOL 100CX TEM/STEM Available - Follow up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7PLeagl024510
for {MicroscopyListserverArchive-at-microscopy.com} ; Tue, 25 Aug 2009 16:40:36 -0500
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n7PLeaLg024508;
Tue, 25 Aug 2009 16:40:36 -0500

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kusum45-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kusum45-at-yahoo.com
Name: Vin Berry

Organization: West Virginia University, Morgantown, WV

Title-Subject: [Filtered] JEOL 100CX TEM/STEM Available - Follow up

Question: We have a JEOL 100CX TEM/STEM available immediately. The
instrument is in excellent running condition. It was in service
contract all its life till Jan.2009. It is designed for EDX though
EDX unit is not presently hooked up. There is a low resolution GATAN
camera attached on the side with a monitor and is in good working
condition The camera was hooked up for image capture but not so now.
The scope has W and LaB6 filament holders. The instrument is
available with all the accessories and spare parts we have including
the chiller. The interested party will be responsible for dismantling
and transportation. Those interested are invited to contact me by
email or telephone, preferably the cell # with the price they are
willing to pay.

Vin Berry, Ph.D.
Dept of Chemical Engineering,
WV University

Home: 304-428-6961
Cell: 304-615-1244


Login Host: 173.81.65.144
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Aug 25 16:40:35 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7PLeXmk024497
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 25 Aug 2009 16:40:35 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240802c6ba0aaa2619-at-[206.69.208.22]}
9, 11 -- Date: Tue, 25 Aug 2009 16:40:32 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: kusum45-at-yahoo.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: JEOL 100CX TEM/STEM Available - Follow up
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tclason-at-uvm.edu
Date: Tue, 25 Aug 2009 16:40:59 -0500
Subject: [Microscopy] viaWWW: Coverslip adhesion for TIRF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tclason-at-uvm.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tclason-at-uvm.edu
Name: Todd Clason

Organization: The University of Vermont

Title-Subject: [Filtered] Coverslip adhesion for TIRF

Question: I have a researcher trying to get small parenchymal cells,
essentially smooth muscle cells, to adhere to a glass coverslip for
TIRF. She's had good luck recording sparklets in TIRF with larger
cells, but these have fewer or no good contacts with the coverslip.
Is polylysine the preferred choice?

Thank you for any ideas!

Login Host: 132.198.70.14
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Aug 25 16:40:59 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7PLewa5024928
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 25 Aug 2009 16:40:59 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240803c6ba0ad730cd-at-[206.69.208.22]}
7, 11 -- Date: Tue, 25 Aug 2009 16:40:57 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: tclason-at-uvm.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Coverslip adhesion for TIRF
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 25 Aug 2009 16:45:52 -0500
Subject: [Microscopy] Cryo equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Being on a shopping spree, I am posting more questions concerning EM
equipment, this time for cryo-preparation. We are going to prepare
specimens by freeze substitution.

We are thinking about buying a High pressure freezer. It comes in two
flavors: with or without rapid transfer system. Price difference is
substantial. So, my question: How essential is rapid transfer system?
What are advantages of having it?

My second question is about freeze substitution system. There is an
option for it: stereo microscope. I just do not understand what I can
see during embedding process. How much am I wrong?

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



==============================Original Headers==============================
10, 23 -- From DusevichV-at-umkc.edu Tue Aug 25 16:45:52 2009
10, 23 -- Received: from kc-msxproto3.kc.umkc.edu (smtp2.exchange.umkc.edu [134.193.44.10])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7PLjp5E004680
10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2009 16:45:52 -0500
10, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 23 -- Tue, 25 Aug 2009 16:45:51 -0500
10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 23 -- Content-class: urn:content-classes:message
10, 23 -- MIME-Version: 1.0
10, 23 -- Content-Type: text/plain;
10, 23 -- charset="us-ascii"
10, 23 -- Subject: Cryo equipment
10, 23 -- Date: Tue, 25 Aug 2009 16:45:51 -0500
10, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB8BE-at-KC-MSX1.kc.umkc.edu}
10, 23 -- X-MS-Has-Attach:
10, 23 -- X-MS-TNEF-Correlator:
10, 23 -- Thread-Topic: Cryo equipment
10, 23 -- thread-index: AcolzWoFffGc9u5yRMWRulkE7jT3dw==
10, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
10, 23 -- To: {Microscopy-at-microscopy.com}
10, 23 -- X-OriginalArrivalTime: 25 Aug 2009 21:45:51.0607 (UTC) FILETIME=[6A56B070:01CA25CD]
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7PLjp5E004680
==============================End of - Headers==============================




From: gary-at-perfendo.com
Date: Tue, 25 Aug 2009 21:07:07 -0500
Subject: [Microscopy] viaWWW: Available Biorad 1000 confocal Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gary-at-perfendo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gary-at-perfendo.com
Name: Gary B. Carr

Organization: PERF

Title-Subject: [Filtered] Available Biorad 1000 confocal Microscope

Question: We have a working Biorad 1000 MRC confocal microscope with
recently rebuilt laser available to a good home. Contact me off-list
for particulars.

Login Host: 74.7.224.162
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Aug 25 21:07:07 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7Q277LA025303
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 25 Aug 2009 21:07:07 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6ba4939cff5-at-[206.69.208.22]}
6, 11 -- Date: Tue, 25 Aug 2009 21:07:06 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: gary-at-perfendo.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Available Biorad 1000 confocal Microscope
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: graham.knott-at-epfl.ch
Date: Wed, 26 Aug 2009 01:48:40 -0500
Subject: [Microscopy] re: Cryo equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Vladimir,

I know this does not directly answer your question, and I certainly
don't want to spoil the shopping spree, but I recently saw a
remarkable piece of work published in the Journal of Microscopy by Jan
Leunissen and Hong Yi (vol 235, issue 1, pg 25). They described a
technique of self-pressurised freezing where capillary tubes
containing different samples are simply plunged into cryogens such as
liquid propane. The images they show are excellent and certainly make
you think if you need to prepare certain types of material by freezing.

Regards,
Graham


--------------------------
Graham Knott PhD
Senior Scientist, Head of Bio EM Facility
Centre of Electron Microscopy, EPFL
Lausanne
Switzerland CH 1015
Tel. +41 21 6931862




On Aug 25, 2009, at 11:53 PM, DusevichV-at-umkc.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Greetings,
}
} Being on a shopping spree, I am posting more questions concerning EM
} equipment, this time for cryo-preparation. We are going to prepare
} specimens by freeze substitution.
}
} We are thinking about buying a High pressure freezer. It comes in two
} flavors: with or without rapid transfer system. Price difference is
} substantial. So, my question: How essential is rapid transfer system?
} What are advantages of having it?
}
} My second question is about freeze substitution system. There is an
} option for it: stereo microscope. I just do not understand what I can
} see during embedding process. How much am I wrong?
}
} Thank you,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy

==============================Original Headers==============================
9, 17 -- From graham.knott-at-epfl.ch Wed Aug 26 01:48:40 2009
9, 17 -- Received: from smtp3.epfl.ch (smtp3.epfl.ch [128.178.224.226])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7Q6mdQ8015300
9, 17 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 01:48:40 -0500
9, 17 -- Received: (qmail 32077 invoked by uid 107); 26 Aug 2009 06:48:38 -0000
9, 17 -- X-Virus-Scanned: ClamAV
9, 17 -- Received: from sv4-77.epfl.ch (128.178.193.77) (authenticated)
9, 17 -- by smtp3.epfl.ch (AngelmatoPhylax SMTP proxy); Wed, 26 Aug 2009 08:48:38 +0200
9, 17 -- Message-Id: {DA575BE5-B9F3-4E91-A40A-DCF0382F3C89-at-epfl.ch}
9, 17 -- From: Knott Graham {graham.knott-at-epfl.ch}
9, 17 -- To: Microscopy-at-microscopy.com
9, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 17 -- Content-Transfer-Encoding: 7bit
9, 17 -- Mime-Version: 1.0 (Apple Message framework v935.3)
9, 17 -- Subject: re: Cryo equipment
9, 17 -- Date: Wed, 26 Aug 2009 08:48:38 +0200
9, 17 -- X-Mailer: Apple Mail (2.935.3)
==============================End of - Headers==============================




From: dani_pss-at-hotmail.com
Date: Wed, 26 Aug 2009 06:02:30 -0500
Subject: [Microscopy] viaWWW: Doubt about cryo-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dani_pss-at-hotmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dani_pss-at-hotmail.com
Name: Daniela Silva

Organization: CEMUP

Title-Subject: [Filtered] Doubt about cryo-SEM

Question: Hello!

I have a question about cryo-SEM (I work with a crio-SEM system)and I
would like that someone could help me with this: instead of using
samples with water is it possible to use other solvents as, for
example, ethanol?

Thank you in advance.


Daniela (answer to dani_pss-at-hotmail.com)

Login Host: 193.137.26.33
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Aug 26 06:02:30 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QB2UmY018139
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 06:02:30 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c6bac5b9fe99-at-[206.69.208.22]}
10, 11 -- Date: Wed, 26 Aug 2009 06:02:29 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: dani_pss-at-hotmail.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Doubt about cryo-SEM
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dcristofori-at-unive.it
Date: Wed, 26 Aug 2009 07:23:49 -0500
Subject: [Microscopy] SEM condenser pole pieces cleaining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
a question about SEM column cleaning.
How should I clean the condenser pole pieces? For other components like
wehnelt cap, anode, etc., I use abravise paste for metals and acetone to
wash it, but I'm not sure if this is treatement might be too strong for
pole pieces.
Thanks,

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive.it

Universita' Ca' Foscari Venezia
Dipartimento di Chimica Fisica
Lab. di Scienza e Tecnologia dei Materiali
Via Torino, 155b
I-30172 Mestre (VE)
Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~


==============================Original Headers==============================
7, 26 -- From dcristofori-at-unive.it Wed Aug 26 07:23:45 2009
7, 26 -- Received: from zeus.unive.it (zeus.unive.it [157.138.1.81])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QCNgGf002906
7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 07:23:44 -0500
7, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 26 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n7QCNfeF032123
7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:23:41 +0200
7, 26 -- Received: from zeus.unive.it ([127.0.0.1])
7, 26 -- by localhost (zeus.unive.it [127.0.0.1]) (amavisd-new, port 10024)
7, 26 -- with LMTP id c8-WYmrqYduL for {microscopy-at-microscopy.com} ;
7, 26 -- Wed, 26 Aug 2009 14:23:41 +0200 (CEST)
7, 26 -- Received: from helios.unive.it (helios.unive.it [157.138.8.4])
7, 26 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n7QCNYvU032058
7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:23:34 +0200
7, 26 -- Received: from [127.0.0.1] (ferroni.dcf.unive.it [157.138.23.68])
7, 26 -- by helios.unive.it (8.13.8/8.13.8) with ESMTP id n7QCO49J005248
7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:24:09 +0200
7, 26 -- Message-ID: {4A952BF2.9010909-at-unive.it}
7, 26 -- Date: Wed, 26 Aug 2009 14:34:58 +0200
7, 26 -- From: Davide Cristofori {dcristofori-at-unive.it}
7, 26 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
7, 26 -- MIME-Version: 1.0
7, 26 -- To: microscopy-at-microscopy.com
7, 26 -- Subject: SEM condenser pole pieces cleaining
7, 26 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 26 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Wed, 26 Aug 2009 08:38:48 -0500
Subject: [Microscopy] Re: SEM condenser pole pieces cleaining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Davide et al

Several years ago someone told me they cleaned their Wehnelt and its
aperture by sonicating in Sparkleen (glassware detergent from Fisher)(or
its equivalent). I actually thought this was stupid until I tried it.
It worked very well. I've always left the pole pieces to the field
engineers, but if I were to touch one, that would be the only way I
would try to clean it.

Paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Aug 26 08:38:48 2009
6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QDckAV019862
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 08:38:47 -0500
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 -- (authenticated bits=0)
6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n7QDcg8W009869;
6, 20 -- Wed, 26 Aug 2009 08:38:43 -0500 (CDT)
6, 20 -- Message-ID: {4A953AE6.4010408-at-umanitoba.ca}
6, 20 -- Date: Wed, 26 Aug 2009 08:38:46 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: dcristofori-at-unive.it, Microscopy Listserver {microscopy-at-microscopy.com}
6, 20 -- Subject: Re: [Microscopy] SEM condenser pole pieces cleaining
6, 20 -- References: {200908261228.n7QCSuPR005793-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200908261228.n7QCSuPR005793-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Wed, 26 Aug 2009 09:10:17 -0500
Subject: [Microscopy] Re: SEM condenser pole pieces cleaining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I service technician from Zeiss told me NEVER to sonicate pure iron pole
pieces, so I never did this.

From another technician I learned this:
1- depending how dirty the surface is: cotton swaps and diamond polish
with 0,25 micron grain size; no polish like Wenol or normal household
metal ploish please...
2- brake cleaner
3- soap and water
4- aceton or ethanol some times
5- drying at 60 degrees C
It works well with all the inliner / aperture surfaces in my LaB6 SEM...
In my older Cambridge times, when I had to clean pure iron pole pieces a
lot, I used this also.

Best regards,
Stefan





dcristofori-at-unive.it schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
} a question about SEM column cleaning.
} How should I clean the condenser pole pieces? For other components like
} wehnelt cap, anode, etc., I use abravise paste for metals and acetone to
} wash it, but I'm not sure if this is treatement might be too strong for
} pole pieces.
} Thanks,
}
} Davide
}
}
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive.it
}
} Universita' Ca' Foscari Venezia
} Dipartimento di Chimica Fisica
} Lab. di Scienza e Tecnologia dei Materiali
} Via Torino, 155b
} I-30172 Mestre (VE)
} Italy
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
}
}
} ==============================Original Headers==============================
} 7, 26 -- From dcristofori-at-unive.it Wed Aug 26 07:23:45 2009
} 7, 26 -- Received: from zeus.unive.it (zeus.unive.it [157.138.1.81])
} 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QCNgGf002906
} 7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 07:23:44 -0500
} 7, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1])
} 7, 26 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n7QCNfeF032123
} 7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:23:41 +0200
} 7, 26 -- Received: from zeus.unive.it ([127.0.0.1])
} 7, 26 -- by localhost (zeus.unive.it [127.0.0.1]) (amavisd-new, port 10024)
} 7, 26 -- with LMTP id c8-WYmrqYduL for {microscopy-at-microscopy.com} ;
} 7, 26 -- Wed, 26 Aug 2009 14:23:41 +0200 (CEST)
} 7, 26 -- Received: from helios.unive.it (helios.unive.it [157.138.8.4])
} 7, 26 -- by zeus.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n7QCNYvU032058
} 7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:23:34 +0200
} 7, 26 -- Received: from [127.0.0.1] (ferroni.dcf.unive.it [157.138.23.68])
} 7, 26 -- by helios.unive.it (8.13.8/8.13.8) with ESMTP id n7QCO49J005248
} 7, 26 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 14:24:09 +0200
} 7, 26 -- Message-ID: {4A952BF2.9010909-at-unive.it}
} 7, 26 -- Date: Wed, 26 Aug 2009 14:34:58 +0200
} 7, 26 -- From: Davide Cristofori {dcristofori-at-unive.it}
} 7, 26 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302)
} 7, 26 -- MIME-Version: 1.0
} 7, 26 -- To: microscopy-at-microscopy.com
} 7, 26 -- Subject: SEM condenser pole pieces cleaining
} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
} 7, 26 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================
}

--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
10, 22 -- From stefan.diller-at-t-online.de Wed Aug 26 09:10:16 2009
10, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QEAFsu002404
10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 09:10:15 -0500
10, 22 -- Received: from fwd08.aul.t-online.de
10, 22 -- by mailout02.t-online.de with smtp
10, 22 -- id 1MgJCX-0004pS-06; Wed, 26 Aug 2009 16:10:13 +0200
10, 22 -- Received: from [192.168.2.101] (SrUYh6Zv8hB76DVrEY-Sx8rcbUCQlFrsTR9pe-nNgVju2I+WLPK75RJ0Oq0DxZpwBb-at-[93.222.96.32]) by fwd08.aul.t-online.de
10, 22 -- with esmtp id 1MgJCL-1LIRWq0; Wed, 26 Aug 2009 16:10:01 +0200
10, 22 -- Message-ID: {4A954239.1050301-at-t-online.de}
10, 22 -- Date: Wed, 26 Aug 2009 16:10:01 +0200
10, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
10, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
10, 22 -- MIME-Version: 1.0
10, 22 -- To: microscopy-at-microscopy.com
10, 22 -- Subject: Re: [Microscopy] SEM condenser pole pieces cleaining
10, 22 -- References: {200908261235.n7QCZfHO010063-at-ns.microscopy.com}
10, 22 -- In-Reply-To: {200908261235.n7QCZfHO010063-at-ns.microscopy.com}
10, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
10, 22 -- Content-Transfer-Encoding: 7bit
10, 22 -- X-ID: SrUYh6Zv8hB76DVrEY-Sx8rcbUCQlFrsTR9pe-nNgVju2I+WLPK75RJ0Oq0DxZpwBb
10, 22 -- X-TOI-MSGID: d27674cb-7677-4e4d-9c22-df17c183ba62
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Wed, 26 Aug 2009 09:22:52 -0500
Subject: [Microscopy] SEM condenser pole pieces cleaining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan et al

Stefan makes a good point that I had not thought about, that is the
composition of the part to be cleaned. Depending on surface finish,
sonication may be very bad, and that could well be for pole pieces,
whose finish and integrity is so important. As said, I have always left
that to the service people.

We have an FEI service engineer coming in over the next two weeks, I
will pick his brain before I say anymore on whether pole pieces can
safely be cleaned with sonication, and what he recommends.

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 21 -- From paul_hazelton-at-umanitoba.ca Wed Aug 26 09:22:52 2009
6, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QEMo5c016944
6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 09:22:51 -0500
6, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 21 -- (authenticated bits=0)
6, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n7QEMmOP013394;
6, 21 -- Wed, 26 Aug 2009 09:22:48 -0500 (CDT)
6, 21 -- Message-ID: {4A954539.20205-at-umanitoba.ca}
6, 21 -- Date: Wed, 26 Aug 2009 09:22:49 -0500
6, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 21 -- MIME-Version: 1.0
6, 21 -- To: stefan.diller-at-t-online.de,
6, 21 -- Microscopy Listserver {microscopy-at-microscopy.com}
6, 21 -- Subject: Re: [Microscopy] Re: SEM condenser pole pieces cleaining
6, 21 -- References: {200908261412.n7QECR5r005145-at-ns.microscopy.com}
6, 21 -- In-Reply-To: {200908261412.n7QECR5r005145-at-ns.microscopy.com}
6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: DAVE.ROSSO-at-aei.com
Date: Wed, 26 Aug 2009 10:17:03 -0500
Subject: [Microscopy] SEM - need help finding local service contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

Does anyone know of a company local to the Denver or Northern Colorado area that can service our Hitachi S-2700? The only local company I know of is Technical Sales Solutions. I would like to get quotes from a couple of different companies and pick which is best or cheapest. I have already asked for quotes from Hitachi and they are far too expensive although I'm sure they do a great job. Thanks for any and all help!

Dave

This message, including any attachments, may contain
information that is confidential and proprietary information
of Advanced Energy Industries, Inc. The dissemination,
distribution, use or copying of this message or any of its
attachments is strictly prohibited without the express
written consent of Advanced Energy Industries, Inc.


==============================Original Headers==============================
5, 33 -- From DAVE.ROSSO-at-aei.com Wed Aug 26 10:17:02 2009
5, 33 -- Received: from bsf.aei.com (bsf.aei.com [69.48.224.251])
5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7QFH0fd032601
5, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 10:17:02 -0500
5, 33 -- X-ASG-Debug-ID: 1251299818-6941010c0000-1DjkGe
5, 33 -- X-Barracuda-URL: http://bsf.aei.com:8000/cgi-bin/mark.cgi
5, 33 -- Received: from webmail.aei.com (localhost [127.0.0.1])
5, 33 -- by bsf.aei.com (Spam & Virus Firewall) with ESMTP id A53FD59F9DB
5, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 09:16:58 -0600 (MDT)
5, 33 -- Received: from webmail.aei.com ([69.48.224.228]) by bsf.aei.com with ESMTP id FZeitk7g7okCVgaj for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 09:16:58 -0600 (MDT)
5, 33 -- X-Barracuda-Envelope-From: DAVE.ROSSO-at-aei.com
5, 33 -- Received: from AEDCEXCVS0.aei.com ([172.20.30.52]) by aedcexc07.aei.com
5, 33 -- ([172.21.25.16]) with mapi; Wed, 26 Aug 2009 09:16:58 -0600
5, 33 -- From: "Rosso, Dave" {DAVE.ROSSO-at-aei.com}
5, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
5, 33 -- Date: Wed, 26 Aug 2009 09:16:56 -0600
5, 33 -- X-ASG-Orig-Subj: SEM - need help finding local service contract
5, 33 -- Subject: SEM - need help finding local service contract
5, 33 -- Thread-Topic: SEM - need help finding local service contract
5, 33 -- Thread-Index: AcomYD+qy1c52Sv9TXOhJfSwpkNBFQ==
5, 33 -- Message-ID: {78A3F1D7D37EC44CA889505ACB905BFB08852F6789-at-AEDCEXCVS0.aei.com}
5, 33 -- Accept-Language: en-US
5, 33 -- Content-Language: en-US
5, 33 -- X-MS-Has-Attach:
5, 33 -- X-MS-TNEF-Correlator:
5, 33 -- acceptlanguage: en-US
5, 33 -- Content-Type: text/plain; charset="us-ascii"
5, 33 -- MIME-Version: 1.0
5, 33 -- X-Barracuda-Connect: UNKNOWN[69.48.224.228]
5, 33 -- X-Barracuda-Start-Time: 1251299818
5, 33 -- X-Barracuda-Virus-Scanned: by Barracuda Spam & Virus Firewall at aei.com
5, 33 -- Content-Transfer-Encoding: 8bit
5, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7QFH0fd032601
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Wed, 26 Aug 2009 11:51:14 -0500
Subject: [Microscopy] New DVDs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

The main consideration when cleaning pole pieces is "Will I remove any
metal?" Any technique that removes metal, no matter how careful you are,
will eventually spoil the pole piece by changing its shape.

I feel pole pieces that have individual poles could be washed in a solvent
in an ultra sonic cleaner. I fail to understand how vibration will alter
the pole piece shape if cleaned individually? However pole pieces where the
two poles are fixed together (often soldered) should never be placed in an
ultrasonic cleaner; the vibration may crack the solder interface!

I had client who used a dental drill with a polishing head to clean the
mouth of the cathode (cathode aperture) for 15 years. They manufacturers
service technician noticed that he could not correct for condenser
astigmatism, even after twice cleaning the condenser system. I became
involved when they asked for my advice and I suggested the technician take a
good look at the cathode? Sure enough the aperture was an ellipse!

I was also brought in on an objective lens astigmatism problem where once
again the manufacturer's technician had tried everything he could think of.
The lens was water cooled with water that was too cold and condensation had
attacked the body of the lens producing permanent astigmatism, too great to
compensate. A warning to all that the water flow is for the situation when
the lens is on (100kV typically 5 amps of current being passed), but what
happens when the electronics are switched off overnight, should you switch
off?

I still marvel at the wondrous ways people use to clean cathodes. It is a
very simple task that should take about 15 minutes, the time consuming
element is often the filament alignment.

Ammonia solution (HH4OH) at a concentration in excess of 25% will clean a
cathode in an ultrasonic cleaner in about 10 minutes in most cases; ammonia
being a solvent for tungsten. Wash away the solution and rinse for 30
seconds in alcohol, dry as rapidly as possible and check with a hand lens.

Some thoughts for you.

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {paul_hazelton-at-umanitoba.ca}
To: {protrain-at-emcourses.com}
Sent: Wednesday, August 26, 2009 3:23 PM

DVD recordings of tutorials from M&M 2009 are now available.
Unfortunately, the audio was seriously compromised by unknown
factors. We apologize for this loss. Considering that the
recordings might still be of some use, since the video, and
portions of the audio, are good, we are offering them at no
charge.. Those interested in earlier tutorials should see the MSA
web page at this url: http://microscopy.org/education/library.cfm

Email is the best method for ordering. Please order by number.
We accept cash, checks in US currency, credit cards and
institutional purchase orders.

Place orders to gwe-at-ufl.edu.
Greg Erdos
5410 SE 185th Ave,
Micanopy, FL 32667

The following are the new titles.
# 315 Image processing and interpretation in structural electron
microscopy
Bernard Heymann
#316 Back to the Basics: The fundamentals of cryo-electron
microscopy
Terje Dokland
# 317 Sample Preparation for Materials Scientists
Hendrik O. Colijn

#318 Back to the Basics: The Use of Transmission Electron
Microscopy - What Are the Real Capabilities and When do I Use It?
# 319 Back to the Basics: FIB sample preparation of thin films
and soft materials
Andrew Minor
#320 Professional Development Tutorial: Career Development for
Scientific Professionals
Lucille Giannuzzi


--
Greg Erdos
Assistant Director Emeritus
Micanopy FL


==============================Original Headers==============================
8, 22 -- From gwe-at-ufl.edu Wed Aug 26 11:51:14 2009
8, 22 -- Received: from smtp.ufl.edu (smtp04.osg.ufl.edu [128.227.74.71])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QGpDRI031872
8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 11:51:14 -0500
8, 22 -- Received: from osgjas02.cns.ufl.edu (osgjas02.cns.ufl.edu [128.227.74.132])
8, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id n7QGpBXM025688
8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 12:51:11 -0400
8, 22 -- Message-ID: {60756789.113031251305471016.JavaMail.osg-at-osgjas02.cns.ufl.edu}
8, 22 -- Date: Wed, 26 Aug 2009 12:51:11 -0400 (EDT)
8, 22 -- From: greg erdos {gwe-at-ufl.edu}
8, 22 -- Reply-To: gwe-at-ufl.edu
8, 22 -- To: microscopy-at-microscopy.com
8, 22 -- Subject: New DVDs
8, 22 -- MIME-Version: 1.0
8, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
8, 22 -- Content-Transfer-Encoding: 7bit
8, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
8, 22 -- X-Originating-IP: 74.178.48.60 [74.178.48.60]
8, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.8161:2.4.5,1.2.40,4.0.166 definitions=2009-08-26_08:2009-08-11,2009-08-26,2009-08-26 signatures=0
8, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0907200000 definitions=main-0908260123
8, 22 -- X-Spam-Level: *
8, 22 -- X-UFL-Spam-Level: *
==============================End of - Headers==============================




From: DAVE.ROSSO-at-aei.com
Date: Wed, 26 Aug 2009 12:40:52 -0500
Subject: [Microscopy] Re: SEM - need help finding local service contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

Thank you for the responses online and offline.

Gary,

We are not currently experiencing any major issues but I believe the instrument is due for a good cleaning and inspection. I seem to rarely have the time to perform this myself and would feel better if someone more experienced were doing it so that we can avoid some unexpected down time. I do have an issue with what I think is vibration distortion and would like to get an opinion on what I can do to fix it (if anything).

Our 2700 also has a Lab6, Robinson BSE and an EVEX EDS (SiLi) with an EVEX digital acquisition system. When we originally bought the system it had the Quartz PCI interface. Even though it was called the PCI interface the card it used was ISA and when the PC finally died we used the opportunity to justify the new EVEX acquisition system. I really like the EVEX system as it ties the EDS in with the digital imaging system and makes it almost like a brand new digital scope.

As far as the 2700 in general I have found it to be a great scope that is easy to use and very dependable (even with limited servicing). We had a transistor fail in one of the horizontal scan outputs but other than that it has worked very well since we acquired it in 2004. If you want to know any more feel free to contact me offline as well, I would be happy to help.

Dave

This message, including any attachments, may contain
information that is confidential and proprietary information
of Advanced Energy Industries, Inc. The dissemination,
distribution, use or copying of this message or any of its
attachments is strictly prohibited without the express
written consent of Advanced Energy Industries, Inc.


==============================Original Headers==============================
9, 42 -- From DAVE.ROSSO-at-aei.com Wed Aug 26 12:40:52 2009
9, 42 -- Received: from bsf.aei.com (bsf.aei.com [69.48.224.251])
9, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7QHeqou016311
9, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 12:40:52 -0500
9, 42 -- X-ASG-Debug-ID: 1251308451-693b02f70000-1DjkGe
9, 42 -- X-Barracuda-URL: http://bsf.aei.com:8000/cgi-bin/mark.cgi
9, 42 -- Received: from webmail.aei.com (localhost [127.0.0.1])
9, 42 -- by bsf.aei.com (Spam & Virus Firewall) with ESMTP id 9ED94C49B7
9, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 11:40:51 -0600 (MDT)
9, 42 -- Received: from webmail.aei.com ([69.48.224.228]) by bsf.aei.com with ESMTP id dbjfFs9o9Ii2EeBJ for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 11:40:51 -0600 (MDT)
9, 42 -- X-Barracuda-Envelope-From: DAVE.ROSSO-at-aei.com
9, 42 -- Received: from AEDCEXC02.aei.com (172.20.30.33) by AEDCEXC08.aei.com
9, 42 -- (172.21.25.17) with Microsoft SMTP Server (TLS) id 8.1.340.0; Wed, 26 Aug
9, 42 -- 2009 11:40:51 -0600
9, 42 -- Received: from AEDCEXCVS0.aei.com ([172.20.30.52]) by AEDCEXC02.aei.com
9, 42 -- ([172.20.30.33]) with mapi; Wed, 26 Aug 2009 11:40:51 -0600
9, 42 -- From: "Rosso, Dave" {DAVE.ROSSO-at-aei.com}
9, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
9, 42 -- Date: Wed, 26 Aug 2009 11:40:47 -0600
9, 42 -- X-ASG-Orig-Subj: Re: [Microscopy] SEM - need help finding local service contract
9, 42 -- Subject: Re: [Microscopy] SEM - need help finding local service contract
9, 42 -- Thread-Topic: Re: [Microscopy] SEM - need help finding local service contract
9, 42 -- Thread-Index: AcomdFg8BD3LxoYMQ5iIg5yUvx/nCA==
9, 42 -- Message-ID: {78A3F1D7D37EC44CA889505ACB905BFB08852F6997-at-AEDCEXCVS0.aei.com}
9, 42 -- Accept-Language: en-US
9, 42 -- Content-Language: en-US
9, 42 -- X-MS-Has-Attach:
9, 42 -- X-MS-TNEF-Correlator:
9, 42 -- x-cr-hashedpuzzle: Ag4q Ate1 Brpf BzxJ EN/a EqcL E7I9 G6ma Hs/H H678 IGiX
9, 42 -- IUo+ IhOh JE7x Kprt
9, 42 -- LX5y;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{3E3E4C5C-B598-4630-9FA1-96F03874C8BA};ZABhAHYAZQAuAHIAbwBzAHMAbwBAAGEAZQBpAC4AYwBvAG0A;Wed,
9, 42 -- 26 Aug 2009 17:40:47
9, 42 -- GMT;UgBlADoAIABbAE0AaQBjAHIAbwBzAGMAbwBwAHkAXQAgAFMARQBNACAALQAgAG4AZQBlAGQAIABoAGUAbABwACAAZgBpAG4AZABpAG4AZwAgAGwAbwBjAGEAbAAgAHMAZQByAHYAaQBjAGUAIABjAG8AbgB0AHIAYQBjAHQA
9, 42 -- x-cr-puzzleid: {3E3E4C5C-B598-4630-9FA1-96F03874C8BA}
9, 42 -- acceptlanguage: en-US
9, 42 -- Content-Type: text/plain; charset="us-ascii"
9, 42 -- MIME-Version: 1.0
9, 42 -- X-Barracuda-Connect: UNKNOWN[69.48.224.228]
9, 42 -- X-Barracuda-Start-Time: 1251308451
9, 42 -- X-Barracuda-Virus-Scanned: by Barracuda Spam & Virus Firewall at aei.com
9, 42 -- Content-Transfer-Encoding: 8bit
9, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7QHeqou016311
==============================End of - Headers==============================




From: harvard.armus-at-utoledo.edu
Date: Wed, 26 Aug 2009 18:38:09 -0500
Subject: [Microscopy] viaWWW: microscope light bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both harvard.armus-at-utoledo.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: harvard.armus-at-utoledo.edu
Name: Harvard Armus

Organization: University of Toledo

Title-Subject: [Filtered] microscope light bulb

Question: For my work on learning in paramecia I have been using an
old, but fine, Leitz projection microscope. Unfortunately, the last
bulb has died, and, as this bulb is no longer made, there is a bit of
a problem. The bulb is a Philips metal halide 250w lamp (CSI 250w/4).
On the off chance that anyone has one of these lying around, I'd do
almost anything halfway reasonable (or even not so reasonable) to get
it.
Harvey Armus

Login Host: 131.183.80.171
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Aug 26 18:38:09 2009
6, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QNc7Vs011933
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 18:38:08 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c6bb77c4b9e4-at-[206.69.208.22]}
6, 11 -- Date: Wed, 26 Aug 2009 18:38:06 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: harvard.armus-at-utoledo.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: microscope light bulb
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ann.lehman-at-trincoll.edu
Date: Wed, 26 Aug 2009 18:38:36 -0500
Subject: [Microscopy] viaWWW: LogEtronics Enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ann.lehman-at-trincoll.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ann.lehman-at-trincoll.edu
Name: Ann Lehman

Organization: Trinity College EM Facility, Hartford CT

Title-Subject: [Filtered] LogEtronics Enlarger

Question: Dear Listers,

I'm closing down a darkroom and have a large format LogE-55 enlarger
by LogEtronics available if anyone can use it. Hate to put it out on
the dump heap but that is where it is headed in a couple of weeks.

Ann Lehman
EM Facility at Trinity College
office 860-297-4289
cell 203-768-5598

Login Host: 157.252.98.224
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Aug 26 18:38:36 2009
8, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7QNcYLh012301
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 18:38:35 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240802c6bb77e8c27a-at-[206.69.208.22]}
8, 11 -- Date: Wed, 26 Aug 2009 18:38:34 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: ann.lehman-at-trincoll.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: LogEtronics Enlarger
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 26 Aug 2009 18:49:08 -0500
Subject: [Microscopy] Re: viaWWW: microscope light bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try a Google for

philips CSI 250w/4

and see if any of the hits are what you seek.
Looks like most sources are in UK.

gary g.

At 02:44 PM 8/26/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Wed Aug 26 18:49:08 2009
9, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7QNn7Wm003074
9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 18:49:07 -0500
9, 20 -- Message-Id: {200908262349.n7QNn7Wm003074-at-ns.microscopy.com}
9, 20 -- Received: (qmail 11123 invoked from network); 26 Aug 2009 17:16:57 -0700
9, 20 -- Received: by simscan 1.1.0 ppid: 11116, pid: 11118, t: 0.1061s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp1 with SMTP; 26 Aug 2009 17:16:57 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Wed, 26 Aug 2009 16:49:02 -0700
9, 20 -- To: harvard.armus-at-utoledo.edu
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: microscope light bulb
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200908262340.n7QNeKii016519-at-ns.microscopy.com}
9, 20 -- References: {200908262340.n7QNeKii016519-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Wed, 26 Aug 2009 18:57:28 -0500
Subject: [Microscopy] Tissue Embedding Center

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings

I want to resurrect a Reichert-Jung Tissue Embedding Center that has
been in the lab for a long time (before I got here).

No luck finding the directions, so I turn to you for help.

I have done simple plant microtechnique, but never with such a fine
machine as a Tissue Embedding Center. I know the process, but not the
specifics of this machine, like where the warm and cold spots are, and
how it is supposed to be used and cleaned.

If you have experience with something like this and could help me get
started, I would be grateful.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
10, 37 -- From jkrupp-at-deltacollege.edu Wed Aug 26 18:57:27 2009
10, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
10, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n7QNvRiL022097
10, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 18:57:27 -0500
10, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
10, 37 -- by localhost (Email Security Appliance) with SMTP id 11FAC1B93AD_A95C527B
10, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 23:28:39 +0000 (GMT)
10, 37 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
10, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 059991A14F0_A95C527F
10, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 23:28:39 +0000 (GMT)
10, 37 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
10, 37 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
10, 37 -- with ESMTP id 48923180 for microscopy-at-microscopy.com; Wed, 26 Aug 2009 16:57:25 -0700
10, 37 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by
10, 37 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
10, 37 -- ESMTP id KP0D7O00.2G0 for {microscopy-at-microscopy.com} ; Wed, 26
10, 37 -- Aug 2009 16:57:24 -0700
10, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1])
10, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5D41F7A38513
10, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 16:57:25 -0700 (PDT)
10, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu
10, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
10, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
10, 37 -- with ESMTP id Q0Z4PxDsfFgS for {microscopy-at-microscopy.com} ;
10, 37 -- Wed, 26 Aug 2009 16:57:24 -0700 (PDT)
10, 37 -- Received: from [172.20.3.214] (unknown [172.20.3.214])
10, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id EFED37A3850F
10, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 16:57:24 -0700 (PDT)
10, 37 -- Message-Id: {96B1877D-3C97-4A36-B313-EF6FD06AFE0B-at-deltacollege.edu}
10, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
10, 37 -- To: microscopy-at-microscopy.com
10, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 37 -- Content-Transfer-Encoding: 7bit
10, 37 -- Mime-Version: 1.0 (Apple Message framework v936)
10, 37 -- Subject: Tissue Embedding Center
10, 37 -- Date: Wed, 26 Aug 2009 16:57:24 -0700
10, 37 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: knick-at-llu.edu
Date: Wed, 26 Aug 2009 23:08:58 -0500
Subject: [Microscopy] SEM - Advice on sputter coater model

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all:

I am planning to buy a new sputter coater that will be used to primarily to prepare rock samples for SEM analysis. I have used Hummer and Cressington coaters, but I see that there are many other models to choose from: Denton, Advanced Process Technology, ScanCoat, and more.

Looking for advice/opinions on:
1) is there a clear leader for our application?
2) do combination metal/carbon systems work for an operation that mostly will coat with metal and occasionally coat with carbon?

Thank you,

Kevin Nick

Dept. of Earth and Biological Sciences
Loma Linda University, CA


==============================Original Headers==============================
8, 20 -- From knick-at-llu.edu Wed Aug 26 23:08:58 2009
8, 20 -- Received: from oakmont.llu.ad.lluahsc.org (oakmont.llu.ad.lluahsc.org [151.112.1.16])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7R48vu0011846
8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 23:08:57 -0500
8, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 20 -- Content-class: urn:content-classes:message
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: text/plain;
8, 20 -- charset="iso-8859-1"
8, 20 -- Subject: SEM - Advice on sputter coater model
8, 20 -- Date: Wed, 26 Aug 2009 21:08:57 -0700
8, 20 -- Message-ID: {6B9B0CEF7F409D439E78288D8665BD8F3603A0-at-oakmont.llu.ad.lluahsc.org}
8, 20 -- X-MS-Has-Attach:
8, 20 -- X-MS-TNEF-Correlator:
8, 20 -- Thread-Topic: SEM - Advice on sputter coater model
8, 20 -- Thread-Index: AcomzBkGMWl91zeVRlKPTJSbprWu2Q==
8, 20 -- From: "Nick, Kevin (LLU)" {knick-at-llu.edu}
8, 20 -- To: {Microscopy-at-microscopy.com}
8, 20 -- Content-Transfer-Encoding: 8bit
8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n7R48vu0011846
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 27 Aug 2009 08:38:34 -0500
Subject: [Microscopy] Clostridium difficile

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I am looking for a SEM and / or TEM specimen of Clostridium difficile.
Anybody out there who is willing to share a good specimen. I will send
it back after some days of work on it and cover your expenses.

Thanks,
Stefan


--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
5, 20 -- From stefan.diller-at-t-online.de Thu Aug 27 08:38:34 2009
5, 20 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7RDcWCK017203
5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2009 08:38:33 -0500
5, 20 -- Received: from fwd03.aul.t-online.de
5, 20 -- by mailout02.t-online.de with smtp
5, 20 -- id 1MgfBQ-0007lx-01; Thu, 27 Aug 2009 15:38:32 +0200
5, 20 -- Received: from [192.168.2.101] (ZZzrL2ZUZh1BP6ftDDj5AeUegIl2kWCNVsDKJwfWLNL95El0UrmYS3SUE8v0ERTgsm-at-[93.222.104.145]) by fwd03.aul.t-online.de
5, 20 -- with esmtp id 1MgfBH-1VsHAm0; Thu, 27 Aug 2009 15:38:23 +0200
5, 20 -- Message-ID: {4A968C4F.6000400-at-t-online.de}
5, 20 -- Date: Thu, 27 Aug 2009 15:38:23 +0200
5, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
5, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
5, 20 -- MIME-Version: 1.0
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- Subject: Clostridium difficile
5, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
5, 20 -- Content-Transfer-Encoding: 7bit
5, 20 -- X-ID: ZZzrL2ZUZh1BP6ftDDj5AeUegIl2kWCNVsDKJwfWLNL95El0UrmYS3SUE8v0ERTgsm
5, 20 -- X-TOI-MSGID: a123dbd1-eb7f-4141-9b70-c10a33e582d8
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Thu, 27 Aug 2009 09:06:05 -0500
Subject: [Microscopy] Re: New DVDs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Greg,
It's so good of you to keep and promote the 314 instructional DVDs but
having the list of titles as a PDF that is not organized by subject
matter or in alphabetical order is truly not user friendly. When is
the society going to spend the money to create a searchable data base
of the DVDs? That will be a useful change.

Beth

On Aug 26, 2009, at 12:52 PM, gwe-at-ufl.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} DVD recordings of tutorials from M&M 2009 are now available.
} Unfortunately, the audio was seriously compromised by unknown
} factors. We apologize for this loss. Considering that the
} recordings might still be of some use, since the video, and
} portions of the audio, are good, we are offering them at no
} charge.. Those interested in earlier tutorials should see the MSA
} web page at this url: http://microscopy.org/education/library.cfm
}
} Email is the best method for ordering. Please order by number.
} We accept cash, checks in US currency, credit cards and
} institutional purchase orders.
}
} Place orders to gwe-at-ufl.edu.
} Greg Erdos
} 5410 SE 185th Ave,
} Micanopy, FL 32667
}
} The following are the new titles.
} # 315 Image processing and interpretation in structural electron
} microscopy
} Bernard Heymann
} #316 Back to the Basics: The fundamentals of cryo-electron
} microscopy
} Terje Dokland
} # 317 Sample Preparation for Materials Scientists
} Hendrik O. Colijn
}
} #318 Back to the Basics: The Use of Transmission Electron
} Microscopy - What Are the Real Capabilities and When do I Use It?
} # 319 Back to the Basics: FIB sample preparation of thin films
} and soft materials
} Andrew Minor
} #320 Professional Development Tutorial: Career Development for
} Scientific Professionals
} Lucille Giannuzzi
}
}
} --
} Greg Erdos
} Assistant Director Emeritus
} Micanopy FL
}
}
} ==============================Original
} Headers==============================
} 8, 22 -- From gwe-at-ufl.edu Wed Aug 26 11:51:14 2009
} 8, 22 -- Received: from smtp.ufl.edu (smtp04.osg.ufl.edu
} [128.227.74.71])
} 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n7QGpDRI031872
} 8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 11:51:14
} -0500
} 8, 22 -- Received: from osgjas02.cns.ufl.edu (osgjas02.cns.ufl.edu
} [128.227.74.132])
} 8, 22 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id
} n7QGpBXM025688
} 8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2009 12:51:11
} -0400
} 8, 22 -- Message-ID: {60756789.113031251305471016.JavaMail.osg-at-osgjas02.cns.ufl.edu
} }
} 8, 22 -- Date: Wed, 26 Aug 2009 12:51:11 -0400 (EDT)
} 8, 22 -- From: greg erdos {gwe-at-ufl.edu}
} 8, 22 -- Reply-To: gwe-at-ufl.edu
} 8, 22 -- To: microscopy-at-microscopy.com
} 8, 22 -- Subject: New DVDs
} 8, 22 -- MIME-Version: 1.0
} 8, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii
} 8, 22 -- Content-Transfer-Encoding: 7bit
} 8, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
} 8, 22 -- X-Originating-IP: 74.178.48.60 [74.178.48.60]
} 8, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure
} engine=1.12.8161:2.4.5,1.2.40,4.0.166
} definitions=2009-08-26_08:2009-08-11,2009-08-26,2009-08-26
} signatures=0
} 8, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default
} score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0
} classifier=spam adjust=0 reason=mlx engine=5.0.0-0907200000
} definitions=main-0908260123
} 8, 22 -- X-Spam-Level: *
} 8, 22 -- X-UFL-Spam-Level: *
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
6, 21 -- From beth-at-plantbio.uga.edu Thu Aug 27 09:06:01 2009
6, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7RE5xwm032722
6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2009 09:06:00 -0500
6, 21 -- Received: from [128.192.26.46] ([128.192.26.46])
6, 21 -- (authenticated user beth-at-plantbio.uga.edu)
6, 21 -- by dogwood.plantbio.uga.edu
6, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
6, 21 -- for microscopy-at-microscopy.com;
6, 21 -- Thu, 27 Aug 2009 10:05:25 -0400
6, 21 -- Message-Id: {3E2AC0AC-81C9-4CDC-B36F-80D3CCC18001-at-plantbio.uga.edu}
6, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
6, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com}
6, 21 -- In-Reply-To: {200908261652.n7QGq0Kr000364-at-ns.microscopy.com}
6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- Mime-Version: 1.0 (Apple Message framework v930.3)
6, 21 -- Subject: Re: [Microscopy] New DVDs
6, 21 -- Date: Thu, 27 Aug 2009 10:05:26 -0400
6, 21 -- References: {200908261652.n7QGq0Kr000364-at-ns.microscopy.com}
6, 21 -- X-Mailer: Apple Mail (2.930.3)
==============================End of - Headers==============================




From: ehuber-at-nature.berkeley.edu
Date: Thu, 27 Aug 2009 14:30:43 -0500
Subject: [Microscopy] Advice needed for using ImageJ to measure ring spacing of fish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Please let me briefly introduce myself. My name is Eric Huber and I’m a
fisheries researcher at the University of California, Berkeley. I would
like to measure the ring spacing of fish scales and otoliths with Image J
(same principles of tree ring analysis apply). In the past I have used
Optimas (now ImagePro) software to measure the ring spacing of otoliths.
For that I was able to run a transect from the core of the otolith out to
the edge and place hash marks at each ring. Optimas would then provide me
with data on the distance between each ring. I have yet to be able to
figure out a way to do this in Image J. It would be ideal to figure out a
way to automate the process (i.e., automatically have ImageJ place hash
marks at the rings along the transect). As you can probably tell, I am
VERY new to ImageJ and would really appreciate some feedback. Also, if you
know of any good macros or plugins out there, please let me know.

Many thanks in advance,
Eric Huber
Carlson Laboratory Manager
University of California, Berkeley
Dept. of Environmental Science, Policy & Management
140 Mulford Hall #3114
Berkeley, CA 94720
Office: 304 Mulford Hall
http://nature.berkeley.edu/~ehuber


==============================Original Headers==============================
3, 29 -- From ehuber-at-nature.berkeley.edu Thu Aug 27 14:30:42 2009
3, 29 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [169.229.201.201])
3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7RJUf3F027372
3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2009 14:30:42 -0500
3, 29 -- Received: from localhost (localhost [127.0.0.1])
3, 29 -- by nature.Berkeley.EDU (Postfix) with ESMTP id D0C114D254
3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2009 12:30:37 -0700 (PDT)
3, 29 -- Received: from nature.Berkeley.EDU ([127.0.0.1])
3, 29 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-maia, port 10024)
3, 29 -- with ESMTP id 25765-06 for {microscopy-at-microscopy.com} ;
3, 29 -- Thu, 27 Aug 2009 12:30:35 -0700 (PDT)
3, 29 -- Received: from nature.berkeley.edu (localhost [127.0.0.1])
3, 29 -- by nature.Berkeley.EDU (Postfix) with ESMTP id DF0574D252
3, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 27 Aug 2009 12:30:35 -0700 (PDT)
3, 29 -- Received: from 128.32.224.96
3, 29 -- (SquirrelMail authenticated user ehuber)
3, 29 -- by nature.berkeley.edu with HTTP;
3, 29 -- Thu, 27 Aug 2009 12:30:35 -0700
3, 29 -- Message-ID: {e4a0b3f56724d86c6225c6bff2f1d800.squirrel-at-nature.berkeley.edu}
3, 29 -- Date: Thu, 27 Aug 2009 12:30:35 -0700
3, 29 -- Subject: Advice needed for using ImageJ to measure ring spacing of fish
3, 29 -- scales
3, 29 -- From: "Eric Huber" {ehuber-at-nature.berkeley.edu}
3, 29 -- To: Microscopy-at-microscopy.com
3, 29 -- User-Agent: SquirrelMail/1.5.2 [SVN]
3, 29 -- MIME-Version: 1.0
3, 29 -- Content-Type: text/plain;charset=iso-8859-1
3, 29 -- Content-Transfer-Encoding: 8bit
3, 29 -- X-Virus-Scanned: Maia Mailguard 1.0.2
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 28 Aug 2009 05:46:49 -0500
Subject: [Microscopy] Equipment for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Due to the untimely departure of a colleague in the petrography field,
several light microscopes have become available. These include an Olympus
BHS polarizing light microscope (with a wide range of objectives,
compensators and a filar micrometer eyepiece), an Olympus CH30 setup with
phase for asbestos counting and several others.

Two Olympus stereomicroscopes (one with a boom stand) and a couple light
sources are also available.

All of the equipment has been cared for and is in good condition.
Interested individuals should e-mail my friend Jay at jcdmb-at-aol.com for
additional information.


Stay safe…….
Frank
--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
7, 23 -- From frank_karl-at-lincolnelectric.com Fri Aug 28 05:46:49 2009
7, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7SAkmDv011543
7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Aug 2009 05:46:48 -0500
7, 23 -- Subject: Equipment for sale
7, 23 -- To: Microscopy-at-microscopy.com
7, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 23 -- Message-ID: {OF61471337.B7B73450-ON85257620.003AFBBC-85257620.003B32D3-at-lincolnelectric.com}
7, 23 -- Date: Fri, 28 Aug 2009 06:46:50 -0400
7, 23 -- From: Frank_Karl-at-lincolnelectric.com
7, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 23 -- 07, 2008) at 08/28/2009 06:46:28 AM,
7, 23 -- CD-MIME complete at 08/28/2009 06:46:28 AM,
7, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 23 -- 07, 2008) at 08/28/2009 06:46:28 AM,
7, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 23 -- 07, 2008) at 08/28/2009 06:46:28 AM,
7, 23 -- Serialize complete at 08/28/2009 06:46:28 AM
7, 23 -- MIME-Version: 1.0
7, 23 -- Content-Type: text/plain;
7, 23 -- charset="UTF-8"
7, 23 -- Content-Transfer-Encoding: 8bit
7, 23 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n7SAkmDv011543
==============================End of - Headers==============================




From: jpare-at-emory.edu
Date: Fri, 28 Aug 2009 09:43:09 -0500
Subject: [Microscopy] viaWWW: Freeze fracture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jpare-at-emory.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jpare-at-emory.edu
Name: Jeff Pare

Organization: Yerkes Res. Center / Emory Univeristy

Title-Subject: [Filtered] Freeze fracture

Question: Good morning everyone,

Our laboratory would like to start using the freeze fracture method
but have no idea how to get this going; Is there anyone out there who
knows about a workshop on that very subject?

Please let me know if you know of one in the near future

Thanks

Jeff


Login Host: 170.140.166.234
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Aug 28 09:43:08 2009
11, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7SEh40e002374
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 28 Aug 2009 09:43:07 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240803c6bd9d658e4c-at-[206.69.208.22]}
11, 11 -- Date: Fri, 28 Aug 2009 09:43:02 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: jpare-at-emory.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Freeze fracture
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Mon, 31 Aug 2009 09:48:12 -0500
Subject: [Microscopy] Advanced PFM workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

As a reminder, to day is the last day to register for the CNMS User
meeting and the Advanced PFM workshop. The 2009 annual user meetings for
CNMS and SHaRE are held jointly on September 16-17, 2009, Wednesday and
Thursday, at the Oak Ridge National Laboratory in Oak Ridge, Tennessee.
The annual user meeting combines oral presentations, poster sessions,
workshops and tutorials into a compact program designed to illuminate
the frontiers of nanoscience research and acquaint researchers with the
scientific resources for nanoscience and advanced electron microscopy
that these two user facilities offer to them. The registration
information can be found on www.cnms.ornl.gov.
****
The Advanced PFM workshop will be held on September 15. The tentative
program is:

8.30 a.m. - 10.00 a.m. S.V. Kalinin, Principles and advanced dynamic
modes of PFM
10.00 - 10.30 Coffee break
10.30 - 12.00 A. Baddorf, Surface science methods and in-situ studies of
oxides
12.00 - 13.00 Lunch
13.00 - 14.00 P. Maksymovych, Transport measurements and Ferroelectric
Tunneling by PFM
14.00 - 15.00 S.V. Kalinin, Spectroscopic measurements in PFM
15.00 - 15.30 Coffee break
15.30 - 16.30. M. Nikiforov, Advanced Thermomechanical characterization
by SPM
16.30 - 17.15 N. Balke, Deterministic ferroelastic switching in
multiferroics
17.15-18.00 V. Reukov (Clemson), Biological recognition in PFM

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
7, 24 -- From sergei2-at-ornl.gov Mon Aug 31 09:48:11 2009
7, 24 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7VEmBun016771
7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 09:48:11 -0500
7, 24 -- Received: from emroute3.ornl.gov ([127.0.0.1])
7, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
7, 24 -- with ESMTP id {0KP800K0RX4AZZ-at-emroute3.ornl.gov} for
7, 24 -- microscopy-at-microscopy.com; Mon, 31 Aug 2009 10:48:10 -0400 (EDT)
7, 24 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov
7, 24 -- (PMDF V6.4 #31561) id {0KP800L01X4A5Y-at-emroute3.ornl.gov} for
7, 24 -- microscopy-at-microscopy.com; Mon, 31 Aug 2009 10:48:10 -0400 (EDT)
7, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
7, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
7, 24 -- with ESMTP id {0KP800K6ZX4AQJ-at-emroute3.ornl.gov} for
7, 24 -- microscopy-at-microscopy.com; Mon, 31 Aug 2009 10:48:10 -0400 (EDT)
7, 24 -- Date: Mon, 31 Aug 2009 10:48:11 -0400
7, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
7, 24 -- Subject: Advanced PFM workshop
7, 24 -- To: microscopy-at-microscopy.com
7, 24 -- Message-id: {4A9BE2AB.7030408-at-ornl.gov}
7, 24 -- MIME-version: 1.0
7, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
7, 24 -- Content-transfer-encoding: 7bit
7, 24 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
==============================End of - Headers==============================




From: marksmsa-at-gmail.com
Date: Mon, 31 Aug 2009 14:58:13 -0500
Subject: [Microscopy] Postdoctoral position in electron microscopy of Metal-on-Metal hip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A postdoctoral position is available at Northwestern University to
work on Metal-on-Metal total hip replacements. The project will
involve using transmission electron microscopy (ranging from
conventional bright-field/dark-field to atomic-scale chemical
analysis) to examine the wear mechanisms at the metal/metal interface
due to tribochemical reactions and surface fatigue. These
tribochemical reactions result in the reaction of the metal bearings
with the interfacial medium (e.g., synovial fluid) to form a layer
which consists of a mixture of organic, ceramic and metallic
constituents. The goal will be to determine the structure-properties
relationship (mechanically, chemically) at the nanoscale during
operation of the near-surface region of the metal, and determine the
chemical changes taking place, in addition to dislocation structure
development and grain-size, whether there is incorporation of
impurities in the metal and whether segregation to the grain
boundaries near the surface plays a role in the wear. The postdoctoral
scientist will work collaboratively with other postdocs and students
at Northwestern University who are working on more tribological
aspects of the work, as well as tribologists and surgeons at Rush
University Medical Center.

A strong background in transmission electron microscopy with good
competence in metallurgy is a requirement. Additional experience with
biological systems and tribology will be a plus, but is not a
requirement.

Applicants should send a copy of their CV and the names of three
referees to L-marks-at-northwestern.edu.


--
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60208, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L-marks at northwestern dot edu
Web: www.numis.northwestern.edu
EMM2007 http://ns.crys.ras.ru/EMMM07/
Commission on Electron Diffraction of IUCR
www.numis.northwestern.edu/IUCR_CED

==============================Original Headers==============================
5, 30 -- From marksmsa-at-gmail.com Mon Aug 31 14:58:13 2009
5, 30 -- Received: from mail-bw0-f222.google.com (mail-bw0-f222.google.com [209.85.218.222])
5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n7VJwCCW021133
5, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 14:58:13 -0500
5, 30 -- Received: by bwz22 with SMTP id 22so3108393bwz.18
5, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 12:58:10 -0700 (PDT)
5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 30 -- d=gmail.com; s=gamma;
5, 30 -- h=domainkey-signature:mime-version:received:date:message-id:subject
5, 30 -- :from:to:content-type;
5, 30 -- bh=OnnpBjevWrsJF41hqC+GkDUbXBc7vAnRw4H13SIbvRU=;
5, 30 -- b=U0nVH2x4FqTuZgYGJP7T3tU+oOuEkS6qEFq6cp7sTyFJgxJeC/A5IZRG7o4of9T8ZW
5, 30 -- 6b61alz86o66Tc+gLf/1UdlH8RYk2fDwD8iWsaL944fNVqTJT3WrcQhfkAs+XPOOGHbD
5, 30 -- foOZzQa8nP4XQMixTyUOZGF6c9KmS9jow4c8U=
5, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 30 -- d=gmail.com; s=gamma;
5, 30 -- h=mime-version:date:message-id:subject:from:to:content-type;
5, 30 -- b=H4Cxx6Me9flIIUR97bxQ6PtY8HJNqa7xDuKfkthO2/ehpdJ5qVEabWQjcdvw87Ii3E
5, 30 -- BEvXOQzfDjpi6ZJayLaczKRkLknpapW9ynmlX5TICG6qVQsxkBi97Kpt6DH0N5CrocTw
5, 30 -- MIHH+dyjsf5M6p1tW4Wr70w7tlf+fWtRaVIaY=
5, 30 -- MIME-Version: 1.0
5, 30 -- Received: by 10.239.145.11 with SMTP id q11mr451144hba.98.1251748690377; Mon,
5, 30 -- 31 Aug 2009 12:58:10 -0700 (PDT)
5, 30 -- Date: Mon, 31 Aug 2009 14:58:10 -0500
5, 30 -- Message-ID: {e13ba6260908311258l20a866hfd5afad50f63ce12-at-mail.gmail.com}
5, 30 -- Subject: Postdoctoral position in electron microscopy of Metal-on-Metal hip
5, 30 -- replacements.
5, 30 -- From: L Marks {marksmsa-at-gmail.com}
5, 30 -- To: microscopy {Microscopy-at-microscopy.com}
5, 30 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: jinsong-wu-at-northwestern.edu
Date: Mon, 31 Aug 2009 20:18:38 -0500
Subject: [Microscopy] viaWWW: Two postdoc. positions at Northwestern University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jinsong-wu-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jinsong-wu-at-northwestern.edu
Name: jinsong wu

Organization: northwestern university

Title-Subject: [Filtered] Two postdoc. positions at Northwestern University

Question: OPEN POSITIONS

Postdoctoral Scholars/Research Associates

Analytical Electron Microscopy and Tomography of Nanostructures

International Institute for Nanotechnology (IIN)

Department of Materials Science & Engineering,

Northwestern University, Evanston, IL

Two postdoctoral scholar/research associate positions are immediately
available at Northwestern University in the broad area of advanced
analytical S/TEM and 3-D tomography of nanostructured materials.

The associated research projects are being supervised by Professor
Vinayak P. Dravid, and concern with S/TEM analysis of nanoscale
structure, assembly/organization, local chemistry and electronic
structure of advanced nanostructures; ranging from thermoelectrics to
catalysts and nanopatterned structures.

The positions will make use of recently installed Hitachi dual-EDS
field emission HD-2300A STEM, FEI Helios NanoLab, field emission
JEOL-2100 FasTEM, access to aberration-corrected S/TEMs,
Local-Electrode Atom Probe (LEAP), and range of specimen holders for
analytical and 3-D S/TEM tomography; all available through the
NUCenter (www.nuance.northwestern.edu). This is a part of extensive
cross-disciplinary and collaborative activities in nanotechnology at
Northwestern. As a result, the candidate would have ample opportunity
to learn diverse aspects of nanotechnology, while contributing to
S/TEM analysis of nanostructures.

The positions require PhD in physical sciences/engineering.
Considerable hands-on experience in advanced S/TEM and techniques
such as HRTEM, 3-D tomography, HAADF, EDS/EELS, e- diffraction and
computation/simulations is required. Experience in imaging
filter/spectral imaging is highly desirable.

The positions are available immediately for at least two years with
possibility for extension for additional years upon mutual agreement.

Salary and compensation would commensurate with experience, in the
range: $35,000-$60,000

Please forward resume with at least two letters of references,
electronically, to:

Email: m-paidar-at-northwestern.edu

c/o Professor Vinayak P. Dravid

Materials Science & Engineering

Director, NUCenter

Northwestern University, Evanston, IL 60208, USA

Ph.: (847) 467-1363, Fax: (847) 491-7820

http://vpd.ms.northwestern.edu

http://www.nuance.northwestern


Login Host: 129.105.37.169
---------------------------------------------------------------------------

==============================Original Headers==============================
27, 11 -- From zaluzec-at-microscopy.com Mon Aug 31 20:18:38 2009
27, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
27, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n811IavC012774
27, 11 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 20:18:37 -0500
27, 11 -- Mime-Version: 1.0
27, 11 -- Message-Id: {p06240800c6c226c4c5cf-at-[206.69.208.22]}
27, 11 -- Date: Mon, 31 Aug 2009 20:18:36 -0500
27, 11 -- To: microscopy-at-microscopy.com
27, 11 -- From: jinsong-wu-at-northwestern.edu (by way of MicroscopyListserver)
27, 11 -- Subject: viaWWW: Two postdoc. positions at Northwestern University
27, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: modla-at-dbi.udel.edu
Date: Mon, 31 Aug 2009 20:19:06 -0500
Subject: [Microscopy] viaWWW: Tomography Resources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: modla-at-dbi.udel.edu
Name: Shannon Modla

Organization: University of Delaware

Title-Subject: [Filtered] Tomography Resources

Question: Our lab has just recently started doing tomography. We are
currently trying to learn some of the offline software like IMOD. I
was wondering if anyone knew of any courses, workshops, or other such
resources available to receive training in using IMOD.

Best regards,
Shannon

Login Host: 128.175.253.84
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Aug 31 20:19:05 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n811J4Mv013394
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 20:19:05 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c6c226f7d1e6-at-[206.69.208.22]}
7, 11 -- Date: Mon, 31 Aug 2009 20:19:03 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Tomography Resources
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: eikonika-at-otenet.gr
Date: Mon, 31 Aug 2009 23:12:48 -0500
Subject: [Microscopy] jeol sem water cooling tubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Friends
I noticed that the water tubes coming in and out of my JSM 5600 LV
microscope were twisted together in several circles. Close contact of the
two tubes results to a counter-current mechanism of heat exchange IinI {-
IoutI that reduces drastically the efficiency of cooling and increases water
flow requirements for cooling. On the other hand it prevents very cold water
from reaching the pump. This efficient mechanism is active in the
extremities of all hot-blooded animals by means of close contact between
veins and arteries.
Does anybody know:
1. Are the tubes put together on some purpose by jeol or it just happened to
my microscope (bought second hand but probably decommissioned by jeol)?
2. Can I separate the tubes and save water in my place where tap water never
has very low temperatures?
Regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477


==============================Original Headers==============================
4, 22 -- From eikonika-at-otenet.gr Mon Aug 31 23:12:47 2009
4, 22 -- Received: from kane.otenet.gr (kane.otenet.gr [83.235.67.31])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n814Ck8C015194
4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 23:12:47 -0500
4, 22 -- Received: from axeron (athedsl-322635.home.otenet.gr [85.72.115.233])
4, 22 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id n814Cjoc002490
4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 07:12:45 +0300
4, 22 -- Message-ID: {001701ca2aba$80dcfb50$1601a8c0-at-axeron}
4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
4, 22 -- To: {microscopy-at-microscopy.com}
4, 22 -- Subject: jeol sem water cooling tubes
4, 22 -- Date: Tue, 1 Sep 2009 07:12:32 +0300
4, 22 -- MIME-Version: 1.0
4, 22 -- Content-Type: text/plain;
4, 22 -- format=flowed;
4, 22 -- charset="iso-8859-7";
4, 22 -- reply-type=original
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- X-Priority: 3
4, 22 -- X-MSMail-Priority: Normal
4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 1 Sep 2009 09:39:02 -0500
Subject: [Microscopy] LM: Stain for myelin in plastic sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

Does anyone have a favorite stain for myelin in plastic thick sections?


We have been given one protocol using P-phenylene diamine in absolute
ethanol, and we will give it a try, but if there are any other pet
techniques that you would be willing to share, we would love to try
them, too.

Thanks!

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original Headers==============================
9, 27 -- From TindallR-at-missouri.edu Tue Sep 1 09:39:02 2009
9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n81Ed1wU025575
9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 09:39:01 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAB/PnErRauUp/2dsb2JhbADNeQEJhneISoJMgU8F
9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
9, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 01 Sep 2009 09:39:00 -0500
9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 27 -- Tue, 1 Sep 2009 09:38:59 -0500
9, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5
9, 27 -- Content-class: urn:content-classes:message
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Type: text/plain;
9, 27 -- charset="us-ascii"
9, 27 -- Subject: LM: Stain for myelin in plastic sections
9, 27 -- Date: Tue, 1 Sep 2009 09:38:59 -0500
9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4107B9F3E6-at-UM-XMAIL08.um.umsystem.edu}
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- Thread-Topic: LM: Stain for myelin in plastic sections
9, 27 -- Thread-Index: AcorEfEHybeJ5ZUSQH2miEkq6XGo+g==
9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 27 -- To: {microscopy-at-microscopy.com}
9, 27 -- X-OriginalArrivalTime: 01 Sep 2009 14:39:00.0110 (UTC) FILETIME=[F196A2E0:01CA2B11]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n81Ed1wU025575
==============================End of - Headers==============================




From: dcromey-at-email.arizona.edu
Date: Tue, 1 Sep 2009 10:11:30 -0500
Subject: [Microscopy] LM: Stain for myelin in plastic sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

It's been 25 years since I worked for a neuropathologist, but here's what I
remember (and could dig up):

We did a lot of morphometry (in Black & White film), so the
paraphenylenediamine stain (lipids stain dark brown over a light brown
background) worked well. We used a 1% aqueous solution (in distilled H2O,
vigorous stirring to dissolve everything). As I recall (it's not written
down) staining occurred on the slide, on a 80 degrees C hot plate for
15-30sec and then rinse with distilled H2O. This was a very dependable, if
not very exciting looking stain.

We also used 1% T Blue (in 1% Na borate), but not often.

The pathologist's favorite (because it looked like H&E) was the Paragon
stain. 0.73g toluidine blue, 0.135g basic fuchsin in 100ml of 30% Ethanol.
The staining protocol was a bit different for Epon-like plastics, as opposed
to Spurrs. For Epon plastics, we stained on the hot plate for about 15-30
secs, rinsed gently with distilled H2O and then we checked the stain on a
scope while it was still wet. Sometimes it needed longer, so it was back to
the hotplate. Spurrs was exposed to the stain for only 10-15 secs, with a
light sprinkle of Na borate powder on top of the stain and then rinsed with
distilled water. I seem to recall this was a temperamental stain and
difficult to get "just right".

Have fun.
Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA

office: AHSC 4212 email: Cromey-at-Arizona.edu
voice: 520-626-2824 fax: 520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Tuesday, September 01, 2009 7:40 AM
To: dcromey-at-email.arizona.edu

Dear Collective,

Does anyone have a favorite stain for myelin in plastic thick sections?


We have been given one protocol using P-phenylene diamine in absolute
ethanol, and we will give it a try, but if there are any other pet
techniques that you would be willing to share, we would love to try
them, too.

Thanks!

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original Headers==============================
9, 27 -- From TindallR-at-missouri.edu Tue Sep 1 09:39:02 2009
9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu
(mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n81Ed1wU025575
9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 09:39:01
-0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result:
ApoEAB/PnErRauUp/2dsb2JhbADNeQEJhneISoJMgU8F
9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
([209.106.229.41])
9, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 01 Sep 2009
09:39:00 -0500
9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by
um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 27 -- Tue, 1 Sep 2009 09:38:59 -0500
9, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5
9, 27 -- Content-class: urn:content-classes:message
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Type: text/plain;
9, 27 -- charset="us-ascii"
9, 27 -- Subject: LM: Stain for myelin in plastic sections
9, 27 -- Date: Tue, 1 Sep 2009 09:38:59 -0500
9, 27 -- Message-ID:
{91108EF9255B394CBF8B7E3789814A4107B9F3E6-at-UM-XMAIL08.um.umsystem.edu}
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- Thread-Topic: LM: Stain for myelin in plastic sections
9, 27 -- Thread-Index: AcorEfEHybeJ5ZUSQH2miEkq6XGo+g==
9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 27 -- To: {microscopy-at-microscopy.com}
9, 27 -- X-OriginalArrivalTime: 01 Sep 2009 14:39:00.0110 (UTC)
FILETIME=[F196A2E0:01CA2B11]
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n81Ed1wU025575
==============================End of - Headers==============================



==============================Original Headers==============================
25, 28 -- From dcromey-at-email.arizona.edu Tue Sep 1 10:11:29 2009
25, 28 -- Received: from mailgator.email.arizona.edu (pacer.email.arizona.edu [128.196.133.172])
25, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n81FBSOs008595
25, 28 -- for {Microscopy-at-Microscopy.Com} ; Tue, 1 Sep 2009 10:11:29 -0500
25, 28 -- Received: from mailgators_amavis (amavis1.email.arizona.edu [10.0.0.204])
25, 28 -- by mailgator.email.arizona.edu (Postfix) with ESMTP id E6D60342B554;
25, 28 -- Tue, 1 Sep 2009 08:11:26 -0700 (MST)
25, 28 -- Received: from RASTER (hepatic1.cba.arizona.edu [128.196.157.6])
25, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id C242D128B4B7;
25, 28 -- Tue, 1 Sep 2009 08:11:24 -0700 (MST)
25, 28 -- Reply-To: {cromey-at-arizona.edu}
25, 28 -- From: "Doug Cromey" {dcromey-at-email.arizona.edu}
25, 28 -- To: {TindallR-at-missouri.edu}
25, 28 -- Cc: "Microscopy listserv" {Microscopy-at-Microscopy.Com}
25, 28 -- References: {200909011440.n81Ee8Hc026424-at-ns.microscopy.com}
25, 28 -- In-Reply-To: {200909011440.n81Ee8Hc026424-at-ns.microscopy.com}
25, 28 -- Subject: RE: [Microscopy] LM: Stain for myelin in plastic sections
25, 28 -- Date: Tue, 1 Sep 2009 08:11:23 -0700
25, 28 -- Organization: University of Arizona
25, 28 -- Message-ID: {002001ca2b16$7960c3a0$6c224ae0$-at-arizona.edu}
25, 28 -- MIME-Version: 1.0
25, 28 -- Content-Type: text/plain;
25, 28 -- charset="us-ascii"
25, 28 -- Content-Transfer-Encoding: 7bit
25, 28 -- X-Mailer: Microsoft Office Outlook 12.0
25, 28 -- Thread-Index: AcorEh0IvJ7W7bY5Qn2+iAVukRwNrgAAgP8A
25, 28 -- Content-Language: en-us
25, 28 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: Linda.Nikolova-at-hsc.utah.edu
Date: Tue, 1 Sep 2009 13:06:18 -0500
Subject: [Microscopy] viaWWW: IMOD software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Linda.Nikolova-at-hsc.utah.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Linda.Nikolova-at-hsc.utah.edu
Name: Linda

Organization: University of Utah, EIHG

Title-Subject: [Filtered] IMOD software

Question: Dear Shannon,

I am not aware of any workshops on the IMOD software but you can
always contact the people created the IMOD, at the U of Colorado
Boulder. They will help you with any question arising on the usage of
this software. IMOD is not easy to use, but it is not impossible to
learn. They will provide you with a very good manual. I attended the
Cryo Course at HITACHI, Pleasanton, CA and we had a excellent teacher
on IMOD, her name is Eileen O'Toeele. You may try to e-mail her and
ask her those questions.

L.

Login Host: 155.101.149.88
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Sep 1 13:06:18 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n81I6HV7031890
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 13:06:17 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240803c6c3130aa842-at-[206.69.208.22]}
8, 11 -- Date: Tue, 1 Sep 2009 13:06:15 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: Linda.Nikolova-at-hsc.utah.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: IMOD software
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: hubner-at-m6.mech.pk.edu.pl
Date: Wed, 2 Sep 2009 03:24:58 -0500
Subject: [Microscopy] ICCS 2010 - Call for papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ************** ICCS tenth anniversary ***************
} * ICCS 2010
} * Tenth International Conference on Computational Science
} * Amsterdam, The Netherlands
} * May 31 - June 2, 2010
} *
} * http://www.iccs-meeting.org/iccs2010
} *
} ******************************************************
} In 2010 ICCS celebrates its 10th anniversary in Amsterdam.
} For this great event we will have several world leading keynote speakers
} to give their vision on Computational Science now and in the future.
}
} You are invited to submit a paper with unpublished original work
} for ICCS 2010, Amsterdam on May 31 - June 2, 2010.
} You can submit to the main conference or one of the workshops.
} All accepted oral papers will appear in the proceedings.
}
} Please, see http://www.iccs-meeting.org/iccs2010/ for more information,
} including our calls for workshops.
}
} The theme for ICCS 2010 in Amsterdam is "Advancing Computational
} Thinking", to mark several decades of progress in computational science
} theory and practice, leading to greatly improved applications in
} science. This conference will be a unique event focusing on recent
} developments in methods and modelling of complex systems for diverse
} areas of science, scalable scientific algorithms, advanced software
} tools, computational grids, advanced numerical methods, and novel
} application areas where the above novel models, algorithms and tools can
} be efficiently applied such as physical systems, computational and
} systems biology, environmental systems, finance, and others. We look
} forward to welcoming you to this exciting event!
}
} The ICCS 2010 Proceedings will be published by Elsevier in the
} Procedia Computer Science open-access series and on CD. In addition
} selected papers will be published in various peer reviewed Journals.
}
} Important dates:
} Full papers submission January 1, 2010
} Notification of acceptance of papers February 15, 2010
} Camera ready papers March 1, 2010
} Early registration opens February 15, 2010
} Early registration closes March 31, 2010
}
} Scientific Chair Peter Sloot
} Workshop Chair Dick van Albada
} Scientific Co-chair Jack Dongarra
}
} --
} We apologize for any cross postings. To remove yourself from our mailing
} list send a message to {listserv-at-nic.surfnet.nl} with as body
} UNSUBSCRIBE ICCS-CONF-SECR
}



==============================Original Headers==============================
4, 31 -- From hubner-at-m6.mech.pk.edu.pl Wed Sep 2 03:24:57 2009
4, 31 -- Received: from m6.mech.pk.edu.pl (m6.mech.pk.edu.pl [149.156.153.154])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n828OvEh012872
4, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Sep 2009 03:24:57 -0500
4, 31 -- Received: from localhost (unknown [127.0.0.1])
4, 31 -- by m6.mech.pk.edu.pl (Postfix) with ESMTP id 57409613;
4, 31 -- Wed, 2 Sep 2009 10:54:12 +0200 (MEST)
4, 31 -- Received: from m6.mech.pk.edu.pl ([127.0.0.1])
4, 31 -- by localhost (m6 [127.0.0.1]) (amavisd-new, port 10024) with ESMTP
4, 31 -- id 06173-07; Wed, 2 Sep 2009 10:53:40 +0200 (MEST)
4, 31 -- Received: from Krzysiek (unknown [149.156.153.184])
4, 31 -- by m6.mech.pk.edu.pl (Postfix) with SMTP id D57FF5D6;
4, 31 -- Wed, 2 Sep 2009 10:53:37 +0200 (MEST)
4, 31 -- Message-ID: {778ABAB572294392B869F1190010BC7E-at-Krzysiek}
4, 31 -- From: =?ISO-8859-1?Q?Krzysztof_H=FCbner?= {hubner-at-m6.mech.pk.edu.pl}
4, 31 -- To: "Materials" {MATERIALS-at-liverpool.ac.uk} ,
4, 31 -- "Microscopy list" {microscopy-at-microscopy.com} , {odLEW-PL-at-man.lodz.pl}
4, 31 -- Subject: ICCS 2010 - Call for papers
4, 31 -- Date: Wed, 2 Sep 2009 10:24:19 +0200
4, 31 -- Organization: =?ISO-8859-1?Q?Politechnika_Krakowska_Wydzia=B3_Mechaniczny?=
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- format=flowed;
4, 31 -- charset="ISO-8859-1";
4, 31 -- reply-type=response
4, 31 -- Content-Transfer-Encoding: 7bit
4, 31 -- X-Priority: 3
4, 31 -- X-MSMail-Priority: Normal
4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843
4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
4, 31 -- X-Virus-Scanned: by amavisd-new at m6.mech.pk.edu.pl
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 2 Sep 2009 06:50:05 -0500
Subject: [Microscopy] jeol sem water cooling tubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yorgos,
Yes, counter-current systems are very efficient! I would separate the
supply and return loops. I've seen many JEOL instruments with these large
coils of water lines. I would also be inclined to eliminate most of the
extra tubing, also, but separating the 2 loops is a must.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr]
Sent: Tuesday, September 01, 2009 12:16 AM
To: kenconverse-at-qualityimages.biz

Dear Friends
I noticed that the water tubes coming in and out of my JSM 5600 LV
microscope were twisted together in several circles. Close contact of the
two tubes results to a counter-current mechanism of heat exchange IinI {-
IoutI that reduces drastically the efficiency of cooling and increases water

flow requirements for cooling. On the other hand it prevents very cold water

from reaching the pump. This efficient mechanism is active in the
extremities of all hot-blooded animals by means of close contact between
veins and arteries.
Does anybody know:
1. Are the tubes put together on some purpose by jeol or it just happened to

my microscope (bought second hand but probably decommissioned by jeol)?
2. Can I separate the tubes and save water in my place where tap water never

has very low temperatures?
Regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477


==============================Original Headers==============================
4, 22 -- From eikonika-at-otenet.gr Mon Aug 31 23:12:47 2009
4, 22 -- Received: from kane.otenet.gr (kane.otenet.gr [83.235.67.31])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n814Ck8C015194
4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 23:12:47
-0500
4, 22 -- Received: from axeron (athedsl-322635.home.otenet.gr
[85.72.115.233])
4, 22 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id
n814Cjoc002490
4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 07:12:45
+0300
4, 22 -- Message-ID: {001701ca2aba$80dcfb50$1601a8c0-at-axeron}
4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
4, 22 -- To: {microscopy-at-microscopy.com}
4, 22 -- Subject: jeol sem water cooling tubes
4, 22 -- Date: Tue, 1 Sep 2009 07:12:32 +0300
4, 22 -- MIME-Version: 1.0
4, 22 -- Content-Type: text/plain;
4, 22 -- format=flowed;
4, 22 -- charset="iso-8859-7";
4, 22 -- reply-type=original
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- X-Priority: 3
4, 22 -- X-MSMail-Priority: Normal
4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




==============================Original Headers==============================
20, 25 -- From kenconverse-at-qualityimages.biz Wed Sep 2 06:50:04 2009
20, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n82Bo2UA002501
20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Sep 2009 06:50:03 -0500
20, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
20, 25 -- with ESMTP
20, 25 -- id {20090902115001323.WVLV8054-at-cdptpa-omta01.mail.rr.com} ;
20, 25 -- Wed, 2 Sep 2009 11:50:01 +0000
20, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
20, 25 -- To: {eikonika-at-otenet.gr} , "MSA Listserver" {microscopy-at-microscopy.com}
20, 25 -- Subject: RE: [Microscopy] jeol sem water cooling tubes
20, 25 -- Date: Wed, 2 Sep 2009 07:49:40 -0400
20, 25 -- Message-ID: {3664C550DB0F4AA7974CB2D7BF50D2FA-at-Ken}
20, 25 -- MIME-Version: 1.0
20, 25 -- Content-Type: text/plain;
20, 25 -- charset="us-ascii"
20, 25 -- X-Priority: 3 (Normal)
20, 25 -- X-MSMail-Priority: Normal
20, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
20, 25 -- Importance: Normal
20, 25 -- Thread-Index: Acoqutie4r1evVeUS/a+gda7qZIf6ABCA0VQ
20, 25 -- In-Reply-To: {200909010415.n814FXI0019054-at-ns.microscopy.com}
20, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
20, 25 -- Content-Transfer-Encoding: 8bit
20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n82Bo2UA002501
==============================End of - Headers==============================




From: michelle.gignac-at-duke.edu
Date: Wed, 2 Sep 2009 16:30:37 -0500
Subject: [Microscopy] viaWWW: Silver nano-particle enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both michelle.gignac-at-duke.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: michelle.gignac-at-duke.edu
Name: Michelle

Organization: Duke University

Title-Subject: [Filtered] Silver nano-particle enhancement

Question: Hello. I have an investigator that is working with silver
nano-particles in tissue that are about 10nm. He would like to view
these with both the TEM and light microscope. Does anyone have a
method to enhance silver nano-particles?

Thanks,
Michelle

Michelle Gignac
EM Engineer
Shared Materials Instrumentation Facility (SMIF)
Duke University
Box 90271
Durham, NC 27708-0271
Phone: (919) 660-5477
Fax: (919) 660-5491
http://smif.lab.duke.edu


Login Host: 152.3.194.231
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Sep 2 16:30:37 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n82LUa00009912
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 2 Sep 2009 16:30:37 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c6c4946dfd36-at-[206.69.208.22]}
9, 11 -- Date: Wed, 2 Sep 2009 16:30:36 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: michelle.gignac-at-duke.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Silver nano-particle enhancement
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Wed, 2 Sep 2009 16:52:29 -0500
Subject: [Microscopy] viaWWW: Silver nano-particle enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe silver particles can be enhanced by the same techniques used
for colloidal gold. I like the GoldEnhance kit from Nanoprobes for both
LM an EM work. Check out www.nanoprobes.com.

Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/




-----Original Message-----
X-from: michelle.gignac-at-duke.edu [mailto:michelle.gignac-at-duke.edu]
Sent: Wednesday, September 02, 2009 4:32 PM
To: Phillips, Thomas E.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both michelle.gignac-at-duke.edu as well as the
MIcroscopy Listserver
------------------------------------------------------------------------
---

Email: michelle.gignac-at-duke.edu
Name: Michelle

Organization: Duke University

Title-Subject: [Filtered] Silver nano-particle enhancement

Question: Hello. I have an investigator that is working with silver
nano-particles in tissue that are about 10nm. He would like to view
these with both the TEM and light microscope. Does anyone have a
method to enhance silver nano-particles?

Thanks,
Michelle

Michelle Gignac
EM Engineer
Shared Materials Instrumentation Facility (SMIF)
Duke University
Box 90271
Durham, NC 27708-0271
Phone: (919) 660-5477
Fax: (919) 660-5491
http://smif.lab.duke.edu


Login Host: 152.3.194.231
------------------------------------------------------------------------
---

==============================Original
Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Sep 2 16:30:37 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n82LUa00009912
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 2 Sep 2009
16:30:37 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c6c4946dfd36-at-[206.69.208.22]}
9, 11 -- Date: Wed, 2 Sep 2009 16:30:36 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: michelle.gignac-at-duke.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Silver nano-particle enhancement
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
24, 29 -- From PhillipsT-at-missouri.edu Wed Sep 2 16:52:29 2009
24, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n82LqSEU024678
24, 29 -- for {microscopy-at-microscopy.com} ; Wed, 2 Sep 2009 16:52:28 -0500
24, 29 -- X-IronPort-Anti-Spam-Filtered: true
24, 29 -- X-IronPort-Anti-Spam-Result: ApoEAFeGnkrRauUo/2dsb2JhbADMPgEBAQeHXYhKAoJKAQGBTQWBV4kG
24, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
24, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 02 Sep 2009 16:52:28 -0500
24, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
24, 29 -- Wed, 2 Sep 2009 16:52:28 -0500
24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
24, 29 -- Content-class: urn:content-classes:message
24, 29 -- MIME-Version: 1.0
24, 29 -- Content-Type: text/plain;
24, 29 -- charset="us-ascii"
24, 29 -- Subject: RE: [Microscopy] viaWWW: Silver nano-particle enhancement
24, 29 -- Date: Wed, 2 Sep 2009 16:52:25 -0500
24, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD074983CF-at-UM-XMAIL06.um.umsystem.edu}
24, 29 -- In-Reply-To: {200909022131.n82LVeW4010970-at-ns.microscopy.com}
24, 29 -- X-MS-Has-Attach:
24, 29 -- X-MS-TNEF-Correlator:
24, 29 -- Thread-Topic: [Microscopy] viaWWW: Silver nano-particle enhancement
24, 29 -- thread-index: AcosFMTj+6T78SvWRoiewRO90v7hgQAAokqg
24, 29 -- References: {200909022131.n82LVeW4010970-at-ns.microscopy.com}
24, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
24, 29 -- To: {michelle.gignac-at-duke.edu} , {microscopy-at-microscopy.com}
24, 29 -- X-OriginalArrivalTime: 02 Sep 2009 21:52:28.0195 (UTC) FILETIME=[AA073730:01CA2C17]
24, 29 -- Content-Transfer-Encoding: 8bit
24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n82LqSEU024678
==============================End of - Headers==============================




From: dcristofori-at-unive.it
Date: Thu, 3 Sep 2009 13:16:48 -0500
Subject: [Microscopy] Re: jeol sem water cooling tubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Yorgos,
I'm not as experienced as Ken, but I can tell you that our JSM-56000LV
has the the water tubes well separated, and that Jeol engineers have
never mentioned to couple them in the way you described.
Regards,

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive.it

Universita' Ca' Foscari Venezia
Dipartimento di Chimica Fisica
Lab. di Scienza e Tecnologia dei Materiali
Via Torino, 155b
I-30172 Mestre (VE)
Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

eikonika-at-otenet.gr ha scritto:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Friends
} I noticed that the water tubes coming in and out of my JSM 5600 LV
} microscope were twisted together in several circles. Close contact of the
} two tubes results to a counter-current mechanism of heat exchange IinI {-
} IoutI that reduces drastically the efficiency of cooling and increases water
} flow requirements for cooling. On the other hand it prevents very cold water
} from reaching the pump. This efficient mechanism is active in the
} extremities of all hot-blooded animals by means of close contact between
} veins and arteries.
} Does anybody know:
} 1. Are the tubes put together on some purpose by jeol or it just happened to
} my microscope (bought second hand but probably decommissioned by jeol)?
} 2. Can I separate the tubes and save water in my place where tap water never
} has very low temperatures?
} Regards
} yorgos
}
} Dr Yorgos Nikas
} Athens Innovative Microscopy
} Skra 36 Voula 16673 GREECE
}
} eikonika-at-otenet.gr
} yorgosnikas-at-hotmail.com
} Tel/fax +30 210 8957677
} Mobile +30 6945 107477
}
}
} ==============================Original Headers==============================
} 4, 22 -- From eikonika-at-otenet.gr Mon Aug 31 23:12:47 2009
} 4, 22 -- Received: from kane.otenet.gr (kane.otenet.gr [83.235.67.31])
} 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n814Ck8C015194
} 4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2009 23:12:47 -0500
} 4, 22 -- Received: from axeron (athedsl-322635.home.otenet.gr [85.72.115.233])
} 4, 22 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id n814Cjoc002490
} 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Sep 2009 07:12:45 +0300
} 4, 22 -- Message-ID: {001701ca2aba$80dcfb50$1601a8c0-at-axeron}
} 4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
} 4, 22 -- To: {microscopy-at-microscopy.com}
} 4, 22 -- Subject: jeol sem water cooling tubes
} 4, 22 -- Date: Tue, 1 Sep 2009 07:12:32 +0300
} 4, 22 -- MIME-Version: 1.0
} 4, 22 -- Content-Type: text/plain;
} 4, 22 -- format=flowed;
} 4, 22 -- charset="iso-8859-7";
} 4, 22 -- reply-type=original
} 4, 22 -- Content-Transfer-Encoding: 7bit
} 4, 22 -- X-Priority: 3
} 4, 22 -- X-MSMail-Priority: Normal
} 4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
} 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
} ==============================End of - Headers==============================


==============================Original Headers==============================
8, 28 -- From dcristofori-at-unive.it Thu Sep 3 13:16:48 2009
8, 28 -- Received: from ariel.unive.it (ariel.unive.it [157.138.1.34])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n83IGlaW001784
8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Sep 2009 13:16:48 -0500
8, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
8, 28 -- by ariel.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n83IGlTp008913
8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Sep 2009 20:16:47 +0200
8, 28 -- Received: from ariel.unive.it ([127.0.0.1])
8, 28 -- by localhost (ariel.unive.it [127.0.0.1]) (amavisd-new, port 10024)
8, 28 -- with LMTP id jxsvV5ouAeFb for {microscopy-at-microscopy.com} ;
8, 28 -- Thu, 3 Sep 2009 20:16:47 +0200 (CEST)
8, 28 -- Received: from helios.unive.it (helios.unive.it [157.138.8.4])
8, 28 -- by ariel.unive.it (8.12.11.20060308/8.12.11) with ESMTP id n83IGdvs008909
8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Sep 2009 20:16:43 +0200
8, 28 -- Received: from [127.0.0.1] (ferroni.dcf.unive.it [157.138.23.68])
8, 28 -- by helios.unive.it (8.13.8/8.13.8) with ESMTP id n83IHEtk024305
8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Sep 2009 20:17:25 +0200
8, 28 -- Message-ID: {4AA00ACC.7060200-at-unive.it}
8, 28 -- Date: Thu, 03 Sep 2009 20:28:28 +0200
8, 28 -- From: Davide Cristofori {dcristofori-at-unive.it}
8, 28 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
8, 28 -- MIME-Version: 1.0
8, 28 -- To: microscopy-at-microscopy.com
8, 28 -- Subject: Re: [Microscopy] jeol sem water cooling tubes
8, 28 -- References: {200909010418.n814INF1024742-at-ns.microscopy.com}
8, 28 -- In-Reply-To: {200909010418.n814INF1024742-at-ns.microscopy.com}
8, 28 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
8, 28 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: bernard-at-berkeleyrc.com
Date: Thu, 3 Sep 2009 13:48:15 -0500
Subject: [Microscopy] jeol sem water cooling tubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is no need to put the tubes in thermal contact, and it is
counter-productive. I would assume you found it this way because they
packed it that way. You should get a chiller to avoid wasting water.
In living tissue it serves the purpose of thermostasis.

} } Dear Friends
} } I noticed that the water tubes coming in and out of my JSM 5600 LV
} } microscope were twisted together in several circles. Close contact of the
} } two tubes results to a counter-current mechanism of heat exchange IinI {-
} } IoutI that reduces drastically the efficiency of cooling and increases water
} } flow requirements for cooling. On the other hand it prevents very cold water
} } from reaching the pump. This efficient mechanism is active in the
} } extremities of all hot-blooded animals by means of close contact between
} } veins and arteries.
} } Does anybody know:
} } 1. Are the tubes put together on some purpose by jeol or it just happened to
} } my microscope (bought second hand but probably decommissioned by jeol)?
} } 2. Can I separate the tubes and save water in my place where tap water never
} } has very low temperatures?
} } Regards
} } yorgos



--
Bernard R. Cuzzillo, Ph.D., P.E.
President, Mechanical Engineer, and Fire Scientist
Berkeley Research Company (BRC)
600 Addison Street
Berkeley, CA  94710-1920
USA

www.berkeleyrc.com

bernard-at-berkeleyrc.com

Cell phone: 510.821.2499
Office phone: 510.868.4333
Fax: 510.868.4351


==============================Original Headers==============================
9, 38 -- From biggerdadda-at-gmail.com Thu Sep 3 13:48:15 2009
9, 38 -- Received: from mail-yw0-f175.google.com (mail-yw0-f175.google.com [209.85.211.175])
9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n83ImF4C017109
9, 38 -- for {microscopy-at-microscopy.com} ; Thu, 3 Sep 2009 13:48:15 -0500
9, 38 -- Received: by ywh5 with SMTP id 5so97418ywh.4
9, 38 -- for {microscopy-at-microscopy.com} ; Thu, 03 Sep 2009 11:48:15 -0700 (PDT)
9, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
9, 38 -- d=gmail.com; s=gamma;
9, 38 -- h=domainkey-signature:mime-version:sender:received:in-reply-to
9, 38 -- :references:date:x-google-sender-auth:message-id:subject:from:to
9, 38 -- :content-type:content-transfer-encoding;
9, 38 -- bh=RSujEVe4y0f5VvmI4a2MLrVPxxUy4700FVkXbYpoCno=;
9, 38 -- b=XFXJbRdKHa5+83F1xI3ktWzXL3kIh2PtSPy8PeUT5rdwxHq56bnrAsuVs/uv+0enwn
9, 38 -- D+XWRAQH5fGhODRHfQdy+EeDVI8iQkIlJbwqHFmKiPUl7e62dHC6GocER02Ck8xF7jWn
9, 38 -- XC4s2rv5DvimI8tHtl8icQRkgT9+z8gRu1/s0=
9, 38 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
9, 38 -- d=gmail.com; s=gamma;
9, 38 -- h=mime-version:sender:in-reply-to:references:date
9, 38 -- :x-google-sender-auth:message-id:subject:from:to:content-type
9, 38 -- :content-transfer-encoding;
9, 38 -- b=gg8SPGdff5+PE7PJHkSvmezRAwYcymM5EfxSxmiEBMd7/yOwtwDHl5RclID75DOS1q
9, 38 -- BloJro2KJblhhHgBzhSy/mkQmeDjM+Hvl2xdhg05aQmiZa1pwbp5fm+hgvEa2Q8ax3ML
9, 38 -- p6tvxGrWMiUYQ9J9l/ijQQo+4iPl42O0sA/nA=
9, 38 -- MIME-Version: 1.0
9, 38 -- Sender: biggerdadda-at-gmail.com
9, 38 -- Received: by 10.101.33.5 with SMTP id l5mr11268998anj.44.1252003694866; Thu,
9, 38 -- 03 Sep 2009 11:48:14 -0700 (PDT)
9, 38 -- In-Reply-To: {200909031823.n83IN4gQ010404-at-ns.microscopy.com}
9, 38 -- References: {200909031823.n83IN4gQ010404-at-ns.microscopy.com}
9, 38 -- Date: Thu, 3 Sep 2009 11:48:14 -0700
9, 38 -- X-Google-Sender-Auth: 9f1c27e9486ca1fa
9, 38 -- Message-ID: {ef580e610909031148k25accb86m9124b2c758b15e29-at-mail.gmail.com}
9, 38 -- Subject: Re: [Microscopy] Re: jeol sem water cooling tubes
9, 38 -- From: "Bernard R. Cuzzillo, Ph.D., P.E." {bernard-at-berkeleyrc.com}
9, 38 -- To: microscopy-at-microscopy.com
9, 38 -- Content-Type: text/plain; charset=ISO-8859-1
9, 38 -- Content-Transfer-Encoding: 8bit
9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n83ImF4C017109
==============================End of - Headers==============================




From: z.zhou-at-sheffield.ac.uk
Date: Fri, 4 Sep 2009 09:07:31 -0500
Subject: [Microscopy] viaWWW: TEM powder sample prep by ion-milling?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both z.zhou-at-sheffield.ac.uk as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: z.zhou-at-sheffield.ac.uk
Name: Zhaoxia

Organization: The University of Sheffield, UK

Title-Subject: [Filtered] TEM powder sample prep by ion-milling?

Question: Hello. I have got some LiCoMnO4 powder for TEM/EELS study.
The particles sizes are 0.5-5 microns crushed by pestel and mortar.
The TEM samples prepared by using ultrasonic dispersion turns out to
be too thick for EELS studies.

Some people suggested mix the powder in epoxy and use ion-mill to get
thin area.

Could anyone please advise me with more details about this method? Or
other methods to thin the fine particles?

Thanks,

Zhaoxia Zhou
Dept Engineering Materials,
The University of Sheffield,
Sheffield, UK




Login Host: 143.167.181.8
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Fri Sep 4 09:07:30 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84E7U6R030877
13, 11 -- for {microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 09:07:30 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240802c6c6cf7fd821-at-[206.69.208.22]}
13, 11 -- Date: Fri, 4 Sep 2009 09:07:29 -0500
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: z.zhou-at-sheffield.ac.uk (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: TEM powder sample prep by ion-milling?
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cw23-at-ohm.york.ac.uk
Date: Fri, 4 Sep 2009 09:30:38 -0500
Subject: [Microscopy] Circuit diagrams for Hitachi S-2400 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Does anyone have the circuit diagrams for the Hitachi S-2400 SEM
available (e.g. as a pdf or jpeg) which they could email to me. We seem
to have mislaid our copies.

Many thanks
Chris Walker


==============================Original Headers==============================
3, 25 -- From cw23-at-ohm.york.ac.uk Fri Sep 4 09:30:37 2009
3, 25 -- Received: from mail.elec.york.ac.uk (mail.elec.york.ac.uk [144.32.138.107])
3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84EUbE4017038
3, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 4 Sep 2009 09:30:37 -0500
3, 25 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
3, 25 -- by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
3, 25 -- id 1MjZo9-0003E7-Fw
3, 25 -- for Microscopy-at-Microscopy.Com; Fri, 04 Sep 2009 15:30:33 +0100
3, 25 -- Message-ID: {4AA1247B.4090005-at-ohm.york.ac.uk}
3, 25 -- Date: Fri, 04 Sep 2009 15:30:19 +0100
3, 25 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
3, 25 -- Organization: Department of Electronics, The University of York
3, 25 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 25 -- MIME-Version: 1.0
3, 25 -- To: Microscopy-at-Microscopy.Com
3, 25 -- Subject: Circuit diagrams for Hitachi S-2400 SEM
3, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 25 -- Content-Transfer-Encoding: 7bit
3, 25 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
3, 25 -- X-YorkElec-MailScanner-ID: 1MjZo9-0003E7-Fw
3, 25 -- X-YorkElec-MailScanner: Found to be clean
3, 25 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
3, 25 -- X-YorkElec-MailScanner-Watermark: 1252679436.25436-at-J85sA1lTLQkf1XGIg8adrA
3, 25 -- X-Spam-Status: No
3, 25 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
==============================End of - Headers==============================




From: hstahlberg-at-me.com
Date: Fri, 4 Sep 2009 11:37:36 -0500
Subject: [Microscopy] Cryo-EM Faculty Position: Molecular and Cellular Biology, UC Davis,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Cryo-Electron Microscopy
Faculty Position
University of California, Davis
Department of Molecular and Cellular Biology

The Department of Molecular and Cellular Biology at University of
California, Davis (http://www.mcb.ucdavis.edu/) invites applications
for a faculty position in Cryo-Electron Microscopy at the assistant
(tenure-track), associate or full professor level. We seek candidates
with exceptional research achievements that have the ability to
develop a cutting-edge and well-funded independent research program
and that show a commitment to excellence in research and education.
Candidates should have a Ph.D. and postdoctoral experience.

The applicant's research program should involve the application of
cryo-electron microscopy to address novel structuuestions relating to
biochemistry, membrane biology, cell biology, molecular biology,
neurobiology, microbiology, or other fields. A method development
component in a biological research program is also welcomed. This
position will be housed the Briggs building, which has a state-of-the-
art electron microscopy facility. Current equipment includes several
TEMs, among them a JEM-3000SFF (300kV FEG, helium cooled top-entry
stage), a JEM-2100F (200kV FEG (S)TEM with 4k CCD), an aberration-
corrected JEM-2100F (200kV FEG, probe-corrected (S)TEM with GIF), and
a JEM1230. All are cryo-EM capable, the latter three offer electron
tomography data collection. For detailed information, please contact
Jon Scholey, MCB (JMScholey-at-ucdavis.edu) or Nigel Browning, CHMS (NBrowning-at-ucdavis.edu
).

Candidates should submit the following items online at http://www.mcb.ucdavis.edu
. A curriculum vitae, a 1-2 page summary of research
accomplishments, a 1-2 page description of future research plans,
copies of one to three major publications, and a statement of teaching
experience and/or interest. Candidates should also arrange for three
letters of recommendations to be submitted online at the same website
address.

This search will remain open until filled. However, applications
should be received by October 15, 2009 for full consideration.

The University of California, Davis, is an Equal Opportunity/
Affirmative Action Employer.



==============================Original Headers==============================
10, 22 -- From hstahlberg-at-me.com Fri Sep 4 11:37:36 2009
10, 22 -- Received: from asmtpout012.mac.com (asmtpout012.mac.com [17.148.16.87])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84GbZkd008349
10, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 11:37:36 -0500
10, 22 -- MIME-version: 1.0
10, 22 -- Content-transfer-encoding: 7BIT
10, 22 -- Content-type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 22 -- Received: from bs-mp03.ethz.ch (bs-mp03.ethz.ch [129.132.128.96])
10, 22 -- by asmtp012.mac.com
10, 22 -- (Sun Java(tm) System Messaging Server 6.3-8.01 (built Dec 16 2008; 32bit))
10, 22 -- with ESMTPSA id {0KPG004RHGUERX90-at-asmtp012.mac.com} for
10, 22 -- Microscopy-at-microscopy.com; Fri, 04 Sep 2009 09:37:31 -0700 (PDT)
10, 22 -- Message-id: {6F7667DD-32BC-4B12-BA8F-33D57A245823-at-me.com}
10, 22 -- From: Henning Stahlberg {hstahlberg-at-me.com}
10, 22 -- To: Microscopy-at-microscopy.com
10, 22 -- Subject: Cryo-EM Faculty Position: Molecular and Cellular Biology, UC Davis,
10, 22 -- California
10, 22 -- Date: Fri, 04 Sep 2009 18:28:59 +0200
10, 22 -- Cc: Jodi Nunnari {JMNunnari-at-ucdavis.edu} ,
10, 22 -- Nigel Browning {nbrowning-at-ucdavis.edu} ,
10, 22 -- Jonathan Scholey {jmscholey-at-ucdavis.edu}
10, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: Robert.Zonis-at-Sanford.com
Date: Fri, 4 Sep 2009 14:22:09 -0500
Subject: [Microscopy] LM: Surplus Equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a use for an old Canlab stereo microscope? I also have a very basic no-name monocular microscope - both free to a good home.

Robert Zonis
Technical Service, LMTC
Sanford L.P. - A Newell Rubbermaid Company

Shelbyville, TN 37160
Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv and is the unofficial opinion of the writer only. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.


This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message. Please note that although we will take all commercially reasonable efforts to prevent viruses from being transmitted from our systems, it is the responsibility of the recipient to check for and prevent adverse action by viruses on its own systems.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________


==============================Original Headers==============================
8, 31 -- From Robert.Zonis-at-Sanford.com Fri Sep 4 14:22:09 2009
8, 31 -- Received: from mail142.messagelabs.com (mail142.messagelabs.com [216.82.249.99])
8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n84JM8tT028976
8, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 14:22:09 -0500
8, 31 -- X-VirusChecked: Checked
8, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com
8, 31 -- X-Msg-Ref: server-10.tower-142.messagelabs.com!1252092128!88214329!1
8, 31 -- X-StarScan-Version: 6.1.3; banners=sanford.com,-,-
8, 31 -- X-Originating-IP: [198.176.16.25]
8, 31 -- Received: (qmail 12239 invoked from network); 4 Sep 2009 19:22:08 -0000
8, 31 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25)
8, 31 -- by server-10.tower-142.messagelabs.com with SMTP; 4 Sep 2009 19:22:08 -0000
8, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830);
8, 31 -- Fri, 4 Sep 2009 14:22:07 -0500
8, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 31 -- Content-class: urn:content-classes:message
8, 31 -- MIME-Version: 1.0
8, 31 -- Content-Type: text/plain;
8, 31 -- charset="iso-8859-1"
8, 31 -- Subject: [Microscopy] LM: Surplus Equipment
8, 31 -- Date: Fri, 4 Sep 2009 14:19:16 -0500
8, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A940401A31C-at-naoaksasebe02.nr.ad.newellco.com}
8, 31 -- X-MS-Has-Attach:
8, 31 -- X-MS-TNEF-Correlator:
8, 31 -- Thread-Topic: [Microscopy] LM: Surplus Equipment
8, 31 -- Thread-Index: Acotk4R8ie8DysPJQhe6BCt2g+XE2QAAPloA
8, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com}
8, 31 -- To: {Microscopy-at-microscopy.com}
8, 31 -- X-OriginalArrivalTime: 04 Sep 2009 19:22:07.0661 (UTC) FILETIME=[FE3275D0:01CA2D94]
8, 31 -- Content-Transfer-Encoding: 8bit
8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n84JM8tT028976
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Fri, 4 Sep 2009 15:50:20 -0500
Subject: [Microscopy] Postdoctoral position - ORNL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

A new Energy Frontier Research Center for the study of Fluid Interface
Reactions, Structure and Transport (FIRST) at Oak Ridge National
Laboratory (http://www.ornl.gov/sci/first/index.shtml) is seeking a
post-doctoral associate to work on a research project in Scanning Probe
Microscopies of electrochemically-active materials. The research will
involve electrochemical and electromechanical studies of kinetics,
thermodynamics, and mechanisms of local electrochemical processes at
conducting and semiconducting surfaces in ambient environment, aqueous
and non-aqueous solution. The research will be conducted in
collaboration with other members of the FIRST Center in which
experimental studies will be integrated with theory, modeling, and
simulation. The expected product of this research is a spatially
resolved picture of electrochemical process in real space, which then
both serve as an input for macroscopic studies, and allows to link
defect and microstructure to functionality on mesoscopic and atomic
levels. The research will take advantage of unique set of SPM tools
developed at ORNL for the characterization of electrical, transport, and
dynamic properties of materials, including band excitation scanning
probe microscopy and multimodal switching spectroscopy measurements. The
ORNL group has a strong publication record including multiple
publications in high-profile journals, and offers an interdisciplinary
and exciting atmosphere of scientific discovery and exploration in the
area of nanoscale phenomena and functionality by SPM.

For more information about this position please contact Dr. Sergei V.
Kalinin (sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} ). Please reference
this position title in your correspondence.

Sergei

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
8, 24 -- From sergei2-at-ornl.gov Fri Sep 4 15:50:20 2009
8, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84KoKwV013570
8, 24 -- for {microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 15:50:20 -0500
8, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1])
8, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
8, 24 -- with ESMTP id {0KPG002HJSJV2S-at-emroute1.ornl.gov} for
8, 24 -- microscopy-at-microscopy.com; Fri, 04 Sep 2009 16:50:19 -0400 (EDT)
8, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov
8, 24 -- (PMDF V6.4 #31561) id {0KPG00501SJVRP-at-emroute1.ornl.gov} for
8, 24 -- microscopy-at-microscopy.com; Fri, 04 Sep 2009 16:50:19 -0400 (EDT)
8, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
8, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
8, 24 -- with ESMTP id {0KPG00K2FSJVO0-at-emroute1.ornl.gov} for
8, 24 -- microscopy-at-microscopy.com; Fri, 04 Sep 2009 16:50:19 -0400 (EDT)
8, 24 -- Date: Fri, 04 Sep 2009 16:50:19 -0400
8, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
8, 24 -- Subject: Postdoctoral position - ORNL
8, 24 -- To: microscopy-at-microscopy.com
8, 24 -- Message-id: {4AA17D8B.30309-at-ornl.gov}
8, 24 -- MIME-version: 1.0
8, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
8, 24 -- Content-transfer-encoding: 7bit
8, 24 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
==============================End of - Headers==============================




From: celikaktas-at-gmail.com
Date: Fri, 4 Sep 2009 16:26:20 -0500
Subject: [Microscopy] Re: z-height, focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

We got a 200 kV TEM. For a radiation sensitive sample I have to
operate this instrument at 80 or 100 kV. In this case I cannot bring
the sample to focus by changing z-height. Flipping sample does not
help either. It is away from focus about 100 micron. Then I use the
focus knob to bring the sample to focus. As a result, what kind of art
effects, distortions etc. should I expect?


Regards,
Ayten

--
===========================
Ayten Celik-Aktas, PhD
Ankara University
Electron Microscopy Laboratory
Ankara, Turkey
===========================


==============================Original Headers==============================
6, 35 -- From celikaktas-at-gmail.com Fri Sep 4 16:26:20 2009
6, 35 -- Received: from mail-fx0-f226.google.com (mail-fx0-f226.google.com [209.85.220.226])
6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84LQKtS028947
6, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 16:26:20 -0500
6, 35 -- Received: by fxm26 with SMTP id 26so984285fxm.18
6, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 04 Sep 2009 14:26:19 -0700 (PDT)
6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 35 -- d=gmail.com; s=gamma;
6, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
6, 35 -- :date:message-id:subject:from:to:content-type
6, 35 -- :content-transfer-encoding;
6, 35 -- bh=HvZXPDL6sPpEts0LIuPmpFwqYOOqYVZBctqBDmGJr2U=;
6, 35 -- b=huyhWFnHYK6vZg1ibhccn+Y7mlj8UL+YgNNgLN8YqDq+hOjq2XVZy0cz3WuHN9i2Hm
6, 35 -- uWI087pvVugIXRsIfsAcEf4yBHqwlZwKWlsgLb/nRHpBcLDc5PZfTp4TszN2/2FHCQv1
6, 35 -- cJltvkbpJnyBe+AwwgBMX4qrEzDpWPV8SZ/Ng=
6, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
6, 35 -- d=gmail.com; s=gamma;
6, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
6, 35 -- :content-type:content-transfer-encoding;
6, 35 -- b=ZtIqjs07tBKoG5qtXwcqdaNSr4jnO4qcdK8bAVFyDryP4s2nVzwvtr1NXTBvqZDeoV
6, 35 -- IjutzSmZHuWPITLJMk9PeHw9lNFoTmJuQNxCAY1aEP0UFddJyq8vW2v1Ye7u+NCxpVDY
6, 35 -- e8QGf/LHwSEEK64QE/W1EpEM/qmuMZ41ceSvY=
6, 35 -- MIME-Version: 1.0
6, 35 -- Received: by 10.204.160.80 with SMTP id m16mr9488180bkx.67.1252099579288; Fri,
6, 35 -- 04 Sep 2009 14:26:19 -0700 (PDT)
6, 35 -- In-Reply-To: {1075c5c10909040443u7a7983b8j50b21169faa0e237-at-mail.gmail.com}
6, 35 -- References: {1075c5c10909040443u7a7983b8j50b21169faa0e237-at-mail.gmail.com}
6, 35 -- Date: Sat, 5 Sep 2009 00:26:19 +0300
6, 35 -- Message-ID: {1075c5c10909041426l114216abob8ba4d9976667c8-at-mail.gmail.com}
6, 35 -- Subject: Re: z-height, focusing
6, 35 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com}
6, 35 -- To: microscopy {Microscopy-at-microscopy.com}
6, 35 -- Content-Type: text/plain; charset=UTF-8
6, 35 -- Content-Transfer-Encoding: 8bit
6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n84LQKtS028947
==============================End of - Headers==============================




From: tiekotte-at-up.edu
Date: Fri, 4 Sep 2009 18:06:47 -0500
Subject: [Microscopy] Polaroid/SterlingDx laser digital printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone want, either in total or for parts, a Polaroid/Sterling Dx laser
digital printer + film and laminate? The University of Portland is getting
rid of it due to an inability to connect it to the ethernet. It will be
heading to the trash on September 20th, 2009.

Thank you for your reply,

Regards,
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology / MSC 163
5000 N Willamette Blvd.
Portland, OR 97203 USA
Tel.: 503.943.8861
Email: tiekotte-at-up.edu






==============================Original Headers==============================
7, 22 -- From tiekotte-at-up.edu Fri Sep 4 18:06:47 2009
7, 22 -- Received: from egateone.up.edu (egateone.up.edu [64.251.254.100])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84N6kQY014850
7, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 18:06:47 -0500
7, 22 -- X-IronPort-AV: E=Sophos;i="4.44,335,1249282800";
7, 22 -- d="scan'208";a="27394071"
7, 22 -- Received: from unknown (HELO london.campus.up.edu) ([10.5.128.61])
7, 22 -- by egateone.up.edu with ESMTP; 04 Sep 2009 16:06:46 -0700
7, 22 -- Received: from 71.214.76.134 ([71.214.76.134]) by london.campus.up.edu ([10.5.128.61]) via Exchange Front-End Server webmail.up.edu ([10.10.1.43]) with Microsoft Exchange Server HTTP-DAV ;
7, 22 -- Fri, 4 Sep 2009 23:06:46 +0000
7, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122
7, 22 -- Date: Fri, 04 Sep 2009 16:07:14 -0700
7, 22 -- Subject: Polaroid/SterlingDx laser digital printer
7, 22 -- From: Ken Tiekotter {tiekotte-at-up.edu}
7, 22 -- To: {Microscopy-at-microscopy.com}
7, 22 -- Message-ID: {C6C6EBB2.DE4B%tiekotte-at-up.edu}
7, 22 -- Thread-Topic: Polaroid/SterlingDx laser digital printer
7, 22 -- Thread-Index: AcottHCarxg0+5mnEd6DIAADk7ZaIg==
7, 22 -- Mime-version: 1.0
7, 22 -- Content-type: text/plain;
7, 22 -- charset="US-ASCII"
7, 22 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: yngli-at-ucdavis.edu
Date: Sat, 5 Sep 2009 19:11:13 -0500
Subject: [Microscopy] viaWWW: Work parameters for JEOL JEM 2500 SE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Please reply to my email address.Thank you .

Kenneth L. Tiekotter,




------ Forwarded Message
X-from: Ken Tiekotter {tiekotte-at-up.edu}

Hi

Let us consider the problem a little deeper.

1. You are unable to adjust the specimen to the normal operating
position - the eucentric point I assume?

2. Changing the basic focal length of the objective lens changes the
magnification, the resolution and the contrast - if you require a higher
lens strength the focal length is shorter (higher magnification, higher
resolution but less contrast).

3. All that will change in your case is you seem to have a longer focal
length than normal, thus ,when you bring the sample in to focus using the
lens strength control, you are obtaining true focus ,but at a longer focal
length and at a slightly lower magnification.

4. Under the above circumstances you will not have any imaging artifacts
just more contrast and less resolution.

I hope this helps?

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {celikaktas-at-gmail.com}
To: {protrain-at-emcourses.com}
Sent: Friday, September 04, 2009 10:26 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://ns.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yngli-at-ucdavis.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yngli-at-ucdavis.edu
Name: Ying Li

Organization: University of California Davis

Title-Subject: [Filtered] Work parameters for JEOL JEM 2500 SE

Question: Hi, All,
Does anybody knows the below listed values?

for JEOL JEM 2500 SE TEM

Spherical Aberration Cs= mm

Chromatic Aberration Cc= mm

Half Convegence angle =

Scherzer defocus value=

Diameter of objective aperture=

especially for Cs, Cc and Scherzer defocus value,

Can you provide them to me? Or let me know how I can find them?

Thanks!

Ying

Login Host: 169.237.204.132
---------------------------------------------------------------------------

==============================Original Headers==============================
16, 11 -- From zaluzec-at-microscopy.com Sat Sep 5 19:11:12 2009
16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
16, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n860BBWj018174
16, 11 -- for {microscopy-at-microscopy.com} ; Sat, 5 Sep 2009 19:11:12 -0500
16, 11 -- Mime-Version: 1.0
16, 11 -- Message-Id: {p06240809c6c8ae8c41d2-at-[206.69.208.22]}
16, 11 -- Date: Sat, 5 Sep 2009 19:11:11 -0500
16, 11 -- To: microscopy-at-microscopy.com
16, 11 -- From: yngli-at-ucdavis.edu (by way of MicroscopyListserver)
16, 11 -- Subject: viaWWW: Work parameters for JEOL JEM 2500 SE
16, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Mon, 7 Sep 2009 13:14:47 -0500
Subject: [Microscopy] Photo enlarger anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I recall recent posts about give-away photo enlargers, but unfortunately
I deleted them too fast. If anyone (preferably on East Coast) still has
unwanted photo enlarger, either desk-top or free-standing 8"x10" model,
which is collecting dust after the shutdown of a dark-room, and would
like to clear the storage space - please let me know. Can't pay $$$ for
the device, but would gladly come and remove it or pay freight shipping
costs...

Thanks beforehand,

Valery Ray
===================
www.partbeamsystech.com

==============================Original Headers==============================
4, 21 -- From vray-at-partbeamsystech.com Mon Sep 7 13:14:46 2009
4, 21 -- Received: from smtp109.biz.mail.re2.yahoo.com (smtp109.biz.mail.re2.yahoo.com [206.190.53.8])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n87IEk89029329
4, 21 -- for {microscopy-at-microscopy.com} ; Mon, 7 Sep 2009 13:14:46 -0500
4, 21 -- Received: (qmail 68612 invoked from network); 7 Sep 2009 18:14:46 -0000
4, 21 -- Received: from unknown (HELO ?10.0.0.3?) (vray-at-72.85.131.112 with plain)
4, 21 -- by smtp109.biz.mail.re2.yahoo.com with SMTP; 7 Sep 2009 18:14:45 -0000
4, 21 -- X-Yahoo-SMTP: kF2pzF.swBAOVh3sJneOdYK4c6bb5ttJj1IR8A--
4, 21 -- X-YMail-OSG: SJXKMfUVM1m0jKOjYb_mHsipufEob.DA.won80Lt93LX1d3OmArrXc2BdpZqHocLwH1vJZlQzYsByBvEIXW1mq5OxkZQ3PQtcee1M4or.rNfqf4FcXPVex_dtoRPuCblN.MKb6Fl0qmvIKr4jeVrvCG7yFBRpZHimeB7S9lxag_nwIqHulPWwLqrH60A1KSaJ7ZxBZcqh_qPjN8LahAX2ny8jMu1aA4gi__U22sXL9otY5UYXoGuUUso9kLl4YsZCAnbsvQPZLdjNP2ZRF6QEyvD1xxnF.MQmi2vH9NMxnwoGN3CB_sef8GTeHoR1TmieuOKDn83rhzake1OZquRsF0MhrIAMbfMS_kvaCANK5OjP7fK6qFQCU7H2ShYri9iNA--
4, 21 -- X-Yahoo-Newman-Property: ymail-3
4, 21 -- Message-ID: {4AA54D84.3050304-at-partbeamsystech.com}
4, 21 -- Date: Mon, 07 Sep 2009 14:14:28 -0400
4, 21 -- From: Valery Ray {vray-at-partbeamsystech.com}
4, 21 -- Reply-To: vray-at-partbeamsystech.com
4, 21 -- Organization: PBS&T, MEO Engineering Co., Inc.
4, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
4, 21 -- MIME-Version: 1.0
4, 21 -- To: microscopy-at-microscopy.com
4, 21 -- Subject: Photo enlarger anyone?
4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: contact-at-integrityscientific.com
Date: Tue, 8 Sep 2009 10:22:26 -0500
Subject: [Microscopy] Australian j phys paper wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers (especially those down under!)
I am looking for a paper in the Australian Journal of Physics:

Fox, A. G. & Fisher, R. M. Aust. J. Phys. 41, 461-474 (1988)

I find that this journal ceased publication in 2001 and there are no
electronic back issues beyond 1997. Does anyone know how I can get hold
of a copy (preferably electronic)?

Many thanks indeed

Richard Beanland

==============================Original Headers==============================
5, 28 -- From contact-at-integrityscientific.com Tue Sep 8 10:22:26 2009
5, 28 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7])
5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n88FMOoc022764
5, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2009 10:22:25 -0500
5, 28 -- Received: from localhost (localhost [127.0.0.1])
5, 28 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n88FMMmd074721
5, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2009 16:22:22 +0100 (BST)
5, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk
5, 28 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1])
5, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024)
5, 28 -- with LMTP id FiksAI48t5Jr for {microscopy-at-microscopy.com} ;
5, 28 -- Tue, 8 Sep 2009 16:22:17 +0100 (BST)
5, 28 -- Received: from [137.205.164.37] (hosts-137-205-164-37 [137.205.164.37])
5, 28 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n88FM8Am074409
5, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2009 16:22:11 +0100 (BST)
5, 28 -- X-Envelope-From: contact-at-integrityscientific.com
5, 28 -- Message-ID: {4AA6769C.7010203-at-integrityscientific.com}
5, 28 -- Date: Tue, 08 Sep 2009 16:22:04 +0100
5, 28 -- From: Richard Beanland {contact-at-integrityscientific.com}
5, 28 -- Reply-To: contact-at-integrityscientific.com
5, 28 -- Organization: Integrity Scientific Ltd
5, 28 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 28 -- MIME-Version: 1.0
5, 28 -- To: microscopy-at-microscopy.com
5, 28 -- Subject: Australian j phys paper wanted
5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 28 -- Content-Transfer-Encoding: 7bit
5, 28 -- X-DCC-Warwick-Metrics: bluebell; whitelist
==============================End of - Headers==============================




From: contact-at-integrityscientific.com
Date: Tue, 8 Sep 2009 16:23:19 -0500
Subject: [Microscopy] Australian j phys paper found

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks everyone who replied (especially Aaron Barnes), I have the paper now.

Richard


==============================Original Headers==============================
3, 24 -- From contact-at-integrityscientific.com Tue Sep 8 16:23:19 2009
3, 24 -- Received: from em-p01-ob.rzone.de (em-p01-ob.rzone.de [81.169.146.227])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n88LNI0s016160
3, 24 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2009 16:23:19 -0500
3, 24 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBScT3zTB4+xT4zsmzaWssUHC+w==
3, 24 -- X-RZG-CLASS-ID: em01
3, 24 -- Received: from post.webmailer.de (hat.store [192.168.40.68])
3, 24 -- by snori-em-01.store (RZmta 21.0) with ESMTP id a00ea0l88HIaDq
3, 24 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2009 23:23:18 +0200 (MEST)
3, 24 -- Received: (from httpd-at-localhost)
3, 24 -- by post.webmailer.de (8.13.7/8.13.6) id n88LNIAf020955
3, 24 -- for microscopy-at-microscopy.com; Tue, 8 Sep 2009 23:23:18 +0200 (MEST)
3, 24 -- Date: Tue, 8 Sep 2009 23:23:18 +0200 (MEST)
3, 24 -- Message-Id: {200909082123.n88LNIAf020955-at-post.webmailer.de}
3, 24 -- To: microscopy-at-microscopy.com
3, 24 -- From: "Richard Beanland" {contact-at-integrityscientific.com}
3, 24 -- Subject: Australian j phys paper found
3, 24 -- X-Priority: 3
3, 24 -- X-Abuse: 982623 / 86.130.74.141
3, 24 -- X-RZG-MBID: 15cBjAKTcdbsEcTGilfJ4bLrCxUL9rXmAi+LHZqsvAXw2JDlq3IQ2oPu
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain; charset="ISO-8859-1"
3, 24 -- Content-Transfer-Encoding: 8bit
3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n88LNI0s016160
==============================End of - Headers==============================




From: weis183-at-yahoo.fr
Date: Wed, 9 Sep 2009 04:48:45 -0500
Subject: [Microscopy] Re : viaWWW: TEM powder sample prep by ion-milling?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Zhaoxia,

Usually I prepare powders using Ion Slicing technique (JEOL Ion Slicer).
Powders are mixed with G2 Epoxy (The amount of G2 must be as low as possible).
Then I make a sandwich: Glass Slice (100um)/Powder+G2 (200-300um)/Silicon Wafer (500um) (cure at 150°C)
I cut the whole assembly with a wire saw to 2.8mm*800um*700um.
The I polish both sides with diamond laping disks (rough polishing :15um disks) (Minimet Buhler) to obtain a thickness of 100um (sample dimensions are 2.8mm*800um*100um).
Then the Ion slicer makes the job for the final thining.
The quality of the thin slice is usually good enough for BF, DF and HRTEM observations.

This procedure is quite specific to Ion Slicer but it can be used with a PIPS or similar device.

Hope this can help.

Patrick Weisbecker
LCTS
PESSAC-France



----- Message d'origine ----
De : "z.zhou-at-sheffield.ac.uk" {z.zhou-at-sheffield.ac.uk}
À : weis183-at-yahoo.fr
Envoyé le : Vendredi, 4 Septembre 2009, 16h12mn 30s
Objet : [Microscopy] viaWWW: TEM powder sample prep by ion-milling?




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both z.zhou-at-sheffield.ac.uk as well as  the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: z.zhou-at-sheffield.ac.uk
Name: Zhaoxia

Organization: The University of Sheffield, UK

Title-Subject: [Filtered] TEM powder sample prep by ion-milling?

Question: Hello. I have got some LiCoMnO4 powder for TEM/EELS study.
The particles sizes are 0.5-5 microns crushed by pestel and mortar.
The TEM samples prepared by using ultrasonic dispersion turns out to
be too thick for EELS studies.

Some people suggested mix the powder in epoxy and use ion-mill to get
thin area.

Could anyone please advise me with more details about this method? Or
other methods to thin the fine particles?

Thanks,

Zhaoxia Zhou
Dept Engineering Materials,
The University of Sheffield,
Sheffield, UK




  Login Host: 143.167.181.8
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Fri Sep  4 09:07:30 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84E7U6R030877
13, 11 --     for {microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 09:07:30 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240802c6c6cf7fd821-at-[206.69.208.22]}
13, 11 -- Date: Fri, 4 Sep 2009 09:07:29 -0500
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: z.zhou-at-sheffield.ac.uk (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: TEM powder sample prep by ion-milling?
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
30, 25 -- From weis183-at-yahoo.fr Wed Sep 9 04:48:45 2009
30, 25 -- Received: from web24614.mail.ird.yahoo.com (web24614.mail.ird.yahoo.com [212.82.104.171])
30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n899migL028116
30, 25 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 04:48:44 -0500
30, 25 -- Received: (qmail 6051 invoked by uid 60001); 9 Sep 2009 09:48:44 -0000
30, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1252489724; bh=256ghWDdC45+72CzmQ4s6j7wtObqlgpJxnvgbJ4FEHA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=JjUXph1X2fuyEQ0FRH/q6GtvaDJeomWcD2R36ByQ2+szcznjM0pvUbE6YPLck0k51d82Xag55DsFIO2xcEoALMZveIWK3ew8ASf3mu8yHYiX0BUmF2gX+Q3M4oY+s7omsw+wuUPuQ0tyMXPd7JrAATvJOhaO2Z2XT+fI2aXdwr8=
30, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
30, 25 -- s=s1024; d=yahoo.fr;
30, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
30, 25 -- b=KaNuqcMzcJhInnF+jPVCGoiQEz5EIC8+cEyju+cp6+6vwqAdKfOrzNPPHlz/scWafZUKSlOisiGD+7cecSDTFUmI8iHZCkWnDfMynXcuf5CaNElXgYYrCR4z9/oVkSXNpDu2hu86XZQWpsZC9FOzBzj33wNaDsnChrFi2drnKGs=;
30, 25 -- Message-ID: {91372.4895.qm-at-web24614.mail.ird.yahoo.com}
30, 25 -- X-YMail-OSG: JDrHAcsVM1lr2S4oUPvJcJWwfVVGVluWimHIPjwxBhy3l77rMrb0UAu11o8ZN9yhs.buyoS2I4Lz4VrvfbIiOb86BTLZiKwm02bqZyHAl8hRexOWtHpm2jLClmzwgjWO3Z69QYHhznQzollUDR792qo5ZU8tvattiPAmpPCgRo.NqKvkY33pWuJ.7Hp82NPgCsNFzmRRqriik9Nj3ROgX09xYa_ydPB1k37FcbA9SkuJARHfUQJq259LVEctEtCcIxf61ofSY2z6MLFONcGbXWzQdWWlNQVjaxQPamjYrEM-
30, 25 -- Received: from [147.210.80.23] by web24614.mail.ird.yahoo.com via HTTP; Wed, 09 Sep 2009 09:48:43 GMT
30, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
30, 25 -- References: {200909041412.n84ECUma004266-at-ns.microscopy.com}
30, 25 -- Date: Wed, 9 Sep 2009 09:48:43 +0000 (GMT)
30, 25 -- From: Patrick Weisbecker {weis183-at-yahoo.fr}
30, 25 -- Subject: Re : [Microscopy] viaWWW: TEM powder sample prep by ion-milling?
30, 25 -- To: z.zhou-at-sheffield.ac.uk
30, 25 -- Cc: microscopy-at-microscopy.com
30, 25 -- In-Reply-To: {200909041412.n84ECUma004266-at-ns.microscopy.com}
30, 25 -- MIME-Version: 1.0
30, 25 -- Content-Type: text/plain; charset=iso-8859-1
30, 25 -- Content-Transfer-Encoding: 8bit
30, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n899migL028116
==============================End of - Headers==============================




From: mmiralles-at-pi.ac.ae
Date: Wed, 9 Sep 2009 05:27:43 -0500
Subject: [Microscopy] Carbon Coating Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I would like to ask your expertise in the problem I have encountered
with my carbon coating procedure. I tried to coat 7 stubs of rock
fragments all at the same time. However, when I viewed in under the SEM,
3 out of the 4 stubs manifested coating peeling off that looks like
shavings. Would this problem be sample related?

I used our SPI carbon coater with 1.3mm diameter carbon string.

Thanks in advance,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE



==============================Original Headers==============================
7, 29 -- From mmiralles-at-pi.ac.ae Wed Sep 9 05:27:43 2009
7, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n89ARgaS011819
7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 05:27:43 -0500
7, 29 -- X-IronPort-AV: E=Sophos;i="4.44,357,1249243200";
7, 29 -- d="scan'208";a="1510457"
7, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 29 -- by mx1.pi.ac.ae with ESMTP; 09 Sep 2009 14:03:08 +0400
7, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
7, 29 -- Wed, 9 Sep 2009 14:27:27 +0400
7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- charset="us-ascii"
7, 29 -- x-cr-hashedpuzzle: 22A= Aldz CVTO CXLF Crbm CzV4 DlLK Egr4 EhQB EkJQ E9BK I+QA JYf/ J96B KGuV LZ67;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{108DDF47-FCF4-4C21-AD2B-7DBD69D78921};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Wed, 09 Sep 2009 10:27:19 GMT;QwBhAHIAYgBvAG4AIABDAG8AYQB0AGkAbgBnACAAUAByAG8AYgBsAGUAbQA=
7, 29 -- x-cr-puzzleid: {108DDF47-FCF4-4C21-AD2B-7DBD69D78921}
7, 29 -- Content-class: urn:content-classes:message
7, 29 -- Subject: Carbon Coating Problem
7, 29 -- Date: Wed, 9 Sep 2009 14:27:19 +0400
7, 29 -- Message-ID: {D5603421C6303A46883F87E7968F285B078238D8-at-pi-exm.PI.AC.AE}
7, 29 -- X-MS-Has-Attach:
7, 29 -- X-MS-TNEF-Correlator:
7, 29 -- Thread-Topic: Carbon Coating Problem
7, 29 -- Thread-Index: AcoxOBwwedffyoUXRMmw6boEU9E85g==
7, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 29 -- To: {microscopy-at-microscopy.com}
7, 29 -- X-OriginalArrivalTime: 09 Sep 2009 10:27:27.0199 (UTC) FILETIME=[20D12EF0:01CA3138]
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89ARgaS011819
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Wed, 9 Sep 2009 09:18:02 -0500
Subject: [Microscopy] Carbon Coating Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not clear on some of your points. You say you tried to coat 7 stubs
at once, but 3 out of 4 had problems. Is that to say that 5-1/4 had
problems (75% of 7) or that 3 did.

I have seen peeling coatings in other circumstances. It usually had to
do with trying to put down a thick layer for other than microscopic
purposes. The thick layer built up internal stresses that led to
curling.

Did you try to apply the same thickness of coating as normal? You should
have. Adding additional samples does not require extra time or coating
material. The material is there to do the coating whether you have 10
samples or 1 or none. The material will go to coat your chamber if there
are not samples there to receive the coating.

Did the vacuum reach the same level as normal? Our sputter coater has a
good vacuum pump, but we run into cases where samples outgas
significantly and extend the time it takes for the coater to reach a
good vacuum. Using 7 samples instead of 1 could extend the time. I don't
know the SPI coater and whether it works based on vacuum level or time.
Perhaps a poorer vacuum leads to peeling. You might try coating fewer
samples at once or letting the system pump longer and see if the problem
disappears.

We have had some samples whose nature seemed to prevent good coating. We
coated many samples at once, but only a few had the problem. They seemed
to be holding a liquid so that our gold coating did not stay in a layer
but tended to bunch up into large islands. I found that if I pre-pumped
the samples under a higher vacuum - I let them sit in my SEM chamber for
a couple hours - that the samples took a coating better. Apparently
there was some phase that slowly volatized and that extended time under
higher vacuum removed it. It is only rarely a problem, but I have
considered using our high vacuum evaporator to pre-pump the samples if
the problem recurs.

I hope this helps.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae]
Sent: Wednesday, September 09, 2009 5:28 AM
To: wesaia-at-iastate.edu

Hello,

I would like to ask your expertise in the problem I have encountered
with my carbon coating procedure. I tried to coat 7 stubs of rock
fragments all at the same time. However, when I viewed in under the SEM,
3 out of the 4 stubs manifested coating peeling off that looks like
shavings. Would this problem be sample related?

I used our SPI carbon coater with 1.3mm diameter carbon string.

Thanks in advance,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE



==============================Original
Headers==============================
7, 29 -- From mmiralles-at-pi.ac.ae Wed Sep 9 05:27:43 2009
7, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n89ARgaS011819
7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009
05:27:43 -0500
7, 29 -- X-IronPort-AV: E=Sophos;i="4.44,357,1249243200";
7, 29 -- d="scan'208";a="1510457"
7, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 29 -- by mx1.pi.ac.ae with ESMTP; 09 Sep 2009 14:03:08 +0400
7, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by
PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
7, 29 -- Wed, 9 Sep 2009 14:27:27 +0400
7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- charset="us-ascii"
7, 29 -- x-cr-hashedpuzzle: 22A= Aldz CVTO CXLF Crbm CzV4 DlLK Egr4 EhQB
EkJQ E9BK I+QA JYf/ J96B KGuV
LZ67;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8Ab
QA=;Sosha1_v1;7;{108DDF47-FCF4-4C21-AD2B-7DBD69D78921};bQBtAGkAcgBhAGwAb
ABlAHMAQABwAGkALgBhAGMALgBhAGUA;Wed, 09 Sep 2009 10:27:19
GMT;QwBhAHIAYgBvAG4AIABDAG8AYQB0AGkAbgBnACAAUAByAG8AYgBsAGUAbQA=
7, 29 -- x-cr-puzzleid: {108DDF47-FCF4-4C21-AD2B-7DBD69D78921}
7, 29 -- Content-class: urn:content-classes:message
7, 29 -- Subject: Carbon Coating Problem
7, 29 -- Date: Wed, 9 Sep 2009 14:27:19 +0400
7, 29 -- Message-ID:
{D5603421C6303A46883F87E7968F285B078238D8-at-pi-exm.PI.AC.AE}
7, 29 -- X-MS-Has-Attach:
7, 29 -- X-MS-TNEF-Correlator:
7, 29 -- Thread-Topic: Carbon Coating Problem
7, 29 -- Thread-Index: AcoxOBwwedffyoUXRMmw6boEU9E85g==
7, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 29 -- To: {microscopy-at-microscopy.com}
7, 29 -- X-OriginalArrivalTime: 09 Sep 2009 10:27:27.0199 (UTC)
FILETIME=[20D12EF0:01CA3138]
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n89ARgaS011819
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 37 -- From wesaia-at-iastate.edu Wed Sep 9 09:18:02 2009
20, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
20, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n89EI153007399
20, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 09:18:01 -0500
20, 37 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
20, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n89EI062003102;
20, 37 -- Wed, 9 Sep 2009 09:18:00 -0500
20, 37 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
20, 37 -- id 0b0c_948971b6_9d4b_11de_a8f3_001372578af6;
20, 37 -- Wed, 09 Sep 2009 09:18:00 -0500
20, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
20, 37 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n89EHwYj029563;
20, 37 -- Wed, 9 Sep 2009 09:17:59 -0500
20, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
20, 37 -- Wed, 9 Sep 2009 09:17:58 -0500
20, 37 -- Content-class: urn:content-classes:message
20, 37 -- MIME-Version: 1.0
20, 37 -- Content-Type: text/plain;
20, 37 -- charset="US-ASCII"
20, 37 -- Subject: RE: [Microscopy] Carbon Coating Problem
20, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 37 -- Date: Wed, 9 Sep 2009 09:20:43 -0500
20, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72704273D70-at-maire.eng.iastate.edu}
20, 37 -- In-Reply-To: {200909091028.n89ASERx012822-at-ns.microscopy.com}
20, 37 -- X-MS-Has-Attach:
20, 37 -- X-MS-TNEF-Correlator:
20, 37 -- Thread-Topic: [Microscopy] Carbon Coating Problem
20, 37 -- Thread-Index: AcoxOD6645Eghq7aQHOOmag1wxghdgAHR9mA
20, 37 -- References: {200909091028.n89ASERx012822-at-ns.microscopy.com}
20, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
20, 37 -- To: {mmiralles-at-pi.ac.ae}
20, 37 -- Cc: {Microscopy-at-microscopy.com}
20, 37 -- X-OriginalArrivalTime: 09 Sep 2009 14:17:58.0191 (UTC) FILETIME=[54BB13F0:01CA3158]
20, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.9.9.140334
20, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0'
20, 37 -- Content-Transfer-Encoding: 8bit
20, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89EI153007399
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Wed, 9 Sep 2009 11:59:57 -0500
Subject: [Microscopy] Carbon Coating Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Adhesion problems for coatings are typically a surface preparation problem.
I would double check your cleaning procedures for your samples prior to
coating. Some coatings are not compatible with some surfaces. Are the good
samples and poor samples the same material? Often, you can change the
surface properties with a plasma preparation. For example, polymer surfaces
are often treated with an Argon plasma to modify the wetting characteristics
of the film going down on the surface.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae]
Sent: Wednesday, September 09, 2009 3:34 AM
To: swalck-at-southbaytech.com

Hello,

I would like to ask your expertise in the problem I have encountered
with my carbon coating procedure. I tried to coat 7 stubs of rock
fragments all at the same time. However, when I viewed in under the SEM,
3 out of the 4 stubs manifested coating peeling off that looks like
shavings. Would this problem be sample related?

I used our SPI carbon coater with 1.3mm diameter carbon string.

Thanks in advance,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE



==============================Original Headers==============================
7, 29 -- From mmiralles-at-pi.ac.ae Wed Sep 9 05:27:43 2009
7, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n89ARgaS011819
7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 05:27:43
-0500
7, 29 -- X-IronPort-AV: E=Sophos;i="4.44,357,1249243200";
7, 29 -- d="scan'208";a="1510457"
7, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 29 -- by mx1.pi.ac.ae with ESMTP; 09 Sep 2009 14:03:08 +0400
7, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE
with Microsoft SMTPSVC(6.0.3790.3959);
7, 29 -- Wed, 9 Sep 2009 14:27:27 +0400
7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- charset="us-ascii"
7, 29 -- x-cr-hashedpuzzle: 22A= Aldz CVTO CXLF Crbm CzV4 DlLK Egr4 EhQB
EkJQ E9BK I+QA JYf/ J96B KGuV
LZ67;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;
Sosha1_v1;7;{108DDF47-FCF4-4C21-AD2B-7DBD69D78921};bQBtAGkAcgBhAGwAbABlAHMAQ
ABwAGkALgBhAGMALgBhAGUA;Wed, 09 Sep 2009 10:27:19
GMT;QwBhAHIAYgBvAG4AIABDAG8AYQB0AGkAbgBnACAAUAByAG8AYgBsAGUAbQA=
7, 29 -- x-cr-puzzleid: {108DDF47-FCF4-4C21-AD2B-7DBD69D78921}
7, 29 -- Content-class: urn:content-classes:message
7, 29 -- Subject: Carbon Coating Problem
7, 29 -- Date: Wed, 9 Sep 2009 14:27:19 +0400
7, 29 -- Message-ID:
{D5603421C6303A46883F87E7968F285B078238D8-at-pi-exm.PI.AC.AE}
7, 29 -- X-MS-Has-Attach:
7, 29 -- X-MS-TNEF-Correlator:
7, 29 -- Thread-Topic: Carbon Coating Problem
7, 29 -- Thread-Index: AcoxOBwwedffyoUXRMmw6boEU9E85g==
7, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 29 -- To: {microscopy-at-microscopy.com}
7, 29 -- X-OriginalArrivalTime: 09 Sep 2009 10:27:27.0199 (UTC)
FILETIME=[20D12EF0:01CA3138]
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n89ARgaS011819
==============================End of - Headers==============================





==============================Original Headers==============================
19, 31 -- From swalck-at-southbaytech.com Wed Sep 9 11:59:56 2009
19, 31 -- Received: from n66.bullet.mail.sp1.yahoo.com (n66.bullet.mail.sp1.yahoo.com [98.136.44.50])
19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n89GxuNi026527
19, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 11:59:56 -0500
19, 31 -- Received: from [216.252.122.219] by n66.bullet.mail.sp1.yahoo.com with NNFMP; 09 Sep 2009 16:58:45 -0000
19, 31 -- Received: from [68.142.200.221] by t4.bullet.sp1.yahoo.com with NNFMP; 09 Sep 2009 16:58:45 -0000
19, 31 -- Received: from [68.142.201.70] by t9.bullet.mud.yahoo.com with NNFMP; 09 Sep 2009 16:58:45 -0000
19, 31 -- Received: from [127.0.0.1] by omp422.mail.mud.yahoo.com with NNFMP; 09 Sep 2009 16:58:45 -0000
19, 31 -- X-Yahoo-Newman-Id: 402415.35303.bm-at-omp422.mail.mud.yahoo.com
19, 31 -- Received: (qmail 63987 invoked from network); 9 Sep 2009 16:58:44 -0000
19, 31 -- Received: from unknown (HELO SWALCKD1) (swalck-at-99.154.21.201 with login)
19, 31 -- by smtp112.plus.mail.sp1.yahoo.com with SMTP; 9 Sep 2009 16:58:44 -0000
19, 31 -- X-Yahoo-SMTP: rolQR_SswBBVziZtd9khRtiR2zI_imwDSS008k3o3DaS
19, 31 -- X-YMail-OSG: snnjrK4VM1kXubeNb3yfCgf6TUxGcKJCOO1pDzlFXdNCnc_uPSLtonW7x_rJM73ehEoc6ZDKaG3CDYh4P2VNo80Dftm2BVQNg7zdVYBVAC2yR7k6YGlvRxRXnDI_T.zvlmtJRxbxbRDRQLWuoT0.22PCYquYVXtsjlsnnv_KklUbEp_Lm18QQNUoWsJvTXVSiqtLqyaiSLoUh97baRqiJwXyeuRiLmea7FHlgBf6DRz4_wKBHbE-
19, 31 -- X-Yahoo-Newman-Property: ymail-3
19, 31 -- From: "Scott Walck" {swalck-at-southbaytech.com}
19, 31 -- To: {mmiralles-at-pi.ac.ae}
19, 31 -- Cc: {Microscopy-at-microscopy.com}
19, 31 -- References: {200909091033.n89AXnU8025171-at-ns.microscopy.com}
19, 31 -- In-Reply-To: {200909091033.n89AXnU8025171-at-ns.microscopy.com}
19, 31 -- Subject: RE: [Microscopy] Carbon Coating Problem
19, 31 -- Date: Wed, 9 Sep 2009 10:00:41 -0700
19, 31 -- Message-ID: {012701ca316f$10a65eb0$31f31c10$-at-com}
19, 31 -- MIME-Version: 1.0
19, 31 -- Content-Type: text/plain;
19, 31 -- charset="iso-8859-1"
19, 31 -- X-Mailer: Microsoft Office Outlook 12.0
19, 31 -- Content-language: en-us
19, 31 -- Thread-index: AcoxOQT7pf285hskS2eOjWyWjWzgbgANYqrw
19, 31 -- Content-Transfer-Encoding: 8bit
19, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89GxuNi026527
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Wed, 9 Sep 2009 12:23:14 -0500
Subject: [Microscopy] Carbon Coating Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I ran into this problem after coating the inside of my bell jar with a releasing agent. Too much carbon buildup and it would peel right off during flashover falling on the samples below. About every third coating now I hit the bell jar with a can of air to blow off any peeling film and have not had problems since.


Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891


-----Original Message-----
X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae]
Sent: Wednesday, September 09, 2009 6:29 AM
To: Whittaker, Scott

Hello,

I would like to ask your expertise in the problem I have encountered
with my carbon coating procedure. I tried to coat 7 stubs of rock
fragments all at the same time. However, when I viewed in under the SEM,
3 out of the 4 stubs manifested coating peeling off that looks like
shavings. Would this problem be sample related?

I used our SPI carbon coater with 1.3mm diameter carbon string.

Thanks in advance,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE



==============================Original Headers==============================
7, 29 -- From mmiralles-at-pi.ac.ae Wed Sep 9 05:27:43 2009
7, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n89ARgaS011819
7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 05:27:43 -0500
7, 29 -- X-IronPort-AV: E=Sophos;i="4.44,357,1249243200";
7, 29 -- d="scan'208";a="1510457"
7, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 29 -- by mx1.pi.ac.ae with ESMTP; 09 Sep 2009 14:03:08 +0400
7, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
7, 29 -- Wed, 9 Sep 2009 14:27:27 +0400
7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- charset="us-ascii"
7, 29 -- x-cr-hashedpuzzle: 22A= Aldz CVTO CXLF Crbm CzV4 DlLK Egr4 EhQB EkJQ E9BK I+QA JYf/ J96B KGuV LZ67;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{108DDF47-FCF4-4C21-AD2B-7DBD69D78921};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Wed, 09 Sep 2009 10:27:19 GMT;QwBhAHIAYgBvAG4AIABDAG8AYQB0AGkAbgBnACAAUAByAG8AYgBsAGUAbQA=
7, 29 -- x-cr-puzzleid: {108DDF47-FCF4-4C21-AD2B-7DBD69D78921}
7, 29 -- Content-class: urn:content-classes:message
7, 29 -- Subject: Carbon Coating Problem
7, 29 -- Date: Wed, 9 Sep 2009 14:27:19 +0400
7, 29 -- Message-ID: {D5603421C6303A46883F87E7968F285B078238D8-at-pi-exm.PI.AC.AE}
7, 29 -- X-MS-Has-Attach:
7, 29 -- X-MS-TNEF-Correlator:
7, 29 -- Thread-Topic: Carbon Coating Problem
7, 29 -- Thread-Index: AcoxOBwwedffyoUXRMmw6boEU9E85g==
7, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 29 -- To: {microscopy-at-microscopy.com}
7, 29 -- X-OriginalArrivalTime: 09 Sep 2009 10:27:27.0199 (UTC) FILETIME=[20D12EF0:01CA3138]
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89ARgaS011819
==============================End of - Headers==============================


==============================Original Headers==============================
17, 30 -- From WHITTAKS-at-si.edu Wed Sep 9 12:23:13 2009
17, 30 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178])
17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n89HNDmj008740
17, 30 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 12:23:13 -0500
17, 30 -- Received: from SI-MSEHUB-N02.US.SINET.SI.EDU (si-msehub-n02.us.sinet.si.edu [160.111.49.126])
17, 30 -- by si-mailout04.si.edu (Postfix) with ESMTP id 7477AA9D2;
17, 30 -- Wed, 9 Sep 2009 13:18:59 -0400 (EDT)
17, 30 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::f1ea:a780:6df5:c7bd]) by
17, 30 -- SI-MSEHUB-N02.US.SINET.SI.EDU ([fe80::dfb:a2f1:b0f7:9c85%11]) with mapi; Wed,
17, 30 -- 9 Sep 2009 13:23:11 -0400
17, 30 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
17, 30 -- To: "'mmiralles-at-pi.ac.ae'" {mmiralles-at-pi.ac.ae} ,
17, 30 -- "'microscopy-at-microscopy.com'"
17, 30 -- {microscopy-at-microscopy.com}
17, 30 -- Date: Wed, 9 Sep 2009 13:23:10 -0400
17, 30 -- Subject: RE: [Microscopy] Carbon Coating Problem
17, 30 -- Thread-Topic: [Microscopy] Carbon Coating Problem
17, 30 -- Thread-Index: AcoxOFqr08Lwif0XS7qN8uERysonTgAOcyEw
17, 30 -- Message-ID: {038C04650A93A14381E944A473695F618C68720207-at-SI-MSEV03.US.SINET.SI.EDU}
17, 30 -- References: {200909091029.n89AT270013996-at-ns.microscopy.com}
17, 30 -- In-Reply-To: {200909091029.n89AT270013996-at-ns.microscopy.com}
17, 30 -- Accept-Language: en-US
17, 30 -- Content-Language: en-US
17, 30 -- X-MS-Has-Attach:
17, 30 -- X-MS-TNEF-Correlator:
17, 30 -- acceptlanguage: en-US
17, 30 -- Content-Type: text/plain; charset="iso-8859-1"
17, 30 -- MIME-Version: 1.0
17, 30 -- Content-Transfer-Encoding: 8bit
17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89HNDmj008740
==============================End of - Headers==============================




From: donald.gibbon-at-matcoinc.com
Date: Wed, 9 Sep 2009 14:50:35 -0500
Subject: [Microscopy] Re : viaWWW: TEM powder sample prep by ion-milling?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That ion slicer is really slick, if it works as well as the video on the JEOL site says it does. But if you haven't got such lovely resources, any "old-fashioned" ion milling device should also get the job done at some level. However, I'd be surprised if some of the particles produced by grinding in a mortar and pestle would not be transparent to 100-200Kv electrons, simply dispersed onto a carbon-covered grid. The easy way to identify the transparent ones is to do dark-field microscopy. Produce a powder pattern and search around it with the objective aperture, looking for the particles that light up. Those are the ones that are transparent, on which you can do more careful studies.

Donald L. Gibbon
MATCO Services
Pittsburgh, PA

-----Original Message-----
X-from: weis183-at-yahoo.fr [mailto:weis183-at-yahoo.fr]
Sent: Wednesday, September 09, 2009 5:58 AM
To: Gibbon, Donald L.

Hi Zhaoxia,

Usually I prepare powders using Ion Slicing technique (JEOL Ion Slicer).
Powders are mixed with G2 Epoxy (The amount of G2 must be as low as possible).
Then I make a sandwich: Glass Slice (100um)/Powder+G2 (200-300um)/Silicon Wafer (500um) (cure at 150°C)
I cut the whole assembly with a wire saw to 2.8mm*800um*700um.
The I polish both sides with diamond laping disks (rough polishing :15um disks) (Minimet Buhler) to obtain a thickness of 100um (sample dimensions are 2.8mm*800um*100um).
Then the Ion slicer makes the job for the final thining.
The quality of the thin slice is usually good enough for BF, DF and HRTEM observations.

This procedure is quite specific to Ion Slicer but it can be used with a PIPS or similar device.

Hope this can help.

Patrick Weisbecker
LCTS
PESSAC-France



----- Message d'origine ----
De : "z.zhou-at-sheffield.ac.uk" {z.zhou-at-sheffield.ac.uk}
À : weis183-at-yahoo.fr
Envoyé le : Vendredi, 4 Septembre 2009, 16h12mn 30s
Objet : [Microscopy] viaWWW: TEM powder sample prep by ion-milling?




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both z.zhou-at-sheffield.ac.uk as well as  the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: z.zhou-at-sheffield.ac.uk
Name: Zhaoxia

Organization: The University of Sheffield, UK

Title-Subject: [Filtered] TEM powder sample prep by ion-milling?

Question: Hello. I have got some LiCoMnO4 powder for TEM/EELS study.
The particles sizes are 0.5-5 microns crushed by pestel and mortar.
The TEM samples prepared by using ultrasonic dispersion turns out to
be too thick for EELS studies.

Some people suggested mix the powder in epoxy and use ion-mill to get
thin area.

Could anyone please advise me with more details about this method? Or
other methods to thin the fine particles?

Thanks,

Zhaoxia Zhou
Dept Engineering Materials,
The University of Sheffield,
Sheffield, UK




  Login Host: 143.167.181.8
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Fri Sep  4 09:07:30 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n84E7U6R030877
13, 11 --     for {microscopy-at-microscopy.com} ; Fri, 4 Sep 2009 09:07:30 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240802c6c6cf7fd821-at-[206.69.208.22]}
13, 11 -- Date: Fri, 4 Sep 2009 09:07:29 -0500
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: z.zhou-at-sheffield.ac.uk (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: TEM powder sample prep by ion-milling?
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
30, 25 -- From weis183-at-yahoo.fr Wed Sep 9 04:48:45 2009
30, 25 -- Received: from web24614.mail.ird.yahoo.com (web24614.mail.ird.yahoo.com [212.82.104.171])
30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n899migL028116
30, 25 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 04:48:44 -0500
30, 25 -- Received: (qmail 6051 invoked by uid 60001); 9 Sep 2009 09:48:44 -0000
30, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1252489724; bh=256ghWDdC45+72CzmQ4s6j7wtObqlgpJxnvgbJ4FEHA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=JjUXph1X2fuyEQ0FRH/q6GtvaDJeomWcD2R36ByQ2+szcznjM0pvUbE6YPLck0k51d82Xag55DsFIO2xcEoALMZveIWK3ew8ASf3mu8yHYiX0BUmF2gX+Q3M4oY+s7omsw+wuUPuQ0tyMXPd7JrAATvJOhaO2Z2XT+fI2aXdwr8=
30, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
30, 25 -- s=s1024; d=yahoo.fr;
30, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
30, 25 -- b=KaNuqcMzcJhInnF+jPVCGoiQEz5EIC8+cEyju+cp6+6vwqAdKfOrzNPPHlz/scWafZUKSlOisiGD+7cecSDTFUmI8iHZCkWnDfMynXcuf5CaNElXgYYrCR4z9/oVkSXNpDu2hu86XZQWpsZC9FOzBzj33wNaDsnChrFi2drnKGs=;
30, 25 -- Message-ID: {91372.4895.qm-at-web24614.mail.ird.yahoo.com}
30, 25 -- X-YMail-OSG: JDrHAcsVM1lr2S4oUPvJcJWwfVVGVluWimHIPjwxBhy3l77rMrb0UAu11o8ZN9yhs.buyoS2I4Lz4VrvfbIiOb86BTLZiKwm02bqZyHAl8hRexOWtHpm2jLClmzwgjWO3Z69QYHhznQzollUDR792qo5ZU8tvattiPAmpPCgRo.NqKvkY33pWuJ.7Hp82NPgCsNFzmRRqriik9Nj3ROgX09xYa_ydPB1k37FcbA9SkuJARHfUQJq259LVEctEtCcIxf61ofSY2z6MLFONcGbXWzQdWWlNQVjaxQPamjYrEM-
30, 25 -- Received: from [147.210.80.23] by web24614.mail.ird.yahoo.com via HTTP; Wed, 09 Sep 2009 09:48:43 GMT
30, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
30, 25 -- References: {200909041412.n84ECUma004266-at-ns.microscopy.com}
30, 25 -- Date: Wed, 9 Sep 2009 09:48:43 +0000 (GMT)
30, 25 -- From: Patrick Weisbecker {weis183-at-yahoo.fr}
30, 25 -- Subject: Re : [Microscopy] viaWWW: TEM powder sample prep by ion-milling?
30, 25 -- To: z.zhou-at-sheffield.ac.uk
30, 25 -- Cc: microscopy-at-microscopy.com
30, 25 -- In-Reply-To: {200909041412.n84ECUma004266-at-ns.microscopy.com}
30, 25 -- MIME-Version: 1.0
30, 25 -- Content-Type: text/plain; charset=iso-8859-1
30, 25 -- Content-Transfer-Encoding: 8bit
30, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n899migL028116
==============================End of - Headers==============================

The information contained in this E-mail message and the documents
accompanying this message are privileged and confidential, and may be protected
from disclosure. Please be aware that any use, printing, copying, disclosure or
dissemination of this communication may be subject to legal restriction or
sanction. If you think that you have received this E-mail message in error, please
reply to the sender.

For more information about Valmont Industries, Inc., please visit our web site at
www.valmont.com


==============================Original Headers==============================
40, 27 -- From donald.gibbon-at-matcoinc.com Wed Sep 9 14:50:34 2009
40, 27 -- Received: from smtp1.valmont.com (smtp1.valmont.com [72.165.239.151])
40, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n89JoYT3027179
40, 27 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2009 14:50:34 -0500
40, 27 -- X-TM-IMSS-Message-ID: {399f0b550010a26b-at-smtp1.valmont.com}
40, 27 -- Received: from valmailbh1.na.valmont.com ([172.16.0.51]) by smtp1.valmont.com ([192.168.123.151]) with ESMTP (TREND IMSS SMTP Service 7.0) id 399f0b550010a26b ; Wed, 9 Sep 2009 14:50:24 -0500
40, 27 -- Received: from valmail.na.valmont.com ([172.16.1.35]) by valmailbh1.na.valmont.com with Microsoft SMTPSVC(6.0.3790.3959);
40, 27 -- Wed, 9 Sep 2009 14:50:18 -0500
40, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
40, 27 -- Content-class: urn:content-classes:message
40, 27 -- MIME-Version: 1.0
40, 27 -- Content-Type: text/plain;
40, 27 -- charset="iso-8859-1"
40, 27 -- Subject: RE: [Microscopy] Re : viaWWW: TEM powder sample prep by ion-milling?
40, 27 -- Date: Wed, 9 Sep 2009 14:49:29 -0500
40, 27 -- Message-ID: {AE9049B5C6BD064BA3F0B94A1F985BA7DE528A-at-valmail.na.valmont.com}
40, 27 -- In-Reply-To: {200909090957.n899vphO009201-at-ns.microscopy.com}
40, 27 -- X-MS-Has-Attach:
40, 27 -- X-MS-TNEF-Correlator:
40, 27 -- Thread-Topic: [Microscopy] Re : viaWWW: TEM powder sample prep by ion-milling?
40, 27 -- Thread-Index: AcoxM/8ExHJg3lReQo6zpztFi2x5ZgAUX3rw
40, 27 -- References: {200909090957.n899vphO009201-at-ns.microscopy.com}
40, 27 -- From: "Gibbon, Donald L." {donald.gibbon-at-matcoinc.com}
40, 27 -- To: {microscopy-at-microscopy.com}
40, 27 -- X-OriginalArrivalTime: 09 Sep 2009 19:50:18.0037 (UTC) FILETIME=[C1CE1E50:01CA3186]
40, 27 -- Content-Transfer-Encoding: 8bit
40, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n89JoYT3027179
==============================End of - Headers==============================




From: mmiralles-at-pi.ac.ae
Date: Thu, 10 Sep 2009 00:57:51 -0500
Subject: [Microscopy] Carbon Coating Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again to Warren & all,

Sorry, I meant to say, 3 out of 7 had problems. The four others turned
out perfectly okay. I have applied the same amount of carbon as I
usually use on other samples and there was also no significant increase
on the time it took to reach a good vacuum. Anyhow, will try washing off
and drying the samples as most of you suggested and play around with the
amount of sample per batch of coating cycle. I would also look and see
any trend with the sample composition of the good ones versus the bad
ones.

I cannot try the suggestions that use other equipments aside from the
ones I mentioned, as these two are our only workhorses in our SEM lab.
However, I appreciate that you and other forumers mentioned other
equipments that we could look into and perhaps add in the future.

Will try to have fun now... thanks so much!
Melina

-----Original Message-----
X-from: Straszheim, Warren E [M S E] [mailto:wesaia-at-iastate.edu]
Sent: Wednesday, September 09, 2009 6:21 PM
To: Melina Miralles
Cc: Microscopy-at-microscopy.com

Hello,

I would like to ask your expertise in the problem I have encountered
with my carbon coating procedure. I tried to coat 7 stubs of rock
fragments all at the same time. However, when I viewed in under the SEM,
3 out of the 4 stubs manifested coating peeling off that looks like
shavings. Would this problem be sample related?

I used our SPI carbon coater with 1.3mm diameter carbon string.

Thanks in advance,

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE





==============================Original Headers==============================
25, 30 -- From mmiralles-at-pi.ac.ae Thu Sep 10 00:57:51 2009
25, 30 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8A5vnRv025881
25, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 00:57:50 -0500
25, 30 -- X-IronPort-AV: E=Sophos;i="4.44,362,1249243200";
25, 30 -- d="scan'208";a="1513917"
25, 30 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
25, 30 -- by mx1.pi.ac.ae with ESMTP; 10 Sep 2009 09:33:14 +0400
25, 30 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
25, 30 -- Thu, 10 Sep 2009 09:57:35 +0400
25, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
25, 30 -- Content-class: urn:content-classes:message
25, 30 -- MIME-Version: 1.0
25, 30 -- Content-Type: text/plain;
25, 30 -- charset="us-ascii"
25, 30 -- Subject: RE: [Microscopy] Carbon Coating Problem
25, 30 -- Date: Thu, 10 Sep 2009 09:57:33 +0400
25, 30 -- Message-ID: {D5603421C6303A46883F87E7968F285B07823CEF-at-pi-exm.PI.AC.AE}
25, 30 -- In-reply-to: {16A330AC32056A40B32842EC4BB8D72704273D70-at-maire.eng.iastate.edu}
25, 30 -- X-MS-Has-Attach:
25, 30 -- X-MS-TNEF-Correlator:
25, 30 -- Thread-Topic: [Microscopy] Carbon Coating Problem
25, 30 -- Thread-Index: AcoxOD6645Eghq7aQHOOmag1wxghdgAHR9mAACCQ12A=
25, 30 -- References: {200909091028.n89ASERx012822-at-ns.microscopy.com} {16A330AC32056A40B32842EC4BB8D72704273D70-at-maire.eng.iastate.edu}
25, 30 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
25, 30 -- To: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
25, 30 -- Cc: {Microscopy-at-microscopy.com}
25, 30 -- X-OriginalArrivalTime: 10 Sep 2009 05:57:35.0994 (UTC) FILETIME=[98851DA0:01CA31DB]
25, 30 -- Content-Transfer-Encoding: 8bit
25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8A5vnRv025881
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 10 Sep 2009 06:23:27 -0500
Subject: [Microscopy] viaWWW: TEM powder sample prep by ion-milling?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

I won't directly answer your question, but I will offer you a solution which, if it works, will be much easier than any other. With the development of technology people tend more and more to use brute force instead of smart design.
In your crush mix, I would guess that you have a continuum of diameters between 0 and 5 µm, with a majority in the µm range as you stated.
I wouldn't be surprised that some particles are 100nm in diameter and under. Of course they are few, but for TEM work one needs really few material.
You may try resuspending the powder in ethanol and centrifuging at different speed/time (perhaps just sedimenting works, it depends on the density of the particles), and drying one drop of supernatant on a film-grid. This way you'll select only the finest of the particles. If the particles are too disperse, just let the supernatant evaporate!
If this does not work, then use resin embedding, ion-milling and brute force :-)

Regards,

Stephane

 


----- Original Message ----
X-from: "z.zhou-at-sheffield.ac.uk" {z.zhou-at-sheffield.ac.uk}
To: nizets2-at-yahoo.com
Sent: Friday, September 4, 2009 4:12:56 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both z.zhou-at-sheffield.ac.uk as well as  the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: z.zhou-at-sheffield.ac.uk
Name: Zhaoxia

Organization: The University of Sheffield, UK

Title-Subject: [Filtered] TEM powder sample prep by ion-milling?

Question: Hello. I have got some LiCoMnO4 powder for TEM/EELS study.
The particles sizes are 0.5-5 microns crushed by pestel and mortar.
The TEM samples prepared by using ultrasonic dispersion turns out to
be too thick for EELS studies.

Some people suggested mix the powder in epoxy and use ion-mill to get
thin area.

Could anyone please advise me with more details about this method? Or
other methods to thin the fine particles?

Thanks,

Zhaoxia Zhou
Dept Engineering Materials,
The University of Sheffield,
Sheffield, UK





==============================Original Headers==============================
23, 22 -- From nizets2-at-yahoo.com Thu Sep 10 06:23:27 2009
23, 22 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225])
23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8ABNQnl019751
23, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 06:23:26 -0500
23, 22 -- Received: (qmail 48002 invoked by uid 60001); 10 Sep 2009 11:23:26 -0000
23, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1252581806; bh=frvQf5RzUzUPLHVeHrxcu8+45uhLet+PzN/xd3fExqU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0GcilQvaIy9OdjDIEPEyKEyyZWfRQKu/yhneB8EpjAUYu8L5AqIRr+565+D/HL/U9KXkCRIEbvDQ/wfMY92S8CTL6DidpWMSL4nvdLaVgqSakcLOO5MjhpKWiSjzxeqwN8A/YeuUQQVQHLDPo+irbGDK6n750ubikSXpk136JXQ=
23, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 22 -- s=s1024; d=yahoo.com;
23, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 22 -- b=sYEzKEy9IG9bV1Nj4rulxBLAZV5AzUoqIyXJc9gRFPrqoywdygd+9jhOGXzeXGGY4m1726+CiEjuVHA+jzfzymba3Mtk3O8aOUGpDSGtjHbv+FwTlUtm9QS3LyaZ0xidvzmjngmb1AmsU2z9yNOM0VLCpsuzsVVIISHYhmwrzHw=;
23, 22 -- Message-ID: {34822.47499.qm-at-web110802.mail.gq1.yahoo.com}
23, 22 -- X-YMail-OSG: CO6_PMQVM1lDrJC.4Vtg74weN1IEJ3v3ITU0Ltgpnyo1QmejxxedyFTptND3afAmRueVCmYuv5EQScekPUy2BRGXkSDN6JgZbmaQxtbJr.TqapZpszOgy6tsOtCvfpw28ZYgw31jTw6j.KJo7_J3iYZANl1tQ0zdMMVOMNTveiEhL0D11G2IVFt4EP3R5A_qSSodAFy4SOiH4jUiX4y1rszQyLFeXfOccrycQbxdhOlhtuczMa7l2hmh6HdlpIfaIqj3WIXveuseMtAauy.Ni0.ZGkJkC1as1bREzhe4nvIN1gn6AF9msON4DBetnkRYdUs_s_OfURyT
23, 22 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Thu, 10 Sep 2009 04:23:25 PDT
23, 22 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.347.2
23, 22 -- Date: Thu, 10 Sep 2009 04:23:25 -0700 (PDT)
23, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 22 -- Subject: Re: [Microscopy] viaWWW: TEM powder sample prep by ion-milling?
23, 22 -- To: microscopy-at-microscopy.com
23, 22 -- MIME-Version: 1.0
23, 22 -- Content-Type: text/plain; charset=iso-8859-1
23, 22 -- Content-Transfer-Encoding: 8bit
23, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8ABNQnl019751
==============================End of - Headers==============================




From: weis183-at-yahoo.fr
Date: Thu, 10 Sep 2009 07:56:32 -0500
Subject: [Microscopy] viaWWW: TEM powder sample prep by ion-milling?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I usually avoid crushing methods. Ok it's easy and quick but information are often limited, crushing induce mechanical damages and thin areas are often not thin enough for HRTEM or EELS works. It's often difficult to see the original grain shape and distance to the surface is difficult to evaluate.
Moreover some materials tend to make "sheets" or brake along preferential direction and thus are not observable in all directions.
In fact this is the crushing that is the brute force...

For the question fo Zhaoxia, FIB could also be an (expensive) mean to obtain thin slices. Thickness is constant and thus ideal for EELS analysis.

Patrick



----- Message d'origine ----
De : "nizets2-at-yahoo.com" {nizets2-at-yahoo.com}
À : weis183-at-yahoo.fr
Envoyé le : Jeudi, 10 Septembre 2009, 13h27mn 49s
Objet : [Microscopy] Re: viaWWW: TEM powder sample prep by ion-milling?




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

Hi!

I won't directly answer your question, but I will offer you a solution which, if it works, will be much easier than any other. With the development of technology people tend more and more to use brute force instead of smart design.
In your crush mix, I would guess that you have a continuum of diameters between 0 and 5 µm, with a majority in the µm range as you stated.
I wouldn't be surprised that some particles are 100nm in diameter and under. Of course they are few, but for TEM work one needs really few material.
You may try resuspending the powder in ethanol and centrifuging at different speed/time (perhaps just sedimenting works, it depends on the density of the particles), and drying one drop of supernatant on a film-grid. This way you'll select only the finest of the particles. If the particles are too disperse, just let the supernatant evaporate!
If this does not work, then use resin embedding, ion-milling and brute force :-)

Regards,

Stephane

 


----- Original Message ----
X-from: "z.zhou-at-sheffield.ac.uk" {z.zhou-at-sheffield.ac.uk}
To: nizets2-at-yahoo.com
Sent: Friday, September 4, 2009 4:12:56 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both z.zhou-at-sheffield.ac.uk as well as  the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: z.zhou-at-sheffield.ac.uk
Name: Zhaoxia

Organization: The University of Sheffield, UK

Title-Subject: [Filtered] TEM powder sample prep by ion-milling?

Question: Hello. I have got some LiCoMnO4 powder for TEM/EELS study.
The particles sizes are 0.5-5 microns crushed by pestel and mortar.
The TEM samples prepared by using ultrasonic dispersion turns out to
be too thick for EELS studies.

Some people suggested mix the powder in epoxy and use ion-mill to get
thin area.

Could anyone please advise me with more details about this method? Or
other methods to thin the fine particles?

Thanks,

Zhaoxia Zhou
Dept Engineering Materials,
The University of Sheffield,
Sheffield, UK


     


==============================Original Headers==============================
23, 22 -- From nizets2-at-yahoo.com Thu Sep 10 06:23:27 2009
23, 22 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225])
23, 22 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8ABNQnl019751
23, 22 --     for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 06:23:26 -0500
23, 22 -- Received: (qmail 48002 invoked by uid 60001); 10 Sep 2009 11:23:26 -0000
23, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1252581806; bh=frvQf5RzUzUPLHVeHrxcu8+45uhLet+PzN/xd3fExqU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0GcilQvaIy9OdjDIEPEyKEyyZWfRQKu/yhneB8EpjAUYu8L5AqIRr+565+D/HL/U9KXkCRIEbvDQ/wfMY92S8CTL6DidpWMSL4nvdLaVgqSakcLOO5MjhpKWiSjzxeqwN8A/YeuUQQVQHLDPo+irbGDK6n750ubikSXpk136JXQ=
23, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 22 --  s=s1024; d=yahoo.com;
23, 22 --  h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 22 --  b=sYEzKEy9IG9bV1Nj4rulxBLAZV5AzUoqIyXJc9gRFPrqoywdygd+9jhOGXzeXGGY4m1726+CiEjuVHA+jzfzymba3Mtk3O8aOUGpDSGtjHbv+FwTlUtm9QS3LyaZ0xidvzmjngmb1AmsU2z9yNOM0VLCpsuzsVVIISHYhmwrzHw=;
23, 22 -- Message-ID: {34822.47499.qm-at-web110802.mail.gq1.yahoo.com}
23, 22 -- X-YMail-OSG: CO6_PMQVM1lDrJC.4Vtg74weN1IEJ3v3ITU0Ltgpnyo1QmejxxedyFTptND3afAmRueVCmYuv5EQScekPUy2BRGXkSDN6JgZbmaQxtbJr.TqapZpszOgy6tsOtCvfpw28ZYgw31jTw6j.KJo7_J3iYZANl1tQ0zdMMVOMNTveiEhL0D11G2IVFt4EP3R5A_qSSodAFy4SOiH4jUiX4y1rszQyLFeXfOccrycQbxdhOlhtuczMa7l2hmh6HdlpIfaIqj3WIXveuseMtAauy.Ni0.ZGkJkC1as1bREzhe4nvIN1gn6AF9msON4DBetnkRYdUs_s_OfURyT
23, 22 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Thu, 10 Sep 2009 04:23:25 PDT
23, 22 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.347.2
23, 22 -- Date: Thu, 10 Sep 2009 04:23:25 -0700 (PDT)
23, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 22 -- Subject: Re: [Microscopy] viaWWW: TEM powder sample prep by ion-milling?
23, 22 -- To: microscopy-at-microscopy.com
23, 22 -- MIME-Version: 1.0
23, 22 -- Content-Type: text/plain; charset=iso-8859-1
23, 22 -- Content-Transfer-Encoding: 8bit
23, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8ABNQnl019751
==============================End of - Headers==============================






==============================Original Headers==============================
39, 25 -- From weis183-at-yahoo.fr Thu Sep 10 07:56:32 2009
39, 25 -- Received: from web24603.mail.ird.yahoo.com (web24603.mail.ird.yahoo.com [212.82.104.160])
39, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8ACuVXL004154
39, 25 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 07:56:32 -0500
39, 25 -- Received: (qmail 13321 invoked by uid 60001); 10 Sep 2009 12:56:31 -0000
39, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1252587391; bh=uk92QzIfRui7oOUkVidx2dnUXPWD5Sj+lv2+rOMendU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=HTSOoM12JdrdoX3c+va8E9Kv5bU6zIpRMxmPUWjqpnDmkW7Ltvhg30K/wAwSWRD1RHaIxNXD133AllU7mp2Mv2b94gn+ghveO0bMM0R7ouTrl2/ggk6C0HiIbKwV7nEqFfX9cFaix4Q3RxWVItA7oJUff4YZUlounuA2Q5CfmpU=
39, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
39, 25 -- s=s1024; d=yahoo.fr;
39, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
39, 25 -- b=DEApWYaS6zYAxQv3Lc0XiqHtT5tHIyeBlTSmdfrJdhgH/3WE1ZcflBLPnQPcUG+duVJ3aqBJyyM/134YBEQn9y5o46fHCAd1YR/THfzeHJ7JbUI0ST7OwCm41FgWEnurLuGQAZ0VhQXxLL1v/6bFKBLkHoyrIzhKKmKyr1eW0Vs=;
39, 25 -- Message-ID: {140403.13281.qm-at-web24603.mail.ird.yahoo.com}
39, 25 -- X-YMail-OSG: vLjExIMVM1mG9hEJeMyn0bZbMv6tKI1fFG.6Ho7vTi4wabCy9tHSy6GwSLhzMR7T_rm_oZ7ZW5YJx7qy2zWXeIAd_JHmR1_2ehDL8qnP8BCPjC6aAGoxyi3D2Kv3ZYNYwtBxdu4.v1THW.rGIIUuLpk6nxlymR6RI1QHUol6_h.RctOGGXDR6H__ilb2zxXmFBD61lYhQQIcKWg6XkFpxCuSCzQRW17zXFZAK2JiGT_UyTiRnfq.xfiR079dXoKJ3JD037RPfSCchf28jL3oB0EGZyo2JToirUyJEpFcXug-
39, 25 -- Received: from [147.210.80.23] by web24603.mail.ird.yahoo.com via HTTP; Thu, 10 Sep 2009 12:56:30 GMT
39, 25 -- X-Mailer: YahooMailRC/1358.27 YahooMailWebService/0.7.338.2
39, 25 -- References: {200909101127.n8ABRnMY025510-at-ns.microscopy.com}
39, 25 -- Date: Thu, 10 Sep 2009 12:56:30 +0000 (GMT)
39, 25 -- From: Patrick Weisbecker {weis183-at-yahoo.fr}
39, 25 -- Subject: Re : [Microscopy] Re: viaWWW: TEM powder sample prep by ion-milling?
39, 25 -- To: microscopy-at-microscopy.com
39, 25 -- Cc: z.zhou-at-sheffield.ac.uk
39, 25 -- In-Reply-To: {200909101127.n8ABRnMY025510-at-ns.microscopy.com}
39, 25 -- MIME-Version: 1.0
39, 25 -- Content-Type: text/plain; charset=iso-8859-1
39, 25 -- Content-Transfer-Encoding: 8bit
39, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8ACuVXL004154
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 10 Sep 2009 13:34:05 -0500
Subject: [Microscopy] Weatherforecast: Blue sky, microscopes at 90 feet...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
beforehand I apologize for this not very serious topic.

Today we moved a Jeol 840 to his new home at the Armin-Knab Highschool
at Kitzingen nearby Wuerzburg, Germany.
The sight of the microscope parts up in the air is very special :-)
Feel free to have a look: www.elektronenmikroskopie.info/840_flight

The microscope survived this unusual way and will be up to working
condition tomorrow.

Greetings,
Stefan


--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 20 -- From stefan.diller-at-t-online.de Thu Sep 10 13:34:04 2009
7, 20 -- Received: from mailout09.t-online.de (mailout09.t-online.de [194.25.134.84])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8AIY4bC029100
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 13:34:04 -0500
7, 20 -- Received: from fwd09.aul.t-online.de
7, 20 -- by mailout09.t-online.de with smtp
7, 20 -- id 1MloT5-0007XK-05; Thu, 10 Sep 2009 20:34:03 +0200
7, 20 -- Received: from [192.168.2.101] (G-rmGeZeohojGKK4OMCdUzyhNQNr-B9GwIM-gOPzYRntPhQjAV2Y0Ubp+G5W3XXgjq-at-[93.222.125.20]) by fwd09.aul.t-online.de
7, 20 -- with esmtp id 1MloSz-25LaKm0; Thu, 10 Sep 2009 20:33:57 +0200
7, 20 -- Message-ID: {4AA94695.1060909-at-t-online.de}
7, 20 -- Date: Thu, 10 Sep 2009 20:33:57 +0200
7, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 20 -- MIME-Version: 1.0
7, 20 -- To: microscopy-at-microscopy.com
7, 20 -- Subject: Weatherforecast: Blue sky, microscopes at 90 feet...
7, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- X-ID: G-rmGeZeohojGKK4OMCdUzyhNQNr-B9GwIM-gOPzYRntPhQjAV2Y0Ubp+G5W3XXgjq
7, 20 -- X-TOI-MSGID: 9ee0daac-8619-4db1-9b6e-46944742928b
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 10 Sep 2009 14:37:42 -0500
Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90 feet...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Donald,
sure we normally have SEMs and TEMs at the high-schools in Germany...
(no, I am joking).
I privately set up EM labs at two high-schools with donated instruments
from the industry or from universities. I did the move and the service
and help students with their work with the scopes in my spare time.
And: Yes, this had been my highest move ever of a scope.

Best wishes,
Stefan



Gibbon, Donald L. schrieb:
} You mean to tell us that they have SEMs in high school in Germany now?
} Question: do they fund public education at the local level in Germany or
} is this a private school? I can't imagine the tax payers putting up with
} this kind of high-fallutin' frill in the good ol' US of A! Still the
} whole exercise must have been quite a buzz!
}
} Donald L. Gibbon
}
} -----Original Message-----
} From: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
} Sent: Thursday, September 10, 2009 2:43 PM
} To: Gibbon, Donald L.
} Subject: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
} feet...
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} Dear All,
} beforehand I apologize for this not very serious topic.
}
} Today we moved a Jeol 840 to his new home at the Armin-Knab Highschool
} at Kitzingen nearby Wuerzburg, Germany.
} The sight of the microscope parts up in the air is very special :-)
} Feel free to have a look: www.elektronenmikroskopie.info/840_flight
}
} The microscope survived this unusual way and will be up to working
} condition tomorrow.
}
} Greetings,
} Stefan
}
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Thu Sep 10 14:37:42 2009
7, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8AJbfaC012413
7, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 14:37:41 -0500
7, 22 -- Received: from fwd07.aul.t-online.de
7, 22 -- by mailout02.t-online.de with smtp
7, 22 -- id 1MlpSf-0007fE-01; Thu, 10 Sep 2009 21:37:41 +0200
7, 22 -- Received: from [192.168.2.101] (XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti-at-[93.222.125.20]) by fwd07.aul.t-online.de
7, 22 -- with esmtp id 1MlpSQ-16eS2K0; Thu, 10 Sep 2009 21:37:26 +0200
7, 22 -- Message-ID: {4AA95576.7060509-at-t-online.de}
7, 22 -- Date: Thu, 10 Sep 2009 21:37:26 +0200
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at 90 feet...
7, 22 -- References: {200909101843.n8AIh7Hx009946-at-ns.microscopy.com} {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
7, 22 -- In-Reply-To: {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID: XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti
7, 22 -- X-TOI-MSGID: 7690d2cb-2cde-4483-8b68-d8823bd8708e
==============================End of - Headers==============================




From: shawn_coffee-at-yahoo.com
Date: Thu, 10 Sep 2009 16:48:00 -0500
Subject: [Microscopy] SEM/TEM polymer preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Everyone,


My organization uses a PVD Cr recipe that was developed well over a
decade ago for SEM and TEM preparation. Implementing the recipe with
inorganic samples the recipe produces excellent imaging results.
Recently, our organization has taken on a project that investigates
nanometer scale polymer features utilizing SEM. The Cr recipe deforms
the features slightly. We know the Cr deposition is the causing the
deformation, because similar samples coated with Au/Pd did not deform
the polymer. We know from two random references that using Cr is a poor
organics preparation technique. Several questions arise from our
dilemma.


1. Why is polymer sample preparation using PVD Cr causing deformation?


2. Is it possible to reduce or eliminate polymer deformation from the
Cr coating by adjusting PVD conditions (power, pressure)?


3. What is the best metal/material to coat polymers and a good, corresponding
PVD recipe?


Thanks for your help,

Shawn Coffee, Ph D


Cerium Laboratories, LLC

http://www.ceriumlabs.com/

Office Phone: 512-934-5256

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
17, 20 -- From shawn_coffee-at-yahoo.com Thu Sep 10 16:48:00 2009
17, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138])
17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8ALm073029937
17, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 16:48:00 -0500
17, 20 -- Received: (qmail 9806 invoked by uid 60001); 10 Sep 2009 21:47:59 -0000
17, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1252619279; bh=PirF4CZoWBPwPUoVOGhgOiYUT9pH0xNehu/7uBRMhO4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=eKMHBx/Rta7FCZb39EFbh1GgpRSyvNwmgiQ+Phmz4RCpJweS0/3XfSsvvZ3m6O0H2avco0Gh81qKuipgtQqoyE4MAz71kroUlG+1Z4joXciqrZbtNH0wLxDTG9Etxwz1RcawZbMtwHG2dFHVn0m2mVHIJQ8myXLbdcMcJF52/9Y=
17, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
17, 20 -- s=s1024; d=yahoo.com;
17, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
17, 20 -- b=d8s5ka0ffuH6UH5TroycX/4WGnl3XR4jq5qBjvsjGlS1ZyAcbtQcy6+PedG5RaM0md7PoDHPP9Zf1VTdo9+fN2JIv/kCC22QhwGUiuAAx5wOIhNBSj0jjAvosXETc+0l8jWGjFrGRNHt43rSJhZpNRAwTWWO0QQaDk7hbcNabmY=;
17, 20 -- Message-ID: {743422.9621.qm-at-web37406.mail.mud.yahoo.com}
17, 20 -- X-YMail-OSG: VR3g2MEVM1l.Y89f4YKtYqGfw9vTWjfxk9rCgmTlI.Ud3t9mlInPyEHbzqontUIYNyt_MXsCplMS6h0Cmd9i0XP6EXl0yT48saJK9fuAb7CQ3Z9G1_fdLpINdEOqFes9m.GxF_nRMXzvlDPTBrUJBTX7DQWVC0H0yxvGQ3Vq8TNxW1nx9oZMf74e5eBg_0oZX9ihYavHjNN27QD.mF_dz.Rgyy1VpFonI0lZfWcPZOXLzVh0yIVu1k2qjAMaURKJK7YKjlyhYtEg89hzicB0HBX2I3A4FwSbL6_FIAsR4zTQPhp30S3x_q4nd3IeK3dNcfU7UR3Bh6U.DOuu.WCCn9AJ7VvmeYYC7AjZti1r_KQLsVleCZMzsuXn23ssjvY0oA.8WX7pQYTQec6UAj34PjNa
17, 20 -- Received: from [12.110.209.193] by web37406.mail.mud.yahoo.com via HTTP; Thu, 10 Sep 2009 14:47:59 PDT
17, 20 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.338.2
17, 20 -- Date: Thu, 10 Sep 2009 14:47:59 -0700 (PDT)
17, 20 -- From: Shawn Coffee {shawn_coffee-at-yahoo.com}
17, 20 -- Subject: SEM/TEM polymer preparation
17, 20 -- To: Microscopy-at-microscopy.com
17, 20 -- MIME-Version: 1.0
17, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: neerajg-at-clemson.edu
Date: Thu, 10 Sep 2009 18:38:37 -0500
Subject: [Microscopy] viaWWW: Paraplast X-tra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both neerajg-at-clemson.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: neerajg-at-clemson.edu
Name: Neeraj

Organization: Clemson University

Title-Subject: [Filtered] Paraplast X-tra

Question: Deal List,

Our lab has a substantial stockpile of Paraplast X-tra paraffin based
embedding medium for histology. Our stockpile is rather old, about
5-6 years, do these media have a shelf life after which you start
seeing adverse effects on embedding?

Best Regards,

Neeraj.



Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Please note my new email address: neerajg-at-clemson.edu





Login Host: 130.127.121.55
---------------------------------------------------------------------------

==============================Original Headers==============================
17, 11 -- From zaluzec-at-microscopy.com Thu Sep 10 18:38:37 2009
17, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ANcak1015068
17, 11 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 18:38:36 -0500
17, 11 -- Mime-Version: 1.0
17, 11 -- Message-Id: {p06240800c6cf3e6ad67b-at-[206.69.208.22]}
17, 11 -- Date: Thu, 10 Sep 2009 18:38:35 -0500
17, 11 -- To: microscopy-at-microscopy.com
17, 11 -- From: neerajg-at-clemson.edu (by way of MicroscopyListserver)
17, 11 -- Subject: viaWWW: Paraplast X-tra
17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 11 Sep 2009 09:27:23 -0500
Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don,
You're right. Even getting the space for a donated SEM with donated service
can be a real trauma, but some high schools around the country (including
Red Lion, PA) are forward thinking enough to make the accommodation.

If we, as a country, are truly concerned with the lack of interest in
science, engineering and math, then maybe we should be making a greater
effort to let kids play with the toys, er, use the tools, that real
scientists and other professionals use in the real world. I've actually had
many a person in the sciences tell me that a major reason they went into
whatever science they went into is because of all the neat toys they get to
use.

Free SEMS and TEMs are not that difficult to come by. The problem is the
cost of service. I would encourage anyone in field service to take on one
pro bono instrument for a high school. I would also encourage the
manufacturers to support this idea. Support from local businesses is also
very much needed for operating supplies and sometimes for essential
equipment that is missing during set-up, such air compressors or coaters.

Having supported the Red Lion, PA SEM for the past 9 years, I'm now in an
uphill effort to get the space in a very financially strapped high school in
Bridgton, ME. It may take a while. Red Lion didn't take the microscope
until 7 years after I first made the offer. Sometimes a change in personnel
is needed.

As to "I can't imagine the tax payers putting up with this kind of
high-fallutin' frill in the good ol' US of A!", my hope is that someday the
American taxpayer is going to recognize that you get what you pay for, and
that there is no free lunch. You can decide to buy quality and pay as you
go (tax and spend), buy quality and charge it to your grandchildren (borrow
and spend), or buy cheap and let your grandchildren pay for the
infrastructure upgrades (I've got mine. Who cares about you?)

End of rant.

Please consider helping more high schools acquire decent equipment to
encourage students to go into technical fields.

Ken Converse,
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Thursday, September 10, 2009 3:40 PM
To: kenconverse-at-qualityimages.biz

Dear Donald,
sure we normally have SEMs and TEMs at the high-schools in Germany...
(no, I am joking).
I privately set up EM labs at two high-schools with donated instruments
from the industry or from universities. I did the move and the service
and help students with their work with the scopes in my spare time.
And: Yes, this had been my highest move ever of a scope.

Best wishes,
Stefan



Gibbon, Donald L. schrieb:
} You mean to tell us that they have SEMs in high school in Germany now?
} Question: do they fund public education at the local level in Germany or
} is this a private school? I can't imagine the tax payers putting up with
} this kind of high-fallutin' frill in the good ol' US of A! Still the
} whole exercise must have been quite a buzz!
}
} Donald L. Gibbon
}
} -----Original Message-----
} From: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
} Sent: Thursday, September 10, 2009 2:43 PM
} To: Gibbon, Donald L.
} Subject: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
} feet...
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} ----
}
} Dear All,
} beforehand I apologize for this not very serious topic.
}
} Today we moved a Jeol 840 to his new home at the Armin-Knab Highschool
} at Kitzingen nearby Wuerzburg, Germany.
} The sight of the microscope parts up in the air is very special :-)
} Feel free to have a look: www.elektronenmikroskopie.info/840_flight
}
} The microscope survived this unusual way and will be up to working
} condition tomorrow.
}
} Greetings,
} Stefan
}
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Thu Sep 10 14:37:42 2009
7, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de
[194.25.134.17])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n8AJbfaC012413
7, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 14:37:41
-0500
7, 22 -- Received: from fwd07.aul.t-online.de
7, 22 -- by mailout02.t-online.de with smtp
7, 22 -- id 1MlpSf-0007fE-01; Thu, 10 Sep 2009 21:37:41 +0200
7, 22 -- Received: from [192.168.2.101]
(XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti-at-[93.222.
125.20]) by fwd07.aul.t-online.de
7, 22 -- with esmtp id 1MlpSQ-16eS2K0; Thu, 10 Sep 2009 21:37:26
+0200
7, 22 -- Message-ID: {4AA95576.7060509-at-t-online.de}
7, 22 -- Date: Thu, 10 Sep 2009 21:37:26 +0200
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at
90 feet...
7, 22 -- References: {200909101843.n8AIh7Hx009946-at-ns.microscopy.com}
{AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
7, 22 -- In-Reply-To:
{AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID:
XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti
7, 22 -- X-TOI-MSGID: 7690d2cb-2cde-4483-8b68-d8823bd8708e
==============================End of - Headers==============================




==============================Original Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Fri Sep 11 09:27:23 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BERMUT007289
25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 09:27:22 -0500
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20090911142720799.JYWB26991-at-cdptpa-omta03.mail.rr.com}
25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 14:27:20 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90 feet...
25, 25 -- Date: Fri, 11 Sep 2009 10:27:10 -0400
25, 25 -- Message-ID: {CFF3847978814AF292242D5CDECA4DAA-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="us-ascii"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- Importance: Normal
25, 25 -- Thread-Index: AcoyTnEcmKszbC1YTdWweTONXwmR3QAmG04g
25, 25 -- In-Reply-To: {200909101939.n8AJdfrr016203-at-ns.microscopy.com}
25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8BERMUT007289
==============================End of - Headers==============================




From: joelsheffield-at-gmail.com
Date: Fri, 11 Sep 2009 10:11:38 -0500
Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90 feet...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It seems to me that FEI had one of their table-top SEMs installed at a local Philadelphia High
School. These require much less care and feeding than older models. At the same time, I
applaud Ken's comment about introduction of modern science into high school curricula --and not
just as knowledge, but as hands-on experience.

Joel





On 11 Sep 2009 at 9:36, kenconverse-at-qualityimages.biz wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Don,
} You're right. Even getting the space for a donated SEM with donated service
} can be a real trauma, but some high schools around the country (including
} Red Lion, PA) are forward thinking enough to make the accommodation.
}
} If we, as a country, are truly concerned with the lack of interest in
} science, engineering and math, then maybe we should be making a greater
} effort to let kids play with the toys, er, use the tools, that real
} scientists and other professionals use in the real world. I've actually had
} many a person in the sciences tell me that a major reason they went into
} whatever science they went into is because of all the neat toys they get to
} use.
}
} Free SEMS and TEMs are not that difficult to come by. The problem is the
} cost of service. I would encourage anyone in field service to take on one
} pro bono instrument for a high school. I would also encourage the
} manufacturers to support this idea. Support from local businesses is also
} very much needed for operating supplies and sometimes for essential
} equipment that is missing during set-up, such air compressors or coaters.
}
} Having supported the Red Lion, PA SEM for the past 9 years, I'm now in an
} uphill effort to get the space in a very financially strapped high school in
} Bridgton, ME. It may take a while. Red Lion didn't take the microscope
} until 7 years after I first made the offer. Sometimes a change in personnel
} is needed.
}
} As to "I can't imagine the tax payers putting up with this kind of
} high-fallutin' frill in the good ol' US of A!", my hope is that someday the
} American taxpayer is going to recognize that you get what you pay for, and
} that there is no free lunch. You can decide to buy quality and pay as you
} go (tax and spend), buy quality and charge it to your grandchildren (borrow
} and spend), or buy cheap and let your grandchildren pay for the
} infrastructure upgrades (I've got mine. Who cares about you?)
}
} End of rant.
}
} Please consider helping more high schools acquire decent equipment to
} encourage students to go into technical fields.
}
} Ken Converse,
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
} Sent: Thursday, September 10, 2009 3:40 PM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90
} feet...
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Donald,
} sure we normally have SEMs and TEMs at the high-schools in Germany...
} (no, I am joking).
} I privately set up EM labs at two high-schools with donated instruments
} from the industry or from universities. I did the move and the service
} and help students with their work with the scopes in my spare time.
} And: Yes, this had been my highest move ever of a scope.
}
} Best wishes,
} Stefan
}
}
}
} Gibbon, Donald L. schrieb:
} } You mean to tell us that they have SEMs in high school in Germany now?
} } Question: do they fund public education at the local level in Germany or
} } is this a private school? I can't imagine the tax payers putting up with
} } this kind of high-fallutin' frill in the good ol' US of A! Still the
} } whole exercise must have been quite a buzz!
} }
} } Donald L. Gibbon
} }
} } -----Original Message-----
} } From: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
} } Sent: Thursday, September 10, 2009 2:43 PM
} } To: Gibbon, Donald L.
} } Subject: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
} } feet...
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } ----
} }
} } Dear All,
} } beforehand I apologize for this not very serious topic.
} }
} } Today we moved a Jeol 840 to his new home at the Armin-Knab Highschool
} } at Kitzingen nearby Wuerzburg, Germany.
} } The sight of the microscope parts up in the air is very special :-)
} } Feel free to have a look: www.elektronenmikroskopie.info/840_flight
} }
} } The microscope survived this unusual way and will be up to working
} } condition tomorrow.
} }
} } Greetings,
} } Stefan
} }
} }
}
} --
} -----------------------------------------------------
} Stefan Diller - Wissenschaftliche Photographie
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49-931-7848700 Phone
} ++49-931-7848701 Fax
} ++49-175-7177051 Mobile
}
} Websites:
} www.stefan-diller.com
} www.elektronenmikroskopie.info
} www.assisi.de
} www.zwillingsprojekt.de
} Anfahrt: http://Mail.map24.com/Stefan.Diller
} -----------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 22 -- From stefan.diller-at-t-online.de Thu Sep 10 14:37:42 2009
} 7, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de
} [194.25.134.17])
} 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n8AJbfaC012413
} 7, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 14:37:41
} -0500
} 7, 22 -- Received: from fwd07.aul.t-online.de
} 7, 22 -- by mailout02.t-online.de with smtp
} 7, 22 -- id 1MlpSf-0007fE-01; Thu, 10 Sep 2009 21:37:41 +0200
} 7, 22 -- Received: from [192.168.2.101]
} (XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti-at-[93.222.
} 125.20]) by fwd07.aul.t-online.de
} 7, 22 -- with esmtp id 1MlpSQ-16eS2K0; Thu, 10 Sep 2009 21:37:26
} +0200
} 7, 22 -- Message-ID: {4AA95576.7060509-at-t-online.de}
} 7, 22 -- Date: Thu, 10 Sep 2009 21:37:26 +0200
} 7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
} 7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
} 7, 22 -- MIME-Version: 1.0
} 7, 22 -- To: microscopy-at-microscopy.com
} 7, 22 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at
} 90 feet...
} 7, 22 -- References: {200909101843.n8AIh7Hx009946-at-ns.microscopy.com}
} {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
} 7, 22 -- In-Reply-To:
} {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 22 -- Content-Transfer-Encoding: 7bit
} 7, 22 -- X-ID:
} XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti
} 7, 22 -- X-TOI-MSGID: 7690d2cb-2cde-4483-8b68-d8823bd8708e
} ==============================End of - Headers==============================
}
}
}
}
} ==============================Original Headers==============================
} 25, 25 -- From kenconverse-at-qualityimages.biz Fri Sep 11 09:27:23 2009
} 25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120])
} 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BERMUT007289
} 25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 09:27:22 -0500
} 25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
} 25, 25 -- with ESMTP
} 25, 25 -- id {20090911142720799.JYWB26991-at-cdptpa-omta03.mail.rr.com}
} 25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 14:27:20 +0000
} 25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} 25, 25 -- To: "MSA Listserver" {microscopy-at-microscopy.com}
} 25, 25 -- Subject: RE: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90 feet...
} 25, 25 -- Date: Fri, 11 Sep 2009 10:27:10 -0400
} 25, 25 -- Message-ID: {CFF3847978814AF292242D5CDECA4DAA-at-Ken}
} 25, 25 -- MIME-Version: 1.0
} 25, 25 -- Content-Type: text/plain;
} 25, 25 -- charset="us-ascii"
} 25, 25 -- X-Priority: 3 (Normal)
} 25, 25 -- X-MSMail-Priority: Normal
} 25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
} 25, 25 -- Importance: Normal
} 25, 25 -- Thread-Index: AcoyTnEcmKszbC1YTdWweTONXwmR3QAmG04g
} 25, 25 -- In-Reply-To: {200909101939.n8AJdfrr016203-at-ns.microscopy.com}
} 25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
} 25, 25 -- Content-Transfer-Encoding: 8bit
} 25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8BERMUT007289
} ==============================End of - Headers==============================


--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
11, 42 -- From joelsheffield-at-gmail.com Fri Sep 11 10:11:38 2009
11, 42 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.247])
11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BFBcnW022889
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 10:11:38 -0500
11, 42 -- Received: by an-out-0708.google.com with SMTP id c38so363425ana.0
11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 08:11:38 -0700 (PDT)
11, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 42 -- d=gmail.com; s=gamma;
11, 42 -- h=domainkey-signature:received:received:from:to:date:mime-version
11, 42 -- :subject:message-id:priority:in-reply-to:references:x-mailer
11, 42 -- :content-type:content-transfer-encoding:content-description;
11, 42 -- bh=70qqvce8w5A3i9IgP4914cvkCqyMN24T1NbHHxWao2k=;
11, 42 -- b=ZvPc7u/Vj7XOEbUMZccs3r6RliI45dNbkKdb77JZPYyiDWllY8QKKFGrl6RV/qgwls
11, 42 -- 6eZeUFYWR+I11WvAaWvqBLfONjoeAKf1Rmw6UnU1ulBpNtlBpGUwtM7TrCHr6kywcwS6
11, 42 -- sPqN2FpfWwYOnT9rfAg+QPLhnDOeadLW9zxPk=
11, 42 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
11, 42 -- d=gmail.com; s=gamma;
11, 42 -- h=from:to:date:mime-version:subject:message-id:priority:in-reply-to
11, 42 -- :references:x-mailer:content-type:content-transfer-encoding
11, 42 -- :content-description;
11, 42 -- b=cFmj+zpI5R9YUJBnczaPa7PJgDxTh2//zMmcx/trjUf4LkhytGaBL7DsegDqVEgqZx
11, 42 -- LqX1dbRs+ST7u1CIS/pHTmhc9TqQ0Po7N47zkPnhyGXMxzNXjWuNNcOa6M58i3rIKO0+
11, 42 -- dVHt1ZpyKf1sfYEbn332IGe+mTdvBjkh/CHCI=
11, 42 -- Received: by 10.101.26.26 with SMTP id d26mr3373968anj.9.1252681897606;
11, 42 -- Fri, 11 Sep 2009 08:11:37 -0700 (PDT)
11, 42 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40])
11, 42 -- by mx.google.com with ESMTPS id 39sm3206226aga.31.2009.09.11.08.11.35
11, 42 -- (version=SSLv3 cipher=RC4-MD5);
11, 42 -- Fri, 11 Sep 2009 08:11:36 -0700 (PDT)
11, 42 -- From: joelsheffield-at-gmail.com
11, 42 -- To: kenconverse-at-qualityimages.biz, microscopy-at-microscopy.com
11, 42 -- Date: Fri, 11 Sep 2009 11:11:34 -0400
11, 42 -- MIME-Version: 1.0
11, 42 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at 90 feet...
11, 42 -- Message-ID: {4AAA3066.3005.6DC4038-at-joelsheffield.gmail.com}
11, 42 -- Priority: normal
11, 42 -- In-reply-to: {200909111436.n8BEaemX019713-at-ns.microscopy.com}
11, 42 -- References: {200909111436.n8BEaemX019713-at-ns.microscopy.com}
11, 42 -- X-mailer: Pegasus Mail for Windows (4.41)
11, 42 -- Content-type: text/plain; charset=US-ASCII
11, 42 -- Content-transfer-encoding: 7BIT
11, 42 -- Content-description: Mail message body
==============================End of - Headers==============================




From: schooley-at-mcn.org
Date: Fri, 11 Sep 2009 12:30:45 -0500
Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I realized this morming that I sent this only to Stefan, I meant to send
it to the whole group... I also agree with Ken...

dj

---------- Forwarded message ----------

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Don & all-

This conversation about EMs in high schools is very encouraging, but
there's another point that needs to be made. The determined
volunteers who are actually doing this are mostly working in
isolation. Project MICRO is MSA's middle school outreach, but if all
microscopists who are working with a high school will please respond
to me, I'll make a list. And then I'll give that list to a VERY
dedicated person who can set up & maintain a contact network for
MSA's Education Committee. Who wants to be that volunteer?

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
45301 Caspar Point Road, Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
4, 18 -- From schooley-at-mcn.org Fri Sep 11 12:30:45 2009
4, 18 -- Received: from pop1.mcn.org (pop1.mcn.org [216.150.240.64])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BHUiZd027174
4, 18 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 12:30:45 -0500
4, 18 -- Received: from [66.81.37.248] (helo=[10.0.1.2])
4, 18 -- by pop1.mcn.org with esmtpa (Exim 4.69)
4, 18 -- (envelope-from {schooley-at-mcn.org} )
4, 18 -- id 1Mm9xF-0001a6-3k; Fri, 11 Sep 2009 10:30:38 -0700
4, 18 -- Mime-Version: 1.0
4, 18 -- Message-Id: {a06200700c6d032ddfc6d-at-[10.0.1.2]}
4, 18 -- In-Reply-To: {200909111608.n8BG89op019704-at-ns.microscopy.com}
4, 18 -- References: {200909111608.n8BG89op019704-at-ns.microscopy.com}
4, 18 -- Date: Fri, 11 Sep 2009 10:28:24 -0700
4, 18 -- To: dljones-at-bestweb.net, microscopy-at-microscopy.com
4, 18 -- From: Caroline Schooley {schooley-at-mcn.org}
4, 18 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
4, 18 -- Cc: morales87-at-yahoo.com, lga-at-lawrencegaltman.com, john_mackenzie-at-ncsu.edu
4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Hobie-at-technicalsalessolutions.com
Date: Fri, 11 Sep 2009 13:14:01 -0500
Subject: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI, our company donated a digital SEM to a local HS in Portland, OR a
little over a year ago.

The SEM program is working with great success at the high school level, and
now they have expanded use of the SEM to their 7th-8th graders.

Keep it up all!


On 9/11/09 9:08 AM, "dljones-at-bestweb.net" {dljones-at-bestweb.net} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I realized this morming that I sent this only to Stefan, I meant to send
} it to the whole group... I also agree with Ken...
}
} dj
}
} ---------- Forwarded message ----------
} Date: Thu, 10 Sep 2009 20:17:41 +0000 (UTC)
} X-from: dljones {dljones-at-bestweb.net}
} To: stefan.diller-at-t-online.de
} Subject: Re: [Microscopy] Re: Weatherforecast: Blue sky, microscopes at 90
} feet...
}
} Donald,
}
} I have also set up an SEM in a high school here in the USA and know of
} numerous high schools here that have them. I'm also working right now on
} setting up another SEM for use by more than one local high school.
}
} The instrument that is currently set up is also now being used in a joint
} agreement with a local community college where the college can access it
} for their students, and the students coming out of the high school that
} take the advanced science courses now being offered are given college
} credits useable within the state colleges here. So graduating seniors
} can get, I believe up to 8 college credits, if they've taken all the
} advanced classes offered... darned good I think...
}
} We do need to have our high schools getting up to speed in our ever
} increasingly competitive world.... just my personal opinion...
}
} Stefan - great job!
}
} dj
}
} On Thu, 10 Sep 2009, stefan.diller-at-t-online.de wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear Donald,
} } sure we normally have SEMs and TEMs at the high-schools in Germany...
} } (no, I am joking).
} } I privately set up EM labs at two high-schools with donated instruments
} } from the industry or from universities. I did the move and the service
} } and help students with their work with the scopes in my spare time.
} } And: Yes, this had been my highest move ever of a scope.
} }
} } Best wishes,
} } Stefan
} }
} }
} }
} } Gibbon, Donald L. schrieb:
} } } You mean to tell us that they have SEMs in high school in Germany now?
} } } Question: do they fund public education at the local level in Germany or
} } } is this a private school? I can't imagine the tax payers putting up with
} } } this kind of high-fallutin' frill in the good ol' US of A! Still the
} } } whole exercise must have been quite a buzz!
} } }
} } } Donald L. Gibbon
} } }
} } } -----Original Message-----
} } } From: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
} } } Sent: Thursday, September 10, 2009 2:43 PM
} } } To: Gibbon, Donald L.
} } } Subject: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
} } } feet...
} } }
} } }
} } }
} } }
} } } ------------------------------------------------------------------------
} } } ----
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ------------------------------------------------------------------------
} } } ----
} } }
} } } Dear All,
} } } beforehand I apologize for this not very serious topic.
} } }
} } } Today we moved a Jeol 840 to his new home at the Armin-Knab Highschool
} } } at Kitzingen nearby Wuerzburg, Germany.
} } } The sight of the microscope parts up in the air is very special :-)
} } } Feel free to have a look: www.elektronenmikroskopie.info/840_flight
} } }
} } } The microscope survived this unusual way and will be up to working
} } } condition tomorrow.
} } }
} } } Greetings,
} } } Stefan
} } }
} } }
} }
} } --
} } -----------------------------------------------------
} } Stefan Diller - Wissenschaftliche Photographie
} } Arndtstrasse 22
} } D - 97072 Wuerzburg Germany
} } ++49-931-7848700 Phone
} } ++49-931-7848701 Fax
} } ++49-175-7177051 Mobile
} }
} } Websites:
} } www.stefan-diller.com
} } www.elektronenmikroskopie.info
} } www.assisi.de
} } www.zwillingsprojekt.de
} } Anfahrt: http://Mail.map24.com/Stefan.Diller
} } -----------------------------------------------------
} }
} } ==============================Original Headers==============================
} } 7, 22 -- From stefan.diller-at-t-online.de Thu Sep 10 14:37:42 2009
} } 7, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de
} } [194.25.134.17])
} } 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n8AJbfaC012413
} } 7, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2009 14:37:41 -0500
} } 7, 22 -- Received: from fwd07.aul.t-online.de
} } 7, 22 -- by mailout02.t-online.de with smtp
} } 7, 22 -- id 1MlpSf-0007fE-01; Thu, 10 Sep 2009 21:37:41 +0200
} } 7, 22 -- Received: from [192.168.2.101]
} } (XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti-at-[93.222.1
} } 25.20]) by fwd07.aul.t-online.de
} } 7, 22 -- with esmtp id 1MlpSQ-16eS2K0; Thu, 10 Sep 2009 21:37:26 +0200
} } 7, 22 -- Message-ID: {4AA95576.7060509-at-t-online.de}
} } 7, 22 -- Date: Thu, 10 Sep 2009 21:37:26 +0200
} } 7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
} } 7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
} } 7, 22 -- MIME-Version: 1.0
} } 7, 22 -- To: microscopy-at-microscopy.com
} } 7, 22 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at
} } 90 feet...
} } 7, 22 -- References: {200909101843.n8AIh7Hx009946-at-ns.microscopy.com}
} } {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
} } 7, 22 -- In-Reply-To:
} } {AE9049B5C6BD064BA3F0B94A1F985BA7DE5E14-at-valmail.na.valmont.com}
} } 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 7, 22 -- Content-Transfer-Encoding: 7bit
} } 7, 22 -- X-ID:
} } XpKBuoZaZhG82I5+2ZSl1VhzDbIgvxPA0ksNMOh7G9d3RAKG355qCe5+gr0GCFnQti
} } 7, 22 -- X-TOI-MSGID: 7690d2cb-2cde-4483-8b68-d8823bd8708e
} } ==============================End of - Headers==============================
} }
}
} ==============================Original Headers==============================
} 11, 17 -- From dljones-at-bestweb.net Fri Sep 11 11:01:51 2009
} 11, 17 -- Received: from smtp2.bestweb.net (smtp2.bestweb.net [209.94.103.42])
} 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n8BG1p0q008434
} 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 11:01:51 -0500
} 11, 17 -- Received: from monet.bestweb.net (monet.bestweb.net
} [209.94.121.202])
} 11, 17 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
} 11, 17 -- (No client certificate requested)
} 11, 17 -- by smtp2.bestweb.net (Postfix) with ESMTPS id A2700119DA5
} 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 12:01:50 -0400
} (EDT)
} 11, 17 -- Date: Fri, 11 Sep 2009 15:38:45 +0000 (UTC)
} 11, 17 -- From: dljones {dljones-at-bestweb.net}
} 11, 17 -- To: microscopy-at-microscopy.com
} 11, 17 -- Subject: Re: [Microscopy] Re: Weatherforecast: Blue sky, microscopes
} at 90
} 11, 17 -- feet... (fwd)
} 11, 17 -- Message-ID: {Pine.BSF.4.64.0909111537090.65861-at-monet.bestweb.net}
} 11, 17 -- MIME-Version: 1.0
} 11, 17 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} ==============================End of - Headers==============================
}



==============================Original Headers==============================
8, 20 -- From Hobie-at-technicalsalessolutions.com Fri Sep 11 13:14:00 2009
8, 20 -- Received: from host203.com (host203.com [203.194.159.243])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BIDxQ4009984
8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 13:14:00 -0500
8, 20 -- Received: (qmail 13912 invoked by uid 503); 11 Sep 2009 18:13:56 -0000
8, 20 -- Received: from 71-20-188-20.war.clearwire-wmx.net (HELO ?10.0.1.30?) (Hobie-at-71.20.188.20)
8, 20 -- by host203.com with ESMTPA; 11 Sep 2009 18:13:56 -0000
8, 20 -- User-Agent: Microsoft-Entourage/12.0.0.071130
8, 20 -- Date: Fri, 11 Sep 2009 11:13:52 -0700
8, 20 -- Subject: Re: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
8, 20 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com}
8, 20 -- To: {microscopy-at-microscopy.com}
8, 20 -- Message-ID: {C6CFE170.1B9FA%Hobie-at-technicalsalessolutions.com}
8, 20 -- Thread-Topic: [Microscopy] Weatherforecast: Blue sky, microscopes at 90
8, 20 -- Thread-Index: AcozC53ihZisym306k67B52B6wpP0Q==
8, 20 -- In-Reply-To: {200909111608.n8BG80qM019423-at-ns.microscopy.com}
8, 20 -- Mime-version: 1.0
8, 20 -- Content-type: text/plain;
8, 20 -- charset="US-ASCII"
8, 20 -- Content-transfer-encoding: 7bit
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Fri, 11 Sep 2009 14:02:54 -0500
Subject: [Microscopy] SEM preparation of egg-white

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
anybody out there who can give me a hint how to fix and visualize
egg-white in the SEM?
The image I saw shows a cristaline lamella structure looking like
salicylic acid...

Best wishes,
Stefan


--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
5, 20 -- From stefan.diller-at-t-online.de Fri Sep 11 14:02:54 2009
5, 20 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8BJ2rm4025568
5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 11 Sep 2009 14:02:54 -0500
5, 20 -- Received: from fwd04.aul.t-online.de
5, 20 -- by mailout04.t-online.de with smtp
5, 20 -- id 1MmBOW-0005KA-03; Fri, 11 Sep 2009 21:02:52 +0200
5, 20 -- Received: from [192.168.2.101] (Z4DuMEZLghePFIG4wB-Za3wgSZ+blyo4xudmr1RgBFAMBkdvTsPbE0iBY9jUDbLgg4-at-[93.222.97.163]) by fwd04.aul.t-online.de
5, 20 -- with esmtp id 1MmBON-0GX8KG0; Fri, 11 Sep 2009 21:02:43 +0200
5, 20 -- Message-ID: {4AAA9ED3.7040700-at-t-online.de}
5, 20 -- Date: Fri, 11 Sep 2009 21:02:43 +0200
5, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
5, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
5, 20 -- MIME-Version: 1.0
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- Subject: SEM preparation of egg-white
5, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
5, 20 -- Content-Transfer-Encoding: 7bit
5, 20 -- X-ID: Z4DuMEZLghePFIG4wB-Za3wgSZ+blyo4xudmr1RgBFAMBkdvTsPbE0iBY9jUDbLgg4
5, 20 -- X-TOI-MSGID: 2a7f2879-12dc-4bc1-8a9b-ce1004785789
==============================End of - Headers==============================




From: rick_van-at-aad.gov.au
Date: Mon, 14 Sep 2009 08:03:25 -0500
Subject: [Microscopy] viaWWW: Osmium Plasma Coater versus high res sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Sorry for any delay but I am in South Africa at the moment and have been
unable to collect my emails till now.

Yes, you should under normal conditions set up at the eucentric point. This
action will have set the physical focal length to that required by the
instrument design. This, as I have mentioned, determines, resolution,
contrast and magnification level. For many years I have used TEM outside
these parameters. If I wanted a higher resolution than the manufacturer
offered I would lower the stage (focus clockwise) increasing the objective
lens strength, the magnification is increased and the resolution.
Conversely, with low contrast levels from a biological sample I have raised
the stage as increasing the specimen to objective aperture distance results
in an increase in contrast,

You will need a magnification calibration sample in order to calibrate the
instrument over the magnification range that you require. You will almost
certainly find that there will be a simple % change related to the lower
level of objective current than normal; but I am unable to offer a formula
for you.

Good luck.

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com

----- Original Message -----
X-from: "Ayten Celik-Aktas" {celikaktas-at-gmail.com}
To: {protrain-at-emcourses.com}
Sent: Monday, September 07, 2009 8:12 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rick_van-at-aad.gov.au as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rick_van-at-aad.gov.au
Name: Rick van den Enden

Organization: Australian Antarctic Division

Title-Subject: [Filtered] Osmium Plasma Coater versus high res sputter coater

Question: Dear Listeners,

I am about to update my sputter coater and interested in feedback
from people who may have gone through this process recently. I have
been considering the Cressington 208HR and the Emitech K575X and
wondering if the Filgen Osmium Plasma Coater is a better option for
high resolution coating. I am interested to hear from anyone who has
experience with these instrument? My application is mostly with
biolocal material, particularly phytoplanton using a FESEM.

Thank you and kind regards,

Rick

Login Host: 147.66.8.158
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Sep 14 08:03:25 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ED3OYK014406
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Sep 2009 08:03:24 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c6d3ef80c03b-at-[206.69.208.22]}
9, 11 -- Date: Mon, 14 Sep 2009 08:03:24 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: rick_van-at-aad.gov.au (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Osmium Plasma Coater versus high res sputter coater
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: corvos-at-aol.com
Date: Tue, 15 Sep 2009 07:30:13 -0500
Subject: [Microscopy] viaWWW: Siemens KPY 42-MA pressure sensor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both corvos-at-aol.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: corvos-at-aol.com
Name: Walter Protheroe

Organization: E-MAC, Inc

Title-Subject: [Filtered] Siemens KPY 42-MA pressure sensor

Question: I have a customer who is looking for the following part:
Siemens KPY 42-MA pressure sensor

If anyone has this part or knows where to get it, please contact:

REX Rideout at the Colorado School of Mines
rrideout-at-mines.edu

Thank you,

Walter Protheroe
E-MAC, Inc.

Login Host: 205.188.116.67
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Sep 15 07:30:12 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8FCUBED009962
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2009 07:30:12 -0500
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c6d539410b06-at-[206.69.208.22]}
10, 11 -- Date: Tue, 15 Sep 2009 07:30:11 -0500
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: corvos-at-aol.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Siemens KPY 42-MA pressure sensor
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mdyousuf-at-qu.edu.qa
Date: Tue, 15 Sep 2009 07:30:47 -0500
Subject: [Microscopy] viaWWW: AFM selection and purchase suggestions - please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mdyousuf-at-qu.edu.qa as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mdyousuf-at-qu.edu.qa
Name: Mohammed Yousuf Abdul-Rawoof

Organization: Qatar University

Title-Subject: [Filtered] AFM selection and purchase suggestions - please

Question: Dear Listers,
This is to seek your valuable suggestions regards to the purchase of
an AFM for possible use in a multi-purpose setting (material science
and bio-materials). I look forwards to some sound advice from
experienced users of these instruments and suggest manufacterers and
models. Since, this would a first instrument of its kind to be added
to our laboratory; I wonder what sort of specifications we should opt?
Suggestions on price/performance might be of help. Please respond
off-list or share your thoughts online if it helps the list community
in general.
Thanks

Mohammed Yousuf Abdul-Rawoof
Research Fellow
Electron Microscopy Section
Central Laboratories Unit
Qatar University
POB 2713
Doha, Qatar.
+974-5720907


Login Host: 86.36.66.129
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Sep 15 07:30:46 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8FCUjDe010533
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2009 07:30:46 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240802c6d5395910a5-at-[206.69.208.22]}
8, 11 -- Date: Tue, 15 Sep 2009 07:30:44 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mdyousuf-at-qu.edu.qa (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: AFM selection and purchase suggestions - please
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Henning.Stahlberg-at-unibas.ch
Date: Wed, 16 Sep 2009 09:13:11 -0500
Subject: [Microscopy] viaWWW: EM Service Engineer position in Basel, Switzerland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Henning.Stahlberg-at-unibas.ch as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Henning.Stahlberg-at-unibas.ch
Name: Henning Stahlberg

Organization: University Basel

Title-Subject: [Filtered] EM Service Engineer position in Basel, Switzerland

Question:
A position for an EM Service Engineer is available in the Center for
Cellular Imaging and Nano Analytics (C-CINA) at the University Basel,
Switzerland.


Equipment in C-CINA includes an FEI Titan Krios/GIF, a Philips
CM200FEG, a T12, a CM10, a VG HB-5, and a FEI Quanta-200 with Gatan
3View. Further instruments in the Zentrum for Mikroskopie department
include a Philips Morgani, two Philips CM100, a Zeiss 912 Omega, a
FEI Nova Nano SEM 230, a Philips XL30 FEG ESEM, and a Hitachi S-4800.
These are equipped with energy filters (GIF and Zeiss), CCD cameras
(Gatan, Tietz, Veleta), and cryo-sample holders (Gatan). Sample
preparation devices include a Leica EMPACT2-RTS high-pressure
freezer, cryo-microtomes, freeze substitution devices, and others.


We are looking for an experienced service engineer, to participate in
the general service and maintenance of all these instruments. Tasks
include regular maintenance of the instruments, and repair of the
microscope hardware, vacuum, and electronics systems. In addition,
this engineer will participate in electron microscope hardware
development projects, which involve hardware design, fabrication in
collaboration with our mechanical workshop, construction, and
development of electronic control in collaboration with an electronic
workshop. Upon interest, participation in research projects
concerning structural biology data collection with these instruments
is possible. Our group is a University research group, we develop
instruments and methods, and apply them to study the high-resolution
3D structure of biological specimens. The laboratory language is
English.


The position is permanently funded, and available immediately.


Relocation to the Basel area is required. The Swiss city of Basel is
at the border to Germany and France, and has a flourishing culture,
international flair and an active scientific community.


Further information is available here:
http://www.c-cina.unibas.ch
http://www.basel.ch/en/index
or by email at Henning.Stahlberg-at-unibas.ch or at +41-61-387 3262.


Interested candidates please submit a CV, and a description of
training and experience, and interests by email (PDF preferred) to
Henning.Stahlberg-at-unibas.ch.


Confidentially is assured.



Henning Stahlberg
Center for Cellular Imaging and Nano Analytics (C-CINA)
Structural Biology and Biophysics, Biozentrum,
WRO-1058, Mattenstrasse 26
University Basel, CH-4058 Basel, Switzerland
Tel: +41-61-387 32 62 (office)
Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
Fax: +41-61-387 39 86
mailto:Henning.Stahlberg-at-unibas.ch
Skype:henningstahlberg
http://c-cina.org
http://2dx.org

Postal Address for delivery of Letters / Goods / Express Carriers:
C-CINA, Biozentrum University of Basel
c/o Syngenta AG, WRO-1058.6.60, D-BSSE
Schwarzwaldallee 215
Postfach
CH-4002 Basel


Login Host: 129.132.128.96
---------------------------------------------------------------------------

==============================Original Headers==============================
25, 11 -- From zaluzec-at-microscopy.com Wed Sep 16 09:13:11 2009
25, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
25, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8GED947030400
25, 11 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 09:13:11 -0500
25, 11 -- Mime-Version: 1.0
25, 11 -- Message-Id: {p06240800c6d6a2bfc611-at-[206.69.208.22]}
25, 11 -- Date: Wed, 16 Sep 2009 09:13:08 -0500
25, 11 -- To: microscopy-at-microscopy.com
25, 11 -- From: Henning.Stahlberg-at-unibas.ch (by way of MicroscopyListserver)
25, 11 -- Subject: viaWWW: EM Service Engineer position in Basel, Switzerland
25, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Wed, 16 Sep 2009 11:48:11 -0500
Subject: [Microscopy] Cambridge/LEICA S360 FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,
I'm assisting a gov't customer who took possession of a S360 Field
Emission SEM. Anyone out there have an documentation on this particular
SEM? All of the docs got lost in the move. I'm looking for in
particular any of the procedures concerning the FE tip - Gun bake out,
conditioning, tip replacement, etc., etc.. Please reply to me offline,
Thanks in advance.

Gary Easton
Scanners Corporation
SEM Service
410-857-7633
--


==============================Original Headers==============================
3, 19 -- From garyeaston-at-scannerscorp.com Wed Sep 16 11:48:10 2009
3, 19 -- Received: from omr7.networksolutionsemail.com (omr7.networksolutionsemail.com [205.178.146.57])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8GGmAc7017965
3, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 11:48:10 -0500
3, 19 -- Received: from mail.networksolutionsemail.com (ns-omr7.mgt.netsol.com [10.49.6.70])
3, 19 -- by omr7.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n8GGm8Hq021994
3, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 12:48:10 -0400
3, 19 -- Received: (qmail 23829 invoked by uid 78); 16 Sep 2009 16:48:08 -0000
3, 19 -- Received: from unknown (HELO ?192.168.1.3?) (72.81.181.240)
3, 19 -- by ns-omr7.lb.hosting.dc2.netsol.com with SMTP; 16 Sep 2009 16:48:08 -0000
3, 19 -- Message-ID: {4AB116B2.3050802-at-scannerscorp.com}
3, 19 -- Date: Wed, 16 Sep 2009 12:47:46 -0400
3, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 19 -- MIME-Version: 1.0
3, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 19 -- Subject: Cambridge/LEICA S360 FE
3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Wed, 16 Sep 2009 15:19:29 -0500
Subject: [Microscopy] Info on Oxford EDX interface / system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

We have a customer with a used SEM and Oxford INCA system and are looking for an interface card used by the Oxford INCA - windows 2000 level system back in the early 2000's.

It fits into the PC utilized and is designated the TL-4 "translink" card and we do not know if the card is propriatary or can be bought today. Possibly someone has this, and maybe the translink cable, in a system that has been put out of service and would part with it? Or has the OEM technical info on it to allow us to find one at a reasonable cost?

We also have a Oxford ISIS system with Windows 3.1 that is in our surplus stock, and we would like to upgrade it to Windows 2000 if possible at a low cost.

Please contact me offline if you can help us.

Thank you,

Joe Ullmer

JoeXray LLC
7958 Dubois Road
Carlisle, OHIO 45005
OFFICE / FAX: 937 550-9224
Cell: 937 554-2628
joexray-at-cinci.rr.com
www.joexray.net

==============================Original Headers==============================
8, 19 -- From joexray-at-cinci.rr.com Wed Sep 16 15:19:29 2009
8, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8GKJSXA009712
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 15:19:29 -0500
8, 19 -- Received: from hrndva-web03-z01 ([10.128.132.94])
8, 19 -- by hrndva-smta01.mail.rr.com with ESMTP
8, 19 -- id {20090916201928509.FASB8074-at-hrndva-smta01.mail.rr.com}
8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 20:19:28 +0000
8, 19 -- Message-ID: {20090916201928.R0DKR.136863.root-at-hrndva-web03-z01}
8, 19 -- Date: Wed, 16 Sep 2009 16:19:28 -0400
8, 19 -- From: {joexray-at-cinci.rr.com}
8, 19 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
8, 19 -- Subject: Info on Oxford EDX interface / system
8, 19 -- MIME-Version: 1.0
8, 19 -- Content-Type: text/plain; charset=utf-8
8, 19 -- Content-Transfer-Encoding: 7bit
8, 19 -- X-Priority: 3 (Normal)
8, 19 -- Sensitivity: Normal
8, 19 -- X-Originating-IP:
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Wed, 16 Sep 2009 18:51:03 -0500
Subject: [Microscopy] 2160 lines/mm Mag Cal Sample Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We need to do magnification calibrations on our FEI FIB200.
However, we don't have the 2160 lines/mm Mag Cal sample.
I'm wondering if anyone out there might have one we could borrow?

Thanks for your help!

==============================Original Headers==============================
2, 29 -- From ian.drucker-at-gmail.com Wed Sep 16 18:51:03 2009
2, 29 -- Received: from mail-yw0-f195.google.com (mail-yw0-f195.google.com [209.85.211.195])
2, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8GNp3k2029224
2, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 18:51:03 -0500
2, 29 -- Received: by ywh33 with SMTP id 33so271720ywh.18
2, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 16:51:03 -0700 (PDT)
2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
2, 29 -- d=gmail.com; s=gamma;
2, 29 -- h=domainkey-signature:mime-version:received:from:date:message-id
2, 29 -- :subject:to:content-type;
2, 29 -- bh=aWSHJOFfzIUTsSyu/PVhNNi8y+ptowDcuEuNRVOQEMs=;
2, 29 -- b=GFBLO3BaZ8Tp5dhkaziqZnvm3/BuXWpOzyk2wR+zLqj4Omlb+8oKVgfGfoBWoaabr7
2, 29 -- 0/p0ZRZEwYgRrAUi8GrcgJDsBsdIQaHExiampJVu0ALnD5SGmLFjgb1VuaET1gk90ytR
2, 29 -- 3CH1QT7PiSN2FeaKXd04X6aN+zda5ZtpNsTkg=
2, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
2, 29 -- d=gmail.com; s=gamma;
2, 29 -- h=mime-version:from:date:message-id:subject:to:content-type;
2, 29 -- b=MJL3RRFtPwwnmGzcETPYAuzhrjdOfmAg1HubgvNdIHBmDjn8o12gj+x8zsBtBq8W4J
2, 29 -- S2A7DKnQXaiXaYzL0r4+Ez5Shb9XUF71loc50qo+b6iyPqMzNwtNsad8O9WiuMAIMkJ/
2, 29 -- xmI2F6iuEPakQ05ALgErLi5RLDNIadZcVjR8o=
2, 29 -- MIME-Version: 1.0
2, 29 -- Received: by 10.101.183.7 with SMTP id k7mr7549466anp.164.1253145063098; Wed,
2, 29 -- 16 Sep 2009 16:51:03 -0700 (PDT)
2, 29 -- From: Ian D {ian.drucker-at-gmail.com}
2, 29 -- Date: Wed, 16 Sep 2009 17:50:43 -0600
2, 29 -- Message-ID: {c0bfda950909161650u56dfbbe5y2f670a1fd785a39-at-mail.gmail.com}
2, 29 -- Subject: 2160 lines/mm Mag Cal Sample Needed
2, 29 -- To: Microscopy-at-microscopy.com
2, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: l-reyjr-at-northwestern.edu
Date: Thu, 17 Sep 2009 08:20:26 -0500
Subject: [Microscopy] viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both l-reyjr-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: MSA

Title-Subject: [Filtered] Growing Cells on Grids

Question: Some researchers are interested in growing cells on grids.
Can you provide me with some help?

Thanks
Lennell

Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Sep 17 08:20:25 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HDKOVd007541
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:20:25 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c6d7e802033a-at-[206.69.208.22]}
7, 11 -- Date: Thu, 17 Sep 2009 08:20:23 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Growing Cells on Grids
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Thu, 17 Sep 2009 09:34:15 -0400
Subject: [Microscopy] viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lennell,

I have not done this but worked in a lab where one person did about 15-20 years ago when I was at the Univ. of PA. The reference for this work would have been under Keith Porter. His Research Specialist's first name was Karen I think. The Materials and Methods of his papers may have more clues.

I hope people with more recent knowledge will respond to you but this is what I remember.

You can not use copper grids. They are toxic to the cells. I believe she used 200Mesh gold. See if anyone has old gold grids in their stash. They were thicker than the new ones and much easier to handle IF you can find some.

Clean the grids well, formvar and carbon coat. Then she glow discharged them in a small glass petri dish on a piece of filter paper but used a strip of aluminum instead of the copper wire. We had cut a thin strip off of an alum. sheet, bent it to look like a lasso with a small loop at the end so that we could attach it to the machine where the copper wire had been.

I did not witness the sterilization of the grids but I think she used UV.

Good luck,

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E - Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.



________________________________
X-from: {l-reyjr-at-northwestern.edu}
Reply-To: {l-reyjr-at-northwestern.edu}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both l-reyjr-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: MSA

Title-Subject: [Filtered] Growing Cells on Grids

Question: Some researchers are interested in growing cells on grids.
Can you provide me with some help?

Thanks
Lennell

Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Sep 17 08:20:25 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HDKOVd007541
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:20:25 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c6d7e802033a-at-[206.69.208.22]}
7, 11 -- Date: Thu, 17 Sep 2009 08:20:23 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Growing Cells on Grids
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
28, 30 -- From connellyps-at-nhlbi.nih.gov Thu Sep 17 09:46:03 2009
28, 30 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113])
28, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HEk17p024496
28, 30 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 09:46:02 -0500
28, 30 -- X-IronPortListener: Outbound_SMTP
28, 30 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117])
28, 30 -- by nihxway5out.hub.nih.gov with ESMTP; 17 Sep 2009 10:44:24 -0400
28, 30 -- Received: from NIHHT03.nih.gov ([156.40.71.22]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
28, 30 -- Thu, 17 Sep 2009 10:44:24 -0400
28, 30 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT03.nih.gov
28, 30 -- ([156.40.71.22]) with mapi; Thu, 17 Sep 2009 10:44:23 -0400
28, 30 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
28, 30 -- To: "l-reyjr-at-northwestern.edu" {l-reyjr-at-northwestern.edu}
28, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
28, 30 -- Date: Thu, 17 Sep 2009 10:44:17 -0400
28, 30 -- Subject: Re: [Microscopy] viaWWW: Growing Cells on Grids
28, 30 -- Thread-Topic: [Microscopy] viaWWW: Growing Cells on Grids
28, 30 -- Thread-Index: Aco3m4+k4CUrQ3S8S8afcsXnA0zo9QACcVwR
28, 30 -- Message-ID: {C6D7C381.3F75%connellyps-at-nhlbi.nih.gov}
28, 30 -- In-Reply-To: {200909171334.n8HDYFhh015096-at-ns.microscopy.com}
28, 30 -- Accept-Language: en-US
28, 30 -- Content-Language: en
28, 30 -- X-MS-Has-Attach:
28, 30 -- X-MS-TNEF-Correlator:
28, 30 -- acceptlanguage: en-US
28, 30 -- Content-Type: text/plain; charset="iso-8859-1"
28, 30 -- MIME-Version: 1.0
28, 30 -- X-OriginalArrivalTime: 17 Sep 2009 14:44:24.0121 (UTC) FILETIME=[5952CA90:01CA37A5]
28, 30 -- Content-Transfer-Encoding: 8bit
28, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HEk17p024496
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Thu, 17 Sep 2009 13:29:40 -0500
Subject: [Microscopy] viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I did this about, oh, 25 years ago, as a very young student! I used both
mesh and 1x2mm slot grids, gold, Formvar coated, as Pat said. I can't
remember if I carbon coated. It's possible I coated with poly-L-lysine to
give the cells a start. I was working on crustacean neurons, and I
was a bit frustrated that they grew along the grid bars, which is when I
switched to slot grids.

My main problem was anchoring the grids down in the medium so they
wouldn't float. Can't remember the solution (I'm not being much help,
here), but it's possible I grew some upside down, floating on drops. You
may have to play around a bit to see what makes the cells happy.

The good news is that I was then able to fix, dehydrate, and critical
point dry the neurons on the grids, and then do 400kV TEM and then SEM on
the cells on the grids. I still have them. They were remarkably robust.

Aloha,
Tina

} You can not use copper grids. They are toxic to the cells. I believe
} she used 200Mesh gold. See if anyone has old gold grids in their
} stash. They were thicker than the new ones and much easier to handle
} IF you can find some.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
7, 20 -- From tina-at-pbrc.hawaii.edu Thu Sep 17 13:29:40 2009
7, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HITdSR020450
7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 13:29:40 -0500
7, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
7, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n8HITYLN001893
7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:29:35 -1000 (HST)
7, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n8HITXqc001890
7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:29:34 -1000 (HST)
7, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
7, 20 -- Date: Thu, 17 Sep 2009 08:29:33 -1000 (HST)
7, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
7, 20 -- X-Sender: tina-at-halia
7, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Growing Cells on Grids
7, 20 -- In-Reply-To: {200909171448.n8HEmeNv025968-at-ns.microscopy.com}
7, 20 -- Message-ID: {Pine.GSO.4.21.0909170821220.1724-100000-at-halia}
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 17 Sep 2009 13:53:06 -0500
Subject: [Microscopy] Re: viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Are nickel grids reactive with the culture medium or toxic? If not, one
could use a magnet below the dish to hold Ni grids down......

Cheers,

Dale

tina-at-pbrc.hawaii.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I did this about, oh, 25 years ago, as a very young student! I used both
} mesh and 1x2mm slot grids, gold, Formvar coated, as Pat said. I can't
} remember if I carbon coated. It's possible I coated with poly-L-lysine to
} give the cells a start. I was working on crustacean neurons, and I
} was a bit frustrated that they grew along the grid bars, which is when I
} switched to slot grids.
}
} My main problem was anchoring the grids down in the medium so they
} wouldn't float. Can't remember the solution (I'm not being much help,
} here), but it's possible I grew some upside down, floating on drops. You
} may have to play around a bit to see what makes the cells happy.
}
} The good news is that I was then able to fix, dehydrate, and critical
} point dry the neurons on the grids, and then do 400kV TEM and then SEM on
} the cells on the grids. I still have them. They were remarkably robust.
}
} Aloha,
} Tina
}
} } You can not use copper grids. They are toxic to the cells. I believe
} } she used 200Mesh gold. See if anyone has old gold grids in their
} } stash. They were thicker than the new ones and much easier to handle
} } IF you can find some.
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
} ==============================Original Headers==============================
} 7, 20 -- From tina-at-pbrc.hawaii.edu Thu Sep 17 13:29:40 2009
} 7, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
} 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HITdSR020450
} 7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 13:29:40 -0500
} 7, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
} 7, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n8HITYLN001893
} 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
} 7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:29:35 -1000 (HST)
} 7, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n8HITXqc001890
} 7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:29:34 -1000 (HST)
} 7, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
} 7, 20 -- Date: Thu, 17 Sep 2009 08:29:33 -1000 (HST)
} 7, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} 7, 20 -- X-Sender: tina-at-halia
} 7, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
} 7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Growing Cells on Grids
} 7, 20 -- In-Reply-To: {200909171448.n8HEmeNv025968-at-ns.microscopy.com}
} 7, 20 -- Message-ID: {Pine.GSO.4.21.0909170821220.1724-100000-at-halia}
} 7, 20 -- MIME-Version: 1.0
} 7, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
} ==============================End of - Headers==============================

==============================Original Headers==============================
6, 22 -- From dac-at-research.umass.edu Thu Sep 17 13:53:06 2009
6, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HIr6n5003674
6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 13:53:06 -0500
6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
6, 22 -- (authenticated bits=0)
6, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n8HIr5O8028107
6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 14:53:06 -0400
6, 22 -- Message-ID: {4AB28595.5020400-at-research.umass.edu}
6, 22 -- Date: Thu, 17 Sep 2009 14:53:09 -0400
6, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
6, 22 -- Reply-To: dac-at-research.umass.edu
6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.23) Gecko/20090825 SeaMonkey/1.1.18
6, 22 -- MIME-Version: 1.0
6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
6, 22 -- Subject: Re: [Microscopy] viaWWW: Growing Cells on Grids
6, 22 -- References: {200909171834.n8HIYZXL028422-at-ns.microscopy.com}
6, 22 -- In-Reply-To: {200909171834.n8HIYZXL028422-at-ns.microscopy.com}
6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: filipi-at-pucrs.br
Date: Thu, 17 Sep 2009 16:33:45 -0500
Subject: [Microscopy] Stereo SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi there,

I am doing my masters in computer science and at the virtual reality
class we were asked to make a stereo video. I was wondering if I could
do it with SEM images. I mean, getting enough images, with different
pitch angles to simulate wath the left and right eyes are seeing.

I have found a web site with stereo images from SEM images at
http://emu.arsusda.gov/stereo_mites/default.html

But I want to make a video, as I would be walking around the
specimen.

Does anyone had done it?

Regards,
--
Filipi Vianna
IDEIA/PUCRS Research and Development Institute
+55 51 33203525 ext. 7794
http://www.pucrs.br/ideia


==============================Original Headers==============================
7, 17 -- From filipi-at-pucrs.br Thu Sep 17 16:33:45 2009
7, 17 -- Received: from rigel.pucrs.br (rigel.pucrs.br [201.54.140.13])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HLXg5O023124
7, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 16:33:44 -0500
7, 17 -- Received: from [10.30.46.16] (unknown [10.30.46.16])
7, 17 -- by rigel.pucrs.br (Postfix) with ESMTP id 461D226231
7, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 18:33:34 -0300 (BRT)
7, 17 -- Subject: Stereo SEM images
7, 17 -- From: Filipi Vianna {filipi-at-pucrs.br}
7, 17 -- To: Microscopy-at-microscopy.com
7, 17 -- Content-Type: text/plain; charset="UTF-8"
7, 17 -- Organization: =?ISO-8859-1?Q?ID=C9IA-PUCRS?=
7, 17 -- Date: Thu, 17 Sep 2009 18:33:40 -0300
7, 17 -- Message-Id: {1253223220.12211.5.camel-at-brainstorm}
7, 17 -- Mime-Version: 1.0
7, 17 -- X-Mailer: Evolution 2.24.3
7, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Thu, 17 Sep 2009 17:31:50 -0500
Subject: [Microscopy] viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tina,
Did you leave the grids stuck to the cover-slip when picking up the formvar coating? Removed individual grids after processing? That might help keep the grids sunk in the medium.

Pat
________________________________
X-from: {tina-at-pbrc.hawaii.edu}
Reply-To: {tina-at-pbrc.hawaii.edu}

I think this is what I did - I must have picked up the Formvar-coated gold
grids on a slide and then kept the entire slide/grid/Formvar sandwich
submerged in medium.

Aloha,
Tina

} Tina, Did you leave the grids stuck to the cover-slip when picking up
} the formvar coating? Removed individual grids after processing? That
} might help keep the grids sunk in the medium.
}
} Pat


} I did this about, oh, 25 years ago, as a very young student! I used both
} mesh and 1x2mm slot grids, gold, Formvar coated, as Pat said. I can't
} remember if I carbon coated. It's possible I coated with poly-L-lysine to
} give the cells a start. I was working on crustacean neurons, and I
} was a bit frustrated that they grew along the grid bars, which is when I
} switched to slot grids.
}
} My main problem was anchoring the grids down in the medium so they
} wouldn't float. Can't remember the solution (I'm not being much help,
} here), but it's possible I grew some upside down, floating on drops. You
} may have to play around a bit to see what makes the cells happy.
}
} The good news is that I was then able to fix, dehydrate, and critical
} point dry the neurons on the grids, and then do 400kV TEM and then SEM on
} the cells on the grids. I still have them. They were remarkably robust.
}
} Aloha,
} Tina
}
} } You can not use copper grids. They are toxic to the cells. I believe
} } she used 200Mesh gold. See if anyone has old gold grids in their
} } stash. They were thicker than the new ones and much easier to handle
} } IF you can find some.
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
7, 22 -- From tina-at-pbrc.hawaii.edu Thu Sep 17 17:31:50 2009
7, 22 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HMVnOp020365
7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 17:31:49 -0500
7, 22 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
7, 22 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n8HMVjD5002499
7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 22 -- Thu, 17 Sep 2009 12:31:46 -1000 (HST)
7, 22 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n8HMVgUM002496;
7, 22 -- Thu, 17 Sep 2009 12:31:42 -1000 (HST)
7, 22 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
7, 22 -- Date: Thu, 17 Sep 2009 12:31:41 -1000 (HST)
7, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
7, 22 -- X-Sender: tina-at-halia
7, 22 -- To: l-reyjr-at-northwestern.edu,
7, 22 -- Microscopy Listserver {Microscopy-at-microscopy.com} ,
7, 22 -- connellyps-at-nhlbi.nih.gov
7, 22 -- Subject: Re: [Microscopy] Re: viaWWW: Growing Cells on Grids
7, 22 -- In-Reply-To: {200909172220.n8HMKZKY007222-at-ns.microscopy.com}
7, 22 -- Message-ID: {Pine.GSO.4.21.0909171228340.1896-100000-at-halia}
7, 22 -- MIME-Version: 1.0
7, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: tarawdan-at-ncsu.edu
Date: Thu, 17 Sep 2009 17:47:52 -0500
Subject: [Microscopy] viaWWW: Super-user rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tarawdan-at-ncsu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tarawdan-at-ncsu.edu
Name: Tom Rawdanowicz

Organization: Dept. of Materials Science, NC State University

Title-Subject: [Filtered] Super-user rates

Question: As a manager of a university based TEM
facility operated as a service center, Iíve been
asked to look into the matter of adopting a fee
structure that would consist of two user rates
for the same instrument: a standard hourly rate
for infrequent users and a discounted rate for
frequent ësuperí users. Information has it that
such, or similar user rate structures, currently
exist at other universities.
My immediate thought is this will not pass muster
with the feds re: you can't charge different
users different rates if they are paying with
federal monies. The argument presented to me in
defense of the super user rate is we would be
offering two products, e.g., single quantity vs.
discounted large quantity.
My question for the listserv: is anyone aware of
any similar university base TEM facilities that
have adopted this ìtwo-productî fee structure for
the same instrument and would you happen to know
if theyíve (using this scheme)been audited by the
feds?
Another question for the listserv: what of a
proposal for a user rate system that incorporates
a maximum cap on the total dollar amount per
month for instrument usage, i.e., all users
initially pay the same user rate, but for the
frequent users the total cost for the month is
limited to a set amount. IMHO this still smacks
of different rates for different users.

I've not been able to locate clear and direct
statements in the OMB circular A-21 for a
rebuttal to the aforementioned user rate
proposals.

Your replies are kindly appreciated.

-Tom

Tom Rawdanowicz, PhD, PE
Department of Materials Science & Engineering
NC State University
Raleigh, NC 27695
919.513.0751
tarawdan-at-ncsu.edu


Login Host: 152.14.71.252
---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Thu Sep 17 17:47:51 2009
12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HMlp4c002553
12, 13 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 17:47:51 -0500
12, 13 -- Mime-Version: 1.0
12, 13 -- Message-Id: {p06240800c6d86d0630f3-at-[206.69.208.22]}
12, 13 -- Date: Thu, 17 Sep 2009 17:47:50 -0500
12, 13 -- To: microscopy-at-microscopy.com
12, 13 -- From: tarawdan-at-ncsu.edu (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Super-user rates
12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
12, 13 -- Content-Transfer-Encoding: 8bit
12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HMlp4c002553
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 17 Sep 2009 17:48:50 -0500
Subject: [Microscopy] Re: 2160 lines/mm Mag Cal Sample Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ian

You can purchase this item at any of the usual Electron Microscopy Supply
houses. Just do a Google search on "electron microscopy supplies" you
will find all the major players listed there.

These calibration samples cost ~ $50 and are usually called grating replicas.
They are typically used for low to medium resolution TEM
magnifcation calibration. This is sufficiently inexpensive that
you should just buy your own.

Of course if you need certified magnifications for any ISO work you
will have to purchase a more expensive traceable standard and that will set you
back upwards of $1500+.


Nestor
Your Friendly Neighborhood SysOp









At 6:51 PM -0500 9/16/09, ian.drucker-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
15, 11 -- From zaluzec-at-aaem.amc.anl.gov Thu Sep 17 17:48:50 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HMmnat003964;
15, 11 -- Thu, 17 Sep 2009 17:48:50 -0500
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240801c6d86d2f3a97-at-[206.69.208.22]}
15, 11 -- Date: Thu, 17 Sep 2009 17:48:48 -0500
15, 11 -- To: ian.drucker-at-gmail.com, microscopy-at-microscopy.com
15, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
15, 11 -- Subject: Re: [Microscopy] 2160 lines/mm Mag Cal Sample Needed
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Thu, 17 Sep 2009 18:19:20 -0500
Subject: [Microscopy] Re: viaWWW: Super-user rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom

You can check out the fee structures the University of Sydney charges at the
following link.

http://atomic.emu.usyd.edu.au/emu/index.jsp

As you'll see, the more hours clients buy, the cheaper it gets.

Regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au




On 18/9/09 8:57 AM, "tarawdan-at-ncsu.edu" {tarawdan-at-ncsu.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both tarawdan-at-ncsu.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: tarawdan-at-ncsu.edu
} Name: Tom Rawdanowicz
}
} Organization: Dept. of Materials Science, NC State University
}
} Title-Subject: [Filtered] Super-user rates
}
} Question: As a manager of a university based TEM
} facility operated as a service center, Iíve been
} asked to look into the matter of adopting a fee
} structure that would consist of two user rates
} for the same instrument: a standard hourly rate
} for infrequent users and a discounted rate for
} frequent ësuperí users. Information has it that
} such, or similar user rate structures, currently
} exist at other universities.
} My immediate thought is this will not pass muster
} with the feds re: you can't charge different
} users different rates if they are paying with
} federal monies. The argument presented to me in
} defense of the super user rate is we would be
} offering two products, e.g., single quantity vs.
} discounted large quantity.
} My question for the listserv: is anyone aware of
} any similar university base TEM facilities that
} have adopted this ìtwo-productî fee structure for
} the same instrument and would you happen to know
} if theyíve (using this scheme)been audited by the
} feds?
} Another question for the listserv: what of a
} proposal for a user rate system that incorporates
} a maximum cap on the total dollar amount per
} month for instrument usage, i.e., all users
} initially pay the same user rate, but for the
} frequent users the total cost for the month is
} limited to a set amount. IMHO this still smacks
} of different rates for different users.
}
} I've not been able to locate clear and direct
} statements in the OMB circular A-21 for a
} rebuttal to the aforementioned user rate
} proposals.
}
} Your replies are kindly appreciated.
}
} -Tom
}
} Tom Rawdanowicz, PhD, PE
} Department of Materials Science & Engineering
} NC State University
} Raleigh, NC 27695
} 919.513.0751
} tarawdan-at-ncsu.edu
}
}
} Login Host: 152.14.71.252
} ---------------------------------------------------------------------------
}
}
} ==============================Original Headers==============================
} 12, 13 -- From zaluzec-at-microscopy.com Thu Sep 17 17:47:51 2009
} 12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n8HMlp4c002553
} 12, 13 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 17:47:51 -0500
} 12, 13 -- Mime-Version: 1.0
} 12, 13 -- Message-Id: {p06240800c6d86d0630f3-at-[206.69.208.22]}
} 12, 13 -- Date: Thu, 17 Sep 2009 17:47:50 -0500
} 12, 13 -- To: microscopy-at-microscopy.com
} 12, 13 -- From: tarawdan-at-ncsu.edu (by way of MicroscopyListserver)
} 12, 13 -- Subject: viaWWW: Super-user rates
} 12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} 12, 13 -- Content-Transfer-Encoding: 8bit
} 12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n8HMlp4c002553
} ==============================End of - Headers==============================



==============================Original Headers==============================
17, 29 -- From david.mitchell-at-emu.usyd.edu.au Thu Sep 17 18:19:20 2009
17, 29 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146])
17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HNJJXa031832
17, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 18:19:19 -0500
17, 29 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1])
17, 29 -- by localhost (Postfix) with SMTP id 0994A10397F;
17, 29 -- Fri, 18 Sep 2009 09:19:17 +1000 (EST)
17, 29 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au (ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
17, 29 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id DC9861038C4;
17, 29 -- Fri, 18 Sep 2009 09:19:16 +1000 (EST)
17, 29 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by ex-bri-pro-01.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
17, 29 -- Fri, 18 Sep 2009 09:19:16 +1000
17, 29 -- Received: from 172.17.185.135 ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ;
17, 29 -- Thu, 17 Sep 2009 23:19:16 +0000
17, 29 -- User-Agent: Microsoft-Entourage/11.4.0.080122
17, 29 -- Date: Fri, 18 Sep 2009 09:19:15 +1000
17, 29 -- Subject: Re: [Microscopy] viaWWW: Super-user rates
17, 29 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
17, 29 -- To: {tarawdan-at-ncsu.edu} , {Microscopy-at-microscopy.com}
17, 29 -- Message-ID: {C6D90113.17FE%david.mitchell-at-emu.usyd.edu.au}
17, 29 -- Thread-Topic: [Microscopy] viaWWW: Super-user rates
17, 29 -- Thread-Index: Aco37UW4hCdN3qPgEd6cWgAjMrpsqg==
17, 29 -- In-Reply-To: {200909172257.n8HMvL8V027965-at-ns.microscopy.com}
17, 29 -- Mime-version: 1.0
17, 29 -- Content-type: text/plain;
17, 29 -- charset="ISO-8859-1"
17, 29 -- X-OriginalArrivalTime: 17 Sep 2009 23:19:16.0863 (UTC) FILETIME=[46D520F0:01CA37ED]
17, 29 -- Content-Transfer-Encoding: 8bit
17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HNJJXa031832
==============================End of - Headers==============================




From: rcmoretz-at-gmail.com
Date: Thu, 17 Sep 2009 18:57:25 -0500
Subject: [Microscopy] Re: viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Guess I'll chime in on this one. This goes back 30+ years but I still
know the details. Use either gold or Ti grids, 200 mesh. I never
found nickel to be good in the long run. Formvar films floated on
water were used, then grids placed on the film, picked up with 22mm
square or 18mm round cover slips. After air drying, the formvar was
coated with carbon and the coverslip/grid complex was irradiated with
UV for about 24 hrs for sterilization. The grids were then ready to
be used. Coverslips were placed in petri dishes, seeded with cells
and incubated as usual for the cell types. Cells on the coverslips
were washed with serum depleted medium, fixed with glut and processed
through Os and dehydrated and CPD'd. Grid free areas of the
coverslips were processed for TEM, embedded, and the plastic
(generally) easily separated from the coverslip (due to the formvar).
Fixation times were reduced to 30 min per step and dehydration
schedules were similarly shortened due to the thin nature of the
specimen. I never published anything but the procedure was used by
the Porter group. I know there are publications but I no longer have
any available due to having retired a couple of years ago.

Roger Moretz

On Thu, Sep 17, 2009 at 9:33 AM, {l-reyjr-at-northwestern.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please  copy  both l-reyjr-at-northwestern.edu as well as   the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: l-reyjr-at-northwestern.edu
} Name: Lennell Reynolds
}
} Organization: MSA
}
} Title-Subject: [Filtered] Growing Cells on Grids
}
} Question: Some researchers are interested in growing cells on grids.
} Can you provide me with some help?
}
} Thanks
} Lennell
}
}  Login Host: 165.124.242.201
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Thu Sep 17 08:20:25 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HDKOVd007541
} 7, 11 --        for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 08:20:25 -0500
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240800c6d7e802033a-at-[206.69.208.22]}
} 7, 11 -- Date: Thu, 17 Sep 2009 08:20:23 -0500
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Growing Cells on Grids
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
4, 35 -- From rcmoretz-at-gmail.com Thu Sep 17 18:57:25 2009
4, 35 -- Received: from mail-bw0-f220.google.com (mail-bw0-f220.google.com [209.85.218.220])
4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HNvOq4014592
4, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 18:57:25 -0500
4, 35 -- Received: by bwz20 with SMTP id 20so703385bwz.18
4, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 16:57:24 -0700 (PDT)
4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 35 -- d=gmail.com; s=gamma;
4, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references
4, 35 -- :date:message-id:subject:from:to:content-type
4, 35 -- :content-transfer-encoding;
4, 35 -- bh=5mRpWCyu0yNmt2GsbjAfy/tX9592qGsMuYfNFIXocsU=;
4, 35 -- b=WdRhY981eo5GT5tAqDiMZtYLD9EPnSMqoT2faU2cZhl4bZT293S/fV5IFZT+BgfRJK
4, 35 -- 57UOBRQIes07by62t9o3b2KUjupwnA3Y3cnmb1d9A+Xn3Kg7EOJT3Prvl5OLlwr7s0vL
4, 35 -- p/I8nZSyCEs7EmQYZPJB902SM9pziWNxdRpyQ=
4, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 35 -- d=gmail.com; s=gamma;
4, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
4, 35 -- :content-type:content-transfer-encoding;
4, 35 -- b=E5iZwz5eVuEeAlyP1TWoTgVNK6vEibpDlgggqnb39pWircqlRwiVTxYVXwuBBx9MOq
4, 35 -- j6a9hjOaTc1SHF6MtyLMtsbmzYUa3KwySW/JoaPiKmbGLbZ/FeXN0Je463CtDvnFQddL
4, 35 -- UaRvFhcWGbd4cobKNMJetMw5qs1zLtnhRYexk=
4, 35 -- MIME-Version: 1.0
4, 35 -- Received: by 10.223.60.134 with SMTP id p6mr234812fah.95.1253231843941; Thu,
4, 35 -- 17 Sep 2009 16:57:23 -0700 (PDT)
4, 35 -- In-Reply-To: {200909171333.n8HDX8bd014398-at-ns.microscopy.com}
4, 35 -- References: {200909171333.n8HDX8bd014398-at-ns.microscopy.com}
4, 35 -- Date: Thu, 17 Sep 2009 19:57:23 -0400
4, 35 -- Message-ID: {950e3cfd0909171657i7ce148f8sbd409c1cb60140b5-at-mail.gmail.com}
4, 35 -- Subject: Re: [Microscopy] viaWWW: Growing Cells on Grids
4, 35 -- From: Roger Moretz {rcmoretz-at-gmail.com}
4, 35 -- To: l-reyjr-at-northwestern.edu, Microscopy Listserv {Microscopy-at-microscopy.com}
4, 35 -- Content-Type: text/plain; charset=ISO-8859-1
4, 35 -- Content-Transfer-Encoding: 8bit
4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HNvOq4014592
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Thu, 17 Sep 2009 19:26:34 -0500
Subject: [Microscopy] Re: viaWWW: Super-user rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom,

If you are charging back to federal grants, you can't use a 2-tier
structure. Basically, the government always gets the "best" rate. If
you charge one federal project at one rate and another at a different
rate, the feds aren't getting the "best" rate somewhere along the way.
One possibility is to have ALL users pay a fixed rate up to a certain
quantity, then a lower rate above that amount. It would be more
bookkeeping to keep track of hours below and above the trip point, but I
THINK it would be legal since all the projects have the same rate
structure.

You may need to talk to your sponsored program lawyers to get a more
informed opinion (and to cover yourself).

I think Tina Carvalo (tina-at-pbrc.hawaii.edu) ran into similar issues with
rates a number of years ago at U of Hawaii. She may be able to clarify
the situation.

Cheers,
Henk



tarawdan-at-ncsu.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both tarawdan-at-ncsu.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: tarawdan-at-ncsu.edu
} Name: Tom Rawdanowicz
}
} Organization: Dept. of Materials Science, NC State University
}
} Title-Subject: [Filtered] Super-user rates
}
} Question: As a manager of a university based TEM
} facility operated as a service center, Iíve been
} asked to look into the matter of adopting a fee
} structure that would consist of two user rates
} for the same instrument: a standard hourly rate
} for infrequent users and a discounted rate for
} frequent ësuperí users. Information has it that
} such, or similar user rate structures, currently
} exist at other universities.
} My immediate thought is this will not pass muster
} with the feds re: you can't charge different
} users different rates if they are paying with
} federal monies. The argument presented to me in
} defense of the super user rate is we would be
} offering two products, e.g., single quantity vs.
} discounted large quantity.
} My question for the listserv: is anyone aware of
} any similar university base TEM facilities that
} have adopted this ìtwo-productî fee structure for
} the same instrument and would you happen to know
} if theyíve (using this scheme)been audited by the
} feds?
} Another question for the listserv: what of a
} proposal for a user rate system that incorporates
} a maximum cap on the total dollar amount per
} month for instrument usage, i.e., all users
} initially pay the same user rate, but for the
} frequent users the total cost for the month is
} limited to a set amount. IMHO this still smacks
} of different rates for different users.
}
} I've not been able to locate clear and direct
} statements in the OMB circular A-21 for a
} rebuttal to the aforementioned user rate
} proposals.
}
} Your replies are kindly appreciated.
}
} -Tom
}
} Tom Rawdanowicz, PhD, PE
} Department of Materials Science & Engineering
} NC State University
} Raleigh, NC 27695
} 919.513.0751
} tarawdan-at-ncsu.edu
}
}
} Login Host: 152.14.71.252
} ---------------------------------------------------------------------------
}
}
} ==============================Original Headers==============================
} 12, 13 -- From zaluzec-at-microscopy.com Thu Sep 17 17:47:51 2009
} 12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HMlp4c002553
} 12, 13 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 17:47:51 -0500
} 12, 13 -- Mime-Version: 1.0
} 12, 13 -- Message-Id: {p06240800c6d86d0630f3-at-[206.69.208.22]}
} 12, 13 -- Date: Thu, 17 Sep 2009 17:47:50 -0500
} 12, 13 -- To: microscopy-at-microscopy.com
} 12, 13 -- From: tarawdan-at-ncsu.edu (by way of MicroscopyListserver)
} 12, 13 -- Subject: viaWWW: Super-user rates
} 12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
} 12, 13 -- Content-Transfer-Encoding: 8bit
} 12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HMlp4c002553
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
10, 26 -- From colijn.1-at-osu.edu Thu Sep 17 19:26:34 2009
10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8I0QXEg029677
10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 19:26:34 -0500
10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- id {01NDUFDTB0GW95VPN7-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
10, 26 -- Thu, 17 Sep 2009 20:26:33 -0400 (EDT)
10, 26 -- Received: from [192.168.1.107]
10, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- with ESMTPA id {01NDUFDSUY6095YF0U-at-ecr6.ohio-state.edu} ; Thu,
10, 26 -- 17 Sep 2009 20:26:32 -0400 (EDT)
10, 26 -- Date: Thu, 17 Sep 2009 20:26:32 -0400
10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
10, 26 -- Subject: Re: [Microscopy] viaWWW: Super-user rates
10, 26 -- In-reply-to: {200909172249.n8HMnphc006597-at-ns.microscopy.com}
10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
10, 26 -- To: tarawdan-at-ncsu.edu, microscopy-at-microscopy.com
10, 26 -- Message-id: {4AB2D3B8.3000605-at-osu.edu}
10, 26 -- MIME-version: 1.0
10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
10, 26 -- Content-transfer-encoding: 8BIT
10, 26 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
10, 26 -- References: {200909172249.n8HMnphc006597-at-ns.microscopy.com}
==============================End of - Headers==============================




From: PWebster-at-hei.org
Date: Thu, 17 Sep 2009 23:55:22 -0500
Subject: [Microscopy] Re: viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Why all the old protocols? - growing cells on grids took on a new life with structural cryo-EM. Here is an example:


J Struct Biol. 2008 Mar;161(3):459-68. Epub 2007 Aug 29
Cryo electron tomography of vitrified fibroblasts: microtubule plus ends in situ.
Koning RI, Zovko S, Bárcena M, Oostergetel GT, Koerten HK, Galjart N, Koster AJ, Mieke Mommaas A.


Regards,

Paul Webster
House Ear Institute
2100 W 3rd St
Los Angeles,
CA 90057


==============================Original Headers==============================
7, 20 -- From PWebster-at-hei.org Thu Sep 17 23:55:22 2009
7, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged))
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8I4tMqG022036
7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 23:55:22 -0500
7, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 20 -- Content-class: urn:content-classes:message
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain;
7, 20 -- charset="iso-8859-1"
7, 20 -- Subject: Re: viaWWW: Growing Cells on Grids
7, 20 -- Date: Thu, 17 Sep 2009 21:55:21 -0700
7, 20 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F830814D1B4-at-hi0sml1.hei.org}
7, 20 -- X-MS-Has-Attach:
7, 20 -- X-MS-TNEF-Correlator:
7, 20 -- Thread-Topic: viaWWW: Growing Cells on Grids
7, 20 -- Thread-Index: Aco4HDoF9xZhj+s4Rue4EgusPPgwrw==
7, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
7, 20 -- To: {microscopy-at-microscopy.com}
7, 20 -- Content-Transfer-Encoding: 8bit
7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8I4tMqG022036
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Fri, 18 Sep 2009 00:30:03 -0500
Subject: [Microscopy] Re: Stereo SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Filipi,
I thought of this also some years ago, but it seems a lot of work with
the scope...
At least you need a scope with all motor movements controlled.
Using the right scope you are half way down the road:
There is a manufacturer called TESCAN who is (with his new scopes like
Mira) able to produce 3D images on the fly with tilding the beam, not
the specimen.
Maybe you can get access to such a scope and then have only the problem
remaining controlling the movement of the specimen :-)
Keep us posted.

Best wishes,
Stefan



filipi-at-pucrs.br schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi there,
}
} I am doing my masters in computer science and at the virtual reality
} class we were asked to make a stereo video. I was wondering if I could
} do it with SEM images. I mean, getting enough images, with different
} pitch angles to simulate wath the left and right eyes are seeing.
}
} I have found a web site with stereo images from SEM images at
} http://emu.arsusda.gov/stereo_mites/default.html
}
} But I want to make a video, as I would be walking around the
} specimen.
}
} Does anyone had done it?
}
} Regards,

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Fri Sep 18 00:30:03 2009
7, 22 -- Received: from mailout10.t-online.de (mailout10.t-online.de [194.25.134.21])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8I5U2XD004979
7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 00:30:02 -0500
7, 22 -- Received: from fwd07.aul.t-online.de
7, 22 -- by mailout10.t-online.de with smtp
7, 22 -- id 1MoW2J-000479-01; Fri, 18 Sep 2009 07:29:35 +0200
7, 22 -- Received: from [192.168.2.101] (Ek7uSqZfQhhPUrr7ZsHXbBo6EG2kntdU9Ls9kxYCaRl48xSYj3tQcBvzbtaMc6ywMv-at-[93.222.125.241]) by fwd07.aul.t-online.de
7, 22 -- with esmtp id 1MoW28-1ABKy00; Fri, 18 Sep 2009 07:29:24 +0200
7, 22 -- Message-ID: {4AB31AB3.1070106-at-t-online.de}
7, 22 -- Date: Fri, 18 Sep 2009 07:29:23 +0200
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: [Microscopy] Stereo SEM images
7, 22 -- References: {200909172138.n8HLcZfO029866-at-ns.microscopy.com}
7, 22 -- In-Reply-To: {200909172138.n8HLcZfO029866-at-ns.microscopy.com}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID: Ek7uSqZfQhhPUrr7ZsHXbBo6EG2kntdU9Ls9kxYCaRl48xSYj3tQcBvzbtaMc6ywMv
7, 22 -- X-TOI-MSGID: 064f6097-e4f7-48dd-8edf-4d353adadc59
==============================End of - Headers==============================




From: donk-at-ardl.com
Date: Fri, 18 Sep 2009 07:25:15 -0500
Subject: [Microscopy] RE: 2160 line Calibration Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor, I use the 2160 line/mm calibration standard and was told some time ago by Ted Pella that there is no traceable standard per se. All standards are traceable to 4 master standards used to make the replicas. Where do you obtain one of these $1,500.00 traceable standards? As far as I'm concerned all of them are "replicas" and are traceable to the Masters, so you're just paying them $1,450.00 for nothing.

Don Kierstead
Microscopist
ARDL, Inc.
www.ardl.com
(866) 778-ARDL [toll free]
(330) 794-6600 [worldwide]
(330) 794-6610 [fax]



This email and any of its attachments may contain confidential information
intended only for the use of the addressee(s). If the reader of this email
is not the intended recipient or the employee or agent responsible for
delivering it to the intended recipient, you are hereby notified that any
dissemination or copying of this email is strictly prohibited. If you have
received this email in error, please notify us by return email at
info-at-ardl.com, permanently delete the email, and destroy any printouts. If
this email contains test data and/or draft reports, you are hereby notified
that only a signed original test report will contain official results, a
copy of which resides in the project folder located at ARDL, Inc. Thank
you. Akron Rubber Development Laboratory, Inc.



==============================Original Headers==============================
7, 20 -- From donk-at-ardl.com Fri Sep 18 07:25:15 2009
7, 20 -- Received: from exchange2k.ad.ardl.com (mail2.ardl.com [64.179.51.38])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ICPFmV001200
7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 07:25:15 -0500
7, 20 -- Content-Class: urn:content-classes:message
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain;
7, 20 -- charset="iso-8859-1"
7, 20 -- Subject: RE: 2160 line Calibration Standard
7, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1983
7, 20 -- Date: Fri, 18 Sep 2009 08:25:14 -0400
7, 20 -- Message-ID: {BDA0AFAE50B38B438475725B8FA5120D06765EAC-at-exchange2k.ad.ardl.com}
7, 20 -- X-MS-Has-Attach:
7, 20 -- X-MS-TNEF-Correlator:
7, 20 -- Thread-Topic: RE: 2160 line Calibration Standard
7, 20 -- thread-index: Aco4WxMgl0ORIClfRDqcXfMl0T11zw==
7, 20 -- From: "Don Kierstead" {donk-at-ardl.com}
7, 20 -- To: "Microscopy Listserver \(E-mail\)" {Microscopy-at-microscopy.com}
7, 20 -- Content-Transfer-Encoding: 8bit
7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8ICPFmV001200
==============================End of - Headers==============================




From: Woody.White-at-areva.com
Date: Fri, 18 Sep 2009 08:31:54 -0500
Subject: [Microscopy] RE: 2160 line Calibration Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don,

If you need a tracable mag standard, NIST has recently released a new
one, SRM-8820, that may interest you.
The price is quite reasonable for this sort of thing too.
The specs:
https://www-s.nist.gov/srmors/certificates/8820.pdf?CFID=823061&CFTOKEN=
d94e2276845da8d8-CD52B2AF-963A-E105-2586DA6F1D858C42&jsessionid=f030c53d
ee62fc88edb26760d194d175d646

Woody

N.W. (Woody) White Jr.
Electron Microscopist
Chemistry and Materials Center
AREVA NP Inc
An AREVA and Siemens Company
Lynchburg, VA
Lab Phone: 434.832.3004



-----Original Message-----
X-from: donk-at-ardl.com [mailto:donk-at-ardl.com]
Sent: Friday, September 18, 2009 8:34 AM
To: WHITE Norvell (AREVA NP INC)

Nestor, I use the 2160 line/mm calibration standard and was told some
time ago by Ted Pella that there is no traceable standard per se. All
standards are traceable to 4 master standards used to make the replicas.
Where do you obtain one of these $1,500.00 traceable standards? As far
as I'm concerned all of them are "replicas" and are traceable to the
Masters, so you're just paying them $1,450.00 for nothing.

Don Kierstead
Microscopist
ARDL, Inc.
www.ardl.com
(866) 778-ARDL [toll free]
(330) 794-6600 [worldwide]
(330) 794-6610 [fax]



This email and any of its attachments may contain confidential
information intended only for the use of the addressee(s). If the reader
of this email is not the intended recipient or the employee or agent
responsible for delivering it to the intended recipient, you are hereby
notified that any dissemination or copying of this email is strictly
prohibited. If you have received this email in error, please notify us
by return email at info-at-ardl.com, permanently delete the email, and
destroy any printouts. If this email contains test data and/or draft
reports, you are hereby notified that only a signed original test report
will contain official results, a copy of which resides in the project
folder located at ARDL, Inc. Thank you. Akron Rubber Development
Laboratory, Inc.



==============================Original
Headers==============================
7, 20 -- From donk-at-ardl.com Fri Sep 18 07:25:15 2009 7, 20 -- Received:
from exchange2k.ad.ardl.com (mail2.ardl.com [64.179.51.38])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n8ICPFmV001200
7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009
07:25:15 -0500
7, 20 -- Content-Class: urn:content-classes:message 7, 20 --
MIME-Version: 1.0 7, 20 -- Content-Type: text/plain;
7, 20 -- charset="iso-8859-1"
7, 20 -- Subject: RE: 2160 line Calibration Standard 7, 20 --
X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1983 7, 20 -- Date:
Fri, 18 Sep 2009 08:25:14 -0400 7, 20 -- Message-ID:
{BDA0AFAE50B38B438475725B8FA5120D06765EAC-at-exchange2k.ad.ardl.com}
7, 20 -- X-MS-Has-Attach:
7, 20 -- X-MS-TNEF-Correlator:
7, 20 -- Thread-Topic: RE: 2160 line Calibration Standard 7, 20 --
thread-index: Aco4WxMgl0ORIClfRDqcXfMl0T11zw== 7, 20 -- From: "Don
Kierstead" {donk-at-ardl.com} 7, 20 -- To: "Microscopy Listserver
\(E-mail\)" {Microscopy-at-microscopy.com} 7, 20 --
Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from
quoted-printable to 8bit by ns.microscopy.com id n8ICPFmV001200
==============================End of -
Headers==============================


==============================Original Headers==============================
19, 30 -- From Woody.White-at-areva.com Fri Sep 18 08:31:52 2009
19, 30 -- Received: from smtpout73.framatome-anp.us (smtpout73.framatome-anp.us [160.84.253.73])
19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IDVqKj018644
19, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 08:31:52 -0500
19, 30 -- Received: from RUSAHUBBX01.ad.corp (rusahubbx01.ad.corp [10.2.161.110])
19, 30 -- by smtpout73.framatome-anp.us (Postfix) with ESMTP id 439AC26C038
19, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 13:31:52 +0000 (UTC)
19, 30 -- Received: from auslyncbx04.adom.ad.corp ([160.84.193.82]) by RUSAHUBBX01.ad.corp with Microsoft SMTPSVC(6.0.3790.3959);
19, 30 -- Fri, 18 Sep 2009 09:31:52 -0400
19, 30 -- Received: from AUSLYNCMX02.adom.ad.corp ([160.84.193.182]) by auslyncbx04.adom.ad.corp with Microsoft SMTPSVC(6.0.3790.3959);
19, 30 -- Fri, 18 Sep 2009 09:31:52 -0400
19, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
19, 30 -- Content-class: urn:content-classes:message
19, 30 -- MIME-Version: 1.0
19, 30 -- Content-Type: text/plain;
19, 30 -- charset="us-ascii"
19, 30 -- Subject: RE: [Microscopy] RE: 2160 line Calibration Standard
19, 30 -- Date: Fri, 18 Sep 2009 09:31:51 -0400
19, 30 -- Message-ID: {BC417D9255991046A37DD56CF597DB7103D4D672-at-AUSLYNCMX02.adom.ad.corp}
19, 30 -- In-Reply-To: {200909181233.n8ICXlAg014554-at-ns.microscopy.com}
19, 30 -- X-MS-Has-Attach:
19, 30 -- X-MS-TNEF-Correlator:
19, 30 -- Thread-Topic: [Microscopy] RE: 2160 line Calibration Standard
19, 30 -- Thread-Index: Aco4XEWdFKyKFdFQQdquXEwtmU/d7gAB1AAw
19, 30 -- References: {200909181233.n8ICXlAg014554-at-ns.microscopy.com}
19, 30 -- From: "WHITE Norvell (AREVA NP INC)" {Woody.White-at-areva.com}
19, 30 -- To: {Microscopy-at-microscopy.com}
19, 30 -- X-OriginalArrivalTime: 18 Sep 2009 13:31:52.0031 (UTC) FILETIME=[61B04EF0:01CA3864]
19, 30 -- Content-Transfer-Encoding: 8bit
19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8IDVqKj018644
==============================End of - Headers==============================




From: smithj-at-winthrop.edu
Date: Fri, 18 Sep 2009 08:52:08 -0500
Subject: [Microscopy] SEM service contract in the southeastern US?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have an ISI Super IIIA SEM with attached 4Pi x-ray system and digital
imaging system, and we need a service contract (MAS, Inc has provided
excellent service on this instrument over the years, but they are
getting out of the instrument service-contract business).
If you are interested in providing a contract on this instrument, please
contact me directly via e-mail (smithj-at-winthrop.edu).
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)


==============================Original Headers==============================
4, 18 -- From smithj-at-winthrop.edu Fri Sep 18 08:52:08 2009
4, 18 -- Received: from class.winthrop.edu (class.winthrop.edu [199.79.254.233])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IDq67x001625
4, 18 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 08:52:06 -0500
4, 18 -- Received: from berlin.win.winthrop.edu [10.2.0.22] by class.winthrop.edu with ESMTP
4, 18 -- (SMTPD32-8.14) id A084AA500BE; Fri, 18 Sep 2009 09:52:04 -0400
4, 18 -- Received: from Julian-Smiths-Computer.local ([10.3.84.9]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959);
4, 18 -- Fri, 18 Sep 2009 09:52:04 -0400
4, 18 -- Message-ID: {4AB39084.3090305-at-winthrop.edu}
4, 18 -- Date: Fri, 18 Sep 2009 09:52:04 -0400
4, 18 -- From: Julian Smith III {smithj-at-winthrop.edu}
4, 18 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
4, 18 -- MIME-Version: 1.0
4, 18 -- To: microscopy-at-microscopy.com
4, 18 -- Subject: SEM service contract in the southeastern US?
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
4, 18 -- X-OriginalArrivalTime: 18 Sep 2009 13:52:04.0620 (UTC) FILETIME=[3472B4C0:01CA3867]
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Fri, 18 Sep 2009 08:59:26 -0500
Subject: [Microscopy] Re: 2160 lines/mm Mag Cal Sample Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ian & Listers,

Note that Ian needed this standard for a FIB200. The ion beam will
destroy a standard TEM grating replica in short order.

Ian, go to any of the EM supply houses and look for calibration
standards. Pretty much any of the SEM mag cal standards should work
for you as long as the dimensions are of the order you need. You
don't necessarily need 2160 line/mm to do the calibration.

On a related issue... Does anyone know how the 2160 line/mm TEM
gratings are prepared? If I had to guess, I would think that it is a
cellulose acetate replica of a diffraction grating that is then Au
shadowed, carbon coated, and washed clean. If so, the grating
replica is necessarily a secondary standard because of questions
about shrinkage of the acetate replica during processing. Does
anyone have uncertainty values?

Cheers
Henk

At 07:52 PM 09/16/09, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
11, 26 -- From colijn.1-at-osu.edu Fri Sep 18 08:59:26 2009
11, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IDxPw5013246
11, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 08:59:26 -0500
11, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
11, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
11, 26 -- id {01NDV7RLENCW95USIE-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
11, 26 -- Fri, 18 Sep 2009 09:59:24 -0400 (EDT)
11, 26 -- Received: from HOC1.ecr6.ohio-state.edu
11, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
11, 26 -- (PMDF V6.3-x18 #31556)
11, 26 -- with ESMTPA id {01NDV7RKXYG695YBRU-at-ecr6.ohio-state.edu} ; Fri,
11, 26 -- 18 Sep 2009 09:59:23 -0400 (EDT)
11, 26 -- Date: Fri, 18 Sep 2009 10:01:38 -0400
11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
11, 26 -- Subject: Re: [Microscopy] 2160 lines/mm Mag Cal Sample Needed
11, 26 -- In-reply-to: {200909162352.n8GNqwM6031373-at-ns.microscopy.com}
11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
11, 26 -- To: ian.drucker-at-gmail.com
11, 26 -- Cc: Microscopy-at-microscopy.com
11, 26 -- Message-id: {01NDV7RL20FS95YBRU-at-ecr6.ohio-state.edu}
11, 26 -- MIME-version: 1.0
11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
11, 26 -- References: {200909162352.n8GNqwM6031373-at-ns.microscopy.com}
==============================End of - Headers==============================




From: Phil.Ahrenkiel-at-sdsmt.edu
Date: Fri, 18 Sep 2009 09:30:48 -0500
Subject: [Microscopy] viaWWW: Super-user rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Over the past three years, we have used an annual subscription approach, where subscribing users who pay over $1000 during the subscription "drive" get an additional 25% match from the lab toward use of the equipment. The matching portion expires after one year. I have also offered subscribers some free sample prep, or unlimited use of our heating stage, which was purchased with state funds. Subscribers are also allowed to sponsor limited use of the equipment to support new proposals at no cost. The subscriber approach helps to draw in a large pool to refresh the account for purchasing the service contract, which cannot typically be met by whatever balance remains in the account. (To give myself some credit, the balance was negative when I got here, so things have improved.)

However, we ran into a problem this year with our accounting office, which does not permit pre-payment for services. The result is that our program has had to pay for the service contract, so nobody gets a discount, but we can't continue this indefinitely. I think the subscriber system makes sense, in principle, because it encourages greater use of the equipment. Unless the university commits to a fund for buying service contracts, the obvious source is grants, and a discounted rate or match give the users some incentive to commit their funds in advance. But I would not charge different rates based solely on frequency of use.
------------------------------------------
Phil Ahrenkiel, Assistant Professor
Nanoscience and Nanoengineering Ph.D. Program
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701
Office: EP 221
Phone: 605-394-5238, Fax: 605-394-2365
Email: Phil.Ahrenkiel-at-sdsmt.edu

-----Original Message-----
X-from: tarawdan-at-ncsu.edu [mailto:tarawdan-at-ncsu.edu]
Sent: Thursday, September 17, 2009 4:53 PM
To: Ahrenkiel, Phil

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both tarawdan-at-ncsu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: tarawdan-at-ncsu.edu
Name: Tom Rawdanowicz

Organization: Dept. of Materials Science, NC State University

Title-Subject: [Filtered] Super-user rates

Question: As a manager of a university based TEM
facility operated as a service center, Iíve been
asked to look into the matter of adopting a fee
structure that would consist of two user rates
for the same instrument: a standard hourly rate
for infrequent users and a discounted rate for
frequent ësuperí users. Information has it that
such, or similar user rate structures, currently
exist at other universities.
My immediate thought is this will not pass muster
with the feds re: you can't charge different
users different rates if they are paying with
federal monies. The argument presented to me in
defense of the super user rate is we would be
offering two products, e.g., single quantity vs.
discounted large quantity.
My question for the listserv: is anyone aware of
any similar university base TEM facilities that
have adopted this ìtwo-productî fee structure for
the same instrument and would you happen to know
if theyíve (using this scheme)been audited by the
feds?
Another question for the listserv: what of a
proposal for a user rate system that incorporates
a maximum cap on the total dollar amount per
month for instrument usage, i.e., all users
initially pay the same user rate, but for the
frequent users the total cost for the month is
limited to a set amount. IMHO this still smacks
of different rates for different users.

I've not been able to locate clear and direct
statements in the OMB circular A-21 for a
rebuttal to the aforementioned user rate
proposals.

Your replies are kindly appreciated.

-Tom

Tom Rawdanowicz, PhD, PE
Department of Materials Science & Engineering
NC State University
Raleigh, NC 27695
919.513.0751
tarawdan-at-ncsu.edu


Login Host: 152.14.71.252
---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Thu Sep 17 17:47:51 2009
12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8HMlp4c002553
12, 13 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2009 17:47:51 -0500
12, 13 -- Mime-Version: 1.0
12, 13 -- Message-Id: {p06240800c6d86d0630f3-at-[206.69.208.22]}
12, 13 -- Date: Thu, 17 Sep 2009 17:47:50 -0500
12, 13 -- To: microscopy-at-microscopy.com
12, 13 -- From: tarawdan-at-ncsu.edu (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Super-user rates
12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
12, 13 -- Content-Transfer-Encoding: 8bit
12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8HMlp4c002553
==============================End of - Headers==============================


==============================Original Headers==============================
20, 25 -- From Phil.Ahrenkiel-at-sdsmt.edu Fri Sep 18 09:30:47 2009
20, 25 -- Received: from sdsmt-ex07.sdsmt.edu (sdsmt-ex07.sdsmt.edu [151.159.3.6])
20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IEUltX032260
20, 25 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 09:30:47 -0500
20, 25 -- Received: from sdsmt-ex07.SDSMT.local ([151.159.3.6]) by sdsmt-ex07
20, 25 -- ([151.159.3.6]) with mapi; Fri, 18 Sep 2009 08:30:44 -0600
20, 25 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu}
20, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
20, 25 -- CC: "tarawdan-at-ncsu.edu" {tarawdan-at-ncsu.edu}
20, 25 -- Date: Fri, 18 Sep 2009 08:30:43 -0600
20, 25 -- Subject: RE: [Microscopy] viaWWW: Super-user rates
20, 25 -- Thread-Topic: [Microscopy] viaWWW: Super-user rates
20, 25 -- Thread-Index: Aco36a+FjoozOHWcS5KHuZfPuILEWQAf9xLQ
20, 25 -- Message-ID: {0E8C875976EF6C4FAD2BE0CEE93BD2D603DBCF3DB4-at-sdsmt-ex07}
20, 25 -- References: {200909172253.n8HMrRbY017364-at-ns.microscopy.com}
20, 25 -- In-Reply-To: {200909172253.n8HMrRbY017364-at-ns.microscopy.com}
20, 25 -- Accept-Language: en-US
20, 25 -- Content-Language: en-US
20, 25 -- X-MS-Has-Attach:
20, 25 -- X-MS-TNEF-Correlator:
20, 25 -- acceptlanguage: en-US
20, 25 -- Content-Type: text/plain; charset="iso-8859-1"
20, 25 -- MIME-Version: 1.0
20, 25 -- Content-Transfer-Encoding: 8bit
20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8IEUltX032260
==============================End of - Headers==============================




From: ian.drucker-at-gmail.com
Date: Fri, 18 Sep 2009 11:51:40 -0500
Subject: [Microscopy] Re: 2160 lines/mm Mag Cal Sample Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I ordered one through www.tedpella.com for about $55. I didn't
initially find any when I did a search except at EMS for $550, thus
why I made the request.

Thanks for all the replies.

On Fri, Sep 18, 2009 at 8:01 AM, Hendrik O. Colijn {colijn.1-at-osu.edu} wrote:
} Hi Ian & Listers,
}
} Note that Ian needed this standard for a FIB200.  The ion beam will destroy
} a standard TEM grating replica in short order.
}
} Ian,  go to any of the EM supply houses and look for calibration standards.
}  Pretty much any of the SEM mag cal standards should work for you as long as
} the dimensions are of the order you need.  You don't necessarily need 2160
} line/mm to do the calibration.
}
} On a related issue...  Does anyone know how the 2160 line/mm TEM gratings
} are prepared?  If I had to guess, I would think that it is a cellulose
} acetate replica of a diffraction grating that is then Au shadowed, carbon
} coated, and washed clean.  If so, the grating replica is necessarily a
} secondary standard because of questions about shrinkage of the acetate
} replica during processing.  Does anyone have uncertainty values?
}
} Cheers
} Henk
}
} At 07:52 PM 09/16/09, you wrote:
}
}
}
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} } ----------------------------------------------------------------------------
} }
} } We need to do magnification calibrations on our FEI FIB200.
} } However, we don't have the 2160 lines/mm Mag Cal sample.
} } I'm wondering if anyone out there might have one we could borrow?
} }
} } Thanks for your help!
} }
} } ==============================Original
} } Headers==============================
} } 2, 29 -- From ian.drucker-at-gmail.com Wed Sep 16 18:51:03 2009
} } 2, 29 -- Received: from mail-yw0-f195.google.com (mail-yw0-f195.google.com
} } [209.85.211.195])
} } 2, 29 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} } id n8GNp3k2029224
} } 2, 29 --        for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009 18:51:03
} } -0500
} } 2, 29 -- Received: by ywh33 with SMTP id 33so271720ywh.18
} } 2, 29 --         for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2009
} } 16:51:03 -0700 (PDT)
} } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} } 2, 29 --         d=gmail.com; s=gamma;
} } 2, 29 --
} } h=domainkey-signature:mime-version:received:from:date:message-id
} } 2, 29 --          :subject:to:content-type;
} } 2, 29 --         bh=aWSHJOFfzIUTsSyu/PVhNNi8y+ptowDcuEuNRVOQEMs=;
} } 2, 29 --
} } b=GFBLO3BaZ8Tp5dhkaziqZnvm3/BuXWpOzyk2wR+zLqj4Omlb+8oKVgfGfoBWoaabr7
} } 2, 29
} } --
} } 0/p0ZRZEwYgRrAUi8GrcgJDsBsdIQaHExiampJVu0ALnD5SGmLFjgb1VuaET1gk90ytR
} } 2, 29 --          3CH1QT7PiSN2FeaKXd04X6aN+zda5ZtpNsTkg=
} } 2, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} } 2, 29 --         d=gmail.com; s=gamma;
} } 2, 29 --
} } h=mime-version:from:date:message-id:subject:to:content-type;
} } 2, 29 --
} } b=MJL3RRFtPwwnmGzcETPYAuzhrjdOfmAg1HubgvNdIHBmDjn8o12gj+x8zsBtBq8W4J
} } 2, 29
} } --
} } S2A7DKnQXaiXaYzL0r4+Ez5Shb9XUF71loc50qo+b6iyPqMzNwtNsad8O9WiuMAIMkJ/
} } 2, 29 --          xmI2F6iuEPakQ05ALgErLi5RLDNIadZcVjR8o=
} } 2, 29 -- MIME-Version: 1.0
} } 2, 29 -- Received: by 10.101.183.7 with SMTP id
} } k7mr7549466anp.164.1253145063098; Wed,
} } 2, 29 --        16 Sep 2009 16:51:03 -0700 (PDT)
} } 2, 29 -- From: Ian D {ian.drucker-at-gmail.com}
} } 2, 29 -- Date: Wed, 16 Sep 2009 17:50:43 -0600
} } 2, 29 -- Message-ID:
} } {c0bfda950909161650u56dfbbe5y2f670a1fd785a39-at-mail.gmail.com}
} } 2, 29 -- Subject: 2160 lines/mm Mag Cal Sample Needed
} } 2, 29 -- To: Microscopy-at-microscopy.com
} } 2, 29 -- Content-Type: text/plain; charset=ISO-8859-1
} } ==============================End of -
} } Headers==============================
}
} Hendrik O. Colijn                       colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674                  http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all at
} once. Lately it doesn't seem to be working.
}


==============================Original Headers==============================
4, 37 -- From ian.drucker-at-gmail.com Fri Sep 18 11:51:39 2009
4, 37 -- Received: from mail-yx0-f189.google.com (mail-yx0-f189.google.com [209.85.210.189])
4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IGpdLO020286
4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 11:51:39 -0500
4, 37 -- Received: by yxe27 with SMTP id 27so1450880yxe.10
4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 09:51:39 -0700 (PDT)
4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 37 -- d=gmail.com; s=gamma;
4, 37 -- h=domainkey-signature:mime-version:received:in-reply-to:references
4, 37 -- :from:date:message-id:subject:to:cc:content-type
4, 37 -- :content-transfer-encoding;
4, 37 -- bh=+Ee+vFcmVD/YRiNHMfhNN7z1j6ZUs0of0ihuuRvh2Tw=;
4, 37 -- b=elNGq2mstVt7GQfQYrDosae/BZ224K7TvacE1TGMLJmXnciitQIHf73Y6Svxw3qaDr
4, 37 -- uijSKBahzyff49gro3asIc4QbAfIfOy3TM3KQ2dX3gJF5vPaTJSiKK+wYEPaPgwpfqFY
4, 37 -- tF6N0XjYz3S8vVrn5LvSZW5ells6CL7SnF93c=
4, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 37 -- d=gmail.com; s=gamma;
4, 37 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to
4, 37 -- :cc:content-type:content-transfer-encoding;
4, 37 -- b=DNDdn5RFVN8J6VlWirgMDb7AEyzmdQayJlickoPnvrHL5b3o++0E4IOKvT1TZ0/8En
4, 37 -- zKxfEqR4lSon5rQbj95j4uXiNTMjd7zp9zIBJw+1R1PA5+6aVQDJzQ2MWHAhtnL7D8x2
4, 37 -- MiQeFkQcaBmtskBeyZ+AV/R0aRT3vVRoU4n9w=
4, 37 -- MIME-Version: 1.0
4, 37 -- Received: by 10.101.90.4 with SMTP id s4mr1673384anl.159.1253292699210; Fri,
4, 37 -- 18 Sep 2009 09:51:39 -0700 (PDT)
4, 37 -- In-Reply-To: {01NDV7RL20FS95YBRU-at-ecr6.ohio-state.edu}
4, 37 -- References: {200909162352.n8GNqwM6031373-at-ns.microscopy.com}
4, 37 -- {01NDV7RL20FS95YBRU-at-ecr6.ohio-state.edu}
4, 37 -- From: Ian D {ian.drucker-at-gmail.com}
4, 37 -- Date: Fri, 18 Sep 2009 10:51:19 -0600
4, 37 -- Message-ID: {c0bfda950909180951j496a62f2w843e28c52b58aa89-at-mail.gmail.com}
4, 37 -- Subject: Re: [Microscopy] 2160 lines/mm Mag Cal Sample Needed
4, 37 -- To: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
4, 37 -- Cc: Microscopy-at-microscopy.com
4, 37 -- Content-Type: text/plain; charset=ISO-8859-1
4, 37 -- Content-Transfer-Encoding: 8bit
4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8IGpdLO020286
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 18 Sep 2009 12:33:22 -0500
Subject: [Microscopy] Re: viaWWW: Growing Cells on Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ancient History:

We published a technique to grow cells (bacteria, in our case) on a
variety of surfaces, including sterilized TEM grids. It worked
beautifully and should work in your situation. You would only need to
substitute gold-coated grids. These are not solid gold grids, but
copper grids that have been electrolytically coated with gold. The
coated grids are less fragile than solid gold. I believe you can
obtain them from Electron MIcroscopy Sciences (and possibly other
vendors).

Here is the reference:

Bozzola JJ, Johnson MC, Shechmeister IL. 1973. In situ multiple
sampling of attached bacteria for scanning and transmission electron
microscopy. Stain Technol. Nov 48(6):317-25.

I have a copy that I can email to anyone who might be interested


--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901

==============================Original Headers==============================
7, 17 -- From bozzola-at-siu.edu Fri Sep 18 12:33:22 2009
7, 17 -- Received: from mail-pz0-f182.google.com (mail-pz0-f182.google.com [209.85.222.182])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IHXLpU003578
7, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 12:33:21 -0500
7, 17 -- Received: by pzk12 with SMTP id 12so898733pzk.10
7, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 10:33:21 -0700 (PDT)
7, 17 -- MIME-Version: 1.0
7, 17 -- Received: by 10.115.65.5 with SMTP id s5mr2830341wak.41.1253295201181; Fri, 18
7, 17 -- Sep 2009 10:33:21 -0700 (PDT)
7, 17 -- In-Reply-To: {200909172232.n8HMWT1j021641-at-ns.microscopy.com}
7, 17 -- References: {200909172232.n8HMWT1j021641-at-ns.microscopy.com}
7, 17 -- Date: Fri, 18 Sep 2009 12:33:21 -0500
7, 17 -- Message-ID: {ebc2299e0909181033o65a4eaeaidd26fac02d19efba-at-mail.gmail.com}
7, 17 -- Subject: Re: [Microscopy] viaWWW: Growing Cells on Grids
7, 17 -- From: John Bozzola {bozzola-at-siu.edu}
7, 17 -- To: MSAListserver {Microscopy-at-microscopy.com}
7, 17 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Fri, 18 Sep 2009 13:55:54 -0500
Subject: [Microscopy] Cambridge S-90B SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

I have a customer that bought a used Cambrdige S-90B SEM. They did not get a set of schematics or any service manuals with it, and we need to secure a set to allow their technician to keep it up and operational.

Does anyone out there have a set that they could spare or allow us to copy?

Please contact me offline if you could possibly help us in this regard.

Thank you,

Joe Ullmer

JoeXray LLC
7958 Dubois Road
Carlisle, OHIO 45005
OFFICE / FAX: 937 550-9224
Cell: 937 554-2628
joexray-at-cinci.rr.com
www.joexray.net

==============================Original Headers==============================
7, 19 -- From joexray-at-cinci.rr.com Fri Sep 18 13:55:54 2009
7, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123])
7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IItsUD020367
7, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 13:55:54 -0500
7, 19 -- Received: from hrndva-web26-z01 ([10.128.132.117])
7, 19 -- by hrndva-smta02.mail.rr.com with ESMTP
7, 19 -- id {20090918185553697.GAMI4164-at-hrndva-smta02.mail.rr.com}
7, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 18:55:53 +0000
7, 19 -- Message-ID: {20090918185553.851KC.196039.root-at-hrndva-web26-z01}
7, 19 -- Date: Fri, 18 Sep 2009 18:55:53 +0000
7, 19 -- From: {joexray-at-cinci.rr.com}
7, 19 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 19 -- Subject: Cambridge S-90B SEM
7, 19 -- MIME-Version: 1.0
7, 19 -- Content-Type: text/plain; charset=utf-8
7, 19 -- Content-Transfer-Encoding: 7bit
7, 19 -- X-Priority: 3 (Normal)
7, 19 -- Sensitivity: Normal
7, 19 -- X-Originating-IP:
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Fri, 18 Sep 2009 16:03:48 -0500
Subject: [Microscopy] 2160 lines/mm Mag Cal Sample Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Which uncertainty do you mean? The reproducibility I saw with at TEM
sample rod at best with a centered and properly set eccentric height was
usually 2-3% without moving the translation knobs or removing and resetting
the grid in the holder. This was determined buy repeated removals and
insertions of the sample rod. Later, I tried spinning the eccentric knob
just before insertion and resetting the height. The value seemed to be
more like 3% unless I was very careful.

I think my factory specification on mags were stated to be within 5% of the
true magnification value.

Even if you have a perfectly flat and traceable standard, setting the
eccentric point and the reinsertion of the sample rod will introduce a few
percent of uncertainty.

For really critical work, I would take a carbon grating replica (CGR)
photograph, run my anti-reflective layer stack cross-section samples, and
then take another set of CGR photographs. So I sandwiched my measurements
between standard CGR image photographs. I could usually get as good as 0
to 2% variation versus the wider stated factory 5% value. So this method
included the removal and reinsertion of the same CGR. The grating images
were taken at the center of the grid and along the axis of the sample rod
for eccentric height reasons. If the CGR values agreed fairly well, I then
corrected the thickness of the layer. It was that critical to some of my
customers.

What "we" as a group are implying is that for almost 50 years or more,
microscopists have been using a CGR that was not the proper value because
it's was not certified as traceable by a document. I saw a diffraction
grating being made at Jerroll Ash in about 1975 or 1976 which was located
west of Boston. They used lasers to maintain a straight line groove,
simultaneously monitored the previous cut groove, and checked that the
spacing between the previously cut line and this current cut grating line
was correct. A video monitor from a microscope showed the laser beams
striking the grooves and the actual cutting of the surface. I was
impressed and it was fascinating to watch the process. I think "we" know
the spacing is as accurate as we need.

After almost 50 years, nobody has stepped forward and said the spacing is
way off on the cheaper and commonly used CGRs. I wonder why NIST, the old
NBS, never certified these grating rulings are accurate or wrong. Would it
be that after 50 years the CGR spacing is correct?

There are other standards one can buy and those were discussed by Ron
Anderson and I in our companion articles in May, 2009 of Microscopy Today.
Take your pick. I mean pick your dollar value and your vendor.

Even with a perfectly flat grid, flat grating ruling, and a perfect carbon
film replica; other errors will creep in, IMO. With careful techniques,
the deviation from the CGR spacing or other standard can be held to 2-3% on
a modern microscope that is maintained under factory service and aged less
than about 25 years. Even if you use a traceable standard, you still have
to change to the sample grid and exchange the rod.

JMO,

Paul

At 08:59 AM 9/18/09 -0500, colijn.1-at-osu.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
12, 26 -- From beaurega-at-westol.com Fri Sep 18 16:03:48 2009
12, 26 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IL3lxX014190
12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 16:03:47 -0500
12, 26 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
12, 26 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n8IL2ZrU007047
12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 17:02:37 -0400
12, 26 -- Received: (qmail 7746 invoked by uid 89); 18 Sep 2009 21:02:27 -0000
12, 26 -- Received: from pitts-69-72-13-9.dynamic-dialup.coretel.net (HELO running) (69.72.13.9)
12, 26 -- by mail.winbeam.com with SMTP; 18 Sep 2009 21:02:27 -0000
12, 26 -- Message-Id: {3.0.6.32.20090918170250.007d5860-at-pop3.norton.antivirus}
12, 26 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified)
12, 26 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
12, 26 -- Date: Fri, 18 Sep 2009 17:02:50 -0400
12, 26 -- To: microscopy-at-microscopy.com
12, 26 -- From: Beaurega {beaurega-at-westol.com}
12, 26 -- Subject: Re: [Microscopy] Re: 2160 lines/mm Mag Cal Sample Needed
12, 26 -- In-Reply-To: {200909181359.n8IDxpqK014479-at-ns.microscopy.com}
12, 26 -- Mime-Version: 1.0
12, 26 -- Content-Type: text/plain; charset="us-ascii"
12, 26 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
12, 26 -- X-Winbeam-MailScanner-ID: n8IL2ZrU007047
12, 26 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
12, 26 -- X-Winbeam-MailScanner-SpamCheck: not spam, SpamAssassin (not cached,
12, 26 -- score=0.406, required 4, AWL -1.41, SARE_SPEC_REPLICA_OBFU 1.81)
12, 26 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
==============================End of - Headers==============================




From: swtkeller-at-yahoo.com
Date: Fri, 18 Sep 2009 17:45:46 -0500
Subject: [Microscopy] viaWWW: TEM-looking for a double-tilt holder to fit Philips 420

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both swtkeller-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: swtkeller-at-yahoo.com
Name: Sandra Keller

Organization: TAS-SICCO

Title-Subject: [Filtered] TEM-looking for a double-tilt holder to fit
Philips 420 TEM

Question: Hi All:
I am looking for a used double-tilt holder for sale or trade that
will fit a Philips 420 TEM. We are looking to buy or trade. We have
several holders that will fit JEOL 100/200/1200 TEM's which we would
be willing to trade for.
Regards,
Sandra

Login Host: 71.91.112.63
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Sep 18 17:45:46 2009
6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IMji0k000911
6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 17:45:45 -0500
6, 12 -- Mime-Version: 1.0
6, 12 -- Message-Id: {p06240806c6d9be012d3b-at-[206.69.208.22]}
6, 12 -- Date: Fri, 18 Sep 2009 17:45:44 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: swtkeller-at-yahoo.com (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: TEM-looking for a double-tilt holder to fit Philips 420
6, 12 -- TEM
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: albinab-at-ornl.gov
Date: Mon, 21 Sep 2009 09:52:30 -0500
Subject: [Microscopy] Postdoctoral position available in High-Resolution Scanning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pharries-at-pittstate.edu as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pharries-at-pittstate.edu
Name: Phillip Harries

Organization: Pittsburg State University

Title-Subject: [Filtered] Used Confocal?

Question: My department is interested in purchasing a second-hand
confocal scope. We require a fairly basic setup capable of imaging
GFP/RFP and possibly CFP. If anyone has any suggestions as to where
we could find such a system it would be very much appreciated!

Login Host: 198.49.81.49
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Sep 18 17:46:17 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8IMkG7P001710
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2009 17:46:17 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240807c6d9be2134a2-at-[206.69.208.22]}
6, 11 -- Date: Fri, 18 Sep 2009 17:46:15 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: pharries-at-pittstate.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Used Confocal?
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From ibazan001-at-student.franciscan.edu Sun Sep 20 19:41:38 2009
Return-Path: {ibazan001-at-student.franciscan.edu}
Received: from studentgw.franciscan.edu (studentgw.franciscan.edu [206.244.99.249])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8L0fcYS011518
for {MicroscopyListserverArchive-at-microscopy.com} ; Sun, 20 Sep 2009 19:41:38 -0500
Received: from student-MTA by studentgw.franciscan.edu
with Novell_GroupWise; Sun, 20 Sep 2009 18:56:19 -0400
Message-Id: {sab67ad3.059-at-studentgw.franciscan.edu}
X-Mailer: Novell GroupWise Internet Agent 6.5.7

Dear listers,
Below please find a description of a postdoctoral opportunity at Oak
Ridge National Laboratory. Feel free to post or forward to any qualified
candidates. The position is available now.


*Postdoctoral Research Associate in High-Resolution Scanning
Transmission Electron Microscopy and Spectroscopy of Oxide Interfaces
and Nanowires*
*Materials Science and Technology Division*
*Oak Ridge National Laboratory *
*Oak Ridge, Tennessee*
*ORNL09-126-MSTD*
*Project Description:*
The Materials Science and Technology Division at Oak Ridge National
Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position
in the field of high-resolution scanning transmission electron
microscopy (STEM) and electron energy loss spectroscopy of oxide
interfaces and nanowires. This program takes advantage of ORNL's suite
of advanced electron microscopes, including five aberration-corrected
instruments.
This position provides an opportunity to join an experienced team
working in a highly collaborative environment on projects ranging from
heterogeneous catalysis to oxide materials to semiconductors.
Interactions with theoretical researchers are anticipated on a daily basis.
*Qualifications:*
This position requires a Ph.D. in Materials Science, Physics, or related
field, with an emphasis on advanced TEM or STEM. Knowledge of oxide
crystal chemistry is a plus. Excellent oral and written communication
skills are required, and presentations and publication of scientific
results in peer-reviewed journals are expected. The applicant must have
the ability to work in a team and interact effectively with a broad
range of colleagues. Applicants cannot have received the most recent
degree more than five years prior to the date of application and must
complete all degree requirements before starting their appointment.
The successful applicant must demonstrate experience in electron
microscope operation, preferably aberration-corrected microscopes, as
well as skills in analysis and interpretation of microscopic and
spectroscopic data.
Applicants cannot have received the most recent degree more than five
years prior to the date of application appointment and must complete all
degree requirements before starting their appointment.
*Technical Questions:*
Questions regarding the position can be directed to Drs. Albina Y.
Borisevich, albinab-at-ornl.gov {mailto:albinab-at-ornl.gov} , Stephen J.
Pennycook, pennycooksj-at-ornl.gov {mailto:pennycooksj-at-ornl.gov} , and
Sergei V. Kalinin, sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} .
*How to Apply:*
Qualified applicants may apply online at
https://www2.orau.gov/ORNL_POST/. All applicants will need to register
before they can begin the online application. For complete instructions,
on how to apply, please see the instructions at
http://www.orau.gov/orise/edu/ornl/orni-pdpm/application.htm. When
applying for this position, please reference the position title and number.
This appointment is offered through the ORNL Postgraduate Research
Participation Program and is administered by the Oak Ridge Institute for
Science and Education (ORISE). The program is open to all qualified U.S.
and non-U.S. citizens without regard to race, color, age, religion, sex,
national origin, physical or mental disability, or status as a
Vietnam-era veteran or disabled veteran.
--
--
Albina Y. Borisevich
R&D Associate
Electron Microscopy Group
Oak Ridge National Laboratory
Materials Science and Technology Division
PO Box 2008
Oak Ridge TN 37831-6031

http://stem.ornl.gov/

For express mail add: 1 Bethel Valley Road
phone: (865) 576-4060
fax: (865) 574-4143

==============================Original Headers==============================
5, 26 -- From albinab-at-ornl.gov Mon Sep 21 09:52:30 2009
5, 26 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LEqTrI001238
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 09:52:30 -0500
5, 26 -- Received: from emroute3.ornl.gov ([127.0.0.1])
5, 26 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
5, 26 -- with ESMTP id {0KQB00MK8TBG4C-at-emroute3.ornl.gov} for
5, 26 -- microscopy-at-microscopy.com; Mon, 21 Sep 2009 10:52:28 -0400 (EDT)
5, 26 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov
5, 26 -- (PMDF V6.4 #31561) id {0KQB00001TBF2X-at-emroute3.ornl.gov} for
5, 26 -- microscopy-at-microscopy.com; Mon, 21 Sep 2009 10:52:27 -0400 (EDT)
5, 26 -- Received: from [160.91.157.36] (simulations1.ornl.gov [160.91.157.36])
5, 26 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
5, 26 -- with ESMTPSA id {0KQB00MD8TBFHE-at-emroute3.ornl.gov} for
5, 26 -- microscopy-at-microscopy.com; Mon, 21 Sep 2009 10:52:27 -0400 (EDT)
5, 26 -- Date: Mon, 21 Sep 2009 10:52:27 -0400
5, 26 -- From: Albina Borisevich {albinab-at-ornl.gov}
5, 26 -- Subject: Postdoctoral position available in High-Resolution Scanning
5, 26 -- Transmission Electron Microscopy and Spectroscopy of Oxide Interfaces and
5, 26 -- Nanowires
5, 26 -- To: microscopy-at-microscopy.com
5, 26 -- Message-id: {4AB7932B.60003-at-ornl.gov}
5, 26 -- MIME-version: 1.0
5, 26 -- Content-type: text/plain; format=flowed; charset=UTF-8
5, 26 -- Content-transfer-encoding: 7bit
5, 26 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
==============================End of - Headers==============================




From: gul417-at-mail.usask.ca
Date: Mon, 21 Sep 2009 10:57:42 -0500
Subject: [Microscopy] Philips CM10 TEM: image with strange rectangular layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Lister:

Our Philip CM10 TEM occasionally produces double- or multiple-layered
images (exposures) in the plate films after development (see an example
at _http://www.usask.ca/biology/scopes/bad%20TEM%20image.pdf)_. It seems
that the “blocking object" is moving during exposure, resulted in one or
a few “rectangular planes†shifting spirally and being imposed on the
main image. It was not noticed on the main screen by eyes, and appeared
to occur in any magnifications.

It seems not due to development error, and nothing could be found in the
space between view screen and film plate, either.

In the same batch of the developed films, sometimes a few has this
phenomenon, sometimes most of them have it. Is it possible that was
caused by vibration during exposure?

Any advice will be greatly appreciated. Thanks you.

Guosheng



==============================Original Headers==============================
8, 21 -- From gul417-at-mail.usask.ca Mon Sep 21 10:57:42 2009
8, 21 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LFvfMf022397
8, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 10:57:42 -0500
8, 21 -- Received: from conversion-daemon.usask.ca by usask.ca
8, 21 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
8, 21 -- id {0KQB00601W0P52-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
8, 21 -- for Microscopy-at-microscopy.com; Mon, 21 Sep 2009 09:57:41 -0600 (CST)
8, 21 -- Received: from paws4.usask.ca (paws4.usask.ca [128.233.194.33])
8, 21 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
8, 21 -- with SMTP id {0KQB00ETJWC4UH-at-usask.ca} for Microscopy-at-microscopy.com; Mon,
8, 21 -- 21 Sep 2009 09:57:41 -0600 (CST)
8, 21 -- Date: Mon, 21 Sep 2009 09:57:40 -0600 (CST)
8, 21 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
8, 21 -- Subject: Philips CM10 TEM: image with strange rectangular layers
8, 21 -- To: Microscopy-at-microscopy.com
8, 21 -- Message-id: {19871235.1253548660816.JavaMail.gul417-at-mail.usask.ca}
8, 21 -- MIME-version: 1.0
8, 21 -- Content-type: text/plain; charset=UTF-8
8, 21 -- Content-Transfer-Encoding: 8bit
8, 21 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n8LFvfMf022397
==============================End of - Headers==============================




From: kmoulton-at-usm.maine.edu
Date: Mon, 21 Sep 2009 15:12:43 -0500
Subject: [Microscopy] Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have heard that one can use HMDS to dry samples for SEM - instead of
using a CPD. Does anyone have experience/orotocols they'd be willing
to share, for using this chemical?

Karen D. Moulton
Laboratory Manager
University of Southern Maine
Applied Medical Sciences
96 Falmouth Street
Portland, ME 04103




==============================Original Headers==============================
5, 26 -- From kmoulton-at-usm.maine.edu Mon Sep 21 15:12:43 2009
5, 26 -- Received: from mail0.doit.usm.maine.edu (mail0.doit.usm.maine.edu [130.111.135.148])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LKCh2V020875
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 15:12:43 -0500
5, 26 -- Received: from mail.usm.maine.edu (mail.usm.maine.edu [130.111.135.58])
5, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
5, 26 -- (No client certificate requested)
5, 26 -- by mail0.doit.usm.maine.edu (Postfix) with ESMTP id 7CB2C68E8CD
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 16:12:40 -0400 (EDT)
5, 26 -- Received: from moulton.ams.usm.maine.edu ([130.111.126.59])
5, 26 -- by mail.usm.maine.edu with ESMTP (TLS encrypted); Mon, 21 Sep 2009 16:12:27 -0400
5, 26 -- Message-Id: {8E4AE136-733F-4C23-832A-B07AD73FD47F-at-usm.maine.edu}
5, 26 -- From: Karen Moulton {kmoulton-at-usm.maine.edu}
5, 26 -- To: microscopy-at-microscopy.com
5, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 26 -- Content-Transfer-Encoding: 7bit
5, 26 -- Subject: Hexamethyldisilazane
5, 26 -- Mime-Version: 1.0 (Apple Message framework v936)
5, 26 -- Date: Mon, 21 Sep 2009 16:12:27 -0400
5, 26 -- X-Mailer: Apple Mail (2.936)
5, 26 -- X-USM-MailScanner-Information: Please contact USM for more information
5, 26 -- X-USM-MailScanner-ID: 7CB2C68E8CD.77EC1
5, 26 -- X-USM-MailScanner: Found to be clean
5, 26 -- X-USM-MailScanner-From: kmoulton-at-usm.maine.edu
5, 26 -- X-USM-MailScanner-Watermark: 1254168762.80152-at-sFrhwl6F0XQs4/IP58Sr5A
5, 26 -- X-Spam-Status: No
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 21 Sep 2009 15:27:03 -0500
Subject: [Microscopy] Re: Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Karen,

There are several methods in the literature. The protocols mostly
depend on what you're doing -- what do you want to do?
The general comment is "Use in a fume hood!"

Phil

} I have heard that one can use HMDS to dry samples for SEM - instead of
} using a CPD. Does anyone have experience/orotocols they'd be willing
} to share, for using this chemical?
}
} Karen D. Moulton
} Laboratory Manager
} University of Southern Maine
} Applied Medical Sciences
} 96 Falmouth Street
} Portland, ME 04103
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 25 -- From oshel1pe-at-cmich.edu Mon Sep 21 15:27:02 2009
4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LKR2VD002968
4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 15:27:02 -0500
4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id n8LKR2Su026359
4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 16:27:02 -0400
4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Mon, 21 Sep 2009 16:27:01 -0400
4, 25 -- Mime-Version: 1.0
4, 25 -- Message-Id: {f06240814c6dd90b9b22a-at-[141.209.160.249]}
4, 25 -- In-Reply-To: {200909212017.n8LKH5l5026494-at-ns.microscopy.com}
4, 25 -- References: {200909212017.n8LKH5l5026494-at-ns.microscopy.com}
4, 25 -- Date: Mon, 21 Sep 2009 16:26:58 -0400
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 25 -- Subject: Re: [Microscopy] Hexamethyldisilazane
4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 25 -- X-OriginalArrivalTime: 21 Sep 2009 20:27:01.0973 (UTC) FILETIME=[E0691050:01CA3AF9]
4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 25 -- X-Spam-Score: -4.20 () [Tag at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
4, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 25 -- X-CanItPRO-Stream: default
4, 25 -- X-Canit-Stats-ID: 18957299 - 1b2bddf65346 - 20090921
4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Mon, 21 Sep 2009 15:34:57 -0500
Subject: [Microscopy] Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lots of experience- Processes as usual for EM and for final drying transfer out of 100% alcohol step to 100% HMDS, 2-3 changes same time as your alcohol steps and then let air dry ( work in hood- not pleasant stuff). Slower drying seems better on more delicate samples so most of the time I wet a filter paper with HMDS in a Petri, dump on the wet sample and partially cover to allow slower evaporation. Most animal, cellular, and insect/invertebrate tissues do well, hit or miss with plant and fungal tissues with no apparent predictability as to what will/won't work. I have had problems in the past with samples sticking in plastic vials/tubes so do this in glass or dump into Petri with filter paper.

Like all EM smaller is better.

I don't have an actual reference handy, just been using it for a long time. You may get protocols that specify alcohol/HMDS dilution series, but I have not found it necessary.

Sincerely,


Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891


-----Original Message-----
X-from: kmoulton-at-usm.maine.edu [mailto:kmoulton-at-usm.maine.edu]
Sent: Monday, September 21, 2009 4:14 PM
To: Whittaker, Scott

I have heard that one can use HMDS to dry samples for SEM - instead of
using a CPD. Does anyone have experience/orotocols they'd be willing
to share, for using this chemical?

Karen D. Moulton
Laboratory Manager
University of Southern Maine
Applied Medical Sciences
96 Falmouth Street
Portland, ME 04103




==============================Original Headers==============================
5, 26 -- From kmoulton-at-usm.maine.edu Mon Sep 21 15:12:43 2009
5, 26 -- Received: from mail0.doit.usm.maine.edu (mail0.doit.usm.maine.edu [130.111.135.148])
5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LKCh2V020875
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 15:12:43 -0500
5, 26 -- Received: from mail.usm.maine.edu (mail.usm.maine.edu [130.111.135.58])
5, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
5, 26 -- (No client certificate requested)
5, 26 -- by mail0.doit.usm.maine.edu (Postfix) with ESMTP id 7CB2C68E8CD
5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 16:12:40 -0400 (EDT)
5, 26 -- Received: from moulton.ams.usm.maine.edu ([130.111.126.59])
5, 26 -- by mail.usm.maine.edu with ESMTP (TLS encrypted); Mon, 21 Sep 2009 16:12:27 -0400
5, 26 -- Message-Id: {8E4AE136-733F-4C23-832A-B07AD73FD47F-at-usm.maine.edu}
5, 26 -- From: Karen Moulton {kmoulton-at-usm.maine.edu}
5, 26 -- To: microscopy-at-microscopy.com
5, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
5, 26 -- Content-Transfer-Encoding: 7bit
5, 26 -- Subject: Hexamethyldisilazane
5, 26 -- Mime-Version: 1.0 (Apple Message framework v936)
5, 26 -- Date: Mon, 21 Sep 2009 16:12:27 -0400
5, 26 -- X-Mailer: Apple Mail (2.936)
5, 26 -- X-USM-MailScanner-Information: Please contact USM for more information
5, 26 -- X-USM-MailScanner-ID: 7CB2C68E8CD.77EC1
5, 26 -- X-USM-MailScanner: Found to be clean
5, 26 -- X-USM-MailScanner-From: kmoulton-at-usm.maine.edu
5, 26 -- X-USM-MailScanner-Watermark: 1254168762.80152-at-sFrhwl6F0XQs4/IP58Sr5A
5, 26 -- X-Spam-Status: No
==============================End of - Headers==============================


==============================Original Headers==============================
18, 29 -- From WHITTAKS-at-si.edu Mon Sep 21 15:34:57 2009
18, 29 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178])
18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LKYuBW017313
18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 15:34:57 -0500
18, 29 -- Received: from SI-MSEHUB-N02.US.SINET.SI.EDU (si-msehub-n02.us.sinet.si.edu [160.111.49.126])
18, 29 -- by si-mailout04.si.edu (Postfix) with ESMTP id 0BE547DEC;
18, 29 -- Mon, 21 Sep 2009 16:30:40 -0400 (EDT)
18, 29 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::58ac:1cff:44b0:524e]) by
18, 29 -- SI-MSEHUB-N02.US.SINET.SI.EDU ([fe80::dfb:a2f1:b0f7:9c85%11]) with mapi; Mon,
18, 29 -- 21 Sep 2009 16:34:56 -0400
18, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
18, 29 -- To: "'kmoulton-at-usm.maine.edu'" {kmoulton-at-usm.maine.edu}
18, 29 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
18, 29 -- Date: Mon, 21 Sep 2009 16:34:55 -0400
18, 29 -- Subject: RE: [Microscopy] Hexamethyldisilazane
18, 29 -- Thread-Topic: [Microscopy] Hexamethyldisilazane
18, 29 -- Thread-Index: Aco6+CAJoSnsK/bNSBmnQlrD4DO3nQAAOIxA
18, 29 -- Message-ID: {038C04650A93A14381E944A473695F618C6B07446B-at-SI-MSEV03.US.SINET.SI.EDU}
18, 29 -- References: {200909212014.n8LKERK8023038-at-ns.microscopy.com}
18, 29 -- In-Reply-To: {200909212014.n8LKERK8023038-at-ns.microscopy.com}
18, 29 -- Accept-Language: en-US
18, 29 -- Content-Language: en-US
18, 29 -- X-MS-Has-Attach:
18, 29 -- X-MS-TNEF-Correlator:
18, 29 -- acceptlanguage: en-US
18, 29 -- Content-Type: text/plain; charset="iso-8859-1"
18, 29 -- MIME-Version: 1.0
18, 29 -- Content-Transfer-Encoding: 8bit
18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8LKYuBW017313
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Mon, 21 Sep 2009 17:51:56 -0500
Subject: [Microscopy] viaWWW: OFFER: 2.3mm TEM Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I wish I had seen that online at your website but my searches didn't
bring that up. Now that I searched with the part number I see it's
listed under the TEM&STEM area which I wouldn't have looked under
since I have SEM/FIB.

So EMS does indeed carry the correct sample I was looking for at the
lower price the one I saw was for certified sample at the higher
price.

Sorry for all the confusion and thanks for clarifying.

---------- Forwarded message ----------
X-from: Stacie Kirsch {stacie-at-ems-secure.com}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] OFFER: 2.3mm TEM Grids

Question: I have a lot (around 50 vials) of all types of 2.3mm grids.
These are the kind that I believe were used in the VG STEM
microscopes. If anyone needs grids this size, I would be happy send
them to you.

Margaret E. Bisher

Electron Microscopy & Histology Core Facility Manager
Princeton University
Department of Molecular Biology
Moffett Laboratory, Room 113B
Washington Road
Princeton, New Jersey 08544
Voice: (609) 258-7026
Fax: (609) 258-8468
Email: mbisher-at-princeton.edu


Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Sep 21 17:51:56 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LMpskQ023368
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 17:51:55 -0500
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c6ddb3f6d735-at-[206.69.208.22]}
9, 11 -- Date: Mon, 21 Sep 2009 17:51:53 -0500
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: OFFER: 2.3mm TEM Grids
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: joanne.etheridge-at-mcem.monash.edu.au
Date: Mon, 21 Sep 2009 21:19:13 -0500
Subject: [Microscopy] viaWWW: Electron Microscopist & Research Fellow Positions Monash

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both
joanne.etheridge-at-mcem.monash.edu.au as well as
the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: joanne.etheridge-at-mcem.monash.edu.au
Name: Joanne Etheridge

Organization: Monash University

Title-Subject: [Filtered] Electron Microscopist & Research Fellow Positions

Question: The Monash Centre for Electron
Microscopy (MCEM) at Monash University in
Melbourne, Australia, is seeking outstanding
candidates for several new positions in electron
microscopy. The MCEM conducts research and
provides advanced instrumentation, training and
expertise in electron microscopy and atom probe
microscopy. The Centre has a world-class suite of
instrumentation housed in a dedicated, high
stability building designed to optimise
instrument performance.

Research Fellow ñ 3 Year appointment (Job Ref No: A0910254)

This position will conduct advanced research in
aberration-corrected TEM, STEM and related
techniques and applications. The successful
applicant will have high-level understanding and
skills in the theory and application of TEM and a
PhD or equivalent qualification.

Electron Microscopists (Three positions) -
Continuing appointments (Job Ref No.s: P0910255,
P0910256 and P0910257)

These positions provide expertise and training in
advanced electron microscopy to support the
research activities of researchers using the
Centre. The successful applicants will have
advanced understanding and skills in the
acquisition and interpretation of data from SEMs
and/or
TEMs and its application to the solution of problems in the physical sciences.
----------------------

For Position Descriptions and application
details, please go to: www.monash.edu/jobs and
type in the search box the keyword ìelectronî or
the specific job Ref. No.

For further information about these positions,
please contact A/Prof. Joanne Etheridge, Tel: +61
3 9905 1836 or email:
joanne.etheridge-at-mcem.monash.edu.au

For further information about the Monash Centre
for Electron Microscopy, please go to:
www.mcem.monash.edu.au.

CLOSING DATE: 16 October 2009


Login Host: 130.194.141.51
---------------------------------------------------------------------------


==============================Original Headers==============================
16, 14 -- From zaluzec-at-microscopy.com Mon Sep 21 21:19:12 2009
16, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
16, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8M2JB79011488
16, 14 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 21:19:12 -0500
16, 14 -- Mime-Version: 1.0
16, 14 -- Message-Id: {p06240800c6dde4759942-at-[206.69.208.22]}
16, 14 -- Date: Mon, 21 Sep 2009 21:19:10 -0500
16, 14 -- To: microscopy-at-microscopy.com
16, 14 -- From: joanne.etheridge-at-mcem.monash.edu.au (by way of MicroscopyListserver)
16, 14 -- Subject: viaWWW: Electron Microscopist & Research Fellow Positions Monash
16, 14 -- University
16, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
16, 14 -- Content-Transfer-Encoding: 8bit
16, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8M2JB79011488
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Tue, 22 Sep 2009 07:00:36 -0500
Subject: [Microscopy] Re: Philips CM10 TEM: image with strange rectangular layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I am not familiar with the type of camera shutter used on the CM10 but this
looks like a delay in shutter movement to me. Check the shutter movement as
your first guess?

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com

----- Original Message -----
X-from: {gul417-at-mail.usask.ca}
To: {protrain-at-emcourses.com}
Sent: Monday, September 21, 2009 4:58 PM

Hello,
This is really strange. The edges of “a shadow†are quite sharp.
It seems to me that it could be an effect of pre- or post- exposure of the plan
films emulsion during their putting in microscope cassette or removing them
from it in the dark room. A long time ago, we had some problems with pre- or
post- exposure of plan films after an exchange of red safety light bulb in our
dark-room with a new one. The red filter of the new one was not so safe as
should be.

Best regards from Prague
Oldrich

On Monday 21 of September 2009 17:59:54 gul417-at-mail.usask.ca wrote:
} ---------------------------------------------------------------------------
} - The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
} Dear Lister:
}
} Our Philip CM10 TEM occasionally produces double- or multiple-layered
} images (exposures) in the plate films after development (see an example
} at _http://www.usask.ca/biology/scopes/bad%20TEM%20image.pdf)_. It seems
} that the “blocking object" is moving during exposure, resulted in one or
} a few “rectangular planes†shifting spirally and being imposed on the
} main image. It was not noticed on the main screen by eyes, and appeared
} to occur in any magnifications.
}
} It seems not due to development error, and nothing could be found in the
} space between view screen and film plate, either.
}
} In the same batch of the developed films, sometimes a few has this
} phenomenon, sometimes most of them have it. Is it possible that was
} caused by vibration during exposure?
}
} Any advice will be greatly appreciated. Thanks you.
}
} Guosheng


==============================Original Headers==============================
4, 23 -- From benada-at-biomed.cas.cz Tue Sep 22 07:00:35 2009
4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MC0ZSF032293
4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 07:00:35 -0500
4, 23 -- Received: from u117ob.localnet (c100br.mbu.cas.cz [147.231.44.1])
4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
4, 23 -- (No client certificate requested)
4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 55C561A445B1;
4, 23 -- Tue, 22 Sep 2009 14:00:02 +0200 (CEST)
4, 23 -- From: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz}
4, 23 -- Organization: =?utf-8?q?Mikrobiogick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i.
4, 23 -- To: gul417-at-mail.usask.ca, microscopy-at-microscopy.com
4, 23 -- Subject: Re: [Microscopy] Philips CM10 TEM: image with strange rectangular layers
4, 23 -- Date: Tue, 22 Sep 2009 14:00:24 +0200
4, 23 -- User-Agent: KMail/1.12.1 (Linux/2.6.30-1-686; KDE/4.3.1; i686; ; )
4, 23 -- References: {200909211559.n8LFxsWR026130-at-ns.microscopy.com}
4, 23 -- In-Reply-To: {200909211559.n8LFxsWR026130-at-ns.microscopy.com}
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: Text/Plain;
4, 23 -- charset="utf-8"
4, 23 -- Message-Id: {200909221400.24832.benada-at-biomed.cas.cz}
4, 23 -- Content-Transfer-Encoding: 8bit
4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8MC0ZSF032293
==============================End of - Headers==============================




From: renaudgeological-at-execulink.com
Date: Tue, 22 Sep 2009 08:26:40 -0500
Subject: [Microscopy] viaWWW: Objective lens oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both renaudgeological-at-execulink.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: renaudgeological-at-execulink.com
Name: Jim

Title-Subject: [Filtered] Objective lens oil

Question: Question: Hi all,

I am looking to top up the objective lens oil reservoir on my
electron microprobe. I used to be able to purchase "Voltesso 35" in
a 5 gallon pail but now can only purchase it in 45 gallon drums which
seems a little ridciulous. Just wondering if anyone can suggest an
alternative to "Voltesso 35" that can be purchased in a more
reasonable quantity. Thanks,

Jim.

Login Host: 209.239.5.56

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Sep 22 08:26:40 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MDQdvp016779
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 08:26:40 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240804c6de80dfbdcf-at-[206.69.208.22]}
8, 11 -- Date: Tue, 22 Sep 2009 08:26:39 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: renaudgeological-at-execulink.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Objective lens oil
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 22 Sep 2009 08:44:18 -0500
Subject: [Microscopy] Re: Philips CM10 TEM: image with strange rectangular layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with Oldrich. We had a similar problem and finally tracked it down to pinhole leaks in our safelight filter. Over the years, the red acetate (?) layer in our Kodak safelight had deteriorated until it actually formed tiny physical holes. At some point, quite suddenly, it reached the tipping point where a couple batches of films showed exactly this effect. We were quite puzzled, checking outside light leaks into our film development and loading cubicle and finding none, until we finally thought to check the safelight. Problem solved.

The particular batch of film that was affected was shot by none other than our very own Tom Phillips of Microscopy Board fame! He was not amused, but took it with relatively good humor, considering. We're still friends.

Please check your safelight. I would bet good money that your scope has nothing to do with this.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz]
Sent: Tuesday, September 22, 2009 7:02 AM
To: Tindall, Randy D.

Hello,
This is really strange. The edges of “a shadow†are quite sharp.
It seems to me that it could be an effect of pre- or post- exposure of the plan
films emulsion during their putting in microscope cassette or removing them
from it in the dark room. A long time ago, we had some problems with pre- or
post- exposure of plan films after an exchange of red safety light bulb in our
dark-room with a new one. The red filter of the new one was not so safe as
should be.

Best regards from Prague
Oldrich

On Monday 21 of September 2009 17:59:54 gul417-at-mail.usask.ca wrote:
} ---------------------------------------------------------------------------
} - The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
} Dear Lister:
}
} Our Philip CM10 TEM occasionally produces double- or multiple-layered
} images (exposures) in the plate films after development (see an example
} at _http://www.usask.ca/biology/scopes/bad%20TEM%20image.pdf)_. It seems
} that the “blocking object" is moving during exposure, resulted in one or
} a few “rectangular planes†shifting spirally and being imposed on the
} main image. It was not noticed on the main screen by eyes, and appeared
} to occur in any magnifications.
}
} It seems not due to development error, and nothing could be found in the
} space between view screen and film plate, either.
}
} In the same batch of the developed films, sometimes a few has this
} phenomenon, sometimes most of them have it. Is it possible that was
} caused by vibration during exposure?
}
} Any advice will be greatly appreciated. Thanks you.
}
} Guosheng


==============================Original Headers==============================
4, 23 -- From benada-at-biomed.cas.cz Tue Sep 22 07:00:35 2009
4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MC0ZSF032293
4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 07:00:35 -0500
4, 23 -- Received: from u117ob.localnet (c100br.mbu.cas.cz [147.231.44.1])
4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
4, 23 -- (No client certificate requested)
4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 55C561A445B1;
4, 23 -- Tue, 22 Sep 2009 14:00:02 +0200 (CEST)
4, 23 -- From: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz}
4, 23 -- Organization: =?utf-8?q?Mikrobiogick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i.
4, 23 -- To: gul417-at-mail.usask.ca, microscopy-at-microscopy.com
4, 23 -- Subject: Re: [Microscopy] Philips CM10 TEM: image with strange rectangular layers
4, 23 -- Date: Tue, 22 Sep 2009 14:00:24 +0200
4, 23 -- User-Agent: KMail/1.12.1 (Linux/2.6.30-1-686; KDE/4.3.1; i686; ; )
4, 23 -- References: {200909211559.n8LFxsWR026130-at-ns.microscopy.com}
4, 23 -- In-Reply-To: {200909211559.n8LFxsWR026130-at-ns.microscopy.com}
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: Text/Plain;
4, 23 -- charset="utf-8"
4, 23 -- Message-Id: {200909221400.24832.benada-at-biomed.cas.cz}
4, 23 -- Content-Transfer-Encoding: 8bit
4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8MC0ZSF032293
==============================End of - Headers==============================


==============================Original Headers==============================
17, 34 -- From TindallR-at-missouri.edu Tue Sep 22 08:44:18 2009
17, 34 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
17, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MDiHqJ031567
17, 34 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 08:44:18 -0500
17, 34 -- X-IronPort-Anti-Spam-Filtered: true
17, 34 -- X-IronPort-Anti-Spam-Result: ApsEAAZxuErRauUo/2dsb2JhbACZQ68IAQmGb4hKgkeBVAWBWA
17, 34 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
17, 34 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 22 Sep 2009 08:44:17 -0500
17, 34 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
17, 34 -- Tue, 22 Sep 2009 08:44:17 -0500
17, 34 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
17, 34 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Tue, 22 Sep 2009
17, 34 -- 08:44:15 -0500
17, 34 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
17, 34 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
17, 34 -- Date: Tue, 22 Sep 2009 08:44:15 -0500
17, 34 -- Subject: RE: [Microscopy] Re: Philips CM10 TEM: image with strange
17, 34 -- rectangular layers
17, 34 -- Thread-Topic: [Microscopy] Re: Philips CM10 TEM: image with strange
17, 34 -- rectangular layers
17, 34 -- Thread-Index: Aco7fHQ5tBRl5O+jRhyS2ieVMvs1qQADYEtQ
17, 34 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129877C903-at-UM-EMAIL06.um.umsystem.edu}
17, 34 -- References: {200909221201.n8MC1Vnw001384-at-ns.microscopy.com}
17, 34 -- In-Reply-To: {200909221201.n8MC1Vnw001384-at-ns.microscopy.com}
17, 34 -- Accept-Language: en-US
17, 34 -- Content-Language: en-US
17, 34 -- X-MS-Has-Attach:
17, 34 -- X-MS-TNEF-Correlator:
17, 34 -- acceptlanguage: en-US
17, 34 -- Content-Type: text/plain; charset="utf-8"
17, 34 -- MIME-Version: 1.0
17, 34 -- X-OriginalArrivalTime: 22 Sep 2009 13:44:17.0229 (UTC) FILETIME=[C7837FD0:01CA3B8A]
17, 34 -- Content-Transfer-Encoding: 8bit
17, 34 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n8MDiHqJ031567
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Tue, 22 Sep 2009 11:45:00 -0500
Subject: [Microscopy] Re: Philips CM10 TEM: image with strange

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I agree with Randy and Oldrich. This also happened to me and it was not
the microscope.

Here's what I would add. I tired to switch and use a low wattage CFL bulb
that unfortunately gave off more UV but had "extended bulb life". The CFL
ruined the safelight filter over time and caused this light leaking
problem. Heat buildup was still a problem. I switched back to tungsten
and bought three new safelight filters.

Things to consider:

*If they "outlaw" tungsten bulbs, you might want to stockpile a few cartons
of tungsten 15 watt bulbs in the USA but that's just a Band-Aid solution.

* Specially cut round *extra* UV absorbing "conservation glass" from an oil
painting framer might work. Will the coating still absorb 99% of the
emitted UV at that very short distance from a CFL bulb? Who knows.

*Indirect CFL lighting bounced off the ceiling might work but I never tried
that.

JMO,

Paul Beauregard

At 08:44 AM 9/22/09 -0500, TindallR-at-missouri.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
6, 28 -- From beaurega-at-westol.com Tue Sep 22 11:45:00 2009
6, 28 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16])
6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MGixLS023698
6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 11:45:00 -0500
6, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
6, 28 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id n8MGijEh010100
6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 12:44:52 -0400
6, 28 -- Received: (qmail 15452 invoked by uid 89); 22 Sep 2009 16:44:35 -0000
6, 28 -- Received: from pitts-69-72-22-96.dynamic-dialup.coretel.net (HELO running) (69.72.22.96)
6, 28 -- by mail.winbeam.com with SMTP; 22 Sep 2009 16:44:35 -0000
6, 28 -- Message-Id: {3.0.6.32.20090922124441.007d69d0-at-pop3.norton.antivirus}
6, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified)
6, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
6, 28 -- Date: Tue, 22 Sep 2009 12:44:41 -0400
6, 28 -- To: microscopy-at-microscopy.com
6, 28 -- From: Beaurega {beaurega-at-westol.com}
6, 28 -- Subject: Re: [Microscopy] Philips CM10 TEM: image with strange
6, 28 -- In-Reply-To: {200909221344.n8MDidqf032162-at-ns.microscopy.com}
6, 28 -- Mime-Version: 1.0
6, 28 -- Content-Type: text/plain; charset="iso-8859-1"
6, 28 -- Content-Transfer-Encoding: 8bit
6, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
6, 28 -- X-Winbeam-MailScanner-ID: n8MGijEh010100
6, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
6, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam, SpamAssassin (not cached,
6, 28 -- score=-1.243, required 4, autolearn=not spam, AWL 0.68,
6, 28 -- BAYES_00 -2.00, TW_MX 0.08)
6, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Tue, 22 Sep 2009 12:17:43 -0500
Subject: [Microscopy] Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Fume hood yes indeed.

I have a caution for anyone using HMDS: read the MSDS.

From the MSDS on the EMS website:
(http://www.emsdiasum.com/microscopy/technical/msds/16700.pdf)
CONDITIONS TO AVOID: Contact with water or alcohols generates
ammonia;

As we normally use it, especially if there is a graded series mixed with
ethanol, the HMDS does gets mixed with at least some ethanol and GLP for
waste management has us put the used mixture in a nicely sealed waste
bottle until pickup. I unscrewed the cap of a waste bottle with a
mixture of HMDS and ethanol to add more during a subsequent preparation
and it had great pressure, violently escaping gas (most likely ammonia)
and it bubbled violently for at least an hour. I am very lucky the
bottle didn't explode in my face.

So this is just a warning that our protocols dictate we use this
chemical in a way that is contrary to the MSDS advice; the product
generates gas and substantial pressure in a closed container.

Additionally, I wonder what that ammonia and raised pH may be doing to
structure.....

Dale

oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Karen,
}
} There are several methods in the literature. The protocols mostly
} depend on what you're doing -- what do you want to do?
} The general comment is "Use in a fume hood!"
}
} Phil
}
} } I have heard that one can use HMDS to dry samples for SEM - instead of
} } using a CPD. Does anyone have experience/orotocols they'd be willing
} } to share, for using this chemical?
} }
} } Karen D. Moulton
} } Laboratory Manager
} } University of Southern Maine
} } Applied Medical Sciences
} } 96 Falmouth Street
} } Portland, ME 04103


==============================Original Headers==============================
10, 22 -- From dac-at-research.umass.edu Tue Sep 22 12:17:43 2009
10, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MHHgKe006931
10, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 12:17:42 -0500
10, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
10, 22 -- (authenticated bits=0)
10, 22 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n8MHHgUT024148
10, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 13:17:42 -0400
10, 22 -- Message-ID: {4AB906C2.8080501-at-research.umass.edu}
10, 22 -- Date: Tue, 22 Sep 2009 13:17:54 -0400
10, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
10, 22 -- Reply-To: dac-at-research.umass.edu
10, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.23) Gecko/20090825 SeaMonkey/1.1.18
10, 22 -- MIME-Version: 1.0
10, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
10, 22 -- Subject: Re: [Microscopy] Re: Hexamethyldisilazane
10, 22 -- References: {200909212030.n8LKUabV010683-at-ns.microscopy.com}
10, 22 -- In-Reply-To: {200909212030.n8LKUabV010683-at-ns.microscopy.com}
10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 22 -- Content-Transfer-Encoding: 7bit
10, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 22 Sep 2009 13:29:45 -0500
Subject: [Microscopy] RE: Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: kmoulton-at-usm.maine.edu [mailto:kmoulton-at-usm.maine.edu]
} Sent: Monday, September 21, 2009 3:13 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Hexamethyldisilazane
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I have heard that one can use HMDS to dry samples for SEM -
} instead of using a CPD. Does anyone have
} experience/orotocols they'd be willing to share, for using
} this chemical?
}
} Karen D. Moulton
} Laboratory Manager
} University of Southern Maine
} Applied Medical Sciences
} 96 Falmouth Street
} Portland, ME 04103
}
}
}
}
} ==============================Original
} Headers==============================
} 5, 26 -- From kmoulton-at-usm.maine.edu Mon Sep 21 15:12:43 2009
} 5, 26 -- Received: from mail0.doit.usm.maine.edu
} (mail0.doit.usm.maine.edu [130.111.135.148])
} 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n8LKCh2V020875
} 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep
} 2009 15:12:43 -0500
} 5, 26 -- Received: from mail.usm.maine.edu
} (mail.usm.maine.edu [130.111.135.58])
} 5, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA
} (256/256 bits))
} 5, 26 -- (No client certificate requested)
} 5, 26 -- by mail0.doit.usm.maine.edu (Postfix) with
} ESMTP id 7CB2C68E8CD
} 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep
} 2009 16:12:40 -0400 (EDT)
} 5, 26 -- Received: from moulton.ams.usm.maine.edu ([130.111.126.59])
} 5, 26 -- by mail.usm.maine.edu with ESMTP (TLS
} encrypted); Mon, 21 Sep 2009 16:12:27 -0400
} 5, 26 -- Message-Id:
} {8E4AE136-733F-4C23-832A-B07AD73FD47F-at-usm.maine.edu}
} 5, 26 -- From: Karen Moulton {kmoulton-at-usm.maine.edu} 5, 26
} -- To: microscopy-at-microscopy.com 5, 26 -- Content-Type:
} text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 26
} -- Content-Transfer-Encoding: 7bit 5, 26 -- Subject:
} Hexamethyldisilazane 5, 26 -- Mime-Version: 1.0 (Apple
} Message framework v936) 5, 26 -- Date: Mon, 21 Sep 2009
} 16:12:27 -0400 5, 26 -- X-Mailer: Apple Mail (2.936) 5, 26 --
} X-USM-MailScanner-Information: Please contact USM for more
} information 5, 26 -- X-USM-MailScanner-ID: 7CB2C68E8CD.77EC1
} 5, 26 -- X-USM-MailScanner: Found to be clean 5, 26 --
} X-USM-MailScanner-From: kmoulton-at-usm.maine.edu 5, 26 --
} X-USM-MailScanner-Watermark: 1254168762.80152-at-sFrhwl6F0XQs4/IP58Sr5A
} 5, 26 -- X-Spam-Status: No
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Tue Sep 22 13:29:44 2009
7, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MITiZe024211
7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 13:29:44 -0500
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Tue, 22 Sep 2009 13:29:44 -0500
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] Hexamethyldisilazane
7, 25 -- Date: Tue, 22 Sep 2009 13:29:43 -0500
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB8EA-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200909212013.n8LKDLer021701-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] Hexamethyldisilazane
7, 25 -- thread-index: Aco69/g8CyIEBAvTTbqcjkS5qcBswAAuqHRg
7, 25 -- References: {200909212013.n8LKDLer021701-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {kmoulton-at-usm.maine.edu} , {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 22 Sep 2009 18:29:44.0246 (UTC) FILETIME=[A802E560:01CA3BB2]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8MITiZe024211
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Tue, 22 Sep 2009 13:43:19 -0500
Subject: [Microscopy] RE: Hexamethyldisilazane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am using HMDS for cell cultures (and some other specimens) with pretty
good results and simple protocol (everything done in a fume hood):

After removing of the last change of ethanol add HMDS. Keep in open
vessel. After 10 minutes pipette out most of HMDS (leave just enough to
cover specimen) and let it air dry.

If EDS should be performed, then treatment with HMDS is not the best
choice: it infuses specimens with silicone.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: kmoulton-at-usm.maine.edu [mailto:kmoulton-at-usm.maine.edu]
} Sent: Monday, September 21, 2009 3:13 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Hexamethyldisilazane
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I have heard that one can use HMDS to dry samples for SEM -
} instead of using a CPD. Does anyone have
} experience/orotocols they'd be willing to share, for using
} this chemical?
}
} Karen D. Moulton
} Laboratory Manager
} University of Southern Maine
} Applied Medical Sciences
} 96 Falmouth Street
} Portland, ME 04103
}
}
}
}
} ==============================Original
} Headers==============================
} 5, 26 -- From kmoulton-at-usm.maine.edu Mon Sep 21 15:12:43 2009
} 5, 26 -- Received: from mail0.doit.usm.maine.edu
} (mail0.doit.usm.maine.edu [130.111.135.148])
} 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id n8LKCh2V020875
} 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep
} 2009 15:12:43 -0500
} 5, 26 -- Received: from mail.usm.maine.edu
} (mail.usm.maine.edu [130.111.135.58])
} 5, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA
} (256/256 bits))
} 5, 26 -- (No client certificate requested)
} 5, 26 -- by mail0.doit.usm.maine.edu (Postfix) with
} ESMTP id 7CB2C68E8CD
} 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep
} 2009 16:12:40 -0400 (EDT)
} 5, 26 -- Received: from moulton.ams.usm.maine.edu ([130.111.126.59])
} 5, 26 -- by mail.usm.maine.edu with ESMTP (TLS
} encrypted); Mon, 21 Sep 2009 16:12:27 -0400
} 5, 26 -- Message-Id:
} {8E4AE136-733F-4C23-832A-B07AD73FD47F-at-usm.maine.edu}
} 5, 26 -- From: Karen Moulton {kmoulton-at-usm.maine.edu} 5, 26
} -- To: microscopy-at-microscopy.com 5, 26 -- Content-Type:
} text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 26
} -- Content-Transfer-Encoding: 7bit 5, 26 -- Subject:
} Hexamethyldisilazane 5, 26 -- Mime-Version: 1.0 (Apple
} Message framework v936) 5, 26 -- Date: Mon, 21 Sep 2009
} 16:12:27 -0400 5, 26 -- X-Mailer: Apple Mail (2.936) 5, 26 --
} X-USM-MailScanner-Information: Please contact USM for more
} information 5, 26 -- X-USM-MailScanner-ID: 7CB2C68E8CD.77EC1
} 5, 26 -- X-USM-MailScanner: Found to be clean 5, 26 --
} X-USM-MailScanner-From: kmoulton-at-usm.maine.edu 5, 26 --
} X-USM-MailScanner-Watermark: 1254168762.80152-at-sFrhwl6F0XQs4/IP58Sr5A
} 5, 26 -- X-Spam-Status: No
} ==============================End of -
} Headers==============================
}
}


==============================Original Headers==============================
9, 25 -- From DusevichV-at-umkc.edu Tue Sep 22 13:43:19 2009
9, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155])
9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MIhIlG006613
9, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 13:43:19 -0500
9, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 25 -- Tue, 22 Sep 2009 13:43:17 -0500
9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 25 -- Content-class: urn:content-classes:message
9, 25 -- MIME-Version: 1.0
9, 25 -- Content-Type: text/plain;
9, 25 -- charset="us-ascii"
9, 25 -- Subject: RE: [Microscopy] Hexamethyldisilazane
9, 25 -- Date: Tue, 22 Sep 2009 13:43:17 -0500
9, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB8EB-at-KC-MSX1.kc.umkc.edu}
9, 25 -- In-Reply-To: {200909212013.n8LKDLer021701-at-ns.microscopy.com}
9, 25 -- X-MS-Has-Attach:
9, 25 -- X-MS-TNEF-Correlator:
9, 25 -- Thread-Topic: [Microscopy] Hexamethyldisilazane
9, 25 -- thread-index: Aco69/g8CyIEBAvTTbqcjkS5qcBswAAvIyXw
9, 25 -- References: {200909212013.n8LKDLer021701-at-ns.microscopy.com}
9, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
9, 25 -- To: {kmoulton-at-usm.maine.edu} , {Microscopy-at-microscopy.com}
9, 25 -- X-OriginalArrivalTime: 22 Sep 2009 18:43:17.0462 (UTC) FILETIME=[8CB9CF60:01CA3BB4]
9, 25 -- Content-Transfer-Encoding: 8bit
9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8MIhIlG006613
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Tue, 22 Sep 2009 14:17:17 -0500
Subject: [Microscopy] Re: Philips CM10 TEM: image with strange rectangular

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Although the leaky safelight is a possible explanation, and I don't know
what kind of film transort system the CM10 has (in and out, or
circular),let me offer this story. I was having a similar problem with our
Zeiss 10/A, which has a circular film transport. I kept sending images to
our service manager and said I thought the transporter was moving during
exposure, to which he replied "Impossible!". (He had said this to me many
times over the years, but my microscopes are completely capable of
performing the "impossible".) It took over a year and many, many hours of
disassembly and reassembly of the camera transport components, but it
turns out that the 24V motor that ran the transport was stuttering on the
start, and it was all due to low incoming line voltage! Yes, we had
replaced the motor and capacitors and all else several times, but the
incoming voltage was just low enough to cause the problem. We now have
buck/boost on all three phases of our newer TEM. Low line voltage has also
caused problems with our water chillers.

Hope this helps!

Aloha,
Tina

} Our Philip CM10 TEM occasionally produces double- or multiple-layered
} images (exposures) in the plate films after development (see an example
} at _http://www.usask.ca/biology/scopes/bad%20TEM%20image.pdf)_. It seems
} that the “blocking object" is moving during exposure, resulted in one or
} a few “rectangular planes†shifting spirally and being imposed on the
} main image. It was not noticed on the main screen by eyes, and appeared
} to occur in any magnifications.
}
} It seems not due to development error, and nothing could be found in the
} space between view screen and film plate, either.
}
} In the same batch of the developed films, sometimes a few has this
} phenomenon, sometimes most of them have it. Is it possible that was
} caused by vibration during exposure?
}
} Any advice will be greatly appreciated. Thanks you.
}
} Guosheng
}
}
}
} ==============================Original Headers==============================
} 8, 21 -- From gul417-at-mail.usask.ca Mon Sep 21 10:57:42 2009
} 8, 21 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
} 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8LFvfMf022397
} 8, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Sep 2009 10:57:42 -0500
} 8, 21 -- Received: from conversion-daemon.usask.ca by usask.ca
} 8, 21 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
} 8, 21 -- id {0KQB00601W0P52-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
} 8, 21 -- for Microscopy-at-microscopy.com; Mon, 21 Sep 2009 09:57:41 -0600 (CST)
} 8, 21 -- Received: from paws4.usask.ca (paws4.usask.ca [128.233.194.33])
} 8, 21 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
} 8, 21 -- with SMTP id {0KQB00ETJWC4UH-at-usask.ca} for Microscopy-at-microscopy.com; Mon,
} 8, 21 -- 21 Sep 2009 09:57:41 -0600 (CST)
} 8, 21 -- Date: Mon, 21 Sep 2009 09:57:40 -0600 (CST)
} 8, 21 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
} 8, 21 -- Subject: Philips CM10 TEM: image with strange rectangular layers
} 8, 21 -- To: Microscopy-at-microscopy.com
} 8, 21 -- Message-id: {19871235.1253548660816.JavaMail.gul417-at-mail.usask.ca}
} 8, 21 -- MIME-version: 1.0
} 8, 21 -- Content-type: text/plain; charset=UTF-8
} 8, 21 -- Content-Transfer-Encoding: 8bit
} 8, 21 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n8LFvfMf022397
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




==============================Original Headers==============================
8, 24 -- From tina-at-pbrc.hawaii.edu Tue Sep 22 14:17:16 2009
8, 24 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MJHGKF022648
8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 14:17:16 -0500
8, 24 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 24 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n8MJH9VL021687
8, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
8, 24 -- Tue, 22 Sep 2009 09:17:10 -1000 (HST)
8, 24 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n8MJH8bc021684;
8, 24 -- Tue, 22 Sep 2009 09:17:08 -1000 (HST)
8, 24 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
8, 24 -- Date: Tue, 22 Sep 2009 09:17:07 -1000 (HST)
8, 24 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 24 -- X-Sender: tina-at-halia
8, 24 -- To: gul417-at-mail.usask.ca
8, 24 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 24 -- Subject: Re: [Microscopy] Philips CM10 TEM: image with strange rectangular
8, 24 -- layers
8, 24 -- In-Reply-To: {200909211558.n8LFwUTk023824-at-ns.microscopy.com}
8, 24 -- Message-ID: {Pine.GSO.4.21.0909220902470.21653-100000-at-halia}
8, 24 -- MIME-Version: 1.0
8, 24 -- Content-Type: TEXT/PLAIN; charset=X-UNKNOWN
8, 24 -- Content-Transfer-Encoding: 8bit
8, 24 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n8MJHGKF022648
==============================End of - Headers==============================




From: gul417-at-mail.usask.ca
Date: Tue, 22 Sep 2009 16:40:38 -0500
Subject: [Microscopy] Thank you all for the advice on TEM CM10 image problem--it was solved!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I appreciate very much for all the EM/imaging experts out there who've responded with excellent advice and comments in regard to our Philips CM10 TEM problem (image with strange rectangular layers).

As most of you pointed, the problem indeed came from the safe light in our dark room, more exactly from the "safe" light in the TEM room next door. After developing a bunch of films under different condition, I found that the red light (2 light bulbs, more than 16w I believe) in the TEM room are strong enough to expose the films during my loading/unloading. If the door for the dark room is closed, everything is OK. The reason I did not close the door sometimes was that a new telephone was installed and the cord was preventing the door from shutting tightly!!!

Thank you again!

Cheers,

Guosheng


==============================Original Headers==============================
7, 20 -- From gul417-at-mail.usask.ca Tue Sep 22 16:40:38 2009
7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MLebc0010188
7, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 16:40:38 -0500
7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca
7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- id {0KQE001016QCB0-at-usask.ca} (original mail from gul417-at-mail.usask.ca)
7, 20 -- for Microscopy-at-microscopy.com; Tue, 22 Sep 2009 15:40:36 -0600 (CST)
7, 20 -- Received: from paws2.usask.ca (paws2.usask.ca [128.233.194.30])
7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007))
7, 20 -- with SMTP id {0KQE00ILP6VN32-at-usask.ca} for Microscopy-at-microscopy.com; Tue,
7, 20 -- 22 Sep 2009 15:40:35 -0600 (CST)
7, 20 -- Date: Tue, 22 Sep 2009 15:40:34 -0600 (CST)
7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca}
7, 20 -- Subject: Thank you all for the advice on TEM CM10 image problem--it was solved!
7, 20 -- To: Microscopy-at-microscopy.com
7, 20 -- Message-id: {17493982.1253655634686.JavaMail.gul417-at-mail.usask.ca}
7, 20 -- MIME-version: 1.0
7, 20 -- Content-type: text/plain; charset=UTF-8
7, 20 -- Content-transfer-encoding: 7BIT
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 23 Sep 2009 06:37:46 -0500
Subject: [Microscopy] Re: viaWWW: Objective lens oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

3 points:

1) no, this is not the TEM shutter. Philips CM series shutter is not
mechanical - it is a coil that pulls the e-beam off optical axis of the
column when activated. No moving parts.

2) I don't think that the negative moved in the holder that much - that
would cause a jam.

3) Please notice appearance of the shadow. The corner and the top edge (that
is in fact the right edge) are sharp, and the adjacent edge is fuzzy, more
so away from the corner. A conductive object in the electron beam from a
'point' source would cast shadow with all edges sharp, even if the object
was located at a distance from the film and was not parallel to the film.
Just like a shadow of the diffraction beam stop. But a shadow from an
object in the light of a nearby (large) source has fuzzy edges. Object
casting that shadow was not parallel to negative but rather tilted, with one
side perhaps touching the film.

I suspect ambient light. Are you sure about integrity of the dark room and
cassettes? Is loading/unloading of the film done correctly? What about
ambient light in the TEM room? Any computer screens? Cell phone display?
Flashlight? Anything else?

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

----- Original Message -----
X-from: {protrain-at-emcourses.com}
To: {vitalylazar-at-att.net}
Sent: Tuesday, September 22, 2009 6:35 AM

try Diala AX transformer oil - see
http://www.mil-specproducts.com/productPage.aspx?prodID=518 . A 5 gallon can
costs some $55. Diala is a Shell product. I used it many times for HT tanks
repairs. Order on-line or call Shell rep. and ask for distributor in your
area. They are everywhere - all power utility companies must service their
transformers.

Castrol equivalent is Brayco 730. Didn't try it but should be the same.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com


----- Original Message -----
X-from: {renaudgeological-at-execulink.com}
To: {vitalylazar-at-att.net}
Sent: Tuesday, September 22, 2009 9:27 AM

Ask your local electricity board. They can probably give you some for
free. I tried here twice for a X-ray generator HV tank, and I get each
time 5 -10 liter for free. Our lab needs are dropplets in comparison
with what they use !

By the way, as I never worked on a microprobe, I don't understand where
you have to put this oil. You speak from an OL oil reservoir. Is the
coil of the Ol oil cooled ? Or is something there at HV ? Thanks for
some clarifications !

Jacques

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



renaudgeological-at-execulink.com a écrit :
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both renaudgeological-at-execulink.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: renaudgeological-at-execulink.com
} Name: Jim
}
} Title-Subject: [Filtered] Objective lens oil
}
} Question: Question: Hi all,
}
} I am looking to top up the objective lens oil reservoir on my
} electron microprobe. I used to be able to purchase "Voltesso 35" in
} a 5 gallon pail but now can only purchase it in 45 gallon drums which
} seems a little ridciulous. Just wondering if anyone can suggest an
} alternative to "Voltesso 35" that can be purchased in a more
} reasonable quantity. Thanks,
}
} Jim.
}
} Login Host: 209.239.5.56
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Tue Sep 22 08:26:40 2009
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8MDQdvp016779
} 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2009 08:26:40 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240804c6de80dfbdcf-at-[206.69.208.22]}
} 8, 11 -- Date: Tue, 22 Sep 2009 08:26:39 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: renaudgeological-at-execulink.com (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Objective lens oil
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
8, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Sep 23 06:37:46 2009
8, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154])
8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8NBbjEm024090
8, 32 -- for {microscopy-at-microscopy.com} ; Wed, 23 Sep 2009 06:37:45 -0500
8, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
8, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n8NBbiOF061080
8, 32 -- for {microscopy-at-microscopy.com} ; Wed, 23 Sep 2009 13:37:44 +0200 (CEST) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr)
8, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3])
8, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
8, 32 -- (No client certificate requested)
8, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 084EA10003F8
8, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Sep 2009 13:22:55 +0200 (CEST)
8, 32 -- Message-ID: {4ABA087E.7060407-at-ipcms.u-strasbg.fr}
8, 32 -- Date: Wed, 23 Sep 2009 13:37:34 +0200
8, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
8, 32 -- User-Agent: Thunderbird 2.0.0.23 (X11/20090817)
8, 32 -- MIME-Version: 1.0
8, 32 -- To: Microscopy-at-microscopy.com
8, 32 -- Subject: Re: [Microscopy] viaWWW: Objective lens oil
8, 32 -- References: {200909221330.n8MDUj2O024138-at-ns.microscopy.com}
8, 32 -- In-Reply-To: {200909221330.n8MDUj2O024138-at-ns.microscopy.com}
8, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 32 -- Content-Transfer-Encoding: 8bit
8, 32 -- X-IPCMS-MailScanner: Found to be clean
8, 32 -- X-IPCMS-MailScanner-SpamScore: s
8, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
8, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.154]); Wed, 23 Sep 2009 13:37:44 +0200 (CEST)
8, 32 -- X-Virus-Scanned: ClamAV 0.94.2/9826/Wed Sep 23 13:06:01 2009 on mr4.u-strasbg.fr
8, 32 -- X-Virus-Status: Clean
8, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST
8, 32 -- autolearn=disabled version=3.2.5
8, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Wed, 23 Sep 2009 10:14:05 -0500
Subject: [Microscopy] SEM protocol for Bees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
anybody out there who can give me some advice how to process bees for
high resolution SEM work?
Normally I would go through 3% and 5% Glutaraldehyde fixation and then
ethanol / water dehydration and critical point drying.
If there is a better protocol I would be very fond of getting it, also
the minimum time of complete insects in each step...
Also: Has anybody tried to image bee wax without using cryo?

Thanks,
Stefan


--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
5, 20 -- From stefan.diller-at-t-online.de Wed Sep 23 10:14:04 2009
5, 20 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8NFE4AY015605
5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 23 Sep 2009 10:14:04 -0500
5, 20 -- Received: from fwd04.aul.t-online.de
5, 20 -- by mailout07.t-online.de with smtp
5, 20 -- id 1MqTXV-0006o4-03; Wed, 23 Sep 2009 17:13:53 +0200
5, 20 -- Received: from [192.168.2.101] (T5n-NoZYZh-rFWmNZA3hWgpCF4RU1aShEEH6tvfW6+agf7XXUFfrNA2z19gWXioQcJ-at-[93.222.103.209]) by fwd04.aul.t-online.de
5, 20 -- with esmtp id 1MqTXM-1L7zfc0; Wed, 23 Sep 2009 17:13:44 +0200
5, 20 -- Message-ID: {4ABA3B27.7060907-at-t-online.de}
5, 20 -- Date: Wed, 23 Sep 2009 17:13:43 +0200
5, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
5, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
5, 20 -- MIME-Version: 1.0
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- Subject: SEM protocol for Bees
5, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
5, 20 -- Content-Transfer-Encoding: 7bit
5, 20 -- X-ID: T5n-NoZYZh-rFWmNZA3hWgpCF4RU1aShEEH6tvfW6+agf7XXUFfrNA2z19gWXioQcJ
5, 20 -- X-TOI-MSGID: e9b9b557-3529-49d1-b8fb-e31056c36098
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 25 Sep 2009 09:48:09 -0500
Subject: [Microscopy] Leica EMPact HPF control screen needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Gjønnes Medal in Electron Crystallography recognizes an
outstanding contribution to the field of electron crystallography. The
Gjønnes Medal is accompanied by a certificate and funding to present
an invited Keynote Lecture at the triennial International Congress of
Crystallography.

This award is managed by the Awards Sub-committee of the Commission on
Electron Crystallography (CEC) of the IUCr, see
http://www.numis.northwestern.edu/IUCR_CEC. This Sub-committee is
responsible for soliciting and evaluating and recommending a nominee
to members of the CEC for final approval, or recommending that no
award be made.

Nomination for the Gjønnes Medal is open to scientists and engineers
in all areas of electron crystallography defined in the broadest
context as the branch of science that uses electron scattering and
imaging to study the structure of matter. Nominees of any nationality
are eligible. It is expected that the nomination will be for a single
person. However, since breakthrough innovations often involve more
than one individual, the nomination can be for two or three people,
maximum. In the case of multiple nominees, it must be shown that each
person contributed roughly equally to the innovation.

The Award will not be bestowed in absentia except in extraordinary
circumstances. Current members of the CEC, members of the IUCr
Executive Committee and previous recipients of the Gjønnes Medal are
not eligible.

The next Gjønnes Medal will be awarded at the Madrid Congress in August 2011.
The nomination packet should be sent to the Chair of the CEC,
Professor L. D. Marks (L-marks-at-northwestern.edu), and should include:
• A letter of not more than 3,000 characters evaluating the nominee's
qualifications in the field of electron crystallography and
identifying the specific work to be recognized. There is no nomination
form, so this letter is considered the nomination "application".
• Curriculum vitae of the nominee.
• A list of the most important publications of the nominee.
• At least two, but no more than four, seconding letters.
The deadline for receipt of nominations is 30 April 2010

--
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60208, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L-marks at northwestern dot edu
Web: www.numis.northwestern.edu
EMM2007 http://ns.crys.ras.ru/EMMM07/
Commission on Electron Diffraction of IUCR
www.numis.northwestern.edu/IUCR_CED


==============================Original Headers==============================
7, 33 -- From marksmsa-at-gmail.com Thu Sep 24 13:35:57 2009
7, 33 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.158])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8OIZuxq015206
7, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Sep 2009 13:35:57 -0500
7, 33 -- Received: by fg-out-1718.google.com with SMTP id e12so661582fga.0
7, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Sep 2009 11:35:56 -0700 (PDT)
7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 33 -- d=gmail.com; s=gamma;
7, 33 -- h=domainkey-signature:mime-version:received:date:message-id:subject
7, 33 -- :from:to:content-type:content-transfer-encoding;
7, 33 -- bh=qfMoR67Oax7T3QyfjIdnmZFQGFZ0PfiQj6Ym4e0BsMQ=;
7, 33 -- b=FVEv+plAmF6EsSu5YE2efDpRi+vxc6eCoGEgOoieUNZZa0x+br2MKPd4z1wA21VSUY
7, 33 -- AK8W+tEH9SBiOZ3hBxjgjYHvxyDuT9wnllzkgB2KSkXv3Qq/D0hWVl0BHT/cuhqKs46/
7, 33 -- OCi7Ft0BbaSLHFhi6TqHzs3RSI8PEllB0pQkU=
7, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 33 -- d=gmail.com; s=gamma;
7, 33 -- h=mime-version:date:message-id:subject:from:to:content-type
7, 33 -- :content-transfer-encoding;
7, 33 -- b=Bz1WMT9U+0Vxn1yw0DKD19Iy4BQdI4j8IrYGibF6iagwf6P//qzls+yMFjrRDLaxX8
7, 33 -- 1KsplCBy+ACPU7XVtYO96T9///sPfMhKp9sLtVpXkUlpxRw0X2oENsuTgfl8IuAzliJP
7, 33 -- 9LsHBtsL6Oh0ZSqHAKObd9GCCjF1RfPf+lp9w=
7, 33 -- MIME-Version: 1.0
7, 33 -- Received: by 10.239.141.142 with SMTP id c14mr359274hba.1.1253817356154; Thu,
7, 33 -- 24 Sep 2009 11:35:56 -0700 (PDT)
7, 33 -- Date: Thu, 24 Sep 2009 13:35:55 -0500
7, 33 -- Message-ID: {e13ba6260909241135v3286560dn443450d3fd5a7e99-at-mail.gmail.com}
7, 33 -- Subject: =?ISO-8859-1?Q?Gj=F8nnes_Medal_in_Electron_Crystallography_=2D_call_fo?=
7, 33 -- =?ISO-8859-1?Q?r_nominations?=
7, 33 -- From: L Marks {marksmsa-at-gmail.com}
7, 33 -- To: microscopy {Microscopy-at-microscopy.com}
7, 33 -- Content-Type: text/plain; charset=windows-1252
7, 33 -- Content-Transfer-Encoding: 8bit
7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8OIZuxq015206
==============================End of - Headers==============================

From aus1010-at-btinternet.com Thu Sep 24 22:34:27 2009
Return-Path: {aus1010-at-btinternet.com}
Received: from web87008.mail.ird.yahoo.com (web87008.mail.ird.yahoo.com [87.248.114.60])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8P3YQG6019129
for {MicroscopyListserverArchive-at-microscopy.com} ; Thu, 24 Sep 2009 22:34:27 -0500
Received: (qmail 52494 invoked by uid 60001); 25 Sep 2009 03:34:26 -0000
DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=btinternet.com; s=s1024; t=1253849666; bh=6NJjFiTRaUbaZLEH2cSFcwIvIWkTJ+5HidX66cFAEOE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=Ao0AkGTlZMGwRqbsERNE1Ew8uiTQe4bUBT2cqO+QIS0Y75M1PDR/s5bs4qswdNmlSv6hn2wqlUcN1+YY8s8O02mnqIB2lPzVKsOuPPremEVNL6h4VK/a6dc+Q/mY2zYrj1znvSc4Ui82aigyJlT4jyeCZb3m78/ZDp3e7T9g6a0=
DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
s=s1024; d=btinternet.com;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type;
b=Fdx11DFc0NQYOklZi/L+65pJwYoIaPZyiAOhf/vLBewifG/yywDcn8NU/JRzMfNtKPDhXAj1/oQaRxSRn9rtXMNK0nU5YpU6BBLMoeRIyIQa/9x+wmoo5HzkF7WCHO7xNh2aXdPfK6z8Gq3r6fWKvwYvdAKRXF7atKdWrtv4+6g=;
Message-ID: {512042.47314.qm-at-web87008.mail.ird.yahoo.com}
X-YMail-OSG: FSlEfwYVM1kNSiGh2za7YxdYBx29d9BqS29y.IYxxuyOfVvxjlFXzPN6KMI5NckHlPqaw_yDoHYEsREu.DBSESIfhgVCqcSsJVJm7DDjfEWkkNwBDFaX8k22v.yqGR_nAQ9pS.D2GHknnGnfjgkrIwqnAypE0nFIYkorInVqe8e5j7h2eGE.3uzm.u4cEo8l_BdksR1KrUL3x1KQPzugVgk2edQi1WUAn2ahA2UUEz6LxSdvyt7Dl14GWDxqAcoDZ7NkBq.MY10heQ7Hg2v8mrLiHwSpYhstF86_xg7IK1d4C5GUpmgsDlh0ZcxrsI.0xF4_DYoANgx4REFKBPuD2UeSB7UaZ4rHzAnAFHN0REbDFgVoMC2.Xab0vgMwUnDO9LGAM5rz9Go_ASmFfTrWK4e2b.OeuBHufx66S8jp9l7.LPLDtRMAMTrZG1rYZ5vJRvn6aTN1_mgU8vZuYsvYHLjdNuhabsJjH2KURtlXo_EFZFKla.2T20RcufAWvV92_yk9W0Zd13_abpWzX9wVinGigaKnIpbHve7Tp3fdRhOZ0LyZIdd_qxOIxivCN0WJL3AkSRR1d.udOmH_hqzyh_Q32HWf
Received: from [62.56.140.209] by web87008.mail.ird.yahoo.com via HTTP; Fri, 25 Sep 2009 03:34:26 GMT
X-Mailer: YahooMailClassic/7.0.14 YahooMailWebService/0.7.347.3

Dear Collective,

We have a Leica EMPact 1 (early model) high-pressure freezer with an inoperative touch control screen that cannot be repaired. We are trying to locate a used replacement for this screen, which is currently the only way we can afford to bring it back online. Sooooo, if anyone out there might have a mothballed EMPact 1 with a functioning screen, we would be extremely happy to open a line of communications with you concerning purchase of certain scarce parts.

We remain,

Eternally hopeful,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



==============================Original Headers==============================
7, 30 -- From TindallR-at-missouri.edu Fri Sep 25 09:48:08 2009
7, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8PEm8ZV018268
7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 09:48:08 -0500
7, 30 -- X-IronPort-Anti-Spam-Filtered: true
7, 30 -- X-IronPort-Anti-Spam-Result: ApoEAJF0vErRauUo/2dsb2JhbADHVwEJhn2ISoJKgVQF
7, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
7, 30 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 25 Sep 2009 09:48:02 -0500
7, 30 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 30 -- Fri, 25 Sep 2009 09:47:59 -0500
7, 30 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
7, 30 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 25 Sep 2009
7, 30 -- 09:47:56 -0500
7, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
7, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
7, 30 -- Date: Fri, 25 Sep 2009 09:47:55 -0500
7, 30 -- Subject: Leica EMPact HPF control screen needed
7, 30 -- Thread-Topic: Leica EMPact HPF control screen needed
7, 30 -- Thread-Index: Aco97yrSnNtjJjyeRdKqeHdDTbTlqw==
7, 30 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129877C912-at-UM-EMAIL06.um.umsystem.edu}
7, 30 -- Accept-Language: en-US
7, 30 -- Content-Language: en-US
7, 30 -- X-MS-Has-Attach:
7, 30 -- X-MS-TNEF-Correlator:
7, 30 -- acceptlanguage: en-US
7, 30 -- Content-Type: text/plain; charset="us-ascii"
7, 30 -- MIME-Version: 1.0
7, 30 -- X-OriginalArrivalTime: 25 Sep 2009 14:47:59.0238 (UTC) FILETIME=[2CD90A60:01CA3DEF]
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8PEm8ZV018268
==============================End of - Headers==============================




From: doc.vrdoljak-at-gmail.com
Date: Fri, 25 Sep 2009 13:03:07 -0500
Subject: [Microscopy] cryo-sem question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Anyone use a Gatan Altos to freeze and image wet/oily samples for
cryo-sem? I'm having trouble finding the right holder for my samples
which have the consistency of thin oil. The sample tends to roll off
hats, rivets, and not give me a good drop of material for freezing.

==============================Original Headers==============================
1, 29 -- From doc.vrdoljak-at-gmail.com Fri Sep 25 13:03:07 2009
1, 29 -- Received: from mail-iw0-f179.google.com (mail-iw0-f179.google.com [209.85.223.179])
1, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8PI375x009323
1, 29 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 13:03:07 -0500
1, 29 -- Received: by iwn9 with SMTP id 9so1663727iwn.10
1, 29 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 11:03:07 -0700 (PDT)
1, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 29 -- d=gmail.com; s=gamma;
1, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
1, 29 -- :from:to:content-type;
1, 29 -- bh=RfNBdIUP4iYanYJYRqZmtIMfv1t0L098yCOa4gnW2oc=;
1, 29 -- b=JzRxvdvnNu+74l5SEBUm3eW/kH8DZ8/bamP/hxydHdTM733N8gnmM26qzQt5YGutRa
1, 29 -- x/stA7ofZFogUM3sRcYeG6CvyafqUdLPd3z1pMuV4NWV4SESbNf2coR8kwbY4RhWRHJ/
1, 29 -- jlCKfpHlsDzX8uPYl1Q/LfAI3gPIhttnMYZAw=
1, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
1, 29 -- d=gmail.com; s=gamma;
1, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
1, 29 -- b=RUkkwe2jYU/dIfT7axf48ZlhpbeGMCMWCiSsezb5V8PA/Mbvln/GPEffZNP2J3tEGb
1, 29 -- CHGjYJiorrLjoiZmjZQkGNmoJ10kw1+W7yNfER6UVifSGseE/bBGH4g73Z16WCJ2GEAy
1, 29 -- DMFJSJyUzYkAbG7XKT7K+se3PCI3Q2vkuYdWI=
1, 29 -- MIME-Version: 1.0
1, 29 -- Received: by 10.231.22.38 with SMTP id l38mr773681ibb.34.1253901786947; Fri,
1, 29 -- 25 Sep 2009 11:03:06 -0700 (PDT)
1, 29 -- Date: Fri, 25 Sep 2009 11:03:06 -0700
1, 29 -- Message-ID: {fb272960909251103g7d9b9accu783d7f497b910c0d-at-mail.gmail.com}
1, 29 -- Subject: cryo-sem question
1, 29 -- From: Gordon Vrdoljak {doc.vrdoljak-at-gmail.com}
1, 29 -- To: microscopy-at-microscopy.com
1, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: margaret.dienelt-at-ars.usda.gov
Date: Fri, 25 Sep 2009 15:24:31 -0500
Subject: [Microscopy] viaWWW: LR White problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Try using a thin walled drinking straw mounted within a cryo holder and
syringe the fluid into it? I have used similar technique for other
difficult cryo specimens.

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {doc.vrdoljak-at-gmail.com}
To: {protrain-at-emcourses.com}
Sent: Friday, September 25, 2009 7:04 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both margaret.dienelt-at-ars.usda.gov as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: margaret.dienelt-at-ars.usda.gov
Name: Margaret Dienelt

Organization: National Arboretum, USDA

Title-Subject: [Filtered] LR White problems

Question: Hello Everyone,

I've recently returned to immunolabeling after a 10 year break and
have run into a problem with LR White that I don't remember. I'm
embedding virus-infected tobacco leaves but I suddenly can't see the
tissue in the block to section it. Since samples aren't treated with
osmium, it was never easy, but I don't recall it being the hit or
miss matter it is now.

When I finally do get sections, the cell walls appear to be bleached
- they're a clear white, much lighter than the cell contents and even
lighter than the surrounding plastic. This is with uranyl acetate
and lead citrate staining that in the past gave me very lightly
stained cell walls.

To make matters worse, the quality of preservation is totally
unacceptable and labeling tests have been uniformly unsuccessful,
even positive controls.

My fixation/embedding protocol is one I used many times in the past
without problem. I fix in 4% formaldehyde and 0.3% glutaraldehyde
in phosphate buffer, and dehydrate samples in a graded series of
alcohol.. I've tested both the formaldehyde and glutaraldehyde in
conventional osmium treated LX112 embeddings and preservation is okay
so it seems to be the LR White.

I'm wondering: has anyone else had this problem, and if so, what did
you do about it? I checked the date on the LR White and it's still
fresh.

Another question: has anyone had success immunolabeling tissue fixed
in osmium and/or embedded in LX112?

I really appreciate any advice you can give me.

Thanks!


Margaret

Margaret Dienelt
Plant Pathologist
Electron Microscopy
FNPRU, National Arboretum, ARS, USDA

Bldg 010A Rm 230 BARC-W
10300 Baltimore Avenue
Beltsville, MD 20705

301-504-6097 (phone)
301-504-5096 (fax)



Login Host: 199.133.19.246
---------------------------------------------------------------------------

==============================Original Headers==============================
21, 11 -- From zaluzec-at-microscopy.com Fri Sep 25 15:24:31 2009
21, 11 -- Received: from [58.98.37.126] ([130.202.238.72])
21, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8PKORuL012452
21, 11 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 15:24:29 -0500
21, 11 -- Mime-Version: 1.0
21, 11 -- Message-Id: {p0624080bc6e2d76a686c-at-[58.98.37.126]}
21, 11 -- Date: Sat, 26 Sep 2009 05:24:27 +0900
21, 11 -- To: microscopy-at-microscopy.com
21, 11 -- From: margaret.dienelt-at-ars.usda.gov (by way of MicroscopyListserver)
21, 11 -- Subject: viaWWW: LR White problems
21, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Fri, 25 Sep 2009 16:09:31 -0500
Subject: [Microscopy] Re: viaWWW: LR White problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Margaret,
This won't help your preservation problems but as far as
visibility goes, I have found in my work with methacrylate resins
that adding a microL or two of ethanolic fast green at the 100%
ethanol stage stains plant material well and does not interfere with
immunowork. I think I make a 1 or 2 % fast green solution.
Good luck with the problem.

Tobias
}
} Email: margaret.dienelt-at-ars.usda.gov
} Name: Margaret Dienelt
}
} Organization: National Arboretum, USDA
}
} Title-Subject: [Filtered] LR White problems
}
} Question: Hello Everyone,
}
} I've recently returned to immunolabeling after a 10 year break and
} have run into a problem with LR White that I don't remember. I'm
} embedding virus-infected tobacco leaves but I suddenly can't see the
} tissue in the block to section it. Since samples aren't treated with
} osmium, it was never easy, but I don't recall it being the hit or
} miss matter it is now.
}
} When I finally do get sections, the cell walls appear to be bleached
} - they're a clear white, much lighter than the cell contents and even
} lighter than the surrounding plastic. This is with uranyl acetate
} and lead citrate staining that in the past gave me very lightly
} stained cell walls.
}
} To make matters worse, the quality of preservation is totally
} unacceptable and labeling tests have been uniformly unsuccessful,
} even positive controls.
}
} My fixation/embedding protocol is one I used many times in the past
} without problem. I fix in 4% formaldehyde and 0.3% glutaraldehyde
} in phosphate buffer, and dehydrate samples in a graded series of
} alcohol.. I've tested both the formaldehyde and glutaraldehyde in
} conventional osmium treated LX112 embeddings and preservation is okay
} so it seems to be the LR White.
}
} I'm wondering: has anyone else had this problem, and if so, what did
} you do about it? I checked the date on the LR White and it's still
} fresh.
}
} Another question: has anyone had success immunolabeling tissue fixed
} in osmium and/or embedded in LX112?
}
} I really appreciate any advice you can give me.
}
} Thanks!
}
}
} Margaret
}
} Margaret Dienelt
} Plant Pathologist
} Electron Microscopy
} FNPRU, National Arboretum, ARS, USDA
}
} Bldg 010A Rm 230 BARC-W
} 10300 Baltimore Avenue
} Beltsville, MD 20705
}
} 301-504-6097 (phone)
} 301-504-5096 (fax)
}
}
}
} Login Host: 199.133.19.246
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 21, 11 -- From zaluzec-at-microscopy.com Fri Sep 25 15:24:31 2009
} 21, 11 -- Received: from [58.98.37.126] ([130.202.238.72])
} 21, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id n8PKORuL012452
} 21, 11 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009
} 15:24:29 -0500
} 21, 11 -- Mime-Version: 1.0
} 21, 11 -- Message-Id: {p0624080bc6e2d76a686c-at-[58.98.37.126]}
} 21, 11 -- Date: Sat, 26 Sep 2009 05:24:27 +0900
} 21, 11 -- To: microscopy-at-microscopy.com
} 21, 11 -- From: margaret.dienelt-at-ars.usda.gov (by way of MicroscopyListserver)
} 21, 11 -- Subject: viaWWW: LR White problems
} 21, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
4, 21 -- From baskin-at-bio.umass.edu Fri Sep 25 16:09:31 2009
4, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8PL9VJJ028732
4, 21 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 16:09:31 -0500
4, 21 -- Received: from [172.30.52.170] (eutopia.bio.umass.edu [128.119.55.30])
4, 21 -- (authenticated bits=0)
4, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n8PL9Poi020955
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
4, 21 -- Fri, 25 Sep 2009 17:09:26 -0400 (EDT)
4, 21 -- Mime-Version: 1.0
4, 21 -- Message-Id: {p06240520c6e2e160926a-at-[172.30.52.170]}
4, 21 -- In-Reply-To: {200909252025.n8PKP1qa013241-at-ns.microscopy.com}
4, 21 -- References: {200909252025.n8PKP1qa013241-at-ns.microscopy.com}
4, 21 -- Date: Fri, 25 Sep 2009 17:09:24 -0400
4, 21 -- To: margaret.dienelt-at-ars.usda.gov
4, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 21 -- Subject: Re: [Microscopy] viaWWW: LR White problems
4, 21 -- Cc: microscopy-at-microscopy.com
4, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Fri, 25 Sep 2009 17:09:26 -0400 (EDT)
4, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 25 Sep 2009 16:33:01 -0500
Subject: [Microscopy] Re: viaWWW: LR White problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Margaret,

Did you use an accelerator with LR White? The accelerator is benzoyl
peroxide and that could explain a lot of your symptoms.

If no accelerator was used, then we have to consider other
possibilities: inadequate fixation by the aldehydes, extraction by
various organic solvents used for dehydration, length of time in
various liquids (alcohols, resins, etc.).

John Bozzola

On Fri, Sep 25, 2009 at 3:25 PM, {margaret.dienelt-at-ars.usda.gov} wrote:
}
}
} Title-Subject: [Filtered] LR White problems
}

} When I finally do get sections, the cell walls appear to be bleached
} - they're a clear white, much lighter than the cell contents and even
} lighter than the surrounding plastic.  This is with uranyl acetate
} and lead citrate staining that in the past gave me very lightly
} stained cell walls.
}
} To make matters worse, the quality of preservation is totally
} unacceptable and labeling tests have been uniformly unsuccessful,
} even positive controls.

--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901


==============================Original Headers==============================
8, 19 -- From bozzola-at-siu.edu Fri Sep 25 16:33:01 2009
8, 19 -- Received: from mail-px0-f187.google.com (mail-px0-f187.google.com [209.85.216.187])
8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8PLX18R011141
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 16:33:01 -0500
8, 19 -- Received: by pxi17 with SMTP id 17so4119997pxi.21
8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Sep 2009 14:33:00 -0700 (PDT)
8, 19 -- MIME-Version: 1.0
8, 19 -- Received: by 10.115.117.39 with SMTP id u39mr551036wam.116.1253914380826; Fri,
8, 19 -- 25 Sep 2009 14:33:00 -0700 (PDT)
8, 19 -- In-Reply-To: {200909252025.n8PKPGPM013766-at-ns.microscopy.com}
8, 19 -- References: {200909252025.n8PKPGPM013766-at-ns.microscopy.com}
8, 19 -- Date: Fri, 25 Sep 2009 16:33:00 -0500
8, 19 -- Message-ID: {ebc2299e0909251433u340df791k2ca87d62807ade12-at-mail.gmail.com}
8, 19 -- Subject: Re: [Microscopy] viaWWW: LR White problems
8, 19 -- From: John Bozzola {bozzola-at-siu.edu}
8, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
8, 19 -- Content-Type: text/plain; charset=UTF-8
8, 19 -- Content-Transfer-Encoding: 8bit
8, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8PLX18R011141
==============================End of - Headers==============================




From: joexray-at-cinci.rr.com
Date: Sat, 26 Sep 2009 19:57:32 -0500
Subject: [Microscopy] JoeXray LLC is moving to new location

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Please note that we will be moving our office October 1st 2009. Our telephone number will change to a new number, in the interim please call my cell number of 936 554-2628 and we will also start using the following new e-mail address:

joe.ullmer-at-joexray.net

Our physical address will be:

JoeXray LLC
4066 Ranch Drive
Beavercreek, OH 45432

Our update will be sent with new numbers as soon as they are established, if necessary you may check our website for further updates.

Thank you,

Joe Ullmer

JoeXray LLC
4066 Ranch Drive
Beavercreek, OH 45432
OFFICE / FAX: 937 550-9224-Until Sept 30
Cell: 937 554-2628
joe.ullmer-at-joexray.net
www.joexray.net

==============================Original Headers==============================
9, 19 -- From joexray-at-cinci.rr.com Sat Sep 26 19:57:31 2009
9, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8R0vVKw017467
9, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Sep 2009 19:57:31 -0500
9, 19 -- Received: from hrndva-web22-z01 ([10.128.132.113])
9, 19 -- by hrndva-smta01.mail.rr.com with ESMTP
9, 19 -- id {20090927005731292.DZBU8074-at-hrndva-smta01.mail.rr.com}
9, 19 -- for {Microscopy-at-microscopy.com} ; Sun, 27 Sep 2009 00:57:31 +0000
9, 19 -- Message-ID: {20090927005731.1IMDH.636521.root-at-hrndva-web22-z01}
9, 19 -- Date: Sat, 26 Sep 2009 20:57:31 -0400
9, 19 -- From: {joexray-at-cinci.rr.com}
9, 19 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
9, 19 -- Subject: JoeXray LLC is moving to new location
9, 19 -- MIME-Version: 1.0
9, 19 -- Content-Type: text/plain; charset=utf-8
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- X-Priority: 3 (Normal)
9, 19 -- Sensitivity: Normal
9, 19 -- X-Originating-IP:
==============================End of - Headers==============================




From: pveril-at-apae.uth.gr
Date: Mon, 28 Sep 2009 09:03:58 -0500
Subject: [Microscopy] viaWWW: Put speciments on to a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pveril-at-apae.uth.gr as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pveril-at-apae.uth.gr
Name: Panos Verillis

Organization: University Thessaly

Title-Subject: [Filtered] Put speciments on to a grid

Question: Hi all. I work with TEM for biological specimens. I used to
have grids coated with carbon. So i had only to touch the specimens
with the carbon side in order to grub them from the ultramicrotome' s
knife. Now i have grids with out carbon film. Witch is the best way
to grub my specimens from the knife. I tried but to put the grid
under the specimens but most of them were destroyed.

Login Host: 83.212.32.220
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Sep 28 09:03:57 2009
6, 11 -- Received: from [172.22.2.6] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8SE3sS6014396
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 09:03:55 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c6e672ae0c7f-at-[172.22.2.6]}
6, 11 -- Date: Mon, 28 Sep 2009 23:03:51 +0900
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: pveril-at-apae.uth.gr (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Put speciments on to a grid
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 28 Sep 2009 09:44:29 -0500
Subject: [Microscopy] Re: viaWWW: Put speciments on to a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
When I use to cut TEM thin sections of polymer I liked EMS's perfect loop.
Try googling: perfect loop TEM.


By the way, I don't work for google or EMS.............
Stay safe.....Frank




pveril-at-apae.uth.g
r
To
09/28/2009 10:14 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] viaWWW: Put speciments
pveril-at-apae.uth.g on to a grid
r












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both pveril-at-apae.uth.gr as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pveril-at-apae.uth.gr
Name: Panos Verillis

Organization: University Thessaly

Title-Subject: [Filtered] Put speciments on to a grid

Question: Hi all. I work with TEM for biological specimens. I used to
have grids coated with carbon. So i had only to touch the specimens
with the carbon side in order to grub them from the ultramicrotome' s
knife. Now i have grids with out carbon film. Witch is the best way
to grub my specimens from the knife. I tried but to put the grid
under the specimens but most of them were destroyed.

Login Host: 83.212.32.220
---------------------------------------------------------------------------

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Sep 28 09:03:57 2009
6, 11 -- Received: from [172.22.2.6] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n8SE3sS6014396
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2009
09:03:55 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c6e672ae0c7f-at-[172.22.2.6]}
6, 11 -- Date: Mon, 28 Sep 2009 23:03:51 +0900
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: pveril-at-apae.uth.gr (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Put speciments on to a grid
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
25, 22 -- From frank_karl-at-lincolnelectric.com Mon Sep 28 09:44:29 2009
25, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
25, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8SEiTmh030699
25, 22 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 09:44:29 -0500
25, 22 -- In-Reply-To: {200909281414.n8SEEFqs026758-at-ns.microscopy.com}
25, 22 -- Subject: Re: [Microscopy] viaWWW: Put speciments on to a grid
25, 22 -- To: pveril-at-apae.uth.gr, Microscopy-at-microscopy.com
25, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
25, 22 -- Message-ID: {OF2BE06FFF.BE953862-ON8525763F.0050C88F-8525763F.0050F271-at-lincolnelectric.com}
25, 22 -- Date: Mon, 28 Sep 2009 10:44:44 -0400
25, 22 -- From: Frank_Karl-at-lincolnelectric.com
25, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 09/28/2009 10:44:16 AM,
25, 22 -- CD-MIME complete at 09/28/2009 10:44:16 AM,
25, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 09/28/2009 10:44:16 AM,
25, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 09/28/2009 10:44:16 AM,
25, 22 -- Serialize complete at 09/28/2009 10:44:16 AM
25, 22 -- MIME-Version: 1.0
25, 22 -- Content-Type: text/plain;
25, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Mon, 28 Sep 2009 11:47:37 -0500
Subject: [Microscopy] Re: viaWWW: Put speciments on to a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I used to touch down from the top as you did with plain Cu grids--
worked beautifully.
Roseann






On Sep 28, 2009, at 7:15 AM, pveril-at-apae.uth.gr wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both pveril-at-apae.uth.gr as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: pveril-at-apae.uth.gr
} Name: Panos Verillis
}
} Organization: University Thessaly
}
} Title-Subject: [Filtered] Put speciments on to a grid
}
} Question: Hi all. I work with TEM for biological specimens. I used to
} have grids coated with carbon. So i had only to touch the specimens
} with the carbon side in order to grub them from the ultramicrotome' s
} knife. Now i have grids with out carbon film. Witch is the best way
} to grub my specimens from the knife. I tried but to put the grid
} under the specimens but most of them were destroyed.
}


==============================Original Headers==============================
9, 23 -- From RCsencsits-at-lbl.gov Mon Sep 28 11:47:37 2009
9, 23 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8SGlZHa017554
9, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 11:47:36 -0500
9, 23 -- X-Ironport-SBRS: None
9, 23 -- X-IronPort-Anti-Spam-Filtered: true
9, 23 -- X-IronPort-Anti-Spam-Result: AjYHAGOFwEqD8yD6/2dsb2JhbACCE9UchB4F
9, 23 -- X-IronPort-AV: E=Sophos;i="4.44,467,1249282800";
9, 23 -- d="scan'208";a="102968057"
9, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov ([131.243.32.250])
9, 23 -- by ironport2.lbl.gov with ESMTP/TLS/AES128-SHA; 28 Sep 2009 09:47:17 -0700
9, 23 -- Cc: Microscopy-at-microscopy.com
9, 23 -- Message-Id: {A7937C4D-98EF-4166-A0FA-75D896288933-at-lbl.gov}
9, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov}
9, 23 -- To: pveril-at-apae.uth.gr
9, 23 -- In-Reply-To: {200909281415.n8SEFSZd028781-at-ns.microscopy.com}
9, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 23 -- Content-Transfer-Encoding: 7bit
9, 23 -- Mime-Version: 1.0 (Apple Message framework v936)
9, 23 -- Subject: Re: [Microscopy] viaWWW: Put speciments on to a grid
9, 23 -- Date: Mon, 28 Sep 2009 09:47:15 -0700
9, 23 -- References: {200909281415.n8SEFSZd028781-at-ns.microscopy.com}
9, 23 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Mon, 28 Sep 2009 13:03:30 -0500
Subject: [Microscopy] Finest Grained Sputtered Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I would like to get some suggestions for choosing a target for the finest
grained coating that can be sputtered using a Denton Desk II sputter coater.
Thanks.

John Mardinly
Western Digital



==============================Original Headers==============================
6, 25 -- From John.Mardinly-at-wdc.com Mon Sep 28 13:03:30 2009
6, 25 -- Received: from wdscexfe01.sc.wdc.com (wdscexfe01.sc.wdc.com [129.253.170.53])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8SI3UK8004423
6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 13:03:30 -0500
6, 25 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Mon, 28 Sep 2009 11:02:32 -0700
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="us-ascii"
6, 25 -- Subject: Finest Grained Sputtered Film
6, 25 -- Date: Mon, 28 Sep 2009 11:02:31 -0700
6, 25 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E11815-at-wdksjexbe01.msj.wdc.com}
6, 25 -- In-Reply-To: {34061960C62E274C8AF1DCAA6565555805E11814-at-wdksjexbe01.msj.wdc.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: Finest Grained Sputtered Film
6, 25 -- Thread-Index: AcpAZZvU30NXgDGOTTiytNWWSmufuAAABOqQ
6, 25 -- References: {34061960C62E274C8AF1DCAA6565555805E11814-at-wdksjexbe01.msj.wdc.com}
6, 25 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 28 Sep 2009 18:02:32.0506 (UTC) FILETIME=[D9E5A1A0:01CA4065]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8SI3UK8004423
==============================End of - Headers==============================




From: jerzy.gazda-at-ceriumlabs.com
Date: Mon, 28 Sep 2009 13:18:14 -0500
Subject: [Microscopy] Finest Grained Sputtered Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,
We use Baltec coaters that might have different sputtering currents, but
at low currents, Cr gives the best coverage and smallest grain. The
disadvantages are: quick oxidation ( { 2 weeks usefulness of specimens)
and with some soft polymeric materials (resist) we can get implantation
of Cr under the surface and distortions.

Ir was also reported to work better that Au/Pt/Pd ...

Hope this helps,

Jerzy
www.ceriumlabs.com

-----Original Message-----
X-from: John.Mardinly-at-wdc.com [mailto:John.Mardinly-at-wdc.com]
Sent: Monday, September 28, 2009 1:12 PM
To: Gazda, Jerzy



I would like to get some suggestions for choosing a target for the
finest
grained coating that can be sputtered using a Denton Desk II sputter
coater.
Thanks.

John Mardinly
Western Digital



==============================Original
Headers==============================
6, 25 -- From John.Mardinly-at-wdc.com Mon Sep 28 13:03:30 2009
6, 25 -- Received: from wdscexfe01.sc.wdc.com (wdscexfe01.sc.wdc.com
[129.253.170.53])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n8SI3UK8004423
6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2009
13:03:30 -0500
6, 25 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by
wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Mon, 28 Sep 2009 11:02:32 -0700
6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
6, 25 -- Content-class: urn:content-classes:message
6, 25 -- MIME-Version: 1.0
6, 25 -- Content-Type: text/plain;
6, 25 -- charset="us-ascii"
6, 25 -- Subject: Finest Grained Sputtered Film
6, 25 -- Date: Mon, 28 Sep 2009 11:02:31 -0700
6, 25 -- Message-ID:
{34061960C62E274C8AF1DCAA6565555805E11815-at-wdksjexbe01.msj.wdc.com}
6, 25 -- In-Reply-To:
{34061960C62E274C8AF1DCAA6565555805E11814-at-wdksjexbe01.msj.wdc.com}
6, 25 -- X-MS-Has-Attach:
6, 25 -- X-MS-TNEF-Correlator:
6, 25 -- Thread-Topic: Finest Grained Sputtered Film
6, 25 -- Thread-Index: AcpAZZvU30NXgDGOTTiytNWWSmufuAAABOqQ
6, 25 -- References:
{34061960C62E274C8AF1DCAA6565555805E11814-at-wdksjexbe01.msj.wdc.com}
6, 25 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
6, 25 -- To: {Microscopy-at-microscopy.com}
6, 25 -- X-OriginalArrivalTime: 28 Sep 2009 18:02:32.0506 (UTC)
FILETIME=[D9E5A1A0:01CA4065]
6, 25 -- Content-Transfer-Encoding: 8bit
6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n8SI3UK8004423
==============================End of -
Headers==============================


==============================Original Headers==============================
16, 29 -- From Jerzy.Gazda-at-spansion.com Mon Sep 28 13:18:14 2009
16, 29 -- Received: from usausmgw01.spansion.com (usausmgw01.spansion.com [12.110.209.161])
16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8SIIDPF022370
16, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 13:18:13 -0500
16, 29 -- X-IronPort-AV: E=McAfee;i="5300,2777,5755"; a="6486745"
16, 29 -- Received: from usausexbh1.spansion.com ([10.248.26.58])
16, 29 -- by usausmgw01.spansion.com with ESMTP; 28 Sep 2009 11:18:13 -0700
16, 29 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959);
16, 29 -- Mon, 28 Sep 2009 13:18:13 -0500
16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 29 -- Content-class: urn:content-classes:message
16, 29 -- MIME-Version: 1.0
16, 29 -- Content-Type: text/plain;
16, 29 -- charset="us-ascii"
16, 29 -- Subject: RE: [Microscopy] Finest Grained Sputtered Film
16, 29 -- Date: Mon, 28 Sep 2009 13:18:12 -0500
16, 29 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158FCA932-at-USAUSEXMBPF1.spansion.com}
16, 29 -- In-Reply-To: {200909281812.n8SICQNE020634-at-ns.microscopy.com}
16, 29 -- X-MS-Has-Attach:
16, 29 -- X-MS-TNEF-Correlator:
16, 29 -- Thread-Topic: [Microscopy] Finest Grained Sputtered Film
16, 29 -- Thread-Index: AcpAZz3VIk9vyFo/Rk6csMSgEifwMgAAE+tQ
16, 29 -- References: {200909281812.n8SICQNE020634-at-ns.microscopy.com}
16, 29 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com}
16, 29 -- To: {John.Mardinly-at-wdc.com}
16, 29 -- Cc: {Microscopy-at-microscopy.com}
16, 29 -- X-OriginalArrivalTime: 28 Sep 2009 18:18:13.0361 (UTC) FILETIME=[0AB0B610:01CA4068]
16, 29 -- Content-Transfer-Encoding: 8bit
16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8SIIDPF022370
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 28 Sep 2009 15:15:36 -0500
Subject: [Microscopy] Re: Finest Grained Sputtered Film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use Pd and Ir (obviously not at the same time) in Denton Desk IV TSC.
The Desk II does not have a turbo (MDP) so you are probably limited
to 30mT. In this case, I would probably opt for Ir if its EDS
signature does not impede your specimen's signatures. With turbo,
I coat at 15mT and cannot see the coating at 600KX. Settings
are 60% power, 120 seconds, 20% tilt/rotate, 15mT.

No way to tell if this is the "finest" grain film. EBSD
on Pd film on field oxide on Si wafer does not work. I
figure that this is because the film is less than 50nm and the
beam just pushes through the film. EDS confirms huge Si peak
with very low Ir or Pd peak(s).

gary g.


At 11:05 AM 9/28/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Mon Sep 28 15:15:36 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8SKFZL6020377
9, 20 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2009 15:15:35 -0500
9, 20 -- Message-Id: {200909282015.n8SKFZL6020377-at-ns.microscopy.com}
9, 20 -- Received: (qmail 25782 invoked from network); 28 Sep 2009 12:58:32 -0700
9, 20 -- Received: by simscan 1.1.0 ppid: 25779, pid: 25780, t: 0.1047s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 28 Sep 2009 12:58:32 -0700
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Mon, 28 Sep 2009 13:15:36 -0700
9, 20 -- To: John.Mardinly-at-wdc.com
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] Finest Grained Sputtered Film
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200909281805.n8SI5L3L007175-at-ns.microscopy.com}
9, 20 -- References: {200909281805.n8SI5L3L007175-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: marie.cantino-at-uconn.edu
Date: Tue, 29 Sep 2009 16:37:58 -0500
Subject: [Microscopy] Dark backgrounds in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking at insects mounted on wires in the SEM. Is there an easy
way to reduce secondary electron emission from the stub below so that
the background appears dark behind the insects? My wires are glued to
an aluminum stub with silver paint.

Marie

Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
4, 16 -- From marie.cantino-at-uconn.edu Tue Sep 29 16:37:58 2009
4, 16 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8TLbwPK031765
4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 16:37:58 -0500
4, 16 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9])
4, 16 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n8TLbvGA003669
4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 17:37:57 -0400
4, 16 -- Message-Id: {F764F989-B7AB-4442-82EF-E43A9A9626B5-at-uconn.edu}
4, 16 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
4, 16 -- To: microscopy-at-microscopy.com
4, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 16 -- Content-Transfer-Encoding: 7bit
4, 16 -- Mime-Version: 1.0 (Apple Message framework v936)
4, 16 -- Subject: Dark backgrounds in SEM
4, 16 -- Date: Tue, 29 Sep 2009 17:37:57 -0400
4, 16 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 29 Sep 2009 16:48:31 -0500
Subject: [Microscopy] Re: Dark backgrounds in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It sounds like the stub is mostly bare Al.
You would need low Z covering material to
reduce SEs. Try putting down some colloidal
Carbon paint. It will likely meniscus over to
the bug wires. But since these are not the
legs or feet, it should not matter.

gary g.


At 02:40 PM 9/29/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 20 -- From gary-at-gaugler.com Tue Sep 29 16:48:31 2009
8, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8TLmU6L008698
8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 16:48:31 -0500
8, 20 -- Message-Id: {200909292148.n8TLmU6L008698-at-ns.microscopy.com}
8, 20 -- Received: (qmail 18900 invoked from network); 29 Sep 2009 15:20:52 -0700
8, 20 -- Received: by simscan 1.1.0 ppid: 18894, pid: 18898, t: 0.0900s
8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
8, 20 -- by smtp1 with SMTP; 29 Sep 2009 15:20:52 -0700
8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
8, 20 -- Date: Tue, 29 Sep 2009 14:48:31 -0700
8, 20 -- To: marie.cantino-at-uconn.edu
8, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
8, 20 -- Subject: Re: [Microscopy] Dark backgrounds in SEM
8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
8, 20 -- In-Reply-To: {200909292140.n8TLeiPA001672-at-ns.microscopy.com}
8, 20 -- References: {200909292140.n8TLeiPA001672-at-ns.microscopy.com}
8, 20 -- Mime-Version: 1.0
8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Tue, 29 Sep 2009 16:54:56 -0500
Subject: [Microscopy] Re: Dark backgrounds in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marie,

We like totally black backgrounds, whenever possible. To achieve this
you need a very flat substratum composed of a low atomic weight. Even
polished aluminum stubs are not totally flat (you will see scratches)
and Al still gives enough signal to make the background lighter than
wanted.

We have good luck mounting specimens on glass coverglasses or pieces
of microscope slide. Even when specimens are coated with Pd/Au, the
background is quite dark, often black.

If you cannot do this with the present specimens, then follow Gary G's
advice and put Carbon paint behind your specimens. This will also give
a dark background.

John Bozzola

On Tue, Sep 29, 2009 at 4:39 PM, {marie.cantino-at-uconn.edu} wrote:
}
}
} I am looking at insects mounted on wires in the SEM.  Is there an easy
} way to reduce secondary electron emission from the stub below so that
} the background appears dark behind the insects?  My wires are glued to
} an aluminum stub with silver paint.


--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901


==============================Original Headers==============================
9, 19 -- From bozzola-at-siu.edu Tue Sep 29 16:54:56 2009
9, 19 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8TLstbt022700
9, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 16:54:55 -0500
9, 19 -- Received: by pzk37 with SMTP id 37so3656102pzk.10
9, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 14:54:55 -0700 (PDT)
9, 19 -- MIME-Version: 1.0
9, 19 -- Received: by 10.115.116.5 with SMTP id t5mr9011754wam.185.1254261295126; Tue,
9, 19 -- 29 Sep 2009 14:54:55 -0700 (PDT)
9, 19 -- In-Reply-To: {200909292139.n8TLdMXm000579-at-ns.microscopy.com}
9, 19 -- References: {200909292139.n8TLdMXm000579-at-ns.microscopy.com}
9, 19 -- Date: Tue, 29 Sep 2009 16:54:55 -0500
9, 19 -- Message-ID: {ebc2299e0909291454j452770e9wa8f3d9ceb2e16e7f-at-mail.gmail.com}
9, 19 -- Subject: Re: [Microscopy] Dark backgrounds in SEM
9, 19 -- From: John Bozzola {bozzola-at-siu.edu}
9, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
9, 19 -- Content-Type: text/plain; charset=UTF-8
9, 19 -- Content-Transfer-Encoding: 8bit
9, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8TLstbt022700
==============================End of - Headers==============================




From: rstallcup-at-zyvex.com
Date: Tue, 29 Sep 2009 17:35:45 -0500
Subject: [Microscopy] Dark backgrounds in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marie,

Drill a hole about 1mm to 2mm in diameter into a sample stub. Make the
hole about 5 mm deep. This will make a great electron trap, what goes in
does not come out. Suspend your sample, bug, over the hole and you will
see a very nice black background. You might want to try different hole
diameter to hole depth arrangements and you can probably fit several
holes on your sample stub.

Richard

........................................................................
Richard E. Stallcup II, PhD
Applications Manager,
NanoWorks® Tools; Senior Scientist

Zyvex Instruments, LLC
Providing Nanotechnology Solutions – Today®

t: 972.792.1619
c: 972-522-9870
f: 972.235.7882
e: rstallcup-at-zyvex.com
w: www.zyvex.com
........................................................................
Notice of Confidentiality:

The information contained in this transmission
is privileged and confidential and is intended only
for the use of the addressee(s).

This e-mail is sent for business reasons only and
should be considered confidential.
........................................................................




marie.cantino-at-uconn.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I am looking at insects mounted on wires in the SEM. Is there an easy
} way to reduce secondary electron emission from the stub below so that
} the background appears dark behind the insects? My wires are glued to
} an aluminum stub with silver paint.
}
} Marie
}
} Dr. Marie E. Cantino
} Associate Professor of Physiology and Neurobiology
} Director, Electron Microscopy Laboratory
} University of Connecticut, Unit 3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}
}
} ==============================Original Headers==============================
} 4, 16 -- From marie.cantino-at-uconn.edu Tue Sep 29 16:37:58 2009
} 4, 16 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
} 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8TLbwPK031765
} 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 16:37:58 -0500
} 4, 16 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9])
} 4, 16 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n8TLbvGA003669
} 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 17:37:57 -0400
} 4, 16 -- Message-Id: {F764F989-B7AB-4442-82EF-E43A9A9626B5-at-uconn.edu}
} 4, 16 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
} 4, 16 -- To: microscopy-at-microscopy.com
} 4, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
} 4, 16 -- Content-Transfer-Encoding: 7bit
} 4, 16 -- Mime-Version: 1.0 (Apple Message framework v936)
} 4, 16 -- Subject: Dark backgrounds in SEM
} 4, 16 -- Date: Tue, 29 Sep 2009 17:37:57 -0400
} 4, 16 -- X-Mailer: Apple Mail (2.936)
} ==============================End of - Headers==============================
}
}

--



==============================Original Headers==============================
14, 24 -- From rstallcup-at-zyvex.com Tue Sep 29 17:35:45 2009
14, 24 -- Received: from thebe.zyvex.com (gw.zyvex.com [24.173.8.226])
14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8TMZigB010926
14, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 17:35:45 -0500
14, 24 -- Received: from [10.0.0.142] ([10.0.0.142])
14, 24 -- (authenticated bits=0)
14, 24 -- by thebe.zyvex.com (8.13.1/8.13.1) with ESMTP id n8TMZiQX018043
14, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
14, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 17:35:44 -0500
14, 24 -- Message-ID: {4AC28BC2.5080600-at-zyvex.com}
14, 24 -- Date: Tue, 29 Sep 2009 17:35:46 -0500
14, 24 -- From: Richard Stallcup {rstallcup-at-zyvex.com}
14, 24 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
14, 24 -- MIME-Version: 1.0
14, 24 -- To: Microscopy-at-microscopy.com
14, 24 -- Subject: Dark backgrounds in SEM
14, 24 -- References: {200909292150.n8TLo15M012270-at-ns.microscopy.com}
14, 24 -- In-Reply-To: {200909292150.n8TLo15M012270-at-ns.microscopy.com}
14, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 24 -- Content-Transfer-Encoding: 8bit
14, 24 -- X-Spam-Status: No, score=-1.4 required=5.0 tests=ALL_TRUSTED
14, 24 -- autolearn=disabled version=3.2.5
14, 24 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on thebe.zyvex.com
14, 24 -- Received-SPF: pass (thebe.zyvex.com: 10.0.0.142 is authenticated by a trusted mechanism)
==============================End of - Headers==============================




From: mmiralles-at-pi.ac.ae
Date: Wed, 30 Sep 2009 00:07:54 -0500
Subject: [Microscopy] Plasma Etcher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have been looking on a acquiring a new equipment for our SEM lab. One of which is a plasma etcher that was previously mentioned in my past query. My question is, will this be mainly applicable for cleaning surface contamination or it can also be used in the removal of metal coating (e.g. carbon or Au/Pd/Pt)?

Any recommendation for a reliable and simple unit?

Thank you so much.

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE
Tel :  +971 2 6075497 (Office)
       +971 2 6075539 (Lab)
Fax : +971 2 6075423
 



==============================Original Headers==============================
7, 27 -- From mmiralles-at-pi.ac.ae Wed Sep 30 00:07:54 2009
7, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8U57qFD005940
7, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 00:07:53 -0500
7, 27 -- X-IronPort-AV: E=Sophos;i="4.44,478,1249243200";
7, 27 -- d="scan'208";a="1596414"
7, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 27 -- by mx1.pi.ac.ae with ESMTP; 30 Sep 2009 08:42:26 +0400
7, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959);
7, 27 -- Wed, 30 Sep 2009 09:07:46 +0400
7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 27 -- Content-class: urn:content-classes:message
7, 27 -- MIME-Version: 1.0
7, 27 -- Content-Type: text/plain;
7, 27 -- charset="windows-1256"
7, 27 -- Subject: Plasma Etcher
7, 27 -- Date: Wed, 30 Sep 2009 09:07:46 +0400
7, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B07A19FA3-at-pi-exm.PI.AC.AE}
7, 27 -- X-MS-Has-Attach:
7, 27 -- X-MS-TNEF-Correlator:
7, 27 -- Thread-Topic: Plasma Etcher
7, 27 -- Thread-Index: AcpBi/LvxVFffuj+R/eTM2JJ47lYFQ==
7, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 27 -- To: {Microscopy-at-microscopy.com}
7, 27 -- X-OriginalArrivalTime: 30 Sep 2009 05:07:46.0873 (UTC) FILETIME=[F3207290:01CA418B]
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8U57qFD005940
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Wed, 30 Sep 2009 08:23:14 -0500
Subject: [Microscopy] Dark backgrounds in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You have had some good advice so far. One thing I have not seen yet is to mount the pin in such a manner as to tilt the sample and look at the structure over space. We examine thousands of pin mounted insects here and that is the preferred method. The background structure of the stage can still be visible however. To mitigate, my predecessor variously painted the stage with carbon paint. Effective, but to obtain a thick enough coat to stop beam the paint flakes off over time. Not really recommended. He also used carbon tape over everything. More effective, but sticks dust/dirt/debris which is then visible. Also difficult to remove. He also carbon coated glass and stuck back there. Very effective but difficult to make work. I ripped all that out and used Teflon foil. Worked beautifully but very hard to stick down. That is when I found graphite foil from Alfa Aesar. Easy to work with, cut and stick, washable and featureless, no off gassing or flaking etc...

We also use it as backgrounds for stubs and stage and since we use pin type stubs we raise them a bit and place folded sheets under stubs that swing around as the sample is tilted and rotated- always being in the background.

Stuff is not all that inexpensive, but I am on my second sheet in 10 years so no pain there.


Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891

-----Original Message-----
X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu]
Sent: Tuesday, September 29, 2009 5:41 PM
To: Whittaker, Scott

I am looking at insects mounted on wires in the SEM. Is there an easy
way to reduce secondary electron emission from the stub below so that
the background appears dark behind the insects? My wires are glued to
an aluminum stub with silver paint.

Marie

Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
4, 16 -- From marie.cantino-at-uconn.edu Tue Sep 29 16:37:58 2009
4, 16 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8TLbwPK031765
4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 16:37:58 -0500
4, 16 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9])
4, 16 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n8TLbvGA003669
4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2009 17:37:57 -0400
4, 16 -- Message-Id: {F764F989-B7AB-4442-82EF-E43A9A9626B5-at-uconn.edu}
4, 16 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
4, 16 -- To: microscopy-at-microscopy.com
4, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 16 -- Content-Transfer-Encoding: 7bit
4, 16 -- Mime-Version: 1.0 (Apple Message framework v936)
4, 16 -- Subject: Dark backgrounds in SEM
4, 16 -- Date: Tue, 29 Sep 2009 17:37:57 -0400
4, 16 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================


==============================Original Headers==============================
15, 29 -- From WHITTAKS-at-si.edu Wed Sep 30 08:23:14 2009
15, 29 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177])
15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8UDNEWO010531
15, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 08:23:14 -0500
15, 29 -- Received: from SI-MSEHUB-N01.US.SINET.SI.EDU (si-msehubi-01.us.sinet.si.edu [160.111.49.142])
15, 29 -- by si-mailout03.si.edu (Postfix) with ESMTP id D0FAF6762;
15, 29 -- Wed, 30 Sep 2009 09:23:13 -0400 (EDT)
15, 29 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::58ac:1cff:44b0:524e]) by
15, 29 -- SI-MSEHUB-N01.US.SINET.SI.EDU ([fe80::34ec:9394:533a:63ad%11]) with mapi;
15, 29 -- Wed, 30 Sep 2009 09:23:13 -0400
15, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
15, 29 -- To: "'marie.cantino-at-uconn.edu'" {marie.cantino-at-uconn.edu}
15, 29 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
15, 29 -- Date: Wed, 30 Sep 2009 09:23:11 -0400
15, 29 -- Subject: RE: [Microscopy] Dark backgrounds in SEM
15, 29 -- Thread-Topic: [Microscopy] Dark backgrounds in SEM
15, 29 -- Thread-Index: AcpBTX5STKxpuijIQoKWDqAfwLh6zAAghrCQ
15, 29 -- Message-ID: {038C04650A93A14381E944A473695F618C6B0748BD-at-SI-MSEV03.US.SINET.SI.EDU}
15, 29 -- References: {200909292140.n8TLeefB001545-at-ns.microscopy.com}
15, 29 -- In-Reply-To: {200909292140.n8TLeefB001545-at-ns.microscopy.com}
15, 29 -- Accept-Language: en-US
15, 29 -- Content-Language: en-US
15, 29 -- X-MS-Has-Attach:
15, 29 -- X-MS-TNEF-Correlator:
15, 29 -- acceptlanguage: en-US
15, 29 -- Content-Type: text/plain; charset="iso-8859-1"
15, 29 -- MIME-Version: 1.0
15, 29 -- Content-Transfer-Encoding: 8bit
15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8UDNEWO010531
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Wed, 30 Sep 2009 10:39:49 -0500
Subject: [Microscopy] Plasma Etcher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Melina,

A plasma etcher or a plasma cleaner are very similar in their application.
Generally, they are designed to remove hydrocarbons with an oxygen plasma.
The etcher is used at a higher power than the cleaner and its purpose is the
removal of bulk polymeric material. They can also be used to remove certain
materials by a reactive ion mechanism. With that the gas forms a volatile
species with the surface materials and takes them away. An example is
CF4+O2 gas mix with Si or SiO2. SiF4 and CO2 are formed which are pumped
away. There are some gases that will work with a few metals. Cl2 and BCl3
are used with Al. However, I don't think that there are any reactive etches
for Au/Pt/Pd coatings. To quote Randy Newman, "I could be wrong, but I
don't think so." Anyway, one way to remove those is physical sputtering in
an RIE system (Reactive Ion Etch). SBT sells such an instrument, but it
would be an expensive option for your application. In this type of system,
the sample sits on the antenna of the capacitively coupled antenna which
develops a negative voltage. If the power of the plasma is raised high
enough, the voltage on the antenna is high enough to cause the ions
extracted from the plasma to sputter material away. Reactive gases are also
used to enhance removal rates and hence the name. RIE systems are used to
etch samples anisotropically (directional) while plasma etchers and cleaners
will etch isotropically (omnidirectional).

Recently, we have developed Plasma Trimming™ tools for our Plasma Cleaner
which uses this principle for cleaning the damage from FIB samples. It
could also be used in your application to remove the coatings from your
samples. Basically, we designed a clamping plate for the antenna of the
plasma cleaner that holds the sample upside down on the antenna of the
plasma cleaner. By running the plasma cleaner at a much higher power than
is used for cleaning, the ions will sputter the surface in the same way as
it does in the RIE. You control the energy of the ions by controlling the
dc bias on the antenna. Increasing the power will increase the energy and
decreasing the pressure will increase the energy. For your application,
unlike the use of this system for FIB samples, the ions would strike the
surface of your sample at normal incidence. To avoid surface damage, you
would have to balance the energy of the system with the desired removal
rate.

If you would like more information on this process, please contact me
offline.

Disclaimer: South Bay Technology manufactures and sells the RIE and plasma
cleaner instrumentation.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae]
Sent: Tuesday, September 29, 2009 10:17 PM
To: swalck-at-southbaytech.com

Hi all,

I have been looking on a acquiring a new equipment for our SEM lab. One of
which is a plasma etcher that was previously mentioned in my past query. My
question is, will this be mainly applicable for cleaning surface
contamination or it can also be used in the removal of metal coating (e.g.
carbon or Au/Pd/Pt)?

Any recommendation for a reliable and simple unit?

Thank you so much.

Melina Miralles
Petroleum Geosciences Lab Technician
The Petroleum Institute
Abu Dhabi, UAE
Tel :  +971 2 6075497 (Office)
       +971 2 6075539 (Lab)
Fax : +971 2 6075423
 



==============================Original Headers==============================
7, 27 -- From mmiralles-at-pi.ac.ae Wed Sep 30 00:07:54 2009
7, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n8U57qFD005940
7, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 00:07:53
-0500
7, 27 -- X-IronPort-AV: E=Sophos;i="4.44,478,1249243200";
7, 27 -- d="scan'208";a="1596414"
7, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11])
7, 27 -- by mx1.pi.ac.ae with ESMTP; 30 Sep 2009 08:42:26 +0400
7, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE
with Microsoft SMTPSVC(6.0.3790.3959);
7, 27 -- Wed, 30 Sep 2009 09:07:46 +0400
7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 27 -- Content-class: urn:content-classes:message
7, 27 -- MIME-Version: 1.0
7, 27 -- Content-Type: text/plain;
7, 27 -- charset="windows-1256"
7, 27 -- Subject: Plasma Etcher
7, 27 -- Date: Wed, 30 Sep 2009 09:07:46 +0400
7, 27 -- Message-ID:
{D5603421C6303A46883F87E7968F285B07A19FA3-at-pi-exm.PI.AC.AE}
7, 27 -- X-MS-Has-Attach:
7, 27 -- X-MS-TNEF-Correlator:
7, 27 -- Thread-Topic: Plasma Etcher
7, 27 -- Thread-Index: AcpBi/LvxVFffuj+R/eTM2JJ47lYFQ==
7, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae}
7, 27 -- To: {Microscopy-at-microscopy.com}
7, 27 -- X-OriginalArrivalTime: 30 Sep 2009 05:07:46.0873 (UTC)
FILETIME=[F3207290:01CA418B]
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n8U57qFD005940
==============================End of - Headers==============================




==============================Original Headers==============================
22, 24 -- From swalck-at-southbaytech.com Wed Sep 30 10:39:48 2009
22, 24 -- Received: from relay02.pair.com (relay02.pair.com [209.68.5.16])
22, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n8UFdmsj030754
22, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 10:39:48 -0500
22, 24 -- Received: (qmail 65689 invoked from network); 30 Sep 2009 15:39:47 -0000
22, 24 -- Received: from 99.154.21.201 (HELO SWALCKD1) (99.154.21.201)
22, 24 -- by relay02.pair.com with SMTP; 30 Sep 2009 15:39:47 -0000
22, 24 -- X-pair-Authenticated: 99.154.21.201
22, 24 -- From: "Scott Walck" {swalck-at-southbaytech.com}
22, 24 -- To: {Microscopy-at-microscopy.com}
22, 24 -- Cc: {mmiralles-at-pi.ac.ae}
22, 24 -- References: {200909300516.n8U5GsOT020124-at-ns.microscopy.com}
22, 24 -- In-Reply-To: {200909300516.n8U5GsOT020124-at-ns.microscopy.com}
22, 24 -- Subject: RE: [Microscopy] Plasma Etcher
22, 24 -- Date: Wed, 30 Sep 2009 08:41:58 -0700
22, 24 -- Message-ID: {00c101ca41e4$8be881e0$a3b985a0$-at-com}
22, 24 -- MIME-Version: 1.0
22, 24 -- Content-Type: text/plain;
22, 24 -- charset="iso-8859-1"
22, 24 -- X-Mailer: Microsoft Office Outlook 12.0
22, 24 -- Thread-Index: AcpBjTnVJukFyiy7S6OQoHVJB66rQgAUt3kA
22, 24 -- Content-Language: en-us
22, 24 -- Content-Transfer-Encoding: 8bit
22, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n8UFdmsj030754
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Wed, 30 Sep 2009 16:19:31 -0500
Subject: [Microscopy] mouse embryo TEM protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I need some feedback for a protocol to process E12.5 mouse embryos for
TEM. The first batch was in fix too long and was unusable. The critical
factors I am asking about are fixative concentrations, times and buffer
molarity. No speculation please, only results based on actual successful
experiments.

The provisional protocol is below:
Thanks,
Larry

Protocol for mouse embryo electron microscopy October 1, 2009

Primary fixation:

4% formaldehyde, freshly made or EM grade, methanol free
3% EM grade glutaraldehyde
O.1M PO4 buffer, pH 7.2
Room temperature, immersion for no more than two hours, slice embryos in
half with sharp razor blade after 10 or 15 minutes in fix

Rinse:
01.M PO4, pH 7.2 3X 10 minutes

1% OsO4 in 0.1M PO4 2 hours—tissue should turn black

Rinse:
01.M PO4, pH 7.2 3X 5 minutes

1X DH2O 5 minutes

enblock stain:
2% uranyl acetate in H2O one hour

Dehydrate:

35% ethanol 10 minutes
70% ethanol 10 minutes
95% ethanol 10 minutes
100% ethanol 10 minutes X3

propylene oxide 2X 10 minutes

epoxy resin : propylene oxide 2X 1:1 (EMBed812 or similar resin) two
hours or overnight

fresh resin two—four hours

fresh resin in embedding mold

polymerize 60 degrees C 48 hours

==============================Original Headers==============================
17, 30 -- From Larry.Ackerman-at-ucsf.edu Wed Sep 30 16:19:31 2009
17, 30 -- Received: from bcuda4.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ULJUHC030296
17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:19:31 -0500
17, 30 -- X-ASG-Debug-ID: 1254345569-0aaa01c90000-1DjkGe
17, 30 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
17, 30 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
17, 30 -- by bcuda4.ucsf.edu (Spam & Virus Firewall) with ESMTP id 2471F10D5A84
17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700 (PDT)
17, 30 -- Received: from EXHT03.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda4.ucsf.edu with ESMTP id 0iszrBkl7IjZZnmv for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700 (PDT)
17, 30 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
17, 30 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
17, 30 -- Received: from Ralston-Lab-iMac.local (128.218.239.124) by EXHT03.net.ucsf.edu
17, 30 -- (64.54.247.220) with Microsoft SMTP Server id 8.1.393.1; Wed, 30 Sep 2009
17, 30 -- 14:19:29 -0700
17, 30 -- Message-ID: {4AC3CB56.7050705-at-ucsf.edu}
17, 30 -- Date: Wed, 30 Sep 2009 14:19:18 -0700
17, 30 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
17, 30 -- Reply-To: larry.ackerman-at-ucsf.edu
17, 30 -- Organization: UCSF, NeuroAnatomy
17, 30 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
17, 30 -- MIME-Version: 1.0
17, 30 -- To: {Microscopy-at-microscopy.com}
17, 30 -- X-ASG-Orig-Subj: mouse embryo TEM protocol
17, 30 -- Subject: mouse embryo TEM protocol
17, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
17, 30 -- Content-Transfer-Encoding: 8bit
17, 30 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
17, 30 -- X-Barracuda-Start-Time: 1254345570
17, 30 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Wed, 30 Sep 2009 16:51:45 -0500
Subject: [Microscopy] Re: mouse embryo TEM protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was not quite clear. The failed experiment tissue was in fix for 4 or
5 days before I received it. The lengthy fix has been okay with other
adult tissues but not this sample. The first clue was that the tissue
did not turn black in osmium just pale brown. The sections had extremely
low contrast with no membranes visible. This project is looking at
cellular junctional complexes.

Larry

Markus F. Meyenhofer wrote:
} 2 hours in Fixative is too long?? I don't (and many others) think so!
} What does "unusable" mean?
} Please explain.
} Regards,
} Markus F. Meyenhofer
} Microscopy Labs
} Red Bank, NJ
}
}
} ----- Original Message -----
} From: {larry.ackerman-at-ucsf.edu}
} To: {micro-at-superlink.net}
} Sent: Wednesday, September 30, 2009 5:19 PM
} Subject: [Microscopy] mouse embryo TEM protocol
}
}
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi,
} } I need some feedback for a protocol to process E12.5 mouse embryos for
} } TEM. The first batch was in fix too long and was unusable. The critical
} } factors I am asking about are fixative concentrations, times and buffer
} } molarity. No speculation please, only results based on actual successful
} } experiments.
} }
} } The provisional protocol is below:
} } Thanks,
} } Larry
} }
} } Protocol for mouse embryo electron microscopy October 1, 2009
} }
} } Primary fixation:
} }
} } 4% formaldehyde, freshly made or EM grade, methanol free
} } 3% EM grade glutaraldehyde
} } O.1M PO4 buffer, pH 7.2
} } Room temperature, immersion for no more than two hours, slice embryos in
} } half with sharp razor blade after 10 or 15 minutes in fix
} }
} } Rinse:
} } 01.M PO4, pH 7.2 3X 10 minutes
} }
} } 1% OsO4 in 0.1M PO4 2 hours-tissue should turn black
} }
} } Rinse:
} } 01.M PO4, pH 7.2 3X 5 minutes
} }
} } 1X DH2O 5 minutes
} }
} } enblock stain:
} } 2% uranyl acetate in H2O one hour
} }
} } Dehydrate:
} }
} } 35% ethanol 10 minutes
} } 70% ethanol 10 minutes
} } 95% ethanol 10 minutes
} } 100% ethanol 10 minutes X3
} }
} } propylene oxide 2X 10 minutes
} }
} } epoxy resin : propylene oxide 2X 1:1 (EMBed812 or similar resin) two
} } hours or overnight
} }
} } fresh resin two-four hours
} }
} } fresh resin in embedding mold
} }
} } polymerize 60 degrees C 48 hours
} }
} } ==============================Original
} } Headers==============================
} } 17, 30 -- From Larry.Ackerman-at-ucsf.edu Wed Sep 30 16:19:31 2009
} } 17, 30 -- Received: from bcuda4.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
} } 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n8ULJUHC030296
} } 17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:19:31 -0500
} } 17, 30 -- X-ASG-Debug-ID: 1254345569-0aaa01c90000-1DjkGe
} } 17, 30 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
} } 17, 30 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
} } 17, 30 -- by bcuda4.ucsf.edu (Spam & Virus Firewall) with ESMTP id
} } 2471F10D5A84
} } 17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700
} } (PDT)
} } 17, 30 -- Received: from EXHT03.net.ucsf.edu (mx.ucsf.edu [64.54.247.193])
} } by bcuda4.ucsf.edu with ESMTP id 0iszrBkl7IjZZnmv for
} } {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700 (PDT)
} } 17, 30 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
} } 17, 30 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
} } 17, 30 -- Received: from Ralston-Lab-iMac.local (128.218.239.124) by
} } EXHT03.net.ucsf.edu
} } 17, 30 -- (64.54.247.220) with Microsoft SMTP Server id 8.1.393.1; Wed,
} } 30 Sep 2009
} } 17, 30 -- 14:19:29 -0700
} } 17, 30 -- Message-ID: {4AC3CB56.7050705-at-ucsf.edu}
} } 17, 30 -- Date: Wed, 30 Sep 2009 14:19:18 -0700
} } 17, 30 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
} } 17, 30 -- Reply-To: larry.ackerman-at-ucsf.edu
} } 17, 30 -- Organization: UCSF, NeuroAnatomy
} } 17, 30 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
} } 17, 30 -- MIME-Version: 1.0
} } 17, 30 -- To: {Microscopy-at-microscopy.com}
} } 17, 30 -- X-ASG-Orig-Subj: mouse embryo TEM protocol
} } 17, 30 -- Subject: mouse embryo TEM protocol
} } 17, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
} } 17, 30 -- Content-Transfer-Encoding: 8bit
} } 17, 30 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
} } 17, 30 -- X-Barracuda-Start-Time: 1254345570
} } 17, 30 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at
} } ucsf.edu
} } ==============================End of -
} } Headers==============================
} }
}
} .
}

==============================Original Headers==============================
3, 32 -- From Larry.Ackerman-at-ucsf.edu Wed Sep 30 16:51:45 2009
3, 32 -- Received: from bcuda4.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ULpihJ014028
3, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:51:45 -0500
3, 32 -- X-ASG-Debug-ID: 1254347504-0aa5023e0000-1DjkGe
3, 32 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
3, 32 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
3, 32 -- by bcuda4.ucsf.edu (Spam & Virus Firewall) with ESMTP id 59BD310D67A3
3, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:51:44 -0700 (PDT)
3, 32 -- Received: from EXHT03.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda4.ucsf.edu with ESMTP id RrXIKTU6ZQnCd1Pi for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:51:44 -0700 (PDT)
3, 32 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
3, 32 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
3, 32 -- Received: from Ralston-Lab-iMac.local (128.218.239.124) by EXHT03.net.ucsf.edu
3, 32 -- (64.54.247.220) with Microsoft SMTP Server id 8.1.393.1; Wed, 30 Sep 2009
3, 32 -- 14:51:42 -0700
3, 32 -- Message-ID: {4AC3D2E2.8010507-at-ucsf.edu}
3, 32 -- Date: Wed, 30 Sep 2009 14:51:30 -0700
3, 32 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
3, 32 -- Reply-To: larry.ackerman-at-ucsf.edu
3, 32 -- Organization: UCSF, NeuroAnatomy
3, 32 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
3, 32 -- MIME-Version: 1.0
3, 32 -- To: {Microscopy-at-microscopy.com}
3, 32 -- X-ASG-Orig-Subj: Re: [Microscopy] mouse embryo TEM protocol
3, 32 -- Subject: Re: [Microscopy] mouse embryo TEM protocol
3, 32 -- References: {200909302119.n8ULJi9u030572-at-ns.microscopy.com} {B406ADFEE90C42BF8E7ADF0F8C166187-at-DJ4VDH31}
3, 32 -- In-Reply-To: {B406ADFEE90C42BF8E7ADF0F8C166187-at-DJ4VDH31}
3, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
3, 32 -- Content-Transfer-Encoding: 7bit
3, 32 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
3, 32 -- X-Barracuda-Start-Time: 1254347504
3, 32 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================




From: wieman-at-matsys.com
Date: Wed, 30 Sep 2009 16:56:39 -0500
Subject: [Microscopy] viaWWW: Seeking an SEM Keyboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wieman-at-matsys.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wieman-at-matsys.com
Name: Thomas Wieman

Title-Subject: [Filtered] Seeking an SEM Keyboard

Question: We have a JOEL J5m6100 SEM currently at our company, We did
not receive a keyboard with this machine, and it is currently not
wired. We are looking to start using it in the next few weeks, and
have been unsuccessful so far in finding a keyboard for it. Can
anyone help me with this issue? I am looking for a website, or
company that may have a keyboard that can be used with this machine.
Thanks you for your help.

Login Host: 69.244.232.212
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Wed Sep 30 16:56:39 2009
5, 11 -- Received: from [172.22.2.6] ([130.202.238.72])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ULuafX021548
5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:56:38 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240807c6e98480768f-at-[172.22.2.6]}
5, 11 -- Date: Thu, 1 Oct 2009 06:56:36 +0900
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: wieman-at-matsys.com (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: Seeking an SEM Keyboard
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Wed, 30 Sep 2009 22:05:13 -0500
Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

We have some users who wish to examine biological sections mounted on nickel
grids in our JEOL 2100 cryo TEM (200kV). These will be examined with either
a JEOL standard quick exchange holder or a JEOL non-analytical double tilt
holder. In both cases, the specimens are held in place by a retainer. This
is screwed down on one side only of the specimen. Retaining force is then
applied to the specimen via the residual springiness of the retainer.

Question 1: Is there any realistic possibility that a nickel grid may get
pulled out of the holder by the field and get stuck on the polepiece with
this type arrangement?

Question 2: The biological folk are using nickel grids for immunogold work.
I'm not a biologist, but it seems that using a ferromagnetic material for a
support grid, is best avoided wherever possible. Are there other types of
grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
immuno-gold labeling?

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com





==============================Original Headers==============================
14, 27 -- From david.mitchell-at-emu.usyd.edu.au Wed Sep 30 22:05:12 2009
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9135Bdc024824
14, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 22:05:12 -0500
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1])
14, 27 -- by localhost (Postfix) with SMTP id 09349103899
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:05:09 +1000 (EST)
14, 27 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au (ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
14, 27 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id EA54F1039B1
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:05:08 +1000 (EST)
14, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by ex-bri-pro-01.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
14, 27 -- Thu, 1 Oct 2009 13:05:08 +1000
14, 27 -- Received: from 172.17.185.135 ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ;
14, 27 -- Thu, 1 Oct 2009 03:05:08 +0000
14, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
14, 27 -- Date: Thu, 01 Oct 2009 13:05:07 +1000
14, 27 -- Subject: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
14, 27 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
14, 27 -- To: {Microscopy-at-microscopy.com}
14, 27 -- Message-ID: {C6EA5983.1983%david.mitchell-at-emu.usyd.edu.au}
14, 27 -- Thread-Topic: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
14, 27 -- Thread-Index: AcpCQ/q2OUQPRK43Ed6dogAjMrpsqg==
14, 27 -- Mime-version: 1.0
14, 27 -- Content-type: text/plain;
14, 27 -- charset="US-ASCII"
14, 27 -- Content-transfer-encoding: 7bit
14, 27 -- X-OriginalArrivalTime: 01 Oct 2009 03:05:08.0655 (UTC) FILETIME=[FBB33BF0:01CA4243]
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 1 Oct 2009 09:56:51 -0500
Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have never heard of any problems with grids being pulled out of the holder by the field in the TEM. The likeliest problems are grids clinging to forceps and occasional weirdness with image (distortions, etc.) with severely magnetized grids.

If you just don't want to use nickel, gold grids will work well for immuno work, but they're more expensive. Just don't try to label with ultra-small gold probes and then use gold-enhancement to increase their visibility after labeling. Obviously, the grid will be "enhanced" right along with the gold nanos.

Finally, I have seen people use plain, old copper grids for labeling with success. The idea that copper grids are not to be used for immunolabeling is embedded in the EM psyche, but I have wondered many times how important that really is. Never hurts to try alternatives, just for fun if nothing else.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: david.mitchell-at-emu.usyd.edu.au [mailto:david.mitchell-at-emu.usyd.edu.au]
Sent: Wednesday, September 30, 2009 10:07 PM
To: Tindall, Randy D.

Dear All

We have some users who wish to examine biological sections mounted on nickel
grids in our JEOL 2100 cryo TEM (200kV). These will be examined with either
a JEOL standard quick exchange holder or a JEOL non-analytical double tilt
holder. In both cases, the specimens are held in place by a retainer. This
is screwed down on one side only of the specimen. Retaining force is then
applied to the specimen via the residual springiness of the retainer.

Question 1: Is there any realistic possibility that a nickel grid may get
pulled out of the holder by the field and get stuck on the polepiece with
this type arrangement?

Question 2: The biological folk are using nickel grids for immunogold work.
I'm not a biologist, but it seems that using a ferromagnetic material for a
support grid, is best avoided wherever possible. Are there other types of
grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
immuno-gold labeling?

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com





==============================Original Headers==============================
14, 27 -- From david.mitchell-at-emu.usyd.edu.au Wed Sep 30 22:05:12 2009
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9135Bdc024824
14, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 22:05:12 -0500
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1])
14, 27 -- by localhost (Postfix) with SMTP id 09349103899
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:05:09 +1000 (EST)
14, 27 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au (ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
14, 27 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id EA54F1039B1
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:05:08 +1000 (EST)
14, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by ex-bri-pro-01.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
14, 27 -- Thu, 1 Oct 2009 13:05:08 +1000
14, 27 -- Received: from 172.17.185.135 ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ;
14, 27 -- Thu, 1 Oct 2009 03:05:08 +0000
14, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
14, 27 -- Date: Thu, 01 Oct 2009 13:05:07 +1000
14, 27 -- Subject: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
14, 27 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
14, 27 -- To: {Microscopy-at-microscopy.com}
14, 27 -- Message-ID: {C6EA5983.1983%david.mitchell-at-emu.usyd.edu.au}
14, 27 -- Thread-Topic: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
14, 27 -- Thread-Index: AcpCQ/q2OUQPRK43Ed6dogAjMrpsqg==
14, 27 -- Mime-version: 1.0
14, 27 -- Content-type: text/plain;
14, 27 -- charset="US-ASCII"
14, 27 -- Content-transfer-encoding: 7bit
14, 27 -- X-OriginalArrivalTime: 01 Oct 2009 03:05:08.0655 (UTC) FILETIME=[FBB33BF0:01CA4243]
==============================End of - Headers==============================


==============================Original Headers==============================
27, 35 -- From TindallR-at-missouri.edu Thu Oct 1 09:56:50 2009
27, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
27, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91Euo3K018155
27, 35 -- for {microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 09:56:50 -0500
27, 35 -- X-IronPort-Anti-Spam-Filtered: true
27, 35 -- X-IronPort-Anti-Spam-Result: ApoEAJNgxErRauUp/2dsb2JhbADILgEJhk6IS4JLgV4EgU+IOw
27, 35 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
27, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 01 Oct 2009 09:55:52 -0500
27, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
27, 35 -- Thu, 1 Oct 2009 09:55:12 -0500
27, 35 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
27, 35 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 1 Oct 2009
27, 35 -- 09:55:11 -0500
27, 35 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
27, 35 -- To: "'david.mitchell-at-emu.usyd.edu.au'" {david.mitchell-at-emu.usyd.edu.au}
27, 35 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
27, 35 -- Date: Thu, 1 Oct 2009 09:55:10 -0500
27, 35 -- Subject: RE: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold
27, 35 -- labeling
27, 35 -- Thread-Topic: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold
27, 35 -- labeling
27, 35 -- Thread-Index: AcpCRC4NJdK6XQS8SBiUTFABEvWsnAAYasow
27, 35 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129877C93A-at-UM-EMAIL06.um.umsystem.edu}
27, 35 -- References: {200910010306.n9136VXm026414-at-ns.microscopy.com}
27, 35 -- In-Reply-To: {200910010306.n9136VXm026414-at-ns.microscopy.com}
27, 35 -- Accept-Language: en-US
27, 35 -- Content-Language: en-US
27, 35 -- X-MS-Has-Attach:
27, 35 -- X-MS-TNEF-Correlator:
27, 35 -- acceptlanguage: en-US
27, 35 -- Content-Type: text/plain; charset="us-ascii"
27, 35 -- MIME-Version: 1.0
27, 35 -- X-OriginalArrivalTime: 01 Oct 2009 14:55:12.0108 (UTC) FILETIME=[2D5642C0:01CA42A7]
27, 35 -- Content-Transfer-Encoding: 8bit
27, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n91Euo3K018155
==============================End of - Headers==============================




From: thoward-at-unm.edu
Date: Thu, 1 Oct 2009 10:16:00 -0500
Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can address the "Why we don't use Cu grids for IEM"
question, having experienced the problem. It only applies
if you use Cu grids and Tris buffers for the labeling -
the Tris etches the Cu and you wind up with lovely blue
drops of Cu-laden buffer & severe debris on the sections!
Cu grids with phosphate-based buffers are fine for IEM.
I've never tried anything other than Tris and phosphate
for IEM for so I don't know what other buffer salts will
do.

I've never had problems (other than the expense & tendency
to fold up into into tacos) with Au grids. Au-clad Cu
grids have been iffy; they are cheaper than plain Au &
definitely sturdier, but the cladding on the batches I've
tried has not always been complete - I lost part of an
experiment to the Tris-Cu curse - so only use those with
phosphate buffers.

Tamara

On Thu, 1 Oct 2009 09:58:23 -0500
TindallR-at-missouri.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I have never heard of any problems with grids being
} pulled out of the holder by the field in the TEM. The
} likeliest problems are grids clinging to forceps and
} occasional weirdness with image (distortions, etc.) with
} severely magnetized grids.
}
} If you just don't want to use nickel, gold grids will
} work well for immuno work, but they're more expensive.
} Just don't try to label with ultra-small gold probes and
} then use gold-enhancement to increase their visibility
} after labeling. Obviously, the grid will be "enhanced"
} right along with the gold nanos.
}
} Finally, I have seen people use plain, old copper grids
} for labeling with success. The idea that copper grids
} are not to be used for immunolabeling is embedded in the
} EM psyche, but I have wondered many times how important
} that really is. Never hurts to try alternatives, just
} for fun if nothing else.
}
} Cheers,
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
} -----Original Message-----
} X-from: david.mitchell-at-emu.usyd.edu.au
} [mailto:david.mitchell-at-emu.usyd.edu.au]
} Sent: Wednesday, September 30, 2009 10:07 PM
} To: Tindall, Randy D.
} Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for
} immuno-gold labeling
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All
}
} We have some users who wish to examine biological
} sections mounted on nickel
} grids in our JEOL 2100 cryo TEM (200kV). These will be
} examined with either
} a JEOL standard quick exchange holder or a JEOL
} non-analytical double tilt
} holder. In both cases, the specimens are held in place
} by a retainer. This
} is screwed down on one side only of the specimen.
} Retaining force is then
} applied to the specimen via the residual springiness of
} the retainer.
}
} Question 1: Is there any realistic possibility that a
} nickel grid may get
} pulled out of the holder by the field and get stuck on
} the polepiece with
} this type arrangement?
}
} Question 2: The biological folk are using nickel grids
} for immunogold work.
} I'm not a biologist, but it seems that using a
} ferromagnetic material for a
} support grid, is best avoided wherever possible. Are
} there other types of
} grid (non-ferromagnetic - Al, Mo, nylon etc) which are
} compatible with
} immuno-gold labeling?
}
} Thanks and regards,
}
} Dave Mitchell
}
}
} Dr David Mitchell
} Senior Microscopist, Transmission Electron Microscopy
}
} Contact:
} PH +61 2 9036 7633
} FAX +61 2 9351 7682
} David.mitchell-at-emu.usyd.edu.au
}
} Address:
} Electron Microscope Unit
} Australian Key Centre for Microscopy and Microanalysis
} Australian Microscopy & Microanalysis Research Facility
} (AMMRF)
} Madsen Building F09, Room 111A
} The University of Sydney
} NSW 2006, Australia
} www.emu.usyd.edu.au
} www.ammrf.org.au
}
} www.dmscripting.com
}
}
}
}
}
} ==============================Original
} Headers==============================
} 14, 27 -- From david.mitchell-at-emu.usyd.edu.au Wed Sep 30
} 22:05:12 2009
} 14, 27 -- Received: from baghdad.ucc.usyd.edu.au
} (baghdad.ucc.usyd.edu.au [129.78.64.146])
} 14, 27 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n9135Bdc024824
} 14, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep
} 2009 22:05:12 -0500
} 14, 27 -- Received: from baghdad.ucc.usyd.edu.au
} (localhost [127.0.0.1])
} 14, 27 -- by localhost (Postfix) with SMTP id
} 09349103899
} 14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct
} 2009 13:05:09 +1000 (EST)
} 14, 27 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au
} (ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
} 14, 27 -- by baghdad.ucc.usyd.edu.au (Postfix) with
} ESMTP id EA54F1039B1
} 14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct
} 2009 13:05:08 +1000 (EST)
} 14, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au
} ([172.17.63.2]) by ex-bri-pro-01.mcs.usyd.edu.au with
} Microsoft SMTPSVC(6.0.3790.1830);
} 14, 27 -- Thu, 1 Oct 2009 13:05:08 +1000
} 14, 27 -- Received: from 172.17.185.135
} ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au
} ([172.17.63.4]) via Exchange Front-End Server
} www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft
} Exchange Server HTTP-DAV ;
} 14, 27 -- Thu, 1 Oct 2009 03:05:08 +0000
} 14, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
} 14, 27 -- Date: Thu, 01 Oct 2009 13:05:07 +1000
} 14, 27 -- Subject: TEM: Nickel Grids in 200kV TEM for
} immuno-gold labeling
} 14, 27 -- From: David Mitchell
} {david.mitchell-at-emu.usyd.edu.au}
} 14, 27 -- To: {Microscopy-at-microscopy.com}
} 14, 27 -- Message-ID:
} {C6EA5983.1983%david.mitchell-at-emu.usyd.edu.au}
} 14, 27 -- Thread-Topic: TEM: Nickel Grids in 200kV TEM
} for immuno-gold labeling
} 14, 27 -- Thread-Index: AcpCQ/q2OUQPRK43Ed6dogAjMrpsqg==
} 14, 27 -- Mime-version: 1.0
} 14, 27 -- Content-type: text/plain;
} 14, 27 -- charset="US-ASCII"
} 14, 27 -- Content-transfer-encoding: 7bit
} 14, 27 -- X-OriginalArrivalTime: 01 Oct 2009
} 03:05:08.0655 (UTC) FILETIME=[FBB33BF0:01CA4243]
} ==============================End of -
} Headers==============================
}
}
} ==============================Original
} Headers==============================
} 27, 35 -- From TindallR-at-missouri.edu Thu Oct 1 09:56:50
} 2009
} 27, 35 -- Received: from
} mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 27, 35 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id n91Euo3K018155
} 27, 35 -- for {microscopy-at-microscopy.com} ; Thu, 1 Oct
} 2009 09:56:50 -0500
} 27, 35 -- X-IronPort-Anti-Spam-Filtered: true
} 27, 35 -- X-IronPort-Anti-Spam-Result:
} ApoEAJNgxErRauUp/2dsb2JhbADILgEJhk6IS4JLgV4EgU+IOw
} 27, 35 -- Received: from unknown (HELO
} um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
} 27, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with
} ESMTP; 01 Oct 2009 09:55:52 -0500
} 27, 35 -- Received: from UM-THUB01.um.umsystem.edu
} ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with
} Microsoft SMTPSVC(6.0.3790.3959);
} 27, 35 -- Thu, 1 Oct 2009 09:55:12 -0500
} 27, 35 -- Received: from um-email06.um.umsystem.edu
} ([169.254.1.222]) by
} 27, 35 -- UM-THUB01.um.umsystem.edu ([209.106.230.181])
} with mapi; Thu, 1 Oct 2009
} 27, 35 -- 09:55:11 -0500
} 27, 35 -- From: "Tindall, Randy D."
} {TindallR-at-missouri.edu}
} 27, 35 -- To: "'david.mitchell-at-emu.usyd.edu.au'"
} {david.mitchell-at-emu.usyd.edu.au}
} 27, 35 -- CC: "'microscopy-at-microscopy.com'"
} {microscopy-at-microscopy.com}
} 27, 35 -- Date: Thu, 1 Oct 2009 09:55:10 -0500
} 27, 35 -- Subject: RE: [Microscopy] TEM: Nickel Grids in
} 200kV TEM for immuno-gold
} 27, 35 -- labeling
} 27, 35 -- Thread-Topic: [Microscopy] TEM: Nickel Grids
} in 200kV TEM for immuno-gold
} 27, 35 -- labeling
} 27, 35 -- Thread-Index:
} AcpCRC4NJdK6XQS8SBiUTFABEvWsnAAYasow
} 27, 35 -- Message-ID:
} {9422E68616A7C648A281C0B5CD22A4B8129877C93A-at-UM-EMAIL06.um.umsystem.edu}
} 27, 35 -- References:
} {200910010306.n9136VXm026414-at-ns.microscopy.com}
} 27, 35 -- In-Reply-To:
} {200910010306.n9136VXm026414-at-ns.microscopy.com}
} 27, 35 -- Accept-Language: en-US
} 27, 35 -- Content-Language: en-US
} 27, 35 -- X-MS-Has-Attach:
} 27, 35 -- X-MS-TNEF-Correlator:
} 27, 35 -- acceptlanguage: en-US
} 27, 35 -- Content-Type: text/plain; charset="us-ascii"
} 27, 35 -- MIME-Version: 1.0
} 27, 35 -- X-OriginalArrivalTime: 01 Oct 2009
} 14:55:12.0108 (UTC) FILETIME=[2D5642C0:01CA42A7]
} 27, 35 -- Content-Transfer-Encoding: 8bit
} 27, 35 -- X-MIME-Autoconverted: from quoted-printable to
} 8bit by ns.microscopy.com id n91Euo3K018155
} ==============================End of -
} Headers==============================

***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************


==============================Original Headers==============================
6, 37 -- From thoward-at-unm.edu Thu Oct 1 10:15:59 2009
6, 37 -- Received: from unm.edu (arianrhod.unm.edu [129.24.170.101])
6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91FFxKi001720
6, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 10:15:59 -0500
6, 37 -- Received: from [129.24.208.82] (HELO clio.unm.edu)
6, 37 -- by arianrhod.unm.edu (CommuniGate Pro SMTP 5.2.15)
6, 37 -- with ESMTP id 6960483; Thu, 01 Oct 2009 09:15:58 -0600
6, 37 -- Received: from clio.unm.edu (localhost.localdomain [127.0.0.1])
6, 37 -- by localhost (Postfix) with SMTP id 857F85EC00B;
6, 37 -- Thu, 1 Oct 2009 09:15:58 -0600 (MDT)
6, 37 -- Received: from unm.edu (nusakan.unm.edu [129.24.170.106])
6, 37 -- by clio.unm.edu (Postfix) with ESMTP id 119705EC001;
6, 37 -- Thu, 1 Oct 2009 09:15:58 -0600 (MDT)
6, 37 -- Received: by nusakan.unm.edu (CommuniGate Pro PIPE 5.2.15)
6, 37 -- with PIPE id 6701135; Thu, 01 Oct 2009 09:15:58 -0600
6, 37 -- X-PMX-Host: nusakan - CGF3
6, 37 -- Received: from [64.234.168.129] (account thoward-at-unm.edu)
6, 37 -- by ceaster.unm.edu (CommuniGate Pro WEBUSER 5.2.15)
6, 37 -- with HTTP id 1246625; Thu, 01 Oct 2009 09:15:57 -0600
6, 37 -- From: "Tamara A Howard" {thoward-at-unm.edu}
6, 37 -- Subject: Re: [Microscopy] RE: TEM: Nickel Grids in 200kV TEM for
6, 37 -- immuno-gold
6, 37 -- To: TindallR-at-missouri.edu, "microscopy server"
6, 37 -- {Microscopy-at-microscopy.com}
6, 37 -- X-Mailer: CommuniGate Pro WebUser v5.2.15
6, 37 -- Date: Thu, 01 Oct 2009 09:15:57 -0600
6, 37 -- Message-ID: {web-1246638-at-ceaster.unm.edu}
6, 37 -- In-Reply-To: {200910011458.n91EwNMt020057-at-ns.microscopy.com}
6, 37 -- References: {200910011458.n91EwNMt020057-at-ns.microscopy.com}
6, 37 -- MIME-Version: 1.0
6, 37 -- Content-Type: text/plain;charset=iso-8859-1;format="flowed"
6, 37 -- X-PMX-Version: x1 5.5.7.378829, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.1.150043
6, 37 -- X-PerlMx-Spam: Gauge=IIIIIIII, Probability=8%, Report='
6, 37 -- BODY_SIZE_10000_PLUS 0, FROM_EDU_TLD 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_ADDY_B 0, __FRAUD_419_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_GREETING 0, __PHISH_SPEAR_HTTP_RECEIVED 0, __PHISH_SPEAR_STRUCTURE_1 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0, __URI_NS '
6, 37 -- X-Junk-Score: [IIIIIIII]
6, 37 -- Content-Transfer-Encoding: 8bit
6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n91FFxKi001720
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Thu, 1 Oct 2009 10:31:08 -0500
Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have seen Ni grids escape from the spring loaded holder. It was a
Phillips 300 and we lost several of them that way. Since we were a rubber
company our cure was to move back to Cu grids.

stay safe.......
Frank




TindallR-at-missouri
.edu
To
10/01/2009 11:06 frank_karl-at-lincolnelectric.com
AM cc

Subject
Please respond to [Microscopy] RE: TEM: Nickel Grids
TindallR-at-missouri in 200kV TEM for immuno-gold
.edu












----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


I have never heard of any problems with grids being pulled out of the
holder by the field in the TEM. The likeliest problems are grids clinging
to forceps and occasional weirdness with image (distortions, etc.) with
severely magnetized grids.

If you just don't want to use nickel, gold grids will work well for immuno
work, but they're more expensive. Just don't try to label with ultra-small
gold probes and then use gold-enhancement to increase their visibility
after labeling. Obviously, the grid will be "enhanced" right along with
the gold nanos.

Finally, I have seen people use plain, old copper grids for labeling with
success. The idea that copper grids are not to be used for immunolabeling
is embedded in the EM psyche, but I have wondered many times how important
that really is. Never hurts to try alternatives, just for fun if nothing
else.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan

Sons of Norway: http://www.sofn.com.



-----Original Message-----
X-from: david.mitchell-at-emu.usyd.edu.au
[mailto:david.mitchell-at-emu.usyd.edu.au]
Sent: Wednesday, September 30, 2009 10:07 PM
To: Tindall, Randy D.


Dear All

We have some users who wish to examine biological sections mounted on
nickel
grids in our JEOL 2100 cryo TEM (200kV). These will be examined with either
a JEOL standard quick exchange holder or a JEOL non-analytical double tilt
holder. In both cases, the specimens are held in place by a retainer. This
is screwed down on one side only of the specimen. Retaining force is then
applied to the specimen via the residual springiness of the retainer.

Question 1: Is there any realistic possibility that a nickel grid may get
pulled out of the holder by the field and get stuck on the polepiece with
this type arrangement?

Question 2: The biological folk are using nickel grids for immunogold work.
I'm not a biologist, but it seems that using a ferromagnetic material for a
support grid, is best avoided wherever possible. Are there other types of
grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
immuno-gold labeling?

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com





==============================Original
Headers==============================
14, 27 -- From david.mitchell-at-emu.usyd.edu.au Wed Sep 30 22:05:12 2009
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au
[129.78.64.146])
14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n9135Bdc024824
14, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009
22:05:12 -0500
14, 27 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1])
14, 27 -- by localhost (Postfix) with SMTP id 09349103899
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009
13:05:09 +1000 (EST)
14, 27 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au
(ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
14, 27 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id
EA54F1039B1
14, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009
13:05:08 +1000 (EST)
14, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by
ex-bri-pro-01.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
14, 27 -- Thu, 1 Oct 2009 13:05:08 +1000
14, 27 -- Received: from 172.17.185.135 ([172.17.185.135]) by
EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server
www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server
HTTP-DAV ;
14, 27 -- Thu, 1 Oct 2009 03:05:08 +0000
14, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
14, 27 -- Date: Thu, 01 Oct 2009 13:05:07 +1000
14, 27 -- Subject: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
14, 27 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
14, 27 -- To: {Microscopy-at-microscopy.com}
14, 27 -- Message-ID: {C6EA5983.1983%david.mitchell-at-emu.usyd.edu.au}
14, 27 -- Thread-Topic: TEM: Nickel Grids in 200kV TEM for immuno-gold
labeling
14, 27 -- Thread-Index: AcpCQ/q2OUQPRK43Ed6dogAjMrpsqg==
14, 27 -- Mime-version: 1.0
14, 27 -- Content-type: text/plain;
14, 27 -- charset="US-ASCII"
14, 27 -- Content-transfer-encoding: 7bit
14, 27 -- X-OriginalArrivalTime: 01 Oct 2009 03:05:08.0655 (UTC) FILETIME=
[FBB33BF0:01CA4243]
==============================End of -
Headers==============================


==============================Original
Headers==============================
27, 35 -- From TindallR-at-missouri.edu Thu Oct 1 09:56:50 2009
27, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu
(mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
27, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n91Euo3K018155
27, 35 -- for {microscopy-at-microscopy.com} ; Thu, 1 Oct 2009
09:56:50 -0500
27, 35 -- X-IronPort-Anti-Spam-Filtered: true
27, 35 -- X-IronPort-Anti-Spam-Result:
ApoEAJNgxErRauUp/2dsb2JhbADILgEJhk6IS4JLgV4EgU+IOw
27, 35 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu)
([209.106.229.41])
27, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 01 Oct 2009
09:55:52 -0500
27, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
27, 35 -- Thu, 1 Oct 2009 09:55:12 -0500
27, 35 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
27, 35 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 1
Oct 2009
27, 35 -- 09:55:11 -0500
27, 35 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
27, 35 -- To: "'david.mitchell-at-emu.usyd.edu.au'"
{david.mitchell-at-emu.usyd.edu.au}
27, 35 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
27, 35 -- Date: Thu, 1 Oct 2009 09:55:10 -0500
27, 35 -- Subject: RE: [Microscopy] TEM: Nickel Grids in 200kV TEM for
immuno-gold
27, 35 -- labeling
27, 35 -- Thread-Topic: [Microscopy] TEM: Nickel Grids in 200kV TEM for
immuno-gold
27, 35 -- labeling
27, 35 -- Thread-Index: AcpCRC4NJdK6XQS8SBiUTFABEvWsnAAYasow
27, 35 -- Message-ID:
{9422E68616A7C648A281C0B5CD22A4B8129877C93A-at-UM-EMAIL06.um.umsystem.edu}
27, 35 -- References: {200910010306.n9136VXm026414-at-ns.microscopy.com}
27, 35 -- In-Reply-To: {200910010306.n9136VXm026414-at-ns.microscopy.com}
27, 35 -- Accept-Language: en-US
27, 35 -- Content-Language: en-US
27, 35 -- X-MS-Has-Attach:
27, 35 -- X-MS-TNEF-Correlator:
27, 35 -- acceptlanguage: en-US
27, 35 -- Content-Type: text/plain; charset="us-ascii"
27, 35 -- MIME-Version: 1.0
27, 35 -- X-OriginalArrivalTime: 01 Oct 2009 14:55:12.0108 (UTC) FILETIME=
[2D5642C0:01CA42A7]
27, 35 -- Content-Transfer-Encoding: 8bit
27, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n91Euo3K018155
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
50, 22 -- From frank_karl-at-lincolnelectric.com Thu Oct 1 10:31:08 2009
50, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
50, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91FV6aj016496
50, 22 -- for {microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 10:31:07 -0500
50, 22 -- In-Reply-To: {200910011506.n91F62YB031111-at-ns.microscopy.com}
50, 22 -- Subject: Re: [Microscopy] RE: TEM: Nickel Grids in 200kV TEM for immuno-gold
50, 22 -- To: TindallR-at-missouri.edu, Microscopy-at-microscopy.com
50, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
50, 22 -- Message-ID: {OF386EDB5E.C0FB7C70-ON85257642.0054EBB2-85257642.00553962-at-lincolnelectric.com}
50, 22 -- Date: Thu, 1 Oct 2009 11:31:21 -0400
50, 22 -- From: Frank_Karl-at-lincolnelectric.com
50, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
50, 22 -- 07, 2008) at 10/01/2009 11:30:52 AM,
50, 22 -- CD-MIME complete at 10/01/2009 11:30:52 AM,
50, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
50, 22 -- 07, 2008) at 10/01/2009 11:30:52 AM,
50, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
50, 22 -- 07, 2008) at 10/01/2009 11:30:52 AM,
50, 22 -- Serialize complete at 10/01/2009 11:30:52 AM
50, 22 -- MIME-Version: 1.0
50, 22 -- Content-Type: text/plain;
50, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: jsb43-at-cam.ac.uk
Date: Thu, 1 Oct 2009 10:52:14 -0500
Subject: [Microscopy] Vibration survey data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

At present, we are in consultation with architects about the design of
electron microscope laboratories. One of the issues that has yet to be
settled is the use of isolation joints or isolated concrete slabs on to
which high resolution TEMs are mounted, i.e. concrete slabs that are either
room sized or smaller: 3m by 4m for example.

The conventional wisdom has been "yes" you should use them, but we had a
set of site surveys performed and the data has been a real eye-opener:
Having isolated slabs makes the vibrations worse, especially at low
frequencies where the microscope specification become more stringent (see
NIST-A).

For example, we have found that a single isolated slab amplfies vibrations
in the frequency range [10Hz,70Hz] by a factor of between 2 to 5 times.

A doubly-isolated slab (slab within a slab) is even worse. The
amplification factor increases to 10 times for [5Hz,25Hz], especially in
the horizontal directions.

We were very surprised to see that when air-sprung systems are used, the
broad spectrum attenuation is accompanied by some nice amplification bands
that increase the vibration at certain eigenfrequencies (especially in the
few Hz range).

The moral of these surveys is that we should not use isolated slabs but opt
for one big massive slab on to which all the TEMs should be mounted.
However, the isolated slab concept seems to be widespread.

My question to you is this. Do you have vibration data for isloated slabs,
air sprung systems or any other vibration 'isolation' system that you are
willing to share? I'm looking for data not anecdotes.

For those of you interested in the data we have, we would be willing to
share it (with some discretion). Please contact me off-line for this.

Does the MSA have a set of guidelines/repository for EM building
specifications?


==============================Original Headers==============================
11, 25 -- From jsb43-at-hermes.cam.ac.uk Thu Oct 1 10:52:13 2009
11, 25 -- Received: from ppsw-6.csi.cam.ac.uk (ppsw-6.csi.cam.ac.uk [131.111.8.136])
11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91FqCS6031640
11, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 10:52:12 -0500
11, 25 -- X-Cam-AntiVirus: no malware found
11, 25 -- X-Cam-SpamDetails: not scanned
11, 25 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
11, 25 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:35242)
11, 25 -- by ppsw-6.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25)
11, 25 -- with esmtpa (EXTERNAL:jsb43) id 1MtNwy-0004tW-K8 (Exim 4.70) for Microscopy-at-microscopy.com
11, 25 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 01 Oct 2009 16:52:12 +0100
11, 25 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk)
11, 25 -- with local (PRAYER:jsb43) id 1MtNwy-0008Ff-7C (Exim 4.67) for Microscopy-at-microscopy.com
11, 25 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 01 Oct 2009 16:52:12 +0100
11, 25 -- Received: from [131.111.102.18] by webmail.hermes.cam.ac.uk
11, 25 -- with HTTP (Prayer-1.3.2); 01 Oct 2009 16:52:12 +0100
11, 25 -- Date: 01 Oct 2009 16:52:12 +0100
11, 25 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk}
11, 25 -- To: MSA Listserver {Microscopy-at-microscopy.com}
11, 25 -- Subject: Vibration survey data
11, 25 -- Message-ID: {Prayer.1.3.2.0910011652120.14346-at-hermes-1.csi.cam.ac.uk}
11, 25 -- X-Mailer: Prayer v1.3.2
11, 25 -- Mime-Version: 1.0
11, 25 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1
11, 25 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk}
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Thu, 1 Oct 2009 12:53:01 -0500
Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

From my experience, copper grids are perfectly fine as long as they
have support film covering the copper and your incubations are not too
long. If PBS is allowed extended contact with copper (like overnight),
yes, I saw the drops turn bluish - and then the grids very dirty under
the microscope. But if you invest a little time in making your formvar
film solid, should be no problem, and definitely worth the handling
convenience.
"Copper dirt" is something you either have or don't have, and you'll
know if you have it, so I would not worry about that simply as a
possibility.

A good alternative to Ni that I have found is gold-gilded copper
grids. I remember, a few years ago, someone on this list was warning
me how perilous could this be should the Au coat get scratched enough
for the Cu underneath to get exposed - all kinds of electrochemical
forces will be unleashed - but this has never happened to me. Gilded
copper grids are easy to handle and, unlike Ni, they take formvar
coating well.

Finally, the only case when you will really need Ni grids is if you
use Ultrasmall or Nanogold label and grow it by silver or gold
enhancement. But if you were doing that, you would not be asking the
question?.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

Dear All

We have some users who wish to examine biological sections mounted on
nickel
grids in our JEOL 2100 cryo TEM (200kV). These will be examined with
either
a JEOL standard quick exchange holder or a JEOL non-analytical double
tilt
holder. In both cases, the specimens are held in place by a retainer.
This
is screwed down on one side only of the specimen. Retaining force is
then
applied to the specimen via the residual springiness of the retainer.

Question 1: Is there any realistic possibility that a nickel grid may
get
pulled out of the holder by the field and get stuck on the polepiece
with
this type arrangement?

Question 2: The biological folk are using nickel grids for immunogold
work.
I'm not a biologist, but it seems that using a ferromagnetic material
for a
support grid, is best avoided wherever possible. Are there other types
of
grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
immuno-gold labeling?

Thanks and regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com

==============================Original Headers==============================
17, 22 -- From vladislav_speransky-at-nih.gov Thu Oct 1 12:53:01 2009
17, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25])
17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91Hr1R8018340
17, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 12:53:01 -0500
17, 22 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
17, 22 -- by gateway1.messagingengine.com (Postfix) with ESMTP id E96BC8206C;
17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
17, 22 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
17, 22 -- by compute2.internal (MEProxy); Thu, 01 Oct 2009 13:53:00 -0400
17, 22 -- X-Sasl-enc: qILOpX615WS8677ZNYlUvVpVnqY1//BJP+xAXqf4kSyn 1254419580
17, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
17, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 91338427C7;
17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
17, 22 -- Message-Id: {14A9CD70-79CA-41B7-B858-1BE17284564C-at-nih.gov}
17, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
17, 22 -- To: Microscopy-at-microscopy.com
17, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
17, 22 -- Content-Transfer-Encoding: 7bit
17, 22 -- Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
17, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
17, 22 -- Date: Thu, 1 Oct 2009 13:53:00 -0400
17, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: jteshima-at-dunesciences.com
Date: Thu, 1 Oct 2009 13:27:54 -0500
Subject: [Microscopy] Fwd: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

First, I should say that I am on the vendor side of things. Dune
Sciences makes chemically functionalized Silicon grids with silicon
dioxide membranes. There are other suppliers.
We have customers using our grids for immunoEM. Have any of you
considered silicon grids, they seem to be inert with respect to
reagents tested and the fims more robust than formvar after } 20 steps?
Janet


Janet Teshima
SMART Grids Product ManagerimmuoEM
cell: 503-5447526
office: 541-636-3712
jteshima-at-dunesciences.com
www.dunesciences.com
Begin forwarded message:

} From: vladislav_speransky-at-nih.gov
} Date: October 1, 2009 11:01:02 AM PDT
} To: jteshima-at-dunesciences.com
} Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-
} gold labeling
} Reply-To: vladislav_speransky-at-nih.gov
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} From my experience, copper grids are perfectly fine as long as they
} have support film covering the copper and your incubations are not too
} long. If PBS is allowed extended contact with copper (like overnight),
} yes, I saw the drops turn bluish - and then the grids very dirty under
} the microscope. But if you invest a little time in making your formvar
} film solid, should be no problem, and definitely worth the handling
} convenience.
} "Copper dirt" is something you either have or don't have, and you'll
} know if you have it, so I would not worry about that simply as a
} possibility.
}
} A good alternative to Ni that I have found is gold-gilded copper
} grids. I remember, a few years ago, someone on this list was warning
} me how perilous could this be should the Au coat get scratched enough
} for the Cu underneath to get exposed - all kinds of electrochemical
} forces will be unleashed - but this has never happened to me. Gilded
} copper grids are easy to handle and, unlike Ni, they take formvar
} coating well.
}
} Finally, the only case when you will really need Ni grids is if you
} use Ultrasmall or Nanogold label and grow it by silver or gold
} enhancement. But if you were doing that, you would not be asking the
} question?.
}
} Vlad
} ________________________________________________
} Vlad Speransky, Staff Scientist
} Supramolecular Structure and Function Resource
} National Institute of Biomedical Imaging and Bioengineering, NIH
} 13 South Dr, Rm. 3N17 MSC 5766
} Bethesda, MD 20892
} 301 496-3989
} vladislav_speransky-at-nih.gov
}
} Opinions and experiences related are those of Vlad Speransky and do
} not represent the NIH. On the good side, this message is not
} confidential and can be freely shared and reproduced.
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All
}
} We have some users who wish to examine biological sections mounted on
} nickel
} grids in our JEOL 2100 cryo TEM (200kV). These will be examined with
} either
} a JEOL standard quick exchange holder or a JEOL non-analytical double
} tilt
} holder. In both cases, the specimens are held in place by a retainer.
} This
} is screwed down on one side only of the specimen. Retaining force is
} then
} applied to the specimen via the residual springiness of the retainer.
}
} Question 1: Is there any realistic possibility that a nickel grid may
} get
} pulled out of the holder by the field and get stuck on the polepiece
} with
} this type arrangement?
}
} Question 2: The biological folk are using nickel grids for immunogold
} work.
} I'm not a biologist, but it seems that using a ferromagnetic material
} for a
} support grid, is best avoided wherever possible. Are there other types
} of
} grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
} immuno-gold labeling?
}
} Thanks and regards,
}
} Dave Mitchell
}
}
} Dr David Mitchell
} Senior Microscopist, Transmission Electron Microscopy
}
} Contact:
} PH +61 2 9036 7633
} FAX +61 2 9351 7682
} David.mitchell-at-emu.usyd.edu.au
}
} Address:
} Electron Microscope Unit
} Australian Key Centre for Microscopy and Microanalysis
} Australian Microscopy & Microanalysis Research Facility (AMMRF)
} Madsen Building F09, Room 111A
} The University of Sydney
} NSW 2006, Australia
} www.emu.usyd.edu.au
} www.ammrf.org.au
}
} www.dmscripting.com
}
} ==============================Original
} Headers==============================
} 17, 22 -- From vladislav_speransky-at-nih.gov Thu Oct 1 12:53:01 2009
} 17, 22 -- Received: from out1.smtp.messagingengine.com
} (out1.smtp.messagingengine.com [66.111.4.25])
} 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n91Hr1R8018340
} 17, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 12:53:01
} -0500
} 17, 22 -- Received: from compute2.internal (compute2.internal
} [10.202.2.42])
} 17, 22 -- by gateway1.messagingengine.com (Postfix) with ESMTP id
} E96BC8206C;
} 17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
} 17, 22 -- Received: from heartbeat1.messagingengine.com
} ([10.202.2.160])
} 17, 22 -- by compute2.internal (MEProxy); Thu, 01 Oct 2009
} 13:53:00 -0400
} 17, 22 -- X-Sasl-enc: qILOpX615WS8677ZNYlUvVpVnqY1//BJP+xAXqf4kSyn
} 1254419580
} 17, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov
} [128.231.217.59])
} 17, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id
} 91338427C7;
} 17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
} 17, 22 -- Message-Id: {14A9CD70-79CA-41B7-B858-1BE17284564C-at-nih.gov}
} 17, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
} 17, 22 -- To: Microscopy-at-microscopy.com
} 17, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes
} 17, 22 -- Content-Transfer-Encoding: 7bit
} 17, 22 -- Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for
} immuno-gold labeling
} 17, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
} 17, 22 -- Date: Thu, 1 Oct 2009 13:53:00 -0400
} 17, 22 -- X-Mailer: Apple Mail (2.936)
} ==============================End of -
} Headers==============================






==============================Original Headers==============================
9, 22 -- From jteshima-at-dunesciences.com Thu Oct 1 13:27:54 2009
9, 22 -- Received: from mail-px0-f187.google.com (mail-px0-f187.google.com [209.85.216.187])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91IRs16001398
9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:27:54 -0500
9, 22 -- Received: by pxi17 with SMTP id 17so486139pxi.21
9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 01 Oct 2009 11:27:53 -0700 (PDT)
9, 22 -- Received: by 10.114.18.33 with SMTP id 33mr2607625war.51.1254421673386;
9, 22 -- Thu, 01 Oct 2009 11:27:53 -0700 (PDT)
9, 22 -- Received: from ?192.168.1.101? (c-67-160-157-47.hsd1.or.comcast.net [67.160.157.47])
9, 22 -- by mx.google.com with ESMTPS id 22sm181500pxi.6.2009.10.01.11.27.52
9, 22 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
9, 22 -- Thu, 01 Oct 2009 11:27:52 -0700 (PDT)
9, 22 -- Message-Id: {93D75F22-B114-4B01-95DA-14DCBFF83625-at-dunesciences.com}
9, 22 -- From: Janet Teshima {jteshima-at-dunesciences.com}
9, 22 -- To: Microscopy-at-microscopy.com
9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
9, 22 -- Subject: Fwd: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
9, 22 -- Date: Thu, 1 Oct 2009 11:27:51 -0700
9, 22 -- References: {200910011801.n91I1279031937-at-ns.microscopy.com}
9, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Thu, 1 Oct 2009 13:39:22 -0500
Subject: [Microscopy] Fwd: TEM: Nickel Grids in 200kV TEM for immuno-gold labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Silicon sounds cool but at $30/grid, one would be somewhat restricted in the number of samples and conditions that would be practical!

As others have said, I have tried (my mistake) copper grids for EM immune and had precipitation problems that I don't think were linked to Tris based buffers. I use mostly HEPES or PBS and not sure which type caused the problem but I use nickel exclusively for immune.

Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/



-----Original Message-----
X-from: jteshima-at-dunesciences.com [mailto:jteshima-at-dunesciences.com]
Sent: Thursday, October 01, 2009 1:29 PM
To: Phillips, Thomas E.

First, I should say that I am on the vendor side of things. Dune
Sciences makes chemically functionalized Silicon grids with silicon
dioxide membranes. There are other suppliers.
We have customers using our grids for immunoEM. Have any of you
considered silicon grids, they seem to be inert with respect to
reagents tested and the fims more robust than formvar after } 20 steps?
Janet


Janet Teshima
SMART Grids Product ManagerimmuoEM
cell: 503-5447526
office: 541-636-3712
jteshima-at-dunesciences.com
www.dunesciences.com
Begin forwarded message:

} From: vladislav_speransky-at-nih.gov
} Date: October 1, 2009 11:01:02 AM PDT
} To: jteshima-at-dunesciences.com
} Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-
} gold labeling
} Reply-To: vladislav_speransky-at-nih.gov
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} From my experience, copper grids are perfectly fine as long as they
} have support film covering the copper and your incubations are not too
} long. If PBS is allowed extended contact with copper (like overnight),
} yes, I saw the drops turn bluish - and then the grids very dirty under
} the microscope. But if you invest a little time in making your formvar
} film solid, should be no problem, and definitely worth the handling
} convenience.
} "Copper dirt" is something you either have or don't have, and you'll
} know if you have it, so I would not worry about that simply as a
} possibility.
}
} A good alternative to Ni that I have found is gold-gilded copper
} grids. I remember, a few years ago, someone on this list was warning
} me how perilous could this be should the Au coat get scratched enough
} for the Cu underneath to get exposed - all kinds of electrochemical
} forces will be unleashed - but this has never happened to me. Gilded
} copper grids are easy to handle and, unlike Ni, they take formvar
} coating well.
}
} Finally, the only case when you will really need Ni grids is if you
} use Ultrasmall or Nanogold label and grow it by silver or gold
} enhancement. But if you were doing that, you would not be asking the
} question?.
}
} Vlad
} ________________________________________________
} Vlad Speransky, Staff Scientist
} Supramolecular Structure and Function Resource
} National Institute of Biomedical Imaging and Bioengineering, NIH
} 13 South Dr, Rm. 3N17 MSC 5766
} Bethesda, MD 20892
} 301 496-3989
} vladislav_speransky-at-nih.gov
}
} Opinions and experiences related are those of Vlad Speransky and do
} not represent the NIH. On the good side, this message is not
} confidential and can be freely shared and reproduced.
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All
}
} We have some users who wish to examine biological sections mounted on
} nickel
} grids in our JEOL 2100 cryo TEM (200kV). These will be examined with
} either
} a JEOL standard quick exchange holder or a JEOL non-analytical double
} tilt
} holder. In both cases, the specimens are held in place by a retainer.
} This
} is screwed down on one side only of the specimen. Retaining force is
} then
} applied to the specimen via the residual springiness of the retainer.
}
} Question 1: Is there any realistic possibility that a nickel grid may
} get
} pulled out of the holder by the field and get stuck on the polepiece
} with
} this type arrangement?
}
} Question 2: The biological folk are using nickel grids for immunogold
} work.
} I'm not a biologist, but it seems that using a ferromagnetic material
} for a
} support grid, is best avoided wherever possible. Are there other types
} of
} grid (non-ferromagnetic - Al, Mo, nylon etc) which are compatible with
} immuno-gold labeling?
}
} Thanks and regards,
}
} Dave Mitchell
}
}
} Dr David Mitchell
} Senior Microscopist, Transmission Electron Microscopy
}
} Contact:
} PH +61 2 9036 7633
} FAX +61 2 9351 7682
} David.mitchell-at-emu.usyd.edu.au
}
} Address:
} Electron Microscope Unit
} Australian Key Centre for Microscopy and Microanalysis
} Australian Microscopy & Microanalysis Research Facility (AMMRF)
} Madsen Building F09, Room 111A
} The University of Sydney
} NSW 2006, Australia
} www.emu.usyd.edu.au
} www.ammrf.org.au
}
} www.dmscripting.com
}
} ==============================Original
} Headers==============================
} 17, 22 -- From vladislav_speransky-at-nih.gov Thu Oct 1 12:53:01 2009
} 17, 22 -- Received: from out1.smtp.messagingengine.com
} (out1.smtp.messagingengine.com [66.111.4.25])
} 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n91Hr1R8018340
} 17, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 12:53:01
} -0500
} 17, 22 -- Received: from compute2.internal (compute2.internal
} [10.202.2.42])
} 17, 22 -- by gateway1.messagingengine.com (Postfix) with ESMTP id
} E96BC8206C;
} 17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
} 17, 22 -- Received: from heartbeat1.messagingengine.com
} ([10.202.2.160])
} 17, 22 -- by compute2.internal (MEProxy); Thu, 01 Oct 2009
} 13:53:00 -0400
} 17, 22 -- X-Sasl-enc: qILOpX615WS8677ZNYlUvVpVnqY1//BJP+xAXqf4kSyn
} 1254419580
} 17, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov
} [128.231.217.59])
} 17, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id
} 91338427C7;
} 17, 22 -- Thu, 1 Oct 2009 13:53:00 -0400 (EDT)
} 17, 22 -- Message-Id: {14A9CD70-79CA-41B7-B858-1BE17284564C-at-nih.gov}
} 17, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
} 17, 22 -- To: Microscopy-at-microscopy.com
} 17, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
} delsp=yes
} 17, 22 -- Content-Transfer-Encoding: 7bit
} 17, 22 -- Subject: [Microscopy] TEM: Nickel Grids in 200kV TEM for
} immuno-gold labeling
} 17, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
} 17, 22 -- Date: Thu, 1 Oct 2009 13:53:00 -0400
} 17, 22 -- X-Mailer: Apple Mail (2.936)
} ==============================End of -
} Headers==============================






==============================Original Headers==============================
9, 22 -- From jteshima-at-dunesciences.com Thu Oct 1 13:27:54 2009
9, 22 -- Received: from mail-px0-f187.google.com (mail-px0-f187.google.com [209.85.216.187])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91IRs16001398
9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:27:54 -0500
9, 22 -- Received: by pxi17 with SMTP id 17so486139pxi.21
9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 01 Oct 2009 11:27:53 -0700 (PDT)
9, 22 -- Received: by 10.114.18.33 with SMTP id 33mr2607625war.51.1254421673386;
9, 22 -- Thu, 01 Oct 2009 11:27:53 -0700 (PDT)
9, 22 -- Received: from ?192.168.1.101? (c-67-160-157-47.hsd1.or.comcast.net [67.160.157.47])
9, 22 -- by mx.google.com with ESMTPS id 22sm181500pxi.6.2009.10.01.11.27.52
9, 22 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
9, 22 -- Thu, 01 Oct 2009 11:27:52 -0700 (PDT)
9, 22 -- Message-Id: {93D75F22-B114-4B01-95DA-14DCBFF83625-at-dunesciences.com}
9, 22 -- From: Janet Teshima {jteshima-at-dunesciences.com}
9, 22 -- To: Microscopy-at-microscopy.com
9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
9, 22 -- Subject: Fwd: [Microscopy] TEM: Nickel Grids in 200kV TEM for immuno-gold labeling
9, 22 -- Date: Thu, 1 Oct 2009 11:27:51 -0700
9, 22 -- References: {200910011801.n91I1279031937-at-ns.microscopy.com}
9, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================


==============================Original Headers==============================
24, 35 -- From PhillipsT-at-missouri.edu Thu Oct 1 13:39:22 2009
24, 35 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
24, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n91IdLv9015997
24, 35 -- for {microscopy-at-microscopy.com} ; Thu, 1 Oct 2009 13:39:21 -0500
24, 35 -- X-IronPort-Anti-Spam-Filtered: true
24, 35 -- X-IronPort-Anti-Spam-Result: ApoEAKuTxErRauUp/2dsb2JhbADIQQEMAQmGSYhLAoJJgV4EgU8
24, 35 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
24, 35 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 01 Oct 2009 13:39:21 -0500
24, 35 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
24, 35 -- Thu, 1 Oct 2009 13:39:21 -0500
24, 35 -- Received: from um-email05.um.umsystem.edu ([169.254.1.45]) by
24, 35 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Thu, 1 Oct 2009
24, 35 -- 13:39:20 -0500
24, 35 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
24, 35 -- To: "jteshima-at-dunesciences.com" {jteshima-at-dunesciences.com} ,
24, 35 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
24, 35 -- Date: Thu, 1 Oct 2009 13:39:20 -0500
24, 35 -- Subject: RE: [Microscopy] Fwd: TEM: Nickel Grids in 200kV TEM for
24, 35 -- immuno-gold labeling
24, 35 -- Thread-Topic: [Microscopy] Fwd: TEM: Nickel Grids in 200kV TEM for
24, 35 -- immuno-gold labeling
24, 35 -- Thread-Index: AcpCxP96Bzv834MPSPCrpyA9NOheqAAAQIbg
24, 35 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF07E039123F-at-UM-EMAIL05.um.umsystem.edu}
24, 35 -- References: {200910011828.n91ISapb002446-at-ns.microscopy.com}
24, 35 -- In-Reply-To: {200910011828.n91ISapb002446-at-ns.microscopy.com}
24, 35 -- Accept-Language: en-US
24, 35 -- Content-Language: en-US
24, 35 -- X-MS-Has-Attach:
24, 35 -- X-MS-TNEF-Correlator:
24, 35 -- acceptlanguage: en-US
24, 35 -- Content-Type: text/plain; charset="us-ascii"
24, 35 -- MIME-Version: 1.0
24, 35 -- X-OriginalArrivalTime: 01 Oct 2009 18:39:21.0094 (UTC) FILETIME=[7D8EAA60:01CA42C6]
24, 35 -- Content-Transfer-Encoding: 8bit
24, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n91IdLv9015997
==============================End of - Headers==============================




From: dhorne-at-interchange.ubc.ca
Date: Fri, 2 Oct 2009 17:04:08 -0500
Subject: [Microscopy] viaWWW: Creating Client Satisfaction Surveys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dhorne-at-interchange.ubc.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] Client Satisfaction Surveys

Question: In the ongoing effort to ensure we provide excellent
service, I would like to ask our clients to reflect on their
interaction with the staff and equipment in our facility.

Does anyone have experience creating and using client satisfaction
surveys? If so, would you be willing to share any details concerning
your survey?

Utility? Questions asked? Results? Biggest surprises?

Derrick Horne
Senior EM Technician
UBC BioImaging Facility



Login Host: 137.82.85.214
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Oct 2 17:04:08 2009
11, 11 -- Received: from [172.20.100.23] ([130.202.238.72])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n92M44JE008310
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 2 Oct 2009 17:04:06 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240806c6ec291f420f-at-[172.20.100.23]}
11, 11 -- Date: Sat, 3 Oct 2009 07:04:04 +0900
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Creating Client Satisfaction Surveys
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dwaugh-at-kent.edu
Date: Sat, 3 Oct 2009 15:07:28 -0500
Subject: [Microscopy] BSE Diodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a Amray 1645 with 4 BSE diodes that have broken leads (the
REALLY small gold wires, not the larger ones). These are way too small
to solder without special equipment. The preamp and amp are working
and I would like to move the system to our Amray 1600. Ideally we
would just buy a new detector, but as you all know, budgets are
tight... Does anyone know of some off the shelf diodes that could be
used for a homemade BSE detector. There are lots of small electronic
parts dealers, I'm assuming that some of the diodes they would work
and not be too expensive. Any ideas, Thanks, David

==============================Original Headers==============================
1, 17 -- From dwaugh-at-kent.edu Sat Oct 3 15:07:28 2009
1, 17 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n93K7Sah025131
1, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Oct 2009 15:07:28 -0500
1, 17 -- Received: from [192.168.1.102] (really [204.210.197.254])
1, 17 -- by cdptpa-omta02.mail.rr.com with ESMTP
1, 17 -- id {20091003200727803.IWVM6584-at-cdptpa-omta02.mail.rr.com}
1, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Oct 2009 20:07:27 +0000
1, 17 -- Message-Id: {B56FA324-F40B-4149-B309-7475BD3BDB2A-at-kent.edu}
1, 17 -- From: David Waugh {dwaugh-at-kent.edu}
1, 17 -- To: Microscopy-at-microscopy.com
1, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
1, 17 -- Content-Transfer-Encoding: 7bit
1, 17 -- Mime-Version: 1.0 (Apple Message framework v936)
1, 17 -- Subject: BSE Diodes
1, 17 -- Date: Sat, 3 Oct 2009 16:07:27 -0400
1, 17 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Mon, 5 Oct 2009 05:01:01 -0500
Subject: [Microscopy] Re: mouse embryo TEM protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Larry!

As a rule of thumb it is considered that glutaraldehyde penetrates the tissue at a rate of 1mm/hour. This is the reason why (1) Formaldehyde is used with glutar: it fixes less well but penetrates faster (2) it is advised to cut the tissue in small pieces. Or, in the case of organs (or organisms), to use perfusion. The penetration rate of osmiumtetroxide is believed to be approx. 2x slower!
Of course these values are means, it is evident that the penetration rate is different in different tissues. I would tend to believe that leaving the entire embryos in the fixation solution is no good idea, because the skin is a tight (keratinocytes are tightly bound with junctions, basal membrane...), water-repellent structure and I wouldn't expect the penetration rate to be extraordinarily fast through this tissue.
It would probably be OK if you just want to study the skin itself, but anything under it should be better fixed "from within", whether by dissecting the organs or by perfusing the embryo.
To better visualize membrane components, please consider adding ferrocyanate in your fixation solution.
I am sorry to be so theoretical, but shouldn't experimental design be firstly based on strong theoretical grounds?

Best regards,

Stephane

----- Original Message ----
X-from: "larry.ackerman-at-ucsf.edu" {larry.ackerman-at-ucsf.edu}
To: nizets2-at-yahoo.com
Sent: Wednesday, September 30, 2009 11:56:11 PM

I was not quite clear. The failed experiment tissue was in fix for 4 or
5 days before I received it. The lengthy fix has been okay with other
adult tissues but not this sample. The first clue was that the tissue
did not turn black in osmium just pale brown. The sections had extremely
low contrast with no membranes visible. This project is looking at
cellular junctional complexes.

Larry

Markus F. Meyenhofer wrote:
} 2 hours in Fixative is too long?? I don't (and many others) think so!
} What does "unusable" mean?
} Please explain.
} Regards,
} Markus F. Meyenhofer
} Microscopy Labs
} Red Bank, NJ
}
}
} ----- Original Message -----
} From: {larry.ackerman-at-ucsf.edu}
} To: {micro-at-superlink.net}
} Sent: Wednesday, September 30, 2009 5:19 PM
} Subject: [Microscopy] mouse embryo TEM protocol
}
}
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- 
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi,
} } I need some feedback for a protocol to process E12.5 mouse embryos for
} } TEM. The first batch was in fix too long and was unusable. The critical
} } factors I am asking about are fixative concentrations, times and buffer
} } molarity. No speculation please, only results based on actual successful
} } experiments.
} }
} } The provisional protocol is below:
} } Thanks,
} } Larry
} }
} } Protocol for mouse embryo electron microscopy October 1, 2009
} }
} } Primary fixation:
} }
} } 4% formaldehyde, freshly made or EM grade, methanol free
} } 3% EM grade glutaraldehyde
} } O.1M PO4 buffer, pH 7.2
} } Room temperature, immersion for no more than two hours, slice embryos in
} } half with sharp razor blade after 10 or 15 minutes in fix
} }
} } Rinse:
} } 01.M PO4, pH 7.2 3X 10 minutes
} }
} } 1% OsO4 in 0.1M PO4 2 hours-tissue should turn black
} }
} } Rinse:
} } 01.M PO4, pH 7.2 3X 5 minutes
} }
} } 1X DH2O 5 minutes
} }
} } enblock stain:
} } 2% uranyl acetate in H2O one hour
} }
} } Dehydrate:
} }
} } 35% ethanol 10 minutes
} } 70% ethanol 10 minutes
} } 95% ethanol 10 minutes
} } 100% ethanol 10 minutes X3
} }
} } propylene oxide 2X 10 minutes
} }
} } epoxy resin : propylene oxide 2X  1:1  (EMBed812 or similar resin) two
} } hours or overnight
} }
} } fresh resin two-four hours
} }
} } fresh resin in embedding mold
} }
} } polymerize 60 degrees C 48 hours
} }
} } ==============================Original
} } Headers==============================
} } 17, 30 -- From Larry.Ackerman-at-ucsf.edu Wed Sep 30 16:19:31 2009
} } 17, 30 -- Received: from bcuda4.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
} } 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } n8ULJUHC030296
} } 17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:19:31 -0500
} } 17, 30 -- X-ASG-Debug-ID: 1254345569-0aaa01c90000-1DjkGe
} } 17, 30 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
} } 17, 30 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
} } 17, 30 -- by bcuda4.ucsf.edu (Spam & Virus Firewall) with ESMTP id
} } 2471F10D5A84
} } 17, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700
} } (PDT)
} } 17, 30 -- Received: from EXHT03.net.ucsf.edu (mx.ucsf.edu [64.54.247.193])
} } by bcuda4.ucsf.edu with ESMTP id 0iszrBkl7IjZZnmv for
} } {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:19:29 -0700 (PDT)
} } 17, 30 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
} } 17, 30 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
} } 17, 30 -- Received: from Ralston-Lab-iMac.local (128.218.239.124) by
} } EXHT03.net.ucsf.edu
} } 17, 30 --  (64.54.247.220) with Microsoft SMTP Server id 8.1.393.1; Wed,
} } 30 Sep 2009
} } 17, 30 --  14:19:29 -0700
} } 17, 30 -- Message-ID: {4AC3CB56.7050705-at-ucsf.edu}
} } 17, 30 -- Date: Wed, 30 Sep 2009 14:19:18 -0700
} } 17, 30 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
} } 17, 30 -- Reply-To: larry.ackerman-at-ucsf.edu
} } 17, 30 -- Organization: UCSF, NeuroAnatomy
} } 17, 30 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
} } 17, 30 -- MIME-Version: 1.0
} } 17, 30 -- To: {Microscopy-at-microscopy.com}
} } 17, 30 -- X-ASG-Orig-Subj: mouse embryo TEM protocol
} } 17, 30 -- Subject: mouse embryo TEM protocol
} } 17, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
} } 17, 30 -- Content-Transfer-Encoding: 8bit
} } 17, 30 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
} } 17, 30 -- X-Barracuda-Start-Time: 1254345570
} } 17, 30 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at
} } ucsf.edu
} } ==============================End of -
} } Headers==============================
} }
}
} .
}

==============================Original Headers==============================
3, 32 -- From Larry.Ackerman-at-ucsf.edu Wed Sep 30 16:51:45 2009
3, 32 -- Received: from bcuda4.ucsf.edu (bcuda4.ucsf.edu [64.54.247.216])
3, 32 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n8ULpihJ014028
3, 32 --     for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 16:51:45 -0500
3, 32 -- X-ASG-Debug-ID: 1254347504-0aa5023e0000-1DjkGe
3, 32 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
3, 32 -- Received: from EXHT03.net.ucsf.edu (localhost [127.0.0.1])
3, 32 --     by bcuda4.ucsf.edu (Spam & Virus Firewall) with ESMTP id 59BD310D67A3
3, 32 --     for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:51:44 -0700 (PDT)
3, 32 -- Received: from EXHT03.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda4.ucsf.edu with ESMTP id RrXIKTU6ZQnCd1Pi for {Microscopy-at-microscopy.com} ; Wed, 30 Sep 2009 14:51:44 -0700 (PDT)
3, 32 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
3, 32 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
3, 32 -- Received: from Ralston-Lab-iMac.local (128.218.239.124) by EXHT03.net.ucsf.edu
3, 32 --  (64.54.247.220) with Microsoft SMTP Server id 8.1.393.1; Wed, 30 Sep 2009
3, 32 --  14:51:42 -0700
3, 32 -- Message-ID: {4AC3D2E2.8010507-at-ucsf.edu}
3, 32 -- Date: Wed, 30 Sep 2009 14:51:30 -0700
3, 32 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
3, 32 -- Reply-To: larry.ackerman-at-ucsf.edu
3, 32 -- Organization: UCSF, NeuroAnatomy
3, 32 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
3, 32 -- MIME-Version: 1.0
3, 32 -- To: {Microscopy-at-microscopy.com}
3, 32 -- X-ASG-Orig-Subj: Re: [Microscopy] mouse embryo TEM protocol
3, 32 -- Subject: Re: [Microscopy] mouse embryo TEM protocol
3, 32 -- References: {200909302119.n8ULJi9u030572-at-ns.microscopy.com} {B406ADFEE90C42BF8E7ADF0F8C166187-at-DJ4VDH31}
3, 32 -- In-Reply-To: {B406ADFEE90C42BF8E7ADF0F8C166187-at-DJ4VDH31}
3, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
3, 32 -- Content-Transfer-Encoding: 7bit
3, 32 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
3, 32 -- X-Barracuda-Start-Time: 1254347504
3, 32 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================






==============================Original Headers==============================
17, 25 -- From nizets2-at-yahoo.com Mon Oct 5 05:01:01 2009
17, 25 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239])
17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n95A100L008856
17, 25 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 05:01:00 -0500
17, 25 -- Received: (qmail 50113 invoked by uid 60001); 5 Oct 2009 10:01:00 -0000
17, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1254736860; bh=GjEuzYZhAI5AiZHq4UmoBj4RYqvc9c1AwaDIfLKHFLo=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jYJWrhQAsjrJ/4U76HgPsmSst5zPcYyqtfZdGG9nWQaWf0XTmfIQoShCwwBEOOQJ9x1qxnMxE5mfOjrGHzTeoHJfjtHXCJT+rWQNh9W2wGtlnCHOiXBUafBuqa/lo0Y7of6VKPQF27soZG7RlX+MaL+OshKuLVCggfcSXE/fO6I=
17, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
17, 25 -- s=s1024; d=yahoo.com;
17, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
17, 25 -- b=6rGWxZ1j7wl+iZpN5nVXgEb4Z3vLGWHcfNBOk2CtWHeE6ieE6Cb2Lis+mG12yJkwMgqMhZlrMrGgA6UnNCplRWhyo8bt9ZFPUqcjJOPZa1Dfln57bU4V4uqUX5+jeavEUyZ3vIMjrNJ9ygSRPXyuSa5Pe6bgfXqzZKCYJBiqKiY=;
17, 25 -- Message-ID: {141270.47705.qm-at-web110816.mail.gq1.yahoo.com}
17, 25 -- X-YMail-OSG: .v_5bbYVM1mmeTL4ujy9cT39bPSq_EqSouhUArsHe0dWG1X3NMBoMB6Z_Ec8bEfytTauHATxPpeywNS7WcI8hOn.IpEEXmbXJDMPNUJY9KVAW5t3lJ6e6ZtDVM8eW48C0HbNlh_bh7qPjN8PwvyXWqB53ClfE.rSFl4UxMdBHtgzhkny60B6CUCwzkTBDmzEh0ul5NTWRPEYd4psONpsfk.pzsH6nyIRoXzFDwDrVCQDCeKU_B5mG2P4tCcRWsQ5ucxMyBn28sH.3xpm29uCqRRpb4Pr3OnEOPcsfKJ_cLpXXPHqgYlzNW.6wBf7axRsWmJ0l2Y6x3DkKhAYEAm75pfxvOHH2kTQ1eHBpDTqzpinMzSfuCLQyy9KzA--
17, 25 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Mon, 05 Oct 2009 03:01:00 PDT
17, 25 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.347.3
17, 25 -- References: {200909302156.n8ULuBMa020610-at-ns.microscopy.com}
17, 25 -- Date: Mon, 5 Oct 2009 03:01:00 -0700 (PDT)
17, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
17, 25 -- Subject: Re: [Microscopy] Re: mouse embryo TEM protocol
17, 25 -- To: larry.ackerman-at-ucsf.edu
17, 25 -- Cc: microscopy-at-microscopy.com
17, 25 -- In-Reply-To: {200909302156.n8ULuBMa020610-at-ns.microscopy.com}
17, 25 -- MIME-Version: 1.0
17, 25 -- Content-Type: text/plain; charset=iso-8859-1
17, 25 -- Content-Transfer-Encoding: 8bit
17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n95A100L008856
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Mon, 5 Oct 2009 06:42:49 -0500
Subject: [Microscopy] Re: mouse embryo TEM protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello and good morning all,

Dear Larry,
I would like to know what the meaning of your sentence in your 2nd e-mail quest

"...The first clue was that the tissue did not turn black in osmium just pale brown. The sections had extremely low contrast with no membranes visible. " was/is.

Does it mean: ....did not turn (rapidly) black in osmium
(Initially / DURING / AFTER OsO4-incubation or
(ALSO) after / during Ethanol-Series?)
just pale brown...

In my experience (this means IF 1% OsO4 working = incubation solution has been made up freshly and and +/- chemically "clean") there always are tissues (parts of tissues, as found in human diagnostic samples) which are NOT "colored" black UNTIL they are at least in 50 or 70% ethanol. Sometimes I had tissue parts (e.g. skin biopsies) not stained black even in 96%Ethanol (for example: bare connective tissue, IF there were fat vacuoles/cells in that areas, those were black, but the surrounding connective tissue was not!).

You told us that you are fixing mouse embryos. Your protocol also is:
} } Primary fixation:
} }
} } 4% formaldehyde, freshly made or EM grade, methanol free
} } 3% EM grade glutaraldehyde
} } O.1M PO4 buffer, pH 7.2
} } Room temperature, immersion for no more than two hours, slice embryos in
} } half with sharp razor blade after 10 or 15 minutes in fix
} }
So, in my understanding your fixative = 2% FA-1.5 % GA in 0.05 M PO4-buffer or is ist end concentration really
4% FA-3%GA in 0.1 M PO4 buffer?

Have you checked the quality of GA-Fixative (source?, content of polymers?) since I found out the quality of GA to be of importance for a sufficient and high quality fixation process (also for getting good staining at the end of spec. preparation). Also the quality of OsO4-solution?

It might be (as Stephane said) that the skin barrier exerts really also a diffusion barrier for the fixative (at least for the first 10 to 15 mins, cf above) and ifyou divide the embryo in two halves I think the resulting pieces still are really "big" and "voluminous" compared to the penetration rates of immersed tissues.

Also one can assume that blackening of tissue as a consequence of OsO4-incubation in your fetal/embryonic tissue (you did tell us, 12.5 days gestational age the embryos are, assuming that I am right that delivery usual takes place 21-22 nd day of gestation) is not quite as rapid than with mature tissues due to the high water and connective tissue content.

} } This project is looking at cellular junctional complexes { {.
Do you look into skin-cellular junctional complexes or other junctions lying within the embryo?

It is perhaps also to take into consideration that another mixture of imersion-fixative (variation of concentrations, FA, GA as well as molarity of buffer) will result in better preservation of embryonal structures,
Assuming that a } fixation by perfusion { of embryos is NOT your choice.

Best of luck,
Wolfgang MUSS
EM-Lab SALK-LKH/PMU
SALZBURG, Austria





==============================Original Headers==============================
16, 35 -- From W.Muss-at-salk.at Mon Oct 5 06:42:47 2009
16, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95Bgjav028755
16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 06:42:46 -0500
16, 35 -- Received: from localhost (localhost [127.0.0.1])
16, 35 -- by hermes.salk.at (Postfix) with ESMTP id 2A7B1C3867;
16, 35 -- Mon, 5 Oct 2009 13:42:44 +0200 (CEST)
16, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
16, 35 -- Received: from hermes.salk.at ([127.0.0.1])
16, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
16, 35 -- with ESMTP id xigRKRGqPHgC; Mon, 5 Oct 2009 13:42:43 +0200 (CEST)
16, 35 -- Received: from n2rz123.lksdom21.lks.local (n2rz123.lksdom21.lks.local [192.168.101.123])
16, 35 -- by hermes.salk.at (Postfix) with ESMTP id A9AF2C3865;
16, 35 -- Mon, 5 Oct 2009 13:42:43 +0200 (CEST)
16, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.132]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
16, 35 -- Mon, 5 Oct 2009 13:42:43 +0200
16, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
16, 35 -- Content-class: urn:content-classes:message
16, 35 -- MIME-Version: 1.0
16, 35 -- Content-Type: text/plain;
16, 35 -- charset="iso-8859-1"
16, 35 -- Subject: Re: mouse embryo TEM protocol
16, 35 -- Date: Mon, 5 Oct 2009 13:42:43 +0200
16, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062938996-at-N1RZ116.lksdom21.lks.local}
16, 35 -- X-MS-Has-Attach:
16, 35 -- X-MS-TNEF-Correlator:
16, 35 -- Thread-Topic: Re: mouse embryo TEM protocol
16, 35 -- Thread-Index: AcpFsPNQoCo9H6b7QJ2b+XVYDGQVaw==
16, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
16, 35 -- To: {microscopy-at-microscopy.com}
16, 35 -- Cc: {larry.ackerman-at-ucsf.edu}
16, 35 -- X-OriginalArrivalTime: 05 Oct 2009 11:42:43.0741 (UTC) FILETIME=[F3A04CD0:01CA45B0]
16, 35 -- X-Scanned-By: SALK-Content-Filter
16, 35 -- Content-Transfer-Encoding: 8bit
16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n95Bgjav028755
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Mon, 5 Oct 2009 10:53:15 -0500
Subject: [Microscopy] Magnification Calibration of Digital Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I finally have a good problem. After 25 years of complaining, etc. to
the powers that be we finally are having a digital camera installed.
Based on the last 40 years of work I could probably design a protocol
for magnifiction calibration based on the film magnification protocols
quite readliy, but could then have some problems in application. I
would be very happy if anyone would be willing to share their protocols
so that I can do this right, and most efficiently the first time, rather
than taking until the 10th or 12th

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
5, 18 -- From paul_hazelton-at-umanitoba.ca Mon Oct 5 10:53:15 2009
5, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95Fr9fY023936
5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 10:53:14 -0500
5, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
5, 18 -- (authenticated bits=0)
5, 18 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n95FqpYh010698;
5, 18 -- Mon, 5 Oct 2009 10:52:55 -0500 (CDT)
5, 18 -- Message-ID: {4ACA1650.1070401-at-umanitoba.ca}
5, 18 -- Date: Mon, 05 Oct 2009 10:52:48 -0500
5, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 18 -- Subject: Magnification Calibration of Digital Systems
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
5, 18 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: PhillipsT-at-missouri.edu
Date: Mon, 5 Oct 2009 11:17:36 -0500
Subject: [Microscopy] Magnification Calibration of Digital Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You just need to photograph a slide micrometer with each objective. Open the images in Photoshop or its equivalent and determine the number of pixels that a known distance on the micrometer covers using the measure tool. You now can calculate the microns/pixel and post this next to your microscope. The original unmodified images will always be this size.


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Monday, October 05, 2009 10:54 AM
To: Phillips, Thomas E.

I finally have a good problem. After 25 years of complaining, etc. to
the powers that be we finally are having a digital camera installed.
Based on the last 40 years of work I could probably design a protocol
for magnifiction calibration based on the film magnification protocols
quite readliy, but could then have some problems in application. I
would be very happy if anyone would be willing to share their protocols
so that I can do this right, and most efficiently the first time, rather
than taking until the 10th or 12th

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
5, 18 -- From paul_hazelton-at-umanitoba.ca Mon Oct 5 10:53:15 2009
5, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95Fr9fY023936
5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 10:53:14 -0500
5, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
5, 18 -- (authenticated bits=0)
5, 18 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n95FqpYh010698;
5, 18 -- Mon, 5 Oct 2009 10:52:55 -0500 (CDT)
5, 18 -- Message-ID: {4ACA1650.1070401-at-umanitoba.ca}
5, 18 -- Date: Mon, 05 Oct 2009 10:52:48 -0500
5, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 18 -- Subject: Magnification Calibration of Digital Systems
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
5, 18 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================


==============================Original Headers==============================
16, 33 -- From PhillipsT-at-missouri.edu Mon Oct 5 11:17:35 2009
16, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
16, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95GHZeP006735
16, 33 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:17:35 -0500
16, 33 -- X-IronPort-Anti-Spam-Filtered: true
16, 33 -- X-IronPort-Anti-Spam-Result: ApoEAGO5yUrRauUp/2dsb2JhbADFHgEJhRGISwKCSIFgBIFS
16, 33 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
16, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Oct 2009 11:17:29 -0500
16, 33 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
16, 33 -- Mon, 5 Oct 2009 11:17:28 -0500
16, 33 -- Received: from um-email05.um.umsystem.edu ([169.254.1.45]) by
16, 33 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Mon, 5 Oct 2009
16, 33 -- 11:17:28 -0500
16, 33 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
16, 33 -- To: "paul_hazelton-at-umanitoba.ca" {paul_hazelton-at-umanitoba.ca} ,
16, 33 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
16, 33 -- Date: Mon, 5 Oct 2009 11:17:27 -0500
16, 33 -- Subject: RE: [Microscopy] Magnification Calibration of Digital Systems
16, 33 -- Thread-Topic: [Microscopy] Magnification Calibration of Digital Systems
16, 33 -- Thread-Index: AcpF1Bh9GIpda0RMT4GPrB8DxMP97wAAtTuA
16, 33 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF07E0391486-at-UM-EMAIL05.um.umsystem.edu}
16, 33 -- References: {200910051554.n95Fs5TD025021-at-ns.microscopy.com}
16, 33 -- In-Reply-To: {200910051554.n95Fs5TD025021-at-ns.microscopy.com}
16, 33 -- Accept-Language: en-US
16, 33 -- Content-Language: en-US
16, 33 -- X-MS-Has-Attach:
16, 33 -- X-MS-TNEF-Correlator:
16, 33 -- acceptlanguage: en-US
16, 33 -- Content-Type: text/plain; charset="us-ascii"
16, 33 -- MIME-Version: 1.0
16, 33 -- X-OriginalArrivalTime: 05 Oct 2009 16:17:28.0884 (UTC) FILETIME=[55897F40:01CA45D7]
16, 33 -- Content-Transfer-Encoding: 8bit
16, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n95GHZeP006735
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Mon, 5 Oct 2009 11:29:31 -0500
Subject: [Microscopy] clarification - magnification calibration of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry, should have said the system. this is for a TEM, not a light
microscope.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
5, 18 -- From paul_hazelton-at-umanitoba.ca Mon Oct 5 11:29:31 2009
5, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95GTTfd021437
5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:29:31 -0500
5, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
5, 18 -- (authenticated bits=0)
5, 18 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n95GTRgh026130;
5, 18 -- Mon, 5 Oct 2009 11:29:28 -0500 (CDT)
5, 18 -- Message-ID: {4ACA1EE5.1000300-at-umanitoba.ca}
5, 18 -- Date: Mon, 05 Oct 2009 11:29:25 -0500
5, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 18 -- Subject: clarification - magnification calibration of TEM
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
5, 18 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Mon, 5 Oct 2009 11:42:29 -0500
Subject: [Microscopy] SCSMM meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul

you don't say whether your digital camera is attached to a light
microscope (stereo, transmitted or reflective light) or an electron
microscope (SEM or TEM).

If it's a standard transmitted light compound microscope with 4-6
objectives then it's probably just a case of taking a few comparative
shots of a standard optical micrometer slide and comparing dimensions
for film and digital at fixed enlargements. Hopefully a simple
correction factor could be applied for all your objectives.

I apologise if this is not what you wanted.

Malcolm

Malcolm Haswell
Electron Microscope Unit
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK

email: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: paul_hazelton-at-umanitoba.ca

Announcement:

The regular Fall meeting of the Southern California Society for
Microscopy and Microanalysis (SCSMM) is scheduled for Thursday,
October 22nd, 2009 in San Pedro, CA.
The meeting is open to non-members as well. For more information
visit the SCSMM web site: http://www.scsmm.org/NextMeet.htm

Thank you for your interest,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

phone 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu

==============================Original Headers==============================
5, 19 -- From bozhilov-at-ucr.edu Mon Oct 5 11:42:29 2009
5, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.214.80])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95GgSec018038
5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:42:28 -0500
5, 19 -- Received: from [138.23.185.168] ([138.23.185.168])
5, 19 -- by sentoku.ucr.edu (MOS 3.10.5-GA)
5, 19 -- with ESMTP id JPK12298
5, 19 -- for {microscopy-at-microscopy.com} ;
5, 19 -- Mon, 5 Oct 2009 09:42:26 -0700 (PDT)
5, 19 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
5, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- Subject: SCSMM meeting
5, 19 -- Date: Mon, 5 Oct 2009 09:42:25 -0700
5, 19 -- Message-Id: {BDF1FB1A-F23E-4798-8563-AA4D6D18B170-at-ucr.edu}
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- Mime-Version: 1.0 (Apple Message framework v1076)
5, 19 -- X-Mailer: Apple Mail (2.1076)
5, 19 -- X-Junkmail-Status: score=0/50, host=
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Mon, 5 Oct 2009 11:58:54 -0500
Subject: [Microscopy] Cryo-SEM in N. Calif. ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

A friend of mine needs to find a cryo-SEM to finish a project on plant
shoot apices.

She is located in northern California and would like to find something
as close as possible.

Any suggestions or offers?

Thanks

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
10, 37 -- From jkrupp-at-deltacollege.edu Mon Oct 5 11:58:53 2009
10, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
10, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n95GwqlF000366
10, 37 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:58:53 -0500
10, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
10, 37 -- by localhost (Email Security Appliance) with SMTP id 18B0618C132_ACA25CBB
10, 37 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 16:58:51 +0000 (GMT)
10, 37 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
10, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 0B0E618B0EB_ACA25CBF
10, 37 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 16:58:51 +0000 (GMT)
10, 37 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
10, 37 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
10, 37 -- with ESMTP id 49693327 for microscopy-at-microscopy.com; Mon, 05 Oct 2009 09:58:50 -0700
10, 37 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by
10, 37 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
10, 37 -- ESMTP id KR1WI200.2N7 for {microscopy-at-microscopy.com} ; Mon, 5
10, 37 -- Oct 2009 09:58:50 -0700
10, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1])
10, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id B3D077A384B1
10, 37 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 09:58:50 -0700 (PDT)
10, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu
10, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
10, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
10, 37 -- with ESMTP id QtfPOXMZjsYu for {microscopy-at-microscopy.com} ;
10, 37 -- Mon, 5 Oct 2009 09:58:50 -0700 (PDT)
10, 37 -- Received: from [172.20.3.214] (unknown [172.20.3.214])
10, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 8DFA87A384A3
10, 37 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 09:58:50 -0700 (PDT)
10, 37 -- Message-Id: {312E07FC-60BC-46A2-9CA1-CB960EA0AD7B-at-deltacollege.edu}
10, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
10, 37 -- To: microscopy-at-microscopy.com
10, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 37 -- Content-Transfer-Encoding: 7bit
10, 37 -- Mime-Version: 1.0 (Apple Message framework v936)
10, 37 -- Subject: Cryo-SEM in N. Calif. ?
10, 37 -- Date: Mon, 5 Oct 2009 09:58:50 -0700
10, 37 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Mon, 5 Oct 2009 13:05:23 -0500
Subject: [Microscopy] clarification - magnification calibration of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,
It doesn't matter what type of microscope. The same goes with a TEM. You
use the procedure discussed by Thomas Phillips in his reply to you. Get a
calibration standard and find out the number of pixels per length. Then if
you want a certain length scale marker, multiply that by the pixels per
length value to get the length in pixels of your scale marker and label it
accordingly. The TEM magnification sample that I highly recommend is the
Mag-i-cal sample that John McCaffrey developed. It is the only calibration
sample that works from the very lowest mags to the highest mags on your
microscope. Although you may not need it, you can also calibrate the
rotation calibration and the camera constant for diffraction with this
sample. You can buy it from a number of places including SBT.

What I do is to write a bunch of scale markers for each magnification in the
microscope and keep them as layers in Photoshop in one file. I do the same
for optical microscopes, too. I just make sure that the pixel resolution in
that file is the same as that of the microscope that took the image. Then I
just open the scale marker file and drag the appropriate layer from that
file onto the image from the microscope. Then I flatten the image. Then
you can rescale the image, change its resolution or whatever and the scale
marker is changed along with the image.

Disclaimer: SBT sells the Mag-i-cal(TM) calibration sample for TEM
calibration.


-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com

-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Monday, October 05, 2009 9:36 AM
To: swalck-at-southbaytech.com

Sorry, should have said the system. this is for a TEM, not a light
microscope.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
5, 18 -- From paul_hazelton-at-umanitoba.ca Mon Oct 5 11:29:31 2009
5, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca
[130.179.16.34])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n95GTTfd021437
5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:29:31
-0500
5, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca
[140.193.25.69])
5, 18 -- (authenticated bits=0)
5, 18 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id
n95GTRgh026130;
5, 18 -- Mon, 5 Oct 2009 11:29:28 -0500 (CDT)
5, 18 -- Message-ID: {4ACA1EE5.1000300-at-umanitoba.ca}
5, 18 -- Date: Mon, 05 Oct 2009 11:29:25 -0500
5, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 18 -- MIME-Version: 1.0
5, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 18 -- Subject: clarification - magnification calibration of TEM
5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 18 -- Content-Transfer-Encoding: 7bit
5, 18 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




==============================Original Headers==============================
18, 23 -- From swalck-at-southbaytech.com Mon Oct 5 13:05:23 2009
18, 23 -- Received: from relay01.pair.com (relay01.pair.com [209.68.5.15])
18, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n95I5M7e017252
18, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 13:05:22 -0500
18, 23 -- Received: (qmail 61391 invoked from network); 5 Oct 2009 18:05:21 -0000
18, 23 -- Received: from 99.154.21.201 (HELO SWALCKD1) (99.154.21.201)
18, 23 -- by relay01.pair.com with SMTP; 5 Oct 2009 18:05:21 -0000
18, 23 -- X-pair-Authenticated: 99.154.21.201
18, 23 -- From: "Scott Walck" {swalck-at-southbaytech.com}
18, 23 -- To: {paul_hazelton-at-umanitoba.ca} , {Microscopy-at-microscopy.com}
18, 23 -- References: {200910051635.n95GZejp002379-at-ns.microscopy.com}
18, 23 -- In-Reply-To: {200910051635.n95GZejp002379-at-ns.microscopy.com}
18, 23 -- Subject: RE: [Microscopy] clarification - magnification calibration of TEM
18, 23 -- Date: Mon, 5 Oct 2009 11:05:10 -0700
18, 23 -- Message-ID: {003801ca45e6$61310e80$23932b80$-at-com}
18, 23 -- MIME-Version: 1.0
18, 23 -- Content-Type: text/plain;
18, 23 -- charset="iso-8859-1"
18, 23 -- X-Mailer: Microsoft Office Outlook 12.0
18, 23 -- Thread-Index: AcpF2d/kkODJVTajR3ul/NTC2bwY1AACs+YQ
18, 23 -- Content-language: en-us
18, 23 -- Content-Transfer-Encoding: 8bit
18, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n95I5M7e017252
==============================End of - Headers==============================




From: delannoy-at-jhmi.edu
Date: Mon, 5 Oct 2009 15:53:31 -0500
Subject: [Microscopy] TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listsever,
I'm trying to get a handle on good TEM digital CCD cameras as a replacement of one of the cameras I have on a Phillips CM 120. I'm am interested in quality, but also ease of use (software). What is the consensus out there.

Thank you

Michael Delannoy

==============================Original Headers==============================
3, 28 -- From prvs=delannoy=5229ab223-at-jhmi.edu Mon Oct 5 15:53:31 2009
3, 28 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141])
3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95KrVNe005665
3, 28 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 15:53:31 -0500
3, 28 -- X-IronPort-AV: E=Sophos;i="4.44,508,1249272000";
3, 28 -- d="scan'208";a="255922954"
3, 28 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201])
3, 28 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Oct 2009 16:53:30 -0400
3, 28 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu
3, 28 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006))
3, 28 -- with ESMTP id {0KR200M4C7D88SI0-at-jesmail.johnshopkins.edu} for
3, 28 -- microscopy-at-microscopy.com; Mon, 05 Oct 2009 16:53:32 -0400 (EDT)
3, 28 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.7])
3, 28 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 05 Oct 2009 16:53:32 -0400
3, 28 -- Date: Mon, 05 Oct 2009 16:53:32 -0400
3, 28 -- From: Michael J Delannoy {delannoy-at-jhmi.edu}
3, 28 -- Subject: TEM cameras
3, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} ,
3, 28 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com}
3, 28 -- Message-id: {fc39a1221993.4aca248c-at-johnshopkins.edu}
3, 28 -- MIME-version: 1.0
3, 28 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006)
3, 28 -- Content-type: text/plain; charset=us-ascii
3, 28 -- Content-language: en
3, 28 -- Content-transfer-encoding: 7BIT
3, 28 -- Content-disposition: inline
3, 28 -- X-Accept-Language: en
3, 28 -- Priority: normal
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Mon, 5 Oct 2009 16:19:04 -0500
Subject: [Microscopy] Re: BSE Diodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had success in the past using automotive rear window heater repair paint, it has no
strength so you have to use epoxy to hold the wire firmly in place then the paint to make the
electrical connection.

cheers

rtch

On 4 Oct 2009 at 9:08, dwaugh-at-kent.edu wrote:

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

We have a Amray 1645 with 4 BSE diodes that have broken leads (the
REALLY small gold wires, not the larger ones). These are way too small
to solder without special equipment. The preamp and amp are working
and I would like to move the system to our Amray 1600. Ideally we
would just buy a new detector, but as you all know, budgets are
tight... Does anyone know of some off the shelf diodes that could be
used for a homemade BSE detector. There are lots of small electronic
parts dealers, I'm assuming that some of the diodes they would work
and not be too expensive. Any ideas, Thanks, David



--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
10, 30 -- From r.sims-at-auckland.ac.nz Mon Oct 5 16:19:04 2009
10, 30 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95LJ3eA020739
10, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 16:19:03 -0500
10, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
10, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3DCB09DC29
10, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:19:00 +1300 (NZDT)
10, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
10, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
10, 30 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
10, 30 -- with ESMTP id v3z8uwOBahc2 for {Microscopy-at-microscopy.com} ;
10, 30 -- Tue, 6 Oct 2009 10:19:00 +1300 (NZDT)
10, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
10, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
10, 30 -- (No client certificate requested)
10, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1F1B69DBCB
10, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:19:00 +1300 (NZDT)
10, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
10, 30 -- To: Microscopy-at-microscopy.com
10, 30 -- Date: Tue, 06 Oct 2009 10:22:38 +1300
10, 30 -- MIME-Version: 1.0
10, 30 -- Subject: Re: [Microscopy] BSE Diodes
10, 30 -- Message-ID: {4ACB1A6E.21369.5F7DDC-at-r.sims.auckland.ac.nz}
10, 30 -- Priority: normal
10, 30 -- In-reply-to: {200910032008.n93K8SUt026457-at-ns.microscopy.com}
10, 30 -- References: {200910032008.n93K8SUt026457-at-ns.microscopy.com}
10, 30 -- X-mailer: Pegasus Mail for Windows (4.41)
10, 30 -- Content-type: text/plain; charset=US-ASCII
10, 30 -- Content-transfer-encoding: 7BIT
10, 30 -- Content-description: Mail message body
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Mon, 5 Oct 2009 16:37:51 -0500
Subject: [Microscopy] BSE Diodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just got my Think Geek catalog (gotta love this stuff!), and it has
"wire glue" http://www.thinkgeek.com/gadgets/tools/b70c/
which makes me think I would use either my silver condictive paint or
carbon paint for SEM mounting...

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
4, 20 -- From tina-at-pbrc.hawaii.edu Mon Oct 5 16:37:51 2009
4, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n95LbovG003617
4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 16:37:51 -0500
4, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
4, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n95LbkaM014550
4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:37:47 -1000 (HST)
4, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n95LbjxE014546
4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Oct 2009 11:37:46 -1000 (HST)
4, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
4, 20 -- Date: Mon, 5 Oct 2009 11:37:44 -1000 (HST)
4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
4, 20 -- X-Sender: tina-at-halia
4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
4, 20 -- Subject: Re: [Microscopy] Re: BSE Diodes
4, 20 -- In-Reply-To: {200910052119.n95LJf4k022100-at-ns.microscopy.com}
4, 20 -- Message-ID: {Pine.GSO.4.21.0910051135570.14117-100000-at-halia}
4, 20 -- MIME-Version: 1.0
4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 6 Oct 2009 06:43:18 -0500
Subject: [Microscopy] 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I just wanted to discuss some reflexions I made while discovering the new "5MP color digital camera" from olympus soft.

Let's make a bit of calculation:

1) The best resolution of this camera is 2560 pixels length x 1920 pixels width.
1) Zeiss reports a best resolution for its 100x objective at 0.26 µm (green light)
2) The field of view óf a 100x objective is around 0,20mm (this information was hard to find so I am unsure of it).

Now to simplify the calculations I will ignore that the camera takes only a part of the field of view (I will consider that the diameter of the field of view takes the whole 2500 pixels of the camera)

Let's calculate the pixel size at best camera resolution with a 100x objective:

0,20 mm or 200 µm divided by 2500=0,1 µm. One pixel is thus 0,1 µm in size.

This is more than 2x smaller that the best resolution of this objective! This means that the optics has a lower resolution that the camera.
I conclude that even a camera with half this resolution would be more than enough.

Would you agree with me?

Stéphane

P.S: we are talking here about "classical" microscopes, not the new "4pi" and the likes with super-resolution.





==============================Original Headers==============================
14, 22 -- From nizets2-at-yahoo.com Tue Oct 6 06:43:18 2009
14, 22 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96BhH3T001678
14, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 06:43:17 -0500
14, 22 -- Received: (qmail 28890 invoked by uid 60001); 6 Oct 2009 11:43:17 -0000
14, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1254829397; bh=1KJMuPz7pFLDVfn1fnz6q2xT19zomSmKrM8r4pMFd9w=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=UKH9hvJhZb/wBU2xRIXfMoKxTRuolltJ0QPySofx87SZhFBITGJ+H4zp1xekCp0NVm+8TTXgLt+928gGDJw+g4gl87eQGUsFXLMFDGurRKKnxMn7S3ApFYdOUg8OcnHMF1NP4f4hjA59bTUFYJkeGT6TspMhp1yQnJ9hmuBQIpU=
14, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 22 -- s=s1024; d=yahoo.com;
14, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
14, 22 -- b=w168BoSIkZB68MlmIOcl7uOZfw5/AOkQYZxsVf3tjp/B8ylakLsBJHBNA9zn4h3ilRbwzyN+S1MsbIfqzjCDXWlj7IOVMNwVPUM5V6Py971dzN/QxfXr2GwkrW40sJCPgiMN7j6x4os+D2Ep9xv4lufV/GXsDGtB6MpaDCALXpQ=;
14, 22 -- Message-ID: {240758.28218.qm-at-web110804.mail.gq1.yahoo.com}
14, 22 -- X-YMail-OSG: MDkoTd4VM1l9jApG6ohw4ZqIJaanEhcMvE7YL4v39_1ulMUxz3fNdKz0L34jtXV6zARPKfOc6dOZnX84XWG7.ibYE1pz0dp_92pLLzVGAb6D0le2oF5tARFg0E_cd8.TSwocWGBdw.Z.m26.UEH9Q_NeNYHlLv3IIkuYn0AfKhECiK3kyRoEUUl5e.q.F1bo7o5n4dqqG0nvhvuicB7sYpMslXlohIWGkdfzUYfnXrU99eGh2IvfEqwTo5DS06qWx9nmThKQHuahSSddFOxEWOxqDcXNE1fkfhryzgblrer3EHJG3hH5rdH5L2x2fsdBft3GzZVJ590aOKIqRXmdGHA-
14, 22 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 06 Oct 2009 04:43:16 PDT
14, 22 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.347.3
14, 22 -- Date: Tue, 6 Oct 2009 04:43:16 -0700 (PDT)
14, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
14, 22 -- Subject: 5MP camera in LM: does it make sense?
14, 22 -- To: microscopy-at-microscopy.com
14, 22 -- MIME-Version: 1.0
14, 22 -- Content-Type: text/plain; charset=iso-8859-1
14, 22 -- Content-Transfer-Encoding: 8bit
14, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96BhH3T001678
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Tue, 6 Oct 2009 07:17:27 -0500
Subject: [Microscopy] viaWWW: TEM: Clogged microscope plumbing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david.mitchell-at-emu.usyd.edu.au as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: david.mitchell-at-emu.usyd.edu.au
Name: David Mitchell

Organization: University of Sydney

Title-Subject: [Filtered] TEM: Clogged microscope plumbing

Question: Dear All

Firstly a vote of thanks to all who responded to my recent nickel
grid/immunogold question - very helpful responses.

Secondly, a question regarding a vintage Philips CM12 TEM, which has
shown gradually diminishing cooling water flow. We have backwashed
it, swapped out filters etc, which helped, but it is still not
getting enough cooling, and instability at 120kV may be related to
this issue.

Question: I believe the system may simply be clogged with scale/oxide
crud. I am wondering if we run some cleaning agent through it,
whether this will help. My first thought would be either dilute
acetic acid (white vinegar), or one of those commercial solutions you
see advertised for descaling shower heads, cleaning rust stains from
baths etc. Has anyone done anything like this with any success, and
if so, what did you use?

Thanks and regards,

Dave Mitchell



Login Host: 129.78.64.103
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Tue Oct 6 07:17:26 2009
13, 11 -- Received: from [10.0.0.144] ([130.202.238.72])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96CHO9O017687
13, 11 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 07:17:26 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240805c6f020e24c7e-at-[10.0.0.144]}
13, 11 -- Date: Tue, 6 Oct 2009 07:17:23 +0900
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: TEM: Clogged microscope plumbing
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: donk-at-ardl
Date: Tue, 6 Oct 2009 08:17:09 -0500
Subject: [Microscopy] viaWWW: RE: TEM Clogged Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both donk-at-ardl,com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: donk-at-ardl,com
Name: Don Kierstead

Organization: ARDL

Title-Subject: [Filtered] RE: TEM Clogged Microscope

Question: CLR works fine. Just add to chiller reservior and let it
circulate best it can overnight. Might take a couple of days to
completely clear plumbing. We have a CM12 that had the same problem.
If you have any questions contact Russ Buyham at FEI.

Login Host: 64.179.51.38
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Oct 6 08:17:09 2009
6, 11 -- Received: from [10.0.0.144] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96DH7hM005880
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:17:08 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240807c6f02eda92b0-at-[10.0.0.144]}
6, 11 -- Date: Tue, 6 Oct 2009 08:17:05 +0900
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: donk-at-ardl, com-at-ns.microscopy.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: RE: TEM Clogged Microscope
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 6 Oct 2009 08:28:36 -0500
Subject: [Microscopy] Re: 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thinking along those lines.....
Is green light the best wavelength for the camera, what is the spacing of
green photosensitive elements in the camera?
If the separation between two features is exactly 1 pixel, what happens
when we blow up the image 2X?
And if we need to make a measurement of fine structure and it contains only
two pixels can we make a measurement?

I recently tried an informal experiment: I imaged fine structure with
normal illumination and then with a green filter (sorry no wavelength). I
expected the green illuminated image to be better (again most highly
corrected for green light) The image was worse. My explanation was the
camera contains elements sensitive to three primary colors. The system
collects color images and the computer gray scales it. Using green light I
use only 1/3 of all the possible elements.

The classic studies were done with silver grains with different sensitivity
to wavelength, but every grain would react to exposure to light.

X-from these questions I suspect we want more pixels

stay safe......
Frank




nizets2-at-yahoo.com

10/06/2009 07:52 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
nizets2-at-yahoo.com [Microscopy] 5MP camera in LM: does
it make sense?













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear listers,

I just wanted to discuss some reflexions I made while discovering the new
"5MP color digital camera" from olympus soft.

Let's make a bit of calculation:

1) The best resolution of this camera is 2560 pixels length x 1920 pixels
width.
1) Zeiss reports a best resolution for its 100x objective at 0.26 µm (green
light)
2) The field of view óf a 100x objective is around 0,20mm (this information
was hard to find so I am unsure of it).

Now to simplify the calculations I will ignore that the camera takes only a
part of the field of view (I will consider that the diameter of the field
of view takes the whole 2500 pixels of the camera)

Let's calculate the pixel size at best camera resolution with a 100x
objective:

0,20 mm or 200 µm divided by 2500=0,1 µm. One pixel is thus 0,1 µm in size.

This is more than 2x smaller that the best resolution of this objective!
This means that the optics has a lower resolution that the camera.
I conclude that even a camera with half this resolution would be more than
enough.

Would you agree with me?

Stéphane

P.S: we are talking here about "classical" microscopes, not the new "4pi"
and the likes with super-resolution.





==============================Original
Headers==============================
14, 22 -- From nizets2-at-yahoo.com Tue Oct 6 06:43:18 2009
14, 22 -- Received: from web110804.mail.gq1.yahoo.com
(web110804.mail.gq1.yahoo.com [67.195.13.227])
14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id n96BhH3T001678
14, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009
06:43:17 -0500
14, 22 -- Received: (qmail 28890 invoked by uid 60001); 6 Oct 2009 11:43:17
-0000
14, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=yahoo.com; s=s1024; t=1254829397;
bh=1KJMuPz7pFLDVfn1fnz6q2xT19zomSmKrM8r4pMFd9w=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=UKH9hvJhZb/wBU2xRIXfMoKxTRuolltJ0QPySofx87SZhFBITGJ+H4zp1xekCp0NVm
+8TTXgLt+928gGDJw
+g4gl87eQGUsFXLMFDGurRKKnxMn7S3ApFYdOUg8OcnHMF1NP4f4hjA59bTUFYJkeGT6TspMhp1yQnJ9hmuBQIpU=

14, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 22 -- s=s1024; d=yahoo.com;
14, 22 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;

14, 22 --
b=w168BoSIkZB68MlmIOcl7uOZfw5/AOkQYZxsVf3tjp/B8ylakLsBJHBNA9zn4h3ilRbwzyN
+S1MsbIfqzjCDXWlj7IOVMNwVPUM5V6Py971dzN/QxfXr2GwkrW40sJCPgiMN7j6x4os
+D2Ep9xv4lufV/GXsDGtB6MpaDCALXpQ=;
14, 22 -- Message-ID: {240758.28218.qm-at-web110804.mail.gq1.yahoo.com}
14, 22 -- X-YMail-OSG:
MDkoTd4VM1l9jApG6ohw4ZqIJaanEhcMvE7YL4v39_1ulMUxz3fNdKz0L34jtXV6zARPKfOc6dOZnX84XWG7.ibYE1pz0dp_92pLLzVGAb6D0le2oF5tARFg0E_cd8.TSwocWGBdw.Z.m26.UEH9Q_NeNYHlLv3IIkuYn0AfKhECiK3kyRoEUUl5e.q.F1bo7o5n4dqqG0nvhvuicB7sYpMslXlohIWGkdfzUYfnXrU99eGh2IvfEqwTo5DS06qWx9nmThKQHuahSSddFOxEWOxqDcXNE1fkfhryzgblrer3EHJG3hH5rdH5L2x2fsdBft3GzZVJ590aOKIqRXmdGHA-

14, 22 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com
via HTTP; Tue, 06 Oct 2009 04:43:16 PDT
14, 22 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.347.3
14, 22 -- Date: Tue, 6 Oct 2009 04:43:16 -0700 (PDT)
14, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
14, 22 -- Subject: 5MP camera in LM: does it make sense?
14, 22 -- To: microscopy-at-microscopy.com
14, 22 -- MIME-Version: 1.0
14, 22 -- Content-Type: text/plain; charset=iso-8859-1
14, 22 -- Content-Transfer-Encoding: 8bit
14, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n96BhH3T001678
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************



==============================Original Headers==============================
3, 24 -- From frank_karl-at-lincolnelectric.com Tue Oct 6 08:28:36 2009
3, 24 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96DSaMn022879
3, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:28:36 -0500
3, 24 -- In-Reply-To: {200910061152.n96BqcO3013906-at-ns.microscopy.com}
3, 24 -- Subject: Re: [Microscopy] 5MP camera in LM: does it make sense?
3, 24 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com
3, 24 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
3, 24 -- Message-ID: {OF0B3344EF.3551B2FD-ON85257647.0048A5FA-85257647.0049FD20-at-lincolnelectric.com}
3, 24 -- Date: Tue, 6 Oct 2009 09:28:38 -0400
3, 24 -- From: Frank_Karl-at-lincolnelectric.com
3, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 10/06/2009 09:28:09 AM,
3, 24 -- CD-MIME complete at 10/06/2009 09:28:09 AM,
3, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 10/06/2009 09:28:09 AM,
3, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
3, 24 -- 07, 2008) at 10/06/2009 09:28:09 AM,
3, 24 -- Serialize complete at 10/06/2009 09:28:09 AM
3, 24 -- MIME-Version: 1.0
3, 24 -- Content-Type: text/plain;
3, 24 -- charset="ISO-8859-1"
3, 24 -- Content-Transfer-Encoding: 8bit
3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96DSaMn022879
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Tue, 6 Oct 2009 08:31:10 -0500
Subject: [Microscopy] TEM Digital Calibration - Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That looks like a pretty fair assessment to me.

We have a old Pixera camera on our light microscopes. The recorded field of view is between 850 and 1000 um at 100x depending on the microscope. That's using the 10x objective on our reflected light scope along with a 10x photo-eyepiece. The field of view would be 100 um or less with a 100x objective. That converts to 0.04 um per pixel at full 5-MP resolution. Indeed, that is overkill.

I don't mind oversampling some. But I would not want to pay much premium to get a 5 MP camera if a cheaper one would capture all the information.

Those extra pixels might be helpful at lower magnifications depending on the resolution available with those lenses. I will let you do the calculations for your particulars.

I would look at the issue of sensitivity. More pixels means less light per pixels. The available light is spread around more bins. Of course, you should have the option for binning multiple pixels together to increase the signal and reduce the noise. However, there might be some loss in sensitivity since you have more boundaries and their attendance dead area between pixels. (Hopefully the dead areas got thinner as the pixels got smaller. If so, that may not be an issue.)

Warren Straszheim

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, October 06, 2009 6:44 AM
To: wesaia-at-iastate.edu

To all who answered. Much Thanks. It appears that what I have to do is
essentially what I have always done for film, with minor modifications.
Thought that would be the case, but also felt it would be best to poll
those who are doing it already.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


==============================Original Headers==============================
4, 18 -- From paul_hazelton-at-umanitoba.ca Tue Oct 6 08:31:10 2009
4, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96DV9OR028688
4, 18 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:31:09 -0500
4, 18 -- Received: from [192.168.100.100] (wnpgmb014sw-ad02-118-116.dynamic.mts.net [207.161.118.116] (may be forged))
4, 18 -- (authenticated bits=0)
4, 18 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n96DV8EZ028281;
4, 18 -- Tue, 6 Oct 2009 08:31:08 -0500 (CDT)
4, 18 -- Message-ID: {4ACB4696.1020700-at-umanitoba.ca}
4, 18 -- Date: Tue, 06 Oct 2009 08:31:02 -0500
4, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
4, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
4, 18 -- MIME-Version: 1.0
4, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
4, 18 -- Subject: TEM Digital Calibration - Thanks
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
4, 18 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 6 Oct 2009 08:35:31 -0500
Subject: [Microscopy] Re: viaWWW: TEM: Clogged microscope plumbing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi David,

We've had good luck with a commercial product known as "CLR"
(Calcium, Lime, Rust) sold by Jelmar. In the US, we can find this
stuff in our local hardware stores. It contains lactic and gluconic
acids and surfactants. I'm sure you have something similar down under.

We dump it into the reservoir of our water chiller and let it run for
several hours (up to overnight) and then flush the system several
times. We refill with distilled water and add a bit of Chloramine T
and some sodium bicarbonate to adjust the pH (very slightly basic).

Also, I maintain a 1 um cartridge filter immediately before the water
enters the microscope to catch any algae floating through the
system. Most of the filter housings here are clear so that you can
see the filter. I would enclose the filter in a black trash bag to
minimize light exposure. Keeping the entire water system dark also
helps reduce the growth of algae.

As I was finishing this message, I just saw Don Kierstead's
recommendation of CLR too!

Good Luck,
Henk


At 08:18 AM 10/06/09, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
13, 26 -- From colijn.1-at-osu.edu Tue Oct 6 08:35:31 2009
13, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96DZVuf012671
13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:35:31 -0500
13, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
13, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
13, 26 -- id {01NEKC75UPPC964K6C-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
13, 26 -- Tue, 06 Oct 2009 09:35:30 -0400 (EDT)
13, 26 -- Received: from HOC1.ecr6.ohio-state.edu
13, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
13, 26 -- (PMDF V6.3-x18 #31556)
13, 26 -- with ESMTPA id {01NEKC74D2WQ95VJQD-at-ecr6.ohio-state.edu} ; Tue,
13, 26 -- 06 Oct 2009 09:35:28 -0400 (EDT)
13, 26 -- Date: Tue, 06 Oct 2009 09:37:49 -0400
13, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
13, 26 -- Subject: Re: [Microscopy] viaWWW: TEM: Clogged microscope plumbing
13, 26 -- In-reply-to: {200910061218.n96CIVYx019777-at-ns.microscopy.com}
13, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
13, 26 -- To: david.mitchell-at-emu.usyd.edu.au
13, 26 -- Cc: Microscopy-at-microscopy.com
13, 26 -- Message-id: {01NEKC74FM9O95VJQD-at-ecr6.ohio-state.edu}
13, 26 -- MIME-version: 1.0
13, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
13, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
13, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
13, 26 -- References: {200910061218.n96CIVYx019777-at-ns.microscopy.com}
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Tue, 6 Oct 2009 08:36:49 -0500
Subject: [Microscopy] Re: viaWWW: RE: TEM Clogged Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had similar problems and tried radiator flush, CLR, dilute acetic acid
and had little luck. I bought five gals of a commercial phosphoric acid
based detergent and ran a diluted solution through the TEM with a pump and
2 gal tank. That worked and then I flushed the lines until the pH paper
said the acid was gone and then I did a little more.

I never had trouble again, the microscope ran cool , no beam problems or
shut-off.

I also had an in-line filter screen I pulled out and cleaned and replaced.
That was gunky (a highly technical term...) and was restricting the water
flow.

Frank



donk-at-ardl

10/06/2009 09:24 To
AM frank_karl-at-lincolnelectric.com
cc

Please respond to Subject
donk-at-ardl [Microscopy] viaWWW: RE: TEM
Clogged Microscope













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both donk-at-ardl,com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: donk-at-ardl,com
Name: Don Kierstead

Organization: ARDL

Title-Subject: [Filtered] RE: TEM Clogged Microscope

Question: CLR works fine. Just add to chiller reservior and let it
circulate best it can overnight. Might take a couple of days to
completely clear plumbing. We have a CM12 that had the same problem.
If you have any questions contact Russ Buyham at FEI.

Login Host: 64.179.51.38
---------------------------------------------------------------------------

==============================Original
Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Oct 6 08:17:09 2009
6, 11 -- Received: from [10.0.0.144] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n96DH7hM005880
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009
08:17:08 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240807c6f02eda92b0-at-[10.0.0.144]}
6, 11 -- Date: Tue, 6 Oct 2009 08:17:05 +0900
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: donk-at-ardl, com-at-ns.microscopy.com (by way of
MicroscopyListserver)
6, 11 -- Subject: viaWWW: RE: TEM Clogged Microscope
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
25, 22 -- From frank_karl-at-lincolnelectric.com Tue Oct 6 08:36:49 2009
25, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
25, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96DamFj017499
25, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:36:49 -0500
25, 22 -- In-Reply-To: {200910061324.n96DO8p8019206-at-ns.microscopy.com}
25, 22 -- Subject: Re: [Microscopy] viaWWW: RE: TEM Clogged Microscope
25, 22 -- To: donk%ardl-at-lincolnelectric.com, Microscopy-at-microscopy.com
25, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
25, 22 -- Message-ID: {OF6610F776.A4EB3673-ON85257647.004A1C81-85257647.004AC19B-at-lincolnelectric.com}
25, 22 -- Date: Tue, 6 Oct 2009 09:37:01 -0400
25, 22 -- From: Frank_Karl-at-lincolnelectric.com
25, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 10/06/2009 09:36:32 AM,
25, 22 -- CD-MIME complete at 10/06/2009 09:36:32 AM,
25, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 10/06/2009 09:36:32 AM,
25, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
25, 22 -- 07, 2008) at 10/06/2009 09:36:32 AM,
25, 22 -- Serialize complete at 10/06/2009 09:36:32 AM
25, 22 -- MIME-Version: 1.0
25, 22 -- Content-Type: text/plain;
25, 22 -- charset="US-ASCII"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 6 Oct 2009 08:52:59 -0500
Subject: [Microscopy] 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you for your helpful comment and for correcting me with the fact that one needs 2 pixels to get contrast!
The given resolution for the optics at 0,26µm is really optimal. I think that in practice most of us probably don't reach it.
Actually I was fair in my calculations because (1) the camera does not span the entire field of view (2) the optics is not always optimized - this is the difference between theory and practice, if you already noticed it (3) the "real" resolution (of a labeling for example) is probably lower because of technical contraints (the methodolody introduces errors).

Now some of you say that the resolution of the camera makes sense at lower objective powers: 10x or 4x.
I did the calculations:

field of view: 2,5 mm or 2500 µm at best
That means 1 pixel is 1 µm. This is approx. the resolution of the optics.
Now you have to agree that one does not image at 10x in order to push up the resolution. At this mag power one does not really care about the resolution, it is more to have a large field of view. If you want to resolve objects approx. 1µm big, you don't do it at 10x, you do it at 63x or 100x!!!  It may be relevant if you plan to project a 10x image on a 2mx2m white wall, which is not very common. Otherwise I doubt that the eyes would see a difference, or that scientific information would be gained.
Which means that the gain at 10x is not relevant for me neither.

As for the explanation of Scott with regard to RGB pixels, I didn't quite get it.

regards,

Stephane


----- Original Message ----
X-from: "Whittaker, Scott" {WHITTAKS-at-si.edu}
To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com}
Sent: Tuesday, October 6, 2009 3:06:47 PM

Dear listers,

I just wanted to discuss some reflexions I made while discovering the new "5MP color digital camera" from olympus soft.

Let's make a bit of calculation:

1) The best resolution of this camera is 2560 pixels length x 1920 pixels width.
1) Zeiss reports a best resolution for its 100x objective at 0.26 µm (green light)
2) The field of view óf a 100x objective is around 0,20mm (this information was hard to find so I am unsure of it).

Now to simplify the calculations I will ignore that the camera takes only a part of the field of view (I will consider that the diameter of the field of view takes the whole 2500 pixels of the camera)

Let's calculate the pixel size at best camera resolution with a 100x objective:

0,20 mm or 200 µm divided by 2500=0,1 µm. One pixel is thus 0,1 µm in size.

This is more than 2x smaller that the best resolution of this objective! This means that the optics has a lower resolution that the camera.
I conclude that even a camera with half this resolution would be more than enough.

Would you agree with me?

Stéphane

P.S: we are talking here about "classical" microscopes, not the new "4pi" and the likes with super-resolution.


     


==============================Original Headers==============================
14, 22 -- From nizets2-at-yahoo.com Tue Oct  6 06:43:18 2009
14, 22 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
14, 22 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96BhH3T001678
14, 22 --     for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 06:43:17 -0500
14, 22 -- Received: (qmail 28890 invoked by uid 60001); 6 Oct 2009 11:43:17 -0000
14, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1254829397; bh=1KJMuPz7pFLDVfn1fnz6q2xT19zomSmKrM8r4pMFd9w=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=UKH9hvJhZb/wBU2xRIXfMoKxTRuolltJ0QPySofx87SZhFBITGJ+H4zp1xekCp0NVm+8TTXgLt+928gGDJw+g4gl87eQGUsFXLMFDGurRKKnxMn7S3ApFYdOUg8OcnHMF1NP4f4hjA59bTUFYJkeGT6TspMhp1yQnJ9hmuBQIpU=
14, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
14, 22 --  s=s1024; d=yahoo.com;
14, 22 --  h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
14, 22 --  b=w168BoSIkZB68MlmIOcl7uOZfw5/AOkQYZxsVf3tjp/B8ylakLsBJHBNA9zn4h3ilRbwzyN+S1MsbIfqzjCDXWlj7IOVMNwVPUM5V6Py971dzN/QxfXr2GwkrW40sJCPgiMN7j6x4os+D2Ep9xv4lufV/GXsDGtB6MpaDCALXpQ=;
14, 22 -- Message-ID: {240758.28218.qm-at-web110804.mail.gq1.yahoo.com}
14, 22 -- X-YMail-OSG: MDkoTd4VM1l9jApG6ohw4ZqIJaanEhcMvE7YL4v39_1ulMUxz3fNdKz0L34jtXV6zARPKfOc6dOZnX84XWG7.ibYE1pz0dp_92pLLzVGAb6D0le2oF5tARFg0E_cd8.TSwocWGBdw.Z.m26.UEH9Q_NeNYHlLv3IIkuYn0AfKhECiK3kyRoEUUl5e.q.F1bo7o5n4dqqG0nvhvuicB7sYpMslXlohIWGkdfzUYfnXrU99eGh2IvfEqwTo5DS06qWx9nmThKQHuahSSddFOxEWOxqDcXNE1fkfhryzgblrer3EHJG3hH5rdH5L2x2fsdBft3GzZVJ590aOKIqRXmdGHA-
14, 22 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 06 Oct 2009 04:43:16 PDT
14, 22 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.347.3
14, 22 -- Date: Tue, 6 Oct 2009 04:43:16 -0700 (PDT)
14, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
14, 22 -- Subject: 5MP camera in LM: does it make sense?
14, 22 -- To: microscopy-at-microscopy.com
14, 22 -- MIME-Version: 1.0
14, 22 -- Content-Type: text/plain; charset=iso-8859-1
14, 22 -- Content-Transfer-Encoding: 8bit
14, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96BhH3T001678
==============================End of - Headers==============================






==============================Original Headers==============================
39, 24 -- From nizets2-at-yahoo.com Tue Oct 6 08:52:58 2009
39, 24 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227])
39, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96DqwEr001723
39, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:52:58 -0500
39, 24 -- Received: (qmail 99907 invoked by uid 60001); 6 Oct 2009 13:52:58 -0000
39, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1254837177; bh=66mwu0FE/3zMpGYqjce9KLux3Yq5TPOGR0KZmnPnfsI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=4TkZufUekRWf74HrsYZvAUjMJ6jTg2VWStwqw0jiyNn2rK8fHzLUzdEmlIeCkuFb8sjA/qeJVP/XLBckJZKMUHJIaBDP2C/YD9uV90Njt2JDXG1DLwFZdKrPR6lv5JW9vnSyTYzjcFO1YYl0yTTKTYcF4U4BlvTZ0QTGZ455toY=
39, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
39, 24 -- s=s1024; d=yahoo.com;
39, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
39, 24 -- b=BpGszw+S//qzNRstRj3RD3Tvd36nQwt/ZVvkmBGr5r78FI/wOoWb3ZFivkR23gMMR//Du6YRrjWjRzFs9bVhbaNNZV3CWsd4DCKMmI3lD7wtVJqAvVgu3tRJgPuMipZUQG/maP/GbjF+8djz4rsQL7yIi9W3oSTv0iHXiepq4N0=;
39, 24 -- Message-ID: {979626.99329.qm-at-web110804.mail.gq1.yahoo.com}
39, 24 -- X-YMail-OSG: R6_d4FcVM1lcZ1FCP0qyKPZWkrO6Oznhu.sMOnbsmmHSMl3bJ6F0cEoqot8BS97DsyBlrYp8i4iy9YhZlBlIQ2OczH7JU1qLxe1mkgyliQLlJqxAMWtlE4T_xjqnW3dKh2vwygfsH1lJXEuaAdFLTvIaC3SGC4a2Sh1YPfhJxl4XWOKC4X18tZ13yc6MtN1QHnAYMFtmvs1wjwIA9V9_I5fY_70xeihLU5mgYzB_xXMlxY4ABSf8LkTT38vAnp8zgAZiwu9ySqfNaExAdTGV2ObpfvIOZHKnNDTj40OSak9PwJI8Be11mOLar95nboAHAvU-
39, 24 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 06 Oct 2009 06:52:57 PDT
39, 24 -- X-Mailer: YahooMailRC/157.18 YahooMailWebService/0.7.347.3
39, 24 -- References: {200910061145.n96BjW9J004024-at-ns.microscopy.com} {038C04650A93A14381E944A473695F618C6B074B6D-at-SI-MSEV03.US.SINET.SI.EDU}
39, 24 -- Date: Tue, 6 Oct 2009 06:52:57 -0700 (PDT)
39, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
39, 24 -- Subject: Re: [Microscopy] 5MP camera in LM: does it make sense?
39, 24 -- To: microscopy-at-microscopy.com
39, 24 -- In-Reply-To: {038C04650A93A14381E944A473695F618C6B074B6D-at-SI-MSEV03.US.SINET.SI.EDU}
39, 24 -- MIME-Version: 1.0
39, 24 -- Content-Type: text/plain; charset=iso-8859-1
39, 24 -- Content-Transfer-Encoding: 8bit
39, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96DqwEr001723
==============================End of - Headers==============================




From: truskt-at-musc.edu
Date: Tue, 6 Oct 2009 09:01:01 -0500
Subject: [Microscopy] Re: 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stéphane:
I think your reasoning is good but there are some considerations missing.
A definition of ressolving power states that ressolution is the
minimum distance between two points that can be seen as two separate
ones.
So you shold need three pixels two for each point and one for the
separation (Let's say two dark pixels separated by a clear one)
If each pixel is 0,1 um the ressolution would be 0,3 um or compatible
with the optical ressolution stated.
Just my 0,02
Regards.
kiss

Francisco José Kiss
Phisical Metalurgy Lab.
Federal University of Rio Grande do Sul
Brazil
kiss-at-demet.ufrgs.br

---------- Original Message -----------
X-from: nizets2-at-yahoo.com
To: kiss-at-demet.ufrgs.br
Sent: Tue, 6 Oct 2009 06:53:24 -0500

There's a nice little JAVA app at NIKON's microscopy website that may help in the discussion:
http://www.microscopyu.com/tutorials/java/digitalimaging/pixelcalculator

If you push the magnification to the highest levels, the best resolution needed is 5 megapixels.
Mind you, that's for a monocolor CCD 1" in diameter, where every pixel on the CCD contributes to the image.
The Bayer Filter used in nearly every color-sensitive digital camera spaces out the r, g, and b pixels.

Thomas C. Trusk, PhD
Josh Spruill Imaging Facility
Regenerative Medicine and Cell Biology
MUSC





==============================Original Headers==============================
7, 37 -- From truskt-at-musc.edu Tue Oct 6 09:01:01 2009
7, 37 -- Received: from ironp1.musc.edu (ironp1.musc.edu [128.23.34.114])
7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96E10Xs024284
7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 09:01:01 -0500
7, 37 -- X-IronPort-Anti-Spam-Filtered: true
7, 37 -- X-IronPort-Anti-Spam-Result: ApoEAALqykqAF8sJ/2dsb2JhbADEZIYoiEuEKgQ
7, 37 -- X-IronPort-AV: E=Sophos;i="4.44,513,1249272000";
7, 37 -- d="scan'208";a="508595644"
7, 37 -- Received: from exg-hub.musc.edu (exg-hub3.mdc.musc.edu [128.23.1.188])
7, 37 -- by revere5b.musc.edu (8.13.5/8.13.5) with ESMTP id n96E0WoJ018401
7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:00:32 -0400
7, 37 -- Received: from EVS4.clinlan.local ([192.168.3.2]) by EXG-HUB3.clinlan.local
7, 37 -- ([fe80::449a:6ab2:a531:e296%12]) with mapi; Tue, 6 Oct 2009 10:00:59 -0400
7, 37 -- From: "Trusk, Thomas C." {truskt-at-musc.edu}
7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 37 -- Date: Tue, 6 Oct 2009 10:00:57 -0400
7, 37 -- Subject: [Microscopy] Re: 5MP camera in LM: does it make sense?
7, 37 -- Thread-Topic: [Microscopy] Re: 5MP camera in LM: does it make sense?
7, 37 -- Thread-Index: AcpGiq/RqW6i7M0SST60PbLYYvbpzAAAQJzw
7, 37 -- Message-ID: {784E9E91C8827540A12A901631A7004ACC16331D64-at-EVS4.clinlan.local}
7, 37 -- References: {200910061341.n96DfGFK001420-at-ns.microscopy.com}
7, 37 -- In-Reply-To: {200910061341.n96DfGFK001420-at-ns.microscopy.com}
7, 37 -- Accept-Language: en-US
7, 37 -- Content-Language: en-US
7, 37 -- X-MS-Has-Attach:
7, 37 -- X-MS-TNEF-Correlator:
7, 37 -- x-cr-puzzleid: {BE5BC4D3-2B8C-40E1-B55A-91858D0633A2}
7, 37 -- x-cr-hashedpuzzle: rig= AGTL AOYz B6qB CEbY CQq+ CovP DDcF Dg+H DiRi E1ku
7, 37 -- FrPV F3WV GDsB GcKH
7, 37 -- HRwY;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{BE5BC4D3-2B8C-40E1-B55A-91858D0633A2};dAByAHUAcwBrAHQAQABtAHUAcwBjAC4AZQBkAHUA;Tue,
7, 37 -- 06 Oct 2009 14:00:57
7, 37 -- GMT;WwBNAGkAYwByAG8AcwBjAG8AcAB5AF0AIABSAGUAOgAgADUATQBQACAAYwBhAG0AZQByAGEAIABpAG4AIABMAE0AOgAgAGQAbwBlAHMAIABpAHQAIABtAGEAawBlACAAcwBlAG4AcwBlAD8A
7, 37 -- acceptlanguage: en-US
7, 37 -- Content-Type: text/plain; charset="iso-8859-1"
7, 37 -- MIME-Version: 1.0
7, 37 -- Content-Transfer-Encoding: 8bit
7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96E10Xs024284
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Tue, 6 Oct 2009 10:52:26 -0500
Subject: [Microscopy] viaWWW: RE: TEM Clogged Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I was at Intel, the JEOL service engineers used CLR on our 2010F when
it got 'gunky'. The only warning hey gave was to not leave it in too long
(24 hours max) or it could corrode the copper pipes.

John Mardinly
Western Digital

-----Original Message-----
X-from: donk-at-ardl [mailto:donk-at-ardl]
Sent: Tuesday, October 06, 2009 6:25 AM
To: John Mardinly

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both donk-at-ardl,com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: donk-at-ardl,com
Name: Don Kierstead

Organization: ARDL

Title-Subject: [Filtered] RE: TEM Clogged Microscope

Question: CLR works fine. Just add to chiller reservior and let it
circulate best it can overnight. Might take a couple of days to
completely clear plumbing. We have a CM12 that had the same problem.
If you have any questions contact Russ Buyham at FEI.

Login Host: 64.179.51.38
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Oct 6 08:17:09 2009
6, 11 -- Received: from [10.0.0.144] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n96DH7hM005880
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:17:08
-0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240807c6f02eda92b0-at-[10.0.0.144]}
6, 11 -- Date: Tue, 6 Oct 2009 08:17:05 +0900
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: donk-at-ardl, com-at-ns.microscopy.com (by way of
MicroscopyListserver)
6, 11 -- Subject: viaWWW: RE: TEM Clogged Microscope
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
13, 26 -- From John.Mardinly-at-wdc.com Tue Oct 6 10:52:25 2009
13, 26 -- Received: from wdscexfe02.sc.wdc.com (wdscexfe02.sc.wdc.com [129.253.170.52])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96FqP8Z023210
13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:52:25 -0500
13, 26 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
13, 26 -- Tue, 6 Oct 2009 08:51:25 -0700
13, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 26 -- Content-class: urn:content-classes:message
13, 26 -- MIME-Version: 1.0
13, 26 -- Content-Type: text/plain;
13, 26 -- charset="us-ascii"
13, 26 -- Subject: RE: [Microscopy] viaWWW: RE: TEM Clogged Microscope
13, 26 -- Date: Tue, 6 Oct 2009 08:51:25 -0700
13, 26 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E1184F-at-wdksjexbe01.msj.wdc.com}
13, 26 -- In-Reply-To: {200910061325.n96DPKh8021881-at-ns.microscopy.com}
13, 26 -- X-MS-Has-Attach:
13, 26 -- X-MS-TNEF-Correlator:
13, 26 -- Thread-Topic: [Microscopy] viaWWW: RE: TEM Clogged Microscope
13, 26 -- Thread-Index: AcpGiFImc+IQWpemTuCGlqTHRVxKSgAFDFUg
13, 26 -- References: {200910061325.n96DPKh8021881-at-ns.microscopy.com}
13, 26 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
13, 26 -- To: {donk-at-ardl}
13, 26 -- Cc: {Microscopy-at-microscopy.com}
13, 26 -- X-OriginalArrivalTime: 06 Oct 2009 15:51:25.0628 (UTC) FILETIME=[DC2D4FC0:01CA469C]
13, 26 -- Content-Transfer-Encoding: 8bit
13, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96FqP8Z023210
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Tue, 6 Oct 2009 10:54:45 -0500
Subject: [Microscopy] Re: Re: 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The thing I keep forgetting (possibly because it's so counterintuitive)
is that the *lower* the magnification of the system the *higher* the
pixel count required to capture all the detail. This was first brought
to my attention by a little handbook (I think) put out by Zeiss, but a
quick Google found a similar explanation (without any calculations) from
the Leica website, the page appropriately titled "Beware of Pixel Mania":

http://www.leica-microsystems.com/press-media/our-customer-magazines/resolution-pathology-diagnostics/patgology-diagnostics-resolution-may-2009/beware-of-pixel-mania/

Here's the interesting bit:

"At low magnification, the microscope is usually able to deliver more
details to the camera than it can capture. At high magnification
however, it is the optical system that limits the amount of detail that
a camera can capture. At 1x magnification the instrument delivers about
14.3 megapixels of information to the camera, while at 16x this figure
drops to 2.6 megapixels."

As I recall (and all this is from memory), with 100X oil the number of
pixels required to capture all the information delivered by the optical
system is ridiculously low. So people doing low mag histology, etc. need
the highest pixel count cameras.

Just my 0.02 megapixels worth,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Sent from my {insert this week's hip device}



truskt-at-musc.edu wrote:
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} There's a nice little JAVA app at NIKON's microscopy website that may help in the discussion:
} http://www.microscopyu.com/tutorials/java/digitalimaging/pixelcalculator
}
} If you push the magnification to the highest levels, the best resolution needed is 5 megapixels.
} Mind you, that's for a monocolor CCD 1" in diameter, where every pixel on the CCD contributes to the image.
} The Bayer Filter used in nearly every color-sensitive digital camera spaces out the r, g, and b pixels.
}
} Thomas C. Trusk, PhD
} Josh Spruill Imaging Facility
} Regenerative Medicine and Cell Biology
} MUSC
}
}
}
}
}
} ==============================Original Headers==============================
} 7, 37 -- From truskt-at-musc.edu Tue Oct 6 09:01:01 2009
} 7, 37 -- Received: from ironp1.musc.edu (ironp1.musc.edu [128.23.34.114])
} 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96E10Xs024284
} 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 09:01:01 -0500
} 7, 37 -- X-IronPort-Anti-Spam-Filtered: true
} 7, 37 -- X-IronPort-Anti-Spam-Result: ApoEAALqykqAF8sJ/2dsb2JhbADEZIYoiEuEKgQ
} 7, 37 -- X-IronPort-AV: E=Sophos;i="4.44,513,1249272000";
} 7, 37 -- d="scan'208";a="508595644"
} 7, 37 -- Received: from exg-hub.musc.edu (exg-hub3.mdc.musc.edu [128.23.1.188])
} 7, 37 -- by revere5b.musc.edu (8.13.5/8.13.5) with ESMTP id n96E0WoJ018401
} 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:00:32 -0400
} 7, 37 -- Received: from EVS4.clinlan.local ([192.168.3.2]) by EXG-HUB3.clinlan.local
} 7, 37 -- ([fe80::449a:6ab2:a531:e296%12]) with mapi; Tue, 6 Oct 2009 10:00:59 -0400
} 7, 37 -- From: "Trusk, Thomas C." {truskt-at-musc.edu}
} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 37 -- Date: Tue, 6 Oct 2009 10:00:57 -0400
} 7, 37 -- Subject: [Microscopy] Re: 5MP camera in LM: does it make sense?
} 7, 37 -- Thread-Topic: [Microscopy] Re: 5MP camera in LM: does it make sense?
} 7, 37 -- Thread-Index: AcpGiq/RqW6i7M0SST60PbLYYvbpzAAAQJzw
} 7, 37 -- Message-ID: {784E9E91C8827540A12A901631A7004ACC16331D64-at-EVS4.clinlan.local}
} 7, 37 -- References: {200910061341.n96DfGFK001420-at-ns.microscopy.com}
} 7, 37 -- In-Reply-To: {200910061341.n96DfGFK001420-at-ns.microscopy.com}
} 7, 37 -- Accept-Language: en-US
} 7, 37 -- Content-Language: en-US
} 7, 37 -- X-MS-Has-Attach:
} 7, 37 -- X-MS-TNEF-Correlator:
} 7, 37 -- x-cr-puzzleid: {BE5BC4D3-2B8C-40E1-B55A-91858D0633A2}
} 7, 37 -- x-cr-hashedpuzzle: rig= AGTL AOYz B6qB CEbY CQq+ CovP DDcF Dg+H DiRi E1ku
} 7, 37 -- FrPV F3WV GDsB GcKH
} 7, 37 -- HRwY;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{BE5BC4D3-2B8C-40E1-B55A-91858D0633A2};dAByAHUAcwBrAHQAQABtAHUAcwBjAC4AZQBkAHUA;Tue,
} 7, 37 -- 06 Oct 2009 14:00:57
} 7, 37 -- GMT;WwBNAGkAYwByAG8AcwBjAG8AcAB5AF0AIABSAGUAOgAgADUATQBQACAAYwBhAG0AZQByAGEAIABpAG4AIABMAE0AOgAgAGQAbwBlAHMAIABpAHQAIABtAGEAawBlACAAcwBlAG4AcwBlAD8A
} 7, 37 -- acceptlanguage: en-US
} 7, 37 -- Content-Type: text/plain; charset="iso-8859-1"
} 7, 37 -- MIME-Version: 1.0
} 7, 37 -- Content-Transfer-Encoding: 8bit
} 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96E10Xs024284
} ==============================End of - Headers==============================
} ------------------------------------------------------------------------
}
}
} No virus found in this incoming message.
} Checked by AVG - www.avg.com
} Version: 8.5.420 / Virus Database: 270.14.4/2417 - Release Date: 10/06/09 06:50:00
}
}



==============================Original Headers==============================
16, 20 -- From jehrman-at-mta.ca Tue Oct 6 10:54:45 2009
16, 20 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.205])
16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96FsiZU026357
16, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 10:54:44 -0500
16, 20 -- Received: from host-22-194.mta.ca ([138.73.22.194]:61635)
16, 20 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
16, 20 -- with esmtp id 1MvCNA-0007wL-9v (Exim 4.69)
16, 20 -- (return-path {jehrman-at-mta.ca} ); Tue, 06 Oct 2009 12:54:44 -0300
16, 20 -- Message-ID: {4ACB6837.2060706-at-mta.ca}
16, 20 -- Date: Tue, 06 Oct 2009 12:54:31 -0300
16, 20 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
16, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
16, 20 -- MIME-Version: 1.0
16, 20 -- To: truskt-at-musc.edu, Microscopy Listserv {Microscopy-at-microscopy.com}
16, 20 -- Subject: Re: [Microscopy] Re: 5MP camera in LM: does it make sense?
16, 20 -- References: {200910061401.n96E1Zgq026337-at-ns.microscopy.com}
16, 20 -- In-Reply-To: {200910061401.n96E1Zgq026337-at-ns.microscopy.com}
16, 20 -- X-Enigmail-Version: 0.96.0
16, 20 -- Content-Type: text/plain; charset=ISO-8859-1
16, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: fernandezg-at-missouri.edu
Date: Tue, 6 Oct 2009 11:38:09 -0500
Subject: [Microscopy] Re: 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Since it is NA that determines resolution, you don't necessarily need less pixels at higher mag. For example if the move is from 40x/0.6 to 100x/1.4 the pixel requirement is essentially the same.

-Esteban



--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO 65211

http://www.biotech.missouri.edu/mcc

(573)882-4895
(573)884-9676 fax


} -------------------------------------------
} From: jehrman-at-mta.ca[SMTP:JEHRMAN-at-MTA.CA {mailto:jehrman-at-mta.ca[SMTP:JEHRMAN-at-MTA.CA} ]
} Sent: Tuesday, October 06, 2009 10:59:31 AM
} Subject: [Microscopy] Re: Re: 5MP camera in LM: does it make sense?
}
} The thing I keep forgetting (possibly because it's so counterintuitive)
} is that the *lower* the magnification of the system the *higher* the
} pixel count required to capture all the detail. This was first brought
} to my attention by a little handbook (I think) put out by Zeiss, but a
} quick Google found a similar explanation (without any calculations) from
} the Leica website, the page appropriately titled "Beware of Pixel Mania":
}
} http://www.leica-microsystems.com/press-media/our-customer-magazines/resolution-pathology-diagnostics/patgology-diagnostics-resolution-may-2009/beware-of-pixel-mania/
}
} Here's the interesting bit:
}
} "At low magnification, the microscope is usually able to deliver more
} details to the camera than it can capture. At high magnification
} however, it is the optical system that limits the amount of detail that
} a camera can capture. At 1x magnification the instrument delivers about
} 14.3 megapixels of information to the camera, while at 16x this figure
} drops to 2.6 megapixels."
}
} As I recall (and all this is from memory), with 100X oil the number of
} pixels required to capture all the information delivered by the optical
} system is ridiculously low. So people doing low mag histology, etc. need
} the highest pixel count cameras.
}
} Just my 0.02 megapixels worth,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca {mailto:jehrman-at-mta.ca}
} www: http://www.mta.ca/dmf
}
} Sent from my {insert this week's hip device}
}
}
}
} truskt-at-musc.edu {mailto:truskt-at-musc.edu} wrote:
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } There's a nice little JAVA app at NIKON's microscopy website that may help in the discussion:
} } http://www.microscopyu.com/tutorials/java/digitalimaging/pixelcalculator
} }
} } If you push the magnification to the highest levels, the best resolution needed is 5 megapixels.
} } Mind you, that's for a monocolor CCD 1" in diameter, where every pixel on the CCD contributes to the image.
} } The Bayer Filter used in nearly every color-sensitive digital camera spaces out the r, g, and b pixels.
} }
} } Thomas C. Trusk, PhD
} } Josh Spruill Imaging Facility
} } Regenerative Medicine and Cell Biology
} } MUSC


==============================Original Headers==============================
10, 31 -- From fernandezg-at-missouri.edu Tue Oct 6 11:38:07 2009
10, 31 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96Gc6mv023865
10, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 11:38:07 -0500
10, 31 -- X-IronPort-Anti-Spam-Filtered: true
10, 31 -- X-IronPort-Anti-Spam-Result: ApoEAIMPy0rRauUp/2dsb2JhbADFAwEJhiKISwKCSIFgBIY7
10, 31 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
10, 31 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 06 Oct 2009 11:38:06 -0500
10, 31 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 31 -- Tue, 6 Oct 2009 11:38:06 -0500
10, 31 -- Received: from um-email02.um.umsystem.edu ([169.254.1.99]) by
10, 31 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Tue, 6 Oct 2009
10, 31 -- 11:38:04 -0500
10, 31 -- From: "Fernandez, Gerardo E." {fernandezg-at-missouri.edu}
10, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
10, 31 -- CC: "jehrman-at-mta.ca" {jehrman-at-mta.ca}
10, 31 -- Date: Tue, 6 Oct 2009 11:36:41 -0500
10, 31 -- Subject: Re: 5MP camera in LM: does it make sense?
10, 31 -- Thread-Topic: 5MP camera in LM: does it make sense?
10, 31 -- Thread-Index: AQHKRqMuvO1AM9+k8kWnH+1OtzxCxA==
10, 31 -- Message-ID: {8D0E19AB0675034D81C4AA53C77B733A0911D0AF-at-UM-EMAIL02.um.umsystem.edu}
10, 31 -- Accept-Language: en-US
10, 31 -- Content-Language: en-US
10, 31 -- X-MS-Has-Attach:
10, 31 -- X-MS-TNEF-Correlator:
10, 31 -- acceptlanguage: en-US
10, 31 -- Content-Type: text/plain; charset="iso-8859-1"
10, 31 -- MIME-Version: 1.0
10, 31 -- X-OriginalArrivalTime: 06 Oct 2009 16:38:06.0238 (UTC) FILETIME=[61787BE0:01CA46A3]
10, 31 -- Content-Transfer-Encoding: 8bit
10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96Gc6mv023865
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 6 Oct 2009 12:59:48 -0500
Subject: [Microscopy] 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Bayer filter cuts down on resolution in order to
achieve color capture. Furthermore, typical pixel sizes
are 4-6um.

gary g.


At 06:29 AM 10/6/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
9, 22 -- From gary-at-gaugler.com Tue Oct 6 12:59:48 2009
9, 22 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96Hxl2D012800
9, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 12:59:48 -0500
9, 22 -- Message-Id: {200910061759.n96Hxl2D012800-at-ns.microscopy.com}
9, 22 -- Received: (qmail 18895 invoked from network); 6 Oct 2009 10:42:08 -0700
9, 22 -- Received: by simscan 1.1.0 ppid: 18887, pid: 18888, t: 0.2067s
9, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 22 -- by smtp2 with SMTP; 6 Oct 2009 10:42:08 -0700
9, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 22 -- Date: Tue, 06 Oct 2009 10:59:45 -0700
9, 22 -- To: Frank_Karl-at-lincolnelectric.com
9, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 22 -- Subject: Re: [Microscopy] Re: 5MP camera in LM: does it make sense?
9, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 22 -- In-Reply-To: {200910061329.n96DTirq025373-at-ns.microscopy.com}
9, 22 -- References: {200910061329.n96DTirq025373-at-ns.microscopy.com}
9, 22 -- Mime-Version: 1.0
9, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96Hxl2D012800
==============================End of - Headers==============================




From: michael-at-shaffer.net
Date: Tue, 6 Oct 2009 13:05:49 -0500
Subject: [Microscopy] SEM: elemental sulfur

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As many of you are probably aware, elemental sulphur will evaporate within
the SEM chamber. However we would still like to try using SEM-platformed
image analysis to measure sulphur in metallurgical hydromet processing
by-products.

Does anyone have a good idea of how fast sulphur will evaporate? That is,
during the 1st hour it's difficult to see it going anywhere, but give it a
day in the vacuum and it may be difficult to find. We'd like to think that
less that 10% would disappear in the 1st hour - does anyone have more
experience with it?

We'd also like to think that our ESEM would be designed such that evaporated
sulphur would not pose problems for the electron column ... true?

TIA
~~~~~~~~~~~~~~~~~~~~~~~~~
cheerios, michael shaffer  :o)
SEM-MLA Research Coordinator
INCO Innovation Centre
Memorial University
St. John's Newfoundland
  http://www.mun.ca/creait/maf/






==============================Original Headers==============================
9, 28 -- From michael-at-shaffer.net Tue Oct 6 13:05:48 2009
9, 28 -- Received: from smtprelay.hostedemail.com (smtprelay0020.hostedemail.com [216.40.44.20])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96I5mPT022559
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:05:48 -0500
9, 28 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254])
9, 28 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id C5047C3A275
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:47 +0000 (UTC)
9, 28 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574
9, 28 -- X-Filterd-Recvd-Size: 2054
9, 28 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141])
9, 28 -- (Authenticated sender: michael-at-shaffer.net)
9, 28 -- by omf07.hostedemail.com (Postfix) with ESMTP
9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:45 +0000 (UTC)
9, 28 -- From: "michael shaffer" {michael-at-shaffer.net}
9, 28 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com}
9, 28 -- Subject: SEM: elemental sulfur
9, 28 -- Date: Tue, 6 Oct 2009 15:35:41 -0230
9, 28 -- Message-ID: {003901ca46af$a0e95db0$e2bc1910$-at-net}
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- X-Mailer: Microsoft Office Outlook 12.0
9, 28 -- Thread-Index: AcpGr5ocS33zrYBFRiGQPJNRKu0NFQ==
9, 28 -- Content-Language: en-ca
9, 28 -- x-cr-hashedpuzzle: PoE= AD4Z BRwz BTrI C9Uy Dgr/ DrG9 Dv21 FG4M F1L1 HXxw Is2A JV6w Kd/J LB48 LXHS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{4F43D163-E910-4167-88DC-848BCF8448E3};bQBpAGMAaABhAGUAbABAAHMAaABhAGYAZgBlAHIALgBuAGUAdAA=;Tue, 06 Oct 2009 18:05:35 GMT;UwBFAE0AOgAgAGUAbABlAG0AZQBuAHQAYQBsACAAcwB1AGwAZgB1AHIA
9, 28 -- x-cr-puzzleid: {4F43D163-E910-4167-88DC-848BCF8448E3}
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96I5mPT022559
==============================End of - Headers==============================




From: michael-at-shaffer.net
Date: Tue, 6 Oct 2009 13:28:42 -0500
Subject: [Microscopy] 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Francisco José writes ...
Regarding ...
} Dear Stéphane:
} I think your reasoning is good but there are some considerations missing.
} A definition of ressolving power states that ressolution is the
} minimum distance between two points that can be seen as two separate
} ones.
} So you shold need three pixels two for each point and one for the
} separation (Let's say two dark pixels separated by a clear one)
} If each pixel is 0,1 um the ressolution would be 0,3 um or compatible
} with the optical ressolution stated.

I believe that should be 2 pixels. That is, if you formulate the resolution
question differently ... ie, "How pixels are required to discriminate 100
particles in close proximity?", you would need only 201 pixels, which allows
for your criteria that a pixel be between, but converges on a 2:1 ratio.

The OP's query is legitimate, but there's nothing wrong with using more
pixels than necessary. I also notice that some of the newer cameras can bin
neighboring pixels for reducing noise .. but that may be normal for modern
cameras designed for microscopy.

~~~~~~~~~~~~~~~~~~~~~~~~~
cheerios, michael shaffer  :o)
SEM-MLA Research Coordinator
INCO Innovation Centre
Memorial University
St. John's Newfoundland
  http://www.mun.ca/creait/maf/



} ---------- Original Message -----------
} X-from: nizets2-at-yahoo.com
} To: kiss-at-demet.ufrgs.br
} Sent: Tue, 6 Oct 2009 06:53:24 -0500
} Subject: [Microscopy] 5MP camera in LM: does it make sense?
}
} }
} --------------------------------------------------------------------------
} --
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------------------
} --
} }
} } Dear listers,
} }
} } I just wanted to discuss some reflexions I made while
} } discovering the new "5MP color digital camera" from olympus soft.
} }
} } Let's make a bit of calculation:
} }
} } 1) The best resolution of this camera is 2560 pixels length
} } x 1920 pixels width. 1) Zeiss reports a best resolution for
} } its 100x objective at 0.26 µm (green light) 2) The field of
} } view óf a 100x objective is around 0,20mm (this information
} } was hard to find so I am unsure of it).
} }
} } Now to simplify the calculations I will ignore that the
} } camera takes only a part of the field of view (I will
} } consider that the diameter of the field of view takes the
} } whole 2500 pixels of the camera)
} }
} } Let's calculate the pixel size at best camera resolution
} } with a 100x objective:
} }
} } 0,20 mm or 200 µm divided by 2500=0,1 µm. One pixel is thus
} } 0,1 µm in size.
} }
} } This is more than 2x smaller that the best resolution of
} } this objective! This means that the optics has a
} } lower resolution that the camera. I conclude that even a
} } camera with half this resolution would be more than enough.
} }
} } Would you agree with me?
} }
} } Stéphane
} }
} } P.S: we are talking here about "classical" microscopes, not
} } the new "4pi" and the likes with super-resolution.
} }
} } ==============================Original
} Headers==============================
} } 14, 22 -- From nizets2-at-yahoo.com Tue Oct 6 06:43:18 2009
} } 14, 22 -- Received: from web110804.mail.gq1.yahoo.com
} } (web110804.mail.gq1.yahoo.com [67.195.13.227]) 14, 22 -- by
} } ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96BhH3T001678
} }
} } 14, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009
} } 06:43:17 -0500
} } 14, 22 -- Received: (qmail 28890 invoked by uid 60001); 6
} } Oct 2009 11:43:17 -0000 14, 22 -- DKIM-Signature: v=1; a=rsa-
} } sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024;
} } t=1254829397;
} } bh=1KJMuPz7pFLDVfn1fnz6q2xT19zomSmKrM8r4pMFd9w=; h=Message-
} } ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-
} } Version:Content-Type:Content-Transfer-Encoding;
} b=UKH9hvJhZb/wBU2xRIXfMoKxTRuolltJ0QPySofx87SZhFBITGJ+H4zp1xekCp0NVm+8TTXg
} Lt+928gGDJw+g4gl87eQGUsFXLMFDGurRKKnxMn7S3ApFYdOUg8OcnHMF1NP4f4hjA59bTUFYJ
} keGT6TspMhp1yQnJ9hmuBQIpU=
} } 14, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} } 14, 22 -- s=s1024; d=yahoo.com;
} } 14, 22 -- h=Message-ID:X-YMail-OSG:Received:X-
} } Mailer:Date:From:Subject:To:MIME-Version:Content-
} } Type:Content-Transfer-Encoding; 14, 22 --
} b=w168BoSIkZB68MlmIOcl7uOZfw5/AOkQYZxsVf3tjp/B8ylakLsBJHBNA9zn4h3ilRbwzyN+
} S1MsbIfqzjCDXWlj7IOVMNwVPUM5V6Py971dzN/QxfXr2GwkrW40sJCPgiMN7j6x4os+D2Ep9x
} v4lufV/GXsDGtB6MpaDCALXpQ=;
} } 14, 22 -- Message-ID: {240758.28218.qm-at-web110804.mail.gq1.yahoo.com}
} } 14, 22 -- X-YMail-OSG:
} }
} MDkoTd4VM1l9jApG6ohw4ZqIJaanEhcMvE7YL4v39_1ulMUxz3fNdKz0L34jtXV6zARPKfOc6d
} OZnX84XWG7.ibYE1pz0dp_92pLLzVGAb6D0le2oF5tARFg0E_cd8.TSwocWGBdw.Z.m26.UEH9
} Q_NeNYHlLv3IIkuYn0AfKhECiK3kyRoEUUl5e.q.F1bo7o5n4dqqG0nvhvuicB7sYpMslXlohI
} WGkdfzUYfnXrU99eGh2IvfEqwTo5DS06qWx9nmThKQHuahSSddFOxEWOxqDcXNE1fkfhryzgbl
} rer3EHJG3hH5rdH5L2x2fsdBft3GzZVJ590aOKIqRXmdGHA-
} } 14, 22 -- Received: from [80.122.101.100] by
} } web110804.mail.gq1.yahoo.com via HTTP; Tue, 06 Oct 2009
} } 04:43:16 PDT 14, 22 -- X-Mailer: YahooMailRC/157.18
} YahooMailWebService/0.7.347.3
} } 14, 22 -- Date: Tue, 6 Oct 2009 04:43:16 -0700 (PDT)
} } 14, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} } 14, 22 -- Subject: 5MP camera in LM: does it make sense?
} } 14, 22 -- To: microscopy-at-microscopy.com
} } 14, 22 -- MIME-Version: 1.0
} } 14, 22 -- Content-Type: text/plain; charset=iso-8859-1
} } 14, 22 -- Content-Transfer-Encoding: 8bit
} } 14, 22 -- X-MIME-Autoconverted: from quoted-printable to
} } 8bit by ns.microscopy.com id n96BhH3T001678
} } ==============================End of -
} Headers==============================
} ------- End of Original Message -------
}
}
} ==============================Original
} Headers==============================
} 5, 21 -- From kiss-at-demet.ufrgs.br Tue Oct 6 08:55:17 2009
} 5, 21 -- Received: from servidor.demet.ufrgs.br (servidor.demet.ufrgs.br
} [143.54.39.253])
} 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} n96DtGI3006604
} 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 08:55:16 -
} 0500
} 5, 21 -- Received: from webmail.demet.ufrgs.br (localhost.localdomain
} [127.0.0.1])
} 5, 21 -- by servidor.demet.ufrgs.br (8.13.8/8.12.10) with ESMTP id
} n96DBWAw011753;
} 5, 21 -- Tue, 6 Oct 2009 10:11:32 -0300
} 5, 21 -- From: "Francisco Jose Kiss" {kiss-at-demet.ufrgs.br}
} 5, 21 -- To: microscopy-at-microscopy.com
} 5, 21 -- Cc: nizets2-at-yahoo.com
} 5, 21 -- Subject: Re: [Microscopy] 5MP camera in LM: does it make sense?
} 5, 21 -- Date: Tue, 6 Oct 2009 10:11:32 -0300
} 5, 21 -- Message-Id: {20091006125745.M79416-at-demet.ufrgs.br}
} 5, 21 -- In-Reply-To: {200910061153.n96BrO3T015062-at-ns.microscopy.com}
} 5, 21 -- References: {200910061153.n96BrO3T015062-at-ns.microscopy.com}
} 5, 21 -- X-Mailer: OpenWebMail 2.52 20060502
} 5, 21 -- X-OriginatingIP: 143.54.129.200 (kiss)
} 5, 21 -- MIME-Version: 1.0
} 5, 21 -- Content-Type: text/plain;
} 5, 21 -- charset=iso-8859-1
} 5, 21 -- Received-SPF: pass (servidor.demet.ufrgs.br: domain of
} kiss-at-demet.ufrgs.br designates 127.0.0.1 as permitted sender)
} receiver=servidor.demet.ufrgs.br; client-ip=127.0.0.1;
} helo=webmail.demet.ufrgs.br; envelope-from=kiss-at-demet.ufrgs.br; x-
} software=spfmilter 0.97 http://www.acme.com/software/spfmilter/ with
} libspf2-1.0.0;
} ==============================End of -
} Headers==============================
}
} __________ Information from ESET NOD32 Antivirus, version of virus
} signature database 4484 (20091006) __________
}
} The message was checked by ESET NOD32 Antivirus.
}
} http://www.eset.com
}




==============================Original Headers==============================
10, 29 -- From michael-at-shaffer.net Tue Oct 6 13:28:41 2009
10, 29 -- Received: from smtprelay.hostedemail.com (smtprelay0201.hostedemail.com [216.40.44.201])
10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96ISfPg010841
10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:28:41 -0500
10, 29 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254])
10, 29 -- by smtprelay01.hostedemail.com (Postfix) with SMTP id 4A6941E686C9;
10, 29 -- Tue, 6 Oct 2009 18:28:41 +0000 (UTC)
10, 29 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574
10, 29 -- X-Filterd-Recvd-Size: 9550
10, 29 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141])
10, 29 -- (Authenticated sender: michael-at-shaffer.net)
10, 29 -- by omf05.hostedemail.com (Postfix) with ESMTP;
10, 29 -- Tue, 6 Oct 2009 18:28:39 +0000 (UTC)
10, 29 -- From: "michael shaffer" {michael-at-shaffer.net}
10, 29 -- To: {kiss-at-demet.ufrgs.br}
10, 29 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com}
10, 29 -- References: {200910061355.n96Dtc0H007715-at-ns.microscopy.com}
10, 29 -- In-Reply-To: {200910061355.n96Dtc0H007715-at-ns.microscopy.com}
10, 29 -- Subject: RE: [Microscopy] Re: 5MP camera in LM: does it make sense?
10, 29 -- Date: Tue, 6 Oct 2009 15:58:35 -0230
10, 29 -- Message-ID: {004901ca46b2$d3619070$7a24b150$-at-net}
10, 29 -- MIME-Version: 1.0
10, 29 -- Content-Type: text/plain;
10, 29 -- charset="iso-8859-1"
10, 29 -- X-Mailer: Microsoft Office Outlook 12.0
10, 29 -- Thread-Index: AcpGjK8RAEPq9ieURCusJ892S3WLWgAJNhlA
10, 29 -- Content-Language: en-ca
10, 29 -- Content-Transfer-Encoding: 8bit
10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96ISfPg010841
==============================End of - Headers==============================




From: nyilmaz-at-mersin.edu.tr
Date: Tue, 6 Oct 2009 13:39:54 -0500
Subject: [Microscopy] switching specimen from paraffin to resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Is there any tested and approved protocol recommendation for reembedding of
paraffin embedded tissue in resin? We need the protocol for kidney fine
needle biopsy specimen of a patient fixed in formalin and embedded in
paraffin.
Thanks in advance.

Necat Yilmaz


==============================Original Headers==============================
4, 31 -- From nyilmaz-at-mersin.edu.tr Tue Oct 6 13:39:54 2009
4, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96Idr9N025758
4, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:39:53 -0500
4, 31 -- Received: from localhost (localhost [127.0.0.1])
4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id E9D0F2F73C1
4, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 21:40:07 +0300 (EEST)
4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr
4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1])
4, 31 -- by localhost (mail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024)
4, 31 -- with ESMTP id xJfv3Wx4m5Wk for {Microscopy-at-microscopy.com} ;
4, 31 -- Tue, 6 Oct 2009 21:40:04 +0300 (EEST)
4, 31 -- Received: from nejat1 (unknown [78.187.197.180])
4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 537122F73BC
4, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 21:40:04 +0300 (EEST)
4, 31 -- Message-ID: {001101ca46b4$65aa07e0$2101a8c0-at-nejat1}
4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com}
4, 31 -- Subject: switching specimen from paraffin to resin
4, 31 -- Date: Tue, 6 Oct 2009 21:39:52 +0300
4, 31 -- MIME-Version: 1.0
4, 31 -- Content-Type: text/plain;
4, 31 -- format=flowed;
4, 31 -- charset="iso-8859-9";
4, 31 -- reply-type=original
4, 31 -- Content-Transfer-Encoding: 7bit
4, 31 -- X-Priority: 3
4, 31 -- X-MSMail-Priority: Normal
4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3598
4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr}
4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Tue, 6 Oct 2009 13:57:57 -0500
Subject: [Microscopy] SEM: elemental sulfur

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael,
I think that your best bet would be to find an SEM with a cold stage. You
need to look at the vapor pressure versus temperature value for sulfur.
Then you want to be well below the temperature that corresponds to the vapor
pressure equal to the pressure in the SEM.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net]
Sent: Tuesday, October 06, 2009 11:13 AM
To: swalck-at-southbaytech.com

As many of you are probably aware, elemental sulphur will evaporate within
the SEM chamber. However we would still like to try using SEM-platformed
image analysis to measure sulphur in metallurgical hydromet processing
by-products.

Does anyone have a good idea of how fast sulphur will evaporate? That is,
during the 1st hour it's difficult to see it going anywhere, but give it a
day in the vacuum and it may be difficult to find. We'd like to think that
less that 10% would disappear in the 1st hour - does anyone have more
experience with it?

We'd also like to think that our ESEM would be designed such that evaporated
sulphur would not pose problems for the electron column ... true?

TIA
~~~~~~~~~~~~~~~~~~~~~~~~~
cheerios, michael shaffer  :o)
SEM-MLA Research Coordinator
INCO Innovation Centre
Memorial University
St. John's Newfoundland
  http://www.mun.ca/creait/maf/






==============================Original Headers==============================
9, 28 -- From michael-at-shaffer.net Tue Oct 6 13:05:48 2009
9, 28 -- Received: from smtprelay.hostedemail.com
(smtprelay0020.hostedemail.com [216.40.44.20])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n96I5mPT022559
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:05:48
-0500
9, 28 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254])
9, 28 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id
C5047C3A275
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:47
+0000 (UTC)
9, 28 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574
9, 28 -- X-Filterd-Recvd-Size: 2054
9, 28 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca
[134.153.130.141])
9, 28 -- (Authenticated sender: michael-at-shaffer.net)
9, 28 -- by omf07.hostedemail.com (Postfix) with ESMTP
9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:45
+0000 (UTC)
9, 28 -- From: "michael shaffer" {michael-at-shaffer.net}
9, 28 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com}
9, 28 -- Subject: SEM: elemental sulfur
9, 28 -- Date: Tue, 6 Oct 2009 15:35:41 -0230
9, 28 -- Message-ID: {003901ca46af$a0e95db0$e2bc1910$-at-net}
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- X-Mailer: Microsoft Office Outlook 12.0
9, 28 -- Thread-Index: AcpGr5ocS33zrYBFRiGQPJNRKu0NFQ==
9, 28 -- Content-Language: en-ca
9, 28 -- x-cr-hashedpuzzle: PoE= AD4Z BRwz BTrI C9Uy Dgr/ DrG9 Dv21 FG4M
F1L1 HXxw Is2A JV6w Kd/J LB48
LXHS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;
Sosha1_v1;7;{4F43D163-E910-4167-88DC-848BCF8448E3};bQBpAGMAaABhAGUAbABAAHMAa
ABhAGYAZgBlAHIALgBuAGUAdAA=;Tue, 06 Oct 2009 18:05:35
GMT;UwBFAE0AOgAgAGUAbABlAG0AZQBuAHQAYQBsACAAcwB1AGwAZgB1AHIA
9, 28 -- x-cr-puzzleid: {4F43D163-E910-4167-88DC-848BCF8448E3}
9, 28 -- Content-Transfer-Encoding: 8bit
9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n96I5mPT022559
==============================End of - Headers==============================




==============================Original Headers==============================
20, 24 -- From swalck-at-southbaytech.com Tue Oct 6 13:57:56 2009
20, 24 -- Received: from relay01.pair.com (relay01.pair.com [209.68.5.15])
20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n96IvuWB008571
20, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:57:56 -0500
20, 24 -- Received: (qmail 63667 invoked from network); 6 Oct 2009 18:57:55 -0000
20, 24 -- Received: from 99.154.21.201 (HELO SWALCKD1) (99.154.21.201)
20, 24 -- by relay01.pair.com with SMTP; 6 Oct 2009 18:57:55 -0000
20, 24 -- X-pair-Authenticated: 99.154.21.201
20, 24 -- From: "Scott Walck" {swalck-at-southbaytech.com}
20, 24 -- To: {Microscopy-at-microscopy.com}
20, 24 -- Cc: {michael-at-shaffer.net}
20, 24 -- References: {200910061812.n96ICc8Z008554-at-ns.microscopy.com}
20, 24 -- In-Reply-To: {200910061812.n96ICc8Z008554-at-ns.microscopy.com}
20, 24 -- Subject: RE: [Microscopy] SEM: elemental sulfur
20, 24 -- Date: Tue, 6 Oct 2009 11:57:39 -0700
20, 24 -- Message-ID: {003501ca46b6$e0dad370$a2907a50$-at-com}
20, 24 -- MIME-Version: 1.0
20, 24 -- Content-Type: text/plain;
20, 24 -- charset="iso-8859-1"
20, 24 -- X-Mailer: Microsoft Office Outlook 12.0
20, 24 -- Thread-Index: AcpGsJYa4xBiDP8ZQVmislvWPOn53wABXLFw
20, 24 -- Content-Language: en-us
20, 24 -- Content-Transfer-Encoding: 8bit
20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96IvuWB008571
==============================End of - Headers==============================




From: gw265-at-cam.ac.uk
Date: Tue, 6 Oct 2009 15:19:37 -0500
Subject: [Microscopy] immuno-TEM resin infiltration time?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

For how long can/do you infiltrate LR White resin?

We have had some problems with extremely poor infiltration of LR White
resin into agar-embedded samples of single-celled
green algae. The agar blocks are {1mm squared and are basically like
little blobs of jelly, falling out of the resin
blocks once the resin is cooked. We can and will make the agar blocks much
smaller... but what is the longest
infiltration time we can use with LR white and still preserve
antigenicity? I'm guessing that the (very effective)
practice of infiltrating Spurr's or Epon for 1-3 weeks is not going to be
relevant here if we still want to do
immunogold work at the end of the process.

(Yes, cryo methods would be greatly preferable. No, we don't have the
money or the equipment.)

thanks

Giselle Walker
University of Cambridge



==============================Original Headers==============================
8, 23 -- From gw265-at-hermes.cam.ac.uk Tue Oct 6 15:19:37 2009
8, 23 -- Received: from ppsw-6.csi.cam.ac.uk (ppsw-6.csi.cam.ac.uk [131.111.8.136])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96KJaWM026924
8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 15:19:37 -0500
8, 23 -- X-Cam-AntiVirus: no malware found
8, 23 -- X-Cam-SpamDetails: not scanned
8, 23 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
8, 23 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:58357)
8, 23 -- by ppsw-6.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25)
8, 23 -- with esmtpa (EXTERNAL:gw265) id 1MvGVU-0004jE-Lt (Exim 4.70) for Microscopy-at-microscopy.com
8, 23 -- (return-path {gw265-at-hermes.cam.ac.uk} ); Tue, 06 Oct 2009 21:19:36 +0100
8, 23 -- Received: from gw265 (helo=localhost) by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk)
8, 23 -- with local-esmtp id 1MvGVU-00089t-Ov (Exim 4.67) for Microscopy-at-microscopy.com
8, 23 -- (return-path {gw265-at-hermes.cam.ac.uk} ); Tue, 06 Oct 2009 21:19:36 +0100
8, 23 -- Date: Tue, 6 Oct 2009 21:19:36 +0100 (BST)
8, 23 -- From: Giselle Walker {gw265-at-cam.ac.uk}
8, 23 -- X-X-Sender: gw265-at-hermes-1.csi.cam.ac.uk
8, 23 -- To: Microscopy-at-microscopy.com
8, 23 -- Subject: immuno-TEM resin infiltration time?
8, 23 -- Message-ID: {Pine.LNX.4.64.0910062115570.30944-at-hermes-1.csi.cam.ac.uk}
8, 23 -- MIME-Version: 1.0
8, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
8, 23 -- Sender: "Dr. G. Walker" {gw265-at-hermes.cam.ac.uk}
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Tue, 6 Oct 2009 17:43:52 -0500
Subject: [Microscopy] 5MP camera in LM: does it make sense?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

So wouldn't this be a good time to congratulate Willard Smith and George
Boyle for inventing the CCD, seeing as they just won the Nobel Prize in
Physics today for doing just that?

John Mardinly
Western Digital

-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Tuesday, October 06, 2009 11:09 AM
To: John Mardinly

The Bayer filter cuts down on resolution in order to
achieve color capture. Furthermore, typical pixel sizes
are 4-6um.

gary g.


At 06:29 AM 10/6/2009, you wrote:



} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
9, 22 -- From gary-at-gaugler.com Tue Oct 6 12:59:48 2009
9, 22 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n96Hxl2D012800
9, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 12:59:48
-0500
9, 22 -- Message-Id: {200910061759.n96Hxl2D012800-at-ns.microscopy.com}
9, 22 -- Received: (qmail 18895 invoked from network); 6 Oct 2009 10:42:08
-0700
9, 22 -- Received: by simscan 1.1.0 ppid: 18887, pid: 18888, t: 0.2067s
9, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 22 -- by smtp2 with SMTP; 6 Oct 2009 10:42:08 -0700
9, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 22 -- Date: Tue, 06 Oct 2009 10:59:45 -0700
9, 22 -- To: Frank_Karl-at-lincolnelectric.com
9, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 22 -- Subject: Re: [Microscopy] Re: 5MP camera in LM: does it make sense?
9, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 22 -- In-Reply-To: {200910061329.n96DTirq025373-at-ns.microscopy.com}
9, 22 -- References: {200910061329.n96DTirq025373-at-ns.microscopy.com}
9, 22 -- Mime-Version: 1.0
9, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
9, 22 -- Content-Transfer-Encoding: 8bit
9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n96Hxl2D012800
==============================End of - Headers==============================


==============================Original Headers==============================
17, 26 -- From John.Mardinly-at-wdc.com Tue Oct 6 17:43:52 2009
17, 26 -- Received: from wdscexfe02.sc.wdc.com (wdscexfe02.sc.wdc.com [129.253.170.52])
17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96Mhqdp020576
17, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 17:43:52 -0500
17, 26 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
17, 26 -- Tue, 6 Oct 2009 15:42:52 -0700
17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 26 -- Content-class: urn:content-classes:message
17, 26 -- MIME-Version: 1.0
17, 26 -- Content-Type: text/plain;
17, 26 -- charset="us-ascii"
17, 26 -- Subject: RE: [Microscopy] 5MP camera in LM: does it make sense?
17, 26 -- Date: Tue, 6 Oct 2009 15:42:52 -0700
17, 26 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E11852-at-wdksjexbe01.msj.wdc.com}
17, 26 -- In-Reply-To: {200910061809.n96I9KKm000408-at-ns.microscopy.com}
17, 26 -- X-MS-Has-Attach:
17, 26 -- X-MS-TNEF-Correlator:
17, 26 -- Thread-Topic: [Microscopy] 5MP camera in LM: does it make sense?
17, 26 -- Thread-Index: AcpGr/68Il7HhtZiSn6TYO0LUaUkygAJhRVg
17, 26 -- References: {200910061809.n96I9KKm000408-at-ns.microscopy.com}
17, 26 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
17, 26 -- To: {gary-at-gaugler.com}
17, 26 -- Cc: {Microscopy-at-microscopy.com}
17, 26 -- X-OriginalArrivalTime: 06 Oct 2009 22:42:52.0998 (UTC) FILETIME=[56FF0660:01CA46D6]
17, 26 -- Content-Transfer-Encoding: 8bit
17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96Mhqdp020576
==============================End of - Headers==============================




From: david.mitchell-at-emu.usyd.edu.au
Date: Tue, 6 Oct 2009 18:24:23 -0500
Subject: [Microscopy] TEM Re: Calibration of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

Re: Calibration of TEM

There are very detailed ISO Standards for calibrating SEM and TEM
magnifications entitled:


Microbeam analysis - Scanning electron microscopy - Guidelines for
calibrating image magnification . ISO 16700.

http://www.iso.org/iso/catalogue_detail.htm?csnumber=30420



Microbeam analysis - Analytical transmission electron microscopy - Method
for calibrating image magnification by using reference materials giving a
periodic diffraction pattern. ISO/DIS 29301 (Draft).

http://www.iso.org/iso/catalogue_detail.htm?csnumber=45399



Regards,

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com


==============================Original Headers==============================
20, 27 -- From david.mitchell-at-usyd.edu.au Tue Oct 6 18:24:22 2009
20, 27 -- Received: from london.ucc.usyd.edu.au (london.ucc.usyd.edu.au [129.78.220.2])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96NOMjT004147
20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:24:22 -0500
20, 27 -- Received: from london.ucc.usyd.edu.au (localhost [127.0.0.1])
20, 27 -- by localhost (Postfix) with SMTP id 085872CACB8
20, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 10:24:20 +1100 (EST)
20, 27 -- Received: from ex-bri-pro-01.mcs.usyd.edu.au (ex-bri-pro-01.mcs.usyd.edu.au [172.17.63.241])
20, 27 -- by london.ucc.usyd.edu.au (Postfix) with ESMTP id EF68C2CAB40
20, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 10:24:19 +1100 (EST)
20, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by ex-bri-pro-01.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
20, 27 -- Wed, 7 Oct 2009 10:24:19 +1100
20, 27 -- Received: from 172.17.185.135 ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ;
20, 27 -- Tue, 6 Oct 2009 23:24:19 +0000
20, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
20, 27 -- Date: Wed, 07 Oct 2009 10:24:19 +1100
20, 27 -- Subject: TEM Re: Calibration of TEM
20, 27 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
20, 27 -- To: {Microscopy-at-microscopy.com}
20, 27 -- Message-ID: {C6F21CD3.1A6D%david.mitchell-at-emu.usyd.edu.au}
20, 27 -- Thread-Topic: TEM Re: Calibration of TEM
20, 27 -- Thread-Index: AcpG3CDEXw/rKrLPEd688gAjMrpsqg==
20, 27 -- Mime-version: 1.0
20, 27 -- Content-type: text/plain;
20, 27 -- charset="US-ASCII"
20, 27 -- Content-transfer-encoding: 7bit
20, 27 -- X-OriginalArrivalTime: 06 Oct 2009 23:24:19.0912 (UTC) FILETIME=[214FFC80:01CA46DC]
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Wed, 7 Oct 2009 09:19:41 -0500
Subject: [Microscopy] SEM: elemental sulfur

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree that a cold stage would be advisable. Vapor pressure depends on pressure, so the cold will definitely help.

However, I don't know how much an E-SEM will help. The issue is one of the partial pressure of S vapor, not just the pressure within the chamber. You could have 10 torr of pressure in the chamber, but the sulfur partial pressure is probably coming entirely from the elemental sulfur in the sample. Unless you can feed sulfur vapor as the environmental gas, the sulfur in the sample will sublime to bring up the sulfur pressure in the chamber. It will just be a question of time. It may be worse if the subliming gas is being quickly pumped away keeping the partial pressure of S close to zero. I suppose some sacrificial chunks of elemental sulfur off in a corner would help if they could be the source of sulfur vapor rather than the sulfur in your sample.

I was thinking of saying that the E-SEM or VP-SEM would be good in that the sulfur vapor is being swept away by the atmospheric gas of the sample chamber. However, if it is being effectively swept away, then that means your sample would continue to sublime and you still have your original problem. That takes you back to Scott's suggestion of a cold stage. It will serve to lower the equilibrium vapor pressure which will be better for your scope.

Since the question is one of rate, maybe you can observe crystals of pure elemental sulfur over time. You should be able to document the rate at which the size changes.

Warren

-----Original Message-----
X-from: swalck-at-southbaytech.com [mailto:swalck-at-southbaytech.com]
Sent: Tuesday, October 06, 2009 1:59 PM
To: wesaia-at-iastate.edu

You could freeze your sample by setting a low temperature on your ESEM Peltier stage. This is not the same as a cold stage obviously and I have no idea if it would be helpful - can anyone comment?

Dave

-----Original Message-----
X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net]
Sent: 06 October 2009 19:09
To: David Patton

As many of you are probably aware, elemental sulphur will evaporate within the SEM chamber. However we would still like to try using SEM-platformed image analysis to measure sulphur in metallurgical hydromet processing by-products.

Does anyone have a good idea of how fast sulphur will evaporate? That is, during the 1st hour it's difficult to see it going anywhere, but give it a day in the vacuum and it may be difficult to find. We'd like to think that less that 10% would disappear in the 1st hour - does anyone have more experience with it?

We'd also like to think that our ESEM would be designed such that evaporated sulphur would not pose problems for the electron column ... true?

TIA
~~~~~~~~~~~~~~~~~~~~~~~~~
cheerios, michael shaffer  :o)
SEM-MLA Research Coordinator
INCO Innovation Centre
Memorial University
St. John's Newfoundland
  http://www.mun.ca/creait/maf/






==============================Original Headers==============================
9, 28 -- From michael-at-shaffer.net Tue Oct 6 13:05:48 2009 9, 28 -- Received: from smtprelay.hostedemail.com (smtprelay0020.hostedemail.com [216.40.44.20])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n96I5mPT022559
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 13:05:48 -0500
9, 28 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254])
9, 28 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id C5047C3A275
9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:47 +0000 (UTC)
9, 28 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574
9, 28 -- X-Filterd-Recvd-Size: 2054
9, 28 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141])
9, 28 -- (Authenticated sender: michael-at-shaffer.net)
9, 28 -- by omf07.hostedemail.com (Postfix) with ESMTP
9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Oct 2009 18:05:45 +0000 (UTC)
9, 28 -- From: "michael shaffer" {michael-at-shaffer.net} 9, 28 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 9, 28 -- Subject: SEM: elemental sulfur 9, 28 -- Date: Tue, 6 Oct 2009 15:35:41 -0230 9, 28 -- Message-ID: {003901ca46af$a0e95db0$e2bc1910$-at-net}
9, 28 -- MIME-Version: 1.0
9, 28 -- Content-Type: text/plain;
9, 28 -- charset="iso-8859-1"
9, 28 -- X-Mailer: Microsoft Office Outlook 12.0 9, 28 -- Thread-Index: AcpGr5ocS33zrYBFRiGQPJNRKu0NFQ== 9, 28 -- Content-Language: en-ca 9, 28 -- x-cr-hashedpuzzle: PoE= AD4Z BRwz BTrI C9Uy Dgr/ DrG9 Dv21 FG4M F1L1 HXxw Is2A JV6w Kd/J LB48 LXHS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{4F43D163-E910-4167-88DC-848BCF8448E3};bQBpAGMAaABhAGUAbABAAHMAaABhAGYAZgBlAHIALgBuAGUAdAA=;Tue, 06 Oct 2009 18:05:35 GMT;UwBFAE0AOgAgAGUAbABlAG0AZQBuAHQAYQBsACAAcwB1AGwAZgB1AHIA
9, 28 -- x-cr-puzzleid: {4F43D163-E910-4167-88DC-848BCF8448E3}
9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n96I5mPT022559 ==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
21, 41 -- From David.Patton-at-uwe.ac.uk Wed Oct 7 09:19:41 2009
21, 41 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
21, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n97EJeel018065
21, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 09:19:40 -0500
21, 41 -- Received: from (unknown [164.11.132.62]) by mailapp04.uwe.ac.uk with smtp
21, 41 -- id 62ba_70758eec_b34c_11de_a62f_00142223915c;
21, 41 -- Wed, 07 Oct 2009 15:19:40 +0100
21, 41 -- Received: from egen-hub02.campus.ads.uwe.ac.uk
21, 41 -- (egen-hub02.uwe.ac.uk [164.11.251.46])
21, 41 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
21, 41 -- 2005)) with ESMTP id {0KR500G48EGN6X-at-mta02.uwe.ac.uk} for
21, 41 -- Microscopy-at-microscopy.com; Wed, 07 Oct 2009 15:19:35 +0100 (BST)
21, 41 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([169.254.1.146])
21, 41 -- by egen-hub02.campus.ads.uwe.ac.uk ([164.11.251.46]) with mapi; Wed,
21, 41 -- 07 Oct 2009 15:18:47 +0100
21, 41 -- Date: Wed, 07 Oct 2009 15:18:47 +0100
21, 41 -- From: David Patton {David.Patton-at-uwe.ac.uk}
21, 41 -- Subject: RE: [Microscopy] SEM: elemental sulfur
21, 41 -- In-reply-to: {200910061809.n96I9LS5000508-at-ns.microscopy.com}
21, 41 -- To: "'michael-at-shaffer.net'" {michael-at-shaffer.net}
21, 41 -- Message-id:
21, 41 -- {A169BAD2C2DC6D418270CDC03DF5CDF425542BA020-at-EGEN-MBX02.campus.ads.uwe.ac.uk}
21, 41 -- MIME-version: 1.0
21, 41 -- Content-type: text/plain;
21, 41 -- charset="utf-8"
21, 41 -- Content-language: en-US
21, 41 -- Accept-Language: en-US, en-GB
21, 41 -- Thread-topic: [Microscopy] SEM: elemental sulfur
21, 41 -- Thread-index: AcpGsCg1GY/BKipQT868KVmwEHPApwAqENuQ
21, 41 -- acceptlanguage: en-US, en-GB
21, 41 -- X-MS-Has-Attach:
21, 41 -- X-MS-TNEF-Correlator:
21, 41 -- References: {200910061809.n96I9LS5000508-at-ns.microscopy.com}
21, 41 -- X-NAIMIME-Disclaimer: 1
21, 41 -- X-NAIMIME-Modified: 1
21, 41 -- X-NAI-Spam-Level: **
21, 41 -- X-NAI-Spam-Score: 2
21, 41 -- X-NAI-Spam-Rules: 4 Rules triggered
21, 41 -- INVALID_MSGID=1, MFE_BAD_MSGID=0.5, RCVD_FROM_BY_SAME_IP_DIFF=0.5, RV3378=0
21, 41 -- Content-Transfer-Encoding: 8bit
21, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n97EJeel018065
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Wed, 7 Oct 2009 09:45:20 -0500
Subject: [Microscopy] SEM: elemental sulfur

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

My tables show the vapor pressure of sulfur (sulphur) as
1e-8 torr - 13C
1e-6 torr - 19C
1e-4 torr - 57C

I think that if you can keep the temperature down
(i.e. below 10C), you should be OK with looking
at sulfur containing materials. A peltier stage should be adequate.

A rough rule-of-thumb for deposition is that you
get 1 monolayer per second at a partial pressure
of 1e-6 torr. This can pretty easily be derived
using the ideal gas law and the kinetic energy of
a gas assuming a sticking coefficient of one.

While the loss of mass from the sample is indeed
a concern, I would be more concerned with the
redeposition on other surfaces within the
chamber, especially the polepiece of the
SEM. The e-beam may ionize the sulfur vapor
molecules causing them to stick more tenaciously
to the nearby surfaces. In this sense, I think
that trying to increase the sulfur partial
pressure by setting blocks of sulfur in the
chamber will, in the long run, be very
undesirable. The less sulfur in the chamber the
less contamination you will have.

Cheers,
Henk


At 10:20 AM 10/07/09, David.Patton-at-uwe.ac.uk wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events
from happening all at once. Lately it doesn't seem to be working.



==============================Original Headers==============================
14, 28 -- From colijn.1-at-osu.edu Wed Oct 7 09:45:20 2009
14, 28 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n97EjKfl001259
14, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 09:45:20 -0500
14, 28 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
14, 28 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
14, 28 -- id {01NELSX2HTF496Y79P-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
14, 28 -- Wed, 07 Oct 2009 10:45:18 -0400 (EDT)
14, 28 -- Received: from HOC1.ecr6.ohio-state.edu
14, 28 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
14, 28 -- (PMDF V6.3-x18 #31556)
14, 28 -- with ESMTPA id {01NELSX22GOC95S35X-at-ecr6.ohio-state.edu} ; Wed,
14, 28 -- 07 Oct 2009 10:45:18 -0400 (EDT)
14, 28 -- Date: Wed, 07 Oct 2009 10:47:40 -0400
14, 28 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
14, 28 -- Subject: Re: [Microscopy] RE: SEM: elemental sulfur
14, 28 -- In-reply-to: {200910071420.n97EKlts020178-at-ns.microscopy.com}
14, 28 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
14, 28 -- To: David.Patton-at-uwe.ac.uk
14, 28 -- Cc: Microscopy-at-microscopy.com
14, 28 -- Message-id: {01NELSX262NY95S35X-at-ecr6.ohio-state.edu}
14, 28 -- MIME-version: 1.0
14, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
14, 28 -- Content-type: text/plain; charset=iso-8859-1; format=flowed
14, 28 -- X-Env-From: auth/colijn.1-at-osu.edu
14, 28 -- References: {200910071420.n97EKlts020178-at-ns.microscopy.com}
14, 28 -- Content-Transfer-Encoding: 8bit
14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n97EjKfl001259
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Wed, 7 Oct 2009 10:41:04 -0500
Subject: [Microscopy] Re: viaWWW: TEM: Clogged microscope plumbing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have posted the chemistry of white calcium carbonate and green copper
carbonate scaling before to this list. I even wrote an unpublished article
on this chemistry. It contained the derived Ksp value for copper carbonate
(~5 x 10^-14) which is less soluble than calcium carbonate. The chemistry
is more complicated than just looking at each ion separately. There is a
common ion effect (CO3^-2) and equilibrium to consider.

Pumping acid through microscope tubing can cause harm if done improperly.
To pump any acid mixture through a chiller that is not plugged up is not
what I would do. My servicemen and I always setup a separate small pump
and bucket to circulate the acid only through the microscope. One thing
that happens is that pieces of black rubber tubing flake off, loosened
carbonate scale flakes off, and bubbles are seen going to the bucket.
Circulating that stuff to a chiller and etching those larger diameter pipes
is not what FEI and I did.

You should test the water for the actual cause of any green color in the
water. Add HCl to dissolve all scale and then add an excess of ammonium
hydroxide to your sample. If it turns deep blue, then you probably
exceeded the solubility product for the copper scale. If you have copper
ions in solution, then algae will not grow. This is a simple test to
determine what is going on but we also used atomic absorption to follow the
copper chemistry. No blue color means algae. To prevent scaling from an
open chiller reservoir, you must lower the copper ion concentration by
draining the chiller water, say 33% of it, every three months. This
requires an automatic DI water refill system when the chiller is in a
remote location. This action lowers the ion concentrations so that the Ksp
is not exceeded with either calcium or copper carbonate.

Installing all plastic pipes and using a HVAC heat exchanger system to
eliminate a chiller copper lines will not stop the scaling from copper
carbonate. The pump heads and the microscope lines have copper alloys in
them like bronze impellers. You can never eliminate all copper sources and
we tried. What is the source of calcium and magnesium? Incomplete
flushing of the city water "dead volume" after any chiller disruption where
you (or someone else on your common chilled water supply) switched
temporarily to city water. What is the carbonate source? The stirred
reservoir that is open to air entrainment. Just look at a green copper
roof on a church. It first turns brown from copper oxide but this layer is
not a self protective layer like aluminum oxide. With more weathering, the
"tarnished" copper roof turns green from exposure to rain water and air.
Your chiller supplies the water and the open stirred reservoir supplies the
dissolved air.

JMO,

Paul Beauregard

At 07:17 AM 10/6/09 -0500, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
8, 20 -- From beaurega-at-westol.com Wed Oct 7 10:41:04 2009
8, 20 -- Received: from smtpauth.winbeam.com (smtpauth.winbeam.com [64.84.96.53])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n97Ff3Qm018967
8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 10:41:04 -0500
8, 20 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
8, 20 -- by smtpauth.winbeam.com (8.13.1/8.13.1) with SMTP id n97Ff2Wb017996
8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 11:41:02 -0400
8, 20 -- Received: (qmail 19965 invoked by uid 89); 7 Oct 2009 15:40:58 -0000
8, 20 -- Received: from pitts-69-72-118-36.dynamic-dialup.coretel.net (HELO running) (69.72.118.36)
8, 20 -- by mail.winbeam.com with SMTP; 7 Oct 2009 15:40:58 -0000
8, 20 -- Message-Id: {3.0.6.32.20091007114121.007e1cc0-at-pop3.norton.antivirus}
8, 20 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified)
8, 20 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
8, 20 -- Date: Wed, 07 Oct 2009 11:41:21 -0400
8, 20 -- To: microscopy-at-microscopy.com
8, 20 -- From: Beaurega {beaurega-at-westol.com}
8, 20 -- Subject: Re: [Microscopy] viaWWW: TEM: Clogged microscope plumbing
8, 20 -- In-Reply-To: {200910061217.n96CHmpc018281-at-ns.microscopy.com}
8, 20 -- Mime-Version: 1.0
8, 20 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: jkrupp-at-deltacollege.edu
Date: Wed, 7 Oct 2009 11:48:40 -0500
Subject: [Microscopy] Delta College Students/Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I am teaching courses in EM techniques in the technology program at
Delta College in Stockton.

Our students are looking forward to working in the EM field, and to
introduce them to the community and perhaps their future colleagues, I
have asked them all to subscribe to the Listserver. So far, I think
about 15 of them have done this.

This is just to let you know that these students are dedicated to EM,
but sometimes lack the background to formulate excellent questions. I
have asked them to clear anything they want to post with me, but some
will no doubt leak through. If you see a question or response from a
Delta student, be kind and remember they are new to this game. If it
gets out of hand, let me know and I will take care of resolving any
problems.

The students have also asked for mock interviews so they can know what
to expect in their job hunting. We are located in N. California, about
60 miles south of Sacramento. If anyone would like visit Delta for a
day or so to do some trial runs, let me know and I will arrange it.
Best I can offer is lunch and maybe a chance to see some young and
enthusiastic EM students.

I remain, your humble servant,

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
11, 37 -- From jkrupp-at-deltacollege.edu Wed Oct 7 11:48:40 2009
11, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
11, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n97GmdFG005171
11, 37 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 11:48:40 -0500
11, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
11, 37 -- by localhost (Email Security Appliance) with SMTP id 04C961B9431_ACCC667B
11, 37 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 16:48:39 +0000 (GMT)
11, 37 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236])
11, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id EB556192CA7_ACCC666F
11, 37 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 16:48:38 +0000 (GMT)
11, 37 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
11, 37 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
11, 37 -- with ESMTP id 49739851 for microscopy-at-microscopy.com; Wed, 07 Oct 2009 09:48:38 -0700
11, 37 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by
11, 37 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
11, 37 -- ESMTP id KR5LD200.RHE for {microscopy-at-microscopy.com} ; Wed, 7
11, 37 -- Oct 2009 09:48:38 -0700
11, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1])
11, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 7FFB87A38639
11, 37 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 09:48:38 -0700 (PDT)
11, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu
11, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
11, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
11, 37 -- with ESMTP id j8kaig6Fz5Nw for {microscopy-at-microscopy.com} ;
11, 37 -- Wed, 7 Oct 2009 09:48:38 -0700 (PDT)
11, 37 -- Received: from [172.20.3.214] (unknown [172.20.3.214])
11, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 543917A3862D
11, 37 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 09:48:38 -0700 (PDT)
11, 37 -- Message-Id: {F3A52B2D-53B8-4FBF-B775-3DF2FCBE4C0C-at-deltacollege.edu}
11, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
11, 37 -- To: microscopy-at-microscopy.com
11, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 37 -- Content-Transfer-Encoding: 7bit
11, 37 -- Mime-Version: 1.0 (Apple Message framework v936)
11, 37 -- Subject: Delta College Students/Questions
11, 37 -- Date: Wed, 7 Oct 2009 09:48:38 -0700
11, 37 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: schooley-at-mcn.org
Date: Wed, 7 Oct 2009 15:08:27 -0500
Subject: [Microscopy] Microscopy education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There was a listserver discussion recently about the use of SEMs in
high schools. As part of that, I asked for a volunteer to organize a
discussion group for high schools that have a scanning electron
microscope in their school. Margo Gill-Linscott {
analytic-at-rawbw.com} , a recently-retired scanning microscopist, has
volunteered to take on this task and will put together a database of
high schools with SEMs. We know of possibly 7 in the U.S: in Cold
Spring, NY, Philadelphia, Waynesburg, & Red Lion, PA, Teterboro, NJ,
Vancouver, WA, & Beaverton, OR; please reply directly to her with
information about them, or others that we've missed.

Margo has already set up an Email group to provide an avenue for
discussion. The address is: hs_SEM-at-googlegroups.com. Please join
the group, add comments, a history of your experience if you have a
SEM in your school, frustrations you've encounterd, lesson plans,
etc.; anything that could provide insight into the use of the tool
and information which might be helpful in attracting others who might
be interested in helping other high schools. A SEM is without a
doubt one of the greatest learning tools available for encouraging
science interest amongst young people.

--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
45301 Caspar Point Road, Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
3, 16 -- From schooley-at-mcn.org Wed Oct 7 15:08:26 2009
3, 16 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85])
3, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n97K8OEN031886
3, 16 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 15:08:26 -0500
3, 16 -- Received: from [66.81.36.165] (port=51697)
3, 16 -- by smtp1.mcn.org with esmtpa (Exim 4.63)
3, 16 -- (envelope-from {schooley-at-mcn.org} )
3, 16 -- id 1MvcoB-0001Bf-3F
3, 16 -- for microscopy-at-microscopy.com; Wed, 07 Oct 2009 13:08:24 -0700
3, 16 -- Mime-Version: 1.0
3, 16 -- Message-Id: {a06200700c6f2a3016a0d-at-[66.81.212.43]}
3, 16 -- Date: Wed, 7 Oct 2009 12:59:02 -0700
3, 16 -- To: microscopy-at-microscopy.com
3, 16 -- From: Caroline Schooley {schooley-at-mcn.org}
3, 16 -- Subject: Microscopy education
3, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: JEEvans-at-ucdavis.edu
Date: Wed, 7 Oct 2009 16:48:08 -0500
Subject: [Microscopy] viaWWW: POSTDOCTORAL POSITION IN DYNAMIC TRANSMISSION ELECTRON

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both JEEvans-at-ucdavis.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: JEEvans-at-ucdavis.edu
Name: James Evans

Organization: University of California, Davis

Title-Subject: [Filtered] Postdoctoral Research Position

Question: POSTDOCTORAL POSITION IN DYNAMIC TRANSMISSION ELECTRON MICROSCOPY

MOLECULAR AND CELLULAR BIOLOGY/CHEMICAL ENGINEERING AND MATERIALS
SCIENCE DEPARTMENT, UNIVERSITY OF CALIFORNIA-DAVIS, DAVIS CA 95616. USA

A postdoctoral position is currently available to work on an NIH
funded project to develop the dynamic TEM to study live biological
processes. This DTEM project at UC-Davis aims to couple high time
resolution (microseconds to nanoseconds) to high spatial resolution
by using a pulsed photoemission source for the microscope. This
development has already seen advances in in-situ studies of phase
transformations and the current postdoctoral position aims to expand
the area of research for the DTEM into the biological
sciences. Successful candidates will be recent Ph.D. graduates in
physics, chemistry, biology, or materials science with a sound
background in the relevant instrumentation issues and an ambition to
be part of a developing program pushing at the frontiers of
structural biology. Prior experience in high resolution TEM/STEM,
cryo-EM or in-situ TEM is essential. However, consideration will be
based on the candidates overall potential for success in the field
and applicants with prior experience in related fields are encouraged
to apply. Salary is commensurate with experience and is determined
within the UC-Davis standard pay scales for postdoctoral
appointees. Further information on the postdoctoral positions can be
obtained by contacting Professor Nigel D. Browning
(nbrowning-at-ucdavis.edu) or Dr. James Evans (jeevans-at-ucdavis.edu).

****************************************************************************

Login Host: 76.246.59.233
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Wed Oct 7 16:48:08 2009
9, 12 -- Received: from [129.6.248.221] ([130.202.238.72])
9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n97Lm6A7016752
9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2009 16:48:07 -0500
9, 12 -- Mime-Version: 1.0
9, 12 -- Message-Id: {p06240803c6f2bd010371-at-[129.6.248.221]}
9, 12 -- Date: Wed, 7 Oct 2009 17:48:04 -0400
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: JEEvans-at-ucdavis.edu (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: POSTDOCTORAL POSITION IN DYNAMIC TRANSMISSION ELECTRON
9, 12 -- MICROSCOPY
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ariya001-at-umn.edu
Date: Thu, 8 Oct 2009 07:57:30 -0500
Subject: [Microscopy] Re: Switching specimen from paraffin to resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Nilcat Yelmaz,

Deparaffinize the blocks (melt the blocks, followed by in xylene for 3 hours
at 50 degree Celsius)

Wash for 30 minutes in 100% ethanol.

Post-fix in 0.25% Osmium tetroxide in 97.5% ethanol for 1 hour.

Three rinses in 100% ethanol followed by infiltration in resin and embed the
samples.

I am not sure about the original reference; probably someone in the server
can help you with that.

Good luck

Don Ariyakumar
Assistant Scientist
Veterinary Diagnostic Laboratory
University of Minnesota
http://www.vdl.umn.edu/ourservices/ElectronMicroscopy/home.html

(Confidentiality Notice: This message, together with any attachments, is
intended only for the use of the individual or entity to which it is
addressed and may contain confidential or privileged information. If you
think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.)


==============================Original Headers==============================
10, 24 -- From ariya001-at-umn.edu Thu Oct 8 07:57:30 2009
10, 24 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n98CvU2T008699
10, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 07:57:30 -0500
10, 24 -- Received: from VDL00316AD (x-134-84-29-22.ansci.umn.edu [134.84.29.22])
10, 24 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
10, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 07:57:30 -0500 (CDT)
10, 24 -- X-Umn-Remote-Mta: [N] x-134-84-29-22.ansci.umn.edu [134.84.29.22] #+LO+TS+AU+HN
10, 24 -- X-Umn-Classification: local
10, 24 -- Message-ID: {B1C23AF4CA92403BAF0DFA2B51C7D52C-at-auxs.umn.edu}
10, 24 -- From: "Don Ariyakumar" {ariya001-at-umn.edu}
10, 24 -- To: {Microscopy-at-microscopy.com}
10, 24 -- Subject: Re: Switching specimen from paraffin to resin
10, 24 -- Date: Thu, 8 Oct 2009 07:57:29 -0500
10, 24 -- MIME-Version: 1.0
10, 24 -- Content-Type: text/plain;
10, 24 -- format=flowed;
10, 24 -- charset="iso-8859-1";
10, 24 -- reply-type=original
10, 24 -- Content-Transfer-Encoding: 7bit
10, 24 -- X-Priority: 3
10, 24 -- X-MSMail-Priority: Normal
10, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843
10, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: SanfordEJ-at-Corning.com
Date: Thu, 8 Oct 2009 13:15:26 -0500
Subject: [Microscopy] FW: LM - Help in finding vendors of Confocal Microscope to look at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am currently investigating the potential purchase of a confocal
microscope system to look at material samples and want to make sure I am
evaluating all of the viable suppliers. What I think I am looking for is
a system that has multiple laser lines (near-UV to near-IR), makes
topography measurements and has the ability to capture fluorescence
images. The ability to capture confocal Raman images would be a plus.

What I would like from to obtain from the members of this group is a
list of confocal suppliers that might be able to provide the system I
have described above. Inclusion of strengths and weaknesses of the
systems, if known, would also be appreciated.


Thank you for your help,
Earl Sanford
sanfordej-at-corning.com


==============================Original Headers==============================
5, 33 -- From SanfordEJ-at-Corning.com Thu Oct 8 13:15:26 2009
5, 33 -- Received: from exprod6og115.obsmtp.com (exprod6og115.obsmtp.com [64.18.1.35])
5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n98IFOeh005516
5, 33 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Oct 2009 13:15:25 -0500
5, 33 -- Received: from source ([199.197.135.113]) (using TLSv1) by exprod6ob115.postini.com ([64.18.5.12]) with SMTP
5, 33 -- ID DSNKSs4sPJzJmvHxEzfgwcX03pP/uT4ZLjQr-at-postini.com; Thu, 08 Oct 2009 11:15:25 PDT
5, 33 -- Received: from CVCV0XI13.na.corning.com
5, 33 -- (cvcv0xi13.na.corning.com [10.180.42.13])
5, 33 -- by npostal.corning.com (PMDF V6.3-2x2 #31555)
5, 33 -- with SMTP id {0KR7008M4K1NJH-at-npostal.corning.com} for
5, 33 -- Microscopy-at-Microscopy.com; Thu, 08 Oct 2009 14:15:23 -0400 (EDT)
5, 33 -- Received: from cvcv0xi04.na.corning.com ([10.180.42.31])
5, 33 -- by CVCV0XI13.na.corning.com with Microsoft SMTPSVC(6.0.3790.3959); Thu,
5, 33 -- 08 Oct 2009 14:14:50 -0400
5, 33 -- Date: Thu, 08 Oct 2009 14:14:07 -0400
5, 33 -- From: "Sanford, Earl J" {SanfordEJ-at-Corning.com}
5, 33 -- Subject: FW: LM - Help in finding vendors of Confocal Microscope to look at
5, 33 -- Material Samples
5, 33 -- To: Microscopy-at-Microscopy.com
5, 33 -- Message-id: {7C6B23A27CA15241AFBF747F53B49F2C01AC7503-at-cvcv0xi04.na.corning.com}
5, 33 -- MIME-version: 1.0
5, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
5, 33 -- Content-type: text/plain; charset=us-ascii
5, 33 -- Thread-Topic: LM - Help in finding vendors of Confocal Microscope to look at
5, 33 -- Material Samples
5, 33 -- thread-index: AcpIQlA8I1PYidFuQtewfTJWTdgKiwAAF80A
5, 33 -- Content-class: urn:content-classes:message
5, 33 -- X-MS-Has-Attach:
5, 33 -- X-MS-TNEF-Correlator:
5, 33 -- X-OriginalArrivalTime: 08 Oct 2009 18:14:50.0251 (UTC)
5, 33 -- FILETIME=[39C215B0:01CA4843]
5, 33 -- Content-Transfer-Encoding: 8bit
5, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n98IFOeh005516
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Thu, 8 Oct 2009 15:51:33 -0500
Subject: [Microscopy] LEICA S360FE SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,
I recently had the pleasure of moving a S360FE. Actually, it went
very well, everything seemed to go very smoothly. The SEM boots
normally and the vacuum is very good, 1 x 10-9 in the Gun, mid 7's in
the Chamber. However, when I go to run up the FE Gun, my extraction
current stops at about 12ua(needs to be between 150 - 175 to develop the
beam). The SEM imaged normally before the move..... Any help out
there? Reply offline if you wish. Thanks in advance

Gary Easton
Scanners Corporation
3rd Party SEM Service
410-857-7633
--


==============================Original Headers==============================
3, 19 -- From garyeaston-at-scannerscorp.com Thu Oct 8 15:51:33 2009
3, 19 -- Received: from omr2.networksolutionsemail.com (omr2.networksolutionsemail.com [205.178.146.52])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n98KpXKr028056
3, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 15:51:33 -0500
3, 19 -- Received: from mail.networksolutionsemail.com (ns-omr2.mgt.netsol.com [10.49.6.65])
3, 19 -- by omr2.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n98KpWLe020597
3, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 16:51:32 -0400
3, 19 -- Received: (qmail 28449 invoked by uid 78); 8 Oct 2009 20:51:32 -0000
3, 19 -- Received: from unknown (HELO ?192.168.1.3?) (72.81.181.240)
3, 19 -- by ns-omr2.lb.hosting.dc2.netsol.com with SMTP; 8 Oct 2009 20:51:32 -0000
3, 19 -- Message-ID: {4ACE509D.7010502-at-scannerscorp.com}
3, 19 -- Date: Thu, 08 Oct 2009 16:50:37 -0400
3, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 19 -- MIME-Version: 1.0
3, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 19 -- Subject: LEICA S360FE SEM
3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nicholsj-at-musc.edu
Date: Thu, 8 Oct 2009 19:34:08 -0500
Subject: [Microscopy] viaWWW: Name for simultaneous reflectance/fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nicholsj-at-musc.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nicholsj-at-musc.edu
Name: James Nicholson

Organization: Coral Culture & Collaborative Research Facility, NOAA
NOS NCCOS, CCEHBR, Fort Johnson Marine Lab, Charleston, SC

Title-Subject: [Filtered] Name for simultaneous
reflectance/fluorescence microscopy with monochrome illumination

Question: In doing research on coral auto fluorescence we have
developed a technique which we have named Monochrome
Reflectance/Fluorescence Illumination (essentially it is
epifluorescence without a barrier filter). We use a 480nm 5W LED (or
a similar 430nm), lamp but removing the barrier filter from a
standard FITC cube would work much the same.
Normally a barrier filter is used in fluorescence microscopy to block
the reflected excitation illumination which would generally drown out
the weaker fluorescent signal. Certain coral, especially Fungia, can
have such a strong fluorescence that it can be seen over the
reflected light. Where the tissue is thicker the green fluorescent
protein (GFP) fluoresces so brightly that the tissue appears green.
Over the septa, the tissue is thinner and the blue excitation light
reflects off of the white underlying skeleton. We have found this to
be a very effective way to follow changes in thickness and patterns
of thickness of the tissue. Thinning is often an early indicator of
disease and eventual death.
In my 34 years of microscopy, I have not seen any reference to this
technique or a name for it. Before I publish, does anyone know of an
existing name for this technique or can suggest one?


Login Host: 128.23.3.146
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Thu Oct 8 19:34:08 2009
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n990Y7f1020711
7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 19:34:08 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- Message-Id: {p06240802c6f4355eae61-at-[206.69.208.22]}
7, 12 -- Date: Thu, 8 Oct 2009 19:34:06 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: nicholsj-at-musc.edu (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Name for simultaneous reflectance/fluorescence microscopy
7, 12 -- with monochrome illumination
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zerfasp-at-ors.od.nih.gov
Date: Thu, 8 Oct 2009 19:34:37 -0500
Subject: [Microscopy] viaWWW: CSM Fall dinner meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zerfasp-at-ors.od.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: zerfasp-at-ors.od.nih.gov
Name: Patricia Zerfas

Title-Subject: [Filtered] CSM Fall dinner meeting

Question: Does anyone know if The Chesapeake Society for Microscopy
is planning a fall dinner meeting?

Login Host: 128.231.171.150
---------------------------------------------------------------------------

==============================Original Headers==============================
5, 11 -- From zaluzec-at-microscopy.com Thu Oct 8 19:34:36 2009
5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n990YY0N021226
5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Oct 2009 19:34:36 -0500
5, 11 -- Mime-Version: 1.0
5, 11 -- Message-Id: {p06240803c6f4358ab8ae-at-[206.69.208.22]}
5, 11 -- Date: Thu, 8 Oct 2009 19:34:33 -0500
5, 11 -- To: microscopy-at-microscopy.com
5, 11 -- From: zerfasp-at-ors.od.nih.gov (by way of MicroscopyListserver)
5, 11 -- Subject: viaWWW: CSM Fall dinner meeting
5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Pamela.Lloyd-at-WPAFB.AF.MIL
Date: Fri, 9 Oct 2009 05:30:35 -0500
Subject: [Microscopy] MSORV Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI,

For those of you in the Ohio River Valley Area, the Microscopy Society
of the Ohio River Valley (MSORV) will be hosting their fall meeting on
Wednesday, October 14, 2009 at Battelle Memorial Institute in Columbus,
OH from 3-7PM. There is no registration fee to attend, but Battelle's
security will require your name, affiliation, and country of
citizenship, ahead of time in order to get in. So, after reviewing the
agenda, if you would like to attend, please send an e-mail to me, Pamela
Lloyd (MSORV Secretary). Additional information, directions, parking
info, can be found on line at the MSORV website, www.msorv.org.

Fall MSORV Meeting
October 14, 2009
AGENDA

3:00-3:50 PM Opening Events:
Registration and Mixer (Sponsored by Proctor &
Gamble)
Visit Vendor's tables

3:50-4:00 PM Welcome: by Ed Calomeni, MSORV President and Dan
Kremser, Battelle Memorial Institute.

4:00-5:00 PM Keynote Speaker - Kim Rensing, Leica Microsystems.
"New Advances in Cryological Sample
Preparation for Electron Microscopy".

5:00-5:20PM Business Meeting (led by Ed Calomeni, MSORV
President)
Minutes of Spring Meeting: Located
on-line: www.msorv.org
Treasurer's Report: Judy
Mescher, Treasurer
Election Results:
Pam Lloyd, Secretary
Old Business:
New Business: Possible
Spring 2010 Meeting Site(s)
Possible
speakers for Spring 2010

10 min break Continue Mixer/Visit Vendors

5:30-6:50PM MAS Tour Speaker - Paul Hlava, Retired, Sandia
National Lab,
and owner of Access to Gems & Minerals, Inc. The
talk is
"Gemstone Synthesis".

Final Words: Ed Calomeni, MSORV President


==============================Original Headers==============================
11, 30 -- From Pamela.Lloyd-at-WPAFB.AF.MIL Fri Oct 9 05:30:35 2009
11, 30 -- Received: from fohfwb004.oh.afmc.af.mil (fohfwb004.oh.afmc.af.mil [131.28.29.208])
11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99AUYNH015101
11, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 05:30:34 -0500
11, 30 -- Received: from FOHMLRL02.enterprise.afmc.ds.af.mil (fohmlrl02.enterprise.afmc.ds.af.mil [131.28.34.156])
11, 30 -- by fohfwb004.oh.afmc.af.mil with ESMTP id n99AUYs7028197
11, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 10:30:34 GMT
11, 30 -- X-AuditID: 831c229c-00001ed0000006e0-72-4acf10ca44ff
11, 30 -- Received: from FOHMLBH04.Enterprise.afmc.ds.af.mil ([10.28.34.8]) by FOHMLRL02.enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.3959);
11, 30 -- Fri, 9 Oct 2009 06:30:34 -0400
11, 30 -- Received: from VFOHMLAO13.Enterprise.afmc.ds.af.mil ([131.28.34.133]) by FOHMLBH04.Enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.3959);
11, 30 -- Fri, 9 Oct 2009 06:30:33 -0400
11, 30 -- Content-class: urn:content-classes:message
11, 30 -- MIME-Version: 1.0
11, 30 -- Content-Type: text/plain;
11, 30 -- charset="us-ascii"
11, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
11, 30 -- Subject: MSORV Fall Meeting
11, 30 -- Date: Fri, 9 Oct 2009 06:29:59 -0400
11, 30 -- Message-ID: {29A7C5F65D54744CBFD97CD62A33DEE905A39F03-at-VFOHMLAO13.Enterprise.afmc.ds.af.mil}
11, 30 -- X-MS-Has-Attach:
11, 30 -- X-MS-TNEF-Correlator:
11, 30 -- Thread-Topic: MSORV Fall Meeting
11, 30 -- Thread-Index: AcpIy3QWftf7b45SS/uNU/FakyFTiQ==
11, 30 -- From: "Lloyd, Pamela F CTR USAF AFMC AFRL/RXPJ" {Pamela.Lloyd-at-WPAFB.AF.MIL}
11, 30 -- To: {Microscopy-at-microscopy.com}
11, 30 -- X-OriginalArrivalTime: 09 Oct 2009 10:30:33.0692 (UTC) FILETIME=[885E41C0:01CA48CB]
11, 30 -- X-Brightmail-Tracker: AAAAAA==
11, 30 -- Content-Transfer-Encoding: 8bit
11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n99AUYNH015101
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Fri, 9 Oct 2009 07:48:34 -0500
Subject: [Microscopy] light objective search

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an automated hardness test with uses a computer system to optically
measure the diamond-shaped depressions that determine hardness. We
currently have a 20x and 5x no name metallographic objectives and would
like to replace these with other objectives. At lighter testing loads and
hard samples the indents are smaller and require higher magnification to
measure.

Our concern is they should be DIM or RMS standard mount, have a body length
of around 45mm (so they don't strike the sample mounting stage when the
sample is being indented) and be suitable for coaxial illumination. While
we would like to just add a couple objectives comparable with our two
current ones, we would not be adverse to purchasing a set of four, namely
5, 10, 20 and 40x.

Dealers are welcome as is any advice or experience in how other people have
resolved this problem.

thanks.......
Frank Karl...
Lincoln Electric Company
Cleveland Ohio
216-383-2090

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
7, 21 -- From frank_karl-at-lincolnelectric.com Fri Oct 9 07:48:34 2009
7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99CmXNb004488
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 07:48:33 -0500
7, 21 -- Subject: light objective search
7, 21 -- To: Microscopy-at-microscopy.com
7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
7, 21 -- Message-ID: {OF84227F2D.6AB757B8-ON8525764A.0041EDF9-8525764A.00464FD7-at-lincolnelectric.com}
7, 21 -- Date: Fri, 9 Oct 2009 08:48:36 -0400
7, 21 -- From: Frank_Karl-at-lincolnelectric.com
7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 10/09/2009 08:48:07 AM,
7, 21 -- CD-MIME complete at 10/09/2009 08:48:07 AM,
7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 10/09/2009 08:48:07 AM,
7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
7, 21 -- 07, 2008) at 10/09/2009 08:48:07 AM,
7, 21 -- Serialize complete at 10/09/2009 08:48:07 AM
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Fri, 9 Oct 2009 08:02:30 -0500
Subject: [Microscopy] Target base for hummer 1

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have any extra bases for targets for the Hummer 1 sputter coater
(technics)? Alternatively, do you know where I can purchase new bases?

We can have new targets inserted but really need the mounting for them.

Thanks,
Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy/



==============================Original Headers==============================
5, 31 -- From dsherman-at-purdue.edu Fri Oct 9 08:02:30 2009
5, 31 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99D2UY3019228
5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 08:02:30 -0500
5, 31 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127])
5, 31 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n99D2TlV010720
5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:02:29 -0400
5, 31 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91])
5, 31 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n99D2Tif013771
5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:02:29 -0400
5, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.16]) by WPPEXHUB02F.purdue.lcl
5, 31 -- ([::1]) with mapi; Fri, 9 Oct 2009 09:02:29 -0400
5, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
5, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
5, 31 -- Date: Fri, 9 Oct 2009 09:02:27 -0400
5, 31 -- Subject: Target base for hummer 1
5, 31 -- Thread-Topic: Target base for hummer 1
5, 31 -- Thread-Index: AcpI4MBSjL7wwYB/TMu/B7mGXtQ0ZA==
5, 31 -- Message-ID: {C6F4ACA3.13C1%dsherman-at-purdue.edu}
5, 31 -- Accept-Language: en-US
5, 31 -- Content-Language: en-US
5, 31 -- X-MS-Has-Attach:
5, 31 -- X-MS-TNEF-Correlator:
5, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
5, 31 -- acceptlanguage: en-US
5, 31 -- Content-Type: text/plain; charset="us-ascii"
5, 31 -- MIME-Version: 1.0
5, 31 -- X-PMX-Version: 5.5.7.378829
5, 31 -- X-PerlMx-Virus-Scanned: Yes
5, 31 -- Content-Transfer-Encoding: 8bit
5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n99D2UY3019228
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Fri, 9 Oct 2009 08:36:46 -0500
Subject: [Microscopy] Cleaning aluminum parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unfortunately some aluminum planchettes used in high pressure freezing were
left in water containing detergent for a few hours before cleaning was
completed. As a result the planchettes turned dark in color.

It is much more difficult to work with them in this condition. Does anyone
know a way to remove the tarnish and make them nice and shiny again?

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy





==============================Original Headers==============================
9, 31 -- From dsherman-at-purdue.edu Fri Oct 9 08:36:45 2009
9, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99DajH5002451
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 08:36:45 -0500
9, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
9, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n99DajKA007535
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45 -0400
9, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92])
9, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n99DajKp012208
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45 -0400
9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.16]) by WPPEXHUB03H.purdue.lcl
9, 31 -- ([172.21.6.92]) with mapi; Fri, 9 Oct 2009 09:36:44 -0400
9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
9, 31 -- Date: Fri, 9 Oct 2009 09:36:41 -0400
9, 31 -- Subject: Cleaning aluminum parts
9, 31 -- Thread-Topic: Cleaning aluminum parts
9, 31 -- Thread-Index: AcpI5YiahaNevBxLQ1qYopsj7xAsxA==
9, 31 -- Message-ID: {C6F4B4A9.13DB%dsherman-at-purdue.edu}
9, 31 -- Accept-Language: en-US
9, 31 -- Content-Language: en-US
9, 31 -- X-MS-Has-Attach:
9, 31 -- X-MS-TNEF-Correlator:
9, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
9, 31 -- acceptlanguage: en-US
9, 31 -- Content-Type: text/plain; charset="us-ascii"
9, 31 -- MIME-Version: 1.0
9, 31 -- X-PMX-Version: 5.5.7.378829
9, 31 -- X-PerlMx-Virus-Scanned: Yes
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n99DajH5002451
==============================End of - Headers==============================




From: amenex-at-spamcop.net
Date: Fri, 9 Oct 2009 11:34:02 -0500
Subject: [Microscopy] Setting up legacy digital microscope cameras on more recent

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In a similar vein to the recent discussion about pixel count and
resolution ...

I've been attempting to set up a series of legacy digital cameras on
metallurgical microscopes recently. Recent results can be seen
here:
http://www.georgesbasement.com/NomarskiDIC/ProgressThru09302009/Research-I-Setup.htm

Various problems arise for the following digital microscope cameras:

Leaf Systems DCB-II: What monitors are compatible with the Mac
Power Mac 7100/80 ? I've managed to get one Philips monitor to work
after a fashion and a very old CrystalScan 1024 to work at 640 by 480
pixel resolution, but I'd like to know what works "out of the box," that
is, straight off eBay, as no one is selling anything on the retail market.

The 31mm square sensor has 2048 by 2048 pixels - just right for nice
photomicrographs, but the files are huge: 25MB for a 48-bit per pixel
TIFF image. 14 bits of useful dynamic range. That's the great thing
about this camera. Gets detail from the highlights of the polished
(unetched) metal surface of a cast iron as well as details in the graphite
flakes. Focuses just like a view camera - the clear polycarbonate screen
I made is over two inches across, and a Reichert 6X focusing loupe will
allow one to view everything on the screen, a little at a time, with no
"ground glass" to interfere with critical focus. I find that a B&L 7.0X
loupe works best. Focus is super-critical at such high image resolution.

Sound Vision, Inc. CMOS-Pro: Works fine on a W98SE system, but I'd
like to get a WinXP laptop working with it, but the camera isn't
recognized by the TWAIN plugin for Adobe Photoshop. Has anyone
ever overcome this problem ?

This camera sports only a 1000 by 800 pixel sensor, but it captures
three (red, green, blue) ten-bit per pixel images. Also very nice; one-
tenth the original price of the DCB-II. Interfacing to the Olympus
Stereo is easy with the C-mount female threads on the camera, and the
adapter I made for the Kodak MDS-100 works fine holding the CMOS-
Pro on the Bausch & Lomb Research-I. Versatile. Focus is live and on
the PC screen.

Leaf Systems Microlumina: Everything's present except the infrared
filter and the EasyScan software. I found a very old post with a similar
request on this listserv ... This is a scanning camera with more than
enough total pixels if one can ever get it together ... lighting has to be
super-steady, as AC light sources create imaging havoc.

MegaVision MegaVision T2: A basket case. I need the PCI card, some
cables, and the software. Competitor to the DCB-II and in between
that and the CMOS-Pro in pixel count. Also a three-shot camera. The
camera's in fine shape, but someone left the card and cables with the
original PC while carefully extracting the card's specialized power
supply, which had been fitted into a case to fit in a hard drive slot
and connect to the PC's Molex plug.

Olympus E-Volt E-500: Sensor is the size of 35mm movie film images,
and the pixel count can be set up to 3264 by 2468 in the recorded
image. It's billed as an eight megapixel camera. I was able to interface
it to the Olympus SZH stereo camera using the adapter meant for their
former 35mm SLR. I also made an adapter for the Bausch & Lomb
Research-I metallograph. Focus is difficult to achieve, even with 2.5X
magnification of the tiny through-the-lens viewer. A live feed to the
PC would be a handy improvement.

Sony Mavica FD-92: With a screw-in microscope adapter (just a
dedicated eyepiece) it's the easiest of all these cameras to use, as it
will autofocus the microscope image extremely reliably without using
a dedicated shutter release, as the effective film speed is so very fast.
Automatic exposure, too. A point-and-click microscope camera ! The
Memory Stick flash memory hold more images than you have time to
shoot. Pixel count is 1024 by 768, plenty big enough for report images.
The zoom feature allows the microscope image to almost fill the image
sensor, but the corners might sometimes get cut off.

Kodak MDS-100: Works OK on a W98SE system; only 640 by 480 native
resolution. This is the least number of useful pixels in my opinion.

Kodak DC-40: This can be set up with a microscope adapter and
produces 640 by 480 images. Uses a built-in flash memory, and can
be read out with the W98SE software that Kodak provided.

George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
amenex-at-amenex.com
http://www.amenex.com/


==============================Original Headers==============================
15, 26 -- From amenex-at-spamcop.net Fri Oct 9 11:34:02 2009
15, 26 -- Received: from c60.cesmail.net (c60.cesmail.net [216.154.195.49])
15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99GY2qp024267
15, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 11:34:02 -0500
15, 26 -- Received: from unknown (HELO delta2) ([192.168.1.50])
15, 26 -- by c60.cesmail.net with ESMTP; 09 Oct 2009 12:34:00 -0400
15, 26 -- Received: from pool-173-49-144-174.phlapa.fios.verizon.net
15, 26 -- (pool-173-49-144-174.phlapa.fios.verizon.net [173.49.144.174]) by
15, 26 -- webmail.spamcop.net (Horde MIME library) with HTTP; Fri, 09 Oct 2009
15, 26 -- 12:34:02 -0400
15, 26 -- Message-ID: {20091009123402.dqvt2ru1ecckkw0g-nzrark-at-webmail.spamcop.net}
15, 26 -- Date: Fri, 09 Oct 2009 12:34:02 -0400
15, 26 -- From: "George Langford, Sc.D." {amenex-at-spamcop.net}
15, 26 -- To: Microscopy-at-microscopy.com
15, 26 -- Cc: "George Langford, Sc.D." {amenex-at-amenex.com}
15, 26 -- Subject: Setting up legacy digital microscope cameras on more recent
15, 26 -- computers
15, 26 -- MIME-Version: 1.0
15, 26 -- Content-Type: text/plain;
15, 26 -- charset=ISO-8859-1;
15, 26 -- DelSp="Yes";
15, 26 -- format="flowed"
15, 26 -- Content-Disposition: inline
15, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4)
15, 26 -- Content-Transfer-Encoding: 8bit
15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n99GY2qp024267
==============================End of - Headers==============================




From: maryflet-at-interchange.ubc.ca
Date: Fri, 9 Oct 2009 12:18:41 -0500
Subject: [Microscopy] Cleaning aluminum parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Debbie,
They should be polished using metal polish, such as Wenol. If that does not
remove the black oxide, then you should grind them on fine (600 grit) emery
paper first, then follow with metal or diamond (6 micron) polish.
Regards,

Mary Fletcher
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca

-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: October 9, 2009 6:45 AM
To: maryflet-at-interchange.ubc.ca

Unfortunately some aluminum planchettes used in high pressure freezing were
left in water containing detergent for a few hours before cleaning was
completed. As a result the planchettes turned dark in color.

It is much more difficult to work with them in this condition. Does anyone
know a way to remove the tarnish and make them nice and shiny again?

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy





==============================Original Headers==============================
9, 31 -- From dsherman-at-purdue.edu Fri Oct 9 08:36:45 2009
9, 31 -- Received: from mailhub130.itcs.purdue.edu
(mailhub130.itcs.purdue.edu [128.210.5.130])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n99DajH5002451
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 08:36:45
-0500
9, 31 -- Received: from mailhub126.itcs.purdue.edu
(mailhub126.itcs.purdue.edu [128.210.5.126])
9, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n99DajKA007535
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45
-0400
9, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu
[172.21.6.92])
9, 31 -- by mailhub126.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n99DajKp012208
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45
-0400
9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.16]) by
WPPEXHUB03H.purdue.lcl
9, 31 -- ([172.21.6.92]) with mapi; Fri, 9 Oct 2009 09:36:44 -0400
9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
9, 31 -- Date: Fri, 9 Oct 2009 09:36:41 -0400
9, 31 -- Subject: Cleaning aluminum parts
9, 31 -- Thread-Topic: Cleaning aluminum parts
9, 31 -- Thread-Index: AcpI5YiahaNevBxLQ1qYopsj7xAsxA==
9, 31 -- Message-ID: {C6F4B4A9.13DB%dsherman-at-purdue.edu}
9, 31 -- Accept-Language: en-US
9, 31 -- Content-Language: en-US
9, 31 -- X-MS-Has-Attach:
9, 31 -- X-MS-TNEF-Correlator:
9, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
9, 31 -- acceptlanguage: en-US
9, 31 -- Content-Type: text/plain; charset="us-ascii"
9, 31 -- MIME-Version: 1.0
9, 31 -- X-PMX-Version: 5.5.7.378829
9, 31 -- X-PerlMx-Virus-Scanned: Yes
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n99DajH5002451
==============================End of - Headers==============================


==============================Original Headers==============================
17, 33 -- From maryflet-at-interchange.ubc.ca Fri Oct 9 12:18:41 2009
17, 33 -- Received: from mr7.mail-relay.ubc.ca (mr7.mail-relay.ubc.ca [137.82.45.13])
17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n99HIeIa008073
17, 33 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 12:18:41 -0500
17, 33 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70])
17, 33 -- by mr7.mail-relay.ubc.ca (Postfix) with ESMTP id 180A51C218
17, 33 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 10:18:39 -0700 (PDT)
17, 33 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178])
17, 33 -- by smtp.interchange.ubc.ca
17, 33 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
17, 33 -- with ESMTP id {0KR9009DKC3121-at-smtp.interchange.ubc.ca} for
17, 33 -- microscopy-at-microscopy.com; Fri, 09 Oct 2009 10:18:38 -0700 (PDT)
17, 33 -- Date: Fri, 09 Oct 2009 10:17:36 -0700
17, 33 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca}
17, 33 -- Subject: RE: [Microscopy] Cleaning aluminum parts
17, 33 -- In-reply-to: {200910091344.n99Din55016891-at-ns.microscopy.com}
17, 33 -- To: dsherman-at-purdue.edu
17, 33 -- Cc: microscopy-at-microscopy.com
17, 33 -- Reply-to: maryflet-at-interchange.ubc.ca
17, 33 -- Message-id: {0KR9009DLC3121-at-smtp.interchange.ubc.ca}
17, 33 -- Organization: Materials Eng.
17, 33 -- MIME-version: 1.0
17, 33 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
17, 33 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
17, 33 -- Content-type: text/plain; charset=us-ascii
17, 33 -- Content-transfer-encoding: 7bit
17, 33 -- Thread-index: AcpI5qyq/vWAUfUKSt+dF6Z0Ub3FNwAHWspA
17, 33 -- X-UBC-Scanned: Sophos PureMessage 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.10.9.170623
17, 33 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
17, 33 -- X-PerlMx-Spam: Probability=8%, Report=
17, 33 -- BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __USER_AGENT_MS_GENERIC 0
17, 33 -- X-Spam-Level:
17, 33 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Sat, 10 Oct 2009 21:37:39 -0500
Subject: [Microscopy] looking for images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,


I hope this email finds all of you in best of health and happiness.

A progressive engineering and biology school in north India aspires to upgrade its facilities, bring its students to the forefront and fill the lacunae being faced by its researchers by making the facility of Electron Microscopy available.

Unfortunately, the management cannot afford to purchase a brand new EM and related instruments, but is ready to bear all costs involved in shipping. Through this forum, I, on the behalf of the management, staff and students, put a humble request to the scientists and institutions to keep in their kind reference this college, if they wish to donate their old electron microscopes (in working condition) at present or in future.

The donators would be duly acknowledged along with their photographs on the school's website.

Lets join hands to add a new chapter in the book of the developing nations' striving for excellence.


Kindest regards,

Gill

--
Room No. 330,
Physical Sciences Building,
The Flinders University of SA,
Sturt Road, SA 5042
AUSTRALIA
Mobile: +61 425 759 100
Email: gill0245-at-flinders.edu.au


Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew


==============================Original Headers==============================
5, 22 -- From inderpreetsgill-at-yahoo.co.in Fri Oct 9 19:30:30 2009
5, 22 -- Received: from web8407.mail.in.yahoo.com (web8407.mail.in.yahoo.com [202.43.219.155])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9A0USXV011793
5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 19:30:29 -0500
5, 22 -- Received: (qmail 40751 invoked by uid 60001); 10 Oct 2009 00:30:28 -0000
5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.co.in; s=s1024; t=1255134628; bh=YolOX3gNO6VF2qDFgv9/iPkGsRX+STrgvbCmzsfuYjs=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=mPWKqJSIlOQQXqppQDAcpC/w+t9y7tT04RqzQLHdxarwcwHUNAtGlAcSmSe74nxmdtAB8W8majjx7YCHRx5JE7CcqzClUkh+2+CDp9tMECH6uBgJ39go3kYI7wJXa3jCVtakHiph5g5BGk0jWIMAazfU99G8WfoGSL+P5+emtdw=
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=yahoo.co.in;
5, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
5, 22 -- b=Z/Avp8H4wmy0E5SgBJx1BLo/J8OzE5RWoI9Mp35Z4CBJ7dZmk8KcnfYKAwNKY6JYhbwrz0ITEjXNC0H6Ho9HE07p+mM/53kf5zs8UWwxLVoYoHWOZD3IXFkW+1eIKYwEACqinu0aoGhNT2YhMqVq2yGUPzE5VPP7TgwlawYtHyc=;
5, 22 -- Message-ID: {41146.35548.qm-at-web8407.mail.in.yahoo.com}
5, 22 -- X-YMail-OSG: i3DLQW8VM1kGWyTUO2hQRvflVax5TLMd83diO3YhJCOXWEK31Xw9Y5Cgf9mOs8toejhVxGri7f4zvSCsXGu1T0vja_QdjsX1SQblQk1TMpou92fWwE3knY6aPZvW_FCdwt5i8HIkKHgH0IHJf_5fK2fmKsiou0nvg5fgbotI4Dio2vhxr6PQxWceg_gG2J8DjD.qqcs9tDNCLqcvEYMcgNmIipfXwxL9jPEqCzc_uHeaF92rnM2xlmH8Ie8bK7Jk1vFkXPCdG4jSwi5dJzpQL6bBMboicxHgkEhjAFwhAksmOOty5Fn6vBlj89mHWPrYpQcbxmE-
5, 22 -- Received: from [129.96.208.155] by web8407.mail.in.yahoo.com via HTTP; Sat, 10 Oct 2009 06:00:27 IST
5, 22 -- X-Mailer: YahooMailClassic/7.0.14 YahooMailWebService/0.7.347.3
5, 22 -- Date: Sat, 10 Oct 2009 06:00:27 +0530 (IST)
5, 22 -- From: INDERPREET GILL {inderpreetsgill-at-yahoo.co.in}
5, 22 -- Subject: Requirement for used SEM, TEM, AFM and sample preparation equipment
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=utf-8
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9A0USXV011793
==============================End of - Headers==============================

From w_martin23-at-kusndu.com Sat Oct 10 08:03:48 2009
Return-Path: {w_martin23-at-kusndu.com}
Received: from n54a.bullet.mail.sp1.yahoo.com (n54a.bullet.mail.sp1.yahoo.com [98.136.45.1])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9AD3ltu006927
for {microscopylistserverarchive-at-microscopy.com} ; Sat, 10 Oct 2009 08:03:48 -0500
Received: from [216.252.122.217] by n54.bullet.mail.sp1.yahoo.com with NNFMP; 10 Oct 2009 13:03:47 -0000
Received: from [69.147.84.34] by t2.bullet.sp1.yahoo.com with NNFMP; 10 Oct 2009 13:03:47 -0000
Received: from [127.0.0.1] by omp210.mail.sp1.yahoo.com with NNFMP; 10 Oct 2009 13:03:47 -0000
X-Yahoo-Newman-Property: ymail-5
X-Yahoo-Newman-Id: 649927.28398.bm-at-omp210.mail.sp1.yahoo.com
Received: (qmail 62330 invoked by uid 60001); 10 Oct 2009 13:03:44 -0000
Message-ID: {740552.55410.qm-at-web1202.biz.mail.gq1.yahoo.com}
X-YMail-OSG: fhtQB18VM1lzVsJgy7btUlhEntF04IHUzbDeUCd8oY0rNPIDKEtpRUG8RgUYBPD93taNkwne2x5BCckDe_PQ8ocxC4FS1wlBqfKcxpXnnFKa8HFCbxEVl_F6sQ9ibgd6JcaqDWXaFsvLLyOGvG8g_dute0v.6L_Yfg2eJAdj2XCDT4pSAJXlJC6dHg--
Received: from [86.62.27.210] by web1202.biz.mail.gq1.yahoo.com via HTTP; Sat, 10 Oct 2009 06:03:44 PDT
X-Mailer: YahooMailClassic/7.0.14 YahooMailWebService/0.7.347.3

Hello all,

Does anyone have good images of an ARL-EMX, ARL-SEMQ, and/or Cameca
MBX that they could email me? I'd like to show examples of these
instruments in a lecture, but I've only found relatively small images
online.

Thanks,
Ellery

-----------------
Ellery Frahm
Manager & Principal Analyst, Electron Microprobe Lab
Senior Research Fellow, Department of Geology & Geophysics
University of Minnesota - Twin Cities
http://probelab.geo.umn.edu

==============================Original Headers==============================
4, 17 -- From frah0010-at-umn.edu Sat Oct 10 21:37:39 2009
4, 17 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232])
4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9B2bdpn029659
4, 17 -- for {microscopy-at-microscopy.com} ; Sat, 10 Oct 2009 21:37:39 -0500
4, 17 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206])
4, 17 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP
4, 17 -- for {microscopy-at-microscopy.com} ; Sat, 10 Oct 2009 21:37:38 -0500 (CDT)
4, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN
4, 17 -- Message-Id: {07272513-E054-4CEF-8084-7242FB78E524-at-umn.edu}
4, 17 -- From: Ellery Frahm {frah0010-at-umn.edu}
4, 17 -- To: microscopy-at-microscopy.com
4, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 17 -- Content-Transfer-Encoding: 7bit
4, 17 -- Mime-Version: 1.0 (Apple Message framework v936)
4, 17 -- Subject: looking for images
4, 17 -- Date: Sat, 10 Oct 2009 21:37:37 -0500
4, 17 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Mon, 12 Oct 2009 05:00:08 -0500
Subject: [Microscopy] Sample for astigmatismus correction (REM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

As biologists we usually deal with poorly contrasted/bad conductive material in REM and they are not optimal for astigmatismus correction.
Would you have advises as what to use, whether a commercial or a make-it-yourself sample to correct astigmatismus up to 50 000x in REM?
Thanks in advance,

Stephane




==============================Original Headers==============================
5, 20 -- From nizets2-at-yahoo.com Mon Oct 12 05:00:08 2009
5, 20 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9CA07Rk030926
5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 05:00:07 -0500
5, 20 -- Received: (qmail 32042 invoked by uid 60001); 12 Oct 2009 10:00:07 -0000
5, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1255341606; bh=odmzWB7vGuHxYQPZbJtfYUnB8Tkzzgi35DYHojNQr4U=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=Fof4mEsdMxFedEBNaa1ZRHos0/DOrMXg7v7wjc29Vu/9bbFjGkFMuLJHNuHXSxX4xhqKYCjkxDuuNHzU99j1k6vAEmkmc8FDOYpEqbuzplrcxeVcXOrBaXpNzu+R03cHk0YNMJKIeFjuzk3/PPKD2+ffC696eLo8NbEF+aLKcY4=
5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 20 -- s=s1024; d=yahoo.com;
5, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
5, 20 -- b=UmP13pt49kCLcNYE2cWXsdorAg5p7H72GpivWPBdFXYZEKv7KdMrHIbR4XbRFuQFyEJScxdbq9bE46tWGqv27Gkda413Qdo9PtnrfrHCVXPSndWedXBUnkGxiQF0M2WbJntK+WjPkzhDd0NQMh3hKks+bwGZFmjRueCqkcwyTYk=;
5, 20 -- Message-ID: {272615.31462.qm-at-web110805.mail.gq1.yahoo.com}
5, 20 -- X-YMail-OSG: xVYmQjsVM1mnXGaZ2mQ1GRU88ekMhLLsoqe6je8IhL_OjNHpa76EGdhx9y8epn5W8HxqUXgQ8kvEmJDnbIC22sthfjwcXnelaV_HNxDmmYkNBaejETZG8VolKY0wBRqUp.8Y.5Oyv8LhZASSh7uT19L59fX48TsY7IjnSagMi3rpOje95U9B23zuPopxU46sklZLWi8bcTtZ5zsdYnXcbJ5PaN_rSEjbdmLGBumm2KiMO9VbQT68IDSwlxdF5XBlyU.fJDPxsJbkSRRlZV7uK4_kLcxRGKhGrYpa2ZInNeh1gfE2PVQuO4rUUqcWk3_F2zZIDhOpTMY2Cmjb2KWFFqk-
5, 20 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Mon, 12 Oct 2009 03:00:06 PDT
5, 20 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
5, 20 -- Date: Mon, 12 Oct 2009 03:00:06 -0700 (PDT)
5, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 20 -- Subject: Sample for astigmatismus correction (REM)
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Mon, 12 Oct 2009 06:09:09 -0500
Subject: [Microscopy] photomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I normally don't forward web sites to this list..... but the images are
wonderful. If you enjoy photomicroscopy, visit the wired website soon.
here's the link:
http://www.wired.com/wiredscience/2009/10/photomicrography/all/1

Stay safe.....
Frank

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
5, 21 -- From frank_karl-at-lincolnelectric.com Mon Oct 12 06:09:09 2009
5, 21 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9CB99am019634
5, 21 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 06:09:09 -0500
5, 21 -- Subject: photomicroscopy
5, 21 -- To: Microscopy-at-microscopy.com, "Karen Karl" {k1karl-at-yahoo.com}
5, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
5, 21 -- Message-ID: {OF53AD4D33.22656D29-ON8525764D.003D10DC-8525764D.003D370D-at-lincolnelectric.com}
5, 21 -- Date: Mon, 12 Oct 2009 07:09:11 -0400
5, 21 -- From: Frank_Karl-at-lincolnelectric.com
5, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 21 -- 07, 2008) at 10/12/2009 07:08:42 AM,
5, 21 -- CD-MIME complete at 10/12/2009 07:08:42 AM,
5, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 21 -- 07, 2008) at 10/12/2009 07:08:42 AM,
5, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
5, 21 -- 07, 2008) at 10/12/2009 07:08:42 AM,
5, 21 -- Serialize complete at 10/12/2009 07:08:42 AM
5, 21 -- MIME-Version: 1.0
5, 21 -- Content-Type: text/plain;
5, 21 -- charset="US-ASCII"
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Mon, 12 Oct 2009 08:06:31 -0500
Subject: [Microscopy] Cleaning aluminum parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,
I believe a dip in nitric acid will clean them up. Maybe Woody White can
chime in here about concentration and length of time. He originally told me
about this.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Friday, October 09, 2009 9:39 AM
To: kenconverse-at-qualityimages.biz

Unfortunately some aluminum planchettes used in high pressure freezing were
left in water containing detergent for a few hours before cleaning was
completed. As a result the planchettes turned dark in color.

It is much more difficult to work with them in this condition. Does anyone
know a way to remove the tarnish and make them nice and shiny again?

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy





==============================Original Headers==============================
9, 31 -- From dsherman-at-purdue.edu Fri Oct 9 08:36:45 2009
9, 31 -- Received: from mailhub130.itcs.purdue.edu
(mailhub130.itcs.purdue.edu [128.210.5.130])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n99DajH5002451
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 08:36:45
-0500
9, 31 -- Received: from mailhub126.itcs.purdue.edu
(mailhub126.itcs.purdue.edu [128.210.5.126])
9, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n99DajKA007535
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45
-0400
9, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu
[172.21.6.92])
9, 31 -- by mailhub126.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n99DajKp012208
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009 09:36:45
-0400
9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.16]) by
WPPEXHUB03H.purdue.lcl
9, 31 -- ([172.21.6.92]) with mapi; Fri, 9 Oct 2009 09:36:44 -0400
9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
9, 31 -- Date: Fri, 9 Oct 2009 09:36:41 -0400
9, 31 -- Subject: Cleaning aluminum parts
9, 31 -- Thread-Topic: Cleaning aluminum parts
9, 31 -- Thread-Index: AcpI5YiahaNevBxLQ1qYopsj7xAsxA==
9, 31 -- Message-ID: {C6F4B4A9.13DB%dsherman-at-purdue.edu}
9, 31 -- Accept-Language: en-US
9, 31 -- Content-Language: en-US
9, 31 -- X-MS-Has-Attach:
9, 31 -- X-MS-TNEF-Correlator:
9, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
9, 31 -- acceptlanguage: en-US
9, 31 -- Content-Type: text/plain; charset="us-ascii"
9, 31 -- MIME-Version: 1.0
9, 31 -- X-PMX-Version: 5.5.7.378829
9, 31 -- X-PerlMx-Virus-Scanned: Yes
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n99DajH5002451
==============================End of - Headers==============================




==============================Original Headers==============================
21, 25 -- From kenconverse-at-qualityimages.biz Mon Oct 12 08:06:31 2009
21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9CD6TtP008762
21, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 08:06:31 -0500
21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
21, 25 -- with ESMTP
21, 25 -- id {20091012130627700.XRWO16663-at-cdptpa-omta01.mail.rr.com} ;
21, 25 -- Mon, 12 Oct 2009 13:06:27 +0000
21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 25 -- To: {dsherman-at-purdue.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
21, 25 -- Subject: RE: [Microscopy] Cleaning aluminum parts
21, 25 -- Date: Mon, 12 Oct 2009 09:06:19 -0400
21, 25 -- Message-ID: {9AB76387B3704F05BC8870475EAC8253-at-Ken}
21, 25 -- MIME-Version: 1.0
21, 25 -- Content-Type: text/plain;
21, 25 -- charset="US-ASCII"
21, 25 -- X-Priority: 3 (Normal)
21, 25 -- X-MSMail-Priority: Normal
21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 25 -- Importance: Normal
21, 25 -- Thread-Index: AcpI5epa5v54l5kqS8KFrAJmStRRFQCVpI8g
21, 25 -- In-Reply-To: {200910091339.n99DdPJQ006353-at-ns.microscopy.com}
21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 25 -- Content-Transfer-Encoding: 8bit
21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9CD6TtP008762
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Mon, 12 Oct 2009 08:20:19 -0500
Subject: [Microscopy] Sample for astigmatismus correction (REM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,
Having a specific sample for correcting astigmatism is not going to help,
beyond finding out what the intrinsic astigmatism is in the column. Any
sample that charges is going to have varying astigmatism depending upon the
exact area scanned (for those who are not familiar with REM, it is raster
electron microscope, otherwise known as SEM), the beam current, the length
of time scanned, and the scan rate (especially the horizontal scan rate).

Generally speaking, reducing the accelerating voltage, reducing the beam
current, and increasing the horizontal scan speed will reduce charging and
the associated astigmatism. One thing that can help is to focus and
stigmate carefully on an area very close to where you want to collect your
image, then shift the field of view, quickly make any minor corrections and
take the picture.

The bottom line is this: If you haven't stigmated, you haven't focused.
Stigmating the beam is an integral part of focusing and cannot just be set
once and forgotten. It must be done EVERY time you want a good image,
especially at high magnifications.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Monday, October 12, 2009 6:03 AM
To: kenconverse-at-qualityimages.biz

Dear listers,

As biologists we usually deal with poorly contrasted/bad conductive material
in REM and they are not optimal for astigmatismus correction.
Would you have advises as what to use, whether a commercial or a
make-it-yourself sample to correct astigmatismus up to 50 000x in REM?
Thanks in advance,

Stephane




==============================Original Headers==============================
5, 20 -- From nizets2-at-yahoo.com Mon Oct 12 05:00:08 2009
5, 20 -- Received: from web110805.mail.gq1.yahoo.com
(web110805.mail.gq1.yahoo.com [67.195.13.228])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n9CA07Rk030926
5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 05:00:07
-0500
5, 20 -- Received: (qmail 32042 invoked by uid 60001); 12 Oct 2009 10:00:07
-0000
5, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1255341606; bh=odmzWB7vGuHxYQPZbJtfYUnB8Tkzzgi35DYHojNQr4U=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
b=Fof4mEsdMxFedEBNaa1ZRHos0/DOrMXg7v7wjc29Vu/9bbFjGkFMuLJHNuHXSxX4xhqKYCjkxD
uuNHzU99j1k6vAEmkmc8FDOYpEqbuzplrcxeVcXOrBaXpNzu+R03cHk0YNMJKIeFjuzk3/PPKD2+
ffC696eLo8NbEF+aLKcY4=
5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 20 -- s=s1024; d=yahoo.com;
5, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version
:Content-Type;
5, 20 --
b=UmP13pt49kCLcNYE2cWXsdorAg5p7H72GpivWPBdFXYZEKv7KdMrHIbR4XbRFuQFyEJScxdbq9
bE46tWGqv27Gkda413Qdo9PtnrfrHCVXPSndWedXBUnkGxiQF0M2WbJntK+WjPkzhDd0NQMh3hKk
s+bwGZFmjRueCqkcwyTYk=;
5, 20 -- Message-ID: {272615.31462.qm-at-web110805.mail.gq1.yahoo.com}
5, 20 -- X-YMail-OSG:
xVYmQjsVM1mnXGaZ2mQ1GRU88ekMhLLsoqe6je8IhL_OjNHpa76EGdhx9y8epn5W8HxqUXgQ8kvE
mJDnbIC22sthfjwcXnelaV_HNxDmmYkNBaejETZG8VolKY0wBRqUp.8Y.5Oyv8LhZASSh7uT19L5
9fX48TsY7IjnSagMi3rpOje95U9B23zuPopxU46sklZLWi8bcTtZ5zsdYnXcbJ5PaN_rSEjbdmLG
Bumm2KiMO9VbQT68IDSwlxdF5XBlyU.fJDPxsJbkSRRlZV7uK4_kLcxRGKhGrYpa2ZInNeh1gfE2
PVQuO4rUUqcWk3_F2zZIDhOpTMY2Cmjb2KWFFqk-
5, 20 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via
HTTP; Mon, 12 Oct 2009 03:00:06 PDT
5, 20 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
5, 20 -- Date: Mon, 12 Oct 2009 03:00:06 -0700 (PDT)
5, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 20 -- Subject: Sample for astigmatismus correction (REM)
5, 20 -- To: microscopy-at-microscopy.com
5, 20 -- MIME-Version: 1.0
5, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




==============================Original Headers==============================
19, 25 -- From kenconverse-at-qualityimages.biz Mon Oct 12 08:20:19 2009
19, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9CDKJud023767
19, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 08:20:19 -0500
19, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com
19, 25 -- with ESMTP
19, 25 -- id {20091012132017727.QHFH14562-at-cdptpa-omta03.mail.rr.com} ;
19, 25 -- Mon, 12 Oct 2009 13:20:17 +0000
19, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
19, 25 -- To: {nizets2-at-yahoo.com} , "MSA Listserver" {microscopy-at-microscopy.com}
19, 25 -- Subject: RE: [Microscopy] Sample for astigmatismus correction (REM)
19, 25 -- Date: Mon, 12 Oct 2009 09:20:09 -0400
19, 25 -- Message-ID: {7D2564461C28452489FECCA3B439FE60-at-Ken}
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Type: text/plain;
19, 25 -- charset="US-ASCII"
19, 25 -- X-Priority: 3 (Normal)
19, 25 -- X-MSMail-Priority: Normal
19, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
19, 25 -- Importance: Normal
19, 25 -- Thread-Index: AcpLIzZGkIbtScdnQR+UY4Rpsxd6hwAGdmwQ
19, 25 -- In-Reply-To: {200910121003.n9CA3ENe002578-at-ns.microscopy.com}
19, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9CDKJud023767
==============================End of - Headers==============================




From: wesaia-at-iastate.edu
Date: Mon, 12 Oct 2009 10:02:24 -0500
Subject: [Microscopy] Sample for astigmatismus correction (REM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am often asked to examine some fairly smooth surfaces for their fine
structure. Often, the sample seems so featureless that I cannot easily
focus. In those cases, I take one of two approaches.

In one scenario, I start on the edge of the sample and do as much
focusing and astigmatism correction as possible. Corners are usually
better than edges since it is hard to correct for astigmatism with a
single straight edge.

The other scenario is a judicious level of uncleanliness. A little bit
of dust can be a good thing as it provides something to focus on.
Certainly a lot of dirt or debris could obscure the important features
or foul an otherwise good image. However, a small dust particle in the
neighborhood of a feature of interest is preferable to an edge hundreds
of microns away.

For what it is worth,
Warren

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Monday, October 12, 2009 5:01 AM
To: wesaia-at-iastate.edu

Dear listers,

As biologists we usually deal with poorly contrasted/bad conductive
material in REM and they are not optimal for astigmatismus correction.
Would you have advises as what to use, whether a commercial or a
make-it-yourself sample to correct astigmatismus up to 50 000x in REM?
Thanks in advance,

Stephane



==============================Original Headers==============================
10, 36 -- From wesaia-at-iastate.edu Mon Oct 12 10:02:24 2009
10, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9CF2OMN010878
10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 10:02:24 -0500
10, 36 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
10, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n9CF2NxA031906
10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 10:02:23 -0500
10, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
10, 36 -- id 0ad6_3f7d8fae_b740_11de_8cb6_00137253420a;
10, 36 -- Mon, 12 Oct 2009 10:02:23 -0500
10, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
10, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n9CF2N4Z008711
10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 10:02:23 -0500
10, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 36 -- Mon, 12 Oct 2009 10:02:03 -0500
10, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 36 -- Content-class: urn:content-classes:message
10, 36 -- MIME-Version: 1.0
10, 36 -- Content-Type: text/plain;
10, 36 -- charset="US-ASCII"
10, 36 -- Subject: RE: [Microscopy] Sample for astigmatismus correction (REM)
10, 36 -- Date: Mon, 12 Oct 2009 10:02:29 -0500
10, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270444AD0F-at-maire.eng.iastate.edu}
10, 36 -- In-Reply-To: {200910121001.n9CA17Lw032007-at-ns.microscopy.com}
10, 36 -- X-MS-Has-Attach:
10, 36 -- X-MS-TNEF-Correlator:
10, 36 -- Thread-Topic: [Microscopy] Sample for astigmatismus correction (REM)
10, 36 -- Thread-Index: AcpLIuw9VMw814+iT2OW/0QunmPdTwAKP+Mg
10, 36 -- References: {200910121001.n9CA17Lw032007-at-ns.microscopy.com}
10, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
10, 36 -- To: {Microscopy-at-microscopy.com}
10, 36 -- X-OriginalArrivalTime: 12 Oct 2009 15:02:03.0911 (UTC) FILETIME=[F555A970:01CA4B4C]
10, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.10.12.145121
10, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1700_1799 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0'
10, 36 -- Content-Transfer-Encoding: 8bit
10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9CF2OMN010878
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Mon, 12 Oct 2009 11:24:42 -0500
Subject: [Microscopy] Re: Sample for astigmatismus correction (REM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

REM=SEM.

Get a gold on carbon "standard" from Pella, et. al. or Agar.
Choose the gold particle size to be compatable with your
magnification and resolution. 50KX is not bad. The trick
is to get some large chunks of gold along with small ones
in between.

This is not going to solve your stig situation since each
beam condition and specimen need custom stigmation. But what
the gold on carbon will tell you is whether your column needs
alignment or your apertures are dirty.

For bio samples, you have to find a discerning feature and
stig on that. Not always easy.

gary g.


At 03:02 AM 10/12/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 20 -- From gary-at-gaugler.com Mon Oct 12 11:24:42 2009
11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9CGOgYi030081
11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009 11:24:42 -0500
11, 20 -- Message-Id: {200910121624.n9CGOgYi030081-at-ns.microscopy.com}
11, 20 -- Received: (qmail 6455 invoked from network); 12 Oct 2009 09:58:43 -0700
11, 20 -- Received: by simscan 1.1.0 ppid: 6450, pid: 6451, t: 0.1041s
11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
11, 20 -- by smtp1 with SMTP; 12 Oct 2009 09:58:43 -0700
11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
11, 20 -- Date: Mon, 12 Oct 2009 09:24:33 -0700
11, 20 -- To: nizets2-at-yahoo.com
11, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 20 -- Subject: Re: [Microscopy] Sample for astigmatismus correction (REM)
11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 20 -- In-Reply-To: {200910121002.n9CA25EO000951-at-ns.microscopy.com}
11, 20 -- References: {200910121002.n9CA25EO000951-at-ns.microscopy.com}
11, 20 -- Mime-Version: 1.0
11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Tue, 13 Oct 2009 00:37:02 -0500
Subject: [Microscopy] Hitachi S4300 SE/N beam shape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have noticed a small "quirk" in the beam shape in our Hitachi S4300 SE/N. When you align the beam, occasionally the beam shape appears elliptical. If you switch from beam align mode to normal imaging mode and then back to beam align mode the beam will then appear circular. Sometimes it is necessary to repeat this a couple of times before the beam returns to its circular shape.

It seems to be independent of samples (either what was in the microscope previously or is currently there) and totally random in its timing. We look at a wide range of materials, from natural fibres, carbon nanotubes metals and semiconductors and there is no correlation between sample and beam shape.

The ellipse always has its major axis in the same direction, just a few degrees left of vertical. Also, the ellipticity is present whether or not there is an objective aperture in place. If it is present it will be present for all apertures.

There is a degauss option in the software but this has no effect either.

Once settled there doesn't seem to be any degradation of the performance of the microscope.

Any help or clues as to what may be happening would be greatly appreciated.

Cheers

Colin Veitch

Electron Microscopist
CSIRO Materials Science and Engineering, Geelong Laboratory
PO Box 21, BELMONT, Vic. 3216. Australia.

colin.veitch-at-csiro.au
http://www.tft.csiro.au

Tel:       +61 (0) 3 5246 4000  
Mobile:  0438 538 475
Fax:      +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.



==============================Original Headers==============================
15, 46 -- From prvs=53063e716=Colin.Veitch-at-csiro.au Tue Oct 13 00:37:02 2009
15, 46 -- Received: from vic-MTAout5.csiro.au (vic-MTAout5.csiro.au [150.229.64.42])
15, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9D5b0lM030015
15, 46 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 00:37:01 -0500
15, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
15, 46 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt;
15, 46 -- s=email; t=1255412222; x=1286948222;
15, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
15, 46 -- mime-version:content-transfer-encoding:content-id:
15, 46 -- content-description:resent-date:resent-from:resent-sender:
15, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
15, 46 -- references:list-id:list-help:list-unsubscribe:
15, 46 -- list-subscribe:list-post:list-owner:list-archive;
15, 46 -- z=From:=20 {Colin.Veitch-at-csiro.au} |Subject:=20Hitachi=20S43
15, 46 -- 00=20SE/N=20beam=20shape|Date:=20Tue,=2013=20Oct=202009
15, 46 -- =2016:36:19=20+1100|Message-ID:=20 {4AA5DD81F68A934F8C80DA
15, 46 -- 2BF125163D3B983F5068-at-EXVIC-MBX02.nexus.csiro.au} |To:=20 {m
15, 46 -- icroscopy-at-microscopy.com} |MIME-Version:=201.0
15, 46 -- |Content-Transfer-Encoding:=20quoted-printable;
15, 46 -- bh=RC6+XJYJari0ctEoc3IltJ4+gz5QM/Pb8sqSS2jJxLA=;
15, 46 -- b=i9H8GyrTrzSH54ajbRhXjT4/qYZQ1KEy4OwtSt2BYt3sIkvXhSzYxfgy
15, 46 -- O/1fQlakz24vfhXUE1YoC6IV2CLSYt+L5XX2rsPicSgTXCNXzf+g/ClSW
15, 46 -- uv/WAD2B8uxbHDj;
15, 46 -- X-IronPort-AV: E=Sophos;i="4.44,550,1249221600";
15, 46 -- d="scan'208";a="24673999"
15, 46 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127])
15, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 13 Oct 2009 16:36:58 +1100
15, 46 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by
15, 46 -- exvic-htca02.nexus.csiro.au ([138.194.81.127]) with mapi; Tue, 13 Oct 2009
15, 46 -- 16:36:20 +1100
15, 46 -- From: {Colin.Veitch-at-csiro.au}
15, 46 -- To: {microscopy-at-microscopy.com}
15, 46 -- Date: Tue, 13 Oct 2009 16:36:19 +1100
15, 46 -- Subject: Hitachi S4300 SE/N beam shape
15, 46 -- Thread-Topic: Hitachi S4300 SE/N beam shape
15, 46 -- Thread-Index: AcpLxxclenHjPVbgR5WIPqawHihEmQ==
15, 46 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D3B983F5068-at-EXVIC-MBX02.nexus.csiro.au}
15, 46 -- Accept-Language: en-US, en-AU
15, 46 -- Content-Language: en-US
15, 46 -- X-MS-Has-Attach:
15, 46 -- X-MS-TNEF-Correlator:
15, 46 -- acceptlanguage: en-US, en-AU
15, 46 -- Content-Type: text/plain; charset="iso-8859-1"
15, 46 -- MIME-Version: 1.0
15, 46 -- Content-Transfer-Encoding: 8bit
15, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9D5b0lM030015
==============================End of - Headers==============================




From: ron.doole-at-materials.ox.ac.uk
Date: Tue, 13 Oct 2009 01:56:48 -0500
Subject: [Microscopy] Hitachi S4300 SE/N beam shape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Colin,

Does it vary with scan speed? I am wondering if it is caused by the signal lagging behind the display scan. Possible causes could be hysteresis or charging in the column.

Regards,
Ron



Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: Colin.Veitch-at-csiro.au [Colin.Veitch-at-csiro.au]
Sent: 13 October 2009 06:46
To: Ron Doole

Hi,

I have noticed a small "quirk" in the beam shape in our Hitachi S4300 SE/N. When you align the beam, occasionally the beam shape appears elliptical. If you switch from beam align mode to normal imaging mode and then back to beam align mode the beam will then appear circular. Sometimes it is necessary to repeat this a couple of times before the beam returns to its circular shape.

It seems to be independent of samples (either what was in the microscope previously or is currently there) and totally random in its timing. We look at a wide range of materials, from natural fibres, carbon nanotubes metals and semiconductors and there is no correlation between sample and beam shape.

The ellipse always has its major axis in the same direction, just a few degrees left of vertical. Also, the ellipticity is present whether or not there is an objective aperture in place. If it is present it will be present for all apertures.

There is a degauss option in the software but this has no effect either.

Once settled there doesn't seem to be any degradation of the performance of the microscope.

Any help or clues as to what may be happening would be greatly appreciated.

Cheers

Colin Veitch

Electron Microscopist
CSIRO Materials Science and Engineering, Geelong Laboratory
PO Box 21, BELMONT, Vic. 3216. Australia.

colin.veitch-at-csiro.au
http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mobile: 0438 538 475
Fax: +61 (0) 3 5246 4811

The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.



==============================Original Headers==============================
15, 46 -- From prvs=53063e716=Colin.Veitch-at-csiro.au Tue Oct 13 00:37:02 2009
15, 46 -- Received: from vic-MTAout5.csiro.au (vic-MTAout5.csiro.au [150.229.64.42])
15, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9D5b0lM030015
15, 46 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 00:37:01 -0500
15, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
15, 46 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt;
15, 46 -- s=email; t=1255412222; x=1286948222;
15, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
15, 46 -- mime-version:content-transfer-encoding:content-id:
15, 46 -- content-description:resent-date:resent-from:resent-sender:
15, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to:
15, 46 -- references:list-id:list-help:list-unsubscribe:
15, 46 -- list-subscribe:list-post:list-owner:list-archive;
15, 46 -- z=From:=20 {Colin.Veitch-at-csiro.au} |Subject:=20Hitachi=20S43
15, 46 -- 00=20SE/N=20beam=20shape|Date:=20Tue,=2013=20Oct=202009
15, 46 -- =2016:36:19=20+1100|Message-ID:=20 {4AA5DD81F68A934F8C80DA
15, 46 -- 2BF125163D3B983F5068-at-EXVIC-MBX02.nexus.csiro.au} |To:=20 {m
15, 46 -- icroscopy-at-microscopy.com} |MIME-Version:=201.0
15, 46 -- |Content-Transfer-Encoding:=20quoted-printable;
15, 46 -- bh=RC6+XJYJari0ctEoc3IltJ4+gz5QM/Pb8sqSS2jJxLA=;
15, 46 -- b=i9H8GyrTrzSH54ajbRhXjT4/qYZQ1KEy4OwtSt2BYt3sIkvXhSzYxfgy
15, 46 -- O/1fQlakz24vfhXUE1YoC6IV2CLSYt+L5XX2rsPicSgTXCNXzf+g/ClSW
15, 46 -- uv/WAD2B8uxbHDj;
15, 46 -- X-IronPort-AV: E=Sophos;i="4.44,550,1249221600";
15, 46 -- d="scan'208";a="24673999"
15, 46 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127])
15, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 13 Oct 2009 16:36:58 +1100
15, 46 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by
15, 46 -- exvic-htca02.nexus.csiro.au ([138.194.81.127]) with mapi; Tue, 13 Oct 2009
15, 46 -- 16:36:20 +1100
15, 46 -- From: {Colin.Veitch-at-csiro.au}
15, 46 -- To: {microscopy-at-microscopy.com}
15, 46 -- Date: Tue, 13 Oct 2009 16:36:19 +1100
15, 46 -- Subject: Hitachi S4300 SE/N beam shape
15, 46 -- Thread-Topic: Hitachi S4300 SE/N beam shape
15, 46 -- Thread-Index: AcpLxxclenHjPVbgR5WIPqawHihEmQ==
15, 46 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D3B983F5068-at-EXVIC-MBX02.nexus.csiro.au}
15, 46 -- Accept-Language: en-US, en-AU
15, 46 -- Content-Language: en-US
15, 46 -- X-MS-Has-Attach:
15, 46 -- X-MS-TNEF-Correlator:
15, 46 -- acceptlanguage: en-US, en-AU
15, 46 -- Content-Type: text/plain; charset="iso-8859-1"
15, 46 -- MIME-Version: 1.0
15, 46 -- Content-Transfer-Encoding: 8bit
15, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9D5b0lM030015
==============================End of - Headers==============================

==============================Original Headers==============================
23, 30 -- From ron.doole-at-materials.ox.ac.uk Tue Oct 13 01:56:48 2009
23, 30 -- Received: from relay3.mail.ox.ac.uk (relay3.mail.ox.ac.uk [163.1.2.165])
23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9D6ulaM014580
23, 30 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 01:56:47 -0500
23, 30 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk)
23, 30 -- by relay3.mail.ox.ac.uk with esmtp (Exim 4.69)
23, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
23, 30 -- id 1MxbJO-00037v-CE; Tue, 13 Oct 2009 07:56:46 +0100
23, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.1.8]) by
23, 30 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Tue, 13 Oct 2009 07:56:46
23, 30 -- +0100
23, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
23, 30 -- To: "Colin.Veitch-at-csiro.au" {Colin.Veitch-at-csiro.au}
23, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
23, 30 -- Date: Tue, 13 Oct 2009 07:56:45 +0100
23, 30 -- Subject: RE: [Microscopy] Hitachi S4300 SE/N beam shape
23, 30 -- Thread-Topic: [Microscopy] Hitachi S4300 SE/N beam shape
23, 30 -- Thread-Index: AcpLyHeBxOPvK/xdSp2qaYpmzL50oQACRj4k
23, 30 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF9264B8ACD11-at-EXMBX02.ad.oak.ox.ac.uk}
23, 30 -- References: {200910130546.n9D5k6ph012080-at-ns.microscopy.com}
23, 30 -- In-Reply-To: {200910130546.n9D5k6ph012080-at-ns.microscopy.com}
23, 30 -- Accept-Language: en-US, en-GB
23, 30 -- Content-Language: en-GB
23, 30 -- X-MS-Has-Attach:
23, 30 -- X-MS-TNEF-Correlator:
23, 30 -- acceptlanguage: en-US, en-GB
23, 30 -- Content-Type: text/plain; charset="us-ascii"
23, 30 -- MIME-Version: 1.0
23, 30 -- Content-Transfer-Encoding: 8bit
23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9D6ulaM014580
==============================End of - Headers==============================




From: j.janssen-at-nki.nl
Date: Tue, 13 Oct 2009 09:08:47 -0500
Subject: [Microscopy] viaWWW: Gatan cryo holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: j.janssen-at-nki.nl
Name: Hans Janssen

Organization: Netherlands Cancer Institute

Title-Subject: [Filtered] Gatan cryo holder

Question: We are looking for a second hand Gatan single tilt
cryoholder for the FEI Tecnai series. It doesn't matter whether this
is a 60 or 70 degrees tiltable holder. We like to have a second hand
one because we need to modify it to fit in a specialized Titan.

Login Host: 194.171.7.39
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Oct 13 09:08:47 2009
6, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9DE8iHd017963
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 09:08:45 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c6fa3a2fd653-at-[206.69.208.22]}
6, 11 -- Date: Tue, 13 Oct 2009 09:08:43 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Gatan cryo holder
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: vkrishn3-at-utk.edu
Date: Tue, 13 Oct 2009 09:09:28 -0500
Subject: [Microscopy] viaWWW: Cryomicrotoming hydrated NAFION film (178 microns thick)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both vkrishn3-at-utk.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: vkrishn3-at-utk.edu
Name: veena krishnan

Organization: UTK

Title-Subject: [Filtered] Cryomicrotoming hydrated NAFION film (178
microns thick)

Question: I am trying to do microscopic studies of hydrated NAFION
films. I wish to use a cryomicrotome to section these NAFIOn films. I
cannot use water to collect my sections. I am presently
cryomicrotoming the NAFION film at -80C and am using a diamond knife
to avoid flexing of the film. I have been trying to use the eyelash
tool to pick up my section and transfer it to the grid. But in doing
so my sections are rolling up and forming a tube instead of being
flat. The problem I am having is transfering the cut sections on the
diamond knife onto the TEM grid without destroying it. Any advice
will be highly appreciated.



Login Host: 160.36.234.57
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Oct 13 09:09:28 2009
8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9DE9RQZ018510
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 09:09:28 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c6fa3a77e75e-at-[206.69.208.22]}
8, 11 -- Date: Tue, 13 Oct 2009 09:09:26 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: vkrishn3-at-utk.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Cryomicrotoming hydrated NAFION film (178 microns thick)
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Tue, 13 Oct 2009 16:37:28 -0500
Subject: [Microscopy] Flushing advice?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

My JEOL 840 doesn't show any signs of scale buildup in its water-cooled internals at this
stage, but I would like very much to keep it that way rather than to have to deal with a
blockage and its consequences.

Anyone got any suggestions/advice on what to flush it out with to remove any scale that may
be building up?

I have a small portable tank-and-pump setup which I could use to pump a suitable flushing
solution through the 840 for anything from a few minutes to a few days.

It's cooled by a closed system which comprises a buffer tank, a chiller, and two circulation
pumps (one for the instruments, one for the chiller) and which serves the 840, an XRF, and a
couple of XRDs. The temperature is set to 20 deg C to avoid condensation problems, as it
gets pretty humid here at times.

The system is automatically topped up with mains tap water (we have very good clean soft
mains water here), but nothing is added to the water -- no biocide, no anti-corrosive, no
nuttin'.

In the ten years the setup has been operating I've not noticed any particular problems with
algae or corrosion, but I do wonder from time to time whether I should add something to the
water system.

Suggestions/advice solicited.

cheers
Ritchie Sims

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
11, 28 -- From r.sims-at-auckland.ac.nz Tue Oct 13 16:37:28 2009
11, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9DLbNc2000535
11, 28 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 16:37:28 -0500
11, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1])
11, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id BC80C9CB5A
11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 10:37:22 +1300 (NZDT)
11, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
11, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
11, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
11, 28 -- with ESMTP id ZblFjMx2fFhA for {microscopy-at-microscopy.com} ;
11, 28 -- Wed, 14 Oct 2009 10:37:22 +1300 (NZDT)
11, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
11, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
11, 28 -- (No client certificate requested)
11, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 9AC889CB4E
11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 10:37:22 +1300 (NZDT)
11, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
11, 28 -- To: microscopy-at-microscopy.com
11, 28 -- Date: Wed, 14 Oct 2009 10:41:03 +1300
11, 28 -- MIME-Version: 1.0
11, 28 -- Subject: Flushing advice?
11, 28 -- Message-ID: {4AD5AABF.20606.80230A-at-r.sims.auckland.ac.nz}
11, 28 -- Priority: normal
11, 28 -- X-mailer: Pegasus Mail for Windows (4.41)
11, 28 -- Content-type: text/plain; charset=US-ASCII
11, 28 -- Content-transfer-encoding: 7BIT
11, 28 -- Content-description: Mail message body
==============================End of - Headers==============================




From: marcello.serracino-at-igag.cnr.it
Date: Wed, 14 Oct 2009 07:37:49 -0500
Subject: [Microscopy] viaWWW: Crystalbond

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both marcello.serracino-at-igag.cnr.it as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: marcello.serracino-at-igag.cnr.it
Name: Marcello Serracino

Organization: National Research Council CNR-Italy

Title-Subject: [Filtered] Crystalbond

Question: Does anyone know if crystalbond embedding samples can
affect or contaminate the microprobe?
I would appreciate any suggestion.
Best Regards
Marcello Serracino

Login Host: 151.100.51.18
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Oct 14 07:37:49 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9ECbmXZ031411
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 07:37:48 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c6fb768bfd4f-at-[206.69.208.22]}
6, 11 -- Date: Wed, 14 Oct 2009 07:37:47 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: marcello.serracino-at-igag.cnr.it (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Crystalbond
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 14 Oct 2009 13:52:16 -0500
Subject: [Microscopy] Replacement Pe gauge for DSM 962

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, All-

I have a colleague who needs a replacement Penning gauge for a Zeiss DSM
962 SEM. The gauge is a Balzers IKR-020. Actually, what he really needs
is the anode, BG 510 999 X Anode. Any ideas? He says he struck out with
Zeiss and searching on the Web.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
5, 19 -- From tina-at-pbrc.hawaii.edu Wed Oct 14 13:52:16 2009
5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9EIqFOb030432
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 13:52:15 -0500
5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n9EIq96q002054
5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 08:52:11 -1000 (HST)
5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n9EIq8UA002051
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 08:52:09 -1000 (HST)
5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
5, 19 -- Date: Wed, 14 Oct 2009 08:52:08 -1000 (HST)
5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
5, 19 -- X-Sender: tina-at-halia
5, 19 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
5, 19 -- Subject: Replacement Pe gauge for DSM 962
5, 19 -- Message-ID: {Pine.GSO.4.21.0910140848290.1960-100000-at-halia}
5, 19 -- MIME-Version: 1.0
5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: underwoo-at-u.washington.edu
Date: Wed, 14 Oct 2009 14:54:47 -0500
Subject: [Microscopy] Re: viaWWW: Cryomicrotoming hydrated NAFION film (178

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Veena,

I'm not sure why you can't pick the sections up using a wire loop containing a droplet of buffered sucrose or methylcellulose/sucrose. Does this destroy the film? If not this would be the easiest way of transferring the sections to a grid. However, if this is not an option it may be easier to flatten the sections out with the eyelash then place the grid down on top of the sections. Hopefully a magical static charge will adhere the sections to the grid. This may require adjusting or turn off the anti-static device.

Bob Underwood


On Tue, 13 Oct 2009 vkrishn3-at-utk.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both vkrishn3-at-utk.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: vkrishn3-at-utk.edu
} Name: veena krishnan
}
} Organization: UTK
}
} Title-Subject: [Filtered] Cryomicrotoming hydrated NAFION film (178
} microns thick)
}
} Question: I am trying to do microscopic studies of hydrated NAFION
} films. I wish to use a cryomicrotome to section these NAFIOn films. I
} cannot use water to collect my sections. I am presently
} cryomicrotoming the NAFION film at -80C and am using a diamond knife
} to avoid flexing of the film. I have been trying to use the eyelash
} tool to pick up my section and transfer it to the grid. But in doing
} so my sections are rolling up and forming a tube instead of being
} flat. The problem I am having is transfering the cut sections on the
} diamond knife onto the TEM grid without destroying it. Any advice
} will be highly appreciated.
}
}
}
} Login Host: 160.36.234.57
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Tue Oct 13 09:09:28 2009
} 8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9DE9RQZ018510
} 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2009 09:09:28 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240801c6fa3a77e75e-at-[206.69.208.22]}
} 8, 11 -- Date: Tue, 13 Oct 2009 09:09:26 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: vkrishn3-at-utk.edu (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Cryomicrotoming hydrated NAFION film (178 microns thick)
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}





==============================Original Headers==============================
10, 24 -- From underwoo-at-u.washington.edu Wed Oct 14 14:54:47 2009
10, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134])
10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9EJslxh015580
10, 24 -- for {Microscopy-at-microscopy.Com} ; Wed, 14 Oct 2009 14:54:47 -0500
10, 24 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103])
10, 24 -- by mxout1.cac.washington.edu (8.14.3+UW09.06/8.14.3+UW09.05) with ESMTP id n9EJskgl006389
10, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
10, 24 -- Wed, 14 Oct 2009 12:54:46 -0700
10, 24 -- Received: from localhost (localhost [127.0.0.1])
10, 24 -- by hymn13.u.washington.edu (8.14.3+UW09.06/8.14.3+UW09.05) with ESMTP id n9EJsf3T004187;
10, 24 -- Wed, 14 Oct 2009 12:54:41 -0700
10, 24 -- X-Auth-Received: from [128.208.106.226] by hymn13.u.washington.edu via HTTP; Wed, 14 Oct 2009 12:54:41 PDT
10, 24 -- Date: Wed, 14 Oct 2009 12:54:41 -0700 (PDT)
10, 24 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
10, 24 -- To: vkrishn3-at-utk.edu, Microscopy List {Microscopy-at-microscopy.Com}
10, 24 -- Subject: Re: [Microscopy] viaWWW: Cryomicrotoming hydrated NAFION film (178
10, 24 -- microns thick)
10, 24 -- In-Reply-To: {200910131410.n9DEAkTY021274-at-ns.microscopy.com}
10, 24 -- Message-ID: {Pine.LNX.4.43.0910141254410.15983-at-hymn13.u.washington.edu}
10, 24 -- MIME-Version: 1.0
10, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
10, 24 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.14.194529
10, 24 -- X-Uwash-Spam: Gauge=X, Probability=10%, Report='
10, 24 -- TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __URI_NS '
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 14 Oct 2009 17:36:47 -0500
Subject: [Microscopy] Re: Replacement Pe gauge for DSM 962

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try here:

http://www.quintronix.com/Catalog_indexes/vacuum_gauges.html

and here:

http://www.highland-scientific.com/balzersbgd.html

and here;

http://valuvac.com/balzersbgd.html



gary g.


At 11:54 AM 10/14/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
15, 20 -- From gary-at-gaugler.com Wed Oct 14 17:36:47 2009
15, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9EMakSl005014
15, 20 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 17:36:47 -0500
15, 20 -- Message-Id: {200910142236.n9EMakSl005014-at-ns.microscopy.com}
15, 20 -- Received: (qmail 27885 invoked from network); 14 Oct 2009 16:11:07 -0700
15, 20 -- Received: by simscan 1.1.0 ppid: 27880, pid: 27882, t: 0.1056s
15, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
15, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
15, 20 -- by smtp1 with SMTP; 14 Oct 2009 16:11:07 -0700
15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
15, 20 -- Date: Wed, 14 Oct 2009 15:36:39 -0700
15, 20 -- To: tina-at-pbrc.hawaii.edu
15, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
15, 20 -- Subject: Re: [Microscopy] Replacement Pe gauge for DSM 962
15, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
15, 20 -- In-Reply-To: {200910141854.n9EIs8ro032720-at-ns.microscopy.com}
15, 20 -- References: {200910141854.n9EIs8ro032720-at-ns.microscopy.com}
15, 20 -- Mime-Version: 1.0
15, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: Adena.Rollins-at-sandisk.com
Date: Wed, 14 Oct 2009 19:18:06 -0500
Subject: [Microscopy] viaWWW: Pyramid Acoustic Tiling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Adena.Rollins-at-sandisk.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Adena.Rollins-at-sandisk.com
Name: Adena Rollins

Organization: SanDisk

Title-Subject: [Filtered] Pyramid Acoustic Tiling

Question: I am looking to do some long overdue "microscopy prepping"
of our lab. I would like to cover the walls with the pyramid
acoustic tiling and would like to know if anyone has a vendor that
they know of with a great deal right now. As with most
companies..money is tight now a days!

Thank you for any information,

Adena Rollins~

Login Host: 63.163.107.100
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Oct 14 19:18:05 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9F0I5Ud023812
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 19:18:05 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240805c6fc1aac75d5-at-[206.69.208.22]}
8, 11 -- Date: Wed, 14 Oct 2009 19:18:04 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: Adena.Rollins-at-sandisk.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Pyramid Acoustic Tiling
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: frah0010-at-umn.edu
Date: Wed, 14 Oct 2009 19:57:22 -0500
Subject: [Microscopy] Re: viaWWW: Pyramid Acoustic Tiling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Adena,

I'm not sure who has the best deal now -- you'll probably have to ask
for quotes from vendors.

I can say, though, that I installed such panels in the Electron
Microprobe Lab several years ago -- you can see them on the walls
here...

http://probelab.geo.umn.edu/facilities.html

... and I have been very pleased with the acoustic difference they've
made.

Best,
Ellery

-----------------
Ellery Frahm
Manager & Principal Analyst, Electron Microprobe Lab
Senior Research Fellow, Department of Geology & Geophysics
University of Minnesota - Twin Cities
http://probelab.geo.umn.edu



On Oct 14, 2009, at 7:22 PM, Adena.Rollins-at-sandisk.com wrote:

} Email: Adena.Rollins-at-sandisk.com
} Name: Adena Rollins
}
} Organization: SanDisk
}
} Title-Subject: [Filtered] Pyramid Acoustic Tiling
}
} Question: I am looking to do some long overdue "microscopy prepping"
} of our lab. I would like to cover the walls with the pyramid
} acoustic tiling and would like to know if anyone has a vendor that
} they know of with a great deal right now. As with most
} companies..money is tight now a days!
}
} Thank you for any information,
}
} Adena Rollins

==============================Original Headers==============================
11, 20 -- From frah0010-at-umn.edu Wed Oct 14 19:57:22 2009
11, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9F0vMY4007296
11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 19:57:22 -0500
11, 20 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206])
11, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP
11, 20 -- Wed, 14 Oct 2009 19:57:21 -0500 (CDT)
11, 20 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN
11, 20 -- Cc: Adena.Rollins-at-sandisk.com
11, 20 -- Message-Id: {8ABF12AE-3409-4CB3-9E1A-0DD2DA0D10FE-at-umn.edu}
11, 20 -- From: Ellery Frahm {frah0010-at-umn.edu}
11, 20 -- To: microscopy-at-microscopy.com
11, 20 -- In-Reply-To: {200910150022.n9F0MheE031332-at-ns.microscopy.com}
11, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
11, 20 -- Content-Transfer-Encoding: 7bit
11, 20 -- Mime-Version: 1.0 (Apple Message framework v936)
11, 20 -- Subject: Re: [Microscopy] viaWWW: Pyramid Acoustic Tiling
11, 20 -- Date: Wed, 14 Oct 2009 19:57:20 -0500
11, 20 -- References: {200910150022.n9F0MheE031332-at-ns.microscopy.com}
11, 20 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: doc.vrdoljak-at-gmail.com
Date: Thu, 15 Oct 2009 15:04:26 -0500
Subject: [Microscopy] question about resins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I was looking for a good water soluble resin to use for embedding some
materials and doing course sectioning for light microscopy. I
remember using nanoplast in the past, but I can't see it being sold
anymore.

My ideal resin would be something that is water soluble, low
viscosity, and harden rapidly for hand cutting to a block for large
sections on a light microscope. The material we will be looking at is
very similar to coal but with pore structure and weak areas that need
to reinforced and filled in with a plastic resin.

Can anyone also recommend a good fluorescent pigment to use for
porosity mapping in a dark material described above?
Thanks.

==============================Original Headers==============================
3, 29 -- From doc.vrdoljak-at-gmail.com Thu Oct 15 15:04:25 2009
3, 29 -- Received: from mail-iw0-f187.google.com (mail-iw0-f187.google.com [209.85.223.187])
3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9FK4Puj004475
3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 15:04:25 -0500
3, 29 -- Received: by iwn17 with SMTP id 17so717814iwn.10
3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 13:04:25 -0700 (PDT)
3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 29 -- d=gmail.com; s=gamma;
3, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
3, 29 -- :from:to:content-type;
3, 29 -- bh=evZYOOtq5zR/VOy0RE01LAhGAAJE7wgeuXFtXjvGi0U=;
3, 29 -- b=SOkYLDLJhqNODFY2aSSfq3zWe3tDifOFHdyG38zHL8251Wn7t0g9j7YUuNOGbLgKGG
3, 29 -- CAKFlEAQuZ/GUXo0UCYbWHD4fgbAhu7dolisbzRAIOPTwRxNzy74HVWabIfZ9RoVViVj
3, 29 -- FVWylyzlIZZiE2lqiaFFE+c+LXgMv3yKQhVMY=
3, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
3, 29 -- d=gmail.com; s=gamma;
3, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
3, 29 -- b=FTrcA9L320t8bMAZ8F/4R1GTYFMV+TRz/uVQOVc2JWQxQS1TlD8zv5ebGX3Ur40veB
3, 29 -- /HVwYeRUuQOKGrLKoj+oRNhv902kNmA6MfY1iNH++faaFSeu1fC+BQF6mIe6buN2cHRs
3, 29 -- yhVIiKD10ClicaR5Cbes5UPHQT1oV25CXDKgM=
3, 29 -- MIME-Version: 1.0
3, 29 -- Received: by 10.231.25.29 with SMTP id x29mr1443228ibb.31.1255637065416; Thu,
3, 29 -- 15 Oct 2009 13:04:25 -0700 (PDT)
3, 29 -- Date: Thu, 15 Oct 2009 13:04:25 -0700
3, 29 -- Message-ID: {fb272960910151304n1c4c2e60y5b205376bdcc5b71-at-mail.gmail.com}
3, 29 -- Subject: question about resins
3, 29 -- From: Gordon Vrdoljak {doc.vrdoljak-at-gmail.com}
3, 29 -- To: microscopy-at-microscopy.com
3, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Thu, 15 Oct 2009 15:40:20 -0500
Subject: [Microscopy] Re: question about resins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds like JB-4. Available from whomever is your favourite supplier.
characteristics, from what I remember are that it's Glycol Methacrylate
based and water soluble. The catalyzed monomer is used as a dehydration
and infiltration agent, and you can substitute in before 100% EtOH
dehydration if you want - didn’t on the few occasions I used it. Less
shrinkage than with paraffin, but you probably don’t want parafin
anyway. Gives good sectioning down to about 1-2?, at least for me, and
has relatively good stability in the beam if you really want to look at
something that thick, which you may wish to do with your coal samples.
Also, can take relatively large samples.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
5, 21 -- From paul_hazelton-at-umanitoba.ca Thu Oct 15 15:40:19 2009
5, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9FKeJDS019975
5, 21 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 15:40:19 -0500
5, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
5, 21 -- (authenticated bits=0)
5, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n9FKeIlg000674;
5, 21 -- Thu, 15 Oct 2009 15:40:18 -0500 (CDT)
5, 21 -- Message-ID: {4AD788B8.80809-at-umanitoba.ca}
5, 21 -- Date: Thu, 15 Oct 2009 15:40:24 -0500
5, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 21 -- MIME-Version: 1.0
5, 21 -- To: doc.vrdoljak-at-gmail.com
5, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
5, 21 -- Subject: Re: [Microscopy] question about resins
5, 21 -- References: {200910152006.n9FK6cMv007190-at-ns.microscopy.com}
5, 21 -- In-Reply-To: {200910152006.n9FK6cMv007190-at-ns.microscopy.com}
5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 21 -- Content-Transfer-Encoding: 8bit
5, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: swalck-at-southbaytech.com
Date: Thu, 15 Oct 2009 15:50:38 -0500
Subject: [Microscopy] viaWWW: Crystalbond

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our Quickstick 135 is a similar composition to the Crystalbond. When we did
the work on the plasma cleaner, we used it as an intentional contaminant.
See the following paper for the details:

Surface Science Aspects of Contamination in TEM Sample Preparation, J. T.
Grant, S. D. Walck, F. J. Scheltens, A. A. Voevodin, Proceedings of the
Materials Research Society, Workshop on Specimen Preparation for
Transmission Electron Microscopy of Materials IV, eds. Ron M. Anderson and
Scott D. Walck, Pittsburgh, Vol 480, (1997).

When we tested the samples in the TEM, you could have the balls of the
materials just next to the beam and you would not get any excessive increase
in contamination on the sample. It wasn't until you intentionally put the
beam on the wax did it boil off an evaporate into the vacuum.

I have used it to bond samples in the SEM without problems. As long as the
sample does not get hot or the beam hits the material directly, it doesn't
seem to be an issue using it. I prefer not to use it, but if I want to
polish the sample after examining it first, I'll use it.

-Scott
 
Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA  92673
 
US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499
 
www.southbaytech.com
swalck-at-southbaytech.com


-----Original Message-----
X-from: marcello.serracino-at-igag.cnr.it [mailto:marcello.serracino-at-igag.cnr.it]

Sent: Wednesday, October 14, 2009 5:47 AM
To: swalck-at-southbaytech.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both marcello.serracino-at-igag.cnr.it as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: marcello.serracino-at-igag.cnr.it
Name: Marcello Serracino

Organization: National Research Council CNR-Italy

Title-Subject: [Filtered] Crystalbond

Question: Does anyone know if crystalbond embedding samples can
affect or contaminate the microprobe?
I would appreciate any suggestion.
Best Regards
Marcello Serracino

Login Host: 151.100.51.18
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Oct 14 07:37:49 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9ECbmXZ031411
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2009 07:37:48
-0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c6fb768bfd4f-at-[206.69.208.22]}
6, 11 -- Date: Wed, 14 Oct 2009 07:37:47 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: marcello.serracino-at-igag.cnr.it (by way of
MicroscopyListserver)
6, 11 -- Subject: viaWWW: Crystalbond
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




==============================Original Headers==============================
21, 24 -- From swalck-at-southbaytech.com Thu Oct 15 15:50:38 2009
21, 24 -- Received: from relay00.pair.com (relay00.pair.com [209.68.5.9])
21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9FKob7e002197
21, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 15:50:38 -0500
21, 24 -- Received: (qmail 45202 invoked from network); 15 Oct 2009 20:50:36 -0000
21, 24 -- Received: from 99.154.21.201 (HELO SWALCKD1) (99.154.21.201)
21, 24 -- by relay00.pair.com with SMTP; 15 Oct 2009 20:50:36 -0000
21, 24 -- X-pair-Authenticated: 99.154.21.201
21, 24 -- From: "Scott Walck" {swalck-at-southbaytech.com}
21, 24 -- To: {Microscopy-at-microscopy.com}
21, 24 -- Cc: {marcello.serracino-at-igag.cnr.it}
21, 24 -- References: {200910141247.n9ECl0Wr012390-at-ns.microscopy.com}
21, 24 -- In-Reply-To: {200910141247.n9ECl0Wr012390-at-ns.microscopy.com}
21, 24 -- Subject: RE: [Microscopy] viaWWW: Crystalbond
21, 24 -- Date: Thu, 15 Oct 2009 13:50:26 -0700
21, 24 -- Message-ID: {005a01ca4dd9$1feb5980$5fc20c80$-at-com}
21, 24 -- MIME-Version: 1.0
21, 24 -- Content-Type: text/plain;
21, 24 -- charset="iso-8859-1"
21, 24 -- X-Mailer: Microsoft Office Outlook 12.0
21, 24 -- thread-index: AcpMzGxzSApiup2ySdekNHfmEZmFAgAVxMNg
21, 24 -- Content-Language: en-us
21, 24 -- Content-Transfer-Encoding: 8bit
21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9FKob7e002197
==============================End of - Headers==============================




From: y.han-at-shef.ac.uk
Date: Thu, 15 Oct 2009 16:33:31 -0500
Subject: [Microscopy] viaWWW: CCD camera within a TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both y.han-at-shef.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: y.han-at-shef.ac.uk
Name: Yisong Han

Organization: University of Sheffield

Title-Subject: [Filtered] CCD camera within a TEM

Question: Hi,

We got a Tietz (also TVIPS) FastScan-F114T CCD within our Jeol 3010
TEM. May I ask if this camera is suited for observing and recording
diffraction patterns? There are very limited information about this.
I have to ask for advice to avoid any permanent damage to the
scintillator. Thanks very much in advance.

Yisong

Login Host: 143.167.182.55
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Oct 15 16:33:30 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9FLXUkA017965
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 16:33:30 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6fd45978656-at-[206.69.208.22]}
8, 11 -- Date: Thu, 15 Oct 2009 16:33:30 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: y.han-at-shef.ac.uk (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: CCD camera within a TEM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bnross-at-interchange.ubc.ca
Date: Thu, 15 Oct 2009 17:23:35 -0500
Subject: [Microscopy] Balzers MED 010 Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

We have been having a problem with our Balzers MED 010; it won't switch on the high voltage to allow carbon evaporation, glow discharging, etc. The problem was intermittent at first, and could be remedied with a good solid smack on the top of the machine, but now the "HT Free" light and thus the evaporating/sputtering/glow discharging controls remain off thereby rendering the unit quite useless. I checked all the fuses and safety interlock switches with a multimeter and they are all functioning properly. I checked the manual and schematic and discovered there are two "vacuum relays" but no mention of how they determine when the vacuum is sufficiently high to allow the HT to be switched on. I can manually switch on the relays, which turns on the HT Free light, but not the actual HT, as the controls still remain off. I'm guessing there is something wrong with the sensor/gauge that tells the relays that it's ok to switch on the HT. The ultimate vacuum in the chamber seems to be fine at around 2x10^-5 - 6x10^-6 mbar as indicated by the main high vacuum gauge. During normal operation in the past, the HT Free light would switch on at around 6x10^-1 mbar.

Has anyone had this problem with an MED 010 before? Any helpful hints, tips, or tricks would be much appreciated!

Thanks,
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-6996



==============================Original Headers==============================
8, 28 -- From bnross-at-interchange.ubc.ca Thu Oct 15 17:23:35 2009
8, 28 -- Received: from mr2.mail-relay.ubc.ca (mr2.mail-relay.ubc.ca [137.82.45.3])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9FMNZbh001448
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 17:23:35 -0500
8, 28 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
8, 28 -- by mr2.mail-relay.ubc.ca (Postfix) with ESMTP id 5D8221857D
8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 15:23:34 -0700 (PDT)
8, 28 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12])
8, 28 -- by smtp.interchange.ubc.ca
8, 28 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
8, 28 -- with ESMTP id {0KRK00HICU790V-at-smtp.interchange.ubc.ca} for
8, 28 -- Microscopy-at-microscopy.com; Thu, 15 Oct 2009 15:23:34 -0700 (PDT)
8, 28 -- Date: Thu, 15 Oct 2009 15:23:33 -0700 (PDT)
8, 28 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
8, 28 -- Subject: Balzers MED 010 Trouble
8, 28 -- To: Microscopy-at-microscopy.com
8, 28 -- Message-id: {33514548.36931255645413245.JavaMail.myubc2-at-brahms.my.ubc.ca}
8, 28 -- MIME-version: 1.0
8, 28 -- X-Mailer: uPortal WEB email client 3.0
8, 28 -- Content-type: text/plain; charset=us-ascii
8, 28 -- X-UBC-Scanned: Sophos PureMessage 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.10.15.220928
8, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
8, 28 -- X-PerlMx-Spam: Probability=8%, Report=
8, 28 -- SUPERLONG_LINE 0.05, BODY_SIZE_1500_1599 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0
8, 28 -- X-Spam-Level:
8, 28 -- X-Spam-Flag: No
8, 28 -- Content-Transfer-Encoding: 8bit
8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9FMNZbh001448
==============================End of - Headers==============================




From: Woody.White-at-areva.com
Date: Fri, 16 Oct 2009 08:21:24 -0500
Subject: [Microscopy] Cleaning aluminum parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Usually the dark finish resulting from alkaline cleaning will leave
"smut" on the surface that comes from the alloy ingredients (typ Mg, Cu,
Fe). A quick dip in fairly concentrated HNO3, followed by a rinse will
usually dissolve the smut.

Woody

N.W. (Woody) White Jr.
Electron Microscopist
Chemistry and Materials Center
AREVA NP Inc
An AREVA and Siemens Company
Lynchburg, VA
Lab Phone: 434.832.3004

-----Original Message-----
X-from: kenconverse-at-qualityimages.biz
[mailto:kenconverse-at-qualityimages.biz]
Sent: Monday, October 12, 2009 9:16 AM
To: WHITE Norvell (AREVA NP INC)

Debbie,
I believe a dip in nitric acid will clean them up. Maybe Woody White
can chime in here about concentration and length of time. He originally
told me about this.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Friday, October 09, 2009 9:39 AM
To: kenconverse-at-qualityimages.biz

Unfortunately some aluminum planchettes used in high pressure freezing
were
left in water containing detergent for a few hours before cleaning was
completed. As a result the planchettes turned dark in color.

It is much more difficult to work with them in this condition. Does
anyone
know a way to remove the tarnish and make them nice and shiny again?

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy





==============================Original
Headers==============================
9, 31 -- From dsherman-at-purdue.edu Fri Oct 9 08:36:45 2009
9, 31 -- Received: from mailhub130.itcs.purdue.edu
(mailhub130.itcs.purdue.edu [128.210.5.130])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id
n99DajH5002451
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009
08:36:45
-0500
9, 31 -- Received: from mailhub126.itcs.purdue.edu
(mailhub126.itcs.purdue.edu [128.210.5.126])
9, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx)
with ESMTP id n99DajKA007535
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009
09:36:45
-0400
9, 31 -- Received: from WPPEXHUB03H.purdue.lcl
(wppexhub03h.itap.purdue.edu
[172.21.6.92])
9, 31 -- by mailhub126.itcs.purdue.edu
(8.14.2/8.14.2/exchange-outbound) with ESMTP id n99DajKp012208
9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Oct 2009
09:36:45
-0400
9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.16]) by
WPPEXHUB03H.purdue.lcl
9, 31 -- ([172.21.6.92]) with mapi; Fri, 9 Oct 2009 09:36:44 -0400
9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
9, 31 -- Date: Fri, 9 Oct 2009 09:36:41 -0400
9, 31 -- Subject: Cleaning aluminum parts
9, 31 -- Thread-Topic: Cleaning aluminum parts
9, 31 -- Thread-Index: AcpI5YiahaNevBxLQ1qYopsj7xAsxA==
9, 31 -- Message-ID: {C6F4B4A9.13DB%dsherman-at-purdue.edu}
9, 31 -- Accept-Language: en-US
9, 31 -- Content-Language: en-US
9, 31 -- X-MS-Has-Attach:
9, 31 -- X-MS-TNEF-Correlator:
9, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
9, 31 -- acceptlanguage: en-US
9, 31 -- Content-Type: text/plain; charset="us-ascii"
9, 31 -- MIME-Version: 1.0
9, 31 -- X-PMX-Version: 5.5.7.378829
9, 31 -- X-PerlMx-Virus-Scanned: Yes
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n99DajH5002451
==============================End of -
Headers==============================




==============================Original
Headers==============================
21, 25 -- From kenconverse-at-qualityimages.biz Mon Oct 12 08:06:31 2009
21, 25 -- Received: from cdptpa-omtalb.mail.rr.com
(cdptpa-omtalb.mail.rr.com [75.180.132.122])
21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id n9CD6TtP008762
21, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2009
08:06:31 -0500
21, 25 -- Received: from Ken ([72.227.111.133]) by
cdptpa-omta01.mail.rr.com
21, 25 -- with ESMTP
21, 25 -- id
{20091012130627700.XRWO16663-at-cdptpa-omta01.mail.rr.com} ;
21, 25 -- Mon, 12 Oct 2009 13:06:27 +0000
21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 25 -- To: {dsherman-at-purdue.edu} , "MSA Listserver"
{microscopy-at-microscopy.com}
21, 25 -- Subject: RE: [Microscopy] Cleaning aluminum parts
21, 25 -- Date: Mon, 12 Oct 2009 09:06:19 -0400
21, 25 -- Message-ID: {9AB76387B3704F05BC8870475EAC8253-at-Ken}
21, 25 -- MIME-Version: 1.0
21, 25 -- Content-Type: text/plain;
21, 25 -- charset="US-ASCII"
21, 25 -- X-Priority: 3 (Normal)
21, 25 -- X-MSMail-Priority: Normal
21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 25 -- Importance: Normal
21, 25 -- Thread-Index: AcpI5epa5v54l5kqS8KFrAJmStRRFQCVpI8g
21, 25 -- In-Reply-To: {200910091339.n99DdPJQ006353-at-ns.microscopy.com}
21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 25 -- Content-Transfer-Encoding: 8bit
21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9CD6TtP008762
==============================End of -
Headers==============================


==============================Original Headers==============================
30, 30 -- From Woody.White-at-areva.com Fri Oct 16 08:21:22 2009
30, 30 -- Received: from smtpout73.framatome-anp.us (smtpout73.framatome-anp.us [160.84.253.73])
30, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GDLMkW019036
30, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 08:21:22 -0500
30, 30 -- Received: from RUSAHUBBX03.ad.corp (unknown [10.2.161.112])
30, 30 -- by smtpout73.framatome-anp.us (Postfix) with ESMTP id 1119A26C524
30, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 13:21:22 +0000 (UTC)
30, 30 -- Received: from auslyncbx02.adom.ad.corp ([160.84.192.44]) by RUSAHUBBX03.ad.corp with Microsoft SMTPSVC(6.0.3790.3959);
30, 30 -- Fri, 16 Oct 2009 09:21:22 -0400
30, 30 -- Received: from AUSLYNCMX02.adom.ad.corp ([160.84.193.182]) by auslyncbx02.adom.ad.corp with Microsoft SMTPSVC(6.0.3790.3959);
30, 30 -- Fri, 16 Oct 2009 09:21:21 -0400
30, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
30, 30 -- Content-class: urn:content-classes:message
30, 30 -- MIME-Version: 1.0
30, 30 -- Content-Type: text/plain;
30, 30 -- charset="us-ascii"
30, 30 -- Subject: RE: [Microscopy] RE: Cleaning aluminum parts
30, 30 -- Date: Fri, 16 Oct 2009 09:21:21 -0400
30, 30 -- Message-ID: {BC417D9255991046A37DD56CF597DB71042123BC-at-AUSLYNCMX02.adom.ad.corp}
30, 30 -- In-Reply-To: {200910121316.n9CDGHLf022255-at-ns.microscopy.com}
30, 30 -- X-MS-Has-Attach:
30, 30 -- X-MS-TNEF-Correlator:
30, 30 -- Thread-Topic: [Microscopy] RE: Cleaning aluminum parts
30, 30 -- Thread-Index: AcpLPjBGL6D6YmJSSb6l02/5ZRgdWADJLcHA
30, 30 -- References: {200910121316.n9CDGHLf022255-at-ns.microscopy.com}
30, 30 -- From: "WHITE Norvell (AREVA NP INC)" {Woody.White-at-areva.com}
30, 30 -- To: {Microscopy-at-microscopy.com}
30, 30 -- X-OriginalArrivalTime: 16 Oct 2009 13:21:21.0940 (UTC) FILETIME=[8DB10D40:01CA4E63]
30, 30 -- Content-Transfer-Encoding: 8bit
30, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9GDLMkW019036
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Fri, 16 Oct 2009 09:10:44 -0500
Subject: [Microscopy] viaWWW: Glow Dischargers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David

Organization: SAIC

Title-Subject: [Filtered] Glow Dischargers

Question: Hello,

I'd like to know if anyone has recommendations for tabletop
glow-discharge units? Thanks!

- David

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Oct 16 09:10:44 2009
8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GEAf2b003328
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 09:10:43 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6fe2f514f0b-at-[206.69.208.22]}
8, 11 -- Date: Fri, 16 Oct 2009 09:10:40 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Glow Dischargers
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Chris.Guerin-at-dmbr.vib-UGent.be
Date: Fri, 16 Oct 2009 09:43:14 -0500
Subject: [Microscopy] TEM-vintage equipment servicing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone:

I've recently acquired an elderly but still working LKB III Ultratome
and knife maker. I would like to have them serviced but don't know of
anyone in Europe who still services LKB equipment. Does anyone know of
someone who does?

Many thanks,

Chris Guerin
Leader, Microscopy Core
VIB-Dept. of Molecular Biomedical Research
Ghent, Belgium

==============================Original Headers==============================
4, 31 -- From Chris.Guerin-at-dmbr.vib-UGent.be Fri Oct 16 09:43:14 2009
4, 31 -- Received: from smtp2.UGent.be (smtp2.ugent.be [157.193.49.126])
4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GEhEsN019346
4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 Oct 2009 09:43:14 -0500
4, 31 -- Received: from localhost (mcheck3.ugent.be [157.193.71.89])
4, 31 -- by smtp2.UGent.be (Postfix) with ESMTP id DF5B444A244
4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 Oct 2009 16:43:13 +0200 (CEST)
4, 31 -- X-Virus-Scanned: by UGent DICT
4, 31 -- Received: from smtp2.UGent.be ([157.193.49.126])
4, 31 -- by localhost (mcheck3.ugent.be [157.193.43.11]) (amavisd-new, port 10024)
4, 31 -- with ESMTP id B-z6ToiMSioh for {Microscopy-at-Microscopy.Com} ;
4, 31 -- Fri, 16 Oct 2009 16:43:12 +0200 (CEST)
4, 31 -- Received: from smtp.dmbr.UGent.be (dmbr242.fvms.ugent.be [157.193.189.5])
4, 31 -- by smtp2.UGent.be (Postfix) with ESMTP id 0E24B44A23C
4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 Oct 2009 16:43:11 +0200 (CEST)
4, 31 -- Received: from dmbr129.fvms.ugent.be (dmbr129.fvms.ugent.be [157.193.200.116])
4, 31 -- by smtp.dmbr.UGent.be (Postfix) with ESMTP id C5AC680115
4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 Oct 2009 16:43:11 +0200 (CEST)
4, 31 -- From: Chris Guerin {Chris.Guerin-at-dmbr.vib-UGent.be}
4, 31 -- Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
4, 31 -- Content-Transfer-Encoding: 7bit
4, 31 -- Subject: TEM-vintage equipment servicing
4, 31 -- Date: Fri, 16 Oct 2009 16:35:45 +0200
4, 31 -- Message-Id: {094E35F2-287B-4DE1-8744-9FEE45C7469F-at-dmbr.vib-UGent.be}
4, 31 -- To: Microscopy-at-Microscopy.Com
4, 31 -- Mime-Version: 1.0 (Apple Message framework v1076)
4, 31 -- X-Mailer: Apple Mail (2.1076)
4, 31 -- X-Miltered: at mcheck2 with ID 4AD8867F.007 by Joe's j-chkmail (http://helpdesk.ugent.be/email/)!
4, 31 -- X-j-chkmail-Enveloppe: 4AD8867F.007/157.193.189.5/dmbr242.fvms.ugent.be/smtp.dmbr.UGent.be/ {Chris.Guerin-at-dmbr.vib-UGent.be}
4, 31 -- X-j-chkmail-Score: MSGID : 4AD8867F.007 on smtp2.UGent.be : j-chkmail score : . : R=. U=. O=. B=0.000 -} S=0.000
4, 31 -- X-j-chkmail-Status: Ham
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Fri, 16 Oct 2009 13:35:16 -0500
Subject: [Microscopy] Re: viaWWW: Glow Dischargers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi David,

We use a Harrick PDC-32G http://www.harrickplasma.com/products_cleaners.php
for basic treatment of carbon films to make them wettable. It has been
extremely reliable and easy to use; takes 2 min to treat grids (pumpdown
+ 30s treatment on low setting). (No connection with Harrick.....)

There are other ways to go if you like the cheap and DIY route. Tom
Pollard patented a very simple device using a handheld Tesla coil
installed into a plastic dessicator. You can find this via Google. I
have a copy of that if it is useful to you. I think he patented it to
keep it in the public domain - it is too simple to think he meant to
profit from it. Thank you Tom!

Dale





parmiterd-at-mail.nih.gov wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: parmiterd-at-mail.nih.gov
} Name: David
}
} Organization: SAIC
}
} Title-Subject: [Filtered] Glow Dischargers
}
} Question: Hello,
}
} I'd like to know if anyone has recommendations for tabletop
} glow-discharge units? Thanks!
}
} - David
}
} Login Host: 129.43.43.217
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Fri Oct 16 09:10:44 2009
} 8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GEAf2b003328
} 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 09:10:43 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c6fe2f514f0b-at-[206.69.208.22]}
} 8, 11 -- Date: Fri, 16 Oct 2009 09:10:40 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Glow Dischargers
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================

==============================Original Headers==============================
9, 22 -- From dac-at-research.umass.edu Fri Oct 16 13:35:16 2009
9, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GIZG7j014571
9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 13:35:16 -0500
9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
9, 22 -- (authenticated bits=0)
9, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n9GIZEcO028240
9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
9, 22 -- Fri, 16 Oct 2009 14:35:15 -0400
9, 22 -- Message-ID: {4AD8BCF0.4020707-at-research.umass.edu}
9, 22 -- Date: Fri, 16 Oct 2009 14:35:28 -0400
9, 22 -- From: Dale Callaham {dac-at-research.umass.edu}
9, 22 -- Reply-To: dac-at-research.umass.edu
9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.23) Gecko/20090825 SeaMonkey/1.1.18
9, 22 -- MIME-Version: 1.0
9, 22 -- To: parmiterd-at-mail.nih.gov, Microscopy Listserver {Microscopy-at-microscopy.com}
9, 22 -- Subject: Re: [Microscopy] viaWWW: Glow Dischargers
9, 22 -- References: {200910161414.n9GEEmtf011188-at-ns.microscopy.com}
9, 22 -- In-Reply-To: {200910161414.n9GEEmtf011188-at-ns.microscopy.com}
9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: yanga-at-agr.gc.ca
Date: Fri, 16 Oct 2009 18:28:45 -0500
Subject: [Microscopy] viaWWW: suppliers for high pressure freezing system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yanga-at-agr.gc.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yanga-at-agr.gc.ca
Name: Ann Fook Yang

Organization: Agriculture and Agri-Food Canada

Title-Subject: [Filtered] suppliers for high pressure freezing system

Question: Hi,

Do you know of suppliers for high pressure freezing system other than
Leica microsystem? Thank.

Ann Fook

Login Host: 192.197.71.189
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Oct 16 18:28:45 2009
8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GNSgbU007115
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 18:28:43 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c6feb217f610-at-[206.69.208.22]}
8, 11 -- Date: Fri, 16 Oct 2009 18:28:40 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: yanga-at-agr.gc.ca (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: suppliers for high pressure freezing system
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dyel-at-mail.nih.gov
Date: Fri, 16 Oct 2009 18:29:11 -0500
Subject: [Microscopy] viaWWW: Negative Staining for TEM Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dyel-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dyel-at-mail.nih.gov
Name: Chip

Organization: NIH-NICHD

Title-Subject: [Filtered] Negative Staining for TEM Imaging

Question: Hello,

Is anyone familiar with the ìdouble sandwich
technique" for EM imaging of ìparticlesî? (i.e.
pre-ribosomal bodies)

What is the advantage of this technique over "standard" negative staining?

Thank you in advance!

Chip






Login Host: 128.231.223.174
---------------------------------------------------------------------------


==============================Original Headers==============================
16, 13 -- From zaluzec-at-microscopy.com Fri Oct 16 18:29:11 2009
16, 13 -- Received: from [206.69.208.22] ([130.202.238.72])
16, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GNT9Ud007460
16, 13 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 18:29:10 -0500
16, 13 -- Mime-Version: 1.0
16, 13 -- Message-Id: {p06240801c6feb231fc22-at-[206.69.208.22]}
16, 13 -- Date: Fri, 16 Oct 2009 18:29:07 -0500
16, 13 -- To: microscopy-at-microscopy.com
16, 13 -- From: dyel-at-mail.nih.gov (by way of MicroscopyListserver)
16, 13 -- Subject: viaWWW: Negative Staining for TEM Imaging
16, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
16, 13 -- Content-Transfer-Encoding: 8bit
16, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9GNT9Ud007460
==============================End of - Headers==============================




From: jshields-at-cb.uga.edu
Date: Fri, 16 Oct 2009 18:29:49 -0500
Subject: [Microscopy] viaWWW: loss of vacuum bringing up FE tip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jshields-at-cb.uga.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jshields-at-cb.uga.edu
Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] loss of vacuum bringing up FE tip

Question: We are having trouble with an older FE-SEM. LEO (Zeiss)982.
After a shut down over the weekend from power failure we got the
vacuum system to pump down (initial problem with ion-getter). The
tip warms up fine and we get images, but after about 30 minutes the
gun chamber vacuum suddenly shuts off and then immediately re-starts
showing vacuum is good. So we can't keep the gun on longer than 30
minutes. Anyone know what's wrong and a possible solution? Maybe
even someone to contact who would know?
Thanks, Please respond off-listserve at:
jshields-at-cb.uga.edu

John Shields

Login Host: 128.192.63.15
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Fri Oct 16 18:29:49 2009
7, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GNTmna008833
7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 18:29:49 -0500
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c6feb24e02ce-at-[206.69.208.22]}
7, 11 -- Date: Fri, 16 Oct 2009 18:29:47 -0500
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: jshields-at-cb.uga.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: loss of vacuum bringing up FE tip
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Sat, 17 Oct 2009 00:52:26 -0500
Subject: [Microscopy] Re: viaWWW: suppliers for high pressure freezing system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes, RMC-Boeckeler produces the high pressure freezer formerly sold by
Bal-Tec. See http://www.rmcproducts.com/
...Just a satisfied customer.

Best regards,
Stefan



yanga-at-agr.gc.ca schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both yanga-at-agr.gc.ca as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: yanga-at-agr.gc.ca
} Name: Ann Fook Yang
}
} Organization: Agriculture and Agri-Food Canada
}
} Title-Subject: [Filtered] suppliers for high pressure freezing system
}
} Question: Hi,
}
} Do you know of suppliers for high pressure freezing system other than
} Leica microsystem? Thank.
}
} Ann Fook
}
} Login Host: 192.197.71.189
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Fri Oct 16 18:28:45 2009
} 8, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
} 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9GNSgbU007115
} 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2009 18:28:43 -0500
} 8, 11 -- Mime-Version: 1.0
} 8, 11 -- Message-Id: {p06240800c6feb217f610-at-[206.69.208.22]}
} 8, 11 -- Date: Fri, 16 Oct 2009 18:28:40 -0500
} 8, 11 -- To: microscopy-at-microscopy.com
} 8, 11 -- From: yanga-at-agr.gc.ca (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: suppliers for high pressure freezing system
} 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Sat Oct 17 00:52:26 2009
7, 22 -- Received: from mailout09.t-online.de (mailout09.t-online.de [194.25.134.84])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9H5qPnR002796
7, 22 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 00:52:25 -0500
7, 22 -- Received: from fwd04.aul.t-online.de
7, 22 -- by mailout09.t-online.de with smtp
7, 22 -- id 1Mz2DI-0007NA-02; Sat, 17 Oct 2009 07:52:24 +0200
7, 22 -- Received: from [192.168.2.101] (Gz+nLYZX8htew14yTRorOZUwnuEN4v+-HmVqE-YpKh4h4tigTY1NOLy2q3wa5TTwIZ-at-[93.222.114.39]) by fwd04.aul.t-online.de
7, 22 -- with esmtp id 1Mz2DG-0d42m80; Sat, 17 Oct 2009 07:52:22 +0200
7, 22 -- Message-ID: {4AD95B96.2060203-at-t-online.de}
7, 22 -- Date: Sat, 17 Oct 2009 07:52:22 +0200
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: [Microscopy] viaWWW: suppliers for high pressure freezing system
7, 22 -- References: {200910162336.n9GNaCHw027140-at-ns.microscopy.com}
7, 22 -- In-Reply-To: {200910162336.n9GNaCHw027140-at-ns.microscopy.com}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID: Gz+nLYZX8htew14yTRorOZUwnuEN4v+-HmVqE-YpKh4h4tigTY1NOLy2q3wa5TTwIZ
7, 22 -- X-TOI-MSGID: 2a04bd1a-8ec6-4687-8a37-61de4390b39b
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Sat, 17 Oct 2009 10:42:48 -0500
Subject: [Microscopy] high pressure freezing system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} } }
} Title-Subject: [Filtered] suppliers for high pressure freezing system
}
} Question: Hi,
}
} Do you know of suppliers for high pressure freezing system other than
} Leica microsystem? Thank.

one other supplier is Martin Wohlwend in Switzerland.
Engineering Office M. Wohlwend
Bifig 14, CH-9466 Sennwald, Switzerland
Tel +41 81 7571924
Fax +41 81 7572243

and: RMC

best regards,
Reinhard


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
9, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Sat Oct 17 10:42:47 2009
9, 25 -- Received: from rrzmta5.rz.uni-regensburg.de (rrzmta5.rz.uni-regensburg.de [194.94.155.56])
9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9HFgjAW017053
9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 10:42:47 -0500
9, 25 -- Received: from rrzmta5.rz.uni-regensburg.de (localhost [127.0.0.1])
9, 25 -- by localhost (Postfix) with SMTP id 4CD36B9B
9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 17:42:43 +0200 (CEST)
9, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
9, 25 -- by rrzmta5.rz.uni-regensburg.de (Postfix) with ESMTP id 295A8B7D
9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 17:42:43 +0200 (CEST)
9, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
9, 25 -- with Novell_GroupWise; Sat, 17 Oct 2009 17:42:43 +0200
9, 25 -- Message-Id: {4ADA0210020000540000A876-at-gwsmtp1.uni-regensburg.de}
9, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.1
9, 25 -- Date: Sat, 17 Oct 2009 17:42:40 +0200
9, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
9, 25 -- To: {yanga-at-agr.gc.ca} , {microscopy-at-microscopy.com}
9, 25 -- Subject: high pressure freezing system
9, 25 -- References: {200910162329.n9GNT7cX007457-at-ns.microscopy.com}
9, 25 -- In-Reply-To: {200910162329.n9GNT7cX007457-at-ns.microscopy.com}
9, 25 -- Mime-Version: 1.0
9, 25 -- Content-Type: text/plain; charset=US-ASCII
9, 25 -- Content-Disposition: inline
9, 25 -- Content-Transfer-Encoding: 8bit
9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9HFgjAW017053
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Sat, 17 Oct 2009 16:14:35 -0500
Subject: [Microscopy] Re: high pressure freezing system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Wohlwend High Pressure freezer is marketed in the USA through:

TechnoTrade International (ATTN: Johnny Hagen)
7 Perimeter Road
Manchester, NH 03103-3343
Phone: 800-875-3713
FAX: 603-622-5211
Sales-at-technotradeinc.com

Another satisfied customer...

Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy



On 10/17/09 11:44 AM, "reinhard.rachel-at-biologie.uni-regensburg.de"
{reinhard.rachel-at-biologie.uni-regensburg.de} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} } } }
} } Title-Subject: [Filtered] suppliers for high pressure freezing system
} }
} } Question: Hi,
} }
} } Do you know of suppliers for high pressure freezing system other than
} } Leica microsystem? Thank.
}
} one other supplier is Martin Wohlwend in Switzerland.
} Engineering Office M. Wohlwend
} Bifig 14, CH-9466 Sennwald, Switzerland
} Tel +41 81 7571924
} Fax +41 81 7572243
}
} and: RMC
}
} best regards,
} Reinhard
}




==============================Original Headers==============================
13, 31 -- From dsherman-at-purdue.edu Sat Oct 17 16:14:35 2009
13, 31 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129])
13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9HLEYAf014753
13, 31 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 16:14:35 -0500
13, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126])
13, 31 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n9HLEYYk009663
13, 31 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 17:14:34 -0400
13, 31 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91])
13, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n9HLEYTi031943
13, 31 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2009 17:14:34 -0400
13, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.171]) by WPPEXHUB02F.purdue.lcl
13, 31 -- ([::1]) with mapi; Sat, 17 Oct 2009 17:14:34 -0400
13, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
13, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
13, 31 -- Date: Sat, 17 Oct 2009 17:14:31 -0400
13, 31 -- Subject: Re: [Microscopy] high pressure freezing system
13, 31 -- Thread-Topic: [Microscopy] high pressure freezing system
13, 31 -- Thread-Index: AcpPQL9gffo+xCNAQgy9uRsigSvTdwALhHt2
13, 31 -- Message-ID: {C6FFABF7.172C%dsherman-at-purdue.edu}
13, 31 -- Accept-Language: en-US
13, 31 -- Content-Language: en-US
13, 31 -- X-MS-Has-Attach:
13, 31 -- X-MS-TNEF-Correlator:
13, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
13, 31 -- acceptlanguage: en-US
13, 31 -- Content-Type: text/plain; charset="us-ascii"
13, 31 -- MIME-Version: 1.0
13, 31 -- X-PMX-Version: 5.5.7.378829
13, 31 -- X-PerlMx-Virus-Scanned: Yes
13, 31 -- Content-Transfer-Encoding: 8bit
13, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9HLEYAf014753
==============================End of - Headers==============================




From: samantha_angel_murray-at-hotmail.com
Date: Sun, 18 Oct 2009 09:08:19 -0500
Subject: [Microscopy] viaWWW: Unidentified inclusion bodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both samantha_angel_murray-at-hotmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: samantha_angel_murray-at-hotmail.com
Name: Sam Murray

Organization: Portsmouth University

Title-Subject: [Filtered] Unidentified inclusion bodies

Question: Dear Listservers,

I have TEM glioma spheroid cocultures (glioma v glioma; and glioma v
normal astrocytes, in either human serum (HS) or fetal calf serum
(FCS)), in which there are numerous lipid-like inclusion bodies. I
have attached a link to a web shelf where I have uploaded a windows
Word 2007 document with all images, most of which feature the
inclusion bodies. Is any one able to identify these?

http://tools.iso.port.ac.uk/cgi-bin/shelf?get=186ba870aa2280c62df7574d4d8e37b0

Many thanks




Login Host: 148.197.5.17
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Sun Oct 18 09:08:19 2009
12, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9IE8IJ6026593
12, 11 -- for {microscopy-at-microscopy.com} ; Sun, 18 Oct 2009 09:08:19 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c700d1bf63e5-at-[206.69.208.22]}
12, 11 -- Date: Sun, 18 Oct 2009 09:08:17 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: samantha_angel_murray-at-hotmail.com (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Unidentified inclusion bodies
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Sun, 18 Oct 2009 09:55:14 -0500
Subject: [Microscopy] Re: viaWWW: Unidentified inclusion bodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Samantha

Sorry, can't get the file to open. Can you make it available any other way?

Paul Hazelton

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


==============================Original Headers==============================
5, 20 -- From paul_hazelton-at-umanitoba.ca Sun Oct 18 09:55:14 2009
5, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9IEtEOa010463
5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 18 Oct 2009 09:55:14 -0500
5, 20 -- Received: from [192.168.100.100] (wnpgmb014tw-ad03-189-54.dynamic.mts.net [207.161.189.54])
5, 20 -- (authenticated bits=0)
5, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n9IEtBCj016425;
5, 20 -- Sun, 18 Oct 2009 09:55:12 -0500 (CDT)
5, 20 -- Message-ID: {4ADB2C4D.9080201-at-umanitoba.ca}
5, 20 -- Date: Sun, 18 Oct 2009 09:55:09 -0500
5, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
5, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
5, 20 -- MIME-Version: 1.0
5, 20 -- To: samantha_angel_murray-at-hotmail.com
5, 20 -- Subject: Re: [Microscopy] viaWWW: Unidentified inclusion bodies
5, 20 -- References: {200910181410.n9IEAZK2029331-at-ns.microscopy.com}
5, 20 -- In-Reply-To: {200910181410.n9IEAZK2029331-at-ns.microscopy.com}
5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 20 -- Content-Transfer-Encoding: 7bit
5, 20 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: U.J.Potter-at-bath.ac.uk
Date: Mon, 19 Oct 2009 06:58:57 -0500
Subject: [Microscopy] viaWWW: Job Opportunity-University of Bath

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both U.J.Potter-at-bath.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: U.J.Potter-at-bath.ac.uk
Name: Ursula Potter

Organization: The University of Bath UK

Title-Subject: [Filtered] Job Opportunity

Question: Experimental Officer ñ Electron Microscopy
Salary: £29,704 - £35,469 (depending on experience & qualifications)
The University of Bath UK has an opportunity for
an enthusiastic and qualified electron
microscopist to join the staff of the Centre for
Electron Optical Studies (CEOS) as an
Experimental Officer in the physical sciences.

Please see http://www.bath.ac.uk/jobs/ for more
information or email U.J.Potter-at-bath.ac.uk for
details.

Login Host: 138.38.136.63
---------------------------------------------------------------------------


==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Mon Oct 19 06:58:57 2009
8, 13 -- Received: from [206.69.208.22] ([130.202.238.72])
8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JBwt9f031531
8, 13 -- for {microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 06:58:56 -0500
8, 13 -- Mime-Version: 1.0
8, 13 -- Message-Id: {p06240801c70204e060dc-at-[206.69.208.22]}
8, 13 -- Date: Mon, 19 Oct 2009 06:58:55 -0500
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: U.J.Potter-at-bath.ac.uk (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: Job Opportunity-University of Bath
8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
8, 13 -- Content-Transfer-Encoding: 8bit
8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JBwt9f031531
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Mon, 19 Oct 2009 12:55:18 -0500
Subject: [Microscopy] How to dissolve polymerized Epon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

One of my users, who never listens to what I tell him and argues every point with me, put his waste resin in the wrong oven and now has polymerized SciPoxy 812 all over the inside of my best oven.

The shelves are now epoxied in place as well and the glass vials he processed in are epoxied to the shelf. I told him to put it in the waste oven but Nooooo. I've told him if he cannot clean it he buys me a new oven.

Is there anything that can dissolve polymerized epoxy that won't ruin my oven or the aluminum shelves? Please let me know.

This guy is driving me crazy! I'm sure almost every multiple user facility has a least one of these guys that is nothing but trouble.

Thanks,

Paula

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
12, 20 -- From vapatpxs-at-yahoo.com Mon Oct 19 12:55:18 2009
12, 20 -- Received: from web46104.mail.sp1.yahoo.com (web46104.mail.sp1.yahoo.com [68.180.199.121])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9JHtHcf001410
12, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Oct 2009 12:55:17 -0500
12, 20 -- Received: (qmail 92085 invoked by uid 60001); 19 Oct 2009 17:55:17 -0000
12, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1255974917; bh=WC56FLk2skXi6wzStzmYDnVlUmEONf8OIooP+2Q/Gcs=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=v4x0QN1UVwoRx0aTrW65qImMUjd4A/RsZ4J3g3DGRBmh0hCnwjjjdLHjl3JubeW3TjvClozTZvZIfWky9gniMrdSNHT7mfVExaZjidZvqYffdpyL8inL0nFnEEdtZIbvI6kletDe487LUZ7axtNU5uFcEnQ3DGwN+iEoLsTXXVs=
12, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 20 -- s=s1024; d=yahoo.com;
12, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 20 -- b=4Yrui4sudZHK1qxJQCcyNPIwwdo3rjW8wmPPUBfAMazGzpLCMLJMSghtlt/ZhZ5/GKhOJExE+Qgy30Txz40SWIg9M1kSgo01A42+FRmcMflYFbRYQ+xyrl6AXbdmULIYwS1jFGPro6jY0AtRRTVRTXeiEqQiHedeiSc+334QBgY=;
12, 20 -- Message-ID: {81871.91380.qm-at-web46104.mail.sp1.yahoo.com}
12, 20 -- X-YMail-OSG: SVysAkoVM1mMDH5zYieHGoaa41L53Vh0GgZxYJYT2ZSJl8LaMnK5gISo9yeszWRFHimcCLM9f_qbeRceB5adO.rszHTvnOxSPdAHel27llN_2NukJUfM1FfXkuxNOvmoZkygWGnqSYVAmawssXbUCPhQXNGUsn.HNJnoND2IfqoHEKxreq5VuOohqVk34gkoyBP.FHoaG3fnOZHUeJnZ_181NNFnRO8zwJ4As7ViJhROri72foGVHaDR0HxWkp1DPlPBvM.iDm8IAtEvYyRFE5nV
12, 20 -- Received: from [132.239.85.200] by web46104.mail.sp1.yahoo.com via HTTP; Mon, 19 Oct 2009 10:55:16 PDT
12, 20 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
12, 20 -- Date: Mon, 19 Oct 2009 10:55:16 -0700 (PDT)
12, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 20 -- Subject: How to dissolve polymerized Epon
12, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
12, 20 -- MIME-Version: 1.0
12, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Mon, 19 Oct 2009 14:02:42 -0500
Subject: [Microscopy] Half-baked user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I love it! I'm not alone in dealing with the bad users. If I had a stick I would beat him about the head and shoulders.

He just asked me to teach him how to thick section again! I told him I will charge him double the rates for everything I have to repeat.

He stained some thick sections and told me the resin was bad because the sections were too dark. The resin was fine, he stained too long. Does he believe me? Of course not and he processed a whole new batch of nerve tissue. Did he go long enough on his processing times? Of course not, now he's whining that it didn't work and his tissue looks bad. Duh!

Now he's eyeballing my diamond knives. Quick! Hide them before he uses them.

At least he gives me an appreciation of all the good users.

Giggle,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
12, 20 -- From vapatpxs-at-yahoo.com Mon Oct 19 14:02:42 2009
12, 20 -- Received: from web46114.mail.sp1.yahoo.com (web46114.mail.sp1.yahoo.com [68.180.199.131])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9JJ2fr2018424
12, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Oct 2009 14:02:41 -0500
12, 20 -- Received: (qmail 38687 invoked by uid 60001); 19 Oct 2009 19:02:40 -0000
12, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1255978960; bh=TrO1QM/jC39M6RXQU+o0ghXbISRnVRWjSBeCmm8J3uc=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=FNNRYflWD17cvgFH6zw4gbEL5rY1MwuQ4KLeYWcc9DUgWBMc/TzUJ5JwsMR8mXkVlXJRi0bSCSIIdvFNeZWYeNZvmUUAWZCy1SesJX1t22FCFuWu/GaSpvi+sJ8rDblghQYrSfayHG9BoqT6STgMXNU8cwQjWw1nntpVOy6+6oI=
12, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 20 -- s=s1024; d=yahoo.com;
12, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 20 -- b=JtJ8tQZGF4agZ8v4ZinSwpumt4Ppq/i/T0YjnSNfmvoSziiwNKWl0L87jFWEprGpR48RuI1zLweTv6oBIwj0POI9uexQzK94avOquKxFa410mq3sSVrHlesgBPvLlsZBM6bI4e3EqbVaMNjDmv0VYN8k6cV4m9R5pg3npIsQIp4=;
12, 20 -- Message-ID: {436435.19479.qm-at-web46114.mail.sp1.yahoo.com}
12, 20 -- X-YMail-OSG: AOm9o40VM1lrnQUlPV6VjLzlz.uQR5GcSeM6x7DnrejhzXzOBof.Mba_RNgD7OOl5I18w9Bug1IXQptyvs5yNU4FRJ1ZIJk2q8zWUXOFsHaB26FLX5pgF.EY9zdXghTCx._ypscMBAWauovSlQ3eqo._FStbhaNcaXCUAGtuwlp8w.8E2gVZUL8fcLu8L_8Kyv7qr.XzdIZNka9a2Amn4OaaEdTe0XIOFYELP9dtF_IMfuRiVNcwIqRgArjqQ_RjYOTg065UFJnqk_SqarTKJrLse.gYU4EBc.8ZiDsQZz_fT5P6igLO
12, 20 -- Received: from [132.239.85.200] by web46114.mail.sp1.yahoo.com via HTTP; Mon, 19 Oct 2009 12:02:40 PDT
12, 20 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
12, 20 -- Date: Mon, 19 Oct 2009 12:02:40 -0700 (PDT)
12, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 20 -- Subject: Half-baked user
12, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
12, 20 -- MIME-Version: 1.0
12, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Mon, 19 Oct 2009 15:05:24 -0500
Subject: [Microscopy] Re: Half-baked user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I hate doing one-up man ship. To whom am I lying, I love it.

Paula, I will trade two of him for one of my student who, after 2 years
in a project on molecular epidemiology of gastroenteric viruses could
not tell me why we sequence the amplified genome targets - the whole
reason for his project. Better still, the reason was written on the
blackboard, in large letters for everyone to see when they come into the
lab, and he was facing the blackboard at the time.

In fact, how about one of mine for 3 of yours. And at that I'm robbing
you blind.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Mon Oct 19 15:05:24 2009
6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JK5NNR002917
6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 15:05:24 -0500
6, 20 -- Received: from [192.168.100.100] (wnpgmb014tw-ad03-189-54.dynamic.mts.net [207.161.189.54])
6, 20 -- (authenticated bits=0)
6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n9JK5MZg020295;
6, 20 -- Mon, 19 Oct 2009 15:05:22 -0500 (CDT)
6, 20 -- Message-ID: {4ADCC681.2080103-at-umanitoba.ca}
6, 20 -- Date: Mon, 19 Oct 2009 15:05:21 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: vapatpxs-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com}
6, 20 -- Subject: Re: [Microscopy] Half-baked user
6, 20 -- References: {200910191904.n9JJ4H2E021112-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200910191904.n9JJ4H2E021112-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: bnross-at-interchange.ubc.ca
Date: Mon, 19 Oct 2009 16:40:40 -0500
Subject: [Microscopy] Zeiss EM10C focus/sample height issues.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello again,

We donated our decommissioned Zeiss EM10C to another University about a year ago, and it has now been put back together and fired up for the first time since it was shipped. The physics prof who got the scope up and running managed to get a beam out of it, but that's pretty much where his expertise ended as far as I know. I took a trip up there over the weekend and managed to get the scope pretty well aligned, except that the sample only came into focus right at the very end of the course focus knob travel. Also, (I'm not sure if it's just the way these scopes work) when the standard focus knob is turned, it "resets" back to an initial focus step after a certain number of turns. For example, I could get the sample just to pretty good underfocus, and if I tried to keep turning the knob clockwise to go through focus to overfocus it would reset back to way under focus and repeat if I just kept turning the knob. I couldn't move to the next step on the course focus to correct for that because it was on the last clockwise stop.

My first thought was that the sample height just needs to be adjusted, but I didn't see any obvious way of doing that on the column. Is there a nondescript knob or screw somewhere that allows adjustment of the sample height?

My second thought was that there's something wrong with the objective lens current control(s) because of the weird "resetting" behaviour of the focus knob. Is there a way to check that? Could there be a cable connection that got missed or knocked loose in putting the scope back together after shipping?

If it makes any difference, the scope was set up with STEM capability in the past, so it has the objective lens with the big Leybold ion pumps on the side. I tried inserting a sample with the STEM sample holder, but it didn't seem to fit, and for some reason caused the vacuum to leak rapidly when inserted. The vacuum holds steady with the standard holder.

Also, the "Beam Alignment" knobs are not very clear in their function; I'm guessing one is beam shift or gun shift and the other is gun tilt or gun shift. Can anyone tell me exactly what each set of beam alignment knobs actually controls? Oh, and one more thing, is there a simple way to do C1 alignment, or does it involve allen wrenches and column screws as suggested by the manual?

I'm not very familiar with Zeiss TEM's, so if anyone who owns or has owned/maintained one of these orange beasts could give some helpful hints, that would be great!

Thanks,
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-6996



==============================Original Headers==============================
12, 28 -- From bnross-at-interchange.ubc.ca Mon Oct 19 16:40:40 2009
12, 28 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5])
12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JLeeDU021345
12, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 16:40:40 -0500
12, 28 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
12, 28 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id C4AA218163
12, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 14:40:38 -0700 (PDT)
12, 28 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
12, 28 -- by smtp.interchange.ubc.ca
12, 28 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
12, 28 -- with ESMTP id {0KRS007FQ6VPSS-at-smtp.interchange.ubc.ca} for
12, 28 -- Microscopy-at-microscopy.com; Mon, 19 Oct 2009 14:40:38 -0700 (PDT)
12, 28 -- Date: Mon, 19 Oct 2009 14:40:37 -0700 (PDT)
12, 28 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
12, 28 -- Subject: Zeiss EM10C focus/sample height issues.
12, 28 -- To: Microscopy-at-microscopy.com
12, 28 -- Message-id: {9850435.11511255988437255.JavaMail.myubc2-at-handel.my.ubc.ca}
12, 28 -- MIME-version: 1.0
12, 28 -- X-Mailer: uPortal WEB email client 3.0
12, 28 -- Content-type: text/plain; charset=us-ascii
12, 28 -- X-UBC-Scanned: Sophos PureMessage 5.5.6.374947, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.19.213037
12, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
12, 28 -- X-PerlMx-Spam: Probability=8%, Report=
12, 28 -- SUPERLONG_LINE 0.05, BODY_SIZE_2000_2999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0
12, 28 -- X-Spam-Level:
12, 28 -- X-Spam-Flag: No
12, 28 -- Content-Transfer-Encoding: 8bit
12, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JLeeDU021345
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Mon, 19 Oct 2009 17:03:41 -0500
Subject: [Microscopy] Re: Half-baked user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,
What I told my 4 kids while they were growing up is this:

"Ignorance we can fix, but stupid is forever."

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Monday, October 19, 2009 4:08 PM
To: kenconverse-at-qualityimages.biz

I hate doing one-up man ship. To whom am I lying, I love it.

Paula, I will trade two of him for one of my student who, after 2 years
in a project on molecular epidemiology of gastroenteric viruses could
not tell me why we sequence the amplified genome targets - the whole
reason for his project. Better still, the reason was written on the
blackboard, in large letters for everyone to see when they come into the
lab, and he was facing the blackboard at the time.

In fact, how about one of mine for 3 of yours. And at that I'm robbing
you blind.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Mon Oct 19 15:05:24 2009
6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca
[130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9JK5NNR002917
6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 15:05:24
-0500
6, 20 -- Received: from [192.168.100.100]
(wnpgmb014tw-ad03-189-54.dynamic.mts.net [207.161.189.54])
6, 20 -- (authenticated bits=0)
6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id
n9JK5MZg020295;
6, 20 -- Mon, 19 Oct 2009 15:05:22 -0500 (CDT)
6, 20 -- Message-ID: {4ADCC681.2080103-at-umanitoba.ca}
6, 20 -- Date: Mon, 19 Oct 2009 15:05:21 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: vapatpxs-at-yahoo.com, Microscopy Listserver
{microscopy-at-microscopy.com}
6, 20 -- Subject: Re: [Microscopy] Half-baked user
6, 20 -- References: {200910191904.n9JJ4H2E021112-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200910191904.n9JJ4H2E021112-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




==============================Original Headers==============================
19, 25 -- From kenconverse-at-qualityimages.biz Mon Oct 19 17:03:40 2009
19, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JM3dY2004110
19, 25 -- for {microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 17:03:40 -0500
19, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
19, 25 -- with ESMTP
19, 25 -- id {20091019220337012.JKNN12118-at-cdptpa-omta02.mail.rr.com} ;
19, 25 -- Mon, 19 Oct 2009 22:03:37 +0000
19, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
19, 25 -- To: {paul_hazelton-at-umanitoba.ca} , "MSA Listserver" {microscopy-at-microscopy.com}
19, 25 -- Subject: RE: [Microscopy] Re: Half-baked user
19, 25 -- Date: Mon, 19 Oct 2009 18:03:28 -0400
19, 25 -- Message-ID: {385C93FC70EA4C73A7F960A75B590EF2-at-Ken}
19, 25 -- MIME-Version: 1.0
19, 25 -- Content-Type: text/plain;
19, 25 -- charset="us-ascii"
19, 25 -- X-Priority: 3 (Normal)
19, 25 -- X-MSMail-Priority: Normal
19, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
19, 25 -- Importance: Normal
19, 25 -- Thread-Index: AcpQ99VYEU8ytxTjRkuZmYzJFOebvAAD8r8g
19, 25 -- In-Reply-To: {200910192007.n9JK7lhc006767-at-ns.microscopy.com}
19, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
19, 25 -- Content-Transfer-Encoding: 8bit
19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JM3dY2004110
==============================End of - Headers==============================




From: rbeavers-at-mail.smu.edu
Date: Mon, 19 Oct 2009 17:07:34 -0500
Subject: [Microscopy] E-coli in water image in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Group,

Working on a project outside my experience and could use some help.

Trying to image E-coli in water samples using the QuantomiX WETSEM technology capsules.

The calibration sample from Quantomix works fine with backscatter image showing 500nm and 40nm particles.

Tried raw water sample and had no luck seeing anything. Pretty sure I need to add some contrasting media to water sample but not sure what to add or if that will work. Suggestions from some of you bio prep folks would be greatly appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
9, 27 -- From rbeavers-at-mail.smu.edu Mon Oct 19 17:07:34 2009
9, 27 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JM7X3i012654
9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 17:07:34 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAFuA3EqBd0GE/2dsb2JhbADMTQEJhUSITIJOgWME
9, 27 -- Received: from sxht1p1.systems.smu.edu ([129.119.65.132])
9, 27 -- by smtah2.systems.smu.edu with ESMTP/TLS/RC4-MD5; 19 Oct 2009 17:07:34 -0500
9, 27 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
9, 27 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 19 Oct 2009
9, 27 -- 17:07:31 -0500
9, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
9, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 27 -- Date: Mon, 19 Oct 2009 17:07:30 -0500
9, 27 -- Subject: E-coli in water image in SEM
9, 27 -- Thread-Topic: E-coli in water image in SEM
9, 27 -- Thread-Index: AcpRCI1+dDLCzvjFSVqSLN7yTeUgHQ==
9, 27 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB7E3EA011D5-at-SXMBXC.systems.smu.edu}
9, 27 -- Accept-Language: en-US
9, 27 -- Content-Language: en-US
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- acceptlanguage: en-US
9, 27 -- Content-Type: text/plain; charset="iso-8859-1"
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JM7X3i012654
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Mon, 19 Oct 2009 17:43:04 -0500
Subject: [Microscopy] Epon softener

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everybody,

Thanks for all the support and allowing me to vent.

In the meantime, he was able to restore the oven to usable, if slightly scratched condition.

He used a 1:1 mixture of acetone to chloroform. It softened the epon 812 equivalent enough that he was able to scrape off the resin using a putty knife.

So if this happens to anybody else remember 1:1 acetone : chloroform and lots and lots of elbow grease.

He won't do that again and at least he didn't cut himself when removing the glass vials.

Thanks again!

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
13, 20 -- From vapatpxs-at-yahoo.com Mon Oct 19 17:43:04 2009
13, 20 -- Received: from web46114.mail.sp1.yahoo.com (web46114.mail.sp1.yahoo.com [68.180.199.131])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9JMh4gI001490
13, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Oct 2009 17:43:04 -0500
13, 20 -- Received: (qmail 42073 invoked by uid 60001); 19 Oct 2009 22:43:03 -0000
13, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1255992183; bh=tZuj2BQd7PT2ufWKDpdE2eL2VlnyEvXbW/4JLPLOCXw=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=zZvJMTpFUbn1InRpMRhNDv1q2Dri5d0V7T2w3xGt2yNVQarTuLsRjR1E2ztA0c8lg0xNeNdmMpsyR1ul1dGmwZXNwKVRyWCa6W+Jjo5ZJY4n3lAfmxFgJdoPtV+DZ3PGbS/A3leTJAs6UsxggpmzE4hehx81MNZ2Eh+4ixDD1Mc=
13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 20 -- s=s1024; d=yahoo.com;
13, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 20 -- b=sb6Bf77CMCG+7//0zM/rODCOD49iZXWwZKFNUsdJ+U194HXAHgKHrNbmKMW8hzyrU5qv8+c/UziCTcRjIQqLIzDfroQ4zbWN95vej5kl3tadXPzAayWqUkk7v3zHDCeoLbQX7mCMCNTacw2fWyJxbR4K2XFoGlRmFsxQA7H0AFU=;
13, 20 -- Message-ID: {780662.41599.qm-at-web46114.mail.sp1.yahoo.com}
13, 20 -- X-YMail-OSG: PgJiBj4VM1myriavtamY1kQEh_X2VkuDA0cNAnu80RQ0XVicS3G5FjjPFN0O89P6.HQq49E5vhR1j2yHHba.XI04cN1TqN3qvC6cUU216sS631PdCdD5PXkl1EwtbSrHqwulk5cNLhFt.CscWqrGmk4EEtCyQDxBDe8e3kDOSQkoCbFaHom_PVXPp8lLfsU0ts7jLMdbW_rOsKPoPGjvIGzvgrCqTZvwO3hR1nNn0IzYEf7CQRMN9hLslVnppjIwgDYBMlSWb5hYl1nD4MNJ9Ysv
13, 20 -- Received: from [132.239.85.200] by web46114.mail.sp1.yahoo.com via HTTP; Mon, 19 Oct 2009 15:43:03 PDT
13, 20 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
13, 20 -- Date: Mon, 19 Oct 2009 15:43:03 -0700 (PDT)
13, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 20 -- Subject: Epon softener
13, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Tue, 20 Oct 2009 09:28:15 -0500
Subject: [Microscopy] Sample Prep Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,
I am trying to image liposomes and nanovectors . They are in an
aqueous solution and the samples are separate, not combined. Should I
simply put a drop of the solution on a specimen stub and let it dry?
This is one method I found on Google, just looking for ideas. I have a
conventional SEM. Thanks in advance.

Gary Easton
Scanners Corporation
SEM Service/Sales
410-857-7633
--


==============================Original Headers==============================
3, 19 -- From garyeaston-at-scannerscorp.com Tue Oct 20 09:28:15 2009
3, 19 -- Received: from omr9.networksolutionsemail.com (omr9.networksolutionsemail.com [205.178.146.59])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KESFrD023461
3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 09:28:15 -0500
3, 19 -- Received: from mail.networksolutionsemail.com (mail.networksolutionsemail.com [205.178.146.50])
3, 19 -- by omr9.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n9KESEW9003407
3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 10:28:14 -0400
3, 19 -- Received: (qmail 4262 invoked by uid 78); 20 Oct 2009 14:28:14 -0000
3, 19 -- Received: from unknown (HELO ?192.168.1.3?) (72.81.186.64)
3, 19 -- by ns-omr2.lb.hosting.dc2.netsol.com with SMTP; 20 Oct 2009 14:28:14 -0000
3, 19 -- Message-ID: {4ADDC8BA.4010103-at-scannerscorp.com}
3, 19 -- Date: Tue, 20 Oct 2009 10:27:06 -0400
3, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 19 -- MIME-Version: 1.0
3, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 19 -- Subject: Sample Prep Question
3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 20 Oct 2009 09:36:28 -0500
Subject: [Microscopy] E-coli in water image in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Roy,

In my limited experience with these capsules I always had to add something to enhance contrast since the actual imaging is done with backscattered electrons. Unstained bacteria just don't have the Z-contrast to stand out from their surroundings. I generally used uranyl acetate to stain bacteria, but other heavy metal stains might work as well. The nice thing about the capsules is that you can view the samples first to see what is needed, then add contrasting agent, view the same sample again, take them out and add more stain, if necessary, and continue viewing.

Poor contrast is definitely a problem with this system. Even after using UA, we often had to strongly enhance contrast with imaging programs. This, of course, should always be noted when presenting the images.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
Sent: Monday, October 19, 2009 5:08 PM
To: Tindall, Randy D.

Group,

Working on a project outside my experience and could use some help.

Trying to image E-coli in water samples using the QuantomiX WETSEM technology capsules.

The calibration sample from Quantomix works fine with backscatter image showing 500nm and 40nm particles.

Tried raw water sample and had no luck seeing anything. Pretty sure I need to add some contrasting media to water sample but not sure what to add or if that will work. Suggestions from some of you bio prep folks would be greatly appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
9, 27 -- From rbeavers-at-mail.smu.edu Mon Oct 19 17:07:34 2009
9, 27 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JM7X3i012654
9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 17:07:34 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAFuA3EqBd0GE/2dsb2JhbADMTQEJhUSITIJOgWME
9, 27 -- Received: from sxht1p1.systems.smu.edu ([129.119.65.132])
9, 27 -- by smtah2.systems.smu.edu with ESMTP/TLS/RC4-MD5; 19 Oct 2009 17:07:34 -0500
9, 27 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
9, 27 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 19 Oct 2009
9, 27 -- 17:07:31 -0500
9, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
9, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 27 -- Date: Mon, 19 Oct 2009 17:07:30 -0500
9, 27 -- Subject: E-coli in water image in SEM
9, 27 -- Thread-Topic: E-coli in water image in SEM
9, 27 -- Thread-Index: AcpRCI1+dDLCzvjFSVqSLN7yTeUgHQ==
9, 27 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB7E3EA011D5-at-SXMBXC.systems.smu.edu}
9, 27 -- Accept-Language: en-US
9, 27 -- Content-Language: en-US
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- acceptlanguage: en-US
9, 27 -- Content-Type: text/plain; charset="iso-8859-1"
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JM7X3i012654
==============================End of - Headers==============================


==============================Original Headers==============================
23, 33 -- From TindallR-at-missouri.edu Tue Oct 20 09:36:28 2009
23, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
23, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KEaRjf001231
23, 33 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 09:36:27 -0500
23, 33 -- X-IronPort-Anti-Spam-Filtered: true
23, 33 -- X-IronPort-Anti-Spam-Result: ApoEALPi1krRauUo/2dsb2JhbADLJgEJhmaISYJOgWIEgVuJEw
23, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
23, 33 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Oct 2009 09:36:27 -0500
23, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
23, 33 -- Tue, 20 Oct 2009 09:36:27 -0500
23, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
23, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Tue, 20 Oct 2009
23, 33 -- 09:36:22 -0500
23, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 33 -- To: "'rbeavers-at-mail.smu.edu'" {rbeavers-at-mail.smu.edu}
23, 33 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
23, 33 -- Date: Tue, 20 Oct 2009 09:36:22 -0500
23, 33 -- Subject: RE: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Topic: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Index: AcpRCKmJBPCdwfqPTsK18bjNEK6C1AAiSalQ
23, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B81299905642-at-UM-EMAIL06.um.umsystem.edu}
23, 33 -- References: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- In-Reply-To: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- Accept-Language: en-US
23, 33 -- Content-Language: en-US
23, 33 -- X-MS-Has-Attach:
23, 33 -- X-MS-TNEF-Correlator:
23, 33 -- acceptlanguage: en-US
23, 33 -- Content-Type: text/plain; charset="iso-8859-1"
23, 33 -- MIME-Version: 1.0
23, 33 -- X-OriginalArrivalTime: 20 Oct 2009 14:36:27.0021 (UTC) FILETIME=[B494DBD0:01CA5192]
23, 33 -- Content-Transfer-Encoding: 8bit
23, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9KEaRjf001231
==============================End of - Headers==============================




From: trommoni-at-isu.edu
Date: Tue, 20 Oct 2009 09:57:13 -0500
Subject: [Microscopy] SEM - Pacific phytoliths, pollens and starches

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I am a student at Idaho State University working on a project that
involves identifying mostly phytoliths from Easter Island. We are
trying to build up a key of images for the Pacific to help in the id
process. We have found quite a few LM pictures, but were hoping to
find more SEM pictures. Does anyone know if/where there is a
collection of SEM phytolith pictures?

Thanks!

Monica Tromp
Anthropology Department
Idaho State University

==============================Original Headers==============================
4, 21 -- From trommoni-at-isu.edu Tue Oct 20 09:57:11 2009
4, 21 -- Received: from mail-pz0-f194.google.com (mail-pz0-f194.google.com [209.85.222.194])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KEvAX9020771
4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 09:57:11 -0500
4, 21 -- Received: by pzk32 with SMTP id 32so5439046pzk.21
4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 07:57:10 -0700 (PDT)
4, 21 -- Received: by 10.115.61.10 with SMTP id o10mr9494320wak.37.1256050630391;
4, 21 -- Tue, 20 Oct 2009 07:57:10 -0700 (PDT)
4, 21 -- Received: from ?10.0.1.5? (72-24-93-251.cpe.cableone.net [72.24.93.251])
4, 21 -- by mx.google.com with ESMTPS id 21sm13818pxi.0.2009.10.20.07.57.08
4, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
4, 21 -- Tue, 20 Oct 2009 07:57:09 -0700 (PDT)
4, 21 -- From: Monica Tromp {trommoni-at-isu.edu}
4, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
4, 21 -- Content-Transfer-Encoding: 7bit
4, 21 -- Subject: SEM - Pacific phytoliths, pollens and starches
4, 21 -- Date: Tue, 20 Oct 2009 08:57:07 -0600
4, 21 -- Message-Id: {6D686EF0-F3D5-47A4-88E4-5EB827DE9B18-at-isu.edu}
4, 21 -- To: Microscopy-at-microscopy.com
4, 21 -- Mime-Version: 1.0 (Apple Message framework v1076)
4, 21 -- X-Mailer: Apple Mail (2.1076)
==============================End of - Headers==============================




From: mike-at-bitplane.com
Date: Tue, 20 Oct 2009 15:09:00 -0500
Subject: [Microscopy] Position Opening Sales Specialist Bitplane Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bitplane AG is a world leader in 3-D and 4-D imaging software for the
Life Sciences Industry. Its North American operations (headquartered
in Saint Paul, MN) is seeking qualified candidates for the position of
Sales Specialist for two different regions.

Additional information on these positions can be found at:
http://www.bitplane.com/go/bitplane/jobs

Position Opening Sales Specialist
(Territory of ND, SD, MN, IA, WI, IL, MI, IN, OH, PA)

This position will provide direct sales interaction with Bitplane's
US potential customer base for its complete range of software
products. Candidates will have the opportunity to develop the market
in their geographic region. Position provides ample opportunity for
rapid advancement and increased earning potential. Position is ideal
for candidates that enjoy travel but can also work easily out of their
home office in their region.

The requirements for this position are:

*Confocal, widefield, live-cell and/or digital imaging experience
*Experience with microscopy image data processing
*Outgoing personality with an affinity for self-motivation and responsibility
*Excellent communication skills, oral, and written
*Ability to vertically interface with multiple levels of diverse
professional clients

Desirable, but not required experience:

*Technical knowledge of PC hardware, subsystems, peripheral and
networking equipment
*Degree in biology or life sciences
*Prior experience in a sales capacity

The duties of this position include:

*Customer visits and analysis of customer's imaging needs.
*Demonstration of the software and onsite work with the customer
*Organization of exhibitions and workshops
*Sales support of existing customers

At least 50% travel will be required. Representative is required to
live in the territory they cover. Benefits include a base salary,
performance based commission, 401K plan, healthcare, and vacation.
Representative will work out of a home office.

Position Opening Sales Specialist US Based
(Territory of India, select South East Asian countries)

Candidates will have the opportunity to develop the market via direct
sales or through the use of distributors primarily in India, Korea,
Singapore, Thailand, Twain, but also potentially in other countries
that Bitplane does not currently serve directly. Position provides
ample opportunity for rapid advancement and increased earning
potential. Position is ideal for candidates that enjoy international
travel but can also work easily out of their home office.

The requirements for this position are:

*Confocal, widefield, live-cell and/or digital imaging experience
*Experience with microscopy image data processing
*Outgoing personality with an affinity for self-motivation and responsibility
*Excellent communication skills, oral, and written
*Ability to vertically interface with multiple levels of diverse
professional clients

Desirable, but not required experience:

*Technical knowledge of PC hardware, subsystems, peripheral and
networking equipment
*Degree in biology or life sciences
*Prior experience in a sales capacity
*Fluency or working knowledge of the Hindi language
*Knowledge of the Indian and / or South East Asian research community

The duties of this position include:

*Customer visits and analysis of customer's imaging needs.
*Demonstration of the software and onsite work with the customer
*Organization of exhibitions and workshops
*Potentially developing and supporting a dealer network in identified
countries

At least 50% travel will be required. Representative may live in any
part of the US but must be near a major airport. Benefits include a
base salary, performance based commission, 401K plan, healthcare, and
vacation.

If you are interested in joining this team of professionals and your
qualifications meet these requirements; send us your resume and cover
letter for consideration. Please include your salary history and/or
salary requirements. Compensation will be commensurate with relevant
experience.

Respond to:

Michael C. Wussow
VP & General Manager
Bitplane Inc.
e-mail: jobinfo-at-bitplane.com
facsimile: 866-691-9112



==============================Original Headers==============================
25, 26 -- From mike-at-bitplane.com Tue Oct 20 15:09:00 2009
25, 26 -- Received: from mail30.mailforbusiness.com (mail30.mailforbusiness.com [64.106.209.55])
25, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KK8vCW015065
25, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 15:09:00 -0500
25, 26 -- Received: from mail30.mailforbusiness.com (localhost [127.0.0.1])
25, 26 -- by mail30.mailforbusiness.com (Postfix) with ESMTP id 486723C676
25, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 16:08:56 -0400 (EDT)
25, 26 -- Received: by mail30.mailforbusiness.com (Postfix, from userid 80)
25, 26 -- id 441D13C6C9; Tue, 20 Oct 2009 16:08:56 -0400 (EDT)
25, 26 -- Received: from c-71-201-152-64.hsd1.il.comcast.net
25, 26 -- (c-71-201-152-64.hsd1.il.comcast.net [71.201.152.64]) by mail.bitplane.com
25, 26 -- (Horde Framework) with HTTP; Tue, 20 Oct 2009 16:08:56 -0400
25, 26 -- Message-ID: {20091020160856.13161n58ge5fdwis-at-webmail30.mailforbusiness.com}
25, 26 -- Date: Tue, 20 Oct 2009 16:08:56 -0400
25, 26 -- From: mike-at-bitplane.com
25, 26 -- To: Microscopy-at-microscopy.com
25, 26 -- Subject: Position Opening Sales Specialist Bitplane Inc.
25, 26 -- MIME-Version: 1.0
25, 26 -- Content-Type: text/plain;
25, 26 -- charset=ISO-8859-1;
25, 26 -- DelSp="Yes";
25, 26 -- format="flowed"
25, 26 -- Content-Disposition: inline
25, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.3)
25, 26 -- Content-Transfer-Encoding: 8bit
25, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9KK8vCW015065
==============================End of - Headers==============================




From: mike-at-bitplane.com
Date: Tue, 20 Oct 2009 15:14:09 -0500
Subject: [Microscopy] Position Opening Technical Support Specialist Bitplane Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Position Opening Technical Support Specialist

Further information on this position can be found here:
http://www.bitplane.com/go/bitplane/jobs

Bitplane AG is a world leader in 3-D and 4-D imaging software for the
Life Sciences Industry. Its North American operations (headquartered
in Saint Paul, MN) is seeking qualified candidates for the position of
Technical Support Specialist. We are looking for a candidate with
strong computer skills and at least one year of hands-on working
experience using a confocal or similar 3D advanced light microscope.
Applicants must be excited by the latest computer and microscope
technologies and have a well-founded biological knowledge base.
Candidates must enjoy the challenges of working in a dynamic market
where products, research technologies, and demands are continually
evolving.

The requirements for this position are:

*You have worked with a confocal microscope (or other 3D light
microscope) studying biological samples.
*You are a self-motivated individual who is able to work independently
and capable of managing multiple projects.
*In-depth knowledge of and proficiency with Microsoft Windows
*Technical knowledge of PC hardware, subsystems, peripheral and
networking equipment
*Strong written and verbal communication skills in English with an
adequate technical and biological vocabulary, and natural ability to
adapt communication style to interface with clients in a wide range of
positions and experience levels
*Strong interpersonal skills and excellent customer services ethic
*You have had a prior position where you could demonstrate your
problem solving skills.
*You are already located in the eastern half of the US, or willing to
relocate there. Some additional geographic details to be discussed,
but specific city and state of residence is very flexible overall.

Desirable, but not required experience:

*Prior experience using Imaris, or other software with comparable features.
*Proficiency with Mac OSX
*Basic knowledge in Matlab

The duties of this position include:

*Technical support for 3D Imaging analysis software for microscopy.
*Pro-active communication with customers via telephone and email to
ensure that you understand the issue and they understand the resolution.
*You will combine product understanding, technical skills, and
biological know-how to investigate problems at hand and to provide an
optimal solution to the customer.
*You will also assist our sales-team with preparing customer data for
a sales presentation.
*Reporting and cataloguing software problems, bugs and feature
requests; including testing of pre-release (alpha/beta) versions of
the software

This job requires you to perform on-site customer trainings, represent
Bitplane at courses & meetings, and involves approximately 10-20% of
time travelling.
Benefits include a base salary, 401K plan, healthcare, and vacation.
Representative will work out of a home office. In addition, we offer a
fun team to work with as well as a truly international environment
that provides the resources required to grow. Bitplane will fully
cover your expenses for equipment, internet access, telephone, and
travel.


If you are interested in joining this team of professionals and your
qualifications meet these requirements, send us your resume and cover
letter for consideration. Please include your salary history and/or
salary requirements. Compensation will be commensurate with relevant
experience.

Respond to:

Michael C. Wussow
VP & General Manager
Bitplane Inc.
e-mail: jobinfo-at-bitplane.com
facsimile: 866-691-9112



==============================Original Headers==============================
16, 26 -- From mike-at-bitplane.com Tue Oct 20 15:14:08 2009
16, 26 -- Received: from mail30.mailforbusiness.com (mail30.mailforbusiness.com [64.106.209.55])
16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KKE7l0017762
16, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 15:14:08 -0500
16, 26 -- Received: from mail30.mailforbusiness.com (localhost [127.0.0.1])
16, 26 -- by mail30.mailforbusiness.com (Postfix) with ESMTP id 30DE53C665
16, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 16:14:07 -0400 (EDT)
16, 26 -- Received: by mail30.mailforbusiness.com (Postfix, from userid 80)
16, 26 -- id 2C9333C76D; Tue, 20 Oct 2009 16:14:07 -0400 (EDT)
16, 26 -- Received: from c-71-201-152-64.hsd1.il.comcast.net
16, 26 -- (c-71-201-152-64.hsd1.il.comcast.net [71.201.152.64]) by mail.bitplane.com
16, 26 -- (Horde Framework) with HTTP; Tue, 20 Oct 2009 16:14:07 -0400
16, 26 -- Message-ID: {20091020161407.12767vt83ljmfxmo-at-webmail30.mailforbusiness.com}
16, 26 -- Date: Tue, 20 Oct 2009 16:14:07 -0400
16, 26 -- From: mike-at-bitplane.com
16, 26 -- To: Microscopy-at-microscopy.com
16, 26 -- Subject: Position Opening Technical Support Specialist Bitplane Inc.
16, 26 -- MIME-Version: 1.0
16, 26 -- Content-Type: text/plain;
16, 26 -- charset=ISO-8859-1;
16, 26 -- DelSp="Yes";
16, 26 -- format="flowed"
16, 26 -- Content-Disposition: inline
16, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.3)
16, 26 -- Content-Transfer-Encoding: 8bit
16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9KKE7l0017762
==============================End of - Headers==============================




From: Joan.Sempf-at-va.gov
Date: Tue, 20 Oct 2009 20:02:25 -0500
Subject: [Microscopy] viaWWW: Osmium Tetroxide Waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Joan.Sempf-at-va.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Joan.Sempf-at-va.gov
Name: Joan Sempf

Organization: VA Hospital

Title-Subject: [Filtered] Osmium Tetroxide

Question: I am looking to see how other
facilities handle their waste Osmium tetroxide.
We use Caulfieldís fixative containing 2% Osmium
and were audited by the EPA. Osmium tetroxide is
classified by the EPA as a P4 waste and we are
trying to figure out the best way to dispose of
it. We were neutralizing it with corn oil and
disposing down the drain. Another question I
have is if you consider the transfer containers
and pipettes as P listed waste? The EPA does not
want us doing that any longer. Any suggestions
are welcome.

Login Host: 152.132.8.198
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Tue Oct 20 20:02:24 2009
7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9L12Nkq021448
7, 13 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 20:02:23 -0500
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240806c7040dff86af-at-[206.69.208.22]}
7, 13 -- Date: Tue, 20 Oct 2009 20:02:22 -0500
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: Joan.Sempf-at-va.gov (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: Osmium Tetroxide Waste
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9L12Nkq021448
==============================End of - Headers==============================




From: eikonika-at-otenet.gr
Date: Wed, 21 Oct 2009 00:16:06 -0500
Subject: [Microscopy] liposome preparation for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gary

We prepare for SEM liposomes, nanoemulsions and other nanoparticles by
placing them
on a filter paper. Please have a look:

Scanning electron microscopy study on nanoemulsions and solid lipid
nanoparticles containing high amounts of ceramides.
Hatziantoniou S, Deli G, Nikas Y, Demetzos C, Papaioannou GT.
Micron. 2007;38(8):819-23. Epub 2007 Jul 3.

Solid lipid nanoparticles and nanoemulsions containing ceramides:
Preparation and physicochemical characterization.
Deli G, Hatziantoniou S, Nikas Y, Demetzos C.
J Liposome Res. 2009;19(3):180-8.


Regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

eikonika-at-otenet.gr
yorgosnikas-at-hotmail.com
Tel/fax +30 210 8957677
Mobile +30 6945 107477


==============================Original Headers==============================
9, 22 -- From eikonika-at-otenet.gr Wed Oct 21 00:16:06 2009
9, 22 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [83.235.67.30])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9L5G4bk012727
9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 00:16:05 -0500
9, 22 -- Received: from axeron (athedsl-259690.home.otenet.gr [85.73.62.8])
9, 22 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id n9L5G33r028240
9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 08:16:03 +0300
9, 22 -- Message-ID: {002101ca520d$96533b50$1601a8c0-at-axeron}
9, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr}
9, 22 -- To: {microscopy-at-microscopy.com}
9, 22 -- Subject: liposome preparation for SEM
9, 22 -- Date: Wed, 21 Oct 2009 08:16:03 +0300
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain;
9, 22 -- format=flowed;
9, 22 -- charset="iso-8859-7";
9, 22 -- reply-type=original
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-Priority: 3
9, 22 -- X-MSMail-Priority: Normal
9, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
9, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




From: Ann-Fook.Yang-at-AGR.GC.CA
Date: Wed, 21 Oct 2009 08:43:18 -0500
Subject: [Microscopy] High pressure freezer system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I like to thank those who replied to my question. All the answers,
on/off line, pointed to Balzers/Bal-tec instrument.

Now, I would appreciate it if those who have a Leica EM PACT2 (with or
without RTS) High Pressure Freezer system to comment on its performance,
reliability, and maintenance issue.

Thank you in advance.

Ann Fook
} -----------------------------------------------------------------------
-----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


==============================Original Headers==============================
5, 23 -- From Ann-Fook.Yang-at-AGR.GC.CA Wed Oct 21 08:43:17 2009
5, 23 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118])
5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LDhHvb007228
5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 08:43:17 -0500
5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 23 -- Content-class: urn:content-classes:message
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Type: text/plain;
5, 23 -- charset="us-ascii"
5, 23 -- Subject: High pressure freezer system
5, 23 -- Date: Wed, 21 Oct 2009 09:43:15 -0400
5, 23 -- Message-ID: {773CE84BBA86484A9FEDE7741B77DDA4016174D9-at-onottaxms1.AGR.GC.CA}
5, 23 -- In-Reply-To: {f06240801c700da4b544e-at-[141.209.160.249]}
5, 23 -- X-MS-Has-Attach:
5, 23 -- X-MS-TNEF-Correlator:
5, 23 -- Thread-Topic: High pressure freezer system
5, 23 -- Thread-Index: AcpQAi9JBgkaQOHNSf2IZwV0BgMv7gCSVamQ
5, 23 -- From: "Yang, Ann-Fook" {Ann-Fook.Yang-at-AGR.GC.CA}
5, 23 -- To: {microscopy-at-microscopy.com}
5, 23 -- X-OriginalArrivalTime: 21 Oct 2009 13:43:15.0144 (UTC) FILETIME=[707CD480:01CA5254]
5, 23 -- Received-SPF: none
5, 23 -- Content-Transfer-Encoding: 8bit
5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LDhHvb007228
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 21 Oct 2009 08:44:09 -0500
Subject: [Microscopy] viaWWW: Unidentified inclusion bodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To those who want to download this file, take care that it is 30 MB big (40 pages of TEM pictures).

To Sam:

These look like lipid droplets. Not being a neurology specialist I made a google search with lipid droplets and glioma and found that:

http://cat.inist.fr/?aModele=afficheN&cpsidt=2573160

Which is pretty consistent with what we can see in your pictures.

Some of your pictures do not show the lipid droplets. In this case I don't know what you are looking for.
(f.ex. page 34 shows a completely normal cell, nothing specific)

Knowing how you prepared your material would be incredibly useful to understand what we see.

Apart from that, some picture clearly show preparation artifacts, whether bad fixation or incomplete dehydration.
If you had difficulty in cutting thin sections, my bet would be incomplete dehydration.

regards,

Stephane




----- Original Message ----
X-from: "samantha_angel_murray-at-hotmail.com" {samantha_angel_murray-at-hotmail.com}
To: nizets2-at-yahoo.com
Sent: Sun, October 18, 2009 4:14:44 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both samantha_angel_murray-at-hotmail.com as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: samantha_angel_murray-at-hotmail.com
Name: Sam Murray

Organization: Portsmouth University

Title-Subject: [Filtered] Unidentified inclusion bodies

Question: Dear Listservers,

I have TEM glioma spheroid cocultures (glioma v glioma; and glioma v
normal astrocytes, in either human serum (HS) or fetal calf serum
(FCS)), in which there are numerous lipid-like inclusion bodies. I
have attached a link to a web shelf where I have uploaded a windows
Word 2007 document with all images, most of which feature the
inclusion bodies. Is any one able to identify these?

http://tools.iso.port.ac.uk/cgi-bin/shelf?get=186ba870aa2280c62df7574d4d8e37b0

Many thanks




  Login Host: 148.197.5.17
---------------------------------------------------------------------------

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Sun Oct 18 09:08:19 2009
12, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
12, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9IE8IJ6026593
12, 11 --     for {microscopy-at-microscopy.com} ; Sun, 18 Oct 2009 09:08:19 -0500
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06240800c700d1bf63e5-at-[206.69.208.22]}
12, 11 -- Date: Sun, 18 Oct 2009 09:08:17 -0500
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: samantha_angel_murray-at-hotmail.com (by way of MicroscopyListserver)
12, 11 -- Subject: viaWWW: Unidentified inclusion bodies
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================







==============================Original Headers==============================
36, 31 -- From nizets2-at-yahoo.com Wed Oct 21 08:44:09 2009
36, 31 -- Received: from n69.bullet.mail.sp1.yahoo.com (n69.bullet.mail.sp1.yahoo.com [98.136.44.41])
36, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9LDi8TV008244
36, 31 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 08:44:08 -0500
36, 31 -- Received: from [216.252.122.219] by n69.bullet.mail.sp1.yahoo.com with NNFMP; 21 Oct 2009 13:44:07 -0000
36, 31 -- Received: from [67.195.9.82] by t4.bullet.sp1.yahoo.com with NNFMP; 21 Oct 2009 13:44:07 -0000
36, 31 -- Received: from [67.195.9.100] by t2.bullet.mail.gq1.yahoo.com with NNFMP; 21 Oct 2009 13:44:07 -0000
36, 31 -- Received: from [127.0.0.1] by omp104.mail.gq1.yahoo.com with NNFMP; 21 Oct 2009 13:44:07 -0000
36, 31 -- X-Yahoo-Newman-Property: ymail-3
36, 31 -- X-Yahoo-Newman-Id: 766722.46922.bm-at-omp104.mail.gq1.yahoo.com
36, 31 -- Received: (qmail 69694 invoked by uid 60001); 21 Oct 2009 13:44:07 -0000
36, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1256132647; bh=yORaWtHKOYmZu/WaU3pe11vpxMHnw144PjTSqcZ+l+E=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=kYjl8VZSRMSA6u9PikvDSBKtbo/b6mdtSXjyr1ijhl4JoPKg003oYXcnAaPBW01GAZGFM9I9DVdRKGfKMONLgw6hRw5Ytjq4e6mlNkhfcT9Hoo8ZP8mARDB7Z+p2VBPYXhHoUYnoNRdT4Y1jng46Xw51Kt3bMgknmEisZHYkKL4=
36, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
36, 31 -- s=s1024; d=yahoo.com;
36, 31 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
36, 31 -- b=Ufp8GApurZ56g6gBvwZay+Wur4XoBH0uOKPcwWtZrvgL125/hWm5c0TYIZ+TL1kK9bJz7XCcZ9uxUCqNQ9cv1f8jB3/07lv8ErpeN1JsskH9prp4BPf5UK6YEj0dJ5ym8U8GS8J4O5tGVRyCPdLSrTce+Oa1IRL7Weu5fGJkeJI=;
36, 31 -- Message-ID: {623405.69473.qm-at-web110803.mail.gq1.yahoo.com}
36, 31 -- X-YMail-OSG: zfrc6J4VM1lbAi_8oh0wfNT3oYFyK4AiQiTxOgrtQVo3QbiSn2.Ol_KTYuZ1UThCA4DsCh6BAMhRfdWbbSd7NW8ikIOmCiLL2Jolp6Ogwsyczpf8Kgr00GwMpegFky7LsxmB5xbJVwSpyJ6cTIn4_BwfpCz_ZJuSj8ADjxN7j0s4S1u9UI3QIhHdycQzla8vca_IyHzKC3bcobqfJlSlct7haqTH3VgrTQQ8mf6_iS2mAYUZTpYN0oxmmsw3Rz91rH41kGLf2E2A59ZtBVW70pk6R3FDrLYj6B5S6hql4uE0cjzuuP5yIj0oSMDLBBCg1Lilg_Zo5._ZtxJbIi9raX2bAimrn3LXB.ZdoeTBWtXRabUTvqJHX6hgxQ--
36, 31 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Wed, 21 Oct 2009 06:44:07 PDT
36, 31 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
36, 31 -- References: {200910181414.n9IEEi1l000637-at-ns.microscopy.com}
36, 31 -- Date: Wed, 21 Oct 2009 06:44:07 -0700 (PDT)
36, 31 -- From: Stephane Nizet {nizets2-at-yahoo.com}
36, 31 -- Subject: Re: [Microscopy] viaWWW: Unidentified inclusion bodies
36, 31 -- To: samantha_angel_murray-at-hotmail.com
36, 31 -- Cc: microscopy-at-microscopy.com
36, 31 -- In-Reply-To: {200910181414.n9IEEi1l000637-at-ns.microscopy.com}
36, 31 -- MIME-Version: 1.0
36, 31 -- Content-Type: text/plain; charset=iso-8859-1
36, 31 -- Content-Transfer-Encoding: 8bit
36, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LDi8TV008244
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 21 Oct 2009 09:06:09 -0500
Subject: [Microscopy] E-coli in water image in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would it be possible to contrast the bacteria samples with osmium tetroxyde?

I would like to use the opportunity to ask a question about the QuantomiX WETSEM technology capsules.
Imaging eucaryotic cells in SEM is a real problem mainly due to the crumbling of the cells under vacuum.
Would it be realistic to image cells in buffer using these technology advanced-very expensive capsules in "near-life conditions" after fixation and osmium tetroxide contrasting? Would it be possible to image them in SE modus? Why not?

Regards,

Stephane



----- Original Message ----
X-from: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
To: nizets2-at-yahoo.com
Sent: Tue, October 20, 2009 4:41:25 PM

Hi Roy,

In my limited experience with these capsules I always had to add something to enhance contrast since the actual imaging is done with backscattered electrons.  Unstained bacteria just don't have the Z-contrast to stand out from their surroundings.  I generally used uranyl acetate to stain bacteria, but other heavy metal stains might work as well.  The nice thing about the capsules is that you can view the samples first to see what is needed, then add contrasting agent, view the same sample again, take them out and add more stain, if necessary, and continue viewing.

Poor contrast is definitely a problem with this system.  Even after using UA, we often had to strongly enhance contrast with imaging programs.  This, of course, should always be noted when presenting the images.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway:  http://www.sofn.com




-----Original Message-----
X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
Sent: Monday, October 19, 2009 5:08 PM
To: Tindall, Randy D.

Group,

Working on a project outside my experience and could use some help.

Trying to image E-coli in water samples using the QuantomiX WETSEM technology capsules.

The calibration sample from Quantomix works fine with backscatter image showing 500nm and 40nm particles.

Tried raw water sample and had no luck seeing anything. Pretty sure I need to add some contrasting media to water sample but not sure what to add or if that will work. Suggestions from some of you bio prep folks would be greatly appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
9, 27 -- From rbeavers-at-mail.smu.edu Mon Oct 19 17:07:34 2009
9, 27 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151])
9, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JM7X3i012654
9, 27 --     for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 17:07:34 -0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAFuA3EqBd0GE/2dsb2JhbADMTQEJhUSITIJOgWME
9, 27 -- Received: from sxht1p1.systems.smu.edu ([129.119.65.132])
9, 27 --  by smtah2.systems.smu.edu with ESMTP/TLS/RC4-MD5; 19 Oct 2009 17:07:34 -0500
9, 27 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
9, 27 --  sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 19 Oct 2009
9, 27 --  17:07:31 -0500
9, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
9, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 27 -- Date: Mon, 19 Oct 2009 17:07:30 -0500
9, 27 -- Subject: E-coli in water image in SEM
9, 27 -- Thread-Topic: E-coli in water image in SEM
9, 27 -- Thread-Index: AcpRCI1+dDLCzvjFSVqSLN7yTeUgHQ==
9, 27 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB7E3EA011D5-at-SXMBXC.systems.smu.edu}
9, 27 -- Accept-Language: en-US
9, 27 -- Content-Language: en-US
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- acceptlanguage: en-US
9, 27 -- Content-Type: text/plain; charset="iso-8859-1"
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JM7X3i012654
==============================End of - Headers==============================


==============================Original Headers==============================
23, 33 -- From TindallR-at-missouri.edu Tue Oct 20 09:36:28 2009
23, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
23, 33 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9KEaRjf001231
23, 33 --     for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 09:36:27 -0500
23, 33 -- X-IronPort-Anti-Spam-Filtered: true
23, 33 -- X-IronPort-Anti-Spam-Result: ApoEALPi1krRauUo/2dsb2JhbADLJgEJhmaISYJOgWIEgVuJEw
23, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
23, 33 --  by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Oct 2009 09:36:27 -0500
23, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
23, 33 --     Tue, 20 Oct 2009 09:36:27 -0500
23, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
23, 33 --  UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Tue, 20 Oct 2009
23, 33 --  09:36:22 -0500
23, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 33 -- To: "'rbeavers-at-mail.smu.edu'" {rbeavers-at-mail.smu.edu}
23, 33 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
23, 33 -- Date: Tue, 20 Oct 2009 09:36:22 -0500
23, 33 -- Subject: RE: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Topic: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Index: AcpRCKmJBPCdwfqPTsK18bjNEK6C1AAiSalQ
23, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B81299905642-at-UM-EMAIL06.um.umsystem.edu}
23, 33 -- References: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- In-Reply-To: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- Accept-Language: en-US
23, 33 -- Content-Language: en-US
23, 33 -- X-MS-Has-Attach:
23, 33 -- X-MS-TNEF-Correlator:
23, 33 -- acceptlanguage: en-US
23, 33 -- Content-Type: text/plain; charset="iso-8859-1"
23, 33 -- MIME-Version: 1.0
23, 33 -- X-OriginalArrivalTime: 20 Oct 2009 14:36:27.0021 (UTC) FILETIME=[B494DBD0:01CA5192]
23, 33 -- Content-Transfer-Encoding: 8bit
23, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9KEaRjf001231
==============================End of - Headers==============================






==============================Original Headers==============================
40, 24 -- From nizets2-at-yahoo.com Wed Oct 21 09:06:09 2009
40, 24 -- Received: from web110812.mail.gq1.yahoo.com (web110812.mail.gq1.yahoo.com [67.195.13.235])
40, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9LE68cN004649
40, 24 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 09:06:09 -0500
40, 24 -- Received: (qmail 842 invoked by uid 60001); 21 Oct 2009 14:06:08 -0000
40, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1256133968; bh=vIJJ3ISVyC83CS7Z9JmH+36ksaQiEaPbXfaPknxFkRE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=Jdnq5RA80FNxgXc51nZWXyRnErc3S1AWqOg4qCScJcM3H7R54XXdKFlcT741T24fRB//8DZZ35uTl5FRpCObwys5YZ1RrvBF7Qoq2n4LjASQbFh5F64bXbw+bWlA2X3vzsCJVKjSKT0it0l7T+pTjXZ+cq9D/V8n4eVsOSlr15o=
40, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
40, 24 -- s=s1024; d=yahoo.com;
40, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
40, 24 -- b=FQg3PsHit41iRi+9jIJkNc7ZS3AGv/pQaGqnI4hpZs45xhmwso+RcStvNatM+aR7rAn1G1g7DiQqJ0JOzthWCfI7UjI8Vza2YGrzSOhlq0CGWvYXXWE4e4qgImNbdBqyBSSugXR7kHsl7ryVWULCepz1yL2ZX3xCOcLPtqGXjE4=;
40, 24 -- Message-ID: {371324.99658.qm-at-web110812.mail.gq1.yahoo.com}
40, 24 -- X-YMail-OSG: 7qryGP0VM1kVR7WF_BXqC7yZJgVsjmo9nvwrW05QSe.eSC0jvkBjD_OnGz7usSKoFbg8VeK8bT.ytZc4WwRw4YhTcUklfa7BvxZqhsd5U8_CFwr_E7Vj3Z9KnCoHB_2KR5WEc9TG6S1ug8GrhKIBMN9zg5d3KSQFP4tVTp35NMInBxHPoxt9dGdJxivpEr8tU_DPCdUzVMgC1Km5WDHVTbzzBjACD18gImYCccILi.dKa8MkwQZG08r2rmSggoAHw_pZIHmnsXD2GjJqezRrWzxI9nDeKOhTnQnd2NIusrHzWYIQcxVNG3B9oW5_pOFFLEZqEvaxd93ztVyRFDt60IMDYQuMsliXArzfsa3Y_I4wMruq9v4POWS9qQ--
40, 24 -- Received: from [80.122.101.100] by web110812.mail.gq1.yahoo.com via HTTP; Wed, 21 Oct 2009 07:06:08 PDT
40, 24 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
40, 24 -- References: {200910201441.n9KEfP8P010132-at-ns.microscopy.com}
40, 24 -- Date: Wed, 21 Oct 2009 07:06:08 -0700 (PDT)
40, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
40, 24 -- Subject: Re: [Microscopy] RE: E-coli in water image in SEM
40, 24 -- To: microscopy-at-microscopy.com
40, 24 -- In-Reply-To: {200910201441.n9KEfP8P010132-at-ns.microscopy.com}
40, 24 -- MIME-Version: 1.0
40, 24 -- Content-Type: text/plain; charset=iso-8859-1
40, 24 -- Content-Transfer-Encoding: 8bit
40, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LE68cN004649
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Wed, 21 Oct 2009 15:50:43 -0500
Subject: [Microscopy] Re: viaWWW: Osmium Tetroxide Waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joan
Our office of environmental safety requires that
we collect all waste (osmium, glutaraldehyde,
buffers, alcohols, resins, etc). They pick it
all up and dispose of it in whatever way is
kosher here in NYC. Nothing goes down the drain
anymore.
Lee




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org


==============================Original Headers==============================
9, 35 -- From lcgould-at-med.cornell.edu Wed Oct 21 15:50:42 2009
9, 35 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LKogF9008102
9, 35 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 15:50:42 -0500
9, 35 -- MIME-version: 1.0
9, 35 -- Content-type: text/plain; charset=iso-8859-1; format=flowed
9, 35 -- Received: from mpx4.med.cornell.edu ([140.251.11.120])
9, 35 -- by mail-gw2.med.cornell.edu
9, 35 -- (Sun Java(tm) System Messaging Server 6.3-8.03 (built Apr 24 2009; 32bit))
9, 35 -- with ESMTP id {0KRV00C3HTWHD9D0-at-mail-gw2.med.cornell.edu} for
9, 35 -- microscopy-at-microscopy.com; Wed, 21 Oct 2009 16:50:42 -0400 (EDT)
9, 35 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
9, 35 -- by mpx4.med.cornell.edu
9, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.04 64bit (built Jul 2 2009))
9, 35 -- with ESMTPA id {0KRV00LN3TWD9B70-at-mpx4.med.cornell.edu} ; Wed,
9, 35 -- 21 Oct 2009 16:50:41 -0400 (EDT)
9, 35 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379,
9, 35 -- Antispam-Data: 2009.10.21.203917
9, 35 -- X-Perlmx-Spam: Gauge=X, , Probability=10%, Report=' TO_IN_SUBJECT 0.5,
9, 35 -- BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
9, 35 -- TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0,
9, 35 -- __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0,
9, 35 -- __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
9, 35 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0,
9, 35 -- __URI_NS '
9, 35 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
9, 35 -- Message-id: {p06230907c705242abd15-at-[140.251.48.23]}
9, 35 -- In-reply-to: {200910210119.n9L1J5He003405-at-ns.microscopy.com}
9, 35 -- References: {200910210119.n9L1J5He003405-at-ns.microscopy.com}
9, 35 -- Date: Wed, 21 Oct 2009 16:50:34 -0400
9, 35 -- To: Joan.Sempf-at-va.gov, Microscopy Listserver {microscopy-at-microscopy.com}
9, 35 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
9, 35 -- Subject: Re: [Microscopy] viaWWW: Osmium Tetroxide Waste
9, 35 -- Content-Transfer-Encoding: 8bit
9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LKogF9008102
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Wed, 21 Oct 2009 16:57:47 -0500
Subject: [Microscopy] Re: viaWWW: Negative Staining for TEM Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey Ryazantsev (UCLA) is likely the most experienced person still
around in what you are asking about. He did this kind of work in the
70-80s on ribosomes. Google his name or perhaps search the MSA
archives - 3 years ago at least.
Sergey liked to speak his mind too often and so got banned from this
list (what a shame!..). He is a nice person, one really eager to share
his vast EM preparation knowledge and experience.

Vlad
________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
National Institute of Biomedical Imaging and Bioengineering, NIH
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov

Opinions and experiences related are those of Vlad Speransky and do
not represent the NIH. On the good side, this message is not
confidential and can be freely shared and reproduced.

==============================Original Headers==============================
3, 22 -- From vladislav_speransky-at-nih.gov Wed Oct 21 16:57:47 2009
3, 22 -- Received: from out5.smtp.messagingengine.com (out5.smtp.messagingengine.com [66.111.4.29])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LLviK8025083
3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 16:57:46 -0500
3, 22 -- Received: from compute2.internal (compute2.internal [10.202.2.42])
3, 22 -- by gateway1.messagingengine.com (Postfix) with ESMTP id 8A024B4DC6;
3, 22 -- Wed, 21 Oct 2009 17:57:44 -0400 (EDT)
3, 22 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161])
3, 22 -- by compute2.internal (MEProxy); Wed, 21 Oct 2009 17:57:44 -0400
3, 22 -- X-Sasl-enc: DeO28xATly7u8cmY/ortZUxaa1LbCyJiUYZMpT4FAjhy 1256162264
3, 22 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
3, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 55721B619;
3, 22 -- Wed, 21 Oct 2009 17:57:44 -0400 (EDT)
3, 22 -- Message-Id: {00BE1B45-5773-4BD9-9715-8D6D4872421B-at-nih.gov}
3, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
3, 22 -- To: Microscopy-at-microscopy.com
3, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- Subject: Re: [Microscopy] viaWWW: Negative Staining for TEM Imaging
3, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
3, 22 -- Date: Wed, 21 Oct 2009 17:57:43 -0400
3, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: glenmac-at-u.washington.edu
Date: Wed, 21 Oct 2009 18:32:28 -0500
Subject: [Microscopy] Texas Red aggregates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello
I'm looking at uptake of a drug labeled with Texas Red. Small
punctate specks of red fluorescence appear over time that excite with
a 490/40 filter and emit in the red range. They do not excite with a
Cy3 filter. Has anyone experience a spectra shift with Texas Red in
aggregates or in organelles?

Thanks,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************



==============================Original Headers==============================
7, 25 -- From glenmac-at-u.washington.edu Wed Oct 21 18:32:27 2009
7, 25 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNWRi9010527
7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:32:27 -0500
7, 25 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.7] (may be forged))
7, 25 -- by mxout4.cac.washington.edu (8.14.3+UW09.06/8.14.3+UW09.05) with ESMTP id n9LNWQgD028101
7, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK)
7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 16:32:26 -0700
7, 25 -- X-Auth-Received: from d-128-95-178-152.dhcp4.washington.edu (D-128-95-178-152.dhcp4.washington.edu [128.95.178.152])
7, 25 -- (authenticated authid=glenmac)
7, 25 -- by smtp.washington.edu (8.14.3+UW09.06/8.14.3+UW09.05) with ESMTP id n9LNWQE2013197
7, 25 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT)
7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 16:32:26 -0700
7, 25 -- From: Glen MacDonald {glenmac-at-u.washington.edu}
7, 25 -- Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
7, 25 -- Content-Transfer-Encoding: 7bit
7, 25 -- Subject: Texas Red aggregates
7, 25 -- Date: Wed, 21 Oct 2009 16:32:25 -0700
7, 25 -- Message-Id: {503AF2E4-5534-4C6D-9933-B6F998F66C88-at-u.washington.edu}
7, 25 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com}
7, 25 -- Mime-Version: 1.0 (Apple Message framework v1076)
7, 25 -- X-Mailer: Apple Mail (2.1076)
7, 25 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.21.231816
7, 25 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report='
7, 25 -- BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0, TO_NO_NAME 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MIME_VERSION_APPLEMAIL 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0'
==============================End of - Headers==============================




From: telmonunes-at-hotmail.com
Date: Wed, 21 Oct 2009 18:48:42 -0500
Subject: [Microscopy] viaWWW: Freon on JEOL JEM 200CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both telmonunes-at-hotmail.com as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: telmonunes-at-hotmail.com
Name: Telmo

Organization: Faculdade de Ciencias da Universidade de Lisboa

Title-Subject: [Filtered] Freon on JEOL JEM 200CX

Question: I work with a JEOL JEM 200CX and the
freon used as dielectric in the gun has ended, I
would like to exchange this gas to other, because
freon is forbidden to be commercialized (is
responsible for the destruction of the ozone
layer).
My question is, what gas can I use instead the freon?
In high-tension installations itís used the
Sulfur hexafluoride (SF6), but I donít know if I
can use it inside the microscope column.

Thank you.

Regards

Telmo

Login Host: 194.117.18.101
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Wed Oct 21 18:48:42 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNmeT5025483
10, 13 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:48:42 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240805c7054e3e96b6-at-[206.69.208.22]}
10, 13 -- Date: Wed, 21 Oct 2009 18:48:39 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: telmonunes-at-hotmail.com (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: Freon on JEOL JEM 200CX
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LNmeT5025483
==============================End of - Headers==============================




From: zhengji-at-mail.nih.gov
Date: Wed, 21 Oct 2009 18:49:31 -0500
Subject: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zhengji-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: zhengji-at-mail.nih.gov
Name: Jiwen Zheng

Organization: SAIC-Frederick

Title-Subject: [Filtered] TEM Sample preparation: dextran-coated Iron
oxide nanoparticle

Question: Anyone here has experience on how to prepare dextran-coated
iron oxide nanoparticle (MRI Agent) for TEM?

The protocol I followed is just dropping 3uL of nanoparticles
solution on formvar/carbon coated copper grid and then blotting off
solution after 1 min. The particles on the grid appears highly
aggregated, sometime worm-like and sometime show randomly-aggregated.
Further rinsing with DI water didn't reduce agglomerates. This is
significantly different from the AFM data which suggests most of
particles appear single particle.

Zeta potential measurement show the surface charge of this particle
is close to neutral, so the functionalized grid, such as SMART grid
(silicon grid, Dune science) doesn't help much in such case.

Any suggestions on how to minimize the artifact by optimize the grid
(surface charge, functional group, and hydrophilicity) and particle
deposition.

Thanks in advance!

Login Host: 129.43.32.241
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Wed Oct 21 18:49:31 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNnUXL027121
10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:49:31 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240806c7054e649fa7-at-[206.69.208.22]}
10, 12 -- Date: Wed, 21 Oct 2009 18:49:29 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: zhengji-at-mail.nih.gov (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: TEM Sample preparation: dextran-coated Iron oxide
10, 12 -- nanoparticle
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: avergason-at-tetracore.com
Date: Wed, 21 Oct 2009 18:50:14 -0500
Subject: [Microscopy] viaWWW: Phillips CM-10 Quad Serial lnterface Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both avergason-at-tetracore.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: avergason-at-tetracore.com
Name: Amy Vergason

Organization: Tetracore, Inc.

Title-Subject: [Filtered] Phillips CM-10 Quad Serial lnterface Board

Question: Hello Everyone,

I am looking for an old CM-10 Quad Serial Interface Board (product
number PW6440/00) used for connecting a Phillips CM-10 to a Gatan
camera. Does anyone have one of these kicking around that you would
like to sell? While I am at it, I am also looking for some old
software called "CM Remote" to be used on the same TEM.

Thanks!
Amy

Login Host: 66.3.246.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Oct 21 18:50:14 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNoDiF029511
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:50:14 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240807c7054e96ab57-at-[206.69.208.22]}
8, 11 -- Date: Wed, 21 Oct 2009 18:50:12 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: avergason-at-tetracore.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Phillips CM-10 Quad Serial lnterface Board
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ryan.walker-at-cchmc.org
Date: Wed, 21 Oct 2009 18:50:50 -0500
Subject: [Microscopy] viaWWW: Parafin Sections to TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ryan.walker-at-cchmc.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ryan.walker-at-cchmc.org
Name: Ryan Walker

Organization: CCHMC

Title-Subject: [Filtered] Parafin Sections to TEM?

Question: Hello Everyone,

I would like to take sections off a slide and embed them in resin for
TEM and am not entirely sure if this possible.

I am working with nervous tissue (Trigeminal ganglia)which have been
fixed in 2%para 0.5% glut in PBS (perfusion of the mouse) and have
dehydrated them for parafin embeddment and sectioned (approx 12um)
the tissue. In order to localize the area of interest in the ganglia
(this area is very infrequent and quite small) I utilized
immunohistochemistry with the DAB method of detection. The sections
are still attached to slides and I would like (if possible) to use
those sections for the TEM. I am able to get some of the sections
off of the slide albeit this is laborious and very difficult to see
with the naked eye. I do not need perfect ultrastructure therefore I
am not worried about the wear and tear the sections have endured from
the peroxide reaction. However, I would still like to osmicate the
tissue prior to resin embedment. Any suggestions on to process the
sections for TEM would helpful.

Do you think it would be best to just redo the expirement using
agarose embeddment and a vibratome?

Thank you all in advance and for your time!

Ryan

Login Host: 205.142.197.33
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Oct 21 18:50:50 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNolVK031483
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:50:49 -0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240808c7054ebeb4da-at-[206.69.208.22]}
11, 11 -- Date: Wed, 21 Oct 2009 18:50:46 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: ryan.walker-at-cchmc.org (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Parafin Sections to TEM?
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Wed, 21 Oct 2009 19:48:08 -0500
Subject: [Microscopy] Re: viaWWW: TEM Sample preparation: dextran-coated Iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try subjecting the grid to glow discharge (AC high voltage under
rotary pumping yields a plasma) for 30 seconds or so. This is the the
best way to make carbon coated grids less hydrophobic. Alternatively,
put your preparation on the Formvar side, rather than the carbon side.

I believe the worm like structures may be due to a drying artifact
caused by the receding water pulling your sample along the edge of the
drying droplet. Maybe if you dry the specimen faster (say at 60-80 C).
Try not blotting, but faster drying. Also, dilute your sample a bit. I
find that overly concentrated samples tend to aggregate more readily.

Let us know how it works out.



--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901

==============================Original Headers==============================
6, 18 -- From bozzola-at-siu.edu Wed Oct 21 19:48:08 2009
6, 18 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9M0m7GX020131
6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 19:48:08 -0500
6, 18 -- Received: by pzk37 with SMTP id 37so5700709pzk.10
6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 17:48:07 -0700 (PDT)
6, 18 -- MIME-Version: 1.0
6, 18 -- Received: by 10.115.101.5 with SMTP id d5mr8408675wam.23.1256172487392; Wed,
6, 18 -- 21 Oct 2009 17:48:07 -0700 (PDT)
6, 18 -- In-Reply-To: {200910212350.n9LNoCLt029473-at-ns.microscopy.com}
6, 18 -- References: {200910212350.n9LNoCLt029473-at-ns.microscopy.com}
6, 18 -- Date: Wed, 21 Oct 2009 19:48:07 -0500
6, 18 -- Message-ID: {ebc2299e0910211748s58c763c7xfedd224593765e6f-at-mail.gmail.com}
6, 18 -- Subject: Re: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron
6, 18 -- oxide
6, 18 -- From: John Bozzola {bozzola-at-siu.edu}
6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com}
6, 18 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: ron.doole-at-materials.ox.ac.uk
Date: Thu, 22 Oct 2009 02:02:02 -0500
Subject: [Microscopy] viaWWW: Freon on JEOL JEM 200CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Telmo,

Some years ago we changed the Freon gas in our JEOL TEMs, including a 200CX, from Freon to SF6. Apart from changing the gas inlet fittings to allow us to use a standard SF6 gas cylinder instead of the JEOL cans we made no other changes. We pumped out the Freon from both the tank and the gun, filled them with air and then pumped out again, we then filled them with SF6 to the same pressure as the Freon (tank 0.15kg/cm, gun 3.0 kg/cm2). The 200CX ran happily until we scrapped it about 7 years later, the other instruments are still running at 200 or 400kV.


regards,
Ron

Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: telmonunes-at-hotmail.com [telmonunes-at-hotmail.com]
Sent: 22 October 2009 01:02
To: Ron Doole

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both telmonunes-at-hotmail.com as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: telmonunes-at-hotmail.com
Name: Telmo

Organization: Faculdade de Ciencias da Universidade de Lisboa

Title-Subject: [Filtered] Freon on JEOL JEM 200CX

Question: I work with a JEOL JEM 200CX and the
freon used as dielectric in the gun has ended, I
would like to exchange this gas to other, because
freon is forbidden to be commercialized (is
responsible for the destruction of the ozone
layer).
My question is, what gas can I use instead the freon?
In high-tension installations itís used the
Sulfur hexafluoride (SF6), but I donít know if I
can use it inside the microscope column.

Thank you.

Regards

Telmo

Login Host: 194.117.18.101
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Wed Oct 21 18:48:42 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNmeT5025483
10, 13 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:48:42 -0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240805c7054e3e96b6-at-[206.69.208.22]}
10, 13 -- Date: Wed, 21 Oct 2009 18:48:39 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: telmonunes-at-hotmail.com (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: Freon on JEOL JEM 200CX
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9LNmeT5025483
==============================End of - Headers==============================

==============================Original Headers==============================
17, 30 -- From ron.doole-at-materials.ox.ac.uk Thu Oct 22 02:02:01 2009
17, 30 -- Received: from relay3.mail.ox.ac.uk (relay3.mail.ox.ac.uk [163.1.2.165])
17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9M721hV021343
17, 30 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 02:02:01 -0500
17, 30 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk)
17, 30 -- by relay3.mail.ox.ac.uk with esmtp (Exim 4.69)
17, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
17, 30 -- id 1N0rgO-0007Te-BU; Thu, 22 Oct 2009 08:02:00 +0100
17, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.2.156]) by
17, 30 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Thu, 22 Oct 2009 08:02:00
17, 30 -- +0100
17, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
17, 30 -- To: "telmonunes-at-hotmail.com" {telmonunes-at-hotmail.com}
17, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
17, 30 -- Date: Thu, 22 Oct 2009 08:01:59 +0100
17, 30 -- Subject: RE: [Microscopy] viaWWW: Freon on JEOL JEM 200CX
17, 30 -- Thread-Topic: [Microscopy] viaWWW: Freon on JEOL JEM 200CX
17, 30 -- Thread-Index: AcpSqu8vorgKxf10TASYqtT0ijI8TgAOLg2l
17, 30 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF9264C21C46F-at-EXMBX02.ad.oak.ox.ac.uk}
17, 30 -- References: {200910220002.n9M02MFk010864-at-ns.microscopy.com}
17, 30 -- In-Reply-To: {200910220002.n9M02MFk010864-at-ns.microscopy.com}
17, 30 -- Accept-Language: en-US, en-GB
17, 30 -- Content-Language: en-GB
17, 30 -- X-MS-Has-Attach:
17, 30 -- X-MS-TNEF-Correlator:
17, 30 -- acceptlanguage: en-US, en-GB
17, 30 -- Content-Type: text/plain; charset="iso-8859-1"
17, 30 -- MIME-Version: 1.0
17, 30 -- Content-Transfer-Encoding: 8bit
17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9M721hV021343
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 22 Oct 2009 02:58:22 -0500
Subject: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jiwen!

The preparation of fine particles for TEM and their aggregation is a regular question on the list. Some search of the archives could really help.
Once, after I gave some advices, the person was kind enough to send my an answer telling that it worked.

1) Dilute the particles
2) Use volatile solvent like ethanol/methanol/acetone instead of water (organic solvents may destroy the formvar film, better use carbon grid without formvar)
3) ultrasonicate just before blotting
4) You may wish to slowly blow on the grid after blotting to speed up drying

It is often the case that particles aggregate due to electrostatic interactions. In this case, adding NaCl in the solution would decrease the electrostatic interactions between the particles. Of course the salt will dry on the grid too. Perhaps you can wash it carefully with water without washing the particles out, but I have to admit that is risky. Sort of last chance strategy ;-)

Best regards,
Stephane




----- Original Message ----
X-from: "zhengji-at-mail.nih.gov" {zhengji-at-mail.nih.gov}
To: nizets2-at-yahoo.com
Sent: Thu, October 22, 2009 1:55:12 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both zhengji-at-mail.nih.gov as well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: zhengji-at-mail.nih.gov
Name: Jiwen Zheng

Organization: SAIC-Frederick

Title-Subject: [Filtered] TEM Sample preparation: dextran-coated Iron
oxide nanoparticle

Question: Anyone here has experience on how to prepare dextran-coated
iron oxide nanoparticle (MRI Agent) for TEM?

The protocol I followed is just dropping 3uL of nanoparticles
solution on formvar/carbon coated copper grid and then blotting off
solution after 1 min. The particles on the grid appears highly
aggregated, sometime worm-like and sometime show randomly-aggregated.
Further rinsing with DI water didn't reduce agglomerates. This is
significantly different from the AFM data which suggests most of
particles appear single particle.

Zeta potential measurement show the surface charge of this particle
is close to neutral, so the functionalized grid, such as SMART grid
(silicon grid, Dune science) doesn't help much in such case.

Any suggestions on how to minimize the artifact by optimize the grid
(surface charge, functional group, and hydrophilicity) and particle
deposition.

Thanks in advance!

  Login Host: 129.43.32.241
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Wed Oct 21 18:49:31 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNnUXL027121
10, 12 --     for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:49:31 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240806c7054e649fa7-at-[206.69.208.22]}
10, 12 -- Date: Wed, 21 Oct 2009 18:49:29 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: zhengji-at-mail.nih.gov (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: TEM Sample preparation: dextran-coated Iron oxide
10, 12 --  nanoparticle
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
28, 25 -- From nizets2-at-yahoo.com Thu Oct 22 02:58:22 2009
28, 25 -- Received: from web110810.mail.gq1.yahoo.com (web110810.mail.gq1.yahoo.com [67.195.13.233])
28, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9M7wMR9005573
28, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 02:58:22 -0500
28, 25 -- Received: (qmail 20635 invoked by uid 60001); 22 Oct 2009 07:58:21 -0000
28, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1256198301; bh=cmKrxnpatxC3drkg2oeADwJiSEpddo9Yj+/aN80ASr4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=XXvcVqeySg26AiOIFMM41xl4n/SGQkRAvZhxRa0nGclLCJCRHv+Yd/L90KoKE5q/qs5a3bENVNW7jlvM2oMCU5zEaNolj2YARghYd2XkmiWP/6oAJj7sCpJ5e1O3KeYNQBqjQyu5KFvF8+O2sYXPsPVPV6U3shcsV/UveO55y5c=
28, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
28, 25 -- s=s1024; d=yahoo.com;
28, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
28, 25 -- b=eVpuyZApd3XcG4ZG7v7PL7U3v7mEsLoht4RiV7yHh1CL+KX8ZnWg3uVChrF99C+SV5tgsKi7EDqmjyC065E03XZN5R+SiLLuAacBj+VzlynulqqlwF/JwAIskk8J+8V6mVcwMtIPU72O6f5TatTfBZaEoV7tnP20xVCBMVLrCqs=;
28, 25 -- Message-ID: {683001.19982.qm-at-web110810.mail.gq1.yahoo.com}
28, 25 -- X-YMail-OSG: 6TLgNv4VM1m4DFjIM42XkFC61WQdhAQP_FQ3eHyOCGCbWx9KixdohZGitAFYvc1ucz.k344DcYroIkNB5Mc5uQUEs_iHpV6_VnilHQabNV2I9v_vT_DlqCKVRYHUzbxhqsRnl1u11WeJd9B4mytU_3oFyQ1kBF3vTt55HbGrK4MQeg1xnwjaEe33yca2W.Nf7jxDvoYr5_fW7Ek2CbzHODqa.XDDplLn.OrJf2msiRLnOfZCtKESo.YqG1bl2u1QX68TtxBP9i.IsCPTZHsQ6MU9TkKIEyo_FNOQKfUKo.c15hlTGMtslfmIy30UdCX4FTY7ec63yLyGyhhY.NQeNKCW3.dq_AtVFy8s0KZJukaYAudZn5CblyPmsw--
28, 25 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Thu, 22 Oct 2009 00:58:21 PDT
28, 25 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
28, 25 -- References: {200910212355.n9LNtCGZ015665-at-ns.microscopy.com}
28, 25 -- Date: Thu, 22 Oct 2009 00:58:21 -0700 (PDT)
28, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
28, 25 -- Subject: Re: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron oxide
28, 25 -- To: zhengji-at-mail.nih.gov
28, 25 -- Cc: microscopy-at-microscopy.com
28, 25 -- In-Reply-To: {200910212355.n9LNtCGZ015665-at-ns.microscopy.com}
28, 25 -- MIME-Version: 1.0
28, 25 -- Content-Type: text/plain; charset=iso-8859-1
28, 25 -- Content-Transfer-Encoding: 8bit
28, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9M7wMR9005573
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 22 Oct 2009 03:07:08 -0500
Subject: [Microscopy] Quantomix wetsem capsules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What about the use of Helium SEM with these capsules?
Would it not be more appropriate than electrons in this special case?
Perhaps it would even be possible to use secondary electrons?
I am not really a specialist of He SEMs, so my question is really open.

All this new technology is really exciting!

Stephane




==============================Original Headers==============================
5, 22 -- From nizets2-at-yahoo.com Thu Oct 22 03:07:08 2009
5, 22 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9M878Pv020235
5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 03:07:08 -0500
5, 22 -- Received: (qmail 12324 invoked by uid 60001); 22 Oct 2009 08:07:07 -0000
5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1256198827; bh=Q4m3IsfVpsLqRtX+k+gHHhBsKTzSTqmf2aO++NZSYzA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type; b=1y7p2yxEr9e/CDGIxdoGkppBJ7egOmaTVZziL9m0wtZ4mR3hz73K3B0Ctowkk5PO1DV0SnRI+foeHpYiNPOAj1dEom/uHLnO0HRp/BLSZU3ofkXBfF3bY3sg7GCpe3pcqu26wEk9FHd1NA0Kh9DceXCMWVL8qX6syhdhIQQ6KJ4=
5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 22 -- s=s1024; d=yahoo.com;
5, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type;
5, 22 -- b=vwNgDN7/gkI5foKF6nyLZW5iG6wa55U7/sd04AtcgoLotNTzlwSgSS2IwkfqdHQjNhdco8Puo9/qjUgxz+rul4Q0fJwuaNoGL1arC3oSqNzQ8dkoTjx/nqOJTZIU4qgZw9VkEB2HnVQbiTY/sO0Bu3DTNPs/EfCTFh+Ab0j/XTQ=;
5, 22 -- Message-ID: {580958.11333.qm-at-web110814.mail.gq1.yahoo.com}
5, 22 -- X-YMail-OSG: rXEOuLcVM1lMFrMEYMIb.B4LDiV464PIkKRh7X62EN.32w0G5JTifubD4W5YTH7b7EfCWxpoyaXaoxW3XUEha2uamF9sJbDNTRZBxe1Oe3cq_dG7nZVBSm4s9ZcZT1WAdEUxPJCDpJrh70d9JQQQgpmgOMZjI3oKTfgGFlCnvGMSrrmn2DHJHAt6Nw4fZ4RDe4egOg6hYMwjyMicc2P5DwV8dmpvTximd8lPoQW0_c61CItNqKojsaz.7XRPG6KQeMpgcTxyh5ZXDo5_iPIVfDvEVqi036TZrpJwtTBPceNyNfge6S9VHQRoD59gfHMlR5Yy0NLYrsnY373OsfTa1Kk-
5, 22 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Thu, 22 Oct 2009 01:07:07 PDT
5, 22 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
5, 22 -- References: {200910211409.n9LE9SRx010928-at-ns.microscopy.com}
5, 22 -- Date: Thu, 22 Oct 2009 01:07:07 -0700 (PDT)
5, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 22 -- Subject: Quantomix wetsem capsules
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- In-Reply-To: {200910211409.n9LE9SRx010928-at-ns.microscopy.com}
5, 22 -- MIME-Version: 1.0
5, 22 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Thu, 22 Oct 2009 07:35:19 -0500
Subject: [Microscopy] EBSD energy filter available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EBSD energy filter

I have an energy filter that permits obtaining EBSD patterns with only
those electrons having an energy above a chosen cut off energy. Work
using this filter has been the basis of several publications. The work
is summarized in chapter 4 of the second edition of Electron
Backscatter Diffraction in Materials Science by Schwartz et al.

For various reasons, the filter will not be used again here at Lehigh.
Will anyone who would have a use for it please contact me. Note that
the filter requires a rather large port on the SEM; we used it on an FEI
Dual-Beam FIB.

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
6, 21 -- From jae5-at-lehigh.edu Thu Oct 22 07:35:18 2009
6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MCZIn6016378
6, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 07:35:18 -0500
6, 21 -- Received: from [127.0.0.1] (Dyn055026.mat.Lehigh.EDU [128.180.55.26])
6, 21 -- (authenticated bits=0)
6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n9MCZIGb002672
6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 08:35:18 -0400
6, 21 -- Message-ID: {4AE05188.8010603-at-lehigh.edu}
6, 21 -- Date: Thu, 22 Oct 2009 08:35:20 -0400
6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu}
6, 21 -- Organization: Lehigh University
6, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 21 -- MIME-Version: 1.0
6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
6, 21 -- Subject: EBSD energy filter available
6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- X-Virus-Scanned: clamav-milter 0.95.2 at rain.cc.lehigh.edu
6, 21 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Thu, 22 Oct 2009 07:54:35 -0500
Subject: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Jiwen,

I've had the best success with these materials using a combination of some of the suggestions already posted. You're correct, the samples consist of single nanoparticles, but aggregation during drying on the grid is an issue. I use copper grids coated with carbon film, no formvar. The samples I've worked with have been either aqueous suspensions or dried solids. I use distilled water to either dilute the liquid or re-suspend the solid, and I dilute until I have a suspension with a faint orange color. I put one drop of this onto a grid and wick away most of the excess liquid, allowing the remaining thin film to air dry. If the liquid is still somewhat viscous after dilution and coalesces on the grid while drying, it can help to gently drag the drop across the grid film a couple of times, using a the tip of a fine pair of forceps or an eyelash brush. This will help to deposit the particles across the film.

While some samples will disperse nicely as single particles or small aggregates, most times I find a mix of areas with dispersed and aggregated particles. The dispersed particles will be best for imaging, but you'll want to examine the larger aggregates also, to ensure that you've sampled representatively, and that there hasn't been size segregation as the film has dried across the grid.

Hope this helps.

Regards,

Elaine


*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com



*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
X-from: zhengji-at-mail.nih.gov [mailto:zhengji-at-mail.nih.gov]
Sent: Wednesday, October 21, 2009 7:03 PM
To: Elaine F. Schumacher

This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zhengji-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: zhengji-at-mail.nih.gov
Name: Jiwen Zheng

Organization: SAIC-Frederick

Title-Subject: [Filtered] TEM Sample preparation: dextran-coated Iron oxide nanoparticle

Question: Anyone here has experience on how to prepare dextran-coated iron oxide nanoparticle (MRI Agent) for TEM?

The protocol I followed is just dropping 3uL of nanoparticles solution on formvar/carbon coated copper grid and then blotting off solution after 1 min. The particles on the grid appears highly aggregated, sometime worm-like and sometime show randomly-aggregated.
Further rinsing with DI water didn't reduce agglomerates. This is significantly different from the AFM data which suggests most of particles appear single particle.

Zeta potential measurement show the surface charge of this particle is close to neutral, so the functionalized grid, such as SMART grid (silicon grid, Dune science) doesn't help much in such case.

Any suggestions on how to minimize the artifact by optimize the grid (surface charge, functional group, and hydrophilicity) and particle deposition.

Thanks in advance!

Login Host: 129.43.32.241
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Wed Oct 21 18:49:31 2009 10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9LNnUXL027121
10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:49:31 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240806c7054e649fa7-at-[206.69.208.22]}
10, 12 -- Date: Wed, 21 Oct 2009 18:49:29 -0500 10, 12 -- To: microscopy-at-microscopy.com 10, 12 -- From: zhengji-at-mail.nih.gov (by way of MicroscopyListserver) 10, 12 -- Subject: viaWWW: TEM Sample preparation: dextran-coated Iron oxide 10, 12 -- nanoparticle 10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
28, 29 -- From eschumacher-at-mccrone.com Thu Oct 22 07:54:35 2009
28, 29 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MCsYUI031847
28, 29 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 07:54:34 -0500
28, 29 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by
28, 29 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Thu, 22 Oct 2009 07:54:29
28, 29 -- -0500
28, 29 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
28, 29 -- To: "zhengji-at-mail.nih.gov" {zhengji-at-mail.nih.gov} ,
28, 29 -- "microscopy-at-microscopy.com"
28, 29 -- {microscopy-at-microscopy.com}
28, 29 -- Date: Thu, 22 Oct 2009 07:54:30 -0500
28, 29 -- Subject: RE: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated
28, 29 -- Iron oxide
28, 29 -- Thread-Topic: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated
28, 29 -- Iron oxide
28, 29 -- Thread-Index: AcpSqvt1hF5B0vfcRGCTs+MADfEGCQAakFaw
28, 29 -- Message-ID: {874B1DB532886444A60A015EE363493F0B760E41CC-at-mccronemsg07.tmg.mccrone.com}
28, 29 -- References: {200910220002.n9M02gfn012223-at-ns.microscopy.com}
28, 29 -- In-Reply-To: {200910220002.n9M02gfn012223-at-ns.microscopy.com}
28, 29 -- Accept-Language: en-US
28, 29 -- Content-Language: en-US
28, 29 -- X-MS-Has-Attach:
28, 29 -- X-MS-TNEF-Correlator:
28, 29 -- acceptlanguage: en-US
28, 29 -- Content-Type: text/plain; charset="us-ascii"
28, 29 -- MIME-Version: 1.0
28, 29 -- Content-Transfer-Encoding: 8bit
28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9MCsYUI031847
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Thu, 22 Oct 2009 08:16:16 -0500
Subject: [Microscopy] Re: viaWWW: Osmium Tetroxide Waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joan et al

sorry to be a bit late jumping in. As with Leona, our safety people
handle chemical waste. We collect all waste osmium and store it under
vegetable oil, and periodically take it down to them. I also collect
all cacodylate buffer and other possible environmental contaminants for
disposal. Paper work is horrendous. The main reason I stopped using
radioactives years ago was the paper work, not my concern for safety.

Unfortunately, we collect the Uranyl solutions but at last inquiry my
University's safety people still had no plan in place for disposal even
after my asking about this matter for about 8 years. I must admit the
last inquiry was about a year ago, maybe something is finally in place.

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 20 -- From paul_hazelton-at-umanitoba.ca Thu Oct 22 08:16:15 2009
6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MDGF2P015159
6, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 08:16:15 -0500
6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 20 -- (authenticated bits=0)
6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n9MDGEYn021659;
6, 20 -- Thu, 22 Oct 2009 08:16:14 -0500 (CDT)
6, 20 -- Message-ID: {4AE05B1A.7010109-at-umanitoba.ca}
6, 20 -- Date: Thu, 22 Oct 2009 08:16:10 -0500
6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: Joan.Sempf-at-va.gov, Microscopy Listserver {microscopy-at-microscopy.com}
6, 20 -- Subject: Re: [Microscopy] viaWWW: Osmium Tetroxide Waste
6, 20 -- References: {200910210105.n9L15uCr024210-at-ns.microscopy.com}
6, 20 -- In-Reply-To: {200910210105.n9L15uCr024210-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 22 Oct 2009 09:54:19 -0500
Subject: [Microscopy] E-coli in water image in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,
The cover material on the capsule is way too thick for secondary electrons
to be able to penetrate, therefore you are limited to backscattered
electrons with their limitations.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Wednesday, October 21, 2009 10:08 AM
To: kenconverse-at-qualityimages.biz

Would it be possible to contrast the bacteria samples with osmium tetroxyde?

I would like to use the opportunity to ask a question about the QuantomiX
WETSEM technology capsules.
Imaging eucaryotic cells in SEM is a real problem mainly due to the
crumbling of the cells under vacuum.
Would it be realistic to image cells in buffer using these technology
advanced-very expensive capsules in "near-life conditions" after fixation
and osmium tetroxide contrasting? Would it be possible to image them in SE
modus? Why not?

Regards,

Stephane



----- Original Message ----
X-from: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
To: nizets2-at-yahoo.com
Sent: Tue, October 20, 2009 4:41:25 PM

Hi Roy,

In my limited experience with these capsules I always had to add something
to enhance contrast since the actual imaging is done with backscattered
electrons.  Unstained bacteria just don't have the Z-contrast to stand out
from their surroundings.  I generally used uranyl acetate to stain bacteria,
but other heavy metal stains might work as well.  The nice thing about the
capsules is that you can view the samples first to see what is needed, then
add contrasting agent, view the same sample again, take them out and add
more stain, if necessary, and continue viewing.

Poor contrast is definitely a problem with this system.  Even after using
UA, we often had to strongly enhance contrast with imaging programs.  This,
of course, should always be noted when presenting the images.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week
&NavType=Both&Type=TimePlan
Sons of Norway:  http://www.sofn.com




-----Original Message-----
X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu]
Sent: Monday, October 19, 2009 5:08 PM
To: Tindall, Randy D.

Group,

Working on a project outside my experience and could use some help.

Trying to image E-coli in water samples using the QuantomiX WETSEM
technology capsules.

The calibration sample from Quantomix works fine with backscatter image
showing 500nm and 40nm particles.

Tried raw water sample and had no luck seeing anything. Pretty sure I need
to add some contrasting media to water sample but not sure what to add or if
that will work. Suggestions from some of you bio prep folks would be greatly
appreciated.

Thanks

Roy Beavers
Southern Methodist University
Department of Earth Sciences
P.O. Box 750395
Dallas, TX  75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



==============================Original Headers==============================
9, 27 -- From rbeavers-at-mail.smu.edu Mon Oct 19 17:07:34 2009
9, 27 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu
[129.119.65.151])
9, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9JM7X3i012654
9, 27 --     for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 17:07:34
-0500
9, 27 -- X-IronPort-Anti-Spam-Filtered: true
9, 27 -- X-IronPort-Anti-Spam-Result:
ApoEAFuA3EqBd0GE/2dsb2JhbADMTQEJhUSITIJOgWME
9, 27 -- Received: from sxht1p1.systems.smu.edu ([129.119.65.132])
9, 27 --  by smtah2.systems.smu.edu with ESMTP/TLS/RC4-MD5; 19 Oct 2009
17:07:34 -0500
9, 27 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by
9, 27 --  sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 19 Oct
2009
9, 27 --  17:07:31 -0500
9, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu}
9, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
9, 27 -- Date: Mon, 19 Oct 2009 17:07:30 -0500
9, 27 -- Subject: E-coli in water image in SEM
9, 27 -- Thread-Topic: E-coli in water image in SEM
9, 27 -- Thread-Index: AcpRCI1+dDLCzvjFSVqSLN7yTeUgHQ==
9, 27 -- Message-ID:
{7A6FE75608A3624E872147993C8B36BB7E3EA011D5-at-SXMBXC.systems.smu.edu}
9, 27 -- Accept-Language: en-US
9, 27 -- Content-Language: en-US
9, 27 -- X-MS-Has-Attach:
9, 27 -- X-MS-TNEF-Correlator:
9, 27 -- acceptlanguage: en-US
9, 27 -- Content-Type: text/plain; charset="iso-8859-1"
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-Transfer-Encoding: 8bit
9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9JM7X3i012654
==============================End of - Headers==============================


==============================Original Headers==============================
23, 33 -- From TindallR-at-missouri.edu Tue Oct 20 09:36:28 2009
23, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
(mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
23, 33 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9KEaRjf001231
23, 33 --     for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2009 09:36:27
-0500
23, 33 -- X-IronPort-Anti-Spam-Filtered: true
23, 33 -- X-IronPort-Anti-Spam-Result:
ApoEALPi1krRauUo/2dsb2JhbADLJgEJhmaISYJOgWIEgVuJEw
23, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
([209.106.229.40])
23, 33 --  by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Oct 2009
09:36:27 -0500
23, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
23, 33 --     Tue, 20 Oct 2009 09:36:27 -0500
23, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.222]) by
23, 33 --  UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Tue, 20
Oct 2009
23, 33 --  09:36:22 -0500
23, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 33 -- To: "'rbeavers-at-mail.smu.edu'" {rbeavers-at-mail.smu.edu}
23, 33 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
23, 33 -- Date: Tue, 20 Oct 2009 09:36:22 -0500
23, 33 -- Subject: RE: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Topic: [Microscopy] E-coli in water image in SEM
23, 33 -- Thread-Index: AcpRCKmJBPCdwfqPTsK18bjNEK6C1AAiSalQ
23, 33 -- Message-ID:
{9422E68616A7C648A281C0B5CD22A4B81299905642-at-UM-EMAIL06.um.umsystem.edu}
23, 33 -- References: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- In-Reply-To: {200910192208.n9JM8G3x014777-at-ns.microscopy.com}
23, 33 -- Accept-Language: en-US
23, 33 -- Content-Language: en-US
23, 33 -- X-MS-Has-Attach:
23, 33 -- X-MS-TNEF-Correlator:
23, 33 -- acceptlanguage: en-US
23, 33 -- Content-Type: text/plain; charset="iso-8859-1"
23, 33 -- MIME-Version: 1.0
23, 33 -- X-OriginalArrivalTime: 20 Oct 2009 14:36:27.0021 (UTC)
FILETIME=[B494DBD0:01CA5192]
23, 33 -- Content-Transfer-Encoding: 8bit
23, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9KEaRjf001231
==============================End of - Headers==============================






==============================Original Headers==============================
40, 24 -- From nizets2-at-yahoo.com Wed Oct 21 09:06:09 2009
40, 24 -- Received: from web110812.mail.gq1.yahoo.com
(web110812.mail.gq1.yahoo.com [67.195.13.235])
40, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n9LE68cN004649
40, 24 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 09:06:09
-0500
40, 24 -- Received: (qmail 842 invoked by uid 60001); 21 Oct 2009 14:06:08
-0000
40, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1256133968; bh=vIJJ3ISVyC83CS7Z9JmH+36ksaQiEaPbXfaPknxFkRE=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:I
n-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=Jdnq5RA80FNxgXc51nZWXyRnErc3S1AWqOg4qCScJcM3H7R54XXdKFlcT741T24fRB//8DZZ35
uTl5FRpCObwys5YZ1RrvBF7Qoq2n4LjASQbFh5F64bXbw+bWlA2X3vzsCJVKjSKT0it0l7T+pTjX
Z+cq9D/V8n4eVsOSlr15o=
40, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
40, 24 -- s=s1024; d=yahoo.com;
40, 24 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:I
n-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
40, 24 --
b=FQg3PsHit41iRi+9jIJkNc7ZS3AGv/pQaGqnI4hpZs45xhmwso+RcStvNatM+aR7rAn1G1g7Di
QqJ0JOzthWCfI7UjI8Vza2YGrzSOhlq0CGWvYXXWE4e4qgImNbdBqyBSSugXR7kHsl7ryVWULCep
z1yL2ZX3xCOcLPtqGXjE4=;
40, 24 -- Message-ID: {371324.99658.qm-at-web110812.mail.gq1.yahoo.com}
40, 24 -- X-YMail-OSG:
7qryGP0VM1kVR7WF_BXqC7yZJgVsjmo9nvwrW05QSe.eSC0jvkBjD_OnGz7usSKoFbg8VeK8bT.y
tZc4WwRw4YhTcUklfa7BvxZqhsd5U8_CFwr_E7Vj3Z9KnCoHB_2KR5WEc9TG6S1ug8GrhKIBMN9z
g5d3KSQFP4tVTp35NMInBxHPoxt9dGdJxivpEr8tU_DPCdUzVMgC1Km5WDHVTbzzBjACD18gImYC
ccILi.dKa8MkwQZG08r2rmSggoAHw_pZIHmnsXD2GjJqezRrWzxI9nDeKOhTnQnd2NIusrHzWYIQ
cxVNG3B9oW5_pOFFLEZqEvaxd93ztVyRFDt60IMDYQuMsliXArzfsa3Y_I4wMruq9v4POWS9qQ--
40, 24 -- Received: from [80.122.101.100] by web110812.mail.gq1.yahoo.com
via HTTP; Wed, 21 Oct 2009 07:06:08 PDT
40, 24 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
40, 24 -- References: {200910201441.n9KEfP8P010132-at-ns.microscopy.com}
40, 24 -- Date: Wed, 21 Oct 2009 07:06:08 -0700 (PDT)
40, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
40, 24 -- Subject: Re: [Microscopy] RE: E-coli in water image in SEM
40, 24 -- To: microscopy-at-microscopy.com
40, 24 -- In-Reply-To: {200910201441.n9KEfP8P010132-at-ns.microscopy.com}
40, 24 -- MIME-Version: 1.0
40, 24 -- Content-Type: text/plain; charset=iso-8859-1
40, 24 -- Content-Transfer-Encoding: 8bit
40, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9LE68cN004649
==============================End of - Headers==============================




==============================Original Headers==============================
52, 25 -- From kenconverse-at-qualityimages.biz Thu Oct 22 09:54:19 2009
52, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
52, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MEsJkF002437
52, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 09:54:19 -0500
52, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
52, 25 -- with ESMTP
52, 25 -- id {20091022145417109.BOMX12118-at-cdptpa-omta02.mail.rr.com} ;
52, 25 -- Thu, 22 Oct 2009 14:54:17 +0000
52, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
52, 25 -- To: {nizets2-at-yahoo.com} , "MSA Listserver" {microscopy-at-microscopy.com}
52, 25 -- Subject: RE: [Microscopy] E-coli in water image in SEM
52, 25 -- Date: Thu, 22 Oct 2009 10:54:06 -0400
52, 25 -- Message-ID: {D96D49B4E073405EBFCCBBDC770A9377-at-Ken}
52, 25 -- MIME-Version: 1.0
52, 25 -- Content-Type: text/plain;
52, 25 -- charset="iso-8859-1"
52, 25 -- X-Priority: 3 (Normal)
52, 25 -- X-MSMail-Priority: Normal
52, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
52, 25 -- In-Reply-To: {200910211408.n9LE8Fas008486-at-ns.microscopy.com}
52, 25 -- Importance: Normal
52, 25 -- Thread-Index: AcpSV/DbEkpD/w4KRk6G50Xshhe17QAzw8/g
52, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
52, 25 -- Content-Transfer-Encoding: 8bit
52, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9MEsJkF002437
==============================End of - Headers==============================




From: vishnu.mogili-at-gmail.com
Date: Thu, 22 Oct 2009 10:10:43 -0500
Subject: [Microscopy] TEM Probe size in CBED mode

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

Can anyone tell me how we can measure probe size of
Jeol 2010 TEM having LaB6 filament working under CBED mode?

I want to measure strain between 10nm thick Silicon-Germanium and
Silicon layers. So I think I should use smallest available probe to
acheive it.


Thanks,

Vishnu Mogili

Materials & Surface Science Institute
University of Limerick
Ireland

==============================Original Headers==============================
7, 29 -- From vishnu.mogili-at-gmail.com Thu Oct 22 10:10:43 2009
7, 29 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.156])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MFAg9W017674
7, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 22 Oct 2009 10:10:43 -0500
7, 29 -- Received: by fg-out-1718.google.com with SMTP id e21so3422992fga.0
7, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 22 Oct 2009 08:10:42 -0700 (PDT)
7, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 29 -- d=gmail.com; s=gamma;
7, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
7, 29 -- :from:to:content-type;
7, 29 -- bh=iHDK3+ORK3b5k4eWunJwIpSFqSVDVHMDOjViT6J8TtI=;
7, 29 -- b=GyqcfilfcVWCdMrSrhWbHXbDAXgi5B1sJFcWfoClrXf5mz4ct6BYkbazHxp2xXq1EW
7, 29 -- 3Dws7BMa8tTiqZt4FK8TyZVkOzHoEFpzP1vMv3KK+INCqFXk7gKKhv4FkKB4DbRn2MoY
7, 29 -- 40Z/wl95N6e1uGxnedo3Xj40gldpP0lkcHv3Y=
7, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 29 -- d=gmail.com; s=gamma;
7, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
7, 29 -- b=a18jZ8HviIi3Nc2X8N78hxjcL13f/PB/AGNPy5bFLdaD5DfvQTwLWmao69zWacYNTp
7, 29 -- z56dp331cUDeYV6a+F7qGFrhdRBCpjHo7qhpfFjLXQZdLUwWI4JdjMPsqUOyRHtYflhb
7, 29 -- 3ywzM+UWAiRWpmfgjQqUJ1PS+q5hcLk7ootUg=
7, 29 -- MIME-Version: 1.0
7, 29 -- Received: by 10.86.240.9 with SMTP id n9mr5066645fgh.70.1256224241956; Thu, 22
7, 29 -- Oct 2009 08:10:41 -0700 (PDT)
7, 29 -- Date: Thu, 22 Oct 2009 16:10:41 +0100
7, 29 -- Message-ID: {95c1e3310910220810g7d407f52o8971d50064cf063b-at-mail.gmail.com}
7, 29 -- Subject: TEM Probe size in CBED mode
7, 29 -- From: vishnu mogili {vishnu.mogili-at-gmail.com}
7, 29 -- To: Microscopy-at-Microscopy.Com
7, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: dcromey-at-email.arizona.edu
Date: Thu, 22 Oct 2009 10:21:19 -0500
Subject: [Microscopy] viaWWW: Parafin Sections to TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ryan,

Years ago I worked in a clinical EM lab and we occasionally were called on
to do this sort of thing with paraffin sections. We used what we called the
"pop off" technique. We learned it from someone else I'm sure, but I don't
know the original source. Here's a rough outline (note, due to the thinness
of the sample, many of the dehydration steps can be done for a shorter
period of time than what you would use for a block of tissue):

* Remove the slide's coverslip.
* Cut the tip off of a BEEM capsule. Pull off the cap and discard.
* Superglue the cap end (the end you did not cut) of the BEEM capsule over
your area of interest.
* Make sure the glue has made a good seal around the end of the BEEM
capsule, add more if needed (toothpick works well). This needs to be liquid
tight.
* Once the glue is dry, use the capsule and rehydrate the tissue through a
graded series of ETOH.
(I am assuming that the section was already de-paraffinized).
* Osmicate.
* Dehydrate, infiltrate with the resin of your choice (we used SPURRS, so
there was no transitional solvent) and polymerize all in the BEEM capsule.

Now comes the tricky part. You need to create a temperature differential
between the microscope slide and the block. Some people advocate the use of
liquid nitrogen at this point, but in our hands this always ended up with a
blockface that had embedded chunks of glass that had been torn out of the
surface of the microscope slide. My coworker (Chuck) came up with an idea
that worked much better (for us). You need a hotplate and two pairs of
pliers. Place the slide, with the BEEM capsule "up" on an ~ 80 degree C
hotplate for about a minute. All the pliers work has to happen fairly
quickly (note, the slide/BEEM will be quite hot). With Pliers #1 grab the
BEEM capsule as close to the junction of BEEM and slide as possible. Use
Pliers #2 with the jaws on the slide as near to the base of the BEEM capsule
as possible. Twist Pliers #1 to the side to pop the attached surface of the
BEEM capsule off of the slide. I encourage you to practice this on a few
blanks to perfect the technique.

Once you have the block removed, you are ready to section. Remember that
you only have 4-5 microns of sample thickness to work with. With your DAB
staining you might be able to locate our area of interest so that you can
trim down the blockface. If you are having problems finding what you need,
try the time-honored technique of "blockface huffing". To "huff", blow a
short breath (say HUH) with the blockface next to your mouth and then
immediately look at the block surface under the stereoscope, and you should
see the structure in the section quite well (move the angle around for best
viewing). Note, huffing is a bit harder to accomplish when the humidity is
low.

My boss in the EM lab (Dr. Claire Payne) used to show the medical residents
a picture of an old rusted out, junkyard car. She would point out that you
could recognize it as a car, but that the more perishable parts had degraded
(in the picture) too much to determine the paint color, the type of tires,
etc. Going from paraffin to TEM involves loss of fine structure. You will
still be able to learn something, but what are you missing?

You should probably talk this technique over with the person that runs your
EM facility.

Good luck,
Doug


^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA

office: AHSC 4212 email: Cromey-at-Arizona.edu
voice: 520-626-2824 fax: 520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"

-----Original Message-----
X-from: ryan.walker-at-cchmc.org [mailto:ryan.walker-at-cchmc.org]
Sent: Wednesday, October 21, 2009 4:52 PM
To: dcromey-at-email.arizona.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ryan.walker-at-cchmc.org as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: ryan.walker-at-cchmc.org
Name: Ryan Walker

Organization: CCHMC

Title-Subject: [Filtered] Parafin Sections to TEM?

Question: Hello Everyone,

I would like to take sections off a slide and embed them in resin for
TEM and am not entirely sure if this possible.

I am working with nervous tissue (Trigeminal ganglia)which have been
fixed in 2%para 0.5% glut in PBS (perfusion of the mouse) and have
dehydrated them for parafin embeddment and sectioned (approx 12um)
the tissue. In order to localize the area of interest in the ganglia
(this area is very infrequent and quite small) I utilized
immunohistochemistry with the DAB method of detection. The sections
are still attached to slides and I would like (if possible) to use
those sections for the TEM. I am able to get some of the sections
off of the slide albeit this is laborious and very difficult to see
with the naked eye. I do not need perfect ultrastructure therefore I
am not worried about the wear and tear the sections have endured from
the peroxide reaction. However, I would still like to osmicate the
tissue prior to resin embedment. Any suggestions on to process the
sections for TEM would helpful.

Do you think it would be best to just redo the expirement using
agarose embeddment and a vibratome?

Thank you all in advance and for your time!

Ryan

Login Host: 205.142.197.33
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Wed Oct 21 18:50:50 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9LNolVK031483
11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:50:49
-0500
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240808c7054ebeb4da-at-[206.69.208.22]}
11, 11 -- Date: Wed, 21 Oct 2009 18:50:46 -0500
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: ryan.walker-at-cchmc.org (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Parafin Sections to TEM?
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
30, 28 -- From dcromey-at-email.arizona.edu Thu Oct 22 10:21:19 2009
30, 28 -- Received: from mailgator.email.arizona.edu (gremlin.email.arizona.edu [128.196.133.171])
30, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MFLJM0032402
30, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 22 Oct 2009 10:21:19 -0500
30, 28 -- Received: from mailgators_amavis (amavis2.email.arizona.edu [10.0.0.205])
30, 28 -- by mailgator.email.arizona.edu (Postfix) with ESMTP id 76D2F4CDC4B3;
30, 28 -- Thu, 22 Oct 2009 08:21:18 -0700 (MST)
30, 28 -- Received: from RASTER (unknown [150.135.122.85])
30, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id BFED71890732;
30, 28 -- Thu, 22 Oct 2009 08:21:16 -0700 (MST)
30, 28 -- Reply-To: {cromey-at-arizona.edu}
30, 28 -- From: {dcromey-at-email.arizona.edu}
30, 28 -- To: {ryan.walker-at-cchmc.org}
30, 28 -- Cc: "Microscopy listserv" {Microscopy-at-Microscopy.Com}
30, 28 -- References: {200910212351.n9LNpbU7002020-at-ns.microscopy.com}
30, 28 -- In-Reply-To: {200910212351.n9LNpbU7002020-at-ns.microscopy.com}
30, 28 -- Subject: RE: [Microscopy] viaWWW: Parafin Sections to TEM?
30, 28 -- Date: Thu, 22 Oct 2009 08:21:15 -0700
30, 28 -- Organization: University of Arizona
30, 28 -- Message-ID: {000e01ca532b$4dbb7370$e9325a50$-at-arizona.edu}
30, 28 -- MIME-Version: 1.0
30, 28 -- Content-Type: text/plain;
30, 28 -- charset="US-ASCII"
30, 28 -- Content-Transfer-Encoding: 7bit
30, 28 -- X-Mailer: Microsoft Office Outlook 12.0
30, 28 -- Thread-Index: AcpSqW8TBaRl2T0zT3qay7O5ZL69VgAfDB7A
30, 28 -- Content-Language: en-us
30, 28 -- X-Virus-Scanned: amavisd-new at email.arizona.edu
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Thu, 22 Oct 2009 10:48:08 -0500
Subject: [Microscopy] viaWWW: Freon on JEOL JEM 200CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Telmo;
My recollection is that JEOL HT tanks cannot be directly converted
to SF6 because they cannot withstand the required pressure. You will
probably need to purchase a new unit from JEOL.

John Mardinly
Western Digital

-----Original Message-----
X-from: telmonunes-at-hotmail.com [mailto:telmonunes-at-hotmail.com]
Sent: Wednesday, October 21, 2009 5:02 PM
To: John Mardinly

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both telmonunes-at-hotmail.com as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: telmonunes-at-hotmail.com
Name: Telmo

Organization: Faculdade de Ciencias da Universidade de Lisboa

Title-Subject: [Filtered] Freon on JEOL JEM 200CX

Question: I work with a JEOL JEM 200CX and the
freon used as dielectric in the gun has ended, I
would like to exchange this gas to other, because
freon is forbidden to be commercialized (is
responsible for the destruction of the ozone
layer).
My question is, what gas can I use instead the freon?
In high-tension installations its used the
Sulfur hexafluoride (SF6), but I dont know if I
can use it inside the microscope column.

Thank you.

Regards

Telmo

Login Host: 194.117.18.101
---------------------------------------------------------------------------


==============================Original Headers==============================
10, 13 -- From zaluzec-at-microscopy.com Wed Oct 21 18:48:42 2009
10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9LNmeT5025483
10, 13 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:48:42
-0500
10, 13 -- Mime-Version: 1.0
10, 13 -- Message-Id: {p06240805c7054e3e96b6-at-[206.69.208.22]}
10, 13 -- Date: Wed, 21 Oct 2009 18:48:39 -0500
10, 13 -- To: microscopy-at-microscopy.com
10, 13 -- From: telmonunes-at-hotmail.com (by way of MicroscopyListserver)
10, 13 -- Subject: viaWWW: Freon on JEOL JEM 200CX
10, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 13 -- Content-Transfer-Encoding: 8bit
10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9LNmeT5025483
==============================End of - Headers==============================


==============================Original Headers==============================
17, 26 -- From John.Mardinly-at-wdc.com Thu Oct 22 10:48:08 2009
17, 26 -- Received: from wdscexfe01.sc.wdc.com (wdscexfe01.sc.wdc.com [129.253.170.53])
17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MFm7TD015911
17, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 10:48:07 -0500
17, 26 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
17, 26 -- Thu, 22 Oct 2009 08:47:07 -0700
17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
17, 26 -- Content-class: urn:content-classes:message
17, 26 -- MIME-Version: 1.0
17, 26 -- Content-Type: text/plain;
17, 26 -- charset="us-ascii"
17, 26 -- Subject: RE: [Microscopy] viaWWW: Freon on JEOL JEM 200CX
17, 26 -- Date: Thu, 22 Oct 2009 08:47:05 -0700
17, 26 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E11877-at-wdksjexbe01.msj.wdc.com}
17, 26 -- In-Reply-To: {200910220002.n9M026q2009757-at-ns.microscopy.com}
17, 26 -- X-MS-Has-Attach:
17, 26 -- X-MS-TNEF-Correlator:
17, 26 -- Thread-Topic: [Microscopy] viaWWW: Freon on JEOL JEM 200CX
17, 26 -- Thread-Index: AcpSqsGRgsczpKWKS2mR8IrYrpIlwAAg+BmA
17, 26 -- References: {200910220002.n9M026q2009757-at-ns.microscopy.com}
17, 26 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
17, 26 -- To: {telmonunes-at-hotmail.com}
17, 26 -- Cc: {Microscopy-at-microscopy.com}
17, 26 -- X-OriginalArrivalTime: 22 Oct 2009 15:47:07.0044 (UTC) FILETIME=[E8A88640:01CA532E]
17, 26 -- Content-Transfer-Encoding: 8bit
17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9MFm7TD015911
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Thu, 22 Oct 2009 10:54:36 -0500
Subject: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated Iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For particle dispersion a nebulizer should be at the top of the list.

John Mardinly
Western Digital


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Thursday, October 22, 2009 1:05 AM
To: John Mardinly

Hi Jiwen!

Thepreparation of fine particles for TEM and their aggregation is a regular
question on the list. Some search of the archives could really help.
Once, after I gave some advices, the person was kind enough to send my an
answer telling that it worked.

1) Dilute the particles
2) Use volatile solvent like ethanol/methanol/acetone instead of water
(organic solvents may destroy the formvar film, better use carbon grid
without formvar)
3) ultrasonicate just before blotting
4) You may wish to slowly blow on the grid after blotting to speed up drying

It is often the case that particles aggregate due to electrostatic
interactions. In this case, adding NaCl in the solution would decrease the
electrostatic interactions between the particles. Of course the salt will
dry on the grid too. Perhaps you can wash it carefully with water without
washing the particles out, but I have to admit that is risky. Sort of last
chance strategy ;-)

Best regards,
Stephane




----- Original Message ----
X-from: "zhengji-at-mail.nih.gov" {zhengji-at-mail.nih.gov}
To: nizets2-at-yahoo.com
Sent: Thu, October 22, 2009 1:55:12 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both zhengji-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: zhengji-at-mail.nih.gov
Name: Jiwen Zheng

Organization: SAIC-Frederick

Title-Subject: [Filtered] TEM Sample preparation: dextran-coated Iron
oxide nanoparticle

Question: Anyone here has experience on how to prepare dextran-coated
iron oxide nanoparticle (MRI Agent) for TEM?

The protocol I followed is just dropping 3uL of nanoparticles
solution on formvar/carbon coated copper grid and then blotting off
solution after 1 min. The particles on the grid appears highly
aggregated, sometime worm-like and sometime show randomly-aggregated.
Further rinsing with DI water didn't reduce agglomerates. This is
significantly different from the AFM data which suggests most of
particles appear single particle.

Zeta potential measurement show the surface charge of this particle
is close to neutral, so the functionalized grid, such as SMART grid
(silicon grid, Dune science) doesn't help much in such case.

Any suggestions on how to minimize the artifact by optimize the grid
(surface charge, functional group, and hydrophilicity) and particle
deposition.

Thanks in advance!

Login Host: 129.43.32.241
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Wed Oct 21 18:49:31 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9LNnUXL027121
10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009 18:49:31 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240806c7054e649fa7-at-[206.69.208.22]}
10, 12 -- Date: Wed, 21 Oct 2009 18:49:29 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: zhengji-at-mail.nih.gov (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: TEM Sample preparation: dextran-coated Iron oxide
10, 12 -- nanoparticle
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
28, 25 -- From nizets2-at-yahoo.com Thu Oct 22 02:58:22 2009
28, 25 -- Received: from web110810.mail.gq1.yahoo.com
(web110810.mail.gq1.yahoo.com [67.195.13.233])
28, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
n9M7wMR9005573
28, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 02:58:22
-0500
28, 25 -- Received: (qmail 20635 invoked by uid 60001); 22 Oct 2009 07:58:21
-0000
28, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com;
s=s1024; t=1256198301; bh=cmKrxnpatxC3drkg2oeADwJiSEpddo9Yj+/aN80ASr4=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:C
c:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
b=XXvcVqeySg26AiOIFMM41xl4n/SGQkRAvZhxRa0nGclLCJCRHv+Yd/L90KoKE5q/qs5a3bENVN
W7jlvM2oMCU5zEaNolj2YARghYd2XkmiWP/6oAJj7sCpJ5e1O3KeYNQBqjQyu5KFvF8+O2sYXPsP
VPV6U3shcsV/UveO55y5c=
28, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
28, 25 -- s=s1024; d=yahoo.com;
28, 25 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:C
c:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
28, 25 --
b=eVpuyZApd3XcG4ZG7v7PL7U3v7mEsLoht4RiV7yHh1CL+KX8ZnWg3uVChrF99C+SV5tgsKi7ED
qmjyC065E03XZN5R+SiLLuAacBj+VzlynulqqlwF/JwAIskk8J+8V6mVcwMtIPU72O6f5TatTfBZ
aEoV7tnP20xVCBMVLrCqs=;
28, 25 -- Message-ID: {683001.19982.qm-at-web110810.mail.gq1.yahoo.com}
28, 25 -- X-YMail-OSG:
6TLgNv4VM1m4DFjIM42XkFC61WQdhAQP_FQ3eHyOCGCbWx9KixdohZGitAFYvc1ucz.k344DcYro
IkNB5Mc5uQUEs_iHpV6_VnilHQabNV2I9v_vT_DlqCKVRYHUzbxhqsRnl1u11WeJd9B4mytU_3oF
yQ1kBF3vTt55HbGrK4MQeg1xnwjaEe33yca2W.Nf7jxDvoYr5_fW7Ek2CbzHODqa.XDDplLn.OrJ
f2msiRLnOfZCtKESo.YqG1bl2u1QX68TtxBP9i.IsCPTZHsQ6MU9TkKIEyo_FNOQKfUKo.c15hlT
GMtslfmIy30UdCX4FTY7ec63yLyGyhhY.NQeNKCW3.dq_AtVFy8s0KZJukaYAudZn5CblyPmsw--
28, 25 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com
via HTTP; Thu, 22 Oct 2009 00:58:21 PDT
28, 25 -- X-Mailer: YahooMailRC/182.10 YahooMailWebService/0.7.347.3
28, 25 -- References: {200910212355.n9LNtCGZ015665-at-ns.microscopy.com}
28, 25 -- Date: Thu, 22 Oct 2009 00:58:21 -0700 (PDT)
28, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
28, 25 -- Subject: Re: [Microscopy] viaWWW: TEM Sample preparation:
dextran-coated Iron oxide
28, 25 -- To: zhengji-at-mail.nih.gov
28, 25 -- Cc: microscopy-at-microscopy.com
28, 25 -- In-Reply-To: {200910212355.n9LNtCGZ015665-at-ns.microscopy.com}
28, 25 -- MIME-Version: 1.0
28, 25 -- Content-Type: text/plain; charset=iso-8859-1
28, 25 -- Content-Transfer-Encoding: 8bit
28, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id n9M7wMR9005573
==============================End of - Headers==============================


==============================Original Headers==============================
36, 26 -- From John.Mardinly-at-wdc.com Thu Oct 22 10:54:36 2009
36, 26 -- Received: from wdscexfe01.sc.wdc.com (wdscexfe01.sc.wdc.com [129.253.170.53])
36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MFsaeU027546
36, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 10:54:36 -0500
36, 26 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
36, 26 -- Thu, 22 Oct 2009 08:53:35 -0700
36, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
36, 26 -- Content-class: urn:content-classes:message
36, 26 -- MIME-Version: 1.0
36, 26 -- Content-Type: text/plain;
36, 26 -- charset="us-ascii"
36, 26 -- Subject: RE: [Microscopy] Re: viaWWW: TEM Sample preparation: dextran-coated Iron oxide
36, 26 -- Date: Thu, 22 Oct 2009 08:53:29 -0700
36, 26 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E11878-at-wdksjexbe01.msj.wdc.com}
36, 26 -- In-Reply-To: {200910220805.n9M85D06019812-at-ns.microscopy.com}
36, 26 -- X-MS-Has-Attach:
36, 26 -- X-MS-TNEF-Correlator:
36, 26 -- Thread-Topic: [Microscopy] Re: viaWWW: TEM Sample preparation: dextran-coated Iron oxide
36, 26 -- Thread-Index: AcpS7j//9PtLWSF5TAyYaQO02K1ocgAQMy/g
36, 26 -- References: {200910220805.n9M85D06019812-at-ns.microscopy.com}
36, 26 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
36, 26 -- To: {nizets2-at-yahoo.com}
36, 26 -- Cc: {Microscopy-at-microscopy.com}
36, 26 -- X-OriginalArrivalTime: 22 Oct 2009 15:53:35.0512 (UTC) FILETIME=[D0340980:01CA532F]
36, 26 -- Content-Transfer-Encoding: 8bit
36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9MFsaeU027546
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Thu, 22 Oct 2009 12:07:10 -0500
Subject: [Microscopy] RE: viaWWW: Parafin Sections to TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Everyone:

Let me address some of the questions regarding the Quantomix WetSem
Technology.

Short answers:

1) The capsules can only be used in BSE mode because the samples are behind
the imaging membrane, the part that keeps your sample inside and vacuum
outside. In SE mode you are just bouncing electrons off the surface of the
membrane, not entering the specimen chamber (for the most part). Even in BSE
the beam only penetrates a few microns, which leads to question 2.

2) If your specimen, like bacteria in water is not within those few microns
of the membrane or preferably in contact with the membrane you will not see
them. Treating the inside surface with PLL (Poly-L-Lysine) or its opposite
PSS (poly-sodium-4-styrenesulfonate) will allow particles with different
surface charges to "stick" to the imaging membrane where it can be imaged.

3) Since the capsules can only be used in BSE, anything you wish to image
must have a range of elements of differing atomic weights, so electrons in
the probe can be "scattered" producing contrast. This is generally not a
problem for material scientists but poses a huge problem for the biologist,
whom usually have only the basic building blocks of life as we know it
(C,H,O,&N) to work with . This is overcome by using contrast agents like
the ones we're all familiar with in TEM: Os, UA, PTA, PB.

If you adhere to these basic concepts, you will obtain some very nice images
of fully hydrated biological specimens. All of this information is on the
Electron Microscopy Sciences website, where you can download everything you
need to be successful with Quantomix WetSem Technology. If something is
still not clear or working, please feel free to call or email me.


Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

Disclaimer: EMS is the distributor of Quantomic WetSem Technology
--------------------------------------------------
X-from: {nizets2-at-yahoo.com}
Sent: Wednesday, October 21, 2009 10:06 AM
To: {Sampleprep-at-earthlink.net}

HI-
a similar technique for separating the slide from the (re)embedded
section was published by Kai Chien in the 1989 EMSA Proceedings (page
1046). He used a 100 deg. C hotplate. I really like your twist:
supergluing the BEEM capsule on to make a chamber. Much tidier than
dealing with a whole slide in a petri dish!
Lee




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
8, 35 -- From lcgould-at-med.cornell.edu Thu Oct 22 12:07:09 2009
8, 35 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MH79Lm029990
8, 35 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 12:07:09 -0500
8, 35 -- MIME-version: 1.0
8, 35 -- Content-transfer-encoding: 7BIT
8, 35 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed
8, 35 -- Received: from mpx3.med.cornell.edu ([140.251.11.120])
8, 35 -- by mail-gw2.med.cornell.edu
8, 35 -- (Sun Java(tm) System Messaging Server 6.3-8.03 (built Apr 24 2009; 32bit))
8, 35 -- with ESMTP id {0KRX00238E7WZLE0-at-mail-gw2.med.cornell.edu} for
8, 35 -- microscopy-at-microscopy.com; Thu, 22 Oct 2009 13:07:09 -0400 (EDT)
8, 35 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
8, 35 -- by mpx3.med.cornell.edu
8, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.04 64bit (built Jul 2 2009))
8, 35 -- with ESMTPA id {0KRX00ESXE7TOA00-at-mpx3.med.cornell.edu} ; Thu,
8, 35 -- 22 Oct 2009 13:07:08 -0400 (EDT)
8, 35 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379,
8, 35 -- Antispam-Data: 2009.10.22.165116
8, 35 -- X-Perlmx-Spam: Gauge=X, , Probability=10%, Report=' TO_IN_SUBJECT 0.5,
8, 35 -- BODY_SIZE_10000_PLUS 0, OEM_SOFTWARE_X1 0, TO_NO_NAME 0, __C230066_P2 0,
8, 35 -- __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0,
8, 35 -- __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __MEDS_PLAIN 0,
8, 35 -- __MEDS_PLAIN_MEDICATION 0, __MEDS_PLAIN_PHARMACY 0, __MIME_TEXT_ONLY 0,
8, 35 -- __MIME_VERSION 0, __OEM_SOFTWARE_5 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0,
8, 35 -- __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0, __URI_NS '
8, 35 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
8, 35 -- Message-id: {p06230919c70640775f90-at-[140.251.48.23]}
8, 35 -- In-reply-to: {200910221528.n9MFSp8r014181-at-ns.microscopy.com}
8, 35 -- References: {200910221528.n9MFSp8r014181-at-ns.microscopy.com}
8, 35 -- Date: Thu, 22 Oct 2009 13:07:00 -0400
8, 35 -- To: dcromey-at-email.arizona.edu,
8, 35 -- Microscopy Listserver {microscopy-at-microscopy.com}
8, 35 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
8, 35 -- Subject: [Microscopy] RE: viaWWW: Parafin Sections to TEM?
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 22 Oct 2009 12:51:44 -0500
Subject: [Microscopy] Re: viaWWW: Freon on JEOL JEM 200CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Basically, Freon materials are liquids at room temperature
and enter gas phase when hot. This gas is then cooled and
the liquid phase reappears.

SF6 is a gas and you would need pressure to ensure enough
insulation. FEI used to use SF6 in the HV connection at
the top of the column. This SF6 came in a small gas cylinder.

When the tank uses a liquid for insulation, there is no pressure.
Is this the case for your tank?

If you can find out what Freon is in the tank, there are replacements
for some of them:

http://www.hvacmechanic.com/refrigerants/refrigerants.htm

This lists the refrigerants (not used for this in HV tank
applications). Check to see which available material has
the highest dielectric constant. Also see if it not on
the phase out list. Air is also on the list and let's
hope that it is not phased out.

gary g.


At 04:49 PM 10/21/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
15, 22 -- From gary-at-gaugler.com Thu Oct 22 12:51:44 2009
15, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9MHphMk013802
15, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 12:51:44 -0500
15, 22 -- Message-Id: {200910221751.n9MHphMk013802-at-ns.microscopy.com}
15, 22 -- Received: (qmail 27972 invoked from network); 22 Oct 2009 11:27:08 -0700
15, 22 -- Received: by simscan 1.1.0 ppid: 27969, pid: 27970, t: 0.1014s
15, 22 -- scanners: regex: 1.1.0 attach: 1.1.0
15, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
15, 22 -- by smtp1 with SMTP; 22 Oct 2009 11:27:08 -0700
15, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
15, 22 -- Date: Thu, 22 Oct 2009 10:51:32 -0700
15, 22 -- To: telmonunes-at-hotmail.com
15, 22 -- From: Gary Gaugler {gary-at-gaugler.com}
15, 22 -- Subject: Re: [Microscopy] viaWWW: Freon on JEOL JEM 200CX
15, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com}
15, 22 -- In-Reply-To: {200910212349.n9LNnwZv028598-at-ns.microscopy.com}
15, 22 -- References: {200910212349.n9LNnwZv028598-at-ns.microscopy.com}
15, 22 -- Mime-Version: 1.0
15, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
15, 22 -- Content-Transfer-Encoding: 8bit
15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9MHphMk013802
==============================End of - Headers==============================




From: bnross-at-interchange.ubc.ca
Date: Thu, 22 Oct 2009 13:44:17 -0500
Subject: [Microscopy] Re: Balzers MED 010 Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I'd just like to say thanks to everyone that sent in suggestions for fixing the problem. It turned out that one of the two solenoids connected to the vacuum chamber decided to quit working. I bypassed the faulty one and now everything is working and we can carbon coat and glow discharge again!

Thanks again,
-Brad



-----Original Message-----

} Date: Thu Oct 15 15:28:25 PDT 2009
} From: bnross-at-interchange.ubc.ca
} Subject: [Microscopy] Balzers MED 010 Trouble
} To: bnross-at-interchange.ubc.ca
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello everyone,
}
} We have been having a problem with our Balzers MED 010; it won't switch on the high voltage to allow carbon evaporation, glow discharging, etc. The problem was intermittent at first, and could be remedied with a good solid smack on the top of the machine, but now the "HT Free" light and thus the evaporating/sputtering/glow discharging controls remain off thereby rendering the unit quite useless. I checked all the fuses and safety interlock switches with a multimeter and they are all functioning properly. I checked the manual and schematic and discovered there are two "vacuum relays" but no mention of how they determine when the vacuum is sufficiently high to allow the HT to be switched on. I can manually switch on the relays, which turns on the HT Free light, but not the actual HT, as the controls still remain off. I'm guessing there is something wrong with the sensor/gauge that tells the relays that it's ok to switch on the HT. The ultimate vacuum in the chamber seems to b
e!
} fine at around 2x10^-5 - 6x10^-6 mbar as indicated by the main high vacuum gauge. During normal operation in the past, the HT Free light would switch on at around 6x10^-1 mbar.
}
} Has anyone had this problem with an MED 010 before? Any helpful hints, tips, or tricks would be much appreciated!
}
} Thanks,
} --
} Bradford Ross
}
} Microscopy Technician
} BioImaging Facility
} University of British Columbia
} 6270 University Blvd.
} Vancouver, B.C.
} Canada
} V6T 1Z4
}
} phone 604-822-6996
}
}
}
} ==============================Original Headers==============================
} 8, 28 -- From bnross-at-interchange.ubc.ca Thu Oct 15 17:23:35 2009
} 8, 28 -- Received: from mr2.mail-relay.ubc.ca (mr2.mail-relay.ubc.ca [137.82.45.3])
} 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9FMNZbh001448
} 8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 17:23:35 -0500
} 8, 28 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
} 8, 28 -- by mr2.mail-relay.ubc.ca (Postfix) with ESMTP id 5D8221857D
} 8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2009 15:23:34 -0700 (PDT)
} 8, 28 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12])
} 8, 28 -- by smtp.interchange.ubc.ca
} 8, 28 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
} 8, 28 -- with ESMTP id {0KRK00HICU790V-at-smtp.interchange.ubc.ca} for
} 8, 28 -- Microscopy-at-microscopy.com; Thu, 15 Oct 2009 15:23:34 -0700 (PDT)
} 8, 28 -- Date: Thu, 15 Oct 2009 15:23:33 -0700 (PDT)
} 8, 28 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
} 8, 28 -- Subject: Balzers MED 010 Trouble
} 8, 28 -- To: Microscopy-at-microscopy.com
} 8, 28 -- Message-id: {33514548.36931255645413245.JavaMail.myubc2-at-brahms.my.ubc.ca}
} 8, 28 -- MIME-version: 1.0
} 8, 28 -- X-Mailer: uPortal WEB email client 3.0
} 8, 28 -- Content-type: text/plain; charset=us-ascii
} 8, 28 -- X-UBC-Scanned: Sophos PureMessage 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.10.15.220928
} 8, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
} 8, 28 -- X-PerlMx-Spam: Probability=8%, Report=
} 8, 28 -- SUPERLONG_LINE 0.05, BODY_SIZE_1500_1599 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0
} 8, 28 -- X-Spam-Level:
} 8, 28 -- X-Spam-Flag: No
} 8, 28 -- Content-Transfer-Encoding: 8bit
} 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9FMNZbh001448
} ==============================End of - Headers==============================
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-6996


==============================Original Headers==============================
10, 27 -- From bnross-at-interchange.ubc.ca Thu Oct 22 13:44:16 2009
10, 27 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MIiGAr031238
10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 13:44:16 -0500
10, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70])
10, 27 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP id 74AB015B5E
10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 11:44:15 -0700 (PDT)
10, 27 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
10, 27 -- by smtp.interchange.ubc.ca
10, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
10, 27 -- with ESMTP id {0KRX00I8EIPQLC-at-smtp.interchange.ubc.ca} for
10, 27 -- Microscopy-at-microscopy.com; Thu, 22 Oct 2009 11:44:15 -0700 (PDT)
10, 27 -- Date: Thu, 22 Oct 2009 11:44:14 -0700 (PDT)
10, 27 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
10, 27 -- Subject: Re: [Microscopy] Balzers MED 010 Trouble
10, 27 -- To: Microscopy-at-microscopy.com
10, 27 -- Message-id: {16759303.28551256237054289.JavaMail.myubc2-at-handel.my.ubc.ca}
10, 27 -- MIME-version: 1.0
10, 27 -- X-Mailer: uPortal WEB email client 3.0
10, 27 -- Content-type: text/plain; charset=us-ascii
10, 27 -- Content-transfer-encoding: 7bit
10, 27 -- X-UBC-Scanned: Sophos PureMessage 5.5.6.374947, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.22.183616
10, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
10, 27 -- X-PerlMx-Spam: Probability=8%, Report=
10, 27 -- SUPERLONG_LINE 0.05, BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __LINES_OF_YELLING 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __URI_NS
10, 27 -- X-Spam-Level:
10, 27 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: jteshima-at-dunesciences.com
Date: Thu, 22 Oct 2009 15:21:59 -0500
Subject: [Microscopy] Fwd: RE: viaWWW: TEM Sample preparation: dextran-coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jiwen,
We have used SMART Grids to prepare nanoparticle samples that are
neutral in charge. They are both hydrophyllic. Have a look at the
PEG-terminated and hydroxyl-terminated grid descriptions on our
website. We have also posted a nice video showing one method of
preparing nanoparticle samples for TEM or other characterization
methods.

http://www.dunesciences.com/peg.html
http://www.dunesciences.com/hydroxyl.html
http://www.dunesciences.com/sample_prep_video.html

Janet

Janet Teshima
SMART Grids Product Manager
cell: 503-5447526
office: 541-636-3712
jteshima-at-dunesciences.com
www.dunesciences.com

Begin forwarded message:

} From: eschumacher-at-mccrone.com
} Date: October 22, 2009 6:01:36 AM PDT
} To: jteshima-at-dunesciences.com
} Subject: [Microscopy] RE: viaWWW: TEM Sample preparation: dextran-
} coated
} Reply-To: eschumacher-at-mccrone.com
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Jiwen,
}
} I've had the best success with these materials using a combination
} of some of the suggestions already posted. You're correct, the
} samples consist of single nanoparticles, but aggregation during
} drying on the grid is an issue. I use copper grids coated with
} carbon film, no formvar. The samples I've worked with have been
} either aqueous suspensions or dried solids. I use distilled water
} to either dilute the liquid or re-suspend the solid, and I dilute
} until I have a suspension with a faint orange color. I put one drop
} of this onto a grid and wick away most of the excess liquid,
} allowing the remaining thin film to air dry. If the liquid is still
} somewhat viscous after dilution and coalesces on the grid while
} drying, it can help to gently drag the drop across the grid film a
} couple of times, using a the tip of a fine pair of forceps or an
} eyelash brush. This will help to deposit the particles across the
} film.
}
} While some samples will disperse nicely as single particles or small
} aggregates, most times I find a mix of areas with dispersed and
} aggregated particles. The dispersed particles will be best for
} imaging, but you'll want to examine the larger aggregates also, to
} ensure that you've sampled representatively, and that there hasn't
} been size segregation as the film has dried across the grid.
}
} Hope this helps.
}
} Regards,
}
} Elaine
}
}
} *********************************************************************
} Elaine F.Schumacher
} Senior Research Scientist
} McCrone Associates, Inc.
} 850 Pasquinelli Drive
} Westmont, IL 60559-5539 USA
} 630-887-7100 (tel)
} 630-887-7417 (fax)
} E-mail: eschumacher-at-mccrone.com
} Web Site: www.mccrone.com
}
}
}
} *********************************************************************
} This message and any attachments are solely for the
} intended recipient. If you are not the intended recipient,
} disclosure, copying, use or distribution of the information
} included in this message is prohibited.
} *********************************************************************
}
} -----Original Message-----
} X-from: zhengji-at-mail.nih.gov [mailto:zhengji-at-mail.nih.gov]
} Sent: Wednesday, October 21, 2009 7:03 PM
} To: Elaine F. Schumacher
} Subject: [Microscopy] viaWWW: TEM Sample preparation: dextran-coated
} Iron oxide
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both zhengji-at-mail.nih.gov as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: zhengji-at-mail.nih.gov
} Name: Jiwen Zheng
}
} Organization: SAIC-Frederick
}
} Title-Subject: [Filtered] TEM Sample preparation: dextran-coated
} Iron oxide nanoparticle
}
} Question: Anyone here has experience on how to prepare dextran-
} coated iron oxide nanoparticle (MRI Agent) for TEM?
}
} The protocol I followed is just dropping 3uL of nanoparticles
} solution on formvar/carbon coated copper grid and then blotting off
} solution after 1 min. The particles on the grid appears highly
} aggregated, sometime worm-like and sometime show randomly-aggregated.
} Further rinsing with DI water didn't reduce agglomerates. This is
} significantly different from the AFM data which suggests most of
} particles appear single particle.
}
} Zeta potential measurement show the surface charge of this particle
} is close to neutral, so the functionalized grid, such as SMART grid
} (silicon grid, Dune science) doesn't help much in such case.
}
} Any suggestions on how to minimize the artifact by optimize the grid
} (surface charge, functional group, and hydrophilicity) and particle
} deposition.
}
} Thanks in advance!
}
} Login Host: 129.43.32.241
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 10, 12 -- From zaluzec-at-microscopy.com Wed Oct 21 18:49:31 2009 10,
} 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n9LNnUXL027121
} 10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 21 Oct 2009
} 18:49:31 -0500
} 10, 12 -- Mime-Version: 1.0
} 10, 12 -- Message-Id: {p06240806c7054e649fa7-at-[206.69.208.22]}
} 10, 12 -- Date: Wed, 21 Oct 2009 18:49:29 -0500 10, 12 -- To: microscopy-at-microscopy.com
} 10, 12 -- From: zhengji-at-mail.nih.gov (by way of
} MicroscopyListserver) 10, 12 -- Subject: viaWWW: TEM Sample
} preparation: dextran-coated Iron oxide 10, 12 -- nanoparticle 10,
} 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 28, 29 -- From eschumacher-at-mccrone.com Thu Oct 22 07:54:35 2009
} 28, 29 -- Received: from oma.mccrone.com (mail.mccrone.com
} [12.54.22.114])
} 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id n9MCsYUI031847
} 28, 29 -- for {microscopy-at-microscopy.com} ; Thu, 22 Oct 2009
} 07:54:34 -0500
} 28, 29 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by
} 28, 29 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Thu, 22
} Oct 2009 07:54:29
} 28, 29 -- -0500
} 28, 29 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
} 28, 29 -- To: "zhengji-at-mail.nih.gov" {zhengji-at-mail.nih.gov} ,
} 28, 29 -- "microscopy-at-microscopy.com"
} 28, 29 -- {microscopy-at-microscopy.com}
} 28, 29 -- Date: Thu, 22 Oct 2009 07:54:30 -0500
} 28, 29 -- Subject: RE: [Microscopy] viaWWW: TEM Sample preparation:
} dextran-coated
} 28, 29 -- Iron oxide
} 28, 29 -- Thread-Topic: [Microscopy] viaWWW: TEM Sample preparation:
} dextran-coated
} 28, 29 -- Iron oxide
} 28, 29 -- Thread-Index: AcpSqvt1hF5B0vfcRGCTs+MADfEGCQAakFaw
} 28, 29 -- Message-ID: {874B1DB532886444A60A015EE363493F0B760E41CC-at-mccronemsg07.tmg.mccrone.com
} }
} 28, 29 -- References: {200910220002.n9M02gfn012223-at-ns.microscopy.com}
} 28, 29 -- In-Reply-To: {200910220002.n9M02gfn012223-at-ns.microscopy.com}
} 28, 29 -- Accept-Language: en-US
} 28, 29 -- Content-Language: en-US
} 28, 29 -- X-MS-Has-Attach:
} 28, 29 -- X-MS-TNEF-Correlator:
} 28, 29 -- acceptlanguage: en-US
} 28, 29 -- Content-Type: text/plain; charset="us-ascii"
} 28, 29 -- MIME-Version: 1.0
} 28, 29 -- Content-Transfer-Encoding: 8bit
} 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id n9MCsYUI031847
} ==============================End of -
} Headers==============================





==============================Original Headers==============================
10, 22 -- From jteshima-at-dunesciences.com Thu Oct 22 15:21:59 2009
10, 22 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MKLwN1018334
10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 15:21:58 -0500
10, 22 -- Received: by pzk37 with SMTP id 37so6303001pzk.10
10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Oct 2009 13:21:58 -0700 (PDT)
10, 22 -- Received: by 10.114.45.2 with SMTP id s2mr14935476was.122.1256242917146;
10, 22 -- Thu, 22 Oct 2009 13:21:57 -0700 (PDT)
10, 22 -- Received: from ?192.168.1.101? (c-67-160-157-47.hsd1.or.comcast.net [67.160.157.47])
10, 22 -- by mx.google.com with ESMTPS id 21sm2131160pzk.11.2009.10.22.13.21.55
10, 22 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
10, 22 -- Thu, 22 Oct 2009 13:21:56 -0700 (PDT)
10, 22 -- Message-Id: {35A0E3A9-F605-4A00-963D-9B293E6DD6E3-at-dunesciences.com}
10, 22 -- From: Janet Teshima {jteshima-at-dunesciences.com}
10, 22 -- To: Microscopy-at-microscopy.com
10, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 22 -- Content-Transfer-Encoding: 7bit
10, 22 -- Mime-Version: 1.0 (Apple Message framework v936)
10, 22 -- Subject: Fwd: [Microscopy] RE: viaWWW: TEM Sample preparation: dextran-coated
10, 22 -- Date: Thu, 22 Oct 2009 13:21:54 -0700
10, 22 -- References: {200910221301.n9MD1aKQ013166-at-ns.microscopy.com}
10, 22 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: ron.doole-at-materials.ox.ac.uk
Date: Fri, 23 Oct 2009 04:36:56 -0500
Subject: [Microscopy] TEM Probe size in CBED mode

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Vishnu,

A JEOL 2010 could be in several different configurations so you may not be able to follow this exactly. I am assuming that you have a standard URP or ARP pole-piece, the 'Mode selector' with EDS, NBD and CBD options and a CCD image capture.

You can measure the probe size by setting up your CBD conditions, switching from DIFF to MAG and then moving the sample out of the beam so that you can record the probe, you will need to be at the highest magnification and you will need to ensure that the image is focussed and stigmated at this magnification. On the JEOL 2010 it is relatively easy to to this as you can set the high mag image up in TEM and switch to the CDB mode. If you align the beam between the two modes it returns pretty accurately after two or three iterations to remove the hysteresis. You then need to take an intensity profile across the probe, the size is usually defined as FWHM when the probe does not saturate the CCD. Make sure you start with a very short exposure time, the shortest possible, so that you do not damage the CCD, and if possible increase it to get a reasonable count rate. If you are using a gun shutter (beam deflector) you may see some streaking as the beam is deflected on and off the CCD. You will need to judge if the stationary probe shape on the CCD has been affected by the deflection on and off the CCD.

If you use film the procedure is similar but you don't know if you have the exposure right until you've developed them so take a good range of exposures.

However, it is not only the probe size that you need to know it is also the probe stability, if your CBD exposure is going to be 5 sec does your probe move over that time? If so it effectively increases your probe size as it increases the area analysed during the exposure. If you take several exposures they should all give the same answer for probe size, if the probe is moving the longer exposures will be larger (probe jittering around) or misshapen (probe drifting). With a CCD camera it is possible to see if the probe is stationary, if you only have film you may have to rely on the focus screen and binoculars.
Depending on your instrument you may find that the probe drifts for a while after selecting CBD mode, this is probably due to the thermal stabilty of the condenser lenses as they change from one power setting to another as there is quite a large change in lens currents. For the smallest probes on my 2010 I had to wait for between 20 and 40 minutes for the probe to be completely stationary in CBD mode. Then I could switch to TEM for a short while (long enough to check my specimen image and focus) and back to CBD with very little probe drift.

The specimen stability is also important.

Good luck,
Ron


Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: vishnu.mogili-at-gmail.com [vishnu.mogili-at-gmail.com]
Sent: 22 October 2009 16:17
To: Ron Doole

Dear listers,

Can anyone tell me how we can measure probe size of
Jeol 2010 TEM having LaB6 filament working under CBED mode?

I want to measure strain between 10nm thick Silicon-Germanium and
Silicon layers. So I think I should use smallest available probe to
acheive it.


Thanks,

Vishnu Mogili

Materials & Surface Science Institute
University of Limerick
Ireland

==============================Original Headers==============================
7, 29 -- From vishnu.mogili-at-gmail.com Thu Oct 22 10:10:43 2009
7, 29 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.156])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9MFAg9W017674
7, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 22 Oct 2009 10:10:43 -0500
7, 29 -- Received: by fg-out-1718.google.com with SMTP id e21so3422992fga.0
7, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 22 Oct 2009 08:10:42 -0700 (PDT)
7, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 29 -- d=gmail.com; s=gamma;
7, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
7, 29 -- :from:to:content-type;
7, 29 -- bh=iHDK3+ORK3b5k4eWunJwIpSFqSVDVHMDOjViT6J8TtI=;
7, 29 -- b=GyqcfilfcVWCdMrSrhWbHXbDAXgi5B1sJFcWfoClrXf5mz4ct6BYkbazHxp2xXq1EW
7, 29 -- 3Dws7BMa8tTiqZt4FK8TyZVkOzHoEFpzP1vMv3KK+INCqFXk7gKKhv4FkKB4DbRn2MoY
7, 29 -- 40Z/wl95N6e1uGxnedo3Xj40gldpP0lkcHv3Y=
7, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
7, 29 -- d=gmail.com; s=gamma;
7, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
7, 29 -- b=a18jZ8HviIi3Nc2X8N78hxjcL13f/PB/AGNPy5bFLdaD5DfvQTwLWmao69zWacYNTp
7, 29 -- z56dp331cUDeYV6a+F7qGFrhdRBCpjHo7qhpfFjLXQZdLUwWI4JdjMPsqUOyRHtYflhb
7, 29 -- 3ywzM+UWAiRWpmfgjQqUJ1PS+q5hcLk7ootUg=
7, 29 -- MIME-Version: 1.0
7, 29 -- Received: by 10.86.240.9 with SMTP id n9mr5066645fgh.70.1256224241956; Thu, 22
7, 29 -- Oct 2009 08:10:41 -0700 (PDT)
7, 29 -- Date: Thu, 22 Oct 2009 16:10:41 +0100
7, 29 -- Message-ID: {95c1e3310910220810g7d407f52o8971d50064cf063b-at-mail.gmail.com}
7, 29 -- Subject: TEM Probe size in CBED mode
7, 29 -- From: vishnu mogili {vishnu.mogili-at-gmail.com}
7, 29 -- To: Microscopy-at-Microscopy.Com
7, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================

==============================Original Headers==============================
18, 30 -- From ron.doole-at-materials.ox.ac.uk Fri Oct 23 04:36:56 2009
18, 30 -- Received: from relay2.mail.ox.ac.uk (relay2.mail.ox.ac.uk [163.1.2.161])
18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9N9at6r029835
18, 30 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2009 04:36:55 -0500
18, 30 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk)
18, 30 -- by relay2.mail.ox.ac.uk with esmtp (Exim 4.69)
18, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
18, 30 -- id 1N1GZq-0004cu-8l; Fri, 23 Oct 2009 10:36:54 +0100
18, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.2.156]) by
18, 30 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Fri, 23 Oct 2009 10:36:53
18, 30 -- +0100
18, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
18, 30 -- To: "vishnu.mogili-at-gmail.com" {vishnu.mogili-at-gmail.com}
18, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
18, 30 -- Date: Fri, 23 Oct 2009 10:36:54 +0100
18, 30 -- Subject: RE: [Microscopy] TEM Probe size in CBED mode
18, 30 -- Thread-Topic: [Microscopy] TEM Probe size in CBED mode
18, 30 -- Thread-Index: AcpTKr16h7J+YhUNT0mpfk8sqZnSrwABCizj
18, 30 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF9264C21C479-at-EXMBX02.ad.oak.ox.ac.uk}
18, 30 -- References: {200910221517.n9MFHDle032120-at-ns.microscopy.com}
18, 30 -- In-Reply-To: {200910221517.n9MFHDle032120-at-ns.microscopy.com}
18, 30 -- Accept-Language: en-US, en-GB
18, 30 -- Content-Language: en-GB
18, 30 -- X-MS-Has-Attach:
18, 30 -- X-MS-TNEF-Correlator:
18, 30 -- acceptlanguage: en-US, en-GB
18, 30 -- Content-Type: text/plain; charset="us-ascii"
18, 30 -- MIME-Version: 1.0
18, 30 -- Content-Transfer-Encoding: 8bit
18, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9N9at6r029835
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Fri, 23 Oct 2009 17:19:11 -0500
Subject: [Microscopy] Monyl filtration cloth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Can anyone advise a supplier of the high-quality synthetic cloth used for filtration, brand
name Monyl?

I have a few old pieces, it seems that it was made by Züricher Beuteltuchfabrik AG (ZBF),
CH-8803 Rüschlikon, Switzerland, and that this company has been absorbed by or has
evolved to become Sefar (www.sefar.com) but I'm unable to locate the product on their
website.

cheers
Ritchie

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



==============================Original Headers==============================
7, 29 -- From r.sims-at-auckland.ac.nz Fri Oct 23 17:19:10 2009
7, 29 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9NMJ76m011517
7, 29 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2009 17:19:10 -0500
7, 29 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 29 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id F16D59ED72
7, 29 -- for {microscopy-at-microscopy.com} ; Sat, 24 Oct 2009 11:19:06 +1300 (NZDT)
7, 29 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz
7, 29 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1])
7, 29 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024)
7, 29 -- with ESMTP id E7AGVKf4xgit for {microscopy-at-microscopy.com} ;
7, 29 -- Sat, 24 Oct 2009 11:19:06 +1300 (NZDT)
7, 29 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18])
7, 29 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits))
7, 29 -- (No client certificate requested)
7, 29 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id D07299ED6B
7, 29 -- for {microscopy-at-microscopy.com} ; Sat, 24 Oct 2009 11:19:06 +1300 (NZDT)
7, 29 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
7, 29 -- To: microscopy-at-microscopy.com
7, 29 -- Date: Sat, 24 Oct 2009 11:22:50 +1300
7, 29 -- MIME-Version: 1.0
7, 29 -- Subject: Monyl filtration cloth
7, 29 -- Message-ID: {4AE2E38A.12182.106F46-at-r.sims.auckland.ac.nz}
7, 29 -- Priority: normal
7, 29 -- X-mailer: Pegasus Mail for Windows (4.41)
7, 29 -- Content-type: text/plain; charset=ISO-8859-1
7, 29 -- Content-description: Mail message body
7, 29 -- Content-Transfer-Encoding: 8bit
7, 29 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n9NMJ76m011517
==============================End of - Headers==============================




From: jbs-at-temple.edu
Date: Sun, 25 Oct 2009 14:36:18 -0500
Subject: [Microscopy] viaWWW: Mini SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jbs-at-temple.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jbs-at-temple.edu
Name: Joel Sheffield

Organization: Temple University

Title-Subject: [Filtered] Mini SEM

Question: I posted this directly to the listserv, and it didn't seem
to go. If it should be a double posting, my apologies.

Hi Colleagues,

We are interested in getting one of the new bench-top SEM instruments
to complement our small microscopy facility. I am aware of
instruments by Nikon (JEOL), FEI (Phenom), Evex, and Novelx (Field
Emission Gun). If any of you have experience or thoughts about any
of these instruments, I would appreciate your comments.

Thank you very much.

Joel


--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: jbs-at-temple.edu
URL: http://astro.temple.edu/~jbs


Login Host: 69.136.69.159
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Sun Oct 25 14:36:15 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9PJaElJ015229
15, 11 -- for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 14:36:14 -0500
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240807c70a58ea44de-at-[206.69.208.22]}
15, 11 -- Date: Sun, 25 Oct 2009 14:36:12 -0500
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: jbs-at-temple.edu (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Mini SEM
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kraftpiano-at-gmail.com
Date: Sun, 25 Oct 2009 15:35:28 -0500
Subject: [Microscopy] A call for aid with an SEM in Santa Barbara, CA.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings!

I have secured a JSM-35C donation from a company in Santa Barbara, CA
for a new educational outreach program in Miami, FL.
This will be the first major piece of equipment in a lab to be
supported by local high- and middle-schools to provide high-end
science for students who might not otherwise have any access to real
labs.

It is sitting in a warehouse, ready to be shipped, but we need to get
it de-cabled first. The two halves are still joined, and it is
currently on 4x4s in the warehouse.

I was wondering if anybody on the list is in the Santa Barbara area
and would mind volunteering to go over and un-hook the cables and give
some guidance to preparing it for shipping cross country.

If anyone is willing to help out, please contact me off list.

Thanks,

Justin A. Kraft
Center for Inquiry Based Science Education

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
8, 29 -- From kraftpiano-at-gmail.com Sun Oct 25 15:35:28 2009
8, 29 -- Received: from mail-fx0-f228.google.com (mail-fx0-f228.google.com [209.85.220.228])
8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9PKZR1Z031341
8, 29 -- for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 15:35:28 -0500
8, 29 -- Received: by fxm28 with SMTP id 28so12045447fxm.18
8, 29 -- for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 13:35:27 -0700 (PDT)
8, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 29 -- d=gmail.com; s=gamma;
8, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
8, 29 -- :from:to:content-type;
8, 29 -- bh=50FqOfiRFk8sgrVRq12dPjp+X0qnkEr9nPemGZR+dLc=;
8, 29 -- b=pZKGHL2jMPVigTvFqctWFCI5Qh20V1yrsOSyTPhVwjALXMB82SLl24/HKCQTG6bQeO
8, 29 -- IA1RVsZc4Pu3fMiuXhf77DfImDHDB7W6EoAehhfPano1tF7Dg57ZbgyGb1x2CFuQdhVW
8, 29 -- 72cIwpyVoaG9v2dBBl4hjmmy4PRjra6KOK0NI=
8, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
8, 29 -- d=gmail.com; s=gamma;
8, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
8, 29 -- b=sv/faZHHSBfkLJ8tPtrOVVMVDDEJHVihfvJD8ew+uhrfPvyodrQOEawz41HVVUM2Rr
8, 29 -- 38kXZ17cXQKAMGYgeY1jseQN0cE5dYTz90u0XZXCaFGfLh94nnOI6Feh0i8NKBpHDfQ0
8, 29 -- xI54thqlkcvHG/XhzINqnzop4U8yyQPNtcqNw=
8, 29 -- MIME-Version: 1.0
8, 29 -- Received: by 10.204.154.150 with SMTP id o22mr8383491bkw.154.1256502927004;
8, 29 -- Sun, 25 Oct 2009 13:35:27 -0700 (PDT)
8, 29 -- Date: Sun, 25 Oct 2009 13:35:26 -0700
8, 29 -- Message-ID: {25e2b0d20910251335m7afbb56ch4761b91101ed3e3-at-mail.gmail.com}
8, 29 -- Subject: A call for aid with an SEM in Santa Barbara, CA.
8, 29 -- From: Justin Kraft {kraftpiano-at-gmail.com}
8, 29 -- To: microscopy-at-microscopy.com
8, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: leech-at-sfsu.edu
Date: Sun, 25 Oct 2009 16:29:28 -0500
Subject: [Microscopy] viaWWW: Electron Microscopy Facility Manager position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both leech-at-sfsu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: leech-at-sfsu.edu
Name: Mary Leech

Organization: San Francisco State University

Title-Subject: [Filtered] Electron Microscopy
Facility Manager position available

Question: Electron Microscopy Facility Manager
position available at San Francisco State
University

Position Summary: Responsible for providing
support to teaching, research, and outreach
efforts of the College of Science, Departments of
Chemistry & Biochemistry, Biology, Geosciences,
Physics, and School of Engineering; overseeing
the Electron Microscopy Facility; instructs
students, staff, and faculty in the use of the
Scanning Electron Microscope (SEM), Transmission
Electron Microscope (TEM) and sample preparation;
develops and maintains protocols for the
efficient and safe use of the equipment and
chemicals related to the electron microscopy.

Description: Part-time (.50) with the possibility
of becoming Full-time. Anticipated Hiring Range:
$2,209-$2,761 per month

Minimum qualifications: BS or BA in physics,
chemistry, earth science, biology, or related
field. Work Experience: three years experience in
technical research; 2-3 years experience in the
maintenance and operation of Scanning Electron
Microscope (SEM) and Transmission Electron
Microscope (TEM) equipment; statistical work
experience.

Preferred qualifications: MS or PhD in a physical
science field; work experience in facility
management; experience working with SEM and TEM
on a wide variety of sample types; experience
with Energy Dispersive Spectroscopy (EDS) and
Electron Back-Scatter Diffraction (EBSD)
accessories; basic knowledge of electronics;
knowledge/use of computers; trouble-shooting and
repair skills.

Position is open until filled and applications are currently being reviewed

Full position description available at:
https://cmsweb.sfsu.edu/psp/HSFPRDF/CUSTOMER/HRMS/c/HRS_HRAM.HRS_CE.GBL,
Research Technician III ñ College of Science &
Engineering, Job ID: 1977

How To Apply:
Submit an application and/or resume and cover
letter (optional), describing your specific
qualifications for this position.

All applicants must submit a SF State
Staff/Administrator Application and/or resume
with an original signature for each job posting.
All application material(s) must include the job
posting number (Job ID: 1977). To be considered,
application material must be submitted in person
or through U.S. mail to Human Resources; San
Francisco State University does not accept
on-line, e-mail, or faxed application materials
at this time.

A SF State Staff/Administrator Application is required for each job posting:

For a Word copy of the Staff/Administrator
application, cut and paste the following internet
address into your web browser:
http://www.sfsu.edu/~hrwww/online_forms/employment/sfsu_staff_admin_app.doc

For a PDF version of the Staff/Administrator
application, copy and paste the following
internet address into your web browser:
http://www.sfsu.edu/~hrwww/online_forms/employment/sfsu_staff_admin_app.pdf

Mail application material(s) to:

San Francisco State University
Human Resources, Safety & Risk Management - Staff Employment Services
1600 Holloway Avenue, Administration Building 252,
San Francisco, CA 94132-4252

The Human Resources, Safety & Risk Management
office is open Mondays, Tuesdays, Thursdays and
Fridays from 8 a.m. to 5 p.m., and can be reached
at (415) 338-1872 or (415) 338-1873; TDD (415)
338-3040. Our office is in-service and closed to
the public on Wednesdays.

SF State is an Equal Opportunity/Americans with
Disabilities Act employer and has a strong
commitment to the principles of diversity.


Login Host: 69.19.14.23
---------------------------------------------------------------------------


==============================Original Headers==============================
23, 13 -- From zaluzec-at-microscopy.com Sun Oct 25 16:29:28 2009
23, 13 -- Received: from [206.69.208.22] ([130.202.238.72])
23, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9PLTQkG015112
23, 13 -- for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 16:29:27 -0500
23, 13 -- Mime-Version: 1.0
23, 13 -- Message-Id: {p06240808c70a738c82f6-at-[206.69.208.22]}
23, 13 -- Date: Sun, 25 Oct 2009 16:29:25 -0500
23, 13 -- To: microscopy-at-microscopy.com
23, 13 -- From: leech-at-sfsu.edu (by way of MicroscopyListserver)
23, 13 -- Subject: viaWWW: Electron Microscopy Facility Manager position available
23, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
23, 13 -- Content-Transfer-Encoding: 8bit
23, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9PLTQkG015112
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Sun, 25 Oct 2009 22:12:24 -0500
Subject: [Microscopy] Re: viaWWW: Electron Microscopy Facility Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Absurd. Leech is a good moniker ID.

And it costs about $5k/month for a flop house.

gary g.


At 02:30 PM 10/25/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
10, 23 -- From gary-at-gaugler.com Sun Oct 25 22:12:24 2009
10, 23 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9Q3COJm012955
10, 23 -- for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 22:12:24 -0500
10, 23 -- Message-Id: {200910260312.n9Q3COJm012955-at-ns.microscopy.com}
10, 23 -- Received: (qmail 15737 invoked from network); 25 Oct 2009 19:53:11 -0700
10, 23 -- Received: by simscan 1.1.0 ppid: 15734, pid: 15735, t: 0.1148s
10, 23 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 23 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
10, 23 -- by smtp2 with SMTP; 25 Oct 2009 19:53:11 -0700
10, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
10, 23 -- Date: Sun, 25 Oct 2009 20:12:21 -0700
10, 23 -- To: leech-at-sfsu.edu
10, 23 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 23 -- Subject: Re: [Microscopy] viaWWW: Electron Microscopy Facility Manager
10, 23 -- position available
10, 23 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 23 -- In-Reply-To: {200910252130.n9PLUeaj017399-at-ns.microscopy.com}
10, 23 -- References: {200910252130.n9PLUeaj017399-at-ns.microscopy.com}
10, 23 -- Mime-Version: 1.0
10, 23 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9Q3COJm012955
==============================End of - Headers==============================




From: jared-at-lv-em.com
Date: Mon, 26 Oct 2009 11:00:10 -0500
Subject: [Microscopy] Re: viaWWW: Mini SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Joel,

I recommend you also consider the LVEM5 benchtop electron microscope.
Not only is this a benchtop SEM with 2nm resolution, but it is also
acts as a TEM and STEM.

Please contact me off the list-serve for more details.

Regards,

----------------------------

Jared Lapkovsky
Sales and Applications Engineer

LVEM5 Team
Delong America Inc.

Montreal, Canada

514.904.1202 ext. 701
www.lv-em.com


Come meet us at:

MRS Fall Exhibit (Hynes Convention Center, Boston, MA) December 1 - 3, Booth 909


On Sun, Oct 25, 2009 at 3:48 PM, {jbs-at-temple.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at  http://microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please  copy  both jbs-at-temple.edu as well as   the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: jbs-at-temple.edu
} Name: Joel Sheffield
}
} Organization: Temple University
}
} Title-Subject: [Filtered] Mini SEM
}
} Question: I posted this directly to the listserv, and it didn't seem
} to go.  If it should be a double posting, my apologies.
}
} Hi Colleagues,
}
} We are interested in getting one of the new bench-top SEM instruments
} to complement our small microscopy facility.  I am aware of
} instruments by Nikon (JEOL), FEI (Phenom), Evex, and Novelx (Field
} Emission Gun).  If any of you have experience or thoughts about any
} of these instruments, I would appreciate your comments.
}
} Thank you very much.
}
} Joel
}
}
} --
}
}
} Joel B. Sheffield, Ph.D
} Department of Biology
} Temple University
} Philadelphia, PA 19122
} Voice: 215 204 8839
} e-mail: jbs-at-temple.edu
} URL:  http://astro.temple.edu/~jbs
}
}
}  Login Host: 69.136.69.159
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 15, 11 -- From zaluzec-at-microscopy.com Sun Oct 25 14:36:15 2009
} 15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 15, 11 --       by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9PJaElJ015229
} 15, 11 --       for {microscopy-at-microscopy.com} ; Sun, 25 Oct 2009 14:36:14 -0500
} 15, 11 -- Mime-Version: 1.0
} 15, 11 -- Message-Id: {p06240807c70a58ea44de-at-[206.69.208.22]}
} 15, 11 -- Date: Sun, 25 Oct 2009 14:36:12 -0500
} 15, 11 -- To: microscopy-at-microscopy.com
} 15, 11 -- From: jbs-at-temple.edu (by way of MicroscopyListserver)
} 15, 11 -- Subject: viaWWW: Mini SEM
} 15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


==============================Original Headers==============================
15, 33 -- From jared-at-lv-em.com Mon Oct 26 11:00:10 2009
15, 33 -- Received: from washington.serverhostcenter.com (washington.serverhostcenter.com [80.251.16.2])
15, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9QG09d9001998
15, 33 -- for {microscopy-at-microscopy.com} ; Mon, 26 Oct 2009 11:00:09 -0500
15, 33 -- Received: from mail-yx0-f189.google.com ([209.85.210.189])
15, 33 -- by washington.serverhostcenter.com with esmtpsa (TLSv1:RC4-MD5:128)
15, 33 -- (Exim 4.69)
15, 33 -- (envelope-from {jared-at-lv-em.com} )
15, 33 -- id 1N2RzL-0006Wg-Ab
15, 33 -- for microscopy-at-microscopy.com; Mon, 26 Oct 2009 11:00:07 -0500
15, 33 -- Received: by yxe27 with SMTP id 27so10607820yxe.10
15, 33 -- for {microscopy-at-microscopy.com} ; Mon, 26 Oct 2009 09:00:06 -0700 (PDT)
15, 33 -- MIME-Version: 1.0
15, 33 -- Received: by 10.101.27.20 with SMTP id e20mr9583082anj.137.1256572806257; Mon,
15, 33 -- 26 Oct 2009 09:00:06 -0700 (PDT)
15, 33 -- In-Reply-To: {200910251948.n9PJmTdm029116-at-ns.microscopy.com}
15, 33 -- References: {200910251948.n9PJmTdm029116-at-ns.microscopy.com}
15, 33 -- Date: Mon, 26 Oct 2009 12:00:06 -0400
15, 33 -- Message-ID: {3629bb860910260900y1979af94m43cd76b13d06a505-at-mail.gmail.com}
15, 33 -- Subject: Re: [Microscopy] viaWWW: Mini SEM
15, 33 -- From: Jared Lapkovsky {jared-at-lv-em.com}
15, 33 -- To: microscopy-at-microscopy.com
15, 33 -- Content-Type: text/plain; charset=ISO-8859-1
15, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
15, 33 -- X-AntiAbuse: Primary Hostname - washington.serverhostcenter.com
15, 33 -- X-AntiAbuse: Original Domain - microscopy.com
15, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
15, 33 -- X-AntiAbuse: Sender Address Domain - lv-em.com
15, 33 -- X-Source:
15, 33 -- X-Source-Args:
15, 33 -- X-Source-Dir:
15, 33 -- Content-Transfer-Encoding: 8bit
15, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9QG09d9001998
==============================End of - Headers==============================




From: lcoons-at-memphis.edu
Date: Mon, 26 Oct 2009 13:42:19 -0500
Subject: [Microscopy] Job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The University of Memphis Director, Integrated Microscopy Center

Applications are invited for the Director of the Integrated Microscopy
Center (IMC) at the University of Memphis. This is a tenure-track
Assistant/Associate Professor position in the Department of Biology.
Applicants must have a Ph.D. or equivalent degree in the biological
sciences, and a solid record of peer-reviewed publications. Preference will
be given to those applicants with a history of an externally-funded research
program and mentoring of M.S. and Ph.D. students. We seek candidates with
broad expertise in microscopy, and a commitment to managing a multi-user
laboratory. The anticipated start date is August 2010.

The University of Memphis is a comprehensive state university with an
enrollment of approximately 21,000 students. The IMC is a fee for
service University wide facility that is also used by the surrounding
biomedical community. The IMC has confocal, light, atomic force and electron
microscopes with ancillary equipment and is staffed by skilled technicians.
The Director will be responsible for an operating budget and will supervise
the Center¹s activities. A complete description of the IMC can be found on
our web site given below. The Department of Biology offers B.S., M.S., and
Ph.D. degrees in Biology. There are approximately 30 faculty, 14 staff, 50
full-time graduate students, and over 750 majors in the department. The
department administers the Meeman Biological Field Station and the
Ecological Research Center, and is closely affiliated with the
interdisciplinary Bioinformatics Program and the W. Harry Feinstone Center
for Genomic Research.


Additional information: IMC information http://www.memphis.edu/microscopy
{http://www.memphis.edu/microscopy} ; Departmental information
http://biology.memphis.edu {http://biology.memphis.edu} ; University
information http://www.memphis.edu {http://www.memphis.edu} , or contact
Donna Haskins (search coordinator) (901) 678-2581 (dhaskins-at-memphis.edu).

Applicants should submit a letter of application, curriculum vitae, a
concise description of research and teaching interests and expertise in
microscopy and four letters of recommendation online at
https://workforum.memphis.edu

Women and minority candidates are encouraged to apply. The University of
Memphis is an Affirmative Action/Equal Opportunity Employer.



==============================Original Headers==============================
6, 26 -- From lcoons-at-memphis.edu Mon Oct 26 13:42:15 2009
6, 26 -- Received: from itmta2.memphis.edu (itmx2.memphis.edu [141.225.112.71])
6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9QIgC8R023812
6, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Oct 2009 13:42:13 -0500
6, 26 -- Received: from itexfe6.uom.memphis.edu (itexfe6.memphis.edu [10.15.1.67])
6, 26 -- by itmta2.memphis.edu (Postfix) with ESMTP id 86634A0C04C
6, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Oct 2009 13:42:11 -0500 (CDT)
6, 26 -- Received: from itexbe5.uom.memphis.edu ([10.15.1.64]) by
6, 26 -- itexfe6.uom.memphis.edu ([10.15.1.67]) with mapi; Mon, 26 Oct 2009 13:42:11
6, 26 -- -0500
6, 26 -- From: "Lewis B Coons (lcoons)" {lcoons-at-memphis.edu}
6, 26 -- To: "\"" {"} "-at-ns.microscopy.com}
6, 26 -- Date: Mon, 26 Oct 2009 13:42:10 -0500
6, 26 -- Subject: Job posting
6, 26 -- Thread-Topic: Job posting
6, 26 -- Thread-Index: AcpWbAaPAsfvAxZWa0+lkWJD94I8jQ==
6, 26 -- Message-ID: {C70B57B2.FDC1%lcoons-at-memphis.edu}
6, 26 -- Accept-Language: en-US
6, 26 -- Content-Language: en
6, 26 -- X-MS-Has-Attach:
6, 26 -- X-MS-TNEF-Correlator:
6, 26 -- acceptlanguage: en-US
6, 26 -- Content-Type: text/plain; charset="iso-8859-1"
6, 26 -- MIME-Version: 1.0
6, 26 -- Content-Transfer-Encoding: 8bit
6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9QIgC8R023812
==============================End of - Headers==============================




From: ramshes-at-musc.edu
Date: Mon, 26 Oct 2009 22:22:21 -0500
Subject: [Microscopy] viaWWW: Third Charleston Workshop on Light Microscopy for the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ramshes-at-musc.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ramshes-at-musc.edu
Name: Venkat Ramshesh

Organization: MUSC

Title-Subject: [Filtered] Third Charleston Workshop on Light
Microscopy for the Biosciences (LMB)

Question: Third Charleston Workshop on
LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Medical University of South Carolina
June 6-11, 2010
The Third Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES
(LMB) will provide a solid introduction to the concepts and practical
applications of light microscopy relevant to modern cell and
molecular biology. Students will have opportunities for extensive
hands-on experience with state-of-the-art equipment for optical
imaging, digital image processing, and fluorescence and
confocal/multiphoton microscopy guided by experienced academic and
commercial faculty. Lectures and laboratory exercises will include:
optics of image formation; microscope alignment; phase contrast and
differential interference contrast microscopy; video and digital
cameras; contrast enhancement by analog and digital image processing;
principles of fluorescence and fluorescence microscopy; ion imaging
and fluorescent probes, including green fluorescent protein;
fluorescence resonance energy transfer; and laser scanning confocal
and multiphoton microscopy. A commercial faculty representing leading
microscope manufacturers will make available for students use of the
latest and most advanced instrumentation for light microscopy, image
detection and computerized image analysis. The LMB Workshop is
designed for doctoral level scientists, advanced pre-doctoral
students and high level technical personnel. No prior experience with
microscopy is required. All students will benefit from in-depth
interaction with instructors. Students are encouraged to bring their
own specimens for analysis.

Tuition: $650.00
Application Deadline: April 1, 2010
Principal Instructors:
John J. Lemasters, M.D., Ph.D., Organizer
P. Darwin Bell, Ph.D.
Anna-Liisa Nieminen, Ph.D.
Venkat K. Ramshesh, Ph.D.

TO APPLY, send a curriculum vita and a brief letter describing
your research interests and reasons for enrolling. Because the course
is expected to be oversubscribed, applicants should inquire as soon
as possible. Please indicate your complete mailing address,
telephone/fax number and email address. Full consideration will be
given to applications received by April 1, 2010.

For further information and to apply, contact:
Venkat K. Ramshesh
Medical University of South Carolina
Center for Cell Death, Injury and Regeneration and Hollings Cancer Center
280 Calhoun Street, MSC 140
Charleston, SC 29425
Telephone (843) 792- 3530, FAX (843) 792-1617
E-mail: ramshes-at-musc.edu
Or visit http://academicdepartments.musc.edu/ccdir/events/workshop2010.htm


Login Host: 68.58.161.32
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Mon Oct 26 22:22:20 2009
10, 12 -- Received: from [206.69.208.22] ([130.202.238.72])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9R3MHkh024669
10, 12 -- for {microscopy-at-microscopy.com} ; Mon, 26 Oct 2009 22:22:19 -0500
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240800c70c17cf0496-at-[206.69.208.22]}
10, 12 -- Date: Mon, 26 Oct 2009 22:22:15 -0500
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: ramshes-at-musc.edu (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Third Charleston Workshop on Light Microscopy for the
10, 12 -- Biosciences (LMB)
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: y.chiu-at-bham.ac.uk
Date: Tue, 27 Oct 2009 08:49:13 -0500
Subject: [Microscopy] Looking for Second hand TEM holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We are looking for second hand double tilt TEM holders for Tecnai F20 and
JEM-2100 microscopes, if by any chance you have such second hand TEM holders
to offer, could you please contact me directly?


Best regards,

Yu Lung CHIU









==============================Original Headers==============================
13, 30 -- From y.chiu-at-bham.ac.uk Tue Oct 27 08:49:12 2009
13, 30 -- Received: from sun61.bham.ac.uk (sun61.bham.ac.uk [147.188.128.150])
13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9RDnCx8004170
13, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 08:49:12 -0500
13, 30 -- Received: from [147.188.128.127] (helo=bham.ac.uk)
13, 30 -- by sun61.bham.ac.uk with esmtp (Exim 4.67)
13, 30 -- (envelope-from {y.chiu-at-bham.ac.uk} )
13, 30 -- id 1N2lys-0001B7-Ni
13, 30 -- for Microscopy-at-microscopy.com; Tue, 27 Oct 2009 13:20:58 +0000
13, 30 -- Received: from exhub1.bham.ac.uk ([147.188.127.153] helo=EXHUB1.adf.bham.ac.uk)
13, 30 -- by bham.ac.uk with esmtp (Exim 4.43)
13, 30 -- id 1N2lys-0005mm-Dq
13, 30 -- for Microscopy-at-microscopy.com; Tue, 27 Oct 2009 13:20:58 +0000
13, 30 -- Received: from ex1.adf.bham.ac.uk (147.188.129.86) by EXHUB1.adf.bham.ac.uk
13, 30 -- (147.188.127.153) with Microsoft SMTP Server id 8.2.176.0; Tue, 27 Oct 2009
13, 30 -- 13:20:57 +0000
13, 30 -- Received: from [10.1.38.171] ([147.188.254.125]) by ex1.adf.bham.ac.uk with
13, 30 -- Microsoft SMTPSVC(6.0.3790.1830); Tue, 27 Oct 2009 13:20:57 +0000
13, 30 -- User-Agent: Microsoft-Entourage/11.4.0.080122
13, 30 -- Date: Tue, 27 Oct 2009 13:22:51 +0000
13, 30 -- Subject: Looking for Second hand TEM holders
13, 30 -- From: Yu Lung Chiu {y.chiu-at-bham.ac.uk}
13, 30 -- To: {Microscopy-at-microscopy.com}
13, 30 -- Message-ID: {C70CA4AB.A4C4%y.chiu-at-bham.ac.uk}
13, 30 -- Thread-Topic: Looking for Second hand TEM holders
13, 30 -- Thread-Index: AcpXCJVR1AE0mML7Ed6rxgAbY5Y/dA==
13, 30 -- MIME-Version: 1.0
13, 30 -- Content-Type: text/plain; charset="US-ASCII"
13, 30 -- Content-Transfer-Encoding: 7bit
13, 30 -- X-OriginalArrivalTime: 27 Oct 2009 13:20:57.0465 (UTC) FILETIME=[51A5B690:01CA5708]
==============================End of - Headers==============================




From: bnross-at-interchange.ubc.ca
Date: Tue, 27 Oct 2009 16:04:25 -0500
Subject: [Microscopy] Re: Zeiss EM10C focus/sample height issues.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Well good news, the Zeiss rep for our region stopped by to have a look at the scope and he fixed the problem we were having with the focus range.

To quote him:

"Just to clarify, the "not focusing" problem was due to a failed short
circuited capacitor in a power supply in the hi voltage. That caused an
extra approximate (but unknown)20KV boost to the Selected Hi Voltage, and
thus the Objective focusing lens had an incorrect reference voltage and was
out of focal range."

Thanks to everyone who sent in suggestions for things to check! I gained quite a bit of valuable information on how the scope works from those replies.

Thanks again,
-Brad

-----Original Message-----

} Date: Mon Oct 19 14:47:34 PDT 2009
} From: bnross-at-interchange.ubc.ca
} Subject: [Microscopy] Zeiss EM10C focus/sample height issues.
} To: bnross-at-interchange.ubc.ca
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello again,
}
} We donated our decommissioned Zeiss EM10C to another University about a year ago, and it has now been put back together and fired up for the first time since it was shipped. The physics prof who got the scope up and running managed to get a beam out of it, but that's pretty much where his expertise ended as far as I know. I took a trip up there over the weekend and managed to get the scope pretty well aligned, except that the sample only came into focus right at the very end of the course focus knob travel. Also, (I'm not sure if it's just the way these scopes work) when the standard focus knob is turned, it "resets" back to an initial focus step after a certain number of turns. For example, I could get the sample just to pretty good underfocus, and if I tried to keep turning the knob clockwise to go through focus to overfocus it would reset back to way under focus and repeat if I just kept turning the knob. I couldn't move to the next step on the course focus to correct fo
r!
} that because it was on the last clockwise stop.
}
} My first thought was that the sample height just needs to be adjusted, but I didn't see any obvious way of doing that on the column. Is there a nondescript knob or screw somewhere that allows adjustment of the sample height?
}
} My second thought was that there's something wrong with the objective lens current control(s) because of the weird "resetting" behaviour of the focus knob. Is there a way to check that? Could there be a cable connection that got missed or knocked loose in putting the scope back together after shipping?
}
} If it makes any difference, the scope was set up with STEM capability in the past, so it has the objective lens with the big Leybold ion pumps on the side. I tried inserting a sample with the STEM sample holder, but it didn't seem to fit, and for some reason caused the vacuum to leak rapidly when inserted. The vacuum holds steady with the standard holder.
}
} Also, the "Beam Alignment" knobs are not very clear in their function; I'm guessing one is beam shift or gun shift and the other is gun tilt or gun shift. Can anyone tell me exactly what each set of beam alignment knobs actually controls? Oh, and one more thing, is there a simple way to do C1 alignment, or does it involve allen wrenches and column screws as suggested by the manual?
}
} I'm not very familiar with Zeiss TEM's, so if anyone who owns or has owned/maintained one of these orange beasts could give some helpful hints, that would be great!
}
} Thanks,
} --
} Bradford Ross
}
} Microscopy Technician
} BioImaging Facility
} University of British Columbia
} 6270 University Blvd.
} Vancouver, B.C.
} Canada
} V6T 1Z4
}
} phone 604-822-6996
}
}
}
} ==============================Original Headers==============================
} 12, 28 -- From bnross-at-interchange.ubc.ca Mon Oct 19 16:40:40 2009
} 12, 28 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5])
} 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9JLeeDU021345
} 12, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 16:40:40 -0500
} 12, 28 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
} 12, 28 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id C4AA218163
} 12, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Oct 2009 14:40:38 -0700 (PDT)
} 12, 28 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
} 12, 28 -- by smtp.interchange.ubc.ca
} 12, 28 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
} 12, 28 -- with ESMTP id {0KRS007FQ6VPSS-at-smtp.interchange.ubc.ca} for
} 12, 28 -- Microscopy-at-microscopy.com; Mon, 19 Oct 2009 14:40:38 -0700 (PDT)
} 12, 28 -- Date: Mon, 19 Oct 2009 14:40:37 -0700 (PDT)
} 12, 28 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
} 12, 28 -- Subject: Zeiss EM10C focus/sample height issues.
} 12, 28 -- To: Microscopy-at-microscopy.com
} 12, 28 -- Message-id: {9850435.11511255988437255.JavaMail.myubc2-at-handel.my.ubc.ca}
} 12, 28 -- MIME-version: 1.0
} 12, 28 -- X-Mailer: uPortal WEB email client 3.0
} 12, 28 -- Content-type: text/plain; charset=us-ascii
} 12, 28 -- X-UBC-Scanned: Sophos PureMessage 5.5.6.374947, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.19.213037
} 12, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
} 12, 28 -- X-PerlMx-Spam: Probability=8%, Report=
} 12, 28 -- SUPERLONG_LINE 0.05, BODY_SIZE_2000_2999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0
} 12, 28 -- X-Spam-Level:
} 12, 28 -- X-Spam-Flag: No
} 12, 28 -- Content-Transfer-Encoding: 8bit
} 12, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9JLeeDU021345
} ==============================End of - Headers==============================
--
Bradford Ross

Microscopy Technician
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-6996


==============================Original Headers==============================
11, 27 -- From bnross-at-interchange.ubc.ca Tue Oct 27 16:04:24 2009
11, 27 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5])
11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9RL4OdG007719
11, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 16:04:24 -0500
11, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70])
11, 27 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id 1BA57182B8
11, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 14:04:17 -0700 (PDT)
11, 27 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14])
11, 27 -- by smtp.interchange.ubc.ca
11, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
11, 27 -- with ESMTP id {0KS6000AIYJ4ZT-at-smtp.interchange.ubc.ca} for
11, 27 -- Microscopy-at-microscopy.com; Tue, 27 Oct 2009 14:04:17 -0700 (PDT)
11, 27 -- Date: Tue, 27 Oct 2009 14:04:16 -0700 (PDT)
11, 27 -- From: Bradford Ross {bnross-at-interchange.ubc.ca}
11, 27 -- Subject: Re: [Microscopy] Zeiss EM10C focus/sample height issues.
11, 27 -- To: Microscopy-at-microscopy.com
11, 27 -- Message-id: {15336915.31901256677456933.JavaMail.myubc2-at-handel.my.ubc.ca}
11, 27 -- MIME-version: 1.0
11, 27 -- X-Mailer: uPortal WEB email client 3.0
11, 27 -- Content-type: text/plain; charset=us-ascii
11, 27 -- Content-transfer-encoding: 7bit
11, 27 -- X-UBC-Scanned: Sophos PureMessage 5.5.6.374947, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2009.10.27.205122
11, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
11, 27 -- X-PerlMx-Spam: Probability=8%, Report=
11, 27 -- SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __LINES_OF_YELLING 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __URI_NS
11, 27 -- X-Spam-Level:
11, 27 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: mazzillo-at-gmail.com
Date: Tue, 27 Oct 2009 20:45:10 -0500
Subject: [Microscopy] viaWWW: preparing unarmored dinoflagellates for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mazzillo-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mazzillo-at-gmail.com
Name: Fernanda Mazzillo

Organization: UCSC

Title-Subject: [Filtered] preparing unarmored dinoflagellates for TEM

Question: Hello

I am looking for someone or a lab that prepares samples for TEM. The
samples are of dinoflagellates infected with a parasite. I have a
protocol for the procedure but cannot executed it. I also have funds
to pay for the sample prep.

thank you very much
Fernanda

Login Host: 128.114.69.116
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Oct 27 20:45:10 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9S1j9de000321
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 20:45:10 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240802c70d5293cbc5-at-[206.69.208.22]}
8, 11 -- Date: Tue, 27 Oct 2009 20:45:09 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: mazzillo-at-gmail.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: preparing unarmored dinoflagellates for TEM
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rstallcup-at-zyvex.com
Date: Tue, 27 Oct 2009 23:17:34 -0500
Subject: [Microscopy] Hitachi s-4700 ExB detection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

Does anyone know how I can tell if my Hitachi s-4700 has ExB detection? The system has both upper and lower detectors. I don't see anything in the software interface to allow ExB mode. Is ExB a mode of operation that involves a combination of the lower detector grid voltage and the objective lens magnetic field?

Thanks,
Richard

--
........................................................................
Richard E. Stallcup II, PhD
Applications Manager
Zyvex Instruments, LLC

t: 972.792.1619
c: 972-522-9870
f: 972.235.7882
e: rstallcup-at-zyvex.com
w: www.zyvex.com

Sent via BlackBerry from T-Mobile

==============================Original Headers==============================
6, 22 -- From SRS0=lIINNl=GR=zyvex.com=rstallcup-at-srs.bis.na.blackberry.com Tue Oct 27 23:17:34 2009
6, 22 -- Received: from smtp02.bis.na.blackberry.com (smtp02.bis.na.blackberry.com [216.9.248.49])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9S4HWwb021352
6, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 23:17:33 -0500
6, 22 -- Received: from bda470.bisx.prod.on.blackberry (bda470.bisx.prod.on.blackberry [172.20.217.250])
6, 22 -- by srs.bis.na.blackberry.com (8.13.7 TEAMON/8.13.7) with ESMTP id n9S4HTS0023996
6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Oct 2009 04:17:29 GMT
6, 22 -- Received: from bda470.bisx.prod.on.blackberry (localhost.localdomain [127.0.0.1])
6, 22 -- by bda470.bisx.prod.on.blackberry (8.13.7 TEAMON/8.13.7) with ESMTP id n9S4HTN6017193
6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Oct 2009 04:17:29 GMT
6, 22 -- X-rim-org-msg-ref-id: 1577505120
6, 22 -- Message-ID: {1577505120-1256703448-cardhu_decombobulator_blackberry.rim.net-779996765--at-bda940.bisx.prod.on.blackberry}
6, 22 -- Reply-To: rstallcup-at-zyvex.com
6, 22 -- X-Priority: Normal
6, 22 -- Sensitivity: Normal
6, 22 -- Importance: Normal
6, 22 -- To: "Microscopy list serve" {Microscopy-at-microscopy.com}
6, 22 -- Subject: Hitachi s-4700 ExB detection
6, 22 -- From: "Richard Stallcup" {rstallcup-at-zyvex.com}
6, 22 -- Date: Wed, 28 Oct 2009 04:18:08 +0000
6, 22 -- Content-Type: text/plain
6, 22 -- MIME-Version: 1.0
==============================End of - Headers==============================




From: Alicia.Koek-at-csl.com.au
Date: Tue, 27 Oct 2009 23:56:32 -0500
Subject: [Microscopy] Reichert-Jung Knifemaker Manual/Instructions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have a Reichert-Jung Knifemaker which doesn't have any
manual/instructions and we were wondering if we could get a copy off
anyone?


Thanks,

Alicia Koek

Research Assistant

EM Unit - R&D Influenza Development [D.823]

CSL Limited


45 Poplar Road, Parkville | VIC 3052 | Australia

alicia.koek-at-csl.com.au

http://www.csl.com.au {http://www.csl.com.au/}





***************************************************************************************************************

This email and any attachments are confidential and may be subject to legal or other professional privilege. Any confidentiality or privilege is not waived or lost because this email has been sent to you by mistake. You should not read, copy, adapt, use or disclose them or their contents without authorisation. Any personal information in this email must be handled in accordance with the Privacy Act 1988 (Cth).
If you are not an intended recipient, please contact us at once by return email and then delete both messages.


CSL Limited A.C.N. 051 588 348
45 Poplar Road Parkville Victoria 3052 Australia
Phone: +61 3 9389 1911 Fax: +61 3 9389 1434
***************************************************************************************************************




==============================Original Headers==============================
3, 21 -- From prvs=155282755a=Alicia.Koek-at-csl.com.au Tue Oct 27 23:56:32 2009
3, 21 -- Received: from mail.csl.com.au (mail.csl.com.au [203.10.36.35])
3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9S4uVAT004710
3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2009 23:56:31 -0500
3, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 21 -- Content-class: urn:content-classes:message
3, 21 -- MIME-Version: 1.0
3, 21 -- Content-Type: text/plain; charset="us-ascii"
3, 21 -- Subject: Reichert-Jung Knifemaker Manual/Instructions
3, 21 -- Date: Wed, 28 Oct 2009 15:56:19 +1100
3, 21 -- Message-ID: {C45A048BC0F3694F8A8228E7EC40C44D09885F03-at-apaupkvmbx02.cslg1.cslg.net}
3, 21 -- X-MS-Has-Attach:
3, 21 -- X-MS-TNEF-Correlator:
3, 21 -- Thread-Topic: Reichert-Jung Knifemaker Manual/Instructions
3, 21 -- Thread-Index: AcpXiv0KiavUjpA6RkezEdzoQddpMg==
3, 21 -- From: {Alicia.Koek-at-csl.com.au}
3, 21 -- To: {Microscopy-at-microscopy.com}
3, 21 -- X-OriginalArrivalTime: 28 Oct 2009 04:56:20.0853 (UTC)
3, 21 -- FILETIME=[FDCB1A50:01CA578A]
3, 21 -- Content-Transfer-Encoding: 8bit
3, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9S4uVAT004710
==============================End of - Headers==============================




From: Lesley-at-mintek.co.za
Date: Wed, 28 Oct 2009 08:30:03 -0500
Subject: [Microscopy] viaWWW: EBSD camera problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Lesley-at-mintek.co.za as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Lesley-at-mintek.co.za
Name: Lesley Chown

Organization: Mintek

Title-Subject: [Filtered] EBSD camera problems

Question: We have a 2 year old Digiview camera with an EBSD system.
It has only been used for a few hours as it has broken down twice.
Apparently there was moisture inside the camera on the cooler kit
that caused the faults both times. Has anyone had similar problems?
Also, Can anyone advise me on what to do to prevent "moisture"
getting onto the camera (it is in an air-conditioned lab far from any
obvious water sources)?

Login Host: 196.35.158.178
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Oct 28 08:30:03 2009
6, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9SDU1sG018439
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2009 08:30:02 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c70df7ca859a-at-[206.69.208.22]}
6, 11 -- Date: Wed, 28 Oct 2009 08:30:00 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: Lesley-at-mintek.co.za (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: EBSD camera problems
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Wed, 28 Oct 2009 08:35:17 -0500
Subject: [Microscopy] Position for Localization and Light Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

======================================================
Please distribute or post this positon announcement wherever you can.
=======================================================


POSITION DESCRIPTION AND DUTIES - Microscopist

The Central Microscopy Facility at Miami University is seeking a full-
time staff member specializing in immunological and advanced
localization techniques for microscopy. This individual will take an
active role in teaching and research in wide-field light,
fluorescence and confocal microscopy including maintenance, training,
and consultation with users, and user support. The ability to support
image analysis is highly desired. Participation in research
collaborations is expected.

The successful applicant will join a growing team of microscopy
specialists in Miami University´s Central Microscopy Facility, and
will define and develop the roles of this position and future
directions of the Facility. The Microscopy Facility handles a
diversity of advanced microscopies including LM, Confocal, TEM, and
SEM for imaging biological and non-biological samples. The Facility
supports over 200 users annually from 12 different academic
departments, working directly with undergraduate and graduate
students, post-docs and faculty. The successful candidate will be
directly involved with testing and integration of new microscopy
systems as well as helping to identify and define future directions
for the facility. Additional information about the Microscopy
Facility can be found at www.emf.muohio.edu.


QUALIFICATION REQUIREMENTS

Master's or Ph.D. degree is preferred. Significant experience
working with biological imaging and sample preparation, as well as
experience working with a diversity of biological organisms (animals,
plants, fungi, etc.) is preferred. Additional skills in
ultramicrotomy, microscopy, computers and imaging skills are
desirable. Research experience and previous work in a teaching
environment are also desirable. The position offers opportunities for
training in advanced microscopy techniques including TEM, SEM and
cryo-preservation.

TO APPLY

Send a detailed cover letter, a CV, including recent publications, a
statement of any relevant research and teaching interests related to
microscopy, and have three letters of recommendation sent (preferably
via e-mail) to: Dr. Richard E. Edelmann, EM Facility Director, 364
Pearson Hall, Miami University, Oxford, OH 45056 Phone: 513-529-5712.
Email: EdelmaRE-at-muohio.edu


Miami University is an EOE/AA employer with smoke©free campuses.
Women, minorities, veterans, and persons with disabilities are
encouraged to apply. For information regarding campus crime and
safety, visit www.muohio.edu/righttoknow. Hard copy upon request.


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



==============================Original Headers==============================
16, 23 -- From edelmare-at-muohio.edu Wed Oct 28 08:35:16 2009
16, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.71])
16, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9SDZE6r025185
16, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 28 Oct 2009 08:35:16 -0500
16, 23 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
16, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n9SDZGOV032490
16, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 28 Oct 2009 09:35:16 -0400
16, 23 -- Received: from [10.34.160.234] ([10.34.160.234])
16, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n9SDZElc024804
16, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 28 Oct 2009 09:35:14 -0400
16, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
16, 23 -- To: microscopy-at-Microscopy.com
16, 23 -- Date: Wed, 28 Oct 2009 09:35:15 -0400
16, 23 -- MIME-Version: 1.0
16, 23 -- Subject: Position for Localization and Light Microscopist
16, 23 -- Message-ID: {4AE81053.24703.186FF8AF-at-edelmare.muohio.edu}
16, 23 -- Priority: normal
16, 23 -- X-mailer: Pegasus Mail for Windows (4.41)
16, 23 -- Content-type: text/plain; charset=ISO-8859-1
16, 23 -- Content-description: Mail message body
16, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.71
16, 23 -- Content-Transfer-Encoding: 8bit
16, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n9SDZE6r025185
==============================End of - Headers==============================




From: jason.gillen-at-encorewire.com
Date: Wed, 28 Oct 2009 18:14:52 -0500
Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jason.gillen-at-encorewire.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jason.gillen-at-encorewire.com
Name: Gillenium

Organization: Industry

Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??

Question: New lab comming online and need experienced information on
the "desktop" SEM units available now. I have read literature on
FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
also has a unit that offers X-ray analysis as well at a substantially
higher price ca. $200K USD. I have never used one of these
"benchtop" units and would appreciate any experience with them, who
makes the best one for the price, limitations etc. etc. Thanks

Login Host: 74.2.250.50
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Oct 28 18:14:52 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9SNEnoj016166
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2009 18:14:52 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c70e80caa215-at-[206.69.208.22]}
6, 11 -- Date: Wed, 28 Oct 2009 18:14:48 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: jason.gillen-at-encorewire.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Benchtop SEM, biggest bang for buck??
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: larry.ackerman-at-ucsf.edu
Date: Thu, 29 Oct 2009 12:49:17 -0500
Subject: [Microscopy] Biology TEM vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

EsB mode is method of collecting Backscattered Electrons through the lens.
This method requires a "BSE detector" above the final lens and to be
successful a grid between the specimen and the detector to exclude SE from
the signal. Whilst the very latest instruments incorporate a version of
this technique it was not available (invented?) when the 4700 was in
production.

You may note "BSE Detector" as the detector does not conform to "normal" BSE
detecting practice, but very interesting!

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {rstallcup-at-zyvex.com}
To: {protrain-at-emcourses.com}
Sent: Wednesday, October 28, 2009 4:19 AM



The ExB detector sits directly on top of the EDS port on the left side of the
column. You will notice a small ~1" x 1" cube with a yellow triangular high
voltage warning sticker. You can also tell if you have ExB by looking at the
image display window. At the bottom right-hand side you will see a "signal
control" tab (next to the "cell count" tab). Clicking on this will open to a
radio button option with two choices - "Se" or "Se/Bse", if you select the
Se/Bse button you can then access the ExB filter slider control which allows
you to dial in the best quality image, assuming you have this option, which
became available on the S-4700's at some point towards the middle/end of
2002.


John Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Ridgebury Rd. / PO Box 368
Ridgefield, CT 06877
Phone (203)798-5640



-----Original Message-----
X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com]
Sent: Thursday, October 29, 2009 10:16 AM
To: Robson,John AN BIP-US-R

Hi

EsB mode is method of collecting Backscattered Electrons through the lens.
This method requires a "BSE detector" above the final lens and to be
successful a grid between the specimen and the detector to exclude SE from
the signal. Whilst the very latest instruments incorporate a version of
this technique it was not available (invented?) when the 4700 was in
production.

You may note "BSE Detector" as the detector does not conform to "normal" BSE
detecting practice, but very interesting!

Steve

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com


----- Original Message -----
X-from: {rstallcup-at-zyvex.com}
To: {protrain-at-emcourses.com}
Sent: Wednesday, October 28, 2009 4:19 AM

I also would like to know about the performance of these bench-top SEM
instruments and how they compare with full-size ones. And, to have some
orientation on its price. I know actual quotations would depend on many
different factors and can differ from lab to lab, but just to get an idea of
which level of funds has one to get to afford them.

Many thanks,

Antonio D. Molina-Garcia

Instituto del Frío (CSIC)
José Antonio Nováis, 10
Ciudad Universitaria
28040 Madrid
España

Tel +34 915445607 Fax +34 915493627 E-mail : ifrm111-at-if.csic.es
http://www.if.csic.es/ingiind.htm


----- Original Message -----
X-from: {jason.gillen-at-encorewire.com}
To: {ifrm111-at-if.csic.es}
Sent: Thursday, October 29, 2009 12:15 AM

The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).

The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.

It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.

It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.

Hope this helps.

Zhaojie


Zhaojie Zhang, Ph.D.
Director, Microscopy Core Facility
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071
zzhang-at-uwyo.edu
307-766-3038




-----Original Message-----
X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
Sent: Thursday, October 29, 2009 11:14 AM
To: Z.J. Zhang

I also would like to know about the performance of these bench-top SEM
instruments and how they compare with full-size ones. And, to have some
orientation on its price. I know actual quotations would depend on many
different factors and can differ from lab to lab, but just to get an idea of
which level of funds has one to get to afford them.

Many thanks,

Antonio D. Molina-Garcia

Instituto del Frío (CSIC)
José Antonio Nováis, 10
Ciudad Universitaria
28040 Madrid
España

Tel +34 915445607 Fax +34 915493627 E-mail : ifrm111-at-if.csic.es
http://www.if.csic.es/ingiind.htm


----- Original Message -----
X-from: {jason.gillen-at-encorewire.com}
To: {ifrm111-at-if.csic.es}
Sent: Thursday, October 29, 2009 12:15 AM

Dear Listers,
We are having some discussions regarding the most appropriate vacuum
system for a 120kV LaB6 TEM dedicated to biology samples--ultrathin
epoxy embedded sections at magnifications less than 100k and a digital
camera (no photographic film).

The choice is between a cascade oil diffusion pumped system achieving 4
X 10-5 Pa and a turbo molecular pumped system.

Two points in favor of the DP system:
1) It recovers rapidly after specimen insertion (15 second turnaround)
and is more forgiving with beginning users. We have grad student and
post-doc users.
2) The TMP significantly increases the price of the service contract.

Do you have any recommendations based on your experience?

Thanks,
Larry




--
Larry Ackerman, Specialist
Electron Microscopy Lab
UCSF, Dept. of Anatomy, Rm S1355
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400

==============================Original Headers==============================
11, 30 -- From Larry.Ackerman-at-ucsf.edu Thu Oct 29 12:49:16 2009
11, 30 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214])
11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9THnFII003466
11, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:49:16 -0500
11, 30 -- X-ASG-Debug-ID: 1256838553-10d8025a0000-1DjkGe
11, 30 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
11, 30 -- Received: from EXHT02.net.ucsf.edu (localhost [127.0.0.1])
11, 30 -- by bcuda2.ucsf.edu (Spam & Virus Firewall) with ESMTP id 90C7D48D304
11, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 10:49:13 -0700 (PDT)
11, 30 -- Received: from EXHT02.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda2.ucsf.edu with ESMTP id wOWrFFpOYT7uKRG9 for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 10:49:13 -0700 (PDT)
11, 30 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
11, 30 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
11, 30 -- Received: from Ralston-Lab-iMac.local (128.218.123.106) by EXHT02.net.ucsf.edu
11, 30 -- (64.54.247.219) with Microsoft SMTP Server id 8.1.393.1; Thu, 29 Oct 2009
11, 30 -- 10:49:12 -0700
11, 30 -- Message-ID: {4AE9D595.4080407-at-ucsf.edu}
11, 30 -- Date: Thu, 29 Oct 2009 10:49:09 -0700
11, 30 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
11, 30 -- Reply-To: larry.ackerman-at-ucsf.edu
11, 30 -- Organization: UCSF, NeuroAnatomy
11, 30 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
11, 30 -- MIME-Version: 1.0
11, 30 -- To: {Microscopy-at-microscopy.com}
11, 30 -- X-ASG-Orig-Subj: Biology TEM vacuum systems
11, 30 -- Subject: Biology TEM vacuum systems
11, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
11, 30 -- Content-Transfer-Encoding: 7bit
11, 30 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
11, 30 -- X-Barracuda-Start-Time: 1256838553
11, 30 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at ucsf.edu
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 29 Oct 2009 13:41:04 -0500
Subject: [Microscopy] Biology TEM vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Larry,
The service contract price tells a lot. When there is a major problem with
a DP, you just clean it, add new oil and away you go (except for the
occasional heater). Even using exotic oils, you're probably well under $1k
for materials and you can do it yourself. There is essentially no routine
maintenance required.

A TMP absolutely requires regular maintenance and when (not if) anything
goes wrong you're in for at least $2.5k and you can't fix it yourself, in
most cases. These are not forgiving pumps. Power consumption is low,
though, and water cooling may not be necessary.

As to which is cleaner, it all depends... There are lots of variables and
either one can come out as cleaner or dirtier. I've seen mighty clean DP
pumped systems and pretty filthy TMP pumped systems. I've also seen the
reverse.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu]
Sent: Thursday, October 29, 2009 1:51 PM
To: kenconverse-at-qualityimages.biz

Dear Listers,
We are having some discussions regarding the most appropriate vacuum
system for a 120kV LaB6 TEM dedicated to biology samples--ultrathin
epoxy embedded sections at magnifications less than 100k and a digital
camera (no photographic film).

The choice is between a cascade oil diffusion pumped system achieving 4
X 10-5 Pa and a turbo molecular pumped system.

Two points in favor of the DP system:
1) It recovers rapidly after specimen insertion (15 second turnaround)
and is more forgiving with beginning users. We have grad student and
post-doc users.
2) The TMP significantly increases the price of the service contract.

Do you have any recommendations based on your experience?

Thanks,
Larry




--
Larry Ackerman, Specialist
Electron Microscopy Lab
UCSF, Dept. of Anatomy, Rm S1355
513 Parnassus Ave., Box 0452
San Francisco, CA 94143

larry.ackerman-at-ucsf.edu

415-476-4400

==============================Original Headers==============================
11, 30 -- From Larry.Ackerman-at-ucsf.edu Thu Oct 29 12:49:16 2009
11, 30 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214])
11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
n9THnFII003466
11, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:49:16
-0500
11, 30 -- X-ASG-Debug-ID: 1256838553-10d8025a0000-1DjkGe
11, 30 -- X-Barracuda-URL: http://cuda.ucsf.edu:80/cgi-bin/mark.cgi
11, 30 -- Received: from EXHT02.net.ucsf.edu (localhost [127.0.0.1])
11, 30 -- by bcuda2.ucsf.edu (Spam & Virus Firewall) with ESMTP id
90C7D48D304
11, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 10:49:13
-0700 (PDT)
11, 30 -- Received: from EXHT02.net.ucsf.edu (mx.ucsf.edu [64.54.247.193])
by bcuda2.ucsf.edu with ESMTP id wOWrFFpOYT7uKRG9 for
{Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 10:49:13 -0700 (PDT)
11, 30 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu
11, 30 -- X-Barracuda-RBL-Trusted-Forwarder: 64.54.247.193
11, 30 -- Received: from Ralston-Lab-iMac.local (128.218.123.106) by
EXHT02.net.ucsf.edu
11, 30 -- (64.54.247.219) with Microsoft SMTP Server id 8.1.393.1; Thu, 29
Oct 2009
11, 30 -- 10:49:12 -0700
11, 30 -- Message-ID: {4AE9D595.4080407-at-ucsf.edu}
11, 30 -- Date: Thu, 29 Oct 2009 10:49:09 -0700
11, 30 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu}
11, 30 -- Reply-To: larry.ackerman-at-ucsf.edu
11, 30 -- Organization: UCSF, NeuroAnatomy
11, 30 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
11, 30 -- MIME-Version: 1.0
11, 30 -- To: {Microscopy-at-microscopy.com}
11, 30 -- X-ASG-Orig-Subj: Biology TEM vacuum systems
11, 30 -- Subject: Biology TEM vacuum systems
11, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
11, 30 -- Content-Transfer-Encoding: 7bit
11, 30 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193]
11, 30 -- X-Barracuda-Start-Time: 1256838553
11, 30 -- X-Barracuda-Virus-Scanned: by Mail-at-UCSF Spam Firewall at
ucsf.edu
==============================End of - Headers==============================




==============================Original Headers==============================
25, 25 -- From kenconverse-at-qualityimages.biz Thu Oct 29 13:41:04 2009
25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TIf3AF025651
25, 25 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 13:41:04 -0500
25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
25, 25 -- with ESMTP
25, 25 -- id {20091029184102340.YBID9287-at-cdptpa-omta02.mail.rr.com} ;
25, 25 -- Thu, 29 Oct 2009 18:41:02 +0000
25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 25 -- To: {larry.ackerman-at-ucsf.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
25, 25 -- Subject: RE: [Microscopy] Biology TEM vacuum systems
25, 25 -- Date: Thu, 29 Oct 2009 14:40:53 -0400
25, 25 -- Message-ID: {3129E6C71BC84398BCF80654483F9C41-at-Ken}
25, 25 -- MIME-Version: 1.0
25, 25 -- Content-Type: text/plain;
25, 25 -- charset="us-ascii"
25, 25 -- X-Priority: 3 (Normal)
25, 25 -- X-MSMail-Priority: Normal
25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
25, 25 -- thread-index: AcpYwGx0IExme2YoQWqTNaCS3vZm6QABQwsg
25, 25 -- Importance: Normal
25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
25, 25 -- In-Reply-To: {200910291751.n9THpIor010634-at-ns.microscopy.com}
25, 25 -- Content-Transfer-Encoding: 8bit
25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9TIf3AF025651
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Thu, 29 Oct 2009 14:27:40 -0500
Subject: [Microscopy] Biology TEM vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Larry,

No question about it: for your system, a DP is the way to go. Having
said that, if you have in mind to do cryo work at some future time,
then possibly consider TMP. But, as was pointed out, it is an
extremely expensive proposition throughout. Remember, too, if one ever
fails out of contract, you're looking at $25-30K in repairs.

Our newest biological TEM is DP, even though we could have had a TMP.
Service contracts are often 30-50% less, as well.

John Bozzola
Carbondale, IL

==============================Original Headers==============================
4, 17 -- From bozzola-at-siu.edu Thu Oct 29 14:27:40 2009
4, 17 -- Received: from mail-pw0-f51.google.com (mail-pw0-f51.google.com [209.85.160.51])
4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TJRdF2011043
4, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 14:27:39 -0500
4, 17 -- Received: by pwi19 with SMTP id 19so251162pwi.10
4, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:27:39 -0700 (PDT)
4, 17 -- MIME-Version: 1.0
4, 17 -- Received: by 10.114.237.18 with SMTP id k18mr337953wah.63.1256844459089; Thu,
4, 17 -- 29 Oct 2009 12:27:39 -0700 (PDT)
4, 17 -- In-Reply-To: {200910291841.n9TIftxK026926-at-ns.microscopy.com}
4, 17 -- References: {200910291841.n9TIftxK026926-at-ns.microscopy.com}
4, 17 -- Date: Thu, 29 Oct 2009 14:27:39 -0500
4, 17 -- Message-ID: {ebc2299e0910291227s54c8ac86ra3420f40639e170f-at-mail.gmail.com}
4, 17 -- Subject: Re: [Microscopy] RE: Biology TEM vacuum systems
4, 17 -- From: John Bozzola {bozzola-at-siu.edu}
4, 17 -- To: MSAListserver {Microscopy-at-microscopy.com}
4, 17 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Thu, 29 Oct 2009 16:02:01 -0500
Subject: [Microscopy] RE; Pump for Bio TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with others that an oil diffusion pump would probably be the
best choice for Larry Ackerman's TEM.. While a turbomolecular pump
will give a cleaner vacuum, specimen contamination does not sound as
though it swill be a major problem for the type of specimens and the
magnification range he says he will be using. However, oil diffusion
pumps are subject to many problems that can cause very serious
consequences for an electron microscope. In one incident that I very
well remember the failure of a diffusion pump system resulted in such
a high concentration of oil vapors entering the column that the
window on the viewing chamber was covered with such a thick coating
of oil that we couldn't see through it. Cleaning the column after
this event was a tremendously difficult task, and it is unlikely that
we really got all the oil out. Anyone not thoroughly familiar with
the characteristics and operational requirements of this type of pump
would be well advised to review the discussion of them in Chapter 5
and Section 9.2 of "Vacuum Methods in Electron Microscopy" (ISBN 1
85578052)
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
1, 15 -- From bigelow-at-umich.edu Thu Oct 29 16:02:01 2009
1, 15 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161])
1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TL21mw030588
1, 15 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 16:02:01 -0500
1, 15 -- Received: FROM [99.130.39.221] (adsl-99-130-39-221.dsl.sfldmi.sbcglobal.net [99.130.39.221])
1, 15 -- By tombraider.mr.itd.umich.edu ID 4AEA02C8.2E3F2.15715 ;
1, 15 -- Authuser bigelow;
1, 15 -- 29 Oct 2009 17:02:00 EDT
1, 15 -- Mime-Version: 1.0
1, 15 -- Message-Id: {p06240801c70fab0efd76-at-[99.130.39.221]}
1, 15 -- Date: Thu, 29 Oct 2009 17:01:52 -0400
1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 15 -- Subject: [Microscopy]RE; Pump for Bio TEM
1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Thu, 29 Oct 2009 16:15:34 -0500
Subject: [Microscopy] Re: RE; Pump for Bio TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good point Dr. Bigelow makes about DP vacuum disasters.

In the modern instrument, with automatic valving, this "should" not
happen. If it does, that's when a service contract more than pays for
itself, believe me.

--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901

==============================Original Headers==============================
3, 17 -- From bozzola-at-siu.edu Thu Oct 29 16:15:33 2009
3, 17 -- Received: from mail-px0-f188.google.com (mail-px0-f188.google.com [209.85.216.188])
3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TLFXnT013048
3, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 16:15:33 -0500
3, 17 -- Received: by pxi26 with SMTP id 26so1543369pxi.22
3, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 14:15:33 -0700 (PDT)
3, 17 -- MIME-Version: 1.0
3, 17 -- Received: by 10.114.87.5 with SMTP id k5mr391718wab.145.1256850933146; Thu, 29
3, 17 -- Oct 2009 14:15:33 -0700 (PDT)
3, 17 -- In-Reply-To: {200910292102.n9TL2nkd031881-at-ns.microscopy.com}
3, 17 -- References: {200910292102.n9TL2nkd031881-at-ns.microscopy.com}
3, 17 -- Date: Thu, 29 Oct 2009 16:15:33 -0500
3, 17 -- Message-ID: {ebc2299e0910291415o76875964s7720e871e86085bf-at-mail.gmail.com}
3, 17 -- Subject: Re: [Microscopy] RE; Pump for Bio TEM
3, 17 -- From: John Bozzola {bozzola-at-siu.edu}
3, 17 -- To: MSAListserver {Microscopy-at-microscopy.com}
3, 17 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Thu, 29 Oct 2009 16:30:44 -0500
Subject: [Microscopy] Biology TEM vacuum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Having experience since almost 20 years with one particular LaB6 TEM of one manufacturer, which has been used almost exclusively for "biological" TEM, here is a brief comment / summary:

this TEM has worked absolutely reliably for almost all of the time. (no DP desaster ... I keep my fingers crossed ... ) The TEM was and is ON about 355 to 360 days a year, and it has: a rotary pump; a DP (which is great, cheap, reliable, appropriate); an IGP, which is an absolut must, in my opinion, if you want to operate the LaB6 (usually, one LaB6 is at least 3 years in - heavy - operation; with, on average, always more than or at least 10 to 15 different operators; including teaching; users: undergrads, phD students, postdocs; average 40 (30 - 55) hrs per week, with sometimes frequent, sometimes rare specimen changes, depending on the user). -- We always have the LN2 trap filled - always. And, an additional "cryo-finger" in the objective lens part, which was nice and useful for occasional cryo-work.
(secret? none. Regular supervision, regular and tight teaching and supervision. Regular maintenance, once or twice a year; second IGP after about 15 years, only)
Work: sections, freeze-etched samples, negative staining, as usual for biologists. 2 years included cryo - no vacuum problems.

concerning the question from Larry:
For sure, if I had to decide, I would choose an identical or at least similar machine, under similar conditions. - this means, not only the DP matters, but also the logic of the vacuum control, the valves, the IGP (for the LaB6), the LN2 trap, and if you can afford it, the cryo-finger.

HTH - kind regards - Reinhard Rachel


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
9, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Oct 29 16:30:44 2009
9, 23 -- Received: from rrzmta5.rz.uni-regensburg.de (rrzmta5.rz.uni-regensburg.de [194.94.155.56])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TLUhNh027903
9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 16:30:44 -0500
9, 23 -- Received: from rrzmta5.rz.uni-regensburg.de (localhost [127.0.0.1])
9, 23 -- by localhost (Postfix) with SMTP id 58321B70
9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 22:30:40 +0100 (CET)
9, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
9, 23 -- by rrzmta5.rz.uni-regensburg.de (Postfix) with ESMTP id 074E7A91
9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 22:30:39 +0100 (CET)
9, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
9, 23 -- with Novell_GroupWise; Thu, 29 Oct 2009 22:30:40 +0100
9, 23 -- Message-Id: {4AEA178C020000540000B08C-at-gwsmtp1.uni-regensburg.de}
9, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.1
9, 23 -- Date: Thu, 29 Oct 2009 22:30:36 +0100
9, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
9, 23 -- To: "microscopy listserver" {Microscopy-at-microscopy.com}
9, 23 -- Subject: Biology TEM vacuum
9, 23 -- Mime-Version: 1.0
9, 23 -- Content-Type: text/plain; charset=US-ASCII
9, 23 -- Content-Disposition: inline
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9TLUhNh027903
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Thu, 29 Oct 2009 16:55:59 -0500
Subject: [Microscopy] viaWWW: Stopping Times Allowed for Processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Stopping Times Allowed for Processing

Question: This question was asked earlier in the year, but my
question is slightly different.

I have a group here who might ultimately want EM done on their
samples, but have to wait until another study is complete.

It is a pretty large study, lots of samples, but before we really get
going with the TEM sample prep they want to make sure their
biochemical assay gives them the results they hope.

So my question to the group is, can we just fix them using our
primary fixative step and then rinse in buffer and perhaps leave them
this way, for maybe as long as two weeks waiting for the biochemistry
results?

We would keep them in 4C and I use sodium cacodylate buffer. The
tissue will be from mice.

The question that was asked back in February was in regards to what
steps you could stop at for an overnight break. My question is a bit
more extreme in that I want to wait for a much longer time.

Thank you all for your help.

Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher-at-princeton.edu

Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Thu Oct 29 16:55:59 2009
13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9TLtvf4010945
13, 11 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 16:55:58 -0500
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240800c70fbfc265ba-at-[206.69.208.22]}
13, 11 -- Date: Thu, 29 Oct 2009 16:55:55 -0500
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: Stopping Times Allowed for Processing
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 29 Oct 2009 20:24:43 -0500
Subject: [Microscopy] Re: viaWWW: EBSD camera problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oil diffusion pump works best in this case. But ODP (or turbo for that
matter) alone is enough for reliable operation of a LaB6 cathode. Ion pump
is a must.

LaB6 is impractical at pressures above 1E-6 Torr. TMP and ODP both capable
of demonstrating better ultimate vacuum - in your example tandem ODP will
achieve 4E-5 Pa which is approx. 3E-7 Torr. True for a brand new instrument
and about 3 times better than least acceptable. It is however ultimate
vacuum - so called because pumping speed slows down near equilibrium between
pumping capacity and leaks/ back pressure, becoming zero at ultimate vacuum.
Leave TEM alone for 10 hours and observe ultimate vacuum. But forget about
ultimate vacuum during work session with specimen exchange every 20 minutes.

IGP on the other hand capable of ultimate vacuum of at least E-10 Torr. Or,
in a realistic TEM column pumping application E-7 Torr or slightly better.

LaB6 could ideally approach 10,000 beam hours in a perfect environment with
diligent user and TEM in perfect condition. Or, it must last at least 3,000
hours in realistic multi-user environment. If less - than either user or an
instrument needs attention. The oldest LaB6 cathode that I know still in
operation is 12 years old (installed in 1997) with at least 6,000 hours
logged.


Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

----- Original Message -----
X-from: {larry.ackerman-at-ucsf.edu}
To: {vitalylazar-at-att.net}
Sent: Thursday, October 29, 2009 1:50 PM

How old is the system?

Mine is about four years old and I have never had any
problems with the camera. The FSD feature is rather
useless but other than that, the system is very stable.

Is the camera a 64xx (6412)? These seem to be very solid.
My suggestion is to get with EDAX and then remove the EBSD
unit from the chamber, close the port opening and send
in the camera with lens.

Alternatively, if someone bashed the phosphor screen, that
would compromise the patterns. Look also to see if you have
the old pair of FSD BSE diodes. If they are damaged, that
can affect your results. Someday, I will remove the FSD
diodes since the function is not all that effective for
the specimens I deal with.

Does the unit have SS bellows into the chamber? If this leaks,
there will be problems.

gary g.


At 06:31 AM 10/28/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
12, 20 -- From gary-at-gaugler.com Thu Oct 29 20:24:43 2009
12, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n9U1OgpG018277
12, 20 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 20:24:43 -0500
12, 20 -- Message-Id: {200910300124.n9U1OgpG018277-at-ns.microscopy.com}
12, 20 -- Received: (qmail 6067 invoked from network); 29 Oct 2009 18:05:11 -0700
12, 20 -- Received: by simscan 1.1.0 ppid: 6064, pid: 6065, t: 0.1050s
12, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
12, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
12, 20 -- by smtp2 with SMTP; 29 Oct 2009 18:05:11 -0700
12, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 20 -- Date: Thu, 29 Oct 2009 18:24:38 -0700
12, 20 -- To: Lesley-at-mintek.co.za
12, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
12, 20 -- Subject: Re: [Microscopy] viaWWW: EBSD camera problems
12, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
12, 20 -- In-Reply-To: {200910281331.n9SDVbkV020781-at-ns.microscopy.com}
12, 20 -- References: {200910281331.n9SDVbkV020781-at-ns.microscopy.com}
12, 20 -- Mime-Version: 1.0
12, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: cw23-at-ohm.york.ac.uk
Date: Fri, 30 Oct 2009 04:37:09 -0500
Subject: [Microscopy] Re: Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Field emission sources provide better spatial resolution than
traditional thermionic sources and
I believe Novelx is the only company offering a benchtop Field Emission SEM.

Chris Walker
Department of Electronics
University of York,
Heslington,
York,
YO10 5DD.

ZZhang-at-uwyo.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).
}
} The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.
}
} It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.
}
} It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.
}
} Hope this helps.
}
} Zhaojie
}
}
} Zhaojie Zhang, Ph.D.
} Director, Microscopy Core Facility
} Department of Zoology and Physiology
} University of Wyoming
} Laramie, WY 82071
} zzhang-at-uwyo.edu
} 307-766-3038
}
}
}
}
} -----Original Message-----
} X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
} Sent: Thursday, October 29, 2009 11:14 AM
} To: Z.J. Zhang
} Subject: [Microscopy] Re: viaWWW: Benchtop SEM, biggest bang for buck??
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I also would like to know about the performance of these bench-top SEM
} instruments and how they compare with full-size ones. And, to have some
} orientation on its price. I know actual quotations would depend on many
} different factors and can differ from lab to lab, but just to get an idea of
} which level of funds has one to get to afford them.
}
} Many thanks,
}
} Antonio D. Molina-Garcia
}
} Instituto del Frío (CSIC)
} José Antonio Nováis, 10
} Ciudad Universitaria
} 28040 Madrid
} España
}
} Tel +34 915445607 Fax +34 915493627 E-mail : ifrm111-at-if.csic.es
} http://www.if.csic.es/ingiind.htm
}
}
} ----- Original Message -----
} X-from: {jason.gillen-at-encorewire.com}
} To: {ifrm111-at-if.csic.es}
} Sent: Thursday, October 29, 2009 12:15 AM
} Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??
}
}
}
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} } ---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when
} } replying
} } please copy both jason.gillen-at-encorewire.com as well as the
} } MIcroscopy Listserver
} } ---------------------------------------------------------------------------
} }
} } Email: jason.gillen-at-encorewire.com
} } Name: Gillenium
} }
} } Organization: Industry
} }
} } Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??
} }
} } Question: New lab comming online and need experienced information on
} } the "desktop" SEM units available now. I have read literature on
} } FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
} } also has a unit that offers X-ray analysis as well at a substantially
} } higher price ca. $200K USD. I have never used one of these
} } "benchtop" units and would appreciate any experience with them, who
} } makes the best one for the price, limitations etc. etc. Thanks
} }
} }
}
}
} ==============================Original Headers==============================
} 26, 29 -- From ZZhang-at-uwyo.edu Thu Oct 29 12:44:43 2009
} 26, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
} 26, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9THih0Z027206
} 26, 29 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:44:43 -0500
} 26, 29 -- Received: from ponyexpress-ht2.uwyo.edu (ponyexpress-ht2.uwyo.edu [10.84.60.209])
} 26, 29 -- by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n9THiZp0024405
} 26, 29 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
} 26, 29 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 11:44:41 -0600 (MDT)
} 26, 29 -- (envelope-from ZZhang-at-uwyo.edu)
} 26, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht2
} 26, 29 -- ([10.84.60.209]) with mapi; Thu, 29 Oct 2009 11:44:41 -0600
} 26, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
} 26, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 26, 29 -- Date: Thu, 29 Oct 2009 11:44:45 -0600
} 26, 29 -- Subject: RE: Benchtop SEM, biggest bang for buck??
} 26, 29 -- Thread-Topic: Benchtop SEM, biggest bang for buck??
} 26, 29 -- Thread-Index: AcpYuyw8wl6LJJ/ZShir1Dy+wAR+bgAARZMw
} 26, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE95525A14-at-ponyexpress-mb2}
} 26, 29 -- References: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} 26, 29 -- In-Reply-To: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} 26, 29 -- Accept-Language: en-US
} 26, 29 -- Content-Language: en-US
} 26, 29 -- X-MS-Has-Attach:
} 26, 29 -- X-MS-TNEF-Correlator:
} 26, 29 -- acceptlanguage: en-US
} 26, 29 -- Content-Type: text/plain; charset="iso-8859-1"
} 26, 29 -- MIME-Version: 1.0
} 26, 29 -- Content-Transfer-Encoding: 8bit
} 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9THih0Z027206
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
4, 27 -- From cw23-at-ohm.york.ac.uk Fri Oct 30 04:37:09 2009
4, 27 -- Received: from mail.elec.york.ac.uk (mailgw0.elec.york.ac.uk [144.32.152.4])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9U9b84u024599
4, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Oct 2009 04:37:08 -0500
4, 27 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
4, 27 -- by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
4, 27 -- id 1N3nut-0005xF-BL
4, 27 -- for Microscopy-at-Microscopy.Com; Fri, 30 Oct 2009 09:37:07 +0000
4, 27 -- Message-ID: {4AEAB3B8.5050807-at-ohm.york.ac.uk}
4, 27 -- Date: Fri, 30 Oct 2009 09:36:56 +0000
4, 27 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
4, 27 -- Organization: Department of Electronics, The University of York
4, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
4, 27 -- MIME-Version: 1.0
4, 27 -- To: Microscopy-at-Microscopy.Com
4, 27 -- Subject: Re: Benchtop SEMs
4, 27 -- References: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
4, 27 -- In-Reply-To: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 27 -- Content-Transfer-Encoding: 8bit
4, 27 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
4, 27 -- X-YorkElec-MailScanner-ID: 1N3nut-0005xF-BL
4, 27 -- X-YorkElec-MailScanner: Found to be clean
4, 27 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
4, 27 -- X-YorkElec-MailScanner-Watermark: 1257500227.4277-at-qh3QwG3i4HoZQ9oz8IaMWg
4, 27 -- X-Spam-Status: No
4, 27 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
==============================End of - Headers==============================




From: avergason-at-tetracore.com
Date: Fri, 30 Oct 2009 08:28:06 -0500
Subject: [Microscopy] viaWWW:CM-10 Communications Expansion Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both avergason-at-tetracore.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: avergason-at-tetracore.com
Name: Amy Vergason

Organization: Tetracore, Inc

Title-Subject: [Filtered] Communications Expansion Board

Question: Hi everyone,

I posted a question last week looking fir a Quad Serial Interface
Board for a Phillips CM-10 TEM. First of all, thank you to all who
helped, I actually did find one! Second, I need another part to make
it work... It is a Communications Expansion Board. I have a picture
of it but do not know how to attach it to this email. If anyone out
there thinks they may have one or know where I can get one, I will
email the pic to you to be sure!!!

Thanks for all your help!
Amy

Login Host: 66.3.246.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Oct 30 08:28:06 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UDS5SL017556
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 08:28:05 -0500
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240805c7109a4f9fc6-at-[206.69.208.22]}
8, 11 -- Date: Fri, 30 Oct 2009 08:28:03 -0500
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: avergason-at-tetracore.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW:CM-10 Communications Expansion Board
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cw23-at-ohm.york.ac.uk
Date: Fri, 30 Oct 2009 11:39:14 -0500
Subject: [Microscopy] Re: Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I get the impression that the Novel-X mySEM is fundamentally different that 'normal' SEM's.

It seems to be limited in working distance. Its maximum voltage is listed as 2 kV, so those factors pretty well rule out EDS.

The maximum scan field is listed as 1x1 mm with a low magnification limit of 250x. I often bump up against our SEM's 25x low mag limit, so the mySEM would not be practical for me.

Undoubtedly it will find some useful niches. Just make sure it is suited to your needs.

Warren S.


-----Original Message-----
X-from: cw23-at-ohm.york.ac.uk [mailto:cw23-at-ohm.york.ac.uk]
Sent: Friday, October 30, 2009 4:38 AM
To: wesaia-at-iastate.edu

In recent discussions I have had with Novelx I understand they are
planning to introduce EDX by biasing the sample stage to increase the
landing voltage and thereby generate the X-rays needed for an elemental
map. We are considering a purchase of their benchtop SEM now for the high
spatial resolution it provides and upgrading it later to get the EDX
capability.

Chris Walker

wesaia-at-iastate.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I get the impression that the Novel-X mySEM is fundamentally different that 'normal' SEM's.
}
} It seems to be limited in working distance. Its maximum voltage is listed as 2 kV, so those factors pretty well rule out EDS.
}
} The maximum scan field is listed as 1x1 mm with a low magnification limit of 250x. I often bump up against our SEM's 25x low mag limit, so the mySEM would not be practical for me.
}
} Undoubtedly it will find some useful niches. Just make sure it is suited to your needs.
}
} Warren S.
}
}
} -----Original Message-----
} X-from: cw23-at-ohm.york.ac.uk [mailto:cw23-at-ohm.york.ac.uk]
} Sent: Friday, October 30, 2009 4:38 AM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Re: Benchtop SEMs
}
} Field emission sources provide better spatial resolution than
} traditional thermionic sources and
} I believe Novelx is the only company offering a benchtop Field Emission SEM.
}
} Chris Walker
} Department of Electronics
} University of York,
} Heslington,
} York,
} YO10 5DD.
}
} ZZhang-at-uwyo.edu wrote:
}
} } The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).
} }
} } The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.
} }
} } It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.
} }
} } It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.
} }
} } Hope this helps.
} }
} } Zhaojie
} }
} }
} } Zhaojie Zhang, Ph.D.
} } Director, Microscopy Core Facility
} } Department of Zoology and Physiology
} } University of Wyoming
} } Laramie, WY 82071
} } zzhang-at-uwyo.edu
} } 307-766-3038
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
} } Sent: Thursday, October 29, 2009 11:14 AM
} } To: Z.J. Zhang
} } Subject: [Microscopy] Re: viaWWW: Benchtop SEM, biggest bang for buck??
} }
} }
} } I also would like to know about the performance of these bench-top SEM
} } instruments and how they compare with full-size ones. And, to have some
} } orientation on its price. I know actual quotations would depend on many
} } different factors and can differ from lab to lab, but just to get an idea of
} } which level of funds has one to get to afford them.
} }
} } Many thanks,
} }
} } Antonio D. Molina-Garcia
} }
} } Instituto del Frío (CSIC)
} } José Antonio Nováis, 10
} } Ciudad Universitaria
} } 28040 Madrid
} } España
} }
} } Tel +34 915445607 Fax +34 915493627 E-mail : ifrm111-at-if.csic.es
} } http://www.if.csic.es/ingiind.htm
} }
} }
} } ----- Original Message -----
} } X-from: {jason.gillen-at-encorewire.com}
} } To: {ifrm111-at-if.csic.es}
} } Sent: Thursday, October 29, 2009 12:15 AM
} } Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??
} }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} } } ---------------------------------------------------------------------------
} } } Remember this posting is most likely not from a Subscriber, so when
} } } replying
} } } please copy both jason.gillen-at-encorewire.com as well as the
} } } MIcroscopy Listserver
} } } ---------------------------------------------------------------------------
} } }
} } } Email: jason.gillen-at-encorewire.com
} } } Name: Gillenium
} } }
} } } Organization: Industry
} } }
} } } Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??
} } }
} } } Question: New lab comming online and need experienced information on
} } } the "desktop" SEM units available now. I have read literature on
} } } FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
} } } also has a unit that offers X-ray analysis as well at a substantially
} } } higher price ca. $200K USD. I have never used one of these
} } } "benchtop" units and would appreciate any experience with them, who
} } } makes the best one for the price, limitations etc. etc. Thanks
} } }
} } }
}
}
} ==============================Original Headers==============================
} 11, 37 -- From wesaia-at-iastate.edu Fri Oct 30 10:14:35 2009
} 11, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
} 11, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UFEZ1j007746
} 11, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 10:14:35 -0500
} 11, 37 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
} 11, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n9UFEVc1022777;
} 11, 37 -- Fri, 30 Oct 2009 10:14:31 -0500
} 11, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp
} 11, 37 -- id 1b59_eca20ee2_c566_11de_8ca6_001372578af6;
} 11, 37 -- Fri, 30 Oct 2009 10:14:31 -0500
} 11, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
} 11, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n9UFEUu3008245;
} 11, 37 -- Fri, 30 Oct 2009 10:14:30 -0500
} 11, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 11, 37 -- Fri, 30 Oct 2009 10:14:30 -0500
} 11, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 11, 37 -- Content-class: urn:content-classes:message
} 11, 37 -- MIME-Version: 1.0
} 11, 37 -- Content-Type: text/plain;
} 11, 37 -- charset="iso-8859-1"
} 11, 37 -- Subject: RE: [Microscopy] Re: Benchtop SEMs
} 11, 37 -- Date: Fri, 30 Oct 2009 10:15:11 -0500
} 11, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D727044F27A8-at-maire.eng.iastate.edu}
} 11, 37 -- In-Reply-To: {200910300937.n9U9bwMM025605-at-ns.microscopy.com}
} 11, 37 -- X-MS-Has-Attach:
} 11, 37 -- X-MS-TNEF-Correlator:
} 11, 37 -- Thread-Topic: [Microscopy] Re: Benchtop SEMs
} 11, 37 -- Thread-Index: AcpZRKydkiHXN/i1QgKdAv4c9OQdEwALec+Q
} 11, 37 -- References: {200910300937.n9U9bwMM025605-at-ns.microscopy.com}
} 11, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 11, 37 -- To: {Microscopy-at-microscopy.com}
} 11, 37 -- Cc: {jason.gillen-at-encorewire.com}
} 11, 37 -- X-OriginalArrivalTime: 30 Oct 2009 15:14:30.0889 (UTC) FILETIME=[AE00F590:01CA5973]
} 11, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.10.30.150328
} 11, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_MONEY 0, __FRAUD_419_MONEY_VALUE 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0'
} 11, 37 -- Content-Transfer-Encoding: 8bit
} 11, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9UFEZ1j007746
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
4, 27 -- From cw23-at-ohm.york.ac.uk Fri Oct 30 11:39:14 2009
4, 27 -- Received: from mail.elec.york.ac.uk (mailgw0.elec.york.ac.uk [144.32.152.4])
4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UGdDCI027207
4, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Oct 2009 11:39:14 -0500
4, 27 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
4, 27 -- by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
4, 27 -- id 1N3uVK-00073y-L2
4, 27 -- for Microscopy-at-Microscopy.Com; Fri, 30 Oct 2009 16:39:10 +0000
4, 27 -- Message-ID: {4AEB16A4.2030202-at-ohm.york.ac.uk}
4, 27 -- Date: Fri, 30 Oct 2009 16:39:00 +0000
4, 27 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
4, 27 -- Organization: Department of Electronics, The University of York
4, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
4, 27 -- MIME-Version: 1.0
4, 27 -- To: Microscopy-at-Microscopy.Com
4, 27 -- Subject: Re: [Microscopy] Benchtop SEMs
4, 27 -- References: {200910301524.n9UFOexc022281-at-ns.microscopy.com}
4, 27 -- In-Reply-To: {200910301524.n9UFOexc022281-at-ns.microscopy.com}
4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 27 -- Content-Transfer-Encoding: 8bit
4, 27 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
4, 27 -- X-YorkElec-MailScanner-ID: 1N3uVK-00073y-L2
4, 27 -- X-YorkElec-MailScanner: Found to be clean
4, 27 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
4, 27 -- X-YorkElec-MailScanner-Watermark: 1257525552.94949-at-2EJH7iia64SHNAfhtap34w
4, 27 -- X-Spam-Status: No
4, 27 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
==============================End of - Headers==============================




From: johnf-at-geology.wisc.edu
Date: Fri, 30 Oct 2009 11:50:33 -0500
Subject: [Microscopy] EBSD listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For those who have EBSD as one of their tools:

FYI:

One item discussed at the MAS EBSD 2008 topical workshop here in
Madison was the usefulness of a listserver for providing a forum for
discussion of a variety of issues related to EBSD. Well, only 17
months late, is such a listserver.

I invite you to sign up to the new ebsd listserver just created,
hosted here at UW-Madison

either

log in to the web interface at https://lists.wisc.edu/read/?forum=ebsd
and select "all forums" in left menu, then scroll down to ebsd in the
list, then click 'subscribe' in the right column and enter your email
address and name

or

send blank email to
subscribe-ebsd-at-lists.wisc.edu

Please wait a day or two before posting anything on it. Once there
are several dozen people on it I will send out a message to that
effect. (And also information about EBSD 2010, which will be held in
Madison May 24-26, with registration opening soon.)

Pass the word to others you know who should be on it...

John
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Department of Geoscience fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

==============================Original Headers==============================
13, 24 -- From johnf-at-geology.wisc.edu Fri Oct 30 11:50:33 2009
13, 24 -- Received: from mail.geology.wisc.edu (mail.geology.wisc.edu [144.92.206.10])
13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UGoXBD009045
13, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 11:50:33 -0500
13, 24 -- Received: from localhost (mail.geology.wisc.edu [127.0.0.1])
13, 24 -- by localhost (Postfix) with ESMTP id ED9715C8212
13, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 11:50:32 -0500 (CDT)
13, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu
13, 24 -- Received: from mail.geology.wisc.edu ([127.0.0.1])
13, 24 -- by localhost (mail.geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024)
13, 24 -- with ESMTP id LA-RsTdmpCO1 for {microscopy-at-microscopy.com} ;
13, 24 -- Fri, 30 Oct 2009 11:50:32 -0500 (CDT)
13, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57])
13, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
13, 24 -- (No client certificate requested)
13, 24 -- by mail.geology.wisc.edu (Postfix) with ESMTP id C30495C8214
13, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 11:50:32 -0500 (CDT)
13, 24 -- Mime-Version: 1.0
13, 24 -- Message-Id: {p06230911c710c9c7c40c-at-[144.92.206.57]}
13, 24 -- Date: Fri, 30 Oct 2009 11:50:32 -0500
13, 24 -- To: {microscopy-at-microscopy.com}
13, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu}
13, 24 -- Subject: EBSD listserver
13, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jared-at-lv-em.com
Date: Fri, 30 Oct 2009 12:10:16 -0500
Subject: [Microscopy] Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Since this has been a frequent issue, I will take a brief moment to
comment. Here is a disclaimer - Delong is the company which develops
and markets the LVEM5.

Here a few points on the LVEM5 benchtop electron microscope;

- It is a benchtop design with no special power or cooling requirements.
- It is actually three microscopes in one: TEM  with optional SEM
and/or STEM and Electron Diffraction
- It has a high-brightness field emission Shottky emitter that
requires minimal maintenance
- It is capable of resolutions of around 2.5nm in all three imaging modes
- It has higher contrast: up to 10 times higher contrast than a
conventional TEM WITHOUT STAIN
- It is affordable: A fraction of the cost of conventional electron
microscopes (Starting at $135,000 USD)


Feel free to visit our website www.lv-em.com  to browse through some
of the images we have taken with the LVEM5. If you like, I can send
you the product brochure and official price card as a PDF by email.

Please do not hesitate to contact me for more information.


Have a good day,


----------------------------

Jared Lapkovsky
Sales and Applications Engineer

LVEM5 Team
Delong America Inc.

Montreal, Canada

514.904.1202 ext. 701
www.lv-em.com


Come meet us at:

MRS Fall Exhibit (Hynes Convention Center, Boston, MA) December 1 - 3, Booth 909


On Fri, Oct 30, 2009 at 5:46 AM, {cw23-at-ohm.york.ac.uk} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Field emission sources provide better spatial resolution than
} traditional thermionic sources and
} I believe Novelx is the only company offering a benchtop Field Emission SEM.
}
} Chris Walker
} Department of Electronics
} University of York,
} Heslington,
} York,
} YO10 5DD.
}
} ZZhang-at-uwyo.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).
} }
} } The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.
} }
} } It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.
} }
} } It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.
} }
} } Hope this helps.
} }
} } Zhaojie
} }
} }
} } Zhaojie Zhang, Ph.D.
} } Director, Microscopy Core Facility
} } Department of Zoology and Physiology
} } University of Wyoming
} } Laramie, WY 82071
} } zzhang-at-uwyo.edu
} } 307-766-3038
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
} } Sent: Thursday, October 29, 2009 11:14 AM
} } To: Z.J. Zhang
} } Subject: [Microscopy] Re: viaWWW: Benchtop SEM, biggest bang for buck??
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} }      I also would like to know about the performance of these bench-top SEM
} } instruments and how they compare with full-size ones. And, to have some
} } orientation on its price. I know actual quotations would depend on many
} } different factors and can differ from lab to lab, but just to get an idea of
} } which level of funds has one to get to afford them.
} }
} } Many thanks,
} }
} } Antonio D. Molina-Garcia
} }
} } Instituto del Frío (CSIC)
} } José Antonio Nováis, 10
} } Ciudad Universitaria
} } 28040 Madrid
} } España
} }
} } Tel  +34 915445607    Fax  +34 915493627    E-mail :   ifrm111-at-if.csic.es
} } http://www.if.csic.es/ingiind.htm
} }
} }
} } ----- Original Message -----
} } X-from: {jason.gillen-at-encorewire.com}
} } To: {ifrm111-at-if.csic.es}
} } Sent: Thursday, October 29, 2009 12:15 AM
} } Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??
} }
} }
} }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } } To  Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
} } } ---------------------------------------------------------------------------
} } } Remember this posting is most likely not from a Subscriber, so when
} } } replying
} } } please  copy  both jason.gillen-at-encorewire.com as well as   the
} } } MIcroscopy Listserver
} } } ---------------------------------------------------------------------------
} } }
} } } Email: jason.gillen-at-encorewire.com
} } } Name: Gillenium
} } }
} } } Organization: Industry
} } }
} } } Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??
} } }
} } } Question: New lab comming online and need experienced information on
} } } the "desktop" SEM units available now.  I have read literature on
} } } FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
} } } also has a unit that offers X-ray analysis as well at a substantially
} } } higher price ca. $200K USD.  I have never used one of these
} } } "benchtop" units and would appreciate any experience with them, who
} } } makes the best one for the price, limitations etc. etc.   Thanks
} } }
} } }
} }
} }
} } ==============================Original Headers==============================
} } 26, 29 -- From ZZhang-at-uwyo.edu Thu Oct 29 12:44:43 2009
} } 26, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
} } 26, 29 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9THih0Z027206
} } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:44:43 -0500
} } 26, 29 -- Received: from ponyexpress-ht2.uwyo.edu (ponyexpress-ht2.uwyo.edu [10.84.60.209])
} } 26, 29 --     by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n9THiZp0024405
} } 26, 29 --     (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
} } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 11:44:41 -0600 (MDT)
} } 26, 29 --     (envelope-from ZZhang-at-uwyo.edu)
} } 26, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht2
} } 26, 29 --  ([10.84.60.209]) with mapi; Thu, 29 Oct 2009 11:44:41 -0600
} } 26, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
} } 26, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } 26, 29 -- Date: Thu, 29 Oct 2009 11:44:45 -0600
} } 26, 29 -- Subject: RE: Benchtop SEM, biggest bang for buck??
} } 26, 29 -- Thread-Topic: Benchtop SEM, biggest bang for buck??
} } 26, 29 -- Thread-Index: AcpYuyw8wl6LJJ/ZShir1Dy+wAR+bgAARZMw
} } 26, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE95525A14-at-ponyexpress-mb2}
} } 26, 29 -- References: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } 26, 29 -- In-Reply-To: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } 26, 29 -- Accept-Language: en-US
} } 26, 29 -- Content-Language: en-US
} } 26, 29 -- X-MS-Has-Attach:
} } 26, 29 -- X-MS-TNEF-Correlator:
} } 26, 29 -- acceptlanguage: en-US
} } 26, 29 -- Content-Type: text/plain; charset="iso-8859-1"
} } 26, 29 -- MIME-Version: 1.0
} } 26, 29 -- Content-Transfer-Encoding: 8bit
} } 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9THih0Z027206
} } ==============================End of - Headers==============================
} }
}
}
} ==============================Original Headers==============================
} 4, 27 -- From cw23-at-ohm.york.ac.uk Fri Oct 30 04:37:09 2009
} 4, 27 -- Received: from mail.elec.york.ac.uk (mailgw0.elec.york.ac.uk [144.32.152.4])
} 4, 27 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9U9b84u024599
} 4, 27 --        for {Microscopy-at-Microscopy.Com} ; Fri, 30 Oct 2009 04:37:08 -0500
} 4, 27 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
} 4, 27 --        by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
} 4, 27 --        id 1N3nut-0005xF-BL
} 4, 27 --        for Microscopy-at-Microscopy.Com; Fri, 30 Oct 2009 09:37:07 +0000
} 4, 27 -- Message-ID: {4AEAB3B8.5050807-at-ohm.york.ac.uk}
} 4, 27 -- Date: Fri, 30 Oct 2009 09:36:56 +0000
} 4, 27 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
} 4, 27 -- Organization: Department of Electronics, The University of York
} 4, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
} 4, 27 -- MIME-Version: 1.0
} 4, 27 -- To: Microscopy-at-Microscopy.Com
} 4, 27 -- Subject: Re: Benchtop SEMs
} 4, 27 -- References: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} 4, 27 -- In-Reply-To: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 4, 27 -- Content-Transfer-Encoding: 8bit
} 4, 27 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
} 4, 27 -- X-YorkElec-MailScanner-ID: 1N3nut-0005xF-BL
} 4, 27 -- X-YorkElec-MailScanner: Found to be clean
} 4, 27 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
} 4, 27 -- X-YorkElec-MailScanner-Watermark: 1257500227.4277-at-qh3QwG3i4HoZQ9oz8IaMWg
} 4, 27 -- X-Spam-Status: No
} 4, 27 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
} ==============================End of - Headers==============================


==============================Original Headers==============================
21, 33 -- From jared-at-lv-em.com Fri Oct 30 12:10:15 2009
21, 33 -- Received: from washington.serverhostcenter.com (washington.serverhostcenter.com [80.251.16.2])
21, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UHAF40025161
21, 33 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 12:10:15 -0500
21, 33 -- Received: from mail-yx0-f189.google.com ([209.85.210.189])
21, 33 -- by washington.serverhostcenter.com with esmtpsa (TLSv1:RC4-MD5:128)
21, 33 -- (Exim 4.69)
21, 33 -- (envelope-from {jared-at-lv-em.com} )
21, 33 -- id 1N3uzM-0001yM-VL
21, 33 -- for microscopy-at-microscopy.com; Fri, 30 Oct 2009 12:10:13 -0500
21, 33 -- Received: by yxe27 with SMTP id 27so3043663yxe.10
21, 33 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 10:10:12 -0700 (PDT)
21, 33 -- MIME-Version: 1.0
21, 33 -- Received: by 10.101.138.19 with SMTP id q19mr1979304ann.99.1256922611852; Fri,
21, 33 -- 30 Oct 2009 10:10:11 -0700 (PDT)
21, 33 -- In-Reply-To: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
21, 33 -- References: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
21, 33 -- Date: Fri, 30 Oct 2009 13:10:11 -0400
21, 33 -- Message-ID: {3629bb860910301010y11f317f6wb25615fca5e5e9d8-at-mail.gmail.com}
21, 33 -- Subject: Re: [Microscopy] Re: Benchtop SEMs
21, 33 -- From: Jared Lapkovsky {jared-at-lv-em.com}
21, 33 -- To: cw23-at-ohm.york.ac.uk, microscopy-at-microscopy.com
21, 33 -- Content-Type: text/plain; charset=ISO-8859-1
21, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
21, 33 -- X-AntiAbuse: Primary Hostname - washington.serverhostcenter.com
21, 33 -- X-AntiAbuse: Original Domain - microscopy.com
21, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
21, 33 -- X-AntiAbuse: Sender Address Domain - lv-em.com
21, 33 -- X-Source:
21, 33 -- X-Source-Args:
21, 33 -- X-Source-Dir:
21, 33 -- Content-Transfer-Encoding: 8bit
21, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9UHAF40025161
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Fri, 30 Oct 2009 13:09:29 -0500
Subject: [Microscopy] Airfuge manual needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
anybody out there, who has by chance a user manual of a Beckman-Coulter
Airfuge as a PDF?
I am looking for one but did not find it in the WWW.

Thanks,
Stefan



--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
6, 20 -- From stefan.diller-at-t-online.de Fri Oct 30 13:09:29 2009
6, 20 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UI9SSZ010417
6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 13:09:29 -0500
6, 20 -- Received: from fwd08.aul.t-online.de
6, 20 -- by mailout01.t-online.de with smtp
6, 20 -- id 1N3vui-0005WU-02; Fri, 30 Oct 2009 19:09:28 +0100
6, 20 -- Received: from [192.168.2.101] (GiX-QBZeQhvC6VEIm10ufL2DmAkdA03V2X2VCOBRUUfByKTl94aGh7mRSgYKsIlZHh-at-[93.222.101.110]) by fwd08.aul.t-online.de
6, 20 -- with esmtp id 1N3vue-0Fxw480; Fri, 30 Oct 2009 19:09:24 +0100
6, 20 -- Message-ID: {4AEB2BD4.1080500-at-t-online.de}
6, 20 -- Date: Fri, 30 Oct 2009 19:09:24 +0100
6, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
6, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
6, 20 -- MIME-Version: 1.0
6, 20 -- To: microscopy-at-microscopy.com
6, 20 -- Subject: Airfuge manual needed
6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-ID: GiX-QBZeQhvC6VEIm10ufL2DmAkdA03V2X2VCOBRUUfByKTl94aGh7mRSgYKsIlZHh
6, 20 -- X-TOI-MSGID: e072ad4d-2429-48fb-99f1-808ce8d53bdd
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Fri, 30 Oct 2009 13:47:29 -0500
Subject: [Microscopy] Re: Airfuge manual needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes, Stefan, there are copies of the manual out there.

Please remind me next week. I will have to find it in the files and
make a .pdf of it. It will be the original manual, from my EM-90 work
in the 1980's.

Surprised that Beckman could not provide you with a manual, either hard
copy or .pdf.

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
7, 21 -- From paul_hazelton-at-umanitoba.ca Fri Oct 30 13:47:29 2009
7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UIlTDe026651
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 13:47:29 -0500
7, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
7, 21 -- (authenticated bits=0)
7, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n9UIlS9m004469;
7, 21 -- Fri, 30 Oct 2009 13:47:28 -0500 (CDT)
7, 21 -- Message-ID: {4AEB34C3.4050504-at-umanitoba.ca}
7, 21 -- Date: Fri, 30 Oct 2009 13:47:31 -0500
7, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
7, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
7, 21 -- MIME-Version: 1.0
7, 21 -- To: stefan.diller-at-t-online.de,
7, 21 -- Microscopy Listserver {microscopy-at-microscopy.com}
7, 21 -- Subject: Re: [Microscopy] Airfuge manual needed
7, 21 -- References: {200910301811.n9UIB0xE013155-at-ns.microscopy.com}
7, 21 -- In-Reply-To: {200910301811.n9UIB0xE013155-at-ns.microscopy.com}
7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 21 -- Content-Transfer-Encoding: 7bit
7, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: vladislav_speransky-at-nih.gov
Date: Fri, 30 Oct 2009 15:26:24 -0500
Subject: [Microscopy] Re: viaWWW: Stopping Times Allowed for Processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peggy,

Is there somebody who will be able to judge the quality of preservation?
If yes, I suggest you make that person part of the decision.
If no - then I don't understand what the worry is about. You just tell
them whether it is good or not, and give any reason you see fit.

More seriously, I believe 14 days vs 1 day in the fridge, undisturbed,
should not make much difference. And this is exactly the stage where I
would leave (and have left ;-)) them if too busy to process right
away. As possible ill effects of such delay I would think of osmotic
effects - swellings or shrinkage, or perhaps some extraction and
decrease in contrast, but so far I have not noticed that (no controls
checked, of course).

Have a great Halloween weekend!
Vlad

==============================Original Headers==============================
4, 23 -- From vladislav_speransky-at-nih.gov Fri Oct 30 15:26:24 2009
4, 23 -- Received: from out4.smtp.messagingengine.com (out4.smtp.messagingengine.com [66.111.4.28])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UKQOv9011907
4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 15:26:24 -0500
4, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41])
4, 23 -- by gateway1.messagingengine.com (Postfix) with ESMTP id E0CEFBC3FB;
4, 23 -- Fri, 30 Oct 2009 16:26:23 -0400 (EDT)
4, 23 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160])
4, 23 -- by compute1.internal (MEProxy); Fri, 30 Oct 2009 16:26:24 -0400
4, 23 -- DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed; d=messagingengine.com; h=message-id:from:to:content-type:content-transfer-encoding:subject:mime-version:date; s=smtpout; bh=7pczd2Ny+LKChQQF3o0dDk/oIxo=; b=chl82ju5dmq7G9aUa0bU/1hX3qJx9u3L/irHYK1Qv8xCB77xSXnQxJs9x9lqav8pz20kEXp9xIoYh12UjjKAewUP0ePQHDgoMtLis2wv4tYp1Sl+1cjjjeZKBMz4JTlK8Pq7ZaS4ns9t3YOToTP8jf5xz3MOA/8Zkk4LHo0o7nk=
4, 23 -- X-Sasl-enc: zSWqTYIcScM6bonAC/1HZR7dqCS9/DrXO+peIJ/HZoff 1256934383
4, 23 -- Received: from db459.niaid.nih.gov (db459.niaid.nih.gov [128.231.217.59])
4, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id 6C77949DD18;
4, 23 -- Fri, 30 Oct 2009 16:26:23 -0400 (EDT)
4, 23 -- Message-Id: {BC37181D-134D-44D1-81D3-FFB4401630E3-at-nih.gov}
4, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
4, 23 -- To: Microscopy-at-microscopy.com
4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- Subject: Re: [Microscopy] viaWWW: Stopping Times Allowed for Processing
4, 23 -- Mime-Version: 1.0 (Apple Message framework v936)
4, 23 -- Date: Fri, 30 Oct 2009 16:26:23 -0400
4, 23 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Mon, 2 Nov 2009 07:41:54 -0600
Subject: [Microscopy] Meeting Announcement: Midwest Microscopy and Microanalysis Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All,

The Midwest Microscopy and Microanalysis Society will hold its final meeting of 2009 on Friday, November 20th, at Baxter Corporate Headquarters in Deerfield, IL. Our corporate members are showing their appreciation for and support of M3S through a series of presentations on their newest instrumentation and techniques, and registration is free for M3S members and student members. The program and registration information can be found on our website under Meetings:

www.midwestmicroscopy.org

Jim Diorio has arranged an excellent program, and we look forward to seeing you there!

Regards,

Elaine Schumacher
M3S Program Coordinator


*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com}
Web Site: www.mccrone.com {http://www.mccrone.com/}



*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************



==============================Original Headers==============================
12, 24 -- From eschumacher-at-mccrone.com Mon Nov 2 07:41:53 2009
12, 24 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA2DfrX9010964
12, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Nov 2009 07:41:53 -0600
12, 24 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by
12, 24 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Mon, 2 Nov 2009 07:41:01
12, 24 -- -0600
12, 24 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
12, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
12, 24 -- Date: Mon, 2 Nov 2009 07:40:59 -0600
12, 24 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society
12, 24 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis
12, 24 -- Society
12, 24 -- Thread-Index: AcpbwhzN5eKk2Q5mSxGo6m3DrHzNuA==
12, 24 -- Message-ID: {874B1DB532886444A60A015EE363493F0B7734FE0D-at-mccronemsg07.tmg.mccrone.com}
12, 24 -- Accept-Language: en-US
12, 24 -- Content-Language: en-US
12, 24 -- X-MS-Has-Attach:
12, 24 -- X-MS-TNEF-Correlator:
12, 24 -- acceptlanguage: en-US
12, 24 -- Content-Type: text/plain; charset="us-ascii"
12, 24 -- MIME-Version: 1.0
12, 24 -- Content-Transfer-Encoding: 8bit
12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA2DfrX9010964
==============================End of - Headers==============================




From: l.tetley-at-bio.gla.ac.uk
Date: Mon, 2 Nov 2009 08:29:43 -0600
Subject: [Microscopy] viaWWW: 37th Scottish Microscopy Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both l.tetley-at-bio.gla.ac.uk as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: l.tetley-at-bio.gla.ac.uk
Name: Laurence Tetley

Organization: University of Glasgow, Scotland, UK

Title-Subject: [Filtered] Meeting Announcement

Question: 37th Scottish Microscopy Symposium


Wednesday 4th November 2009, Hunter Halls, University of Glasgow,
Glasgow G12 8QQ, Scotland, UK


Registration extended - with a 60% discount for students - arrvive
early and pay by cash/cheque


details via http://www.scottishmicroscopygroup.org.uk/


- main programme available at http://www.gla.ac.uk/ibls/II/em/SMG/programme.pdf


Trade Exhibition - 18 exhibitors confirmed.



Login Host: 130.209.6.42
---------------------------------------------------------------------------

==============================Original Headers==============================
18, 11 -- From zaluzec-at-microscopy.com Mon Nov 2 08:29:43 2009
18, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
18, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA2ETgWh027726
18, 11 -- for {microscopy-at-microscopy.com} ; Mon, 2 Nov 2009 08:29:42 -0600
18, 11 -- Mime-Version: 1.0
18, 11 -- Message-Id: {p06240801c7149d4455cb-at-[206.69.208.22]}
18, 11 -- Date: Mon, 2 Nov 2009 08:29:41 -0600
18, 11 -- To: microscopy-at-microscopy.com
18, 11 -- From: l.tetley-at-bio.gla.ac.uk (by way of MicroscopyListserver)
18, 11 -- Subject: viaWWW: 37th Scottish Microscopy Symposium
18, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Mon, 2 Nov 2009 09:05:44 -0600
Subject: [Microscopy] Re: Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have the Hitachi TM-1000. One thing that hasn't been mentioned is that
this instrument has a fixed accelerating voltage of 15 kV, so some
delicate structures have to be sputtered in order to show up well (e.g.
epicuticular waxes on plant leaves, etc.). Also, the software appears to
be targeted to novice users. It would be nice if there was a more
advanced version, with waveform traces, more convenient stigmation
controls, better auto-file naming, more magnification presets, etc.
However, it is nice that you just plug it in - no building
modifications, no water chiller, etc., needed - and you can be imaging.
Mine does show some vibration at higher magnification, so it might be
better on a small anti-vibration table. Also, because the instrument
only has a BSE detector, it is sensitive to areas of the specimen with
higher atomic number. This can make some specimens difficult to image,
like rice leaf with its large number of silica bodies, for example. The
silica bodies are so bright that it is difficult to see much else in
their vicinity. So even though you can view hydrated samples on the low
vacuum mode, most samples end up critical point dried and sputter coated
for one reason or another. I agree that it is good for some things, but
it isn't a total replacement for a full-size SEM.

Andy Bowling, Ph.D.
Imaging and Microscopy Scientist
Dow AgroSciences
Indianapolis, IN


==============================Original Headers==============================
3, 33 -- From AJBowling-at-dow.com Mon Nov 2 09:05:44 2009
3, 33 -- Received: from mail168.messagelabs.com (mail168.messagelabs.com [216.82.253.195])
3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA2F5eoV011275
3, 33 -- for {microscopy-at-microscopy.com} ; Mon, 2 Nov 2009 09:05:43 -0600
3, 33 -- X-VirusChecked: Checked
3, 33 -- X-Env-Sender: AJBowling-at-dow.com
3, 33 -- X-Msg-Ref: server-11.tower-168.messagelabs.com!1257174261!11432219!17
3, 33 -- X-StarScan-Version: 6.1.3; banners=-,-,-
3, 33 -- X-Originating-IP: [216.99.65.22]
3, 33 -- Received: (qmail 4477 invoked from network); 2 Nov 2009 15:05:39 -0000
3, 33 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22)
3, 33 -- by server-11.tower-168.messagelabs.com with RC4-SHA encrypted SMTP; 2 Nov 2009 15:05:39 -0000
3, 33 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.3959);
3, 33 -- Mon, 2 Nov 2009 10:05:38 -0500
3, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 33 -- Content-class: urn:content-classes:message
3, 33 -- MIME-Version: 1.0
3, 33 -- Content-Type: text/plain;
3, 33 -- charset="us-ascii"
3, 33 -- Subject: [Microscopy] Re: Benchtop SEMs
3, 33 -- Date: Mon, 2 Nov 2009 10:05:37 -0500
3, 33 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1013A83B4-at-USMDLMDOWX032.dow.com}
3, 33 -- In-Reply-To: {200910302113.n9ULD0Q4027501-at-ns.microscopy.com}
3, 33 -- X-MS-Has-Attach:
3, 33 -- X-MS-TNEF-Correlator:
3, 33 -- Thread-Topic: [Microscopy] Re: Benchtop SEMs
3, 33 -- Thread-Index: AcpZpcs2HEPwd98ZT5+awAjjqeNBEgCJ8gFg
3, 33 -- References: {200910302113.n9ULD0Q4027501-at-ns.microscopy.com}
3, 33 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
3, 33 -- To: {microscopy-at-microscopy.com}
3, 33 -- X-OriginalArrivalTime: 02 Nov 2009 15:05:38.0471 (UTC) FILETIME=[EFE5AB70:01CA5BCD]
3, 33 -- Content-Transfer-Encoding: 8bit
3, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA2F5eoV011275
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Mon, 2 Nov 2009 12:32:38 -0600
Subject: [Microscopy] Re: Airfuge manual needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
thank you very much; I just got a manual as PDF.
If anyone needs a copy, please email.

Best regards,
Stefan



Stefan Diller schrieb:
} Dear All,
} anybody out there, who has by chance a user manual of a Beckman-Coulter
} Airfuge as a PDF?
} I am looking for one but did not find it in the WWW.
}
} Thanks,
} Stefan
}
}
}

--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
7, 22 -- From stefan.diller-at-t-online.de Mon Nov 2 12:32:38 2009
7, 22 -- Received: from mailout03.t-online.de (mailout03.t-online.de [194.25.134.81])
7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA2IWc0Z002551
7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 2 Nov 2009 12:32:38 -0600
7, 22 -- Received: from fwd04.aul.t-online.de
7, 22 -- by mailout03.t-online.de with smtp
7, 22 -- id 1N51hk-0000BE-04; Mon, 02 Nov 2009 19:32:36 +0100
7, 22 -- Received: from [192.168.2.101] (VrqocqZCrhfudKbc48r-zptPv3QEWRPCSS3BI4e+llCNB8lhNx8g-y+QU+WxTqkg4T-at-[93.222.101.23]) by fwd04.aul.t-online.de
7, 22 -- with esmtp id 1N51hW-08MLBo0; Mon, 2 Nov 2009 19:32:22 +0100
7, 22 -- Message-ID: {4AEF25B5.7050604-at-t-online.de}
7, 22 -- Date: Mon, 02 Nov 2009 19:32:21 +0100
7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
7, 22 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
7, 22 -- MIME-Version: 1.0
7, 22 -- To: microscopy-at-microscopy.com
7, 22 -- Subject: Re: Airfuge manual needed
7, 22 -- References: {4AEB2BD4.1080500-at-t-online.de}
7, 22 -- In-Reply-To: {4AEB2BD4.1080500-at-t-online.de}
7, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
7, 22 -- Content-Transfer-Encoding: 7bit
7, 22 -- X-ID: VrqocqZCrhfudKbc48r-zptPv3QEWRPCSS3BI4e+llCNB8lhNx8g-y+QU+WxTqkg4T
7, 22 -- X-TOI-MSGID: bb1427bd-8a06-4319-a60c-e734332cb016
==============================End of - Headers==============================




From: martin.sabel-at-bam.de
Date: Tue, 3 Nov 2009 07:45:50 -0600
Subject: [Microscopy] SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I need some advice concerning the examination of living termites using an
ESEM (Philips XL30).

Has anyone experience in that field? What about the vacuum and the beam
energy?

I can´t imagine that the conditions in the specimen chamber are
health-promoting for termites but for the client it is important that the
termite survives the procedure.

We are able to work at a vacuum in the specimen chamber of up to about 20
Torr (about 2666 Pa). Does anyone know about the minimum pressure that
termites are able to cope with?

Thanks in advance,

Martin Sabel


BAM - Federal Institute for Material Research and Testing
Division IV.2 Environmental Material and Product Properties
Unter den Eichen 44-46
12203 Berlin

phone: +49 (0)30 6392 5961
email: martin.sabel-at-bam.de
www.bam.de








==============================Original Headers==============================
15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3DjnF5022795
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50 -0600
15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49 -0000
15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
15, 25 -- To: {Microscopy-at-microscopy.com}
15, 25 -- Subject: SEM - investigating living termites
15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain;
15, 25 -- charset="iso-8859-1"
15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
15, 25 -- Content-Language: de
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3DjnF5022795
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Tue, 3 Nov 2009 08:00:48 -0600
Subject: [Microscopy] SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One of our users looked at living grain mites at 5 degrees C and about 5 Torr last week. I guess the highest pressure you can get good image quality would be best for their survival. Someone in the 1960s looked at living ants at high vacuum - I saw the images in an ancient "National Geographic" magazine.

Does anyone know about differential survival times (of anything) after being viewed in an ESEM?

Dave

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: 03 November 2009 13:51
To: David Patton

Hello everyone,

I need some advice concerning the examination of living termites using an
ESEM (Philips XL30).

Has anyone experience in that field? What about the vacuum and the beam
energy?

I can´t imagine that the conditions in the specimen chamber are
health-promoting for termites but for the client it is important that the
termite survives the procedure.

We are able to work at a vacuum in the specimen chamber of up to about 20
Torr (about 2666 Pa). Does anyone know about the minimum pressure that
termites are able to cope with?

Thanks in advance,

Martin Sabel


BAM - Federal Institute for Material Research and Testing
Division IV.2 Environmental Material and Product Properties
Unter den Eichen 44-46
12203 Berlin

phone: +49 (0)30 6392 5961
email: martin.sabel-at-bam.de
www.bam.de








==============================Original Headers==============================
15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3DjnF5022795
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50 -0600
15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49 -0000
15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
15, 25 -- To: {Microscopy-at-microscopy.com}
15, 25 -- Subject: SEM - investigating living termites
15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain;
15, 25 -- charset="iso-8859-1"
15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
15, 25 -- Content-Language: de
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3DjnF5022795
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
28, 41 -- From David.Patton-at-uwe.ac.uk Tue Nov 3 08:00:48 2009
28, 41 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
28, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3E0lrV005479
28, 41 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:00:47 -0600
28, 41 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
28, 41 -- id 65fc_48a992f6_c881_11de_9631_00142223915c;
28, 41 -- Tue, 03 Nov 2009 14:00:45 +0000
28, 41 -- Received: from egen-hub01.campus.ads.uwe.ac.uk
28, 41 -- (egen-hub01.uwe.ac.uk [164.11.251.45])
28, 41 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
28, 41 -- 2005)) with ESMTP id {0KSJ00COHDLA1U-at-mta01.uwe.ac.uk} for
28, 41 -- Microscopy-at-microscopy.com; Tue, 03 Nov 2009 14:00:46 +0000 (GMT)
28, 41 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3])
28, 41 -- by egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi; Tue,
28, 41 -- 03 Nov 2009 14:00:46 +0000
28, 41 -- Date: Tue, 03 Nov 2009 13:59:18 +0000
28, 41 -- From: David Patton {David.Patton-at-uwe.ac.uk}
28, 41 -- Subject: RE: [Microscopy] SEM - investigating living termites
28, 41 -- In-reply-to: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- To: "'martin.sabel-at-bam.de'" {martin.sabel-at-bam.de}
28, 41 -- Message-id:
28, 41 -- {A169BAD2C2DC6D418270CDC03DF5CDF4255820E0F4-at-EGEN-MBX02.campus.ads.uwe.ac.uk}
28, 41 -- MIME-version: 1.0
28, 41 -- Content-type: text/plain;
28, 41 -- charset="utf-8"
28, 41 -- Content-language: en-US
28, 41 -- Accept-Language: en-US, en-GB
28, 41 -- Thread-topic: [Microscopy] SEM - investigating living termites
28, 41 -- Thread-index: AcpcjLsR1A7eXPNXQJK09BFzjDXULgAAE1Hg
28, 41 -- acceptlanguage: en-US, en-GB
28, 41 -- X-MS-Has-Attach:
28, 41 -- X-MS-TNEF-Correlator:
28, 41 -- References: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- X-NAIMIME-Disclaimer: 1
28, 41 -- X-NAIMIME-Modified: 1
28, 41 -- X-NAI-Spam-Level: **
28, 41 -- X-NAI-Spam-Score: 2
28, 41 -- X-NAI-Spam-Rules: 4 Rules triggered
28, 41 -- INVALID_MSGID=1, DATE_FUTURE=0.5, MFE_BAD_MSGID=0.5, RV3397=0
28, 41 -- Content-Transfer-Encoding: 8bit
28, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id nA3E0lrV005479
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Tue, 3 Nov 2009 08:01:08 -0600
Subject: [Microscopy] Re: SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Martin,
in 1994 there had been a GEO article featuring David Scharf imaging
living bugs in the SEM.
Maybe you try to get in contact via http://www.scharfphoto.com

Best wishes,
Stefan



martin.sabel-at-bam.de schrieb:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello everyone,
}
} I need some advice concerning the examination of living termites using an
} ESEM (Philips XL30).
}
} Has anyone experience in that field? What about the vacuum and the beam
} energy?
}
} I can´t imagine that the conditions in the specimen chamber are
} health-promoting for termites but for the client it is important that the
} termite survives the procedure.
}
} We are able to work at a vacuum in the specimen chamber of up to about 20
} Torr (about 2666 Pa). Does anyone know about the minimum pressure that
} termites are able to cope with?
}
} Thanks in advance,
}
} Martin Sabel
}
}
} BAM - Federal Institute for Material Research and Testing
} Division IV.2 Environmental Material and Product Properties
} Unter den Eichen 44-46
} 12203 Berlin
}
} phone: +49 (0)30 6392 5961
} email: martin.sabel-at-bam.de
} www.bam.de
}
}
}
}
}
}
}
}
} ==============================Original Headers==============================
} 15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
} 15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
} 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3DjnF5022795
} 15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50 -0600
} 15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49 -0000
} 15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
} 15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
} 15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49 -0000
} 15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
} 15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
} 15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
} 15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49 -0000
} 15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
} 15, 25 -- To: {Microscopy-at-microscopy.com}
} 15, 25 -- Subject: SEM - investigating living termites
} 15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
} 15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
} 15, 25 -- MIME-Version: 1.0
} 15, 25 -- Content-Type: text/plain;
} 15, 25 -- charset="iso-8859-1"
} 15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
} 15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
} 15, 25 -- Content-Language: de
} 15, 25 -- Content-Transfer-Encoding: 8bit
} 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3DjnF5022795
} ==============================End of - Headers==============================
}

--
-----------------------------------------------------
Stefan Diller - Wissenschaftliche Photographie
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------



==============================Original Headers==============================
9, 23 -- From stefan.diller-at-t-online.de Tue Nov 3 08:01:07 2009
9, 23 -- Received: from mailout11.t-online.de (mailout11.t-online.de [194.25.134.85])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA3E17qj006113
9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:01:07 -0600
9, 23 -- Received: from fwd09.aul.t-online.de
9, 23 -- by mailout11.t-online.de with smtp
9, 23 -- id 1N5JwX-00064y-00; Tue, 03 Nov 2009 15:01:05 +0100
9, 23 -- Received: from [192.168.2.101] (GiVbyiZlohyhLvazvC93c9PuadAP0zOeBQpL+xv8OxaUqBlZhNSp1xP344sZsH-QDM-at-[93.222.104.217]) by fwd09.aul.t-online.de
9, 23 -- with esmtp id 1N5JwK-10ineC0; Tue, 3 Nov 2009 15:00:52 +0100
9, 23 -- Message-ID: {4AF03793.8060106-at-t-online.de}
9, 23 -- Date: Tue, 03 Nov 2009 15:00:51 +0100
9, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de}
9, 23 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
9, 23 -- MIME-Version: 1.0
9, 23 -- To: microscopy-at-microscopy.com
9, 23 -- Subject: Re: [Microscopy] SEM - investigating living termites
9, 23 -- References: {200911031352.nA3DqFsr029732-at-ns.microscopy.com}
9, 23 -- In-Reply-To: {200911031352.nA3DqFsr029732-at-ns.microscopy.com}
9, 23 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
9, 23 -- X-ID: GiVbyiZlohyhLvazvC93c9PuadAP0zOeBQpL+xv8OxaUqBlZhNSp1xP344sZsH-QDM
9, 23 -- X-TOI-MSGID: 66f93413-0885-4024-9827-bab4dd84300d
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3E17qj006113
==============================End of - Headers==============================




From: avergason-at-tetracore.com
Date: Tue, 3 Nov 2009 08:22:27 -0600
Subject: [Microscopy] viaWWW: Philips Quad Serial Interface Board Installation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both avergason-at-tetracore.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: avergason-at-tetracore.com
Name: Amy Vergason

Organization: Tetracore, Inc

Title-Subject: [Filtered] Quad Serial Interface Board Installation Instructions

Question: Hi Everyone,

I am looking for the installation instructions for the Phillips
CM-10/CM-12/or CM-30 Quad Serial Interface Kit, PW 6440/00. It
includes an Intel board and another hardware piece with part number
9432 064 40001, and perhaps some cables.

Thank you!
Amy

Login Host: 66.3.246.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Tue Nov 3 08:22:27 2009
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA3EMQ1J002914
8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:22:26 -0600
8, 12 -- Mime-Version: 1.0
8, 12 -- Message-Id: {p06240800c715ecfc065b-at-[206.69.208.22]}
8, 12 -- Date: Tue, 3 Nov 2009 08:22:25 -0600
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: avergason-at-tetracore.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Philips Quad Serial Interface Board Installation
8, 12 -- Instructions
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ron.doole-at-materials.ox.ac.uk
Date: Tue, 3 Nov 2009 09:17:21 -0600
Subject: [Microscopy] SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I recall a demonstration at MSA where a vendor was imaging aphids on a stick. They had to reduce the pressure below a certain level to get them to hold still for the image. They then raised the pressure and the bugs started moving around again. They repeated this cycle several times. I don't know what the long term survivability was, but I would think it was pretty good.

This was also done in a VP-SEM, not an E-SEM, as I recall. It seemed the pressure was around 1 Torr when they slowed down and stopped.

I wonder, what will you be examining about them? I find that many bugs are too large to fit completely into the field of view.

Warren S.

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: Tuesday, November 03, 2009 7:47 AM
To: wesaia-at-iastate.edu

To slow down moving arthropods one can lower the temperature. This may be less damaging than dropping the pressure.

Dave

-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: 03 November 2009 15:08
To: David Patton

I recall a demonstration at MSA where a vendor was imaging aphids on a stick. They had to reduce the pressure below a certain level to get them to hold still for the image. They then raised the pressure and the bugs started moving around again. They repeated this cycle several times. I don't know what the long term survivability was, but I would think it was pretty good.

This was also done in a VP-SEM, not an E-SEM, as I recall. It seemed the pressure was around 1 Torr when they slowed down and stopped.

I wonder, what will you be examining about them? I find that many bugs are too large to fit completely into the field of view.

Warren S.

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: Tuesday, November 03, 2009 7:47 AM
To: wesaia-at-iastate.edu

David,

I remember seeing a chart in an article or book from Audrey Glauert probably in the early 70's giving electron dose rate versus biological effect, including death. This was related to the then current interest in TEM in-vivo cells. I've had a quick look without success but if I find something I'll let you know. Maybe other's memories are better than mine and can help.

Regards,
Ron

Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: David.Patton-at-uwe.ac.uk [David.Patton-at-uwe.ac.uk]
Sent: 03 November 2009 14:11
To: Ron Doole

One of our users looked at living grain mites at 5 degrees C and about 5 Torr last week. I guess the highest pressure you can get good image quality would be best for their survival. Someone in the 1960s looked at living ants at high vacuum - I saw the images in an ancient "National Geographic" magazine.

Does anyone know about differential survival times (of anything) after being viewed in an ESEM?

Dave

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: 03 November 2009 13:51
To: David Patton

Hello everyone,

I need some advice concerning the examination of living termites using an
ESEM (Philips XL30).

Has anyone experience in that field? What about the vacuum and the beam
energy?

I can´t imagine that the conditions in the specimen chamber are
health-promoting for termites but for the client it is important that the
termite survives the procedure.

We are able to work at a vacuum in the specimen chamber of up to about 20
Torr (about 2666 Pa). Does anyone know about the minimum pressure that
termites are able to cope with?

Thanks in advance,

Martin Sabel


BAM - Federal Institute for Material Research and Testing
Division IV.2 Environmental Material and Product Properties
Unter den Eichen 44-46
12203 Berlin

phone: +49 (0)30 6392 5961
email: martin.sabel-at-bam.de
www.bam.de








==============================Original Headers==============================
15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3DjnF5022795
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50 -0600
15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49 -0000
15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
15, 25 -- To: {Microscopy-at-microscopy.com}
15, 25 -- Subject: SEM - investigating living termites
15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain;
15, 25 -- charset="iso-8859-1"
15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
15, 25 -- Content-Language: de
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3DjnF5022795
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus software and none were found


==============================Original Headers==============================
28, 41 -- From David.Patton-at-uwe.ac.uk Tue Nov 3 08:00:48 2009
28, 41 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66])
28, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3E0lrV005479
28, 41 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:00:47 -0600
28, 41 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp
28, 41 -- id 65fc_48a992f6_c881_11de_9631_00142223915c;
28, 41 -- Tue, 03 Nov 2009 14:00:45 +0000
28, 41 -- Received: from egen-hub01.campus.ads.uwe.ac.uk
28, 41 -- (egen-hub01.uwe.ac.uk [164.11.251.45])
28, 41 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
28, 41 -- 2005)) with ESMTP id {0KSJ00COHDLA1U-at-mta01.uwe.ac.uk} for
28, 41 -- Microscopy-at-microscopy.com; Tue, 03 Nov 2009 14:00:46 +0000 (GMT)
28, 41 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3])
28, 41 -- by egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi; Tue,
28, 41 -- 03 Nov 2009 14:00:46 +0000
28, 41 -- Date: Tue, 03 Nov 2009 13:59:18 +0000
28, 41 -- From: David Patton {David.Patton-at-uwe.ac.uk}
28, 41 -- Subject: RE: [Microscopy] SEM - investigating living termites
28, 41 -- In-reply-to: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- To: "'martin.sabel-at-bam.de'" {martin.sabel-at-bam.de}
28, 41 -- Message-id:
28, 41 -- {A169BAD2C2DC6D418270CDC03DF5CDF4255820E0F4-at-EGEN-MBX02.campus.ads.uwe.ac.uk}
28, 41 -- MIME-version: 1.0
28, 41 -- Content-type: text/plain;
28, 41 -- charset="utf-8"
28, 41 -- Content-language: en-US
28, 41 -- Accept-Language: en-US, en-GB
28, 41 -- Thread-topic: [Microscopy] SEM - investigating living termites
28, 41 -- Thread-index: AcpcjLsR1A7eXPNXQJK09BFzjDXULgAAE1Hg
28, 41 -- acceptlanguage: en-US, en-GB
28, 41 -- X-MS-Has-Attach:
28, 41 -- X-MS-TNEF-Correlator:
28, 41 -- References: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- X-NAIMIME-Disclaimer: 1
28, 41 -- X-NAIMIME-Modified: 1
28, 41 -- X-NAI-Spam-Level: **
28, 41 -- X-NAI-Spam-Score: 2
28, 41 -- X-NAI-Spam-Rules: 4 Rules triggered
28, 41 -- INVALID_MSGID=1, DATE_FUTURE=0.5, MFE_BAD_MSGID=0.5, RV3397=0
28, 41 -- Content-Transfer-Encoding: 8bit
28, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id nA3E0lrV005479
==============================End of - Headers==============================

==============================Original Headers==============================
37, 30 -- From ron.doole-at-materials.ox.ac.uk Tue Nov 3 09:17:21 2009
37, 30 -- Received: from relay3.mail.ox.ac.uk (relay3.mail.ox.ac.uk [163.1.2.165])
37, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA3FHKGE012536
37, 30 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 09:17:21 -0600
37, 30 -- Received: from smtp1.nexus.ox.ac.uk ([163.1.154.135] helo=exht01.ad.oak.ox.ac.uk)
37, 30 -- by relay3.mail.ox.ac.uk with esmtp (Exim 4.69)
37, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
37, 30 -- id 1N5L8K-0005kZ-AO; Tue, 03 Nov 2009 15:17:20 +0000
37, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.1.5]) by
37, 30 -- exht01.ad.oak.ox.ac.uk ([163.1.154.52]) with mapi; Tue, 3 Nov 2009 15:17:19
37, 30 -- +0000
37, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
37, 30 -- To: "David.Patton-at-uwe.ac.uk" {David.Patton-at-uwe.ac.uk}
37, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
37, 30 -- Date: Tue, 3 Nov 2009 15:17:19 +0000
37, 30 -- Subject: RE: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Topic: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Index: Acpcj3ynJcY2oHypThybTof1RlWaZAACCkwF
37, 30 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF9264CD12DB9-at-EXMBX02.ad.oak.ox.ac.uk}
37, 30 -- References: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- In-Reply-To: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- Accept-Language: en-US, en-GB
37, 30 -- Content-Language: en-GB
37, 30 -- X-MS-Has-Attach:
37, 30 -- X-MS-TNEF-Correlator:
37, 30 -- acceptlanguage: en-US, en-GB
37, 30 -- Content-Type: text/plain; charset="iso-8859-1"
37, 30 -- MIME-Version: 1.0
37, 30 -- Content-Transfer-Encoding: 8bit
37, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA3FHKGE012536
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 3 Nov 2009 11:32:54 -0600
Subject: [Microscopy] BSL-2 question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everybody out there in virus land,

I am getting a room ready for BSL-2 level virus imaging. I can't get any information regarding how safe the cells are once the virus has been transfected (?) in to them.

I have a BSL-2 level bio-hood, I have a room with a door and I have a brand, spanking new deconvolution with FRET and an incubator for live cell imaging.

I need to know if the people in the room are safe from the virus once it's inside the cells. I can't get the air pressure changed in the room so I need to know what other precautions we might need to take.

Thanks to all who can give me any pertinent information.

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.





==============================Original Headers==============================
12, 21 -- From vapatpxs-at-yahoo.com Tue Nov 3 11:32:54 2009
12, 21 -- Received: from web46114.mail.sp1.yahoo.com (web46114.mail.sp1.yahoo.com [68.180.199.131])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3HWrI9005463
12, 21 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Nov 2009 11:32:54 -0600
12, 21 -- Received: (qmail 62009 invoked by uid 60001); 3 Nov 2009 17:32:53 -0000
12, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1257269573; bh=XyJrhk5Yg/9zC4N/oQ6ZfNTN1FgrReWkOkVWHBNBhU4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=guLgR8grPzwU0dyF9UaUN8H4osL1WpbE2h7OcCwQ6OgOv0XcacDcMUVFMf8VJvGAsHvbsJVYuK+Do5LF1HxBHyPoBt8hLmJr1WrtH/+cJnBGNHcefdC1ocQ42ciQuDxF0TXl5mJnYOreXmUDK74PoO6UgbtcZLiyz3FMXWBVu6E=
12, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 21 -- s=s1024; d=yahoo.com;
12, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 21 -- b=mIpYtG5/RuprnK/UUA3oXelFQEq0oNBCOJpmgfVqHdjOn4HfximkzrkZhZ2K1/M7CPIu3w+es70FjRuy5O7vXAkVbcufdfERTd8WU9+9CVUFkX8n0QUHN4Y/UzqoF0MSOS0agD0dwbrRuPb84S8UmYLNTMpYjg97YNI5g8rfLFE=;
12, 21 -- Message-ID: {136226.61942.qm-at-web46114.mail.sp1.yahoo.com}
12, 21 -- X-YMail-OSG: j29hYzwVM1m4GFQ1Gx8DI4kEmefRDQQfMzBPci7.pNrvLj4rgsOcsQs6l4lqVFpPRZzg5tOKviGXw.5I_d633elV0v5ZhKDFWl8Bx9O7cZFqXQO__06j3Dl8AEsOGMuACL9WkqGF1UGXEJ1QRmSCBfQx2is0yFzw3vMYsixAefFG8n21UcI2ajbNA9beRnhgxSNlT.Abjlvf
12, 21 -- Received: from [132.239.85.200] by web46114.mail.sp1.yahoo.com via HTTP; Tue, 03 Nov 2009 09:32:53 PST
12, 21 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
12, 21 -- Date: Tue, 3 Nov 2009 09:32:53 -0800 (PST)
12, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 21 -- Subject: BSL-2 question
12, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
12, 21 -- HistoNet {histonet-at-lists.utsouthwestern.edu}
12, 21 -- MIME-Version: 1.0
12, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Tue, 3 Nov 2009 12:14:55 -0600
Subject: [Microscopy] Re: BSL-2 question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paula

Unfortunately, you do not say what the virus is. However, the
information you give suggests you need to work at level 2. You also
talk about the virus being transfected, as opposed to infection. As a
result, it is not really clear what you are working with. The rules are
different if you are talking about plasmids, viral vectors which are not
replication competent, viral vectors which will replicate, virus, etc.


However, as a general rule of thumb, it is best to presume that
anything infectious remains so until it is inactivated. Being inside
the cell is no guarantee of protection. Once the cells are infected,
and replication in the cell has proceeded you may have production of
infectious progeny, even if you didn't start with infectious material to
start with. Therefore, the material should be kept in your level 2 area
until it is inactivated. Inactivation means fixation with
Glutaraldehyde. Waste material should be treated with either Glut, or
preferably with bleach before it leaves the area. Alternatively, of
course, you may have the material autoclaved. However, if you do that,
do not chemically inactivate the waste or cells. It is not good for the
people who are around, or, for that matter, the autoclave.


I will send you a copy of my lab safety manual for your information. It
will be in Word form so you can adapt it for your use. While it will
have the different things I make the staff sign off on, it does not have
the MSDS sheets for the individual viruses we have in the lab, or some
of the things which I simply reference to elsewhere for saving time and
paper.


I will also send a .pdf of the final form so you can see how it is put
together - things sometimes get screwed up when we open in different
computers with different printers. Also, it guarantees you can open it
if you can't open word. Note, the manual is for my level 2
gastroenteric virus lab, recently modified for a lab partner my Chair
gave me who is doing Borna Disease Virus work. It is not for my EM
lab. The rules are relevant to what you need.


Call if you need to ask any questions.

Paul

Also, you really should have the room at negative pressure, or it is not
a proper BSL-2 room.

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
15, 21 -- From paul_hazelton-at-umanitoba.ca Tue Nov 3 12:14:55 2009
15, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA3IEtpe022010
15, 21 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 12:14:55 -0600
15, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
15, 21 -- (authenticated bits=0)
15, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id nA3IEssh005895;
15, 21 -- Tue, 3 Nov 2009 12:14:54 -0600 (CST)
15, 21 -- Message-ID: {4AF07325.9070709-at-umanitoba.ca}
15, 21 -- Date: Tue, 03 Nov 2009 12:15:01 -0600
15, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
15, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
15, 21 -- MIME-Version: 1.0
15, 21 -- To: vapatpxs-at-yahoo.com
15, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
15, 21 -- Subject: Re: [Microscopy] BSL-2 question
15, 21 -- References: {200911031734.nA3HYwvo008237-at-ns.microscopy.com}
15, 21 -- In-Reply-To: {200911031734.nA3HYwvo008237-at-ns.microscopy.com}
15, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
15, 21 -- Content-Transfer-Encoding: 7bit
15, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: rajeevrajvihar-at-gmail.com
Date: Tue, 3 Nov 2009 22:06:10 -0600
Subject: [Microscopy] viaWWW: Why coating is not required for TEM of polymer samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rajeevrajvihar-at-gmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rajeevrajvihar-at-gmail.com
Name: Rajeev

Organization: Indian Space Research Organization

Title-Subject: [Filtered] Why coating is not required for TEM of
polymer samples

Question: I have a doubt regarding the sample preparation for TEM and
SEM. For SEM of polymers, since the latter is non-conductive, we are
giving a metallic coating to take care of the accumulated charges
during analysis. However, for TEM, we are microtoming thin polymer
sections (70-100 nm thickness) and place it over a copper grid, and
directly observing without giving any coating. The accelerating
voltage for SEM is of the order of 10-30 kV whereas that for TEM is
of the order of 200-300 kV. Why a coating is generally not required
for TEM of polymer samples even if we are using a higher accelerating
voltage? Is it due to the thinness of the specimen so that electrons
can pass through it? Or is there any other reason?

Login Host: 117.199.0.111
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Nov 3 22:06:09 2009
6, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4468Wu029172
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 22:06:09 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c716ae1c4adb-at-[206.69.208.22]}
6, 11 -- Date: Tue, 3 Nov 2009 22:06:06 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: rajeevrajvihar-at-gmail.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Why coating is not required for TEM of polymer samples
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: grahamlj2-at-mail.nih.gov
Date: Tue, 3 Nov 2009 22:06:54 -0600
Subject: [Microscopy] viaWWW: Help staining DNA ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both grahamlj2-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: grahamlj2-at-mail.nih.gov
Name: Lesley Graham

Organization: SAIC

Title-Subject: [Filtered] staining

Question: I am in need of some staining techniques to increase
contrast for DNA, peptides, sugar, and proteins.

Thanks in advance.

Login Host: 129.43.43.192
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Nov 3 22:06:53 2009
7, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA446pUO030162
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 22:06:52 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c716ae3a51dd-at-[206.69.208.22]}
7, 11 -- Date: Tue, 3 Nov 2009 22:06:50 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: grahamlj2-at-mail.nih.gov (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Help staining DNA ...
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: corvos-at-aol.com
Date: Tue, 3 Nov 2009 22:08:08 -0600
Subject: [Microscopy] viaWWW: Material for Archives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both corvos-at-aol.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: corvos-at-aol.com
Name: Walter Protheroe

Organization: E-MAC, Inc

Title-Subject: [Filtered] Material for Archives

Question: All,

I am looking for an archive (university or other) for some of the
materials I have aquired over the years. With this material I have a
set of slides that one of my customers left me. I would like to find
them a home. Their are some good geological images with explinations.

Please RSVP...

Walter Protheroe
corvos-at-aol.com

Login Host: 64.12.116.15
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Nov 3 22:08:07 2009
9, 11 -- Received: from [206.69.208.22] ([130.202.238.72])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4480sK001331
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 22:08:02 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240803c716ae81628c-at-[206.69.208.22]}
9, 11 -- Date: Tue, 3 Nov 2009 22:07:59 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: corvos-at-aol.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Material for Archives
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Wed, 4 Nov 2009 04:03:30 -0600
Subject: [Microscopy] SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pretty much of no help with imaging a live termite what-so-ever [other have
commented more usefully on that], but perhaps interesting none the less: I
am reminded of the WETSEM™ techniques by QuantomiX Ltd., Nes Ziona, Israel,
who I had thought could image living cells under the SEM using their special
SEM capsule [when presumably everything has to hold its breath rather] -
although their website suggests only specially fixed but still very 'wet'
tissue/cells or samples are generally used.

Their technique certainly works for hydrated 'wet' samples like tissues &
cells or gels & creams, i.e. the special capsule protects the wet samples
from the vacuum. Can’t obviously see how this would work with a walking
termite, other than it might survive within an inner space-capsule of this
sort for a while. From memory termites are rather large, compared to ants
[pretty sure even a baby termite wouldn’t squish into the small QuantomiX
capsule and they might have to defy gravity and walk upside-down on the
membrane]. QuantomiX say “no coating or embedding of the sample are
required, enabling electron microscopy imaging with easy sample preparation
comparable to light microscopy” - although they seem to use special wet
tissue fixative/staining/contrast techniques instead, that actually seem a
little more complicated than sputter coating.

Quantomix might well have thought deeply for a minute or two on the
possibilities of imaging small living things under the SEM, so perhaps try
emailing them?

Link:

http://www.quantomix.com/tecnology01.html

http://www.quantomix.com/tecnology02.html

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy




-----Original Message-----
X-from: ron.doole-at-materials.ox.ac.uk [mailto:ron.doole-at-materials.ox.ac.uk]
Sent: 03 November 2009 15:24
To: kjmorris-at-well.ox.ac.uk

David,

I remember seeing a chart in an article or book from Audrey Glauert probably
in the early 70's giving electron dose rate versus biological effect,
including death. This was related to the then current interest in TEM
in-vivo cells. I've had a quick look without success but if I find something
I'll let you know. Maybe other's memories are better than mine and can help.

Regards,
Ron

Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: David.Patton-at-uwe.ac.uk [David.Patton-at-uwe.ac.uk]
Sent: 03 November 2009 14:11
To: Ron Doole

One of our users looked at living grain mites at 5 degrees C and about 5
Torr last week. I guess the highest pressure you can get good image quality
would be best for their survival. Someone in the 1960s looked at living
ants at high vacuum - I saw the images in an ancient "National Geographic"
magazine.

Does anyone know about differential survival times (of anything) after being
viewed in an ESEM?

Dave

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: 03 November 2009 13:51
To: David Patton

Hello everyone,

I need some advice concerning the examination of living termites using an
ESEM (Philips XL30).

Has anyone experience in that field? What about the vacuum and the beam
energy?

I can´t imagine that the conditions in the specimen chamber are
health-promoting for termites but for the client it is important that the
termite survives the procedure.

We are able to work at a vacuum in the specimen chamber of up to about 20
Torr (about 2666 Pa). Does anyone know about the minimum pressure that
termites are able to cope with?

Thanks in advance,

Martin Sabel


BAM - Federal Institute for Material Research and Testing
Division IV.2 Environmental Material and Product Properties
Unter den Eichen 44-46
12203 Berlin

phone: +49 (0)30 6392 5961
email: martin.sabel-at-bam.de
www.bam.de








==============================Original Headers==============================
15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
nA3DjnF5022795
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50
-0600
15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49
-0000
15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49
-0000
15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49
-0000
15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
15, 25 -- To: {Microscopy-at-microscopy.com}
15, 25 -- Subject: SEM - investigating living termites
15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain;
15, 25 -- charset="iso-8859-1"
15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
15, 25 -- Content-Language: de
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nA3DjnF5022795
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus
software and none were found


==============================Original Headers==============================
28, 41 -- From David.Patton-at-uwe.ac.uk Tue Nov 3 08:00:48 2009
28, 41 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk
[164.11.132.66])
28, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
nA3E0lrV005479
28, 41 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:00:47
-0600
28, 41 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk
with smtp
28, 41 -- id 65fc_48a992f6_c881_11de_9631_00142223915c;
28, 41 -- Tue, 03 Nov 2009 14:00:45 +0000
28, 41 -- Received: from egen-hub01.campus.ads.uwe.ac.uk
28, 41 -- (egen-hub01.uwe.ac.uk [164.11.251.45])
28, 41 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07
(built Jun 24
28, 41 -- 2005)) with ESMTP id {0KSJ00COHDLA1U-at-mta01.uwe.ac.uk} for
28, 41 -- Microscopy-at-microscopy.com; Tue, 03 Nov 2009 14:00:46 +0000 (GMT)
28, 41 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk
([fe80::7dcf:a903:5a0:acc3])
28, 41 -- by egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi;
Tue,
28, 41 -- 03 Nov 2009 14:00:46 +0000
28, 41 -- Date: Tue, 03 Nov 2009 13:59:18 +0000
28, 41 -- From: David Patton {David.Patton-at-uwe.ac.uk}
28, 41 -- Subject: RE: [Microscopy] SEM - investigating living termites
28, 41 -- In-reply-to: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- To: "'martin.sabel-at-bam.de'" {martin.sabel-at-bam.de}
28, 41 -- Message-id:
28, 41 --
{A169BAD2C2DC6D418270CDC03DF5CDF4255820E0F4-at-EGEN-MBX02.campus.ads.uwe.ac.uk}
28, 41 -- MIME-version: 1.0
28, 41 -- Content-type: text/plain;
28, 41 -- charset="utf-8"
28, 41 -- Content-language: en-US
28, 41 -- Accept-Language: en-US, en-GB
28, 41 -- Thread-topic: [Microscopy] SEM - investigating living termites
28, 41 -- Thread-index: AcpcjLsR1A7eXPNXQJK09BFzjDXULgAAE1Hg
28, 41 -- acceptlanguage: en-US, en-GB
28, 41 -- X-MS-Has-Attach:
28, 41 -- X-MS-TNEF-Correlator:
28, 41 -- References: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- X-NAIMIME-Disclaimer: 1
28, 41 -- X-NAIMIME-Modified: 1
28, 41 -- X-NAI-Spam-Level: **
28, 41 -- X-NAI-Spam-Score: 2
28, 41 -- X-NAI-Spam-Rules: 4 Rules triggered
28, 41 -- INVALID_MSGID=1, DATE_FUTURE=0.5, MFE_BAD_MSGID=0.5,
RV3397=0
28, 41 -- Content-Transfer-Encoding: 8bit
28, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id
nA3E0lrV005479
==============================End of - Headers==============================

==============================Original Headers==============================
37, 30 -- From ron.doole-at-materials.ox.ac.uk Tue Nov 3 09:17:21 2009
37, 30 -- Received: from relay3.mail.ox.ac.uk (relay3.mail.ox.ac.uk
[163.1.2.165])
37, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nA3FHKGE012536
37, 30 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 09:17:21
-0600
37, 30 -- Received: from smtp1.nexus.ox.ac.uk ([163.1.154.135]
helo=exht01.ad.oak.ox.ac.uk)
37, 30 -- by relay3.mail.ox.ac.uk with esmtp (Exim 4.69)
37, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
37, 30 -- id 1N5L8K-0005kZ-AO; Tue, 03 Nov 2009 15:17:20 +0000
37, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.1.5]) by
37, 30 -- exht01.ad.oak.ox.ac.uk ([163.1.154.52]) with mapi; Tue, 3 Nov
2009 15:17:19
37, 30 -- +0000
37, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
37, 30 -- To: "David.Patton-at-uwe.ac.uk" {David.Patton-at-uwe.ac.uk}
37, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
37, 30 -- Date: Tue, 3 Nov 2009 15:17:19 +0000
37, 30 -- Subject: RE: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Topic: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Index: Acpcj3ynJcY2oHypThybTof1RlWaZAACCkwF
37, 30 -- Message-ID:
{FB563F1F5A8DDB49A34CB648BD23ACF9264CD12DB9-at-EXMBX02.ad.oak.ox.ac.uk}
37, 30 -- References: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- In-Reply-To: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- Accept-Language: en-US, en-GB
37, 30 -- Content-Language: en-GB
37, 30 -- X-MS-Has-Attach:
37, 30 -- X-MS-TNEF-Correlator:
37, 30 -- acceptlanguage: en-US, en-GB
37, 30 -- Content-Type: text/plain; charset="iso-8859-1"
37, 30 -- MIME-Version: 1.0
37, 30 -- Content-Transfer-Encoding: 8bit
37, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nA3FHKGE012536
==============================End of - Headers==============================



==============================Original Headers==============================
59, 23 -- From kjmorris-at-well.ox.ac.uk Wed Nov 4 04:03:29 2009
59, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
59, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4A3Sva025801
59, 23 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 04:03:29 -0600
59, 23 -- Received: from dhcp285.well.ox.ac.uk ([129.67.45.128] helo=CytoWhizz)
59, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
59, 23 -- id 1N5ci8-0006xd-3Z
59, 23 -- for microscopy-at-microscopy.com; Wed, 04 Nov 2009 10:03:28 +0000
59, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
59, 23 -- To: {microscopy-at-microscopy.com}
59, 23 -- References: {200911031523.nA3FNks2026503-at-ns.microscopy.com}
59, 23 -- In-Reply-To: {200911031523.nA3FNks2026503-at-ns.microscopy.com}
59, 23 -- Subject: RE: [Microscopy] SEM - investigating living termites
59, 23 -- Date: Wed, 4 Nov 2009 10:03:28 -0000
59, 23 -- Message-ID: {000801ca5d36$0e3f5850$2abe08f0$-at-ox.ac.uk}
59, 23 -- MIME-Version: 1.0
59, 23 -- Content-Type: text/plain;
59, 23 -- charset="iso-8859-1"
59, 23 -- X-Mailer: Microsoft Office Outlook 12.0
59, 23 -- Thread-Index: AcpcmaO4XWoaYsu0SfmCcZy61WCKMwADADnA
59, 23 -- Content-Language: en-gb
59, 23 -- Content-Transfer-Encoding: 8bit
59, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA4A3Sva025801
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Wed, 4 Nov 2009 07:29:42 -0600
Subject: [Microscopy] BSL-2 question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As Paul said if you work with a BSL-2 virus, consider it infectious at all times. One cannot be sure that the cells do not release virus particles in the medium. On the good side all cells in culture are protected in sterile dishes/plates, so a direct contamination is not possible, but you must always consider possible accidents.

As long as I know BSL-2 containment does not require depressurized rooms (it starts with BSL-3).
Working with security-wanting virus requires specific rooms and behavior, this is not subject to personal opinions.
I guess that your concerns come from the sharing of the material (microscope and accessories) for BSL-2 and non-BSL-2 applications.
Here is my opinion:
- Only trained persons should work in that project (makes sense but you never know).
- Do not mix persons involved with the BSL-2 project and those who are not in the same lab
- Optimally the microscope and accessories should stay in the same room as the one used to manipulate the cells (at least for the time needed to finish this project). If this is not possible (but this is really advisable), the cells should be transported in a closed container. Take en-route accidents seriously!!
- The pieces in contact with potentially-infected material should be systematically desinfected after each manipulation, as well as the pieces in contact
with the gloves (did I say you need to wear gloves? ;-))
- Any accident/spilling, even the slightest, should be taken seriously and managed accordingly (and the personal made aware of that).
The key here is to use aware and trained personal and take the routine disinfection seriously.

In brief:  Be careful not paranoid.

regards,

Stephane



----- Original Message ----
X-from: "vapatpxs-at-yahoo.com" {vapatpxs-at-yahoo.com}
To: nizets2-at-yahoo.com
Sent: Tue, November 3, 2009 6:38:12 PM

Hello Everybody out there in virus land,

I am getting a room ready for BSL-2 level virus imaging.  I can't get any information regarding how safe the cells are once the virus has been transfected (?) in to them.

I have a BSL-2 level bio-hood, I have a room with a door and I have a brand, spanking new deconvolution with FRET and an incubator for live cell imaging.

I need to know if the people in the room are safe from the virus once it's inside the cells.  I can't get the air pressure changed in the room so I need to know what other precautions we might need to take.

Thanks to all who can give me any pertinent information.

Paula  :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.



     

==============================Original Headers==============================
12, 21 -- From vapatpxs-at-yahoo.com Tue Nov  3 11:32:54 2009
12, 21 -- Received: from web46114.mail.sp1.yahoo.com (web46114.mail.sp1.yahoo.com [68.180.199.131])
12, 21 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA3HWrI9005463
12, 21 --     for {Microscopy-at-Microscopy.Com} ; Tue, 3 Nov 2009 11:32:54 -0600
12, 21 -- Received: (qmail 62009 invoked by uid 60001); 3 Nov 2009 17:32:53 -0000
12, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1257269573; bh=XyJrhk5Yg/9zC4N/oQ6ZfNTN1FgrReWkOkVWHBNBhU4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=guLgR8grPzwU0dyF9UaUN8H4osL1WpbE2h7OcCwQ6OgOv0XcacDcMUVFMf8VJvGAsHvbsJVYuK+Do5LF1HxBHyPoBt8hLmJr1WrtH/+cJnBGNHcefdC1ocQ42ciQuDxF0TXl5mJnYOreXmUDK74PoO6UgbtcZLiyz3FMXWBVu6E=
12, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
12, 21 --  s=s1024; d=yahoo.com;
12, 21 --  h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
12, 21 --  b=mIpYtG5/RuprnK/UUA3oXelFQEq0oNBCOJpmgfVqHdjOn4HfximkzrkZhZ2K1/M7CPIu3w+es70FjRuy5O7vXAkVbcufdfERTd8WU9+9CVUFkX8n0QUHN4Y/UzqoF0MSOS0agD0dwbrRuPb84S8UmYLNTMpYjg97YNI5g8rfLFE=;
12, 21 -- Message-ID: {136226.61942.qm-at-web46114.mail.sp1.yahoo.com}
12, 21 -- X-YMail-OSG: j29hYzwVM1m4GFQ1Gx8DI4kEmefRDQQfMzBPci7.pNrvLj4rgsOcsQs6l4lqVFpPRZzg5tOKviGXw.5I_d633elV0v5ZhKDFWl8Bx9O7cZFqXQO__06j3Dl8AEsOGMuACL9WkqGF1UGXEJ1QRmSCBfQx2is0yFzw3vMYsixAefFG8n21UcI2ajbNA9beRnhgxSNlT.Abjlvf
12, 21 -- Received: from [132.239.85.200] by web46114.mail.sp1.yahoo.com via HTTP; Tue, 03 Nov 2009 09:32:53 PST
12, 21 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
12, 21 -- Date: Tue, 3 Nov 2009 09:32:53 -0800 (PST)
12, 21 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
12, 21 -- Subject: BSL-2 question
12, 21 -- To: MSA BB {Microscopy-at-Microscopy.Com} ,
12, 21 --        HistoNet {histonet-at-lists.utsouthwestern.edu}
12, 21 -- MIME-Version: 1.0
12, 21 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================






==============================Original Headers==============================
29, 24 -- From nizets2-at-yahoo.com Wed Nov 4 07:29:42 2009
29, 24 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225])
29, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA4DTfXt018138
29, 24 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 07:29:41 -0600
29, 24 -- Received: (qmail 92882 invoked by uid 60001); 4 Nov 2009 13:29:41 -0000
29, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1257341381; bh=G8TLKQmbSptcjtz7fMSBfwBLGpPRAlb4/b+lUuHH8hY=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=EpABLwRUuh0ufBK1o0WKTjkJKjbkbBlVbISTXlx6lPxUvp32eIEsPgk7ZLvl4uEXj3XSzNlY0Yi/i+W7aCzPV1H4aBTstSFP4NvenYnhk23S0G4j4oIN5LHwLfkpZsAeMJAwPlH+WfZfv6+Vkl6le/MCs3j/jjZIIBVoef2ebiI=
29, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
29, 24 -- s=s1024; d=yahoo.com;
29, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
29, 24 -- b=STUjhzU4qXdEqRdg4kXlbWdHiGqBZ1h86NHaDOBislDn6mLeP0ofK6r/vvOboMtgPy9T3Fpj6xpCp/qJS7wlRwBj+HMZhXHFQQg9KyHDwQtd96Z8Lw7cJu5LBirkfWtGydpbe62ZM3BYWf5Ll+GwZkUcSqKCzJp8l4tvTIpsaJU=;
29, 24 -- Message-ID: {279904.92304.qm-at-web110802.mail.gq1.yahoo.com}
29, 24 -- X-YMail-OSG: 1x6rnRUVM1mQjYbZolvHnY6Bkhd7nKm5cXuqPtXfTy1hqs0J6Lo-
29, 24 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Wed, 04 Nov 2009 05:29:40 PST
29, 24 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
29, 24 -- References: {200911031738.nA3HcCB4011873-at-ns.microscopy.com}
29, 24 -- Date: Wed, 4 Nov 2009 05:29:40 -0800 (PST)
29, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
29, 24 -- Subject: Re: [Microscopy] BSL-2 question
29, 24 -- To: microscopy-at-microscopy.com
29, 24 -- In-Reply-To: {200911031738.nA3HcCB4011873-at-ns.microscopy.com}
29, 24 -- MIME-Version: 1.0
29, 24 -- Content-Type: text/plain; charset=iso-8859-1
29, 24 -- Content-Transfer-Encoding: 8bit
29, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA4DTfXt018138
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 4 Nov 2009 08:11:24 -0600
Subject: [Microscopy] SEM - investigating living termites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The QuantomiX capsules only permit imaging of samples that are on the membrane directly or within a couple microns of it, so I doubt that it would be useful for anything nearly as large as a termite. Bacteria, etc., work fine, but Big Bugs----probably not.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com


-----Original Message-----
X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk]
Sent: Wednesday, November 04, 2009 4:06 AM
To: Tindall, Randy D.

Pretty much of no help with imaging a live termite what-so-ever [other have
commented more usefully on that], but perhaps interesting none the less: I
am reminded of the WETSEM(tm) techniques by QuantomiX Ltd., Nes Ziona, Israel,
who I had thought could image living cells under the SEM using their special
SEM capsule [when presumably everything has to hold its breath rather] -
although their website suggests only specially fixed but still very 'wet'
tissue/cells or samples are generally used.

Their technique certainly works for hydrated 'wet' samples like tissues &
cells or gels & creams, i.e. the special capsule protects the wet samples
from the vacuum. Can't obviously see how this would work with a walking
termite, other than it might survive within an inner space-capsule of this
sort for a while. From memory termites are rather large, compared to ants
[pretty sure even a baby termite wouldn't squish into the small QuantomiX
capsule and they might have to defy gravity and walk upside-down on the
membrane]. QuantomiX say "no coating or embedding of the sample are
required, enabling electron microscopy imaging with easy sample preparation
comparable to light microscopy" - although they seem to use special wet
tissue fixative/staining/contrast techniques instead, that actually seem a
little more complicated than sputter coating.

Quantomix might well have thought deeply for a minute or two on the
possibilities of imaging small living things under the SEM, so perhaps try
emailing them?

Link:

http://www.quantomix.com/tecnology01.html

http://www.quantomix.com/tecnology02.html

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford OX3 7BN,
United Kingdom.

Telephone: +44 (0)1865 287568
Email: kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy




-----Original Message-----
X-from: ron.doole-at-materials.ox.ac.uk [mailto:ron.doole-at-materials.ox.ac.uk]
Sent: 03 November 2009 15:24
To: kjmorris-at-well.ox.ac.uk

David,

I remember seeing a chart in an article or book from Audrey Glauert probably
in the early 70's giving electron dose rate versus biological effect,
including death. This was related to the then current interest in TEM
in-vivo cells. I've had a quick look without success but if I find something
I'll let you know. Maybe other's memories are better than mine and can help.

Regards,
Ron

Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
________________________________________
X-from: David.Patton-at-uwe.ac.uk [David.Patton-at-uwe.ac.uk]
Sent: 03 November 2009 14:11
To: Ron Doole

One of our users looked at living grain mites at 5 degrees C and about 5
Torr last week. I guess the highest pressure you can get good image quality
would be best for their survival. Someone in the 1960s looked at living
ants at high vacuum - I saw the images in an ancient "National Geographic"
magazine.

Does anyone know about differential survival times (of anything) after being
viewed in an ESEM?

Dave

-----Original Message-----
X-from: martin.sabel-at-bam.de [mailto:martin.sabel-at-bam.de]
Sent: 03 November 2009 13:51
To: David Patton

Hello everyone,

I need some advice concerning the examination of living termites using an
ESEM (Philips XL30).

Has anyone experience in that field? What about the vacuum and the beam
energy?

I can´t imagine that the conditions in the specimen chamber are
health-promoting for termites but for the client it is important that the
termite survives the procedure.

We are able to work at a vacuum in the specimen chamber of up to about 20
Torr (about 2666 Pa). Does anyone know about the minimum pressure that
termites are able to cope with?

Thanks in advance,

Martin Sabel


BAM - Federal Institute for Material Research and Testing
Division IV.2 Environmental Material and Product Properties
Unter den Eichen 44-46
12203 Berlin

phone: +49 (0)30 6392 5961
email: martin.sabel-at-bam.de
www.bam.de








==============================Original Headers==============================
15, 25 -- From martin.sabel-at-bam.de Tue Nov 3 07:45:50 2009
15, 25 -- Received: from mailex1.bam.de (mailex1.bam.de [141.63.4.14])
15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
nA3DjnF5022795
15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 07:45:50
-0600
15, 25 -- Received: (qmail 8465 invoked from network); 3 Nov 2009 13:45:49
-0000
15, 25 -- Received: from mailsv1.bam.de (141.63.128.37)
15, 25 -- by mailex1.bam.de with SMTP; 3 Nov 2009 13:45:49 -0000
15, 25 -- Received: (qmail 5858 invoked from network); 3 Nov 2009 13:45:49
-0000
15, 25 -- Received: from unknown (HELO WS0296) ([141.63.108.64])
15, 25 -- (envelope-sender {martin.sabel-at-bam.de} )
15, 25 -- by mailsv1.bam.de (qmail-ldap-1.03) with SMTP
15, 25 -- for {Microscopy-at-microscopy.com} ; 3 Nov 2009 13:45:49
-0000
15, 25 -- From: "Martin Sabel" {martin.sabel-at-bam.de}
15, 25 -- To: {Microscopy-at-microscopy.com}
15, 25 -- Subject: SEM - investigating living termites
15, 25 -- Date: Tue, 3 Nov 2009 14:45:45 +0100
15, 25 -- Message-ID: {000301ca5c8b$f1735f00$d45a1d00$-at-sabel-at-bam.de}
15, 25 -- MIME-Version: 1.0
15, 25 -- Content-Type: text/plain;
15, 25 -- charset="iso-8859-1"
15, 25 -- X-Mailer: Microsoft Office Outlook 12.0
15, 25 -- Thread-Index: Acpci/DHTARlZpxfQtC4GqEw4JfzHw==
15, 25 -- Content-Language: de
15, 25 -- Content-Transfer-Encoding: 8bit
15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nA3DjnF5022795
==============================End of - Headers==============================


This incoming email to UWE has been independently scanned for viruses by
McAfee anti-virus software and none were detected


This email was independently scanned for viruses by McAfee anti-virus
software and none were found


==============================Original Headers==============================
28, 41 -- From David.Patton-at-uwe.ac.uk Tue Nov 3 08:00:48 2009
28, 41 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk
[164.11.132.66])
28, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
nA3E0lrV005479
28, 41 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 08:00:47
-0600
28, 41 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk
with smtp
28, 41 -- id 65fc_48a992f6_c881_11de_9631_00142223915c;
28, 41 -- Tue, 03 Nov 2009 14:00:45 +0000
28, 41 -- Received: from egen-hub01.campus.ads.uwe.ac.uk
28, 41 -- (egen-hub01.uwe.ac.uk [164.11.251.45])
28, 41 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07
(built Jun 24
28, 41 -- 2005)) with ESMTP id {0KSJ00COHDLA1U-at-mta01.uwe.ac.uk} for
28, 41 -- Microscopy-at-microscopy.com; Tue, 03 Nov 2009 14:00:46 +0000 (GMT)
28, 41 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk
([fe80::7dcf:a903:5a0:acc3])
28, 41 -- by egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi;
Tue,
28, 41 -- 03 Nov 2009 14:00:46 +0000
28, 41 -- Date: Tue, 03 Nov 2009 13:59:18 +0000
28, 41 -- From: David Patton {David.Patton-at-uwe.ac.uk}
28, 41 -- Subject: RE: [Microscopy] SEM - investigating living termites
28, 41 -- In-reply-to: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- To: "'martin.sabel-at-bam.de'" {martin.sabel-at-bam.de}
28, 41 -- Message-id:
28, 41 --
{A169BAD2C2DC6D418270CDC03DF5CDF4255820E0F4-at-EGEN-MBX02.campus.ads.uwe.ac.uk}
28, 41 -- MIME-version: 1.0
28, 41 -- Content-type: text/plain;
28, 41 -- charset="utf-8"
28, 41 -- Content-language: en-US
28, 41 -- Accept-Language: en-US, en-GB
28, 41 -- Thread-topic: [Microscopy] SEM - investigating living termites
28, 41 -- Thread-index: AcpcjLsR1A7eXPNXQJK09BFzjDXULgAAE1Hg
28, 41 -- acceptlanguage: en-US, en-GB
28, 41 -- X-MS-Has-Attach:
28, 41 -- X-MS-TNEF-Correlator:
28, 41 -- References: {200911031351.nA3DpA7q028459-at-ns.microscopy.com}
28, 41 -- X-NAIMIME-Disclaimer: 1
28, 41 -- X-NAIMIME-Modified: 1
28, 41 -- X-NAI-Spam-Level: **
28, 41 -- X-NAI-Spam-Score: 2
28, 41 -- X-NAI-Spam-Rules: 4 Rules triggered
28, 41 -- INVALID_MSGID=1, DATE_FUTURE=0.5, MFE_BAD_MSGID=0.5,
RV3397=0
28, 41 -- Content-Transfer-Encoding: 8bit
28, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id
nA3E0lrV005479
==============================End of - Headers==============================

==============================Original Headers==============================
37, 30 -- From ron.doole-at-materials.ox.ac.uk Tue Nov 3 09:17:21 2009
37, 30 -- Received: from relay3.mail.ox.ac.uk (relay3.mail.ox.ac.uk
[163.1.2.165])
37, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nA3FHKGE012536
37, 30 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2009 09:17:21
-0600
37, 30 -- Received: from smtp1.nexus.ox.ac.uk ([163.1.154.135]
helo=exht01.ad.oak.ox.ac.uk)
37, 30 -- by relay3.mail.ox.ac.uk with esmtp (Exim 4.69)
37, 30 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} )
37, 30 -- id 1N5L8K-0005kZ-AO; Tue, 03 Nov 2009 15:17:20 +0000
37, 30 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.1.5]) by
37, 30 -- exht01.ad.oak.ox.ac.uk ([163.1.154.52]) with mapi; Tue, 3 Nov
2009 15:17:19
37, 30 -- +0000
37, 30 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk}
37, 30 -- To: "David.Patton-at-uwe.ac.uk" {David.Patton-at-uwe.ac.uk}
37, 30 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
37, 30 -- Date: Tue, 3 Nov 2009 15:17:19 +0000
37, 30 -- Subject: RE: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Topic: [Microscopy] RE: SEM - investigating living termites
37, 30 -- Thread-Index: Acpcj3ynJcY2oHypThybTof1RlWaZAACCkwF
37, 30 -- Message-ID:
{FB563F1F5A8DDB49A34CB648BD23ACF9264CD12DB9-at-EXMBX02.ad.oak.ox.ac.uk}
37, 30 -- References: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- In-Reply-To: {200911031411.nA3EB5T9001874-at-ns.microscopy.com}
37, 30 -- Accept-Language: en-US, en-GB
37, 30 -- Content-Language: en-GB
37, 30 -- X-MS-Has-Attach:
37, 30 -- X-MS-TNEF-Correlator:
37, 30 -- acceptlanguage: en-US, en-GB
37, 30 -- Content-Type: text/plain; charset="iso-8859-1"
37, 30 -- MIME-Version: 1.0
37, 30 -- Content-Transfer-Encoding: 8bit
37, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nA3FHKGE012536
==============================End of - Headers==============================



==============================Original Headers==============================
59, 23 -- From kjmorris-at-well.ox.ac.uk Wed Nov 4 04:03:29 2009
59, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
59, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4A3Sva025801
59, 23 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 04:03:29 -0600
59, 23 -- Received: from dhcp285.well.ox.ac.uk ([129.67.45.128] helo=CytoWhizz)
59, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
59, 23 -- id 1N5ci8-0006xd-3Z
59, 23 -- for microscopy-at-microscopy.com; Wed, 04 Nov 2009 10:03:28 +0000
59, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
59, 23 -- To: {microscopy-at-microscopy.com}
59, 23 -- References: {200911031523.nA3FNks2026503-at-ns.microscopy.com}
59, 23 -- In-Reply-To: {200911031523.nA3FNks2026503-at-ns.microscopy.com}
59, 23 -- Subject: RE: [Microscopy] SEM - investigating living termites
59, 23 -- Date: Wed, 4 Nov 2009 10:03:28 -0000
59, 23 -- Message-ID: {000801ca5d36$0e3f5850$2abe08f0$-at-ox.ac.uk}
59, 23 -- MIME-Version: 1.0
59, 23 -- Content-Type: text/plain;
59, 23 -- charset="iso-8859-1"
59, 23 -- X-Mailer: Microsoft Office Outlook 12.0
59, 23 -- Thread-Index: AcpcmaO4XWoaYsu0SfmCcZy61WCKMwADADnA
59, 23 -- Content-Language: en-gb
59, 23 -- Content-Transfer-Encoding: 8bit
59, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA4A3Sva025801
==============================End of - Headers==============================


==============================Original Headers==============================
71, 32 -- From TindallR-at-missouri.edu Wed Nov 4 08:11:23 2009
71, 32 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
71, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4EBNsW002755
71, 32 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 08:11:23 -0600
71, 32 -- X-IronPort-Anti-Spam-Filtered: true
71, 32 -- X-IronPort-Anti-Spam-Result: AgkFANAZ8UrRauUp/2dsb2JhbACcRrJCAQmGIIhMAoJSgWkEgWU
71, 32 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
71, 32 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 04 Nov 2009 08:11:21 -0600
71, 32 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
71, 32 -- Wed, 4 Nov 2009 08:11:21 -0600
71, 32 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
71, 32 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Wed, 4 Nov 2009
71, 32 -- 08:11:21 -0600
71, 32 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
71, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
71, 32 -- Date: Wed, 4 Nov 2009 08:11:19 -0600
71, 32 -- Subject: RE: [Microscopy] RE: SEM - investigating living termites
71, 32 -- Thread-Topic: [Microscopy] RE: SEM - investigating living termites
71, 32 -- Thread-Index: AcpdNmCM73PECydSQD2LZgc0q7ITmgAId8Fg
71, 32 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129B0E86F5-at-UM-EMAIL06.um.umsystem.edu}
71, 32 -- References: {200911041005.nA4A5gDb027565-at-ns.microscopy.com}
71, 32 -- In-Reply-To: {200911041005.nA4A5gDb027565-at-ns.microscopy.com}
71, 32 -- Accept-Language: en-US
71, 32 -- Content-Language: en-US
71, 32 -- X-MS-Has-Attach:
71, 32 -- X-MS-TNEF-Correlator:
71, 32 -- acceptlanguage: en-US
71, 32 -- Content-Type: text/plain; charset="iso-8859-1"
71, 32 -- MIME-Version: 1.0
71, 32 -- X-OriginalArrivalTime: 04 Nov 2009 14:11:21.0718 (UTC) FILETIME=[AF8C1960:01CA5D58]
71, 32 -- Content-Transfer-Encoding: 8bit
71, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA4EBNsW002755
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Wed, 4 Nov 2009 13:00:27 -0600
Subject: [Microscopy] RE: Why coating is not required for TEM of polymer specimens.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Rajeev,

Excellent question. Ultrathin (TEM) specimens are less prone to
charging than bulk samples for several reasons: 1. less mass (as you
already observed) to accommodate the static charge, 2. proximity to a
metal (usually copper) grid that dissipates charges to ground.
Nonetheless, TEM specimens do build up static charges, resulting in
specimen drift or even ejection of smaller specimen parts, usually
onto your objective aperture.

If the specimen is in close proximity to a grid bar (or lying over a
bar), usually there is little problem with static charging. However,
with non-conducting, particulate specimens that are far away from a
grid bar (and isolated), you will see major drifting, jumping and
disappearance of the specimen due to charging. In that case, a light
coating of carbon (by thermal evaporation) will usually solve the
problem. Carbon substrates are less likely to show charging but they
are fragile. Most people use Formvar/Carbon substrates, primarily for
stability. Just make sure the specimen is on the carbonized side and
that the carbon overlays the grid.

I got a charge out of your question......

John Bozzola


--
John J. Bozzola, Ph.D., Director
Integrated Microscopy and Graphics Experts
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901

==============================Original Headers==============================
7, 15 -- From bozzola-at-siu.edu Wed Nov 4 13:00:27 2009
7, 15 -- Received: from mail-px0-f188.google.com (mail-px0-f188.google.com [209.85.216.188])
7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4J0QWQ005474
7, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 13:00:27 -0600
7, 15 -- Received: by pxi26 with SMTP id 26so5039796pxi.22
7, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Nov 2009 11:00:26 -0800 (PST)
7, 15 -- MIME-Version: 1.0
7, 15 -- Received: by 10.114.19.30 with SMTP id 30mr2707941was.134.1257361226520; Wed,
7, 15 -- 04 Nov 2009 11:00:26 -0800 (PST)
7, 15 -- Date: Wed, 4 Nov 2009 13:00:26 -0600
7, 15 -- Message-ID: {ebc2299e0911041100y46abc879gbaa309d2c7803434-at-mail.gmail.com}
7, 15 -- Subject: RE: Why coating is not required for TEM of polymer specimens.
7, 15 -- From: John Bozzola {bozzola-at-siu.edu}
7, 15 -- To: MSAListserver {Microscopy-at-microscopy.com}
7, 15 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: Frank_Karl-at-lincolnelectric.com
Date: Wed, 4 Nov 2009 14:23:39 -0600
Subject: [Microscopy] Why coating is not required for TEM of polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Okay, I can't resist...

Two atoms are drifting through a matrix when suddenly one starts patting
himself down.

"What wrong? says the second atom.

"I just lost an electron," said the first.

"Are you sure?" the second one replies.

"Yes... (wait for it...) I'm positive.




--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
11, 23 -- From frank_karl-at-lincolnelectric.com Wed Nov 4 14:23:39 2009
11, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115])
11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA4KNdav023072
11, 23 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 14:23:39 -0600
11, 23 -- In-Reply-To: {200911041908.nA4J8c3O017351-at-ns.microscopy.com}
11, 23 -- Subject: Re: [Microscopy] RE: Why coating is not required for TEM of polymer
11, 23 -- specimens.
11, 23 -- To: Microscopy-at-microscopy.com
11, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
11, 23 -- Message-ID: {OF780AA106.033D78E4-ON85257664.006F5B07-85257664.006FF8F3-at-lincolnelectric.com}
11, 23 -- Date: Wed, 4 Nov 2009 15:23:12 -0500
11, 23 -- From: Frank_Karl-at-lincolnelectric.com
11, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 11/04/2009 03:23:13 PM,
11, 23 -- CD-MIME complete at 11/04/2009 03:23:13 PM,
11, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 11/04/2009 03:23:13 PM,
11, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
11, 23 -- 07, 2008) at 11/04/2009 03:23:13 PM,
11, 23 -- Serialize complete at 11/04/2009 03:23:13 PM
11, 23 -- MIME-Version: 1.0
11, 23 -- Content-Type: text/plain;
11, 23 -- charset="US-ASCII"
==============================End of - Headers==============================




From: graham-at-tll.org.sg
Date: Wed, 4 Nov 2009 21:35:15 -0600
Subject: [Microscopy] viaWWW: Job opportunity - Microscopy Technologist, Singapore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both graham-at-tll.org.sg as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: graham-at-tll.org.sg
Name: Graham Wright

Organization: Temasek Life Sciences Laboratory

Title-Subject: [Filtered] Job opportunity - Microscopy Technologist, Singapore

Question: An immediate vacancy for Microscopy Technologist is
available in Electron/Confocal Microscopy Unit at Temasek Life
Sciences Laboratory, Singapore (For information about TLL:
http://www.tll.org.sg For information about the microscopy facility:
http://microscopy.tll.org.sg/index.htm). Please forward this email on
to anyone that may be interested.

Responsibilities:

* General maintenance of microscopes and accessories
* Training and supporting users in the basics of microscopy,
advanced applications and image analysis software
* Liaising with manufacturers for repair and upgrade of microscopes

Requirements:

* Min Diploma (Biological Sciences or related fields) (Fresh
graduates are welcome to apply)
* Good computer skills
* Good English and Chinese communication skills for purpose of
communicating with Chinese counterparts
* Experience in light microscopy with experience in
confocal/electron microscope preferred

The salary given is commensurable to educational qualifications and
working experience of the candidates. Benefits include annual leave,
medical and dental benefits, etc.

Interested individuals should email their curriculum vitae to:

Human Resource
Temasek Life Sciences Laboratory Limited
1 Research Link National University of Singapore Singapore 117604
E hr-at-tll.org.sg

For more information, pls email graham-at-tll.org.sg

Closing date: 13 Nov 2009

(Please note that only shortlisted candidates will be notified)

Thanks,
Graham

-----
Graham Wright
Microscopy & Imaging Facility

Temasek Life Sciences Laboratory
1 Research Link
National University of Singapore
Singapore 117604

P: +65 6872 8406
E: graham-at-tll.org.sg
W: http://microscopy.tll.org.sg

Login Host: 137.132.243.142
---------------------------------------------------------------------------

==============================Original Headers==============================
20, 11 -- From zaluzec-at-microscopy.com Wed Nov 4 21:35:15 2009
20, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
20, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA53ZDMA029453
20, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2009 21:35:15 -0600
20, 11 -- Mime-Version: 1.0
20, 11 -- Message-Id: {p06240800c717f85eb3cf-at-[206.69.208.22]}
20, 11 -- Date: Wed, 4 Nov 2009 21:35:13 -0600
20, 11 -- To: microscopy-at-microscopy.com
20, 11 -- From: graham-at-tll.org.sg (by way of MicroscopyListserver)
20, 11 -- Subject: viaWWW: Job opportunity - Microscopy Technologist, Singapore
20, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bharris-at-uoguelph.ca
Date: Thu, 5 Nov 2009 10:02:38 -0600
Subject: [Microscopy] TEM: Preserving tissue for embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Day: We are trying to preserve tobacco leaves infected by virus. To date we have tried room temp methods using vacuum during an aldehyde cocktail fixation and then moved on to high press freezing followed by freeze substitution.
To date we have not been able to preserve a cell full of cytoplasm and organelles. If anyone is having success in either of these techniques and could enlighten us we would be very grateful. bob harris

--
Electron Microscopy Unit
New Science Complex
488 Gordon St
University of Guelph
Guelph, Ontario, Canada, N1G 2W1
Phone: 519-824-4120 ext. 56409
Fax : 519-837-1802

==============================Original Headers==============================
2, 23 -- From bharris-at-uoguelph.ca Thu Nov 5 10:02:38 2009
2, 23 -- Received: from esa-annu.mail.uoguelph.ca (esa-annu.mail.uoguelph.ca [131.104.91.36])
2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA5G2crQ019534
2, 23 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 10:02:38 -0600
2, 23 -- X-IronPort-Anti-Spam-Filtered: true
2, 23 -- X-IronPort-Anti-Spam-Result: ApwEAFmF8kqDaFvM/2dsb2JhbACBToMkymmCFQmOOgKBMIEigRZTBA
2, 23 -- X-IronPort-AV: E=Sophos;i="4.44,687,1249272000";
2, 23 -- d="scan'208";a="52631607"
2, 23 -- Received: from huron.cs.uoguelph.ca (HELO zcs1.mail.uoguelph.ca) ([131.104.91.204])
2, 23 -- by esa-annu-pri.mail.uoguelph.ca with ESMTP; 05 Nov 2009 11:02:36 -0500
2, 23 -- Received: from zcs1.mail.uoguelph.ca (localhost.localdomain [127.0.0.1])
2, 23 -- by zcs1.mail.uoguelph.ca (Postfix) with ESMTP id EDBF510D31B
2, 23 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 11:02:35 -0500 (EST)
2, 23 -- Date: Thu, 5 Nov 2009 11:02:35 -0500 (EST)
2, 23 -- From: Robert J Harris {bharris-at-uoguelph.ca}
2, 23 -- To: "microscopy-at-microscopy.com microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 23 -- Message-ID: {1308435821.18622501257436955929.JavaMail.root-at-huron.cs.uoguelph.ca}
2, 23 -- Subject: TEM: Preserving tissue for embedding
2, 23 -- MIME-Version: 1.0
2, 23 -- Content-Type: text/plain; charset=utf-8
2, 23 -- Content-Transfer-Encoding: 7bit
2, 23 -- X-Originating-IP: [131.104.190.70]
2, 23 -- X-Mailer: Zimbra 5.0.11_GA_2695.RHEL4_64 (ZimbraWebClient - IE7 (Win)/5.0.11_GA_2695.RHEL4_64)
==============================End of - Headers==============================




From: maloneyb-at-fiu.edu
Date: Thu, 5 Nov 2009 10:58:37 -0600
Subject: [Microscopy] Camera lucida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group: by chance does anyone have a used camera lucida for sale?
Thanks
Barbara

==============================Original Headers==============================
1, 16 -- From maloneyb-at-fiu.edu Thu Nov 5 10:58:37 2009
1, 16 -- Received: from fiumailsmtp.fiu.edu (fiuht02.ad.fiu.edu [131.94.75.140])
1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA5GwaVo004240
1, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 10:58:37 -0600
1, 16 -- Received: from [131.94.220.251] (131.94.220.251) by fiumailsmtp.fiu.edu
1, 16 -- (192.168.251.11) with Microsoft SMTP Server (TLS) id 8.1.393.1; Thu, 5 Nov
1, 16 -- 2009 11:58:35 -0500
1, 16 -- Message-ID: {4AF3043B.60308-at-fiu.edu}
1, 16 -- Date: Thu, 5 Nov 2009 11:58:35 -0500
1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu}
1, 16 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
1, 16 -- MIME-Version: 1.0
1, 16 -- To: Microscopy-at-microscopy.com
1, 16 -- Subject: Camera lucida
1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
1, 16 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: fadamic-at-gmail.com
Date: Thu, 5 Nov 2009 18:13:38 -0600
Subject: [Microscopy] viaWWW: used microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both fadamic-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: fadamic-at-gmail.com
Name: Fabio D'Amico

Organization: University of catania, Italy

Title-Subject: [Filtered] microtome

Question: I would be interested in obtaining a used microtome for
histology and I was wondering if you have one in surplus in your
institute that could be donated.

Thanks

Fabio

Login Host: 151.97.169.107
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Nov 5 18:13:38 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA60DbRH004578
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 18:13:37 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240805c7191a92b927-at-[206.69.208.22]}
8, 11 -- Date: Thu, 5 Nov 2009 18:13:35 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: fadamic-at-gmail.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: used microtome
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lesley.bechtold-at-jax.org
Date: Thu, 5 Nov 2009 18:14:18 -0600
Subject: [Microscopy] viaWWW: Image Analysis of Proteins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lesley.bechtold-at-jax.org as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lesley.bechtold-at-jax.org
Name: Lesley Bechtold

Organization: The Jackson Laboratory

Title-Subject: [Filtered] Image Analysis of Proteins

Question: I am going to be preparing some proteins spreads for TEM
for one the researchers here, much like you do for DNA spreads on
coated grids followed by rotary shadowing. We plan on capturing many
images. He would like to do some image analysis on these images in
order to demonstrate that there is a (statistical) difference between
the wild type and recombinant forms. We want to know if there are
any image analysis software packages out there that folks are already
using for this kind of analysis.

Thank you in advance!

Lesley Bechtold

Login Host: 209.222.206.50
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Thu Nov 5 18:14:18 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA60EHxW005320
8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 18:14:18 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240806c7191ac9c621-at-[206.69.208.22]}
8, 11 -- Date: Thu, 5 Nov 2009 18:14:16 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: lesley.bechtold-at-jax.org (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Image Analysis of Proteins
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jawolff-at-mail.wsu.edu
Date: Thu, 5 Nov 2009 18:14:52 -0600
Subject: [Microscopy] viaWWW: Job opening: electron microprobe operator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jawolff-at-mail.wsu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jawolff-at-mail.wsu.edu
Name: John Wolff

Organization: Washington State University

Title-Subject: [Filtered] Job opening: electron microprobe operator

Question: Electron Microprobe and X-ray
Diffraction Laboratory Manager. The School of
Earth and Environmental Sciences, Washington
State University, Pullman is seeking a full time
laboratory manager.

The Scientific Laboratory Managerís
responsibilities will include oversight and
routine maintenance and operation of the JEOL
8500F electron microprobe and the Siemens D500
X-ray powder diffractometer and relevant sample
preparation facilities, sample preparation/sample
preparation advice, billing customers for
services, and course instruction. More
specifically, the operational responsibilities
will include: setting up the instruments and
monitoring their performance for customers/users
collecting their own data; data collection and
reduction, analysis for some customers, and
instrument demonstrations for classes and
visitors. In addition to instructing users in
the operation of the instruments, the Manager
will also be responsible for lectures and
laboratory exercises in Geology 552, X-ray
Analysis in Geology. The Manager will be
expected to attend X-ray diffraction and electron
microprobe workshops and meetings to keep abreast
of new technical developments and to provide
technical advice to faculty and students
preparing grant proposals involving the use of
the equipment. In addition, the Manager will be
expected to conduct their own research and seek
extramural funding.

Successful candidates are required to have a
Bachelorís degree and a background in electron
microprobe and/or X-ray powder diffraction
analysis. The Manager should ideally have a
Ph.D. in a physical science, preferably in
geosciences. Screening for this position will
begin on October 26, 2009 and continue until
filled. To apply, visit www.wsujobs.com.
Washington State University is an EO/AA Educator
and Employer.



Login Host: 134.121.47.46
---------------------------------------------------------------------------


==============================Original Headers==============================
11, 13 -- From zaluzec-at-microscopy.com Thu Nov 5 18:14:52 2009
11, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA60EpCa007073
11, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2009 18:14:52 -0600
11, 13 -- Mime-Version: 1.0
11, 13 -- Message-Id: {p06240807c7191aeacde0-at-[206.69.208.22]}
11, 13 -- Date: Thu, 5 Nov 2009 18:14:50 -0600
11, 13 -- To: microscopy-at-microscopy.com
11, 13 -- From: jawolff-at-mail.wsu.edu (by way of MicroscopyListserver)
11, 13 -- Subject: viaWWW: Job opening: electron microprobe operator
11, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
11, 13 -- Content-Transfer-Encoding: 8bit
11, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA60EpCa007073
==============================End of - Headers==============================




From: larry-at-cymru666.plus.com
Date: Fri, 6 Nov 2009 00:55:02 -0600
Subject: [Microscopy] Why coating is not required for TEM of polymer specimens.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On 04 Nov 2009, at 19:07, bozzola-at-siu.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Rajeev,
}
} Excellent question. Ultrathin (TEM) specimens are less prone to
} charging than bulk samples for several reasons: 1. less mass (as you
} already observed) to accommodate the static charge, 2. proximity to a
} metal (usually copper) grid that dissipates charges to ground.
} Nonetheless, TEM specimens do build up static charges, resulting in
} specimen drift or even ejection of smaller specimen parts, usually
} onto your objective aperture.
}
} If the specimen is in close proximity to a grid bar (or lying over a
} bar), usually there is little problem with static charging. However,
} with non-conducting, particulate specimens that are far away from a
} grid bar (and isolated), you will see major drifting, jumping and
} disappearance of the specimen due to charging. In that case, a light
} coating of carbon (by thermal evaporation) will usually solve the
} problem. Carbon substrates are less likely to show charging but they
} are fragile. Most people use Formvar/Carbon substrates, primarily for
} stability. Just make sure the specimen is on the carbonized side and
} that the carbon overlays the grid.
}
} I got a charge out of your question......
}
} John Bozzola
}
}
} --
} John J. Bozzola, Ph.D., Director
} Integrated Microscopy and Graphics Experts
} Southern Illinois University
} 750 Communications Drive
} Carbondale, IL 62901

There is also, in my opinion, a charge balancing effect. My
understanding is that the upper surface of the sample is charged by
incident electrons. With an objective aperture inserted just below the
sample, backscattered electrons from the aperture charge the lower
surface. The charge on the two surfaces tends to roughly balance,
stabilising the sample.

The demonstration that this is happening, it to load a polymer or
similar sample with no objective aperture inserted, or remove the
aperture after loading the sample. The sample tends to explode ......
especially with fresh samples. After having been exposed to the
electron beam for some time, samples seem to "harden" and become
resistant to exploding.

Regards,

Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoluk.com

(Working on a Microsoft-free computer)


==============================Original Headers==============================
8, 23 -- From larry-at-cymru666.plus.com Fri Nov 6 00:55:01 2009
8, 23 -- Received: from relay.pcl-ipout02.plus.net (relay.pcl-ipout02.plus.net [212.159.7.100])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA66t1Fx002564
8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Nov 2009 00:55:01 -0600
8, 23 -- X-IronPort-Anti-Spam-Filtered: true
8, 23 -- X-IronPort-Anti-Spam-Result: ApoEANZW80rUnw4U/2dsb2JhbADdP4Q9BA
8, 23 -- Received: from pih-relay08.plus.net ([212.159.14.20])
8, 23 -- by relay.pcl-ipout02.plus.net with ESMTP; 06 Nov 2009 06:55:01 +0000
8, 23 -- Received: from [87.115.146.70] (helo=[10.175.1.2])
8, 23 -- by pih-relay08.plus.net with esmtp (Exim) id 1N6Iiq-0002Az-Ol
8, 23 -- for Microscopy-at-microscopy.com; Fri, 06 Nov 2009 06:55:00 +0000
8, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
8, 23 -- Mime-Version: 1.0 (Apple Message framework v1076)
8, 23 -- Subject: Re: [Microscopy] RE: Why coating is not required for TEM of polymer specimens.
8, 23 -- From: Larry Stoter {larry-at-cymru666.plus.com}
8, 23 -- In-Reply-To: {200911041907.nA4J7Gkm014727-at-ns.microscopy.com}
8, 23 -- Date: Fri, 6 Nov 2009 06:55:00 +0000
8, 23 -- Content-Transfer-Encoding: 7bit
8, 23 -- Message-Id: {98CA14DC-DFA6-4C44-A322-C253213889E3-at-cymru666.plus.com}
8, 23 -- References: {200911041907.nA4J7Gkm014727-at-ns.microscopy.com}
8, 23 -- To: MSA Microscopy List {Microscopy-at-microscopy.com}
8, 23 -- X-Mailer: Apple Mail (2.1076)
8, 23 -- X-Plusnet-Relay: 28dbe84fad83675152da3921c4d85887
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Fri, 6 Nov 2009 08:53:15 -0600
Subject: [Microscopy] RE: Why coating is not required for TEM of polymer specimens.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} There is also, in my opinion, a charge balancing effect. My
} understanding is that the upper surface of the sample is charged by
} incident electrons. With an objective aperture inserted just below the
} sample, backscattered electrons from the aperture charge the lower
} surface. The charge on the two surfaces tends to roughly balance,
} stabilising the sample.
}
} The demonstration that this is happening, it to load a polymer or
} similar sample with no objective aperture inserted, or remove the
} aperture after loading the sample. The sample tends to explode ......
} especially with fresh samples. After having been exposed to the
} electron beam for some time, samples seem to "harden" and become
} resistant to exploding.
}
} Regards,
}
} Larry Stoter
} JEOL (UK) Ltd
} tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail:
} larrys-at-jeoluk.com
}
} (Working on a Microsoft-free computer)


I like the explanation!

Thank you,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784


==============================Original Headers==============================
7, 25 -- From DusevichV-at-umkc.edu Fri Nov 6 08:53:15 2009
7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp1.exchange.umkc.edu [134.193.143.155])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA6ErD1S011925
7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Nov 2009 08:53:14 -0600
7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
7, 25 -- Fri, 6 Nov 2009 08:53:09 -0600
7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
7, 25 -- Content-class: urn:content-classes:message
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: text/plain;
7, 25 -- charset="us-ascii"
7, 25 -- Subject: RE: [Microscopy] Why coating is not required for TEM of polymer specimens.
7, 25 -- Date: Fri, 6 Nov 2009 08:53:08 -0600
7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB30901B579-at-KC-MSX1.kc.umkc.edu}
7, 25 -- In-Reply-To: {200911060655.nA66tgCl003416-at-ns.microscopy.com}
7, 25 -- X-MS-Has-Attach:
7, 25 -- X-MS-TNEF-Correlator:
7, 25 -- Thread-Topic: [Microscopy] Why coating is not required for TEM of polymer specimens.
7, 25 -- thread-index: AcperikZ3krK8UoAQGqIiSKa6Cs9EAAQmt/Q
7, 25 -- References: {200911060655.nA66tgCl003416-at-ns.microscopy.com}
7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
7, 25 -- To: {Microscopy-at-microscopy.com}
7, 25 -- X-OriginalArrivalTime: 06 Nov 2009 14:53:09.0530 (UTC) FILETIME=[DB2557A0:01CA5EF0]
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA6ErD1S011925
==============================End of - Headers==============================




From: avergason-at-tetracore.com
Date: Fri, 6 Nov 2009 18:12:15 -0600
Subject: [Microscopy] viaWWW: Keyboard Connector Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both avergason-at-tetracore.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: avergason-at-tetracore.com
Name: Amy Vergason

Organization: Tetracore, Inc.

Title-Subject: [Filtered] Keyboard Connector Board

Question: Hello Everyone,

I am in the process of adding some old Philips hardware to my CM-10
TEM. I have a Keyboard Connector Board (KCB) that is part of the
Quad Serial Interface Kit PW 6440/00. I already have a regular
keyboard connected to the computer for normal use. I was wondering
if any CM-10 or CM-20 users out there have this capability and if so,
what are you using it for. I am not sure if I should just scrap this
piece or hold onto it for future use.

Thanks!
Amy

Login Host: 66.3.246.66
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Fri Nov 6 18:12:15 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA70CEg2019346
8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 6 Nov 2009 18:12:15 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c71a6bbdc0bd-at-[206.69.208.22]}
8, 11 -- Date: Fri, 6 Nov 2009 18:12:13 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: avergason-at-tetracore.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Keyboard Connector Board
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kenikea-at-ornl.gov
Date: Fri, 6 Nov 2009 18:12:52 -0600
Subject: [Microscopy] viaWWW: Adjust GW chamberscope view

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kenikea-at-ornl.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kenikea-at-ornl.gov
Name: Edward A. Kenik

Organization: ORNL

Title-Subject: [Filtered] Adjust GW chamberscope view

Question: Does any one know if it is possible to slightly modify the
view of a GW chamberscope, type 47 on a JEOL 6500F. The camera looks
slightly higher in the chamber than we need. Doing EBSD in our
instrument with a 45 degree pre-tilted holder, we can not see if the
forward-scattered detector on our Hikari camera will hit parts of the
tilted stage. Probably a 5mm lower view would be very useful.
Thanks. ED

Login Host: 160.91.156.84
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Nov 6 18:12:52 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA70CokC019659
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 6 Nov 2009 18:12:52 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c71a6bf3cd98-at-[206.69.208.22]}
6, 11 -- Date: Fri, 6 Nov 2009 18:12:50 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: kenikea-at-ornl.gov (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Adjust GW chamberscope view
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: olivier.guise-at-sabic-ip.com
Date: Fri, 6 Nov 2009 18:13:22 -0600
Subject: [Microscopy] viaWWW: Brittle fracture vs. Ductile fracture for polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both olivier.guise-at-sabic-ip.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: olivier.guise-at-sabic-ip.com
Name: Olivier Guise

Organization: SABIC Innovative Plastics

Title-Subject: [Filtered] Brittle fracture vs. Ductile fracture for
polymer materials

Question: I am looking for a publication/textbook... that refers to
the preparation of polymer microscopy samples in ductile versus
brittle fashion to study the deformation of the material at the crack
tip. I think it is pretty well established that cryofracturing is
the preferred method vs. room-temperature fracturing for polymer
materials as the ductile breakage does result in surface deformation.
But is there a well known defining publication on this, a reference
on brittle vs. ductile for polymer materials to reduce / avoid
surface deformation?

Can you help?

Regards,

Olivier

Olivier Guise, PhD
SABIC Innovative Plastics
Lead Analytical Technologist
Global Microscopy Functional Leader

Login Host: 63.172.27.2
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Fri Nov 6 18:13:22 2009
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA70DKdD019978
10, 12 -- for {microscopy-at-microscopy.com} ; Fri, 6 Nov 2009 18:13:21 -0600
10, 12 -- Mime-Version: 1.0
10, 12 -- Message-Id: {p06240802c71a6c0ed3e4-at-[206.69.208.22]}
10, 12 -- Date: Fri, 6 Nov 2009 18:13:18 -0600
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: olivier.guise-at-sabic-ip.com (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Brittle fracture vs. Ductile fracture for polymer
10, 12 -- materials
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Mon, 9 Nov 2009 05:00:42 -0600
Subject: [Microscopy] Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi there!

I wanted to react to the message below from Jared and give my personal and totally subjective opinion.
As I understood it this list is not made to advertise products, however sellers are allowed to use it to give informations about the products they want to sell. This is all very well (and most of time the sellers are really enriching the discussion), as long as the informations are fair. With this message I have the feeling that we are dangerously approaching the limits of fairness.
Why? Because there is a very important information missing: due to the low voltage (5 kV), one has to use Ultra-ultrathin sections (not more than 50 nm if I remember well). To cut that thin is really a challenge and requires a highly qualified personal. I think this point should have been discussed because a benchtop SEM is usually seen as an educational tool because it requires few or no specimen preparation. This point quite opposes the LVEM5 to a benchtop SEM in this regard. (BTW the low voltage makes also EDX analysis with LVEM5 almost meaningless). This point is NOT a detail, it is a critical point to be discussed.
I want to add that I like the concept of the LVEM5 and my contributions in this list showed it clearly. This microscope is unique and probably fills a niche on his own. However this microscope has its applications and limitations and these are important informations which should be given to any potential client. Please consider that you are not selling apples or vegetables but technologically advanced (and expensive) material and that the potential users need to know their limitations too, in order to be able to make wise choices.

Best regards,

Stephane





----- Original Message ----
X-from: "jared-at-lv-em.com" {jared-at-lv-em.com}
To: nizets2-at-yahoo.com
Sent: Fri, October 30, 2009 6:13:31 PM

Hello,

Since this has been a frequent issue, I will take a brief moment to
comment. Here is a disclaimer - Delong is the company which develops
and markets the LVEM5.

Here a few points on the LVEM5 benchtop electron microscope;

- It is a benchtop design with no special power or cooling requirements.
- It is actually three microscopes in one: TEM  with optional SEM
and/or STEM and Electron Diffraction
- It has a high-brightness field emission Shottky emitter that
requires minimal maintenance
- It is capable of resolutions of around 2.5nm in all three imaging modes
- It has higher contrast: up to 10 times higher contrast than a
conventional TEM WITHOUT STAIN
- It is affordable: A fraction of the cost of conventional electron
microscopes (Starting at $135,000 USD)


Feel free to visit our website www.lv-em.com  to browse through some
of the images we have taken with the LVEM5. If you like, I can send
you the product brochure and official price card as a PDF by email.

Please do not hesitate to contact me for more information.


Have a good day,


----------------------------

Jared Lapkovsky
Sales and Applications Engineer

LVEM5 Team
Delong America Inc.

Montreal, Canada

514.904.1202 ext. 701
www.lv-em.com


Come meet us at:

MRS Fall Exhibit (Hynes Convention Center, Boston, MA) December 1 - 3, Booth 909


On Fri, Oct 30, 2009 at 5:46 AM, {cw23-at-ohm.york.ac.uk} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Field emission sources provide better spatial resolution than
} traditional thermionic sources and
} I believe Novelx is the only company offering a benchtop Field Emission SEM.
}
} Chris Walker
} Department of Electronics
} University of York,
} Heslington,
} York,
} YO10 5DD.
}
} ZZhang-at-uwyo.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).
} }
} } The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.
} }
} } It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.
} }
} } It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.
} }
} } Hope this helps.
} }
} } Zhaojie
} }
} }
} } Zhaojie Zhang, Ph.D.
} } Director, Microscopy Core Facility
} } Department of Zoology and Physiology
} } University of Wyoming
} } Laramie, WY 82071
} } zzhang-at-uwyo.edu
} } 307-766-3038
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
} } Sent: Thursday, October 29, 2009 11:14 AM
} } To: Z.J. Zhang
} } Subject: [Microscopy] Re: viaWWW: Benchtop SEM, biggest bang for buck??
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} }      I also would like to know about the performance of these bench-top SEM
} } instruments and how they compare with full-size ones. And, to have some
} } orientation on its price. I know actual quotations would depend on many
} } different factors and can differ from lab to lab, but just to get an idea of
} } which level of funds has one to get to afford them.
} }
} } Many thanks,
} }
} } Antonio D. Molina-Garcia
} }
} } Instituto del Frío (CSIC)
} } José Antonio Nováis, 10
} } Ciudad Universitaria
} } 28040 Madrid
} } España
} }
} } Tel  +34 915445607    Fax  +34 915493627    E-mail :   ifrm111-at-if.csic.es
} } http://www.if.csic.es/ingiind.htm
} }
} }
} } ----- Original Message -----
} } X-from: {jason.gillen-at-encorewire.com}
} } To: {ifrm111-at-if.csic.es}
} } Sent: Thursday, October 29, 2009 12:15 AM
} } Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??
} }
} }
} }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } } To  Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
} } } ---------------------------------------------------------------------------
} } } Remember this posting is most likely not from a Subscriber, so when
} } } replying
} } } please  copy  both jason.gillen-at-encorewire.com as well as   the
} } } MIcroscopy Listserver
} } } ---------------------------------------------------------------------------
} } }
} } } Email: jason.gillen-at-encorewire.com
} } } Name: Gillenium
} } }
} } } Organization: Industry
} } }
} } } Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??
} } }
} } } Question: New lab comming online and need experienced information on
} } } the "desktop" SEM units available now.  I have read literature on
} } } FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
} } } also has a unit that offers X-ray analysis as well at a substantially
} } } higher price ca. $200K USD.  I have never used one of these
} } } "benchtop" units and would appreciate any experience with them, who
} } } makes the best one for the price, limitations etc. etc.   Thanks
} } }
} } }
} }
} }
} } ==============================Original Headers==============================
} } 26, 29 -- From ZZhang-at-uwyo.edu Thu Oct 29 12:44:43 2009
} } 26, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
} } 26, 29 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9THih0Z027206
} } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:44:43 -0500
} } 26, 29 -- Received: from ponyexpress-ht2.uwyo.edu (ponyexpress-ht2.uwyo.edu [10.84.60.209])
} } 26, 29 --     by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n9THiZp0024405
} } 26, 29 --     (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
} } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 11:44:41 -0600 (MDT)
} } 26, 29 --     (envelope-from ZZhang-at-uwyo.edu)
} } 26, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht2
} } 26, 29 --  ([10.84.60.209]) with mapi; Thu, 29 Oct 2009 11:44:41 -0600
} } 26, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
} } 26, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } 26, 29 -- Date: Thu, 29 Oct 2009 11:44:45 -0600
} } 26, 29 -- Subject: RE: Benchtop SEM, biggest bang for buck??
} } 26, 29 -- Thread-Topic: Benchtop SEM, biggest bang for buck??
} } 26, 29 -- Thread-Index: AcpYuyw8wl6LJJ/ZShir1Dy+wAR+bgAARZMw
} } 26, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE95525A14-at-ponyexpress-mb2}
} } 26, 29 -- References: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } 26, 29 -- In-Reply-To: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } 26, 29 -- Accept-Language: en-US
} } 26, 29 -- Content-Language: en-US
} } 26, 29 -- X-MS-Has-Attach:
} } 26, 29 -- X-MS-TNEF-Correlator:
} } 26, 29 -- acceptlanguage: en-US
} } 26, 29 -- Content-Type: text/plain; charset="iso-8859-1"
} } 26, 29 -- MIME-Version: 1.0
} } 26, 29 -- Content-Transfer-Encoding: 8bit
} } 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9THih0Z027206
} } ==============================End of - Headers==============================
} }
}
}
} ==============================Original Headers==============================
} 4, 27 -- From cw23-at-ohm.york.ac.uk Fri Oct 30 04:37:09 2009
} 4, 27 -- Received: from mail.elec.york.ac.uk (mailgw0.elec.york.ac.uk [144.32.152.4])
} 4, 27 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9U9b84u024599
} 4, 27 --        for {Microscopy-at-Microscopy.Com} ; Fri, 30 Oct 2009 04:37:08 -0500
} 4, 27 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
} 4, 27 --        by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
} 4, 27 --        id 1N3nut-0005xF-BL
} 4, 27 --        for Microscopy-at-Microscopy.Com; Fri, 30 Oct 2009 09:37:07 +0000
} 4, 27 -- Message-ID: {4AEAB3B8.5050807-at-ohm.york.ac.uk}
} 4, 27 -- Date: Fri, 30 Oct 2009 09:36:56 +0000
} 4, 27 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
} 4, 27 -- Organization: Department of Electronics, The University of York
} 4, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
} 4, 27 -- MIME-Version: 1.0
} 4, 27 -- To: Microscopy-at-Microscopy.Com
} 4, 27 -- Subject: Re: Benchtop SEMs
} 4, 27 -- References: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} 4, 27 -- In-Reply-To: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 4, 27 -- Content-Transfer-Encoding: 8bit
} 4, 27 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
} 4, 27 -- X-YorkElec-MailScanner-ID: 1N3nut-0005xF-BL
} 4, 27 -- X-YorkElec-MailScanner: Found to be clean
} 4, 27 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
} 4, 27 -- X-YorkElec-MailScanner-Watermark: 1257500227.4277-at-qh3QwG3i4HoZQ9oz8IaMWg
} 4, 27 -- X-Spam-Status: No
} 4, 27 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
} ==============================End of - Headers==============================


==============================Original Headers==============================
21, 33 -- From jared-at-lv-em.com Fri Oct 30 12:10:15 2009
21, 33 -- Received: from washington.serverhostcenter.com (washington.serverhostcenter.com [80.251.16.2])
21, 33 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UHAF40025161
21, 33 --     for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 12:10:15 -0500
21, 33 -- Received: from mail-yx0-f189.google.com ([209.85.210.189])
21, 33 --     by washington.serverhostcenter.com with esmtpsa (TLSv1:RC4-MD5:128)
21, 33 --     (Exim 4.69)
21, 33 --     (envelope-from {jared-at-lv-em.com} )
21, 33 --     id 1N3uzM-0001yM-VL
21, 33 --     for microscopy-at-microscopy.com; Fri, 30 Oct 2009 12:10:13 -0500
21, 33 -- Received: by yxe27 with SMTP id 27so3043663yxe.10
21, 33 --        for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 10:10:12 -0700 (PDT)
21, 33 -- MIME-Version: 1.0
21, 33 -- Received: by 10.101.138.19 with SMTP id q19mr1979304ann.99.1256922611852; Fri,
21, 33 --     30 Oct 2009 10:10:11 -0700 (PDT)
21, 33 -- In-Reply-To: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
21, 33 -- References: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
21, 33 -- Date: Fri, 30 Oct 2009 13:10:11 -0400
21, 33 -- Message-ID: {3629bb860910301010y11f317f6wb25615fca5e5e9d8-at-mail.gmail.com}
21, 33 -- Subject: Re: [Microscopy] Re: Benchtop SEMs
21, 33 -- From: Jared Lapkovsky {jared-at-lv-em.com}
21, 33 -- To: cw23-at-ohm.york.ac.uk, microscopy-at-microscopy.com
21, 33 -- Content-Type: text/plain; charset=ISO-8859-1
21, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
21, 33 -- X-AntiAbuse: Primary Hostname - washington.serverhostcenter.com
21, 33 -- X-AntiAbuse: Original Domain - microscopy.com
21, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
21, 33 -- X-AntiAbuse: Sender Address Domain - lv-em.com
21, 33 -- X-Source:
21, 33 -- X-Source-Args:
21, 33 -- X-Source-Dir:
21, 33 -- Content-Transfer-Encoding: 8bit
21, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9UHAF40025161
==============================End of - Headers==============================






==============================Original Headers==============================
39, 25 -- From nizets2-at-yahoo.com Mon Nov 9 05:00:41 2009
39, 25 -- Received: from web110807.mail.gq1.yahoo.com (web110807.mail.gq1.yahoo.com [67.195.13.230])
39, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA9B0fNN021606
39, 25 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 05:00:41 -0600
39, 25 -- Received: (qmail 91242 invoked by uid 60001); 9 Nov 2009 11:00:40 -0000
39, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1257764440; bh=xQzZ1fMh7yK8b+0OLVN8WbAf3pkJEfUFINj9amoXjhs=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jVGNpQIUikkwwmGPvj42y4ESNlFhhVVPY/7H/X1LSmAG+V74bcPIEiEIL6llTFfvU1i6FF5gVHxS8ux3VVdihFTpoB1T7RBU49/wHAwxb5P/1hEfkBBJi1i5S14THodU/RAnCOL8qrMUKP+pa4dAH4lpGkmXG3bAbdGv0MW/FsM=
39, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
39, 25 -- s=s1024; d=yahoo.com;
39, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
39, 25 -- b=YDETCj7Aut4O1ATFZJefKX5YsN0FbHhtocXMA5d2JcL9Ol98augbGdelGEbDMyAIEgPne1GKiMlGKrPkiV7ykWxPnWKUVLLbf0MG5P8ZcKSL1qaIVJd3QkA7+E8IXdsWVLtzB3rSjpogzEo4+RkqXbJBKSY+P9CC5546Valdal8=;
39, 25 -- Message-ID: {890969.85788.qm-at-web110807.mail.gq1.yahoo.com}
39, 25 -- X-YMail-OSG: ZVBqjv8VM1k.CmiV.04viduZ78DAMUKiHypI2TcUf45U_NlJUYOa4T2CFmdz_h9EaGh728TQT0cWYVJbbVLnOjbzUrVN6IKbCIteBW5kt_rC7xKWS.EbUgdQOmlITenp3bQuL9rn5Fq3ZZ5.mvK8sxChYWW0YWVEwky0j6nOjy6MpFtR3X6Sy8At0faniJuuUOOzGbuiPIgm1OVzs3Z34fpyRe0EoHlcb7sRh6OsXGLMQS4Yjtzyw_DdhXiUX7LRLPLGLad7tgDa5tvPco_sCqmD8XipuIviaRmZEwW6fvm1aPMHMp0IXuG7Y6Icr8W2Oc_aZlcuTAheBJcVj3P0WWOQoxnOubDkZxc_SvuHsvc10JwelDzgSJyj9w--
39, 25 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Mon, 09 Nov 2009 03:00:40 PST
39, 25 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
39, 25 -- References: {200910301713.n9UHDVYb031486-at-ns.microscopy.com}
39, 25 -- Date: Mon, 9 Nov 2009 03:00:40 -0800 (PST)
39, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
39, 25 -- Subject: Re: [Microscopy] Benchtop SEMs
39, 25 -- To: microscopy-at-microscopy.com
39, 25 -- Cc: jason.gillen-at-encorewire.com
39, 25 -- In-Reply-To: {200910301713.n9UHDVYb031486-at-ns.microscopy.com}
39, 25 -- MIME-Version: 1.0
39, 25 -- Content-Type: text/plain; charset=iso-8859-1
39, 25 -- Content-Transfer-Encoding: 8bit
39, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA9B0fNN021606
==============================End of - Headers==============================




From: beaurega-at-westol.com
Date: Mon, 9 Nov 2009 17:19:59 -0600
Subject: [Microscopy] Re: viaWWW: Keyboard Connector Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

The "SEM" keyboard is used to type overlay information onto "captured"
scanned images when yo have the STEM option. I am not sure a CM-10 had a
STEM option but maybe it was available. Anyway, what you type ultimately
ends up on the camera stand's Polaroid film exposure and image. The
characters are also displayed as you type on the dual STEM/SEM CRTs.

The keyboard plugs in on the far right side near the Polaroid camera stand,
as I recall. I hardly ever used that keyboard. It didn't have the power
of the PC's keyboard and computer to up load or down load software. The
keyboard is not a "must have" option but the Quad interface is worth having.

Good luck.

Paul

At 06:14 PM 11/6/09 -0600, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
6, 26 -- From beaurega-at-westol.com Mon Nov 9 17:19:59 2009
6, 26 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16])
6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nA9NJx1R013852
6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 17:19:59 -0600
6, 26 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10])
6, 26 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id nA9NJoTl028639
6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 18:19:51 -0500
6, 26 -- Received: (qmail 19775 invoked by uid 89); 9 Nov 2009 23:19:42 -0000
6, 26 -- Received: from pitts-69-72-117-13.dynamic-dialup.coretel.net (HELO running) (69.72.117.13)
6, 26 -- by mail.winbeam.com with SMTP; 9 Nov 2009 23:19:42 -0000
6, 26 -- Message-Id: {3.0.6.32.20091109182005.007e1250-at-mail.westol.com}
6, 26 -- X-Sender: beaurega-at-mail.westol.com (Unverified)
6, 26 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
6, 26 -- Date: Mon, 09 Nov 2009 18:20:05 -0500
6, 26 -- To: avergason-at-tetracore.com, microscopy-at-microscopy.com
6, 26 -- From: Beaurega {beaurega-at-westol.com}
6, 26 -- Subject: Re: [Microscopy] viaWWW: Keyboard Connector Board
6, 26 -- In-Reply-To: {200911070014.nA70EV9n021028-at-ns.microscopy.com}
6, 26 -- Mime-Version: 1.0
6, 26 -- Content-Type: text/plain; charset="us-ascii"
6, 26 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information
6, 26 -- X-Winbeam-MailScanner-ID: nA9NJoTl028639
6, 26 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner)
6, 26 -- X-Winbeam-MailScanner-SpamCheck: not spam, SpamAssassin (not cached,
6, 26 -- score=-1.32, required 4, AWL 0.68, BAYES_00 -2.00)
6, 26 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com
==============================End of - Headers==============================




From: pal.baggethun-at-elkem.no
Date: Mon, 9 Nov 2009 19:18:09 -0600
Subject: [Microscopy] viaWWW: LM: Stage Z calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pal.baggethun-at-elkem.no as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pal.baggethun-at-elkem.no
Name: P. Baggethun

Organization: Elkem Solar

Title-Subject: [Filtered] LM: Stage Z calibration standard

Question: Does anyone know where one may purchase a distance
calibration standard for the z movement (focus) of light-optical
microscope stages (preferably reflected light)?


Login Host: 212.4.33.171
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Nov 9 19:18:09 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAA1I8wS000573
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 19:18:09 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c71e6fc1b67d-at-[206.69.208.22]}
7, 11 -- Date: Mon, 9 Nov 2009 19:18:07 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: pal.baggethun-at-elkem.no (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: LM: Stage Z calibration standard
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: joonhyuk-at-umd.edu
Date: Mon, 9 Nov 2009 19:18:43 -0600
Subject: [Microscopy] viaWWW: Convergent beam electron diffraction (CBD) Software?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both joonhyuk-at-umd.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: joonhyuk-at-umd.edu
Name: JoonHyuk Yang

Organization: University of Maryland

Title-Subject: [Filtered] Convergent beam electron diffraction (CBD) Software?

Question: Dear All,

Hi, My name is Joon from University of Maryland.
I am trying to compare convergent beam electron diffraction (CBD)
pattern vs simulation results of oxide thin films.
I can not find CBD function in EMS web site (
http://cecm.insa-lyon.fr/CIOL/ ).
Is there any web site or free software I can simulate CBD pattern.
Please help me and reply me.

Thank you.

Sincerely,

Joon

Login Host: 129.2.42.29
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Nov 9 19:18:43 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAA1Igkb001293
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 19:18:42 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c71e6fd8bbf1-at-[206.69.208.22]}
10, 11 -- Date: Mon, 9 Nov 2009 19:18:40 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: joonhyuk-at-umd.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Convergent beam electron diffraction (CBD) Software?
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: riba-at-umd.edu
Date: Mon, 9 Nov 2009 19:19:51 -0600
Subject: [Microscopy] viaWWW: TEM CBED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both riba-at-umd.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: riba-at-umd.edu
Name: Lourdes Salamanca-Riba

Organization: University of Maryland

Title-Subject: [Filtered] TEM CBED

Question: Does anyone know of a good software to simulate CBED patterns?

Login Host: 129.2.175.79
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Nov 9 19:19:51 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAA1JndI004834
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 19:19:50 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c71e7026ce32-at-[206.69.208.22]}
6, 11 -- Date: Mon, 9 Nov 2009 19:19:49 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: riba-at-umd.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM CBED
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: stefan.geimer-at-uni-bayreuth.de
Date: Tue, 10 Nov 2009 03:21:02 -0600
Subject: [Microscopy] Anti-HA-Tag Antibody for Immunogold TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I am looking for an anti-HA-tag antibody which works well in immunogold
(plant tissue, chemical fix, embedded in LR-Gold).
Any suggestions?

Thanks a lot and all the best,

Stefan




*****************************************
Stefan Geimer, Ph.D.
Universitaet Bayreuth
Biologie/Elektronenmikroskopie NW I / B1
Universitaetsstr. 30
95447 Bayreuth
Germany
*****************************************



==============================Original Headers==============================
10, 24 -- From stefan.geimer-at-uni-bayreuth.de Tue Nov 10 03:21:02 2009
10, 24 -- Received: from btr0xn-tx.rz.uni-bayreuth.de (btr0xn.rz.uni-bayreuth.de [132.180.8.26])
10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAA9L0hr001439
10, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 03:21:01 -0600
10, 24 -- Received: from localhost (localhost [127.0.0.1])
10, 24 -- by btr0xn-tx.rz.uni-bayreuth.de (8.13.1/8.13.1) with ESMTP id nAA9KxBH017374
10, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 10:21:00 +0100 (MET)
10, 24 -- Received: from btr0xn-rx.rz.uni-bayreuth.de ([127.0.0.1])
10, 24 -- by localhost (mailhub-out.uni-bayreuth.de [127.0.0.1]) (amavisd-new, port 10024)
10, 24 -- with ESMTP id 17046-01-4 for {Microscopy-at-microscopy.com} ;
10, 24 -- Tue, 10 Nov 2009 10:20:49 +0100 (MET)
10, 24 -- Received: from [132.180.61.142] (btbzk5 [132.180.61.142])
10, 24 -- by btr0xn-rx.rz.uni-bayreuth.de (8.13.1/8.13.1) with ESMTP id nAA9IpBk016728
10, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 10:18:52 +0100 (MET)
10, 24 -- Message-ID: {4AF93001.4030207-at-uni-bayreuth.de}
10, 24 -- Date: Tue, 10 Nov 2009 10:18:57 +0100
10, 24 -- From: Stefan Geimer {stefan.geimer-at-uni-bayreuth.de}
10, 24 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 24 -- MIME-Version: 1.0
10, 24 -- To: Microscopy-at-microscopy.com
10, 24 -- Subject: Anti-HA-Tag Antibody for Immunogold TEM
10, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 24 -- Content-Transfer-Encoding: 7bit
10, 24 -- X-Virus-Scanned: amavisd-new at uni-bayreuth.de
==============================End of - Headers==============================




From: pwf-at-fullam.com
Date: Tue, 10 Nov 2009 08:22:25 -0600
Subject: [Microscopy] Microscopy books available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,


We are restructuring and no longer need various microscopy books. We are
offering these to the members of the listserve for the cost of shipping.


Here is the list:

M&M Proceedings 2007

Handbook of Chemistry and Physics 1935

Hitachi Electron Micrographs 1962? & earlier (2)

Electron Fractography Handbook- Phillips 1965

Images of Materials by Williams et al 1991

Conservation of Energy Resources NY Acad of Science Vol 324

8th TX Symposium on Rel Astrophysics NY Acad of Science Vol 302

Short ë Microscopy NY Acad of Science Vol 306

Glass Transition and Applications NY Acad of Science Vol 342

Transactions of American Xtalographic Assoc. Vol 14 1978

Particulate Clouds: Dusts, Smoke & Mists - Green and Lane 1957

Introduction to Solids Azaroff 1960

Fracture by Ed. Leibowitz Vol 1 1968

Science of Advanced Materials ASM Intl 1988 Seminar

Tables for Conversion of XRD Angles to Interplanar Spacing - Dept of
Commerce 1950

Factor Analysis in Chemistry by Maunowski 1980

Analytical Methods Applied to Air Pollution Measurements by Steven and Her
get 1974

The New World 1939/1946 Hewell and Anderson 1962

Instrument and Chemical Analysis Aspects of Electron Micro analysis by Elion
1966

Introduction to Electron Microscopy by Hall 1953 (2)

Engineering Alloys by Woldman 1959

Electroplating Engineering Handbook by Graham 1971

Merck Index 1960

Methods of Experimental Physics

Vol 1 Classical Methods by Esterman 1959

Vol 2 Electronic Methods by Bleuler 1964

Vol 3 Molecular Physics by Williams 1962

Vol 4 Atom & Electron Physics by Hughes 1967

Vol 5 Nuclear Physics Part A by Yuan 1961

Vol 5 Nuclear Physics Part B by Yuan 1963

Vol 6 Solid State Physics Part A by Horowitz 1959

Vol 6 Solid State Physics Part B by Horowitz 1959

Microbeam Analysis 1980 Wittry MAS Proceedings

Microbeam Analysis 1985 Armstrong MAS Proceedings

X-Ray Powder File Index 1960

Micron, Quarterly Journal June 1969, Sept 1969, Dec 1969

International Tables for Xray Crystallography Vol I 1965, Vol II 1967, Vol
III 1962

Stereos can Colloquium Proceedings 1969, 1970, 1972

ASTM Standards Part 31 and 32 May 1968

ASTM Standards Part 30 July 1971 and Part 30 July 1973

Radiotron Designers Handbook RCA Corp 1946

Science and Society- a Symposium by Stevenson et al 1965

Synthesis and Properties of Low Dimension Materials, NY Academy of Science
1978

Practical Papermaking by Clapperton 1929

Visual Lines for Spectrum Analysis by Smith 1928

Electron Microscopy 1980 Physics Eurem Proceedings

Tag Manual for Inspectors of Petroleum by Wilhelm 1939

Platinum Metals Review Vol 36 1992

Ferrous Metallurgy Vol III Metallography & Heat Treatment 1944

Molybdenum- Steels Irons Alloys by Archer et al 1953

Advances in Electronics and Physics by Marton 1960

Physics of Metals- Metallurgy and Met Eng Seitz 1943

Nuclear Fuels - Beckerly et al 1956

Qualitative Analysis by Long et al 1926

Reactor Handbook Vol II Fuel Reprocessing by Stoller 1961

High Temperature Oxidation of Metals by Kofstad 1966

Random House Encyclopedia 1983

Handbook of Chemistry and Physics, 29th ed 1945, Chemical Rubber Publishing
Co.

Cargille Data Sheets by Cargille Scientific ca 1965

Scanning Electron Microscopy an introduction by Eastern Analytical
Laboratories 1983 with 6 cassettes


Please contact us at sales-at-fullam.com with your shipping address and which
books you are interested in.


Dianne Fullam
Ernest F. Fullam, Inc



==============================Original Headers==============================
69, 31 -- From pwf-at-fullam.com Tue Nov 10 08:22:25 2009
69, 31 -- Received: from gateway10.websitewelcome.com (gateway10.websitewelcome.com [69.56.195.3])
69, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAAEMP6C001536
69, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 08:22:25 -0600
69, 31 -- Received: (qmail 858 invoked from network); 10 Nov 2009 14:35:03 -0000
69, 31 -- Received: from gator454.hostgator.com (74.53.140.18)
69, 31 -- by gateway10.websitewelcome.com with SMTP; 10 Nov 2009 14:35:03 -0000
69, 31 -- Received: from static-72-10-197-35.albyny.csvoip.net ([72.10.197.35]:50643 helo=diane)
69, 31 -- by gator454.hostgator.com with esmtpa (Exim 4.69)
69, 31 -- (envelope-from {pwf-at-fullam.com} )
69, 31 -- id 1N7rby-0001K2-2b; Tue, 10 Nov 2009 08:22:22 -0600
69, 31 -- From: "Peter Fullam" {pwf-at-fullam.com}
69, 31 -- To: {microscopy-at-microscopy.com}
69, 31 -- References: {200911100121.nAA1Lw2Y011912-at-ns.microscopy.com}
69, 31 -- Subject: Microscopy books available
69, 31 -- Date: Tue, 10 Nov 2009 09:22:22 -0500
69, 31 -- Message-ID: {DA2BE898C3864DB48BAC134ABC1E66C8-at-diane}
69, 31 -- MIME-Version: 1.0
69, 31 -- Content-Type: text/plain;
69, 31 -- charset="iso-8859-7"
69, 31 -- X-Mailer: Microsoft Office Outlook 11
69, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
69, 31 -- In-Reply-To: {200911100121.nAA1Lw2Y011912-at-ns.microscopy.com}
69, 31 -- Thread-Index: AcphpC9TPv1Ww1b4Q/Sv3xWEELKchwAbCsAg
69, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
69, 31 -- X-AntiAbuse: Primary Hostname - gator454.hostgator.com
69, 31 -- X-AntiAbuse: Original Domain - microscopy.com
69, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
69, 31 -- X-AntiAbuse: Sender Address Domain - fullam.com
69, 31 -- Content-Transfer-Encoding: 8bit
69, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAAEMP6C001536
==============================End of - Headers==============================




From: vcsolomon-at-ysu.edu
Date: Tue, 10 Nov 2009 19:24:44 -0600
Subject: [Microscopy] viaWWW: Faculty position at the Youngstown State University, Ohio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both vcsolomon-at-ysu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: vcsolomon-at-ysu.edu
Name: Virgil C. Solomon

Organization: Youngstown State University

Title-Subject: [Filtered] Faculty position at the Youngstown State
University, Ohio

Question: Youngstown State University
College of Science, Technology, Engineering & Mathematics (STEM)

Assistant/Associate Professor position

Tenure-track position is available in the Department of Chemistry to
support our growing emphasis in materials science. The successful
candidate will have prior experience in the development and
characterization of advanced materials (ceramics, metals and
ceramic-metallic nanocomposites). The department is very well
equipped in materials characterization instrumentation, such as X-ray
diffraction and electron microscopy (TEM, FIB/SEM). Participation in
the development of a Ph.D. program in Materials science and
Engineering will be major component of the position. Preference will
be given to candidates who are experienced with manufacturing
techniques for the production of ceramic and metallic materials,
particularly the relationships between material properties and
process parameters. Establishing an externally funded
interdisciplinary research program involving undergraduate and
graduate students is expected, as is collaborations with local
industrial companies. Teaching responsibilities may include both
undergraduate courses in inorganic and/or materials chemistry and
graduate courses in materials science.

MINIMUM QUALIFICATIONS: Ph.D. in Materials Science

SALARY: Commensurate with qualifications and experience

DATE AVAILABLE: August 16, 2010

CLOSING DATE FOR APPLICATIONS: Review of applications will begin
December 1, 2009, and continue until position is filled.
Applicants must send preferably as pdf files (1) a letter of
interest, (2) a current vita with employment history and dates, (3) a
copy of your transcript* documenting academic qualifications for this
position, and (4) three references which include the names,
addresses, phone numbers or e-mail addresses to:
Sherri Lovelace-Cameron, Search Committee Chair
Youngstown State University
One University Plaza
Youngstown OH 44555-0001
Phone: (330) 941-1997
e-mail: srlovela-at-cc.ysu.edu


Position announcement can be found at:
http://cfweb.cc.ysu.edu/hr/Position_Announcements.htm


Login Host: 150.134.100.226
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Tue Nov 10 19:24:44 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAB1Oh6j017622
15, 11 -- for {microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 19:24:44 -0600
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240802c71fc2c91be7-at-[206.69.208.22]}
15, 11 -- Date: Tue, 10 Nov 2009 19:24:42 -0600
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: vcsolomon-at-ysu.edu (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Faculty position at the Youngstown State University, Ohio
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ek1inc-at-msn.com
Date: Tue, 10 Nov 2009 19:25:21 -0600
Subject: [Microscopy] viaWWW: pore size of diatomite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ek1inc-at-msn.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ek1inc-at-msn.com
Name: steve adlman

Organization: coolit inc

Title-Subject: [Filtered] host material

Question: we are trying to find the pore size of diatomite both fresh
and salt water. we would like to use the material as host for lithium
chloride in a cooling a cooling device that we manufacture. any
suggestions you could make would be welcome----thanks---steve

Login Host: 75.84.211.180
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Nov 10 19:25:20 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAB1PG9U018261
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 19:25:19 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c71fc2ed2475-at-[206.69.208.22]}
6, 11 -- Date: Tue, 10 Nov 2009 19:25:15 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: ek1inc-at-msn.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: pore size of diatomite
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 10 Nov 2009 19:33:40 -0600
Subject: [Microscopy] Re: viaWWW: pore size of diatomite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pls check out my article in Microscopy Today which
appeared late last year or early this year.

It had puntae dimensions for diatoms from different
locales.

gary g.


At 05:27 PM 11/10/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Tue Nov 10 19:33:39 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAB1XdvN004068
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 10 Nov 2009 19:33:39 -0600
9, 20 -- Message-Id: {200911110133.nAB1XdvN004068-at-ns.microscopy.com}
9, 20 -- Received: (qmail 24352 invoked from network); 10 Nov 2009 17:13:10 -0800
9, 20 -- Received: by simscan 1.1.0 ppid: 24349, pid: 24350, t: 0.0867s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 10 Nov 2009 17:13:10 -0800
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Tue, 10 Nov 2009 17:33:35 -0800
9, 20 -- To: ek1inc-at-msn.com
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: pore size of diatomite
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200911110127.nAB1RBSw022284-at-ns.microscopy.com}
9, 20 -- References: {200911110127.nAB1RBSw022284-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: jared-at-lv-em.com
Date: Wed, 11 Nov 2009 09:24:00 -0600
Subject: [Microscopy] Benchtop SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

In response to Stephane's comments I would like to add the following
corrections to his description of the limitations of the LVEM5.

1) Sample size for SEM is 3mm diameter and 1.5mm height. This
generally means that most SEM samples can be attached directly to the
SEM stubs. A 50nm sample section is only for TEM mode, and this of
course depends on the sample type.

2) The LVEM5 is not simply an educational tool. Most of our users are
research facilities taking advantage of the increased contrast that a
5kv electron source offers in TEM mode. This allows difficult
materials such as silks, antibodies and other proteins to be imaged
unstained in TEM mode.

3) We do not have EDX at all on the LVEM5. This instrument is purely
an imaging device - but it does image in three different modes (TEM,
SEM, STEM).


The 5kv electron source is really our corner stone.

----------------------------

Jared Lapkovsky
Sales and Applications Engineer

LVEM5 Team
Delong America Inc.

Montreal, Canada

514.904.1202 ext. 701
www.lv-em.com


Come meet us at:

MRS Fall Exhibit (Hynes Convention Center, Boston, MA) December 1 - 3, Booth 909


On Mon, Nov 9, 2009 at 6:13 AM, {nizets2-at-yahoo.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi there!
}
} I wanted to react to the message below from Jared and give my personal and totally subjective opinion.
} As I understood it this list is not made to advertise products, however sellers are allowed to use it to give informations about the products they want to sell. This is all very well (and most of time the sellers are really enriching the discussion), as long as the informations are fair. With this message I have the feeling that we are dangerously approaching the limits of fairness.
} Why? Because there is a very important information missing: due to the low voltage (5 kV), one has to use Ultra-ultrathin sections (not more than 50 nm if I remember well). To cut that thin is really a challenge and requires a highly qualified personal. I think this point should have been discussed because a benchtop SEM is usually seen as an educational tool because it requires few or no specimen preparation. This point quite opposes the LVEM5 to a benchtop SEM in this regard. (BTW the low voltage makes also EDX analysis with LVEM5 almost meaningless). This point is NOT a detail, it is a critical point to be discussed.
} I want to add that I like the concept of the LVEM5 and my contributions in this list showed it clearly. This microscope is unique and probably fills a niche on his own. However this microscope has its applications and limitations and these are important informations which should be given to any potential client. Please consider that you are not selling apples or vegetables but technologically advanced (and expensive) material and that the potential users need to know their limitations too, in order to be able to make wise choices.
}
} Best regards,
}
} Stephane
}
}
}
}
}
} ----- Original Message ----
} X-from: "jared-at-lv-em.com" {jared-at-lv-em.com}
} To: nizets2-at-yahoo.com
} Sent: Fri, October 30, 2009 6:13:31 PM
} Subject: [Microscopy] Benchtop SEMs
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello,
}
} Since this has been a frequent issue, I will take a brief moment to
} comment. Here is a disclaimer - Delong is the company which develops
} and markets the LVEM5.
}
} Here a few points on the LVEM5 benchtop electron microscope;
}
} - It is a benchtop design with no special power or cooling requirements.
} - It is actually three microscopes in one: TEM  with optional SEM
} and/or STEM and Electron Diffraction
} - It has a high-brightness field emission Shottky emitter that
} requires minimal maintenance
} - It is capable of resolutions of around 2.5nm in all three imaging modes
} - It has higher contrast: up to 10 times higher contrast than a
} conventional TEM WITHOUT STAIN
} - It is affordable: A fraction of the cost of conventional electron
} microscopes (Starting at $135,000 USD)
}
}
} Feel free to visit our website www.lv-em.com  to browse through some
} of the images we have taken with the LVEM5. If you like, I can send
} you the product brochure and official price card as a PDF by email.
}
} Please do not hesitate to contact me for more information.
}
}
} Have a good day,
}
}
} ----------------------------
}
} Jared Lapkovsky
} Sales and Applications Engineer
}
} LVEM5 Team
} Delong America Inc.
}
} Montreal, Canada
}
} 514.904.1202 ext. 701
} www.lv-em.com
}
}
} Come meet us at:
}
} MRS Fall Exhibit (Hynes Convention Center, Boston, MA) December 1 - 3, Booth 909
}
}
} On Fri, Oct 30, 2009 at 5:46 AM, {cw23-at-ohm.york.ac.uk} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Field emission sources provide better spatial resolution than
} } traditional thermionic sources and
} } I believe Novelx is the only company offering a benchtop Field Emission SEM.
} }
} } Chris Walker
} } Department of Electronics
} } University of York,
} } Heslington,
} } York,
} } YO10 5DD.
} }
} } ZZhang-at-uwyo.edu wrote:
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } The price for the bench-top SEM (based on the Hitachi -TM-1000) is around $70K (without the x-ray unit).
} } }
} } } The SEM has only one BSE detector (no secondary electron detector). The resolution is about 30 nm, comparing to 2-5 nm resolution of a full sized SEM. Magnification goes from 20-10,000X.
} } }
} } } It has a dry pump and TMP (again, based on my experience with Hitachi TM-1000). It works great for low magnification work. Metal coating is not necessary, which simplifies greatly for sample prep. I even tried with fresh plant leaves, which are "relatively wet". You simply cut a small piece of leaf and put it directly into the SEM (no drying, coating etc). You can get nice images in a few minutes.
} } }
} } } It is a great tool for teaching, and low magnification, low resolution work. You would need a full size SEM if you want to do more serious work.
} } }
} } } Hope this helps.
} } }
} } } Zhaojie
} } }
} } }
} } } Zhaojie Zhang, Ph.D.
} } } Director, Microscopy Core Facility
} } } Department of Zoology and Physiology
} } } University of Wyoming
} } } Laramie, WY 82071
} } } zzhang-at-uwyo.edu
} } } 307-766-3038
} } }
} } }
} } }
} } }
} } } -----Original Message-----
} } } X-from: ifrm111-at-if.csic.es [mailto:ifrm111-at-if.csic.es]
} } } Sent: Thursday, October 29, 2009 11:14 AM
} } } To: Z.J. Zhang
} } } Subject: [Microscopy] Re: viaWWW: Benchtop SEM, biggest bang for buck??
} } }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } }      I also would like to know about the performance of these bench-top SEM
} } } instruments and how they compare with full-size ones. And, to have some
} } } orientation on its price. I know actual quotations would depend on many
} } } different factors and can differ from lab to lab, but just to get an idea of
} } } which level of funds has one to get to afford them.
} } }
} } } Many thanks,
} } }
} } } Antonio D. Molina-Garcia
} } }
} } } Instituto del Frío (CSIC)
} } } José Antonio Nováis, 10
} } } Ciudad Universitaria
} } } 28040 Madrid
} } } España
} } }
} } } Tel  +34 915445607    Fax  +34 915493627    E-mail :   ifrm111-at-if.csic.es
} } } http://www.if.csic.es/ingiind.htm
} } }
} } }
} } } ----- Original Message -----
} } } X-from: {jason.gillen-at-encorewire.com}
} } } To: {ifrm111-at-if.csic.es}
} } } Sent: Thursday, October 29, 2009 12:15 AM
} } } Subject: [Microscopy] viaWWW: Benchtop SEM, biggest bang for buck??
} } }
} } }
} } }
} } } }
} } } } ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } } } To  Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } ----------------------------------------------------------------------------
} } } }
} } } } This Question/Comment was submitted to the Microscopy Listserver
} } } } using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
} } } } ---------------------------------------------------------------------------
} } } } Remember this posting is most likely not from a Subscriber, so when
} } } } replying
} } } } please  copy  both jason.gillen-at-encorewire.com as well as   the
} } } } MIcroscopy Listserver
} } } } ---------------------------------------------------------------------------
} } } }
} } } } Email: jason.gillen-at-encorewire.com
} } } } Name: Gillenium
} } } }
} } } } Organization: Industry
} } } }
} } } } Title-Subject: [Filtered] Benchtop SEM, biggest bang for buck??
} } } }
} } } } Question: New lab comming online and need experienced information on
} } } } the "desktop" SEM units available now.  I have read literature on
} } } } FEI's Phenom, Hitachi has a mini SEM or benchtop version and Evax
} } } } also has a unit that offers X-ray analysis as well at a substantially
} } } } higher price ca. $200K USD.  I have never used one of these
} } } } "benchtop" units and would appreciate any experience with them, who
} } } } makes the best one for the price, limitations etc. etc.   Thanks
} } } }
} } } }
} } }
} } }
} } } ==============================Original Headers==============================
} } } 26, 29 -- From ZZhang-at-uwyo.edu Thu Oct 29 12:44:43 2009
} } } 26, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32])
} } } 26, 29 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9THih0Z027206
} } } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 12:44:43 -0500
} } } 26, 29 -- Received: from ponyexpress-ht2.uwyo.edu (ponyexpress-ht2.uwyo.edu [10.84.60.209])
} } } 26, 29 --     by aspensprings.uwyo.edu (8.14.2/8.14.2) with ESMTP id n9THiZp0024405
} } } 26, 29 --     (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO)
} } } 26, 29 --     for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2009 11:44:41 -0600 (MDT)
} } } 26, 29 --     (envelope-from ZZhang-at-uwyo.edu)
} } } 26, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht2
} } } 26, 29 --  ([10.84.60.209]) with mapi; Thu, 29 Oct 2009 11:44:41 -0600
} } } 26, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu}
} } } 26, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } } 26, 29 -- Date: Thu, 29 Oct 2009 11:44:45 -0600
} } } 26, 29 -- Subject: RE: Benchtop SEM, biggest bang for buck??
} } } 26, 29 -- Thread-Topic: Benchtop SEM, biggest bang for buck??
} } } 26, 29 -- Thread-Index: AcpYuyw8wl6LJJ/ZShir1Dy+wAR+bgAARZMw
} } } 26, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BE95525A14-at-ponyexpress-mb2}
} } } 26, 29 -- References: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } } 26, 29 -- In-Reply-To: {200910291713.n9THDaC1017665-at-ns.microscopy.com}
} } } 26, 29 -- Accept-Language: en-US
} } } 26, 29 -- Content-Language: en-US
} } } 26, 29 -- X-MS-Has-Attach:
} } } 26, 29 -- X-MS-TNEF-Correlator:
} } } 26, 29 -- acceptlanguage: en-US
} } } 26, 29 -- Content-Type: text/plain; charset="iso-8859-1"
} } } 26, 29 -- MIME-Version: 1.0
} } } 26, 29 -- Content-Transfer-Encoding: 8bit
} } } 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9THih0Z027206
} } } ==============================End of - Headers==============================
} } }
} }
} }
} } ==============================Original Headers==============================
} } 4, 27 -- From cw23-at-ohm.york.ac.uk Fri Oct 30 04:37:09 2009
} } 4, 27 -- Received: from mail.elec.york.ac.uk (mailgw0.elec.york.ac.uk [144.32.152.4])
} } 4, 27 --        by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9U9b84u024599
} } 4, 27 --        for {Microscopy-at-Microscopy.Com} ; Fri, 30 Oct 2009 04:37:08 -0500
} } 4, 27 -- Received: from mmgpc05.ohm.york.ac.uk ([144.32.136.144])
} } 4, 27 --        by mail.elec.york.ac.uk [144.32.138.107]:25 with esmtpa auth_user=cw23 (Exim 4.69)
} } 4, 27 --        id 1N3nut-0005xF-BL
} } 4, 27 --        for Microscopy-at-Microscopy.Com; Fri, 30 Oct 2009 09:37:07 +0000
} } 4, 27 -- Message-ID: {4AEAB3B8.5050807-at-ohm.york.ac.uk}
} } 4, 27 -- Date: Fri, 30 Oct 2009 09:36:56 +0000
} } 4, 27 -- From: Chris Walker {cw23-at-ohm.york.ac.uk}
} } 4, 27 -- Organization: Department of Electronics, The University of York
} } 4, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
} } 4, 27 -- MIME-Version: 1.0
} } 4, 27 -- To: Microscopy-at-Microscopy.Com
} } 4, 27 -- Subject: Re: Benchtop SEMs
} } 4, 27 -- References: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} } 4, 27 -- In-Reply-To: {200910291752.n9THqIsO013962-at-ns.microscopy.com}
} } 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 4, 27 -- Content-Transfer-Encoding: 8bit
} } 4, 27 -- X-YorkElec-MailScanner-Information: Please contact the ISP for more information
} } 4, 27 -- X-YorkElec-MailScanner-ID: 1N3nut-0005xF-BL
} } 4, 27 -- X-YorkElec-MailScanner: Found to be clean
} } 4, 27 -- X-YorkElec-MailScanner-From: cw23-at-ohm.york.ac.uk
} } 4, 27 -- X-YorkElec-MailScanner-Watermark: 1257500227.4277-at-qh3QwG3i4HoZQ9oz8IaMWg
} } 4, 27 -- X-Spam-Status: No
} } 4, 27 -- X-YorkElec-SenderRef: cw23-at-ohm.york.ac.uk
} } ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 21, 33 -- From jared-at-lv-em.com Fri Oct 30 12:10:15 2009
} 21, 33 -- Received: from washington.serverhostcenter.com (washington.serverhostcenter.com [80.251.16.2])
} 21, 33 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n9UHAF40025161
} 21, 33 --     for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 12:10:15 -0500
} 21, 33 -- Received: from mail-yx0-f189.google.com ([209.85.210.189])
} 21, 33 --     by washington.serverhostcenter.com with esmtpsa (TLSv1:RC4-MD5:128)
} 21, 33 --     (Exim 4.69)
} 21, 33 --     (envelope-from {jared-at-lv-em.com} )
} 21, 33 --     id 1N3uzM-0001yM-VL
} 21, 33 --     for microscopy-at-microscopy.com; Fri, 30 Oct 2009 12:10:13 -0500
} 21, 33 -- Received: by yxe27 with SMTP id 27so3043663yxe.10
} 21, 33 --        for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2009 10:10:12 -0700 (PDT)
} 21, 33 -- MIME-Version: 1.0
} 21, 33 -- Received: by 10.101.138.19 with SMTP id q19mr1979304ann.99.1256922611852; Fri,
} 21, 33 --     30 Oct 2009 10:10:11 -0700 (PDT)
} 21, 33 -- In-Reply-To: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
} 21, 33 -- References: {200910300946.n9U9kOMV005927-at-ns.microscopy.com}
} 21, 33 -- Date: Fri, 30 Oct 2009 13:10:11 -0400
} 21, 33 -- Message-ID: {3629bb860910301010y11f317f6wb25615fca5e5e9d8-at-mail.gmail.com}
} 21, 33 -- Subject: Re: [Microscopy] Re: Benchtop SEMs
} 21, 33 -- From: Jared Lapkovsky {jared-at-lv-em.com}
} 21, 33 -- To: cw23-at-ohm.york.ac.uk, microscopy-at-microscopy.com
} 21, 33 -- Content-Type: text/plain; charset=ISO-8859-1
} 21, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
} 21, 33 -- X-AntiAbuse: Primary Hostname - washington.serverhostcenter.com
} 21, 33 -- X-AntiAbuse: Original Domain - microscopy.com
} 21, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
} 21, 33 -- X-AntiAbuse: Sender Address Domain - lv-em.com
} 21, 33 -- X-Source:
} 21, 33 -- X-Source-Args:
} 21, 33 -- X-Source-Dir:
} 21, 33 -- Content-Transfer-Encoding: 8bit
} 21, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n9UHAF40025161
} ==============================End of - Headers==============================
}
}
}
}
}
}
} ==============================Original Headers==============================
} 39, 25 -- From nizets2-at-yahoo.com Mon Nov  9 05:00:41 2009
} 39, 25 -- Received: from web110807.mail.gq1.yahoo.com (web110807.mail.gq1.yahoo.com [67.195.13.230])
} 39, 25 --       by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nA9B0fNN021606
} 39, 25 --       for {microscopy-at-microscopy.com} ; Mon, 9 Nov 2009 05:00:41 -0600
} 39, 25 -- Received: (qmail 91242 invoked by uid 60001); 9 Nov 2009 11:00:40 -0000
} 39, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1257764440; bh=xQzZ1fMh7yK8b+0OLVN8WbAf3pkJEfUFINj9amoXjhs=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jVGNpQIUikkwwmGPvj42y4ESNlFhhVVPY/7H/X1LSmAG+V74bcPIEiEIL6llTFfvU1i6FF5gVHxS8ux3VVdihFTpoB1T7RBU49/wHAwxb5P/1hEfkBBJi1i5S14THodU/RAnCOL8qrMUKP+pa4dAH4lpGkmXG3bAbdGv0MW/FsM=
} 39, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
} 39, 25 --   s=s1024; d=yahoo.com;
} 39, 25 --   h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
} 39, 25 --   b=YDETCj7Aut4O1ATFZJefKX5YsN0FbHhtocXMA5d2JcL9Ol98augbGdelGEbDMyAIEgPne1GKiMlGKrPkiV7ykWxPnWKUVLLbf0MG5P8ZcKSL1qaIVJd3QkA7+E8IXdsWVLtzB3rSjpogzEo4+RkqXbJBKSY+P9CC5546Valdal8=;
} 39, 25 -- Message-ID: {890969.85788.qm-at-web110807.mail.gq1.yahoo.com}
} 39, 25 -- X-YMail-OSG: ZVBqjv8VM1k.CmiV.04viduZ78DAMUKiHypI2TcUf45U_NlJUYOa4T2CFmdz_h9EaGh728TQT0cWYVJbbVLnOjbzUrVN6IKbCIteBW5kt_rC7xKWS.EbUgdQOmlITenp3bQuL9rn5Fq3ZZ5.mvK8sxChYWW0YWVEwky0j6nOjy6MpFtR3X6Sy8At0faniJuuUOOzGbuiPIgm1OVzs3Z34fpyRe0EoHlcb7sRh6OsXGLMQS4Yjtzyw_DdhXiUX7LRLPLGLad7tgDa5tvPco_sCqmD8XipuIviaRmZEwW6fvm1aPMHMp0IXuG7Y6Icr8W2Oc_aZlcuTAheBJcVj3P0WWOQoxnOubDkZxc_SvuHsvc10JwelDzgSJyj9w--
} 39, 25 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Mon, 09 Nov 2009 03:00:40 PST
} 39, 25 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
} 39, 25 -- References: {200910301713.n9UHDVYb031486-at-ns.microscopy.com}
} 39, 25 -- Date: Mon, 9 Nov 2009 03:00:40 -0800 (PST)
} 39, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 39, 25 -- Subject: Re: [Microscopy]  Benchtop SEMs
} 39, 25 -- To: microscopy-at-microscopy.com
} 39, 25 -- Cc: jason.gillen-at-encorewire.com
} 39, 25 -- In-Reply-To: {200910301713.n9UHDVYb031486-at-ns.microscopy.com}
} 39, 25 -- MIME-Version: 1.0
} 39, 25 -- Content-Type: text/plain; charset=iso-8859-1
} 39, 25 -- Content-Transfer-Encoding: 8bit
} 39, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nA9B0fNN021606
} ==============================End of - Headers==============================


==============================Original Headers==============================
18, 33 -- From jared-at-lv-em.com Wed Nov 11 09:24:00 2009
18, 33 -- Received: from washington.serverhostcenter.com (washington.serverhostcenter.com [80.251.16.2])
18, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nABFNxjt004274
18, 33 -- for {microscopy-at-microscopy.com} ; Wed, 11 Nov 2009 09:23:59 -0600
18, 33 -- Received: from mail-iw0-f187.google.com ([209.85.223.187])
18, 33 -- by washington.serverhostcenter.com with esmtpsa (TLSv1:RC4-MD5:128)
18, 33 -- (Exim 4.69)
18, 33 -- (envelope-from {jared-at-lv-em.com} )
18, 33 -- id 1N8F37-0000sz-UB
18, 33 -- for microscopy-at-microscopy.com; Wed, 11 Nov 2009 09:23:58 -0600
18, 33 -- Received: by iwn17 with SMTP id 17so926811iwn.10
18, 33 -- for {microscopy-at-microscopy.com} ; Wed, 11 Nov 2009 07:23:56 -0800 (PST)
18, 33 -- MIME-Version: 1.0
18, 33 -- Received: by 10.231.10.16 with SMTP id n16mr3100424ibn.24.1257953035960; Wed,
18, 33 -- 11 Nov 2009 07:23:55 -0800 (PST)
18, 33 -- In-Reply-To: {200911091113.nA9BDKG0003258-at-ns.microscopy.com}
18, 33 -- References: {200911091113.nA9BDKG0003258-at-ns.microscopy.com}
18, 33 -- Date: Wed, 11 Nov 2009 10:23:55 -0500
18, 33 -- Message-ID: {3629bb860911110723n4655dc29h868ffab78191bc2d-at-mail.gmail.com}
18, 33 -- Subject: Re: [Microscopy] Re: Benchtop SEMs
18, 33 -- From: Jared Lapkovsky {jared-at-lv-em.com}
18, 33 -- To: microscopy-at-microscopy.com
18, 33 -- Content-Type: text/plain; charset=ISO-8859-1
18, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
18, 33 -- X-AntiAbuse: Primary Hostname - washington.serverhostcenter.com
18, 33 -- X-AntiAbuse: Original Domain - microscopy.com
18, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
18, 33 -- X-AntiAbuse: Sender Address Domain - lv-em.com
18, 33 -- X-Source:
18, 33 -- X-Source-Args:
18, 33 -- X-Source-Dir:
18, 33 -- Content-Transfer-Encoding: 8bit
18, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nABFNxjt004274
==============================End of - Headers==============================




From: rhsia-at-umaryland.edu
Date: Wed, 11 Nov 2009 21:20:07 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: LKB ultramicrotom III

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rhsia-at-umaryland.edu
Name: Ru-ching Hsia

Organization: U maryland, EM Core Facility

Title-Subject: [Filtered] LKB ultramicrotom III cable

Question: Dear all,

One of our EM facility users is trying to set up an old LKB
ultramicrotome III type 8801A in his own lab. However, he found the
calbe connecting the power box to the back of the ultramicrotome is
damaged. Does anyone by chance have such a cable and is willing to
part with it?

Many thanks.

Ru-ching Hsia

Login Host: 134.192.86.174
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Wed Nov 11 21:20:07 2009
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAC3K5ao000612
9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 11 Nov 2009 21:20:06 -0600
9, 12 -- Mime-Version: 1.0
9, 12 -- Message-Id: {p06240801c7212f4a4903-at-[206.69.208.22]}
9, 12 -- Date: Wed, 11 Nov 2009 21:20:05 -0600
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver)
9, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: LKB ultramicrotom III
9, 12 -- cable
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: yaoz-at-me.queensu.ca
Date: Wed, 11 Nov 2009 21:20:47 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Faraday Cup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both yaoz-at-me.queensu.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: yaoz-at-me.queensu.ca
Name: zhongwen yao

Organization: Queen's University

Title-Subject: [Filtered] Faraday Cup

Question: Can anybody offer a stand alone Faraday cup working for
CM20? We can negotiate on price.

Thanks in advance.

ZW

Login Host: 130.15.116.234
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Nov 11 21:20:47 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAC3KjiJ001258
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 11 Nov 2009 21:20:46 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240802c7212f75531f-at-[206.69.208.22]}
8, 11 -- Date: Wed, 11 Nov 2009 21:20:45 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: yaoz-at-me.queensu.ca (by way of MicroscopyListserver)
8, 11 -- Subject: [Filtered] MicroscopyListserverviaWWW: Faraday Cup
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jason.gillen-at-encorewire.com
Date: Fri, 13 Nov 2009 07:59:11 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Optimal ESEM set up for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jason.gillen-at-encorewire.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jason.gillen-at-encorewire.com
Name: Jason Gillen

Organization: Encore

Title-Subject: [Filtered] Optimal ESEM set up for polymer analysis

Question: Have recently headed down the ESEM path chasing the
"perfect" set-up for polymer analysis. Any opinions on emmiter
sources (LaB6 or W whats best for polymers), optimal detectors (SiLi,
BSD,SE) and finaly whats everybodys favorite EDX software. ESEM is a
relatively new venture for me and I want the right combo for polymer
study.

Thanks for all the consistently helpful information this server produces...

Login Host: 74.2.250.50
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Fri Nov 13 07:59:10 2009
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nADDxAKh020117
7, 12 -- for {microscopy-at-microscopy.com} ; Fri, 13 Nov 2009 07:59:10 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- Message-Id: {p06240802c72316a09ec7-at-[206.69.208.22]}
7, 12 -- Date: Fri, 13 Nov 2009 07:59:09 -0600
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: jason.gillen-at-encorewire.com (by way of MicroscopyListserver)
7, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: Optimal ESEM set up for
7, 12 -- polymer analysis
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: marksmsa-at-gmail.com
Date: Sat, 14 Nov 2009 08:00:40 -0600
Subject: [Microscopy] Trying to trace some ex-students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jason, I do not normally suggest that SEM is great for polymer analysis,
but since that is what I have, I try to use it on polymers anyway.

The problem with polymers is that so many look about the same. They have
plenty of C (which we can see) and H (which we cannot). There are
varying levels of O. If other elements like Cl, F, or S are present,
they can be helpful in identifying the polymer (or at least narrowing
down the choices). Even if N is present it is usually hard to detect. If
falls between C and O, so you detector has to be on its best behavior. I
don't know that there is a big case for getting a new SDD detector for
use on polymers. The count rates are usually lower than for inorganic
materials. You do probably want to investigate and compare window
materials for various detectors. I don't have my notes handy, but some
are better for seeing N than are others.

Since those are all fairly light elements, you will probably want to
operate with low voltages. I would look for good performance below 5 kV.


You will want a good BSE detector. Even though we can't see H by EDS,
the H:C ratio will alter the BSE brightness. You should be able to
easily differentiate between polystyrene and polyethylene. Make sure
your detector works well at low voltages.

I don't know that you really need ESEM for polymers. We use a variable
pressure SEM (VP-SEM) well enough. However, having said that, we are
looking at an ESEM for other reasons.

Warren

-----Original Message-----
X-from: jason.gillen-at-encorewire.com [mailto:jason.gillen-at-encorewire.com]
Sent: Friday, November 13, 2009 8:00 AM
To: wesaia-at-iastate.edu

I am trying to trace some ex-students who I have lost contact me. If
you have any information, please email me directly at L-marks [at]
northwestern.edu

Lu-Chen -- I may have spelled his name wrong. He did a masters with me
back around 1991, moved to do his PhD with Lynn
Hobbs, did a brief postdoc at Argonne and is now faculty somewhere in
the US on the east coast.

Yiquin Ma. Did his PhD with me finishing in 1990, a postdoc with Jon
Gjonnes then Alwyn Eades then I believed did a masters in computer
science and moved to the financial sector.

Rebecca Ai. Did her PhD with me finishing in 1992, moved to Motorola
in Tempe, AZ and then...

Scott Savage. Did his masters with me finishing in (about) 1992, moved
to Motorola in Tempe, AZ and then ...

Dorai Narayanaswamy. Did his PhD with me finishing in 1995, a brief
postdoc at ANL then moved (I believe) to Motorola in Tempe, AZ and
then ...

Michael Carmody. Did his PhD with me and Karl Merkle, finishing in
2000, I believe moved to GE (or Applied Materials) on the West Coast
and then ...

Hong Zhang. Worked as a postdoc lleaving around 1995 to work at IBM in
the San Francisco area.

Peirong (Samantha) Xu. Worked as a postdoc leaving around 1995 to work
at Intel in the San Francisco area.

Ian Widlow. Left around 2004 to work at Applied Materials...

--
Professor Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60208, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L-marks at northwestern dot edu
Web: www.numis.northwestern.edu
EMM2007 http://ns.crys.ras.ru/EMMM07/
Commission on Electron Diffraction of IUCR
www.numis.northwestern.edu/IUCR_CED

==============================Original Headers==============================
11, 29 -- From marksmsa-at-gmail.com Sat Nov 14 08:00:40 2009
11, 29 -- Received: from mail-bw0-f224.google.com (mail-bw0-f224.google.com [209.85.218.224])
11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAEE0dDR008263
11, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 14 Nov 2009 08:00:39 -0600
11, 29 -- Received: by bwz24 with SMTP id 24so4539185bwz.10
11, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 14 Nov 2009 06:00:38 -0800 (PST)
11, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 29 -- d=gmail.com; s=gamma;
11, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
11, 29 -- :from:to:content-type;
11, 29 -- bh=YiocEwqXMkQUOeuwGSwYlc5M0/UGnPly8fPnAEbGNtY=;
11, 29 -- b=U3+rZ7tzjRnsoN9/jK+tKEzKE8kPp8KjqEDzuayj+4SptR/Y+7GzmRwwL9TKVJz6bB
11, 29 -- PyyeEoa4sl4/2BBRGcg0+aSzGhjrJK+VAJumdQwHIAQHm5/nx6PwezGYHrcq0Qb8OuBM
11, 29 -- UInGvOBmC8acGKoG5wN+0pYMRzWYCU5kyMCPQ=
11, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
11, 29 -- d=gmail.com; s=gamma;
11, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
11, 29 -- b=I/9fIjsenfM/WT2l0kkubdeYppWB/cAmrRGWjT5/RgRGgDJkJ2fE9oFOS/wvYBgsy+
11, 29 -- hF3k4ACEJA+APTslxqfdGxujwiylFvsRNv4trYZCrDG/ulHgAPNDLCKB3O4bmkH9S4cd
11, 29 -- 8+pfbsUaZKrC1ed7U93JNzyp31typqpiNZlXc=
11, 29 -- MIME-Version: 1.0
11, 29 -- Received: by 10.239.138.12 with SMTP id n12mr565031hbn.69.1258207236944; Sat,
11, 29 -- 14 Nov 2009 06:00:36 -0800 (PST)
11, 29 -- Date: Sat, 14 Nov 2009 08:00:36 -0600
11, 29 -- Message-ID: {e13ba6260911140600i5a7ae5e0ob2ad23df8f90f232-at-mail.gmail.com}
11, 29 -- Subject: Trying to trace some ex-students
11, 29 -- From: L Marks {marksmsa-at-gmail.com}
11, 29 -- To: microscopy {Microscopy-at-microscopy.com}
11, 29 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: marksmsa-at-gmail.com
Date: Sun, 15 Nov 2009 10:48:51 -0600
Subject: [Microscopy] Re: Trying to trace some ex-students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to those of you who have responded with information about
Hong Zhang (m) who works at Applied Materials; in fact I am looking
for information about Hong Zhang (f) who worked at IBM at least until
~2004 (and maybe still does) in the SF area.

The two of them were at Northwestern at the same time, and were even
co-authors on a paper. The editors asked for a way to differentiate
between them, then declined the only suggestion we have (i.e. (m) and
(f)). In hindsight we could have suggested height, i.e. (6' 2") and
(5' 4") but they probably would not have wanted that either.

On Sat, Nov 14, 2009 at 8:07 AM, {marksmsa-at-gmail.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I am trying to trace some ex-students who I have lost contact me. If
} you have any information, please email me directly at L-marks [at]
} northwestern.edu
}
} Lu-Chen -- I may have spelled his name wrong. He did a masters with me
} back around 1991, moved to do his PhD with Lynn
} Hobbs, did a brief postdoc at Argonne and is now faculty somewhere in
} the US on the east coast.
}
} Yiquin Ma. Did his PhD with me finishing in 1990, a postdoc with Jon
} Gjonnes then Alwyn Eades then I believed did a masters in computer
} science and moved to the financial sector.
}
} Rebecca Ai. Did her PhD with me finishing in 1992, moved to Motorola
} in Tempe, AZ and then...
}
} Scott Savage. Did his masters with me finishing in (about) 1992, moved
} to Motorola in Tempe, AZ and then ...
}
} Dorai Narayanaswamy. Did his PhD with me finishing in 1995, a brief
} postdoc at ANL then moved (I believe) to Motorola in Tempe, AZ and
} then ...
}
} Michael Carmody. Did his PhD with me and Karl Merkle, finishing in
} 2000, I believe moved to GE (or Applied Materials) on the West Coast
} and then ...
}
} Hong Zhang. Worked as a postdoc lleaving around 1995 to work at IBM in
} the San Francisco area.
}
} Peirong (Samantha) Xu. Worked as a postdoc leaving around 1995 to work
} at Intel in the San Francisco area.
}
} Ian Widlow. Left around 2004 to work at Applied Materials...
}
} --
} Professor Laurence Marks
} Department of Materials Science and Engineering
} MSE Rm 2036 Cook Hall
} 2220 N Campus Drive
} Northwestern University
} Evanston, IL 60208, USA
} Tel: (847) 491-3996 Fax: (847) 491-7820
} email: L-marks at northwestern dot edu
} Web: www.numis.northwestern.edu
} EMM2007 http://ns.crys.ras.ru/EMMM07/
} Commission on Electron Diffraction of IUCR
} www.numis.northwestern.edu/IUCR_CED
}
} ==============================Original Headers==============================
} 11, 29 -- From marksmsa-at-gmail.com Sat Nov 14 08:00:40 2009
} 11, 29 -- Received: from mail-bw0-f224.google.com (mail-bw0-f224.google.com [209.85.218.224])
} 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAEE0dDR008263
} 11, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 14 Nov 2009 08:00:39 -0600
} 11, 29 -- Received: by bwz24 with SMTP id 24so4539185bwz.10
} 11, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 14 Nov 2009 06:00:38 -0800 (PST)
} 11, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 11, 29 -- d=gmail.com; s=gamma;
} 11, 29 -- h=domainkey-signature:mime-version:received:date:message-id:subject
} 11, 29 -- :from:to:content-type;
} 11, 29 -- bh=YiocEwqXMkQUOeuwGSwYlc5M0/UGnPly8fPnAEbGNtY=;
} 11, 29 -- b=U3+rZ7tzjRnsoN9/jK+tKEzKE8kPp8KjqEDzuayj+4SptR/Y+7GzmRwwL9TKVJz6bB
} 11, 29 -- PyyeEoa4sl4/2BBRGcg0+aSzGhjrJK+VAJumdQwHIAQHm5/nx6PwezGYHrcq0Qb8OuBM
} 11, 29 -- UInGvOBmC8acGKoG5wN+0pYMRzWYCU5kyMCPQ=
} 11, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
} 11, 29 -- d=gmail.com; s=gamma;
} 11, 29 -- h=mime-version:date:message-id:subject:from:to:content-type;
} 11, 29 -- b=I/9fIjsenfM/WT2l0kkubdeYppWB/cAmrRGWjT5/RgRGgDJkJ2fE9oFOS/wvYBgsy+
} 11, 29 -- hF3k4ACEJA+APTslxqfdGxujwiylFvsRNv4trYZCrDG/ulHgAPNDLCKB3O4bmkH9S4cd
} 11, 29 -- 8+pfbsUaZKrC1ed7U93JNzyp31typqpiNZlXc=
} 11, 29 -- MIME-Version: 1.0
} 11, 29 -- Received: by 10.239.138.12 with SMTP id n12mr565031hbn.69.1258207236944; Sat,
} 11, 29 -- 14 Nov 2009 06:00:36 -0800 (PST)
} 11, 29 -- Date: Sat, 14 Nov 2009 08:00:36 -0600
} 11, 29 -- Message-ID: {e13ba6260911140600i5a7ae5e0ob2ad23df8f90f232-at-mail.gmail.com}
} 11, 29 -- Subject: Trying to trace some ex-students
} 11, 29 -- From: L Marks {marksmsa-at-gmail.com}
} 11, 29 -- To: microscopy {Microscopy-at-microscopy.com}
} 11, 29 -- Content-Type: text/plain; charset=ISO-8859-1
} ==============================End of - Headers==============================
}



--
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60208, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L-marks at northwestern dot edu
Web: www.numis.northwestern.edu
EMM2007 http://ns.crys.ras.ru/EMMM07/
Commission on Electron Diffraction of IUCR
www.numis.northwestern.edu/IUCR_CED

==============================Original Headers==============================
6, 32 -- From marksmsa-at-gmail.com Sun Nov 15 10:48:51 2009
6, 32 -- Received: from mail-bw0-f224.google.com (mail-bw0-f224.google.com [209.85.218.224])
6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAFGmooF006096
6, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Nov 2009 10:48:50 -0600
6, 32 -- Received: by bwz24 with SMTP id 24so5158395bwz.10
6, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Nov 2009 08:48:50 -0800 (PST)
6, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 32 -- d=gmail.com; s=gamma;
6, 32 -- h=domainkey-signature:mime-version:received:in-reply-to:references
6, 32 -- :date:message-id:subject:from:to:content-type;
6, 32 -- bh=vzoqH8bPi03ljvaWPFrGSOyMx4jFncFEh2x6OH1HnHE=;
6, 32 -- b=eRIdRL368CvI9BYMG4sq2kBv76m3rCiwbnx+83X0VLW0/iLTtdbbUcoX2cpmH7NgCJ
6, 32 -- B/Cz9D4Gopqnv+I4862/2ruQVW3AjC3vPZ1RbSyRDag10R1Trxa8MnGCWlLwS10zeqFC
6, 32 -- k6HOzuCg1eh35pRtmqsm1tA/U7L+d7CjI+Qo0=
6, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
6, 32 -- d=gmail.com; s=gamma;
6, 32 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to
6, 32 -- :content-type;
6, 32 -- b=vT+3A2TzjDihWxJH9+yeUAy5OA9SUU6wgmWhMWQSGlNyXKzv2uTbFT42nUPnxuNf8d
6, 32 -- opfhsrB8J7O2WoFUWlM5Bor44JZpIaq9RDNbtC7GgANochDomajWOwcDuIAPnbK8Uu4P
6, 32 -- NpP6tPKf+EHKbtXo71f2FN9Oxaphr1DrKnIkM=
6, 32 -- MIME-Version: 1.0
6, 32 -- Received: by 10.239.138.12 with SMTP id n12mr677675hbn.69.1258303729562; Sun,
6, 32 -- 15 Nov 2009 08:48:49 -0800 (PST)
6, 32 -- In-Reply-To: {200911141407.nAEE77AF016424-at-ns.microscopy.com}
6, 32 -- References: {200911141407.nAEE77AF016424-at-ns.microscopy.com}
6, 32 -- Date: Sun, 15 Nov 2009 10:48:49 -0600
6, 32 -- Message-ID: {e13ba6260911150848q267313ecr6defe34606173785-at-mail.gmail.com}
6, 32 -- Subject: Re: [Microscopy] Trying to trace some ex-students
6, 32 -- From: L Marks {marksmsa-at-gmail.com}
6, 32 -- To: microscopy {Microscopy-at-microscopy.com}
6, 32 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Mon, 16 Nov 2009 11:14:11 -0600
Subject: [Microscopy] FIB on bees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
today I tried to mill into various parts of a honey-bee with not much
success.
Please have a look at these images:
www.elektronenmikroskopie.info/beegroup/fib
Specimen had been critical-point-dried and sputtered with ca. 10 to 15nm Pt.
Milling had been with Gallium at 30KV and ca. 10 to 20 nA beam current.

First three images are showing a cut of one of the claws, sorry for the
missing close-up, which showed much curtaining. Depth is ca. 10um.

Next images had been a trial run at the bee`s eye with various depth of
milling: images 06 to 012 milled to a depth of ca. 1 um, images 13 to 16
milled to 200 nm depth.
I suppose, that original structure of the specimen is only to be seen at
images 010, 015 and 16, just below the Pt-layer, but it may also be an
artefact of redeposited Pt or Gallium.

Any ideas if image 07 is showing artefacts of the milling or some
structure of the bee-eye?

Did anybody do FIB on organic material and is willing to send me thge
procedure? Maybe a lower kv or current will give lesser artifacts?

Best regards,
Stefan



--
-----------------------------------------------------
Stefan Diller - Scientifc Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

==============================Original Headers==============================
10, 20 -- From stefan.diller-at-t-online.de Mon Nov 16 11:14:10 2009
10, 20 -- Received: from mailout10.t-online.de (mailout10.t-online.de [194.25.134.21])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAGHEAwj015853
10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2009 11:14:10 -0600
10, 20 -- Received: from fwd10.aul.t-online.de
10, 20 -- by mailout10.t-online.de with smtp
10, 20 -- id 1NA2T5-0007Hp-01; Mon, 16 Nov 2009 15:22:11 +0100
10, 20 -- Received: from [192.168.2.101] (X7gQZEZdohdIud-FtpoYISrvul9Glwu-UUXQSw03cUwMC24fI5jdX4AIGKntjMqQdR-at-[93.222.102.69]) by fwd10.aul.t-online.de
10, 20 -- with esmtp id 1NA2So-1Gsprk0; Mon, 16 Nov 2009 15:21:54 +0100
10, 20 -- Message-ID: {4B016002.6060505-at-t-online.de}
10, 20 -- Date: Mon, 16 Nov 2009 15:21:54 +0100
10, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
10, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
10, 20 -- MIME-Version: 1.0
10, 20 -- To: microscopy-at-microscopy.com
10, 20 -- Subject: FIB on bees
10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 20 -- Content-Transfer-Encoding: 7bit
10, 20 -- X-ID: X7gQZEZdohdIud-FtpoYISrvul9Glwu-UUXQSw03cUwMC24fI5jdX4AIGKntjMqQdR
10, 20 -- X-TOI-MSGID: 39043a49-1672-4a8a-8a3c-725fb4d341cb
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 16 Nov 2009 11:40:16 -0600
Subject: [Microscopy] Re: FIB on bees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan,

Fig. 07 looks like ion-milling artefact -- shows up in the other eye
images, also. You're in the cuticular lens of an ommatidium, which
(the lens) is on the order of 30 microns thick, and is a lamellar
structure (being as it is exoskeleton modified to be transparent).
I've seen this spikely structure in images of other FIB-milled
organics, like cells, but don't have any image references, sorry.

Phil

} Dear All,
} today I tried to mill into various parts of a honey-bee with not much
} success.
} Please have a look at these images:
} www.elektronenmikroskopie.info/beegroup/fib
} Specimen had been critical-point-dried and sputtered with ca. 10 to 15nm Pt.
} Milling had been with Gallium at 30KV and ca. 10 to 20 nA beam current.
}
} First three images are showing a cut of one of the claws, sorry for the
} missing close-up, which showed much curtaining. Depth is ca. 10um.
}
} Next images had been a trial run at the bee`s eye with various depth of
} milling: images 06 to 012 milled to a depth of ca. 1 um, images 13 to 16
} milled to 200 nm depth.
} I suppose, that original structure of the specimen is only to be seen at
} images 010, 015 and 16, just below the Pt-layer, but it may also be an
} artefact of redeposited Pt or Gallium.
}
} Any ideas if image 07 is showing artefacts of the milling or some
} structure of the bee-eye?
}
} Did anybody do FIB on organic material and is willing to send me thge
} procedure? Maybe a lower kv or current will give lesser artifacts?
}
} Best regards,
} Stefan
} --
} -----------------------------------------------------
} Stefan Diller - Scientifc Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49-931-7848700 Phone
} ++49-931-7848701 Fax
} ++49-175-7177051 Mobile
}
} Websites:
} www.stefan-diller.com
} www.elektronenmikroskopie.info
} www.assisi.de
} www.zwillingsprojekt.de
} Anfahrt: http://Mail.map24.com/Stefan.Diller
} -----------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 20 -- From stefan.diller-at-t-online.de Mon Nov 16 11:14:10 2009
} 10, 20 -- Received: from mailout10.t-online.de
} (mailout10.t-online.de [194.25.134.21])
} 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id nAGHEAwj015853
} 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2009
} 11:14:10 -0600
} 10, 20 -- Received: from fwd10.aul.t-online.de
} 10, 20 -- by mailout10.t-online.de with smtp
} 10, 20 -- id 1NA2T5-0007Hp-01; Mon, 16 Nov 2009 15:22:11 +0100
} 10, 20 -- Received: from [192.168.2.101]
} (X7gQZEZdohdIud-FtpoYISrvul9Glwu-UUXQSw03cUwMC24fI5jdX4AIGKntjMqQdR-at-[93.222.102.69])
} by fwd10.aul.t-online.de
} 10, 20 -- with esmtp id 1NA2So-1Gsprk0; Mon, 16 Nov 2009 15:21:54 +0100
} 10, 20 -- Message-ID: {4B016002.6060505-at-t-online.de}
} 10, 20 -- Date: Mon, 16 Nov 2009 15:21:54 +0100
} 10, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de}
} 10, 20 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
} 10, 20 -- MIME-Version: 1.0
} 10, 20 -- To: microscopy-at-microscopy.com
} 10, 20 -- Subject: FIB on bees
} 10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 10, 20 -- Content-Transfer-Encoding: 7bit
} 10, 20 -- X-ID:
} X7gQZEZdohdIud-FtpoYISrvul9Glwu-UUXQSw03cUwMC24fI5jdX4AIGKntjMqQdR
} 10, 20 -- X-TOI-MSGID: 39043a49-1672-4a8a-8a3c-725fb4d341cb
} ==============================End of - Headers==============================

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
5, 25 -- From oshel1pe-at-cmich.edu Mon Nov 16 11:40:15 2009
5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAGHeFlt031611
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Nov 2009 11:40:15 -0600
5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
5, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nAGHdb6n011470
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Nov 2009 12:40:14 -0500
5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
5, 25 -- Mon, 16 Nov 2009 12:40:11 -0500
5, 25 -- Mime-Version: 1.0
5, 25 -- Message-Id: {f0624080ec7273c0825ec-at-[141.209.160.249]}
5, 25 -- In-Reply-To: {200911161719.nAGHJgHJ021565-at-ns.microscopy.com}
5, 25 -- References: {200911161719.nAGHJgHJ021565-at-ns.microscopy.com}
5, 25 -- Date: Mon, 16 Nov 2009 12:40:09 -0500
5, 25 -- To: Microscopy-at-microscopy.com
5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 25 -- Subject: Re: [Microscopy] FIB on bees
5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
5, 25 -- X-OriginalArrivalTime: 16 Nov 2009 17:40:12.0067 (UTC) FILETIME=[D92C9F30:01CA66E3]
5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
5, 25 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
5, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
5, 25 -- X-CanItPRO-Stream: default
5, 25 -- X-Canit-Stats-ID: 22520012 - 66df4c762916 - 20091116
5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: sally.stowe-at-anu.edu.au
Date: Mon, 16 Nov 2009 14:39:28 -0600
Subject: [Microscopy] Re: FIB on bees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello  Stefan - I agree with Phil..the spikes are probably
re-deposited sputtered material from the specimen, and cutting down
the current would be a good first step.  A recent paper is:


J Microsc. 2009 Feb;233(2):309-19.
Comparison of different preparation methods of biological samples for
FIB milling and SEM investigation.

Leser V, Drobne D, Pipan Z, Milani M, Tatti F.

cheers

Sally Stowe



On 17/11/2009, oshel1pe-at-cmich.edu {oshel1pe-at-cmich.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Stefan,
}
} Fig. 07 looks like ion-milling artefact -- shows up in the other eye
} images, also. You're in the cuticular lens of an ommatidium, which
} (the lens) is on the order of 30 microns thick, and is a lamellar
} structure (being as it is exoskeleton modified to be transparent).
} I've seen this spikely structure in images of other FIB-milled
} organics, like cells, but don't have any image references, sorry.
}
} Phil
}
} } Dear All,
} } today I tried to mill into various parts of a honey-bee with not much
} } success.
} } Please have a look at these images:
} } www.elektronenmikroskopie.info/beegroup/fib
} } Specimen had been critical-point-dried and sputtered with ca. 10 to 15nm
} } Pt.
} } Milling had been with Gallium at 30KV and ca. 10 to 20 nA beam current.
} }
} } First three images are showing a cut of one of the claws, sorry for the
} } missing close-up, which showed much curtaining. Depth is ca. 10um.
} }
} } Next images had been a trial run at the bee`s eye with various depth of
} } milling: images 06 to 012 milled to a depth of ca. 1 um, images 13 to 16
} } milled to 200 nm depth.
} } I suppose, that original structure of the specimen is only to be seen at
} } images 010, 015 and 16, just below the Pt-layer, but it may also be an
} } artefact of redeposited Pt or Gallium.
} }
} } Any ideas if image 07 is showing artefacts of the milling or some
} } structure of the bee-eye?
} }
} } Did anybody do FIB on organic material and is willing to send me thge
} } procedure? Maybe a lower kv or current will give lesser artifacts?
} }
} } Best regards,
} } Stefan
} } --
} } -----------------------------------------------------
} } Stefan Diller - Scientifc Photography
} } Arndtstrasse 22
} } D - 97072 Wuerzburg Germany
} } ++49-931-7848700 Phone
} } ++49-931-7848701 Fax
} } ++49-175-7177051 Mobile
} }
} } Websites:
} } www.stefan-diller.com
} } www.elektronenmikroskopie.info


} } 10
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576
}
} ==============================Original Headers==============================


==============================Original Headers==============================
12, 40 -- From sallystowe-at-gmail.com Mon Nov 16 14:39:28 2009
12, 40 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAGKdRgq022099
12, 40 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2009 14:39:28 -0600
12, 40 -- Received: by pzk37 with SMTP id 37so3739224pzk.10
12, 40 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2009 12:39:27 -0800 (PST)
12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 40 -- d=gmail.com; s=gamma;
12, 40 -- h=domainkey-signature:mime-version:sender:reply-to:received
12, 40 -- :in-reply-to:references:from:date:x-google-sender-auth:message-id
12, 40 -- :subject:to:content-type:content-transfer-encoding;
12, 40 -- bh=kpWQwWRpI/nU7ibfiRBI7bYrIY6GkQpplvY3g79Nw7c=;
12, 40 -- b=L1dkl4+oFwc8LjCuDG9+Ww+FS9Y0HQ04qcRSn7ZwUvzEnCU/YthFgAjKhKQWL1UpVV
12, 40 -- HOX1G/99htPH2BkjQJaNkfPjH/TTbjEFch6MBV7MMuOy+SjgV7fnH5VmcSFfGwHaeDCw
12, 40 -- XPvhCmOVkXP364sk46/cJt24BKVdLKT0UnrQg=
12, 40 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
12, 40 -- d=gmail.com; s=gamma;
12, 40 -- h=mime-version:sender:reply-to:in-reply-to:references:from:date
12, 40 -- :x-google-sender-auth:message-id:subject:to:content-type
12, 40 -- :content-transfer-encoding;
12, 40 -- b=yB3RTQQLdvvEmOIjvFc1Mw6tCggvCGv2kabipM1RSqwzZRAplemByL7lX+FjQ12rd8
12, 40 -- THhu5dHddgX5hxfrYegK/ZLR9Uzdsq21s+5tAYaCMMF3odMgYPdESx890ncOFodySf+c
12, 40 -- mJ7RJZo0Kn2Jn3EWQmFRmsgskFuShqRwJtV8U=
12, 40 -- MIME-Version: 1.0
12, 40 -- Sender: sallystowe-at-gmail.com
12, 40 -- Reply-To: sally.stowe-at-anu.edu.au
12, 40 -- Received: by 10.142.74.8 with SMTP id w8mr931997wfa.192.1258403967330; Mon, 16
12, 40 -- Nov 2009 12:39:27 -0800 (PST)
12, 40 -- In-Reply-To: {ba4e3f220911161234l232b1733k811354d446f15744-at-mail.gmail.com}
12, 40 -- References: {200911161740.nAGHeM6B031745-at-ns.microscopy.com}
12, 40 -- {ba4e3f220911161234l232b1733k811354d446f15744-at-mail.gmail.com}
12, 40 -- From: Sally Stowe {sally.stowe-at-anu.edu.au}
12, 40 -- Date: Tue, 17 Nov 2009 07:39:05 +1100
12, 40 -- X-Google-Sender-Auth: fa3736943157acbf
12, 40 -- Message-ID: {ba4e3f220911161239j4a65ea4ep239397494fb03038-at-mail.gmail.com}
12, 40 -- Subject: Re: FIB on bees
12, 40 -- To: microscopy-at-microscopy.com
12, 40 -- Content-Type: text/plain; charset=ISO-8859-1
12, 40 -- Content-Transfer-Encoding: 8bit
12, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAGKdRgq022099
==============================End of - Headers==============================




From: kenikea-at-ornl.gov
Date: Tue, 17 Nov 2009 07:06:52 -0600
Subject: [Microscopy] SEM/FIB experience with LaB6 sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I received an email from an associate in Japan who wants to upgrade
the imaging performance of a W-gun FEI Quanta dual beam FIB with an
LaB6 source. Since I only have limited TEM experience with such
sources, I was wondering if there are strong proponents for one OEM
or another for SEM or FIB applications. Are the similar differences
in optimum operating conditions as for TEM? There may have been
previous discussions of performance and service life on this
listserver, but I am a newbie and unaware of them.
Thank you. ED kenik

Microscopy Group
Materials Science and Technology Division
Oak Ridge National Laboratory
100 Bethel Valley Rd., Bldg. 4515, MS-6064
PO Box 2008
Oak Ridge, TN 37831-6064, USA
Tel: 865 574-5066 Fax: 865 576-5413 e-mail: kenikea-at-ornl.gov


==============================Original Headers==============================
3, 23 -- From kenikea-at-ornl.gov Tue Nov 17 07:06:51 2009
3, 23 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAHD6puk029113
3, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Nov 2009 07:06:51 -0600
3, 23 -- Received: from emroute3.ornl.gov ([127.0.0.1])
3, 23 -- by emroute3.ornl.gov (PMDF V6.4 #31561)
3, 23 -- with ESMTP id {0KT9008G28FEXG-at-emroute3.ornl.gov} for
3, 23 -- Microscopy-at-Microscopy.Com; Tue, 17 Nov 2009 08:06:50 -0500 (EST)
3, 23 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov
3, 23 -- (PMDF V6.4 #31561) id {0KT9009018FEY8-at-emroute3.ornl.gov} for
3, 23 -- Microscopy-at-Microscopy.Com; Tue, 17 Nov 2009 08:06:50 -0500 (EST)
3, 23 -- Received: from kenikea-opt745.ornl.gov
3, 23 -- (kenikea-opt745.ornl.gov [160.91.156.84]) by emroute3.ornl.gov
3, 23 -- (PMDF V6.4 #31561) with ESMTP id {0KT9009GB8FE9C-at-emroute3.ornl.gov} for
3, 23 -- Microscopy-at-Microscopy.Com; Tue, 17 Nov 2009 08:06:50 -0500 (EST)
3, 23 -- Date: Tue, 17 Nov 2009 08:06:49 -0500
3, 23 -- From: Ed Kenik {kenikea-at-ornl.gov}
3, 23 -- Subject: SEM/FIB experience with LaB6 sources
3, 23 -- To: Microscopy-at-Microscopy.Com
3, 23 -- Message-id: {0KT9009GC8FE9C-at-emroute3.ornl.gov}
3, 23 -- MIME-version: 1.0
3, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
3, 23 -- Content-type: text/plain; format=flowed; charset=us-ascii
==============================End of - Headers==============================




From: bannigax-at-jmu.edu
Date: Tue, 17 Nov 2009 07:27:46 -0600
Subject: [Microscopy] TEM/SEM: spare biological samples?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,
I teach a light microscopy class for biology students which ends with
a quick look at electron microscopy. Since no one in my department
really uses the electron microscopes, we don't have access to much in
the way of biological specimens when demonstrating the TEM and SEM.
Does anyone have a few specimens that they are done with and would be
willing to donate to my class? They need not be great quality, just
something biological. You can contact me off-list at the email address
below.
Thanks in advance.
Alex

:::::::::::::::::::::::::::::::::::::::::::::
Alex Bannigan
Director of Microscopy
Biology Department, MSC 7801
James Madison University
Harrisonburg, VA, 22807, USA
bannigax-at-jmu.edu
phone: 540-5684521
http://csm.jmu.edu/biology/bannigax
::::::::::::::::::::::::::::::::::::::::::::

==============================Original Headers==============================
2, 33 -- From abannigan-at-gmail.com Tue Nov 17 07:27:46 2009
2, 33 -- Received: from mail-px0-f200.google.com (mail-px0-f200.google.com [209.85.216.200])
2, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAHDRkej012004
2, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Nov 2009 07:27:46 -0600
2, 33 -- Received: by pxi38 with SMTP id 38so1171504pxi.10
2, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Nov 2009 05:27:46 -0800 (PST)
2, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
2, 33 -- d=gmail.com; s=gamma;
2, 33 -- h=domainkey-signature:mime-version:sender:reply-to:received:date
2, 33 -- :x-google-sender-auth:message-id:subject:from:to:content-type;
2, 33 -- bh=4Qy65NWdL4CEnIq+48D8CtTEuUnyvW1Rzj+atO7FIsE=;
2, 33 -- b=l4KWVgh+fYIUOdep/VkUjgosqkZfSfub+n0EUWtNBumYRF4MaMdTE8D0dronu7WWho
2, 33 -- LE0pSqaddF9nXVYONz7PoXNplnMgWLH0UKD82k+24iQFFwn2NlOurUpKlAH8WjMk5ZuM
2, 33 -- rj/Sf2DyT0IQbfjNuhuFHPtXKcBPhhjSEvpKg=
2, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
2, 33 -- d=gmail.com; s=gamma;
2, 33 -- h=mime-version:sender:reply-to:date:x-google-sender-auth:message-id
2, 33 -- :subject:from:to:content-type;
2, 33 -- b=k3ePVrldlvpxD/rPh8yz1hiXafkyso4sXON60zGouqF6rcWp8QFjWnE8l2Ro4eLc+2
2, 33 -- p5dK7N6K3+sSj06dk4le0q7nieEl3w2JiMWg6kRb5bXjStbigksY44kV7Ca++M/7j+Px
2, 33 -- t+Wx3lgcol2/xG4biOMFMU7nVZLcHuG+xEMDM=
2, 33 -- MIME-Version: 1.0
2, 33 -- Sender: abannigan-at-gmail.com
2, 33 -- Reply-To: bannigax-at-jmu.edu
2, 33 -- Received: by 10.114.248.20 with SMTP id v20mr2213462wah.132.1258464465963;
2, 33 -- Tue, 17 Nov 2009 05:27:45 -0800 (PST)
2, 33 -- Date: Tue, 17 Nov 2009 08:27:45 -0500
2, 33 -- X-Google-Sender-Auth: f463996d0a151ed4
2, 33 -- Message-ID: {7f342c1c0911170527w26bb1d89vac75d452328a6a59-at-mail.gmail.com}
2, 33 -- Subject: TEM/SEM: spare biological samples?
2, 33 -- From: Alex Bannigan {bannigax-at-jmu.edu}
2, 33 -- To: Microscopy-at-microscopy.com
2, 33 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Tue, 17 Nov 2009 09:08:22 -0600
Subject: [Microscopy] Re: SEM/FIB experience with LaB6 sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ed,

In general, changing a W-source to a LaB6 source shouldn't be much of
an issue... providing the vacuum is acceptable. We've been using one
particular brand for quite a while and had good luck with
it. However, since I haven't tried other manufacturers recently, I
don't think I should make a recommendation of one manufacturer over another.

Peter Sewell at Kimball Physics has some useful information on LaB6
behavior in general, particularly in regards to residual vacuum. The
web page is
http://www.kimballphysics.com/cathode/cath_tech.htm

I will particularly point to technical bulletin #2 and the graph on
"surface recession vs. temperature". What these indicate is that the
partial pressure of oxygen (aka water vapor) in the cathode area is
critical. The evaporation rate of the oxide is over 3 orders of
magnitude higher than the boride. A vacuum system that is designed
for a W source may or may not be acceptable for LaB6. The mass loss
is only one of the problems when dealing with oxide formation. The
oxide is an insulator and when it deposits on the inside of the
wehnelt will result in an unstable beam.

In the Quanta, I believe the entire specimen chamber is vented for
sample exchange. Unless there is a gun isolation valve, this means
that the cathode will frequently be exposed to atmosphere. I
strongly suspect that the oxygen/water exposure on sample exchange
will severely impact cathode life.

Cheers,
Henk

At 08:08 AM 11/17/09, kenikea-at-ornl.gov wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


==============================Original Headers==============================
12, 26 -- From colijn.1-at-osu.edu Tue Nov 17 09:08:22 2009
12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAHF8MDu003758
12, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Nov 2009 09:08:22 -0600
12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
12, 26 -- id {01NG71KEA7LC95TLRF-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com;
12, 26 -- Tue, 17 Nov 2009 10:08:20 -0500 (EST)
12, 26 -- Received: from HOC1.ecr6.ohio-state.edu
12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu
12, 26 -- (PMDF V6.3-x18 #31556)
12, 26 -- with ESMTPA id {01NG71KBNVZ2962PTY-at-ecr6.ohio-state.edu} ; Tue,
12, 26 -- 17 Nov 2009 10:08:16 -0500 (EST)
12, 26 -- Date: Tue, 17 Nov 2009 09:53:05 -0500
12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
12, 26 -- Subject: Re: [Microscopy] SEM/FIB experience with LaB6 sources
12, 26 -- In-reply-to: {200911171308.nAHD8Gkg031231-at-ns.microscopy.com}
12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
12, 26 -- To: kenikea-at-ornl.gov
12, 26 -- Cc: Microscopy-at-microscopy.com
12, 26 -- Message-id: {01NG71KBQ174962PTY-at-ecr6.ohio-state.edu}
12, 26 -- MIME-version: 1.0
12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed
12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
12, 26 -- References: {200911171308.nAHD8Gkg031231-at-ns.microscopy.com}
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 17 Nov 2009 13:06:45 -0600
Subject: [Microscopy] Multi color stain for thick sections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

Does anyone out in thick sectioning of epon land have a simple one step stain that would sort of mimic an H&E?

I have to stain about 100 slides of serial thick sections through a zebra fish and want something simple, since I only have until before Nov. 26th to do this.

Thanks,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.





==============================Original Headers==============================
11, 20 -- From vapatpxs-at-yahoo.com Tue Nov 17 13:06:45 2009
11, 20 -- Received: from web46108.mail.sp1.yahoo.com (web46108.mail.sp1.yahoo.com [68.180.199.125])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAHJ6i7u012769
11, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Nov 2009 13:06:44 -0600
11, 20 -- Received: (qmail 37368 invoked by uid 60001); 17 Nov 2009 19:06:44 -0000
11, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1258484804; bh=uvyuy4wwhqDus8TGI6yzekBcXJIfKJ23S9CAXzPyDVE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=abjVLcbZiNlTjPDVUnVRnraXuxTNDO1mIumDASr/MOYFJJBA+IlFTEd2Lof3Gq5NvrNGG0jF9yuQWD1i02cvJgNxPA49O/0mvoCr+fOR3/37ZSaMQJQRfDUfDXw9/8lnrzrKLeKSpr0ofIDpb2N1Dz2SWUQLwVqF7RZoAoCfGU4=
11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
11, 20 -- s=s1024; d=yahoo.com;
11, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
11, 20 -- b=eu20FypK3wKZv8+nqGDcgOCawtmiXPsF39uYvOwmyI4rXu2ijAuLuFdaoWJhSkLlwcJ3J3s+4KvzvDe514bswRDijP2md3fDZSuMyOjewlcHIntsz7PT4gdOTLVzZZ5bQ/MTdMjKR1vwIu+YXHuxppa1HOsSSKnAW8KpP0eetUY=;
11, 20 -- Message-ID: {131169.36595.qm-at-web46108.mail.sp1.yahoo.com}
11, 20 -- X-YMail-OSG: k1WDZZoVM1nUdLkJZOgFhbqloyoQBlOu8osv8s8he65lOY3gZTidtpOipPsiPbwe3gdPO3tKkI4w3j4LHeAoO2vCKAAcVoFRoFzUTkpYqvRcI.8muBHaSHta4l0LXlwchxIroJLydrC2o6YkRNyiN0KsETV3U5IfsAulrpGnDKG3cg_BYogzwqtKhEnmc5gAE5QJ8yKw0akdcnflL7_9tW.NyFTAitzNZxg1At7dX7JFmXem3DBJSyF4z9MCGni0D9E-
11, 20 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Tue, 17 Nov 2009 11:06:44 PST
11, 20 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
11, 20 -- Date: Tue, 17 Nov 2009 11:06:44 -0800 (PST)
11, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
11, 20 -- Subject: Multi color stain for thick sections?
11, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
11, 20 -- MIME-Version: 1.0
11, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: jsiegmund-at-7thwavelabs.com
Date: Tue, 17 Nov 2009 14:43:57 -0600
Subject: [Microscopy] Multi color stain for thick sections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Multiple Stain Solution (Polysciences)works well.
To enhance the nuclei, use a Hematoxylin(Gills or Harris)step ahead of
the MMS stain.

Joachim

7thWaveLabs, St.Louis

-----Original Message-----
X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com]
Sent: Tuesday, November 17, 2009 1:17 PM
To: Joachim Siegmund

Hello Listers,

Does anyone out in thick sectioning of epon land have a simple one step
stain that would sort of mimic an H&E?

I have to stain about 100 slides of serial thick sections through a
zebra fish and want something simple, since I only have until before
Nov. 26th to do this.

Thanks,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.





==============================Original
Headers==============================
11, 20 -- From vapatpxs-at-yahoo.com Tue Nov 17 13:06:45 2009
11, 20 -- Received: from web46108.mail.sp1.yahoo.com
(web46108.mail.sp1.yahoo.com [68.180.199.125])
11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id nAHJ6i7u012769
11, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Nov 2009
13:06:44 -0600
11, 20 -- Received: (qmail 37368 invoked by uid 60001); 17 Nov 2009
19:06:44 -0000
11, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=yahoo.com; s=s1024; t=1258484804;
bh=uvyuy4wwhqDus8TGI6yzekBcXJIfKJ23S9CAXzPyDVE=;
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Ver
sion:Content-Type;
b=abjVLcbZiNlTjPDVUnVRnraXuxTNDO1mIumDASr/MOYFJJBA+IlFTEd2Lof3Gq5NvrNGG0
jF9yuQWD1i02cvJgNxPA49O/0mvoCr+fOR3/37ZSaMQJQRfDUfDXw9/8lnrzrKLeKSpr0ofI
Dpb2N1Dz2SWUQLwVqF7RZoAoCfGU4=
11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
11, 20 -- s=s1024; d=yahoo.com;
11, 20 --
h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Ver
sion:Content-Type;
11, 20 --
b=eu20FypK3wKZv8+nqGDcgOCawtmiXPsF39uYvOwmyI4rXu2ijAuLuFdaoWJhSkLlwcJ3J3
s+4KvzvDe514bswRDijP2md3fDZSuMyOjewlcHIntsz7PT4gdOTLVzZZ5bQ/MTdMjKR1vwIu
+YXHuxppa1HOsSSKnAW8KpP0eetUY=;
11, 20 -- Message-ID: {131169.36595.qm-at-web46108.mail.sp1.yahoo.com}
11, 20 -- X-YMail-OSG:
k1WDZZoVM1nUdLkJZOgFhbqloyoQBlOu8osv8s8he65lOY3gZTidtpOipPsiPbwe3gdPO3tK
kI4w3j4LHeAoO2vCKAAcVoFRoFzUTkpYqvRcI.8muBHaSHta4l0LXlwchxIroJLydrC2o6Yk
RNyiN0KsETV3U5IfsAulrpGnDKG3cg_BYogzwqtKhEnmc5gAE5QJ8yKw0akdcnflL7_9tW.N
yFTAitzNZxg1At7dX7JFmXem3DBJSyF4z9MCGni0D9E-
11, 20 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com
via HTTP; Tue, 17 Nov 2009 11:06:44 PST
11, 20 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
11, 20 -- Date: Tue, 17 Nov 2009 11:06:44 -0800 (PST)
11, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
11, 20 -- Subject: Multi color stain for thick sections?
11, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
11, 20 -- MIME-Version: 1.0
11, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of -
Headers==============================

This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer


==============================Original Headers==============================
21, 30 -- From jsiegmund-at-7thwavelabs.com Tue Nov 17 14:43:56 2009
21, 30 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136])
21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAHKhu3A031272
21, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Nov 2009 14:43:56 -0600
21, 30 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1])
21, 30 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 57DAE398015;
21, 30 -- Tue, 17 Nov 2009 14:43:49 -0600 (CST)
21, 30 -- X-AuditID: c0a80218-ad57abb000000bc3-35-4b030afc595d
21, 30 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29])
21, 30 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 6922D424016;
21, 30 -- Tue, 17 Nov 2009 14:43:40 -0600 (CST)
21, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
21, 30 -- Content-class: urn:content-classes:message
21, 30 -- MIME-Version: 1.0
21, 30 -- Content-Type: text/plain;
21, 30 -- charset="us-ascii"
21, 30 -- Subject: RE: [Microscopy] Multi color stain for thick sections?
21, 30 -- Date: Tue, 17 Nov 2009 14:43:39 -0600
21, 30 -- Message-ID: {62A8156F8071C8439080D626DF8C33A6BAC3D2-at-wave-mail.7thwave.local}
21, 30 -- X-MS-Has-Attach:
21, 30 -- X-MS-TNEF-Correlator:
21, 30 -- Thread-Topic: [Microscopy] Multi color stain for thick sections?
21, 30 -- Thread-Index: AcpnwRBTPvEj5slFS+qL1Y0KH2wfbwABDVMg
21, 30 -- References: {200911171916.nAHJGtMK025270-at-ns.microscopy.com}
21, 30 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com}
21, 30 -- To: {vapatpxs-at-yahoo.com}
21, 30 -- Cc: {Microscopy-at-Microscopy.Com}
21, 30 -- X-Brightmail-Tracker: AAAAARGvZsI=
21, 30 -- Content-Transfer-Encoding: 8bit
21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAHKhu3A031272
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Tue, 17 Nov 2009 15:12:26 -0600
Subject: [Microscopy] Paragon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everybody,

I have received lots of suggestions, one of which is Paragon. I tried Paragon and it did make a lovely pink and purple stain, it also stain the naked epon purple. I tried ethanol washing after the stain but the epon stayed purple.

Any suggestions as to how get rid of my Purple Haze?

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.





==============================Original Headers==============================
10, 20 -- From vapatpxs-at-yahoo.com Tue Nov 17 15:12:26 2009
10, 20 -- Received: from web46108.mail.sp1.yahoo.com (web46108.mail.sp1.yahoo.com [68.180.199.125])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAHLCQxr014305
10, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Nov 2009 15:12:26 -0600
10, 20 -- Received: (qmail 14240 invoked by uid 60001); 17 Nov 2009 21:12:25 -0000
10, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1258492345; bh=GL93e9qH4wPUxW09YfVtuAKCJg0NOmC2ObgQkSW4JtA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=ADK3E1ySoY52907lW2xgQFDmp3hhh9gNo+yo/mltMVUmNU/Ei+0JyjG19lhm3NQ0psDH7hKQ4j6Rlq4RCFj9WD2j4RiIl/n0/bUOylzJAo7CXrcKVP2xdT4whYvfC+g64UGFGIB6LEFb+oZNokLk6/JHwlly1L1cbuEd7R74VqE=
10, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 20 -- s=s1024; d=yahoo.com;
10, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
10, 20 -- b=kf6FXd9PTM6RO7QoQx30Po7ainIOpppY94oWq4sIHEbTJZ1BAK8R+SnJE14A/6UKzSZrbTx4I73sSjQ1hk4hK+m23GCPECpErwaEp9q3OhkY3RwH9JZIKW2RX4z1nrOur01reCUAJ0ewT8RqWXnk7rnFkj6e68j/VadP/TW+EW0=;
10, 20 -- Message-ID: {680518.12778.qm-at-web46108.mail.sp1.yahoo.com}
10, 20 -- X-YMail-OSG: I8FXq9QVM1mGF93EhtI9hazwk3wPHMAJsPYgH0WbiKevuoAyKLJpEn8Nc7maikX180K4k1gPkaYnpxv.avK2iWMVDpjOlnPY1KME8ukiFAFCaVQGQrETIJGCQzG1AmMPaGXWYY_NXJaQLU1cKGzVi6b78WNy09nPhadZc5RrR.l1DUxg4myPvSEOCHspaTlyMZ6HVI8dice.2mvcuUsiBRbzuRlY1FiJdCw400l5RJud1h32.dM3wrLggwYa_4.Nej0-
10, 20 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Tue, 17 Nov 2009 13:12:25 PST
10, 20 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.7.361.4
10, 20 -- Date: Tue, 17 Nov 2009 13:12:25 -0800 (PST)
10, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
10, 20 -- Subject: Paragon
10, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
10, 20 -- MIME-Version: 1.0
10, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: bloos-at-sun.ac.za
Date: Tue, 17 Nov 2009 16:37:23 -0600
Subject: [Microscopy] viaWWW: Acridine Orange

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bloos-at-sun.ac.za as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bloos-at-sun.ac.za
Name: Ben Loos

Organization: Stellenbosch University

Title-Subject: [Filtered] Acridine Orange

Question: Dear Colleges,

I would like to ask about the exact binding mechanism of the dye
acridine orange. It is able to intercalate with DNA and also
accumulates in acidic compartments? what is the mechanism behind that?

It is confusing, as this dye seems to be very versatile, used for
apoptosis/nuclear condensation, but also for autophagy/lyosomal stain.

could someone please clarify that for me, from the modes of action of this dye?

thank you very much, your advise is much appreciated.
best wishes
Ben

Ben Loos
Central Analytical Facility
Stellenbosch University
South Africa



Login Host: 146.232.65.6
---------------------------------------------------------------------------

==============================Original Headers==============================
13, 11 -- From zaluzec-at-microscopy.com Tue Nov 17 16:37:21 2009
13, 11 -- Received: from [10.158.144.171] (91-103-36-171.dynamic.thecloud.net [91.103.36.171])
13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAHMbJgN031199
13, 11 -- for {microscopy-at-microscopy.com} ; Tue, 17 Nov 2009 16:37:20 -0600
13, 11 -- Mime-Version: 1.0
13, 11 -- Message-Id: {p06240800c72807e1bdc4-at-[10.158.144.171]}
13, 11 -- Date: Tue, 17 Nov 2009 08:57:40 +0100
13, 11 -- To: microscopy-at-microscopy.com
13, 11 -- From: bloos-at-sun.ac.za (by way of MicroscopyListserver)
13, 11 -- Subject: viaWWW: Acridine Orange
13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Karsten.Goemann-at-utas.edu.au
Date: Wed, 18 Nov 2009 00:11:18 -0600
Subject: [Microscopy] Re: SEM/FIB experience with LaB6 sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We've got a tungsten FEI Quanta 600 MkI (2005 vintage), which only
has one turbo pump for the whole instrument (i.e. no additional high
vacuum pump(s) in the gun area) and no gun isolation valve (i.e. the
whole instrument is vented for a sample exchange).
Although that system pumps down to something like 6E-7 torr over
night if left alone, I suspect LaB6 on such a system is probably not
going to work.

I used LaB6 cathodes on our electron microprobe and was advised to
always keep it hot (never switch the heat off) for best performance
and lifetime. That instrument also has an ion getter pump as third
pumping stage in the gun area. I was told that exposure to air while
it is still at elevated temperatures will lead to oxidation on the
tip which destroys it.

Cheers,

Karsten

----
Dr. Karsten Goemann
Scientific Officer-in-Charge, Electron Microscopy & X-ray Microanalysis
Central Science Laboratory, University of Tasmania
Private Bag 74, Hobart TAS 7001, Australia
Tel.: +61 (0) 3 6226 2146
Fax: +61 (0) 3 6226 2494
E-mail: Karsten.Goemann-at-utas.edu.au
www.utas.edu.au/csl


} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
8, 22 -- From Karsten.Goemann-at-utas.edu.au Wed Nov 18 00:11:17 2009
8, 22 -- Received: from corinna.its.utas.edu.au (corinna.its.utas.edu.au [131.217.10.51])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAI6BGvG030151
8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 00:11:17 -0600
8, 22 -- Received: from mmp-sby1.its.utas.edu.au (mmp-sby1.its.utas.edu.au [10.10.3.71])
8, 22 -- by corinna.its.utas.edu.au (8.13.8+Sun/8.13.6) with ESMTP id nAI6BC3F029378
8, 22 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 17:11:13 +1100 (EST)
8, 22 -- MIME-version: 1.0
8, 22 -- Content-transfer-encoding: 7BIT
8, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed
8, 22 -- Received: from [131.217.50.60] ([unknown] [131.217.50.60])
8, 22 -- by mmp-sby1.its.utas.edu.au
8, 22 -- (Sun Java(tm) System Messaging Server 7.0-3.01 64bit (built Dec 9 2008))
8, 22 -- with ESMTP id {0KTA0078XJUOY310-at-mmp-sby1.its.utas.edu.au} for
8, 22 -- microscopy-at-microscopy.com; Wed, 18 Nov 2009 17:11:12 +1100 (EST)
8, 22 -- Message-id: {a06240805c7293b46b259-at-[131.217.50.60]}
8, 22 -- In-reply-to: {200911171513.nAHFDWaX012005-at-ns.microscopy.com}
8, 22 -- References: {200911171513.nAHFDWaX012005-at-ns.microscopy.com}
8, 22 -- Date: Wed, 18 Nov 2009 17:11:24 +1100
8, 22 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
8, 22 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au}
8, 22 -- Subject: [Microscopy] Re: SEM/FIB experience with LaB6 sources
==============================End of - Headers==============================




From: ahmad_ds-at-yahoo.com
Date: Wed, 18 Nov 2009 08:59:18 -0600
Subject: [Microscopy] viaWWW: TEM Sample Preparation Centre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] TEM Sample Preparation Centre

Question: Dear all,

Could anybody please tell me where you can get a very good training
on sample preparation for different types of materials for TEM?

Thank you very much.



Login Host: 87.236.233.99
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Nov 18 08:59:18 2009
10, 11 -- Received: from [129.16.193.210] (dhcp-193-210.nomad.chalmers.se [129.16.193.210])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIExHnp006775
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 08:59:18 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c729bb450747-at-[10.158.144.171]}
10, 11 -- Date: Wed, 18 Nov 2009 15:55:17 +0100
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TEM Sample Preparation Centre
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ahmad_ds-at-yahoo.com
Date: Wed, 18 Nov 2009 08:59:21 -0600
Subject: [Microscopy] viaWWW: TEM Sample Preparation Centre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] TEM Sample Preparation Centre

Question: Dear all,

Could anybody please tell me where you can get a very good training
on sample preparation for different types of materials for TEM?

Thank you very much.



Login Host: 87.236.233.99
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Nov 18 08:59:20 2009
10, 11 -- Received: from [129.16.193.210] (dhcp-193-210.nomad.chalmers.se [129.16.193.210])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIExHnr006775
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 08:59:19 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c729bb9c1bbe-at-[78.64.120.92]}
10, 11 -- Date: Wed, 18 Nov 2009 15:56:30 +0100
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: TEM Sample Preparation Centre
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rajeevrajvihar-at-gmail.com
Date: Wed, 18 Nov 2009 09:00:38 -0600
Subject: [Microscopy] viaWWW: Field Emission or Thermionic Emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rajeevrajvihar-at-gmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rajeevrajvihar-at-gmail.com
Name: Rajeev

Organization: Indian Space Research Organization

Title-Subject: [Filtered] Field Emission or Thermionic Emission

Question: Hi,

We are in the process of buying a TEM. While making the
specifications, there was a serious debate on whether we should go
for a field emission TEM or conventional TEM (tungsten filament). The
argument against FEG is that maintaining a field emission TEM is
difficult as we have power outage on and off and also the lab working
hours are 9-5. Therefore if power outage is there in the nighttime,
there is no alternative for power supply and this may damage the
machine. Also, it will take a lot of time for the FEG to get ready
for analysis compared to conventional TEM. However, since a part of
our samples are polymers and polymer nanocomposite, in order to have
very good resolution and current density, we think we have to go for
FEG. Therefore, my doubts are:

1. Is it true that field emission TEM should run continuously 24x7
with uninterrupted power supply?

2. Is there any problem for the gun or TEM if power goes off in
between? Will it affect the life and performance of the gun and
machine?

3. Will it take a lot of time for field emission TEM for start up and
ready for analysis?

4. For the analysis of polymer nanocomposites (matrix filled with
nanoclays, carbon nanotubes etc) and nanomaterials, do we really need
a field emission TEM?

5. Altogether, is the management of field emission TEM is difficult
compared to conventional TEM?

I am sorry for the long list of questions; however, I would be
extermely thankful if my doubts on FEG and conventional TEM are
clarified.

Thanks in advance,

Dr. R.S. Rajeev
Scientist/Engineer
Polymers and Special Chemicals Division
Vikram Sarabhai Space Centre
Indian Space Research Organization
Trivandrum
Kerala, India.

Login Host: 117.199.9.236
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Wed Nov 18 09:00:38 2009
15, 11 -- Received: from [129.16.193.210] (dhcp-193-210.nomad.chalmers.se [129.16.193.210])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIF0Zvq009325
15, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 09:00:37 -0600
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240802c729bc5747a0-at-[129.16.193.210]}
15, 11 -- Date: Wed, 18 Nov 2009 16:00:34 +0100
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: rajeevrajvihar-at-gmail.com (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Field Emission or Thermionic Emission
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 18 Nov 2009 11:35:40 -0600
Subject: [Microscopy] EDS in TEM - SiLi and SDD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List,

We're writing a grant for a new TEM which will include EDS to be used
for life sciences and materials sciences. I'm more used to SiLi
detectors on SEMs, so I would like some input from the materials
folks on what to look for in EDS detectors on TEMs, and the pros and
cons of SiLi vs. SDD. I've got all the usual references, so I'm
looking for user experiences.
Vendor responses off-line welcome.
Note: TEM location may well limit us to 120kV - 200kV would be
better, yes, but likely not doable.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
3, 23 -- From oshel1pe-at-cmich.edu Wed Nov 18 11:35:40 2009
3, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIHZe0l026036
3, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 11:35:40 -0600
3, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74])
3, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nAIHZX2O001895
3, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:35:39 -0500
3, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
3, 23 -- Wed, 18 Nov 2009 12:35:38 -0500
3, 23 -- Mime-Version: 1.0
3, 23 -- Message-Id: {f06240809c729df17d51b-at-[141.209.160.249]}
3, 23 -- Date: Wed, 18 Nov 2009 12:35:34 -0500
3, 23 -- To: Microscopy-at-microscopy.com
3, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 23 -- Subject: EDS in TEM - SiLi and SDD detectors
3, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 23 -- X-OriginalArrivalTime: 18 Nov 2009 17:35:39.0394 (UTC) FILETIME=[8B798620:01CA6875]
3, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 23 -- X-Spam-Score: -4.40 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
3, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
3, 23 -- X-CanItPRO-Stream: default
3, 23 -- X-Canit-Stats-ID: 22698694 - 65024aaffa7a - 20091118
3, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: richard.ross-at-allisontransmission.com
Date: Wed, 18 Nov 2009 12:04:39 -0600
Subject: [Microscopy] EDS in TEM - SiLi and SDD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Phil,
Referring to the article by Dale Newbury, NIST, referenced below, there is
only one major parameter where a SiLi detector outperforms an SDD
detector: the capture efficiency for photons above 10 KeV.
http://spectroscopyonline.findanalytichem.com/spectroscopy/Featured+Flash+Component/The-Revolution-in-Energy-Dispersive-X-Ray-Spectrom/ArticleStandard/Article/detail/609461

Rick Ross
Sr. Metallurgist
Allison Transmission, Inc.
Indianapolis, IN, USA


==============================Original Headers==============================
3, 25 -- From richard.ross-at-allisontransmission.com Wed Nov 18 12:04:38 2009
3, 25 -- Received: from plmler11.mail.eds.com (plmler11.mail.eds.com [199.228.142.91])
3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAII4bLb009755
3, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:38 -0600
3, 25 -- Received: from plmlir5.mail.eds.com (plmlir5-2.mail.eds.com [199.228.142.135])
3, 25 -- by plmler11.mail.eds.com (8.14.2/8.13.8) with ESMTP id nAII4bmB019828
3, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:37 -0600
3, 25 -- Received: from plmlir5.mail.eds.com (localhost [127.0.0.1])
3, 25 -- by plmlir5.mail.eds.com (8.14.2/8.12.10) with ESMTP id nAII4Rk5024742
3, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:27 -0600
3, 25 -- Received: from USAT0EM0AD01.MAIL.ATI.INT (USAT0EM0AD01.us.ad.ati.int [143.242.253.24])
3, 25 -- by plmlir5.mail.eds.com (8.14.2/8.12.10) with ESMTP id nAII4RPM024729
3, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:27 -0600
3, 25 -- X-EDSINT-Source-Ip: 143.242.253.24
3, 25 -- To: microscopy-at-microscopy.com
3, 25 -- Subject: [Microscopy] EDS in TEM - SiLi and SDD detectors
3, 25 -- MIME-Version: 1.0
3, 25 -- X-Mailer: Lotus Notes Release 6.5.4 HF1151 March 27, 2007
3, 25 -- Message-ID: {OF57090C14.5689DAA6-ON85257672.0062A63C-85257672.00634898-at-mail.ati.int}
3, 25 -- From: richard.ross-at-allisontransmission.com
3, 25 -- Date: Wed, 18 Nov 2009 13:04:24 -0500
3, 25 -- X-MIMETrack: Serialize by Router on USAT0EM0AD01/ADMINHG/ATISERVER/ATI(Release 8.0.1|February
3, 25 -- 07, 2008) at 11/18/2009 01:04:27 PM,
3, 25 -- Serialize complete at 11/18/2009 01:04:27 PM
3, 25 -- Content-Type: text/plain; charset="US-ASCII"
==============================End of - Headers==============================




From: kpruessner-at-gmail.com
Date: Wed, 18 Nov 2009 13:35:26 -0600
Subject: [Microscopy] Hitachi Akashi MSM-4 tabletop SEM, manuals and diagrams wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I have inherited an old table-top SEM Hitachi Akashi, model MSM-4,
apparently built in 1974. At the moment the HV doesn't work. Would
anyone have a manual and circuit diagrams for this machine? How about
parts for the machine or another machine like this that could be used
for parts? We would like to try and bring it back to life for demo
purposes.

Best regards

Karin

==============================Original Headers==============================
4, 30 -- From kpruessner-at-gmail.com Wed Nov 18 13:35:26 2009
4, 30 -- Received: from mail-fx0-f217.google.com (mail-fx0-f217.google.com [209.85.220.217])
4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIJZPa5029121
4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 13:35:26 -0600
4, 30 -- Received: by fxm9 with SMTP id 9so1672539fxm.10
4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 11:35:25 -0800 (PST)
4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 30 -- d=gmail.com; s=gamma;
4, 30 -- h=domainkey-signature:mime-version:received:reply-to:date:message-id
4, 30 -- :subject:from:to:content-type;
4, 30 -- bh=waENl8cg/laAzegPez1c395Izmj/Ho1uhme00xxs4sE=;
4, 30 -- b=wW26zX/UUipd1RJEKLc1DkleS0q1ZnCgY95CKkz/tSP6NcPvj9jNf6MRVytqjPSXzZ
4, 30 -- 3r+MHqQMVsUen5kKEPMo4J+CwVZP6yZm8BPXlxt3+cijYQPVOza+5jq4HkndaPKfx5K3
4, 30 -- siubjdZJw/iJiwVVgWgiFCZv4xdMxdO+Tf24c=
4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
4, 30 -- d=gmail.com; s=gamma;
4, 30 -- h=mime-version:reply-to:date:message-id:subject:from:to:content-type;
4, 30 -- b=hkeZKTiiC0SjqX22bk1l1l7lRS4eu1FyuUgnEb5W8OxcJqOezaGCLjlAUsH4nbmXk9
4, 30 -- LVT89yMTHnU2wR9OEEOSBpVA3D0ExDjcvXTHsNVSWM536V5ALMnMIQOm9laDRUco29MA
4, 30 -- kvdHdrHWty8/PsBRg1WPj9UByTJ2pqrM87cgI=
4, 30 -- MIME-Version: 1.0
4, 30 -- Received: by 10.223.17.203 with SMTP id t11mr1037427faa.75.1258572925425; Wed,
4, 30 -- 18 Nov 2009 11:35:25 -0800 (PST)
4, 30 -- Reply-To: kpruessner-at-gmail.com
4, 30 -- Date: Wed, 18 Nov 2009 21:35:25 +0200
4, 30 -- Message-ID: {90f8aecf0911181135r249cee9dr5fe3c8f5dcbb9761-at-mail.gmail.com}
4, 30 -- Subject: Hitachi Akashi MSM-4 tabletop SEM, manuals and diagrams wanted
4, 30 -- From: Karin Pruessner {kpruessner-at-gmail.com}
4, 30 -- To: Microscopy-at-microscopy.com
4, 30 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Wed, 18 Nov 2009 14:12:32 -0600
Subject: [Microscopy] LEICA S440 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,
Anyone out there that would have an old manufacturers sales/data
sheet for this particular SEM? I would appreciate if it could be
scanned and emailed to me. If anyone has a parts only S440 SEM out
there I would be very interested in purchasing the main console cover
panels. Please reply directly to my email, Thanks in advance.

Gary Easton, Pres.
SCANNERS CORPORATION
SEM Service
410-857-7633


==============================Original Headers==============================
3, 20 -- From garyeaston-at-scannerscorp.com Wed Nov 18 14:12:31 2009
3, 20 -- Received: from omr10.networksolutionsemail.com (omr10.networksolutionsemail.com [205.178.146.60])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIKCVm8013197
3, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 14:12:31 -0600
3, 20 -- Received: from localhcloudmarkgw1 (mail.networksolutionsemail.com [205.178.146.50])
3, 20 -- by omr10.networksolutionsemail.com (8.13.6/8.13.6) with ESMTP id nAIKCV8w022957
3, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 15:12:31 -0500
3, 20 -- Received: from [72.81.190.77] ([72.81.190.77:61343] helo=[192.168.1.3])
3, 20 -- by localhcloudmarkgw1 (envelope-from {garyeaston-at-scannerscorp.com} )
3, 20 -- (ecelerity 2.2.2.41 r(31179/31189)) with ESMTP
3, 20 -- id 92/F2-06996-6B4540B4; Wed, 18 Nov 2009 15:10:31 -0500
3, 20 -- Message-ID: {4B045532.2070205-at-scannerscorp.com}
3, 20 -- Date: Wed, 18 Nov 2009 15:12:34 -0500
3, 20 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 20 -- MIME-Version: 1.0
3, 20 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 20 -- Subject: LEICA S440 SEM
3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 18 Nov 2009 14:33:57 -0600
Subject: [Microscopy] LM Olympus BHTU (BH2) PC bd.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
I received this via snail mail a few weeks ago and thought that it might be
helpful to some LM users.
******************************************************************

J.C. Ritchey Co.
Microscope Service
7485 Lancaster-Newark Rd. NE
Baltimore, OH 43105
740-862-9252
Fax: 740-862-9134

October 26,2009

Dear Sir,
My name is James C. Ritchey and I have worked and owned my own microscope
service business for over the past 30 years.

It has come to my attention that Olympus, (microscope manufacturer) has quit
supplying the PC Boards for the model BHTU (BH2) microscope. The reason
being is that, some of the components on the board are no longer being
manufactured.

I, however, have employed an electronic engineer that has redesigned the PC
Board for me. I have used these PC Boards in many Olympus microscopes and
they have performed perfectly.

I am writing to you, to inform you that these PC Boards are now available
through my company under the order number of #JC100 at the cost of $100.00
each. I also have available all other electronic parts for the Olympus BHTU
microscope.

Please feel free to call me if you wish to order these PC Boards at the
number listed above, or if you have any questions at all. Thank you.

Sincerely,
James C. Ritchey
************************************************************

Hopefully it might be a help to someone.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz






==============================Original Headers==============================
17, 24 -- From kenconverse-at-qualityimages.biz Wed Nov 18 14:33:56 2009
17, 24 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAIKXtc7028437
17, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 14:33:56 -0600
17, 24 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com
17, 24 -- with ESMTP
17, 24 -- id {20091118203354381.BTAB27026-at-cdptpa-omta02.mail.rr.com}
17, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 20:33:54 +0000
17, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
17, 24 -- To: "MSA Listserver" {microscopy-at-microscopy.com}
17, 24 -- Subject: LM Olympus BHTU (BH2) PC bd.
17, 24 -- Date: Wed, 18 Nov 2009 15:33:46 -0500
17, 24 -- Message-ID: {CA3315AE382A4B9BBDA04B9C0E6516D8-at-Ken}
17, 24 -- MIME-Version: 1.0
17, 24 -- Content-Type: text/plain;
17, 24 -- charset="us-ascii"
17, 24 -- X-Priority: 3 (Normal)
17, 24 -- X-MSMail-Priority: Normal
17, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
17, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
17, 24 -- Importance: Normal
17, 24 -- Thread-Index: Acpojm1wdJGr+KkvRmOpZGR2KjP3tA==
17, 24 -- Content-Transfer-Encoding: 8bit
17, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAIKXtc7028437
==============================End of - Headers==============================




From: l-reyjr-at-northwestern.edu
Date: Wed, 18 Nov 2009 15:21:34 -0600
Subject: [Microscopy] viaWWW: Prevent block face from Concaving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both l-reyjr-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: Northwestern University

Title-Subject: [Filtered] Prevent block face from Concaving

Question: I need your help with this problem. When embedding cell
cultures in LR White or Embed812 and Araldite mixture on coverslips
(glass or thermanox). When I invert beem capsule or gelatin capsule
onto coverslip. Then place block in 60 degree oven. The block surface
becomes concave. Is there anyway to prevent this. When I thin
section, the sectioning begins at the four corners of the block face.

Thanks in Advance
Lennell

Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Nov 18 15:21:34 2009
7, 11 -- Received: from [129.16.193.210] (91-103-36-171.dynamic.thecloud.net [91.103.36.171])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAILLWwn012317
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 15:21:33 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240808c72a15c05e97-at-[129.16.193.210]}
7, 11 -- Date: Wed, 18 Nov 2009 22:21:31 +0100
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Prevent block face from Concaving
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: fahayes-at-ucdavis.edu
Date: Wed, 18 Nov 2009 18:45:55 -0600
Subject: [Microscopy] need suggestions for replacing /upgrading the XL30 SEM pc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone out there have any suggestions regarding replacing/upgrading
the pc for the XL30 SFEG SEM? Please respond offline

Thank you

--
Fred A. Hayes
Manager, Central Facilities
Department of Chemical Engineering and Material Sciences
3118 Bainer Hall
Bainer Hall Drive
UC Davis
Davis, CA 95616
530-752-0284 office
530-754-6350 fax
707-761-9045 cell
fahayes-at-ucdavis.edu
http://www.matscicf.ucdavis.edu/









==============================Original Headers==============================
11, 19 -- From fahayes-at-ucdavis.edu Wed Nov 18 18:45:55 2009
11, 19 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJ0js4f001915
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Nov 2009 18:45:55 -0600
11, 19 -- Received: from [169.237.230.148] ([169.237.230.148])
11, 19 -- by smtp3.ucdavis.edu (8.13.1/8.13.1/it-cyrus-5.2.0) with ESMTP id nAJ0jrfB061842
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Nov 2009 16:45:53 -0800
11, 19 -- Message-ID: {4B049543.1020208-at-ucdavis.edu}
11, 19 -- Date: Wed, 18 Nov 2009 16:45:55 -0800
11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu}
11, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
11, 19 -- MIME-Version: 1.0
11, 19 -- To: Microscopy-at-Microscopy.Com
11, 19 -- Subject: need suggestions for replacing /upgrading the XL30 SEM pc
11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- X-Virus-Scanned: clamav-milter 0.95.1 at av8
11, 19 -- X-Virus-Status: Clean
11, 19 -- X-Scanned-By: MIMEDefang 2.67 on 128.120.32.8
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Wed, 18 Nov 2009 21:42:02 -0600
Subject: [Microscopy] CryoTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I am looking for someone who is interested in working with a company
research group to do cryoTEM of liposomes. I think the project is quite
limited in scope and would need to be done in the near future.

Please contact me off-line if you are interested in pursuing this and I will
forward your contact information to my contact.

Thanks,
Debby


---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy




==============================Original Headers==============================
9, 31 -- From dsherman-at-purdue.edu Wed Nov 18 21:42:01 2009
9, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJ3g1fc023384
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 21:42:01 -0600
9, 31 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127])
9, 31 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id nAJ3g1ru007168
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 22:42:01 -0500
9, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93])
9, 31 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id nAJ3g1H0013985
9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 22:42:01 -0500
9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.213]) by WPPEXHUB04H.purdue.lcl
9, 31 -- ([::1]) with mapi; Wed, 18 Nov 2009 22:42:01 -0500
9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
9, 31 -- Date: Wed, 18 Nov 2009 22:41:58 -0500
9, 31 -- Subject: CryoTEM
9, 31 -- Thread-Topic: CryoTEM
9, 31 -- Thread-Index: Acpoyj7QkVmAIsUaQU6kuSU5NHyrBg==
9, 31 -- Message-ID: {C72A28B6.22B8%dsherman-at-purdue.edu}
9, 31 -- Accept-Language: en-US
9, 31 -- Content-Language: en-US
9, 31 -- X-MS-Has-Attach:
9, 31 -- X-MS-TNEF-Correlator:
9, 31 -- user-agent: Microsoft-Entourage/13.0.0.090609
9, 31 -- acceptlanguage: en-US
9, 31 -- Content-Type: text/plain; charset="us-ascii"
9, 31 -- MIME-Version: 1.0
9, 31 -- X-PMX-Version: 5.5.7.378829
9, 31 -- X-PerlMx-Virus-Scanned: Yes
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJ3g1fc023384
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 19 Nov 2009 07:18:35 -0600
Subject: [Microscopy] EDS in TEM - SiLi and SDD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This concerns SEM not TEM, which was the point of Philip's message.
As I understood it, the real advantage of SiLi detectors are in high count rates, which specifically never happen in TEM. Actually sensitivity is a major point for EDS analysis in TEM.
I would tend to think that SiLi detectors offer no real advantage in these conditions, but I would be grateful if everyone would care to share their comments on this question with the community.

regards,

Stephane

 


----- Original Message ----
X-from: "richard.ross-at-allisontransmission.com" {richard.ross-at-allisontransmission.com}
To: nizets2-at-yahoo.com
Sent: Wed, November 18, 2009 7:08:36 PM

Phil,
Referring to the article by Dale Newbury, NIST, referenced below, there is
only one major parameter where a SiLi detector outperforms an SDD
detector: the capture efficiency for photons above 10 KeV.
http://spectroscopyonline.findanalytichem.com/spectroscopy/Featured+Flash+Component/The-Revolution-in-Energy-Dispersive-X-Ray-Spectrom/ArticleStandard/Article/detail/609461

Rick Ross
Sr. Metallurgist
Allison Transmission, Inc.
Indianapolis, IN, USA


==============================Original Headers==============================
3, 25 -- From richard.ross-at-allisontransmission.com Wed Nov 18 12:04:38 2009
3, 25 -- Received: from plmler11.mail.eds.com (plmler11.mail.eds.com [199.228.142.91])
3, 25 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAII4bLb009755
3, 25 --     for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:38 -0600
3, 25 -- Received: from plmlir5.mail.eds.com (plmlir5-2.mail.eds.com [199.228.142.135])
3, 25 --     by plmler11.mail.eds.com (8.14.2/8.13.8) with ESMTP id nAII4bmB019828
3, 25 --     for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:37 -0600
3, 25 -- Received: from plmlir5.mail.eds.com (localhost [127.0.0.1])
3, 25 --     by plmlir5.mail.eds.com (8.14.2/8.12.10) with ESMTP id nAII4Rk5024742
3, 25 --     for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:27 -0600
3, 25 -- Received: from USAT0EM0AD01.MAIL.ATI.INT (USAT0EM0AD01.us.ad.ati.int [143.242.253.24])
3, 25 --     by plmlir5.mail.eds.com (8.14.2/8.12.10) with ESMTP id nAII4RPM024729
3, 25 --     for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 12:04:27 -0600
3, 25 -- X-EDSINT-Source-Ip: 143.242.253.24
3, 25 -- To: microscopy-at-microscopy.com
3, 25 -- Subject: [Microscopy] EDS in TEM - SiLi and SDD detectors
3, 25 -- MIME-Version: 1.0
3, 25 -- X-Mailer: Lotus Notes Release 6.5.4 HF1151 March 27, 2007
3, 25 -- Message-ID: {OF57090C14.5689DAA6-ON85257672.0062A63C-85257672.00634898-at-mail.ati.int}
3, 25 -- From: richard.ross-at-allisontransmission.com
3, 25 -- Date: Wed, 18 Nov 2009 13:04:24 -0500
3, 25 -- X-MIMETrack: Serialize by Router on USAT0EM0AD01/ADMINHG/ATISERVER/ATI(Release 8.0.1|February
3, 25 --  07, 2008) at 11/18/2009 01:04:27 PM,
3, 25 --     Serialize complete at 11/18/2009 01:04:27 PM
3, 25 -- Content-Type: text/plain; charset="US-ASCII"
==============================End of - Headers==============================






==============================Original Headers==============================
18, 25 -- From nizets2-at-yahoo.com Thu Nov 19 07:18:35 2009
18, 25 -- Received: from web110811.mail.gq1.yahoo.com (web110811.mail.gq1.yahoo.com [67.195.13.234])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAJDIY5Q030218
18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 07:18:35 -0600
18, 25 -- Received: (qmail 11399 invoked by uid 60001); 19 Nov 2009 13:18:34 -0000
18, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1258636714; bh=XZTaksHg/CeK42HotWfgjQjJan3YFQoPaV/F/WMG/jU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=Wdl/3xz2lJNF78MJVUiwqlpPSUy/uRX1anUP3Hb/aZUAX/L31up/iNRqYg8RhLUPEjKqYUykDbnBL9DmAueuyPNlfdW+c6DhqEPahvbYzTHfl4Q+2To7/TpFAdi96odjEGC5/tr8QlzVDpWoT30mV3SBtx/1WnZJZgU1P9daia0=
18, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
18, 25 -- s=s1024; d=yahoo.com;
18, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
18, 25 -- b=dx/X/80yXoTLRHA48BXqPPVDWg23ojww3VlLBRUfSvhmEOxFVtfG+y1S3U5aZJCIK72KCVdBZmXRxaMTiWbYUkD47e51KUr/PWTDLLnNzmehI4ixWq3pDGWa+EPwQqSPpEbAyE8yLzxPKE+EDGM70TvLbxkHk/zCv1i6Eng2Bz0=;
18, 25 -- Message-ID: {255424.11375.qm-at-web110811.mail.gq1.yahoo.com}
18, 25 -- X-YMail-OSG: CJGM66cVM1lFqWtib7nKTEwvQ8BLl1iKGj2t5hjTmDJms58NuWVMg7wZVY1lUSJYqCo4L07C96OBFeRiF7Mf7mxPr_zyxqthOyZE7BlZ2p_CHWupZc5FiOrn74FjhQszsTex3ZaMhPskBDMYSOEbflmLLb9LtJQpkt.Rh_3.qM7NJzEoGOMiSmZP_Z.MQew.HEYumc0OYPIpt.opLcl2EDO3gFI2N.iyNPA7e1FNsGg1InRpHls8UYGZQfUF_JY2RYaUe6M4e01.5sw1lvRPkkeItcMYi_icaqp8nZfgHpvw4wxpT5y4nyOrNuQXDjlt2NXrVQGXZFHuus84F1MPyGap4asiC6uJ6PjfTvUSLCAks5S9n3ss1E2yEQ--
18, 25 -- Received: from [80.122.101.100] by web110811.mail.gq1.yahoo.com via HTTP; Thu, 19 Nov 2009 05:18:33 PST
18, 25 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.8.100.260964
18, 25 -- References: {200911181808.nAII8aQN016083-at-ns.microscopy.com}
18, 25 -- Date: Thu, 19 Nov 2009 05:18:33 -0800 (PST)
18, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
18, 25 -- Subject: Re: [Microscopy] EDS in TEM - SiLi and SDD detectors
18, 25 -- To: richard.ross-at-allisontransmission.com
18, 25 -- Cc: microscopy-at-microscopy.com
18, 25 -- In-Reply-To: {200911181808.nAII8aQN016083-at-ns.microscopy.com}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain; charset=iso-8859-1
18, 25 -- Content-Transfer-Encoding: 8bit
18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJDIY5Q030218
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 19 Nov 2009 07:28:26 -0600
Subject: [Microscopy] viaWWW: Prevent block face from Concaving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I see no obvious reason for this to happen, at least with Epon (substitutes).
Does this happen systematically in all blocks?
You may have catched an air bubble in the bottom?
Without a precise protocol and no divination power it is very hard to say what could have failed.

regards

Stephane


----- Original Message ----
X-from: "l-reyjr-at-northwestern.edu" {l-reyjr-at-northwestern.edu}
To: nizets2-at-yahoo.com
Sent: Wed, November 18, 2009 10:25:47 PM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both l-reyjr-at-northwestern.edu as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: Northwestern University

Title-Subject: [Filtered] Prevent block face from Concaving

Question: I need your help with this problem. When embedding cell
cultures in LR White or Embed812 and Araldite mixture on coverslips
(glass or thermanox). When I invert beem capsule or gelatin capsule
onto coverslip. Then place block in 60 degree oven. The block surface
becomes concave. Is there anyway to prevent this. When I thin
section, the sectioning begins at the four corners of the block face.

Thanks in Advance
Lennell

  Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Nov 18 15:21:34 2009
7, 11 -- Received: from [129.16.193.210] (91-103-36-171.dynamic.thecloud.net [91.103.36.171])
7, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAILLWwn012317
7, 11 --     for {microscopy-at-microscopy.com} ; Wed, 18 Nov 2009 15:21:33 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240808c72a15c05e97-at-[129.16.193.210]}
7, 11 -- Date: Wed, 18 Nov 2009 22:21:31 +0100
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Prevent block face from Concaving
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
18, 25 -- From nizets2-at-yahoo.com Thu Nov 19 07:28:26 2009
18, 25 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225])
18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAJDSPjs011623
18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 07:28:26 -0600
18, 25 -- Received: (qmail 76802 invoked by uid 60001); 19 Nov 2009 13:28:25 -0000
18, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1258637305; bh=HjdKwwwpi9+JHgj2ZQI78UCv36YL1GQpe7qkzuQK3AM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=cyT8Q4ot0risPwmMTxp1Ctwpv5BHnSyei85uYSYfcWUsDlOoSPPvSaimdrOPCYizhcokDxe4bFqXVJA3ixJFP4jYJXTmT6xbmmlD6xpLDOna3Ygm3oDtCExDC6oKdaywdkUb2lkwm+OxgFidvOcqBtE9LImsLVZrqBN9rXpBaZk=
18, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
18, 25 -- s=s1024; d=yahoo.com;
18, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
18, 25 -- b=ml8nkcnSiYM78vekHMUoVQSYf2GkgU1zG/Y6zmmiKf2VsRbz/S47iQwlCnHUVzivRaHzTLWkALdRefqyaqxOgSpEYfsZT5SfXeqwFczWy0sIT2X3QWS7bWaaHMFXw1JPDSACJYm2U6JdRFmswVrbBNezhiyAF1U/JiuQZ9cjTG0=;
18, 25 -- Message-ID: {451982.76027.qm-at-web110802.mail.gq1.yahoo.com}
18, 25 -- X-YMail-OSG: LgbizCsVM1ndSLjJP90ZRl9miyTeBZyKdK8U3Dj4vWMqpsFG5sM8n1nB.hTMbpQnmuRKP_71VXanENW6BDteZFdL2T.F.bF50AtYSqaCEyuOY59lqfV7ZBDLaLJNWOa6DiHREIkLlgbjqJsFHx4bIa08PC_q_SZg9lfIehdg3KTopolIfvXBkwSl1zBxfrrp2ObVBJvrr30Sx02R1zS.i7QWhUr_aYRKGVWnlG_218GSGB7Lf_Gp8QdVJ9tRgtu63yhAhVWSFoKwCdZH1wCavDEJtU5txPwrK5C8_cKxIX0EwmFxFQf1IwzUbzTh5drxlB0Np7RNF5DYlzzSPzoGdKnE4vyNLGWdLZAuz_JBk7uI
18, 25 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Thu, 19 Nov 2009 05:28:25 PST
18, 25 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.8.100.260964
18, 25 -- References: {200911182125.nAILPl1W018574-at-ns.microscopy.com}
18, 25 -- Date: Thu, 19 Nov 2009 05:28:25 -0800 (PST)
18, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
18, 25 -- Subject: Re: [Microscopy] viaWWW: Prevent block face from Concaving
18, 25 -- To: l-reyjr-at-northwestern.edu
18, 25 -- Cc: microscopy-at-microscopy.com
18, 25 -- In-Reply-To: {200911182125.nAILPl1W018574-at-ns.microscopy.com}
18, 25 -- MIME-Version: 1.0
18, 25 -- Content-Type: text/plain; charset=iso-8859-1
18, 25 -- Content-Transfer-Encoding: 8bit
18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJDSPjs011623
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Thu, 19 Nov 2009 08:02:24 -0600
Subject: [Microscopy] Oxford MDX 1000 X-Ray Fluorescence Spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,
Can anyone tell me anything about this XRF unit? I tried to Google it,
but didn't get many viable hits. My questions are - How old is this
product line? Does the
manufacturer still support it for service and parts? Since it has an
x-ray tube, I assume it has to be certified by the state gov't for
safety. I tried calling Oxford Instruments, they never returned my
call........ Replies off-line are OK.

Gary Easton, Pres.
Scanners Corporation
410-857-7633 x102
--


==============================Original Headers==============================
3, 20 -- From garyeaston-at-scannerscorp.com Thu Nov 19 08:02:23 2009
3, 20 -- Received: from omr8.networksolutionsemail.com (omr8.networksolutionsemail.com [205.178.146.58])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJE2Nu8028723
3, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 08:02:23 -0600
3, 20 -- Received: from cm-omr1 (mail.networksolutionsemail.com [205.178.146.50])
3, 20 -- by omr8.networksolutionsemail.com (8.13.6/8.13.6) with ESMTP id nAJE2NHb026805
3, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 09:02:23 -0500
3, 20 -- Received: from [72.81.190.77] ([72.81.190.77:62077] helo=[192.168.1.3])
3, 20 -- by cm-omr1 (envelope-from {garyeaston-at-scannerscorp.com} )
3, 20 -- (ecelerity 2.2.2.41 r(31179/31189)) with ESMTP
3, 20 -- id 7C/56-24078-EEF450B4; Thu, 19 Nov 2009 09:02:23 -0500
3, 20 -- Message-ID: {4B054FF0.5000508-at-scannerscorp.com}
3, 20 -- Date: Thu, 19 Nov 2009 09:02:24 -0500
3, 20 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 20 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 20 -- MIME-Version: 1.0
3, 20 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 20 -- Subject: Oxford MDX 1000 X-Ray Fluorescence Spectrometer
3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Thu, 19 Nov 2009 08:26:31 -0600
Subject: [Microscopy] Re: viaWWW: Prevent block face from Concaving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Linnell

Frustrating, isn't it. Be assured to know that you're not alone. In
the 25 years since I first cut monolayers I can't remember any time
where this has not happened (how's that for double negatives) -
regardless of plastic, regardless of whether it is a glass or thermonox
substrate. The concave nature is not extreme, never more than
1-2microns, but it seems to be always there. I assume this is due to
contraction of plastic during polymerization, but have no way of proving
that. Perhaps a wiser head can tell us how to prevent this.


Paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
7, 21 -- From paul_hazelton-at-umanitoba.ca Thu Nov 19 08:26:30 2009
7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJEQUKK011886
7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 08:26:30 -0600
7, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
7, 21 -- (authenticated bits=0)
7, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id nAJEQUZc026237;
7, 21 -- Thu, 19 Nov 2009 08:26:30 -0600 (CST)
7, 21 -- Message-ID: {4B055593.1060108-at-umanitoba.ca}
7, 21 -- Date: Thu, 19 Nov 2009 08:26:27 -0600
7, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
7, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
7, 21 -- MIME-Version: 1.0
7, 21 -- To: l-reyjr-at-northwestern.edu,
7, 21 -- Microscopy Listserver {microscopy-at-microscopy.com}
7, 21 -- Subject: Re: [Microscopy] viaWWW: Prevent block face from Concaving
7, 21 -- References: {200911182123.nAILNPT2015028-at-ns.microscopy.com}
7, 21 -- In-Reply-To: {200911182123.nAILNPT2015028-at-ns.microscopy.com}
7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 21 -- Content-Transfer-Encoding: 7bit
7, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 19 Nov 2009 08:35:38 -0600
Subject: [Microscopy] need suggestions for replacing /upgrading the XL30 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fred,
I certainly hope you're at least going to submit a summary of your results.
This is very much the kind of information that should be on the Listserver.
After all, what you're trying to avoid is replacing equipment that still has
decades of life left in it because one inexpensive part was obsolete before
it was installed. I think there might just be one or two others out there
who would like to know what you get for replies.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: fahayes-at-ucdavis.edu [mailto:fahayes-at-ucdavis.edu]
Sent: Wednesday, November 18, 2009 7:49 PM
To: kenconverse-at-qualityimages.biz

Does anyone out there have any suggestions regarding replacing/upgrading
the pc for the XL30 SFEG SEM? Please respond offline

Thank you

--
Fred A. Hayes
Manager, Central Facilities
Department of Chemical Engineering and Material Sciences
3118 Bainer Hall
Bainer Hall Drive
UC Davis
Davis, CA 95616
530-752-0284 office
530-754-6350 fax
707-761-9045 cell
fahayes-at-ucdavis.edu
http://www.matscicf.ucdavis.edu/









==============================Original Headers==============================
11, 19 -- From fahayes-at-ucdavis.edu Wed Nov 18 18:45:55 2009
11, 19 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu
[128.120.32.129])
11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAJ0js4f001915
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Nov 2009 18:45:55
-0600
11, 19 -- Received: from [169.237.230.148] ([169.237.230.148])
11, 19 -- by smtp3.ucdavis.edu (8.13.1/8.13.1/it-cyrus-5.2.0) with
ESMTP id nAJ0jrfB061842
11, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Nov 2009 16:45:53
-0800
11, 19 -- Message-ID: {4B049543.1020208-at-ucdavis.edu}
11, 19 -- Date: Wed, 18 Nov 2009 16:45:55 -0800
11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu}
11, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
11, 19 -- MIME-Version: 1.0
11, 19 -- To: Microscopy-at-Microscopy.Com
11, 19 -- Subject: need suggestions for replacing /upgrading the XL30 SEM pc
11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
11, 19 -- Content-Transfer-Encoding: 7bit
11, 19 -- X-Virus-Scanned: clamav-milter 0.95.1 at av8
11, 19 -- X-Virus-Status: Clean
11, 19 -- X-Scanned-By: MIMEDefang 2.67 on 128.120.32.8
==============================End of - Headers==============================




==============================Original Headers==============================
23, 25 -- From kenconverse-at-qualityimages.biz Thu Nov 19 08:35:38 2009
23, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJEZcQi026613
23, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 08:35:38 -0600
23, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com
23, 25 -- with ESMTP
23, 25 -- id {20091119143536958.ZKLO2218-at-cdptpa-omta01.mail.rr.com} ;
23, 25 -- Thu, 19 Nov 2009 14:35:36 +0000
23, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
23, 25 -- To: {fahayes-at-ucdavis.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
23, 25 -- Subject: RE: [Microscopy] need suggestions for replacing /upgrading the XL30 SEM pc
23, 25 -- Date: Thu, 19 Nov 2009 09:35:28 -0500
23, 25 -- Message-ID: {4604139CAC964940902E9840E663707B-at-Ken}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain;
23, 25 -- charset="us-ascii"
23, 25 -- X-Priority: 3 (Normal)
23, 25 -- X-MSMail-Priority: Normal
23, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
23, 25 -- In-Reply-To: {200911190048.nAJ0mcZZ005717-at-ns.microscopy.com}
23, 25 -- Importance: Normal
23, 25 -- Thread-Index: Acposgm3MVGDPX4RRya9Oh/EmnrAyQAcrgUg
23, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJEZcQi026613
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 19 Nov 2009 08:39:17 -0600
Subject: [Microscopy] viaWWW: Prevent block face from Concaving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Do you use a BEEM capsule filled with unpolymerized epoxy? I think I
have read this method that says to invert a filled BEEM capsule over the
cells on the slide.... Epoxy always shrinks, some formulas more than
others, but always a filled BEEM capsule has a hollow at the top. So if
you used a pre-filled and polymerized beem cap and fill the hollow with
fresh resin and invert the shrinkage should be proportionately less -
proportional to the new volume..

A professor I used to work for had a device that held a polymerized BEEM
stub with a dot of new resin against a semi-thin section on a slide;
after polymerization the slide was popped with LN2 and the the semi-thin
could be resectioned; a similar idea...

Dale

paul_hazelton-at-umanitoba.ca wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Linnell
}
} Frustrating, isn't it. Be assured to know that you're not alone. In
} the 25 years since I first cut monolayers I can't remember any time
} where this has not happened (how's that for double negatives) -
} regardless of plastic, regardless of whether it is a glass or thermonox
} substrate. The concave nature is not extreme, never more than
} 1-2microns, but it seems to be always there. I assume this is due to
} contraction of plastic during polymerization, but have no way of proving
} that. Perhaps a wiser head can tell us how to prevent this.
}
}
} Paul
}

==============================Original Headers==============================
4, 23 -- From dac-at-research.umass.edu Thu Nov 19 08:39:16 2009
4, 23 -- Received: from race-5.oit.umass.edu (race-5.oit.umass.edu [128.119.2.116])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJEdGGB002762
4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 08:39:16 -0600
4, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30])
4, 23 -- (authenticated bits=0)
4, 23 -- by race-5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id nAJEdFqd020692
4, 23 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT);
4, 23 -- Thu, 19 Nov 2009 09:39:16 -0500
4, 23 -- Message-ID: {4B055898.4070201-at-research.umass.edu}
4, 23 -- Date: Thu, 19 Nov 2009 09:39:20 -0500
4, 23 -- From: Dale Callaham {dac-at-research.umass.edu}
4, 23 -- Reply-To: dac-at-research.umass.edu
4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.1.4) Gecko/20091017 SeaMonkey/2.0
4, 23 -- MIME-Version: 1.0
4, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
4, 23 -- l-reyjr-at-northwestern.edu
4, 23 -- Subject: Re: [Microscopy] Re: viaWWW: Prevent block face from Concaving
4, 23 -- References: {200911191430.nAJEUwfH019681-at-ns.microscopy.com}
4, 23 -- In-Reply-To: {200911191430.nAJEUwfH019681-at-ns.microscopy.com}
4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- X-Whitelist: TRUE
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Thu, 19 Nov 2009 09:03:35 -0600
Subject: [Microscopy] SEM service providers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are looking for contact information on companies or individuals that
service SEMs, including FESEMs in the New England (USA) area. Any
information will be gratefully received.

Cheers

Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5453
fax: 860-486-4745




==============================Original Headers==============================
5, 22 -- From raristau-at-ims.uconn.edu Thu Nov 19 09:03:35 2009
5, 22 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJF3ZM8024126
5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Nov 2009 09:03:35 -0600
5, 22 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu [137.99.20.157])
5, 22 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id nAJF3VDi004221
5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Nov 2009 10:03:31 -0500
5, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122
5, 22 -- Date: Thu, 19 Nov 2009 10:03:31 -0500
5, 22 -- Subject: SEM service providers
5, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
5, 22 -- To: {Microscopy-at-Microscopy.Com}
5, 22 -- Message-ID: {C72AC873.98C%raristau-at-ims.uconn.edu}
5, 22 -- Thread-Topic: SEM service providers
5, 22 -- Thread-Index: AcppKXTwszppSdUcEd6f6wAbY55CVA==
5, 22 -- Mime-version: 1.0
5, 22 -- Content-type: text/plain;
5, 22 -- charset="US-ASCII"
5, 22 -- Content-transfer-encoding: 7bit
5, 22 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
5, 22 -- X-UConn-MailScanner: Found to be clean
5, 22 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: jehrman-at-mta.ca
Date: Thu, 19 Nov 2009 10:14:11 -0600
Subject: [Microscopy] air table gas consumption

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good day listers,

We've just had a Kinetic air table delivered for our new Zeiss Apotome
scope and I'm casting about for a source of air for the table. Anybody
out there have an idea how much gas is consumed by such a table. How
often (roughly) would we need to change a standard nitrogen cylinder?
Alternatively, if we provided air with a compressor, how often could we
expect the compressor to cycle in the course of a day? We're mainly
concerned with noise, and the compressor provided by the vendor seems a
little expensive. Can we get away with a less expensive solution?

As usual, thanks in advance,


Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Svent-Gyorgyi's Axiom:
Discovery consists of seeing what everybody
has seen and thinking what nobody thought.


==============================Original Headers==============================
10, 18 -- From jehrman-at-mta.ca Thu Nov 19 10:14:11 2009
10, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.208])
10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJGEBdM008974
10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:14:11 -0600
10, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:63368)
10, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
10, 18 -- with esmtp id 1NB9e6-00030J-CB (Exim 4.69) for Microscopy-at-microscopy.com
10, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 19 Nov 2009 12:14:10 -0400
10, 18 -- Message-ID: {4B056E97.7040309-at-mta.ca}
10, 18 -- Date: Thu, 19 Nov 2009 12:13:11 -0400
10, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
10, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 18 -- MIME-Version: 1.0
10, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
10, 18 -- Subject: air table gas consumption
10, 18 -- X-Enigmail-Version: 0.96.0
10, 18 -- Content-Type: text/plain; charset=ISO-8859-1
10, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Thu, 19 Nov 2009 10:26:36 -0600
Subject: [Microscopy] air table gas consumption

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are fortunate to have gas/vac/air available in all of our hoods so
we just tapped into the line for air and added a pressure regulator on
that line for the anti-vibration table. Any chance of doing that in
your building?

best,
Beth

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Good day listers,
}
} We've just had a Kinetic air table delivered for our new Zeiss Apotome
} scope and I'm casting about for a source of air for the table. Anybody
} out there have an idea how much gas is consumed by such a table. How
} often (roughly) would we need to change a standard nitrogen cylinder?
} Alternatively, if we provided air with a compressor, how often could
} we
} expect the compressor to cycle in the course of a day? We're mainly
} concerned with noise, and the compressor provided by the vendor
} seems a
} little expensive. Can we get away with a less expensive solution?
}
} As usual, thanks in advance,
} Jim
}
} --
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/dmf
}
} Svent-Gyorgyi's Axiom:
} Discovery consists of seeing what everybody
} has seen and thinking what nobody thought.



**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
Miller Plant Sciences Bldg
120 Carlton Street
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com






==============================Original Headers==============================
16, 20 -- From beth-at-plantbio.uga.edu Thu Nov 19 10:26:35 2009
16, 20 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJGQZMv023915
16, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:26:35 -0600
16, 20 -- Received: from [128.192.26.46] ([128.192.26.46])
16, 20 -- (authenticated user beth-at-plantbio.uga.edu)
16, 20 -- by dogwood.plantbio.uga.edu
16, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
16, 20 -- for microscopy-at-microscopy.com;
16, 20 -- Thu, 19 Nov 2009 11:26:30 -0500
16, 20 -- Message-Id: {D629054C-14C3-4B88-9F17-51E9E49BAA21-at-plantbio.uga.edu}
16, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
16, 20 -- To: microscopy microscopy {microscopy-at-microscopy.com}
16, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
16, 20 -- Content-Transfer-Encoding: 7bit
16, 20 -- Mime-Version: 1.0 (Apple Message framework v936)
16, 20 -- Subject: [Microscopy] air table gas consumption
16, 20 -- Date: Thu, 19 Nov 2009 11:26:29 -0500
16, 20 -- References: {200911191615.nAJGF0PP009842-at-ns.microscopy.com}
16, 20 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: Michal.Jarnik-at-fccc.edu
Date: Thu, 19 Nov 2009 10:34:38 -0600
Subject: [Microscopy] Reynolds Lead Citrate storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I know this was discussed repeatedly, but anyway. I am currently using 5
or 10 ml syringes, 4°C, for storing lead citrate for post-staining of
thin sections, but not really sure that is the best. Any smart
suggestions? I remember some people store it at RT under mineral oil or
maybe there are other options?

Thanks, Michael


==============================Original Headers==============================
3, 21 -- From Michal.Jarnik-at-fccc.edu Thu Nov 19 10:34:38 2009
3, 21 -- Received: from eclipse.fccc.edu (mx.fccc.edu [131.249.80.52])
3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJGYcRh006201
3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:34:38 -0600
3, 21 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237])
3, 21 -- by eclipse.fccc.edu (8.13.6/8.13.6) with ESMTP id nAJGYZDQ010786
3, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 11:34:35 -0500 (EST)
3, 21 -- Received: from emf1.dyn.fccc.edu (emf1.dyn.fccc.edu [10.40.13.206])
3, 21 -- (authenticated bits=0)
3, 21 -- by azah.fccc.edu (8.13.6/8.13.6) with ESMTP id nAJGYZt0021176
3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 11:34:35 -0500 (EST)
3, 21 -- Message-ID: {4B05739B.4000108-at-fccc.edu}
3, 21 -- Date: Thu, 19 Nov 2009 11:34:35 -0500
3, 21 -- From: Michal Jarnik {Michal.Jarnik-at-fccc.edu}
3, 21 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
3, 21 -- MIME-Version: 1.0
3, 21 -- To: microscopy-at-microscopy.com
3, 21 -- Subject: Reynolds Lead Citrate storage
3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 21 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Thu, 19 Nov 2009 10:58:45 -0600
Subject: [Microscopy] Re: Reynolds Lead Citrate storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI Michael-
we use the Vennable & Coggeshal formulation for
lead citrate. We store it in a foil-wrapped
syringe at room temperature and use a 0.2micron
filter on the syringe when dispensing.
Lee


} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org


==============================Original Headers==============================
6, 35 -- From lcgould-at-med.cornell.edu Thu Nov 19 10:58:45 2009
6, 35 -- Received: from mail-gw1.med.cornell.edu (mail-gw1.med.cornell.edu [140.251.3.44])
6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJGwjQY021917
6, 35 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:58:45 -0600
6, 35 -- MIME-version: 1.0
6, 35 -- Content-type: text/plain; charset=iso-8859-1; format=flowed
6, 35 -- Received: from mpx5.med.cornell.edu ([140.251.11.120])
6, 35 -- by mail-gw1.med.cornell.edu
6, 35 -- (Sun Java(tm) System Messaging Server 6.3-8.03 (built Apr 24 2009; 32bit))
6, 35 -- with ESMTP id {0KTD0036P8HWHO10-at-mail-gw1.med.cornell.edu} for
6, 35 -- microscopy-at-microscopy.com; Thu, 19 Nov 2009 11:58:45 -0500 (EST)
6, 35 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
6, 35 -- by mpx5.med.cornell.edu
6, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.04 64bit (built Jul 2 2009))
6, 35 -- with ESMTPA id {0KTD00FAN8HU6R80-at-mpx5.med.cornell.edu} ; Thu,
6, 35 -- 19 Nov 2009 11:58:44 -0500 (EST)
6, 35 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379,
6, 35 -- Antispam-Data: 2009.11.19.164533
6, 35 -- X-Perlmx-Spam: Gauge=X, , Probability=10%, Report=' TO_IN_SUBJECT 0.5,
6, 35 -- BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0,
6, 35 -- BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0,
6, 35 -- __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0,
6, 35 -- __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MEDS_PLAIN 0,
6, 35 -- __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0,
6, 35 -- __SANE_MSGID 0, __TO_MALFORMED_2 0, __URI_NS '
6, 35 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
6, 35 -- Message-id: {p06230909c72b294b12f4-at-[140.251.48.23]}
6, 35 -- In-reply-to: {200911191641.nAJGfV4j020296-at-ns.microscopy.com}
6, 35 -- References: {200911191641.nAJGfV4j020296-at-ns.microscopy.com}
6, 35 -- Date: Thu, 19 Nov 2009 11:58:40 -0500
6, 35 -- To: Michal.Jarnik-at-fccc.edu, Microscopy Listserver {microscopy-at-microscopy.com}
6, 35 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
6, 35 -- Subject: Re: [Microscopy] Reynolds Lead Citrate storage
6, 35 -- Content-Transfer-Encoding: 8bit
6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJGwjQY021917
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Thu, 19 Nov 2009 11:55:25 -0600
Subject: [Microscopy] air table gas consumption

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jim,
Air consumption is going to be rather variable depending upon whether you
have a large floor mounted table or a benchtop table, how much you actually
touch the scope while using, and how much of the time it is being used.
I've seen SEMs on large floor mounted Micro-G tables that would go for many
months, sometimes years, on a single 220 cu. Ft. nitrogen tank. Other
times, they would only last a couple of months.

If they are trying to sell you a Jun-Aire compressor, I can tell you that
they make all the racket of a modern refrigerator. If noise is an issue, it
is a good solution. The only noise you will generally hear is the unloader
valve at the end of a pump cycle. These compressors use the same basic
compressor that a refrigeration unit uses, rather than a piston or diaphragm
pump. They must be heard to be believed.

No interest other than I like customer sites that have them. No noise, no
high pressure tanks.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca]
Sent: Thursday, November 19, 2009 11:17 AM
To: kenconverse-at-qualityimages.biz

Good day listers,

We've just had a Kinetic air table delivered for our new Zeiss Apotome
scope and I'm casting about for a source of air for the table. Anybody
out there have an idea how much gas is consumed by such a table. How
often (roughly) would we need to change a standard nitrogen cylinder?
Alternatively, if we provided air with a compressor, how often could we
expect the compressor to cycle in the course of a day? We're mainly
concerned with noise, and the compressor provided by the vendor seems a
little expensive. Can we get away with a less expensive solution?

As usual, thanks in advance,


Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Svent-Gyorgyi's Axiom:
Discovery consists of seeing what everybody
has seen and thinking what nobody thought.


==============================Original Headers==============================
10, 18 -- From jehrman-at-mta.ca Thu Nov 19 10:14:11 2009
10, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.208])
10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAJGEBdM008974
10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:14:11
-0600
10, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:63368)
10, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
10, 18 -- with esmtp id 1NB9e6-00030J-CB (Exim 4.69) for
Microscopy-at-microscopy.com
10, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 19 Nov 2009 12:14:10
-0400
10, 18 -- Message-ID: {4B056E97.7040309-at-mta.ca}
10, 18 -- Date: Thu, 19 Nov 2009 12:13:11 -0400
10, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
10, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 18 -- MIME-Version: 1.0
10, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
10, 18 -- Subject: air table gas consumption
10, 18 -- X-Enigmail-Version: 0.96.0
10, 18 -- Content-Type: text/plain; charset=ISO-8859-1
10, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




==============================Original Headers==============================
24, 25 -- From kenconverse-at-qualityimages.biz Thu Nov 19 11:55:25 2009
24, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJHtP1M005996
24, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 11:55:25 -0600
24, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
24, 25 -- with ESMTP
24, 25 -- id {20091119175522368.IEPQ13252-at-cdptpa-omta04.mail.rr.com} ;
24, 25 -- Thu, 19 Nov 2009 17:55:22 +0000
24, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
24, 25 -- To: {jehrman-at-mta.ca} , "MSA Listserver" {microscopy-at-microscopy.com}
24, 25 -- Subject: RE: [Microscopy] air table gas consumption
24, 25 -- Date: Thu, 19 Nov 2009 12:55:13 -0500
24, 25 -- Message-ID: {562342E1F29547639D62898233606972-at-Ken}
24, 25 -- MIME-Version: 1.0
24, 25 -- Content-Type: text/plain;
24, 25 -- charset="us-ascii"
24, 25 -- X-Priority: 3 (Normal)
24, 25 -- X-MSMail-Priority: Normal
24, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
24, 25 -- In-Reply-To: {200911191616.nAJGGhib012843-at-ns.microscopy.com}
24, 25 -- Importance: Normal
24, 25 -- Thread-Index: AcppM7EpyXwande9S7+jcKJkdhnZ2AAC+pIA
24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 25 -- Content-Transfer-Encoding: 8bit
24, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJHtP1M005996
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Thu, 19 Nov 2009 15:08:29 -0600
Subject: [Microscopy] air table gas consumption

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jun-Aire's are definitely great, just very expensive. We used a $290
compressor from Home Depot for quite some time on our 2010F when I ws at
Intel and located it in an equipment chase. The only caveat with the cheap
compressor is that if you are actually consuming air through leaks or air
table actuation you must be diligent about draining the water from the
reservoir on a regular basis, or it will fill up and pump water into the air
table pistons, which really creates a mess. Also persistent water retention
in the reservoir can lead to corrosion of the reservoir which can lead to
the reservoir bursting.

John Mardinly
Western Digital

-----Original Message-----
X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz]
Sent: Thursday, November 19, 2009 10:04 AM
To: John Mardinly

Jim,
Air consumption is going to be rather variable depending upon whether you
have a large floor mounted table or a benchtop table, how much you actually
touch the scope while using, and how much of the time it is being used.
I've seen SEMs on large floor mounted Micro-G tables that would go for many
months, sometimes years, on a single 220 cu. Ft. nitrogen tank. Other
times, they would only last a couple of months.

If they are trying to sell you a Jun-Aire compressor, I can tell you that
they make all the racket of a modern refrigerator. If noise is an issue, it
is a good solution. The only noise you will generally hear is the unloader
valve at the end of a pump cycle. These compressors use the same basic
compressor that a refrigeration unit uses, rather than a piston or diaphragm
pump. They must be heard to be believed.

No interest other than I like customer sites that have them. No noise, no
high pressure tanks.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca]
Sent: Thursday, November 19, 2009 11:17 AM
To: kenconverse-at-qualityimages.biz

Good day listers,

We've just had a Kinetic air table delivered for our new Zeiss Apotome
scope and I'm casting about for a source of air for the table. Anybody
out there have an idea how much gas is consumed by such a table. How
often (roughly) would we need to change a standard nitrogen cylinder?
Alternatively, if we provided air with a compressor, how often could we
expect the compressor to cycle in the course of a day? We're mainly
concerned with noise, and the compressor provided by the vendor seems a
little expensive. Can we get away with a less expensive solution?

As usual, thanks in advance,


Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Svent-Gyorgyi's Axiom:
Discovery consists of seeing what everybody
has seen and thinking what nobody thought.


==============================Original Headers==============================
10, 18 -- From jehrman-at-mta.ca Thu Nov 19 10:14:11 2009
10, 18 -- Received: from mailgate1.mta.ca (mailgate1.mta.ca [138.73.1.208])
10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAJGEBdM008974
10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:14:11
-0600
10, 18 -- Received: from host-22-194.mta.ca ([138.73.22.194]:63368)
10, 18 -- by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25)
10, 18 -- with esmtp id 1NB9e6-00030J-CB (Exim 4.69) for
Microscopy-at-microscopy.com
10, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 19 Nov 2009 12:14:10
-0400
10, 18 -- Message-ID: {4B056E97.7040309-at-mta.ca}
10, 18 -- Date: Thu, 19 Nov 2009 12:13:11 -0400
10, 18 -- From: "James M. Ehrman" {jehrman-at-mta.ca}
10, 18 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 18 -- MIME-Version: 1.0
10, 18 -- To: Microscopy Listserv {Microscopy-at-microscopy.com}
10, 18 -- Subject: air table gas consumption
10, 18 -- X-Enigmail-Version: 0.96.0
10, 18 -- Content-Type: text/plain; charset=ISO-8859-1
10, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




==============================Original Headers==============================
24, 25 -- From kenconverse-at-qualityimages.biz Thu Nov 19 11:55:25 2009
24, 25 -- Received: from cdptpa-omtalb.mail.rr.com
(cdptpa-omtalb.mail.rr.com [75.180.132.123])
24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAJHtP1M005996
24, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 11:55:25
-0600
24, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
24, 25 -- with ESMTP
24, 25 -- id
{20091119175522368.IEPQ13252-at-cdptpa-omta04.mail.rr.com} ;
24, 25 -- Thu, 19 Nov 2009 17:55:22 +0000
24, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
24, 25 -- To: {jehrman-at-mta.ca} , "MSA Listserver" {microscopy-at-microscopy.com}
24, 25 -- Subject: RE: [Microscopy] air table gas consumption
24, 25 -- Date: Thu, 19 Nov 2009 12:55:13 -0500
24, 25 -- Message-ID: {562342E1F29547639D62898233606972-at-Ken}
24, 25 -- MIME-Version: 1.0
24, 25 -- Content-Type: text/plain;
24, 25 -- charset="us-ascii"
24, 25 -- X-Priority: 3 (Normal)
24, 25 -- X-MSMail-Priority: Normal
24, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
24, 25 -- In-Reply-To: {200911191616.nAJGGhib012843-at-ns.microscopy.com}
24, 25 -- Importance: Normal
24, 25 -- Thread-Index: AcppM7EpyXwande9S7+jcKJkdhnZ2AAC+pIA
24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
24, 25 -- Content-Transfer-Encoding: 8bit
24, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nAJHtP1M005996
==============================End of - Headers==============================


==============================Original Headers==============================
31, 26 -- From John.Mardinly-at-wdc.com Thu Nov 19 15:08:29 2009
31, 26 -- Received: from wdscexfe01.sc.wdc.com (wdscexfe01.sc.wdc.com [129.253.170.53])
31, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJL8Slr028121
31, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 15:08:29 -0600
31, 26 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
31, 26 -- Thu, 19 Nov 2009 13:08:28 -0800
31, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
31, 26 -- Content-class: urn:content-classes:message
31, 26 -- MIME-Version: 1.0
31, 26 -- Content-Type: text/plain;
31, 26 -- charset="us-ascii"
31, 26 -- Subject: RE: [Microscopy] RE: air table gas consumption
31, 26 -- Date: Thu, 19 Nov 2009 13:08:27 -0800
31, 26 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E118EC-at-wdksjexbe01.msj.wdc.com}
31, 26 -- In-Reply-To: {200911191803.nAJI3Vpx020067-at-ns.microscopy.com}
31, 26 -- X-MS-Has-Attach:
31, 26 -- X-MS-TNEF-Correlator:
31, 26 -- Thread-Topic: [Microscopy] RE: air table gas consumption
31, 26 -- Thread-Index: AcppQpwsN7Lwjt8HQ0eHvijsNzmCWAAGPaJQ
31, 26 -- References: {200911191803.nAJI3Vpx020067-at-ns.microscopy.com}
31, 26 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
31, 26 -- To: {kenconverse-at-qualityimages.biz}
31, 26 -- Cc: {Microscopy-at-microscopy.com}
31, 26 -- X-OriginalArrivalTime: 19 Nov 2009 21:08:28.0191 (UTC) FILETIME=[70AF12F0:01CA695C]
31, 26 -- Content-Transfer-Encoding: 8bit
31, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJL8Slr028121
==============================End of - Headers==============================




From: wadowska-at-upei.ca
Date: Thu, 19 Nov 2009 15:16:15 -0600
Subject: [Microscopy] viaWWW: TEM cleaning column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: University od PEI

Title-Subject: [Filtered] TEM cleaning column

Question: Hello,
I would like to know if it is necessary to clean a column in TEM
after collapse of a turbo molecular pump? We have to change
flourescent screen because of a debris fall out so I am wondering if
entire column might be contaminated.
Thank you
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Nov 19 15:16:14 2009
6, 11 -- Received: from [10.158.144.171] (91-103-36-171.dynamic.thecloud.net [91.103.36.171])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJLGC1O004398
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 15:16:14 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c72b660b221c-at-[129.16.193.210]}
6, 11 -- Date: Thu, 19 Nov 2009 22:16:10 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM cleaning column
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 19 Nov 2009 15:21:00 -0600
Subject: [Microscopy] viaWWW: TEM cleaning column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Short answer--yes, based on my experience. The coating from the screen probably is scattered up and down the whole column.

Good luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com



-----Original Message-----
X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
Sent: Thursday, November 19, 2009 3:17 PM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: University od PEI

Title-Subject: [Filtered] TEM cleaning column

Question: Hello,
I would like to know if it is necessary to clean a column in TEM
after collapse of a turbo molecular pump? We have to change
flourescent screen because of a debris fall out so I am wondering if
entire column might be contaminated.
Thank you
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Nov 19 15:16:14 2009
6, 11 -- Received: from [10.158.144.171] (91-103-36-171.dynamic.thecloud.net [91.103.36.171])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJLGC1O004398
6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 15:16:14 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c72b660b221c-at-[129.16.193.210]}
6, 11 -- Date: Thu, 19 Nov 2009 22:16:10 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM cleaning column
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
17, 33 -- From TindallR-at-missouri.edu Thu Nov 19 15:20:59 2009
17, 33 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJLKxRl018612
17, 33 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 15:20:59 -0600
17, 33 -- X-IronPort-Anti-Spam-Filtered: true
17, 33 -- X-IronPort-Anti-Spam-Result: AnsFAM1FBUvRauUo/2dsb2JhbACVDIdsqiwBDAEBAQeFeohPglEBAYFoBA
17, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
17, 33 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 19 Nov 2009 15:20:58 -0600
17, 33 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
17, 33 -- Thu, 19 Nov 2009 15:20:57 -0600
17, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
17, 33 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Thu, 19 Nov 2009
17, 33 -- 15:20:57 -0600
17, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
17, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
17, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
17, 33 -- Date: Thu, 19 Nov 2009 15:20:55 -0600
17, 33 -- Subject: RE: [Microscopy] viaWWW: TEM cleaning column
17, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM cleaning column
17, 33 -- Thread-Index: AcppXaQbRdei6lHcRain00mqcKvB9gAAEICQ
17, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129B19C9EC-at-UM-EMAIL06.um.umsystem.edu}
17, 33 -- References: {200911192117.nAJLH2eC006810-at-ns.microscopy.com}
17, 33 -- In-Reply-To: {200911192117.nAJLH2eC006810-at-ns.microscopy.com}
17, 33 -- Accept-Language: en-US
17, 33 -- Content-Language: en-US
17, 33 -- X-MS-Has-Attach:
17, 33 -- X-MS-TNEF-Correlator:
17, 33 -- acceptlanguage: en-US
17, 33 -- Content-Type: text/plain; charset="us-ascii"
17, 33 -- MIME-Version: 1.0
17, 33 -- X-OriginalArrivalTime: 19 Nov 2009 21:20:57.0964 (UTC) FILETIME=[2F955AC0:01CA695E]
17, 33 -- Content-Transfer-Encoding: 8bit
17, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAJLKxRl018612
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Thu, 19 Nov 2009 16:33:03 -0600
Subject: [Microscopy] viaWWW: TEM cleaning column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, I have seen this as well. And be careful as you clean - I've also
seen fluorescent hand- and footprints all over a lab from this exercise!
The stuff gets everywhere. And if you have any other debris in the column,
you really must get this out as well, especially if magnetic.

Aloha,
Tina

} Short answer--yes, based on my experience. The coating from the
} screen probably is scattered up and down the whole column.
}
} Good luck,
} Randy

} Question: Hello,
} I would like to know if it is necessary to clean a column in TEM
} after collapse of a turbo molecular pump? We have to change
} flourescent screen because of a debris fall out so I am wondering if
} entire column might be contaminated.
} Thank you
} Dorota

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
7, 21 -- From tina-at-pbrc.hawaii.edu Thu Nov 19 16:33:03 2009
7, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAJMX2T4008943
7, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 16:33:03 -0600
7, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
7, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id nAJMWw9J003426
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 21 -- Thu, 19 Nov 2009 12:32:59 -1000 (HST)
7, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id nAJMWtKk003423;
7, 21 -- Thu, 19 Nov 2009 12:32:55 -1000 (HST)
7, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
7, 21 -- Date: Thu, 19 Nov 2009 12:32:54 -1000 (HST)
7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
7, 21 -- X-Sender: tina-at-halia
7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
7, 21 -- cc: wadowska-at-upei.ca
7, 21 -- Subject: Re: [Microscopy] RE: viaWWW: TEM cleaning column
7, 21 -- In-Reply-To: {200911192121.nAJLLuBb021854-at-ns.microscopy.com}
7, 21 -- Message-ID: {Pine.GSO.4.21.0911191228170.3400-100000-at-halia}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: analytic-at-rawbw.com
Date: Fri, 20 Nov 2009 08:45:44 -0600
Subject: [Microscopy] viaWWW: Photos of High School students with an SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow microscopists,

A colleague and I are discussing the possibility of conducting
magnetic measurements on rock samples that have been analyzed in our
electron microprobe. One of the questions that emerged from our
discussion concerns the strength of the magnetic field experienced by
the samples in a microprobe, which, if strong enough, could have reset
certain magnetic parameters within the rock samples. It seems to me
that there should be two components to this. First, there are the
magnetic fields produced by instrument itself. Second, there are the
magnetic fields that are produced by the electron beam -- moving
electrons cause magnetic fields, so they must even produce a magnetic
field in the sample itself. A cursory look through the literature
didn't produce any numbers or equations to estimate this magnetic
field exposure. Does anyone know of estimates (theoretical or
experimental) or equations (presumably beam current and voltage play a
role) for the magnetic field that a sample would experience?

Thanks,
Ellery

-----------------
Ellery Frahm
Senior Research Fellow, Department of Geology & Geophysics
Manager & Principal Analyst, Electron Microprobe Lab
University of Minnesota - Twin Cities campus
Lab website: http://probelab.geo.umn.edu/
Personal: http://web.mac.com/elleryfrahm/







==============================Original Headers==============================
10, 17 -- From frah0010-at-umn.edu Thu Nov 19 18:13:57 2009
10, 17 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232])
10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAK0DvH6026738
10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 18:13:57 -0600
10, 17 -- Received: from [10.0.1.199] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206])
10, 17 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP
10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 18:13:57 -0600 (CST)
10, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN
10, 17 -- Message-Id: {569D0464-7C8A-4FEF-A6E4-46F451D9DC66-at-umn.edu}
10, 17 -- From: Ellery Frahm {frah0010-at-umn.edu}
10, 17 -- To: microscopy-at-microscopy.com
10, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
10, 17 -- Content-Transfer-Encoding: 7bit
10, 17 -- Mime-Version: 1.0 (Apple Message framework v936)
10, 17 -- Subject: magnetic field experienced by samples
10, 17 -- Date: Thu, 19 Nov 2009 18:13:55 -0600
10, 17 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================

From quoteme-at-familyinsuranceservices.com Thu Nov 19 20:02:05 2009
Return-Path: {quoteme-at-familyinsuranceservices.com}
Received: from 84-74-14-75.dclient.hispeed.ch (84-74-14-75.dclient.hispeed.ch [84.74.14.75])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAK2223g014490;
Thu, 19 Nov 2009 20:02:03 -0600
Received: from [84.74.14.75] by exchange.familyinsuranceservices.com; Fri, 20 Nov 2009 02:59:39 +0100

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both analytic-at-rawbw.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: analytic-at-rawbw.com
Name: Margo Gill-Linscott

Organization: Analyticus

Title-Subject: [Filtered] Photos of High School students with an SEM

Question: I would like to include a picture of high school students
with an SEM in a paper. Does anyone have a photo they could send? I
would greatly appreciate any contributions. Thank you. Margo
Linscott

Login Host: 198.144.209.67
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:45:44 2009
6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se [129.16.200.133])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKEjfYw017966
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:45:43 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c72c5c025ce9-at-[10.158.144.171]}
6, 11 -- Date: Fri, 20 Nov 2009 15:45:38 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Photos of High School students with an SEM
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: wadowska-at-upei.ca
Date: Fri, 20 Nov 2009 08:46:09 -0600
Subject: [Microscopy] viaWWW: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: University of PEI

Title-Subject: [Filtered] TEM how to clean column

Question: Hi guys,
Thanks for your input about column and TMP. My next question is: how
to clean a column? Would you do it yourself or leave it to a service
person?
Thank you
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se [129.16.200.133])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKEk79r018312
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM how to clean column
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kaszas.1-at-osu.edu
Date: Fri, 20 Nov 2009 08:46:37 -0600
Subject: [Microscopy] viaWWW: Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kaszas.1-at-osu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kaszas.1-at-osu.edu
Name: Andrea Kaszas

Organization: Ohio State University/OARDC

Title-Subject: [Filtered] Spurr resin

Question: Dear list members,


Does anybody among you use Spurr resin for studying plant materials
by transmisson electron microscopy?
If so, what protocol do you use with the new formulation of ERL 4221.
Has anyone found another resin for plant tissue for studying cell
structure with transmission electron microscopy?
Any suggestions are greatly appreciated.

Andrea Kaszas
Research assistant



Login Host: 164.107.85.175
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:37 2009
11, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se [129.16.200.133])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKEkXhC019360
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:35 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240802c72c5c3969eb-at-[129.16.200.133]}
11, 11 -- Date: Fri, 20 Nov 2009 15:46:31 +0100
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: kaszas.1-at-osu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Spurr resin
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 20 Nov 2009 09:14:33 -0600
Subject: [Microscopy] viaWWW: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't know about others, but there is absolutely no way I would tackle cleaning a TEM column myself. I have done SEM columns in the past, but that's a different critter altogether, seems to me.

Are there labs out there that do this in-house without trained service engineers? I'm curious.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




-----Original Message-----
X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
Sent: Friday, November 20, 2009 8:47 AM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: University of PEI

Title-Subject: [Filtered] TEM how to clean column

Question: Hi guys,
Thanks for your input about column and TMP. My next question is: how
to clean a column? Would you do it yourself or leave it to a service
person?
Thank you
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se [129.16.200.133])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKEk79r018312
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TEM how to clean column
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFEWYj029978
19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32 -0600
19, 33 -- X-IronPort-Anti-Spam-Filtered: true
19, 33 -- X-IronPort-Anti-Spam-Result: AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009 09:14:31 -0600
19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20 Nov 2009
19, 33 -- 09:14:31 -0600
19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
19, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
19, 33 -- Accept-Language: en-US
19, 33 -- Content-Language: en-US
19, 33 -- X-MS-Has-Attach:
19, 33 -- X-MS-TNEF-Correlator:
19, 33 -- acceptlanguage: en-US
19, 33 -- Content-Type: text/plain; charset="us-ascii"
19, 33 -- MIME-Version: 1.0
19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC) FILETIME=[28FBFCA0:01CA69F4]
19, 33 -- Content-Transfer-Encoding: 8bit
19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAKFEWYj029978
==============================End of - Headers==============================




From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 20 Nov 2009 09:29:32 -0600
Subject: [Microscopy] TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} } }
} I don't know about others, but there is absolutely no way I would tackle
} cleaning a TEM column myself. I have done SEM columns in the past, but
} that's a different critter altogether, seems to me.
}
} Are there labs out there that do this in-house without trained service
} engineers? I'm curious.

We do not clean the TEM column ourselves. No way. Far too complicated, IMO, for our group / our department / faculty.
Done by Service people from the manufacturer.
best regards,
Reinhard Rachel


--

PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
-at-Institute for Anatomy
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29



==============================Original Headers==============================
7, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Nov 20 09:29:30 2009
7, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51])
7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFTToN012813
7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:29:30 -0600
7, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1])
7, 25 -- by localhost (Postfix) with SMTP id 21B19B51
7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 16:28:03 +0100 (CET)
7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51])
7, 25 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id D6719B33
7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 16:28:02 +0100 (CET)
7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
7, 25 -- with Novell_GroupWise; Fri, 20 Nov 2009 16:29:29 +0100
7, 25 -- Message-Id: {4B06C3E4020000540000BEA5-at-gwsmtp1.uni-regensburg.de}
7, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.1
7, 25 -- Date: Fri, 20 Nov 2009 16:29:24 +0100
7, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de}
7, 25 -- To: {microscopy-at-microscopy.com}
7, 25 -- Subject: TEM how to clean column
7, 25 -- References: {200911201514.nAKFEi7S030334-at-ns.microscopy.com}
7, 25 -- In-Reply-To: {200911201514.nAKFEi7S030334-at-ns.microscopy.com}
7, 25 -- Mime-Version: 1.0
7, 25 -- Content-Type: text/plain; charset=US-ASCII
7, 25 -- Content-Disposition: inline
7, 25 -- Content-Transfer-Encoding: 8bit
7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAKFTToN012813
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 20 Nov 2009 09:43:45 -0600
Subject: [Microscopy] Re: viaWWW: Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Andrea,

We use the recipe by Ann Ellis, in the
"Solutions to the Problem of Substitution of ERL 4221 for Vinyl
Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
July 2006, volume 14, No.4, pg.32ff
If you don't get MT, this issue can be downloaded from the MT archives at:
http://www.microscopy-today.com/index.faces

Phil

} Email: kaszas.1-at-osu.edu
} Name: Andrea Kaszas
}
} Organization: Ohio State University/OARDC
}
} Title-Subject: [Filtered] Spurr resin
}
} Question: Dear list members,
}
}
} Does anybody among you use Spurr resin for studying plant materials
} by transmisson electron microscopy?
} If so, what protocol do you use with the new formulation of ERL 4221.
} Has anyone found another resin for plant tissue for studying cell
} structure with transmission electron microscopy?
} Any suggestions are greatly appreciated.
}
} Andrea Kaszas
} Research assistant
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 26 -- From oshel1pe-at-cmich.edu Fri Nov 20 09:43:45 2009
4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFhjQS027891
4, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:43:45 -0600
4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nAKFhcDD021006;
4, 26 -- Fri, 20 Nov 2009 10:43:44 -0500
4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 26 -- Fri, 20 Nov 2009 10:43:43 -0500
4, 26 -- Mime-Version: 1.0
4, 26 -- Message-Id: {f06240804c72c66f16bf3-at-[141.209.160.249]}
4, 26 -- In-Reply-To: {200911201451.nAKEpxTW002371-at-ns.microscopy.com}
4, 26 -- References: {200911201451.nAKEpxTW002371-at-ns.microscopy.com}
4, 26 -- Date: Fri, 20 Nov 2009 10:43:42 -0500
4, 26 -- To: kaszas.1-at-osu.edu
4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 26 -- Subject: Re: [Microscopy] viaWWW: Spurr resin
4, 26 -- Cc: Microscopy-at-microscopy.com
4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 26 -- X-OriginalArrivalTime: 20 Nov 2009 15:43:44.0034 (UTC) FILETIME=[3DA26820:01CA69F8]
4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 26 -- X-Spam-Score: -4.40 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 26 -- X-CanItPRO-Stream: default
4, 26 -- X-Canit-Stats-ID: 22850002 - 2933b56b684d - 20091120
4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Fri, 20 Nov 2009 09:52:58 -0600
Subject: [Microscopy] Re: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy

For many years we had one of those amazing EM technicians (largely
self-taught) who was unafraid to tackle any problem with a microscope. About
10 or 11 years ago our EM420 had a leak in the lens cooling circuit, such
that water was dripping inside the column and onto the phosphor viewing
screen! Larry courageously disassembled the entire column, repaired, cleaned
and reassembled it so that it performed very well for 8 or 9 more years.

Larry retired before I came on board so I, to my deep regret, did not have
the opportunity to sit at the feet of this Jedi Master.

Cheers

Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5453
fax: 860-486-4745


} From: {TindallR-at-missouri.edu}
} Reply-To: {TindallR-at-missouri.edu}
} Date: Fri, 20 Nov 2009 09:17:30 -0600
} To: {raristau-at-ims.uconn.edu}
} Subject: [Microscopy] RE: viaWWW: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I don't know about others, but there is absolutely no way I would tackle
} cleaning a TEM column myself. I have done SEM columns in the past, but that's
} a different critter altogether, seems to me.
}
} Are there labs out there that do this in-house without trained service
} engineers? I'm curious.
}
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&N
} avType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} -----Original Message-----
} X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
} Sent: Friday, November 20, 2009 8:47 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: wadowska-at-upei.ca
} Name: Dorota Wadowska
}
} Organization: University of PEI
}
} Title-Subject: [Filtered] TEM how to clean column
}
} Question: Hi guys,
} Thanks for your input about column and TMP. My next question is: how
} to clean a column? Would you do it yourself or leave it to a service
} person?
} Thank you
} Dorota
}
} Login Host: 137.149.102.148
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
} 6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se
} [129.16.200.133])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKEk79r018312
} 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
} 6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM how to clean column
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
} 19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKFEWYj029978
} 19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32 -0600
} 19, 33 -- X-IronPort-Anti-Spam-Filtered: true
} 19, 33 -- X-IronPort-Anti-Spam-Result:
} AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
} 19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009
} 09:14:31 -0600
} 19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
} um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
} 19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} 19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20
} Nov 2009
} 19, 33 -- 09:14:31 -0600
} 19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
} 19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
} 19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
} 19, 33 -- Message-ID:
} {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
} 19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- Accept-Language: en-US
} 19, 33 -- Content-Language: en-US
} 19, 33 -- X-MS-Has-Attach:
} 19, 33 -- X-MS-TNEF-Correlator:
} 19, 33 -- acceptlanguage: en-US
} 19, 33 -- Content-Type: text/plain; charset="us-ascii"
} 19, 33 -- MIME-Version: 1.0
} 19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC)
} FILETIME=[28FBFCA0:01CA69F4]
} 19, 33 -- Content-Transfer-Encoding: 8bit
} 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id nAKFEWYj029978
} ==============================End of - Headers==============================



==============================Original Headers==============================
8, 23 -- From raristau-at-ims.uconn.edu Fri Nov 20 09:52:57 2009
8, 23 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFqvgw010288
8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 09:52:57 -0600
8, 23 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu [137.99.20.157])
8, 23 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id nAKFquX0024504
8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 10:52:56 -0500
8, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122
8, 23 -- Date: Fri, 20 Nov 2009 10:52:55 -0500
8, 23 -- Subject: Re: [Microscopy] TEM how to clean column
8, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
8, 23 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
8, 23 -- Message-ID: {C72C2587.9CB%raristau-at-ims.uconn.edu}
8, 23 -- Thread-Topic: [Microscopy] TEM how to clean column
8, 23 -- Thread-Index: Acpp+YYJxIv4eNXsEd6zNQAbY55CVA==
8, 23 -- In-Reply-To: {200911201517.nAKFHU0t003822-at-ns.microscopy.com}
8, 23 -- Mime-version: 1.0
8, 23 -- Content-type: text/plain;
8, 23 -- charset="US-ASCII"
8, 23 -- Content-transfer-encoding: 7bit
8, 23 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
8, 23 -- X-UConn-MailScanner: Found to be clean
8, 23 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Fri, 20 Nov 2009 09:57:45 -0600
Subject: [Microscopy] Re: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wow. He was more courageous than I would be (unless of course I was under the tutelage of Jedi Master Steve Chapman or similarly talented adept).

I did try to completely disassemble a 1965 Corvair Monza one time, but, well......

Randy

-----Original Message-----
X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu]
Sent: Friday, November 20, 2009 9:54 AM
To: Tindall, Randy D.

Randy

For many years we had one of those amazing EM technicians (largely
self-taught) who was unafraid to tackle any problem with a microscope. About
10 or 11 years ago our EM420 had a leak in the lens cooling circuit, such
that water was dripping inside the column and onto the phosphor viewing
screen! Larry courageously disassembled the entire column, repaired, cleaned
and reassembled it so that it performed very well for 8 or 9 more years.

Larry retired before I came on board so I, to my deep regret, did not have
the opportunity to sit at the feet of this Jedi Master.

Cheers

Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5453
fax: 860-486-4745


} From: {TindallR-at-missouri.edu}
} Reply-To: {TindallR-at-missouri.edu}
} Date: Fri, 20 Nov 2009 09:17:30 -0600
} To: {raristau-at-ims.uconn.edu}
} Subject: [Microscopy] RE: viaWWW: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I don't know about others, but there is absolutely no way I would tackle
} cleaning a TEM column myself. I have done SEM columns in the past, but that's
} a different critter altogether, seems to me.
}
} Are there labs out there that do this in-house without trained service
} engineers? I'm curious.
}
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&N
} avType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} -----Original Message-----
} X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
} Sent: Friday, November 20, 2009 8:47 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: wadowska-at-upei.ca
} Name: Dorota Wadowska
}
} Organization: University of PEI
}
} Title-Subject: [Filtered] TEM how to clean column
}
} Question: Hi guys,
} Thanks for your input about column and TMP. My next question is: how
} to clean a column? Would you do it yourself or leave it to a service
} person?
} Thank you
} Dorota
}
} Login Host: 137.149.102.148
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
} 6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se
} [129.16.200.133])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKEk79r018312
} 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
} 6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM how to clean column
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
} 19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKFEWYj029978
} 19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32 -0600
} 19, 33 -- X-IronPort-Anti-Spam-Filtered: true
} 19, 33 -- X-IronPort-Anti-Spam-Result:
} AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
} 19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009
} 09:14:31 -0600
} 19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
} um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
} 19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} 19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20
} Nov 2009
} 19, 33 -- 09:14:31 -0600
} 19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
} 19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
} 19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
} 19, 33 -- Message-ID:
} {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
} 19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- Accept-Language: en-US
} 19, 33 -- Content-Language: en-US
} 19, 33 -- X-MS-Has-Attach:
} 19, 33 -- X-MS-TNEF-Correlator:
} 19, 33 -- acceptlanguage: en-US
} 19, 33 -- Content-Type: text/plain; charset="us-ascii"
} 19, 33 -- MIME-Version: 1.0
} 19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC)
} FILETIME=[28FBFCA0:01CA69F4]
} 19, 33 -- Content-Transfer-Encoding: 8bit
} 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id nAKFEWYj029978
} ==============================End of - Headers==============================



==============================Original Headers==============================
8, 23 -- From raristau-at-ims.uconn.edu Fri Nov 20 09:52:57 2009
8, 23 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFqvgw010288
8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 09:52:57 -0600
8, 23 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu [137.99.20.157])
8, 23 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id nAKFquX0024504
8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 10:52:56 -0500
8, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122
8, 23 -- Date: Fri, 20 Nov 2009 10:52:55 -0500
8, 23 -- Subject: Re: [Microscopy] TEM how to clean column
8, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
8, 23 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
8, 23 -- Message-ID: {C72C2587.9CB%raristau-at-ims.uconn.edu}
8, 23 -- Thread-Topic: [Microscopy] TEM how to clean column
8, 23 -- Thread-Index: Acpp+YYJxIv4eNXsEd6zNQAbY55CVA==
8, 23 -- In-Reply-To: {200911201517.nAKFHU0t003822-at-ns.microscopy.com}
8, 23 -- Mime-version: 1.0
8, 23 -- Content-type: text/plain;
8, 23 -- charset="US-ASCII"
8, 23 -- Content-transfer-encoding: 7bit
8, 23 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
8, 23 -- X-UConn-MailScanner: Found to be clean
8, 23 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================


==============================Original Headers==============================
17, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:57:45 2009
17, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFvj1m018898
17, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:57:45 -0600
17, 33 -- X-IronPort-Anti-Spam-Filtered: true
17, 33 -- X-IronPort-Anti-Spam-Result: AkoJAN5KBkvRauUo/2dsb2JhbACVEIdeqUcBAQEHhXCIT4JSAQGBaASBb4sT
17, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
17, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009 09:57:44 -0600
17, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
17, 33 -- Fri, 20 Nov 2009 09:57:44 -0600
17, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
17, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20 Nov 2009
17, 33 -- 09:57:43 -0600
17, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
17, 33 -- To: "raristau-at-ims.uconn.edu" {raristau-at-ims.uconn.edu}
17, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
17, 33 -- Date: Fri, 20 Nov 2009 09:57:42 -0600
17, 33 -- Subject: RE: [Microscopy] Re: TEM how to clean column
17, 33 -- Thread-Topic: [Microscopy] Re: TEM how to clean column
17, 33 -- Thread-Index: Acpp+anj/aGQ/uR8S2GGOGq+zjeH0AAADg5g
17, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129B19CACF-at-UM-EMAIL06.um.umsystem.edu}
17, 33 -- References: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
17, 33 -- In-Reply-To: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
17, 33 -- Accept-Language: en-US
17, 33 -- Content-Language: en-US
17, 33 -- X-MS-Has-Attach:
17, 33 -- X-MS-TNEF-Correlator:
17, 33 -- acceptlanguage: en-US
17, 33 -- Content-Type: text/plain; charset="us-ascii"
17, 33 -- MIME-Version: 1.0
17, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:57:44.0497 (UTC) FILETIME=[3296E210:01CA69FA]
17, 33 -- Content-Transfer-Encoding: 8bit
17, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAKFvj1m018898
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Fri, 20 Nov 2009 10:09:05 -0600
Subject: [Microscopy] Re: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy

Yes, but like the surgeon taking the automotive course, did you do the
Corvair through the exhaust pipe? ;-)

paul

--
Paul R. Hazelton, PhD
Viral Gastroenteritis Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
745 William Avenue
Winnipeg, Manitoba, Canada, R3E 0J9
e-mail: paul_hazelton-at-umanitoba.ca
paulhazelton-at-mts.net
Phone: 204-789-3313 (w);
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926



==============================Original Headers==============================
6, 21 -- From paul_hazelton-at-umanitoba.ca Fri Nov 20 10:09:05 2009
6, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKG9552007256
6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 10:09:05 -0600
6, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69])
6, 21 -- (authenticated bits=0)
6, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id nAKG92Gd006010;
6, 21 -- Fri, 20 Nov 2009 10:09:02 -0600 (CST)
6, 21 -- Message-ID: {4B06BF1C.9030902-at-umanitoba.ca}
6, 21 -- Date: Fri, 20 Nov 2009 10:09:00 -0600
6, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
6, 21 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
6, 21 -- MIME-Version: 1.0
6, 21 -- To: TindallR-at-missouri.edu
6, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
6, 21 -- Subject: Re: [Microscopy] TEM how to clean column
6, 21 -- References: {200911201559.nAKFxHKt023620-at-ns.microscopy.com}
6, 21 -- In-Reply-To: {200911201559.nAKFxHKt023620-at-ns.microscopy.com}
6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 21 -- Content-Transfer-Encoding: 7bit
6, 21 -- X-DCC-UofM-Metrics: electra; whitelist
==============================End of - Headers==============================




From: vapatpxs-at-yahoo.com
Date: Fri, 20 Nov 2009 10:57:25 -0600
Subject: [Microscopy] 1 micron thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

Time is short (I'm leaving here and going to Duke, Hi Andy!) and I have to stain over 100 slides that have 1 micron thick sections on them (10 sections per slide). T. blue is out since they want pretty colors like pink and purple. Paragaon didn't work, though on 0.5 micron LR White sections it is very pretty.

Next I'm going to try Richardson's--does anyone have experience using Richardson's on 1 micron thick epon sections.

This lab uses SciPoxy 812 and it seems to be a bit different from other 812 substitutes I've used in the past.

Help!!!

Tick-tock,

Paula :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


C-MI for your imaging needs.





==============================Original Headers==============================
13, 20 -- From vapatpxs-at-yahoo.com Fri Nov 20 10:57:25 2009
13, 20 -- Received: from web46106.mail.sp1.yahoo.com (web46106.mail.sp1.yahoo.com [68.180.199.123])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAKGvOrN023712
13, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 10:57:25 -0600
13, 20 -- Received: (qmail 27390 invoked by uid 60001); 20 Nov 2009 16:57:24 -0000
13, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1258736244; bh=TmFqvLiEwg51Doz9fE4LSPF+bMM4SyPHrDqgtmYhneQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=OhByowxp+z/Fjw4NiH58xpUlIVZZH5ZNN1X99ekd9PsLarv3z3EoTpWrkcsotyI1FWJq3G4BeqZRtQoAbF01Y8X4d0LDevWzsyuPerTevBfUcHHpncNeyCyLpi0DI+JCdYV1jrmmOJefltnjqXN790J+95eoCaBktrmXudwEbdk=
13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 20 -- s=s1024; d=yahoo.com;
13, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type;
13, 20 -- b=Xhr8cIIGL6OjOU1jR374hg12ZcwzxsRkC4QbfwDFxKBpn2tBHx6i3rt0jfYHLbDcGo9X+C9p5KFUTWvovuh7JYe+fHiklzogWPuY3RK1MvnUXvb1T8dIAF9ZfYBQDjRfkT4DecpIp5c1JP7VMETs4D/Y2EkFmPhnwp2JUe3ydiE=;
13, 20 -- Message-ID: {487495.27032.qm-at-web46106.mail.sp1.yahoo.com}
13, 20 -- X-YMail-OSG: zj3pTZUVM1mruJUVnpyINJ1Ls4gu99RTkUxBsur9gAYKF4A_PetXbT5MfVuljwnpqsecjY5NlWz5OBok4hWhFRqOjVj_01BjHWRMIR5S5eaVBcZEye5V2QE6OjmjyqYW_7PTFljMN7GATtvZn1NnZGaWQ4e51fl5w3cHOuVdMdeZjMaQ2qekTemqq8m1NJkOQVzCvMPBVTdyziB1RDff7kFLelRSJrJqY91ab0jkiFk5_Lv9ponf4UuqLEbTX7e9vfo-
13, 20 -- Received: from [132.239.85.200] by web46106.mail.sp1.yahoo.com via HTTP; Fri, 20 Nov 2009 08:57:24 PST
13, 20 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.8.100.260964
13, 20 -- Date: Fri, 20 Nov 2009 08:57:24 -0800 (PST)
13, 20 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com}
13, 20 -- Subject: 1 micron thick sections
13, 20 -- To: MSA BB {Microscopy-at-Microscopy.Com}
13, 20 -- MIME-Version: 1.0
13, 20 -- Content-Type: text/plain; charset=us-ascii
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 20 Nov 2009 13:20:13 -0600
Subject: [Microscopy] Re: TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gosh, you all are wimps! I have taken apart and cleaned the comumns of a
Philips 201, Philips 300, Hitachi H-600, Zeiss 10/A and a little of a
Zeiss 912. Yes, except for the last, they are all really old scopes, and
quite amenable to being dissassembled.

The person asking the question did not state what kind of TEM, so it
really depends. My Zeiss 10 manual is very detailed about cleaning the
column, and easy to follow. The others were more seat-of-the-pants. But a
TEM is easy; it's a bunch of coils stacked up on each other with a clenaup
tube that comes apart in sections for cleaning. Some fixed apertures that
may be interesting to try to remove and clean. If it's an older, not
overly-digital-sensorized instrument and they have some intelligent help,
it's doable. But if they have the money, hire a professional! With new
o-rings.

On the other hand, I would never take apart my SEM! OK, wait, I've done
that, too, but not to put back together!

I used to rebuild VW engines. On the ground, not through the tailpipe.
When you live in the middle of the Pacific ocean, you learn to become
self-sufficient.

Aloha, Tina

} Wow. He was more courageous than I would be (unless of course I was
} under the tutelage of Jedi Master Steve Chapman or similarly talented
} adept).
}
} I did try to completely disassemble a 1965 Corvair Monza one time, but, well......
}
} Randy
}
} -----Original Message-----
} X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu]
} Sent: Friday, November 20, 2009 9:54 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Re: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Randy
}
} For many years we had one of those amazing EM technicians (largely
} self-taught) who was unafraid to tackle any problem with a microscope. About
} 10 or 11 years ago our EM420 had a leak in the lens cooling circuit, such
} that water was dripping inside the column and onto the phosphor viewing
} screen! Larry courageously disassembled the entire column, repaired, cleaned
} and reassembled it so that it performed very well for 8 or 9 more years.
}
} Larry retired before I came on board so I, to my deep regret, did not have
} the opportunity to sit at the feet of this Jedi Master.
}
} Cheers
}
} Roger A. Ristau, PhD
} Electron Microscopy Specialist
} Institute of Materials Science
} 97 North Eagleville Road
} University of Connecticut
} Storrs, CT 06269
} vox: 860-486-5453
} fax: 860-486-4745
}
}
} } From: {TindallR-at-missouri.edu}
} } Reply-To: {TindallR-at-missouri.edu}
} } Date: Fri, 20 Nov 2009 09:17:30 -0600
} } To: {raristau-at-ims.uconn.edu}
} } Subject: [Microscopy] RE: viaWWW: TEM how to clean column
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I don't know about others, but there is absolutely no way I would tackle
} } cleaning a TEM column myself. I have done SEM columns in the past, but that's
} } a different critter altogether, seems to me.
} }
} } Are there labs out there that do this in-house without trained service
} } engineers? I'm curious.
} }
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&N
} } avType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
} } Sent: Friday, November 20, 2009 8:47 AM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: TEM how to clean column
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} } ---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when replying
} } please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
} } ---------------------------------------------------------------------------
} }
} } Email: wadowska-at-upei.ca
} } Name: Dorota Wadowska
} }
} } Organization: University of PEI
} }
} } Title-Subject: [Filtered] TEM how to clean column
} }
} } Question: Hi guys,
} } Thanks for your input about column and TMP. My next question is: how
} } to clean a column? Would you do it yourself or leave it to a service
} } person?
} } Thank you
} } Dorota
} }
} } Login Host: 137.149.102.148
} } ---------------------------------------------------------------------------
} }
} } ==============================Original Headers==============================
} } 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
} } 6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se
} } [129.16.200.133])
} } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } nAKEk79r018312
} } 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
} } 6, 11 -- Mime-Version: 1.0
} } 6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
} } 6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
} } 6, 11 -- To: microscopy-at-microscopy.com
} } 6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
} } 6, 11 -- Subject: viaWWW: TEM how to clean column
} } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } ==============================End of - Headers==============================
} }
} }
} } ==============================Original Headers==============================
} } 19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
} } 19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} } 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } nAKFEWYj029978
} } 19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32 -0600
} } 19, 33 -- X-IronPort-Anti-Spam-Filtered: true
} } 19, 33 -- X-IronPort-Anti-Spam-Result:
} } AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
} } 19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} } ([209.106.229.40])
} } 19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009
} } 09:14:31 -0600
} } 19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
} } um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } 19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
} } 19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} } 19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20
} } Nov 2009
} } 19, 33 -- 09:14:31 -0600
} } 19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} } 19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
} } 19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } 19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
} } 19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
} } 19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
} } 19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
} } 19, 33 -- Message-ID:
} } {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
} } 19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} } 19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} } 19, 33 -- Accept-Language: en-US
} } 19, 33 -- Content-Language: en-US
} } 19, 33 -- X-MS-Has-Attach:
} } 19, 33 -- X-MS-TNEF-Correlator:
} } 19, 33 -- acceptlanguage: en-US
} } 19, 33 -- Content-Type: text/plain; charset="us-ascii"
} } 19, 33 -- MIME-Version: 1.0
} } 19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC)
} } FILETIME=[28FBFCA0:01CA69F4]
} } 19, 33 -- Content-Transfer-Encoding: 8bit
} } 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id nAKFEWYj029978
} } ==============================End of - Headers==============================
}
}
}
} ==============================Original Headers==============================
} 8, 23 -- From raristau-at-ims.uconn.edu Fri Nov 20 09:52:57 2009
} 8, 23 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
} 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFqvgw010288
} 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 09:52:57 -0600
} 8, 23 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu [137.99.20.157])
} 8, 23 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id nAKFquX0024504
} 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 10:52:56 -0500
} 8, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122
} 8, 23 -- Date: Fri, 20 Nov 2009 10:52:55 -0500
} 8, 23 -- Subject: Re: [Microscopy] TEM how to clean column
} 8, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
} 8, 23 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
} 8, 23 -- Message-ID: {C72C2587.9CB%raristau-at-ims.uconn.edu}
} 8, 23 -- Thread-Topic: [Microscopy] TEM how to clean column
} 8, 23 -- Thread-Index: Acpp+YYJxIv4eNXsEd6zNQAbY55CVA==
} 8, 23 -- In-Reply-To: {200911201517.nAKFHU0t003822-at-ns.microscopy.com}
} 8, 23 -- Mime-version: 1.0
} 8, 23 -- Content-type: text/plain;
} 8, 23 -- charset="US-ASCII"
} 8, 23 -- Content-transfer-encoding: 7bit
} 8, 23 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
} 8, 23 -- X-UConn-MailScanner: Found to be clean
} 8, 23 -- X-UConn-MailScanner-SpamCheck:
} ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 17, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:57:45 2009
} 17, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKFvj1m018898
} 17, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:57:45 -0600
} 17, 33 -- X-IronPort-Anti-Spam-Filtered: true
} 17, 33 -- X-IronPort-Anti-Spam-Result: AkoJAN5KBkvRauUo/2dsb2JhbACVEIdeqUcBAQEHhXCIT4JSAQGBaASBb4sT
} 17, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
} 17, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009 09:57:44 -0600
} 17, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 17, 33 -- Fri, 20 Nov 2009 09:57:44 -0600
} 17, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} 17, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20 Nov 2009
} 17, 33 -- 09:57:43 -0600
} 17, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 17, 33 -- To: "raristau-at-ims.uconn.edu" {raristau-at-ims.uconn.edu}
} 17, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 17, 33 -- Date: Fri, 20 Nov 2009 09:57:42 -0600
} 17, 33 -- Subject: RE: [Microscopy] Re: TEM how to clean column
} 17, 33 -- Thread-Topic: [Microscopy] Re: TEM how to clean column
} 17, 33 -- Thread-Index: Acpp+anj/aGQ/uR8S2GGOGq+zjeH0AAADg5g
} 17, 33 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129B19CACF-at-UM-EMAIL06.um.umsystem.edu}
} 17, 33 -- References: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
} 17, 33 -- In-Reply-To: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
} 17, 33 -- Accept-Language: en-US
} 17, 33 -- Content-Language: en-US
} 17, 33 -- X-MS-Has-Attach:
} 17, 33 -- X-MS-TNEF-Correlator:
} 17, 33 -- acceptlanguage: en-US
} 17, 33 -- Content-Type: text/plain; charset="us-ascii"
} 17, 33 -- MIME-Version: 1.0
} 17, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:57:44.0497 (UTC) FILETIME=[3296E210:01CA69FA]
} 17, 33 -- Content-Transfer-Encoding: 8bit
} 17, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAKFvj1m018898
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
8, 20 -- From tina-at-pbrc.hawaii.edu Fri Nov 20 11:56:45 2009
8, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKHuine008105
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 11:56:45 -0600
8, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id nAKHufr5006828
8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 07:56:42 -1000 (HST)
8, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id nAKHufII006825
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 07:56:41 -1000 (HST)
8, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
8, 20 -- Date: Fri, 20 Nov 2009 07:56:40 -1000 (HST)
8, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 20 -- X-Sender: tina-at-halia
8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 20 -- Subject: Re: [Microscopy] TEM how to clean column
8, 20 -- In-Reply-To: {200911201558.nAKFwY9S021265-at-ns.microscopy.com}
8, 20 -- Message-ID: {Pine.GSO.4.21.0911200747010.6757-100000-at-halia}
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================

From quotno-at-migi.com Fri Nov 20 12:22:43 2009
Return-Path: {quotno-at-migi.com}
Received: from ordenar (cm-93-156-243-123.telecable.es [93.156.243.123])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKIMdjx023295;
Fri, 20 Nov 2009 12:22:41 -0600
Received: from [93.156.243.123] by server85.appriver.com; Fri, 20 Nov 2009 19:20:12 +0100

Go Tina! BTW, SEM columns are generally simpler than TEM columns (and even
VW engines) so have at it!
Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu]
Sent: Friday, November 20, 2009 12:59 PM
To: kenconverse-at-qualityimages.biz

Gosh, you all are wimps! I have taken apart and cleaned the comumns of a
Philips 201, Philips 300, Hitachi H-600, Zeiss 10/A and a little of a
Zeiss 912. Yes, except for the last, they are all really old scopes, and
quite amenable to being dissassembled.

The person asking the question did not state what kind of TEM, so it
really depends. My Zeiss 10 manual is very detailed about cleaning the
column, and easy to follow. The others were more seat-of-the-pants. But a
TEM is easy; it's a bunch of coils stacked up on each other with a clenaup
tube that comes apart in sections for cleaning. Some fixed apertures that
may be interesting to try to remove and clean. If it's an older, not
overly-digital-sensorized instrument and they have some intelligent help,
it's doable. But if they have the money, hire a professional! With new
o-rings.

On the other hand, I would never take apart my SEM! OK, wait, I've done
that, too, but not to put back together!

I used to rebuild VW engines. On the ground, not through the tailpipe.
When you live in the middle of the Pacific ocean, you learn to become
self-sufficient.

Aloha, Tina

} Wow. He was more courageous than I would be (unless of course I was
} under the tutelage of Jedi Master Steve Chapman or similarly talented
} adept).
}
} I did try to completely disassemble a 1965 Corvair Monza one time, but,
well......
}
} Randy
}
} -----Original Message-----
} X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu]
} Sent: Friday, November 20, 2009 9:54 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Re: TEM how to clean column
}
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Randy
}
} For many years we had one of those amazing EM technicians (largely
} self-taught) who was unafraid to tackle any problem with a microscope.
About
} 10 or 11 years ago our EM420 had a leak in the lens cooling circuit, such
} that water was dripping inside the column and onto the phosphor viewing
} screen! Larry courageously disassembled the entire column, repaired,
cleaned
} and reassembled it so that it performed very well for 8 or 9 more years.
}
} Larry retired before I came on board so I, to my deep regret, did not have
} the opportunity to sit at the feet of this Jedi Master.
}
} Cheers
}
} Roger A. Ristau, PhD
} Electron Microscopy Specialist
} Institute of Materials Science
} 97 North Eagleville Road
} University of Connecticut
} Storrs, CT 06269
} vox: 860-486-5453
} fax: 860-486-4745
}
}
} } From: {TindallR-at-missouri.edu}
} } Reply-To: {TindallR-at-missouri.edu}
} } Date: Fri, 20 Nov 2009 09:17:30 -0600
} } To: {raristau-at-ims.uconn.edu}
} } Subject: [Microscopy] RE: viaWWW: TEM how to clean column
} }
} }
} }
} }
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } I don't know about others, but there is absolutely no way I would tackle
} } cleaning a TEM column myself. I have done SEM columns in the past, but
that's
} } a different critter altogether, seems to me.
} }
} } Are there labs out there that do this in-house without trained service
} } engineers? I'm curious.
} }
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} }
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week
&N
} } avType=Both&Type=TimePlan
} } Sons of Norway: http://www.sofn.com
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
} } Sent: Friday, November 20, 2009 8:47 AM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: TEM how to clean column
} }
} }
} }
} }
} }
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} }
---------------------------------------------------------------------------
} } Remember this posting is most likely not from a Subscriber, so when
replying
} } please copy both wadowska-at-upei.ca as well as the MIcroscopy
Listserver
} }
---------------------------------------------------------------------------
} }
} } Email: wadowska-at-upei.ca
} } Name: Dorota Wadowska
} }
} } Organization: University of PEI
} }
} } Title-Subject: [Filtered] TEM how to clean column
} }
} } Question: Hi guys,
} } Thanks for your input about column and TMP. My next question is: how
} } to clean a column? Would you do it yourself or leave it to a service
} } person?
} } Thank you
} } Dorota
} }
} } Login Host: 137.149.102.148
} }
---------------------------------------------------------------------------
} }
} } ==============================Original
Headers==============================
} } 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
} } 6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se
} } [129.16.200.133])
} } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } nAKEk79r018312
} } 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08
-0600
} } 6, 11 -- Mime-Version: 1.0
} } 6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
} } 6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
} } 6, 11 -- To: microscopy-at-microscopy.com
} } 6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
} } 6, 11 -- Subject: viaWWW: TEM how to clean column
} } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} } ==============================End of -
Headers==============================
} }
} }
} } ==============================Original
Headers==============================
} } 19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
} } 19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} } 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} } nAKFEWYj029978
} } 19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32
-0600
} } 19, 33 -- X-IronPort-Anti-Spam-Filtered: true
} } 19, 33 -- X-IronPort-Anti-Spam-Result:
} } AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
} } 19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} } ([209.106.229.40])
} } 19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov
2009
} } 09:14:31 -0600
} } 19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181])
by
} } um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} } 19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
} } 19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} } 19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri,
20
} } Nov 2009
} } 19, 33 -- 09:14:31 -0600
} } 19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} } 19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
} } 19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} } 19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
} } 19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
} } 19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
} } 19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
} } 19, 33 -- Message-ID:
} } {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
} } 19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} } 19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} } 19, 33 -- Accept-Language: en-US
} } 19, 33 -- Content-Language: en-US
} } 19, 33 -- X-MS-Has-Attach:
} } 19, 33 -- X-MS-TNEF-Correlator:
} } 19, 33 -- acceptlanguage: en-US
} } 19, 33 -- Content-Type: text/plain; charset="us-ascii"
} } 19, 33 -- MIME-Version: 1.0
} } 19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC)
} } FILETIME=[28FBFCA0:01CA69F4]
} } 19, 33 -- Content-Transfer-Encoding: 8bit
} } 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id nAKFEWYj029978
} } ==============================End of -
Headers==============================
}
}
}
} ==============================Original
Headers==============================
} 8, 23 -- From raristau-at-ims.uconn.edu Fri Nov 20 09:52:57 2009
} 8, 23 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu
[137.99.25.204])
} 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAKFqvgw010288
} 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 09:52:57
-0600
} 8, 23 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu
[137.99.20.157])
} 8, 23 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id
nAKFquX0024504
} 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Nov 2009 10:52:56
-0500
} 8, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122
} 8, 23 -- Date: Fri, 20 Nov 2009 10:52:55 -0500
} 8, 23 -- Subject: Re: [Microscopy] TEM how to clean column
} 8, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
} 8, 23 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
} 8, 23 -- Message-ID: {C72C2587.9CB%raristau-at-ims.uconn.edu}
} 8, 23 -- Thread-Topic: [Microscopy] TEM how to clean column
} 8, 23 -- Thread-Index: Acpp+YYJxIv4eNXsEd6zNQAbY55CVA==
} 8, 23 -- In-Reply-To: {200911201517.nAKFHU0t003822-at-ns.microscopy.com}
} 8, 23 -- Mime-version: 1.0
} 8, 23 -- Content-type: text/plain;
} 8, 23 -- charset="US-ASCII"
} 8, 23 -- Content-transfer-encoding: 7bit
} 8, 23 -- X-UConn-MailScanner-Information: Contact UConn Help Desk
860-486-4357 for more information.
} 8, 23 -- X-UConn-MailScanner: Found to be clean
} 8, 23 -- X-UConn-MailScanner-SpamCheck:
} ==============================End of -
Headers==============================
}
}
} ==============================Original
Headers==============================
} 17, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:57:45 2009
} 17, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
(mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAKFvj1m018898
} 17, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:57:45
-0600
} 17, 33 -- X-IronPort-Anti-Spam-Filtered: true
} 17, 33 -- X-IronPort-Anti-Spam-Result:
AkoJAN5KBkvRauUo/2dsb2JhbACVEIdeqUcBAQEHhXCIT4JSAQGBaASBb4sT
} 17, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
([209.106.229.40])
} 17, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov
2009 09:57:44 -0600
} 17, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 17, 33 -- Fri, 20 Nov 2009 09:57:44 -0600
} 17, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} 17, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri,
20 Nov 2009
} 17, 33 -- 09:57:43 -0600
} 17, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 17, 33 -- To: "raristau-at-ims.uconn.edu" {raristau-at-ims.uconn.edu}
} 17, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 17, 33 -- Date: Fri, 20 Nov 2009 09:57:42 -0600
} 17, 33 -- Subject: RE: [Microscopy] Re: TEM how to clean column
} 17, 33 -- Thread-Topic: [Microscopy] Re: TEM how to clean column
} 17, 33 -- Thread-Index: Acpp+anj/aGQ/uR8S2GGOGq+zjeH0AAADg5g
} 17, 33 -- Message-ID:
{9422E68616A7C648A281C0B5CD22A4B8129B19CACF-at-UM-EMAIL06.um.umsystem.edu}
} 17, 33 -- References: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
} 17, 33 -- In-Reply-To: {200911201553.nAKFrpuA011804-at-ns.microscopy.com}
} 17, 33 -- Accept-Language: en-US
} 17, 33 -- Content-Language: en-US
} 17, 33 -- X-MS-Has-Attach:
} 17, 33 -- X-MS-TNEF-Correlator:
} 17, 33 -- acceptlanguage: en-US
} 17, 33 -- Content-Type: text/plain; charset="us-ascii"
} 17, 33 -- MIME-Version: 1.0
} 17, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:57:44.0497 (UTC)
FILETIME=[3296E210:01CA69FA]
} 17, 33 -- Content-Transfer-Encoding: 8bit
} 17, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nAKFvj1m018898
} ==============================End of -
Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *

* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*

****************************************************************************


==============================Original Headers==============================
8, 20 -- From tina-at-pbrc.hawaii.edu Fri Nov 20 11:56:45 2009
8, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu
[128.171.22.7])
8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAKHuine008105
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 11:56:45
-0600
8, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id
nAKHufr5006828
8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
verify=NO)
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 07:56:42
-1000 (HST)
8, 20 -- Received: from localhost by halia.pbrc.hawaii.edu
(8.12.11/8.12.11/Submit) with ESMTP id nAKHufII006825
8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 07:56:41
-1000 (HST)
8, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process
doing -bs
8, 20 -- Date: Fri, 20 Nov 2009 07:56:40 -1000 (HST)
8, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 20 -- X-Sender: tina-at-halia
8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 20 -- Subject: Re: [Microscopy] TEM how to clean column
8, 20 -- In-Reply-To: {200911201558.nAKFwY9S021265-at-ns.microscopy.com}
8, 20 -- Message-ID: {Pine.GSO.4.21.0911200747010.6757-100000-at-halia}
8, 20 -- MIME-Version: 1.0
8, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




==============================Original Headers==============================
21, 25 -- From kenconverse-at-qualityimages.biz Fri Nov 20 13:20:13 2009
21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121])
21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKJKC72025276
21, 25 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:20:12 -0600
21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com
21, 25 -- with ESMTP
21, 25 -- id {20091120192010714.XXHI13252-at-cdptpa-omta04.mail.rr.com} ;
21, 25 -- Fri, 20 Nov 2009 19:20:10 +0000
21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
21, 25 -- To: {tina-at-pbrc.hawaii.edu} , "MSA Listserver" {microscopy-at-microscopy.com}
21, 25 -- Subject: RE: [Microscopy] Re: TEM how to clean column
21, 25 -- Date: Fri, 20 Nov 2009 14:20:00 -0500
21, 25 -- Message-ID: {CDCF5B45412E4408ADB1BB9C29799398-at-Ken}
21, 25 -- MIME-Version: 1.0
21, 25 -- Content-Type: text/plain;
21, 25 -- charset="us-ascii"
21, 25 -- X-Priority: 3 (Normal)
21, 25 -- X-MSMail-Priority: Normal
21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838
21, 25 -- Importance: Normal
21, 25 -- Thread-Index: AcpqCy3bCCXt+0KWSta7dSSZ5piFegACvixg
21, 25 -- In-Reply-To: {200911201759.nAKHxHv3011898-at-ns.microscopy.com}
21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
21, 25 -- Content-Transfer-Encoding: 8bit
21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAKJKC72025276
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Fri, 20 Nov 2009 13:44:06 -0600
Subject: [Microscopy] TEM how to clean column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Go Tina! BTW, SEM columns are generally simpler than TEM columns (and
even
} VW engines) so have at it!
} Ken Converse

Ah, but I have a Hitachi S-4800 cold cathode field emitter with all kinds
of detectors that I'm a little afraid of. Although I did help install it
plus another one the very next day, when Hitachi sent out their female
engineer (we girls scared the boys), so I'm pretty comfortable with
it. Here's what I did to our old S-800
http://www.flickr.com/photos/koolau_arts/3246764752/in/set-72157613221785575/
I keep parts around to show people as I train them.

Alas, whoever will eventually replace me will probably not ever have had
to take apart and repair this kind of equipment. Maybe they shouldn't have
to. It's like the new cars with "black boxes". Although there is hope: I
talked a kid through rebuilding a broken Penning gauge a couple of weeks
ago, and the SEM got pumped down and he's forging ahead on getting it
going.

I took my car in for service yesterday - I can't even tell you what's
under the hood. (OK, that's not true- it's a kind of performance vehicle
and I pretty much understand the specs, but I would never try to even
stick my hand in there.)

Are you "old guys" training up the youngsters? Oh, shoot, I remember being
the youngest person in the place...

Aloha,
Tina




==============================Original Headers==============================
9, 21 -- From tina-at-pbrc.hawaii.edu Fri Nov 20 13:44:05 2009
9, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKJi5IS008021
9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:44:05 -0600
9, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
9, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id nAKJi2ci007167
9, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
9, 21 -- Fri, 20 Nov 2009 09:44:03 -1000 (HST)
9, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id nAKJi1Ec007164;
9, 21 -- Fri, 20 Nov 2009 09:44:02 -1000 (HST)
9, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
9, 21 -- Date: Fri, 20 Nov 2009 09:44:01 -1000 (HST)
9, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
9, 21 -- X-Sender: tina-at-halia
9, 21 -- To: Ken Converse {kenconverse-at-qualityimages.biz}
9, 21 -- cc: MSA Listserver {microscopy-at-microscopy.com}
9, 21 -- Subject: RE: [Microscopy] Re: TEM how to clean column
9, 21 -- In-Reply-To: {CDCF5B45412E4408ADB1BB9C29799398-at-Ken}
9, 21 -- Message-ID: {Pine.GSO.4.21.0911200931040.7068-100000-at-halia}
9, 21 -- MIME-Version: 1.0
9, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: Fogellh-at-rlasd.k12.pa.us
Date: Fri, 20 Nov 2009 13:54:32 -0600
Subject: [Microscopy] viaWWW: SEM Biology at Red Lion Senior High School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Fogellh-at-rlasd.k12.pa.us as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Fogellh-at-rlasd.k12.pa.us
Name: Heather Fogell

Organization: Red Lion Senior High School

Title-Subject: [Filtered] Biology

Question: Our High school in South Central PA is fortunate enough to
have an ETEC SEM and are looking to further our students' scientific
process experience through the formation of a partnership with a
college or university. We would offer use of our microscope and our
students' limited technical services in exchange for the opportunity
for the students to experience the research process driving the use
of the equipment. If interested please contact Heather Fogell at
Fogellh-at-rlasd.k12.pa.us.

Login Host: 208.67.142.65
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 13:54:32 2009
6, 11 -- Received: from [129.16.199.75] (dhcp-199-75.nomad.chalmers.se [129.16.199.75])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKJsUjM022785
6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:54:31 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c72ca44ca312-at-[129.16.200.133]}
6, 11 -- Date: Fri, 20 Nov 2009 20:54:28 +0100
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: Fogellh-at-rlasd.k12.pa.us (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: SEM Biology at Red Lion Senior High School
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: kaszas.1-at-osu.edu
Date: Fri, 20 Nov 2009 13:54:55 -0600
Subject: [Microscopy] viaWWW: Spurr resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kaszas.1-at-osu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: kaszas.1-at-osu.edu
Name: Andrea Kaszas

Organization: Ohio State University/OARDC

Title-Subject: [Filtered] Spurr resin

Question: Dear list members,


Does anybody among you use Spurr resin for studying plant materials
by transmisson electron microscopy?
If so, what protocol do you use with the new formulation of ERL 4221.
Has anyone found another resin for plant tissue for studying cell
structure with transmission electron microscopy?
Any suggestions are greatly appreciated.

Andrea Kaszas
Research assistant



Login Host: 164.107.85.175
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 13:54:55 2009
11, 11 -- Received: from [129.16.199.75] (dhcp-199-75.nomad.chalmers.se [129.16.199.75])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKJsrpY023494
11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:54:54 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c72ca47cae50-at-[129.16.199.75]}
11, 11 -- Date: Fri, 20 Nov 2009 20:54:51 +0100
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: kaszas.1-at-osu.edu (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Spurr resin
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 20 Nov 2009 15:07:43 -0600
Subject: [Microscopy] RE: how not to clean an EM column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Maybe this is an urban legend, there's the story of the faculty member who
assigned the cleaning of an ISI tabletop SEM to his graduate student. He was
leaving for Europe and wouldn't be able to do the cleaning herself. He
simply told the student that the solvents, wipes and Q-tips were in a locker
near the instrument. He wrongly assumed that the student had seen or
assisted others in the process.

The student carefully removed the gun from the top of the column, placed a
glass beaker in the specimen chamber and gently poured acetone down the
column!

John Bozzola
IMAGE Center
Southern Illinois University
Carbondale, IL

==============================Original Headers==============================
3, 15 -- From bozzola-at-siu.edu Fri Nov 20 15:07:43 2009
3, 15 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
3, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKL7gdc021527
3, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 15:07:43 -0600
3, 15 -- Received: by pzk37 with SMTP id 37so2480121pzk.10
3, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:07:42 -0800 (PST)
3, 15 -- MIME-Version: 1.0
3, 15 -- Received: by 10.115.113.6 with SMTP id q6mr2419028wam.55.1258751262398; Fri,
3, 15 -- 20 Nov 2009 13:07:42 -0800 (PST)
3, 15 -- Date: Fri, 20 Nov 2009 15:07:42 -0600
3, 15 -- Message-ID: {ebc2299e0911201307l75fe4a27nfe2230e86136bc10-at-mail.gmail.com}
3, 15 -- Subject: RE: how not to clean an EM column
3, 15 -- From: John Bozzola {bozzola-at-siu.edu}
3, 15 -- To: MSAListserver {Microscopy-at-microscopy.com}
3, 15 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Fri, 20 Nov 2009 15:22:52 -0600
Subject: [Microscopy] how not to clean an EM column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, JOhn-

Probably not urban legend. Here's a version I can absolutely confirm
(although I didn't do it, but arrived only minutes after it happened).

Technician cleaned the TEM column parts with acetone and reassembeled, not
completely dry. Noticed a drop of acetone had fallen on the fluorescent
screen. Removed the window and blew on it with canned air.

The entire room fluoresced for a couple of years...

Aloha,
Tina

} Maybe this is an urban legend, there's the story of the faculty member who
} assigned the cleaning of an ISI tabletop SEM to his graduate student. He was
} leaving for Europe and wouldn't be able to do the cleaning herself. He
} simply told the student that the solvents, wipes and Q-tips were in a locker
} near the instrument. He wrongly assumed that the student had seen or
} assisted others in the process.
}
} The student carefully removed the gun from the top of the column, placed a
} glass beaker in the specimen chamber and gently poured acetone down the
} column!
}
} John Bozzola
} IMAGE Center
} Southern Illinois University
} Carbondale, IL
}
} ==============================Original Headers==============================
} 3, 15 -- From bozzola-at-siu.edu Fri Nov 20 15:07:43 2009
} 3, 15 -- Received: from mail-pz0-f199.google.com (mail-pz0-f199.google.com [209.85.222.199])
} 3, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKL7gdc021527
} 3, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 15:07:43 -0600
} 3, 15 -- Received: by pzk37 with SMTP id 37so2480121pzk.10
} 3, 15 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:07:42 -0800 (PST)
} 3, 15 -- MIME-Version: 1.0
} 3, 15 -- Received: by 10.115.113.6 with SMTP id q6mr2419028wam.55.1258751262398; Fri,
} 3, 15 -- 20 Nov 2009 13:07:42 -0800 (PST)
} 3, 15 -- Date: Fri, 20 Nov 2009 15:07:42 -0600
} 3, 15 -- Message-ID: {ebc2299e0911201307l75fe4a27nfe2230e86136bc10-at-mail.gmail.com}
} 3, 15 -- Subject: RE: how not to clean an EM column
} 3, 15 -- From: John Bozzola {bozzola-at-siu.edu}
} 3, 15 -- To: MSAListserver {Microscopy-at-microscopy.com}
} 3, 15 -- Content-Type: text/plain; charset=UTF-8
} ==============================End of - Headers==============================
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
8, 21 -- From tina-at-pbrc.hawaii.edu Fri Nov 20 15:22:52 2009
8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKLMpXJ006543
8, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 15:22:51 -0600
8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id nAKLMmRL007473
8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
8, 21 -- Fri, 20 Nov 2009 11:22:48 -1000 (HST)
8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id nAKLMl8s007470;
8, 21 -- Fri, 20 Nov 2009 11:22:47 -1000 (HST)
8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
8, 21 -- Date: Fri, 20 Nov 2009 11:22:47 -1000 (HST)
8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
8, 21 -- X-Sender: tina-at-halia
8, 21 -- To: bozzola-at-siu.edu
8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com}
8, 21 -- Subject: Re: [Microscopy] RE: how not to clean an EM column
8, 21 -- In-Reply-To: {200911202108.nAKL8f4S022948-at-ns.microscopy.com}
8, 21 -- Message-ID: {Pine.GSO.4.21.0911201118530.7362-100000-at-halia}
8, 21 -- MIME-Version: 1.0
8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 20 Nov 2009 15:37:45 -0600
Subject: [Microscopy] how not to clean an EM column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here's one that DID happen to me. I was at a faculty meeting (in
Philadelphia), when my graduate student burst into the room shouting
"the electron microscope is on fire!"

I ran over to the room and, sure enough, the serviceman was stamping
his feet on the floor to put out a fire. Here's the story: his method
of cleaning Pt apertures was to flame them and drop them (while hot)
into a beaker of acetone. Normally, this was not a problem as this
would not ignite the acetone. This time, however, his bunsen burner
was too close to the 250 ml beaker and he reported that the flame
appeared to jump from the bunsen over to the beaker, where it went up
quite vigorously.

In his panic to cover the beaker, he tipped it over, sending flaming
acetone along the countertop (all over my NIkon electron flash unit)
and onto the floor. He attempted to stamp out the fire, igniting his
pant legs. The fire was extinguished with CO2 but he had burned his
hands and shin severely enough that he went on medical leave. He never
came back to work. He left permanent footprints on the tile floor and
the EM company never reimbursed us for the damage.

I won't flame the company, by revealing their identity.

John Bozzola
IMAGE Center
Southern Illinois University
Carbondale, IL

==============================Original Headers==============================
5, 18 -- From bozzola-at-siu.edu Fri Nov 20 15:37:45 2009
5, 18 -- Received: from mail-pw0-f51.google.com (mail-pw0-f51.google.com [209.85.160.51])
5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAKLbivm024413
5, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 15:37:45 -0600
5, 18 -- Received: by pwj10 with SMTP id 10so2378559pwj.10
5, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 13:37:44 -0800 (PST)
5, 18 -- MIME-Version: 1.0
5, 18 -- Received: by 10.114.33.30 with SMTP id g30mr2389506wag.214.1258753064339; Fri,
5, 18 -- 20 Nov 2009 13:37:44 -0800 (PST)
5, 18 -- In-Reply-To: {Pine.GSO.4.21.0911201118530.7362-100000-at-halia}
5, 18 -- References: {200911202108.nAKL8f4S022948-at-ns.microscopy.com}
5, 18 -- {Pine.GSO.4.21.0911201118530.7362-100000-at-halia}
5, 18 -- Date: Fri, 20 Nov 2009 15:37:44 -0600
5, 18 -- Message-ID: {ebc2299e0911201337k215acd8cw6f02fe3c444e0402-at-mail.gmail.com}
5, 18 -- Subject: Re: [Microscopy] RE: how not to clean an EM column
5, 18 -- From: John Bozzola {bozzola-at-siu.edu}
5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com}
5, 18 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: wadowska-at-upei.ca
Date: Mon, 23 Nov 2009 08:35:40 -0600
Subject: [Microscopy] viaWWW: TME thank you column cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We used to clean our JEOL 100CX column, gave it a thorough clean just after
I arrived here because oil would condense on the viewing window and had to
be wiped off every month or so..... This was considered normal. Also got
the workshop to take the diff pumps apart and clean out the tarry residue -
what diff pump oil turns into after 20+ years without being changed. The
gun was surprisingly clean - only a faint brown residue in the chamber.
Changed all the vacuum hoses as well to try to stop oil coming out and into
the column. All this improved things a bit - only had to wipe the oil off
the window every 6 months. After a couple of years, it developed an
incurable vacuum sequencing problem (don't get me started), it was just worn
out and oil-soaked, so we retired it (to my great relief, I have to admit).
Not sure I would do this with a new instrument, but we didn't have anything
to lose with the old one.

cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 21/11/09 2:19 AM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I don't know about others, but there is absolutely no way I would tackle
} cleaning a TEM column myself. I have done SEM columns in the past, but that's
} a different critter altogether, seems to me.
}
} Are there labs out there that do this in-house without trained service
} engineers? I'm curious.
}
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&N
} avType=Both&Type=TimePlan
} Sons of Norway: http://www.sofn.com
}
}
}
}
} -----Original Message-----
} X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca]
} Sent: Friday, November 20, 2009 8:47 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: TEM how to clean column
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: wadowska-at-upei.ca
} Name: Dorota Wadowska
}
} Organization: University of PEI
}
} Title-Subject: [Filtered] TEM how to clean column
}
} Question: Hi guys,
} Thanks for your input about column and TMP. My next question is: how
} to clean a column? Would you do it yourself or leave it to a service
} person?
} Thank you
} Dorota
}
} Login Host: 137.149.102.148
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 20 08:46:09 2009
} 6, 11 -- Received: from [129.16.200.133] (dhcp-200-133.nomad.chalmers.se
} [129.16.200.133])
} 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKEk79r018312
} 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 08:46:08 -0600
} 6, 11 -- Mime-Version: 1.0
} 6, 11 -- Message-Id: {p06240801c72c5c1a6290-at-[129.16.200.133]}
} 6, 11 -- Date: Fri, 20 Nov 2009 15:46:05 +0100
} 6, 11 -- To: microscopy-at-microscopy.com
} 6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
} 6, 11 -- Subject: viaWWW: TEM how to clean column
} 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 19, 33 -- From TindallR-at-missouri.edu Fri Nov 20 09:14:32 2009
} 19, 33 -- Received: from mxnip01-missouri-out.um.umsystem.edu
} (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
} 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nAKFEWYj029978
} 19, 33 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 09:14:32 -0600
} 19, 33 -- X-IronPort-Anti-Spam-Filtered: true
} 19, 33 -- X-IronPort-Anti-Spam-Result:
} AkoJAH9BBkvRauUo/2dsb2JhbACVEIdeqTYBAQEHhXGIT4JSAQGBaAQ
} 19, 33 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
} ([209.106.229.40])
} 19, 33 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Nov 2009
} 09:14:31 -0600
} 19, 33 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by
} um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 19, 33 -- Fri, 20 Nov 2009 09:14:31 -0600
} 19, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by
} 19, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 20
} Nov 2009
} 19, 33 -- 09:14:31 -0600
} 19, 33 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} 19, 33 -- To: "wadowska-at-upei.ca" {wadowska-at-upei.ca}
} 19, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 19, 33 -- Date: Fri, 20 Nov 2009 09:14:29 -0600
} 19, 33 -- Subject: RE: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Topic: [Microscopy] viaWWW: TEM how to clean column
} 19, 33 -- Thread-Index: Acpp8GiJmk+SeixzT0m8r58YGHXoRgAAG8Yg
} 19, 33 -- Message-ID:
} {9422E68616A7C648A281C0B5CD22A4B8129B19CAA4-at-UM-EMAIL06.um.umsystem.edu}
} 19, 33 -- References: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- In-Reply-To: {200911201447.nAKElRRK022183-at-ns.microscopy.com}
} 19, 33 -- Accept-Language: en-US
} 19, 33 -- Content-Language: en-US
} 19, 33 -- X-MS-Has-Attach:
} 19, 33 -- X-MS-TNEF-Correlator:
} 19, 33 -- acceptlanguage: en-US
} 19, 33 -- Content-Type: text/plain; charset="us-ascii"
} 19, 33 -- MIME-Version: 1.0
} 19, 33 -- X-OriginalArrivalTime: 20 Nov 2009 15:14:31.0402 (UTC)
} FILETIME=[28FBFCA0:01CA69F4]
} 19, 33 -- Content-Transfer-Encoding: 8bit
} 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id nAKFEWYj029978
} ==============================End of - Headers==============================



==============================Original Headers==============================
9, 44 -- From prvs=569aefc35=Rosemary.White-at-csiro.au Fri Nov 20 20:03:59 2009
9, 44 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37])
9, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAL23wR2016106
9, 44 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2009 20:03:58 -0600
9, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
9, 44 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt;
9, 44 -- s=email; t=1258769039; x=1290305039;
9, 44 -- h=from:sender:reply-to:subject:date:message-id:to:cc:
9, 44 -- mime-version:content-transfer-encoding:content-id:
9, 44 -- content-description:resent-date:resent-from:resent-sender:
9, 44 -- resent-to:resent-cc:resent-message-id:in-reply-to:
9, 44 -- references:list-id:list-help:list-unsubscribe:
9, 44 -- list-subscribe:list-post:list-owner:list-archive;
9, 44 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au}
9, 44 -- |Subject:=20Re:=20[Microscopy]=20RE:=20viaWWW:=20TEM=20ho
9, 44 -- w=20to=20clean=20column|Date:=20Sat,=2021=20Nov=202009=20
9, 44 -- 13:07:49=20+1100|Message-ID:=20 {C72D96A5.160A9%rosemary.w
9, 44 -- hite-at-csiro.au} |To:=20 {microscopy-at-microscopy.com}
9, 44 -- |MIME-Version:=201.0|Content-Transfer-Encoding:=207bit
9, 44 -- |In-Reply-To:=20 {200911201519.nAKFJ4wI006230-at-ns.microscop
9, 44 -- y.com} ;
9, 44 -- bh=yqOSvCsk1pgQ2bB/ZQ/jiJNJCIo+gWPfbQ3kHAY4thU=;
9, 44 -- b=jomTJ9JTk+Qt5kt1vXoXti72QZ0zAZ23UqGfzYGnXjZ0IrCVcAXWTbEk
9, 44 -- TyQZh49yYV+WyqahWz7IT8/BdXyqiZT2zc/FvfH8n/A1ZqSdyvl4VuU3e
9, 44 -- rVzwaQW/zTOjHpI;
9, 44 -- X-IronPort-AV: E=Sophos;i="4.47,262,1257080400";
9, 44 -- d="scan'208";a="27352000"
9, 44 -- Received: from exvic-htca01.nexus.csiro.au ([138.194.81.126])
9, 44 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 21 Nov 2009 13:03:56 +1100
9, 44 -- Received: from [152.83.195.117] (152.83.195.117) by
9, 44 -- exvic-htca01.nexus.csiro.au (138.194.81.126) with Microsoft SMTP Server id
9, 44 -- 8.1.393.1; Sat, 21 Nov 2009 13:03:56 +1100
9, 44 -- User-Agent: Microsoft-Entourage/12.20.0.090605
9, 44 -- Date: Sat, 21 Nov 2009 13:07:49 +1100
9, 44 -- Subject: Re: [Microscopy] RE: viaWWW: TEM how to clean column
9, 44 -- From: Rosemary White {rosemary.white-at-csiro.au}
9, 44 -- To: {microscopy-at-microscopy.com}
9, 44 -- Message-ID: {C72D96A5.160A9%rosemary.white-at-csiro.au}
9, 44 -- Thread-Topic: [Microscopy] RE: viaWWW: TEM how to clean column
9, 44 -- Thread-Index: Acpp9M4b0eVpQNPzR3OQNR8lhipxqQAWp52A
9, 44 -- In-Reply-To: {200911201519.nAKFJ4wI006230-at-ns.microscopy.com}
9, 44 -- MIME-Version: 1.0
9, 44 -- Content-Type: text/plain; charset="US-ASCII"
9, 44 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From quotes-at-discountmovers.com Fri Nov 20 20:48:42 2009
Return-Path: {quotes-at-discountmovers.com}
Received: from home-tyan8cwp84 (212-43-35-152-PPPoE.unitednet.bg [212.43.35.152] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAL2mdhk032068;
Fri, 20 Nov 2009 20:48:41 -0600
Received: from [212.43.35.152] by mail.discountmovers.com; Sat, 21 Nov 2009 04:46:11 +0200

I am sure everyone will be saddened to learn of the passing
of Al Crewe on Wedesday Nov 18. He was an inspiration to all of us
with his creativity and down-to-earth sense of humor. We send
condolences to his family and friends. He will be missed.

} http://www.nytimes.com/2009/11/21/science/21crewe.html


--
Peter Ingram
Sr. Physicist
Adj. Asst. Professor of Pathology
Duke University Medical Center
PO Box 90319
DURHAM NC 27708-0319

Tel: 919 660-2695
Fax: 919 660-2671
Email: p.ingram-at-cellbio.duke.edu

==============================Original Headers==============================
5, 17 -- From p.ingram-at-cellbio.duke.edu Sun Nov 22 10:09:44 2009
5, 17 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123])
5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAMG9iGD008399
5, 17 -- for {microscopy-at-microscopy.com} ; Sun, 22 Nov 2009 10:09:44 -0600
5, 17 -- Received: from [192.168.2.18] (really [98.26.4.162])
5, 17 -- by cdptpa-omta02.mail.rr.com with ESMTP
5, 17 -- id {20091122160944012.KRVJ27026-at-cdptpa-omta02.mail.rr.com}
5, 17 -- for {microscopy-at-microscopy.com} ; Sun, 22 Nov 2009 16:09:44 +0000
5, 17 -- Mime-Version: 1.0
5, 17 -- Message-Id: {a06240800c72f111de6dc-at-[192.168.2.18]}
5, 17 -- In-Reply-To: {200911141410.nAEEAiQC022121-at-ns.microscopy.com}
5, 17 -- References: {200911141410.nAEEAiQC022121-at-ns.microscopy.com}
5, 17 -- Date: Sun, 22 Nov 2009 11:09:42 -0500
5, 17 -- To: microscopy-at-microscopy.com
5, 17 -- From: ingramp {p.ingram-at-cellbio.duke.edu}
5, 17 -- Subject: Albert Crewe
5, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From qup-at-diver88.freeserve.co.uk Sun Nov 22 15:10:46 2009
Return-Path: {qup-at-diver88.freeserve.co.uk}
Received: from stefan-be6c878b ([89.36.48.155])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAMLAgSZ030024
for {microscopylistserverarchive-at-microscopy.com} ; Sun, 22 Nov 2009 15:10:44 -0600
Received: from [89.36.48.155] by mail-in.freeserve.com; Sun, 22 Nov 2009 13:08:12 -0800

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wadowska-at-upei.ca
Name: Dorota Wadowska

Organization: AVC at the University of PEI

Title-Subject: [Filtered] TME thank you column cleaning

Question: Hello everybody,
I wanted to thank to all of you who responded to my question re:
column cleaning. In my question I was referring to STEM H7000 (sorry
I did not mention that). This scope has a TMP pump which does not
have any oil. This pump crushed and as a result there was quite a few
parts that had to be replaced. The scope is still not operational
(waiting for parts). Recently while changing filament I noticed that
gun housing unit is covered in a fine white powdery substance.
Fluorescent screen was damaged too hence my question about cleaning
the entire column.
Thanks again
Dorota

Login Host: 137.149.102.148
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Nov 23 08:35:40 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nANEZc4s003264
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Nov 2009 08:35:38 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240809c7304e16787f-at-[206.69.208.22]}
6, 11 -- Date: Mon, 23 Nov 2009 08:35:37 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: wadowska-at-upei.ca (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: TME thank you column cleaning
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Mon, 23 Nov 2009 08:37:25 -0600
Subject: [Microscopy] viaWWW: Static Discharge Solution?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David

Organization: SAIC-Frederick

Title-Subject: [Filtered] Discharge Solution?

Question: Hello -

I've recently heard that there's some type of solution that can be
used to disperse static charge on formvar grids?

Has anyone else heard of this, or knows what the solution is? Or am
I off-base, here?

Thanks!

- David

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Nov 23 08:37:25 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nANEbO6b004315
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Nov 2009 08:37:25 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p0624080ac7304e889317-at-[206.69.208.22]}
10, 11 -- Date: Mon, 23 Nov 2009 08:37:24 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Static Discharge Solution?
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: parmiterd-at-mail.nih.gov
Date: Mon, 23 Nov 2009 18:53:51 -0600
Subject: [Microscopy] viaWWW: Liquid Charge Dispersion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David

Organization: SAIC-Frederick

Title-Subject: [Filtered] Liquid Charge Dispersion

Question: Hello again -

Sorry, I guess I wasn't clear regarding my last post:

I'm talking about a liquid that supposedly you can apply to a grid to
reduce the charge.

Anyone heard of this?

Thanks!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Nov 23 18:53:51 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAO0roUD031925
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Nov 2009 18:53:51 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c730df06715b-at-[206.69.208.22]}
10, 11 -- Date: Mon, 23 Nov 2009 18:53:49 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Liquid Charge Dispersion
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ahmad_ds-at-yahoo.com
Date: Tue, 24 Nov 2009 19:53:27 -0600
Subject: [Microscopy] viaWWW: TEM Polymer Sample Preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I assume you mean "antistatic" coating from liquid application - like Duron.


Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
www.ph2llc.com

(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.


-----Original Message-----
X-from: parmiterd-at-mail.nih.gov [mailto:parmiterd-at-mail.nih.gov]
Sent: Monday, November 23, 2009 8:00 PM
To: ph2-at-sprynet.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: parmiterd-at-mail.nih.gov
Name: David

Organization: SAIC-Frederick

Title-Subject: [Filtered] Liquid Charge Dispersion

Question: Hello again -

Sorry, I guess I wasn't clear regarding my last post:

I'm talking about a liquid that supposedly you can apply to a grid to
reduce the charge.

Anyone heard of this?

Thanks!

Login Host: 129.43.43.217
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Nov 23 18:53:51 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nAO0roUD031925
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 23 Nov 2009 18:53:51
-0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240803c730df06715b-at-[206.69.208.22]}
10, 11 -- Date: Mon, 23 Nov 2009 18:53:49 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Liquid Charge Dispersion
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
22, 28 -- From ph2-at-sprynet.com Mon Nov 23 19:06:01 2009
22, 28 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69])
22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAO161wa013807
22, 28 -- for {microscopy-at-microscopy.com} ; Mon, 23 Nov 2009 19:06:01 -0600
22, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
22, 28 -- s=dk20050327; d=sprynet.com;
22, 28 -- b=jXsHRYq6t51aoSOHCDOOX4iUa8KCWYULEPQsmCcs1xxf2rx/D0lD5CX6OMgjfdVx;
22, 28 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP;
22, 28 -- Received: from [99.50.133.46] (helo=user915fa8f284)
22, 28 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67)
22, 28 -- (envelope-from {ph2-at-sprynet.com} )
22, 28 -- id 1NCjqx-0000no-LE; Mon, 23 Nov 2009 20:06:01 -0500
22, 28 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
22, 28 -- To: {parmiterd-at-mail.nih.gov}
22, 28 -- Cc: "Microscopy Listserve" {microscopy-at-microscopy.com}
22, 28 -- Subject: RE: [Microscopy] viaWWW: Liquid Charge Dispersion
22, 28 -- Date: Mon, 23 Nov 2009 20:05:40 -0500
22, 28 -- MIME-Version: 1.0
22, 28 -- Content-Type: text/plain;
22, 28 -- charset="us-ascii"
22, 28 -- Content-Transfer-Encoding: 7bit
22, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
22, 28 -- Thread-Index: AcpsoX3TeL+T2LXHQpKpT4sFs+1BsQAAJiyw
22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
22, 28 -- In-Reply-To: {200911240100.nAO10Jjt006487-at-ns.microscopy.com}
22, 28 -- Message-ID: {E1NCjqx-0000no-LE-at-elasmtp-mealy.atl.sa.earthlink.net}
22, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9aef8e4d29b7f83878349c8334b94fd00350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
22, 28 -- X-Originating-IP: 99.50.133.46
==============================End of - Headers==============================

From quotes.shankarshankar-at-nor-trading.com Tue Nov 24 02:04:04 2009
Return-Path: {quotes.shankarshankar-at-nor-trading.com}
Received: from [202.89.71.71] ([202.89.71.71])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAO83xcL011343;
Tue, 24 Nov 2009 02:04:01 -0600
Received: from [202.89.71.71] by mx2.clients.netdns.net; Tue, 24 Nov 2009 13:31:26 +0530


Colleagues,
dear David, dear Tony, good morning all,

perhaps this might look like arrogant or self-confident but without } googling { I don't know anything about "Duron", as "Tony" meant (see below).

I assume the problem addressed by David is part or at least one piece of mosaic of the } negative stain {-story to reduce hydrophobicity which usually is a property of en-mass pre-fabricated formvar/butvar coated grids.

Most likely he would like to know something about "hydrophilization" of grid surface/"plastic"= Formvar-Carbon film... David: right?

so: IF this is right:

Such hydrophilization can be done either by UV-radiation, which renders carbon coated grids hydrophilic for some hours (up to 4 hrs have been reported), or also "glow discharging" (used routinely in diagnostic EM-Labs doing rapid virus observations)

But: I am aware also of the use of cationic dyes, like e.g. alcian *) blue (AB) from earlier work with the famous retired Dr. Hans Gelderblom RKI Berlin (as well as Paul H...) who always talked about that possibility in case one does not have a "glow discharging unit". ==} Paul Hazelton - as an expert in negative staining procedures - perhaps will/would like to chime in to comment on that technique too.

Also poly-L-lysine seems to be a good alternative (see also below).

Moreover, I would like to paste in here the copied text ( history!!) of

= NetNotes - MICROSCOPY TODAY Vol. 13, No 3, May 2005, p. 64-65 =

---------------------------------------------------------------------------------------------------
} } Formvar Grids (de-charging by glow discharging and other methods) { {

- Q: We have been using Formvar/carbon coated grids for spreading magnetic nano-particles in water and for negative staining. But as you know, an aqueous solution does not spread well on grids because of the charge characteristics on the film surface. The only way I know of for making the film surface more hydrophilic is to do a glow discharge in a sputter coater, but we do not have such a device. I heard treating coated grids with ethanol vapor works, but not for me. Does anyone have any other tricks or suggestions?
Hong Yi {hyi-at-emory.edu} 20 Feb 2005


- Do you have a vacuum evaporator? It's simple to rig it for glow discharge with an inexpensive Tesla coil. A plastic vacuum desiccator, the same Tesla coil, and a rough vacuum source will do the job also.
Caroline Schooley {schooley-at-mcn.org} 21 Feb 2005

(==} The late Charles Garber suggested to use "VICTAWET O")
- I don't think that a splitter coater, old or new, is going to solve your problem. I have not heard of ethanol vapors making a carbon grid more hydrophilic. Carbon coated grids lose their hydrophilic nature as they age and they become more hydrophobic. The process can be "reversed" by
(a) exposure to an RF "air" plasma in a small plasma etcher (effect will last 60-90 days) or
(b) a thin evaporation of } VictawetO { onto the grids. Our own studies would suggest that Victawet can keep the grids highly hydrophilic essentially forever (e.g. more than one year).
- Just remember that it is a phosphate based surfactant so if you are doing elemental analysis work, you might not want to have P showing up in your data. But if you have an ordinary vacuum evaporator and tungsten baskets, and don't have a plasma etcher, you can solve your problem with Victawet. The best bet for having carbon coated grids with the greatest hydrophilic characteristics is to make or purchase your carbon coated grids always "fresh': If the grids are purchased, and their age is uncertain, contact the manufacturer of the carbon coated grids, give them the lot number and then you will know.
Charles A. Garber {cgarber-at-2spi.com} 21 Feb 2005


- Another method occasionally used to make C-coated grids hydrophilic is to expose them for some minutes to the direct illumination of a UV lamp. This is done at normal atmospheric pressure, so it needs no vacuum technology. James Chalcroft {jchalcro-at-neuro.mpg.de} 21 Feb 2005


- I personally don't like glow discharge at all: it's very difficult to reproduce. It depends on the equipment and there is no way to control the "amount" of discharge. I use 0.5-1 % poly-lysine from any of the EM suppliers (don't try to make the solution yourself since there is some trick required). Place the EM grid on a 10 µl poly-lysine drop for 5-10 min, wash on a few drops of deionized water, air dry - good for at least a month. Alcian Blue *) works in the similar way with a similar result. Of course, these methods will only work for positively charged molecules.
Sergey Ryazantsev {sryazant-at-ucla.edu} 22 Feb 2005
------------------------------------------------------------------------
*) for a protocol cf.: xhttp://www.bt.cdc.gov/agent/smallpox/lab-testing/pdf/em-rash-protocol.pdf
(URL out of article "Routine, rapid, noninvasive diagnosis of viral skin exanthems" pp 558-559
==} xhttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2133.2005.07079.x
2005 British Association of Dermatologists * British Journal of Dermatology 2006 154, pp551-577
DOI: 10.1111/j.1365-2133.2005.07079.x


Hoping not to be very wrong or misplaced with my contribution, otherwise perhaps {literature} for specialized purposes.

best wishes and good luck

Wolfgang


=====================================================
Wolfgang MUSS
EM-Lab, Inst. Pathology
SALK-LKH (Gen.Hosp) & PMU(Paracelsus Med.Univ.)
SALZBURG-AUSTRIA

---------------------------------------------------------------------------------------------------------
Ankuendigung namens der - Information on behalf of
SOCIETY for CUTANEOUS ULTRASTRUCTURE RESEARCH (SCUR)
Please visit the updated website of SCUR at } http://www.scur.org {
---------------------------------------------------------------------------------------------------------
Forthcoming in 2010:
+++ 2010, Sep 07th - Sep 08th: 37th Ann. SCUR Meeting HELSINKI +++
First Announcement:
http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/e1029/SCUR2010_Helsinki_meeting_flyer_1_08_2009.pdf

Held before the 40th Annual ESDR meeting - We cordially invite you to participate actively in the meeting.

Go to: Next Meetings at: http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/index_ger.html


=====================================xxxxxxxx==========================================


} -----Ursprüngliche Nachricht-----
} Von: ph2-at-sprynet.com [mailto:ph2-at-sprynet.com]
} Gesendet: Dienstag, 24. November 2009 02:09
} An: Muß Wolfgang
} Betreff: [Microscopy] Re: Static Discharge Solution (on formvar grids)?
} [Again: ] // Liquid Charge Dispersion
} ---------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} I assume you mean "antistatic" coating from liquid application - like Duron
} Tony
} ......................................................................
} Andrew Anthony "Tony" Havics, CHMM, CIH, PE
} pH2, LLC
} 5250 E US 36, Suite 830
} Avon, IN 46123
} www.ph2llc.com
}
} (317) 718-7020 off
} (317) 718-7038 fax
} (317) 409-3238 cell
}
} 90% of Risk Management is knowing where to place the decimal point...anyconsultant can give you the other 10%(SM)


} -----Original Message-----
} X-from: parmiterd-at-mail.nih.gov [mailto:parmiterd-at-mail.nih.gov]
} Sent: Monday, November 23, 2009 8:00 PM
} To: ph2-at-sprynet.com
} Subject: [Microscopy] viaWWW: Liquid Charge Dispersion}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
} Email: parmiterd-at-mail.nih.gov
} Name: David
} Organization: SAIC-Frederick
} Title-Subject: Liquid Charge Dispersion
}
} Question: Hello again -
} Sorry, I guess I wasn't clear regarding my last post:
} I'm talking about a liquid that supposedly you can apply to a grid to
} reduce the charge.

} Anyone heard of this?

} Thanks!





==============================Original Headers==============================
41, 37 -- From W.Muss-at-salk.at Tue Nov 24 03:00:07 2009
41, 37 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9])
41, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAO906JK013391
41, 37 -- for {microscopy-at-microscopy.com} ; Tue, 24 Nov 2009 03:00:06 -0600
41, 37 -- Received: from localhost (localhost [127.0.0.1])
41, 37 -- by hermes.salk.at (Postfix) with ESMTP id B4226C38B6;
41, 37 -- Tue, 24 Nov 2009 10:00:04 +0100 (CET)
41, 37 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at
41, 37 -- Received: from hermes.salk.at ([127.0.0.1])
41, 37 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024)
41, 37 -- with ESMTP id IcyuyIRxd8ie; Tue, 24 Nov 2009 10:00:04 +0100 (CET)
41, 37 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122])
41, 37 -- by hermes.salk.at (Postfix) with ESMTP id 425D1C38B2;
41, 37 -- Tue, 24 Nov 2009 10:00:04 +0100 (CET)
41, 37 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959);
41, 37 -- Tue, 24 Nov 2009 10:00:04 +0100
41, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
41, 37 -- Content-class: urn:content-classes:message
41, 37 -- MIME-Version: 1.0
41, 37 -- Content-Type: text/plain;
41, 37 -- charset="iso-8859-1"
41, 37 -- Subject: [Microscopy] Re: Static Discharge Solution (on formvar grids)? [Again: ] // Liquid Charge Dispersion
41, 37 -- Date: Tue, 24 Nov 2009 10:00:03 +0100
41, 37 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062938AEE-at-N1RZ116.lksdom21.lks.local}
41, 37 -- In-Reply-To: {200911240108.nAO18waw019440-at-ns.microscopy.com}
41, 37 -- X-MS-Has-Attach:
41, 37 -- X-MS-TNEF-Correlator:
41, 37 -- Thread-Topic: [Microscopy] Re: Static Discharge Solution (on formvar grids)? [Again: ] // Liquid Charge Dispersion
41, 37 -- Thread-Index: AcpsoraQC+5LWtVqT/K/e9oAl+hbtQANqF0g
41, 37 -- References: {200911240108.nAO18waw019440-at-ns.microscopy.com}
41, 37 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at}
41, 37 -- To: {ph2-at-sprynet.com}
41, 37 -- Cc: {microscopy-at-microscopy.com}
41, 37 -- X-OriginalArrivalTime: 24 Nov 2009 09:00:04.0338 (UTC) FILETIME=[83391520:01CA6CE4]
41, 37 -- X-Scanned-By: SALK-Content-Filter
41, 37 -- Content-Transfer-Encoding: 8bit
41, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAO906JK013391
==============================End of - Headers==============================

From quotwhats-at-outragegis.com Tue Nov 24 07:52:48 2009
Return-Path: {quotwhats-at-outragegis.com}
Received: from mon24-1-78-224-80-188.fbx.proxad.net (mon24-1-78-224-80-188.fbx.proxad.net [78.224.80.188])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAODqPp1007684;
Tue, 24 Nov 2009 07:52:31 -0600
Received: from [78.224.80.188] by mail.outragegis.com; Tue, 24 Nov 2009 14:49:55 +0100

Hello,

I am using Digital Micrograph (Windows version, perhaps the most or the second recent copy). I would like to know how to export EELS spectra into Photoshop. I want to export line profiles into Photoshop and align/edit several profiles. (I can export profiles with background). Also, I would like to know how to export energy-intensity data set into Excel.

Please advise.

Hiromi
UW-Madison

==============================Original Headers==============================
4, 34 -- From hkonishi-at-wisc.edu Tue Nov 24 10:01:55 2009
4, 34 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210])
4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAOG1tlL013530
4, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Nov 2009 10:01:55 -0600
4, 34 -- MIME-version: 1.0
4, 34 -- Content-transfer-encoding: 7BIT
4, 34 -- Content-disposition: inline
4, 34 -- Content-type: text/plain; CHARSET=US-ASCII
4, 34 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by
4, 34 -- smtpauth1.wiscmail.wisc.edu
4, 34 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009))
4, 34 -- id {0KTM00204F77TN00-at-smtpauth1.wiscmail.wisc.edu} for
4, 34 -- Microscopy-at-microscopy.com; Tue, 24 Nov 2009 10:01:55 -0600 (CST)
4, 34 -- Received: from wiscmail.wisc.edu (wmstore1pvt.pri.doit.wisc.edu [144.92.8.211])
4, 34 -- by smtpauth1.wiscmail.wisc.edu
4, 34 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009))
4, 34 -- with ESMTP id {0KTM0026DF6P2M00-at-smtpauth1.wiscmail.wisc.edu} for
4, 34 -- Microscopy-at-microscopy.com; Tue, 24 Nov 2009 10:01:37 -0600 (CST)
4, 34 -- Received: from [144.92.8.221] (Forwarded-For: 128.104.200.19, [144.92.197.246])
4, 34 -- by wmstore1pvt.pri.doit.wisc.edu (mshttpd); Tue, 24 Nov 2009 10:01:37 -0600
4, 34 -- Date: Tue, 24 Nov 2009 10:01:37 -0600
4, 34 -- From: HIROMI KONISHI {hkonishi-at-wisc.edu}
4, 34 -- Subject: Digital Micrograph
4, 34 -- To: Microscopy-at-microscopy.com
4, 34 -- Message-id: {6f20d4284d617.4b0baf01-at-wiscmail.wisc.edu}
4, 34 -- X-Mailer: Sun Java(tm) System Messenger Express 7u2-7.02 64bit (built Apr 16
4, 34 -- 2009)
4, 34 -- Content-language: en
4, 34 -- X-Accept-Language: en
4, 34 -- Priority: normal
4, 34 -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.211
4, 34 -- X-Spam-PmxInfo: Server=avs-11, Version=5.5.5.374460,
4, 34 -- Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.11.24.155118,
4, 34 -- SenderIP=144.92.8.211
==============================End of - Headers==============================

From quotes-at-linescapes.com Tue Nov 24 13:35:04 2009
Return-Path: {quotes-at-linescapes.com}
Received: from speedtouch.lan ([92.83.44.164])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAOJZ0u1003744
for {microscopylistserverarchive-at-microscopy.com} ; Tue, 24 Nov 2009 13:35:03 -0600
Received: from [92.83.44.164] by mx3.sihope.com; Tue, 24 Nov 2009 21:32:26 +0200
Message-ID: {01ca6d4d$9dc01100$a42c535c-at-quotes}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] Polymer Sample Preparation

Question: Hello Everybody,

I need to examine a polymer composite material, consisting of
high-density polyethylene mixed with nano-scale kaolinite powder,
using the TEM. And I have got to prepare the specimen from a massive
bulk. What is the maximum thickness that would make it transparent to
a 120-kV electron beam? And what is the best preparation method? The
purpose is to check the size and the distribution of these nano
particles within the polymer blulk.


Thank you very much.

Ahmad Ashkaibi

EM Lab. Unit
Materials and Metallurgical Engineering Dept.
Al-Balqa Applied University

Al-Salt - Jordan




Login Host: 79.173.204.196
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Tue Nov 24 19:53:26 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAP1rPgt017282
15, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Nov 2009 19:53:25 -0600
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240800c7323e80d393-at-[206.69.208.22]}
15, 11 -- Date: Tue, 24 Nov 2009 19:53:23 -0600
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: TEM Polymer Sample Preparation
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mtg2002-at-med.cornell.edu
Date: Tue, 24 Nov 2009 19:53:59 -0600
Subject: [Microscopy] viaWWW: Leica EM AC20 Staining Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mtg2002-at-med.cornell.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mtg2002-at-med.cornell.edu
Name: Mike Ganger

Organization: Weill Cornell Medical College

Title-Subject: [Filtered] Leica EM AC20 Staining Question

Question: Greetings everyone,

I am having a problem with fine lead precipitate when using this
stainer. I have changed various options in regards to stain time,
temp, rinse time for both the stain and water, but with limited
success.

I am very diligent in regards to keeping the unit clean and doing
washes pre and post staining, however the precipitate still exists in
varying degrees.

The samples are human tissue using either Epon or Spurrs resin on Cr/Rh grids.

Does anyone who has this unit have any insight as to:

Removing or minimizing the precipitate that does occur.

Is there a recommended set number of uses for both stains as I notice
that contamination becomes more prevalent towards the end of use of
the stain.

Do you or can you use your own stain over the supplied stain from Leica?

Any help would be most appreciated.

Thanks in advance,

Mike Ganger
Weill Cornell Medical College



Login Host: 140.251.32.156
---------------------------------------------------------------------------

==============================Original Headers==============================
18, 11 -- From zaluzec-at-microscopy.com Tue Nov 24 19:53:59 2009
18, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
18, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAP1rwZB017684
18, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Nov 2009 19:53:59 -0600
18, 11 -- Mime-Version: 1.0
18, 11 -- Message-Id: {p06240801c7323e9eda95-at-[206.69.208.22]}
18, 11 -- Date: Tue, 24 Nov 2009 19:53:58 -0600
18, 11 -- To: microscopy-at-microscopy.com
18, 11 -- From: mtg2002-at-med.cornell.edu (by way of MicroscopyListserver)
18, 11 -- Subject: viaWWW: Leica EM AC20 Staining Question
18, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 25 Nov 2009 12:08:47 -0600
Subject: [Microscopy] Need fluorescent stain for lignin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ahmad,

Best would be if you had access to a cryo-ultramicrotome. A cut of 50-100nm
placed on a support grid should do the job.

Best regards,

Petra
__________________________________
Dr. Petra Wahlbring
Lead Engineer Material Development
GIC*L
Colmar-Berg, Luxembourg
e-mail: petra.wahlbring-at-goodyear.com
tel: +352 8199 3725
fax: +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be
copied or disseminated without the express written consent of The Goodyear
Tire & Rubber Company. -



ahmad_ds-at-yahoo.co
m
To
11/25/09 02:58 AM petra.wahlbring-at-goodyear.com
cc

Please respond to Subject
ahmad_ds-at-yahoo.co [Microscopy] viaWWW: TEM Polymer
m Sample Preparation













----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: ahmad_ds-at-yahoo.com
Name: Ahmad Ashkaibi

Organization: Al-Balqa Applied University

Title-Subject: [Filtered] Polymer Sample Preparation

Question: Hello Everybody,

I need to examine a polymer composite material, consisting of
high-density polyethylene mixed with nano-scale kaolinite powder,
using the TEM. And I have got to prepare the specimen from a massive
bulk. What is the maximum thickness that would make it transparent to
a 120-kV electron beam? And what is the best preparation method? The
purpose is to check the size and the distribution of these nano
particles within the polymer blulk.


Thank you very much.

Ahmad Ashkaibi

EM Lab. Unit
Materials and Metallurgical Engineering Dept.
Al-Balqa Applied University

Al-Salt - Jordan




Login Host: 79.173.204.196
---------------------------------------------------------------------------

==============================Original
Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Tue Nov 24 19:53:26 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id nAP1rPgt017282
15, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Nov 2009
19:53:25 -0600
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240800c7323e80d393-at-[206.69.208.22]}
15, 11 -- Date: Tue, 24 Nov 2009 19:53:23 -0600
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: ahmad_ds-at-yahoo.com (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: TEM Polymer Sample Preparation
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================



==============================Original Headers==============================
33, 16 -- From petra.wahlbring-at-goodyear.com Wed Nov 25 02:48:20 2009
33, 16 -- Received: from emeam1.goodyear.com (emeam1.goodyear.com [167.232.242.40])
33, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAP8mKvt029771
33, 16 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Nov 2009 02:48:20 -0600
33, 16 -- In-Reply-To: {200911250158.nAP1wNAJ027719-at-ns.microscopy.com}
33, 16 -- Subject: Re: [Microscopy] viaWWW: TEM Polymer Sample Preparation
33, 16 -- To: ahmad_ds-at-yahoo.com
33, 16 -- Cc: Microscopy-at-Microscopy.Com
33, 16 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005
33, 16 -- Message-ID: {OFE045D771.E9E8C53E-ONC1257679.002F85EC-C1257679.00305BD2-at-goodyear.com}
33, 16 -- From: petra.wahlbring-at-goodyear.com
33, 16 -- Date: Wed, 25 Nov 2009 09:48:11 +0100
33, 16 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 8.0.1 HF1200|May 11, 2009) at
33, 16 -- 11/25/2009 09:48:23 AM
33, 16 -- MIME-Version: 1.0
33, 16 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================

From quoctuant-at-alco.ca Wed Nov 25 06:04:26 2009
Return-Path: {quoctuant-at-alco.ca}
Received: from 93-38-151-184.ip71.fastwebnet.it (93-38-151-184.ip71.fastwebnet.it [93.38.151.184])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAPC4MKl019527
for {microscopylistserverarchive-at-microscopy.com} ; Wed, 25 Nov 2009 06:04:25 -0600
Received: from [93.38.151.184] by mail.alco.ca; Wed, 25 Nov 2009 13:01:47 +0100

I apologize for cross-posting...

A student here wants to find a fluorescent dye for lignin so that she can
see it on her (mostly red) autofluorescing grass. The grass will be
subjected to various harsh treatments to rid it of the lignin, but so far
it seems that all the treatments result in lignin being redopisited on the
material in droplets. We can see the droplets with SEM, but would like to
find a good way to quantify the amount of remaining cells walls
(cellulose) that is covered by lignin droplets. I think the ultimate goal
is to use image analysis to get some numbers.

Any ideas?

Aloha from sunny and warm Honolulu,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
6, 20 -- From tina-at-pbrc.hawaii.edu Wed Nov 25 12:08:47 2009
6, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7])
6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAPI8kgM002763
6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Nov 2009 12:08:47 -0600
6, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1])
6, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id nAPI7wNs027549
6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
6, 20 -- Wed, 25 Nov 2009 08:07:58 -1000 (HST)
6, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id nAPI7vck027546;
6, 20 -- Wed, 25 Nov 2009 08:07:57 -1000 (HST)
6, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs
6, 20 -- Date: Wed, 25 Nov 2009 08:07:56 -1000 (HST)
6, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
6, 20 -- X-Sender: tina-at-halia
6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
6, 20 -- CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU
6, 20 -- Subject: Need fluorescent stain for lignin
6, 20 -- Message-ID: {Pine.GSO.4.21.0911250802300.27515-100000-at-halia}
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 25 Nov 2009 13:03:34 -0600
Subject: [Microscopy] Re: Need fluorescent stain for lignin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tina,

Calcofluor White 2MR stains cell walls & fluoresces white, but I'm
not sure if it is specific to lignin.
Acidic phloroglucinol is a standard lignin stain, and it looks like
it should be fluorescent, but I'm not sure (3 hydroxy groups bound to
alternate carbons of a benzene ring).
http://www.botany.hawaii.edu/faculty/Webb/BOT410/410Labs/LabsHTML-99/Xylem/Labxyphlo99.html
Should be easy enough to get some and find out.

But, have you checked for autofluorescence? That's a common problem
with plant cell walls, and if I remember right, lignin is a
contributor to this.

Phil

} I apologize for cross-posting...
}
} A student here wants to find a fluorescent dye for lignin so that she can
} see it on her (mostly red) autofluorescing grass. The grass will be
} subjected to various harsh treatments to rid it of the lignin, but so far
} it seems that all the treatments result in lignin being redopisited on the
} material in droplets. We can see the droplets with SEM, but would like to
} find a good way to quantify the amount of remaining cells walls
} (cellulose) that is covered by lignin droplets. I think the ultimate goal
} is to use image analysis to get some numbers.
}
} Any ideas?
}
} Aloha from sunny and warm Honolulu,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
6, 25 -- From oshel1pe-at-cmich.edu Wed Nov 25 13:03:34 2009
6, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAPJ3Yb5018884
6, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Nov 2009 13:03:34 -0600
6, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nAPJ3WSw007113
6, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Nov 2009 14:03:33 -0500
6, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Wed, 25 Nov 2009 14:03:31 -0500
6, 25 -- Mime-Version: 1.0
6, 25 -- Message-Id: {f06240805c7332e5cc112-at-[141.209.160.249]}
6, 25 -- In-Reply-To: {Pine.GSO.4.21.0911250802300.27515-100000-at-halia}
6, 25 -- References: {Pine.GSO.4.21.0911250802300.27515-100000-at-halia}
6, 25 -- Date: Wed, 25 Nov 2009 14:03:28 -0500
6, 25 -- To: Microscopy-at-microscopy.com
6, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 25 -- Subject: Re: Need fluorescent stain for lignin
6, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 25 -- X-OriginalArrivalTime: 25 Nov 2009 19:03:32.0413 (UTC) FILETIME=[FB549ED0:01CA6E01]
6, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 25 -- X-Spam-Score: -4.40 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
6, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 25 -- X-CanItPRO-Stream: default
6, 25 -- X-Canit-Stats-ID: 23177609 - 2cd8c5f4b0dd - 20091125
6, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Fri, 27 Nov 2009 07:32:10 -0600
Subject: [Microscopy] Re: viaWWW: SEM prep of bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both caitlin.otto-at-asu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: caitlin.otto-at-asu.edu
Name: Caitlin Otto

Organization: Arizona State University

Title-Subject: [Filtered] SEM prep of bacteria

Question: We're having some difficulties with a standard SEM prep of
bacterial cells. We have tried fixing the cells in suspension with
gluteraldehyde followed by application to poly-l-lysine cover slips;
and we have tried applying live cells to cover slips followed by
fixation. When cells are fixed in suspension they have very low
contrast and surface features are absent. When cells are fixed on
cover slips there's a uniform bumpy texture. We are uncertain about
whether this texture is due to the fixation process or is native to
the cells. Has anyone had any experience with this fixation
procedure. Any suggestions are greatly appreciated.

Thank you!


Login Host: 129.219.191.194
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Nov 25 16:36:45 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAPMajAi012625
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Nov 2009 16:36:45 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240808c73361d21c24-at-[206.69.208.22]}
8, 11 -- Date: Wed, 25 Nov 2009 16:36:44 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: caitlin.otto-at-asu.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: SEM prep of bacteria
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From quoteform-at-rackspace.co.uk Thu Nov 26 00:59:32 2009
Return-Path: {quoteform-at-rackspace.co.uk}
Received: from nexbase (121.246.26.254.dynamic.chennai.vsnl.net.in [121.246.26.254] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQ6xTX4027951;
Thu, 26 Nov 2009 00:59:30 -0600
Received: from [121.246.26.254] by mx2.dfw1.rackspace.com; Thu, 26 Nov 2009 12:26:51 +0530

Hi there!
I'm new to the list (and the topic!),
so apologize in advance if I'm asking something well-known
and already stored somewhere.

I would appreciate some help
(direct suggestions, redirection to web resources etc.)
on how to prepare samples of bacterial biofilm
grown in the oral cavity ontop of dental restoration material,
for both Atomic Force Microscopy
and Two-Photon Confocal Fluorescence Optical Microscopy.

For example, for AFM, is it better to fixate the bacterial cells
with gluteraldeyde or similar, or just image them in physiological
solution?
If one wants to appreciate contrast with methods different from simple
topographic profiling
(e.g. image the stiffness, adhesion, friction, phase, or possibly other
"chemical" signals),
I imagine that the closer to the live conditions the cells are, the
better
(i.e. least denaturation with least loss of possible contrast factors).

Also, as I'm completely new to fluorescence:
what the best markers for this kinf of cells (mouth and saliva bacteria)
are,
and what the most appropriate moment and method to stain them?
Can them still be stained after fixation?

Sorry for the maybe stupid questions!
(I'm an AFMer of inorganics and polymers,
never worked with cells before!)

Thank you so much in advance for any help.
Regards,
Marco.


xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr. Marco Salerno,
SPM Lab responsible,
Nanophys Unit -
Nanobio Department,
Italian Institute of Technology
via Morego 30, Bolzaneto
16163 Genova - ITALY
marco.salerno-at-iit.it
iitspmlab.bravehost.com
Lab.+39-010-71.781.756
Off.+39-010-71.781.444
Fax +39-010-72.03.21
Mob.+39-349-26.75.277
Home+39-010-97.61.344
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



==============================Original Headers==============================
10, 23 -- From marco.salerno-at-iit.it Thu Nov 26 01:51:40 2009
10, 23 -- Received: from cfexch.netexchange.int.netscalibur.it (cfexch.netexchange.int.netscalibur.it [62.196.3.49])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQ7pdFm031081
10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 26 Nov 2009 01:51:39 -0600
10, 23 -- Received: from EDEN-CLU.netexchange.int.netscalibur.it ([192.168.228.55]) by cfexch.netexchange.int.netscalibur.it with Microsoft SMTPSVC(6.0.3790.3959);
10, 23 -- Thu, 26 Nov 2009 08:51:38 +0100
10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 23 -- Content-class: urn:content-classes:message
10, 23 -- MIME-Version: 1.0
10, 23 -- Content-Type: text/plain;
10, 23 -- charset="us-ascii"
10, 23 -- Subject: Help with sample prep protocol of bacterial biofilm for AFM/fluorescence
10, 23 -- Date: Thu, 26 Nov 2009 08:51:37 +0100
10, 23 -- Message-ID: {5B2D6D6DDD42414A91B85B082D7C5FC40127B705-at-EDEN-CLU.netexchange.int.netscalibur.it}
10, 23 -- X-MS-Has-Attach:
10, 23 -- X-MS-TNEF-Correlator:
10, 23 -- Thread-Topic: Help with sample prep protocol of bacterial biofilm for AFM/fluorescence
10, 23 -- Thread-Index: AcpuaLVRFW9lQenGT6Oed2DZ2lGwSgABH56Q
10, 23 -- From: "Salerno Marco" {marco.salerno-at-iit.it}
10, 23 -- To: {microscopy-at-microscopy.com}
10, 23 -- X-OriginalArrivalTime: 26 Nov 2009 07:51:38.0385 (UTC) FILETIME=[48B5E010:01CA6E6D]
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAQ7pdFm031081
==============================End of - Headers==============================

From quotas-at-alcoa.com.au Thu Nov 26 03:26:38 2009
Return-Path: {quotas-at-alcoa.com.au}
Received: from ABTS-North-Dynamic-217.249.163.122.airtelbroadband.in (ABTS-North-Dynamic-217.249.163.122.airtelbroadband.in [122.163.249.217] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQ9QYAo017455;
Thu, 26 Nov 2009 03:26:37 -0600
Received: from [122.163.249.217] by na-msw3.alcoa.com; Thu, 26 Nov 2009 01:23:59 -0800

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mallikarjun.karadge-at-ge.com as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mallikarjun.karadge-at-ge.com
Name: Mallikarjun Karadge

Organization: GE Global Research

Title-Subject: [Filtered] Research Scientist -
Materials Characterization Position Available at
GE Global Research India

Question: Research Scientist (Materials Characterization)


General Electric - Global Research (Bangalore,
India) has an immediate opportunity for a
Research Scientist in the area of Advanced
Materials Characterization.

The following would be a typical profile of the successful candidate:

Should have a PhDÝinÝMetallurgy /
MaterialsÝScience or a Masters with minimum 4
years of relevant experience.

Demonstrates hands-on expertise in Transmission
Electron Microscopy (Analytical and HRTEM),
Scanning Electron Microscopy (including Electron
Back Scattered Diffraction) and X-Ray diffraction
techniques. Experience in AFM, surface
characterization and compositional analysis would
be an added advantage.

Has an in-depth
knowledgeÝofÝelectronÝdiffractionÝtheory,
crystallography, quantitative stereology,
defectÝimaging (dislocations, SFs etc). Domain
knowledge in one or more of the following areas
is essential: structural alloys, coatings,
tribology, ceramics / electronic materials.

Since the position demands versatility with
materials, adaptability to a variety of
engineering problems and multitasking
capabilities, the candidate should be able to
demonstrate sufficient expertise / experience
across multiple domains and a passion for problem
solving.

Cover letter and resume can be submitted online
at http://www.ge.com/careers/ (Job Number:
1093762) Mallikarjun.Karadge-at-ge.com (Dr.
Mallikarjun Karadge) Gopi.Chandran-at-ge.com (Dr.
Gopi Chandran)

About GE Global Research:

GE Global Research has been the cornerstone of GE
technology for more than 100 years. We are one of
the worldís largest and most diverse industrial
research labs with a presence that spans the
globe.

We have close to 2,800 of the best and brightest
researchers spread out at four multi-disciplinary
facilities around the world. Headquartered in
Niskayuna, New York, we also have facilities in
Bangalore, India; Shanghai, China; and Munich,
Germany. Weíre delivering the innovations and
breakthroughs that are driving growth for GEís
businesses and revolutionizing markets. We
believe ìwhat we imagine, we can make happen.î

We are an Equal Opportunity Employer.

_________________________________________________

Mallikarjun Karadge, Ph.D.

Lead Materials Scientist,
General Electric Global Research.

John. F. Welch Technology Center,
#122, Export Promotion Industrial Park, Phase 2,
Hoodi Village, Whitefield Road, Bangalore-560066,
India

T +91-80-4012-2691
F +91-80-2841-1111
E: mallikarjun.karadge-at-ge.com
www.ge.com/research



Login Host: 220.227.64.166
---------------------------------------------------------------------------


==============================Original Headers==============================
26, 14 -- From zaluzec-at-microscopy.com Thu Nov 26 09:11:24 2009
26, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
26, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQFBLAT031672
26, 14 -- for {microscopy-at-microscopy.com} ; Thu, 26 Nov 2009 09:11:24 -0600
26, 14 -- Mime-Version: 1.0
26, 14 -- Message-Id: {p06240801c7344ae4bd3c-at-[206.69.208.22]}
26, 14 -- Date: Thu, 26 Nov 2009 09:11:20 -0600
26, 14 -- To: microscopy-at-microscopy.com
26, 14 -- From: mallikarjun.karadge-at-ge.com (by way of MicroscopyListserver)
26, 14 -- Subject: viaWWW: Research Scientist - Materials Characterization Position
26, 14 -- Available at GE Global Research India
26, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
26, 14 -- Content-Transfer-Encoding: 8bit
26, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAQFBLAT031672
==============================End of - Headers==============================

From quotes-at-digital-page.com Thu Nov 26 15:21:29 2009
Return-Path: {quotes-at-digital-page.com}
Received: from complex (dlj93.neoplus.adsl.tpnet.pl [83.24.39.93])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQLLOXo024620
for {microscopylistserverarchive-at-microscopy.com} ; Thu, 26 Nov 2009 15:21:27 -0600
Received: from [83.24.39.93] by spam.digital-page.com; Thu, 26 Nov 2009 22:18:46 +0100

How are you drying the cells? That could be the source of the texture
difference. Or have you tried cryo-methods? Is the fixation
identical, other than the cells being in suspension or attached to
coverslips? Are they fixed from the same solution? Meaning: are the
suspended cells fixed in media, and the attached cells fixed in
buffer? You could be fixing serum or other components of the medium
to the suspended cells -- but the stuff is usually fibrous.
The texture difference could also be from retained extracellular
components in the suspension-fixed cells that are lost on the
attached-fixed cells. Non-obvious why, but I've seen similar things,
and your reported absence of surface features makes me thing they're
covered by something.
This is assuming your SEM operating parameters are the same. What are
they? That will determine what you see.

I've always found texture on bacterial cells, regardless of how I've
dried or fixed them, so I suspect it's real -- but can be made more
obvious ("worse") by improper fixation or drying, or under the beam.
Be sure to use the lowest kV and probe current you can.

Phil

} Email: caitlin.otto-at-asu.edu
} Name: Caitlin Otto
}
} Organization: Arizona State University
}
} Title-Subject: [Filtered] SEM prep of bacteria
}
} Question: We're having some difficulties with a standard SEM prep of
} bacterial cells. We have tried fixing the cells in suspension with
} gluteraldehyde followed by application to poly-l-lysine cover slips;
} and we have tried applying live cells to cover slips followed by
} fixation. When cells are fixed in suspension they have very low
} contrast and surface features are absent. When cells are fixed on
} cover slips there's a uniform bumpy texture. We are uncertain about
} whether this texture is due to the fixation process or is native to
} the cells. Has anyone had any experience with this fixation
} procedure. Any suggestions are greatly appreciated.
}
} Thank you!
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 26 -- From oshel1pe-at-cmich.edu Fri Nov 27 07:32:09 2009
4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nARDW96o023127
4, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Nov 2009 07:32:09 -0600
4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nARDW4Lw026700;
4, 26 -- Fri, 27 Nov 2009 08:32:07 -0500
4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 26 -- Fri, 27 Nov 2009 08:31:28 -0500
4, 26 -- Mime-Version: 1.0
4, 26 -- Message-Id: {f06240800c735830a6786-at-[141.209.160.249]}
4, 26 -- In-Reply-To: {200911252240.nAPMeSdU018899-at-ns.microscopy.com}
4, 26 -- References: {200911252240.nAPMeSdU018899-at-ns.microscopy.com}
4, 26 -- Date: Fri, 27 Nov 2009 08:31:24 -0500
4, 26 -- To: caitlin.otto-at-asu.edu
4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 26 -- Subject: Re: [Microscopy] viaWWW: SEM prep of bacteria
4, 26 -- Cc: Microscopy-at-microscopy.com
4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 26 -- X-OriginalArrivalTime: 27 Nov 2009 13:31:28.0921 (UTC) FILETIME=[ECD48090:01CA6F65]
4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 26 -- X-Spam-Score: -4.40 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
4, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 26 -- X-CanItPRO-Stream: default
4, 26 -- X-Canit-Stats-ID: 23235725 - e87da0b800dd - 20091127
4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Mon, 30 Nov 2009 14:46:06 -0600
Subject: [Microscopy] Need fluorescent stain for lignin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think that fixing bacteria in suspension can create artefact.
Therefore, we use polycarbonate filter coated with poly-l-lysine to
capture non-pathogenic bacteria which are fixed with glutaraldehyde,
dehydrated in ethanol, CPD and gold coated. However, I would fix do
pathogens in a suspension. The question is: suspension in what medium?
Would that medium interact with glutaraldehyde? To find out, what is the
problem, I would also use another fixative such as formaldehyde or
OSO4-Ruthenium Red to fix the capsule.

I will send you an attachment of Conventional Scanning Electron
Microscopy of Bacteria, containing the detail method, published in
infocus magazine (the proceedings of the Royal Microscopical Society)
Issue10 June 2008 P44-61. Those who want a copy may write to me
off-line.

Ann Fook Yang
-----Original Message-----
X-from: caitlin.otto-at-asu.edu [mailto:caitlin.otto-at-asu.edu]
Sent: Wednesday, November 25, 2009 5:45 PM
To: Yang, Ann-Fook

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both caitlin.otto-at-asu.edu as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: caitlin.otto-at-asu.edu
Name: Caitlin Otto

Organization: Arizona State University

Title-Subject: [Filtered] SEM prep of bacteria

Question: We're having some difficulties with a standard SEM prep of
bacterial cells. We have tried fixing the cells in suspension with
gluteraldehyde followed by application to poly-l-lysine cover slips;
and we have tried applying live cells to cover slips followed by
fixation. When cells are fixed in suspension they have very low
contrast and surface features are absent. When cells are fixed on
cover slips there's a uniform bumpy texture. We are uncertain about
whether this texture is due to the fixation process or is native to
the cells. Has anyone had any experience with this fixation
procedure. Any suggestions are greatly appreciated.

Thank you!


Login Host: 129.219.191.194
------------------------------------------------------------------------
---

==============================Original
Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Wed Nov 25 16:36:45 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id nAPMajAi012625
8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Nov 2009
16:36:45 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240808c73361d21c24-at-[206.69.208.22]}
8, 11 -- Date: Wed, 25 Nov 2009 16:36:44 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: caitlin.otto-at-asu.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: SEM prep of bacteria
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of -
Headers==============================


==============================Original Headers==============================
15, 23 -- From Ann-Fook.Yang-at-AGR.GC.CA Fri Nov 27 09:41:13 2009
15, 23 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118])
15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nARFfDwt010675
15, 23 -- for {microscopy-at-microscopy.com} ; Fri, 27 Nov 2009 09:41:13 -0600
15, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
15, 23 -- Content-class: urn:content-classes:message
15, 23 -- MIME-Version: 1.0
15, 23 -- Content-Type: text/plain;
15, 23 -- charset="us-ascii"
15, 23 -- Subject: RE: [Microscopy] viaWWW: SEM prep of bacteria
15, 23 -- Date: Fri, 27 Nov 2009 10:41:12 -0500
15, 23 -- Message-ID: {773CE84BBA86484A9FEDE7741B77DDA401617572-at-onottaxms1.AGR.GC.CA}
15, 23 -- In-Reply-To: {200911252245.nAPMj9ds026605-at-ns.microscopy.com}
15, 23 -- X-MS-Has-Attach:
15, 23 -- X-MS-TNEF-Correlator:
15, 23 -- Thread-Topic: [Microscopy] viaWWW: SEM prep of bacteria
15, 23 -- Thread-Index: AcpuIPGdBtk/mJogQcqnCDvdeguwUABT5gwQ
15, 23 -- From: "Yang, Ann-Fook" {Ann-Fook.Yang-at-AGR.GC.CA}
15, 23 -- To: {microscopy-at-microscopy.com}
15, 23 -- X-OriginalArrivalTime: 27 Nov 2009 15:41:13.0251 (UTC) FILETIME=[0CA6FF30:01CA6F78]
15, 23 -- Received-SPF: none
15, 23 -- Content-Transfer-Encoding: 8bit
15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nARFfDwt010675
==============================End of - Headers==============================

From quocpham-at-ortondirect.com Fri Nov 27 12:44:38 2009
Return-Path: {quocpham-at-ortondirect.com}
Received: from [190.223.169.238] ([190.223.169.238])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nARIiTHs031646
for {microscopylistserverarchive-at-microscopy.com} ; Fri, 27 Nov 2009 12:44:35 -0600
Received: from [190.223.169.238] by server532.appriver.com; Fri, 27 Nov 2009 13:41:52 -0500

I agree with Phil. Lignin autofluoresces. Calcofluor is typically used
for cellulose identification (under UV light) and Phloroglucinol-HCL
for lignin (at the light level, no fluorescence needed). Here is Zheng-
Hua Ye's protocol for lignin staining:
Add a drop of 1% phloroglucinol in 100% ethanol onto a section and
then add a drop of conc. HCl. Lignin will be stained red in a few
minutes.

And, check out Ruiqin Zhong's paper on Ectopic Deposition of Lignin in
the Pith of Stems of Two Arabidopsis Mutants. http://www.plantphysiol.org/cgi/content/full/123/1/59
or you email me for the pdf.

best,
Beth

On Nov 25, 2009, at 2:03 PM, oshel1pe-at-cmich.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Tina,
}
} Calcofluor White 2MR stains cell walls & fluoresces white, but I'm
} not sure if it is specific to lignin.
} Acidic phloroglucinol is a standard lignin stain, and it looks like
} it should be fluorescent, but I'm not sure (3 hydroxy groups bound to
} alternate carbons of a benzene ring).
} http://www.botany.hawaii.edu/faculty/Webb/BOT410/410Labs/LabsHTML-99/Xylem/Labxyphlo99.html
} Should be easy enough to get some and find out.
}
} But, have you checked for autofluorescence? That's a common problem
} with plant cell walls, and if I remember right, lignin is a
} contributor to this.
}
} Phil
}
} } I apologize for cross-posting...
} }
} } A student here wants to find a fluorescent dye for lignin so that
} } she can
} } see it on her (mostly red) autofluorescing grass. The grass will be
} } subjected to various harsh treatments to rid it of the lignin, but
} } so far
} } it seems that all the treatments result in lignin being redopisited
} } on the
} } material in droplets. We can see the droplets with SEM, but would
} } like to
} } find a good way to quantify the amount of remaining cells walls
} } (cellulose) that is covered by lignin droplets. I think the
} } ultimate goal
} } is to use image analysis to get some numbers.
} }
} } Any ideas?
} }
} } Aloha from sunny and warm Honolulu,
} } Tina
} }
} } ****************************************************************************
} } * Tina (Weatherby) Carvalho *
} } tina-at-pbrc.hawaii.edu *
} } * Biological Electron Microscope Facility * (808)
} } 956-6251 *
} } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} } ****************************************************************************
}
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576



**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
Miller Plant Sciences Bldg
120 Carlton Street
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com






==============================Original Headers==============================
18, 21 -- From beth-at-plantbio.uga.edu Mon Nov 30 14:46:05 2009
18, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAUKk5ra004353
18, 21 -- for {microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 14:46:05 -0600
18, 21 -- Received: from [128.192.26.46] ([128.192.26.46])
18, 21 -- (authenticated user beth-at-plantbio.uga.edu)
18, 21 -- by dogwood.plantbio.uga.edu
18, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
18, 21 -- for microscopy-at-microscopy.com;
18, 21 -- Mon, 30 Nov 2009 15:45:59 -0500
18, 21 -- Message-Id: {39493DA5-C683-4E25-8E5F-8FE15E282185-at-plantbio.uga.edu}
18, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
18, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com}
18, 21 -- In-Reply-To: {200911251903.nAPJ3w98019716-at-ns.microscopy.com}
18, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
18, 21 -- Content-Transfer-Encoding: 7bit
18, 21 -- Mime-Version: 1.0 (Apple Message framework v936)
18, 21 -- Subject: Re: [Microscopy] Re: Need fluorescent stain for lignin
18, 21 -- Date: Mon, 30 Nov 2009 15:46:03 -0500
18, 21 -- References: {200911251903.nAPJ3w98019716-at-ns.microscopy.com}
18, 21 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: bigelow-at-umich.edu
Date: Mon, 30 Nov 2009 15:50:05 -0600
Subject: [Microscopy] RE: Faraday cup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A few days ago someone inquired about a Faraday cup for a CM20 TEM.
Several years ago I designed a Faraday cup for the JEOL 2010 TEM that
allowed the beam current to be recorded with an external picoammeter,
independently of the circuitry of the microscope. If all else fails,
it might be possible to adapt this design to the CM20.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

==============================Original Headers==============================
1, 15 -- From bigelow-at-umich.edu Mon Nov 30 15:50:05 2009
1, 15 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82])
1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAULo5E6021707
1, 15 -- for {microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 15:50:05 -0600
1, 15 -- Received: FROM [99.26.106.105] (adsl-99-26-106-105.dsl.sfldmi.sbcglobal.net [99.26.106.105])
1, 15 -- By hellskitchen.mr.itd.umich.edu ID 4B143E0C.7CE1A.8241 ;
1, 15 -- Authuser bigelow;
1, 15 -- 30 Nov 2009 16:50:04 EST
1, 15 -- Mime-Version: 1.0
1, 15 -- Message-Id: {p06240801c739ed6760bc-at-[99.26.106.105]}
1, 15 -- Date: Mon, 30 Nov 2009 16:49:33 -0500
1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 15 -- Subject: [Microscopy] RE: Faraday cup
1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bobwcarter-at-gmail.com
Date: Mon, 30 Nov 2009 18:00:20 -0600
Subject: [Microscopy] Old chemicals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is Spring Cleaning time here. Yes, I know spring has sprung, but
being an incurable procrastinator, I figure I am right on time.

We have been blessed with an abundance of chemicals, both donated and
purchased by us. Some are getting long in the tooth, and I am
struggling with the question of culling the herd.

Specifically, I am guessing that reagent powders in their original
containers are good forever, or at least until they look really bad.

How about fixatives? We have old bottles of 50% glut. Some in the
refer, some frozen, do you think they are still good? What about any
other fixatives?

Same for resin components. We have some old half empty bottles from
way back, some old unopened bottles, and some 'current' bottles. Any
guidelines on how to tell if they are OK. Do they last forever? How
about combining half full bottles? I know I can always mix up a batch
to see if it comes out OK, but if there is any advice out there, I
would like to hear from you.

I know there will be many opinions on this topic. One of them will be
that I should just test everything, but I am hoping there might be
some suggestions that will make my task a little easier.

Thanks

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
12, 37 -- From jkrupp-at-deltacollege.edu Mon Nov 30 16:06:43 2009
12, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150])
12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nAUM6g3L004404
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 16:06:43 -0600
12, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1])
12, 37 -- by localhost (Email Security Appliance) with SMTP id 8349117569B_B1441F2B
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 22:06:42 +0000 (GMT)
12, 37 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236])
12, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 74B19170831_B1441F2F
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 22:06:40 +0000 (GMT)
12, 37 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
12, 37 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
12, 37 -- with ESMTP id 50710801 for Microscopy-at-microscopy.com; Mon, 30 Nov 2009 14:06:31 -0800
12, 37 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by
12, 37 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with
12, 37 -- ESMTP id KTY02U00.PU9 for {Microscopy-at-microscopy.com} ; Mon, 30
12, 37 -- Nov 2009 14:06:30 -0800
12, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id F04D57A38464
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 14:06:30 -0800 (PST)
12, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu
12, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
12, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024)
12, 37 -- with ESMTP id xu+lJNQOqEuF for {Microscopy-at-microscopy.com} ;
12, 37 -- Mon, 30 Nov 2009 14:06:30 -0800 (PST)
12, 37 -- Received: from [172.20.3.214] (unknown [172.20.3.214])
12, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C4B6F7A383BB
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 14:06:30 -0800 (PST)
12, 37 -- Message-Id: {ECAF1858-482A-4F63-A5BB-2E7DB8E0E598-at-deltacollege.edu}
12, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
12, 37 -- To: Microscopy-at-microscopy.com
12, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
12, 37 -- Content-Transfer-Encoding: 7bit
12, 37 -- Mime-Version: 1.0 (Apple Message framework v936)
12, 37 -- Subject: Old chemicals
12, 37 -- Date: Mon, 30 Nov 2009 14:06:30 -0800
12, 37 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================

From quoc-at-artesmagicae.com Mon Nov 30 17:01:42 2009
Return-Path: {quoc-at-artesmagicae.com}
Received: from pamo-2bde5ac418 (adsl190-2643072.dyn.etb.net.co [190.26.43.72] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAUN1c9R021848;
Mon, 30 Nov 2009 17:01:40 -0600
Received: from [190.26.43.72] by artesmagicae.com; Mon, 30 Nov 2009 23:58:52 +0100

Dear Jon,

Some ether compounds become unstable with age. They could spontaniously
burn or even explode. Ether compounds have the structure R-O-R where R is
carbon chain or group and O is oxygen. Care should be used in handling or
disposal of older ethers.
.
Bob Carter
Bellingham, WA 98226


----- Original Message -----
X-from: jkrupp-at-deltacollege.edu
To: bobwcarter-at-gmail.com
Sent: Monday, November 30, 2009 2:12 PM

It is Spring Cleaning time here. Yes, I know spring has sprung, but
being an incurable procrastinator, I figure I am right on time.

We have been blessed with an abundance of chemicals, both donated and
purchased by us. Some are getting long in the tooth, and I am
struggling with the question of culling the herd.

Specifically, I am guessing that reagent powders in their original
containers are good forever, or at least until they look really bad.

How about fixatives? We have old bottles of 50% glut. Some in the
refer, some frozen, do you think they are still good? What about any
other fixatives?

Same for resin components. We have some old half empty bottles from
way back, some old unopened bottles, and some 'current' bottles. Any
guidelines on how to tell if they are OK. Do they last forever? How
about combining half full bottles? I know I can always mix up a batch
to see if it comes out OK, but if there is any advice out there, I
would like to hear from you.

I know there will be many opinions on this topic. One of them will be
that I should just test everything, but I am hoping there might be
some suggestions that will make my task a little easier.

Thanks

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp-at-deltacollege.edu




==============================Original Headers==============================
12, 37 -- From jkrupp-at-deltacollege.edu Mon Nov 30 16:06:43 2009
12, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu
[207.62.178.150])
12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
nAUM6g3L004404
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 16:06:43 -0600
12, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain
[127.0.0.1])
12, 37 -- by localhost (Email Security Appliance) with SMTP id
8349117569B_B1441F2B
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 22:06:42 +0000
(GMT)
12, 37 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us
[207.62.178.236])
12, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id
74B19170831_B1441F2F
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 22:06:40 +0000
(GMT)
12, 37 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us)
12, 37 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9)
12, 37 -- with ESMTP id 50710801 for Microscopy-at-microscopy.com; Mon, 30
Nov 2009 14:06:31 -0800
12, 37 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by
12, 37 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15)
with
12, 37 -- ESMTP id KTY02U00.PU9 for {Microscopy-at-microscopy.com} ;
Mon, 30
12, 37 -- Nov 2009 14:06:30 -0800
12, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id F04D57A38464
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 14:06:30 -0800
(PST)
12, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu
12, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1])
12, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new,
port 10024)
12, 37 -- with ESMTP id xu+lJNQOqEuF for {Microscopy-at-microscopy.com} ;
12, 37 -- Mon, 30 Nov 2009 14:06:30 -0800 (PST)
12, 37 -- Received: from [172.20.3.214] (unknown [172.20.3.214])
12, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C4B6F7A383BB
12, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 14:06:30 -0800
(PST)
12, 37 -- Message-Id:
{ECAF1858-482A-4F63-A5BB-2E7DB8E0E598-at-deltacollege.edu}
12, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu}
12, 37 -- To: Microscopy-at-microscopy.com
12, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed;
delsp=yes
12, 37 -- Content-Transfer-Encoding: 7bit
12, 37 -- Mime-Version: 1.0 (Apple Message framework v936)
12, 37 -- Subject: Old chemicals
12, 37 -- Date: Mon, 30 Nov 2009 14:06:30 -0800
12, 37 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================


==============================Original Headers==============================
23, 47 -- From bobwcarter-at-gmail.com Mon Nov 30 18:00:20 2009
23, 47 -- Received: from mail-px0-f188.google.com (mail-px0-f188.google.com [209.85.216.188])
23, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB100JWW024349
23, 47 -- for {microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 18:00:19 -0600
23, 47 -- Received: by pxi26 with SMTP id 26so3304619pxi.21
23, 47 -- for {microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 16:00:19 -0800 (PST)
23, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
23, 47 -- d=gmail.com; s=gamma;
23, 47 -- h=domainkey-signature:received:received:message-id:from:to:cc
23, 47 -- :references:subject:date:mime-version:content-type
23, 47 -- :content-transfer-encoding:x-priority:x-msmail-priority:x-mailer
23, 47 -- :x-mimeole;
23, 47 -- bh=poai10X3QjnfNC7ux4M83YVmunk77tvVreVrAnAy3Nw=;
23, 47 -- b=U1qh50F2sJF47KtrByLgtVe1yQSCHZ86syYDx8GS48Dci02/03mXeF16WsBB8voA2d
23, 47 -- q935prH/bo0k8TYJ36eUVJQDKfxakcJzZHC/EaVm/oZdm3HD8Q9UnjFR8FNGdXNTM3HJ
23, 47 -- TlwJ2eWeCOQB/XA3MIeunxPsTqDUFC9OJTJMI=
23, 47 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
23, 47 -- d=gmail.com; s=gamma;
23, 47 -- h=message-id:from:to:cc:references:subject:date:mime-version
23, 47 -- :content-type:content-transfer-encoding:x-priority:x-msmail-priority
23, 47 -- :x-mailer:x-mimeole;
23, 47 -- b=byzG5JJAO0IZYnArg9ghySxtDzkmD0QNtOMT89idNrxNfBkTF72zrtx+nWtjzZegEj
23, 47 -- bqoubItxu+BIPFTdnRt0v7FvVLJx8Z18AOVoU40E5cOgg4XLWCt35ZSXDzCU95+rzQHn
23, 47 -- lMJiPAspfT+NQkew3JpBd6gnztV4AwAVCJp9U=
23, 47 -- Received: by 10.115.133.38 with SMTP id k38mr9122598wan.120.1259625618992;
23, 47 -- Mon, 30 Nov 2009 16:00:18 -0800 (PST)
23, 47 -- Received: from bigkitty (174-21-65-3.tukw.qwest.net [174.21.65.3])
23, 47 -- by mx.google.com with ESMTPS id 23sm141724pxi.9.2009.11.30.16.00.16
23, 47 -- (version=TLSv1/SSLv3 cipher=RC4-MD5);
23, 47 -- Mon, 30 Nov 2009 16:00:17 -0800 (PST)
23, 47 -- Message-ID: {EFFF47843E4D44B18028F86D6D301601-at-bigkitty}
23, 47 -- From: "Bob Carter" {bobwcarter-at-gmail.com}
23, 47 -- To: {jkrupp-at-deltacollege.edu}
23, 47 -- Cc: "Microscopy" {microscopy-at-microscopy.com}
23, 47 -- References: {200911302212.nAUMCmeC015313-at-ns.microscopy.com}
23, 47 -- Subject: Re: [Microscopy] Old chemicals
23, 47 -- Date: Mon, 30 Nov 2009 15:59:55 -0800
23, 47 -- MIME-Version: 1.0
23, 47 -- Content-Type: text/plain;
23, 47 -- format=flowed;
23, 47 -- charset="iso-8859-1";
23, 47 -- reply-type=original
23, 47 -- Content-Transfer-Encoding: 7bit
23, 47 -- X-Priority: 3
23, 47 -- X-MSMail-Priority: Normal
23, 47 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843
23, 47 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
==============================End of - Headers==============================




From: garyeaston-at-scannerscorp.com
Date: Wed, 2 Dec 2009 06:48:30 -0600
Subject: [Microscopy] Polaron E5000 Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mark.talbot-at-csiro.au as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mark.talbot-at-csiro.au
Name: Mark Talbot

Organization: CSIRO Plant Industry

Title-Subject: [Filtered] Beam stability

Question: Hi, I'd like to get an idea of people's experience with EDS
X-ray analysis with Zeiss EVO SEMs, in terms of beam stability.
Contact me off-list if you like. Many thanks,
Mark

Login Host: 152.83.194.1
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Mon Nov 30 19:03:12 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB113Bo4007834
6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Nov 2009 19:03:11 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240804c73a1bb6b562-at-[206.69.208.22]}
6, 11 -- Date: Mon, 30 Nov 2009 19:03:11 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: mark.talbot-at-csiro.au (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Beam stability & EDS
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 0-49257062.0025fae3-at-suzuyo.co.jp Tue Dec 1 00:09:46 2009
Return-Path: {0-49257062.0025fae3-at-suzuyo.co.jp}
Received: from abs-static-234.120.68.58.aircel.co.in (abs-static-234.120.68.58.aircel.co.in [58.68.120.234] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB169ga5031730;
Tue, 1 Dec 2009 00:09:45 -0600
Received: from [104.82.158.10] (helo=iswnndnkn.rgdxvgfrtzh.info)
by abs-static-234.120.68.58.aircel.co.in with esmtpa (Exim 4.69)
(envelope-from )
id 1MMSD9-5898ld-8A
for microscopylistserverarchive-at-microscopy.com; Tue, 1 Dec 2009 11:39:45 +0530
Message-ID: {0044265019.8BVGB8KB014864-at-lnyylpwptg.iulsiiyjgedk.net}

Cell Biology Education is a refereed online journal published by the
American Society for Cell Biology. The current issue has an
interesting article, which includes instructions for making the
microscope:

Using a Replica of Leeuwenhoek's Microscope to Teach the History of
Science and to Motivate Students to Discover the Vision and the
Contributions of the First Microscopists
Lenira M.N. Sepel, Elgion L.S. Loreto, and Joao B.T. Rocha
CBE Life Sci Educ 2009;8 338-343
http://www.lifescied.org/cgi/content/abstract/8/4/338?etoc
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
45301 Caspar Point Road, Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
2, 15 -- From schooley-at-mcn.org Tue Dec 1 16:46:13 2009
2, 15 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85])
2, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB1MkCRL030868
2, 15 -- for {Microscopy-at-Microscopy.Com} ; Tue, 1 Dec 2009 16:46:13 -0600
2, 15 -- Received: from [66.81.40.100]
2, 15 -- by smtp1.mcn.org with esmtpa (Exim 4.63)
2, 15 -- (envelope-from {schooley-at-mcn.org} )
2, 15 -- id 1NFbU3-0000Fd-5N; Tue, 01 Dec 2009 14:46:12 -0800
2, 15 -- Mime-Version: 1.0
2, 15 -- Message-Id: {a06200707c73b4ba27590-at-[66.81.40.100]}
2, 15 -- Date: Tue, 1 Dec 2009 14:46:33 -0800
2, 15 -- To: Microscopy-at-Microscopy.Com
2, 15 -- From: Caroline Schooley {schooley-at-mcn.org}
2, 15 -- Subject: Microscopy Education
2, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From quotes-at-cartersurveying.com Tue Dec 1 17:46:14 2009
Return-Path: {quotes-at-cartersurveying.com}
Received: from casa (c9502616.virtua.com.br [201.80.38.22] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB1NkBBU014114;
Tue, 1 Dec 2009 17:46:13 -0600
Received: from [201.80.38.22] by cartersurveying.com; Tue, 1 Dec 2009 20:43:23 -0300

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both chaueter-at-bcm.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: chaueter-at-bcm.edu
Name: Claire Haueter

Organization: HHMI-Baylor College of Medicine

Title-Subject: [Filtered] Cryosectioning of Drosophila Tissues

Question: Hello,

Our cryosectioning attachment has arrived and I will get hands on
training sometime late next week.
I mainly work with Drosophila tissues and I would like to get your
advice on the most suitable tissue for the training. Eventually, we
want to do Tokuyasu sectioning/immunolabelling method on thawed thin
cryosections from third instar wing discs, adult brain, adult eye,
and embryo. The samples will be chemically fixed then cryoprotected
with sucrose prior to freezing followed by sectioning.

For those who have handled Drosophila tissues for cryosectioning:
Which tissue is suitable and easier for the training?
Would you share with me cryosectioning tips for drosophila tissues?
Is it ok to leave chemically fixed samples in sucrose solution until
ready for cryosectioning?

Thank you in advance,

Claire

Login Host: 128.249.107.38
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Tue Dec 1 20:08:35 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB228XER017367
10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 1 Dec 2009 20:08:34 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240802c73b7c916a16-at-[206.69.208.22]}
10, 11 -- Date: Tue, 1 Dec 2009 20:08:31 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: chaueter-at-bcm.edu (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Cryosectioning of Drosophila Tissues
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From quotes-at-creins.ca Wed Dec 2 04:15:49 2009
Return-Path: {quotes-at-creins.ca}
Received: from PC-200901301416 ([60.218.197.70])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB2AFiM9017317;
Wed, 2 Dec 2009 04:15:46 -0600
Received: from [60.218.197.70] by m2.spamarrest.com; Wed, 2 Dec 2009 18:12:56 +0800

Hi Listers,
I'm looking for the operator manual for the E5000 coater. If anyone
has a copy on PDF or Word, I would appreciate a copy. Thanks in advance.

Gary Easton


==============================Original Headers==============================
3, 19 -- From garyeaston-at-scannerscorp.com Wed Dec 2 06:48:30 2009
3, 19 -- Received: from omr7.networksolutionsemail.com (omr7.networksolutionsemail.com [205.178.146.57])
3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB2CmU7f021615
3, 19 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2009 06:48:30 -0600
3, 19 -- Received: from mail.networksolutionsemail.com (mail.networksolutionsemail.com [205.178.146.50])
3, 19 -- by omr7.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id nB2CmTAH003432
3, 19 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2009 07:48:29 -0500
3, 19 -- Received: (qmail 13364 invoked by uid 78); 2 Dec 2009 12:48:29 -0000
3, 19 -- Received: from unknown (HELO ?192.168.1.3?) (72.81.190.77)
3, 19 -- by ns-omr1.lb.hosting.dc2.netsol.com with SMTP; 2 Dec 2009 12:48:29 -0000
3, 19 -- Message-ID: {4B16620F.4030509-at-scannerscorp.com}
3, 19 -- Date: Wed, 02 Dec 2009 07:48:15 -0500
3, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
3, 19 -- MIME-Version: 1.0
3, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 19 -- Subject: Polaron E5000 Sputter Coater
3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: eschumacher-at-mccrone.com
Date: Thu, 3 Dec 2009 08:19:20 -0600
Subject: [Microscopy] Short Course Announcement: Advanced SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ciao Mario!

If there was a precise answer for your questions our work would be so much easier!
I think that you should try both fixed and unfixed material, and then you can work out an explanation of what you saw/measured.
If you fix your material you can reasonably expect to modify some surface parameters.

As for staining, it all depends on what you want to label. If you want to label the whole bacteria in order to see them, you may try a general DNA dye like DAPI or Hoechst. They may fade pretty fast in confocal microscopy though, so be careful.

Tanti auguri di buon natale

Stephane



----- Original Message ----
X-from: "marco.salerno-at-iit.it" {marco.salerno-at-iit.it}
To: nizets2-at-yahoo.com
Sent: Thu, November 26, 2009 8:56:53 AM

Hi there!
I'm new to the list (and the topic!),
so apologize in advance if I'm asking something well-known
and already stored somewhere.

I would appreciate some help
(direct suggestions, redirection to web resources etc.)
on how to prepare samples of bacterial biofilm
grown in the oral cavity ontop of dental restoration material,
for both Atomic Force Microscopy
and Two-Photon Confocal Fluorescence Optical Microscopy.

For example, for AFM, is it better to fixate the bacterial cells
with gluteraldeyde or similar, or just image them in physiological
solution?
If one wants to appreciate contrast with methods different from simple
topographic profiling
(e.g. image the stiffness, adhesion, friction, phase, or possibly other
"chemical" signals),
I imagine that the closer to the live conditions the cells are, the
better
(i.e. least denaturation with least loss of possible contrast factors).

Also, as I'm completely new to fluorescence:
what the best markers for this kinf of cells (mouth and saliva bacteria)
are,
and what the most appropriate moment and method to stain them?
Can them still be stained after fixation?

Sorry for the maybe stupid questions!
(I'm an AFMer of inorganics and polymers,
never worked with cells before!)

Thank you so much in advance for any help.
Regards,
Marco.


xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr. Marco Salerno,
SPM Lab responsible,
Nanophys Unit -
Nanobio Department,
Italian Institute of Technology
via Morego 30, Bolzaneto
16163 Genova - ITALY
marco.salerno-at-iit.it
iitspmlab.bravehost.com
Lab.+39-010-71.781.756
Off.+39-010-71.781.444
Fax +39-010-72.03.21
Mob.+39-349-26.75.277
Home+39-010-97.61.344
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



==============================Original Headers==============================
10, 23 -- From marco.salerno-at-iit.it Thu Nov 26 01:51:40 2009
10, 23 -- Received: from cfexch.netexchange.int.netscalibur.it (cfexch.netexchange.int.netscalibur.it [62.196.3.49])
10, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nAQ7pdFm031081
10, 23 --     for {microscopy-at-microscopy.com} ; Thu, 26 Nov 2009 01:51:39 -0600
10, 23 -- Received: from EDEN-CLU.netexchange.int.netscalibur.it ([192.168.228.55]) by cfexch.netexchange.int.netscalibur.it with Microsoft SMTPSVC(6.0.3790.3959);
10, 23 --     Thu, 26 Nov 2009 08:51:38 +0100
10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 23 -- Content-class: urn:content-classes:message
10, 23 -- MIME-Version: 1.0
10, 23 -- Content-Type: text/plain;
10, 23 --     charset="us-ascii"
10, 23 -- Subject: Help with sample prep protocol of bacterial biofilm for AFM/fluorescence
10, 23 -- Date: Thu, 26 Nov 2009 08:51:37 +0100
10, 23 -- Message-ID: {5B2D6D6DDD42414A91B85B082D7C5FC40127B705-at-EDEN-CLU.netexchange.int.netscalibur.it}
10, 23 -- X-MS-Has-Attach:
10, 23 -- X-MS-TNEF-Correlator:
10, 23 -- Thread-Topic: Help with sample prep protocol of bacterial biofilm for AFM/fluorescence
10, 23 -- Thread-Index: AcpuaLVRFW9lQenGT6Oed2DZ2lGwSgABH56Q
10, 23 -- From: "Salerno Marco" {marco.salerno-at-iit.it}
10, 23 -- To: {microscopy-at-microscopy.com}
10, 23 -- X-OriginalArrivalTime: 26 Nov 2009 07:51:38.0385 (UTC) FILETIME=[48B5E010:01CA6E6D]
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nAQ7pdFm031081
==============================End of - Headers==============================






==============================Original Headers==============================
27, 25 -- From nizets2-at-yahoo.com Wed Dec 2 07:04:16 2009
27, 25 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
27, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nB2D4FsK004030
27, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2009 07:04:15 -0600
27, 25 -- Received: (qmail 43431 invoked by uid 60001); 2 Dec 2009 13:04:14 -0000
27, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1259759054; bh=MS6SUWS8XY6QOpNOF1k64jchxBihda9aw+p5CJ552no=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=2Pmfbe7xfDI5XP77Hzj2X8ZBDKh9lTGOh+bjPJKFS5jOqNbtgz+9YOzHvRAF9PoJ+ndg8DJeyP/wIqpBEzcwI07zV6R7hkXsCAwx67efl6DRvokSJrEj2HolPLgcZgscIyTMat1ruedZJdHO2ablUgfHLU9slMwKWkDcn03q728=
27, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
27, 25 -- s=s1024; d=yahoo.com;
27, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
27, 25 -- b=eHUtwjxNJLwA+akBLXYNAFO0VRSeJx9F3I618SugGqcTf3dxSe/8CgGX1hrEpUtmtyxfDP7j9DjrFEq8W0CxR5z+9929kZa86cSaYHF7KhDoUAbJvBGveBP3SPxB9HfBvKEgnsVpV4uriiB42zU1uuhYc97LwjJENIXs0j/TtkA=;
27, 25 -- Message-ID: {472100.42857.qm-at-web110814.mail.gq1.yahoo.com}
27, 25 -- X-YMail-OSG: eRIGwu4VM1nNLFihYUZe4mVBJlxsWzgkL0V94ldBWCDaoe6lyXxl6RJ0ybo1epvGvwNv2syrs6HqCJbhZW8Qwf7anAbsoVH1xd3Ydi_LDaf3VUFnWd0Nd9ncOP52vfuvnr3l.hZzHFbA2Fp0tdotiXa3.iyvLpz1uBMvac21ckxm1Qe0bmP7UO6c.JlG7BBAdWQDGaKcYceMaJqlvqfKH8ciDkPG4rG0XWdCZjWSRuwFR99wpdWeg95AnFqQYaKc_euvhrcA5VgFoX8OJLfyy7eJcHyfmMlaenCkY6r0Q6Hds0mEHv5cHXuojPGhNgByU4JXZJ1Y3S3BDrLtcVcz219u6cjr4qIMpU2_SgHbbwsYVZJxKxB9gjq_4VUvfajrOkoeEnDTlaYeIHFo1MDj2UXUm6G3AC8WKjfZS.4nvRfG_fjSyPj0aHjnvi.9PZaWL6ptsNI6zSiOyrFqN1EcNQ7r.1FEoRr2RhE056flzRZ0eBdNWfducFUnJruo2ywXKWaDm1MDTf27x8sr4h7UeDj9ix.0IKNbdqLmt0gAJb6RWDeAYdJX43F02ygdqFDqSk3RrQEMjo6f8SLpDZVjBzlvUVNkkr1iFQMRjFa5t5G.JIJy3BMkEw3lq8mOLlwCjEu7L.JrENTclZI-
27, 25 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Wed, 02 Dec 2009 05:04:14 PST
27, 25 -- X-Mailer: YahooMailRC/211.6 YahooMailWebService/0.8.100.260964
27, 25 -- References: {200911260756.nAQ7urM6005039-at-ns.microscopy.com}
27, 25 -- Date: Wed, 2 Dec 2009 05:04:14 -0800 (PST)
27, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
27, 25 -- Subject: Re: [Microscopy] Help with sample prep protocol of bacterial biofilm for AFM/fluorescence
27, 25 -- To: marco.salerno-at-iit.it
27, 25 -- Cc: microscopy-at-microscopy.com
27, 25 -- In-Reply-To: {200911260756.nAQ7urM6005039-at-ns.microscopy.com}
27, 25 -- MIME-Version: 1.0
27, 25 -- Content-Type: text/plain; charset=iso-8859-1
27, 25 -- Content-Transfer-Encoding: 8bit
27, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB2D4FsK004030
==============================End of - Headers==============================

From 0-syolanda.hernandezalonso-at-telefonica.es Wed Dec 2 08:24:50 2009
Return-Path: {0-syolanda.hernandezalonso-at-telefonica.es}
Received: from viettel.vn (adsl.viettel.vn [115.74.189.183] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB2EOjLg021059;
Wed, 2 Dec 2009 08:24:48 -0600
Received: from [118.183.103.155] (account 0-0-0-0-contact-at-marie-reberat.com HELO zohqgfykjxgngf.aoupc.tv)
by viettel.vn (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 133862995 for microscopylistserverarchive-at-microscopy.com; Wed, 2 Dec 2009 21:24:47 +0700

Hi All

I am in Africa where the email is not so good so I have just noted the
conversation on the Faraday cup.

You may remember my comments in the past on the use of the photometer
characteristics of the TEM screen (monitored in Pa)? Whilst this does not
give the beam current at the specimen it could be used as a guide to enable
repetitive experimentation at a constant current.

Every TEM has one and its free!

Steve Chapman FRMS
Senior Consultant
Protrain for Electron Microscopy Consultancy and Training world wide
Tel +44 1280816512 Fax +44 1280814007
Cell +44 7711606967 Web www.emcourses.com

----- Original Message -----
X-from: {bigelow-at-umich.edu}
To: {protrain-at-emcourses.com}
Sent: Monday, November 30, 2009 9:50 PM

Greetings Colleagues,

COM210: ADVANCED SCANNING ELECTRON MICROSCOPE BASED TOOLS FOR MICROANALYSIS

The College of Microscopy is offering an Advanced SEM course the week of March 15th 2010. This course concentrates on X-ray spectrometry, both energy dispersive and wavelength dispersive, especially as they pertain to chemical or spectral mapping. It also covers electron backscattered diffraction analysis, and touch on methods such as Monte Carlo modeling, cathodoluminescence, and sample preparation and transfer.

Those familiar with SEM imaging, but wanting to expand their expertise with these analytical methods benefit from this course. Students considering adding these techniques to their laboratory also gain valuable background knowledge. Students learn through lectures, demonstrations by instructors and their own, hands-on instrument operation. Students are encouraged to bring samples with them to the course so they can learn using their own real world samples.



For more information regarding this course please visit the College of Microscopy's website or click on the link below.



http://www.collegeofmicroscopy.com/courses/course.asp?COURSE_ID=111




*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com}
Web Site: www.mccrone.com {http://www.mccrone.com/}



*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************



==============================Original Headers==============================
15, 23 -- From eschumacher-at-mccrone.com Thu Dec 3 08:19:20 2009
15, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114])
15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB3EJK3q002514
15, 23 -- for {microscopy-at-microscopy.com} ; Thu, 3 Dec 2009 08:19:20 -0600
15, 23 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by
15, 23 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Thu, 3 Dec 2009 08:19:20
15, 23 -- -0600
15, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com}
15, 23 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
15, 23 -- Date: Thu, 3 Dec 2009 08:19:17 -0600
15, 23 -- Subject: Short Course Announcement: Advanced SEM
15, 23 -- Thread-Topic: Short Course Announcement: Advanced SEM
15, 23 -- Thread-Index: Acp0I5kMqq9b+pvESn+3dOGlfQTdxA==
15, 23 -- Message-ID: {874B1DB532886444A60A015EE363493F0C78FCDA74-at-mccronemsg07.tmg.mccrone.com}
15, 23 -- Accept-Language: en-US
15, 23 -- Content-Language: en-US
15, 23 -- X-MS-Has-Attach:
15, 23 -- X-MS-TNEF-Correlator:
15, 23 -- acceptlanguage: en-US
15, 23 -- Content-Type: text/plain; charset="us-ascii"
15, 23 -- MIME-Version: 1.0
15, 23 -- Content-Transfer-Encoding: 8bit
15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB3EJK3q002514
==============================End of - Headers==============================




From: pal.baggethun-at-elkem.no
Date: Mon, 7 Dec 2009 19:15:49 -0600
Subject: [Microscopy] ViaWWW: LM: particles in optically dense medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,
Thanks everyone for the great response to my request.

Gary Easton

==============================Original Headers==============================
2, 19 -- From garyeaston-at-scannerscorp.com Thu Dec 3 09:33:07 2009
2, 19 -- Received: from omr10.networksolutionsemail.com (omr10.networksolutionsemail.com [205.178.146.60])
2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB3FX7e4018738
2, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Dec 2009 09:33:07 -0600
2, 19 -- Received: from mail.networksolutionsemail.com (mail.networksolutionsemail.com [205.178.146.50])
2, 19 -- by omr10.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id nB3FX6jc017953
2, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Dec 2009 10:33:07 -0500
2, 19 -- Received: (qmail 23404 invoked by uid 78); 3 Dec 2009 15:33:06 -0000
2, 19 -- Received: from unknown (HELO ?192.168.1.3?) (72.81.190.77)
2, 19 -- by ns-omr1.lb.hosting.dc2.netsol.com with SMTP; 3 Dec 2009 15:33:06 -0000
2, 19 -- Message-ID: {4B17DA22.3090104-at-scannerscorp.com}
2, 19 -- Date: Thu, 03 Dec 2009 10:32:50 -0500
2, 19 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
2, 19 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
2, 19 -- MIME-Version: 1.0
2, 19 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
2, 19 -- Subject: Polaron E5000 Sputter Coater Manual
2, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
2, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From 0-2ortilla-at-disrayco.com Thu Dec 3 09:42:32 2009
Return-Path: {0-2ortilla-at-disrayco.com}
Received: from home ([132.248.99.211])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB3FgS3e001028;
Thu, 3 Dec 2009 09:42:31 -0600
Received: from [205.114.144.182] (account 0-ac0000-at-umail.hinet.net HELO lrpwe.mcewcke.ua)
by home (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 468012904 for microscopylistserverarchive-at-microscopy.com; Thu, 3 Dec 2009 09:42:31 -0600
Message-ID: {3121549259.UC69ANHY947199-at-lhlhfqwh.phnhlsm.ua}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mjamison-at-caissonlabs.com {mjamison-at-caissonlabs.com
as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mjamison-at-caissonlabs.com {mjamison-at-caissonlabs.com
Name: Michelle Jamison

Title-Subject: [Filtered] used LCM purchase questions

Question: Hi everyone,

The lab I work at is interested in purchasing a LCM. We are
considering a used model with the following components:
Arcturus Pixcell II laser capture
Microdissection system including:
PXL 200 pixel II instrument, Serial #0409
Olympus lens 1: UPLANFI 4x0.13,
Lens 2: :UPLANFI 10x/0.30
Lens 3: LCPLANFI 20x0.40;
Lens 4: LCPLANFI 40x/0.60
PXL-200 Controller, Serial #0409
Hitachi Digital comera, Model #KP-D580
Olympus Replacement Burner, Model #u-uls100hg, Serial #9f19980
Olympus High Pressure Mercury Burner, Model #BH2-RFL-T3 Serail# 903084
Dell PC, Sony Trinitron Monitor, Power Supply

I hear this could be a good deal at $28,000 but have very limited
exposure to this type of thing. Could someone tell me what I should
do to ensure this is a good machine. Our concern is that planed
obsolescence may soon make the instrument expensive to maintain.
Which components, and what aspects, should I be looking at to make
this assessment?
Thank you for any direction you can give.



Michelle


Login Host: 129.123.46.133
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 12 -- From zaluzec-at-microscopy.com Fri Dec 4 18:00:10 2009
11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB5009Ke008496
11, 12 -- for {microscopy-at-microscopy.com} ; Fri, 4 Dec 2009 18:00:10 -0600
11, 12 -- Mime-Version: 1.0
11, 12 -- Message-Id: {p06240800c73f52f6ae48-at-[206.69.208.22]}
11, 12 -- Date: Fri, 4 Dec 2009 18:00:08 -0600
11, 12 -- To: microscopy-at-microscopy.com
11, 12 -- From: "mjamison-at-caissonlabs.com" {mjamison-at-caissonlabs.com ()} (by way of
11, 12 -- MicroscopyListserver)
11, 12 -- Subject: viaWWW: used LCM purchase questions
11, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 0-ka-at-ps.ge.com Fri Dec 4 21:19:13 2009
Return-Path: {0-ka-at-ps.ge.com}
Received: from desktop (Dynamic-IP-cr200118163209.cable.net.co [200.118.163.209] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB53J8Ao027008;
Fri, 4 Dec 2009 21:19:12 -0600
Received: from [139.169.40.97] (account 0-fileone-at-somewhere.com HELO dffzbkhmjj.wtbdqtmyswjachj.org)
by desktop (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 701176400 for microscopylistserverarchive-at-microscopy.com; Fri, 4 Dec 2009 22:19:12 -0500
Message-ID: {4725640698.7EIC3IM6847805-at-wuiqrowclcgsby.gcogjuzjzjjblda.org}

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Thikra_bio-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Thikra_bio-at-yahoo.com
Name: Thikra Mustafa

Organization: phD student -at- UALR

Title-Subject: [Filtered] Questions

Question:
I have samples of DNA/CNT specimen suspended in TE Buffer. we faced
problem when we are trying prepared the samples for SEM & AFM. the TE
buffer not drying properly. could you please answer how we can get
rid of this problem?

we can replace this type of buffer with another one?


Login Host: 144.167.108.84
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Sat Dec 5 10:30:01 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB5GTxIF016605
8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 5 Dec 2009 10:30:01 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240804c7403ae80c0f-at-[206.69.208.22]}
8, 11 -- Date: Sat, 5 Dec 2009 10:29:58 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: Thikra_bio-at-yahoo.com (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: specimen suspended in TE Buffer
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 0-d_to_find_tools-at-mail.vresp.com Sat Dec 5 18:45:11 2009
Return-Path: {0-d_to_find_tools-at-mail.vresp.com}
Received: from pc01 (201-254-113-59.speedy.com.ar [201.254.113.59] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB60iw5r010404;
Sat, 5 Dec 2009 18:45:09 -0600
Received: from 201.254.113.59 by mailin2.allianz.es; Sat, 5 Dec 2009 21:45:09 -0300

Hi All-
I'm posting this for a colleague at the main Cornell campus....

Is there anyone out there, in the general vicinity of Ithaca,NY (or
at least closer than NYC is to Ithaca), who has an HPF and who
accepts clients from other institutions? Better still...do you have
any experience with plant material? This last would be the icing on
the cake, but not a deal-killer.

If you can help, please contact Maria Harrison at mjh78-at-cornell.edu.

As always,
thanks!
Lee
--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
5, 31 -- From lcgould-at-med.cornell.edu Mon Dec 7 15:25:41 2009
5, 31 -- Received: from mail-gw1.med.cornell.edu (mail-gw1.med.cornell.edu [140.251.3.44])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB7LPfjv014775
5, 31 -- for {microscopy-at-microscopy.com} ; Mon, 7 Dec 2009 15:25:41 -0600
5, 31 -- MIME-version: 1.0
5, 31 -- Content-transfer-encoding: 7BIT
5, 31 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed
5, 31 -- Received: from mpx5.med.cornell.edu ([140.251.11.120])
5, 31 -- by mail-gw1.med.cornell.edu
5, 31 -- (Sun Java(tm) System Messaging Server 6.3-8.03 (built Apr 24 2009; 32bit))
5, 31 -- with ESMTP id {0KUA00K7RWUN6330-at-mail-gw1.med.cornell.edu} for
5, 31 -- microscopy-at-microscopy.com; Mon, 07 Dec 2009 16:25:39 -0500 (EST)
5, 31 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
5, 31 -- by mpx5.med.cornell.edu
5, 31 -- (Sun Java(tm) System Messaging Server 7u2-7.04 64bit (built Jul 2 2009))
5, 31 -- with ESMTPA id {0KUA00GQFWUN9130-at-mpx5.med.cornell.edu} for
5, 31 -- microscopy-at-microscopy.com; Mon, 07 Dec 2009 16:25:37 -0500 (EST)
5, 31 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379,
5, 31 -- Antispam-Data: 2009.12.7.211818
5, 31 -- X-Perlmx-Spam: Gauge=, IIIIIIII, Probability=8%,
5, 31 -- Report=' BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0,
5, 31 -- BODY_SIZE_7000_LESS 0, BODY_SIZE_800_899 0, __C230066_P5 0,
5, 31 -- __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MEDS_PLAIN 0,
5, 31 -- __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0,
5, 31 -- __SANE_MSGID 0, __TO_MALFORMED_2 0, __URI_NS '
5, 31 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
5, 31 -- Message-id: {p06230914c743226c23f4-at-[140.251.48.23]}
5, 31 -- Date: Mon, 07 Dec 2009 16:25:31 -0500
5, 31 -- To: Microscopy Listserver {microscopy-at-microscopy.com}
5, 31 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
5, 31 -- Subject: HPF in the Finger Lakes region
==============================End of - Headers==============================

From t23-at-doae.go.th Mon Dec 7 16:27:16 2009
Return-Path: {t23-at-doae.go.th}
Received: from cpc2-mfld9-0-0-cust494.nott.cable.ntl.com (cpc2-mfld9-0-0-cust494.nott.cable.ntl.com [82.21.193.239])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB7MQJl8031270;
Mon, 7 Dec 2009 16:26:55 -0600
Received: from 82.21.193.239 by mail.doae.go.th; Mon, 7 Dec 2009 22:27:15 +0000

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pal.baggethun-at-elkem.no as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pal.baggethun-at-elkem.no
Name: P. Baggethun

Organization: Elkem Solar Research

Title-Subject: [Filtered] LM: particles in optically dense medium

Question: Suppose you have opaque particles distributed throughout a
transparent medium (considerably more optically dense than air) and
want to measure the particle size distribution by light microscopy by
acquiring and analyzing images at a number of stage positions
(z-positions).

Does anyone have ready at hand the relationship between the
refractive index of the medium and the magnification factor of any
particle as a function of depth z and position xy in the stage plane
and the wavelength of the light, assuming that the optical properties
of the objective lens is known?

Login Host: 212.4.33.171
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Dec 7 19:15:49 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB81Fltx008500
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 7 Dec 2009 19:15:48 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240801c74359222532-at-[206.69.208.22]}
7, 11 -- Date: Mon, 7 Dec 2009 19:15:46 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: pal.baggethun-at-elkem.no (by way of MicroscopyListserver)
7, 11 -- Subject: ViaWWW: LM: particles in optically dense medium
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rschmitz-at-uwsp.edu
Date: Tue, 8 Dec 2009 18:10:06 -0600
Subject: [Microscopy] viaWWW: LIECA UC6 knife approach problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lgsimmerman-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lgsimmerman-at-gmail.com
Name: Lauren Simmerman

Organization: EM Labs, Inc.

Title-Subject: [Filtered] TEM-Help with Fading Slides

Question: Hi,
It has been several years since I have posted a question, but since
the member's answers helped me last time, I am hoping for the same
result this time!

We are a diagnostic pathology, private EM lab. A quick run down of
our processing is: washing the sample in Sorenson's Phosphated
Buffer, osmium tetroxide, water wash, dehydration series in acetone,
Spurr Low Viscocity resin. The blocks cure overnight in a 65-70
degree C oven.

Our semi-thin sections are stained with Basic Fuchsin/Methylene
Blue(~ 10 sec. with each stain on a 250 degree F hotplate). The
staining pattern is similar to an H&E. The results are beautiful.
Glycogen, collogen, & nuclei are blue with nucleoli, cytoplasm,
mitochondria, & basal lamina bright pink.

The problem became apparent about a year ago when we noticed that
after cover-slipping with Cytoseal-60, a low viscocity mounting
medium, the blue and pink faded, leaving the sections with a
homogenous purple color.

What would be the cause of the sections turning colors once
cover-slipped? If left un-covered the color stays perfect.
We have thought about going back to Toluidine Blue, but the
pathologists prefer the results of the BFMB stain when it works
properly.

Thanks for any help with this.

Lauren Simmerman
EM Labs, Inc.
Birmingham, AL
205-939-9741





Login Host: 198.245.254.124
---------------------------------------------------------------------------

==============================Original Headers==============================
16, 11 -- From zaluzec-at-microscopy.com Mon Dec 7 19:16:34 2009
16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
16, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB81GWm9009354
16, 11 -- for {microscopy-at-microscopy.com} ; Mon, 7 Dec 2009 19:16:33 -0600
16, 11 -- Mime-Version: 1.0
16, 11 -- Message-Id: {p06240802c743594d2f4a-at-[206.69.208.22]}
16, 11 -- Date: Mon, 7 Dec 2009 19:16:31 -0600
16, 11 -- To: microscopy-at-microscopy.com
16, 11 -- From: lgsimmerman-at-gmail.com (by way of MicroscopyListserver)
16, 11 -- Subject: viaWWW: TEM-Help with Fading Slides
16, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 0010756u44c1922d-at-bridgestone.co.jp Mon Dec 7 22:20:21 2009
Return-Path: {0010756u44c1922d-at-bridgestone.co.jp}
Received: from [85.143.74.188] ([85.143.74.188])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB84JLqZ009788;
Mon, 7 Dec 2009 22:20:01 -0600
Received: from [23.172.103.99] (account a.kano-at-acekoeki.co.jp HELO uyrplmukxc.yvzikeupzrsbs.ua)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 278996120 for microscopylistserverarchive-at-microscopy.com; Tue, 8 Dec 2009 07:20:20 +0300

Lauren-
Cytoseal is a toluene-based acrylic medium. The toluene is
dissolving out the basic fuchsin. You may want to try a
water-soluble medium. I don't know of one to suggest off-hand, that
would be permanent. Perhaps you could leave the slides uncovered for
storage, and do a temporary water-based mount for examination &
photography.
Lee

}
} Email: lgsimmerman-at-gmail.com
} Name: Lauren Simmerman
}
} Organization: EM Labs, Inc.
}
} Title-Subject: [Filtered] TEM-Help with Fading Slides
}
} Question: Hi,
} It has been several years since I have posted a question, but since
} the member's answers helped me last time, I am hoping for the same
} result this time!
}
} We are a diagnostic pathology, private EM lab. A quick run down of
} our processing is: washing the sample in Sorenson's Phosphated
} Buffer, osmium tetroxide, water wash, dehydration series in acetone,
} Spurr Low Viscocity resin. The blocks cure overnight in a 65-70
} degree C oven.
}
} Our semi-thin sections are stained with Basic Fuchsin/Methylene
} Blue(~ 10 sec. with each stain on a 250 degree F hotplate). The
} staining pattern is similar to an H&E. The results are beautiful.
} Glycogen, collogen, & nuclei are blue with nucleoli, cytoplasm,
} mitochondria, & basal lamina bright pink.
}
} The problem became apparent about a year ago when we noticed that
} after cover-slipping with Cytoseal-60, a low viscocity mounting
} medium, the blue and pink faded, leaving the sections with a
} homogenous purple color.
}
} What would be the cause of the sections turning colors once
} cover-slipped? If left un-covered the color stays perfect.
} We have thought about going back to Toluidine Blue, but the
} pathologists prefer the results of the BFMB stain when it works
} properly.
}
} Thanks for any help with this.
}
} Lauren Simmerman
} EM Labs, Inc.
} Birmingham, AL
} 205-939-9741
}
}
}
}
}
} Login Host: 198.245.254.124
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 16, 11 -- From zaluzec-at-microscopy.com Mon Dec 7 19:16:34 2009
} 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 16, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id nB81GWm9009354
} 16, 11 -- for {microscopy-at-microscopy.com} ; Mon, 7 Dec 2009 19:16:33 -0600
} 16, 11 -- Mime-Version: 1.0
} 16, 11 -- Message-Id: {p06240802c743594d2f4a-at-[206.69.208.22]}
} 16, 11 -- Date: Mon, 7 Dec 2009 19:16:31 -0600
} 16, 11 -- To: microscopy-at-microscopy.com
} 16, 11 -- From: lgsimmerman-at-gmail.com (by way of MicroscopyListserver)
} 16, 11 -- Subject: viaWWW: TEM-Help with Fading Slides
} 16, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================


--
Lee Cohen-Gould, M.S.
Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology
and Optical Microscopy Core Facilities
Weill Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
5, 35 -- From lcgould-at-med.cornell.edu Tue Dec 8 08:26:44 2009
5, 35 -- Received: from mail-gw2.med.cornell.edu (mail-gw2.med.cornell.edu [140.251.3.2])
5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB8EQiqO001824
5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 08:26:44 -0600
5, 35 -- MIME-version: 1.0
5, 35 -- Content-transfer-encoding: 7BIT
5, 35 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed
5, 35 -- Received: from mpx4.med.cornell.edu ([140.251.11.120])
5, 35 -- by mail-gw2.med.cornell.edu
5, 35 -- (Sun Java(tm) System Messaging Server 6.3-8.03 (built Apr 24 2009; 32bit))
5, 35 -- with ESMTP id {0KUC0056W84KJJD0-at-mail-gw2.med.cornell.edu} for
5, 35 -- microscopy-at-microscopy.com; Tue, 08 Dec 2009 09:26:44 -0500 (EST)
5, 35 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
5, 35 -- by mpx4.med.cornell.edu
5, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.04 64bit (built Jul 2 2009))
5, 35 -- with ESMTPA id {0KUC0025384H7C00-at-mpx4.med.cornell.edu} ; Tue,
5, 35 -- 08 Dec 2009 09:26:44 -0500 (EST)
5, 35 -- X-PMX-Version: 5.5.8.383112, Antispam-Engine: 2.7.2.376379,
5, 35 -- Antispam-Data: 2009.12.8.141530
5, 35 -- X-Perlmx-Spam: Gauge=X, , Probability=10%, Report=' TO_IN_SUBJECT 0.5,
5, 35 -- BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0,
5, 35 -- TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0,
5, 35 -- __C230066_P2 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0,
5, 35 -- __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0,
5, 35 -- __HAS_MSGID 0, __MEDS_PLAIN 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0,
5, 35 -- __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0,
5, 35 -- __URI_NS '
5, 35 -- Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
5, 35 -- Message-id: {p06230908c74411c15b2b-at-[140.251.48.23]}
5, 35 -- In-reply-to: {200912080126.nB81QT0j003275-at-ns.microscopy.com}
5, 35 -- References: {200912080126.nB81QT0j003275-at-ns.microscopy.com}
5, 35 -- Date: Tue, 08 Dec 2009 09:26:38 -0500
5, 35 -- To: lgsimmerman-at-gmail.com, Microscopy Listserver {microscopy-at-microscopy.com}
5, 35 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
5, 35 -- Subject: Re: [Microscopy] viaWWW: TEM-Help with Fading Slides
==============================End of - Headers==============================

From account2-at-ms21.hinet.net Tue Dec 8 12:29:44 2009
Return-Path: {account2-at-ms21.hinet.net}
Received: from host-89-231-240-142.mielec.mm.pl (host-89-231-240-142.mielec.mm.pl [89.231.240.142])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB8ISiqJ023949;
Tue, 8 Dec 2009 12:29:23 -0600
Received: from 89.231.240.142 by ms21a.hinet.net; Tue, 8 Dec 2009 19:29:43 +0100

We have a Lieca UC6 with a key pad controller. The north south knife approach is not moving with either the track wheel or the step buttons. When I turn the microtome on, the little box on the key pad controller display indicates that the approach is fully south. Then I push the north step or turn the track wheel north, and the the knife does not appear to move at all. Then the approach box on the display shows both arrows, that is the approach is simultaneously at its south and north limit. I have looked for controls that can adjust the approach window, but have found none. I have turned turned the unit off and on several times hoping that it would reset. One time I did get a little north and south movement (about 3 micrometers, that is I could push the the step button about 3 times) but it quickly stopped moving. If anyone can give me some insight as to what is going on and how to fix it, your help would be greatly appreciated. We do not have the microtomes on a service contract so I need to try every thing possible before I call Leica for service
Bob
Dr. Robert J. Schmitz
Associate Professor of Anatomical Sciences
Department of Biology
University of Wisconsin-Stevens Point
800 Reserve Street, 380 TNR Bld
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://www.uwsp.edu/biology/faculty/rschmitz/index.html


==============================Original Headers==============================
2, 27 -- From rschmitz-at-uwsp.edu Tue Dec 8 16:01:47 2009
2, 27 -- Received: from emsmx1.uwsp.edu (emsmx1.uwsp.edu [143.236.32.81])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB8M1lFF002220
2, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 16:01:47 -0600
2, 27 -- Received: from EMSHUB1.uwsp.edu ([143.236.37.76]) by emsmx1.uwsp.edu with Microsoft SMTPSVC(6.0.3790.3959);
2, 27 -- Tue, 8 Dec 2009 16:01:43 -0600
2, 27 -- Received: from EMS7.uwsp.edu ([143.236.37.207]) by emshub1.uwsp.edu
2, 27 -- ([143.236.37.76]) with mapi; Tue, 8 Dec 2009 16:00:51 -0600
2, 27 -- From: "Schmitz, Robert" {rschmitz-at-uwsp.edu}
2, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
2, 27 -- Date: Tue, 8 Dec 2009 16:01:44 -0600
2, 27 -- Subject: LIECA UC6 knife approach problem
2, 27 -- Thread-Topic: LIECA UC6 knife approach problem
2, 27 -- Thread-Index: Acp4Ugdij97BTqgtAUCRDn0WTpJ6BQ==
2, 27 -- Message-ID: {C74428E9.793F%rschmitz-at-uwsp.edu}
2, 27 -- Accept-Language: en-US
2, 27 -- Content-Language: en
2, 27 -- X-MS-Has-Attach:
2, 27 -- X-MS-TNEF-Correlator:
2, 27 -- x-ninja-pim: Scanned by Ninja
2, 27 -- x-ninja-attachmentfiltering: (no action)
2, 27 -- acceptlanguage: en-US
2, 27 -- Content-Type: text/plain; charset="iso-8859-1"
2, 27 -- MIME-Version: 1.0
2, 27 -- X-OriginalArrivalTime: 08 Dec 2009 22:01:43.0464 (UTC) FILETIME=[07104A80:01CA7852]
2, 27 -- Content-Transfer-Encoding: 8bit
2, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB8M1lFF002220
==============================End of - Headers==============================

From 30006-at-adamjeeinsurance.com Tue Dec 8 16:59:47 2009
Return-Path: {30006-at-adamjeeinsurance.com}
Received: from 190-29-190-28.une.net.co (adsl190-29-190-28.une.net.co [190.29.190.28] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB8MxjrF019670;
Tue, 8 Dec 2009 16:59:46 -0600
Received: from [129.113.99.116] (helo=bqwwu.koguweelka.info)
by 190-29-190-28.une.net.co with esmtpa (Exim 4.69)
(envelope-from )
id 1MM2UT-3247he-EN
for microscopylistserverarchive-at-microscopy.com; Tue, 8 Dec 2009 23:59:45 +0100

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rschmitz-at-uwsp.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rschmitz-at-uwsp.edu
Name: Robert Schmitz

Organization: University of Wisconsin Stevens Point

Title-Subject: [Filtered] LIECA UC6 knife approach problem

Question: We have a Lieca UC6 with a key pad controller. The north
south knife approach is not moving with either the track wheel or the
step buttons. When I turn the microtome on, the little box on the key
pad controller display indicates that the approach is fully south.
Then I push the north step or turn the track wheel north, and the the
knife does not appear to move at all. Then the approach box on the
display shows both arrows, that is the approach is simutaneously at
its south and north limit. I have looked for controls that can
adjust the approach window, but have found none. I have turned
turned the unit off and on several times hoping that it would reset.
One time I did get a little north and south movement (about 3
micrometers, that is I could push the the step button about 3 times)
but it quickly stopped moving.

If anyone can give me some insight as to what is going on and how to
fix it, your help would be greatly appreciated. We do not have the
microtomes on a service contract so I need to try every thing
possible before I call Leica for service

Bob Schmitz
Dept of Biology, UWSP
Stevens Point WI
rschmitz-at-uwsp.edu


Login Host: 143.236.32.5
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Dec 8 18:10:06 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB90A61M022681
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 18:10:06 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c7449aea9188-at-[206.69.208.22]}
9, 11 -- Date: Tue, 8 Dec 2009 18:08:29 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: rschmitz-at-uwsp.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: LIECA UC6 knife approach problem
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: roger.craig-at-umassmed.edu
Date: Tue, 8 Dec 2009 18:10:09 -0600
Subject: [Microscopy] viaWWW: TEM technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both roger.craig-at-umassmed.edu as
well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: roger.craig-at-umassmed.edu
Name: Roger Craig

Organization: University of Massachusetts Medical School

Title-Subject: [Filtered] TEM technician position

Question: EM technician position available in
laboratory of Dr. Roger Craig, University of
Massachusetts Medical School, Worcester,
Massachusetts. Basic research on ultrastructure
of smooth muscle. The position involves serial
thin sectioning and TEM imaging of sections.
Could suit an experienced
microtomist-microscopist or a beginner with good
manual skills and willing to learn the
techniques. Part-time is an option. Available
immediately. Applicants should have a bachelorís
degree in biological sciences, and preferably one
or more years of lab experience. Equipment
includes Leica U7 ultramicrotome, diamond knives,
Leica Trim 2, 3 TEMs, 1 SEM, digital cameras.
For more information, contact Roger Craig
(roger.craig-at-umassmed.edu; 508 856 2474).

Login Host: 146.189.245.215
---------------------------------------------------------------------------


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Tue Dec 8 18:10:08 2009
7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB90A61O022681
7, 13 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 18:10:07 -0600
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06240802c7449b129af8-at-[206.69.208.22]}
7, 13 -- Date: Tue, 8 Dec 2009 18:09:07 -0600
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: roger.craig-at-umassmed.edu (by way of MicroscopyListserver)
7, 13 -- Subject: viaWWW: TEM technician position
7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
7, 13 -- Content-Transfer-Encoding: 8bit
7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB90A61O022681
==============================End of - Headers==============================




From: bill.mcmanus-at-usu.edu
Date: Tue, 8 Dec 2009 18:35:36 -0600
Subject: [Microscopy] viaWWW: critical point dryer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bill.mcmanus-at-usu.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bill.mcmanus-at-usu.edu
Name: Bill McManus

Organization: Western Dairy Center, Utah State University

Title-Subject: [Filtered] critical point dryer

Question: We have gotten our old Hitachi 4000 running, but a lot of
the equipment has disappeared. We need a CPD. If any one has an old
one they do not need or have any leads, please let me know.

Bill

Login Host: 129.123.249.177
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Dec 8 18:35:36 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB90ZZA9020261
7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 18:35:36 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240800c744a1470f6b-at-[206.69.208.22]}
7, 11 -- Date: Tue, 8 Dec 2009 18:35:35 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: bill.mcmanus-at-usu.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: critical point dryer
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: lisa-at-glosuntech.com
Date: Wed, 9 Dec 2009 00:13:44 -0600
Subject: [Microscopy] Hitachi H600 TEM Parts needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues....

Just performing a system test. I've had a few people mention
they had problems posting and I'm checking to see if it is
a problem with the Email filters. Or if it is elsewhere.

You may safely disregard this message.

Nestor
Your Friendly Neighborhood SysOp.



==============================Original Headers==============================
6, 17 -- From zaluzec-at-aaem.amc.anl.gov Tue Dec 8 20:15:14 2009
6, 17 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB92FDbE005223
6, 17 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 20:15:14 -0600
6, 17 -- Received: from [206.69.208.22] (msdvpn005.msd.anl.gov [130.202.251.5])
6, 17 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id nB92FDnu015652;
6, 17 -- Tue, 8 Dec 2009 20:15:13 -0600
6, 17 -- Message-Id: {971B5326-574A-4A51-83DB-B3C170A335E5-at-aaem.amc.anl.gov}
6, 17 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
6, 17 -- To: microscopy-at-microscopy.com
6, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
6, 17 -- Content-Transfer-Encoding: 7bit
6, 17 -- Mime-Version: 1.0 (Apple Message framework v936)
6, 17 -- Subject: Administrivia: Testing Server - You may ignore this message
6, 17 -- Date: Tue, 8 Dec 2009 20:15:12 -0600
6, 17 -- Cc: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
6, 17 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================

From anchovy-at-ms66.hinet.net Tue Dec 8 20:26:48 2009
Return-Path: {anchovy-at-ms66.hinet.net}
Received: from CABLEHOGAR.CL (114-221-201.DSL.CABLEHOGAR.CL [201.221.114.92] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB92Qe9w019939;
Tue, 8 Dec 2009 20:26:44 -0600
Received: from [35.150.79.9] (account a_juergens-at-adamjeeinsurance.com HELO adebpnsnt.rcytanbpcf.ua)
by CABLEHOGAR.CL (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 976445050 for microscopylistserverarchive-at-microscopy.com; Tue, 8 Dec 2009 23:26:43 -0300

Dear Members,

We are looking for parts for our H600 TEM. If you have any used or unused Hitachi H600 parts, please email us, we will be glad to pay small cost plus shipping for it.


Thank you.


Lisa

Glosuntech

==============================Original Headers==============================
7, 30 -- From lisa-at-glosuntech.com Wed Dec 9 00:13:44 2009
7, 30 -- Received: from mta4.brinkster.com (mta4.brinkster.com [65.182.109.83])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB96Dimn027486
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Dec 2009 00:13:44 -0600
7, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1])
7, 30 -- by mta4.brinkster.com (Postfix) with ESMTP id 4CD0D280E16
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Dec 2009 01:13:38 -0500 (EST)
7, 30 -- X-Virus-Scanned: amavisd-new at
7, 30 -- X-Spam-Flag: NO
7, 30 -- X-Spam-Score: 0.101
7, 30 -- X-Spam-Level:
7, 30 -- X-Spam-Status: No, score=0.101 tagged_above=-10 required=5
7, 30 -- tests=[BAYES_50=0.001, RDNS_NONE=0.1]
7, 30 -- Received: from mta4.brinkster.com ([127.0.0.1])
7, 30 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024)
7, 30 -- with ESMTP id 9Yz0JvO7xUSo for {Microscopy-at-Microscopy.Com} ;
7, 30 -- Wed, 9 Dec 2009 01:13:32 -0500 (EST)
7, 30 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217])
7, 30 -- by mta4.brinkster.com (Postfix) with ESMTP id 7ABBC280DE8
7, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Dec 2009 01:13:32 -0500 (EST)
7, 30 -- Date: Wed, 9 Dec 2009 01:13:30 -0500 (EST)
7, 30 -- From: lisa-at-glosuntech.com
7, 30 -- To: Microscopy-at-Microscopy.Com
7, 30 -- Message-ID: {32283686.966651260339210653.JavaMail.root-at-mail10a.brinkster.com}
7, 30 -- Subject: Hitachi H600 TEM Parts needed
7, 30 -- MIME-Version: 1.0
7, 30 -- Content-Type: text/plain; charset=utf-8
7, 30 -- Content-Transfer-Encoding: 7bit
7, 30 -- X-Originating-IP: [75.140.102.193]
7, 30 -- X-Mailer: Zimbra 5.0.8_GA_2463.RHEL4 (ZimbraWebClient - IE7 (Win)/5.0.8_GA_2462.RHEL4_64)
==============================End of - Headers==============================




From: gillian.smith-at-abdn.ac.uk
Date: Wed, 9 Dec 2009 03:38:37 -0600
Subject: [Microscopy] viaWWW: LIECA UC6 knife approach problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've had this problem before with our UC6. It occured over the Christmas period when it had been out of use for a couple of weeks. Unfortunately I don't think there's anything you could do, we had to get Leica out. The mechanism within the UC6 had seized.

Sorry I can't give you more information.


Gillian

Senior Histology Technician
Histology and EM Facility
University of Aberdeen
Institute of Medical Sciences
Foresterhill
Aberdeen
Scotland

www.abdn.ac.uk/ims/histology
________________________________________
X-from: rschmitz-at-uwsp.edu [rschmitz-at-uwsp.edu]
Sent: 09 December 2009 00:20
To: Smith, Gillian C.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rschmitz-at-uwsp.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rschmitz-at-uwsp.edu
Name: Robert Schmitz

Organization: University of Wisconsin Stevens Point

Title-Subject: [Filtered] LIECA UC6 knife approach problem

Question: We have a Lieca UC6 with a key pad controller. The north
south knife approach is not moving with either the track wheel or the
step buttons. When I turn the microtome on, the little box on the key
pad controller display indicates that the approach is fully south.
Then I push the north step or turn the track wheel north, and the the
knife does not appear to move at all. Then the approach box on the
display shows both arrows, that is the approach is simutaneously at
its south and north limit. I have looked for controls that can
adjust the approach window, but have found none. I have turned
turned the unit off and on several times hoping that it would reset.
One time I did get a little north and south movement (about 3
micrometers, that is I could push the the step button about 3 times)
but it quickly stopped moving.

If anyone can give me some insight as to what is going on and how to
fix it, your help would be greatly appreciated. We do not have the
microtomes on a service contract so I need to try every thing
possible before I call Leica for service

Bob Schmitz
Dept of Biology, UWSP
Stevens Point WI
rschmitz-at-uwsp.edu


Login Host: 143.236.32.5
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Tue Dec 8 18:10:06 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB90A61M022681
9, 11 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2009 18:10:06 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c7449aea9188-at-[206.69.208.22]}
9, 11 -- Date: Tue, 8 Dec 2009 18:08:29 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: rschmitz-at-uwsp.edu (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: LIECA UC6 knife approach problem
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


The University of Aberdeen is a charity registered in Scotland, No SC013683.


==============================Original Headers==============================
21, 31 -- From gillian.smith-at-abdn.ac.uk Wed Dec 9 03:38:36 2009
21, 31 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.8])
21, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB99casq017803
21, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 03:38:36 -0600
21, 31 -- Received: from ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83] helo=mail.abdn.ac.uk)
21, 31 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.69)
21, 31 -- (envelope-from {gillian.smith-at-abdn.ac.uk} )
21, 31 -- id 1NIJ0D-0005cc-9Y; Wed, 09 Dec 2009 09:38:33 +0000
21, 31 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.82]) by
21, 31 -- ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83]) with mapi; Wed, 9 Dec 2009
21, 31 -- 09:37:43 +0000
21, 31 -- From: "Smith, Gillian C." {gillian.smith-at-abdn.ac.uk}
21, 31 -- To: "rschmitz-at-uwsp.edu" {rschmitz-at-uwsp.edu} ,
21, 31 -- "microscopy-at-microscopy.com"
21, 31 -- {microscopy-at-microscopy.com}
21, 31 -- Date: Wed, 9 Dec 2009 09:33:32 +0000
21, 31 -- Subject: RE: [Microscopy] viaWWW: LIECA UC6 knife approach problem
21, 31 -- Thread-Topic: [Microscopy] viaWWW: LIECA UC6 knife approach problem
21, 31 -- Thread-Index: Acp4ZXdNUQZ+cAVZTiG9ZqWQ9Tn+VgATTUxk
21, 31 -- Message-ID: {048C5AF41A475C43B33E06886BD3B341C3EFC7B860-at-VMAILB.uoa.abdn.ac.uk}
21, 31 -- References: {200912090020.nB90KdOK017835-at-ns.microscopy.com}
21, 31 -- In-Reply-To: {200912090020.nB90KdOK017835-at-ns.microscopy.com}
21, 31 -- Accept-Language: en-US
21, 31 -- Content-Language: en-GB
21, 31 -- X-MS-Has-Attach:
21, 31 -- X-MS-TNEF-Correlator:
21, 31 -- acceptlanguage: en-US
21, 31 -- Content-Type: text/plain; charset="us-ascii"
21, 31 -- MIME-Version: 1.0
21, 31 -- Content-Transfer-Encoding: 8bit
21, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB99casq017803
==============================End of - Headers==============================




From: brantnerc-at-nbacc.net
Date: Wed, 9 Dec 2009 07:00:40 -0600
Subject: [Microscopy] LIECA UC6 knife approach problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning Bob from UW-SP from a UW-Mil grad,
I had a problem with the Leica UC6 and movement of its knifeholder a
year or so ago. I recall that I fixed it by pushing some buttons. So
here is what I think I did. From the touch screen go into the "menu" at
the bottom of the page. Then go into the "setup" screen. From there
either the "Reset Feed, Center Stage" or the "Reset to Factory Settings"
cleared up the problem. I think that the microtome I was using just
lost its place and needed to be told where the middle of its home base
was. I hope this helps.
Chris

Christine A. Brantner, PhD
Electron Microscopy Manager
BNBI/NBACC
110 Thomas Johnson Dr., Suite 300
Frederick, MD 21702
301-620-1655 desk
301-957-0320 mobile

-----Original Message-----
X-from: rschmitz-at-uwsp.edu [mailto:rschmitz-at-uwsp.edu]
Sent: Tuesday, December 08, 2009 5:13 PM
To: Brantner, Christine A

We have a Lieca UC6 with a key pad controller. The north south knife
approach is not moving with either the track wheel or the step buttons.
When I turn the microtome on, the little box on the key pad controller
display indicates that the approach is fully south. Then I push the
north step or turn the track wheel north, and the the knife does not
appear to move at all. Then the approach box on the display shows both
arrows, that is the approach is simultaneously at its south and north
limit. I have looked for controls that can adjust the approach window,
but have found none. I have turned turned the unit off and on several
times hoping that it would reset. One time I did get a little north and
south movement (about 3 micrometers, that is I could push the the step
button about 3 times) but it quickly stopped moving. If anyone can give
me some insight as to what is going on and how to fix it, your help
would be greatly appreciated. We do not have the microtomes on a service
co!
ntract so I need to try every thing possible before I call Leica for
service
Bob
Dr. Robert J. Schmitz
Associate Professor of Anatomical Sciences
Department of Biology
University of Wisconsin-Stevens Point
800 Reserve Street, 380 TNR Bld
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://www.uwsp.edu/biology/faculty/rschmitz/index.html


==============================Original
Headers==============================
2, 27 -- From rschmitz-at-uwsp.edu Tue Dec 8 16:01:47 2009
2, 27 -- Received: from emsmx1.uwsp.edu (emsmx1.uwsp.edu
[143.236.32.81])
2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id nB8M1lFF002220
2, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Dec 2009
16:01:47 -0600
2, 27 -- Received: from EMSHUB1.uwsp.edu ([143.236.37.76]) by
emsmx1.uwsp.edu with Microsoft SMTPSVC(6.0.3790.3959);
2, 27 -- Tue, 8 Dec 2009 16:01:43 -0600
2, 27 -- Received: from EMS7.uwsp.edu ([143.236.37.207]) by
emshub1.uwsp.edu
2, 27 -- ([143.236.37.76]) with mapi; Tue, 8 Dec 2009 16:00:51 -0600
2, 27 -- From: "Schmitz, Robert" {rschmitz-at-uwsp.edu}
2, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
2, 27 -- Date: Tue, 8 Dec 2009 16:01:44 -0600
2, 27 -- Subject: LIECA UC6 knife approach problem
2, 27 -- Thread-Topic: LIECA UC6 knife approach problem
2, 27 -- Thread-Index: Acp4Ugdij97BTqgtAUCRDn0WTpJ6BQ==
2, 27 -- Message-ID: {C74428E9.793F%rschmitz-at-uwsp.edu}
2, 27 -- Accept-Language: en-US
2, 27 -- Content-Language: en
2, 27 -- X-MS-Has-Attach:
2, 27 -- X-MS-TNEF-Correlator:
2, 27 -- x-ninja-pim: Scanned by Ninja
2, 27 -- x-ninja-attachmentfiltering: (no action)
2, 27 -- acceptlanguage: en-US
2, 27 -- Content-Type: text/plain; charset="iso-8859-1"
2, 27 -- MIME-Version: 1.0
2, 27 -- X-OriginalArrivalTime: 08 Dec 2009 22:01:43.0464 (UTC)
FILETIME=[07104A80:01CA7852]
2, 27 -- Content-Transfer-Encoding: 8bit
2, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nB8M1lFF002220
==============================End of -
Headers==============================


==============================Original Headers==============================
10, 22 -- From brantnerc-at-nbacc.net Wed Dec 9 07:00:39 2009
10, 22 -- Received: from NBACC-MX1.NBACC.NET (nbacc-mx.nbacc.net [12.198.101.12])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9D0cBg016052
10, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 07:00:39 -0600
10, 22 -- Received: from ashburn-ex1.nbacc.net ([10.0.1.17])
10, 22 -- by TJ0-NBACC-EF1.NBACC.NET with ESMTP; 09 Dec 2009 08:00:38 -0500
10, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
10, 22 -- Content-class: urn:content-classes:message
10, 22 -- MIME-Version: 1.0
10, 22 -- Content-Type: text/plain;
10, 22 -- charset="us-ascii"
10, 22 -- Subject: FW: [Microscopy] LIECA UC6 knife approach problem
10, 22 -- Date: Wed, 9 Dec 2009 08:00:15 -0500
10, 22 -- Message-ID: {8AD0F53BCA525D4796320B6C1D322C2B0464A273-at-Ashburn-EX1.nbacc.net}
10, 22 -- X-MS-Has-Attach:
10, 22 -- X-MS-TNEF-Correlator:
10, 22 -- Thread-Topic: [Microscopy] LIECA UC6 knife approach problem
10, 22 -- Thread-Index: Acp4U5Zgn7e8XdccTGWSiUl8Jr9xBwAesmBg
10, 22 -- From: "Brantner, Christine A" {brantnerc-at-nbacc.net}
10, 22 -- To: {Microscopy-at-microscopy.com}
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9D0cBg016052
==============================End of - Headers==============================




From: gary.nichols-at-pfizer.com
Date: Wed, 9 Dec 2009 08:19:27 -0600
Subject: [Microscopy] viaWWW: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gary.nichols-at-pfizer.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gary.nichols-at-pfizer.com
Name: Gary Nichols

Title-Subject: [Filtered] Calculation of average atomic number

Question: Dear Listers,

When observing backscattered electron images, the relative brightness
of objects, such as particles, is (we are lead to believe) a function
of the average atomic number(av.Z), so that bright objects have a
high av.Z relative to darker ones having a lower av.Z. This is taken
as read by all users of BS detectors and is a well known phenomenon
that is described in books and papers. What I would like to know is
how to calculate the av.Z for a material (e.g., a mineral, an alloy
or an organic compound). I don't like to assume that specimens I am
viewing have a high or low av.Z just by using the relative brightness
between objects.

Thank you.
Gary



Login Host: 168.224.1.14
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:19:26 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9EJPk6001628
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 08:19:26 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c745625c513b-at-[206.69.208.22]}
9, 11 -- Date: Wed, 9 Dec 2009 08:19:24 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: gary.nichols-at-pfizer.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Calculation of average atomic number
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jsb43-at-cam.ac.uk
Date: Wed, 9 Dec 2009 10:10:52 -0600
Subject: [Microscopy] Re: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pal.baggethun-at-elkem.no as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pal.baggethun-at-elkem.no
Name: P. Baggethun

Organization: Elkem Solar Research

Title-Subject: [Filtered] LM: reflectance standards

Question: Does anyone know where to search for a reflectance standard
for light microscopy with reflectance close to 1.0% and bireflectance
virtually zero for normally incident light at 546 nm wavelength in
immersion oil of refractive index 1.5180?

Login Host: 212.4.33.171
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:20:00 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9EJw4j002227
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 08:20:00 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240801c745627e5950-at-[206.69.208.22]}
6, 11 -- Date: Wed, 9 Dec 2009 08:19:58 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: pal.baggethun-at-elkem.no (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: LM: reflectance standards
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 3dsong-at-tbtt.th.com Wed Dec 9 09:36:44 2009
Return-Path: {3dsong-at-tbtt.th.com}
Received: from [190.209.98.171] ([190.209.98.171])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9Fafod003183;
Wed, 9 Dec 2009 09:36:43 -0600
Received: from [99.18.187.108] (helo=nsukoa.ocjvjkvl.ua)
by with esmtpa (Exim 4.69)
(envelope-from )
id 1MM933-3294je-5Q
for microscopylistserverarchive-at-microscopy.com; Wed, 9 Dec 2009 11:36:42 -0400
Message-ID: {2846129592.HOW83P5S343695-at-oelcquwgidwrjfm.swbxkvojdoao.info}

Dear Gary,

According to Joseph Goldstein and his co-workers ("SEM and X-ray
microanalysis" 3rd edition, Kluwer Academic/Plenum Publishers), the
backscatter coefficient of a compound material should be calculated from
the elemental coefficients weighted by the weight (or mass) concentrations
*not* the atomic concentrations. See section 3.4.1 on page 77 of this
edition.

Goldstein et al make reference to the work of Heinrich for this statement:
Heinrich K.F.J. (1966) In Proceedings of the 4th International Conference
on X-ray Optics and Microanalysis (Editoes: Castaing, Deschamps and
Philibert) Herman, Paris page 159.

Best wishes, Jon

} On Dec 9 2009, gary.nichols-at-pfizer.com wrote:
} Email: gary.nichols-at-pfizer.com
} Name: Gary Nichols
}
} Title-Subject: [Filtered] Calculation of average atomic number
}
} Question: Dear Listers,
}
} When observing backscattered electron images, the relative brightness
} of objects, such as particles, is (we are lead to believe) a function
} of the average atomic number(av.Z), so that bright objects have a
} high av.Z relative to darker ones having a lower av.Z. This is taken
} as read by all users of BS detectors and is a well known phenomenon
} that is described in books and papers. What I would like to know is
} how to calculate the av.Z for a material (e.g., a mineral, an alloy
} or an organic compound). I don't like to assume that specimens I am
} viewing have a high or low av.Z just by using the relative brightness
} between objects.
}
} Thank you.
} Gary


==============================Original Headers==============================
6, 28 -- From jsb43-at-hermes.cam.ac.uk Wed Dec 9 10:10:52 2009
6, 28 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131])
6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9GAp09004265
6, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 10:10:52 -0600
6, 28 -- X-Cam-AntiVirus: no malware found
6, 28 -- X-Cam-SpamDetails: not scanned
6, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
6, 28 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:37377)
6, 28 -- by ppsw-1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:25)
6, 28 -- with esmtpa (EXTERNAL:jsb43) id 1NIP7r-00033X-4O (Exim 4.70)
6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Wed, 09 Dec 2009 16:10:51 +0000
6, 28 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk)
6, 28 -- with local (PRAYER:jsb43) id 1NIP7r-0003lR-BH (Exim 4.67)
6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Wed, 09 Dec 2009 16:10:51 +0000
6, 28 -- Received: from [131.111.102.18] by webmail.hermes.cam.ac.uk
6, 28 -- with HTTP (Prayer-1.3.2); 09 Dec 2009 16:10:51 +0000
6, 28 -- Date: 09 Dec 2009 16:10:51 +0000
6, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk}
6, 28 -- To: gary.nichols-at-pfizer.com
6, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com}
6, 28 -- Subject: Re: Calculation of average atomic number
6, 28 -- Message-ID: {Prayer.1.3.2.0912091610510.6924-at-hermes-1.csi.cam.ac.uk}
6, 28 -- In-Reply-To: {200912091427.nB9ERSNu023424-at-ns.microscopy.com}
6, 28 -- References: {200912091427.nB9ERSNu023424-at-ns.microscopy.com}
6, 28 -- X-Mailer: Prayer v1.3.2
6, 28 -- Mime-Version: 1.0
6, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1
6, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk}
==============================End of - Headers==============================




From: edelmare-at-muohio.edu
Date: Wed, 9 Dec 2009 10:41:11 -0600
Subject: [Microscopy] Re: air table gas consumption

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jim:

We have four kinetics systems tables (36" x 40") with confocal or
light microscopes on them. We use pre-purified nitrogen 300cu-ft
tanks on them (we use ppN2 for all our scope and table needs), and we
leave the tables active (filled) 24/7. With regular (30-50 hrs/wk
use) we swap out tanks 1.5 - 2.5 years. So $9 for two-years.




On 19 Nov 2009 at 11:15, jehrman-at-mta.ca wrote:

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Good day listers,
}
} We've just had a Kinetic air table delivered for our new Zeiss Apotome
} scope and I'm casting about for a source of air for the table. Anybody
} out there have an idea how much gas is consumed by such a table. How
} often (roughly) would we need to change a standard nitrogen cylinder?
} Alternatively, if we provided air with a compressor, how often could
} we expect the compressor to cycle in the course of a day? We're mainly
} concerned with noise, and the compressor provided by the vendor seems
} a little expensive. Can we get away with a less expensive solution?
}
} As usual, thanks in advance,
}
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/dmf
}
} Svent-Gyorgyi's Axiom:
} Discovery consists of seeing what everybody
} has seen and thinking what nobody thought.
}
}
} ==============================Original
} Headers============================== 10, 18 -- From jehrman-at-mta.ca
} Thu Nov 19 10:14:11 2009 10, 18 -- Received: from mailgate1.mta.ca
} (mailgate1.mta.ca [138.73.1.208]) 10, 18 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id nAJGEBdM008974 10, 18 -- for
} {Microscopy-at-microscopy.com} ; Thu, 19 Nov 2009 10:14:11 -0600 10, 18 --
} Received: from host-22-194.mta.ca ([138.73.22.194]:63368) 10, 18 --
} by mailgate1.mta.ca (smtp.mta.ca [138.73.1.137]:25) 10, 18 -- with
} esmtp id 1NB9e6-00030J-CB (Exim 4.69) for Microscopy-at-microscopy.com
} 10, 18 -- (return-path {jehrman-at-mta.ca} ); Thu, 19 Nov 2009 12:14:10
} -0400 10, 18 -- Message-ID: {4B056E97.7040309-at-mta.ca} 10, 18 -- Date:
} Thu, 19 Nov 2009 12:13:11 -0400 10, 18 -- From: "James M. Ehrman"
} {jehrman-at-mta.ca} 10, 18 -- User-Agent: Thunderbird 2.0.0.23
} (Windows/20090812) 10, 18 -- MIME-Version: 1.0 10, 18 -- To:
} Microscopy Listserv {Microscopy-at-microscopy.com} 10, 18 -- Subject: air
} table gas consumption 10, 18 -- X-Enigmail-Version: 0.96.0 10, 18 --
} Content-Type: text/plain; charset=ISO-8859-1 10, 18 --
} Content-Transfer-Encoding: 7bit ==============================End of -
} Headers==============================


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


==============================Original Headers==============================
10, 25 -- From edelmare-at-muohio.edu Wed Dec 9 10:41:11 2009
10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.71])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9GfARZ019387
10, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 9 Dec 2009 10:41:11 -0600
10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10])
10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id nB9GfDQi024702;
10, 25 -- Wed, 9 Dec 2009 11:41:13 -0500
10, 25 -- Received: from [10.34.160.234] ([10.34.160.234])
10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id nB9GfAf0020946;
10, 25 -- Wed, 9 Dec 2009 11:41:10 -0500
10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
10, 25 -- To: "jehrman-at-mta.ca" {jehrman-at-mta.ca}
10, 25 -- Date: Wed, 09 Dec 2009 11:41:10 -0500
10, 25 -- MIME-Version: 1.0
10, 25 -- Subject: Re: [Microscopy] air table gas consumption
10, 25 -- CC: microscopy-at-Microscopy.com
10, 25 -- Message-ID: {4B1F8CD6.20484.E5C0B71-at-edelmare.muohio.edu}
10, 25 -- Priority: normal
10, 25 -- In-reply-to: {200911191615.nAJGFC9F009957-at-ns.microscopy.com}
10, 25 -- References: {200911191615.nAJGFC9F009957-at-ns.microscopy.com}
10, 25 -- X-mailer: Pegasus Mail for Windows (4.41)
10, 25 -- Content-type: text/plain; charset=US-ASCII
10, 25 -- Content-transfer-encoding: 7BIT
10, 25 -- Content-description: Mail message body
10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.71
==============================End of - Headers==============================




From: donovan-at-uoregon.edu
Date: Wed, 9 Dec 2009 10:42:58 -0600
Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
There is a simple approximate answer (used in most textbooks) and a
more complicated more accurate answer that really only matters for
quantitative analysis.

What it boils down to is that because A/Z is (very) roughly a
constant over the periodic table, a simple sum of the mass fractions
will give a surprisingly close answer (closer than one might expect
due to a serendipitous trend in A/Z that pushes the calculation in
the right direction for a reason unrelated to elastic scattering,
that is neutron count). However, because mass doesn't actually effect
elastic scattering of electrons (largest effect is with hydrogen with
optimum momentum exchange producing a 0.05% effect), the average Z
should be calculated by a function of proton (or electron) ratios
with a correction for screening of nuclear charge by inner electron orbitals).

The Penepma monte-carlo code produces a very accurate BSE calculation
but takes a few hours. Can be downloaded here:

http://epmalab.uoregon.edu/calczaf.htm

See papers linked here and let me know if you have any questions.

http://epmalab.uoregon.edu/published.htm

john

At 06:24 AM 12/9/2009, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
12, 21 -- From donovan-at-uoregon.edu Wed Dec 9 10:42:57 2009
12, 21 -- Received: from QMTA08.emeryville.ca.mail.comcast.net (qmta08.emeryville.ca.mail.comcast.net [76.96.30.80])
12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9GgveS023337
12, 21 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 10:42:57 -0600
12, 21 -- Message-Id: {200912091642.nB9GgveS023337-at-ns.microscopy.com}
12, 21 -- Received: from OMTA21.emeryville.ca.mail.comcast.net ([76.96.30.88])
12, 21 -- by QMTA08.emeryville.ca.mail.comcast.net with comcast
12, 21 -- id F4NT1d00a1u4NiLA84iwLG; Wed, 09 Dec 2009 16:42:57 +0000
12, 21 -- Received: from SOURCE.uoregon.edu ([67.160.163.204])
12, 21 -- by OMTA21.emeryville.ca.mail.comcast.net with comcast
12, 21 -- id F4ij1d0044Qundf8h4imlH; Wed, 09 Dec 2009 16:42:53 +0000
12, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
12, 21 -- Date: Wed, 09 Dec 2009 08:42:38 -0800
12, 21 -- To: gary.nichols-at-pfizer.com
12, 21 -- From: "John J. Donovan" {donovan-at-uoregon.edu}
12, 21 -- Subject: Re: [Microscopy] viaWWW: Calculation of average atomic number
12, 21 -- Cc: microscopy-at-microscopy.com
12, 21 -- In-Reply-To: {200912091424.nB9EOuJn016886-at-ns.microscopy.com}
12, 21 -- References: {200912091424.nB9EOuJn016886-at-ns.microscopy.com}
12, 21 -- Mime-Version: 1.0
12, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Wed, 9 Dec 2009 11:21:05 -0600
Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,

Simply use the chemical formula of the compound. Add all the Z numbers and divide by the total number of atoms in the formula. E.g. SiO2 = 14 + 8 + 8 =30 /3 = 10.
To large extent the actual composition and minor components are practically irrelevant since minor differences in average Z cannot be imaged easy by BSE. The contrast differences between the two phases you need to image need to be above 10% in order to be imaged successfully. Contrast differences above 1% but below 5% are very difficult to image and require significant efforts.

Monte-Carlo simulations would give adequate and precise answer but in most cases it is not worth the time to do the calculations.

For simple estimate of contrast you can use the formula:
BSE coeff = -0.0254 +0.016Z – 1.86x10-4Z^2 + 8.3x10-7Z^3
Contrast = (BSEcoeff 1 - BSE coeff 2)/ BSE coeff 2

Regards,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

phone 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu


On Dec 9, 2009, at 6:27 AM, gary.nichols-at-pfizer.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both gary.nichols-at-pfizer.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: gary.nichols-at-pfizer.com
} Name: Gary Nichols
}
} Title-Subject: [Filtered] Calculation of average atomic number
}
} Question: Dear Listers,
}
} When observing backscattered electron images, the relative brightness
} of objects, such as particles, is (we are lead to believe) a function
} of the average atomic number(av.Z), so that bright objects have a
} high av.Z relative to darker ones having a lower av.Z. This is taken
} as read by all users of BS detectors and is a well known phenomenon
} that is described in books and papers. What I would like to know is
} how to calculate the av.Z for a material (e.g., a mineral, an alloy
} or an organic compound). I don't like to assume that specimens I am
} viewing have a high or low av.Z just by using the relative brightness
} between objects.
}
} Thank you.
} Gary
}
}
}
} Login Host: 168.224.1.14
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:19:26 2009
} 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9EJPk6001628
} 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 08:19:26 -0600
} 9, 11 -- Mime-Version: 1.0
} 9, 11 -- Message-Id: {p06240800c745625c513b-at-[206.69.208.22]}
} 9, 11 -- Date: Wed, 9 Dec 2009 08:19:24 -0600
} 9, 11 -- To: microscopy-at-microscopy.com
} 9, 11 -- From: gary.nichols-at-pfizer.com (by way of MicroscopyListserver)
} 9, 11 -- Subject: viaWWW: Calculation of average atomic number
} 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================



==============================Original Headers==============================
12, 22 -- From bozhilov-at-ucr.edu Wed Dec 9 11:21:04 2009
12, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9HL4Fu016937
12, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 11:21:04 -0600
12, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
12, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
12, 22 -- with ESMTP id KDB94387;
12, 22 -- Wed, 9 Dec 2009 09:20:59 -0800 (PST)
12, 22 -- Subject: Re: [Microscopy] viaWWW: Calculation of average atomic number
12, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
12, 22 -- Content-Type: text/plain; charset=windows-1252
12, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
12, 22 -- In-Reply-To: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
12, 22 -- Date: Wed, 9 Dec 2009 09:21:01 -0800
12, 22 -- Cc: microscopy-at-microscopy.com
12, 22 -- Message-Id: {36B6A389-AEFE-4926-A110-8BEA86045199-at-ucr.edu}
12, 22 -- References: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
12, 22 -- To: gary.nichols-at-pfizer.com
12, 22 -- X-Mailer: Apple Mail (2.1077)
12, 22 -- X-Junkmail-Status: score=0/50, host=
12, 22 -- Content-Transfer-Encoding: 8bit
12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9HL4Fu016937
==============================End of - Headers==============================




From: DusevichV-at-umkc.edu
Date: Wed, 9 Dec 2009 12:24:46 -0600
Subject: [Microscopy] viaWWW: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Krassimir,

Average atomic number for BSE contrast should be calculated for
weight (not atomic) fractions, so
Z=w(1)*z1+w(2)*z2+...

Regards

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

} -----Original Message-----
} From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} Sent: Wednesday, December 09, 2009 11:22 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number
}
}
}
}
}
-----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} -----
}
} Gary,
}
} Simply use the chemical formula of the compound. Add all the Z numbers
} and divide by the total number of atoms in the formula. E.g. SiO2 = 14
} + 8 + 8 =30 /3 = 10.
} To large extent the actual composition and minor components are
} practically irrelevant since minor differences in average Z cannot be
} imaged easy by BSE. The contrast differences between the two phases
you
} need to image need to be above 10% in order to be imaged successfully.
} Contrast differences above 1% but below 5% are very difficult to image
} and require significant efforts.
}
} Monte-Carlo simulations would give adequate and precise answer but in
} most cases it is not worth the time to do the calculations.
}
} For simple estimate of contrast you can use the formula:
} BSE coeff = -0.0254 +0.016Z - 1.86x10-4Z^2 + 8.3x10-7Z^3
} Contrast = (BSEcoeff 1 - BSE coeff 2)/ BSE coeff 2
}
} Regards,
}
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} phone 951 827 2998
} fax 951 827 2489
} bozhilov-at-ucr.edu
}
}
} On Dec 9, 2009, at 6:27 AM, gary.nichols-at-pfizer.com wrote:
}
} }
} }
} }
} }
---------------------------------------------------------------------
} -------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
---------------------------------------------------------------------
} -------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} }
---------------------------------------------------------------------
} ------
} } Remember this posting is most likely not from a Subscriber, so when
} replying
} } please copy both gary.nichols-at-pfizer.com as well as the
} } MIcroscopy Listserver
} }
---------------------------------------------------------------------
} ------
} }
} } Email: gary.nichols-at-pfizer.com
} } Name: Gary Nichols
} }
} } Title-Subject: [Filtered] Calculation of average atomic number
} }
} } Question: Dear Listers,
} }
} } When observing backscattered electron images, the relative
brightness
} } of objects, such as particles, is (we are lead to believe) a
function
} } of the average atomic number(av.Z), so that bright objects have a
} } high av.Z relative to darker ones having a lower av.Z. This is
taken
} } as read by all users of BS detectors and is a well known phenomenon
} } that is described in books and papers. What I would like to know is
} } how to calculate the av.Z for a material (e.g., a mineral, an alloy
} } or an organic compound). I don't like to assume that specimens I am
} } viewing have a high or low av.Z just by using the relative
brightness
} } between objects.
} }
} } Thank you.
} } Gary
} }
} }
} }
} } Login Host: 168.224.1.14
} }
---------------------------------------------------------------------
} ------
} }
} } ==============================Original
} Headers==============================
} } 9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:19:26 2009
} } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP
} id nB9EJPk6001628
} } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009
08:19:26 -
} 0600
} } 9, 11 -- Mime-Version: 1.0
} } 9, 11 -- Message-Id: {p06240800c745625c513b-at-[206.69.208.22]}
} } 9, 11 -- Date: Wed, 9 Dec 2009 08:19:24 -0600
} } 9, 11 -- To: microscopy-at-microscopy.com
} } 9, 11 -- From: gary.nichols-at-pfizer.com (by way of
} MicroscopyListserver)
} } 9, 11 -- Subject: viaWWW: Calculation of average atomic number
} } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} } ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 12, 22 -- From bozhilov-at-ucr.edu Wed Dec 9 11:21:04 2009
} 12, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
} [138.23.226.212])
} 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP
} id nB9HL4Fu016937
} 12, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009
11:21:04 -
} 0600
} 12, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
} 12, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
} 12, 22 -- with ESMTP id KDB94387;
} 12, 22 -- Wed, 9 Dec 2009 09:20:59 -0800 (PST)
} 12, 22 -- Subject: Re: [Microscopy] viaWWW: Calculation of average
} atomic number
} 12, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
} 12, 22 -- Content-Type: text/plain; charset=windows-1252
} 12, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
} 12, 22 -- In-Reply-To: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
} 12, 22 -- Date: Wed, 9 Dec 2009 09:21:01 -0800
} 12, 22 -- Cc: microscopy-at-microscopy.com
} 12, 22 -- Message-Id: {36B6A389-AEFE-4926-A110-8BEA86045199-at-ucr.edu}
} 12, 22 -- References: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
} 12, 22 -- To: gary.nichols-at-pfizer.com
} 12, 22 -- X-Mailer: Apple Mail (2.1077)
} 12, 22 -- X-Junkmail-Status: score=0/50, host=
} 12, 22 -- Content-Transfer-Encoding: 8bit
} 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id nB9HL4Fu016937
} ==============================End of -
} Headers==============================



==============================Original Headers==============================
8, 25 -- From DusevichV-at-umkc.edu Wed Dec 9 12:24:46 2009
8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9IOkia000597
8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 12:24:46 -0600
8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
8, 25 -- Wed, 9 Dec 2009 12:24:45 -0600
8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
8, 25 -- Content-class: urn:content-classes:message
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain;
8, 25 -- charset="us-ascii"
8, 25 -- Subject: RE: [Microscopy] Re: viaWWW: Calculation of average atomic number
8, 25 -- Date: Wed, 9 Dec 2009 12:24:46 -0600
8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB30935FF5A-at-KC-MSX1.kc.umkc.edu}
8, 25 -- In-Reply-To: {200912091721.nB9HLalR017755-at-ns.microscopy.com}
8, 25 -- X-MS-Has-Attach:
8, 25 -- X-MS-TNEF-Correlator:
8, 25 -- thread-topic: [Microscopy] Re: viaWWW: Calculation of average atomic number
8, 25 -- thread-index: Acp49BD9XBWxYGohQlmSD3Yl/oDaJgACF9bg
8, 25 -- References: {200912091721.nB9HLalR017755-at-ns.microscopy.com}
8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
8, 25 -- To: {Microscopy-at-microscopy.com}
8, 25 -- X-OriginalArrivalTime: 09 Dec 2009 18:24:45.0452 (UTC) FILETIME=[E2230CC0:01CA78FC]
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9IOkia000597
==============================End of - Headers==============================




From: Jeff.Gschwend-at-hitachi-hta.com
Date: Wed, 9 Dec 2009 12:41:46 -0600
Subject: [Microscopy] Re: Albert Crewe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



For those on the list who knew Dr. Crewe, his daughter, Jennifer, has
provided the following information:

Memorial Service to Celebrate the Life of Albert V. Crewe
4 p.m. on February 20, 2010
Bond Chapel
On the campus of the University of Chicago, 1050 E. 59th Street
Reception immediately following at the Quadrangle Club, 1155 E. 57th
Street


In lieu of flowers, the family has established a trust fund for the
in-home health aides who cared for Dr. Crewe in the last year of his
life. Donations may be made to: Albert V. Crewe Caregivers Trust Chase
Bank, 106 Broadway, Chesterton, Indiana 46304


=========================================
Jeff Gschwend
Midwest Sales Manager
Hitachi High Technologies America, Inc.
Nanotechnology Systems Division
10 North Martingale Road, Suite 500
Schaumburg, IL 60173
Tel: 847.816.6098
Cell: 847.778.0143
Fax: 925-218-3231
e-mail: jeff.gschwend-at-hitachi-hta.com
Web site: www.hitachi-hta.com {http://www.hitachi-hta.com/}
=========================================

On Nov 22, 2009, at 10:11 AM, p.ingram-at-cellbio.duke.edu wrote:



------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html

------------------------------------------------------------------------
----

I am sure everyone will be saddened to learn of the passing
of Al Crewe on Wedesday Nov 18. He was an inspiration to all of
us
with his creativity and down-to-earth sense of humor. We send
condolences to his family and friends. He will be missed.

http://www.nytimes.com/2009/11/21/science/21crewe.html


--
Peter Ingram
Sr. Physicist
Adj. Asst. Professor of Pathology
Duke University Medical Center
PO Box 90319
DURHAM NC 27708-0319

Tel: 919 660-2695
Fax: 919 660-2671
Email: p.ingram-at-cellbio.duke.edu


==============================Original Headers==============================
13, 27 -- From prvs=5594a4edee=Jeff.Gschwend-at-hitachi-hta.com Wed Dec 9 12:41:25 2009
13, 27 -- Received: from mail1.hitachi.net (mail1.hitachi.net [128.241.23.75])
13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9IfP0P015508
13, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 12:41:25 -0600
13, 27 -- Received: from sjc-smtp01.hal.hitachi.com (sjc-hal-smtp01.hal.hitachi.local [137.168.7.184])
13, 27 -- by mail1.hitachi.net (8.13.8+Sun/8.13.7) with ESMTP id nB9IfPtO011384
13, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 10:41:25 -0800 (PST)
13, 27 -- X-AuditID: 89a8077b-b7cafae000000faf-38-4b1fef52cee1
13, 27 -- Received: from exchange.hitachi-hta.com (imap.hitachi-hta.com [137.168.173.247])
13, 27 -- by sjc-smtp01.hal.hitachi.com (HALSMTP) with SMTP id 01.16.04015.25FEF1B4; Wed, 9 Dec 2009 10:41:22 -0800 (PST)
13, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
13, 27 -- Content-class: urn:content-classes:message
13, 27 -- MIME-Version: 1.0
13, 27 -- Content-Type: text/plain;
13, 27 -- charset="us-ascii"
13, 27 -- Subject: Re: Albert Crewe
13, 27 -- Date: Wed, 9 Dec 2009 12:41:21 -0600
13, 27 -- Message-ID: {A7FCF7B158674D4E83DA2203C3B0ABEA0B39FD65-at-exchange.hitachi-hta.com}
13, 27 -- X-MS-Has-Attach:
13, 27 -- X-MS-TNEF-Correlator:
13, 27 -- Thread-Topic: Re: Albert Crewe
13, 27 -- Thread-Index: Acp4/zOVhzSCr1wwSx6KdVEPBPBMqQ==
13, 27 -- From: "Gschwend, Jeff" {Jeff.Gschwend-at-hitachi-hta.com}
13, 27 -- To: {Microscopy-at-microscopy.com}
13, 27 -- X-Brightmail-Tracker: AAAAAA==
13, 27 -- Content-Transfer-Encoding: 8bit
13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9IfP0P015508
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Wed, 9 Dec 2009 13:39:54 -0600
Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vladimir,

In case the BSE coefficient dependence on Z is establish by fitting a curve plotting BSE coeff. versus Z (atomic number). Then using atomic weight to calculate BSE should not obey the established relationship.

If one extracts the relationship of atomic weight versus BSE coeff. then the relationship is different and then one needs to apply what you suggest.

Krassimir.
============================



On Dec 9, 2009, at 10:31 AM, DusevichV-at-umkc.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Krassimir,
}
} Average atomic number for BSE contrast should be calculated for
} weight (not atomic) fractions, so
} Z=w(1)*z1+w(2)*z2+...
}
} Regards
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 371 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} } -----Original Message-----
} } From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} } Sent: Wednesday, December 09, 2009 11:22 AM
} } To: Dusevich, Vladimir
} } Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number
} }
} }
} }
} }
} }
} -----------------------------------------------------------------------
} } -----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------
} } -----
} }
} } Gary,
} }
} } Simply use the chemical formula of the compound. Add all the Z numbers
} } and divide by the total number of atoms in the formula. E.g. SiO2 = 14
} } + 8 + 8 =30 /3 = 10.
} } To large extent the actual composition and minor components are
} } practically irrelevant since minor differences in average Z cannot be
} } imaged easy by BSE. The contrast differences between the two phases
} you
} } need to image need to be above 10% in order to be imaged successfully.
} } Contrast differences above 1% but below 5% are very difficult to image
} } and require significant efforts.
} }
} } Monte-Carlo simulations would give adequate and precise answer but in
} } most cases it is not worth the time to do the calculations.
} }
} } For simple estimate of contrast you can use the formula:
} } BSE coeff = -0.0254 +0.016Z - 1.86x10-4Z^2 + 8.3x10-7Z^3
} } Contrast = (BSEcoeff 1 - BSE coeff 2)/ BSE coeff 2
} }
} } Regards,
} }
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } phone 951 827 2998
} } fax 951 827 2489
} } bozhilov-at-ucr.edu
} }
} }
} } On Dec 9, 2009, at 6:27 AM, gary.nichols-at-pfizer.com wrote:
} }
} } }
} } }
} } }
} } }
} ---------------------------------------------------------------------
} } -------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} ---------------------------------------------------------------------
} } -------
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
} } } using the WWW based Form at
} } } http://microscopy.com/MicroscopyListserver/MLFormMail.html
} } }
} ---------------------------------------------------------------------
} } ------
} } } Remember this posting is most likely not from a Subscriber, so when
} } replying
} } } please copy both gary.nichols-at-pfizer.com as well as the
} } } MIcroscopy Listserver
} } }
} ---------------------------------------------------------------------
} } ------
} } }
} } } Email: gary.nichols-at-pfizer.com
} } } Name: Gary Nichols
} } }
} } } Title-Subject: [Filtered] Calculation of average atomic number
} } }
} } } Question: Dear Listers,
} } }
} } } When observing backscattered electron images, the relative
} brightness
} } } of objects, such as particles, is (we are lead to believe) a
} function
} } } of the average atomic number(av.Z), so that bright objects have a
} } } high av.Z relative to darker ones having a lower av.Z. This is
} taken
} } } as read by all users of BS detectors and is a well known phenomenon
} } } that is described in books and papers. What I would like to know is
} } } how to calculate the av.Z for a material (e.g., a mineral, an alloy
} } } or an organic compound). I don't like to assume that specimens I am
} } } viewing have a high or low av.Z just by using the relative
} brightness
} } } between objects.
} } }
} } } Thank you.
} } } Gary
} } }
} } }
} } }
} } } Login Host: 168.224.1.14
} } }
} ---------------------------------------------------------------------
} } ------
} } }
} } } ==============================Original
} } Headers==============================
} } } 9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:19:26 2009
} } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP
} } id nB9EJPk6001628
} } } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009
} 08:19:26 -
} } 0600
} } } 9, 11 -- Mime-Version: 1.0
} } } 9, 11 -- Message-Id: {p06240800c745625c513b-at-[206.69.208.22]}
} } } 9, 11 -- Date: Wed, 9 Dec 2009 08:19:24 -0600
} } } 9, 11 -- To: microscopy-at-microscopy.com
} } } 9, 11 -- From: gary.nichols-at-pfizer.com (by way of
} } MicroscopyListserver)
} } } 9, 11 -- Subject: viaWWW: Calculation of average atomic number
} } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ;
} } format="flowed"
} } } ==============================End of -
} } Headers==============================
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 12, 22 -- From bozhilov-at-ucr.edu Wed Dec 9 11:21:04 2009
} } 12, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
} } [138.23.226.212])
} } 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP
} } id nB9HL4Fu016937
} } 12, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009
} 11:21:04 -
} } 0600
} } 12, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
} } 12, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
} } 12, 22 -- with ESMTP id KDB94387;
} } 12, 22 -- Wed, 9 Dec 2009 09:20:59 -0800 (PST)
} } 12, 22 -- Subject: Re: [Microscopy] viaWWW: Calculation of average
} } atomic number
} } 12, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
} } 12, 22 -- Content-Type: text/plain; charset=windows-1252
} } 12, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
} } 12, 22 -- In-Reply-To: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
} } 12, 22 -- Date: Wed, 9 Dec 2009 09:21:01 -0800
} } 12, 22 -- Cc: microscopy-at-microscopy.com
} } 12, 22 -- Message-Id: {36B6A389-AEFE-4926-A110-8BEA86045199-at-ucr.edu}
} } 12, 22 -- References: {200912091427.nB9ER3m0021888-at-ns.microscopy.com}
} } 12, 22 -- To: gary.nichols-at-pfizer.com
} } 12, 22 -- X-Mailer: Apple Mail (2.1077)
} } 12, 22 -- X-Junkmail-Status: score=0/50, host=
} } 12, 22 -- Content-Transfer-Encoding: 8bit
} } 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} } ns.microscopy.com id nB9HL4Fu016937
} } ==============================End of -
} } Headers==============================
}
}
}
} ==============================Original Headers==============================
} 8, 25 -- From DusevichV-at-umkc.edu Wed Dec 9 12:24:46 2009
} 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp3.exchange.umkc.edu [134.193.143.167])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9IOkia000597
} 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 12:24:46 -0600
} 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 8, 25 -- Wed, 9 Dec 2009 12:24:45 -0600
} 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 8, 25 -- Content-class: urn:content-classes:message
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- Content-Type: text/plain;
} 8, 25 -- charset="us-ascii"
} 8, 25 -- Subject: RE: [Microscopy] Re: viaWWW: Calculation of average atomic number
} 8, 25 -- Date: Wed, 9 Dec 2009 12:24:46 -0600
} 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB30935FF5A-at-KC-MSX1.kc.umkc.edu}
} 8, 25 -- In-Reply-To: {200912091721.nB9HLalR017755-at-ns.microscopy.com}
} 8, 25 -- X-MS-Has-Attach:
} 8, 25 -- X-MS-TNEF-Correlator:
} 8, 25 -- thread-topic: [Microscopy] Re: viaWWW: Calculation of average atomic number
} 8, 25 -- thread-index: Acp49BD9XBWxYGohQlmSD3Yl/oDaJgACF9bg
} 8, 25 -- References: {200912091721.nB9HLalR017755-at-ns.microscopy.com}
} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} 8, 25 -- To: {Microscopy-at-microscopy.com}
} 8, 25 -- X-OriginalArrivalTime: 09 Dec 2009 18:24:45.0452 (UTC) FILETIME=[E2230CC0:01CA78FC]
} 8, 25 -- Content-Transfer-Encoding: 8bit
} 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9IOkia000597
} ==============================End of - Headers==============================



==============================Original Headers==============================
10, 22 -- From bozhilov-at-ucr.edu Wed Dec 9 13:39:54 2009
10, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9JdqY6032219
10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 13:39:53 -0600
10, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
10, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
10, 22 -- with ESMTP id KDD51305;
10, 22 -- Wed, 9 Dec 2009 11:39:50 -0800 (PST)
10, 22 -- Subject: Re: [Microscopy] viaWWW: Calculation of average atomic number
10, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
10, 22 -- Content-Type: text/plain; charset=us-ascii
10, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
10, 22 -- In-Reply-To: {200912091831.nB9IVEn3010246-at-ns.microscopy.com}
10, 22 -- Date: Wed, 9 Dec 2009 11:39:49 -0800
10, 22 -- Cc: microscopy-at-microscopy.com
10, 22 -- Message-Id: {D0EAC6F2-137E-428C-AFF5-C3BE4C9C0289-at-ucr.edu}
10, 22 -- References: {200912091831.nB9IVEn3010246-at-ns.microscopy.com}
10, 22 -- To: DusevichV-at-umkc.edu
10, 22 -- X-Mailer: Apple Mail (2.1077)
10, 22 -- X-Junkmail-Status: score=0/50, host=
10, 22 -- Content-Transfer-Encoding: 8bit
10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nB9JdqY6032219
==============================End of - Headers==============================




From: beth-at-plantbio.uga.edu
Date: Wed, 9 Dec 2009 16:27:40 -0600
Subject: [Microscopy] grid glue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
Can you use double-sticky tape to make grid glue or is there an EM
voodoo issue with that kind of tape?

best,
Beth


**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
Miller Plant Sciences Bldg
120 Carlton Street
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com






==============================Original Headers==============================
14, 19 -- From beth-at-plantbio.uga.edu Wed Dec 9 16:27:40 2009
14, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2])
14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9MReNY019172
14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 16:27:40 -0600
14, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
14, 19 -- (authenticated user beth-at-plantbio.uga.edu)
14, 19 -- by dogwood.plantbio.uga.edu
14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
14, 19 -- for microscopy-at-microscopy.com;
14, 19 -- Wed, 9 Dec 2009 17:27:38 -0500
14, 19 -- Message-Id: {2CE9F815-FD8D-40C3-ABA3-C5E89CA1F68D-at-plantbio.uga.edu}
14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu}
14, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com}
14, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
14, 19 -- Content-Transfer-Encoding: 7bit
14, 19 -- Mime-Version: 1.0 (Apple Message framework v936)
14, 19 -- Subject: grid glue
14, 19 -- Date: Wed, 9 Dec 2009 17:27:38 -0500
14, 19 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================




From: waheeschen-at-dow.com
Date: Wed, 9 Dec 2009 18:41:44 -0600
Subject: [Microscopy] viaWWW: Looking for Confocal microscope with 380nm excitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Beth,
That's what I use! Work's great!
Jo Dee


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core





Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish-at-gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Wednesday, December 09, 2009 2:34 PM
To: jfish-at-gladstone.ucsf.edu

Hi all,
Can you use double-sticky tape to make grid glue or is there an EM voodoo
issue with that kind of tape?

best,
Beth


**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
Miller Plant Sciences Bldg
120 Carlton Street
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

***************************************************************************
The Friends of the Marine Institute - Join Today!
www.friendsofugami.com






==============================Original Headers==============================
14, 19 -- From beth-at-plantbio.uga.edu Wed Dec 9 16:27:40 2009 14, 19 --
Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu
[128.192.26.2])
14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nB9MReNY019172
14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 16:27:40
-0600
14, 19 -- Received: from [128.192.26.46] ([128.192.26.46])
14, 19 -- (authenticated user beth-at-plantbio.uga.edu)
14, 19 -- by dogwood.plantbio.uga.edu
14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits))
14, 19 -- for microscopy-at-microscopy.com;
14, 19 -- Wed, 9 Dec 2009 17:27:38 -0500
14, 19 -- Message-Id:
{2CE9F815-FD8D-40C3-ABA3-C5E89CA1F68D-at-plantbio.uga.edu}
14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 14, 19 -- To:
microscopy microscopy {microscopy-at-microscopy.com} 14, 19 -- Content-Type:
text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 19 --
Content-Transfer-Encoding: 7bit 14, 19 -- Mime-Version: 1.0 (Apple Message
framework v936) 14, 19 -- Subject: grid glue 14, 19 -- Date: Wed, 9 Dec 2009
17:27:38 -0500 14, 19 -- X-Mailer: Apple Mail (2.936)
==============================End of - Headers==============================


==============================Original Headers==============================
23, 28 -- From jfish-at-gladstone.ucsf.edu Wed Dec 9 16:47:54 2009
23, 28 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31])
23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9Mlst0001653
23, 28 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 16:47:54 -0600
23, 28 -- X-IronPort-AV: E=Sophos;i="4.47,370,1257148800";
23, 28 -- d="scan'208";a="2824219"
23, 28 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25])
23, 28 -- by gmail2.ucsf.edu with ESMTP; 09 Dec 2009 14:47:53 -0800
23, 28 -- Received: from [169.230.76.4] (HELO JFISH)
23, 28 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 5.2.16)
23, 28 -- with ESMTP id 568539762; Wed, 09 Dec 2009 14:47:29 -0800
23, 28 -- Reply-To: {jfish-at-gladstone.ucsf.edu}
23, 28 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu}
23, 28 -- To: {microscopy-at-microscopy.com}
23, 28 -- Cc: {beth-at-plantbio.uga.edu}
23, 28 -- References: {200912092233.nB9MXwnI027687-at-ns.microscopy.com}
23, 28 -- Subject: RE: [Microscopy] grid glue
23, 28 -- Date: Wed, 9 Dec 2009 14:47:50 -0800
23, 28 -- Organization: J. David Gladstone Institutes
23, 28 -- Message-ID: {D348E2E9064A41E5BAA9BF5B4557D66B-at-JFISH}
23, 28 -- MIME-Version: 1.0
23, 28 -- Content-Type: text/plain;
23, 28 -- charset="us-ascii"
23, 28 -- Content-Transfer-Encoding: 7bit
23, 28 -- X-Mailer: Microsoft Office Outlook 11
23, 28 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.5579
23, 28 -- In-Reply-To: {200912092233.nB9MXwnI027687-at-ns.microscopy.com}
23, 28 -- Thread-Index: Acp5H6TXmx5yCgCSSqKNJckp3gXxTgAAclAg
==============================End of - Headers==============================

From andro99-at-ms66.hinet.net Wed Dec 9 18:18:44 2009
Return-Path: {andro99-at-ms66.hinet.net}
Received: from pool2-69.metrotel.net.co (adsl-pool2-69.metrotel.net.co [190.182.59.69] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA0IgQL018106;
Wed, 9 Dec 2009 18:18:43 -0600
Received: from 190.182.59.69 by ms66a.hinet.net; Wed, 9 Dec 2009 18:18:41 -0600

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both waheeschen-at-dow.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: waheeschen-at-dow.com
Name: Bill Heeschen

Organization: Dow Chemical

Title-Subject: [Filtered] Looking for Confocal microscope with 380nm excitation

Question: Greetings Microscopists:
We are looking for time on a confocal microscope with excitation
somewhere near 380 nm. We are trying to observe a fairly dilute
fluorophore that has a peak absorption at 380nm and emission at about
550nm. We believe they exist in sparse domains that are on the order
of microns in size. If your facility has a 'scope that might be able
to confirm or refute this hypothesis, we would like to set up a
project. Please contact me directly at the e-mail address below.
Being closer to Michigan is better, but not a requirement.

Thanks and Best Regards,

Bill
William A. Heeschen, Ph.D.
Microscopy, Digital Imaging
The Dow Chemical Company
Midland, MI 48667
waheeschen-at-dow.com


Login Host: 216.99.65.63
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 18:41:43 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA0ffdg018671
9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 18:41:42 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240801c745efaa7017-at-[206.69.208.22]}
9, 11 -- Date: Wed, 9 Dec 2009 18:22:35 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: waheeschen-at-dow.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Looking for Confocal microscope with 380nm excitation
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: justinmccoy628-at-gmail.com
Date: Wed, 9 Dec 2009 18:41:44 -0600
Subject: [Microscopy] viaWWW: LM: Dallas Area Vendor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both justinmccoy628-at-gmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: justinmccoy628-at-gmail.com
Name: Justin

Title-Subject: [Filtered] Dallas Area Vendor

Question: Hi,

I am looking for a vendor in the DFW metroplex that can images 15-20
glass slides at 2000x. Any thoughts on where to look for a vendor
that can do this?

Thanks,
JM

Login Host: 70.141.148.162
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 18:41:44 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA0ffdi018671
7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 18:41:44 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c745efdc7bf7-at-[206.69.208.22]}
7, 11 -- Date: Wed, 9 Dec 2009 18:23:24 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: justinmccoy628-at-gmail.com (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: LM: Dallas Area Vendor
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cruzetti-at-hotmail.com
Date: Thu, 10 Dec 2009 19:58:14 -0600
Subject: [Microscopy] viaWWW: Printing digital EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mjamison-at-caissonlabs.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mjamison-at-caissonlabs.com
Name: michelle jamsion

Title-Subject: [Filtered] PEG (Carbowax) sectioning

Question: Hi all
I have been doing thin sections in carbowax and as yet have not
managed a nice flat, consistant mount. The sections always seem to
roll up, then when put onto the slide are mostly twisted and folded.
By the time the samples are infiltrated by the PEG they are quite
hard (ovules of grain crops), possibly that is the reason. If you
have any experience and suggestions with the above, please let me
know.

Thanks very much
Michelle

Login Host: 129.123.46.133
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 18:41:45 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA0ffdk018671
6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 18:41:45 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240803c745f005859b-at-[206.69.208.22]}
6, 11 -- Date: Wed, 9 Dec 2009 18:23:48 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: mjamison-at-caissonlabs.com (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: PEG (Carbowax) sectioning
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From p7505-at-ms2.hinet.net Wed Dec 9 19:48:10 2009
Return-Path: {p7505-at-ms2.hinet.net}
Received: from 200-50-110-50.static.tie.cl (200-50-110-50.static.tie.cl [200.50.110.50])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA1m5MS031458;
Wed, 9 Dec 2009 19:48:07 -0600
Received: from [134.29.12.13] (account a240man-at-adamjeeinsurance.com HELO yqyqqmdsxvmszt.pfqowyiw.net)
by 200-50-110-50.static.tie.cl (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 699431841 for microscopy-at-microscopy.com; Wed, 9 Dec 2009 21:48:06 -0400

Hi Gary,

While the backscatter coefficient does indeed change with the average Z
of the sample you should also keep in mind that channeling effects can
affect the brightness of the BSE image. This primarily occur in samples
that have relatively pristine crystal lattices near the surface. Once
you get an amorphous or damaged layer, the channeling effect will
decrease.

This effect is very evident in ion beam images of polycrystalline
samples. The channeling contrast disappears with higher doses due to
amorphization of the sample surface.

Cheers,
Henk


gary.nichols-at-pfizer.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both gary.nichols-at-pfizer.com as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: gary.nichols-at-pfizer.com
} Name: Gary Nichols
}
} Title-Subject: [Filtered] Calculation of average atomic number
}
} Question: Dear Listers,
}
} When observing backscattered electron images, the relative brightness
} of objects, such as particles, is (we are lead to believe) a function
} of the average atomic number(av.Z), so that bright objects have a
} high av.Z relative to darker ones having a lower av.Z. This is taken
} as read by all users of BS detectors and is a well known phenomenon
} that is described in books and papers. What I would like to know is
} how to calculate the av.Z for a material (e.g., a mineral, an alloy
} or an organic compound). I don't like to assume that specimens I am
} viewing have a high or low av.Z just by using the relative brightness
} between objects.
}
} Thank you.
} Gary
}
}
}
} Login Host: 168.224.1.14
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 11 -- From zaluzec-at-microscopy.com Wed Dec 9 08:19:26 2009
} 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nB9EJPk6001628
} 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 08:19:26 -0600
} 9, 11 -- Mime-Version: 1.0
} 9, 11 -- Message-Id: {p06240800c745625c513b-at-[206.69.208.22]}
} 9, 11 -- Date: Wed, 9 Dec 2009 08:19:24 -0600
} 9, 11 -- To: microscopy-at-microscopy.com
} 9, 11 -- From: gary.nichols-at-pfizer.com (by way of MicroscopyListserver)
} 9, 11 -- Subject: viaWWW: Calculation of average atomic number
} 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
8, 26 -- From colijn.1-at-osu.edu Wed Dec 9 20:50:03 2009
8, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA2o3h6000672
8, 26 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2009 20:50:03 -0600
8, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
8, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 26 -- id {01NH2GHZK1GW96V72P-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
8, 26 -- Wed, 09 Dec 2009 21:50:03 -0500 (EST)
8, 26 -- Received: from [192.168.1.102]
8, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
8, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
8, 26 -- with ESMTPA id {01NH2GHZ2FM896UIAF-at-ecr6.ohio-state.edu} for
8, 26 -- microscopy-at-microscopy.com; Wed, 09 Dec 2009 21:50:02 -0500 (EST)
8, 26 -- Date: Wed, 09 Dec 2009 21:50:02 -0500
8, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
8, 26 -- Subject: Re: [Microscopy] viaWWW: Calculation of average atomic number
8, 26 -- In-reply-to: {200912091421.nB9ELFOQ006125-at-ns.microscopy.com}
8, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
8, 26 -- Cc: microscopy-at-microscopy.com
8, 26 -- Message-id: {4B2061DA.5020204-at-osu.edu}
8, 26 -- MIME-version: 1.0
8, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
8, 26 -- Content-transfer-encoding: 7bit
8, 26 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
8, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
8, 26 -- References: {200912091421.nB9ELFOQ006125-at-ns.microscopy.com}
==============================End of - Headers==============================

From 3dpbqc5581-at-deer-group.com.cn Thu Dec 10 02:03:45 2009
Return-Path: {3dpbqc5581-at-deer-group.com.cn}
Received: from 121.246.243.28.static-chennai.vsnl.net.in (121.246.243.28.static-chennai.vsnl.net.in [121.246.243.28] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBA83he0023411;
Thu, 10 Dec 2009 02:03:44 -0600
Received: from [110.152.158.73] (account t.k-at-herb.ocn.ne.jp HELO nistvyd.roxipzrg.info)
by 121.246.243.28.static-chennai.vsnl.net.in (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 116745710 for microscopy-at-microscopy.com; Thu, 10 Dec 2009 13:33:43 +0530


Good morning everyone,
I'm lookingh for a Zeiss rep in the Cleveland area. I (and my boss) are
interested in newest version of imaging and measurement software. We would
like some literate and a demo if possible. Please feel free to contact me
directly.

Thanks in advance..........

Frank Karl
Microscopist
Lincoln Electric
Cleveland Ohio
216-383-4649

--
*************************************************************
Note:
The information contained in this message may be
privileged and confidential and protected from disclosure. If
the reader of this message is not the intended recipient, or
an employee or agent responsible for delivering this message
to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication
is strictly prohibited. If you have received this
communication in error, please notify us immediately by
replying to the message and deleting it from your computer.
Thank you,
The Lincoln Electric Company
**************************************************************


==============================Original Headers==============================
6, 21 -- From frank_karl-at-lincolnelectric.com Thu Dec 10 05:52:35 2009
6, 21 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBABqZ4b008539
6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2009 05:52:35 -0600
6, 21 -- Subject: looking for area Zeiss rep
6, 21 -- To: Microscopy-at-microscopy.com
6, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005
6, 21 -- Message-ID: {OF773BE072.4AE20171-ON85257688.0040BEF5-85257688.004139F6-at-lincolnelectric.com}
6, 21 -- Date: Thu, 10 Dec 2009 06:52:33 -0500
6, 21 -- From: Frank_Karl-at-lincolnelectric.com
6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 12/10/2009 06:52:33 AM,
6, 21 -- CD-MIME complete at 12/10/2009 06:52:33 AM,
6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 12/10/2009 06:52:33 AM,
6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February
6, 21 -- 07, 2008) at 12/10/2009 06:52:33 AM,
6, 21 -- Serialize complete at 12/10/2009 06:52:33 AM
6, 21 -- MIME-Version: 1.0
6, 21 -- Content-Type: text/plain;
6, 21 -- charset="US-ASCII"
==============================End of - Headers==============================

From 12446xianyundagxmj-at-public.qd.sd.cn Thu Dec 10 08:43:00 2009
Return-Path: {12446xianyundagxmj-at-public.qd.sd.cn}
Received: from [190.209.102.154] ([190.209.102.154])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBAEgxO4030279;
Thu, 10 Dec 2009 08:43:00 -0600
Received: from [94.154.47.129] (helo=likromd.eugdhxeoyvd.org)
by with esmtpa (Exim 4.69)
(envelope-from )
id 1MMQEH-1335is-WX
for microscopy-at-microscopy.com; Thu, 10 Dec 2009 11:42:58 -0300

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cruzetti-at-hotmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: cruzetti-at-hotmail.com
Name: Jeffrey Crews

Organization: Pathology Associates North Carolina

Title-Subject: [Filtered] Printing digital EM

Question: Hello-

My lab is looking into scanning our 4489 negatives and printing them
as digital prints, rather than continuing to use traditional photo
paper and chemistry.

} From searching the archives it looks like the Epson 700 or 750 are
} good choices for the scanning end, but I am still at a loss for a
} good B&W-only photo printer.

We'd need to produce prints that are as permanent and high-res as
traditional prints, if that's possible. We're making 6000+ prints a
year.

Any insights or brand names, etc would be highly appreciated. We're
not pulling the trigger on this yet, but I'd definitely like to start
pulling together some info. Many thanks.

JC

Login Host: 207.247.42.130
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Dec 10 19:58:14 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBB1wD0Z016228
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2009 19:58:14 -0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c747579eceef-at-[206.69.208.22]}
11, 11 -- Date: Thu, 10 Dec 2009 19:58:12 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: cruzetti-at-hotmail.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Printing digital EM
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bozhilov-at-ucr.edu
Date: Fri, 11 Dec 2009 14:12:46 -0600
Subject: [Microscopy] Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kmoulton-at-usm.maine.edu as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: kmoulton-at-usm.maine.edu
Name: Karen Moulton

Organization: University of Southern Maine

Title-Subject: [Filtered] Heliobacterium chlorum

Question: Does anyone have a micrograph they are willing to share, of
Heliobacterium chlorum bacteria or can someone direct me to a place
on the web that will? I can find images of these bacteria (or those
that are related) but none of the images seem to have scale bars,
which is something that is required for a seventh grade project I am
evaluating.

Thank you, Karen

Login Host: 130.111.126.59
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Thu Dec 10 19:58:45 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBB1wiBb016652
7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2009 19:58:45 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c74757bbd5c0-at-[206.69.208.22]}
7, 11 -- Date: Thu, 10 Dec 2009 19:58:44 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: kmoulton-at-usm.maine.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Heliobacterium chlorum
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From aaax.bbs-at-adamjeeinsurance.com Thu Dec 10 23:12:19 2009
Return-Path: {aaax.bbs-at-adamjeeinsurance.com}
Received: from solo20.big.net.ua (solo20.big.net.ua [91.197.184.10])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBB5CIAk017845;
Thu, 10 Dec 2009 23:12:19 -0600
Received: from [33.142.87.50] (account a1211555dd-at-ms2.hinet.net HELO rlslsvbzxhxk.qrrowxox.su)
by solo20.big.net.ua (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 606041464 for microscopy-at-microscopy.com; Fri, 11 Dec 2009 07:13:28 +0200

Hello Lister,
Anyone out there with the capability to re-tip a Denka Lab6? The
crystal is in perfect shape, just the wire that bonds to the filament
post were broken. Seems a shame to throw away a $800
filament.....Thanks in advance

Gary Easton
Scanners Corporation
SEM Service
410-857-7633
--


==============================Original Headers==============================
3, 20 -- From garyeaston-at-scannerscorp.com Fri Dec 11 08:57:24 2009
3, 20 -- Received: from omr12.networksolutionsemail.com (omr12.networksolutionsemail.com [205.178.146.62])
3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBEvNVl004874
3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 08:57:24 -0600
3, 20 -- Received: from cm-omr5 (mail.networksolutionsemail.com [205.178.146.50])
3, 20 -- by omr12.networksolutionsemail.com (8.13.6/8.13.6) with ESMTP id nBBEvLbP016385
3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 09:57:23 -0500
3, 20 -- Received: from [72.81.190.77] ([72.81.190.77:58805] helo=[192.168.1.3])
3, 20 -- by cm-omr5 (envelope-from {garyeaston-at-scannerscorp.com} )
3, 20 -- (ecelerity 2.2.2.41 r(31179/31189)) with ESMTP
3, 20 -- id 03/AB-29347-0DD522B4; Fri, 11 Dec 2009 09:57:21 -0500
3, 20 -- Message-ID: {4B225DB7.4050804-at-scannerscorp.com}
3, 20 -- Date: Fri, 11 Dec 2009 09:56:55 -0500
3, 20 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com}
3, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.1.5) Gecko/20091204 Thunderbird/3.0
3, 20 -- MIME-Version: 1.0
3, 20 -- To: Microscopy Society of America {microscopy-at-microscopy.com}
3, 20 -- Subject: Denka Lab6 Tip
3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From aanheinenbloeme-at-adamjeeinsurance.com Fri Dec 11 10:34:01 2009
Return-Path: {aanheinenbloeme-at-adamjeeinsurance.com}
Received: from [113.254.9.172] ([113.254.9.172])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBGXx07021980;
Fri, 11 Dec 2009 10:34:00 -0600
Received: from [158.172.51.95] (account aanheinenbloeme-at-adamjeeinsurance.com HELO tbudxbvmwuhqvi.bbzptbf.su)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 399259092 for microscopy-at-microscopy.com; Sat, 12 Dec 2009 00:33:59 +0800

It must be a little slow around here. I decided I should go back and
revisit this issue myself. Who am I to argue with Goldstein et al?
However, this did play a significant part in my doctoral research many
moons ago.

Bottom line - Goldstein and company are right. Use the mass fractions of
the element in the compound. Details follow.

I pulled out several compounds, standards and otherwise, that we had
around the lab. I included Al, Mg, CaCO3, SiO2, MgO, SiC, and C. All but
SiC were mounted in epoxy resin and coated with carbon.

I imaged the samples in variable pressure mode using 20 kV. A Tetra BSE
detector (Oxford Instruments) was used for imaging. The brightness and
contrast were set at the outset of the experiment and left set for the
duration (~35 minutes). Images were recorded at either 1000 or 2000x
with an image of CaCO3 collected at both ends.

Multiple, uniform areas of each image were selected for gray scale
analysis. That was done for both the phase of interest and the adjoining
epoxy. The values were tabulated in a spreadsheet to plot the
correlation between measured brightness. The spreadsheet and images are
available from our website
(ftp://www.marl.iastate.edu/General/BSE_ANF/).

According to Krassimir, CaCO3, SiC, SiO2, and MgO should all have an
average atomic number of 10 by simply averaging the atomic numbers
(weighting by mole fraction). However, if the calculation is done by
mass fraction, the average atomic numbers should be 12.6, 11.6, 10.8 and
10.4.

The latter is a much better fit to the measured brightness, i.e., the
brightness is related to the mass-weighted average of atomic numbers.
There is some deviation from a straight line, but the mass-weighted
average better represents the clear difference in brightness measured
for CaCO3, SiC, SiO2, and MgO.

There are a number of factors I might investigate to improve the
agreement. (For starters, I could repolish and recoat the standards to
be able to operate at high vacuum. In VP mode, the brightness of the
adjoining phase pulls at true brightness of the phase.) I might also
extend the measurement to a broader range of atomic numbers. But for
this discussion, I trust that the point is made.

Warren

-----Original Message-----
X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
Sent: Wednesday, December 09, 2009 1:41 PM
To: wesaia-at-iastate.edu

Warren,

You have done a nice experiment to bring up your point.

I agree that my original statement about simply comparing average atomic number values based on atomic concentrations was incorrect and it was not what I intended to state anyways. It was an erroneous statement typed in a hurry.

Still the mass fraction compound approach does not provide adequate answer either.

If you look at your data the observed brightness between compounds such as SiO2, MgO and CaCO3 more closely matches the relationship between the ratio of the atomic weight of the main heavy elements in the compound in this case Si, Mg and Ca rather than the average compound mass fraction.

For example the atomic weight Mg/Si ratio is 0.84 the observed MgO/SiO2 intensity ratio is 0.8, whereas your calculated mass fraction ratio is 0.96
For calcite-quartz the situation is similar. Si/Ca ratio is 0.7, the observed intensity ratio is 0.70 but the mass fraction ratio is 0.85.

So average compound mass fraction does not seem to predict correctly the observed BSE intensity.


Krassimir.
============================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

phone 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu


On Dec 11, 2009, at 10:24 AM, wesaia-at-iastate.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} It must be a little slow around here. I decided I should go back and
} revisit this issue myself. Who am I to argue with Goldstein et al?
} However, this did play a significant part in my doctoral research many
} moons ago.
}
} Bottom line - Goldstein and company are right. Use the mass fractions of
} the element in the compound. Details follow.
}
} I pulled out several compounds, standards and otherwise, that we had
} around the lab. I included Al, Mg, CaCO3, SiO2, MgO, SiC, and C. All but
} SiC were mounted in epoxy resin and coated with carbon.
}
} I imaged the samples in variable pressure mode using 20 kV. A Tetra BSE
} detector (Oxford Instruments) was used for imaging. The brightness and
} contrast were set at the outset of the experiment and left set for the
} duration (~35 minutes). Images were recorded at either 1000 or 2000x
} with an image of CaCO3 collected at both ends.
}
} Multiple, uniform areas of each image were selected for gray scale
} analysis. That was done for both the phase of interest and the adjoining
} epoxy. The values were tabulated in a spreadsheet to plot the
} correlation between measured brightness. The spreadsheet and images are
} available from our website
} (ftp://www.marl.iastate.edu/General/BSE_ANF/).
}
} According to Krassimir, CaCO3, SiC, SiO2, and MgO should all have an
} average atomic number of 10 by simply averaging the atomic numbers
} (weighting by mole fraction). However, if the calculation is done by
} mass fraction, the average atomic numbers should be 12.6, 11.6, 10.8 and
} 10.4.
}
} The latter is a much better fit to the measured brightness, i.e., the
} brightness is related to the mass-weighted average of atomic numbers.
} There is some deviation from a straight line, but the mass-weighted
} average better represents the clear difference in brightness measured
} for CaCO3, SiC, SiO2, and MgO.
}
} There are a number of factors I might investigate to improve the
} agreement. (For starters, I could repolish and recoat the standards to
} be able to operate at high vacuum. In VP mode, the brightness of the
} adjoining phase pulls at true brightness of the phase.) I might also
} extend the measurement to a broader range of atomic numbers. But for
} this discussion, I trust that the point is made.
}
} Warren
}
} -----Original Message-----
} X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} Sent: Wednesday, December 09, 2009 1:41 PM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number
}
} Vladimir,
}
} In case the BSE coefficient dependence on Z is establish by fitting a
} curve plotting BSE coeff. versus Z (atomic number). Then using atomic
} weight to calculate BSE should not obey the established relationship.
}
} If one extracts the relationship of atomic weight versus BSE coeff. then
} the relationship is different and then one needs to apply what you
} suggest.
}
} Krassimir.
} ============================
}
}
} On Dec 9, 2009, at 10:31 AM, DusevichV-at-umkc.edu wrote:
} }
} } Krassimir,
} }
} } Average atomic number for BSE contrast should be calculated for
} } weight (not atomic) fractions, so
} } Z=w(1)*z1+w(2)*z2+...
} }
} } Regards
} }
} } Vladimir
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 371 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } } -----Original Message-----
} } } From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} } } Sent: Wednesday, December 09, 2009 11:22 AM
} } } To: Dusevich, Vladimir
} } } Subject: [Microscopy] Re: viaWWW: Calculation of average atomic
} number
} } }
} } }
} } } Gary,
} } }
} } } Simply use the chemical formula of the compound. Add all the Z
} numbers
} } } and divide by the total number of atoms in the formula. E.g. SiO2 =
} 14
} } } + 8 + 8 =30 /3 = 10.
} } } To large extent the actual composition and minor components are
} } } practically irrelevant since minor differences in average Z cannot be
} } } imaged easy by BSE. The contrast differences between the two phases
} } you
} } } need to image need to be above 10% in order to be imaged
} successfully.
} } } Contrast differences above 1% but below 5% are very difficult to
} image
} } } and require significant efforts.
} } }
} } } Monte-Carlo simulations would give adequate and precise answer but in
} } } most cases it is not worth the time to do the calculations.
} } }
} } } For simple estimate of contrast you can use the formula:
} } } BSE coeff = -0.0254 +0.016Z - 1.86x10-4Z^2 + 8.3x10-7Z^3
} } } Contrast = (BSEcoeff 1 - BSE coeff 2)/ BSE coeff 2
} } }
} } } Regards,
} } }
} } } Krassimir N. Bozhilov
} } } Central Facility for Advanced Microscopy and Microanalysis
} } } University of California
} } } Riverside, CA 92521
} } }
} } } phone 951 827 2998
} } } fax 951 827 2489
} } } bozhilov-at-ucr.edu
} } }
} } }
} } } On Dec 9, 2009, at 6:27 AM, gary.nichols-at-pfizer.com wrote:
} } } }
} } } }
} } ---------------------------------------------------------------------
} } } } Remember this posting is most likely not from a Subscriber, so when
} } } replying
} } } } please copy both gary.nichols-at-pfizer.com as well as the
} } } } MIcroscopy Listserver
} } ---------------------------------------------------------------------
} } } }
} } } } Email: gary.nichols-at-pfizer.com
} } } } Name: Gary Nichols
} } } }
} } } } Title-Subject: [Filtered] Calculation of average atomic number
} } } }
} } } } Question: Dear Listers,
} } } }
} } } } When observing backscattered electron images, the relative
} } brightness
} } } } of objects, such as particles, is (we are lead to believe) a
} } function
} } } } of the average atomic number(av.Z), so that bright objects have a
} } } } high av.Z relative to darker ones having a lower av.Z. This is
} } taken
} } } } as read by all users of BS detectors and is a well known phenomenon
} } } } that is described in books and papers. What I would like to know is
} } } } how to calculate the av.Z for a material (e.g., a mineral, an alloy
} } } } or an organic compound). I don't like to assume that specimens I am
} } } } viewing have a high or low av.Z just by using the relative
} } brightness
} } } } between objects.
} } } }
} } } } Thank you.
} } } } Gary
} } } }
} } } } Login Host: 168.224.1.14
}
}
} ==============================Original Headers==============================
} 17, 37 -- From wesaia-at-iastate.edu Fri Dec 11 12:17:50 2009
} 17, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
} 17, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBIHoI4024880
} 17, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 12:17:50 -0600
} 17, 37 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48])
} 17, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id nBBIHnn1009625;
} 17, 37 -- Fri, 11 Dec 2009 12:17:49 -0600
} 17, 37 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp
} 17, 37 -- id 28ca_7db60aba_e681_11de_be24_001372578af6;
} 17, 37 -- Fri, 11 Dec 2009 12:17:49 -0600
} 17, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
} 17, 37 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id nBBIHmMR008287;
} 17, 37 -- Fri, 11 Dec 2009 12:17:48 -0600
} 17, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 17, 37 -- Fri, 11 Dec 2009 12:17:48 -0600
} 17, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 17, 37 -- Content-class: urn:content-classes:message
} 17, 37 -- MIME-Version: 1.0
} 17, 37 -- Content-Type: text/plain;
} 17, 37 -- charset="us-ascii"
} 17, 37 -- Subject: RE: [Microscopy] Calculation of average atomic number
} 17, 37 -- Date: Fri, 11 Dec 2009 12:19:36 -0600
} 17, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270462F08D-at-maire.eng.iastate.edu}
} 17, 37 -- In-Reply-To: {200912091940.nB9Jekod000754-at-ns.microscopy.com}
} 17, 37 -- X-MS-Has-Attach:
} 17, 37 -- X-MS-TNEF-Correlator:
} 17, 37 -- Thread-Topic: [Microscopy] Calculation of average atomic number
} 17, 37 -- Thread-Index: Acp5B4IADKl+Ix9hTpmPs4JHEJmxewBgUTcA
} 17, 37 -- References: {200912091940.nB9Jekod000754-at-ns.microscopy.com}
} 17, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 17, 37 -- To: {Microscopy-at-microscopy.com}
} 17, 37 -- Cc: {gary.nichols-at-pfizer.com}
} 17, 37 -- X-OriginalArrivalTime: 11 Dec 2009 18:17:48.0401 (UTC) FILETIME=[3E619E10:01CA7A8E]
} 17, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.12.11.180617
} 17, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0'
} 17, 37 -- Content-Transfer-Encoding: 8bit
} 17, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBBIHoI4024880
} ==============================End of - Headers==============================



==============================Original Headers==============================
15, 22 -- From bozhilov-at-ucr.edu Fri Dec 11 14:12:46 2009
15, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212])
15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBKCjx1010291
15, 22 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 14:12:45 -0600
15, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
15, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
15, 22 -- with ESMTP id KDX31984;
15, 22 -- Fri, 11 Dec 2009 12:12:41 -0800 (PST)
15, 22 -- Subject: Re: [Microscopy] RE: Calculation of average atomic number
15, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
15, 22 -- Content-Type: text/plain; charset=us-ascii
15, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
15, 22 -- In-Reply-To: {200912111824.nBBIOGk6002037-at-ns.microscopy.com}
15, 22 -- Date: Fri, 11 Dec 2009 12:12:40 -0800
15, 22 -- Cc: microscopy-at-microscopy.com
15, 22 -- Message-Id: {5BF5728D-C801-4E95-A6DF-C5F6B3DAB4F6-at-ucr.edu}
15, 22 -- References: {200912111824.nBBIOGk6002037-at-ns.microscopy.com}
15, 22 -- To: wesaia-at-iastate.edu
15, 22 -- X-Mailer: Apple Mail (2.1077)
15, 22 -- X-Junkmail-Status: score=0/50, host=
15, 22 -- Content-Transfer-Encoding: 8bit
15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBBKCjx1010291
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Fri, 11 Dec 2009 14:48:20 -0600
Subject: [Microscopy] Re: viaWWW: PEG (Carbowax) sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I did a project a few years ago sectioning PEG 1600 (I might
have the mwt a bit out but it was not super high mwt PEG). I worked
in a "cool" room, held at 16 C which seemed to help. But it was
certainly difficult to get good sections reliably. As I recall I was
cutting with a double edged razor blade, rather than a paraffin
knife. Sorry I can't be more helpful.

Tobias

}
} Email: mjamison-at-caissonlabs.com
} Name: michelle jamsion
}
} Title-Subject: [Filtered] PEG (Carbowax) sectioning
}
} Question: Hi all
} I have been doing thin sections in carbowax and as yet have not
} managed a nice flat, consistant mount. The sections always seem to
} roll up, then when put onto the slide are mostly twisted and folded.
} By the time the samples are infiltrated by the PEG they are quite
} hard (ovules of grain crops), possibly that is the reason. If you
} have any experience and suggestions with the above, please let me
} know.
}
} Thanks very much
} Michelle
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
4, 21 -- From baskin-at-bio.umass.edu Fri Dec 11 14:48:20 2009
4, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBKmKMJ025673
4, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 14:48:20 -0600
4, 21 -- Received: from [172.30.52.170] (eutopia.bio.umass.edu [128.119.55.30])
4, 21 -- (authenticated bits=0)
4, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id nBBKmGkT012421
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
4, 21 -- Fri, 11 Dec 2009 15:48:18 -0500 (EST)
4, 21 -- Mime-Version: 1.0
4, 21 -- Message-Id: {p06240505c7485f409d53-at-[172.30.52.170]}
4, 21 -- In-Reply-To: {200912100042.nBA0gpeo020887-at-ns.microscopy.com}
4, 21 -- References: {200912100042.nBA0gpeo020887-at-ns.microscopy.com}
4, 21 -- Date: Fri, 11 Dec 2009 15:48:15 -0500
4, 21 -- To: mjamison-at-caissonlabs.com
4, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 21 -- Subject: Re: [Microscopy] viaWWW: PEG (Carbowax) sectioning
4, 21 -- Cc: microscopy-at-microscopy.com
4, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Fri, 11 Dec 2009 15:48:18 -0500 (EST)
4, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================




From: ph2-at-sprynet.com
Date: Fri, 11 Dec 2009 14:54:10 -0600
Subject: [Microscopy] Calculation of average atomic number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI:

See

Donovan, Averaging of Backscatter Intensities in Compounds, J Res Nat Inst
Stand Tech, 107, 547-553, 2002

Abstract:

"Low uncertainty measurements on pure element stable isotope pairs
demonstrate that mass has no influence on the backscattering of electrons at
typical electron microprobe energies. The traditional prediction of average
backscatter intensities in compounds using elemental mass fractions is
improperly grounded in mass and thus has no physical basis. We propose an
alternative model to mass fraction averaging, based of the number of
electrons or protons, termed "electron fraction," which predicts backscatter
yield better than mass fraction averaging."




Tony


......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
www.ph2llc.com

(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.


-----Original Message-----
X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
Sent: Friday, December 11, 2009 3:18 PM
To: ph2-at-sprynet.com

Warren,

You have done a nice experiment to bring up your point.

I agree that my original statement about simply comparing average atomic
number values based on atomic concentrations was incorrect and it was not
what I intended to state anyways. It was an erroneous statement typed in a
hurry.

Still the mass fraction compound approach does not provide adequate answer
either.

If you look at your data the observed brightness between compounds such as
SiO2, MgO and CaCO3 more closely matches the relationship between the ratio
of the atomic weight of the main heavy elements in the compound in this case
Si, Mg and Ca rather than the average compound mass fraction.

For example the atomic weight Mg/Si ratio is 0.84 the observed MgO/SiO2
intensity ratio is 0.8, whereas your calculated mass fraction ratio is 0.96
For calcite-quartz the situation is similar. Si/Ca ratio is 0.7, the
observed intensity ratio is 0.70 but the mass fraction ratio is 0.85.

So average compound mass fraction does not seem to predict correctly the
observed BSE intensity.


Krassimir.
============================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

phone 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu


On Dec 11, 2009, at 10:24 AM, wesaia-at-iastate.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} It must be a little slow around here. I decided I should go back and
} revisit this issue myself. Who am I to argue with Goldstein et al?
} However, this did play a significant part in my doctoral research many
} moons ago.
}
} Bottom line - Goldstein and company are right. Use the mass fractions of
} the element in the compound. Details follow.
}
} I pulled out several compounds, standards and otherwise, that we had
} around the lab. I included Al, Mg, CaCO3, SiO2, MgO, SiC, and C. All but
} SiC were mounted in epoxy resin and coated with carbon.
}
} I imaged the samples in variable pressure mode using 20 kV. A Tetra BSE
} detector (Oxford Instruments) was used for imaging. The brightness and
} contrast were set at the outset of the experiment and left set for the
} duration (~35 minutes). Images were recorded at either 1000 or 2000x
} with an image of CaCO3 collected at both ends.
}
} Multiple, uniform areas of each image were selected for gray scale
} analysis. That was done for both the phase of interest and the adjoining
} epoxy. The values were tabulated in a spreadsheet to plot the
} correlation between measured brightness. The spreadsheet and images are
} available from our website
} (ftp://www.marl.iastate.edu/General/BSE_ANF/).
}
} According to Krassimir, CaCO3, SiC, SiO2, and MgO should all have an
} average atomic number of 10 by simply averaging the atomic numbers
} (weighting by mole fraction). However, if the calculation is done by
} mass fraction, the average atomic numbers should be 12.6, 11.6, 10.8 and
} 10.4.
}
} The latter is a much better fit to the measured brightness, i.e., the
} brightness is related to the mass-weighted average of atomic numbers.
} There is some deviation from a straight line, but the mass-weighted
} average better represents the clear difference in brightness measured
} for CaCO3, SiC, SiO2, and MgO.
}
} There are a number of factors I might investigate to improve the
} agreement. (For starters, I could repolish and recoat the standards to
} be able to operate at high vacuum. In VP mode, the brightness of the
} adjoining phase pulls at true brightness of the phase.) I might also
} extend the measurement to a broader range of atomic numbers. But for
} this discussion, I trust that the point is made.
}
} Warren
}
} -----Original Message-----
} X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} Sent: Wednesday, December 09, 2009 1:41 PM
} To: wesaia-at-iastate.edu
} Subject: [Microscopy] Re: viaWWW: Calculation of average atomic number
}
} Vladimir,
}
} In case the BSE coefficient dependence on Z is establish by fitting a
} curve plotting BSE coeff. versus Z (atomic number). Then using atomic
} weight to calculate BSE should not obey the established relationship.
}
} If one extracts the relationship of atomic weight versus BSE coeff. then
} the relationship is different and then one needs to apply what you
} suggest.
}
} Krassimir.
} ============================
}
}
} On Dec 9, 2009, at 10:31 AM, DusevichV-at-umkc.edu wrote:
} }
} } Krassimir,
} }
} } Average atomic number for BSE contrast should be calculated for
} } weight (not atomic) fractions, so
} } Z=w(1)*z1+w(2)*z2+...
} }
} } Regards
} }
} } Vladimir
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 371 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } } -----Original Message-----
} } } From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} } } Sent: Wednesday, December 09, 2009 11:22 AM
} } } To: Dusevich, Vladimir
} } } Subject: [Microscopy] Re: viaWWW: Calculation of average atomic
} number
} } }
} } }
} } } Gary,
} } }
} } } Simply use the chemical formula of the compound. Add all the Z
} numbers
} } } and divide by the total number of atoms in the formula. E.g. SiO2 =
} 14
} } } + 8 + 8 =30 /3 = 10.
} } } To large extent the actual composition and minor components are
} } } practically irrelevant since minor differences in average Z cannot be
} } } imaged easy by BSE. The contrast differences between the two phases
} } you
} } } need to image need to be above 10% in order to be imaged
} successfully.
} } } Contrast differences above 1% but below 5% are very difficult to
} image
} } } and require significant efforts.
} } }
} } } Monte-Carlo simulations would give adequate and precise answer but in
} } } most cases it is not worth the time to do the calculations.
} } }
} } } For simple estimate of contrast you can use the formula:
} } } BSE coeff = -0.0254 +0.016Z - 1.86x10-4Z^2 + 8.3x10-7Z^3
} } } Contrast = (BSEcoeff 1 - BSE coeff 2)/ BSE coeff 2
} } }
} } } Regards,
} } }
} } } Krassimir N. Bozhilov
} } } Central Facility for Advanced Microscopy and Microanalysis
} } } University of California
} } } Riverside, CA 92521
} } }
} } } phone 951 827 2998
} } } fax 951 827 2489
} } } bozhilov-at-ucr.edu
} } }
} } }
} } } On Dec 9, 2009, at 6:27 AM, gary.nichols-at-pfizer.com wrote:
} } } }
} } } }
} } ---------------------------------------------------------------------
} } } } Remember this posting is most likely not from a Subscriber, so when
} } } replying
} } } } please copy both gary.nichols-at-pfizer.com as well as the
} } } } MIcroscopy Listserver
} } ---------------------------------------------------------------------
} } } }
} } } } Email: gary.nichols-at-pfizer.com
} } } } Name: Gary Nichols
} } } }
} } } } Title-Subject: [Filtered] Calculation of average atomic number
} } } }
} } } } Question: Dear Listers,
} } } }
} } } } When observing backscattered electron images, the relative
} } brightness
} } } } of objects, such as particles, is (we are lead to believe) a
} } function
} } } } of the average atomic number(av.Z), so that bright objects have a
} } } } high av.Z relative to darker ones having a lower av.Z. This is
} } taken
} } } } as read by all users of BS detectors and is a well known phenomenon
} } } } that is described in books and papers. What I would like to know is
} } } } how to calculate the av.Z for a material (e.g., a mineral, an alloy
} } } } or an organic compound). I don't like to assume that specimens I am
} } } } viewing have a high or low av.Z just by using the relative
} } brightness
} } } } between objects.
} } } }
} } } } Thank you.
} } } } Gary
} } } }
} } } } Login Host: 168.224.1.14
}
}
} ==============================Original
Headers==============================
} 17, 37 -- From wesaia-at-iastate.edu Fri Dec 11 12:17:50 2009
} 17, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu
[129.186.140.15])
} 17, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nBBIHoI4024880
} 17, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 12:17:50
-0600
} 17, 37 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu
[129.186.1.48])
} 17, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with
SMTP id nBBIHnn1009625;
} 17, 37 -- Fri, 11 Dec 2009 12:17:49 -0600
} 17, 37 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by
devirus-11.iastate.edu with smtp
} 17, 37 -- id 28ca_7db60aba_e681_11de_be24_001372578af6;
} 17, 37 -- Fri, 11 Dec 2009 12:17:49 -0600
} 17, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu
[129.186.23.85])
} 17, 37 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with
ESMTP id nBBIHmMR008287;
} 17, 37 -- Fri, 11 Dec 2009 12:17:48 -0600
} 17, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by
owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
} 17, 37 -- Fri, 11 Dec 2009 12:17:48 -0600
} 17, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 17, 37 -- Content-class: urn:content-classes:message
} 17, 37 -- MIME-Version: 1.0
} 17, 37 -- Content-Type: text/plain;
} 17, 37 -- charset="us-ascii"
} 17, 37 -- Subject: RE: [Microscopy] Calculation of average atomic number
} 17, 37 -- Date: Fri, 11 Dec 2009 12:19:36 -0600
} 17, 37 -- Message-ID:
{16A330AC32056A40B32842EC4BB8D7270462F08D-at-maire.eng.iastate.edu}
} 17, 37 -- In-Reply-To: {200912091940.nB9Jekod000754-at-ns.microscopy.com}
} 17, 37 -- X-MS-Has-Attach:
} 17, 37 -- X-MS-TNEF-Correlator:
} 17, 37 -- Thread-Topic: [Microscopy] Calculation of average atomic number
} 17, 37 -- Thread-Index: Acp5B4IADKl+Ix9hTpmPs4JHEJmxewBgUTcA
} 17, 37 -- References: {200912091940.nB9Jekod000754-at-ns.microscopy.com}
} 17, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
} 17, 37 -- To: {Microscopy-at-microscopy.com}
} 17, 37 -- Cc: {gary.nichols-at-pfizer.com}
} 17, 37 -- X-OriginalArrivalTime: 11 Dec 2009 18:17:48.0401 (UTC)
FILETIME=[3E619E10:01CA7A8E]
} 17, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912,
Antispam-Data: 2009.12.11.180617
} 17, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%,
Report='BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0,
__BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0,
__CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0,
__FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0,
__MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0,
__TO_MALFORMED_2 0'
} 17, 37 -- Content-Transfer-Encoding: 8bit
} 17, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nBBIHoI4024880
} ==============================End of -
Headers==============================



==============================Original Headers==============================
15, 22 -- From bozhilov-at-ucr.edu Fri Dec 11 14:12:46 2009
15, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu
[138.23.226.212])
15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nBBKCjx1010291
15, 22 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 14:12:45
-0600
15, 22 -- Received: from [138.23.185.168] ([138.23.185.168])
15, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA)
15, 22 -- with ESMTP id KDX31984;
15, 22 -- Fri, 11 Dec 2009 12:12:41 -0800 (PST)
15, 22 -- Subject: Re: [Microscopy] RE: Calculation of average atomic number
15, 22 -- Mime-Version: 1.0 (Apple Message framework v1077)
15, 22 -- Content-Type: text/plain; charset=us-ascii
15, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu}
15, 22 -- In-Reply-To: {200912111824.nBBIOGk6002037-at-ns.microscopy.com}
15, 22 -- Date: Fri, 11 Dec 2009 12:12:40 -0800
15, 22 -- Cc: microscopy-at-microscopy.com
15, 22 -- Message-Id: {5BF5728D-C801-4E95-A6DF-C5F6B3DAB4F6-at-ucr.edu}
15, 22 -- References: {200912111824.nBBIOGk6002037-at-ns.microscopy.com}
15, 22 -- To: wesaia-at-iastate.edu
15, 22 -- X-Mailer: Apple Mail (2.1077)
15, 22 -- X-Junkmail-Status: score=0/50, host=
15, 22 -- Content-Transfer-Encoding: 8bit
15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nBBKCjx1010291
==============================End of - Headers==============================


==============================Original Headers==============================
34, 28 -- From ph2-at-sprynet.com Fri Dec 11 14:54:10 2009
34, 28 -- Received: from elasmtp-scoter.atl.sa.earthlink.net (elasmtp-scoter.atl.sa.earthlink.net [209.86.89.67])
34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBBKsAf6005656
34, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 14:54:10 -0600
34, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
34, 28 -- s=dk20050327; d=sprynet.com;
34, 28 -- b=JXFs5+0kP3NoLFwAO9N9IlKSemjl5X24UvmsWWd7re4CC6w+bdLD5cMd4jzujOWz;
34, 28 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:In-Reply-To:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP;
34, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284)
34, 28 -- by elasmtp-scoter.atl.sa.earthlink.net with esmtpa (Exim 4.67)
34, 28 -- (envelope-from {ph2-at-sprynet.com} )
34, 28 -- id 1NJCV7-0000RX-GF; Fri, 11 Dec 2009 15:54:09 -0500
34, 28 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
34, 28 -- To: {bozhilov-at-ucr.edu}
34, 28 -- Cc: "Microscopy Listserve" {microscopy-at-microscopy.com}
34, 28 -- Subject: RE: [Microscopy] Calculation of average atomic number
34, 28 -- Date: Fri, 11 Dec 2009 15:54:04 -0500
34, 28 -- MIME-Version: 1.0
34, 28 -- Content-Type: text/plain;
34, 28 -- charset="us-ascii"
34, 28 -- Content-Transfer-Encoding: 7bit
34, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
34, 28 -- Thread-Index: Acp6nv9KoNprzJTnTKyBWmKziRTOjQABM+rA
34, 28 -- In-Reply-To: {200912112017.nBBKHhos017234-at-ns.microscopy.com}
34, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350
34, 28 -- Message-ID: {E1NJCV7-0000RX-GF-at-elasmtp-scoter.atl.sa.earthlink.net}
34, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f98852e2de8cee24aefa3e55bdb07d6d20350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
34, 28 -- X-Originating-IP: 75.61.18.94
==============================End of - Headers==============================




From: gw265-at-cam.ac.uk
Date: Sat, 12 Dec 2009 04:12:34 -0600
Subject: [Microscopy] biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At least two points need to be clarified.

First, the intensity scale does not bottom out with an intensity of 0
for ANF = 0. It will all depend on the brightness and contrast settings
on a given day. I only hope that the response is linear over the range.

In practice, I tweak the brightness and gamma to get phases to show up.
Rarely do I try to quantify the signal level. I am satisfied with
qualitatively getting one phase to show up next to another.

Therefore, forget ratioing the intensity levels.

Second, you may give me too much credit for my measurements. My purpose
was to demonstrate the error in what you admit was an incorrect
statement, the formula that led to the same ANF for MgO, SiO2, and
CaCO3. They clearly vary in intensity.

You refer to the mass fraction ratio in your comments. I take that to
mean the atomic number factor determined by mass-fraction weighting of
the atomic numbers. I will freely admit that SiO2 and Al seem to fall
below the line. However the weighted ANF does seem to nicely explain the
intensity trend between MgO and Mg.

I may see if I can make some more measurements next week. But first, I
should probably read Donovan's paper. I also have a few Christmastime
functions to go to over the weekend, so I will have to see how I'm doing
at the beginning of next week.

Till then, cheers.

-----Original Message-----
X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
Sent: Friday, December 11, 2009 2:13 PM
To: wesaia-at-iastate.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dridings-at-carolinas.org as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dridings-at-carolinas.org
Name: Daisy Ridings

Organization: Carolinas HealthCare System

Title-Subject: [Filtered] Open TEM Technician Position

Question: Carolinas Medical Center in Charlotte,
NC is seeking a full-time electron microscopy
technician. The position involves clinical and
research work. Duties include operation and
basic maintenance of the transmission electron
microscope, sample preparation, thick and thin
sectioning, staining, and digital imaging.

Other required skills include the ability to work
independently and interdependently as part of a
high performance team and the ability to
troubleshoot problems with techniques and
equipment. Experience with digital imaging and
Microsoft Office applications is highly desired.
Immunocytochemistry experience is also helpful.

Bachelorís degree required. Masterís degree and
previous TEM experience preferred.

Applicants must apply online at
http://careers.carolinashealthcare.org [Position
# 198505] or
http://careers.carolinashealthcare.org/JobDetail.aspx?Pagecode=1&jobid=498234
(direct link).

Carolinas Medical Center is an Academic Medical
Center Teaching Hospital and the flagship
facility of Carolinas HealthCare System.
Carolinas HealthCare System is an
equal-opportunity not-for-profit, self-supporting
public organization offering a wide variety of
health and human services to residents of both
North and South Carolina. Carolinas HealthCare
System is the largest healthcare system in the
Carolinas, and the third largest public system in
the nation.


Login Host: 66.194.118.10
---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Fri Dec 11 18:12:08 2009
12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBC0C8hi011480
12, 13 -- for {microscopy-at-microscopy.com} ; Fri, 11 Dec 2009 18:12:08 -0600
12, 13 -- Mime-Version: 1.0
12, 13 -- Message-Id: {p06240800c748903e15e0-at-[206.69.208.22]}
12, 13 -- Date: Fri, 11 Dec 2009 18:12:07 -0600
12, 13 -- To: microscopy-at-microscopy.com
12, 13 -- From: dridings-at-carolinas.org (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Open TEM Technician Position
12, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
12, 13 -- Content-Transfer-Encoding: 8bit
12, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBC0C8hi011480
==============================End of - Headers==============================

From 453aeec1.4000305-at-asahi-kanri.co.jp Fri Dec 11 18:53:38 2009
Return-Path: {453aeec1.4000305-at-asahi-kanri.co.jp}
Received: from [186.24.22.3] ([186.24.22.3])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBC0ra9O028092;
Fri, 11 Dec 2009 18:53:37 -0600
Received: from [87.59.56.192] (account 453aeec1.4000305-at-asahi-kanri.co.jp HELO hdvpdb.nixsbp.va)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 771309255 for microscopy-at-microscopy.com; Fri, 11 Dec 2009 21:53:36 -0300

A question from someone who definitely should know this answer, but doesn't:

What precisely is the difference between a biological TEM and a materials TEM?

What happens if you put thin sections of a resin-embedded biological
specimen into a TEM
that is used for materials science? Is the main difference what
happens in terms of radiation damage to the specimen? I assume the
biological nature of the specimen doesn't make any difference, but
the thinness of the sections, and the composition of the resin, might
do.


==============================Original Headers==============================
4, 19 -- From gw265-at-cam.ac.uk Sat Dec 12 04:12:34 2009
4, 19 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131])
4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBCACX8v009127
4, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 04:12:34 -0600
4, 19 -- X-Cam-AntiVirus: no malware found
4, 19 -- X-Cam-SpamDetails: not scanned
4, 19 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
4, 19 -- Received: from host81-157-56-89.range81-157.btcentralplus.com ([81.157.56.89]:49289 helo=[192.168.1.70])
4, 19 -- by ppsw-1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:465)
4, 19 -- with esmtpsa (PLAIN:gw265) (TLSv1:DHE-RSA-AES256-SHA:256)
4, 19 -- id 1NJOxk-0001ii-5m (Exim 4.70) for Microscopy-at-microscopy.com
4, 19 -- (return-path {gw265-at-cam.ac.uk} ); Sat, 12 Dec 2009 10:12:32 +0000
4, 19 -- Mime-Version: 1.0
4, 19 -- Message-Id: {f06210200c7491ca0c024-at-[192.168.1.70]}
4, 19 -- Date: Sat, 12 Dec 2009 10:12:28 +0000
4, 19 -- To: Microscopy-at-microscopy.com
4, 19 -- From: Giselle Walker {gw265-at-cam.ac.uk}
4, 19 -- Subject: biological TEM vs. materials TEM?
4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Sat, 12 Dec 2009 08:36:39 -0600
Subject: [Microscopy] Re: biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

While there really isn't any fundamental difference between the two,
there are usually some differences in practice. You can certainly use a
materials scope for a bio sample and vice versa.

Materials scopes tend to use a higher kV (commonly 200kV for materials
vs 80-120kV for bio). The higher Z of most materials samples pushes
materials people to the higher kV range for better penetration. Bio
samples benefit from the greater contrast at the lower kV.

Materials people will want to go for the highest brightness electron
source, while this isn't quite so important (painting with very broad
strokes) for the bio people. Bio samples tend to be more beam sensitive
than most materials samples and burn up at typical materials doses.

Materials people tend to do much more diffraction analysis than the bio
people. Some of the microscopes marketed for the bio people omit the
Selected Area aperture. Also, many of these bio scopes omit the ability
to do sample tilting. This would make those microscopes problematic for
a materials researcher.

You mention the embedding resin used in bio samples. Because of the
analysis we do in my scopes, carbonaceous contamination is high on my
list of concerns. I try to minimize the amount of epoxy going into my
scopes and keep the beam away from the epoxy when I have such a sample.
I only occasionally look at bio samples in my scope, so I'm not too
concerned about them. If they were a large fraction of my samples, I
would invest in an in-column plasma cleaner, so that I wouldn't kill my
capability to to small probe work.

I hope this helps.

Cheers,
Henk

gw265-at-cam.ac.uk wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} A question from someone who definitely should know this answer, but doesn't:
}
} What precisely is the difference between a biological TEM and a materials TEM?
}
} What happens if you put thin sections of a resin-embedded biological
} specimen into a TEM
} that is used for materials science? Is the main difference what
} happens in terms of radiation damage to the specimen? I assume the
} biological nature of the specimen doesn't make any difference, but
} the thinness of the sections, and the composition of the resin, might
} do.
}
}
} ==============================Original Headers==============================
} 4, 19 -- From gw265-at-cam.ac.uk Sat Dec 12 04:12:34 2009
} 4, 19 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131])
} 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBCACX8v009127
} 4, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 04:12:34 -0600
} 4, 19 -- X-Cam-AntiVirus: no malware found
} 4, 19 -- X-Cam-SpamDetails: not scanned
} 4, 19 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/
} 4, 19 -- Received: from host81-157-56-89.range81-157.btcentralplus.com ([81.157.56.89]:49289 helo=[192.168.1.70])
} 4, 19 -- by ppsw1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:465)
} 4, 19 -- with esmtpsa (PLAIN:gw265) (TLSv1:DHE-RSA-AES256-SHA:256)
} 4, 19 -- id 1NJOxk-0001ii-5m (Exim 4.70) for Microscopy-at-microscopy.com
} 4, 19 -- (return-path {gw265-at-cam.ac.uk} ); Sat, 12 Dec 2009 10:12:32 +0000
} 4, 19 -- Mime-Version: 1.0
} 4, 19 -- Message-Id: {f06210200c7491ca0c024-at-[192.168.1.70]}
} 4, 19 -- Date: Sat, 12 Dec 2009 10:12:28 +0000
} 4, 19 -- To: Microscopy-at-microscopy.com
} 4, 19 -- From: Giselle Walker {gw265-at-cam.ac.uk}
} 4, 19 -- Subject: biological TEM vs. materials TEM?
} 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
10, 26 -- From colijn.1-at-osu.edu Sat Dec 12 08:36:39 2009
10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBCEadK6001655
10, 26 -- for {microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 08:36:39 -0600
10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- id {01NH5XQR4P2896AU4F-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
10, 26 -- Sat, 12 Dec 2009 09:36:38 -0500 (EST)
10, 26 -- Received: from [192.168.1.102]
10, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116])
10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
10, 26 -- with ESMTPA id {01NH5XQQLLIY9683TB-at-ecr6.ohio-state.edu} for
10, 26 -- microscopy-at-microscopy.com; Sat, 12 Dec 2009 09:36:38 -0500 (EST)
10, 26 -- Date: Sat, 12 Dec 2009 09:36:37 -0500
10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
10, 26 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
10, 26 -- In-reply-to: {200912121014.nBCAEBpZ011184-at-ns.microscopy.com}
10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
10, 26 -- To: microscopy-at-microscopy.com
10, 26 -- Message-id: {4B23AA75.6080603-at-osu.edu}
10, 26 -- MIME-version: 1.0
10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
10, 26 -- Content-transfer-encoding: 7bit
10, 26 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu
10, 26 -- References: {200912121014.nBCAEBpZ011184-at-ns.microscopy.com}
==============================End of - Headers==============================




From: ropope-at-gmail.com
Date: Mon, 14 Dec 2009 08:34:03 -0600
Subject: [Microscopy] viaWWW: Question about Image Database Programs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Great question!

The differences between a TEM used primarily for biological
investigations (biological TEM) and a TEM used principally for
materials work (materials TEM) has to do with convenience.

Both instruments could be used to examine either type of specimen, but
they are configured to expedite the work in one or the other mode. For
example, using specialized diffraction procedures on a conventional,
biological TEM requires a lot of instrument tweaking (primarily
alignment), whereas, the same procedure could be as simple as pushing
a button on a materials TEM.

At the risk of making a sweeping generalization (but probably
stimulating some discussion), I would say that a conventional,
biological TEM could be regarded as a basic instrument, whereas a
materials TEM would be regarded as having more capabilities. Most
biological studies have to do with capturing images while materials
studies have to perform analyses of the specimens as well as capture
images.

Here is a summary of the major differences in terms of capabilities:

Biological TEM: basic imaging, high contrast, very low magnification
(50x or so) with good contrast, good resolution (1 nm or so), tilting
and possibly rotation of specimen, good vacuum, primarily tungsten
filament but also able to use LaB6 in newer ones, generally operated
at 60-80 kV.

Materials TEM: high resolution (better than 1 nm), bright gun by means
of LaB6 or possibly field emission, able to orientate specimen in many
ways (tilt, rotate, tilt and rotate, eucentric), advanced analytical
capability (specialized diffraction, X-ray, EELS) possibly with STEM
attachment, highest and clean vacuum (turbo, ion, cryo), generally
operated at 100 kV or higher.

So, maybe a better way of characterizing the two instruments would
be: basic imaging versus analytical TEM's, since biologists could make
use of the analytical features.

It is safe to say that biologists would probably be satisfied with
either instrument, but materials researchers would require one with
more advanced capability.

I shall now retreat to my fortified saferoom.


}
} What precisely is the difference between a biological TEM and a materials TEM?
}
} What happens if you put thin sections of a resin-embedded biological
} specimen into a TEM
} that is used for materials science?  Is the main difference what
} happens in terms of radiation damage to the specimen? I assume the
} biological nature of the specimen doesn't make any difference, but
} the thinness of the sections, and the composition of the resin, might
} do.


--
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901
Phone: 618-453-3730


==============================Original Headers==============================
15, 19 -- From bozzola-at-siu.edu Sat Dec 12 11:01:48 2009
15, 19 -- Received: from mail-gx0-f221.google.com (mail-gx0-f221.google.com [209.85.217.221])
15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBCH1mJo021134
15, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 11:01:48 -0600
15, 19 -- Received: by gxk21 with SMTP id 21so139994gxk.10
15, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 09:01:47 -0800 (PST)
15, 19 -- MIME-Version: 1.0
15, 19 -- Received: by 10.150.110.23 with SMTP id i23mr4396202ybc.345.1260637307797;
15, 19 -- Sat, 12 Dec 2009 09:01:47 -0800 (PST)
15, 19 -- In-Reply-To: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- References: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- Date: Sat, 12 Dec 2009 11:01:47 -0600
15, 19 -- Message-ID: {ebc2299e0912120901k6cd051afkde86994f67640196-at-mail.gmail.com}
15, 19 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
15, 19 -- From: John Bozzola {bozzola-at-siu.edu}
15, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
15, 19 -- Content-Type: text/plain; charset=UTF-8
15, 19 -- Content-Transfer-Encoding: 8bit
15, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBCH1mJo021134
==============================End of - Headers==============================

From aa234567-at-ms21.hinet.net Sat Dec 12 15:09:48 2009
Return-Path: {aa234567-at-ms21.hinet.net}
Received: from cpe-17.160.55.190.in-addr.arpa (cpe-17.160.55.190.in-addr.arpa [190.55.160.17] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBCL9jv5011873;
Sat, 12 Dec 2009 15:09:46 -0600
Received: from [100.172.159.132] (account amano-at-nissay-nbs.co.jp HELO qsjrymvnyuqzc.lwphiysv.ru)
by cpe-17.160.55.190.in-addr.arpa (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 588733175 for microscopy-at-microscopy.com; Sat, 12 Dec 2009 18:09:45 -0300

I think you are both missing an important area of biological TEM:
Cryo imaging and diffraction for structural biology.

In this field we use 120 - 300 kV, magnifications between 40000 and 100000,
and we reach resolution better than 0.3 nm for protein crystals and around
0.5 nm for noncrystalline specimens.

Most of the microscopes are equipped with field emission guns for the high
spatial coherence.

Good contrast at low spatial frequencies is normally achieved by
defocussing, not by low voltage.

Being able to cool the specimen to the temperature of liquid nitrogen
or even helium is one of the essential capabilities of our microscopes.

You can come out of your fortified saferoom again. I've finished. :)

Philip


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: 12 December 2009 18:08
To: Philip.Koeck-at-ki.se

Great question!

The differences between a TEM used primarily for biological
investigations (biological TEM) and a TEM used principally for
materials work (materials TEM) has to do with convenience.

Both instruments could be used to examine either type of specimen, but
they are configured to expedite the work in one or the other mode. For
example, using specialized diffraction procedures on a conventional,
biological TEM requires a lot of instrument tweaking (primarily
alignment), whereas, the same procedure could be as simple as pushing
a button on a materials TEM.

At the risk of making a sweeping generalization (but probably
stimulating some discussion), I would say that a conventional,
biological TEM could be regarded as a basic instrument, whereas a
materials TEM would be regarded as having more capabilities. Most
biological studies have to do with capturing images while materials
studies have to perform analyses of the specimens as well as capture
images.

Here is a summary of the major differences in terms of capabilities:

Biological TEM: basic imaging, high contrast, very low magnification
(50x or so) with good contrast, good resolution (1 nm or so), tilting
and possibly rotation of specimen, good vacuum, primarily tungsten
filament but also able to use LaB6 in newer ones, generally operated
at 60-80 kV.

Materials TEM: high resolution (better than 1 nm), bright gun by means
of LaB6 or possibly field emission, able to orientate specimen in many
ways (tilt, rotate, tilt and rotate, eucentric), advanced analytical
capability (specialized diffraction, X-ray, EELS) possibly with STEM
attachment, highest and clean vacuum (turbo, ion, cryo), generally
operated at 100 kV or higher.

So, maybe a better way of characterizing the two instruments would
be: basic imaging versus analytical TEM's, since biologists could make
use of the analytical features.

It is safe to say that biologists would probably be satisfied with
either instrument, but materials researchers would require one with
more advanced capability.

I shall now retreat to my fortified saferoom.


}
} What precisely is the difference between a biological TEM and a materials
TEM?
}
} What happens if you put thin sections of a resin-embedded biological
} specimen into a TEM
} that is used for materials science?  Is the main difference what
} happens in terms of radiation damage to the specimen? I assume the
} biological nature of the specimen doesn't make any difference, but
} the thinness of the sections, and the composition of the resin, might
} do.


--
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL 62901
Phone: 618-453-3730


==============================Original Headers==============================
15, 19 -- From bozzola-at-siu.edu Sat Dec 12 11:01:48 2009
15, 19 -- Received: from mail-gx0-f221.google.com (mail-gx0-f221.google.com
[209.85.217.221])
15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nBCH1mJo021134
15, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 11:01:48
-0600
15, 19 -- Received: by gxk21 with SMTP id 21so139994gxk.10
15, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 09:01:47
-0800 (PST)
15, 19 -- MIME-Version: 1.0
15, 19 -- Received: by 10.150.110.23 with SMTP id
i23mr4396202ybc.345.1260637307797;
15, 19 -- Sat, 12 Dec 2009 09:01:47 -0800 (PST)
15, 19 -- In-Reply-To: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- References: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- Date: Sat, 12 Dec 2009 11:01:47 -0600
15, 19 -- Message-ID:
{ebc2299e0912120901k6cd051afkde86994f67640196-at-mail.gmail.com}
15, 19 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
15, 19 -- From: John Bozzola {bozzola-at-siu.edu}
15, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
15, 19 -- Content-Type: text/plain; charset=UTF-8
15, 19 -- Content-Transfer-Encoding: 8bit
15, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nBCH1mJo021134
==============================End of - Headers==============================




==============================Original Headers==============================
31, 23 -- From Philip.Koeck-at-ki.se Mon Dec 14 03:05:44 2009
31, 23 -- Received: from smtp6.ki.se (smtp6.ki.se [130.237.98.104])
31, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBE95h34024531
31, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 03:05:44 -0600
31, 23 -- Received: from Rapana (rapana.csb.ki.se [130.237.109.49])
31, 23 -- by smtp6.ki.se (Postfix) with ESMTP id 1025CD4BDB;
31, 23 -- Mon, 14 Dec 2009 10:05:43 +0100 (CET)
31, 23 -- From: "Philip Koeck" {Philip.Koeck-at-ki.se}
31, 23 -- To: {microscopy-at-microscopy.com}
31, 23 -- Cc: {bozzola-at-siu.edu} , {colijn.1-at-osu.edu}
31, 23 -- References: {200912121708.nBCH8HYm032360-at-ns.microscopy.com}
31, 23 -- Subject: Re: biological TEM vs. materials TEM?
31, 23 -- Date: Mon, 14 Dec 2009 10:05:36 +0100
31, 23 -- Message-ID: {C01A49B925E7439B9EE6F9A0D941C2CB-at-ad.biosci.ki.se}
31, 23 -- MIME-Version: 1.0
31, 23 -- Content-Type: text/plain;
31, 23 -- charset="iso-8859-1"
31, 23 -- X-Mailer: Microsoft Office Outlook 11
31, 23 -- In-reply-to: {200912121708.nBCH8HYm032360-at-ns.microscopy.com}
31, 23 -- Thread-index: Acp7TbQ7KOZ0F4WGRAajzRvzURkgiABTQMlQ
31, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
31, 23 -- Content-Transfer-Encoding: 8bit
31, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBE95h34024531
==============================End of - Headers==============================

From c011-at-jobmail.evta.gov.tw Mon Dec 14 03:57:56 2009
Return-Path: {c011-at-jobmail.evta.gov.tw}
Received: from host15-54-static.104-82-b.business.telecomitalia.it (host15-54-static.104-82-b.business.telecomitalia.it [82.104.54.15])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBE9vpXw008491;
Mon, 14 Dec 2009 03:57:52 -0600
Received: from [46.68.99.77] (account aire53-at-ms29.hinet.net HELO zkhjnoyxk.hcgjvexpksknh.tv)
by host15-54-static.104-82-b.business.telecomitalia.it (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 023635662 for microscopy-at-microscopy.com; Mon, 14 Dec 2009 10:57:52 +0100

As the material scientists pointed out, a "basic" microscope (as described by John) would USUALLY suffice for MOST of biological research. I used to know a good scientist who made his whole career with a tungsten 80kV microscope without digital camera (although he needs collaborations from time to time, esp. for EMtomography work).

BUT, as Philip said, some projects in biology require much more advanced microscope capabilities and I would just add 2 options to the ones described by Philip:
- Sample tilting (and high voltage) is required for EM tomography (3D reconstruction).
- STEM and EDX are required for element mapping (for pathologists f.ex.).

In summary I would say that "biological TEM" has no straightforward definition, because biology is a vast field with plenty of applications (\ironical mode on\ in contrast to material science \ironical mode off\). Of course, if you can afford it, buy a FE 300kV with double tilt, EMtomography, crystallogry, cryo, STEM and EDX and it will make everyone happy!

regards

Stephane




----- Original Message ----
X-from: "Philip.Koeck-at-ki.se" {Philip.Koeck-at-ki.se}
To: nizets2-at-yahoo.com
Sent: Mon, December 14, 2009 10:11:17 AM

I think you are both missing an important area of biological TEM:
Cryo imaging and diffraction for structural biology.

In this field we use 120 - 300 kV, magnifications between 40000 and 100000,
and we reach resolution better than 0.3 nm for protein crystals and around
0.5 nm for noncrystalline specimens.

Most of the microscopes are equipped with field emission guns for the high
spatial coherence.

Good contrast at low spatial frequencies is normally achieved by
defocussing, not by low voltage.

Being able to cool the specimen to the temperature of liquid nitrogen
or even helium is one of the essential capabilities of our microscopes.

You can come out of your fortified saferoom again. I've finished. :)

Philip


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: 12 December 2009 18:08
To: Philip.Koeck-at-ki.se

Great question!

The differences between a TEM used primarily for biological
investigations (biological TEM) and a TEM used principally for
materials work (materials TEM) has to do with convenience.

Both instruments could be used to examine either type of specimen, but
they are configured to expedite the work in one or the other mode. For
example, using specialized diffraction procedures on a conventional,
biological TEM requires a lot of instrument tweaking (primarily
alignment), whereas, the same procedure could be as simple as pushing
a button on a materials TEM.

At the risk of making a sweeping generalization (but probably
stimulating some discussion), I would say that a conventional,
biological TEM could be regarded as a basic instrument, whereas a
materials TEM would be regarded as having more capabilities. Most
biological studies have to do with capturing images while materials
studies have to perform analyses of the specimens as well as capture
images.

Here is a summary of the major differences in terms of capabilities:

Biological TEM: basic imaging, high contrast, very low magnification
(50x or so) with good contrast, good resolution (1 nm or so), tilting
and possibly rotation of specimen, good vacuum, primarily tungsten
filament but also able to use LaB6 in newer ones, generally operated
at 60-80 kV.

Materials TEM: high resolution (better than 1 nm), bright gun by means
of LaB6 or possibly field emission, able to orientate specimen in many
ways (tilt, rotate, tilt and rotate, eucentric), advanced analytical
capability (specialized diffraction, X-ray, EELS) possibly with STEM
attachment, highest and clean vacuum (turbo, ion, cryo), generally
operated at 100 kV or higher.

So, maybe a better way of characterizing the two instruments would
be: basic imaging versus analytical TEM's, since biologists could make
use of the analytical features.

It is safe to say that biologists would probably be satisfied with
either instrument, but materials researchers would require one with
more advanced capability.

I shall now retreat to my fortified saferoom.


}
} What precisely is the difference between a biological TEM and a materials
TEM?
}
} What happens if you put thin sections of a resin-embedded biological
} specimen into a TEM
} that is used for materials science?  Is the main difference what
} happens in terms of radiation damage to the specimen? I assume the
} biological nature of the specimen doesn't make any difference, but
} the thinness of the sections, and the composition of the resin, might
} do.


--
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901
Phone: 618-453-3730


==============================Original Headers==============================
15, 19 -- From bozzola-at-siu.edu Sat Dec 12 11:01:48 2009
15, 19 -- Received: from mail-gx0-f221.google.com (mail-gx0-f221.google.com
[209.85.217.221])
15, 19 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nBCH1mJo021134
15, 19 --     for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 11:01:48
-0600
15, 19 -- Received: by gxk21 with SMTP id 21so139994gxk.10
15, 19 --        for {Microscopy-at-microscopy.com} ; Sat, 12 Dec 2009 09:01:47
-0800 (PST)
15, 19 -- MIME-Version: 1.0
15, 19 -- Received: by 10.150.110.23 with SMTP id
i23mr4396202ybc.345.1260637307797;
15, 19 --     Sat, 12 Dec 2009 09:01:47 -0800 (PST)
15, 19 -- In-Reply-To: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- References: {200912121013.nBCADcW0010386-at-ns.microscopy.com}
15, 19 -- Date: Sat, 12 Dec 2009 11:01:47 -0600
15, 19 -- Message-ID:
{ebc2299e0912120901k6cd051afkde86994f67640196-at-mail.gmail.com}
15, 19 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
15, 19 -- From: John Bozzola {bozzola-at-siu.edu}
15, 19 -- To: MSAListserver {Microscopy-at-microscopy.com}
15, 19 -- Content-Type: text/plain; charset=UTF-8
15, 19 -- Content-Transfer-Encoding: 8bit
15, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nBCH1mJo021134
==============================End of - Headers==============================




==============================Original Headers==============================
31, 23 -- From Philip.Koeck-at-ki.se Mon Dec 14 03:05:44 2009
31, 23 -- Received: from smtp6.ki.se (smtp6.ki.se [130.237.98.104])
31, 23 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBE95h34024531
31, 23 --     for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 03:05:44 -0600
31, 23 -- Received: from Rapana (rapana.csb.ki.se [130.237.109.49])
31, 23 --     by smtp6.ki.se (Postfix) with ESMTP id 1025CD4BDB;
31, 23 --     Mon, 14 Dec 2009 10:05:43 +0100 (CET)
31, 23 -- From: "Philip Koeck" {Philip.Koeck-at-ki.se}
31, 23 -- To: {microscopy-at-microscopy.com}
31, 23 -- Cc: {bozzola-at-siu.edu} , {colijn.1-at-osu.edu}
31, 23 -- References: {200912121708.nBCH8HYm032360-at-ns.microscopy.com}
31, 23 -- Subject: Re: biological TEM vs. materials TEM?
31, 23 -- Date: Mon, 14 Dec 2009 10:05:36 +0100
31, 23 -- Message-ID: {C01A49B925E7439B9EE6F9A0D941C2CB-at-ad.biosci.ki.se}
31, 23 -- MIME-Version: 1.0
31, 23 -- Content-Type: text/plain;
31, 23 --     charset="iso-8859-1"
31, 23 -- X-Mailer: Microsoft Office Outlook 11
31, 23 -- In-reply-to: {200912121708.nBCH8HYm032360-at-ns.microscopy.com}
31, 23 -- Thread-index: Acp7TbQ7KOZ0F4WGRAajzRvzURkgiABTQMlQ
31, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579
31, 23 -- Content-Transfer-Encoding: 8bit
31, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBE95h34024531
==============================End of - Headers==============================






==============================Original Headers==============================
50, 24 -- From nizets2-at-yahoo.com Mon Dec 14 04:46:56 2009
50, 24 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237])
50, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBEAktqJ011090
50, 24 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 04:46:55 -0600
50, 24 -- Received: (qmail 89277 invoked by uid 60001); 14 Dec 2009 10:46:55 -0000
50, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1260787615; bh=sBUhN9hzKnpqohervazZUkJDJQqembIIok9/GLC1ZWA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=wlIQx4Kx+l2iflvuK3dDjip/ayuNetxqMxVSLjtxCIa7vKfSZIonA73qPfHXegSdn7RZF2ociiw53hRVxJvzUhgJ9fZTVHV/nQnHXHR5BSgFvROEaGFVKgiiMZtGchVrJRXoXkg3Me+SxAe7dyEObyLK3elofNj+kMJKD6SClwU=
50, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
50, 24 -- s=s1024; d=yahoo.com;
50, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
50, 24 -- b=hjfUQv5sMFyV50kExxs6tzB++MbLU1L5GcSJUobuV9TnTnAqWVxerysncl72kENFIV6BrcB1gwug7PYINcEHHuYlkTTf6Dy6BkIeNV0F6HKN0htkbxIKp8NBZStvA2+yjBvwYqeqMPqSr2GHWd63szKoL3A+Q2As80TGep+KVo0=;
50, 24 -- Message-ID: {214039.87795.qm-at-web110814.mail.gq1.yahoo.com}
50, 24 -- X-YMail-OSG: f45so78VM1n4IYfToXEdlymkDe_42zyeI3lMr35Hr1zZ1OQmOBdeCgV55L9FeL51AR17X0Bxg_y_F.SlYuTp9Q1eVx1BzloRxsWWQHyoYPKm4tTGji2ybi37lyzvMP35_x3vLjdebcJOKUfBMFGaBRCOkNO1R7OZ.Q6_jWpQobAuwrKcdMyCR._Wpt7V2r_q0uteaVLabUabOFieBQo.jreU9AMks8JCnABqiWCOd.sRNmmcpHFgoQggvScUg8WgEX6P5c.oVPb1ucF9Jz9UG5PWd.862.ufNE_pq.AcssuBp8M2lsV7TzC4j4GxM3Xe1vcQ39_66mv5dnUL.GxtgPc53MHcYRd4MXN92TcgB_g_uxDLtwoccueMt78UjJltjrtAFVbfRMzHxTsIzKVaRjFfjrMkWxHd41eM8090jyigiGfIdKOkHVQ88aG8TDFDeJO3N2SpyX.4HQ1YuVGvnBGEx1BB8xZ90K_zrCVEuF_rqva6pwZbMDdAk9sJJ904CX9DooLyNTALZrDmYjamSpOnMfzArLoT7wjR5zoISjIbdl77tT5eVQczPwUStQTYZHui.f7RGg_96kGN
50, 24 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Mon, 14 Dec 2009 02:46:55 PST
50, 24 -- X-Mailer: YahooMailRC/240.3 YahooMailWebService/0.8.100.260964
50, 24 -- References: {200912140911.nBE9BHFX030797-at-ns.microscopy.com}
50, 24 -- Date: Mon, 14 Dec 2009 02:46:55 -0800 (PST)
50, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
50, 24 -- Subject: Re: [Microscopy] Re: biological TEM vs. materials TEM?
50, 24 -- To: microscopy-at-microscopy.com
50, 24 -- In-Reply-To: {200912140911.nBE9BHFX030797-at-ns.microscopy.com}
50, 24 -- MIME-Version: 1.0
50, 24 -- Content-Type: text/plain; charset=iso-8859-1
50, 24 -- Content-Transfer-Encoding: 8bit
50, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBEAktqJ011090
==============================End of - Headers==============================

From ahecdd-at-ms2.hinet.net Mon Dec 14 05:39:57 2009
Return-Path: {ahecdd-at-ms2.hinet.net}
Received: from [114.143.48.120] ([114.143.48.120])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEBdujm027017;
Mon, 14 Dec 2009 05:39:57 -0600
Received: from [197.62.131.142] (account ahecdd-at-ms2.hinet.net HELO zymotptzaylkzb.waoyey.com)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 891854374 for microscopy-at-microscopy.com; Mon, 14 Dec 2009 17:09:56 +0530

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ropope-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ropope-at-gmail.com
Name: Robert Pope

Organization: BNBI

Title-Subject: [Filtered] Question about Image Database Programs

Question: Good Day Everyone:
I have monitored the microscopy listserve for many years and only
reply to threads when I think I have meaningful insight. I have a
question for all the experts in the field. I have developed image
archives in the past, but would like to set up a new image database
that is searchable. I have been working with FileMaker Pro and have
had good results, but wanted to see if any of you have had luck with
other programs that are off the shelf. I realize building your own
database and writing code tends to work best, but I need to use an
off the shelf product for this specific project. If you have any
meaningful comments, I would be most grateful to read them.
Thank you,
Robert

Robert K. Pope, Ph.D.
Principal Investigator
Electron Microscopy Laboratory Manager
ropope-at-gmail.com

I remember when green screen was a circle of beautiful special
effects in the TEM (seeing what no one has seen before), and not a
mechanism for special effects in the theater.


Login Host: 12.198.101.3
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 08:34:03 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEEY0j1032519
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 08:34:02 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c74bfd45a785-at-[206.69.208.22]}
9, 11 -- Date: Mon, 14 Dec 2009 08:34:00 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: ropope-at-gmail.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Question about Image Database Programs
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 14 Dec 2009 08:46:25 -0600
Subject: [Microscopy] Re: biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

{wishy-washy} I have to agree with most of the
biological vs materials statements, but ...
{/wishy-washy} (if anyone still codes web pages)
... mostly, I find the whole issue a false
dichotomy. Think of polymers. Embedding resins
are polymers with (one hopes) interesting
impurities (the biological sample). Or better,
biological materials are mostly complex, hydrated
polymers. Materials, that is.

The *real* difference between biological and
materials microscopy (note the end "y", not "e",
therefore a thread hijack) is in the specimen
preparation. Not in the microscope, really. The
difference between biological and materials
microscopes (back to "e") is in the added gadgets
and analytical capabilities, the selection being
driven by the questions asked. But as long as the
given instrument can generate the data needed,
whether it is a "biological" or "materials" TEM
depends on whether it is being operated by a
biologist or materials scientist.
And if the person is a biomaterials person, well ...

Phil

} As the material scientists pointed out, a
} "basic" microscope (as described by John) would
} USUALLY suffice for MOST of biological research.
} I used to know a good scientist who made his
} whole career with a tungsten 80kV microscope
} without digital camera (although he needs
} collaborations from time to time, esp. for
} EMtomography work).
}
} BUT, as Philip said, some projects in biology
} require much more advanced microscope
} capabilities and I would just add 2 options to
} the ones described by Philip:
} - Sample tilting (and high voltage) is required
} for EM tomography (3D reconstruction).
} - STEM and EDX are required for element mapping (for pathologists f.ex.).
}
} In summary I would say thatÝ"biological TEM" has
} no straightforward definition, because biology
} is a vast field with plenty of applications
} (\ironical mode on\ in contrast to material
} science \ironical mode off\). Of course, if you
} can afford it, buy a FE 300kV with double tilt,
} EMtomography, crystallogry, cryo, STEM and EDX
} and it will make everyone happy!
}
} regards
}
} Stephane
}
}
}
}
} ----- Original Message ----
} X-from: "Philip.Koeck-at-ki.se" {Philip.Koeck-at-ki.se}
} To: nizets2-at-yahoo.com
} Sent: Mon, December 14, 2009 10:11:17 AM
} Subject: [Microscopy] Re: biological TEM vs. materials TEM?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of America

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
6, 27 -- From oshel1pe-at-cmich.edu Mon Dec 14 08:46:25 2009
6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEEkOim015330
6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 08:46:24 -0600
6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEEjhPY010794
6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:46:21 -0500
6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Mon, 14 Dec 2009 09:45:54 -0500
6, 27 -- Mime-Version: 1.0
6, 27 -- Message-Id: {f06240804c74bfe2ed475-at-[141.209.160.249]}
6, 27 -- In-Reply-To: {200912141050.nBEAoDGu016661-at-ns.microscopy.com}
6, 27 -- References: {200912141050.nBEAoDGu016661-at-ns.microscopy.com}
6, 27 -- Date: Mon, 14 Dec 2009 09:45:52 -0500
6, 27 -- To: Microscopy-at-microscopy.com
6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 27 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
6, 27 -- X-OriginalArrivalTime: 14 Dec 2009 14:45:55.0131 (UTC) FILETIME=[23EBD0B0:01CA7CCC]
6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 27 -- X-Spam-Score: -2.40 () [Hold at 6.00] L_EXCH_MF,L_USD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5)
6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 27 -- X-CanItPRO-Stream: default
6, 27 -- X-Canit-Stats-ID: 24407981 - d8f037cb6412 - 20091214
6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
6, 27 -- Content-Transfer-Encoding: 8bit
6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBEEkOim015330
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Mon, 14 Dec 2009 08:47:30 -0600
Subject: [Microscopy] Should I replace retired EDS detector?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm seeking advice. Our service contractor just informed me that they are
retiring the EDS detector on our Cambridge Stereoscan 200 SEM (Circa 1984)
effective at the expiration of our current contract in February. They will
no longer support the detector after that time. Should I consider an upgrade
on such a vintage instrument or just continue without the service contract?

Thanks,

Jeff Stewart
Metallographic Lab Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329


==============================Original Headers==============================
4, 40 -- From SRS0=KIwxxE=IA=metallography.com=jeff-at-eigbox.net Mon Dec 14 08:47:29 2009
4, 40 -- Received: from bosmailout01.eigbox.net (bosmailout01.eigbox.net [66.96.186.1])
4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEElT36017384
4, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 08:47:29 -0600
4, 40 -- Received: from bosmailscan24.eigbox.net ([10.20.15.24])
4, 40 -- by bosmailout01.eigbox.net with esmtp (Exim)
4, 40 -- id 1NKCCu-0001bN-LS
4, 40 -- for microscopy-at-microscopy.com; Mon, 14 Dec 2009 09:47:28 -0500
4, 40 -- Received: from bosimpout02.eigbox.net ([10.20.55.2])
4, 40 -- by bosmailscan24.eigbox.net with esmtp (Exim)
4, 40 -- id 1NKCCu-00011W-Bw
4, 40 -- for microscopy-at-microscopy.com; Mon, 14 Dec 2009 09:47:28 -0500
4, 40 -- Received: from bosauthsmtp02.eigbox.net ([10.20.18.2])
4, 40 -- by bosimpout02.eigbox.net with NO UCE
4, 40 -- id H2mx1d00M02gpmq012mxeY; Mon, 14 Dec 2009 09:46:57 -0500
4, 40 -- X-EN-OrigOutIP: 10.20.18.2
4, 40 -- X-EN-IMPSID: H2mx1d00M02gpmq012mxeY
4, 40 -- Received: from [64.17.227.194] (helo=jstewart)
4, 40 -- by bosauthsmtp02.eigbox.net with esmtpa (Exim)
4, 40 -- id 1NKCCu-00054j-Dg
4, 40 -- for Microscopy-at-microscopy.com; Mon, 14 Dec 2009 09:47:28 -0500
4, 40 -- Message-ID: {001301ca7ccd$41c74a00$461510ac-at-sternmark.local}
4, 40 -- Reply-To: "Jeff Stewart" {jeff-at-metallography.com}
4, 40 -- From: "Jeff Stewart" {jeff-at-metallography.com}
4, 40 -- To: {Microscopy-at-microscopy.com}
4, 40 -- Subject: Should I replace retired EDS detector?
4, 40 -- Date: Mon, 14 Dec 2009 09:53:52 -0500
4, 40 -- MIME-Version: 1.0
4, 40 -- Content-Type: text/plain;
4, 40 -- charset="iso-8859-1"
4, 40 -- Content-Transfer-Encoding: 7bit
4, 40 -- X-Priority: 3
4, 40 -- X-MSMail-Priority: Normal
4, 40 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1106
4, 40 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1106
4, 40 -- X-EN-UserInfo: e86f17d72db2b073c5072c937c9a2a3f:f6ee1f837e2a1583e399b28f5cf1cc41
4, 40 -- X-EN-AuthUser: jeff-at-metallography.com
4, 40 -- Sender: "Jeff Stewart" {jeff-at-metallography.com}
4, 40 -- X-EN-OrigIP: 64.17.227.194
4, 40 -- X-EN-OrigHost: unknown
==============================End of - Headers==============================




From: D.L.Bayliss-at-lboro.ac.uk
Date: Mon, 14 Dec 2009 09:28:30 -0600
Subject: [Microscopy] SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Need some advice on the best method to go with for the preparation of my biological samples for SEM.

My samples are prepared by filtrating my bacterial suspension onto nucleopore track etched membrane filters. After the bacteria have been filtered the membranes are left on agar slabs to dry for 1 hour so the bacteria can be well attached.

The bacteria on the membrane will be treated with cold atmospheric plasma to inactivate the bacteria. I need to use SEM to see how the bacteria are depositing on the membrane filters at my desired bacterial concentration.

The Current preparation protocol procedure I have been using is as follows:

1) Filter bacterial suspension on to membrane and allow to dry/attach for 1 hour.

2) Stick the membrane filtered bacteria onto a SEM stub with a Carbon sticky pad.

3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.

4) Wash in distilled water then grade up to absolute ethanol 20% - 100%

5) Grade fully dehydrated sample in amyl acetate up to 100%.

6) CPD the samples using liquid CO2 passing it through its critical point.

The problems I'm having with my method is the best way to secure the membrane down for the critical point drying because the carbon sticky pad attachment to the stub works fine until I grade the sample in Amyl acetate then the sticky pad starts to detach from the membrane.

Is it ok to just use ethanol even though it doesn't mix as well with liquid CO2 and just run a longer time with more flushes in the CPD?

Does anyone have any easier suggestions for securing the membrane down so the membrane doesn't get damaged or effected during the dehydration and CPD stages?

Thanks

Danny





==============================Original Headers==============================
20, 37 -- From D.L.Bayliss-at-lboro.ac.uk Mon Dec 14 09:28:30 2009
20, 37 -- Received: from weed.lut.ac.uk (weed.lut.ac.uk [158.125.1.226])
20, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEFSUai013084
20, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:28:30 -0600
20, 37 -- Received: from [158.125.1.203] (helo=ping.lut.ac.uk)
20, 37 -- by weed.lut.ac.uk with esmtps (SSLv3:DES-CBC3-SHA:168)
20, 37 -- (Exim 4.66)
20, 37 -- id 1NKCqb-00080x-79
20, 37 -- for Microscopy-at-microscopy.com; Mon, 14 Dec 2009 15:28:29 +0000
20, 37 -- Received: from [10.23.44.7] (HELO lboro.ac.uk)
20, 37 -- by ping.lboro.ac.uk (CommuniGate Pro SMTP 5.2.6)
20, 37 -- with ESMTPS id 31408001 for Microscopy-at-microscopy.com; Mon, 14 Dec 2009 15:28:29 +0000
20, 37 -- X-Lboro-Archived: Archived by frim
20, 37 -- Received: from [158.125.50.23] (account eldlb-at-lboro.ac.uk)
20, 37 -- by frim.lboro.ac.uk (CommuniGate Pro IMAP 5.2.6)
20, 37 -- with XMIT id 8022202 for Microscopy-at-microscopy.com; Mon, 14 Dec 2009 15:28:28 +0000
20, 37 -- Subject: SEM bacterial sample prep
20, 37 -- Date: Mon, 14 Dec 2009 15:28:27 +0000
20, 37 -- Message-Id: {8cbe9b6ecaa39e4a9d618bae007f7741-at-staff-mail.lboro.ac.uk}
20, 37 -- MIME-Version: 1.0
20, 37 -- Thread-Topic: SEM bacterial sample prep
20, 37 -- Priority: Normal
20, 37 -- Importance: normal
20, 37 -- X-MSMail-Priority: normal
20, 37 -- X-Priority: 3
20, 37 -- Sensitivity: Normal
20, 37 -- Thread-Index: Acp80hT5ARRQGkLLTAipTr2ngRh/sA==
20, 37 -- From: "D.L.Bayliss-at-lboro.ac.uk" {D.L.Bayliss-at-lboro.ac.uk}
20, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
20, 37 -- X-MAPI-LastModified: Mon, 14 Dec 2009 15:28:27 +0000
20, 37 -- X-Mailer: CommuniGate Pro MAPI Connector 1.52.5.1/1.52.5.2(local)
20, 37 -- Content-Type: text/plain;
20, 37 -- charset="us-ascii"
20, 37 -- X-Scan-Signature: 3ea8e91e4a9d56d8510592c33e01bc5f
20, 37 -- X-Lboro-Filtered: weed.lut.ac.uk, Mon, 14 Dec 2009 15:28:29 +0000
20, 37 -- Content-Transfer-Encoding: 8bit
20, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBEFSUai013084
==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Mon, 14 Dec 2009 09:28:39 -0600
Subject: [Microscopy] Re: biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Phil and Stephane,

I knew I was oversimplifying and did not mean to malign my bio
colleagues. There is no way I could dream of doing the work they do.
Give me my nice, robust, high-contrast, simple-to-prepare, radiation
resistant materials samples any day!

I did pass over the work the cryo-tomography people are doing. It is
really exciting stuff. Doing low-dose, high-resolution imaging on
low-contrast samples is not a task for the faint hearted.

I should point out that the microscope companies *market* microscopes
with different capabilities to the bio community. *Most* bio people
don't do diffraction work and I have seen "bio" scopes without an SA
aperture. These tend to have longer focal length (lower ultimate
resolution) objective lenses to improve the contrast of the bio samples.
It seems that, in general, contrast is more of an issue than
*ultimate* resolution. Tomography is still not common in the bio (or
materials) communities. EDX is less common in the bio community than in
the materials, though an increasing number of bio people are now doing
EELS and spectrum imaging. If you walk into a bio EM lab, I don't think
you will find too many STEM capable systems and I think that STEM is a
neglected technique by much of the bio community. The contrast
improvement in STEM seems worthwhile, though I'd be somewhat concerned
about the sample damage.

I didn't wear my asbestos garments today, so go ahead and flame away!

Cheers,
Henk

nizets2-at-yahoo.com wrote:
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} As the material scientists pointed out, a "basic" microscope (as described by John) would USUALLY suffice for MOST of biological research. I used to know a good scientist who made his whole career with a tungsten 80kV microscope without digital camera (although he needs collaborations from time to time, esp. for EMtomography work).
}
} BUT, as Philip said, some projects in biology require much more advanced microscope capabilities and I would just add 2 options to the ones described by Philip:
} - Sample tilting (and high voltage) is required for EM tomography (3D reconstruction).
} - STEM and EDX are required for element mapping (for pathologists f.ex.).
}
} In summary I would say that "biological TEM" has no straightforward definition, because biology is a vast field with plenty of applications (\ironical mode on\ in contrast to material science \ironical mode off\). Of course, if you can afford it, buy a FE 300kV with double tilt, EMtomography, crystallogry, cryo, STEM and EDX and it will make everyone happy!
}
} regards
}
} Stephane
}
}
}
}
} ----- Original Message ----
} X-from: "Philip.Koeck-at-ki.se" {Philip.Koeck-at-ki.se}
} To: nizets2-at-yahoo.com
} Sent: Mon, December 14, 2009 10:11:17 AM
} Subject: [Microscopy] Re: biological TEM vs. materials TEM?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I think you are both missing an important area of biological TEM:
} Cryo imaging and diffraction for structural biology.
}
} In this field we use 120 - 300 kV, magnifications between 40000 and 100000,
} and we reach resolution better than 0.3 nm for protein crystals and around
} 0.5 nm for noncrystalline specimens.
}
} Most of the microscopes are equipped with field emission guns for the high
} spatial coherence.
}
} Good contrast at low spatial frequencies is normally achieved by
} defocussing, not by low voltage.
}
} Being able to cool the specimen to the temperature of liquid nitrogen
} or even helium is one of the essential capabilities of our microscopes.
}
} You can come out of your fortified saferoom again. I've finished. :)
}
} Philip
}
}
} -----Original Message-----
} X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
} Sent: 12 December 2009 18:08
} To: Philip.Koeck-at-ki.se
} Subject: [Microscopy] Re: biological TEM vs. materials TEM?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Great question!
}
} The differences between a TEM used primarily for biological
} investigations (biological TEM) and a TEM used principally for
} materials work (materials TEM) has to do with convenience.
}
} Both instruments could be used to examine either type of specimen, but
} they are configured to expedite the work in one or the other mode. For
} example, using specialized diffraction procedures on a conventional,
} biological TEM requires a lot of instrument tweaking (primarily
} alignment), whereas, the same procedure could be as simple as pushing
} a button on a materials TEM.
}
} At the risk of making a sweeping generalization (but probably
} stimulating some discussion), I would say that a conventional,
} biological TEM could be regarded as a basic instrument, whereas a
} materials TEM would be regarded as having more capabilities. Most
} biological studies have to do with capturing images while materials
} studies have to perform analyses of the specimens as well as capture
} images.
}
} Here is a summary of the major differences in terms of capabilities:
}
} Biological TEM: basic imaging, high contrast, very low magnification
} (50x or so) with good contrast, good resolution (1 nm or so), tilting
} and possibly rotation of specimen, good vacuum, primarily tungsten
} filament but also able to use LaB6 in newer ones, generally operated
} at 60-80 kV.
}
} Materials TEM: high resolution (better than 1 nm), bright gun by means
} of LaB6 or possibly field emission, able to orientate specimen in many
} ways (tilt, rotate, tilt and rotate, eucentric), advanced analytical
} capability (specialized diffraction, X-ray, EELS) possibly with STEM
} attachment, highest and clean vacuum (turbo, ion, cryo), generally
} operated at 100 kV or higher.
}
} So, maybe a better way of characterizing the two instruments would
} be: basic imaging versus analytical TEM's, since biologists could make
} use of the analytical features.
}
} It is safe to say that biologists would probably be satisfied with
} either instrument, but materials researchers would require one with
} more advanced capability.
}
} I shall now retreat to my fortified saferoom.
}
}
}
} } What precisely is the difference between a biological TEM and a materials
} }
} TEM?
}
} } What happens if you put thin sections of a resin-embedded biological
} } specimen into a TEM
} } that is used for materials science? Is the main difference what
} } happens in terms of radiation damage to the specimen? I assume the
} } biological nature of the specimen doesn't make any difference, but
} } the thinness of the sections, and the composition of the resin, might
} } do.
} }
}
}
}

--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

==============================Original Headers==============================
9, 27 -- From colijn.1-at-osu.edu Mon Dec 14 09:28:39 2009
9, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEFScEH013160
9, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:28:38 -0600
9, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by
9, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
9, 27 -- id {01NH8S5VIPO095VSIS-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com;
9, 27 -- Mon, 14 Dec 2009 10:28:38 -0500 (EST)
9, 27 -- Received: from [164.107.96.219]
9, 27 -- (vpn-164-107-96-219.vpn.ohio-state.edu [164.107.96.219])
9, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556)
9, 27 -- with ESMTPA id {01NH8S5SC2GU96O5UL-at-ecr6.ohio-state.edu} ; Mon,
9, 27 -- 14 Dec 2009 10:28:37 -0500 (EST)
9, 27 -- Date: Mon, 14 Dec 2009 10:28:33 -0500
9, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu}
9, 27 -- Subject: Re: [Microscopy] biological TEM vs. materials TEM?
9, 27 -- In-reply-to: {200912141048.nBEAmBC4013154-at-ns.microscopy.com}
9, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu
9, 27 -- To: nizets2-at-yahoo.com, Philip.Koeck-at-ki.se
9, 27 -- Cc: microscopy-at-microscopy.com
9, 27 -- Message-id: {4B2659A1.3040604-at-osu.edu}
9, 27 -- MIME-version: 1.0
9, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
9, 27 -- Content-transfer-encoding: 7bit
9, 27 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
9, 27 -- X-Env-From: auth/colijn.1-at-osu.edu
9, 27 -- References: {200912141048.nBEAmBC4013154-at-ns.microscopy.com}
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 14 Dec 2009 09:43:19 -0600
Subject: [Microscopy] Re: biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Henk,

Flame-thrower in the closet, I'm in agreement with you.
Although I'd like to see *much* lower prices on EELS imaging systems
-- then we'll see EELS use in biology take off, especially for
immuno-TEM.

Phil

} Hi Phil and Stephane,
}
} I knew I was oversimplifying and did not mean to malign my bio
} colleagues. There is no way I could dream of doing the work they do.
} Give me my nice, robust, high-contrast, simple-to-prepare, radiation
} resistant materials samples any day!
}
} I did pass over the work the cryo-tomography people are doing. It is
} really exciting stuff. Doing low-dose, high-resolution imaging on
} low-contrast samples is not a task for the faint hearted.
}
} I should point out that the microscope companies *market* microscopes
} with different capabilities to the bio community. *Most* bio people
} don't do diffraction work and I have seen "bio" scopes without an SA
} aperture. These tend to have longer focal length (lower ultimate
} resolution) objective lenses to improve the contrast of the bio samples.
} It seems that, in general, contrast is more of an issue than
} *ultimate* resolution. Tomography is still not common in the bio (or
} materials) communities. EDX is less common in the bio community than in
} the materials, though an increasing number of bio people are now doing
} EELS and spectrum imaging. If you walk into a bio EM lab, I don't think
} you will find too many STEM capable systems and I think that STEM is a
} neglected technique by much of the bio community. The contrast
} improvement in STEM seems worthwhile, though I'd be somewhat concerned
} about the sample damage.
}
} I didn't wear my asbestos garments today, so go ahead and flame away!
}
} Cheers,
} Henk
}
} nizets2-at-yahoo.com wrote:
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } As the material scientists pointed out, a "basic" microscope (as
} } described by John) would USUALLY suffice for MOST of biological
} } research. I used to know a good scientist who made his whole career
} } with a tungsten 80kV microscope without digital camera (although he
} } needs collaborations from time to time, esp. for EMtomography work).
} }
} } BUT, as Philip said, some projects in biology require much more
} } advanced microscope capabilities and I would just add 2 options to
} } the ones described by Philip:
} } - Sample tilting (and high voltage) is required for EM tomography
} } (3D reconstruction).
} } - STEM and EDX are required for element mapping (for pathologists f.ex.).
} }
} } In summary I would say that "biological TEM" has no
} } straightforward definition, because biology is a vast field with
} } plenty of applications (\ironical mode on\ in contrast to material
} } science \ironical mode off\). Of course, if you can afford it, buy
} } a FE 300kV with double tilt, EMtomography, crystallogry, cryo, STEM
} } and EDX and it will make everyone happy!
} }
} } regards
} }
} } Stephane
} }
} }
} }
} }
} } ----- Original Message ----
} } X-from: "Philip.Koeck-at-ki.se" {Philip.Koeck-at-ki.se}
} } To: nizets2-at-yahoo.com
} } Sent: Mon, December 14, 2009 10:11:17 AM
} } Subject: [Microscopy] Re: biological TEM vs. materials TEM?
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I think you are both missing an important area of biological TEM:
} } Cryo imaging and diffraction for structural biology.
} }
} } In this field we use 120 - 300 kV, magnifications between 40000 and 100000,
} } and we reach resolution better than 0.3 nm for protein crystals and around
} } 0.5 nm for noncrystalline specimens.
} }
} } Most of the microscopes are equipped with field emission guns for the high
} } spatial coherence.
} }
} } Good contrast at low spatial frequencies is normally achieved by
} } defocussing, not by low voltage.
} }
} } Being able to cool the specimen to the temperature of liquid nitrogen
} } or even helium is one of the essential capabilities of our microscopes.
} }
} } You can come out of your fortified saferoom again. I've finished. :)
} }
} } Philip
} }
} }
} } -----Original Message-----
} } X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
} } Sent: 12 December 2009 18:08
} } To: Philip.Koeck-at-ki.se
} } Subject: [Microscopy] Re: biological TEM vs. materials TEM?
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Great question!
} }
} } The differences between a TEM used primarily for biological
} } investigations (biological TEM) and a TEM used principally for
} } materials work (materials TEM) has to do with convenience.
} }
} } Both instruments could be used to examine either type of specimen, but
} } they are configured to expedite the work in one or the other mode. For
} } example, using specialized diffraction procedures on a conventional,
} } biological TEM requires a lot of instrument tweaking (primarily
} } alignment), whereas, the same procedure could be as simple as pushing
} } a button on a materials TEM.
} }
} } At the risk of making a sweeping generalization (but probably
} } stimulating some discussion), I would say that a conventional,
} } biological TEM could be regarded as a basic instrument, whereas a
} } materials TEM would be regarded as having more capabilities. Most
} } biological studies have to do with capturing images while materials
} } studies have to perform analyses of the specimens as well as capture
} } images.
} }
} } Here is a summary of the major differences in terms of capabilities:
} }
} } Biological TEM: basic imaging, high contrast, very low magnification
} } (50x or so) with good contrast, good resolution (1 nm or so), tilting
} } and possibly rotation of specimen, good vacuum, primarily tungsten
} } filament but also able to use LaB6 in newer ones, generally operated
} } at 60-80 kV.
} }
} } Materials TEM: high resolution (better than 1 nm), bright gun by means
} } of LaB6 or possibly field emission, able to orientate specimen in many
} } ways (tilt, rotate, tilt and rotate, eucentric), advanced analytical
} } capability (specialized diffraction, X-ray, EELS) possibly with STEM
} } attachment, highest and clean vacuum (turbo, ion, cryo), generally
} } operated at 100 kV or higher.
} }
} } So, maybe a better way of characterizing the two instruments would
} } be: basic imaging versus analytical TEM's, since biologists could make
} } use of the analytical features.
} }
} } It is safe to say that biologists would probably be satisfied with
} } either instrument, but materials researchers would require one with
} } more advanced capability.
} }
} } I shall now retreat to my fortified saferoom.
} }
} }
} }
} } } What precisely is the difference between a biological TEM and a materials
} } }
} } TEM?
} }
} } } What happens if you put thin sections of a resin-embedded biological
} } } specimen into a TEM
} } } that is used for materials science? Is the main difference what
} } } happens in terms of radiation damage to the specimen? I assume the
} } } biological nature of the specimen doesn't make any difference, but
} } } the thinness of the sections, and the composition of the resin, might
} } } do.
} } }
} }
} }
} }
}
} --
} Hendrik O. Colijn
} www.ceof.ohio-state.edu
} OSU Campus Electron Optics Facility colijn.1-at-osu.edu
} 040 Fontana Labs (614) 292-0674 (V)
} 116 W. 19th Ave. (614) 292-7523 (F)
} Columbus, OH 43210
}
} "Time is that quality of nature which keeps things from happening all at
} once. Lately it doesn't seem to be working."

==============================Original Headers==============================
4, 25 -- From oshel1pe-at-cmich.edu Mon Dec 14 09:43:18 2009
4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEFhISf010103
4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:43:18 -0600
4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
4, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEFhHpB021093
4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 10:43:17 -0500
4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
4, 25 -- Mon, 14 Dec 2009 10:43:17 -0500
4, 25 -- Mime-Version: 1.0
4, 25 -- Message-Id: {f06240800c74c0cc94c98-at-[141.209.160.249]}
4, 25 -- In-Reply-To: {200912141532.nBEFWQas024207-at-ns.microscopy.com}
4, 25 -- References: {200912141532.nBEFWQas024207-at-ns.microscopy.com}
4, 25 -- Date: Mon, 14 Dec 2009 10:43:16 -0500
4, 25 -- To: Microscopy-at-microscopy.com
4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 25 -- Subject: [Microscopy] Re: biological TEM vs. materials TEM?
4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 25 -- X-OriginalArrivalTime: 14 Dec 2009 15:43:17.0693 (UTC) FILETIME=[27D916D0:01CA7CD4]
4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
4, 25 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5)
4, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
4, 25 -- X-CanItPRO-Stream: default
4, 25 -- X-Canit-Stats-ID: 24413258 - c654f8e5823d - 20091214
4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 14 Dec 2009 09:53:21 -0600
Subject: [Microscopy] Re: SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Danny,

If you're drying the bacteria on the membranes, why bother to CPD
them? They'll already have lots of drying artifacts. Which may not be
relevant, if what you're after is bacterial counts, and you can
reliably distinguish air-dried bacteria from schmutz.
Just dry, mount, sputter-coat and examine.

And: fixation is something done before drying, not after. Filter,
fix, dehydrate, then dry from 100% EtOH or from HMDS
(hexamehtyldisilizane, check the list archives, lots of posts about
that), or EtOH to CPD. Amyl acetate isn't needed. Bacteria on fliters
generally are good with 3 5-minute soaks in the CPD, after the first
flush to get rid of the bulk EtOH.

But mounting the filter membrane onto a stub before doing anything
else is putting the cart before the horse while it's still in the
stable. Putting the membrane on the stub is done just before
sputter-coating.

Phil

} Hi all,
}
} Need some advice on the best method to go with for the preparation
} of my biological samples for SEM.
}
} My samples are prepared by filtrating my bacterial suspension onto
} nucleopore track etched membrane filters. After the bacteria have
} been filtered the membranes are left on agar slabs to dry for 1 hour
} so the bacteria can be well attached.
}
} The bacteria on the membrane will be treated with cold atmospheric
} plasma to inactivate the bacteria. I need to use SEM to see how the
} bacteria are depositing on the membrane filters at my desired
} bacterial concentration.
}
} The Current preparation protocol procedure I have been using is as follows:
}
} 1) Filter bacterial suspension on to membrane and allow to
} dry/attach for 1 hour.
}
} 2) Stick the membrane filtered bacteria onto a SEM stub with a
} Carbon sticky pad.
}
} 3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.
}
} 4) Wash in distilled water then grade up to absolute ethanol 20% - 100%
}
} 5) Grade fully dehydrated sample in amyl acetate up to 100%.
}
} 6) CPD the samples using liquid CO2 passing it through its critical point.
}
} The problems I'm having with my method is the best way to secure the
} membrane down for the critical point drying because the carbon
} sticky pad attachment to the stub works fine until I grade the
} sample in Amyl acetate then the sticky pad starts to detach from the
} membrane.
}
} Is it ok to just use ethanol even though it doesn't mix as well with
} liquid CO2 and just run a longer time with more flushes in the CPD?
}
} Does anyone have any easier suggestions for securing the membrane
} down so the membrane doesn't get damaged or effected during the
} dehydration and CPD stages?
}
} Thanks
}
} Danny
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
6, 25 -- From oshel1pe-at-cmich.edu Mon Dec 14 09:53:20 2009
6, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEFrKuv024931
6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:53:20 -0600
6, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEFrEYF022926
6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 10:53:20 -0500
6, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 25 -- Mon, 14 Dec 2009 10:53:18 -0500
6, 25 -- Mime-Version: 1.0
6, 25 -- Message-Id: {f06240801c74c0da47fda-at-[141.209.160.249]}
6, 25 -- In-Reply-To: {200912141532.nBEFWQKR024212-at-ns.microscopy.com}
6, 25 -- References: {200912141532.nBEFWQKR024212-at-ns.microscopy.com}
6, 25 -- Date: Mon, 14 Dec 2009 10:53:17 -0500
6, 25 -- To: Microscopy-at-microscopy.com
6, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 25 -- Subject: Re: [Microscopy] SEM bacterial sample prep
6, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 25 -- X-OriginalArrivalTime: 14 Dec 2009 15:53:19.0006 (UTC) FILETIME=[8E422BE0:01CA7CD5]
6, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 25 -- X-Spam-Score: -4.40 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5)
6, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 25 -- X-CanItPRO-Stream: default
6, 25 -- X-Canit-Stats-ID: 24414245 - 1aa5a80cc456 - 20091214
6, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Mon, 14 Dec 2009 09:58:28 -0600
Subject: [Microscopy] Re: Should I replace retired EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I should have included this with my earlier question. We are using Kevex
Sigma Energy Dispersive X-Ray Analysis System Level KS 2 which was installed
in 1998. Can I get a supportable detector that will run with that software?
Or perhaps there is somebody out there that will continue support of the
detector.

Original Posting:
I'm seeking advice. Our service contractor just informed me that they are
retiring the EDS detector on our Cambridge Stereoscan 200 SEM (Circa 1984)
effective at the expiration of our current contract in February. They will
no longer support the detector after that time. Should I consider an upgrade
on such a vintage instrument or just continue without the service contract?

Thanks,

Jeff Stewart
Metallographic Lab Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329


==============================Original Headers==============================
5, 40 -- From SRS0=KIwxxE=IA=metallography.com=jeff-at-eigbox.net Mon Dec 14 09:58:28 2009
5, 40 -- Received: from bosmailout11.eigbox.net (bosmailout11.eigbox.net [66.96.186.11])
5, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEFwREw003337
5, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 09:58:28 -0600
5, 40 -- Received: from bosmailscan14.eigbox.net ([10.20.15.14])
5, 40 -- by bosmailout11.eigbox.net with esmtp (Exim)
5, 40 -- id 1NKDJZ-0004f0-8Z
5, 40 -- for microscopy-at-microscopy.com; Mon, 14 Dec 2009 10:58:25 -0500
5, 40 -- Received: from bosimpout02.eigbox.net ([10.20.55.2])
5, 40 -- by bosmailscan14.eigbox.net with esmtp (Exim)
5, 40 -- id 1NKDJZ-0006JK-4X
5, 40 -- for microscopy-at-microscopy.com; Mon, 14 Dec 2009 10:58:25 -0500
5, 40 -- Received: from bosauthsmtp09.eigbox.net ([10.20.18.9])
5, 40 -- by bosimpout02.eigbox.net with NO UCE
5, 40 -- id H3xu1d0010BkY8i013xuAA; Mon, 14 Dec 2009 10:57:54 -0500
5, 40 -- X-EN-OrigOutIP: 10.20.18.9
5, 40 -- X-EN-IMPSID: H3xu1d0010BkY8i013xuAA
5, 40 -- Received: from [64.17.227.194] (helo=jstewart)
5, 40 -- by bosauthsmtp09.eigbox.net with esmtpa (Exim)
5, 40 -- id 1NKDJY-0000hS-LM
5, 40 -- for Microscopy-at-microscopy.com; Mon, 14 Dec 2009 10:58:24 -0500
5, 40 -- Message-ID: {000901ca7cd7$29a56b00$461510ac-at-sternmark.local}
5, 40 -- Reply-To: "Jeff Stewart" {jeff-at-metallography.com}
5, 40 -- From: "Jeff Stewart" {jeff-at-metallography.com}
5, 40 -- To: {Microscopy-at-microscopy.com}
5, 40 -- Subject: Re: Should I replace retired EDS detector
5, 40 -- Date: Mon, 14 Dec 2009 11:04:46 -0500
5, 40 -- MIME-Version: 1.0
5, 40 -- Content-Type: text/plain;
5, 40 -- charset="iso-8859-1"
5, 40 -- Content-Transfer-Encoding: 7bit
5, 40 -- X-Priority: 3
5, 40 -- X-MSMail-Priority: Normal
5, 40 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1106
5, 40 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1106
5, 40 -- X-EN-UserInfo: e86f17d72db2b073c5072c937c9a2a3f:f6ee1f837e2a1583e399b28f5cf1cc41
5, 40 -- X-EN-AuthUser: jeff-at-metallography.com
5, 40 -- Sender: "Jeff Stewart" {jeff-at-metallography.com}
5, 40 -- X-EN-OrigIP: 64.17.227.194
5, 40 -- X-EN-OrigHost: unknown
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 14 Dec 2009 10:51:53 -0600
Subject: [Microscopy] SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Danny,

Ah, I understand. Still, you can't mount the membranes to stubs
before they are dry.

So, for this situation, put the membranes in small petri dishes or a
multi-well plate big enough for the membrane to lie flat, then flood
with glut. Fix, then remove and flood with EtOH. Do this for your
dehydration series. After the final 100% EtOH, do a 1:1 EtOH:HMDS
then 3 X 100% HMDS and air dry. The HMDS **MUST** be used in a fume
hood. Mount the dried membranes.
Note: I routinely run unsupported membranes through CPDs without loss
or problems, but your particular situation may indeed require more
careful handling, therefore HMDS drying.

Just be careful adding and removing the solutions -- I'd do that with
2 pipettes, one to withdraw while adding new solution with the other,
both of them off to the side so they're not squirting/sucking
directly over the membrane.

Phil

} Hi Phil,
}
} I understand about drying being after fixation and dehydration.
} Drying was probaly the wrong word to use because once the bacteria
} have been filtered onto the membranes they are transfered to agar
} slabs which are moist and keep the sample fairly wet so the hour on
} the agar slabs is mainly for the bacteria to settle and attach
} before I treat them so they don't just blow off in the gas flow.
}
} I don't want to count the bacteria with the SEM I just want to
} visually see how they are being deposited e.g stacking or a
} monolayer with minimal amounts of stacking, this is due to the
} treatment I'm using shows potential protection when the bacteria are
} more stacked.
}
} The reason why I mounted the membrane on the stub was to support the
} membrane during the fixing and dryig to minimise damage by
} constantly touching it with tweezers when grading through the
} alcohol. I also don't want the membrane flipping over so I lose
} which side I deposited them on and damaging the bacteria during the
} CPD.
}
} The CPD I have is a E3100 Quroum technologies which is a horizontal
} one and comes with boats to hold the samples but my membranes are
} too big to fit the designed boats so I need some way of securing the
} membranes minimising any risk of damage.
}
} Thanks
}
} Danny
}
}
} On Mon, 14 Dec 2009 10:00:39 -0600
} oshel1pe-at-cmich.edu wrote:
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} } Danny,
} } If you're drying the bacteria on the membranes, why bother to CPD
} } them? They'll already have lots of drying artifacts. Which may not
} } be relevant, if what you're after is bacterial counts, and you can
} } reliably distinguish air-dried bacteria from schmutz.
} } Just dry, mount, sputter-coat and examine.
} } And: fixation is something done before drying, not after. Filter,
} } fix, dehydrate, then dry from 100% EtOH or from HMDS
} } (hexamehtyldisilizane, check the list archives, lots of posts about
} } that), or EtOH to CPD. Amyl acetate isn't needed. Bacteria on
} } fliters generally are good with 3 5-minute soaks in the CPD, after
} } the first flush to get rid of the bulk EtOH.
} } But mounting the filter membrane onto a stub before doing anything
} } else is putting the cart before the horse while it's still in the
} } stable. Putting the membrane on the stub is done just before
} } sputter-coating.
} } Phil
} }
} } } Hi all,
} } }
} } } Need some advice on the best method to go with for the preparation
} } } of my biological samples for SEM.
} } }
} } } My samples are prepared by filtrating my bacterial suspension onto
} } } nucleopore track etched membrane filters. After the bacteria have
} } } been filtered the membranes are left on agar slabs to dry for 1
} } } hour so the bacteria can be well attached.
} } }
} } } The bacteria on the membrane will be treated with cold atmospheric
} } } plasma to inactivate the bacteria. I need to use SEM to see how
} } } the bacteria are depositing on the membrane filters at my desired
} } } bacterial concentration.
} } }
} } } The Current preparation protocol procedure I have been using is as follows:
} } }
} } } 1) Filter bacterial suspension on to membrane and allow to
} } } dry/attach for 1 hour.
} } }
} } } 2) Stick the membrane filtered bacteria onto a SEM stub with a
} } } Carbon sticky pad.
} } }
} } } 3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.
} } }
} } } 4) Wash in distilled water then grade up to absolute ethanol 20% - 100%
} } }
} } } 5) Grade fully dehydrated sample in amyl acetate up to 100%.
} } }
} } } 6) CPD the samples using liquid CO2 passing it through its critical point.
} } }
} } } The problems I'm having with my method is the best way to secure
} } } the membrane down for the critical point drying because the carbon
} } } sticky pad attachment to the stub works fine until I grade the
} } } sample in Amyl acetate then the sticky pad starts to detach from
} } } the membrane.
} } }
} } } Is it ok to just use ethanol even though it doesn't mix as well
} } } with liquid CO2 and just run a longer time with more flushes in
} } } the CPD?
} } }
} } } Does anyone have any easier suggestions for securing the membrane
} } } down so the membrane doesn't get damaged or effected during the
} } } dehydration and CPD stages?
} } }
} } } Thanks
} } }
} } } Danny
} } -- Philip Oshel
} } Microscopy Facility Supervisor
} } Biology Department
} } 024C Brooks Hall
} } Central Michigan University
} } Mt. Pleasant, MI 48859
} } (989) 774-3576

==============================Original Headers==============================
6, 27 -- From oshel1pe-at-cmich.edu Mon Dec 14 10:51:51 2009
6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEGpo6Q025632
6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 10:51:51 -0600
6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEGoXmR031860;
6, 27 -- Mon, 14 Dec 2009 11:51:23 -0500
6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 -- Mon, 14 Dec 2009 11:51:21 -0500
6, 27 -- Mime-Version: 1.0
6, 27 -- Message-Id: {f06240803c74c1bacc9d2-at-[141.209.160.249]}
6, 27 -- In-Reply-To: {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- References: {200912141600.nBEG0dZw010972-at-ns.microscopy.com}
6, 27 -- {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- Date: Mon, 14 Dec 2009 11:51:18 -0500
6, 27 -- To: D.L.Bayliss-at-lboro.ac.uk
6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 27 -- Subject: Re: [Microscopy] Re: SEM bacterial sample prep
6, 27 -- Cc: Microscopy-at-microscopy.com
6, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 27 -- X-OriginalArrivalTime: 14 Dec 2009 16:51:21.0943 (UTC) FILETIME=[AA402E70:01CA7CDD]
6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 27 -- X-Spam-Score: -3.40 () [Hold at 6.00] L_EXCH_MF,L_USD,PORN_RP_SUCKING,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 27 -- X-CanItPRO-Stream: default
6, 27 -- X-Canit-Stats-ID: 24418988 - 049e1da62fd1 - 20091214
6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================




From: connellyps-at-nhlbi.nih.gov
Date: Mon, 14 Dec 2009 13:35:53 -0600
Subject: [Microscopy] SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Danny,

I agree with Phil and would like to add the following suggestion:

If you do need to use the CPD to see detail...

We use filters that fit into the CPD holder for cover-slips. After fixations (2.5% glutaraldehyde, buffer wash, 1% Osmium tetroxide) wash with water then under water several filters are loaded into the holder with the wavy washers under and between each filter, a final one on top then cap off with the weight. Ethanol dehydration 15%, 30%, 50%, 70%, 80%, 90% then a few changes of 100% from a freshly opened bottle and a sieve dried 100% ethanol change. This dried ethanol is also what we use to fill the CPD chamber. The ethanol steps are carried out in 100 ml tri-pour beakers that are fitted with a stir bar and a "U"-shaped piece of screening above it to support the holder, 10 to 15 min. each exchange works well for fine detail like cilia and microvilli etc. When the beakers are filled to a level above the samples and put onto a stirring apparatus the ethanol freely exchanges. In using two beakers we can quickly transfer the holder easily from one to the other (without draining) then change the ethanol for the next exchange just before needing it.

The above method also works for cell culture insert membranes (the size that fits into 12 well plates) that are cut out of the supporting plastic in the water step after fixation.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E - Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-480-6560
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.

==
Danny,

If you're drying the bacteria on the membranes, why bother to CPD
them? They'll already have lots of drying artifacts. Which may not be
relevant, if what you're after is bacterial counts, and you can
reliably distinguish air-dried bacteria from schmutz.
Just dry, mount, sputter-coat and examine.

And: fixation is something done before drying, not after. Filter,
fix, dehydrate, then dry from 100% EtOH or from HMDS
(hexamehtyldisilizane, check the list archives, lots of posts about
that), or EtOH to CPD. Amyl acetate isn't needed. Bacteria on fliters
generally are good with 3 5-minute soaks in the CPD, after the first
flush to get rid of the bulk EtOH.

But mounting the filter membrane onto a stub before doing anything
else is putting the cart before the horse while it's still in the
stable. Putting the membrane on the stub is done just before
sputter-coating.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

} Hi all,
}
} Need some advice on the best method to go with for the preparation
} of my biological samples for SEM.
}
} My samples are prepared by filtrating my bacterial suspension onto
} nucleopore track etched membrane filters. After the bacteria have
} been filtered the membranes are left on agar slabs to dry for 1 hour
} so the bacteria can be well attached.
}
} The bacteria on the membrane will be treated with cold atmospheric
} plasma to inactivate the bacteria. I need to use SEM to see how the
} bacteria are depositing on the membrane filters at my desired
} bacterial concentration.
}
} The Current preparation protocol procedure I have been using is as follows:
}
} 1) Filter bacterial suspension on to membrane and allow to
} dry/attach for 1 hour.
}
} 2) Stick the membrane filtered bacteria onto a SEM stub with a
} Carbon sticky pad.
}
} 3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.
}
} 4) Wash in distilled water then grade up to absolute ethanol 20% - 100%
}
} 5) Grade fully dehydrated sample in amyl acetate up to 100%.
}
} 6) CPD the samples using liquid CO2 passing it through its critical point.
}
} The problems I'm having with my method is the best way to secure the
} membrane down for the critical point drying because the carbon
} sticky pad attachment to the stub works fine until I grade the
} sample in Amyl acetate then the sticky pad starts to detach from the
} membrane.
}
} Is it ok to just use ethanol even though it doesn't mix as well with
} liquid CO2 and just run a longer time with more flushes in the CPD?
}
} Does anyone have any easier suggestions for securing the membrane
} down so the membrane doesn't get damaged or effected during the
} dehydration and CPD stages?
}
} Thanks
}
} Danny




==============================Original Headers==============================
18, 31 -- From connellyps-at-nhlbi.nih.gov Mon Dec 14 13:35:53 2009
18, 31 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112])
18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEJZqgO028650
18, 31 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 13:35:52 -0600
18, 31 -- X-IronPortListener: Outbound_SMTP
18, 31 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117])
18, 31 -- by nihxway4out.hub.nih.gov with ESMTP; 14 Dec 2009 14:35:19 -0500
18, 31 -- Received: from NIHHT03.nih.gov ([156.40.71.22]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959);
18, 31 -- Mon, 14 Dec 2009 14:35:17 -0500
18, 31 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT03.nih.gov
18, 31 -- ([156.40.71.22]) with mapi; Mon, 14 Dec 2009 14:35:17 -0500
18, 31 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov}
18, 31 -- To: Phil Oshel {oshel1pe-at-cmich.edu} ,
18, 31 -- "microscopy-at-microscopy.com"
18, 31 -- {microscopy-at-microscopy.com}
18, 31 -- Date: Mon, 14 Dec 2009 14:34:51 -0500
18, 31 -- Subject: Re: [Microscopy] Re: SEM bacterial sample prep
18, 31 -- Thread-Topic: [Microscopy] Re: SEM bacterial sample prep
18, 31 -- Thread-Index: Acp81zdevwU8yaxrSxO+GrSp4yBNNgAHUmLa
18, 31 -- Message-ID: {C74BFD8B.45D1%connellyps-at-nhlbi.nih.gov}
18, 31 -- In-Reply-To: {200912141556.nBEFuOY6032022-at-ns.microscopy.com}
18, 31 -- Accept-Language: en-US
18, 31 -- Content-Language: en
18, 31 -- X-MS-Has-Attach:
18, 31 -- X-MS-TNEF-Correlator:
18, 31 -- acceptlanguage: en-US
18, 31 -- Content-Type: text/plain; charset="iso-8859-1"
18, 31 -- MIME-Version: 1.0
18, 31 -- X-OriginalArrivalTime: 14 Dec 2009 19:35:17.0955 (UTC) FILETIME=[90F88130:01CA7CF4]
18, 31 -- Content-Transfer-Encoding: 8bit
18, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBEJZqgO028650
==============================End of - Headers==============================




From: naomi_mccallum-at-health.qld.gov.au
Date: Mon, 14 Dec 2009 20:44:17 -0600
Subject: [Microscopy] Fwd: viaWWW: Question about Image Database Programs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert

We are a diagnostic pathology laboratory and hence have the need to store images (and associated files) in a secure searchable database. We started using STREAM (Olympus Soft Imaging Solutions) over a year ago after previously using the DB in iTEM (also from Olympus-SIS) and are quite happy with it so far. We have it set up on a SQL base but I think you can use Oracle as well.

The selling features for us were the security with the ability to configure groups of users with corresponding permission sets to tailor who has access to what. And also the compatibility with our camera systems. For LM we can acquire images using the software and save them straight to the DB. For EM we still prefer to use iTEM but there is a window interface to the Stream DB which makes transfer of file quick & easy. I understand that the latest version has incorporated drivers for more cameras allowing them direct acquisition.

The software allows application of comprehensive filters and searches and those used frequently can be saved. These functions work well but are not as pretty (some say user-friendly) wrt the layout of the window which is something we have discussed with O-SIS.

I understand that this system is in use in a number of diagnostic and research facilities in Australia.

Disclaimer: Of course this HO must be tempered by the fact that I have not trialed any other system currently available. No kickbacks received, satisfied customer.

Naomi
EM Unit - Pathology Queensland
Royal Brisbane & Women's Hospital
Australia


} } } {ropope-at-gmail.com} 15/12/2009 12:43 am } } }



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ropope-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ropope-at-gmail.com
Name: Robert Pope

Organization: BNBI

Title-Subject: [Filtered] Question about Image Database Programs

Question: Good Day Everyone:
I have monitored the microscopy listserve for many years and only
reply to threads when I think I have meaningful insight. I have a
question for all the experts in the field. I have developed image
archives in the past, but would like to set up a new image database
that is searchable. I have been working with FileMaker Pro and have
had good results, but wanted to see if any of you have had luck with
other programs that are off the shelf. I realize building your own
database and writing code tends to work best, but I need to use an
off the shelf product for this specific project. If you have any
meaningful comments, I would be most grateful to read them.
Thank you,
Robert

Robert K. Pope, Ph.D.
Principal Investigator
Electron Microscopy Laboratory Manager
ropope-at-gmail.com

I remember when green screen was a circle of beautiful special
effects in the TEM (seeing what no one has seen before), and not a
mechanism for special effects in the theater.


Login Host: 12.198.101.3
---------------------------------------------------------------------------

==============================Original Headers==============================
9, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 08:34:03 2009
9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEEY0j1032519
9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 08:34:02 -0600
9, 11 -- Mime-Version: 1.0
9, 11 -- Message-Id: {p06240800c74bfd45a785-at-[206.69.208.22]}
9, 11 -- Date: Mon, 14 Dec 2009 08:34:00 -0600
9, 11 -- To: microscopy-at-microscopy.com
9, 11 -- From: ropope-at-gmail.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: Question about Image Database Programs
9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

********************************************************************************
This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.
**********************************************************************************




==============================Original Headers==============================
3, 25 -- From prvs=1600037094=naomi_mccallum-at-health.qld.gov.au Mon Dec 14 20:44:17 2009
3, 25 -- Received: from gwd-mailedge04.health.qld.gov.au (smtp2.health.qld.gov.au [165.86.81.113])
3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF2iFLI027364
3, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 20:44:16 -0600
3, 25 -- Received: from gwd-mail04.remote.health.qld.gov.au (gwd-mail04.remote.health.qld.gov.au [192.168.3.51])
3, 25 -- by gwd-mailedge04.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id nBF2hqCC005401
3, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 12:43:53 +1000
3, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by
3, 25 -- gwd-mail04.remote.health.qld.gov.au (Queensland Health SMTP Server)
3, 25 -- with ESMTP id
3, 25 -- {T929ec67a3bc0a8033318e0-at-gwd-mail04.remote.health.qld.gov.au} for
3, 25 -- {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 12:43:52 +1000
3, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with
3, 25 -- Novell_GroupWise; Tue, 15 Dec 2009 12:43:52 +1000
3, 25 -- Message-Id: {4B27847A.88BE.00AA.0-at-health.qld.gov.au}
3, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3
3, 25 -- Date: Tue, 15 Dec 2009 12:43:40 +1000
3, 25 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au}
3, 25 -- To: {ropope-at-gmail.com} , {Microscopy-at-microscopy.com}
3, 25 -- Subject: Fwd: [Microscopy] viaWWW: Question about Image Database Programs
3, 25 -- Mime-Version: 1.0
3, 25 -- Content-Type: text/plain; charset="us-ascii"
3, 25 -- Content-Disposition: inline
3, 25 -- Content-Transfer-Encoding: 8bit
3, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBF2iFLI027364
==============================End of - Headers==============================




From: rajeevrajvihar-at-gmail.com
Date: Mon, 14 Dec 2009 21:15:07 -0600
Subject: [Microscopy] viaWWW: Particle size and laser wavelength

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rajeevrajvihar-at-gmail.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rajeevrajvihar-at-gmail.com
Name: Rajeev

Organization: Indian Space Research Organization

Title-Subject: [Filtered] Particle size and laser wavelength

Question: I am sorry if this question not exactly fits to Microscopy
forum. My question is regarding particle size measurement using laser
diffraction technique and dynamic light scattering. What is the
relationship between wavelength of the laser beam and the size of
particle that can be measured using it. There is a belief that the
limit in such measurements is the wavelength of the laser beam so
that a 633 nm laser beam cannot measure accurately the sizes lower
than 633 nm.

Is it correct that the lower limit of particle size measurement using
laser techniques is the wavelength of the laser beam? In such cases,
how nanoparticle size analyzers measure particle size in the range 1
nm - 100 nm?

Thanks in advance. Sorry if the question is misplaced.

Regards,

Dr. R.S. Rajeev
Indian Space Research Organization
India.

Login Host: 117.199.10.215
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 21:15:06 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF3F5c4010182
10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 21:15:06 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240801c74caf876fd4-at-[206.69.208.22]}
10, 11 -- Date: Mon, 14 Dec 2009 21:15:04 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: rajeevrajvihar-at-gmail.com (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Particle size and laser wavelength
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: l-reyjr-at-northwestern.edu
Date: Mon, 14 Dec 2009 21:15:43 -0600
Subject: [Microscopy] viaWWW: Pelleting Cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both l-reyjr-at-northwestern.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: Northwestern University

Title-Subject: [Filtered] Pelleting Cells

Question: I need to pellet some neutrophils. What is the recommended
rpm and time? How should I dislodge pellet from the side of the
ependorff tube?

Many Thanks
Lennell

Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 21:15:43 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF3FgwU011268
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 21:15:42 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c74cafc47e00-at-[206.69.208.22]}
7, 11 -- Date: Mon, 14 Dec 2009 21:15:41 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Pelleting Cells
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mbisher-at-princeton.edu
Date: Mon, 14 Dec 2009 21:16:27 -0600
Subject: [Microscopy] viaWWW: Hydrophobic Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] Hydrophobic Grids

Question: I have a collaborator here who wants to do negative
staining of proteins on a lipid surface. She needs to have the lipid
surface in contact with the carbon film on the grid. So this means
she needs to have the carbon film be hydrophobic.
I know how to make them hydrophilic by glow discharge, what's the way
to make them hydrophobic?
Any sugggestions? Thank you.

Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher-at-princeton.edu

Login Host: 128.112.160.214
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 21:16:27 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF3GOl0013532
7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 21:16:26 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240803c74cafee87ee-at-[206.69.208.22]}
7, 11 -- Date: Mon, 14 Dec 2009 21:16:24 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Hydrophobic Grids
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Tue, 15 Dec 2009 05:34:42 -0600
Subject: [Microscopy] Re: viaWWW: Hydrophobic Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Margaret,

The nature of the charge on the carbon can be controlled by the
composition of the residual gas/vapor in the plasma coating unit.
Harrick has some information on their website in the product description:

Surface Activation and Modification:
http://www.harrickplasma.com/applications_activation.php

Surface Adhesion and Wettability:
http://www.harrickplasma.com/applications_adhesion.php

Many units intended for plasma treatment, including the Harrick, have a
fitting for admitting a metered amount of the chosen material.

Disclaimer: I have no connection to Harrick Plasma other than being a
happy user of their product.

Dale

Dale Callaham
The University of Massachusetts -at- Amherst


mbisher-at-princeton.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: mbisher-at-princeton.edu
} Name: Margaret Bisher
}
} Organization: Princeton University
}
} Title-Subject: [Filtered] Hydrophobic Grids
}
} Question: I have a collaborator here who wants to do negative
} staining of proteins on a lipid surface. She needs to have the lipid
} surface in contact with the carbon film on the grid. So this means
} she needs to have the carbon film be hydrophobic.
} I know how to make them hydrophilic by glow discharge, what's the way
} to make them hydrophobic?
} Any sugggestions? Thank you.
}
} Margaret E. Bisher
} Electron Microscopy& Histology Core Facility Manager
} Department of Molecular Biology
} Princeton University
} Moffett Laboratory, Room 113
} Princeton, New Jersey
} Office: (609) 258-7026
} Fax: (609) 258-8468
} mbisher-at-princeton.edu
}
} Login Host: 128.112.160.214
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 21:16:27 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF3GOl0013532
} 7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 21:16:26 -0600
} 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240803c74cafee87ee-at-[206.69.208.22]}
} 7, 11 -- Date: Mon, 14 Dec 2009 21:16:24 -0600
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Hydrophobic Grids
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================

==============================Original Headers==============================
10, 20 -- From dac-at-research.umass.edu Tue Dec 15 05:34:42 2009
10, 20 -- Received: from race-4.oit.umass.edu (race-4.oit.umass.edu [128.119.2.115])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFBYfLc004932
10, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 05:34:42 -0600
10, 20 -- Received: from [192.168.1.101] (97-80-112-42.dhcp.oxfr.ma.charter.com [97.80.112.42])
10, 20 -- (authenticated bits=0)
10, 20 -- by race-4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id nBFBYeNc017126
10, 20 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT);
10, 20 -- Tue, 15 Dec 2009 06:34:41 -0500
10, 20 -- Message-ID: {4B2774A7.2050103-at-research.umass.edu}
10, 20 -- Date: Tue, 15 Dec 2009 06:36:07 -0500
10, 20 -- From: Dale Callaham {dac-at-research.umass.edu}
10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.1.4) Gecko/20091017 SeaMonkey/2.0
10, 20 -- MIME-Version: 1.0
10, 20 -- To: mbisher-at-princeton.edu, Microscopy-at-microscopy.com
10, 20 -- Subject: Re: [Microscopy] viaWWW: Hydrophobic Grids
10, 20 -- References: {200912150321.nBF3Lqe4002296-at-ns.microscopy.com}
10, 20 -- In-Reply-To: {200912150321.nBF3Lqe4002296-at-ns.microscopy.com}
10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Tue, 15 Dec 2009 05:36:18 -0600
Subject: [Microscopy] Re: viaWWW: Hydrophobic Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
There is an article from J. Dubochet in the book "Advances in
Optical and Electron Microscopy, vol. 8" from 1982. You can find there several
tips how to make carbon film hydrophilic or hydrophobic. I am sorry, I do have
the book at hand to send you more details.

Full citation:
Dubochet, J., Groom, M. & Mueller-Neuteboom, S. The mounting of
macromolecules for electron microscopy with particular reference to surface
phenomena and treatment of support films by glow discharge. In Advances in
Optical and Electron Microscopy (eds. Barrer, R. & Cosslett, V.E.) 107–135
(Academic Press, London, New York, 1982).

Best regards from Prague
Oldrich

---------------------------------------------------
Oldrich Benada
Institute of Microbiology, Acad. Sci. CR
Videnska 1083
142 20 Prague 4
Czech Republic


On Tuesday 15 of December 2009 04:19:09 mbisher-at-princeton.edu wrote:
} ---------------------------------------------------------------------------
} - The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
} --
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying please copy both mbisher-at-princeton.edu as well as the
} MIcroscopy Listserver
} --------------------------------------------------------------------------
} -
}
} Email: mbisher-at-princeton.edu
} Name: Margaret Bisher
}
} Organization: Princeton University
}
} Title-Subject: [Filtered] Hydrophobic Grids
}
} Question: I have a collaborator here who wants to do negative
} staining of proteins on a lipid surface. She needs to have the lipid
} surface in contact with the carbon film on the grid. So this means
} she needs to have the carbon film be hydrophobic.
} I know how to make them hydrophilic by glow discharge, what's the way
} to make them hydrophobic?
} Any sugggestions? Thank you.
}
} Margaret E. Bisher
} Electron Microscopy & Histology Core Facility Manager
} Department of Molecular Biology
} Princeton University
} Moffett Laboratory, Room 113
} Princeton, New Jersey
} Office: (609) 258-7026
} Fax: (609) 258-8468
} mbisher-at-princeton.edu
}
} Login Host: 128.112.160.214
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers============================== 7, 11 -- From zaluzec-at-microscopy.com
} Mon Dec 14 21:16:27 2009
} 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nBF3GOl0013532 7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009
} 21:16:26 -0600 7, 11 -- Mime-Version: 1.0
} 7, 11 -- Message-Id: {p06240803c74cafee87ee-at-[206.69.208.22]}
} 7, 11 -- Date: Mon, 14 Dec 2009 21:16:24 -0600
} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: mbisher-at-princeton.edu (by way of MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Hydrophobic Grids
} 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
6, 24 -- From benada-at-biomed.cas.cz Tue Dec 15 05:36:18 2009
6, 24 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFBaH3i006471
6, 24 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 05:36:18 -0600
6, 24 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171])
6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
6, 24 -- (No client certificate requested)
6, 24 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id E88361A445EB;
6, 24 -- Tue, 15 Dec 2009 12:35:11 +0100 (CET)
6, 24 -- From: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz}
6, 24 -- Organization: =?utf-8?q?Mikrobiogick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i.
6, 24 -- To: mbisher-at-princeton.edu
6, 24 -- Subject: Re: [Microscopy] viaWWW: Hydrophobic Grids
6, 24 -- Date: Tue, 15 Dec 2009 12:36:14 +0100
6, 24 -- User-Agent: KMail/1.12.2 (Linux/2.6.30-2-686; KDE/4.3.2; i686; ; )
6, 24 -- References: {200912150319.nBF3J90H023798-at-ns.microscopy.com}
6, 24 -- In-Reply-To: {200912150319.nBF3J90H023798-at-ns.microscopy.com}
6, 24 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com}
6, 24 -- MIME-Version: 1.0
6, 24 -- Content-Type: Text/Plain;
6, 24 -- charset="utf-8"
6, 24 -- Message-Id: {200912151236.14779.benada-at-biomed.cas.cz}
6, 24 -- Content-Transfer-Encoding: 8bit
6, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFBaH3i006471
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 15 Dec 2009 07:57:21 -0600
Subject: [Microscopy] SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Danny, I would also go the Phil's way, but in all cases it will be hard to interpret the data since you cannot exclude that post-filtration treatment (fixation, dehydration...) will wash out some cells. To avoid the possibility to wash out the cells during the treatments, I would suggest that you filter all solutions in the direction you used to filter the bacteria. You can use a filter holder (I don't remember who sell it, I think it is SPI but I may be wrong). These holders adapt to syringes. The method works really well, although the rod-shaped bacteria tend to present some artifacts while the coccus are perfectly round (sorry I am not familiar with the english terms, these are the round one). Alternatively, you can cut out a round opening in the cap of an eppendorf tube (1,5 ml), pinch a membrane between tube and cap and incubate the solutions onto the membrane. Then you can simply centrifuge to get rid of the solution, with a fine control of
the centrifuge speed (to avoid complete drying out).
These methods present the advantage of involving no manipulation of the membrane directly and keeping all the bacteria on the filter at all stages.
The last step consists of drying the membrane under the hood (HMDS), then glueing and coating.

Alternatively, you may keep the bacteria wet and use an environmental SEM (just fix them to avoid security problems with pathogens).

Best regards and happy SEMing

Stephane


----- Original Message ----
X-from: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu}
To: nizets2-at-yahoo.com
Sent: Mon, December 14, 2009 5:57:26 PM

Danny,

Ah, I understand. Still, you can't mount the membranes to stubs
before they are dry.

So, for this situation, put the membranes in small petri dishes or a
multi-well plate big enough for the membrane to lie flat, then flood
with glut. Fix, then remove and flood with EtOH. Do this for your
dehydration series. After the final 100% EtOH, do a 1:1 EtOH:HMDS
then 3 X 100% HMDS and air dry. The HMDS **MUST** be used in a fume
hood. Mount the dried membranes.
Note: I routinely run unsupported membranes through CPDs without loss
or problems, but your particular situation may indeed require more
careful handling, therefore HMDS drying.

Just be careful adding and removing the solutions -- I'd do that with
2 pipettes, one to withdraw while adding new solution with the other,
both of them off to the side so they're not squirting/sucking
directly over the membrane.

Phil

} Hi Phil,
}
} I understand about drying being after fixation and dehydration.
} Drying was probaly the wrong word to use because once the bacteria
} have been filtered onto the membranes they are transfered to agar
} slabs which are moist and keep the sample fairly wet so the hour on
} the agar slabs is mainly for the bacteria to settle and attach
} before I treat them so they don't just blow off in the gas flow.
}
} I don't want to count the bacteria with the SEM I just want to
} visually see how they are being deposited e.g stacking or a
} monolayer with minimal amounts of stacking, this is due to the
} treatment I'm using shows potential protection when the bacteria are
} more stacked.
}
} The reason why I mounted the membrane on the stub was to support the
} membrane during the fixing and dryig to minimise damage by
} constantly touching it with tweezers when grading through the
} alcohol. I also don't want the membrane flipping over so I lose
} which side I deposited them on and damaging the bacteria during the
} CPD.
}
} The CPD I have is a E3100 Quroum technologies which is a horizontal
} one and comes with boats to hold the samples but my membranes are
} too big to fit the designed boats so I need some way of securing the
} membranes minimising any risk of damage.
}
} Thanks
}
} Danny
}
}
} On Mon, 14 Dec 2009 10:00:39 -0600
}   oshel1pe-at-cmich.edu wrote:
} }
} }   ----------------------------------------------------------------------------
} }   The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} }   To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} }   On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }   ----------------------------------------------------------------------------
} }   Danny,
} }   If you're drying the bacteria on the membranes, why bother to CPD
} } them? They'll already have lots of drying artifacts. Which may not
} } be relevant, if what you're after is bacterial counts, and you can
} } reliably distinguish air-dried bacteria from schmutz.
} }   Just dry, mount, sputter-coat and examine.
} }   And: fixation is something done before drying, not after. Filter,
} } fix, dehydrate, then dry from 100% EtOH or from HMDS
} } (hexamehtyldisilizane, check the list archives, lots of posts about
} } that), or EtOH to CPD. Amyl acetate isn't needed. Bacteria on
} } fliters generally are good with 3 5-minute soaks in the CPD, after
} } the first flush to get rid of the bulk EtOH.
} }   But mounting the filter membrane onto a stub before doing anything
} } else is putting the cart before the horse while it's still in the
} } stable. Putting the membrane on the stub is done just before
} } sputter-coating.
} }   Phil
} }
} } } Hi all,
} } }
} } } Need some advice on the best method to go with for the preparation
} } } of my biological samples for SEM.
} } }
} } } My samples are prepared by filtrating my bacterial suspension onto
} } } nucleopore track etched membrane filters. After the bacteria have
} } } been filtered the membranes are left on agar slabs to dry for 1
} } } hour so the bacteria can be well attached.
} } }
} } } The bacteria on the membrane will be treated with cold atmospheric
} } } plasma to inactivate the bacteria. I need to use SEM to see how
} } } the bacteria are depositing on the membrane filters at my desired
} } } bacterial concentration.
} } }
} } } The Current preparation protocol procedure I have been using is as follows:
} } }
} } } 1) Filter bacterial suspension on to membrane and allow to
} } } dry/attach for 1 hour.
} } }
} } } 2) Stick the membrane filtered bacteria onto a SEM stub with a
} } } Carbon sticky pad.
} } }
} } } 3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.
} } }
} } } 4) Wash in distilled water then grade up to absolute ethanol 20% - 100%
} } }
} } } 5) Grade fully dehydrated sample in amyl acetate up to 100%.
} } }
} } } 6) CPD the samples using liquid CO2 passing it through its critical point.
} } }
} } } The problems I'm having with my method is the best way to secure
} } } the membrane down for the critical point drying because the carbon
} } } sticky pad attachment to the stub works fine until I grade the
} } } sample in Amyl acetate then the sticky pad starts to detach from
} } } the membrane.
} } }
} } } Is it ok to just use ethanol even though it doesn't mix as well
} } } with liquid CO2 and just run a longer time with more flushes in
} } } the CPD?
} } }
} } } Does anyone have any easier suggestions for securing the membrane
} } } down so the membrane doesn't get damaged or effected during the
} } } dehydration and CPD stages?
} } }
} } } Thanks
} } }
} } } Danny
} }   -- Philip Oshel
} }   Microscopy Facility Supervisor
} }   Biology Department
} }   024C Brooks Hall
} }   Central Michigan University
} }   Mt. Pleasant, MI 48859
} }   (989) 774-3576

==============================Original Headers==============================
6, 27 -- From oshel1pe-at-cmich.edu Mon Dec 14 10:51:51 2009
6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEGpo6Q025632
6, 27 --     for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 10:51:51 -0600
6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 27 --     by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEGoXmR031860;
6, 27 --     Mon, 14 Dec 2009 11:51:23 -0500
6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 --     Mon, 14 Dec 2009 11:51:21 -0500
6, 27 -- Mime-Version: 1.0
6, 27 -- Message-Id: {f06240803c74c1bacc9d2-at-[141.209.160.249]}
6, 27 -- In-Reply-To: {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- References: {200912141600.nBEG0dZw010972-at-ns.microscopy.com}
6, 27 --  {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- Date: Mon, 14 Dec 2009 11:51:18 -0500
6, 27 -- To: D.L.Bayliss-at-lboro.ac.uk
6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 27 -- Subject: Re: [Microscopy] Re: SEM bacterial sample prep
6, 27 -- Cc: Microscopy-at-microscopy.com
6, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 27 -- X-OriginalArrivalTime: 14 Dec 2009 16:51:21.0943 (UTC) FILETIME=[AA402E70:01CA7CDD]
6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 27 -- X-Spam-Score: -3.40 () [Hold at 6.00] L_EXCH_MF,L_USD,PORN_RP_SUCKING,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 27 -- X-CanItPRO-Stream: default
6, 27 -- X-Canit-Stats-ID: 24418988 - 049e1da62fd1 - 20091214
6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================






==============================Original Headers==============================
21, 24 -- From nizets2-at-yahoo.com Tue Dec 15 07:57:21 2009
21, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBFDvKna006546
21, 24 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 07:57:21 -0600
21, 24 -- Received: (qmail 42297 invoked by uid 60001); 15 Dec 2009 13:57:20 -0000
21, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1260885440; bh=b17FVeZnFaKCECNwLj9VvGqb72rpM9ONPSNmhux9ApM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=n5j7xsaqSvbFZr0zgllDSe2lMrrGrSvKrfUGEBternoUNmt3lw8ZvvxobsosXVcQrf/KWIhAPDQ3USTYlb2T2E6uMy3taTzCzmVQC6FeE/QwpViqt71RN2qlqc8k8wO1KTMNv3vnBQ0cgQou0vRSGgz7hj7s0Nha0g/RNm05/M8=
21, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
21, 24 -- s=s1024; d=yahoo.com;
21, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
21, 24 -- b=xmoR3SA35IiuvF7jwB2erksAZ+u3UxBw2E7u+bJok+/QuaC4lcBPraAu0hBg9be7HkTw520kt5W74ogPrAKDfFlA1+ZsziB0UBbWYAG8/A7S5xbuxLXxvKnQJx55dP4fDG2/0g1TC11juzNBQ5pPG44hBQw8z50ByoTvDVkR/wk=;
21, 24 -- Message-ID: {408386.41792.qm-at-web110801.mail.gq1.yahoo.com}
21, 24 -- X-YMail-OSG: i2VzE4QVM1mvJqFDaU617qnOSonhA1Yqa4hlQuFJYNBpR4hFI52FJFcsoxP0OPWRGNYhQaRzrGqUf3FVkTrsvTGb9f27OVhKr3MdEtmK5caQXy1jlr.IijLkCCH5.591.LZzZK2VKbdaC9CJC.IOh9cTi8EJmRFwCszjV6qetOLA2Y.z5gA4DERLNME4Gl48taywHu42iKy5X011xy1dA3HYz4hniAlNbg_hHbqK5XDcimrp39oJtqA1IkJ_tswgWf4OYryyrWhixm1rwTUUV4WPkCc2FUwE.XbTCRU8rSnUjtbqO2jnQWuGKRuKdJQh7K2gjJMpZwRHycTZwELuWSKb4HS6xLEc_gaAa08cy04k4ib5v2RP3P2cZ0EexEG1jwm3PGBRmj_3jmju73rNS3nMZqKpgvo7kE3Kf2KmM3OQSFgoWm5S9N53dlWr.6LBySwh9vGuN0ugYDjPNanR.aQdel3wuq0hafdjMcBQehVJbvfZttjfrqRyJVNylQdQsMsLVB0laupFTTWYFYTt2qZdMXfAZQspQtXQ3H8z2R5q4Ws5WBN2DlH5KctJAD8kDPfFEe8JTJAyUeF_AYe5IYd3WqtrSokMDbRFCcoWi6u2bYnKyiYohjWWr0X2v9VphP82x51vTGaIYVtIEpCSSyPtKf.dhvv83qopQqw1UgqtuPPFWzs-
21, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Tue, 15 Dec 2009 05:57:20 PST
21, 24 -- X-Mailer: YahooMailRC/240.3 YahooMailWebService/0.8.100.260964
21, 24 -- References: {200912141657.nBEGvQLY032612-at-ns.microscopy.com}
21, 24 -- Date: Tue, 15 Dec 2009 05:57:20 -0800 (PST)
21, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
21, 24 -- Subject: Re: [Microscopy] SEM bacterial sample prep
21, 24 -- To: microscopy-at-microscopy.com
21, 24 -- In-Reply-To: {200912141657.nBEGvQLY032612-at-ns.microscopy.com}
21, 24 -- MIME-Version: 1.0
21, 24 -- Content-Type: text/plain; charset=iso-8859-1
21, 24 -- Content-Transfer-Encoding: 8bit
21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFDvKna006546
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 15 Dec 2009 08:13:59 -0600
Subject: [Microscopy] viaWWW: Pelleting Cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

I usually centrifuge eucaryotics cells for 3 min at 1500 RPM, but be aware that the centrifugal force depends on the length of the axis (the diameter of the rotor, or more precisely the distance between the central axis and the position of the sample). To disloge the pellet I made myself a simple and efficient tool: I took a fine spatula and I bent it to give a similar curvature as the one of the tube. Then I go along the wall of the tube with the spatula and disloge the pellet by spinning the tube in one hand while using the spatula with the other hand.

Hope it helps,

Stephane



----- Original Message ----
X-from: "l-reyjr-at-northwestern.edu" {l-reyjr-at-northwestern.edu}
To: nizets2-at-yahoo.com
Sent: Tue, December 15, 2009 4:20:09 AM

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at  http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both l-reyjr-at-northwestern.edu as well as  the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: l-reyjr-at-northwestern.edu
Name: Lennell Reynolds

Organization: Northwestern University

Title-Subject: [Filtered] Pelleting Cells

Question: I need to pellet some neutrophils. What is the recommended
rpm and time? How should I dislodge pellet from the side of the
ependorff tube?

Many Thanks
Lennell

  Login Host: 165.124.242.201
---------------------------------------------------------------------------

==============================Original Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Mon Dec 14 21:15:43 2009
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBF3FgwU011268
7, 11 --     for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 21:15:42 -0600
7, 11 -- Mime-Version: 1.0
7, 11 -- Message-Id: {p06240802c74cafc47e00-at-[206.69.208.22]}
7, 11 -- Date: Mon, 14 Dec 2009 21:15:41 -0600
7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: l-reyjr-at-northwestern.edu (by way of MicroscopyListserver)
7, 11 -- Subject: viaWWW: Pelleting Cells
7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
23, 25 -- From nizets2-at-yahoo.com Tue Dec 15 08:13:59 2009
23, 25 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBFEDwoa021451
23, 25 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 08:13:58 -0600
23, 25 -- Received: (qmail 52823 invoked by uid 60001); 15 Dec 2009 14:13:58 -0000
23, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1260886438; bh=lvIDMHYD21YlaA2dAy1b7JnIERnSDQvlX/bX/oGyDOU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=c1u2huW3Ns77HXYuZBzf8e+315f/I8A2sN0L3xPOIlpEf0j0hcGqS6Te8+9/SN2WpNUntkkQa9hkh+fjRUF5+6AJ2EpadvBI8OFFJ2AERsnoFPj1LypDLUVOJbBP7wHWQDvcndP1EuEzvSPdrjBAQdTAbIpvS1J5a5B7yHEXuAo=
23, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
23, 25 -- s=s1024; d=yahoo.com;
23, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
23, 25 -- b=LazcMut5FHODLzbD3pZTL/E2ZVvXXmeS2gC3ZDKOsY/XxolmWQIj84OK4MgB3NE56qTKB05fPGTzFB19Z6aZ1qc6VrAtu8YQiJmt3zbEkO9ipmeB239KGsIlfmN+WsGeegDTYcuQu24Jun5MOhx6rSXW+1UEI0dLiG54qc6SirQ=;
23, 25 -- Message-ID: {443479.50874.qm-at-web110801.mail.gq1.yahoo.com}
23, 25 -- X-YMail-OSG: .B7i3nQVM1lS9rLPhyXq7zRP2I7GYp162BtHbjcejTCQPySv1TtXNGk8nLIyVN1Ebi.oRQYPEFvs2QLpVTIClyw4gM.EBYpdJOPh_GLLsB4CdhHHJkUXOoeZ8pe0hbsdSG09x8PL7o6YZfY1hLPLzYrFi8CfC.XVPZRfrlUSZyabsA1txOP.0eZfSGkWRVP.1yF15Vz2dw7ZX4kMU3tYPetL_BGwSjXCiWkxQhYvCUsTo_UplQy53PL2GS6n6l6xaGXwR9QhpNfGASG31MUWOJuaQbCNSfzjFbED2KF.DwAsiO3a7cWFZ6Mnh.rzqR3KIkZEjgyBY79Oxz2eK9LgRq8DRQnxt_orfEsCa6AXeAzGsIW4TBAi2MqbbOLrUmcmPsLte_HBW5MYaHHtdR6YrSrHdHlE91lEeptFInexbXQZ9joEWgIVJFb.TtF02BKDwo1Wr9JMUTRYjeGy59qL_u_e07GQIeG.7zXAl68y88gdTRdONmYN3zYyulTYbHZKYi3nWSSiAV_.Y_VedqUuRJ7EQA--
23, 25 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Tue, 15 Dec 2009 06:13:58 PST
23, 25 -- X-Mailer: YahooMailRC/240.3 YahooMailWebService/0.8.100.260964
23, 25 -- References: {200912150320.nBF3K9U1027856-at-ns.microscopy.com}
23, 25 -- Date: Tue, 15 Dec 2009 06:13:58 -0800 (PST)
23, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com}
23, 25 -- Subject: Re: [Microscopy] viaWWW: Pelleting Cells
23, 25 -- To: l-reyjr-at-northwestern.edu
23, 25 -- Cc: microscopy-at-microscopy.com
23, 25 -- In-Reply-To: {200912150320.nBF3K9U1027856-at-ns.microscopy.com}
23, 25 -- MIME-Version: 1.0
23, 25 -- Content-Type: text/plain; charset=iso-8859-1
23, 25 -- Content-Transfer-Encoding: 8bit
23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFEDwoa021451
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Tue, 15 Dec 2009 10:11:20 -0600
Subject: [Microscopy] SEM bacterial sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would add one thing to Stephane's setup here. Use the filter holders, and suck down some cells with slight vacuum. The holders come with spacers so if you work quickly enough, you can open up the container and make a filter, cell, spacer, filter sandwich. All cells will be trapped in the center and if solution always moves in the same direction will dry on the effluent filter side/ minimal loss. As most of these filters are stainless Osmium is out


Scott Whittaker
Head NMNH Imaging
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012   MRC104
Washington DC 20013-7012
202-633-0891

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, December 15, 2009 8:59 AM
To: Whittaker, Scott

Danny, I would also go the Phil's way, but in all cases it will be hard to interpret the data since you cannot exclude that post-filtration treatment (fixation, dehydration...) will wash out some cells. To avoid the possibility to wash out the cells during the treatments, I would suggest that you filter all solutions in the direction you used to filter the bacteria. You can use a filter holder (I don't remember who sell it, I think it is SPI but I may be wrong). These holders adapt to syringes. The method works really well, although the rod-shaped bacteria tend to present some artifacts while the coccus are perfectly round (sorry I am not familiar with the english terms, these are the round one). Alternatively, you can cut out a round opening in the cap of an eppendorf tube (1,5 ml), pinch a membrane between tube and cap and incubate the solutions onto the membrane. Then you can simply centrifuge to get rid of the solution, with a fine control of
the centrifuge speed (to avoid complete drying out).
These methods present the advantage of involving no manipulation of the membrane directly and keeping all the bacteria on the filter at all stages.
The last step consists of drying the membrane under the hood (HMDS), then glueing and coating.

Alternatively, you may keep the bacteria wet and use an environmental SEM (just fix them to avoid security problems with pathogens).

Best regards and happy SEMing

Stephane


----- Original Message ----
X-from: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu}
To: nizets2-at-yahoo.com
Sent: Mon, December 14, 2009 5:57:26 PM

Danny,

Ah, I understand. Still, you can't mount the membranes to stubs
before they are dry.

So, for this situation, put the membranes in small petri dishes or a
multi-well plate big enough for the membrane to lie flat, then flood
with glut. Fix, then remove and flood with EtOH. Do this for your
dehydration series. After the final 100% EtOH, do a 1:1 EtOH:HMDS
then 3 X 100% HMDS and air dry. The HMDS **MUST** be used in a fume
hood. Mount the dried membranes.
Note: I routinely run unsupported membranes through CPDs without loss
or problems, but your particular situation may indeed require more
careful handling, therefore HMDS drying.

Just be careful adding and removing the solutions -- I'd do that with
2 pipettes, one to withdraw while adding new solution with the other,
both of them off to the side so they're not squirting/sucking
directly over the membrane.

Phil

} Hi Phil,
}
} I understand about drying being after fixation and dehydration.
} Drying was probaly the wrong word to use because once the bacteria
} have been filtered onto the membranes they are transfered to agar
} slabs which are moist and keep the sample fairly wet so the hour on
} the agar slabs is mainly for the bacteria to settle and attach
} before I treat them so they don't just blow off in the gas flow.
}
} I don't want to count the bacteria with the SEM I just want to
} visually see how they are being deposited e.g stacking or a
} monolayer with minimal amounts of stacking, this is due to the
} treatment I'm using shows potential protection when the bacteria are
} more stacked.
}
} The reason why I mounted the membrane on the stub was to support the
} membrane during the fixing and dryig to minimise damage by
} constantly touching it with tweezers when grading through the
} alcohol. I also don't want the membrane flipping over so I lose
} which side I deposited them on and damaging the bacteria during the
} CPD.
}
} The CPD I have is a E3100 Quroum technologies which is a horizontal
} one and comes with boats to hold the samples but my membranes are
} too big to fit the designed boats so I need some way of securing the
} membranes minimising any risk of damage.
}
} Thanks
}
} Danny
}
}
} On Mon, 14 Dec 2009 10:00:39 -0600
}   oshel1pe-at-cmich.edu wrote:
} }
} }   ----------------------------------------------------------------------------
} }   The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} }   To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} }   On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }   ----------------------------------------------------------------------------
} }   Danny,
} }   If you're drying the bacteria on the membranes, why bother to CPD
} } them? They'll already have lots of drying artifacts. Which may not
} } be relevant, if what you're after is bacterial counts, and you can
} } reliably distinguish air-dried bacteria from schmutz.
} }   Just dry, mount, sputter-coat and examine.
} }   And: fixation is something done before drying, not after. Filter,
} } fix, dehydrate, then dry from 100% EtOH or from HMDS
} } (hexamehtyldisilizane, check the list archives, lots of posts about
} } that), or EtOH to CPD. Amyl acetate isn't needed. Bacteria on
} } fliters generally are good with 3 5-minute soaks in the CPD, after
} } the first flush to get rid of the bulk EtOH.
} }   But mounting the filter membrane onto a stub before doing anything
} } else is putting the cart before the horse while it's still in the
} } stable. Putting the membrane on the stub is done just before
} } sputter-coating.
} }   Phil
} }
} } } Hi all,
} } }
} } } Need some advice on the best method to go with for the preparation
} } } of my biological samples for SEM.
} } }
} } } My samples are prepared by filtrating my bacterial suspension onto
} } } nucleopore track etched membrane filters. After the bacteria have
} } } been filtered the membranes are left on agar slabs to dry for 1
} } } hour so the bacteria can be well attached.
} } }
} } } The bacteria on the membrane will be treated with cold atmospheric
} } } plasma to inactivate the bacteria. I need to use SEM to see how
} } } the bacteria are depositing on the membrane filters at my desired
} } } bacterial concentration.
} } }
} } } The Current preparation protocol procedure I have been using is as follows:
} } }
} } } 1) Filter bacterial suspension on to membrane and allow to
} } } dry/attach for 1 hour.
} } }
} } } 2) Stick the membrane filtered bacteria onto a SEM stub with a
} } } Carbon sticky pad.
} } }
} } } 3) Place the membrane in a 1% gluteraldehyde for 1 hour then 3% overnight.
} } }
} } } 4) Wash in distilled water then grade up to absolute ethanol 20% - 100%
} } }
} } } 5) Grade fully dehydrated sample in amyl acetate up to 100%.
} } }
} } } 6) CPD the samples using liquid CO2 passing it through its critical point.
} } }
} } } The problems I'm having with my method is the best way to secure
} } } the membrane down for the critical point drying because the carbon
} } } sticky pad attachment to the stub works fine until I grade the
} } } sample in Amyl acetate then the sticky pad starts to detach from
} } } the membrane.
} } }
} } } Is it ok to just use ethanol even though it doesn't mix as well
} } } with liquid CO2 and just run a longer time with more flushes in
} } } the CPD?
} } }
} } } Does anyone have any easier suggestions for securing the membrane
} } } down so the membrane doesn't get damaged or effected during the
} } } dehydration and CPD stages?
} } }
} } } Thanks
} } }
} } } Danny
} }   -- Philip Oshel
} }   Microscopy Facility Supervisor
} }   Biology Department
} }   024C Brooks Hall
} }   Central Michigan University
} }   Mt. Pleasant, MI 48859
} }   (989) 774-3576

==============================Original Headers==============================
6, 27 -- From oshel1pe-at-cmich.edu Mon Dec 14 10:51:51 2009
6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
6, 27 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBEGpo6Q025632
6, 27 --     for {Microscopy-at-microscopy.com} ; Mon, 14 Dec 2009 10:51:51 -0600
6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74])
6, 27 --     by ob4.cmich.edu (8.14.3/8.14.3/Debian-5) with ESMTP id nBEGoXmR031860;
6, 27 --     Mon, 14 Dec 2009 11:51:23 -0500
6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959);
6, 27 --     Mon, 14 Dec 2009 11:51:21 -0500
6, 27 -- Mime-Version: 1.0
6, 27 -- Message-Id: {f06240803c74c1bacc9d2-at-[141.209.160.249]}
6, 27 -- In-Reply-To: {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- References: {200912141600.nBEG0dZw010972-at-ns.microscopy.com}
6, 27 --  {web-8023751-at-frim.lboro.ac.uk}
6, 27 -- Date: Mon, 14 Dec 2009 11:51:18 -0500
6, 27 -- To: D.L.Bayliss-at-lboro.ac.uk
6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
6, 27 -- Subject: Re: [Microscopy] Re: SEM bacterial sample prep
6, 27 -- Cc: Microscopy-at-microscopy.com
6, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
6, 27 -- X-OriginalArrivalTime: 14 Dec 2009 16:51:21.0943 (UTC) FILETIME=[AA402E70:01CA7CDD]
6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
6, 27 -- X-Spam-Score: -3.40 () [Hold at 6.00] L_EXCH_MF,L_USD,PORN_RP_SUCKING,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5)
6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6
6, 27 -- X-CanItPRO-Stream: default
6, 27 -- X-Canit-Stats-ID: 24418988 - 049e1da62fd1 - 20091214
6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================






==============================Original Headers==============================
21, 24 -- From nizets2-at-yahoo.com Tue Dec 15 07:57:21 2009
21, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224])
21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBFDvKna006546
21, 24 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 07:57:21 -0600
21, 24 -- Received: (qmail 42297 invoked by uid 60001); 15 Dec 2009 13:57:20 -0000
21, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1260885440; bh=b17FVeZnFaKCECNwLj9VvGqb72rpM9ONPSNmhux9ApM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=n5j7xsaqSvbFZr0zgllDSe2lMrrGrSvKrfUGEBternoUNmt3lw8ZvvxobsosXVcQrf/KWIhAPDQ3USTYlb2T2E6uMy3taTzCzmVQC6FeE/QwpViqt71RN2qlqc8k8wO1KTMNv3vnBQ0cgQou0vRSGgz7hj7s0Nha0g/RNm05/M8=
21, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
21, 24 -- s=s1024; d=yahoo.com;
21, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding;
21, 24 -- b=xmoR3SA35IiuvF7jwB2erksAZ+u3UxBw2E7u+bJok+/QuaC4lcBPraAu0hBg9be7HkTw520kt5W74ogPrAKDfFlA1+ZsziB0UBbWYAG8/A7S5xbuxLXxvKnQJx55dP4fDG2/0g1TC11juzNBQ5pPG44hBQw8z50ByoTvDVkR/wk=;
21, 24 -- Message-ID: {408386.41792.qm-at-web110801.mail.gq1.yahoo.com}
21, 24 -- X-YMail-OSG: i2VzE4QVM1mvJqFDaU617qnOSonhA1Yqa4hlQuFJYNBpR4hFI52FJFcsoxP0OPWRGNYhQaRzrGqUf3FVkTrsvTGb9f27OVhKr3MdEtmK5caQXy1jlr.IijLkCCH5.591.LZzZK2VKbdaC9CJC.IOh9cTi8EJmRFwCszjV6qetOLA2Y.z5gA4DERLNME4Gl48taywHu42iKy5X011xy1dA3HYz4hniAlNbg_hHbqK5XDcimrp39oJtqA1IkJ_tswgWf4OYryyrWhixm1rwTUUV4WPkCc2FUwE.XbTCRU8rSnUjtbqO2jnQWuGKRuKdJQh7K2gjJMpZwRHycTZwELuWSKb4HS6xLEc_gaAa08cy04k4ib5v2RP3P2cZ0EexEG1jwm3PGBRmj_3jmju73rNS3nMZqKpgvo7kE3Kf2KmM3OQSFgoWm5S9N53dlWr.6LBySwh9vGuN0ugYDjPNanR.aQdel3wuq0hafdjMcBQehVJbvfZttjfrqRyJVNylQdQsMsLVB0laupFTTWYFYTt2qZdMXfAZQspQtXQ3H8z2R5q4Ws5WBN2DlH5KctJAD8kDPfFEe8JTJAyUeF_AYe5IYd3WqtrSokMDbRFCcoWi6u2bYnKyiYohjWWr0X2v9VphP82x51vTGaIYVtIEpCSSyPtKf.dhvv83qopQqw1UgqtuPPFWzs-
21, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Tue, 15 Dec 2009 05:57:20 PST
21, 24 -- X-Mailer: YahooMailRC/240.3 YahooMailWebService/0.8.100.260964
21, 24 -- References: {200912141657.nBEGvQLY032612-at-ns.microscopy.com}
21, 24 -- Date: Tue, 15 Dec 2009 05:57:20 -0800 (PST)
21, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com}
21, 24 -- Subject: Re: [Microscopy] SEM bacterial sample prep
21, 24 -- To: microscopy-at-microscopy.com
21, 24 -- In-Reply-To: {200912141657.nBEGvQLY032612-at-ns.microscopy.com}
21, 24 -- MIME-Version: 1.0
21, 24 -- Content-Type: text/plain; charset=iso-8859-1
21, 24 -- Content-Transfer-Encoding: 8bit
21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFDvKna006546
==============================End of - Headers==============================


==============================Original Headers==============================
30, 28 -- From WHITTAKS-at-si.edu Tue Dec 15 10:11:20 2009
30, 28 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178])
30, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFGBJsH008505
30, 28 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 10:11:20 -0600
30, 28 -- Received: from SI-MSEHUB-N02.US.SINET.SI.EDU (si-msehub-n02.us.sinet.si.edu [160.111.49.126])
30, 28 -- by si-mailout04.si.edu (Postfix) with ESMTP id B6BDA7EDD
30, 28 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 11:06:40 -0500 (EST)
30, 28 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::31c0:a8cf:a171:1a3d]) by
30, 28 -- SI-MSEHUB-N02.US.SINET.SI.EDU ([fe80::dfb:a2f1:b0f7:9c85%11]) with mapi; Tue,
30, 28 -- 15 Dec 2009 11:11:19 -0500
30, 28 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
30, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
30, 28 -- Date: Tue, 15 Dec 2009 11:08:32 -0500
30, 28 -- Subject: RE: [Microscopy] Re: SEM bacterial sample prep
30, 28 -- Thread-Topic: [Microscopy] Re: SEM bacterial sample prep
30, 28 -- Thread-Index: Acp9jrlvnFr131yYT9Kyoyfu0nxLpAAEbCqA
30, 28 -- Message-ID: {038C04650A93A14381E944A473695F618E152BAE83-at-SI-MSEV03.US.SINET.SI.EDU}
30, 28 -- References: {200912151358.nBFDwkPh008681-at-ns.microscopy.com}
30, 28 -- In-Reply-To: {200912151358.nBFDwkPh008681-at-ns.microscopy.com}
30, 28 -- Accept-Language: en-US
30, 28 -- Content-Language: en-US
30, 28 -- X-MS-Has-Attach:
30, 28 -- X-MS-TNEF-Correlator:
30, 28 -- acceptlanguage: en-US
30, 28 -- Content-Type: text/plain; charset="iso-8859-1"
30, 28 -- MIME-Version: 1.0
30, 28 -- Content-Transfer-Encoding: 8bit
30, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFGBJsH008505
==============================End of - Headers==============================




From: kjmorris-at-well.ox.ac.uk
Date: Tue, 15 Dec 2009 10:14:48 -0600
Subject: [Microscopy] viaWWW: Printing digital EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have written a short article for the Royal Microscopy Society's InFocus
magazine about scanning TEM negatives/colour slide film 'on a budget' - you
can download it from here if you wish:
http://www.well.ox.ac.uk/cytogenetics/downloads/InfocusScanningFilmKJM
It's not really out of date as scanning film now a niche activity with the
rise of digital cameras and not much has changed - although it just mentions
the release of the superior Epson V700/V750. So yes the V700 or preferably
V750 Pro scanners are still probably the best choice at present for scanning
large format film cost effectively. See more details at:
http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm
And keep the TEM negatives, they are good for 500 years storage thanks to
that polyester substrate, unlike the scanned digital file.


Regarding printing photos:

I have to say that for 'on a budget', a decent prosumer photographic printer
[under £500] from HP seems more than adequate [as most, if not all, users
won't print out much anyway once they have the scanned the TEM negative onto
the PC. That said, and for the above reason, we don't even bother to have a
specialist photo-printer here, even though they are relatively cheap to buy
and use [comparable to commercial printing rates] and produce excellent
results [that last 100 years+]. Our users just use the standard HP office
colour laser - it's output's utterly naff compared to a photoprinter image
on proper photopaper, but it's adequate for showing images to colleagues if
there's no PC handy for viewing the original scanned digital image.

Downside of a photoprinter is that people might over-use it and the Vivera
inks are more expensive than vintage champagne [I'd never use anything but
the recommended HP cartridges/Vivera inks]. But if you must print those
6,000 prints a year, it will be expensive with Vivera inks [I suppose it is
only 30 prints a day, and the inks will cost more than the printer, so
wearing out a printer every few years won't be a problem, and the heads are
replaceable - but read the HP customer reviews, they may put you off
regarding cost and reliability [often just paper loading problems, so load
carefully and update firmware] - however you might want to look for a more
commercial hi-volume printing solution.

HP photo printers:
http://h10010.www1.hp.com/wwpc/uk/en/ho/WF02d/18972-18972-3328063.html
http://www.photo-i.co.uk/Reviews/interactive/HPB8850/page-1.html review of
cheaper model not the B9180
http://www.photo-i.co.uk/Reviews/interactive/HP%20B9180/page-1.htm review
HP's top fineart photograph printer - the better choice
"And when paired with HP Photosmart printers and HP Premium Plus Photo
paper, photos printed with Vivera inks resist fading for over 100 years!"
http://www.hp.com/united-states/consumer/digital_photography/print_better_ph
otos/tips/preventing_photo_fade.html

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy


-----Original Message-----
X-from: cruzetti-at-hotmail.com [mailto:cruzetti-at-hotmail.com]
Sent: 11 December 2009 02:10
To: kjmorris-at-well.ox.ac.uk

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both cruzetti-at-hotmail.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: cruzetti-at-hotmail.com
Name: Jeffrey Crews

Organization: Pathology Associates North Carolina

Title-Subject: [Filtered] Printing digital EM

Question: Hello-

My lab is looking into scanning our 4489 negatives and printing them
as digital prints, rather than continuing to use traditional photo
paper and chemistry.

} From searching the archives it looks like the Epson 700 or 750 are
} good choices for the scanning end, but I am still at a loss for a
} good B&W-only photo printer.

We'd need to produce prints that are as permanent and high-res as
traditional prints, if that's possible. We're making 6000+ prints a
year.

Any insights or brand names, etc would be highly appreciated. We're
not pulling the trigger on this yet, but I'd definitely like to start
pulling together some info. Many thanks.

JC

Login Host: 207.247.42.130
---------------------------------------------------------------------------

==============================Original Headers==============================
11, 11 -- From zaluzec-at-microscopy.com Thu Dec 10 19:58:14 2009
11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
nBB1wD0Z016228
11, 11 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2009 19:58:14
-0600
11, 11 -- Mime-Version: 1.0
11, 11 -- Message-Id: {p06240801c747579eceef-at-[206.69.208.22]}
11, 11 -- Date: Thu, 10 Dec 2009 19:58:12 -0600
11, 11 -- To: microscopy-at-microscopy.com
11, 11 -- From: cruzetti-at-hotmail.com (by way of MicroscopyListserver)
11, 11 -- Subject: viaWWW: Printing digital EM
11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
29, 23 -- From kjmorris-at-well.ox.ac.uk Tue Dec 15 10:14:48 2009
29, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2])
29, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFGEldO012570
29, 23 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 10:14:48 -0600
29, 23 -- Received: from dhcp285.well.ox.ac.uk ([129.67.45.128] helo=CytoWhizz)
29, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52)
29, 23 -- id 1NKa2w-0004a9-Qa; Tue, 15 Dec 2009 16:14:46 +0000
29, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk}
29, 23 -- To: {cruzetti-at-hotmail.com}
29, 23 -- Cc: {microscopy-at-microscopy.com}
29, 23 -- References: {200912110209.nBB29X7e003502-at-ns.microscopy.com}
29, 23 -- In-Reply-To: {200912110209.nBB29X7e003502-at-ns.microscopy.com}
29, 23 -- Subject: RE: [Microscopy] viaWWW: Printing digital EM
29, 23 -- Date: Tue, 15 Dec 2009 16:14:46 -0000
29, 23 -- Message-ID: {002901ca7da1$b8346a90$289d3fb0$-at-ox.ac.uk}
29, 23 -- MIME-Version: 1.0
29, 23 -- Content-Type: text/plain;
29, 23 -- charset="iso-8859-1"
29, 23 -- X-Mailer: Microsoft Office Outlook 12.0
29, 23 -- Thread-Index: Acp6BvtrlUhZAWE4RsuZiAq6y1ffVgDjZdAg
29, 23 -- Content-Language: en-gb
29, 23 -- Content-Transfer-Encoding: 8bit
29, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFGEldO012570
==============================End of - Headers==============================




From: John.Mardinly-at-wdc.com
Date: Tue, 15 Dec 2009 10:59:20 -0600
Subject: [Microscopy] Image J

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone used Image J for grain size analysis? Our department has a need
for automated grain size measurement, but no budget for professional
software or systems. The Image Processing Handbook by John Russ has some
strategic guidelines, but no actual recipe. There are a number of macros in
Image J, but I have not been able to put together a sequence that works for
detecting grain boundaries reliably. Thanks.

John Mardinly
Western Digital


==============================Original Headers==============================
3, 23 -- From John.Mardinly-at-wdc.com Tue Dec 15 10:59:20 2009
3, 23 -- Received: from wdscexfe02.sc.wdc.com (wdscexfe02.sc.wdc.com [129.253.170.52])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFGxKFH006425
3, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 10:59:20 -0600
3, 23 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
3, 23 -- Tue, 15 Dec 2009 08:59:19 -0800
3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
3, 23 -- Content-class: urn:content-classes:message
3, 23 -- MIME-Version: 1.0
3, 23 -- Content-Type: text/plain;
3, 23 -- charset="us-ascii"
3, 23 -- Subject: Image J
3, 23 -- Date: Tue, 15 Dec 2009 08:59:19 -0800
3, 23 -- Message-ID: {34061960C62E274C8AF1DCAA6565555805E1194E-at-wdksjexbe01.msj.wdc.com}
3, 23 -- X-MS-Has-Attach:
3, 23 -- X-MS-TNEF-Correlator:
3, 23 -- Thread-Topic: Image J
3, 23 -- Thread-Index: Acp9p/D1gHVcgOysQcqdyfBALUSDZA==
3, 23 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
3, 23 -- To: {Microscopy-at-microscopy.com}
3, 23 -- X-OriginalArrivalTime: 15 Dec 2009 16:59:19.0228 (UTC) FILETIME=[F125D7C0:01CA7DA7]
3, 23 -- Content-Transfer-Encoding: 8bit
3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBFGxKFH006425
==============================End of - Headers==============================




From: Noel.Goldsmith-at-dsto.defence.gov.au
Date: Tue, 15 Dec 2009 16:44:16 -0600
Subject: [Microscopy] Re: Image J [SEC=UNCLASSIFIED]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,
Hi,
In brief, not yet. I am working on porting my old NIH Image Macros, but also
have to do my normal job.
There is an excellent book by John Russ (among other excellent books),
Plenum published the textbook Computer Assisted Microscopy (1990, ISBN
number 0-306-43410-5), which contains the algorithms below (or that was my
intention! Maybe you should check).
I also wish to acknowledge the help and advice given by contributors to the
NIH Image list and the ImageJ list.

The algorithms can be found buried in some NIH image macros which I am
pasting in.
These contains 3 methods.
The linear intercept, the blob detection, and the triple point count.
Linear intercept is a method to count the intercepts between a circle and
the grain boundaries.
Blob detection uses the body of the grains as blobs, uses watershed to
separate them and counts and measures each grain as an object.
The triple point count relies on detecting and counting the triple points
left after skeletonising the grain boundaries, and performing binary
processing so the triple points are left. And then counts them and gives a
grain size.
A manual count is pretty quick using a circle on an image. (several times).
The images need to be scaled.

These will need to be ported into ImageJ as macros in the first instance, to
see how it all goes and then to be converted to Plugins.

In my experience the preparation of a good specimen, polishing and etching,
followed by the taking of a good image is the most difficult part.
And if you have a material such as mild steel, some parts of boundaries can
be almost invisible. Techniques such as dark field, oblique illumination and
interference contrast may help. Or whatever you do with your instruments,
which I see might be a TEM or an SEM. Any method which can show the body of
the grain as distinctly different from the boundary may be used. And of
course, with digital images, it is possible to combine images produced in
different ways.
I would recommend that a "Flat field" image be taken first which can be used
in ImageJ to flatten the actual image.
Some cameras have this function as part of their software.
Of course with metallography, obtaining a perfect mirror to use for a flat
field object, is a challenge. I have found that a piece of glass can be
good. And a coated piece is also good.
Maybe you could use a bit of a hard disc platter or even a blank CD. It is
essential that the flat field is taken using the same lenses, focus and
light settings.
There has been a bit of discussion regarding thresholding on the imagej
list, which you could dig into.
Also the extra functionality of the watershed, which allows some parameters
to be varied, should mean that watershed works better. But will need more
driving. In fact, I now think that the watershed may do the trick.
But the triple point method is likely to be robust, as the triple points are
often the most obvious part of the image.
Hope this helps
Noel

Noel Goldsmith
Air Vehicles Division
DSTO
506 Lorimer Street
Port Melbourne
3207 Victoria
AUSTRALIA
613 96267527
0428364003
noel.goldsmith-at-dsto.defence.gov.au
--




macro 'CountNeighbours';

var
i:integer;
begin
scaleconvolutions(true);
newtextwindow('kernel',100,100);
writeln('1 1 1');
writeln('1 43 1');
writeln('1 1 1');
Convolve('');
SelectWindow('Kernel');
Dispose;
for i:=0 to 8 do begin
changevalues(5*i,5*i,0);
ChangeValues(215+5*i,215+5*i,i);
end;
end;

macro 'ColourNeighbours';
var i:integer;
begin
RedLut[1]:=255;BlueLut[1]:=0; GreenLut[1]:=0;
RedLut[2]:=0;BlueLut[2]:=0; GreenLut[1]:=0;
for i := 3 to 8 do begin
RedLut[i]:=0;BlueLut[i]:=0; GreenLut[i]:=255;
end;
RedLut[3]:=0; BlueLut[3]:=127; greenLut[3]:=0;
RedLut[4]:=0; BlueLut[4]:=255; greenLut[4]:=0;
Updatelut;
end;

Macro 'Count Nodes';
var NumNodes:integer;
begin
resetcounters;
setoptions('area,x-y center');
setdensityslice(3,4);
makeBinary;
analyzeParticles;
NumNodes:=rCount;
PutMessage('There are ',NumNodes,' Intersections');
end;

Macro 'Compute Grainsize by Triple Points';
{Uses number of triple points in the area in square mm}
{and the formula {GrainSize:=ln(((NodeCount/2)-1)/PicArea)/(ln(2))-2.95}
var GrainSize:real;
i:integer;
myscale,myAspect:real;
myunit:string;
PicArea:real;
NodeCount:integer;
begin
Open('');
resetcounters;
GetScale(myscale,myunit,myAspect);
if (myunit {} 'mm') then begin
PutMessage('The image must be scaled in mm');
Exit('Calibrate Image by Using Set Scale');
end;
scaleconvolutions(true);
newtextwindow('kernel',100,100);
writeln('1 1 1');
writeln('1 43 1');
writeln('1 1 1');
Convolve('');
SelectWindow('Kernel');
Dispose;
for i:=0 to 8 do begin
changevalues(5*i,5*i,0);
ChangeValues(215+5*i,215+5*i,i);
end;
RedLut[1]:=255;BlueLut[1]:=0; GreenLut[1]:=0;
RedLut[2]:=0;BlueLut[2]:=0; GreenLut[1]:=0;
for i := 3 to 8 do begin
RedLut[i]:=0;BlueLut[i]:=0; GreenLut[i]:=255;
end;
RedLut[3]:=0; BlueLut[3]:=127; greenLut[3]:=0;
RedLut[4]:=0; BlueLut[4]:=255; greenLut[4]:=0;
Updatelut;

SelectAll;
Measure;
PicArea:=rArea[1];
ResetCounter;
setoptions('area,x-y center, headings');
setDensityslice(3,4);
MakeBinary;
AnalyzeParticles('');
NodeCount:=rCount;
GrainSize:=ln(((NodeCount/2)-1)/PicArea)/(ln(2))-2.95;
RevertToSaved;
close;
NewTextWindow('Grain Size Results (Triple Pts)',400,200);
SetFontSize(18);
Moveto(30,20); Writeln('ASTM Grain Size');
Writeln;
Writeln('ASTM Grain Size:= ',GrainSize);
Writeln('Number of Triple Points :'Nodecount);
Writeln('Image Area :',PicArea,' square mm');
SelectWindow('Grain Size Results');

end;


Macro 'Grain Size by Intercept';
{Measures grain size by counting intersections of}
{Circular lines with boundaries}
{Std method is put a circle down on image and }
{count intersections until you get at least 35}
{then compute G=-10-6.64logL3}
{L3=Lt/(P*M)}
{Where G = Grain size, }
{Lt is total length of test line in cm}
{P is number if intersections}
{M is magnification of image}
{This implies the cms are measured at }
{the scale of the image, for images in }
{which we know the true value of a cm then }
{the mag is 1}
var GrainSize:real;
i:integer;
Interceptcount:integer;
myscale,myAspect,Mag:real;
myunit:string;
myimage:string;
mypicid1,mypicid2,mypicid3:integer;
mypicid4,mypicid5,mypicid6:integer;

PicArea,NumPerArea,Circlelength:real;
myunit:string;
PicArea,Lthree:real;
NodeCount:integer;
top,left,width,height,pwidth,pheight:integer;
begin
Open('');
mypicid1:=pidnumber;
getPicSize(pwidth,pheight);
PicArea:=pwidth*pheight;
setnewsize(pwidth,pheight);
width:=round(pwidth/2);height:=width;
left:=20; Top:=20;
resetcounters;
GetScale(myscale,myunit,myAspect);
PicArea:=pwidth/myscale*pheight/myscale;
if (myunit {} 'mm') then begin
PutMessage('The image must be scaled in mm');
Exit('Calibrate Image by Using Set Scale');
end;
{put circles onto grain images}
setoptions('area,x-y center,length, headings');
MakeNewWindow('Mask');
mypicid2:=pidnumber;
setforegroundcolor(255);
makeovalroi(left,top,width,height);
DrawBoundary;
{measure length}
Selectall;
setThreshold(255);
Makebinary;
AutoOutline(10,30);Measure;
Circlelength:=rLength[1]; {This will be mmm }
CircleLength:=CircleLength/10; {This will be CM}
Selectall;
Copy;
{NOW PASTE WITH AND ONTO IMAGE OF GRAINS}
SELECTPIC(mypicid1);
Setoption; paste;doand;
ResetCounter;

setoptions('area,x-y center, headings');
setThreshold(255);
MakeBinary;
AnalyzeParticles('');
Interceptcount:=rCount;
Lthree:=CircleLength/InterceptCount;
Lthree:=ln(lthree)/ln(10);
GrainSize:=-10.0-6.64*Lthree;
RevertToSaved;
SelectPic(mypicid2);
Selectall;Clear;
makeovalroi(left+width-30,top,width,height);
DrawBoundary; Selectall;Copy;
SELECTPIC(mypicid1);
Setoption; paste;doand;
setThreshold(255);
MakeBinary;
AnalyzeParticles('');
Interceptcount:=rCount;
Lthree:=CircleLength*2/InterceptCount;
Lthree:=ln(lthree)/ln(10);
GrainSize:=-10.0-6.64*Lthree;
RevertToSaved;
{close;}
NewTextWindow('Grain Size Results (intercept)',400,200);
SetFontSize(18);
Moveto(30,20); Writeln('ASTM Grain Size');
Writeln;
Writeln('ASTM Grain Size:= ',GrainSize);
Writeln('Number of Intercepts :'Interceptcount);
Writeln('Image Area :',PicArea:2:4,' square mm');
Writeln('Circle length :',CircleLength,' mm');
SelectWindow('Grain Size Results');

end;


Macro 'Grain Size by Areas';
{Uses areas of blobs in sq mm and gives number}
{per square mm}
{Formula is G=(3.22log(Na.M^2))-2.95}
{Note that this
magnification will be 1 for scaled images}
{assuming 72 dots per inch as standard}
{2.834 dots or pixels per mm is 1 time}
{So the mag is the Number of pixels per mm/2.83464}var GrainSize:real;
i:integer;
Interceptcount:integer;
myscale,myAspect,Mag:real;
myunit:string;
myimage:string;
mypicid1,mypicid2,mypicid3:integer;
mypicid4,mypicid5,mypicid6:integer;
NumGrains:integer;
PicArea,NumPerArea,Circlelength:real;
myunit:string;
PicArea,Lthree:real;
NodeCount:integer;
top,left,width,height,pwidth,pheight:integer;
begin
Open('');
mypicid1:=pidnumber;
getPicSize(pwidth,pheight);
setnewsize(pwidth,pheight);
width:=round(pwidth/2);height:=round(pheight/2);
left:=20; Top:=20;
resetcounters;
GetScale(myscale,myunit,myAspect);
if (myunit {} 'mm') then begin
PutMessage('The image must be scaled in mm');
Exit('Calibrate Image by Using Set Scale');
end;
if (myunit {} 'mm') then begin
PutMessage('The image must be scaled in mm');
Exit('Calibrate Image by Using Set Scale');
end;
Mag:=myscale/2.83464;
selectall;
Measure;
PicArea:=rArea[1];
resetcounters;
Invert;
Duplicate('InvertCopy');
Mypicid2:=pidnumber;
SelectPic(Mypicid1);
Binary('Watershed');

Analyzeparticles('');
NumGrains:=rcount;
NumPerArea:=NumGrains/PicArea;
GrainSize:=(3.22*ln(NumPerArea)/ln(10))-2.95;
NewTextWindow('Grain Size Results (area) ',400,200);
Writeln('Grain Size results');
Writeln;
Writeln('Grain Size : ',GrainSize);
Writeln('Number of Grains: ',NumGrains);
Writeln('Area of Section: ',PicArea,' Square mm');
Writeln('Grains Per Unit Area: ',NumPerArea);
Writeln('Magnification: ',Mag);
Writeln('Scale factor: ',myscale);
Writeln('Unit : ',myunit);
end;



On 16/12/09 4:06 AM, "John.Mardinly-at-wdc.com" {John.Mardinly-at-wdc.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Has anyone used Image J for grain size analysis? Our department has a need
} for automated grain size measurement, but no budget for professional
} software or systems. The Image Processing Handbook by John Russ has some
} strategic guidelines, but no actual recipe. There are a number of macros in
} Image J, but I have not been able to put together a sequence that works for
} detecting grain boundaries reliably. Thanks.
}
} John Mardinly
} Western Digital
}
}
} ==============================Original Headers==============================
} 3, 23 -- From John.Mardinly-at-wdc.com Tue Dec 15 10:59:20 2009
} 3, 23 -- Received: from wdscexfe02.sc.wdc.com (wdscexfe02.sc.wdc.com
} [129.253.170.52])
} 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} nBFGxKFH006425
} 3, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 10:59:20 -0600
} 3, 23 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by
} wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.3959);
} 3, 23 -- Tue, 15 Dec 2009 08:59:19 -0800
} 3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
} 3, 23 -- Content-class: urn:content-classes:message
} 3, 23 -- MIME-Version: 1.0
} 3, 23 -- Content-Type: text/plain;
} 3, 23 -- charset="us-ascii"
} 3, 23 -- Subject: Image J
} 3, 23 -- Date: Tue, 15 Dec 2009 08:59:19 -0800
} 3, 23 -- Message-ID:
} {34061960C62E274C8AF1DCAA6565555805E1194E-at-wdksjexbe01.msj.wdc.com}
} 3, 23 -- X-MS-Has-Attach:
} 3, 23 -- X-MS-TNEF-Correlator:
} 3, 23 -- Thread-Topic: Image J
} 3, 23 -- Thread-Index: Acp9p/D1gHVcgOysQcqdyfBALUSDZA==
} 3, 23 -- From: "John Mardinly" {John.Mardinly-at-wdc.com}
} 3, 23 -- To: {Microscopy-at-microscopy.com}
} 3, 23 -- X-OriginalArrivalTime: 15 Dec 2009 16:59:19.0228 (UTC)
} FILETIME=[F125D7C0:01CA7DA7]
} 3, 23 -- Content-Transfer-Encoding: 8bit
} 3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id nBFGxKFH006425
} ==============================End of - Headers==============================


IMPORTANT: This email remains the property of the Australian Defence Organisation and is subject to the jurisdiction of section 70 of the CRIMES ACT 1914. If you have received this email in error, you are requested to contact the sender and delete the email.


==============================Original Headers==============================
30, 35 -- From Noel.Goldsmith-at-dsto.defence.gov.au Tue Dec 15 16:44:15 2009
30, 35 -- Received: from digger1.defence.gov.au (digger1.defence.gov.au [203.5.217.4])
30, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBFMiDdu031723
30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 16:44:14 -0600
30, 35 -- Received: from ednmsw520.dsto.defence.gov.au (ednmsw520.dsto.defence.gov.au [131.185.68.60])
30, 35 -- by digger1.defence.gov.au (DSTO/DSTO) with ESMTP id nBFMg7K6027374
30, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 09:12:07 +1030 (CST)
30, 35 -- Received: from fmbex510.dsto.defence.gov.au (fmbex510.dsto.defence.gov.au) by
30, 35 -- ednmsw520.dsto.defence.gov.au (Clearswift SMTPRS 5.3.2) with ESMTP id
30, 35 -- {T92a32ce0cf83b9443cba4-at-ednmsw520.dsto.defence.gov.au} ; Wed, 16 Dec
30, 35 -- 2009 09:14:12 +1030
30, 35 -- Received: from fmbex511.dsto.defence.gov.au ([131.185.134.21]) by
30, 35 -- fmbex510.dsto.defence.gov.au with Microsoft SMTPSVC(6.0.3790.3959);
30, 35 -- Wed, 16 Dec 2009 09:44:12 +1100
30, 35 -- Received: from 131.185.112.211 ([131.185.112.211]) by
30, 35 -- fmbex511.dsto.defence.gov.au ([131.185.134.21]) with Microsoft
30, 35 -- Exchange Server HTTP-DAV ; Tue, 15 Dec 2009 22:44:11 +0000
30, 35 -- User-Agent: Microsoft-Entourage/11.4.0.080122
30, 35 -- Date: Wed, 16 Dec 2009 09:38:05 +1100
30, 35 -- Subject: Re: [Microscopy] Image J [SEC=UNCLASSIFIED]
30, 35 -- From: "Goldsmith, Noel" {Noel.Goldsmith-at-dsto.defence.gov.au}
30, 35 -- To: {John.Mardinly-at-wdc.com} , {Microscopy-at-microscopy.com}
30, 35 -- Message-ID: {C74E5AFD.5A0C%Noel.Goldsmith-at-dsto.defence.gov.au}
30, 35 -- Thread-Topic: [Microscopy] Image J [SEC=UNCLASSIFIED]
30, 35 -- Thread-Index: Acp910RAgwTdTOnKEd6GVAAX8smQog==
30, 35 -- In-Reply-To: {200912151706.nBFH6UmT020572-at-ns.microscopy.com}
30, 35 -- Mime-version: 1.0
30, 35 -- Content-type: text/plain; charset="us-ascii"
30, 35 -- Content-transfer-encoding: 7bit
30, 35 -- X-OriginalArrivalTime: 15 Dec 2009 22:44:12.0277 (UTC)
30, 35 -- FILETIME=[1F2A8A50:01CA7DD8]
30, 35 -- X-TM-AS-Product-Ver: SMEX-8.0.0.1285-6.000.1038-17072.000
30, 35 -- X-TM-AS-Result: No--30.543300-0.000000-31
30, 35 -- X-TM-AS-User-Approved-Sender: Yes
30, 35 -- X-TM-AS-User-Blocked-Sender: No
==============================End of - Headers==============================




From: jwdeng-at-imr.ac.cn
Date: Tue, 15 Dec 2009 22:44:46 -0600
Subject: [Microscopy] viaWWW: Rotational average ring pattern

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jwdeng-at-imr.ac.cn as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jwdeng-at-imr.ac.cn
Name: J.W. Deng

Title-Subject: [Filtered] Rotational average ring pattern

Question: Dear Listers,
Recently, I want to extract the rotational average intensity
distribution from a ring diffraction pattern. I know there is a
DigitalMicrograph script can do rotational average, however it can't
change the integral angle. So I would like to know what software or
source code can be used to perform the rotational average of an
image. Ii is better if I can change the center and angular range of
the ring pattern, because the beam stop is used usually.

Many Thanks
J.W. Deng

Login Host: 210.72.130.175
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Dec 15 22:44:44 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBG4ihie023967
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 22:44:43 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c74e16297f69-at-[206.69.208.22]}
6, 11 -- Date: Tue, 15 Dec 2009 22:44:41 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: jwdeng-at-imr.ac.cn (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Rotational average ring pattern
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: pal.baggethun-at-elkem.no
Date: Wed, 16 Dec 2009 08:51:54 -0600
Subject: [Microscopy] viaWWW: Re: Rotational average ring pattern

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pal.baggethun-at-elkem.no as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pal.baggethun-at-elkem.no
Name: P. Baggethun

Organization: Elkem Solar Research

Title-Subject: [Filtered] Re: Rotational average ring pattern

Question: You can consider the public domain software ImageJ
http://rsbweb.nih.gov/ij/

with the following plugins:

Radial Profile Plot
http://rsbweb.nih.gov/ij/plugins/radial-profile.html
"This plugin produces a profile plot of normalized integrated
intensities around concentric circles as a function of distance from
a point in the image. The point is automatically defined as the
center of the rectangle that bounds the current ROI"

Radial Profile Extended
http://rsbweb.nih.gov/ij/plugins/radial-profile-ext.html
"The plugin lets you choose the starting angle and integration angle
over which the integration on the defined Roi is done. The
integration will be done over an area defined by : starting angle +/-
integration angle. The size and the position of the Roi can be
defined and modified by either using the plugin menu or shortkeys on
keyboard. Additionnally, the integration calculation can be done over
a whole stack."


Login Host: 212.4.33.171
---------------------------------------------------------------------------

==============================Original Headers==============================
10, 11 -- From zaluzec-at-microscopy.com Wed Dec 16 08:51:53 2009
10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGEprLG007054
10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 08:51:53 -0600
10, 11 -- Mime-Version: 1.0
10, 11 -- Message-Id: {p06240800c74ea469d70f-at-[206.69.208.22]}
10, 11 -- Date: Wed, 16 Dec 2009 08:51:52 -0600
10, 11 -- To: microscopy-at-microscopy.com
10, 11 -- From: pal.baggethun-at-elkem.no (by way of MicroscopyListserver)
10, 11 -- Subject: viaWWW: Re: Rotational average ring pattern
10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: FMonson-at-wcupa.edu
Date: Wed, 16 Dec 2009 09:58:47 -0600
Subject: [Microscopy] Re: biological TEM vs. materials TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For me, there are no clear answers anymore to your question - not to argue any points made thus far by others. I will use, by virtue of familiarity, the TEM's of which I have most knowledge.

For the record, I have spent the past 7 years with a FEI Quanta 400 Environmental SEM with Oxford INCA EDS with maximum automation and a FEI Tecnai G2 12T (120 kV Twin) with resolution at 0.2nm at installation. Both of these instruments have tungsten emitters. My prior history with TEM's is limited to an Hitachi HS-7 and a JEOL 100C - both of these between 1974 and 1986. Thus, my real expertise, such as it is, derives from the last 7 years of my life with FEI instruments. You may, quite justifiably, take me with a large grain of salt.

What is clear to me at this point in time is that two criteria are pertinent in selection of a TEM (or any other instrument): 1) the use for which the microscope was designed; and 2) the use to which it is to be put. In contrast, while certain specification alterations can narrow the full capacity of a TEM design, my recent experience has taught this biologist that there is no danger in chasing the lowest possible resolution and the lowest gun and column pressures with the funding available. Even a current 200 kV instrument can apparently be coaxed to perform beautifully at 60-80 kV if more contrast (and somewhat higher resolution) is desired. The summary here is that in my view a modern TEM is 'biological' when it is used to study biologicals and 'material' when it is used (successfully) to study materials [Acknowledging that field emission, 300kV, and maximally corrected optics are the current norm for the 'best' of the lot tools.].
---------------------------------------------------------------------------------------------
What follows is an extension of the previous 2 paragraphs. You may read on at your own risk.
---------------------------------------------------------------------------------------------
The range of TEM's currently produced by FEI that have the designation 'biological' have the following common specification: higher contrast imaging from specimens with natively low contrast - especially those with no or very little 'staining' (sp., cryo sections). In the FEI BioTwin, contrast is enhanced by lengthening the focal length of the objective lens. Thus, the Twin has approximately 0.2nm and the BioTwin approximately 0.35nm resolution with the most magnanimous specimens.

The easiest way to run a 'materials' TEM as a 'biological' TEM is to run it at 60-80 kV in which range, the electron is longer in wavelength which permits an increase in scattering as well as enhanced phase modulation as it passes thru the specimen(Do I have that right?). So, on to the tool by tool comparison which I do with three URL's to FEI marketing specifics.

Brief comparison between Tecnai 12T G2 (Generation 2 Twin & BioTwin) microscopes

Twin&BioTwin: http://www.fei.com/uploadedFiles/Documents/Content/2006_06_TecnaiG2_.pdf

The Morgagni was designed for the pathology lab and likely does not provide Schertzer (for the materials microscopist) correction. Schertzer's correction is found on the 'materials' TEM where it introduces a standard underfocus (for peak resolution and contrast enhancement)["the imaging of weakly scattering atoms, such as carbon, using an optimal focus [not perfect] which is the focus of minimum contrast], which we call nowadays Scherzer's defocus, should be possible with a resolution limit of 0.2 nm," from Lenzen, et al., Microsc and Microanal(Suppl 3)2003,from Schertzer, 1949).]

Morgagni: http://www.fei.com/products/transmission-electron-microscopes/morgagni.aspx

The Tecnai Osiris is so digital in character that there is no visual observation capability, and it is designed to meet the strict needs of 'structural' biologists! One could consider it a high-end 'biological' TEM.

Osiris: http://www.fei.com/products/transmission-electron-microscopes/tecnai/osiris.aspx

However, these days, a biological TEM could be a Tecnai 12T that is only used to study biological specimens by one person or a BioTwin that is also used for imaging of nanoparticles in the 10-100nm range. On the other hand, the T12 with 3D-Tomography and STEM/EDS could be a 'biological' TEM for one user studying mitochondria and a 'material' for another who is comparing methods by which 1 nm nano crystals can be produced in quantity or studying the distribution of 20-50nm cobalt oxide deposits in silica gel.

Then, there are the High Res TEM's (HRTEM) which are equipped with recently-developed electromagnetic correctors that have changed the language of marketing TEM's from "high resolution" to "resolution at wavelength". These are the FEI Titan and the newer TEAM microscopes. Some of these will be used for studying both 'biological' and 'material' specimens/systems.

If I am ever asked to recommend another TEM purchase for an non-medical academic environment, I will strongly recommend the resolution rather than the contrast, since I know where to find it on the TEM with lower resolution (greater resolving power).

Finally, when I was young, the megavolt TEM was all the rage, and I knew some of the users who used the one in Colorado to study biological material in 1 um (really thick!) sections (e.g., Dr. Lee Peachey (UPenn.edu) who studied the sarcoplasmic reticulum (selectively stained) in 1 um sections and showed beautiful stero pairs when describing his experiences with the TEM that filled a building - and still does.

In contrast, when I received my first dose of training on our T12, I was asked to produce 0.5 um sections of just oxmium-fixed material (I chose testis) and deliver them on grids to the instructor - I remembered when a 400 kV TEM was purchased to permit viewing 0.5 um sections. Without a burp, I had both normal and stereo images of the section. Thus, I was introduced to the difference between the 1980's and 2002. At that moment, the T12 was decidedly the best of the three TEM's I had ever used for biological imaging, yet; I knew that while the manufacturer could 'doctor' any of her/his products to fit a niche in the market place, the best TEM I had ever thought to use was perfect for any biological problem I might wish to tackle with a microscope.

Pre-S 1. Our Quanta 400 tungsten ESEM is eminently 'biological' when I am viewing live (but over-aged) blue cheese at 10-20 torr with a gaseous secondary detector replacing the backscatter detector. It is eminently 'geological' when we are using it (with Oxford INCA Feature automation) and in backscatter mode to identify, count and analyze all the monazites and zircons at 70 Pascals and 20kV, and 120X, on a thin section of rock measuring 20 x 40 mm (in 0.5-150 minutes). It is eminently 'material' when imaging nanometer structures larger than 30-40nm when the signal to noise is sufficient. For smaller objects and details, we have an arrangement with a nearby facility to trade use of our variable pressure SEM for use of their s0-called high resolution SEM (with a field emission gun) with 1nm resolution (and much better signal to noise character).

I hope this helps,

Fred Monson

P.S. Despite appearances, I have no continuing financial arrangements with FEI except transmitting sizeable invoices for service contracts to the Dean and place the majority of my infrequent calls to FEI to the service arm of the company (80% for the more complicated ESEM).

Frederick C. Monson, PhD
Technical Director, CMIRT
West Chester University
West Chester, PA, 19383
610-738-0437
New Web Site: http://cmirt.wcupa.edu/index.html
New Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl





==============================Original Headers==============================
24, 26 -- From FMonson-at-wcupa.edu Wed Dec 16 09:58:47 2009
24, 26 -- Received: from WCU-EX-ET1.PASSHE.LCL (wcu-ex-et1-auth.wcupa.edu [144.26.0.58])
24, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGFwlg1023501
24, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Dec 2009 09:58:47 -0600
24, 26 -- Received: from WCU-EX-HT2.PASSHE.LCL (10.32.4.113) by WCU-EX-ET1.PASSHE.LCL
24, 26 -- (10.32.0.62) with Microsoft SMTP Server (TLS) id 8.2.176.0; Wed, 16 Dec 2009
24, 26 -- 10:58:46 -0500
24, 26 -- Received: from WCU-EX-EMP1-MB.PASSHE.LCL ([fe80::552b:545e:4665:c1fc]) by
24, 26 -- WCU-EX-HT2.PASSHE.LCL ([::1]) with mapi; Wed, 16 Dec 2009 10:58:46 -0500
24, 26 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu}
24, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
24, 26 -- CC: "gw265-at-cam.ac.uk" {gw265-at-cam.ac.uk}
24, 26 -- Date: Wed, 16 Dec 2009 10:58:40 -0500
24, 26 -- Subject: Re: biological TEM vs. materials TEM?
24, 26 -- Thread-Topic: Re: biological TEM vs. materials TEM?
24, 26 -- Thread-Index: Acp+aKLfFzgxxOavSUSkAPJ5AY23eg==
24, 26 -- Message-ID: {86FEE8BCF5652949A08D1EB0545EB42403D7F57882-at-WCU-EX-EMP1-MB.PASSHE.LCL}
24, 26 -- Accept-Language: en-US
24, 26 -- Content-Language: en-US
24, 26 -- X-MS-Has-Attach:
24, 26 -- X-MS-TNEF-Correlator:
24, 26 -- acceptlanguage: en-US
24, 26 -- Content-Type: text/plain; charset="iso-8859-1"
24, 26 -- MIME-Version: 1.0
24, 26 -- Content-Transfer-Encoding: 8bit
24, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGFwlg1023501
==============================End of - Headers==============================




From: weis183-at-yahoo.fr
Date: Wed, 16 Dec 2009 11:12:31 -0600
Subject: [Microscopy] Tr : Re : viaWWW: Rotational average ring pattern

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I did use once a UV lamp to make carbon coated EM grids hydrophilic.
I don't remember the specific wavelength or best range for the job (I don't think it was the far UV) therefore if somebody has some information on this and where to find or how to adapt an existing UV lamp for this purpose, I would be grateful for the information.
cheers,
svetla

Svetla Stoilova-McPhie, PhD
Assistant Professor,
Department of Neuroscience and Cell Biology
Scientist, Sealy Centre for Structural Biology
and Molecular Biophysics
University of Texas Medical Branch at Galveston
301 University Boulevard, Galveston, Texas 77555-0620
Cell: (+1) 281-229-2261
Fax: (1+) 409-747-2200
Email: svmcphie-at-utmb.edu
http://www.utmb.edu/ncb/faculty/Stoilova-McPhie.asp


==============================Original Headers==============================
3, 28 -- From svmcphie-at-utmb.edu Wed Dec 16 10:29:06 2009
3, 28 -- Received: from mx3.utmb.edu (mx3.utmb.edu [129.109.195.8])
3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGGT69k006605
3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 10:29:06 -0600
3, 28 -- Received: from expf1.utmb.edu (expf1.utmb.edu [129.109.116.226])
3, 28 -- by mx3.utmb.edu (8.12.11.20060308/8.12.11) with ESMTP id nBGGStqc013024
3, 28 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO)
3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 10:29:05 -0600
3, 28 -- Received: from EXCHANGE4.utmb.edu ([129.109.116.10]) by expf1.utmb.edu
3, 28 -- ([129.109.116.226]) with mapi; Wed, 16 Dec 2009 10:29:04 -0600
3, 28 -- From: "Stoilova-McPhie, Svetla" {svmcphie-at-utmb.edu}
3, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
3, 28 -- Date: Wed, 16 Dec 2009 10:29:04 -0600
3, 28 -- Subject: UV lamp for making hidrophilic grids
3, 28 -- Thread-Topic: UV lamp for making hidrophilic grids
3, 28 -- Thread-Index: AQHKfmzi+jPcgr7zBUSfpmOyCMneTQ==
3, 28 -- Message-ID: {981C42C3AD3C6D489252BB66CD90374516B9A089B5-at-EXCHANGE4.utmb.edu}
3, 28 -- Accept-Language: en-US
3, 28 -- Content-Language: en-US
3, 28 -- X-MS-Has-Attach:
3, 28 -- X-MS-TNEF-Correlator:
3, 28 -- acceptlanguage: en-US
3, 28 -- Content-Type: text/plain; charset="iso-8859-1"
3, 28 -- MIME-Version: 1.0
3, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.8161:2.4.5,1.2.40,4.0.166 definitions=2009-12-16_05:2009-12-12,2009-12-16,2009-12-16 signatures=0
3, 28 -- X-Proofpoint-Spam-Reason: safe
3, 28 -- Content-Transfer-Encoding: 8bit
3, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGGT69k006605
==============================End of - Headers==============================

From 3dalbert.kwok-at-btcombridge.com Wed Dec 16 11:04:56 2009
Return-Path: {3dalbert.kwok-at-btcombridge.com}
Received: from [83.141.223.53] ([83.141.223.53])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGH4qci022139;
Wed, 16 Dec 2009 11:04:54 -0600
Received: from [7.195.176.37] (account 6.annul-at-opnet.com.tw HELO iwukujkumaq.yiwno.ua)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 791866200 for microscopy-at-microscopy.com; Wed, 16 Dec 2009 18:04:54 +0100

Hello,

You could try Fit2D (http://www.esrf.eu/computing/scientific/FIT2D/)
Not very easy to use but it does the job. It reads Tiff images.

Patrick Weisbecker


________________________________
De : "jwdeng-at-imr.ac.cn" {jwdeng-at-imr.ac.cn}
À : weis183-at-yahoo.fr
Envoyé le : Mer 16 Décembre 2009, 5 h 48 min 24 s
Objet : [Microscopy] viaWWW: Rotational average ring pattern




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please  copy  both jwdeng-at-imr.ac.cn as well as  the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jwdeng-at-imr.ac.cn
Name: J.W. Deng

Title-Subject: [Filtered] Rotational average ring pattern

Question: Dear Listers,
Recently, I want to extract the rotational average intensity
distribution from a ring diffraction pattern. I know there is a
DigitalMicrograph script can do rotational average, however it can't
change the integral angle. So I would like to know what software or
source code can be used to perform the rotational average of an
image. Ii is better if I can change the center and angular range of
the ring pattern, because the beam stop is used usually.

Many Thanks
J.W. Deng

  Login Host: 210.72.130.175
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Dec 15 22:44:44 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBG4ihie023967
6, 11 --     for {microscopy-at-microscopy.com} ; Tue, 15 Dec 2009 22:44:43 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240802c74e16297f69-at-[206.69.208.22]}
6, 11 -- Date: Tue, 15 Dec 2009 22:44:41 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: jwdeng-at-imr.ac.cn (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Rotational average ring pattern
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================






==============================Original Headers==============================
20, 28 -- From weis183-at-yahoo.fr Wed Dec 16 11:12:31 2009
20, 28 -- Received: from n22.bullet.mail.ukl.yahoo.com (n22.bullet.mail.ukl.yahoo.com [87.248.110.139])
20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBGHCUkW022472
20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 11:12:31 -0600
20, 28 -- Received: from [217.12.4.215] by n22.bullet.mail.ukl.yahoo.com with NNFMP; 16 Dec 2009 17:12:30 -0000
20, 28 -- Received: from [87.248.111.149] by t2.bullet.ukl.yahoo.com with NNFMP; 16 Dec 2009 17:12:30 -0000
20, 28 -- Received: from [127.0.0.1] by omp206.mail.ukl.yahoo.com with NNFMP; 16 Dec 2009 17:12:30 -0000
20, 28 -- X-Yahoo-Newman-Property: ymail-3
20, 28 -- X-Yahoo-Newman-Id: 179424.52399.bm-at-omp206.mail.ukl.yahoo.com
20, 28 -- Received: (qmail 52744 invoked by uid 60001); 16 Dec 2009 17:12:30 -0000
20, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1260983550; bh=NtPKSYF6b7IOUvwQ3E1Pl3ADn9KgBp0EkLVy5lYFjw8=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding; b=EMDlS6Ix8VHuoCduquKjIiNA9OhwAs8a0JFji2x4qhbk1b3botyEGzzpoqpeSPvYCHVGtiU2EkS8IpJ8zcHeSTbD+/0vKnm+2TnaPs2vRXsrVFSCDmBvQDVcgjBVaLC36R/CU794+vOfuGxGfUXckqRyng585aPU3Kj27zVPmU0=
20, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
20, 28 -- s=s1024; d=yahoo.fr;
20, 28 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding;
20, 28 -- b=DUw4sW54HCWrzWVGxV4E/YuHxTb3fTClNg0XGpC5HXjn+/6R0BbO9XPH5ujdg1F+sCADPODfpYHA2RFPQBiCZFP61aLpdLM14PgnwpCSXUcGTwap1fufPz7UqqTK9MOiyJGc6pRlbjuY/oxexx+kC9mcQ8x2Dxd3lJUQmDTqeyc=;
20, 28 -- Message-ID: {3281.52701.qm-at-web24606.mail.ird.yahoo.com}
20, 28 -- X-YMail-OSG: BzFgWUcVM1nnbV5THlY44x6MmPphnQFWHaBF8LboAwEl0rldHtNIJVgDUxNr6tWWOkzzA9HtN0fYW4I1eQpfMcE8NhRG_mqOEa1iKWKhy8Wxh.1Wh2wiV3HXD8WpIc76dFOP_2lBnodqALAgCMunvVLNeN07mVn5bRppteC19tLEmt19D1ZZOTUjHFY2JOvjysDMvPQUYWlnG3b.jwhoxOELJdhKH3eyHK7xFc4y6F30s06Pkawv9sldNdQ3XpbbbMaSa9Er3d4lBvdT63TvcGz.Jv6kCYe08uzotw--
20, 28 -- Received: from [147.210.80.23] by web24606.mail.ird.yahoo.com via HTTP; Wed, 16 Dec 2009 17:12:29 GMT
20, 28 -- X-Mailer: YahooMailRC/240.3 YahooMailWebService/0.8.100.260964
20, 28 -- Date: Wed, 16 Dec 2009 17:12:29 +0000 (GMT)
20, 28 -- From: Patrick Weisbecker {weis183-at-yahoo.fr}
20, 28 -- Subject: Tr : Re : [Microscopy] viaWWW: Rotational average ring pattern
20, 28 -- To: jwdeng-at-imr.ac.cn
20, 28 -- Cc: microscopy-at-microscopy.com
20, 28 -- MIME-Version: 1.0
20, 28 -- Content-Type: text/plain; charset=iso-8859-1
20, 28 -- Content-Transfer-Encoding: 8bit
20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGHCUkW022472
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 16 Dec 2009 11:15:35 -0600
Subject: [Microscopy] Digital Micrograph image conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collective,

We are using Digital Micrograph Version 1.81.78 on our TEM and often have an issue when converting the native .dm3 files into .tif format. The image often (not always) loses a great deal of contrast compared to the original capture and we can't seem to get good control of this with the Brightness/Contrast/Gamma controls in the DM program itself before saving the image.

We can adjust the image using Levels in Photoshop, but it seems like we're dumping part of the grayscale range during the 32-bit .dm3 to 8-bit .tif conversion. Also, some clients are unhappy about having to play around with large numbers of images. They want 'em user ready right off the scope, reasonable or not.

Does anyone else have this issue and a possible cure? Are we doing something dumb?

Cheers and thanks,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
9, 30 -- From TindallR-at-missouri.edu Wed Dec 16 11:15:35 2009
9, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53])
9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGHFYBF027265
9, 30 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 11:15:34 -0600
9, 30 -- X-IronPort-Anti-Spam-Filtered: true
9, 30 -- X-IronPort-Anti-Spam-Result: ArkEACekKEvRauUo/2dsb2JhbACcNaxtAQmFKIhIgkiBYwQ
9, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
9, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Dec 2009 11:15:34 -0600
9, 30 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 30 -- Wed, 16 Dec 2009 11:15:34 -0600
9, 30 -- Received: from um-email06.um.umsystem.edu ([169.254.1.52]) by
9, 30 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Wed, 16 Dec 2009
9, 30 -- 11:15:33 -0600
9, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
9, 30 -- Date: Wed, 16 Dec 2009 11:15:32 -0600
9, 30 -- Subject: Digital Micrograph image conversion
9, 30 -- Thread-Topic: Digital Micrograph image conversion
9, 30 -- Thread-Index: Acp+c1+SReZXVzWWRd6+jV5TLktQ9w==
9, 30 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129C99FDFE-at-UM-EMAIL06.um.umsystem.edu}
9, 30 -- Accept-Language: en-US
9, 30 -- Content-Language: en-US
9, 30 -- X-MS-Has-Attach:
9, 30 -- X-MS-TNEF-Correlator:
9, 30 -- acceptlanguage: en-US
9, 30 -- Content-Type: text/plain; charset="us-ascii"
9, 30 -- MIME-Version: 1.0
9, 30 -- X-OriginalArrivalTime: 16 Dec 2009 17:15:34.0077 (UTC) FILETIME=[609DBED0:01CA7E73]
9, 30 -- Content-Transfer-Encoding: 8bit
9, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGHFYBF027265
==============================End of - Headers==============================




From: rharmon-at-gatan.com
Date: Wed, 16 Dec 2009 12:40:25 -0600
Subject: [Microscopy] Re: Digital Micrograph image conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Randy,

I noticed your query concerning TIF saving from DigitalMicrograph and did a
quick test using GMS 1.81.78 to see if there were any obvious issues. I
tried saving the display as grayscale, with the annotations burned in at the
actual resolution of the DM image. I tried with the default
brightness/contrast/gamma and with an adjusted one. The images I saved
opened in PhotoShop Elements and looked identical to the originals displayed
in DM.

You mentioned that the problem did not always occur. Could you contact me
directly and provide some examples where the issue has arisen to help
clarify what is going on?

Best Regards,
Robin Harmon
Software Program Manager
Gatan Inc.




==============================Original Headers==============================
7, 20 -- From rharmon-at-gatan.com Wed Dec 16 12:40:24 2009
7, 20 -- Received: from smtp.gatan.com (smtp.gatan.com [209.3.42.249])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGIeOF8022024
7, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 12:40:24 -0600
7, 20 -- Received: from rharmon ([10.13.7.66]) by smtp.gatan.com with Microsoft SMTPSVC(5.0.2195.6713);
7, 20 -- Wed, 16 Dec 2009 10:44:00 -0800
7, 20 -- From: "Robin Harmon" {rharmon-at-gatan.com}
7, 20 -- To: {Microscopy-at-microscopy.com}
7, 20 -- Cc: {tindallr-at-missouri.edu}
7, 20 -- Subject: Re: Digital Micrograph image conversion
7, 20 -- Date: Wed, 16 Dec 2009 10:40:19 -0800
7, 20 -- Message-ID: {009801ca7e7f$38177c10$a8467430$-at-com}
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain;
7, 20 -- charset="us-ascii"
7, 20 -- Content-Transfer-Encoding: 7bit
7, 20 -- X-Mailer: Microsoft Office Outlook 12.0
7, 20 -- thread-index: Acp+fzfi8M1nWj9wRyuMqBIl3PcF8A==
7, 20 -- Content-Language: en-us
7, 20 -- X-OriginalArrivalTime: 16 Dec 2009 18:44:00.0236 (UTC) FILETIME=[BB555AC0:01CA7E7F]
==============================End of - Headers==============================




From: bdg160-at-psu.edu
Date: Wed, 16 Dec 2009 12:58:15 -0600
Subject: [Microscopy] Re : Rotational average ring pattern

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I have found the plugin "Radial Profile Plot Extended" for ImageJ to
be the best for this. You can find it here:
http://rsb.info.nih.gov/ij/plugins/ . If you don't already have
imageJ installed you'll have to do that to. It allows you to select
the center (X,Y) coordinates and the angle over which to integrate.
It will then display the profile for you and you can export that data
to a .txt file to plot in your favorite plotting package.

-Bryan


--
Bryan D. Gauntt

3M Graduate Fellow
Department of Materials Science and Engineering
274 Materials Research Lab
Penn State University
(814) 574-2751

==============================Original Headers==============================
5, 32 -- From bdgauntt-at-gmail.com Wed Dec 16 12:58:15 2009
5, 32 -- Received: from mail-fx0-f217.google.com (mail-fx0-f217.google.com [209.85.220.217])
5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGIwESB004579
5, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 12:58:15 -0600
5, 32 -- Received: by fxm9 with SMTP id 9so1267213fxm.10
5, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 10:58:14 -0800 (PST)
5, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
5, 32 -- d=gmail.com; s=gamma;
5, 32 -- h=domainkey-signature:mime-version:sender:received:date
5, 32 -- :x-google-sender-auth:message-id:subject:from:to:content-type;
5, 32 -- bh=1TnRDL6Njs2CYTRiK/wSWQsuMr+d8IfMa3M0/0128Mw=;
5, 32 -- b=MQjxznzDfEFM667J7p91+XYiv6sgBbR3PCNSRhgA/URzxny/11HYt0Zz6c62WzV66a
5, 32 -- IADCUZpw8D5VgyWS6CEkOh10Heg9bXTD/jVHChWJ+WRigb7yP67h+WpIOk1kUgWev/Ht
5, 32 -- OpSS/vKRh34xuk5SBCQv0iy7F1OERVR4r+SLI=
5, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws;
5, 32 -- d=gmail.com; s=gamma;
5, 32 -- h=mime-version:sender:date:x-google-sender-auth:message-id:subject
5, 32 -- :from:to:content-type;
5, 32 -- b=lzBdOlSwSMdMsmagKwvlsi7yZMPlwsJrSWWiNHq/GxBHYNRsSWb31r/DzrBGZoOe8p
5, 32 -- i6bCB/7KlVgZIKrgsIAUlj0epGdIQTuklY7/uxhs+GHHEZMU2330fCqLOa3AqFfg9o/3
5, 32 -- f2vCDPArO5o2Cplpy3JavNriqTsbERGVpXeQU=
5, 32 -- MIME-Version: 1.0
5, 32 -- Sender: bdgauntt-at-gmail.com
5, 32 -- Received: by 10.216.93.66 with SMTP id k44mr506657wef.67.1260989798368; Wed,
5, 32 -- 16 Dec 2009 10:56:38 -0800 (PST)
5, 32 -- Date: Wed, 16 Dec 2009 13:56:38 -0500
5, 32 -- X-Google-Sender-Auth: 9f92ea1e469955e9
5, 32 -- Message-ID: {f4075d260912161056n1326c678j7ff79b9302973393-at-mail.gmail.com}
5, 32 -- Subject: Re : Rotational average ring pattern
5, 32 -- From: Bryan Gauntt {bdg160-at-psu.edu}
5, 32 -- To: Microscopy {Microscopy-at-microscopy.com}
5, 32 -- Content-Type: text/plain; charset=ISO-8859-1
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Wed, 16 Dec 2009 13:41:12 -0600
Subject: [Microscopy] Re: Digital Micrograph image conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have perhaps the same issue occasionally when converting DM3 to tif. We
generally use batch convert, and sometimes one or more images in the batch
appear solid black when opened (can see image if bright/contrast pushed
toward limits). Converting the same batch a second time usually results in
all images being OK. It's simple, but seems to take care of what may be
random glitch in the code.

Cheers


Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5453
fax: 860-486-4745




==============================Original Headers==============================
6, 22 -- From raristau-at-ims.uconn.edu Wed Dec 16 13:41:12 2009
6, 22 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGJfCsu020151
6, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Dec 2009 13:41:12 -0600
6, 22 -- Received: from [137.99.20.157] (d20h157.public.uconn.edu [137.99.20.157])
6, 22 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id nBGJf8m4016807
6, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Dec 2009 14:41:08 -0500
6, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122
6, 22 -- Date: Wed, 16 Dec 2009 14:40:59 -0500
6, 22 -- Subject: Re: Digital Micrograph image conversion
6, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
6, 22 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
6, 22 -- Message-ID: {C74EA1FB.ABD%raristau-at-ims.uconn.edu}
6, 22 -- Thread-Topic: Digital Micrograph image conversion
6, 22 -- Thread-Index: Acp+h7ET72CLP+p6Ed6nTAAbY55CVA==
6, 22 -- Mime-version: 1.0
6, 22 -- Content-type: text/plain;
6, 22 -- charset="US-ASCII"
6, 22 -- Content-transfer-encoding: 7bit
6, 22 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
6, 22 -- X-UConn-MailScanner: Found to be clean
6, 22 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: rharmon-at-gatan.com
Date: Wed, 16 Dec 2009 14:46:42 -0600
Subject: [Microscopy] Re: DigitalMicrograph image conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roger,

The occasional image being black when you convert a batch of dm3 files to
TIF is indeed a bug that was fixed for GMS 1.8.3. Depending on your hardware
configuration you may be able to upgrade to this release with no problems.
Contact me directly if you are interested.

Regards,
Robin Harmon
Software Program Manager
Gatan Inc.



==============================Original Headers==============================
5, 19 -- From rharmon-at-gatan.com Wed Dec 16 14:46:41 2009
5, 19 -- Received: from smtp.gatan.com (smtp.gatan.com [209.3.42.249])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGKke3c011575
5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 14:46:40 -0600
5, 19 -- Received: from rharmon ([10.13.7.66]) by smtp.gatan.com with Microsoft SMTPSVC(5.0.2195.6713);
5, 19 -- Wed, 16 Dec 2009 12:50:17 -0800
5, 19 -- From: "Robin Harmon" {rharmon-at-gatan.com}
5, 19 -- To: {Microscopy-at-microscopy.com}
5, 19 -- Subject: Re: DigitalMicrograph image conversion
5, 19 -- Date: Wed, 16 Dec 2009 12:46:36 -0800
5, 19 -- Message-ID: {00d501ca7e90$dc4796b0$94d6c410$-at-com}
5, 19 -- MIME-Version: 1.0
5, 19 -- Content-Type: text/plain;
5, 19 -- charset="us-ascii"
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- X-Mailer: Microsoft Office Outlook 12.0
5, 19 -- thread-index: Acp+kNwQRi5slLO9QuSGZECFpPljWg==
5, 19 -- Content-Language: en-us
5, 19 -- X-OriginalArrivalTime: 16 Dec 2009 20:50:17.0251 (UTC) FILETIME=[5F961730:01CA7E91]
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 16 Dec 2009 14:56:46 -0600
Subject: [Microscopy] Re:ImageJ - DigitalMicrograph image conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

You can use ImageJ which reads the full 16bit raw
DM3 file format. I do this routinely and don't loose any
information. You then have all the ImageJ tools to
manipulate the data.

ImageJ can be downloaded for free here:

http://rsbweb.nih.gov/ij/

Nestor
Your Friendly Neighborhood SysOp
--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679), Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec-at-ANL
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Fellow of the Microscopy Society of America
Visiting Professor of Physics - Northern Illinois University





===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
16, 24 -- From zaluzec-at-aaem.amc.anl.gov Wed Dec 16 14:56:46 2009
16, 24 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGKukAk028374
16, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 14:56:46 -0600
16, 24 -- Received: from localhost (localhost [127.0.0.1])
16, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTP id 1EDF3A46CF
16, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 14:56:46 -0600 (CST)
16, 24 -- X-Virus-Scanned: amavisd-new at localhost.localnet
16, 24 -- Received: from aaem.amc.anl.gov ([127.0.0.1])
16, 24 -- by localhost (aem005.amc.anl.gov [127.0.0.1]) (amavisd-new, port 10024)
16, 24 -- with ESMTP id iTseUh+RIJAm for {microscopy-at-microscopy.com} ;
16, 24 -- Wed, 16 Dec 2009 14:56:45 -0600 (CST)
16, 24 -- Received: from [146.139.72.108] (aem108.amc.anl.gov [146.139.72.108])
16, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTPA id 80227A46BF
16, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 14:56:45 -0600 (CST)
16, 24 -- Mime-Version: 1.0
16, 24 -- Message-Id: {p06240810c74ef900b7f8-at-[146.139.72.108]}
16, 24 -- In-Reply-To: {200912162046.nBGKkgut011587-at-ns.microscopy.com}
16, 24 -- References: {200912162046.nBGKkgut011587-at-ns.microscopy.com}
16, 24 -- Date: Wed, 16 Dec 2009 14:56:44 -0600
16, 24 -- To: microscopy-at-microscopy.com
16, 24 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
16, 24 -- Subject: Re:ImageJ - DigitalMicrograph image conversion
16, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Wed, 16 Dec 2009 15:34:57 -0600
Subject: [Microscopy] Digital Micrograph Screenshot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have placed a screenshot of the problem I described at http://www.emc.missouri.edu/lookatthis.htm, just in case people are wondering what we're talking about. The image on the left is the original .dm3 test file as viewed in ImageJ. The image on the right is the corresponding .tif file as converted by Digital Micrograph using their Batch Convert function, also viewed in ImageJ. Similar results show up in Photoshop.

The .dm3 and .tif files show up as pretty much identical IF you only view them in Digital Micrograph apparently. In other words, if you batch convert the .dm3s within DM, then open up the resulting .tifs or .jpgs, they will both look fine. Switching between imaging platforms seems to cause the problem, but hardly nobody here uses DM to do image processing on their home or office computers after they leave our lab, so they usually see the pasty files.

I agree with Nestor that ImageJ works well with these files, if you open the .dm3 in ImageJ and save it as a .tif from ImageJ, which preserves the 32-bit indexed color format. And, as John Russ points out, the newer Photoshop versions will handle the high-bit depth images, however I'm pretty sure there is no Photoshop plugin as yet for opening .dm3 files directly. Please correct me if I'm wrong.

Cheers,
Randy


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original Headers==============================
10, 30 -- From TindallR-at-missouri.edu Wed Dec 16 15:34:57 2009
10, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGLYuF4019299
10, 30 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 15:34:56 -0600
10, 30 -- X-IronPort-Anti-Spam-Filtered: true
10, 30 -- X-IronPort-Anti-Spam-Result: An8FACbhKEvRauUo/2dsb2JhbACcPat7AQmFLYhIgkiBYwQ
10, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40])
10, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 16 Dec 2009 15:34:56 -0600
10, 30 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 30 -- Wed, 16 Dec 2009 15:34:56 -0600
10, 30 -- Received: from um-email06.um.umsystem.edu ([169.254.1.52]) by
10, 30 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed, 16 Dec 2009
10, 30 -- 15:34:56 -0600
10, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
10, 30 -- Date: Wed, 16 Dec 2009 15:34:54 -0600
10, 30 -- Subject: Digital Micrograph Screenshot
10, 30 -- Thread-Topic: Digital Micrograph Screenshot
10, 30 -- Thread-Index: Acp+l5si4aFA4hePSU+MahLkS+cEdQ==
10, 30 -- Message-ID: {9422E68616A7C648A281C0B5CD22A4B8129C99FE81-at-UM-EMAIL06.um.umsystem.edu}
10, 30 -- Accept-Language: en-US
10, 30 -- Content-Language: en-US
10, 30 -- X-MS-Has-Attach:
10, 30 -- X-MS-TNEF-Correlator:
10, 30 -- acceptlanguage: en-US
10, 30 -- Content-Type: text/plain; charset="us-ascii"
10, 30 -- MIME-Version: 1.0
10, 30 -- X-OriginalArrivalTime: 16 Dec 2009 21:34:56.0354 (UTC) FILETIME=[9C74C420:01CA7E97]
10, 30 -- Content-Transfer-Encoding: 8bit
10, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGLYuF4019299
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 16 Dec 2009 18:06:16 -0600
Subject: [Microscopy] Digital Micrograph Screenshot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't use ImageJ very much and I definitely don't use Digital
Micrograph, but I am confused.

The TIF file and its histogram within ImageJ look consistent. The
histogram is definitely weighted to the right side, i.e., the light
grays. The displayed image is consistent with that. It has no dark
grays.

The DM3 image and its histogram are NOT consistent. The image seems to
span the whole gray scale range. Some areas are nearly black and some
are nearly washed out. However, the histogram shows only about one
fourth of the gray levels to be occupied. The lightest pixel should be a
middle gray and the darkest pixel should be a dark gray. That doesn't
add up.

Perhaps there is something in ImageJ to autoscale images to spread the
histogram to fill the available grayscale display. That seems to have
been done for the DM3 image, but not with the TIF image. Maybe it is
only done with deeper images (i.e., more than 8 bits per pixel).

It also looks like the converted image was prepared by scaling the grays
from zero to the maximum brightness. They do NOT seem to start scaling
from the minimum brightness. Therefore, the histogram seems to have
shifted over to the right. If the original image would have had a darker
pixel to begin with, the effect would have been less obvious.
That seems to be the source of the problem.

I wonder if this traces back to more automatic functions being used to
collect the images. I am not familiar with TEMs. I know that I would
forego the automatic brightness function with SEMs and set my brightness
range manually to fill the available gray scale (input range to the
A/D).

I will also ask why so many gray levels are used on this sample? It
looks like many a given phase would be spread across many gray levels
even at 256 levels. The extra 24 bits of data per pixel seem to be
wasted in this case. I suppose other images benefit from the greater
data depth.

I could also wonder aloud why indexed color was used instead of gray
scale interpretation? I suppose there is a reason that may have much to
do with manipulating brightness contrast and gamma without changing the
actual data. I am just curious.

Hoping this helps,
Warren

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Wednesday, December 16, 2009 3:35 PM
To: wesaia-at-iastate.edu

Hi all,

I have placed a screenshot of the problem I described at
http://www.emc.missouri.edu/lookatthis.htm, just in case people are
wondering what we're talking about. The image on the left is the
original .dm3 test file as viewed in ImageJ. The image on the right is
the corresponding .tif file as converted by Digital Micrograph using
their Batch Convert function, also viewed in ImageJ. Similar results
show up in Photoshop.

The .dm3 and .tif files show up as pretty much identical IF you only
view them in Digital Micrograph apparently. In other words, if you
batch convert the .dm3s within DM, then open up the resulting .tifs or
.jpgs, they will both look fine. Switching between imaging platforms
seems to cause the problem, but hardly nobody here uses DM to do image
processing on their home or office computers after they leave our lab,
so they usually see the pasty files.

I agree with Nestor that ImageJ works well with these files, if you open
the .dm3 in ImageJ and save it as a .tif from ImageJ, which preserves
the 32-bit indexed color format. And, as John Russ points out, the
newer Photoshop versions will handle the high-bit depth images, however
I'm pretty sure there is no Photoshop plugin as yet for opening .dm3
files directly. Please correct me if I'm wrong.

Cheers,
Randy


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan
Sons of Norway: http://www.sofn.com




==============================Original
Headers==============================
10, 30 -- From TindallR-at-missouri.edu Wed Dec 16 15:34:57 2009
10, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu
(mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id nBGLYuF4019299
10, 30 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009
15:34:56 -0600
10, 30 -- X-IronPort-Anti-Spam-Filtered: true
10, 30 -- X-IronPort-Anti-Spam-Result:
An8FACbhKEvRauUo/2dsb2JhbACcPat7AQmFLYhIgkiBYwQ
10, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu)
([209.106.229.40])
10, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 16 Dec
2009 15:34:56 -0600
10, 30 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182])
by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
10, 30 -- Wed, 16 Dec 2009 15:34:56 -0600
10, 30 -- Received: from um-email06.um.umsystem.edu ([169.254.1.52]) by
10, 30 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed,
16 Dec 2009
10, 30 -- 15:34:56 -0600
10, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
10, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
10, 30 -- Date: Wed, 16 Dec 2009 15:34:54 -0600
10, 30 -- Subject: Digital Micrograph Screenshot
10, 30 -- Thread-Topic: Digital Micrograph Screenshot
10, 30 -- Thread-Index: Acp+l5si4aFA4hePSU+MahLkS+cEdQ==
10, 30 -- Message-ID:
{9422E68616A7C648A281C0B5CD22A4B8129C99FE81-at-UM-EMAIL06.um.umsystem.edu}
10, 30 -- Accept-Language: en-US
10, 30 -- Content-Language: en-US
10, 30 -- X-MS-Has-Attach:
10, 30 -- X-MS-TNEF-Correlator:
10, 30 -- acceptlanguage: en-US
10, 30 -- Content-Type: text/plain; charset="us-ascii"
10, 30 -- MIME-Version: 1.0
10, 30 -- X-OriginalArrivalTime: 16 Dec 2009 21:34:56.0354 (UTC)
FILETIME=[9C74C420:01CA7E97]
10, 30 -- Content-Transfer-Encoding: 8bit
10, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id nBGLYuF4019299
==============================End of -
Headers==============================


==============================Original Headers==============================
25, 36 -- From wesaia-at-iastate.edu Wed Dec 16 17:21:08 2009
25, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15])
25, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGNL7lc014322
25, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 17:21:07 -0600
25, 36 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47])
25, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id nBGNL6fY011850
25, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 17:21:06 -0600
25, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp
25, 36 -- id 3ca7_b0495920_ea99_11de_b7dc_00137253420a;
25, 36 -- Wed, 16 Dec 2009 17:21:07 -0600
25, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85])
25, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id nBGNL6BM004304
25, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 17:21:06 -0600
25, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959);
25, 36 -- Wed, 16 Dec 2009 17:21:07 -0600
25, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
25, 36 -- Content-class: urn:content-classes:message
25, 36 -- MIME-Version: 1.0
25, 36 -- Content-Type: text/plain;
25, 36 -- charset="us-ascii"
25, 36 -- Subject: RE: [Microscopy] Digital Micrograph Screenshot
25, 36 -- Date: Wed, 16 Dec 2009 17:23:01 -0600
25, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D727046CD80B-at-maire.eng.iastate.edu}
25, 36 -- In-Reply-To: {200912162135.nBGLZTqQ020406-at-ns.microscopy.com}
25, 36 -- X-MS-Has-Attach:
25, 36 -- X-MS-TNEF-Correlator:
25, 36 -- Thread-Topic: [Microscopy] Digital Micrograph Screenshot
25, 36 -- Thread-Index: Acp+l7Enw3N1DXbBSJq3yV78o72uEAADJwNw
25, 36 -- References: {200912162135.nBGLZTqQ020406-at-ns.microscopy.com}
25, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu}
25, 36 -- To: {Microscopy-at-microscopy.com}
25, 36 -- X-OriginalArrivalTime: 16 Dec 2009 23:21:07.0154 (UTC) FILETIME=[71BFB320:01CA7EA6]
25, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.12.16.230928
25, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0'
25, 36 -- Content-Transfer-Encoding: 8bit
25, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBGNL7lc014322
==============================End of - Headers==============================

From a1gkant_wu-at-audix.com.cn Wed Dec 16 17:56:42 2009
Return-Path: {a1gkant_wu-at-audix.com.cn}
Received: from servidor.UMmotos (corporat190-025249082.sta.etb.net.co [190.25.249.82] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBGNufi2029410;
Wed, 16 Dec 2009 17:56:41 -0600
Received: from [157.33.57.28] (helo=bibriiva.jmmcuk.net)
by servidor.UMmotos with esmtpa (Exim 4.69)
(envelope-from )
id 1MML9A-2916ob-9K
for microscopy-at-microscopy.com; Wed, 16 Dec 2009 18:56:40 -0500

Interesting thread. Indexed color catches my attention.
Many of my imaging programs don't like indexed color and
render them black. The SEM and EDS images are indexed
color. I convert their TIFFs to greyscale using Mode in
Photoshop and move on.

Perhaps ImageJ Type/8-bit would do this function?

gary g.



At 03:23 PM 12/16/2009, you wrote:
[snip]


} I could also wonder aloud why indexed color was used instead of gray
} scale interpretation? I suppose there is a reason that may have much to
} do with manipulating brightness contrast and gamma without changing the
} actual data. I am just curious.
}
} Hoping this helps,
} Warren


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Wed Dec 16 18:06:16 2009
9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id nBH06GSj029572
9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2009 18:06:16 -0600
9, 20 -- Message-Id: {200912170006.nBH06GSj029572-at-ns.microscopy.com}
9, 20 -- Received: (qmail 31350 invoked from network); 16 Dec 2009 15:42:55 -0800
9, 20 -- Received: by simscan 1.1.0 ppid: 31340, pid: 31346, t: 0.1489s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211)
9, 20 -- by smtp2 with SMTP; 16 Dec 2009 15:42:55 -0800
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
9, 20 -- Date: Wed, 16 Dec 2009 16:06:14 -0800
9, 20 -- To: wesaia-at-iastate.edu
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] RE: Digital Micrograph Screenshot
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200912162323.nBGNNXWs016609-at-ns.microscopy.com}
9, 20 -- References: {200912162323.nBGNNXWs016609-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: smithj-at-winthrop.edu
Date: Thu, 17 Dec 2009 09:04:29 -0600
Subject: [Microscopy] service for Leica 1512 in the southeast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a Leica 1512 paraffin microtome that's not advancing reliably
(varying section thickness). Anyone service these on-site in the
Southeast US?
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)


==============================Original Headers==============================
4, 18 -- From smithj-at-winthrop.edu Thu Dec 17 09:04:29 2009
4, 18 -- Received: from class.winthrop.edu (class.winthrop.edu [199.79.254.233])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBHF4TG0012359
4, 18 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2009 09:04:29 -0600
4, 18 -- Received: from berlin.win.winthrop.edu [10.2.0.22] by class.winthrop.edu with ESMTP
4, 18 -- (SMTPD32-8.14) id A883874902E4; Thu, 17 Dec 2009 10:04:35 -0500
4, 18 -- Received: from Julian-Smiths-Computer.local ([10.3.80.180]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959);
4, 18 -- Thu, 17 Dec 2009 10:04:35 -0500
4, 18 -- Message-ID: {4B2A487C.1040508-at-winthrop.edu}
4, 18 -- Date: Thu, 17 Dec 2009 10:04:28 -0500
4, 18 -- From: Julian Smith III {smithj-at-winthrop.edu}
4, 18 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812)
4, 18 -- MIME-Version: 1.0
4, 18 -- To: microscopy-at-microscopy.com
4, 18 -- Subject: service for Leica 1512 in the southeast?
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
4, 18 -- X-OriginalArrivalTime: 17 Dec 2009 15:04:35.0035 (UTC) FILETIME=[3EACDAB0:01CA7F2A]
==============================End of - Headers==============================




From: rharmon-at-gatan.com
Date: Thu, 17 Dec 2009 12:27:17 -0600
Subject: [Microscopy] Re: DigitalMicrograph Screenshot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Warren,

Perhaps I can clarify.

Both ImageJ and DigitalMicrograph perform an intensity transform to map from
the raw data to the display on the screen. This consists of a survey to
locate data values to be assigned to black and white and, potentially, a
brightness/contrast/gamma adjustment. This why ImageJ displays the DM3 image
with shades spanning the range from black to white yet its histogram
indicates that the data lie in about one quarter of the histogram range.
This is a common process in image display applications.

The converted image looks pale because it was generated from a display with
a gamma of 0.6 and this skewed the pixels in the display towards white. The
histogram of this display therefore shows pixels skewed to the right!
Without the gamma correction the first peak of the histogram lies at a pixel
value of around 90 rather than the 150 you can see in this case.

Regarding the large number of grey levels, I think the CCD detector in the
camera used had a dynamic range of 16 bits. After dark subtracting and gain
normalizing the data there are negative pixel values so the data do not lie
within the range of a 16-bit unsigned or signed integer. We therefore chose
to put it in a 32-bit signed integer. You are right that many of the 32 bits
are not used but we prefer to maintain the integrity of the data rather than
clip or offset it to fit in a 16-bit data type.

Best Regards,
Robin Harmon
Software Program Manager
Gatan Inc.




==============================Original Headers==============================
9, 19 -- From rharmon-at-gatan.com Thu Dec 17 12:27:17 2009
9, 19 -- Received: from smtp.gatan.com (smtp.gatan.com [209.3.42.249])
9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBHIRGFs007865
9, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2009 12:27:17 -0600
9, 19 -- Received: from rharmon ([10.13.7.66]) by smtp.gatan.com with Microsoft SMTPSVC(5.0.2195.6713);
9, 19 -- Thu, 17 Dec 2009 10:30:53 -0800
9, 19 -- From: "Robin Harmon" {rharmon-at-gatan.com}
9, 19 -- To: {Microscopy-at-microscopy.com}
9, 19 -- Subject: Re: DigitalMicrograph Screenshot
9, 19 -- Date: Thu, 17 Dec 2009 10:27:13 -0800
9, 19 -- Message-ID: {013301ca7f46$8d8851c0$a898f540$-at-com}
9, 19 -- MIME-Version: 1.0
9, 19 -- Content-Type: text/plain;
9, 19 -- charset="us-ascii"
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- X-Mailer: Microsoft Office Outlook 12.0
9, 19 -- thread-index: Acp/Ro0YnfL/Hr6cTz+4q3lOlRGxdQ==
9, 19 -- Content-Language: en-us
9, 19 -- X-OriginalArrivalTime: 17 Dec 2009 18:30:53.0332 (UTC) FILETIME=[10B71540:01CA7F47]
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Thu, 17 Dec 2009 14:29:37 -0600
Subject: [Microscopy] SPM/STEM Postdoctoral position open - Local Electrochemical Processes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Postdoctoral Research Associate in Local Bias-Induced Transitions and
Electrochemical Processes in Energy Storage Materials, Materials Science
and Technology Division, Oak Ridge National Laboratory, ORNL10-48-MSTD

Project Description:

The Materials Science and Technology Division at Oak Ridge National
Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position
in the field of scanning probe microscopy (SPM) and combined SPM -
high-resolution scanning transmission electron microscopy (STEM) of
oxide energy storage and generation materials. The position is available
starting February 1, 2010 and renewable up to the total of 3 years. This
program takes advantage of ORNL’s suite of advanced electron
microscopes, including 5 aberration-corrected instruments, and SPM
capabilities including energy dissipation imaging and Li-ion microscopy
developed at the Center for Nanophase Materials Sciences.

The successful applicant must demonstrate knowledge of general aspects
of solid-state electrochemistry and physics and chemistry of
perovskites. The applicants with background in STEM-EELS and working
knowledge of SPM methods, or in-depth background in SPM of ferroelectric
and energy storage materials are welcome to apply. This position
provides an opportunity to join an experienced team working in a highly
collaborative environment on the projects ranging from heterogeneous
catalysis to oxide materials to semiconductors. Interactions with
theoretical researchers are anticipated on a daily basis.

Qualifications: This position requires a Ph.D. in materials science,
physics, or related field, with an emphasis on solid-state
electrochemistry, SPM, or STEM/EELS. Knowledge of MatLab is a plus.
Excellent oral and written communication skills are required, and
presentations and publication of scientific results in peer-reviewed
journals are expected. The applicant must have the ability to work in a
team and interact effectively with a broad range of colleagues.
Applicants cannot have received the most recent degree more than five
years prior to the date of application and must complete all degree
requirements before starting their appointment. Applications will be
accepted until the position is filled.

Technical Questions: Questions regarding the position can be directed to
Drs. Albina Y. Borisevich, albinab-at-ornl.gov, Stephen J. Pennycook,
pennycooksj-at-ornl.gov, and Sergei V. Kalinin, sergei2-at-ornl.gov. Please
include the requisition number and title when corresponding.

--
Sergei V. Kalinin
co-Theme Leader for Functional Imaging on the Nanoscale
The Center for Nanophase Materials Sciences
and Materials Sciences and Technology Division
Oak Ridge National Laboratory
Oak Ridge, TN 37922

Adjunct Associate Professor,
Department of Materials Science and Engineering,
University of Tennessee, Knoxville

Phone: (865) 241-0236
http://imaging.ornl.gov


==============================Original Headers==============================
10, 25 -- From sergei2-at-ornl.gov Thu Dec 17 14:29:37 2009
10, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBHKTbqp026851
10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2009 14:29:37 -0600
10, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1])
10, 25 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
10, 25 -- with ESMTP id {0KUT00F0QCXCTH-at-emroute1.ornl.gov} for
10, 25 -- microscopy-at-microscopy.com; Thu, 17 Dec 2009 15:29:36 -0500 (EST)
10, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov
10, 25 -- (PMDF V6.4 #31561) id {0KUT00G01CXC5E-at-emroute1.ornl.gov} for
10, 25 -- microscopy-at-microscopy.com; Thu, 17 Dec 2009 15:29:36 -0500 (EST)
10, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60])
10, 25 -- by emroute1.ornl.gov (PMDF V6.4 #31561)
10, 25 -- with ESMTP id {0KUT0027CCXCQA-at-emroute1.ornl.gov} for
10, 25 -- microscopy-at-microscopy.com; Thu, 17 Dec 2009 15:29:36 -0500 (EST)
10, 25 -- Date: Thu, 17 Dec 2009 15:29:36 -0500
10, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
10, 25 -- Subject: SPM/STEM Postdoctoral position open - Local Electrochemical Processes
10, 25 -- in Energy Storage Materials
10, 25 -- To: microscopy-at-microscopy.com
10, 25 -- Message-id: {4B2A94B0.8030404-at-ornl.gov}
10, 25 -- MIME-version: 1.0
10, 25 -- Content-type: text/plain; charset=windows-1252; format=flowed
10, 25 -- Content-transfer-encoding: 8BIT
10, 25 -- User-Agent: Thunderbird 2.0.0.23 (Windows/20090812)
==============================End of - Headers==============================




From: AJBowling-at-dow.com
Date: Fri, 18 Dec 2009 08:53:23 -0600
Subject: [Microscopy] Butyl/Methyl Methacrylate - bubbles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gary.nichols-at-pfizer.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gary.nichols-at-pfizer.com
Name: Gary Nichols

Organization: Pfizer Global R&D (UK)

Title-Subject: [Filtered] Calculation of average atomic number (feedback)

Question: Dear Listers,

Last week I posed the following question and I would now like to
provide feedback from the many responses I got.

Question: When observing backscattered electron images, the relative
brightness of objects, such as particles, is (we are lead to believe)
a function of the average atomic number (av.Z), so that bright
objects have a high av.Z relative to darker ones having a lower av.Z.
This is taken as read by users of BS detectors and is a well known
phenomenon that is described in books and papers. What I would like
to know is how to calculate the av.Z for a material (e.g., a mineral,
an alloy, or an organic compound). I don't like to assume that
specimens that I am viewing have a high or low av.Z just by using the
relative brightness between objects.

Feedback: I want to thank the Listers who took the time and effort
to respond to my question with useful guidance and some very
comprehensive answers. I wasn't trying to attempt to derive a BS
coefficient value from an image, but was trying to calculate a value
from the compound formula that could be used to assess images to
confirm if the relative brightnesses observed made sense.

It became obvious from the responses I received that there are
several ways to calculate the av.Z for a given compound. The
simplest is to sum all the Z numbers and divide the total by the
number of atoms in the formula (e.g., SiO2 = 14+8+8 = 30/3 = 10) to
highly complex Monte-Carlo simulations to calculate values for
accurate quantitative analyses.

For use with electron beam analysis, the method suggested most
frequently by experienced Listers was to use the calculation
described by Goldstein et al (in the book "SEM and X-ray
Microanalysis) and that is to calculate the backscatter coefficient
of a homogeneous compound from the sum of the elemental coefficients
weighted by the weight (mass) concentrations:

av.Z = (w(atom 1)xZ(atom 1))+(w(atom 2)xZ(atom 2))....

This is the calculation I am now using because it is appropriate for
interpreting contrast in BS images.

It also became obvious from some responses that the brightness of
objects viewed using BS imaging (such as grains in a specimen) can
vary due to electron channelling contrast caused by adjacent grains
in different orientations.

Regards
Gary

Login Host: 86.133.82.220
---------------------------------------------------------------------------

==============================Original Headers==============================
15, 11 -- From zaluzec-at-microscopy.com Thu Dec 17 20:10:08 2009
15, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBI2A65G022102
15, 11 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2009 20:10:07 -0600
15, 11 -- Mime-Version: 1.0
15, 11 -- Message-Id: {p06240800c75094e53654-at-[206.69.208.22]}
15, 11 -- Date: Thu, 17 Dec 2009 20:10:05 -0600
15, 11 -- To: microscopy-at-microscopy.com
15, 11 -- From: gary.nichols-at-pfizer.com (by way of MicroscopyListserver)
15, 11 -- Subject: viaWWW: Calculation of average atomic number (feedback)
15, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 3drgenn-at-amanresorts.com Fri Dec 18 03:08:48 2009
Return-Path: {3drgenn-at-amanresorts.com}
Received: from device.lan ([78.93.89.116])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBI98cZ0016123;
Fri, 18 Dec 2009 03:08:41 -0600
Received: from [209.159.24.172] (account r0810-at-aist.go.jp HELO jgyaegtqugbvcb.wcypqlextlmcdof.info)
by device.lan (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 306665708 for microscopy-at-microscopy.com; Fri, 18 Dec 2009 01:08:39 -0800
Message-ID: {0775847948.41HM2TP3321797-at-omazrlwdwocnu.cwrncczlu.net}

Hello everyone,

I am trying to embed some small root tips in butyl/methyl methacrylate.
I am using a ratio of 4:1, and benzoin methyl ether (BME) as the UV
catalyst. I polymerize at room temperature overnight in flat-bottomed
polyethylene and/or polypropylene capsules with indirect illumination
from a UVGL-25 hand lamp at 366nm. I keep getting air bubbles around the
specimen in the finished block. I always vacuum fix these guys until
there is no air was left in them (they look beautiful in LR white). I
have reduced the amount of BME in the final resin from 0.5% to 0.1% to
try to slow down the polymerization. I have also tried up to 6 sheets of
Parafilm, but I still get bubbles. If any of you have suggestions, I
would love to hear them!

Thanks,

Andy Bowling
Imaging Scientist
Dow AgroSciences


==============================Original Headers==============================
5, 31 -- From AJBowling-at-dow.com Fri Dec 18 08:53:23 2009
5, 31 -- Received: from mail171.messagelabs.com (mail171.messagelabs.com [216.82.253.243])
5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBIErMHd028354
5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 18 Dec 2009 08:53:23 -0600
5, 31 -- X-VirusChecked: Checked
5, 31 -- X-Env-Sender: AJBowling-at-dow.com
5, 31 -- X-Msg-Ref: server-11.tower-171.messagelabs.com!1261147982!23548187!16
5, 31 -- X-StarScan-Version: 6.2.4; banners=-,-,-
5, 31 -- X-Originating-IP: [204.136.184.20]
5, 31 -- Received: (qmail 2148 invoked from network); 18 Dec 2009 14:53:19 -0000
5, 31 -- Received: from maila.dow.com (HELO USFRPMDOWS002.dow.com) (204.136.184.20)
5, 31 -- by server-11.tower-171.messagelabs.com with RC4-SHA encrypted SMTP; 18 Dec 2009 14:53:19 -0000
5, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USFRPMDOWS002.dow.com with Microsoft SMTPSVC(6.0.3790.3959);
5, 31 -- Fri, 18 Dec 2009 08:53:13 -0600
5, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 31 -- Content-class: urn:content-classes:message
5, 31 -- MIME-Version: 1.0
5, 31 -- Content-Type: text/plain;
5, 31 -- charset="us-ascii"
5, 31 -- Subject: Butyl/Methyl Methacrylate - bubbles
5, 31 -- Date: Fri, 18 Dec 2009 09:53:08 -0500
5, 31 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1014D638F-at-USMDLMDOWX032.dow.com}
5, 31 -- X-MS-Has-Attach:
5, 31 -- X-MS-TNEF-Correlator:
5, 31 -- Thread-Topic: Butyl/Methyl Methacrylate - bubbles
5, 31 -- Thread-Index: Acp/8c/N0SwvKjXIR9yXVkVwfyjxIw==
5, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com}
5, 31 -- To: {microscopy-at-microscopy.com}
5, 31 -- X-OriginalArrivalTime: 18 Dec 2009 14:53:13.0625 (UTC) FILETIME=[D2EFB090:01CA7FF1]
5, 31 -- Content-Transfer-Encoding: 8bit
5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBIErMHd028354
==============================End of - Headers==============================




From: lkerr-at-mbl.edu
Date: Tue, 22 Dec 2009 08:46:31 -0600
Subject: [Microscopy] viaWWW: Need Philips 300 Service Northeast US

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Andy,
Yes, bubbles can be infuriating with this resin mixure. My
understanding is that the bubbles are formed during polymerization
from the resin, not the sample. The good news about that is it makes
it possible to troubleshoot without samples. I have solved this
problem for myself and for some others by playing around with the
relation between the lamp and the samples. You didn't describe the
relation between the lamp and samples, or what the role of the
parafilm is. I use al foil to block any rays from going direct
between lamp and sample. I have the bulb in the bottom of the box
underneath a piece of plexi. I have a strip of foil running down the
plexi on which I stand the sample capsules. I put a tent of foil over
the row of samples. This doesn't eliminate bubbles but it forces them
to the center and top of the capsule, well away from the sample.
Also, I put the whole operation at 4C. This is beneficial because
polymerization is quite exothermic and the heat load is much less at
4C than at room temp.

Unfortunately, I don't know the physics behind these bubbles
so I cannot suggest anything rational. Just to try different light -
sample arrangments. It does not require much uv light.

Hope some of this helps. Feel free to reply with further questions.

Tobias


} Hello everyone,
}
} I am trying to embed some small root tips in butyl/methyl methacrylate.
} I am using a ratio of 4:1, and benzoin methyl ether (BME) as the UV
} catalyst. I polymerize at room temperature overnight in flat-bottomed
} polyethylene and/or polypropylene capsules with indirect illumination
} from a UVGL-25 hand lamp at 366nm. I keep getting air bubbles around the
} specimen in the finished block. I always vacuum fix these guys until
} there is no air was left in them (they look beautiful in LR white). I
} have reduced the amount of BME in the final resin from 0.5% to 0.1% to
} try to slow down the polymerization. I have also tried up to 6 sheets of
} Parafilm, but I still get bubbles. If any of you have suggestions, I
} would love to hear them!
}
} Thanks,
}
} Andy Bowling
} Imaging Scientist
} Dow AgroSciences
}
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
7, 21 -- From baskin-at-bio.umass.edu Fri Dec 18 09:19:17 2009
7, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBIFJH8T011388
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 18 Dec 2009 09:19:17 -0600
7, 21 -- Received: from [172.30.52.170] (eutopia.bio.umass.edu [128.119.55.30])
7, 21 -- (authenticated bits=0)
7, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id nBIFJCBR001630
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 21 -- Fri, 18 Dec 2009 10:19:13 -0500 (EST)
7, 21 -- Mime-Version: 1.0
7, 21 -- Message-Id: {p06240504c7514b88a5d0-at-[172.30.52.170]}
7, 21 -- In-Reply-To: {200912181454.nBIEs4cg029099-at-ns.microscopy.com}
7, 21 -- References: {200912181454.nBIEs4cg029099-at-ns.microscopy.com}
7, 21 -- Date: Fri, 18 Dec 2009 10:19:11 -0500
7, 21 -- To: AJBowling-at-dow.com
7, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
7, 21 -- Subject: Re: [Microscopy] Butyl/Methyl Methacrylate - bubbles
7, 21 -- Cc: microscopy-at-microscopy.com
7, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
7, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Fri, 18 Dec 2009 10:19:14 -0500 (EST)
7, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19
==============================End of - Headers==============================

From 12thforeportseed-at-foreport.com.tw Fri Dec 18 10:27:38 2009
Return-Path: {12thforeportseed-at-foreport.com.tw}
Received: from [189.32.196.75] (bd20c44b.virtua.com.br [189.32.196.75] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBIGRaDE028226;
Fri, 18 Dec 2009 10:27:37 -0600
Received: from [96.165.111.73] (helo=ofmwfvtqcddg.ugaocywneq.net)
by with esmtpa (Exim 4.69)
(envelope-from )
id 1MML7X-7137bw-LL
for microscopy-at-microscopy.com; Fri, 18 Dec 2009 13:27:35 -0300

Dear Randy

When working with images in DigitalMicrograph it is important to make a
distinction between the raw data (the greyscale values of each pixel), and
what you see displayed on the screen - the image display. The two are not
generally the same. If you right click on an image in DigitalMicrograph and
select Image Display, you can set how the image is displayed via the Survey
method. You can also do it manually, via the histogram. DigitalMicrograph,
generally does a good job of displaying an image, even where the greyscales
in the histogram are bunched up at one end, since it will automatically
stretch the histogram in the display (depending on which survey method is
selected).

When you export this image to another software package, it will most likely
display the image so that the full range of allowable greyscales (which
depends on the bit depth the image was saved), is displayed. This may
generate the washed out images you reported. If your images have been
exported in 16 bit depth, then simply restretching the histogram should
produce a high contrast image. If you exported in 8 bit depth then
stretching the histogram may give a poor result, as there not enough
greyscales in the final result to reproduce tonal variations.

There are a number of options:

Use Gatan's Batch Convert - this will save the image display (ie how it is
set up to appear in DigitalMicrograph) to an 8 bit indexed colour image.

Use my Multliple Saves as Hi-Res TIFF script. This seems to do pretty much
the same as the above, though I haven't compared the two methods. Get it
from: http://www.dmscripting.com/multiple_saves_as_hi-res_tiff.html. I use
this for all my conversions, and haven't had any problems. If you manually
adjust images immediately after acquisition, make sure you save these
changes before attempting either of the above conversions.

(Note the option File/Save Display As - will do the same as the above - save
the front-most image as it is currently displayed, in an 8 bit format.)

Choose File/Save As. If you select the TIFF option you can save your image
in 16 bit format. This preserves greyscale information well, but you will be
limited in the number of applications which will read the file, and you will
have to play around with the levels to duplicate what was shown in the image
display.

Use a plugin for ImageJ and read your DM files directly - I haven't really
played with this as I do everything in DigitalMicrograph.

Hope this helps.

Happy holidays.

Regards

Dave Mitchell


Dr David Mitchell
Senior Microscopist, Transmission Electron Microscopy

Contact:
PH +61 2 9036 7633
FAX +61 2 9351 7682
David.mitchell-at-emu.usyd.edu.au

Address:
Electron Microscope Unit
Australian Key Centre for Microscopy and Microanalysis
Australian Microscopy & Microanalysis Research Facility (AMMRF)
Madsen Building F09, Room 111A
The University of Sydney
NSW 2006, Australia
www.emu.usyd.edu.au
www.ammrf.org.au

www.dmscripting.com



==============================Original Headers==============================
20, 27 -- From david.mitchell-at-usyd.edu.au Sun Dec 20 20:07:14 2009
20, 27 -- Received: from london.ucc.usyd.edu.au (london.ucc.usyd.edu.au [129.78.220.2])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBL27DeV009673
20, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 20 Dec 2009 20:07:14 -0600
20, 27 -- Received: from london.ucc.usyd.edu.au (localhost [127.0.0.1])
20, 27 -- by localhost (Postfix) with SMTP id F045A2CAC09;
20, 27 -- Mon, 21 Dec 2009 13:07:11 +1100 (EST)
20, 27 -- Received: from ex-bri-pro-02.mcs.usyd.edu.au (ex-bri-pro-02.mcs.usyd.edu.au [172.17.63.242])
20, 27 -- by london.ucc.usyd.edu.au (Postfix) with ESMTP id E2D442CABF2;
20, 27 -- Mon, 21 Dec 2009 13:07:11 +1100 (EST)
20, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.2]) by ex-bri-pro-02.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.1830);
20, 27 -- Mon, 21 Dec 2009 13:07:12 +1100
20, 27 -- Received: from 172.17.185.135 ([172.17.185.135]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.6]) with Microsoft Exchange Server HTTP-DAV ;
20, 27 -- Mon, 21 Dec 2009 02:07:11 +0000
20, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122
20, 27 -- Date: Mon, 21 Dec 2009 13:07:10 +1100
20, 27 -- Subject: [Microscopy] Digital Micrograph image conversion
20, 27 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au}
20, 27 -- To: {Microscopy-at-microscopy.com} , {TindallR-at-missouri.edu}
20, 27 -- Message-ID: {C755237E.2285%david.mitchell-at-emu.usyd.edu.au}
20, 27 -- Thread-Topic: [Microscopy] Digital Micrograph image conversion
20, 27 -- Thread-Index: AcqB4k24jBlTru3VEd6ESwAjMrpsqg==
20, 27 -- Mime-version: 1.0
20, 27 -- Content-type: text/plain;
20, 27 -- charset="US-ASCII"
20, 27 -- Content-transfer-encoding: 7bit
20, 27 -- X-OriginalArrivalTime: 21 Dec 2009 02:07:12.0248 (UTC) FILETIME=[4F0F5F80:01CA81E2]
==============================End of - Headers==============================

From h1089dd-at-ms21.hinet.net Sun Dec 20 23:11:00 2009
Return-Path: {h1089dd-at-ms21.hinet.net}
Received: from [89.179.216.135] ([89.179.216.135])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBL5Ax13028426;
Sun, 20 Dec 2009 23:10:59 -0600
Received: from [27.177.119.4] (account h1089dd-at-ms21.hinet.net HELO lzjgnmfaosol.surrna.tv)
by (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 408650034 for microscopy-at-microscopy.com; Mon, 21 Dec 2009 08:10:59 +0300

The University of Missouri is looking for an ASSOCIATE DIRECTOR for a campus-wide light microscopy imaging facility. Applicants should have experience in some or all of the following areas:
* confocal laser scanning microscopy
* laser capture microdissection
* bright field microscopy
* DIC/Nomarski and phase contrast microscopy
* wide field fluorescence microscopy
* Two-photon microscopy
* deconvolution
* FRET
* Spectral imaging/unmixing
* image processing/analysis
* all types of microtomy
* immunocytochemistry
* in situ hybridization

The Associate Director will be responsible for training users, maintaining instruments and developing protocols for a campus-wide multi-user facility. PhD desirable but not required for individuals with extensive experience. Although an ideal candidate would have experience in all of the areas listed above, candidates with extensive experience in selected areas and the desire and capacity to learn the additional areas will be considered. Excellent oral and written communication skills are essential. Experience in a multi-user core facility would be viewed positively. Electron microscopy is not a component of this core facility. Women and minority candidates are especially encouraged to apply. Review of applications will begin immediately and continue until an appropriate candidate is hired. This is a full-time, benefit eligible position. Opportunities for professional development will be encouraged.

The Core's web site can be viewed at http://www.biotech.missouri.edu/mcc/

Available instrumentation includes:
* Zeiss LSM 510 META NLO two-photon confocal system
* Zeiss LSM 5 LIVE confocal system
* BioRad Radiance 2000 confocal system
* Molecular Devices ArcturusXT laser capture microdissection system
* Leica DMI4000B with Eppendorf microjection system
* Olympus IX70 and Nikon Optiphot 2 widefield systems
* Olympus Vanox AHBT3 upright microscope and color digital camera
* Leica stereo microscope equipped with epi-fluorescence excitation and color camera
* Olympus SZX12 stereo microscope with color digital camera
* Intavis InsituPro VS in situ hybridization robotic system
* Autodeblur deconvolution software (Autoquant Inc.).
* Molecular Devices Metamorph, Bitplane Imaris, and ImagePro Plus image acquisition, analysis, and processing software.
* Leica ultramicrotome, cryostat, paraffin microtomes, Vibratome 3000

The University of Missouri has over 30,000 students and is located in Columbia, Missouri. Columbia is a classic college town with a 100,000+ population and has a lower cost of living than most places in America. Columbia has been selected as one of America's best places to live by Money magazine, Men's Journal and MSN.com. Further information about Columbia can be found at http://gradschool.missouri.edu/admission/relocating.php.

Address applications (CV and 3 letters of reference) or inquires to:

Thomas E. Phillips, Ph.D.
Division of Biological Sciences
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712
phillipst-at-missouri.edu



==============================Original Headers==============================
9, 36 -- From PhillipsT-at-missouri.edu Mon Dec 21 10:39:19 2009
9, 36 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49])
9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBLGdJSR017217
9, 36 -- for {microscopy-at-microscopy.com} ; Mon, 21 Dec 2009 10:39:19 -0600
9, 36 -- X-IronPort-Anti-Spam-Filtered: true
9, 36 -- X-IronPort-Anti-Spam-Result: AqwFAK4zL0vRauUp/2dsb2JhbACcOIgNpWgBCYQTiA6CSoFkBIFl
9, 36 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41])
9, 36 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 21 Dec 2009 10:39:19 -0600
9, 36 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959);
9, 36 -- Mon, 21 Dec 2009 10:39:19 -0600
9, 36 -- Received: from um-email05.um.umsystem.edu ([169.254.1.61]) by
9, 36 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Mon, 21 Dec 2009
9, 36 -- 10:39:18 -0600
9, 36 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu}
9, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
9, 36 -- Date: Mon, 21 Dec 2009 10:39:16 -0600
9, 36 -- Subject: Associate Director position
9, 36 -- Thread-Topic: Associate Director position
9, 36 -- Thread-Index: AcqCXCKJS91aHj4XR8+gC+J4oE2fPg==
9, 36 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF07E45A6AA2-at-UM-EMAIL05.um.umsystem.edu}
9, 36 -- Accept-Language: en-US
9, 36 -- Content-Language: en-US
9, 36 -- X-MS-Has-Attach:
9, 36 -- X-MS-TNEF-Correlator:
9, 36 -- x-cr-hashedpuzzle: Acug Cu9+ CyT5 CzoB C5H3 DHPj D6q5 FqFh GJ3r GWbh G2ha
9, 36 -- HBYT HYsa Ie7C Jj6z
9, 36 -- KPsN;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{74111841-683D-4C4E-AE94-9BDD02279E41};cABoAGkAbABsAGkAcABzAHQAQABtAGkAcwBzAG8AdQByAGkALgBlAGQAdQA=;Mon,
9, 36 -- 21 Dec 2009 16:39:16
9, 36 -- GMT;QQBzAHMAbwBjAGkAYQB0AGUAIABEAGkAcgBlAGMAdABvAHIAIABwAG8AcwBpAHQAaQBvAG4A
9, 36 -- x-cr-puzzleid: {74111841-683D-4C4E-AE94-9BDD02279E41}
9, 36 -- acceptlanguage: en-US
9, 36 -- Content-Type: text/plain; charset="us-ascii"
9, 36 -- MIME-Version: 1.0
9, 36 -- X-OriginalArrivalTime: 21 Dec 2009 16:39:19.0055 (UTC) FILETIME=[244479F0:01CA825C]
9, 36 -- Content-Transfer-Encoding: 8bit
9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBLGdJSR017217
==============================End of - Headers==============================

From a.burkes-at-adamjeeinsurance.com Mon Dec 21 12:37:36 2009
Return-Path: {a.burkes-at-adamjeeinsurance.com}
Received: from nat233-out.convex.ru (nat233-out.convex.ru [82.193.139.233] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBLIbY67003156;
Mon, 21 Dec 2009 12:37:35 -0600
Received: from [152.60.14.199] (helo=isgnzszrwn.fsqfuzdgkm.com)
by nat233-out.convex.ru with esmtpa (Exim 4.69)
(envelope-from )
id 1MMZSZ-8513ri-GH
for microscopy-at-microscopy.com; Mon, 21 Dec 2009 23:37:34 +0500
Message-ID: {3749477356.FS5NTZTZ480994-at-mvfmvfgzxpffyxh.yorcmjrfkvlfr.tv}

We are offering our Kevex Delta Class EDS Analyzer for sale, maybe donation, before committing the unit to surplus. Some more information on the unit is available here: http://ahrenkiel.sdsmt.edu/H7000_TEM/surplus/

The price is negotiable, but the recipient must pay shipping. The detector is not part of the offer. (We are still using it.)

If interested, please contact me directly.
------------------------------------------
Phil Ahrenkiel, Assistant Professor
Nanoscience and Nanoengineering Ph.D. Program
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701
Office: EP 221
Phone: 605-394-5238, Fax: 605-394-2365
Email: Phil.Ahrenkiel-at-sdsmt.edu



==============================Original Headers==============================
5, 41 -- From Phil.Ahrenkiel-at-sdsmt.edu Mon Dec 21 14:23:11 2009
5, 41 -- Received: from silver.sdsmt.edu (silver.sdsmt.edu [151.159.1.1])
5, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBLKNBTK007891
5, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Dec 2009 14:23:11 -0600
5, 41 -- Received: from sdsmt-ex07.sdsmt.edu (sdsmt-ex07.sdsmt.edu [151.159.3.6])
5, 41 -- by silver.sdsmt.edu (8.14.3/8.14.3) with ESMTP id nBLKNBRL006385
5, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Dec 2009 13:23:11 -0700
5, 41 -- DomainKey-Signature: a=rsa-sha1; s=sdsmtdk; d=sdsmt.edu; c=nofws; q=dns;
5, 41 -- h=dkim-signature:received:from:to:date:subject:thread-topic:
5, 41 -- thread-index:message-id:accept-language:content-language:
5, 41 -- x-ms-has-attach:x-ms-tnef-correlator:acceptlanguage:content-type:
5, 41 -- content-transfer-encoding:mime-version;
5, 41 -- b=W1kMyVq2TqxR161TUOgeGEfiaJumhnLbIMbg5dz5XyCVcij9ZwyAW+1/KsYq3bvO/
5, 41 -- XsfWZfI/7Isql0BLt490rVaDmA9DGuAT0hFW+uL5V0B5WOZqSYoTz5WoNDzqvaRYp1j
5, 41 -- mMpp15seS/SqT3Zvnsqv3Yr+teS1/VQSq+dDHXg=
5, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu;
5, 41 -- s=sdsmtdkim; t=1261426991;
5, 41 -- bh=n2rKrME6UOV3FVAUqbeVMobLN8EtB9qKixHsRL0D3YU=;
5, 41 -- h=From:To:Date:Subject:Message-ID:Content-Type:
5, 41 -- Content-Transfer-Encoding:MIME-Version;
5, 41 -- b=m+OjnsouV9/XjCNV61NES4++OqHYst4V/FgWUYI3RtJXkl3NPJA6sBiJnRJMpauSo
5, 41 -- L08L1DQCytKvJ+WvRwHc+h1ZTdBzHL0hSSJA7QE8awvZStfHiQrNG/VqFeiVqGoG/K
5, 41 -- Pd/0BPq67rB/862O5CU+uAFp8KWs1+hy9yGAtmuY=
5, 41 -- Received: from sdsmt-ex07.SDSMT.local ([151.159.3.6]) by sdsmt-ex07
5, 41 -- ([151.159.3.6]) with mapi; Mon, 21 Dec 2009 13:23:11 -0700
5, 41 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu}
5, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com}
5, 41 -- Date: Mon, 21 Dec 2009 13:23:11 -0700
5, 41 -- Subject: Kevex EDS system
5, 41 -- Thread-Topic: Kevex EDS system
5, 41 -- Thread-Index: AcqCe2pbPfiBQuzdTD26s1x0QtQa2Q==
5, 41 -- Message-ID: {0E8C875976EF6C4FAD2BE0CEE93BD2D608C3942360-at-sdsmt-ex07}
5, 41 -- Accept-Language: en-US
5, 41 -- Content-Language: en-US
5, 41 -- X-MS-Has-Attach:
5, 41 -- X-MS-TNEF-Correlator:
5, 41 -- acceptlanguage: en-US
5, 41 -- Content-Type: text/plain; charset="us-ascii"
5, 41 -- MIME-Version: 1.0
5, 41 -- Content-Transfer-Encoding: 8bit
5, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id nBLKNBTK007891
==============================End of - Headers==============================

From 3005897u4414f95a-at-bridgestone.co.jp Mon Dec 21 15:13:20 2009
Return-Path: {3005897u4414f95a-at-bridgestone.co.jp}
Received: from [213.176.231.33] ([213.176.231.33])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBLLDIRF024257;
Mon, 21 Dec 2009 15:13:19 -0600
Received: from [37.178.195.191] (helo=moxmuxtofiin.pddpvnphioi.com)
by with esmtpa (Exim 4.69)
(envelope-from )
id 1MM7B9-5836op-HM
for microscopy-at-microscopy.com; Tue, 22 Dec 2009 00:13:19 +0300

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wei.kong-at-oregonstate.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: wei.kong-at-oregonstate.edu
Name: Wei Kong

Organization: Oregon State University

Title-Subject: [Filtered] Postdoctoral position in instrumental
development for coherent electron diffraction

Question: An immediate opening for a postdoctoral position is
available at Oregon State University. The project involves electron
diffraction of laser oriented molecules embedded in superfluid helium
droplets. Experience in coherent x-ray or electron diffraction is
preferred but not mandated. The individual needs to have a solid
background in physics, optics, and electronics, and extensive hands
on experience in one of the fields. Intellectual curiosity and
willingness to step into unfamiliar disciplines are essential
qualities. Candidates should send their curriculum vitae with 3
reference letters to Wei Kong, Department of Chemistry, Oregon State
University, Corvallis, OR 97331. The position is open until filled.
Oregon State University is an AA/EOE.

Login Host: 128.193.93.204
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Mon Dec 21 20:02:12 2009
6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBM22BCu012054
6, 12 -- for {microscopy-at-microscopy.com} ; Mon, 21 Dec 2009 20:02:12 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- Message-Id: {p06240802c755d91036a0-at-[206.69.208.22]}
6, 12 -- Date: Mon, 21 Dec 2009 20:02:10 -0600
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: wei.kong-at-oregonstate.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Postdoctoral position in instrumental development for
6, 12 -- coherent electron diffraction
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 1sdd-at-ms2.hinet.net Mon Dec 21 20:42:16 2009
Return-Path: {1sdd-at-ms2.hinet.net}
Received: from host-70-45-114-186.onelinkpr.net (host-70-45-114-186.onelinkpr.net [70.45.114.186])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBM2gFrf027481;
Mon, 21 Dec 2009 20:42:16 -0600
Received: from [4.175.161.153] (account 1sdd-at-ms2.hinet.net HELO wlobatdro.qbtvxdwc.info)
by host-70-45-114-186.onelinkpr.net (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 326016708 for microscopy-at-microscopy.com; Mon, 21 Dec 2009 22:42:15 -0400

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lkerr-at-mbl.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: lkerr-at-mbl.edu
Name: Louis Kerr

Organization: Marine Biological Laboratory

Title-Subject: [Filtered] Philips 300 Service Northeast US

Question: I am posting this for a colleague.

Can anyone recommend a service engineer for a Philips 300 TEM located
in Providence, Rhode Island? If so please provide the contact
information to:
Dr. Joseph A. DeGiorgis
Providence College
Providence RI,
jdegiorg-at-providence.edu
508-292-4605

Happy holidays,
Louie

Login Host: 76.119.200.182
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Dec 22 08:46:30 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBMEkTow027797
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 22 Dec 2009 08:46:30 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240800c7568c2442c8-at-[206.69.208.22]}
8, 11 -- Date: Tue, 22 Dec 2009 08:46:29 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: lkerr-at-mbl.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Need Philips 300 Service Northeast US
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: Ulysses Brunson :      3dagriman10-at-doae.go.th
Date: Fri, 1 Jan 2010 16:42:50 +0800
Subject: ??????? ????? ????????. ???????? ??????

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Gregory.Hendricks-at-umassmed.edu as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Gregory.Hendricks-at-umassmed.edu
Name: Gregory Hendricks

Organization: University of Massachusetts Medical School

Title-Subject: [Filtered] Philips CM 12

Question: We are offering our Philips CM 12 for sale, maybe donation,
before committing the unit to surplus. Some more information on the
unit is available here:
http://www.umassmed.edu/cemf/equipment.aspx?linkidentifier=id&itemid=2998The
price is negotiable, but the recipient must pay shipping. The Gatan
digital camera system is not part of the offer. (We are still using
it.)

If interested, please contact me directly.
------------------------------------------
Greg Hendricks, Assistant Professor
Core Electron Microscopy, Cell Biology
University of Massachusetts Medical School
Worcester, MA 01655
Office: SA-114
Phone: 508-856-2602, Fax: 508-856-5612
Email: Gregory.Hendricks-at-umassmed.edu


Login Host: 146.189.246.45
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 11 -- From zaluzec-at-microscopy.com Tue Dec 22 08:47:22 2009
8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBMElHsn028562
8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 22 Dec 2009 08:47:21 -0600
8, 11 -- Mime-Version: 1.0
8, 11 -- Message-Id: {p06240801c7568c5d505c-at-[206.69.208.22]}
8, 11 -- Date: Tue, 22 Dec 2009 08:47:16 -0600
8, 11 -- To: microscopy-at-microscopy.com
8, 11 -- From: Gregory.Hendricks-at-umassmed.edu (by way of MicroscopyListserver)
8, 11 -- Subject: viaWWW: Philips CM 12 Available
8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 1umin08f-at-ms2.hinet.net Tue Dec 22 09:06:55 2009
Return-Path: {1umin08f-at-ms2.hinet.net}
Received: from 93-47-27-82.ip110.fastwebnet.it (93-47-27-82.ip110.fastwebnet.it [93.47.27.82])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBMF6qvo025045;
Tue, 22 Dec 2009 09:06:53 -0600
Received: from [155.41.63.16] (account 1umin08f-at-ms2.hinet.net HELO dlodpwjmdwcxub.lffkgojiqbvvqy.ua)
by 93-47-27-82.ip110.fastwebnet.it (CommuniGate Pro SMTP 5.2.3)
with ESMTPA id 031853330 for microscopylistserverarchive-at-microscopy.com; Tue, 22 Dec 2009 16:06:53 +0100
Message-ID: {1071247835.QRKIQWDK187844-at-oqlbamwm.tsjdozblsjflhl.ua}

Reply directly to Dr. Dong. {dongh-at-muohio.edu}


------- Forwarded message follows -------

X-from: "Dong, Hailiang Dr." {dongh-at-muohio.edu}

Geology: Postdoctoral Fellow to perform mineralogical
characterizations using X-ray diffraction, scanning and transmission
electron microscopy (SEM and TEM), and mineral-microbe interactions;
work in a team in the field including site characterization and core
collection as well as numerical modeling. Require: Ph.D in
mineralogy or materials science; extensive experience in TEM,
electron energy loss spectroscopy (EELS), mineral-microbe
interactions (microbial reduction and oxidation of metals), and wet
chemistry; good oral and written communication skills. Desire:
Experience in synchrotron-based techniques, Mossbauer spectroscopy,
molecular microbiology, microbial ecology, and/or metagenomics and
microarrays. This is a one-year appointment with re-appointment
subject to continued funding and satisfactory performance. Send
letter of application, curriculum vitae, and names and contact
information for three references to Dr. Hailiang Dong, Department of
Geology, Miami University, Oxford, OH 45056. Contact phone number is
513-529-2517; email is dongh-at-muohio.edu. Review of applications will
begin as soon as possible and will continue until the position is
filled. Miami University is an affirmative action/equal opportunity
employer with smoke-free campuses. For information regarding campus
crime and safety, visit www.muohio.edu/righttoknow.

Hailiang Dong - Professor
Department of Geology
Miami University
Oxford, OH 45056
Tel: 513-529-2517 (office)
Tel: 513-529-6025 (lab)
Fax: 513-529-1542
http://www.users.muohio.edu/dongh/


------- End of forwarded message -------

==============================Original Headers==============================
8, 22 -- From edelmare-at-muohio.edu Tue Dec 22 09:27:03 2009
8, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.71])
8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBMFR38w026199
8, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Dec 2009 09:27:03 -0600
8, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11])
8, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id nBMFR6Et005718
8, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Dec 2009 10:27:06 -0500
8, 22 -- Received: from [10.34.160.234] ([10.34.160.234])
8, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id nBMFR3TN026343
8, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Dec 2009 10:27:03 -0500
8, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu}
8, 22 -- To: microscopy-at-Microscopy.com
8, 22 -- Date: Tue, 22 Dec 2009 10:27:03 -0500
8, 22 -- MIME-Version: 1.0
8, 22 -- Subject: Post-doc Position Available
8, 22 -- Message-ID: {4B309EF7.3076.548660C-at-edelmare.muohio.edu}
8, 22 -- Priority: normal
8, 22 -- X-mailer: Pegasus Mail for Windows (4.41)
8, 22 -- Content-type: text/plain; charset=US-ASCII
8, 22 -- Content-transfer-encoding: 7BIT
8, 22 -- Content-description: Mail message body
8, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.71
==============================End of - Headers==============================

From an-at-matsunaga.com Tue Dec 22 10:17:50 2009
Return-Path: {an-at-matsunaga.com}
Received: from 87-121-149-164.telecablenet.com (87-121-149-164.telecablenet.com [87.121.149.164])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBMGHlOa010306;
Tue, 22 Dec 2009 10:17:48 -0600
Received: from [192.102.74.66] (helo=tlltnjvc.ihlrlwv.ua)
by 87-121-149-164.telecablenet.com with esmtpa (Exim 4.69)
(envelope-from )
id 1MMT49-0752yq-93
for microscopylistserverarchive-at-microscopy.com; Tue, 22 Dec 2009 18:17:47 +0200

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both panxijiang-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: panxijiang-at-gmail.com
Name: Xijiang Pan

Organization: Tsinghua University

Title-Subject: [Filtered] how to transform the ccd image acquired by
FEI eagle ccd (*.emi) format to mrc format

Question: we are now using a FEI Eagle CCD to acquire the EM images,
and this format of the ccd image (*.emi) is not supported by the
single particle analysis software like SPIDER and EMAN, and we
currently do not know the details of this format. Does anyone have
any experiences on how to transform this format to MRC format, or the
detailed informations about the emi format information.

thanks.

happy holiday!

Login Host: 166.111.30.39
---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Wed Dec 23 16:18:13 2009
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBNMICQM021233
8, 12 -- for {microscopy-at-microscopy.com} ; Wed, 23 Dec 2009 16:18:13 -0600
8, 12 -- Mime-Version: 1.0
8, 12 -- Message-Id: {p06240800c758478e31bd-at-[206.69.208.22]}
8, 12 -- Date: Wed, 23 Dec 2009 16:18:10 -0600
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: panxijiang-at-gmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: how to transform the ccd image acquired by FEI eagle ccd
8, 12 -- (*.emi) format to mrc
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From alamzohur-at-youngone.co.kr Wed Dec 23 17:23:45 2009
Return-Path: {alamzohur-at-youngone.co.kr}
Received: from 201-167-9-165-cable.cybercable.net.mx (201-167-9-165-cable.cybercable.net.mx [201.167.9.165])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBNNNiwA005703
for {microscopylistserverarchive-at-microscopy.com} ; Wed, 23 Dec 2009 17:23:44 -0600
Received: from [36.49.136.2] (helo=yfvokrdosnkp.ssmxqulycmpmv.biz)
by 201-167-9-165-cable.cybercable.net.mx with esmtpa (Exim 4.69)
(envelope-from )
id 1MMW1R-5168pj-QK
for microscopylistserverarchive-at-microscopy.com; Wed, 23 Dec 2009 17:23:44 -0600
Message-ID: {6906373707.0OU48OJR029078-at-iqfunpimxw.mhxayq.su}


Dear All,

I would like to find out if there is an easy and effective procedure which
would allow me to prepare samples of cancer cells grown on CNT forests for
observation under SEM. I will be using an CZ EVO 40 (LaB6 filament) and I
have never worked on soft materials.

Happy holidays and new years to you all

Erman Bengu




=================================
Erman Bengu

Assistant Professor of Chemistry
Department of Chemistry
Bilkent University

Mailing Address:
Bilkent University,
Department of Chemistry,
06800, Bilkent, Ankara
Turkey

Office: SB #311
E-mail: bengu_AT_fen.bilkent.edu.tr
Phone (Office): +90 (312) 290-2153
(Lab1): +90 (312) 290-2663
(Lab2): +90 (312) 290-3332
Fax: +90 (312) 266-4068
Web: http://www.fen.bilkent.edu.tr/~bengu
==================================


==============================Original Headers==============================
13, 22 -- From bengu-at-fen.bilkent.edu.tr Thu Dec 24 01:19:02 2009
13, 22 -- Received: from ispinoz.bcc.bilkent.edu.tr (ispinoz.bcc.bilkent.edu.tr [139.179.10.240])
13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBO7J2N1021585
13, 22 -- for {microscopy-at-microscopy.com} ; Thu, 24 Dec 2009 01:19:02 -0600
13, 22 -- Received: from newmail.bilkent.edu.tr (unknown [192.168.10.203])
13, 22 -- by ispinoz.bcc.bilkent.edu.tr (Postfix) with ESMTP id 4A0CE6AE8
13, 22 -- for {microscopy-at-microscopy.com} ; Thu, 24 Dec 2009 09:19:01 +0200 (EET)
13, 22 -- Received: from 139.179.97.107
13, 22 -- (SquirrelMail authenticated user bengu-at-fen.bilkent.edu.tr)
13, 22 -- by newmail.bilkent.edu.tr with HTTP;
13, 22 -- Thu, 24 Dec 2009 09:19:16 +0200 (EET)
13, 22 -- Message-ID: {51571.139.179.97.107.1261639156.squirrel-at-newmail.bilkent.edu.tr}
13, 22 -- Date: Thu, 24 Dec 2009 09:19:16 +0200 (EET)
13, 22 -- Subject: SEM of cancer cells
13, 22 -- From: "Erman Bengu" {bengu-at-fen.bilkent.edu.tr}
13, 22 -- To: microscopy-at-microscopy.com
13, 22 -- User-Agent: SquirrelMail/1.4.13
13, 22 -- MIME-Version: 1.0
13, 22 -- Content-Type: text/plain;charset=iso-8859-9
13, 22 -- Content-Transfer-Encoding: 8bit
13, 22 -- X-Priority: 3 (Normal)
13, 22 -- Importance: Normal
==============================End of - Headers==============================

From e00353-at-mail.trtc.com.tw Thu Dec 24 02:45:16 2009
Return-Path: {e00353-at-mail.trtc.com.tw}
Received: from nat250-out.convex.ru (nat250-out.convex.ru [82.193.139.250] (may be forged))
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBO8jErJ006492;
Thu, 24 Dec 2009 02:45:15 -0600
Received: from [165.108.152.99] (helo=xnmivrdgtyzdlac.hsrnlgnxvireo.ua)
by nat250-out.convex.ru with esmtpa (Exim 4.69)
(envelope-from )
id 1MM2VL-7831de-02
for microscopylistserverarchive-at-microscopy.com; Thu, 24 Dec 2009 13:45:14 +0500

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both watson-at-wi.mit.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: watson-at-wi.mit.edu
Name: Nicki Watson

Organization: Whitehead Institute

Title-Subject: [Filtered] Service Manuals for em410

Question: I have service manuals and electron optics manuals for the
phillips EM 410. I upgraded my scope a few years back and I no longer
need these manuals. If anyone wants them please let me know as soon
as possible. I have to throw away everything I can to make room for
the reorganization of space in my core facility.Please contact Nicki
Watson watson-at-wi.mit.edu

Login Host: 18.4.1.144
---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Tue Dec 29 18:45:19 2009
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBU0jI94021433
6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 29 Dec 2009 18:45:19 -0600
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06240800c760530aec29-at-[206.69.208.22]}
6, 11 -- Date: Tue, 29 Dec 2009 18:45:18 -0600
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: watson-at-wi.mit.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Service Manuals for em410
6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================

From 3dhkawaguchi-at-ltfv.co.jp Wed Dec 30 05:47:57 2009
Return-Path: {3dhkawaguchi-at-ltfv.co.jp}
Received: from sura.ru (host-93-124-91-82.dsl.sura.ru [93.124.91.82])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id nBUBltdD021002;
Wed, 30 Dec 2009 05:47:56 -0600
Received: from [212.52.154.163] (helo=zygwcuxqwithh.qcxsbuciqikss.org)
by sura.ru with esmtpa (Exim 4.69)
(envelope-from )
id 1MMP7A-7779yf-YN
for microscopy-at-microscopy.com; Wed, 30 Dec 2009 14:47:55 +0300




MicroscopyListserver Archive Email Extraction Software Version NJZ07060908

Return to Microscopy Listserver Home Page


Return to MSA HomePage